All data will be analysed using SAS version 8.2 (SAS Institute Cary, NC, USA). Data will be assessed for normality and log-transformed where appropriate. Univariate analysis will be conducted using chi-square test for equal proportion, analysis of variance and non-parametric Kruskal wallis tests where required. Multivariate analysis will be performed using generalised linear modelling adjusting for potential covariates and repeat measures. A two sided p-value of 0.05 will be considered to be statistically significant.