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Articles by Shimako Kawauchi
Other Publications (13)
- Microscopy Research and Technique
- The EMBO Journal
- Brain Research. Molecular Brain Research
- Nature Genetics
- Developmental Neuroscience
- Experimental Cell Research
- Development (Cambridge, England)
- Biochemical and Biophysical Research Communications
- Development (Cambridge, England)
- Annals of the New York Academy of Sciences
- PLoS Genetics
- The Journal of Biological Chemistry
- Biochimica Et Biophysica Acta
Articles by Shimako Kawauchi in JoVE
Пластиковые вложения и секционирования Xenopus laevis Эмбрионы Souichi Ogata1, Shimako Kawauchi1, Anne Calof2, Ken W.Y. Cho1 1Department of Developmental and Cell Biology, University of California, Irvine (UCI), 2University of California, Irvine (UCI) Пластиковые разделы сохранить истинный морфологии ткани в тонких срезов тканей, которые могут быть immunostained с люминесцентными вторичными антителами, что делает этот метод более полезным, чем парафин или замороженных срезов для многих типов тканей. Метод окрашивания, пластик вложение и секционирования показано в этом видео.
Other articles by Shimako Kawauchi on PubMed
Progenitor Cells of the Olfactory Receptor Neuron Lineage Microscopy Research and Technique. Aug, 2002 | Pubmed ID: 12203696 The olfactory epithelium of the mouse has many properties that make it an ideal system for studying the molecular regulation of neurogenesis. We have used a combination of in vitro and in vivo approaches to identify three distinct stages of neuronal progenitors in the olfactory receptor neuron lineage. The neuronal stem cell, which is ultimately responsible for continual neuron renewal in this system, gives rise to a transit amplifying progenitor identified by its expression of a transcription factor, MASH1. The MASH1-expressing progenitor gives rise to a second transit amplifying progenitor, the Immediate Neuronal Precursor, which is distinct from the stem cell and MASH1-expressing progenitor, and gives rise quantitatively to olfactory receptor neurons. Regulation of progenitor cell proliferation and differentiation occurs at each of these three cell stages, and growth factors of the fibroblast growth factor (FGF) and bone morphogenetic protein (BMP) families appear to play particularly important roles in these processes. Analyses of the actions of FGFs and BMPs reveal that negative signaling plays at least as important a role as positive signaling in the regulation of olfactory neurogenesis.
HLF/HIF-2alpha is a Key Factor in Retinopathy of Prematurity in Association with Erythropoietin The EMBO Journal. Mar, 2003 | Pubmed ID: 12606578 An HLF (HIF-1alpha-like factor)/HIF-2alpha-knockout mouse is embryonic lethal, preventing investigation of HLF function in adult mice. To investigate the role of HLF in adult pathological angiogenesis, we generated HLF-knockdown (HLF(kd/kd)) mice by inserting a neomycin gene sandwiched between two loxP sequences into exon 1 of the HLF gene. HLF(kd/kd) mice expressing 80-20% reduction, depending on the tissue, in wild-type HLF mRNA were fertile and apparently normal. Hyperoxia-normoxia treatment, used as a murine model of retinopathy of prematurity (ROP), induced neovascularization in wild-type mice, but not in HLF(kd/kd) mice, whereas prolonged normoxia following hyperoxic treatment caused degeneration of retinal neural layers in HLF(kd/kd) mice due to poor vascularization. Cre-mediated removal of the inserted gene recovered normal HLF expression and retinal neovascularization in HLF(kd/kd) mice. Expression levels of various angiogenic factors revealed that only erythropoietin (Epo) gene expression was significantly affected, in parallel with HLF expression. Together with the results from intraperitoneal injection of Epo into HLF(kd/kd) mouse, this suggests that Epo is one of the target genes of HLF responsible for experimental ROP.
