3. Image Analysis, In-gel trypsin digestion and MALDI mass spectrometry analysis Stained gels will be scanned with GS-800 densitometer (Bio-Rad) using PDQuest software (Bio-Rad) and the proteins of interest will be analyzed by the MALDI-TOFMS (Voyager DE-PRO, Applied Biosystems) at Institute of microbial technology (IMTech).