1. Sample Collection A minimum of two study sites will participate in this study. Approximately 218 individuals will be enrolled in this study. At least 58 collected samples must be from self-declared smokers. Oral fluid samples will be collected from individuals, who will give the informed consent and self-report in an anonymous fashion whether or not the individual is a smoker and number of cigarettes smoked per day on average. Two samples will be collected from each individual for this study. One sample will be collected using the VioOne Collection Device and one will be collected using a commercially available saliva collection cup.