Effect of Acoustic Stress on Glucocorticoid Receptor MRNA in the Cochlea of the Guinea Pig Brain Research. Molecular Brain Research. Dec, 2003 | Pubmed ID: 14667579 As a first step toward elucidating the mechanism underlying the therapeutic effect of glucocorticoids on acute noise-induced hearing loss, we used semiquantitative reverse transcription polymerase chain reaction to study the level of glucocorticoid receptor (GR) mRNA in the cochlea of the guinea pig after acoustic overstimulation. The cochleas were dissected and divided into three portions (lateral portion, medial portion and modiolus). In the lateral portion, the glucocorticoid receptor mRNA level was significantly decreased 2 h after exposure to a 120 dB SPL sound and both 2 and 6 h after exposure to a 130 dB SPL sound. To determine where in the cochlea the changes in glucocorticoid receptor mRNA levels occur, in situ hybridization histochemistry was performed with digoxigenin-labeled sense and antisense RNA probes complementary to guinea pig glucocorticoid receptor mRNA. Glucocorticoid receptor mRNA was demonstrated in the spiral ligament, stria vascularis, spiral limbus and spiral ganglion. Moreover, the glucocorticoid receptor mRNA level was decreased in the spiral ligament, especially in the spiral prominence of the basal turn, 2 h after exposure to the 130 dB SPL sound. These results could imply that the therapeutic effect of glucocorticoids in the cochlea might be mediated in the spiral ligament, particularly in the spiral prominence.
Cornelia De Lange Syndrome is Caused by Mutations in NIPBL, the Human Homolog of Drosophila Melanogaster Nipped-B Nature Genetics. Jun, 2004 | Pubmed ID: 15146186 Cornelia de Lange syndrome (CdLS; OMIM 122470) is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation; abnormalities of the upper limbs; gastroesophageal dysfunction; cardiac, ophthalmologic and genitourinary anomalies; hirsutism; and characteristic facial features. Genital anomalies, pyloric stenosis, congenital diaphragmatic hernias, cardiac septal defects, hearing loss and autistic and self-injurious tendencies also frequently occur. Prevalence is estimated to be as high as 1 in 10,000 (ref. 4). We carried out genome-wide linkage exclusion analysis in 12 families with CdLS and identified four candidate regions, of which chromosome 5p13.1 gave the highest multipoint lod score of 2.7. This information, together with the previous identification of a child with CdLS with a de novo t(5;13)(p13.1;q12.1) translocation, allowed delineation of a 1.1-Mb critical region on chromosome 5 for the gene mutated in CdLS. We identified mutations in one gene in this region, which we named NIPBL, in four sporadic and two familial cases of CdLS. We characterized the genomic structure of NIPBL and found that it is widely expressed in fetal and adult tissues. The fly homolog of NIPBL, Nipped-B, facilitates enhancer-promoter communication and regulates Notch signaling and other developmental pathways in Drosophila melanogaster.
Molecular Signals Regulating Proliferation of Stem and Progenitor Cells in Mouse Olfactory Epithelium Developmental Neuroscience. Mar-Aug, 2004 | Pubmed ID: 15711058 To understand how signaling molecules regulate the generation of neurons from proliferating stem cells and neuronal progenitors in the developing and regenerating nervous system, we have studied neurogenesis in a model neurogenic epithelium, the olfactory epithelium (OE) of the mouse. Our studies have employed a candidate approach to test signaling molecules of potential importance in regulating neurogenesis and have utilized methods that include tissue culture, in situ hybridization and mouse genetics. Using these approaches, we have identified three distinct stages of stem and transit amplifying progenitor cells in the differentiation pathway of olfactory receptor neurons (ORNs) and have identified mechanisms by which the development of each of these progenitor cell types is regulated by signals produced both within the OE itself and by its underlying stroma. Our results indicate that regulation of olfactory neurogenesis is critically dependent on multiple signaling molecules from two different polypeptide growth factor superfamilies, the fibroblast growth factors and the transforming growth factor beta (TGF-beta) group. In addition, they indicate that these signaling molecules interact in at least two important ways: first, opposing signals converge on cells at specific developmental stages in the ORN pathway to regulate proliferation and differentiation; and second, these signaling molecules--particularly the TGF-betas and their antagonists--play key roles in feedback loops that regulate the size of progenitor cell pools and thereby neuron number, during development and regeneration.
