During this visit a complete clinical history will be elaborated and data about antecedents of leishmaniasis obtained (administered treatment, localization, number of lesions, etc). A full medical evaluation will be realized based on universally accepted techniques. The inclusion/exclusion criteria will be applied and the selected candidates informed about the study after which they will sign an informed consent. For those subjects with ulcers of more than two weeks of evolution without parasitological diagnosis a direct test will be performed by the parasitologist. If the first direct test is negative, it will be repeated up to three times; after which, in case of negative results, a biopsy will be performed. In case leishmaniasis is not confirmed the patient will be remitted to the original health center.