6.1.1 Harvest of SVF Harvest of SVF is done under sterile conditions in the outpatient clinic. The patient is positioned supine on an examination bench. The area from which the tissue will be harvested is marked on the skin with a surgical marker. The area is app. 8cm (cranio-caudal) x 20cm (lateral) and situated just below the umbilicus. Local anesthesia is applied to the whole area with a suspension of Carbocain 1%, 200ml isotonic saline, adrenaline and 10mmol Bicarbonate. Surgical cleaning is performed twice and a sterile draping is applied. Through a stab incision the harvesting needle is introduced to the subcutaneous fat and suction is applied. 20ml to 40ml of subcutaneous adipose tissue is harvested. At the end of the procedure the skin is closed with a plaster.