anti-CD196 (CCR6) AlexaFluor 647 anti-CD3 APC / Cy7 anti-CD4 PE-Cy5 anti-CD161 PE / Cy7 antiCD Alexa Fluor 488-25 PE anti-FoxP3 The analysis will be performed at the Research Unit Experimental Medicine, UNAM, in the laboratory HIPAM (liver, pancreas and motility). FACSCalibur flow cytometer with DIVA software II is used. at least 10,000 10,000 regulatory T cells and Th17 be analyzed.