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Gbakker, here is your draw:
There were 28 items in your list. Here they are in random order:
Posted by gbakker on 3/16/2013 6:57:00 PM (view original):I assume that if I have the Braves that doesn't count the Beaneaters?
Yes, it does. Check baseball-reference.com for history of all franchises. You have all teams in your franchise history. Remember, no AL franchise has a history before 1901.
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Astute students of history have noted, weather is not something politicians should take lightly. The presidency of William Henry Harrison, indeed Harrison's life lasted a mere month after after he caught a cold at his inauguration, which was held outside on a chilly Washington morning.
Drudge has a cool photo:
Then there's this:
As the rain began to pour harder, Obama noticed his running mate's stool close to the edge of the slippery stage.
"I'm gonna ask Joe to move that stool up because I don't want to have to choose another vice-president," Obama joked. "I don't want him slipping over, toppling over there."
That's not what I heard.

subset: commoncrawl


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Being a freshman in high school is a big adjustment for any student.
Being a freshman wrestling on the varsity? That only ramps up the heat even more. Just ask Cary-Grove sophomore standout Mike Cullen.
"Being a freshman last year I remember going out there and having real bad anxiety," he says. "I just wanted to go out there and be done with it and just get by each match. As a freshman, you aren't as mature. It's tough when you are wrestling kids who are older than you and more mature and tougher than you. I won two IKWF (kids) state titles. Nothing quite compares to the high school atmosphere. I was kind of a big dog in IKWF, but I got to high school and it was a different story. I didn't do bad last year, but it was a lot tougher. I learned real quick I was no longer the top dog."
Age and confidence differences aside, Cullen handled himself quite well as a ninth-grader, finishing with a 44-4 mark, a Fox Valley Conference title and a trip to the state tournament.
And the 113-pounder has picked up right where he left off. Cullen, now a two-time FVC champion, won his first regional title last week and brings a perfect 39-0 record into this weekend's Class 3A Barrington sectional. He's now 83-4 in his brief high school career.
"Honestly, my technique is all the same, but I have a mental edge now," says Cullen, whose younger twin brothers, John, and Sean, are on the team as well (both compete at 106 pounds; John Cullen also qualified for sectionals). "I'm not a freshman anymore. Being a sophomore, I have a little more of that mental edge on people. I'm a little more confident when I go out there. I'm not as nervous and anxious. Last year I felt like a freshman and an underdog a lot of the time. I would get super uptight. I wasn't used to the high school wrestling experience. Now I feel more comfortable and when I feel more comfortable, I'm able to open up and be more offensive and work all my stuff."
Cary-Grove coach Ryan Ludwig has seen a transformation in Cullen this season.
"Mike came in as a freshman as an extremely strong wrestler," Ludwig said. "He's really matured this year. His training always has been good. He's a lot more focused now on and off the mat. He had a lot of big goals last year and got Downstate for the first time. That's a big thing to get down there and wrestle in front of everybody. This year, he's even more prepared for it."
Cullen also credits the extensive work he has put in over the years at clubs such as The Wrestling Factory and Overtime for helping prepare him to wrestle in high school.
"I've trained with some of the best clubs," he said. "I have to give both clubs a lot of credit for being able to come in as a freshman and do as well as I did."
Ludwig notes coaching a wrestler of Cullen's experience and talent is a tad on the different side in terms of approach.
"He came to us with a lot of experience," the coach said. "He's extremely impressive. It's exciting and fun to have the opportunity to coach him. We're always looking for the little things that can help him even further. The last two years we've been able to give him those things that have helped him get ready for what's ahead in two weeks."
Cullen has been wrestling since the age of four and comes from a wrestling-centric family. In addition to his twin brothers being in the room with him, Cullen's older brother, Brian, was a three-time state qualifier (finishing second at 103 pounds for Prairie Ridge in the 2010 Class 2A tournament) and is now wrestling at the University of Illinois. Cullen's father, Michael, wrestled at Palatine High School and was a two-time state qualifier.
"It's fun being with my brothers," Cullen says. "We get to spend more time together and at the same time we get to go at it in the room. We're a tight family. It's in our blood."
Ludwig loves the fact the three brothers are together in the training room, frequently working with each other.
"It's been a pretty special season because of that," Ludwig said. "The three of them work out a lot together. Michael takes his brothers under his wing and works on stuff with them. He has extremely high expectations for his brothers. It's real cool to see how close they are. It's fun getting to know the three of them. They all have different personalities and react different to coaching. They push each other and work extremely hard when they practice together."
John Cullen brings a 31-10 mark into the 106-pound division at Barrington. He placed third at the regional to qualify.
"I've been pushing myself a lot in the room with my brother," he said. "Mike likes to push me harder and harder to get better. It's been hard, but you get trough it. My brother has been a big part of my improvement. Just wrestling with one of the best people in the state is fun in it's own way. I'm getting one of the best looks of anybody. I'm not going to see anybody that is better."
Improved footwork has aided John Cullen as the season has gone on.
"I work on taking shots and not letting people get angles on me," he said. "My off-season work has helped me. I'm not just working during the high school season. You have to work hard all year."
John Cullen also enjoys the family's wrestling pedigree.
"Everybody in the family knows how it is to wrestle," he said. "We all come to practice and do the same thing and everybody relates to each other. Even my dad wrestled. He's the one that got me into it."
John Cullen knows he will be facing a tough sectional field this weekend, but is more than prepared for it.
"I just have to keep motivated and focused and not let anything get to my head," he said. "I can't look too far ahead. I have to keep a clean thought and never doubt myself. You never really lose until you stop trying."
Ludwig feels John Cullen has a good shot to make a trip to Champaign as a competitor next week.
"I absolutely think he does," Ludwig said. "He's a very talented freshman who does a lot of great things. He has been exposed to a lot of big matches over his career and knows what he needed to do to move on."
As far as big brother goes, two of Mike Cullen's losses in his high school career occurred last year in the state tournament. He says another thing that's aided him this season is having a defined road map in terms of what he would ultimately like to accomplish.
"I've set my expectations extremely high," he said. "Throughout the season it's a steppingstone to get to that major goal, which is to win a state title. I go out there with that attitude in my matches. I know if I go out there relaxed and I keep working my stuff, that will help me get to that next level eventually."
Mike Cullen has been attending the state wrestling finals in Champaign at Assembly Hall since he was 6. He'd like to get back to Champaign and work toward what he witnessed so much as a child -- getting on that first-place medal stand.
"I watched some of the best guys in our generation who are competing in college now," Cullen said. "I idolize those guys. It's always been in the back of my mind. It's what it is all about. It's something I have been striving for since I went down there the first time. "


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WASHINGTON, April 12 -- A group of moderate House members known as the New Democrat Coalition argues that comprehensive immigration reform would create more jobs and make the country more competitive in the global market.
"Our history has shown that immigrants and their children start businesses and create hundreds of thousands of jobs that make America great," said Democratic Rep. Joe Garcia of Florida in announcing the plan at a Capitol Hill news conference on Thursday.
A bipartisan group of eight senators is likely to unveil its plan for immigration reform next week.
That plan is widely expected to be the starting point as both chambers of Congress hammer out a bill that would address the needs of the estimated 11 million people who are in the U.S. illegally while also allowing new immigrants to gain permanent residency.
The coalition's plan, while calling for more work visas to help spur entrepreneurship in the global economy, advocates strongly for the resources needed to secure U.S borders in a way that provides for a fluid exchange of trade.
"While we are committed to having real enforcement as part of immigration reform, we want to make sure the undue burdens don't hammer economic growth, but in fact enhance [it]," said Rep. Jared Polis, D-Colo. This is the first time the coalition, which started in 1997 and is composed of 50 House members, is taking a stand on immigration as a caucus.
The coalition has seven task forces that focus on issues such as immigration, education, national security and energy. In addition to Garcia and Polis, the other chair of the immigration task force is Rep. Joaquin Castro, D-Texas.
"We're excited about the immigration debate that is taking place right now in Washington and are glad to be a part of the discussion," Castro said.
The coalition's plan, aimed at influencing the final legislation, makes the case that border protection will become less of an issue as more immigrants are granted citizenship.
"I think once everyone becomes legalized here, we won't have the great need of border enforcement that people think they need right now," said Rep. Juan Vargas, D-Calif.(Video By Brina Monterroza, Medill News Service)


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SAG-AFTRA Houston-Austin Happy Hour on Tuesday
02/20/2013 1:30am
Come on by and meet your fellow SAG-AFTRA members over a glass of wine, a cold beer, coffee or a cocktail. Live music starts at 7 p.m. No meeting, just greeting!
When: 5:30 - 7:30 p.m. (and beyond, if you wish!), Tuesday, Feb. 19, 2013
Where: Hefley's (Inner Loop)
138 West Gray
Houston, TX 77019
(near downtown)
Cash bar with happy hour prices until 7 p.m.


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Norman Schrolucke, 90
Norman Henry Schrolucke, 90, formerly of 10472 Plattner Pike, New Knoxville, died Wednesday evening, July 27, 2011, at the St. Marys Living Center.
He was born Feb. 7, 1921, in Van Buren Township, Shelby County, the son of Louise (Henschen) and Henry Schrolucke. On July 4, 1943, he married LaRue Eversman on July and she preceded him in death Feb. 19, 1990.
He was preceded in death by two daughters, Beth Thomas and Judy Grimes.
Services are at 1 p.m. Saturday at the First United Church of Christ in New Knoxville, with the Rev. Dr. David Williams officiating. Burial will follow at Pilger Ruhe Cemetery.
Friends may call from 10 a.m. to 1 p.m. Saturday at the church.
Donations may be given to Grand Lake Hospice or the Auglaize County Cancer Association.
Condolences may be sent to the family at Millerfuneralhomes.net.
Vornholt-Miller Funeral Home in New Knoxville is in charge of the arrangements.


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I live in Egypt on the Red Sea coast where water is rare.
I am trying to get a project together to supply a camp where I work with desalinated water.
The project is to get around 15 tons of water a day that will be balanced and safe for drinking.
Previous experiance I have from desalinated water always gave us brown rusty watey from steel pipes because the water was "aggresive".
Recently, I have been told that you can add what is called a calcite filter but don't know if I could realistically use it for that much water and what quality I would end up with.
What do you think?