Identification and Molecular Regulation of Neural Stem Cells in the Olfactory Epithelium Experimental Cell Research. Jun, 2005 | Pubmed ID: 15925585 The sensory neurons that subserve olfaction, olfactory receptor neurons (ORNs), are regenerated throughout life, making the neuroepithelium in which they reside [the olfactory epithelium (OE)] an excellent model for studying how intrinsic and extrinsic factors regulate stem cell dynamics and neurogenesis during development and regeneration. Numerous studies indicate that transcription factors and signaling molecules together regulate generation of ORNs from stem and progenitor cells during development, and work on regenerative neurogenesis indicates that these same factors may operate at postnatal ages as well. This review describes our current knowledge of the identity of the OE neural stem cell; the different cell types that are thought to be the progeny (directly or indirectly) of this stem cell; and the factors that influence cell differentiation in the OE neuronal lineage. We review data suggesting that (1) the ORN lineage contains three distinct proliferating cell types--a stem cell and two populations of transit amplifying cells; (2) in established OE, these three cell types are present within the basal cell compartment of the epithelium; and (3) the stem cell that gives rise ultimately to ORNs may also generate two glial cell types of the primary olfactory pathway: sustentacular cells (SUS), which lie within OE proper; and olfactory ensheathing cells (OEC), which envelope the olfactory nerve. In addition, we describe factors that are both made by and found within the microenvironment of OE stem and progenitor cells, and which exert crucial growth regulatory effects on these cells. Thus, as with other regenerating tissues, the basis of regeneration in the OE appears be a population of stem cells, which resides within a microenvironment (niche) consisting of factors crucial for maintenance of its capacity for proliferation and differentiation.
Fgf8 Expression Defines a Morphogenetic Center Required for Olfactory Neurogenesis and Nasal Cavity Development in the Mouse Development (Cambridge, England). Dec, 2005 | Pubmed ID: 16267092 In vertebrate olfactory epithelium (OE), neurogenesis proceeds continuously, suggesting that endogenous signals support survival and proliferation of stem and progenitor cells. We used a genetic approach to test the hypothesis that Fgf8 plays such a role in developing OE. In young embryos, Fgf8 RNA is expressed in the rim of the invaginating nasal pit (NP), in a small domain of cells that overlaps partially with that of putative OE neural stem cells later in gestation. In mutant mice in which the Fgf8 gene is inactivated in anterior neural structures, FGF-mediated signaling is strongly downregulated in both OE proper and underlying mesenchyme by day 10 of gestation. Mutants survive gestation but die at birth, lacking OE, vomeronasal organ (VNO), nasal cavity, forebrain, lower jaw, eyelids and pinnae. Analysis of mutants indicates that although initial NP formation is grossly normal, cells in the Fgf8-expressing domain undergo high levels of apoptosis, resulting in cessation of nasal cavity invagination and loss of virtually all OE neuronal cell types. These findings demonstrate that Fgf8 is crucial for proper development of the OE, nasal cavity and VNO, as well as maintenance of OE neurogenesis during prenatal development. The data suggest a model in which Fgf8 expression defines an anterior morphogenetic center, which is required not only for the sustenance and continued production of primary olfactory (OE and VNO) neural stem and progenitor cells, but also for proper morphogenesis of the entire nasal cavity.
Inducibility of Cytochrome P450 1A1 and Chemical Carcinogenesis by Benzo[a]pyrene in AhR Repressor-deficient Mice Biochemical and Biophysical Research Communications. Jan, 2008 | Pubmed ID: 18022386 AhR repressor (AhRR) is an AhR-related bHLH-PAS transcription factor. It is known to repress AhR transcription activity in a competitive manner. To examine AhRR functions in mice, we produced AhRR-deficient mice by gene knockout. AhRR(-/-) mice were born in normal Mendelian proportions, grew well, and were fertile. AhR(-/-) mice exhibited higher levels of Cyp1a1 (Cytochrome P450 1A1) mRNA induction in the skin, stomach and spleen than wild-type mice, while expression of Cyp1a1 mRNA was not significantly altered in the liver, lung, heart or other tissues, suggesting that "super-induction" of Cyp1a1 mRNA expression in AhRR(-/-) mice occurs in a tissue specific manner. AhRR(-/-) mice displayed a delayed response to skin carcinogenesis caused by benzo[a]pyrene. Since CYP1A1 is involved in the metabolic activation and detoxification of chemical carcinogens, these results suggest that overexpression of CYP1A1 shifts the balance of the metabolic activities in the skin of AhRR(-/-) mice in favor of the detoxification of carcinogens.
