To evaluate the clinical and parasitological outcome on primary blood infection, samples were obtained for days 2. 3, 7, 14, 21 and 28 in a flexible schedule and for recurrent blood infections were continue to sample each month up to 12 months. Blood samples from Day 2 to Day 28 were analyzed by microscopy of the thick smear and by molecular diagnosis. Samples from patient with symptoms were immediately analyzed and if a recurrent infection detected, patients were rescued by the 14-Day treatment. To uncover asymptomatic infections, all other samples within 1-12 months were analyzed by microscopy and tested antibody IgG titers and only those suspected of a recurrent blood infection were assayed by molecular diagnosis.