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Dr Stanley Ho Gr of OIH, Chev Leg
Dr Stanley Ho is a leading businessman in Hong Kong and Macau - Group Executive Chairman of ShunTak Holdings Limited and Managing Director of Sociedade de Turismo e Diversoes de Macau, SARL. His business interests span the world, covering tourism and entertainment, shipping, property development, banking, airport and airline, and many others.
Among his many concerns, his interest in ballroom dancing is well known. He is a generous sponsor of major dancing performances in Hong Kong and Macau, like the Hong Kong Arts Festival and the Macau Arts Festival, and has been actively involved in promoting dancing by inviting internationally renowned dancing groups to perform in Hong Kong and Macau, like the National Ballet of China.
He is a keen supporter of the Royal Opera House Trust and an Honorary Member of the Grand Council of the Imperial Society of Teachers of Dancing. Dr. Ho is noted for leading the dance floor in performances of the Tango, Cha Cha and Waltz in important charitable functions, and is often seen on television for dancing performances to raise funds for the charities.
Grand council: Grand Council Member


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LOS ANGELES – The Dodgers Dream Foundation, in partnership with the LA84 Foundation and City of Los Angeles Department of Recreation and Parks, unveiled a new Dodgers Dreamfield today at Reseda Park in Reseda. Those on hand for the dedication ceremony included President and CEO, Los Angeles Dodgers, Stan Kasten, General Manager, City of Los Angeles Department of Recreation & Parks, Jon Kirk Mukri, Councilmember Dennis Zine, District 3 and President, LA84 Foundation, Anita L. DeFrantz. Dodger broadcaster Charley Steiner hosted the event.
Dodger alumni Al "The Bull" Ferrara, Lee Lacy, Ramon Martinez, Fernando Valenzuela and Steve Yeager and Dodger prospects Onelki Garcia, Matt Magill and Joc Pederson were in attendance and also led a clinic on the new field following the dedication.
"We are proud to continue one of the Dodgers Dream Foundation's cornerstone programs – Dodgers Dreamfields," said Los Angeles Dodgers Owner and Foundation Chair Mark Walter. "Today's dedication at Reseda Park is one example of our commitment to Los Angeles youth. We look forward to continuing to provide athletic, educational and health-related opportunities for the greater Los Angeles community."
"The LA84 Foundation is pleased to have partnered with the Dodgers Dream Foundation and the City of Los Angeles Recreation and Parks to bring a state-of-the art baseball field to Reseda Park," said DeFrantz. "Boys and girls in the Reseda Park community will now be able to hone their baseball skills and dream of someday being in the big leagues each time they step on the field especially improved for them. We look forward to continuing the great partnership with the Dodgers Dread Foundation to bring more fields to other communities in Los Angeles."
"I am very pleased that the Department of Recreation and Parks has again partnered with the Dodgers Dream Foundation and the LA84 Foundation in 2013 to add even more Dodger Dreamfields throughout the city," said Mukri. "These re-built and renovated fields have been a great success bringing a myriad of upgrades that promote youth sports and an active lifestyle. The support of both the Dodgers Dream Foundation and LA84 Foundation helps the Department continue to build healthy communities through people, parks, and places."
"I am elated that the community of Reseda was chosen to be the site of the next Dodger Dreamfield," said Zine. "The goal of the Dodger Dreams Foundation to bring educational, athletic, and recreational opportunities to the youth of the Greater Los Angeles area is truly admirable. I support their mission wholeheartedly and this foundation is making lots of dreams come true in Reseda today."
Field improvements at Reseda Park's Dodgers Dreamfield include new gauge chain link on the backstop and outfield fence lines, newly painted posts and rails, renovated infield laser grading, new infield mix, new sod, newly newly installed bases, a 7' wide warning track, renovated irrigation system and irrigation clock and a new solar powered scoreboard and signage.
About the Dodgers Dream Foundation
Founded in 1998, the Dodgers Dream Foundation (DDF) was created to provide educational, athletic and recreational opportunities for the youth of the Greater Los Angeles community. The Foundation places special emphasis on helping traditionally under-served youth. The Dodgers' Dreamfields program is the Foundation's signature initiative. The program is a partnership between the Dodgers Dream Foundation, the City of Los Angeles Department of Recreation and Parks, LA84 Foundation and local city officials. For more information about the Dodgers Dream Foundation, please visit http://www.dodgers.com/community.
About LA84 Foundation
The LA84 Foundation was established to manage Southern California's share of the surplus from the highly successful 1984 Olympic Games in Los Angeles. The Foundation received $93 million at its inception. Since it began operations in 1985, it has invested more than $207 million in sports programs serving more about three million youth in the eight Southern California counties of Los Angeles, Imperial, Orange, Riverside, San Bernardino, San Diego, Santa Barbara and Ventura. Its headquarters is located in the historic Britt House near downtown Los Angeles where it houses the world's premier sports library and meeting facilities. The Foundation provides grants to youth sports organizations, manages programs, including a coaching education program, and convenes numerous forums for the exploration of the most pressing issues in sport. For additional information, please visit http://www.LA84Foundation.org.
About the City of Los Angeles Recreation and Parks Department
The Department of Recreation and Parks maintains over 15,710 acres of parkland with over 400 neighborhood and regional parks, 184 recreation centers and facilities, 372 children's play areas, 321 tennis courts, 256 ball fields, 31 senior centers, 13 golf courses, nine dog parks, 14 skate parks, 12 museums and an urban forest of one million trees. The Department also provides after school and day care programs for children teens, including basketball, volleyball, softball and flag football leagues. For more information about the Department of Recreation and Parks, please visit http://www.laparks.org.


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The quadratic function f(x)= px^2 + qx + r has f(0)= 35,f(1)=20 and f(2)=11.
Find the values of the constants p,q,r.
Express f(x) in the form a(x+b)^2+c. Use your answer to find the smallest value of f(x).
The only thing I know is r=35 (I think)
please help
thank you


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I usually can finish most of the shows I start watching. I did drop Gunslinger Girl doe, I know you can't judge a anime by the first episode, but after like the first 5 minutes that show put me to sleep.
I kinda stop watching Naruto and Bleach, only watch the Anime for the fights.
D. Gray man
Eyeshield 21
Eyeshield 21
^ Those are just the ones that are in my mind at the moment.
[HIATUS] oops.
Ranma 1/2 - made it to season five just out of stubbornness, but really, after season two, what's the point?
Magical Lyrical Girl Nanoha - the transformation sequences were way too close to kiddie porn for my tastes.
Hamtaro - the voices made my teeth hurt.
World God Only Knows - *quietly bangs head against wall*
Cautiously optimistic...
Cardfight!! Vanguard
This was the stupidest anime that I saw
I bet Precure is better then this
"No matter how fast it flies, a paper airplane cannot break through a brick wall
I've dropped Bleach, Prince of Tennis,MAR,Blue Dragon, Air Master,Chrome Shelled Regios,Kite,Mouse,School Rumble,Honey and Clover,Slam Dunk,Eureka Seven,Hanasaku Iroha,Yamada's First Time,Level E,You and Me,Nurarihyon no Mago and Shakugan No Shana. All those animes i started to watch but i stopped watching them because i guess i just got bored with them. Oh and i forget Ikkitousen,Queen's Blade and Pandora Hearts. Pandora Hearts is SO GOD DAM SAD
Feeling like a straight up gangster today
lol @ your userpic then...
Quezz wrote:
I usually can finish most of the shows I start watching. I did drop Gunslinger Girl doe, I know you can't judge a anime by the first episode, but after like the first 5 minutes that show put me to sleep.
i'd recommend try again, then, and giving it at least the first full ep.
unless the entire concept of "cute girls cyborged up as government assassins with fun military weaponry" totally bores you off in the first place.
SmudgeAnim wrote:
Ranma 1/2 - made it to season five just out of stubbornness, but really, after season two, what's the point?
Magical Lyrical Girl Nanoha - the transformation sequences were way too close to kiddie porn for my tastes.
Ranma: omg, no kidding, right? i love the characters, but really.. too many seasons to go on for -no- character development at all... (powerups are NOT character development, no matter how cool they are.)
Nanoha; heh. you haven't really watched many magical-girl type shows at all, have you? i'm not exactly saying i -disagree- with what you're saying... but it's pretty much par for the course. also, just hit the "skip 30 seconds" button, and they're probably done with it. the rest of the story is really good, and (unlike most magical girl shows which seem to depend heavily on re-using stock footage) they cut back a lot on the amount of full-length transforms, except for like climactic battles.
... again.. not like the nanoha series doesn't have its flaws.. but as the genre goes it's realyl one of the best examples i've seen. and considering that the character IS in fact a spin-off from an H-game.. heh.
for me, i tend to just drag myself thru most series, and enjoy them for what they are. sometimes they might be pretty boring, but i still finish them. only one series -really- stands out as making me regret the time i've invested in it. (even ranma 1/2, despite repetitiveness is still lots of fun if you watch in small bits at a time)
*dons fire resistant suit and heavy armor*
Full Metal Panic: Fumoffu.
*leaps behind cover*
but seriously.. i loved the mix of drama and comedy of the first two series.. (FMP, FMP:2nd raid)
and then in Fumoffu, they completely throw out any pretense of seriousness, and he spends -several- episodes running around in a brown mouse suit. i kept hoping (pleading, praying) that they were just starting out light, and the real storyline is going to pick up any episode now. and then i ran out of episodes. geh. give me my 6 hours back >.<
--add: Samurai Shodown the anime is really horrible too. but it's MST3K horrible, so it's still fun to watch just -because- it's so terrible.
Anything with a big jugs I mean to much. And pretty much all the mainstream shot I do not watch.. I guess I could calling it a drop since I watch one episode or two. Find out its crap. Most anime I do watch. Even some badly rated ones. I'm not picky when comes to most anime.
I recently watched Happiness! and my god it was HORRIBLE. There was practically no plot and throughout the whole anime, I kept asking myself, "when is the good part going to come in??"
Fate/stay night: Heaven's Feel
Let's see, what have I dropped...
Naruto: It's just fatigue really, combined with a period of time not having access to it. I still keep up with the manga, but the episode # is so large that i just don't feel like keeping up at this point =P
Shugo Chara: I dropped it off at like 10 episodes left cause I was watching it mostly due to joining a friend's RP based on it. I should finish it...or at least the first series. Not sure about anything past that.
Actually I've done that to a lot of series. Digimon Savers I stopped with a few episodes to go XD, or Full Metal Alchemist Brotherhood. Very odd. I blame real life for the most part. I can't think of a lot of series I dropped just cause I didn't like them. I don't count watching one episode and then nothing else as 'dropping' it so that probably lowers the #.
OMG! I almost dropped D. Gray-Man but I toughed it out to episode 51 and then the series' excitement level shot through the roof. It turned out to be a really cool series well worth watching. Glad I finished it.
Anyway, I usually always try to finish the anime I watch but some I just couldn't and some I am still working on.
I watched the original Mobile Suit Gundam, then dropped Mobile Suit Zeta Gundam about half-way through.
Hajime no Ippo was to much of a sausage fest for me. I mean seriously, the only girl I saw after the first 9 episodes was his mom.
I tried to finish Ranma 1/2 but at about half-way through it started to feel like filler.
WARNING: the following are very popular animes that I yet to finish because they bore me. This list will probably result in me getting punched, shot or stabbed:
Golden Boy - Stopped after episode 3. Afterwards I was like "Dang! Can't watch this with my family."
Great Teacher Onizuka - Stopped after episode 1. First episode seemed like a copy of episode 2 of Golden Boy. Soon after I came to the conclusion that I really don't care much for stories that revolve entirely around only one person.
Cowboy Bebop - Stopped after episode 11 or 12. Whenever Vicious came back in, I thought things were going to get exciting but I had already lost interest. I think I am planning on buying it to finish it up but only if someone tells me that it gets better.
Samurai Champloo - Stopped after episode 13. The overall story was so freaking slow.
Shugo Chara! - Stopped after episode 9. Story began to really slow down.
By all means, if anybody can convince me to finish any of these animes, please do so.
Excited? You should be...
Soul Eater- I got about half way through and lost interest... I guess I just had better things to watch... I might finish it someday.
Only DEATH can cure STUPIDITY!!
-jigoku shojo
-Appleseed XIII
-fairy tail
-kiniro no corda
-star driver
-Asobi ni Iku yo
-Rio: Rainbow Gate
-toaru majutsu no index
Super Robot Wars OG The Inspector
En Español, las chicas llamanme "El Tigre Negro Chino Cabrónisimo". "LÀWL"
man, its painful for me to read that people dropped shows like wolf's rain and hagurasho no...(when they cry). I just think that u should give them a chance, they might be slow paced but they're worth it.
also mahou sensei gets much better with the OVAs, recommend it if u liked love hina


