His pupils were his nephew Bartholomeus Barbiers the younger, and the following students at the academy: Carel Lodewijk Hansen, Jan Hulswit (painter), Johannes Jelgerhuis, Daniël Kerkhoff, Johannes Hendrik Knoop, Thomas van Leent, Jacobus Pelgrom, Petrus Antonius Ravelli, Abraham Johannes Ruytenschildt, Johan Christiaan Willem Safft, Barend Hendrik Thier, Pier Johannes de Visser, and Henricus Franciscus Wiertz.
Az akadémián is számos tanítványa volt, többek között: Carel Lodewijk Hansen, Jan Hulswit, Johannes Jelgerhuis, Daniël Kerkhoff, Johannes Hendrik Knoop, Thomas van Leent, Jacobus Pelgrom, Petrus Antonius Ravelli, Abraham Johannes Ruytenschildt, Johan Christiaan Willem Safft, Barend Hendrik Thier, Pier Johannes de Visser és Henricus Franciscus Wiertz.