The grant was renewed in 1996 through August 2001 to conduct a longitudinal study of 726 members of the original cohort. The purpose was to determine the importance of weight gain and weight-related factors, and the possible interaction of other factors, e.g. psychosocial, electrolytes, reactivity, macronutrient intake, to change in blood pressure. Factors related to weight gain were identified. The high prevalence of the electrocardiogram left ventricular hypertrophy (ECG-LVH) was validated against echocardiographic measures (ECHO-LVH). Predictors of change in ECG-LVH, and the correlates of microalbuminuria were identified. In Year 2 (Cohort Year 4) half of the population was restudied with echocardiography, cardiovascular reactivity, and new psychosocial measures. In Year 4 (Cohort Year 6), with the exception of cardiovascular reactivity, the full cohort was re-examined for baseline measures, including multiple blood pressure readings, height, weight, waist, hips, ECG, physical activity, two 24 hour dietary recalls, alcohol intake, menopausal status, psychosocial measures, 24 hour urine for sodium, potassium, creatinine, micro-albuminuria, and fasting serum for lipids, insulin, glucose, and creatinine.