text:
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text:
USD Softball's McElvain Gives Gift of Life
Tuesday, January 7, 2014
U-T San Diego -- Dana Findley's children asked their mother if she'd take them to the park and play. Dana wanted to round up the toys – Jalen's soccer ball and basketball, Jade's sidewalk chalk and jump rope – load the kids in the minivan and drive to the park.
But her body rebelled, saying no. Kidney failure had left her exhausted.
Soon, the kids stopped asking their mother to play.
"Let me guess," said Jalen, "you're tired again."
On Sept. 9, Findley, 40, who lives in Paradise Hills, underwent a kidney transplant. The donor was her longtime friend, USD softball coach Melissa McElvain, 36. (Full Story)
Contact Information
Degheri Alumni Center 228
5998 Alcalá Park
San Diego, CA 92110
Phone: (619) 260-4724
Fax: (619) 260-4790


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Mark Hughes emerged from a barrage of questions about his future on Friday to insist he is still the right man to turn things around at QPR.
Hughes' men will try to secure their first Barclays Premier League win of the season at the 12th attempt on Saturday when they welcome Southampton to Loftus Road. The game against Saints has been dubbed "El Sackio" given the current predicament of both managers.
Tony Fernandes claimed on Twitter last week that QPR "must win" the six-pointer against the south-coast club, who are one point above rock-bottom Rangers. But Hughes is adamant that he can drag the west Londoners away from the foot of the table. "My future doesn't hinge on this match," Hughes told a press conference.
Copyright (c) PA Sport 2012, All Rights Reserved.
Do YOU want to write for GiveMeSport? Get started today by signing-up and submitting an article HERE: https://www.givemesport.com/writeforgms


text:
Browse by Medical Category
Accepting New Patients
Dr. Siliski focuses on all knee conditions, including arthritis, and fractures of the hip. He is actively involved in teaching medical students, residents and fellows.
Go To Programs
Note: This provider may accept more insurance plans than shown; please call the practice to find out if your plan is accepted.
Dr. John Siliski is a board certified orthopaedic surgeon. He earned his BA from Harvard College in 1973 and his MD from Harvard Medical School in 1977. He completed his residency at the Harvard Combined Orthopaedic Residency Program. Following his residency, Dr. Siliski completed his first fellowship at the Brigham and Women's Hospital in Orthopaedic Surgery and a second AO Fellowship in Europe.
Dr. Siliski treats fractures and arthritic conditions of the hip. He also treats all knee conditions and performs joint replacement, arthroscopy, ligament repair and reconstruction, fracture management, osteotomies, and patellofemoral realignment.
Dr. Siliski's research activities are focused on the clinical study of lower extremity joint surgery and outcomes. Currently, he is working on several studies related to joint replacement.
Select Publications:
Plancher KD, Siliski J. Long-term functional results and complications in patients with knee dislocations. J Knee Surg. 2008 Oct;21(4):261-8.
Lonner JH, Siliski JM, Della Valle C, DiCesare P, Lotke PA. Role of knee aspiration after resection of the infected total knee arthroplasty. Am J Orthop (Belle Mead NJ). 2001 Apr;30(4):305-9.
Gittell JH, Fairfield KM, Bierbaum B, Head W, Jackson R, Kelly M, Laskin R, Lipson S, Siliski J, Thornhill T, Zuckerman J. Impact of relational coordination on quality of care, postoperative pain and functioning, and length of stay: a nine-hospital study of surgical patients. Med Care. 2000 Aug;38(8):807-19.
Lonner JH, Dupuy DE, Siliski JM. Comparison of magnetic resonance imaging with operative findings in acute traumatic dislocations of the adult knee. J Orthop Trauma. 2000 Mar-Apr;14(3):183-6.
Lonner JH, Siliski JM, Lotke PA. Simultaneous femoral osteotomy and total knee arthroplasty for treatment of osteoarthritis associated with severe extra-articular deformity. J Bone Joint Surg Am. 2000 Mar;82(3):342-8.
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Even Pats fans can't stand Pats fans... that's why they're here.
But for real, some of you guys take this sh!t way too seriously. It's just a game.
I'd pick him up. Make the room for a player like this, really really good RT esp in a ZBS scheme (with the Texans he was a top 5 RT).
If the Jets want to solidify their Oline for the next 4 year rebuild, they would target 2 positions in FA this year.
That way they take Dion Jordan at the #1
Imagine they do trade Revis for a 1st & 2nd.
They could conceivably get Jordan, Vaccaro, Cooper, Giovanni, & go all skill position offense for the rest of the draft!
Would anyone like that?
Cople- Ellis- Wilkerson
Jordan- Harris- Davis- FA
Cro- Vaccaro- *Landry- Wilson
Dbrick- Cooper - Mangold- Howard- Winston
Powell- McKnight- Bernard
* that's a damn good start in the 1st year of rebuild
* Landry signs after not getting the ridiculous amount he was looking for.
* if Idzik gets lucky in rounds 3-7 it could be one of the best drafts ever for our Jets.
Last edited by jetster; 03-07-2013 at 07:22 AM.
Winston's a top tier RT.
We should have signed him last year, and we can't afford him this year, especially if we're going to give a second round tender to Austin Howard.
He and Houston re-uniting would make a ton of sense.
Calling him "a bust G" just makes it clear you don't have the slightest clue what you're talking about with respect to this player.
BTW, I'm sure Albert will play RT very happily if the Chiefs sign him to a long term deal at a LT salary level. Only reason he'd be upset playing RT is how it would impact his negotiating position next offseason. Money solves many problems.
The reality is, if the Chiefs draft Joeckel, they are planning to move on from Albert after this year.
Best we can do is make the irritable c*nts' stay here as unpleasant as possible.


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Six Awarded Prizes in Public Speaking Competition
Six prizes were awarded across three categories in the annual Public Speaking Competition on Saturday, March 3, in the Chapel. The finalists were chosen after an open preliminary round held in February. Speakers' presentations were either persuasive or informative in nature, and in one category students were asked to address an assigned topic.
In the McKinney Prize competition, a prize is awarded to one student from each class for a 5-8 minute persuasive speech that is socially relevant and of interest to the extended Hamilton College community.
The 2013 McKinney Prize recipients and their topics are:
Class of 2013: Mia Falzarano, "Reevaluating Summer Opportunities"
Class of 2014: Daniel Lichtenauer, "Barking Mad"
Class of 2015: Jack Boyle, "Our Abysmal Attitude toward American History and How We Can Change It"
Class of 2016: Newton Hunter Green, "Solitary Confinement: An American Epidemic"
The Clark Prize competition is open to all members of the senior class and calls for participants to address an assigned topic through both written and oral presentations. The 2013 Clark Prize recipient is Evan Van Tassell '13 on the topic "The Problem of Science in the Interstellar Age."
The final prize category, the Warren Wright prize in informative speaking, is open to those students who have enrolled in the college's public speaking course. Participants also respond to questions from the audience, and their responses are included in the judges' evaluations. The Warren Wright Prize recipient is Sandhya Rao, 2015 whose topic "Facebook: The New Big Brother."
Prizes will be awarded to all winners on Class and Charter Day.
The Public Speaking Competition was organized by the Oral Communication Center.


