【Evaluation parameters:】The evaluation parameters include clinical assessment, local blood flow perfusion, bacteriological examination, histopathological evaluation and immunohistochemical analysis from the biopsy specimens. The ulcers are quantitatively assessed using the S(AD)SAD classification for the size, shape and configuration with photo-documentation before and after treatment. The neuropathic sensory change of the foot is examined with pin-prick test. The circulatory status of the affected limb is evaluated with digital palpation of the dorsalis pulsation and ultrasound Doppler perfusion scan. Local blood flow perfusion scan, bacterial culture and sensitivity, and biopsy from the periphary of the ulcer including normal skin is performed before and after treatment, and the specimens are subjected to histomorphological examination and immunohistochemicial stain. The data before and after treatment within the same group and the data between the two groups will be analyzed statistically.