By employing multiple fluorescence quantitative PCR, several target genes can be simultaneously and quantitatively amplified. Therefore multiple fluorescence quantitative PCR is more suitable for clinical application. This technology will not only provide higher accuracy, specificity and reproducibility but also simplify procedure and reduce measurement cost. The recombinant plasmids of MDR1 and WT1 gene have been constructed and the methodology of multiple fluorescence quantitative PCR has also been developed in our group .14