Dukoral. The cholera vaccine, Dukoral is produced by the Swedish Bacteriological Laboratories (SBL Vaccin AB Stockholm, Sweden). This inactivated, oral cholera vaccine consists of killed bacteria prepared from individual strains of O1 ElTor and classical V. cholerae that include both LPS and protein antigens, and also contains recombinant B subunit of cholera toxin (rCTB). One dose of vaccine contains 1.25 x 1011 killed whole cells plus 1 mg of rCTB. Immediately before use, individual doses of the liquid form of the vaccine will be mixed with the reconstituted bicarbonate buffer. For children, the vaccine will be suspended in 20 ml (total 4 teaspoon) of bicarbonate-citric acid buffer [the buffer is prepared by adding 100 ml water to a sachet of bicarbonate buffer (2 g bicarbonate; 750 mg of citric acid; Recip AB, Stockholm). For adults, Dukoral will be reconstituted in 100 ml of the buffer.