In 1985, the program was enlarged to make a comparative analysis of the 12-year incidence of coronary heart disease in the Japanese and Hawaii study cohort of the original NI-HON-SAN Study, to sample 400 offspring of the study cohort ages 45 to 60 years and to complete nutrient indicators for association with blood pressure in a manner consistent with the United States-Japan study of nutrition and blood pressure. Resulting data were compared with a comparable selected group of Japanese men and with the parents of the offspring to examine familial associations. Pulmonary function data were recorded and analyzed for association with other risk factors, respiratory disease, and other major chronic diseases.