Foxg1 Promotes Olfactory Neurogenesis by Antagonizing Gdf11 Development (Cambridge, England). May, 2009 | Pubmed ID: 19297409 Foxg1, a winged-helix transcription factor, promotes the development of anterior neural structures; in mice lacking Foxg1, development of the cerebral hemispheres and olfactory epithelium (OE) is severely reduced. It has been suggested that Foxg1 acts by positively regulating the expression of growth factors, such as Fgf8, which support neurogenesis. However, Foxg1 also binds Smad transcriptional complexes, allowing it to negatively regulate the effects of TGFbeta family ligands. Here, we provide evidence that this latter effect explains much of the ability of Foxg1 to drive neurogenesis in the OE. We show that Foxg1 is expressed in developing OE at the same time as the gene encoding growth differentiation factor 11 (Gdf11), a TGFbeta family member that mediates negative-feedback control of OE neurogenesis. Mutations in Gdf11 rescue, to a considerable degree, the major defects in Foxg1(-/-) OE, including the early, severe loss of neural precursors and olfactory receptor neurons, and the subsequent collapse of both neurogenesis and nasal cavity formation. Rescue is gene-dosage dependent, with loss of even one allele of Gdf11 restoring substantial neurogenesis. Notably, we find no evidence for a disruption of Fgf8 expression in Foxg1(-/-) OE. However, we do observe both a failure of expression of follistatin (Fst), which encodes a secreted Gdf11 antagonist normally expressed in and around OE, and an increase in the expression of Gdf11 itself within the remaining OE in these mutants. Fst expression is rescued in Foxg1(-/-);Gdf11(-/-) and Foxg1(-/-);Gdf11(+/-) mice. These data suggest that the influence of Foxg1 on Gdf11-mediated negative feedback of neurogenesis may be both direct and indirect. In addition, defects in development of the cerebral hemispheres in Foxg1(-/-) mice are not rescued by mutations in Gdf11, nor is Gdf11 expressed at high levels within these structures. Thus, the pro-neurogenic effects of Foxg1 are likely to be mediated through different signaling pathways in different parts of the nervous system.
The Role of Foxg1 in the Development of Neural Stem Cells of the Olfactory Epithelium Annals of the New York Academy of Sciences. Jul, 2009 | Pubmed ID: 19686101 The olfactory epithelium (OE) of the mouse is an excellent model system for studying principles of neural stem cell biology because of its well-defined neuronal lineage and its ability to regenerate throughout life. To approach the molecular mechanisms of stem cell regulation in the OE, we have focused on Foxg1, also known as brain factor 1, which is a member of the Forkhead transcription factor family. Foxg1(-/-) mice show major defects in the OE at birth, suggesting that Foxg1 plays an important role in OE development. We find that Foxg1 is expressed in cells within the basal compartment of the OE, the location where OE stem and progenitor cells are known to reside. Since FoxG1 is known to regulate proliferation of neuronal progenitor cells during telencephalon development, we performed bromodeoxyuridine pulse-chase labeling of Sox2-expressing neural stem cells during primary OE neurogenesis. We found the percentage of Sox2-expressing cells that retained bromodeoxyuridine was twice as high in Foxg1(-/-) OE cells as in the wild type, suggesting that these cells are delayed and/or halted in their development in the absence of Foxg1. Our findings suggest that the proliferation and/or subsequent differentiation of Sox2-expressing neural stem cells in the OE is regulated by Foxg1.
Multiple Organ System Defects and Transcriptional Dysregulation in the Nipbl(+/-) Mouse, a Model of Cornelia De Lange Syndrome PLoS Genetics. Sep, 2009 | Pubmed ID: 19763162 Cornelia de Lange Syndrome (CdLS) is a multi-organ system birth defects disorder linked, in at least half of cases, to heterozygous mutations in the NIPBL gene. In animals and fungi, orthologs of NIPBL regulate cohesin, a complex of proteins that is essential for chromosome cohesion and is also implicated in DNA repair and transcriptional regulation. Mice heterozygous for a gene-trap mutation in Nipbl were produced and exhibited defects characteristic of CdLS, including small size, craniofacial anomalies, microbrachycephaly, heart defects, hearing abnormalities, delayed bone maturation, reduced body fat, behavioral disturbances, and high mortality (75-80%) during the first weeks of life. These phenotypes arose despite a decrease in Nipbl transcript levels of only approximately 30%, implying extreme sensitivity of development to small changes in Nipbl activity. Gene expression profiling demonstrated that Nipbl deficiency leads to modest but significant transcriptional dysregulation of many genes. Expression changes at the protocadherin beta (Pcdhb) locus, as well as at other loci, support the view that NIPBL influences long-range chromosomal regulatory interactions. In addition, evidence is presented that reduced expression of genes involved in adipogenic differentiation may underlie the low amounts of body fat observed both in Nipbl+/- mice and in individuals with CdLS.