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WOODSTOCK – Twice a week, yellow Labrador Alex walks into what looks like a glass box without a top.
It fills with water up to about his chest, and then the floor starts moving.
It's an underwater treadmill for dogs, and Alex likes it, owner Whitney Rupp said. As do her other three yellow Labs who have used the equipment.
For about seven years, Companion Animal Specialty and Emergency Hospital in Crystal Lake has had the treadmill that Rupp and her dogs use. Veterinarian Karen Turner said there has been increasing interest in water therapy for dogs, called canine hydrotherapy.
"The underwater treadmill functions as a low-impact workout," Turner said. "It can increase muscle strength and endurance, and allows for improved range of motion in all the joints."
According to the American Veterinary Medical Association, rehabilitation for pets has emerged over the past decade from a "boutique service" to a mainstream option. Physical therapy can mean pain relief, increased mobility, and an improved quality of life.
Alex the yellow Lab began using an underwater treadmill after tearing his rotator cuffs doing agility, Rupp said. In 2010, Rupp had been living in Baltimore, and the veterinarian there recommended the post-surgery treatment.
"The weightlessness of the water takes the pressure off the joints, and that's just what he needed for his shoulders," Rupp said.
When Rupp moved to Bull Valley, she sought out a practice that had a treadmill and now takes Alex to Companion Animal Specialty and Emergency Hospital.
But the hydrotherapy isn't just for rehab; it can help with overweight dogs or to just help keep dogs healthy and in shape.
In addition to 9-year-old Alex, Rupp has three other yellow Labs – Abby, Remi and Warden – who she brings in to use the treadmill during the winter. When they come home, they're tired from the workout, which is especially nice to accomplish without having to go outside when the temperatures drop, Rupp said.
"I'm pretty toward the extreme when it comes to keeping my dogs in shape because I do competitive sports with them," Rupp said. "But … it means keeping them healthy. It means a longer life, and the risk of injury is less."
The underwater treadmill is a steep investment for a veterinary practice, let alone an individual pet owner: The price range for the equipment is between about $30,000 to $60,000.
Tudor Treadmills is a UK-based company with a location in Texas that sells the equipment. Veterinarians, universities, dog day cares and some individuals are customers, said Joanne Lisowski, who work in inside sales.
"I believe that people are treating their animals as well as they treat themselves," Lisowski said. "Some people I know will send their animals to the doctor before they go. They're demanding – they're not asking, they're demanding – the best and they really like the holistic approach."
Individual sessions at veterinary practices run in the $35 to $40 range, which Rupp said is worthwhile.
"If you think about the cost of knee surgery on a dog, in my opinion, it's a small price to pay to know your dog is in the best shape possible and the odds of them getting hurt are less," she said.
Woodstock Veterinary Clinic on Lake Avenue has had an underwater treadmill since January, veterinarian Patti Klein Manke said. Ten to 20 clients have used it since then, and she's trying to get the word out that the service is available.
The practice charges $35 for per session, each of which is about half an hour, and plan to offer a punch card as a special deal.
"With human medicine, if you have an injury, you go to rehab and it's just automatic," Klein Manke said. "Now, pet medicine has evolved so much that just about anything a person can have, a pet can have. There are knee replacements in dogs, and there have been hip replacements for a long time."
Turner said many dogs are nervous their first time using the equipment and seem bit anxious when the treadmill starts, but figure it out in a few minutes and do fine from there on out.
"Some dogs absolutely love the treadmill and they can't get in the room fast enough," she said.
Woodstock Veterinary Clinic
691 Lake Avenue, Woodstock
Underwater treadmill is $35 per session, each lasting about half an hour.
Companion Animal Specialty and Emergency Hospital
1095 Pingree Road, Suite 120, Crystal Lake
Underwater treadmill is $40 per session or $360 for a package of 10.


text: Zuffa has done a deal with Monster, the company behind Dr. Dre Beats headphones and are coming out with UFC headphones. Does it get to a point where putting your brand name on any product is too much? Especially a product that really has nothing to do with the business you're in. We're all serious UFC/MMA fans, would you buy a pair of headphones just because they have a UFC logo on them as opposed to another pair? I wouldn't and actually think its kind of cheesy. What's next, UFC oven mitts?!? Contrarily, in other Zuffa news, they have bought LA Boxing and will be converting all of those locations into UFC gyms. Now that makes sense and has some business synergy.


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David Hall: Though the mintage of the 1962 is less than one-third that of the 1962-D, the 1962 about twice as common as the 1962-D in Gem condition. The lower mintage 1962 issue was saved while the huge mintage 1962-D was not. And the 1962-D seems to have much more trouble, as do most late D mints, with rough surfaces, i.e. marks and abraisions.
© 1999-2018 Collectors Universe NASDAQ: CLCT


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Russia's president is also being criticized by Ukrainian-Americans watching the violent confrontations going on in their home country. Ukrainians in the U.S. tend to side with those protesting in Kiev's independent square. They're angry that President Viktor Yanukovych chose a closer relationship with Russia over a deal with the EU.
The Philadelphia area is home to more than 55,000 people of Ukrainian ancestry. NPR's Jeff Brady reports the community is holding rallies and lobbying their members of Congress.
JEFF BRADY, BYLINE: Outside Philadelphia in front of an old elementary school that's been converted into Ukrainian community center, about five dozen people waived blue and gold flags last night and sang Ukraine's national anthem.
UNIDENTIFIED GROUP: (Singing in foreign language)
BRADY: As the song ends, a man shouts glory to Ukraine, and the crowd responds glory to the heroes.
UNIDENTIFIED GROUP: (Speaking in foreign language)
BRADY: Some at this rally were born in Ukraine. Others trace their ties through parents and grandparents. Christina Chorpeda(ph) says her family left when she was a toddler, but she still visits Ukraine often.
CHRISTINA CHORPEDA: I cannot understand what Yannukovych is doing to my country.
BRADY: Many here echo reports that political corruption has worsened under President Viktor Yannukovych. Osa Baroshka(ph) held the corner of a banner that read simply, sanctions, asking for economic sanctions to be levied against Ukraine's leaders, including Yannukovych.
OSA BAROSHKA: He is an anti-democratic tyrant who listens to the commands of Putin.
BRADY: Russian President Vladimir Putin is another name mentioned frequently at this rally. The protest in Kiev started in November after Yannukovych rejected a pending treaty with the European Union in favor of deeper financial ties to Russia. The vast majority of Ukrainian-Americans want the country to move westward and be more independent of Russia, says Princeton University professor of politics Mark Beissinger.
MARK BEISSINGER: Many of them come from the western portion of Ukraine that is associated with a pro-EU position.
BRADY: Beissinger says Ukrainian-Americans also want the U.S. and Europe to take a stronger position on the conflict.
BEISSINGER: The immigrant community is interested in insuring that we do something and to do something soon.
BRADY: President Obama issued a statement yesterday during his trip to Mexico.
ULANA METREKEVITCH: The United States condemns in the strongest terms the violence that's taking place.
BRADY: Back at the Ukrainian community center, rally organizer Ulana Metrekevitch(ph) read the entire statement over a loud speaker. People at this rally say they're calling their members of Congress asking for things like sanctions. The president of the Ukrainian center, Sophia Korupetski(ph), says her community is hopeful for change in Ukraine, but for now, all they can do is keep up with the news and hold rallies.
SOPHIA KORUPETSKI: I think people just feel they need to do something. And it's not gonna accomplish anything. It's just that we just want to show solidarity. We just want, you know, people driving by to just see what's going on.
BRADY: And she hopes some of those people in cars will get curious and learn more about what's happening now in Ukraine. Jeff Brady, NPR News, Philadelphia. Transcript provided by NPR, Copyright NPR.


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DES MOINES, Iowa Donating blood is safe, simple and only takes about an hour of your time. LifeServe Blood Center needs new, first time blood donors, as well as established blood donors, to help expand and sustain the donor base. Blood donors are needed every day to provide lifesaving blood products to hospital patients in our community. LifeServe Blood Center invites you to make a difference in your community and become a blood donor.
Be a contributor in your community and give your lifesaving gift at any of the following blood drive(s)!
Wellsburg Blood Drive, Friday, April 12, 2013 from 2:30 PM - 5:30 PM at Wellsburg Community Center, 511 North Adams.
Sign up to save a life today! Schedule a blood donation appointment online at www.lifeservebloodcenter.org or call 800.287.4903.


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To read all comments associated with this story, please click here.
There are a load of applications that I have to use due to work requirements. I can't really do without applications like Microsoft Office, simply because I need the compatibility they offer, not necessarily because I prefer them to the alternatives.
There are quite a few apps that I really enjoy using and really miss then they aren't available, often because I have them configured to meet my needs. EAC and Fubar2000 are two examples.
EAC is by far the best audio CD ripper I've used, essential when trying to archive a large collection to lossless files. It's supported with various 3rd party plugins and utilities to make that process quick and painless.
Foobar2000 doesn't look too impressive at first glance, but is a truly brilliant media player. There are none of the annoyances in other players (ridiculous eye-candy skins for example) and loads of useful features. For example the file tagging and conversion tools work great, making it easy to manage your collection with that one tool. The whole thing can be tweaked and expanded in countless ways, making it one of the most flexible apps I've ever used.
If I had to choose just one application I wouldn't want to live without it'd definitely be Opera. Like quite a few other people here I do a lot of web browsing, both for entertainment and research purposes. Opera is in a totally different league to any other browser I've used.
It's amazingly fast and stable even when a large number of pages are open, best of all the user interface allows that number of pages to be managed effectively. There are just so many great little touches and useful features in Opera that it's like going into the past when using an alternative. Using any other browser feels like working with one hand tied behind my back, so limited, slow and frustrating.


text:
Audio here.
ADDED: The bracketed "heyday" above replaces the Daily Caller's "hay day." Did you think someone's "heyday" had something to do with hay? Were you picturing something like this:
No! You are wrong:
late 16c., alteration of heyda (1520s), exclamation of playfulness or surprise, something like Mod.Eng. hurrah, apparently an extended form of M.E. interjection hey or hei (see hey). Modern sense of "stage of greatest vigor" first recorded 1751, which altered the spelling on model of day, with which this word apparently has no etymological connection.


