Microbiome Data Analysis The main outcome is the percent relative abundance (PRA) of microbial groups, and we hypothesize this will differ between PPI users and non-users of acid suppression medications. Specific microbial groups (species/genera/phyla) that differ between experimental groups will be identified using a non-parametric two-part statistic.40 Statistical analyses will be assessed at = 0.05 and p-values corrected for false discovery rate (FDR).41 Results will be validated by species-specific QPCR.