The western blot analysis is run in a Protean II xi cell by BIO-RAD. The apparatus is assembled and an electrophoresis running buffer is added. The running buffer contains 15.1 g of Tris, 72 g of Glycine, and 1000 ml of DDW. The running buffer was then placed in the upper and lower reservoir of the Protean II. The electrophoresis was run at 240 Volts for 3 hrs.