The vasoconstriction together with the blunt point of the cannula avoids any accidental intravascular injection and facilitates the subsequent lipoaspiration. Three hundred to 500 ml are usually injected in the chosen area for fat harvesting (usually the lower abdomen), making the tissue really "filled" with the injecting solution. The aspiration step (lipoaspirate) is performed by a luer lock syringe connected to a disposable 19-cm blunt cannula (3 mm OD), with 5 oval holes (1 ´ 2 mm). A few strokes using a standard liposuction technique are enough to harvest 6-10 ml of fat tissue. Vacuum while aspirating can be obtained manually or by clamping the syringe plunger with a clamp instrument. Up to 1,000 ml can be harvested in less than 15 min, and 1-2 min are enough. The harvested lipoaspirate can be progressively put into the device using multiple 10-cc syringes.