All participants were closely observed by physicians during and post-treatment. Oxygen saturation and vital signs were monitored for 30 minutes before, during, and 30 minutes after the treatment. Pulse rate was measured by automatic sphygmomanometer (Ua-767 Plus, UK) in the supine position. Blood pressure (mm Hg) was measured by automatic sphygmomanometer (Ua-767 Plus, UK) in the supine position with a pediatric-size cuff wrapped around the right upper arm. Body temperature was measured by an axillary electronic thermometer. Respiratory rate was measured by counting chest rising for 60 seconds. Pulse oximeter was placed on the left index finger to monitor oxygen saturation throughout the procedure.