Samples Analysis A total of fourteen blood samples will be collected in each experimental trial and analyzed for: hematocrit (Hct), hemoglobin (Hb), osmolality (Osm), sodium (Na) and potassium (K), total plasma protein, glucose, insulin, c-peptide, glucagon, copeptin, plasma renin activity, corticotropin releasing hormone (CRF), cortisol, triglycerides and free fatty acids (FFA).