Data Handling & Analysis To ensure quality and integrity of the data collected case report forms will be utilized. The case report forms (CRF) will be designed to record data collected in a way that will meet the highest standards. CRF will be developed, tested and approved prior to any subject enrolment. The scientist involved in the data collection of the study will be trained on the use of CRF prior to beginning of data collection. A paper and digital library for all CRFs will be established and maintained during the experiment and at least for 2 more years after the publication of the manuscripts. The data base manager and the principal investigator will be the only person that will have access to identifiable subject information. All the rest of the documents will be coded to ensure subject anonymity. Data and Safety Monitoring Plan includes: Overall framework for data and safety monitoring, responsible party for monitoring, and procedures for reporting adverse events/unanticipated problems. Following data collection, data entry will take place by two authorized scientists. Data from Quest diagnostics are available in PDF format and data entry will be also performed and verified by two scientists. Data integration and database cleaning will be performed in statistical software via analysis and visualization.