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A Decatur Utilities crew left Alabama to help with storm recovery in New Jersey, but did not make the trip because of what they are now calling confusion over union paperwork.
The six-man crew staged in Virginia, but Decatur Utilities said they were never able to get in touch with officials or utility companies in New York or New Jersey. While the crew stood down in Virginia, Decatur Utilities officials claimed they received a union contract they thought they would have to agree to before helping storm victims.
General Manager Ray Hardin appeared on Fox Business Channel Friday morning saying, "We were presented documents from IBEW that required our folks to affiliate with the unions and that was something we could not agree to."
Decatur Utilities later released a statement expanding on that, "Upon arriving at a staging area in Virginia, crews were held in place pending clarification of documents received from IBEW that implied a requirement of our employees to agree to union affiliation while working in the New York and New Jersey areas. It was and remains our understanding that agreeing to those requirements was a condition of being allowed to work in those areas."
Late Friday at a press conference, Hardin said the documents actually came from Electric Cities of Alabama, a coalition of the state's municipally owned utilities.
Bottom line, it appears now that Decatur Utilities wrongly assumed they would have to agree to the union contract before traveling to New Jersey to help with recovery efforts. The IBEW said in times of crisis, help is welcomed from union and non-union utility workers.
Hardin said as they waited for confirmation on the documents, crews received word that Seaside Heights had received the assistance they needed from other sources.
"At this stage, it is not clear who is alleged to have turned the crew away and the company that employs the affected workers has denied the claim," said IBEW President Ed Hill in a statement. "IBEW local leaders in New Jersey have reiterated what has been the long standing record of our union – in times of crisis all help is welcome and we pull together with everyone to meet the needs of the public. We have communicated this to the office of New Jersey Governor Christie as well."
The crew from Decatur Utilities attempted to look for work in other areas, but Hardin said based on the uncertainty of other union requirements, they made the decision to return home after being stalled in Virginia most of the day Thursday.
Other utility crews from Alabama are still helping with storm cleanup. Huntsville Utilities said they were not turned away and are in Long Island, New York. Joe Wheeler EMC said they did not respond to New Jersey, but did travel to Maryland and headed home once they were finished.
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Starry dress
Added Jan 1, 2013
Notodden, Norway
I was bored one night in the summer vacation so I wanted to make a dress. I bought one of the Burda magazines and I just fell in love with this dress design! The pattern was easy to understand, but I had to fit it in the process. I am usually a size 36, but my bust was a bit smaller than the pattern. I think I will make this dress one more time:D (I'm new here so be kind! :3 )
Material Notes
*Printed cotton ( bought at Stoff & Stil)
*Cotton lining
*Blue piping
*a button for the halterneck
3 Comments Sign in to add a post
Jan 16, 2013, 09.22 AMby anamonde
Nice fit! like a real woman
Jan 3, 2013, 01.33 PMby asperge11
I love it and it looks amazing on you :) I fell in love with that pattern when I saw it too. I made one last summer and I'd like to make it again if I get the time.
Jan 1, 2013, 07.53 PMby Mary Athey
Wow!! I love.this. I think using the blue piping.has really made this.dress stand out. Lovely spotty fabric. And.welcome.to burdastyle. and happy.new year x
1 Reply
Jan 1, 2013, 08.01 PMby Tonje Petterson
Thank you! And happy new year :D
Add to Favorites 2 people added it


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The most brilliant, the most exquisite, (crushed) the blondest!
As they did so the blondest blonde caught sight of Grace and recognized her.
Small as it was, the cubicle was decidedly pretty, and blue enough to satisfy the blondest of mistresses.
late 15c., from Old French blont "fair, blond" (12c.), from Medieval Latin blundus "yellow," perhaps from Frankish *blund. If it is a Germanic word, it is possibly related to Old English blonden-feax "gray-haired," from blondan, blandan "to mix" (see blend (v.)). According to Littré, the original sense of the French word was "a colour midway between golden and light chestnut," which might account for the notion of "mixed."
Old English beblonden meant "dyed," so it is also possible that the root meaning of blonde, if it is Germanic, may be "dyed," as ancient Teutonic warriors were noted for dying their hair. Du Cange, however, writes that blundus was a vulgar pronunciation of Latin flavus "yellow." Another guess (discounted by German etymologists), is that it represents a Vulgar Latin *albundus, from alba "white."
The word was reintroduced into English 17c. from French, and was until recently still felt as French, hence blonde (with French feminine ending) for females. Italian biondo, Spanish blondo, Old Provençal blon all are of Germanic origin.
Fair hair was much esteemed by both the Greeks and Romans, and so they not only dyed and gold-dusted theirs ..., but also went so far as to gild the hair of their statues, as notably those of Venus de Medici and Apollo. In the time of Ovid (A.U.C. 711) much fair hair was imported from Germany, by the Romans, as it was considered quite the fashionable color. Those Roman ladies who did not choose to wear wigs of this hue, were accustomed to powder theirs freely with gold dust, so as to give it the fashionable yellow tint. [C. Henry Leonard, "The Hair," 1879]
c.1755 of a type of lace, 1822 of persons; from blond (adj.).


text: Hi again, I am still working on an issue for my sister in Italy. She is moving back to the USA and of course, wants to bring her chco. Lab, Ben, with her. I have all the info from US customs and global migration.....and now am looking into a "Pet Escort Service". If this does indeed exist, I believe they are an organization that handle transporting dogs (& other animals???) into foreign countries......and if they really exist, this may be an option for my sister, but i cannot find any info on them to date. Does anyone out there have any knowledge on this organization, and if so, would you be kind enough to pass on the info. My private email is [email protected], or post to this thread. ANY help would be greatly appreciated! Thanks in advance for your time! Jenna


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Browsing Ye Old College Inn memorabilia, 1915 - ca.1960 (MS 526) by Title
Now showing items 4-10 of 10
Rice Institute Basketball Team 1953-54 (1953)Photograph of the 1953-54 Rice Institute basketball team who won the Southwestern Conference title in 1954.
Rice Institute Football Team 1954 (1954)Rice Institute football team photograph taken in 1954, the year Rice won the Cotton Bowl beating Alabama, 28-6.
Some Pages From the Recipe Collection of Ernest Coker (1960)Recipes of Ernest Coker, host and proprietor of Ye Old College Inn, Houston.
Water Pool at Ye Old College Inn, 1921 (1921)Photograph taken in 1921 of an unidentified woman leaning across the edge of a small water pool or fountain behind Ye Old College Inn.
Ye Old College Inn Dining Room 1920 (1920)Photograph taken in 1920 of students dining in Ye Old College Inn dining room.
Ye Old College Inn Dining Room 1940 (1940)Photograph of the dining room of Ye Old College Inn taken in 1940, showing how much it expanded since the 1920's.
Ye Olde College Inn scrapbook, 1920-ca.1960 (1920)Bound scrapbook with wooden covers, 12" x 17", containing photographs and news articles of special events, Rice Institute athletes and coaches.


text: Meta Rate My Team PokéBase - Pokémon Q&A Login Register All Activity Questions Unanswered Tags Users Ask a Question Rules Chat What is the Story behind Mew and Mewtwo ? 2 votes 39,887 views I've seen some interesting videos about Mew and Mewtwo. They say that Mewtwo escaped from a scientific lab and went somewere unkown. In FireRed and LeafGreen,in Cinnabar Island there is the Pokemon Mansion.Where you can find documents that have information on Mew and Mewtwo.They say the followig sentenses: July 5 Guyana,South America,a new Pokemon has been discovered deep in the jungle. and this : July 10 We christened the newly discovered Pokemon,Mew also this one : Feb,6 Mew Gave Birth,we named the newborn Mewtwo what in the world is happening here ? Also in the Pokemon Mansion you can see Mewtwo statues with secret switces in them. in Faraway Island,in the Hoenn region,which can only be accesed from a certain event,is the place where you can catch Mew,There is a Signboard with the following Sentenses : ...Ber,6th day.If any human...sets foot here...again...et...it be a kindhearted pers... ith...that hope,I depar... what is that all about ? Who wrote this and why ? Why are the documents inside Pokemon Mansion ? and what role does the Mansion play with this story ? Has Mr.Fuji anything to do whith this ? Has Team Rocket anything to do with Mew and Mewtwo ? EDIT : The documents also say this : Sept.1 Mewtwo is far to powerful.We have failed to curb its vicious tendencies... How do these events connect with each other ? Video skip to 1.27 I've searced everywere and I can't find the answer. Please read my question carefully before you answer. mew mewtwo mystery asked Apr 7, 2013 by MK 42 edited Apr 7, 2013 by MK 42 Mewtwo was a scientific experiment gone wrong from what I remember from the first Pokemon film. It was made with dna of Mew and escaped to this island thingy. Though I never played fire red and leaf green so I don't really know the in game story and whether its linked to the anime in any way commented Apr 7, 2013 by Blobyolo ok,i'll wait for someone who has commented Apr 7, 2013 by MK 42 Blob, come on Chat please :] commented Apr 7, 2013 by Flare all these questions are freaking me out. its so mysterious and creepy. commented Apr 7, 2013 by Finchwidget blobyolo mew went to the island, mewtwo escaped to cerulean cave. commented Aug 8, 2017 by Ilola Please log in or register to add a comment. Please log in or register to answer this question. 4 Answers 10 votes Best answer So I remember watching the film. Mew is discovered in South America by a Team of Scientist lead by Mr.Fuji (Not Team Rocket). They report their findings a christen the Pokemon 'Mew'. They also find a hair sample belonging to Mew so take it back for examining. Team Rocket steal the DNA sample and use it to make a stronger clone of Mewtwo in the hope to create the most powerful Pokemon ever. Unfortunately for them Mewtwo is far too powerful and it therefore destroys the lab. Giovanni (Leader of team rocket) tricks Mewtwo into training with him to harness his power. Giovanni builds Mewtwo a suit which allows Mewtwo to harness his power. But then Mewtwo realises that he is being used as a weapon so destroys the suit and flies of into the Pokeworld. Information on the Faraway Island sign: >On the island is a sign with old, fading writing dated the 6th day of a month; in the English localization, this month ends in "ber", but in the Japanese version, it is only known that it is a month name. Also, exclusively in the Japanese version is a remnant of a signature at the end of the message, ジ ji; this is commonly assumed to be Fuji. Whether it refers to Mr. Fuji or Dr. Fuji, both of whom may actually be the same character, is unknown. This island could be the Guyanese jungle referenced in the Pokémon Mansion journals. ~ Bulbapedia answered Apr 7, 2013 by Sciz selected May 6, 2013 by Shining Yin Τhanks guys ! I really appreciate your help ! +1 to all :D commented Apr 7, 2013 by MK 42 no problemo. and thanks for the extra point. thanks a LOT. +1 to you, too commented Apr 7, 2013 by Finchwidget Please log in or register to add a comment. 1 vote i will answer your questions in order: 1.They say mewtwo went somwhere unknown? he most likely went to cerulean cave. 2.those sentences you listed are probably part of a scientists journal, going day by day, watching the process of creating mewtwo 3. thats probably some coincedince, but you never know... 4. they were probably saying stuff about, well, the player!if any human sets foot here again... it be a kind hearted person... with that hope...you get it? and I dont know who wrote it. 5.what do you mean?! mr. fuji's a nice person who lives in lavender town. if you mean DR. FUJI, thats another story... dr. fuji was the one who came up with the cloning idea and was hired by giovanni, and ran a team of scientists. after mewtwo was created, he blew up the lab and killed every one, including fuji 6.yes. after all, whos the one who hired dr. fuji to do the cloning idea? GIOVANNI! and whos the head of team rocket? GIOVANNI! I rest my case. 7. I dont know what role the mansion plays. maybe the journal was there for safekeeping, maybe the owner found it in kanto. anything's possible and how these events connect? one way or another, they all mention mew and/or mewtwo answered Apr 7, 2013 by Finchwidget edited Apr 7, 2013 by Finchwidget Please log in or register to add a comment. 1 vote ok Teod...you have almost answered you q...As you have said mewtwo was in a lab. Some scietists collected ancient DNA from Mew...then they tried to creat a clone of Mew....they created a different Pokemon that it was too strong to be kept in a laboratory. Jiovanni,the team Rocket's boss discovered the existance of Mewtwo and captured it ,to use for a bad cause of course. As for the games FireRed and L eafGreen ,GameFreak just wanted to use the legend of the 2 Pokemon and they created the cinabar's mansion for the games. FARAWAY island is the place that that mew lives.....its just a part of the game,u know rayquaza lives in sky pillar ,kyuogre in Marine cave.etc. The anime and movies have nothing to do with gemes .....they are 2 difffernt categories. P.S;it would really help if you could watch the 1st Pokemon movie. source:experience;D answered Apr 7, 2013 by DarkStar Please log in or register to add a comment. 0 votes here ill put it simply mew dna found 2.mew2 escapes mew2 defeats gary mew2 kidnaps nurse joy 5.ash goes to battle him clone fight ash dies ash comes back to life mew2 and clones go th johto 10.giovanni attacks mew2 ash saves mew2 again game mew2 born out of mew ( FIGURATIVLY OR LITERALLY) manga Red sees mew team rocket attacks mew green robs red red sees mew2 there answered Apr 10, 2013 by TeamRocketBossJ reshown Oct 8, 2013 by TeamRocketBossJ Please log in or register to add a comment. Related questions What is the password for Mew and Mewtwo dolls of the month of May in the Pokemon Dream World? Are Mew and Mewtwo based upon anything? Are Mew and Mewtwo the most powerful Pokemon? Why isnt their corresponding pokemon for mew and mewtwo??? How do you breed mew and mewtwo? Does anyone now how to get mew and mewtwo in Firered/LeafGreen? What does mew say in the first Pokemon movie, mew vs. Mewtwo? Why is Mewtwo the 150th poke'mon When mew came first? Useful Meta Posts How to change your username How to add a user avatar Why was my post hidden? The points system Question etiquette Flags vs Downvotes Anime FAQ


