iv) Detachment of cells from membranes Using a 10ul micropipette tip, scrape the cells off the membrane. Ensure to scrape the correct side of the membrane (this can be done by referring to the direction of the hooks of the membrane). This is most easily done by pinning down the membrane against the wall of the vial tube and scraping it off with a grinding-like motion (the wall of the vial tube is the mortar and the micropipette tip is like a pestle). Repeat for the other membrane in the same vial tube. Also, repeat the same steps for the other eye. Once scraping of cells is completed, placed the 2 eppendorf tubes onto the ice and hand over to the Aihua or Tanima (Academia Level 12) for FACs analysis on the same day.