Cohesin Mediates Chromatin Interactions That Regulate Mammalian β-globin Expression The Journal of Biological Chemistry. May, 2011 | Pubmed ID: 21454523 The β-globin locus undergoes dynamic chromatin interaction changes in differentiating erythroid cells that are thought to be important for proper globin gene expression. However, the underlying mechanisms are unclear. The CCCTC-binding factor, CTCF, binds to the insulator elements at the 5' and 3' boundaries of the locus, but these sites were shown to be dispensable for globin gene activation. We found that, upon induction of differentiation, cohesin and the cohesin loading factor Nipped-B-like (Nipbl) bind to the locus control region (LCR) at the CTCF insulator and distal enhancer regions as well as at the specific target globin gene that undergoes activation upon differentiation. Nipbl-dependent cohesin binding is critical for long-range chromatin interactions, both between the CTCF insulator elements and between the LCR distal enhancer and the target gene. We show that the latter interaction is important for globin gene expression in vivo and in vitro. Furthermore, the results indicate that such cohesin-mediated chromatin interactions associated with gene regulation are sensitive to the partial reduction of Nipbl caused by heterozygous mutation. This provides the first direct evidence that Nipbl haploinsufficiency affects cohesin-mediated chromatin interactions and gene expression. Our results reveal that dynamic Nipbl/cohesin binding is critical for developmental chromatin organization and the gene activation function of the LCR in mammalian cells.
Reduction of Nipbl Impairs Cohesin Loading Locally and Affects Transcription but Not Cohesion-dependent Functions in a Mouse Model of Cornelia De Lange Syndrome Biochimica Et Biophysica Acta. Dec, 2013 | Pubmed ID: 23920377 Cornelia de Lange Syndrome (CdLS) is a genetic disorder linked to mutations in cohesin and its regulators. To date, it is unclear which function of cohesin is more relevant to the pathology of the syndrome. A mouse heterozygous for the gene encoding the cohesin loader Nipbl recapitulates many features of CdLS. We have carefully examined Nipbl deficient cells and here report that they have robust cohesion all along the chromosome. DNA replication, DNA repair and chromosome segregation are carried out efficiently in these cells. While bulk cohesin loading is unperturbed, binding to certain promoters such as the Protocadherin genes in brain is notably affected and alters gene expression. These results provide further support for the idea that developmental defects in CdLS are caused by deregulated transcription and not by malfunction of cohesion-related processes.


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PHYLLIS: This is someone who very detail – oriented in life and the simplest things that they have done over the years began to slip. Was unbelievable. Years ago, you know, maybe we can just keep a closer eye on things need. And it was a little shakeup in her life and that's when we noticed a stronger appearance – a steeper decline of Alzheimer's progression – and she had to go to the doctor when we two months ago two months ago. But we discovered, anywhere from three to four years.
JOE: My recollection of the first time I had felt anything with my wife, when we sold our home of 50 years and moving with our daughter. She reacted strangely and I thought it was due to the movement. But later, I thought not not able to remember things – our house of 50 years, she could not remember. They went in different tunings. Continue reading
The ability to absorb the institution and to process salt can manifest themselves in higher or lower blood pressure.Tavistock group has searched worldwide for patients with very high or very low blood pressure caused by mutations in single genes. Such patients are often identified through family stories extreme blood pressure. To date, his group gene mutations gene mutations that lower or increase blood pressure, including those found in the extremely low blood pressure in patients with Gitelman and Bartter syndromes found cause..
– 'The results show that nearly 2 % of the FHS cohort a defective copy one of these three genes has,'Lifton said. 'In contrast to patients with Gitelman and Bartter syndromes, these subjects have only one defective copy, not two. '.. By sequencing each of the three genes of DNA samples obtained from 3125 participants in the Framingham Heart Study, and this additional biochemical, genetic and genomic analysis found the HHMI team sampled functional mutations in one of the genes in at least 1 in every 64 of the study participants. Continue reading
The feminist movement in the 20th Century saw medical treatment in relation to women, the legal and personal rights over their own body. The Warwick seminar governance looks more like the latest biomedical trends affecting their self-esteem.
