Once the samples are finished running, the gel is removed from between the glass plates. The gel was then stained using a color staining solution. The Solution was made using 40 mg of 3,3'-5,5' tetramethyl benzidine and 20 ml of methanol. This was left to mix for 15 minutes. Next 2 ml of dimethyl sulfoxide is added and left to mix for 5 min. This was followed by the addition of 40 ml of acetic acid 5% which is made of 2 ml AA and 38 ml of DDW. Next 40 mg of potassium fericyanide dissolved in 4 ml of DDW are added. Finally 600 μl of H2O2 is added to the staining solution and left to mix for 10 min.