Additional considerations: In this experiment, our goal is to establish the steady-state abundance of AMT using a bacterial density similar to the average concentration of microbes on normal skin, approximately 105/cm2. Using the results of Experiment 1, we can estimate the half-life of the bacteria on subject skin surface. It is possible that the AMT cream half-life as determined in Experiment 1 will be short, possibly even as short as a few hours. If the AMT cream does show a rapid loss of transplanted bacteria such that it is impractical to further increase the frequency of application of the AMT (assumed as greater than TID), and the dose of the AMT cannot be practically increased from standard 108 bacteria/gm, applied at 10 gm/m2, the subject will apply the maximal practical dose (109 bacteria/gm at 40gm/m2 ) at the maximal practical frequency (TID), and we will do this for one week to determine how many bacteria are present after this treatment approach. Although this is not likely from the data of Costello et al. in 2009, we want to have this back-up plan in place to allow the study to move forward. Although these results would not represent a steady-state, the data would be sufficient to enable design of a subsequent clinical trial or to conclude that a long term application approach is not worth further analyses.