The British Sociological Association 's mission is to represent the intellectual and sociological interests of its members. Rch analysis in Nature explains wide variations in animal sleep habitsA comprehensive research analysis by a neuroscientist at the UCLA Semel Institute and the Veterans Affairs ' Neurobiology Research Laboratory concludes that the environment and diet largely determine needs sleep. Dan Page University of California – Los Angeles newsroom. Continue reading
The report is in the latest issue of The Cochrane Library, a publication of The Cochrane Collaboration, an international organization that evaluates medical research published. Lead author Sally Merry, a pediatric psychiatrist with the department of psychological medicine at the University of Auckland in New Zealand, said:.. In India, AIDS brings with it a huge social stigma. About 4.5 million people are infected in India.
AIDS in Asia spreading at an alarming rate, the conference organizers would like to highlight this problem at the conference. Heads of India and China were invited There is no confirmation yet that they get AIDS experts are concerned. That the AIDS crisis in China and India could be as bad as the African epidemic, if no action is taken soon. Continue reading
– What is most interesting is to see what has happened to baby boomers in midlife, says Whitbourne. Some were more satisfied, others were desperate, and yet others remained relatively stable My research design me to assert allows the changes in their lives were most closely associated with a growth in fulfillment.. The study began in 1966 at the University of Rochester in New York, when a group of students participated in a research project on personality development. Through life.ies of successive generations of students at Rochester and follow-up surveys with the participants in the earliest groups have experienced 34 years information about the changes in the lives of leading edge boomers who were indulged in their mid to late 50s and the trailing edge edge boomers, in their in their mid-40s at the time the last survey.
They have a sense of connection to younger generations and social conscience. .. Me Generation ' Baby Boomers will find fulfillment through volunteer, Family Tiescan baby boomers represented in the vernacular as whiners, complainers and narcissists, but a new study from the University of Massachusetts Amherst psychology professor Susan Krauss Whitbourne, the 50 – year-old says are always a bad rap. – It is wrong to say that baby boomers are selfish and only care to stay young, says Susan Krauss Whitbourne. Continue reading
This transplanted myoendothelial cells the injured muscle repair stimulates the growth of new blood vessels in the heart and reduced scar tissue from the injury, thereby dramatically improving the function of the injured left ventricle, said Dr. Head of the Stem Cell Research Center at the Children's Hospital John G sildenafil dose for erectile dysfunction . Rangos Sr. Research Center. This study is our belief that this novel population of stem cells discovered in our laboratory tremendous promise for the future of regenerative medicine holds Specifically confirms myoendothelial cell potential as a treatment for show suffered myocardial infarction show, 'said Dr. Huard, the Henry J. Mankin Professor and for research in for research in the Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine. 'the main benefit of our approach is that a therapy, it would be an autologous transplant. This means that for a patient who suffers a heart attack, we would muscle biopsy muscle biopsy from his muscles, isolating and purifying the cells and again myoendothelial they inject into the injured cardiac muscle, whereby. The risk of rejection by the introduction of foreign cells '.
###Dr. Huard and colleagues in the Stem Cell explore explore developing numerous therapeutic uses for the population of muscle stem cells, the team identifies. One of the most promising uses could for the treatment of Duchenne muscular dystrophy , one genetic disease that affects a boys of 3500. Patients with DMD lack dystrophin, a protein, muscle structure gives. Huard is an internationally recognized cell biologist conduct laboratory research into the therapeutic use of stem cells of of musculoskeletal and orthopedic diseases and injuries. In the lab, Dr. Huard is developing cutting-edge therapies for bone, Regenerate cartilage and peripheral nerve and skeletal muscle damage after exercise and military violations repaired. Continue reading
The pace at which published published with the Genome Sequencer System their discoveries demonstrates the high data quality and easy analysis of 454 Sequencing results. – 'Reaching of our 250th peer-reviewed publication so quickly, demonstrates the ease with which researchers can interpret 454 sequence data and make biologically meaningful conclusions,'explained Christopher McLeod, President and CEO of 454 Life Sciences. 'The combination of 454 Life Sciences ' long read of of easy – to-use analysis tools means our customers can the the science, technology.
