In follow-up to the former study, we tested the performance effect of a carbohydrate mouth rinse 2 h after subjects ingested a CHO rich meal. From an evolutionary viewpoint, it can be speculated that the potential stimulating effect of glucose in the mouth might be of considerable impact under conditions when liver glycogen stores might be compromised. In agreement with this theory, there was no performance improvement with a CHO mouth rinse during time trial performance 2 h following the ingestion of a CHO -rich meal. However, as there are now studies demonstrating results both for and against any ergogenic effect of a CHO mouth rinse during short duration time trials it remains to be determined whether CHO mouth rinse improves performance during a 1 h time trial in the fasted state only. Therefore, the present study aims to investigate any potential ergogenic effect of a CHO mouth rinse during a 1 h time trial performance in both the fasted and fed state.