The sample for each patient is prepared using 10 μl of the serum, 2 μl of Hemoglobin, and 12 μl of a commercial loading buffer. The sample is then carefully placed in the prepared wells using a pipette. 4 wells are reserved for 3 controls of Hp 2-2, Hp 2-1, and Hp 1-1 followed medially by an empty well.