5. Sampling techniques: Blood samples will be taken from ante cubital vein after an overnight fast. Samples will be taken from all groups at the initiation and at the end of 3 months. Serum levels luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin, testosterone, IL-6, IL-8 and insulin will be analyzed by ELISA method. Fasting glucose will be performed by chemistry analyzer. Evaluation of insulin resistance will be performed by using HOMA-IR Index (Homeostasis model assessment for insulin resistance) with following formula. :