The study was renewed in 1992 to carry out six aims that 1) provided new information about four polymorphic genes involved in reverse cholesterol transport; 2) completed the investigators' studies to evaluate the utility of genetic variation in 12 candidate genes for prediction of coronary artery disease; 3) identified individuals who carry mutations that have an impact on coronary artery disease; and 4) began molecular studies of these individuals to define the DNA changes that are responsible. New noninvasive measures of coronary artery disease in asymptomatic individuals using ultrafast cardiac computed tomography complemented efforts to evaluate the role of measured genetic variation in predicting coronary artery disease.