Inguinal node metastasis in patients with squamous cell carcinoma of the vulva is reported to be as high as 27%(21). Consequently, a large number of patients may undergo a potentially morbid procedure without any gain. Non-invasive methods for the evaluation of the inguinofemoral nodes such as CT, MRI and PET scans have not yet been proven reliable. Sentinel node biopsy utilizing Tc-99m sulfur colloid and isosulfan blue dye allows for evaluation of nodal status in patients with vulvar malignancies through minimally invasive surgery. Our study, at Women and Infants' Hospital, employed Tc-99m sulfur colloid and isosulfan blue dye to identify sentinel nodes in the inguinal lymphatic chain of patients with primary squamous cell carcinoma of the vulva. Twenty nine patients were enrolled and 107 sentinel nodes were studied with no false negative SLN being reported(22) There are over 175 patients in the reported literature who have undergone a SLN dissection for the evaluation of the inguinal lymphatic basin for metastatic disease. To date there have been no false negative SLN reported. We feel the SLN dissection has been proven to be reliable and safe procedure both at our institution and others around the world. Further studies need to be performed in order to evaluate the use of SLN dissection alone in patients with pathologic negative sentinel nodes for metastasis. This study will evaluate the use of sentinel node dissection alone and management of these patients who potentially do not need or will benefit from a full inguinal node dissection.