For Universal Donor Stool Preparation we follow the above preparation and then amended thereafter with sterile pharmaceutical grade glycerol (Sigma, St Louis, MO) to a final concentration of 10 % , and stored frozen at − 80 ° C . The stool suspension will be divided into 90ml aliquots. Each 90 ml aliquot will be use separately. Thawing will be done over 2 - 4 h in an ice bath before the FMT procedure. 9 For universal donor screen the same protocol as for non-universal donor screen will be followed, including the aforementioned donor inclusion and exclusion criteria and screening labs.