By using two scrubs instead of one, the percentage of organisms removed from the sample site is increased, while scrubbing the cutaneous surface appears to be a vital part of the sampling process. Even though the majority of organisms are located on or near the surface of the skin, some have adherent properties, while others are situated in deeper parts of the horny layer and hair follicles. This might explain why washing of the sample site without scrubbing is inferior to the scrub-method, only yielding under half of the organisms sampled by scrubbing (30). The efficiency of this method has been evaluated by other authors and is seen as an appropriate approach to skin sampling when the goal is to evaluate both superficial and resident/"hidden" flora.(5, 6, 31)