At time of enrollment, 30cc of blood will be obtained: 10cc for plasma banking for neurohormones and cytokine analysis, 10 cc for DNA banking and genotyping, 5 cc for evaluation of gene expression of inflammatory markers from circulating leukocytes and 5 cc for serum banking. Ten ccs of blood for cytokine analysis will also be collected at one month and six months after entry. A blood specimen for repeat analysis of serum mediator (5ccs in a red top tube) will be drawn at six months and sent to the University of Pittsburgh. All blood samples will be kept for 10 years. Access to the banked DNA will be limited to investigations at the University of Pittsburgh and each site of this multi-center trial. These samples will be available without any identifying information (name, social security number and medical record number). In addition, a Certificate of Confidentiality has been obtained from the NIH for further subject protection.