4. Microbiological sample Microbial samples from a tooth in the same sextant as the implant and from the implant healing cap prior to its initial removal at the start of the restorative procedures will be obtained. Additional samples will be taken at 1month, 6, 12, 36 and 60 months after the insertion of the restoration. The samples will be analysed for P. gingivalis, T. forsythia (B. forsythus) T..denticola, .F. nucleatum, P. micra (P. micros) P. nigrescens, P. intermedia, C. rectus, C. gracilis, E. nodatum and S. constellatus. (Quirynen et al. 2002, Keller et al. 1998, Conrad et al. 1996). Sample analysis will be performed at the Forsyth Institute, Boston, MA, on a contractual basis. Samples will be identified only by their number for this purpose. No patient identifying data will be revealed. The correlation between the periimplant microflora before and after loading within the same subject as well as potential differences in quantity and composition of the microflora of ZCC and TCF implant abutments and restorations will be analysed.