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The Fighting Kind (Chapman Univ.) and Northeast Front (Univ. of Texas) : Davin, estranged from his family for years, returns home in desperate need of financial help. He learns that his sister may be the sibling who's truly in need. Written, directed by Nils Taylor. Based on a true story, Esperanza's love for her children knows no bounds, but her limits are pushed when she is forced to save her son from Mexico's dangerous cartels. Written and directed by Angela Camarena Torres.
Visit the Website: http://www.kqed.org/fss
Episode #105 / Length: 28 minutes


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Jupiter is now lower in the west at dusk. Face west at dusk and look for the brightest thing there (unless the Moon is also there), as Jupiter outshines all other stars we ever see at night. It appears slightly lower in the sky each night, though, and is gone by the end of the month. As Jupiter leaves the evening sky, Mercury and Venus emerge to join it on Memorial Day weekend. The three planets form a tight triangle May 25-26; Venus and Jupiter are just one degree apart on May 27.
Saturn is now an evening object, shining in the southeast at dusk. Although not as bright as Jupiter, it does outshine the stars around it, so you can't miss it.
Mars is still out of sight on the far side of the Sun this month.
A swath of brilliant winter stars sets in the west at dusk. Orion, the Hunter, is still visible in the west as May begins. His two dogs, represented by Sirius and Procyon, are to his left. To Orion's right is Taurus, the Bull, with Aldebaran as its eye. Gemini, the Twins, are above Orion. The Big Dipper is above the North Star, with its handle pointing to the right. From that handle, you can 'arc to Arcturus' and then 'speed on to Spica'; those stars are in the east and southeast at dusk. Leo, the Lion, passes almost overhead in late evening.
As Orion and Taurus set, look for Antares, brightest star of Scorpius, the Scorpion, to rise in the southeast. At the same time, Vega, brightest star of the Summer Triangle, appears low in the northeast. These stars remind us that summer is on the way.
Moon Phases in May 2013:
Last Quarter May 2, 6:16 am; May 31, 1:59 pm
New May 9, 7:31 pm
1st Quarter May 17, 11:35 pm
Full May 24, 11:26 pm
The New Moon of May 9 passes in front of the Sun, causing an eclipse. However, the Moon is too far from the Sun to block it completely; the result is an annular eclipse, in which a ring of the Sun's disk surrounds the Moon. Also, the event is visible only along a path that begins in Australia and extends across the Pacific.
On most clear Saturday nights at the George Observatory, you can hear me do live star tours on the observation deck with a green laser pointer. If you're there, listen for my announcement. I generally do one such tour on short May nights.
To enjoy the stars in any weather from the comfort of the HMNS Planetarium, click here for a full schedule.


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Creating a lean palm version would be a million miles away.
Hardly. They could just do this on their source code to make it smaller
s/.*linux is the devil.*\n//gi
That should drop about 50% of their code size
Apple also has complete control over the hardware specs their software is supposed to run on, which must considerably narrow the complexity of their hardware interfaces. That's why Apple makes whole computers (or devices) and doesn't separate their hardware from their software.The other major difference is Apple doesn't have this horrid codebase that Microsoft does.
I think this argument is incorrect. MS doesn't spend a lot of time making their OS work on every hardware combination, rather because of their monopoly position they can just release whatever they have knowing that hardware makers will write their own drivers and do whatever else is needed to make it work with Windows, since otherwise they aren't going to make any sales. Heck, Vista has removed hardware support for some motherboards and even things like TCP/IP over Firewire. MS isn't the one doing the work to make Vista work on all hardware and hardware makers will even change their hardware designs in order to make them work with Windows.
Apple, on the other hand, targets a subset of hardware themselves and works with the hardware vendors to make it work, and deals with extremely large problems getting drivers for and third-party add on hardware like video card upgrades, web cams, external drives, etc. A lot more of that work does require Apple to intervene and make things really easy for hardware makers, because they usually can afford to walk away from providing mac support if it is problematic.
Apple makes whole computers and won't license their OS to OEMs (who do most of the work making hardware run with an OS) because the market is destroyed at this point. They even tried going that route back in the 90s and had to cancel it not because of hardware support problems, but because they were damaging their brand because a lot of the OEMs were using really cheap and crappy hardware that often failed and at the same time had the same bullet points as Apple's hardware but at a lower price. Basically, when the desktop OS market is monopolized, try to compete therr is a doomed venture and Apple and several other vendors discovered.
Apple ties their hardware and OS because it allows them to sell systems based upon the features of the OS, while at the same time competing in the computer system market which is still relatively healthy (against Dell, Sony, etc.) instead of trying to compete against MS in the desktop OS market, which has been completely undermined by MS's monopoly.
For how many years have slashdot 'experts' been predicting the 'downfall' of windows? For 23 years they have not just controlled, the word is 'dominated' the desktop environment. For the majority of computer users, the words 'Windows' and 'Computer' are borderline synonymous.
And you're proof? Because some users believe that 'Vista sucks' blah blah blah. How many people started ringing the bells for Microsoft after Windows ME? We saw how that worked out...
Windows will die like a big ole dinosaur, and its death throes are gonna mess up its local ecosystem real bad.
Stay well clear of that tail. There's no mind controlling it any more.
And... well... there's no pleasant way to say this, but it needs to be said. So WARNING: NEXT PARAGRAPH MAY EVOKE UGLY GRAPHIC IMAGERY!
When a dinosaur dies like Windows is dying, it not only thrashes around a lot, but all its sphincter muscles relax and contents of its bowels and bladder spew forth, driven by the pressures of the terminal seizure. You want to be on high ground and up wind when that happens.
Unfortunately for MS, virtually the entire world's population now has Windows experience. It was not a great experience.
Some are cretins, and could not interface with a 4x2, but enjoy blaming windows
Some are experienced IT people who have seen Linux/Unix and know how it could be.
Most are now in a position to ask the professionals "Is this as good as it gets?" and being told - no, there IS another way.
Some are migrating to Vista, and realising that if it can get worse, sure as hell it could get better somehow. They know who to ask for advice, and its not the guy in PC world.
Because Apple is even more expensive and just as proprietary as Windows, won't let me build my own system, and is poorly supported by software developers. If Apple dominated the market, there is every reason to believe they would be just as heavy-handed as MS, if not much worse.
Because doing anything in Linux ends up with me banging my head against my computer screen. Even Ubuntu, the most user-friendly distro so far, is an endless series of frustrations. "Why can't I just download a piece of software and double-click on it to install?!?!" "What is the difference between KDE and Gnome and why should it matter?!?!" "Why do I have to go to the command line interface to do even basic stuff?" Hell, until the latest release, Ubuntu wouldn't even let me attach a projector without a complicated edit to the Xorg config file. ARGHHHHH!!!
Windows may die one day, but it's going to take a *lot* more work before anyone else is going to slay that dragon.
As a Linux user I have the opposite frustrations when I come to use Windows. "Why do I have to search the web to find a piece of software to download? Why can't I just go to 'Add/Remove Programs', type in the name (or a keyword) and click install?", "Why can't I chose a different desktop environment when I log in?", "Why can't I use the command line to do even basic stuff?"
Different strokes for different folks.
I can see this happening rather quickly at home. It hasn't been hard to convince my family members to get away from Windows. While my wife is probably more computer savvy than most, she hasn't had any problems switching from Windows to Linux, and actually likes it more. It's been more difficult for others I've gotten to switch, but in general the result has been positive.
The corporate world is a completely different story, though. Many large, medium, and small companies have committed vast resources to development in
Additionally, while you can probably count on your IT staff to have a reasonably easy transition to something other than Windows, your non-tech employee base is almost certainly going to have a great deal of difficulty. Add in the fact that lots of small and mid-size businesses use "friendly" accounting software that runs solely on Windows, and I think Microsoft has a much larger buffer for error than most people think.
Will it happen? God I hope so... but I'm not optimistic it will happen even in the next 5-10 years.
When a technology service becomes ubiquitous and homogenous and - importantly - ceases being innovative, it runs the risk of becoming a candidate for conversion into a public utility. To stave this off, either ongoing innovation is required or the illusion of innovation and change is required. Microsoft has done a bit of both with Windows. But it's a thin veneer. As a result, poopulist efforts to 'socialize' this technology into a public utility are surging; hence, Ubuntu et al.
Of course Windows is going to decline.
The International Monetary Fund [telegraph.co.uk] just announced that the sub-prime crisis has tipped the USA into the worst recession since the Great Depression of the 1930s. During recessions, the first thing to get cut back on is unnecessary infrastructure replacement -- and PCs have been marketed on the basis of planned obsolescence [wikipedia.org] for around a decade now. So the PC replacement cycle will be hit, hard.
Vista is a resource hog, Ubuntu is just about coming up to mass market usability, and a lot of places are going to stop replacing their PCs annually or bi-annually in the next couple of years. Unless Windows 7 is as comparatively lightweight as XP, it's going to crash in the "upgrade your OS" market -- only new PCs will ship with it. So Microsoft will have two poor sellers in a row -- which is enough, in the mind of the fickle public, to establish a trend, and with Apple chowing down on 25% of the high-end laptop market already, they're in danger of being squeezed between a high-end competitor and a low-end one.
Windows is so big, with such a huge established base, that its decline will resemble that of the old IBM mainframe environment -- which is still doing fine, decades after the death of the mainframe was predicted. This ain't going to happen overnight.
However, if you read on, you realize this is just a bit of journalistic bluster since the WEO is predicting the US economy to grow this year (just grow slowly). Growing slowly isn't a recession at all - I b
An elephant is a mouse with an operating system.


