Preliminary Studies The research group has also optimized Eyeprim collection of conjunctival immune cells and method of analysis using a panel of immune markers with Amnis [31], a relatively new type of flow cytometry (Figure 1) coupled with microscopy (Figure 2). The advantage of the Amnis technique is the direct visualization of analyzed cells to permit co-localisation of signals. They have published on the validity of the cell morphological parameter analysis using the Amnis software, compared to a human grader [31]. In addition, they have also used conventional flow cytometry (BD FACS) to quantify CD3, CD4, CD8, CCR7, CXCR3, CD109 and CD63 staining (data not shown).