A 3-way ANOVA assessing the impact of ASD, age, and gender on miRNA expression will be employed along with a Mann Whitney Test. Targets with a P-value < 0.05 will be selected for further analysis after multiple testing correction with Bonferroni analysis. Significantly altered miRNAs will be queried on Mirbase.org for known mRNA targets. The target list will then be examined using DAVID bioinformatics functional annotation tool for enriched gene ontology categories. Correlations between miRNA expression and medical/neuropsychological characteristics will be calculated using Pearson Correlation statistics, filtering for miRNAs with p-value<0.05 and r>0.5.