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30 Summer Salads
We're in the middle of summer right now and summer is definitely the salad season! Salads are amazingly versatile in that they can contain so many different things from a basic lettuce, cucumber and tomato salad to chicken salads, to potato salads, etc., they can be served either hot or cold and they can be everything from a side dish to a main course. After a long hot summer day I somethings don't feel like cooking, or eating a hot or heavy meal and a light, cool and crisp salad is the perfect solution! Summer is a great time for salads since there are so many fresh vegetables and fruits that can be used in them and as the ones that are available change, your salads change ensuring that they are always exciting! After thinking about salads so much I figured that it was about time that I gathered a few of my favourites and shared them with you and below you have 30 of them along with a few tasty dressings!
30 Summer Salads
Spinach Salad with Bacon, Caramelized Onions, Mushrooms and Blue Cheese in a Bacon Pan Sauce Dressing Topped with a Hard Boiled Egg


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Spring 2018 Registration Now Open! Register before the end of the season and save 10%!
Slate of candidates for the 2017 Redding Youth Soccer League Board of Directors
Spring 2018 Registration Now Open! Register before the end of the season and save 10%!
Redding Youth Soccer provides a healthy activity through its recreational and small sided games programs. These programs emphasize FUN, and de-emphasize winning at all costs. Every child is guaranteed playing time and the game is taught in a fun and enjoyable atmosphere where EVERY coach is certified and licensed to be teaching this great sport.
For the player with advanced skills and greater ambition, highly competitive leagues are offered. This player might have his or her sights set on a college soccer scholarship or playing for the U. S. Olympic or National teams.
Redding Youth Soccer is a non-profit organization whose mission is to foster the physical, mental and emotional growth and development of Redding's youth through the sport of soccer at all levels of age and competition. Our job is also to make it fun, and instill in young players a lifelong passion for the sport.
RYSL has two main registration periods. January for the Spring Season and May for the Fall Season of play. We notify all members via direct mail, the website, email, newspaper and other media outlets of the upcoming registration events.
read more >>
We get asked some of the same questions on a regular basis. We've tried to cover the most frequent ones on the FAQ page. Everything from season length to what your child needs to play. Perhaps you can find your question and answer here? If not, feel free to drop us a line!
read more >>
To provide a program dedicated to develop and sustain a quality, consistent, safe and positive environment to assist youth soccer players in attaining their highest possible level of personal skills, tactics, sportsmanship and overall soccer proficiency through proper teaching and competition. The clubs philosophy is that development of players and how we play the game is a greater priority than winning.
read more >>
PO Box 991523; Redding, CA 96099-1523 | Sorry, no phone number available. | email@example.com |


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Remember Haze? You'll be forgiven if you can't recall the critically-panned PS3-exclusive shooter from 2008. It was the last game to come from Free Radical Design, before being transformed into Crytek UK.
Haze's failure ultimately hurt the prospects of a long-requested fan project: TimeSplitters 4. Free Radical had successfully launched three games in the series, but publishers couldn't help but be concerned after the game's disastrous release.
Former employee Steve Ellis told GamesTM (via Joystiq) about the company's struggle to get TS4 made. He noted that publishers "would ask what happened with Haze. We were the company that made a series of high-rated shooters and then we had released Haze, which wasn't as well received. This worried them."
Haze wasn't the only reason TS4 was denied a greenlight. The franchise was never a tremendous sales success either, and marketing the title would prove to be difficult. "The unanimous opinion among all publishers that we pitched TimeSplitters 4 to is that you can't market a game that is based around a diverse set of characters and environments – you need a clear and easily communicated marketing message, and TimeSplitters doesn't have one."


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Well, I've just used the worst word processor ever. I started using word processors back with Windows 3.11 and have been through a multitude of different products from different companies. Microsoft Office 2007 Word is absolutely garbage.
I have a new customer whose "friend" had tried to set-up their new laptop a number of months ago. It started with Windows Live OneCare that wasn't installed correctly and wouldn't do any updates. I spent nearly an hour on the phone to Microsoft help and ended up talking to the US help desk - "Where are you calling from, Bathurst, Australia, is that near Canada?" - but finally got a working Windows Live OneCare. There is still an extra computer in the circle but I can't get rid of it and neither could Microsoft!
The customer wants a basic User Guide to help with file management, Internet connection and email. I started with doing screen shots on the laptop and decided that it would be easy enough to use Microsoft Word 2007 to write up simple instructions and include the appropriate screen shots. It became really frustrating with placing graphics and setting text wrap to "top and bottom". The images would jump everywhere all over the page and previous pages. As soon as they jumped before the previous image the automatic caption numbering would get out of sequence. This meant either deleting the two images and having another go or dragging the caption box to the correct image sequence.
I finally got the document finished and decided to publish to PDF - I then found that I'd saved the document in *.docx format and opening with OpenOffice.org 2.4 on my Ubuntu 8.04 system didn't bring the images across. So I pinched my Vista laptop off my daughter, I had to get one to use Cisco SDM a couple of months ago , and went to print the document using Adobe Acrobat. It took me 15 minutes to find where the "Print" menu is located in Microsoft Office 2007 Word. I went through every "ribbon menu" and checked all of the options available in each section. I tried the little menu where "Save" and "Undo" icons reside. I eventually found the hidden menu that appears when you click on the Office Logo at the top left of the window.
It's not like I'm computer illiterate, or have only used one operating system or only used one obscure word processor but with over 15-years computer experience and over 8-years teaching IT part-time I felt basically incompetent using a word processor.
It really is the time of Linux on the desktop, and with more and more "netbook"/mini-notebooks at low prices running customised versions of Linux, it can't happen quick enough for me.


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Radisson Hotels Val Morin hotels are provided below. Search for cheap and discount Radisson Hotels hotel prices in Val Morin, QC for your family, individual or group travels. We list the best Radisson Hotels Val Morin hotels + motels so you can review the Val Morin Radisson Hotels hotel list below to find the perfect place.
Our page will also provide discounted Val Morin, QC hotels that have a comparable rating of a Radisson Hotels.


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Steven Gerrard believes the signing of Philippe Coutinho could help Liverpool take a step to the next level.
The skilful 20-year-old Brazilian has made an immediate impact at Anfield, although he is yet to play a full 90 minutes for the Reds.
After scoring once, assisting twice, and wowing the Anfield crowd with a series of deft touches and skills in his three starts so far, captain Gerrard has been quick to praise the star who is showing early potential of becoming a Liverpool hero.
"He's got moments of magical brilliance," claimed Gerrard.
"I think you'll see the best of him next season when he gets a full pre-season under his belt and gets used to the speed of the game.
"What he has shown for 55 or 60 minutes is that he's a top player and he belongs in a Liverpool shirt.
"If we can get that for 90 minutes from him next season then he's going to be a great asset for us."
Coutinho has not played regular football since his loan spell at Espanyol last season, where he netted an impressive six times in 15 games from midfield.
After scoring on his full debut for Liverpool following his £8.5 million January switch from Inter Milan, the little Brazilian has had no problems settling in at Anfield with pundits, fans, and team-mates alike admiring his instant impact.
Liverpool full-back Jose Enrique agrees with his captain:
"The numbers speak for themselves: in four games he has two assists and one goal so I hope he will continue like this scoring goal and making assists," he said.
"He is the type of a player, as a big team, you have to have.
"He can create from nothing in a one versus one. He is playing really well right now and he is the type of player we need.
"Defenders are important but you need people who can score goals and we have now."
DISCLAIMER: This article has been written by a member of the GiveMeFootball Writing Academy and does not represent the views of GiveMeFootball.com or SportsNewMedia. The views and opinions expressed are solely that of the author credited at the top of this article. GiveMeFootball.com and SportsNewMedia do not take any responsibility for the content of its contributors.
Do YOU want to write for GiveMeSport? Get started today by signing-up and submitting an article HERE: https://www.givemesport.com/writeforgms


