Laboratory Analysis Samples will be analyzed for levels of cortisol, C-reactive proteins, and cytokines interleukin (IL)-1α, IL-1β, IL-1ra, IL-2, IL-6, IL-8, IL-10, and TNF-α, by appropriate methods, including ELISA or multiplex fluorescent bead technology. All samples will be run in duplicate on with plates and reagents of the same lot. In addition we will test Adrenocorticotropic hormone (ACTH), glucose and insulin to evaluate the response of the stress. Any samples varying greater than 15% between duplicates will be repeated.