Blood and ascites will be analyzed for various cell populations by flow cytometry (different myeloid subsets including polymorphonuclear cells, MDSC and monocytes, T cell sub-populations including Tregs, CD8 and CD4 T cells, , B cells, and immature and mature NK cell). Myeloid infiltrate will be also evaluated by immunochemistry staining of CD68, CD163, CD206 and SIRPα of HCCs from the tumour bank.