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Transformation problem. Please help!
Posted 03 August 2009 - 09:23 PM
I've been struggling with one transformation for months and I really need some advices from you guys.
This is my case:
I have an 1.7kb insert and it has been double digested w/ EcoRV and XhoI to create a blunt end and a sticky end. I performed the same (double) digestion on the vector (pcDNA3.0-FLAG, 5.4kb).
Next, I performed the ligation (so far I'd tried 2 ligation kits: Takara DNA Ligation Kit & Fermentas Rapid DNA Ligation Kit) and then I immediately made the transformation into DH5a cells.
However, I can rarely get colonies w/o insert using Takara Ligation kit and no colonies at all using Fermentas DNA Ligation kit. I have no idea what had gone wrong since I followed the protocol accordingly.
I'll write down the steps which I'd done during the ligation and transformation. Please comment.
Ligation - Takara kit
Different ratio of insert:vector volumes (from 10:1 to 3:1) had been tried.
Same volume of ligation buffer was added and mixed
The mixture was incubated @ 16degC for 30min
10ul of ligation mixture was used for transformation
Ligation - Fermentas Kit
3:1 of Insert:vector (volume ratio) had been tried so far
4ul of 5x ligation buffer was added
1ul of T4 DNA ligase was added
Autoclaved H2O was added to fill the ligation mixture up to 20ul
The mixture was mixed and incubated @ 22degC for 5min
5ul of ligation mixture was used for transformation
Transformation (DH5a)
Each tube contained 50ul of competent cells was used.
The cells were thawed on ice for 10min after taking out from -80degC
The ligation mixture was added directly into the tube
The tube was rested on ice for 30min
The cells were heatshocked @ 42degC for 20sec
The tube was rested on ice for 2min
950ul of prewarmed LB was added to the tube thereafter
The cells were incubated @ 37degC waterbath, shaking @ 300rpm for 1h
200ul of the mixture was spreaded to LB plate containing ampicillin (twice)
Posted 03 August 2009 - 11:27 PM
Posted 04 August 2009 - 12:01 AM
Posted 04 August 2009 - 12:39 AM
I always perform blunt end ligation at 16°C over-night whatever ligase I use.
I agree - I always do a 16degC ligation overnight for blunt ends. You can also add 2ul of 50% PEG 4000 (for a 20ul ligation) to increase the chances of the blunt ends ligating (it worked for me in my magic 3 way ligation with blunt ends!).
Good luck
Posted 04 August 2009 - 12:56 AM
Posted 04 August 2009 - 01:10 AM
On the transformation: do you have a positive control for transformation? I think 10ul is too much DNA for the cells, and definitely heat shock at 42C for at least 60sec (I also always do 90sec). Also, I usually only add ~250ul after the heat shock and 2min on ice before incubation at 37C. Alternatively you could spin down after the 37C incubation to concentrate the cells before you plate.
Posted 04 August 2009 - 02:14 AM
I'll try overnight ligation and let you all know about the outcome.
lizzy & almost a doctor:
I'll try to extend the heatshock period to 60 sec; I hope I won't cause any harm to the cells
In fact, the amount of LB added prior to 1h shaking @ 37C was also one of my concern since I'll have about 600ml of them left after spread plate. I'd like to find out any good recommendation to break the pellet after spinning them down? Gently tapping on tube wall or...?
Thanks for your advices
Posted 04 August 2009 - 02:50 AM
I use no more than 2 µL for 50 µL of bacteria.
For the plating, what I do is :
I add LB too, the final volume is 1 mL.
I plate 100 µL, and I centrifuge the 900 µL left, resuspend in 100 µL and plate it too. Then I get 10 times more colonies on this second plate.
Posted 06 August 2009 - 06:25 PM
I failed my transformation, again. No colony found on plate1 while satelites were spotted on plate2 (which I think could be due to contamination).
I run a gel on the ligation mixtures which I left on last two attempts (5 min ligation L3 & overnight L4), a ligation w/o insert (control insert-) L5 and the insert + vector (control ligase-) L6 (please ignore L2). Kindly refer to the attached gel image and comment.
Thank you.
Edited by Ayukawa, 06 August 2009 - 08:12 PM.
Posted 06 August 2009 - 11:14 PM
second, could you have a control where you cut your vector and re-ligate to be sure your ligase is working ?
Posted 16 August 2009 - 05:57 PM
I know it's a little stupid question, but it has happened here right now : could you check again that you are using the right antibiotic?
second, could you have a control where you cut your vector and re-ligate to be sure your ligase is working ?
Sorry to reply late. I was told to do a in situ hybridisation during the past week.
I can confirm that right antibiotic is used since my colleague prepared the agar plates with me
I'm not pretty sure about the control you mentioned but the ligase used was newly arrived in late July and I think there shouldn't be any problem.
Posted 17 August 2009 - 05:54 AM
I know it's a little stupid question, but it has happened here right now : could you check again that you are using the right antibiotic?
second, could you have a control where you cut your vector and re-ligate to be sure your ligase is working ?
Sorry to reply late. I was told to do a in situ hybridisation during the past week.
I can confirm that right antibiotic is used since my colleague prepared the agar plates with me
I'm not pretty sure about the control you mentioned but the ligase used was newly arrived in late July and I think there shouldn't be any problem.
I'm not sure if your DNA was dissolved in TE buffer. If yes, then probably you should just dissolve the DNA in sterile distilled water. The presence of EDTA in TE buffer might interfere with your ligation.
Also, have you tried the ratio of 1 vector to 1 insert?
Good luck.
Posted 17 August 2009 - 07:25 PM
Tell us in detail how you are cutting and purifying your DNA.
Tell us what concentration of DNA you are using in your ligation, in what volume.
Tell us what controls you have done to confirm that your transformation efficiency is high.
Posted 17 August 2009 - 11:35 PM
Insert (1741bp)
50ul of insert was obtained through PCR amplification (from originally 1ul of DNA at a concentration of 1ug/ul) using PfuTurbo DNA Polymerase
The PCR product was purified using QIAquick PCR purification kit; 50ul of EB buffer was used for DNA elution
Vector (5.4kbp)
The vector used was pcDNA3.0-FLAG.
Digestion (insert)
Enzyme: XhoI(3ul) + EcoRV(3ul)
Buffer: 10x Tango (10ul, final conc = 2x as recommended by Fermentas)
DNA: 30ul
dH2O: 4ul to make up a final volume of 50ul
Digestion (vector)
Enzyme: XhoI(3ul) + EcoRV(3ul)
Buffer: 10x Tango (6ul, final conc = 2x as recommended by Fermentas)
DNA: pcDNA3.0-FLAG (5ul @ 1ug/ul app.)
dH2O: 13ul to make up a final volume of 30ul
*Both insert & vector were incubated overnight @ 37 degC waterbath
Gel excision
-Both insert and vector were cut under low power UV illuminator using DNase-free blade
DNA Extraction (Fermentas DNA Extraction Kit) (same for insert and vector)
The excised gel were weighed and required volume of binding solution(4.5x) & TBE conversion buffer(0.5x) were added accordingly (0.01g = 10ul app. as recommended by Fermentas DNA extraction kit).
-The mixture (gel+binding solutions+TBE conversion buffer) was incubated @ 55 degC for 5min (w/ mixing in every 2min)
-After the gel was completely melted, 10ul of silica suspension was added (to bind DNA), mixed and incubated @ 55degC (w/ mixing in every 2min)
-The silica was pelleted using table centrifuge, supernatant was removed thereafter.
-The pellet was completely resuspended in prepared ice cold washing solution. Again, the silica was pelleted again to remove supernatant. This was done 3 times according to Fermentas protocol.
-After the last wash, the pellet was air-dried.
-10ul of TE buffer was used to dissolve DNA. The pellet was resuspended and incubate @ 55degC for 5min. Then, the silica was pelleted and supernatant containing DNA was extracted. (This step was repeated 3 times)
With all steps described above, I obtained the digested insert and vector.
As mentioned, I tried different ins:vec ratios (5:1, 4:1, 3:1), different incubation time (5min, 30min, overnight) with 2 different ligation kits
Kit 1 - Takara DNA Ligation Kit (Takara Mighty Mix)
- Equivalent amount of Takara Mighty Mix to that of insert+vector was added and incubated @ 16 degC, 30min
Kit 2 - Fermentas Ligation Kit (T4 DNA Ligase) (newly purchased)
- Insert+vector (volume 1)
- 5x Ligation buffer (4ul, final 1x)
- T4 DNA Ligase (1ul)
- dH2O (volume 2) was added to make up a final volume of 20ul
- incubate @ 22 degC, 5min (tried 16 degC overnight using this kit)
Transformation using DH5alpha.
With Takara kit, I managed to get colonies w/o insert. I can get no colony using Fermentas' so far.
Edited by Ayukawa, 17 August 2009 - 11:38 PM.
Posted 18 August 2009 - 06:37 AM
Always ligate a positive control (cut with one enzyme only), Always ligate a negative control (double cut and w/o insert added) and a number of ratio mixtures with the vector and insert together.
Ligate the mixtures at ~16°C (normally I place the tubes at the end of the day in room temperature water in the refrigerator) overnight!
You can add an transformation enhancer like TRAEN if you want and transform 2-10ng in competent DH5alpha.
Control the amount antibiotics in the agar before plating! Grow the transformants overnight at 35°C and the next day small colonies will appear (you can set the temperature to 37°C until the colonies are big enough)
Good luck! Greetz memo


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IGN: Espionage, giant robots, and cigarettes. The Metal Gear series has shaped, if not guided, stealth-style video games for the last 25 years. From the breathtaking victories won off the coast of Alaska to the crushing melancholy in a field of flowers, we've followed Snake through it all.
At least IGN gives props to the Medal Gear franchise, G4TV is such a hack network, they ran their best 100 games of all time list over 5 shows, filled with crap games and mostly zelda and mario games (shows you the people making the list are probably 35-40 years old) and not a single Medal Gear game was on the list, no matter how revolutionary the franchise was to stealth games, and no matter how man imitators their were that couldn't live up.
Good job IGN!
So many things to love about this franchise...one of them is,
When the sun is setting moving around the Big Shell, the "Twilight Sniping" soundtrack playing and the sad aftermath going to the Shell 1 core ...I cried


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The Sanibel-Captiva Lions Club will again provide free screenings for glaucoma, diabetes, blood pressure and hearing October thru April. These screenings assess the most insidious problems - those for which people may be totally unaware and may create irreversible damage.
All screens will take place at the Sanibel Recreation Center from 9:30 a.m. to 12:30 p.m. and are open to members and non-members alike.
The days and dates are:
2012 - Mon. Oct 22; Fri. Nov 16; Sat. Dec 15.
2013 - Fri. Jan 11; Mon. Jan 28; Wed. Feb 6,; Tues. Feb 26; Mon. March 11; Thurs. March 21; Mon. April 15.
For questions please call Tom Rothman at 395-3248.