When compared, the subcutaneous and intramuscular routes were shown to produce the same responses. • The vaccine has been shown to reduce clinical signs and mortality and reduce virus shedding in infected chickens. • The vaccine has been shown reduce virus shedding in infected ducks. • The vaccine is able to induce antibodies in a wide range of birds. • If the circulating avian influenza field virus has a different N component to the N1 included in the vaccine, it may be possible to differentiate between vaccinated from infected birds by using a diagnostic test to detect Neuraminidase antibodies.