The investigators propose to first degree relatives of CD patients with or without symptoms and to subjects with autoimmune disease tested negative for serum anti-tTG but positive for CD related HLA DQ2 or DQ8 a prospective study to uncover early-stage of gluten intolerance by measuring the mucosal VH5 restricted gene family anti-tTG clones in two biopsies: before and after one year of gluten free-diet (GFD). Symptomatic subjects genetically predisposed to gluten intolerance, tested negative for CD related autoantibodies and with apparently normal intestinal mucosa, were also enrolled for detecting the deposits of IgA transglutaminase at intestinal level and, after one year of gluten free diet, and to evaluate the clinical modifications.