In collaboration with the ENA team, their team has been trained in image acquisition using CCM to enable quantification of corneal nerves and dendritic cells [1, 2, 4, 9, 10, 13, 14, 16, 21, 27](Figure 3). Sub-basal corneal nerve fibre density, corneal nerve branch density, corneal nerve fibre length and tortuosity (Figure 3, 4) will be quantified using both manual (CCMetrics) and automated (ACCMetrics) image analysis software, developed by the ENA team. Sub-basal mature and immature dendritic cell density and distribution in relation to corneal nerves will be quantified.