The researchers estimate that approximately 30 million men will have difficulties in finding a partner in China as a consequence of the lack of female adults. Marriage, which of this situation is due to the gender imbalance between the births that take place, although there are also other reasons, such as the migration of women from the poorest rural areas for other richer regions. 'This has has created a huge market for the sale and trafficking of women,'conclude Quanbao Jiang and Isaiah? Javier S S? 'The trafficking of women is virtually nonexistent in China since 1949, but we have found that this crime is on the rise since 1980,'added Professor S?. Continue reading
The most common side effect after treatment mild reactions at the injection site was.Because of the increasing interest in cancer vaccines, Gulley recommended for further studies carried out to determine which vaccines are to be advantageous for certain patients erectyle disfunction .He concludes:'Continued use in some patients in this study highlights the potential for therapeutic vaccines to impact clinical outcomes without toxicity but further studies in the respective patient groups are needed to adequately assess efficacy.
'With this vaccine, we can clearly generate immune responses clinical responses clinical responses in some patients. 'the study, which involved the National Cancer Institute, Gulley and his team of 26 patients, all of them heavily pretreated, with 21 patients who have at least three prior chemotherapy treatments, funding and called them the monthly vaccinations PANVAC vaccine obtained that transgenes containing MUC-1, CEA and three T-cell costimulatory molecules. Continue reading
' Nobody ' is assigned to the patient care problems on 2nd Comprehensive CDPH Complaint Filed to investigate to investigate, after months of efforts by UC Davis RNs and CNA hospital officials products, understaffing, which is the source of most correct, the patient care issues. But despite several follow-up calls in recent months , the state has not been studied. 'No one has been assigned, 'a CDPH representative CNA UC Division Director Beth Kean.
In Davis, a third of the films were were RNs with less than the legally required based on how busy occupied as revealed by the hospital 's own documents.As a result, individual patients at UC Davis at UC Davis at risk because of the short set staffing, according to the complaint. Some examples:. Continue reading
'courtesy of you, the entire Kaiser Daily Health Policy Report view, search the archives, or sign up for email delivery at Kaiser Daily Health Policy Report strongly supports kaiser network. J. Free service of The Henry J. Releases. Kaiser Family Foundation. 2005 Advisory Board Company and Kaiser Family Foundation. All rights reserved.
On the 29th January, the Peanut Corporation of America an expanded voluntary recall of all peanuts and peanut products in its Blakely, Georgia facility since 1 Processed January 2007. The expanded recall includes all peanuts , granulated peanuts, peanut meal, peanut butter and peanut paste. From the industry. By the recall peanuts and peanut products were made only at the company Blakely, Georgia plant. Continue reading
All trademarks mentioned in this release proprietary.References1) Marcellin P et al. HBsAg clearance continues to increase after the end of treatment with PEGASYS? Asia-Pacific 5-year follow-up study in patients with HBeAg-negative disease. Presented at: Asia-Pacific Association for the Study of the Liver , 13 to 16 February 2009; Hong Kong.
Begin all patients with e-positive disease. For e-positive patients, loss of the e-antigen after treatment, or e-seroconversion ', that therapy has worked well, and is a first important indicator of treatment success. In a new study, the e-positive patients, the results showed that 50 percent of patients whose s – antigen levels significantly decreased 24 weeks after initiation of treatment with Pegasys could be e-seroconversion ', an important treatment option endpoint for these patients. Achieve In addition, approximately 20 percent of patients with e – seroconversion went on S-antigen, a so-called clinical medicine after after treatment had ended achieve. Continue reading
Real timeLife Technologies Receives FDA marketing approval for Flow Coupler Synovis Life Technologies has Food and Drug Administration 510 approval to market its flow pair, established an innovative extension the received company microvascular anastomoses Coupler used in several surgical procedures to connect small blood vessels more quickly and with equal or greater reliability than hand sewing. The flow coupler features Doppler technology to immediately measure blood flow after connecting the small blood vessels.
Synovis ' original Coupler is widely used in microvascular surgery, including breast reconstruction in cancer patients as well in several applications in head, neck and hand reconstruction. The device provides a patency rate than that of the hand stitching required when connecting vessels in approximately one fifth of the time of hand sewing aschemic aschemic shorter time for the transplanted tissue, significant time savings in the operating costs and savings what. Continue reading
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Elected members of the International Association of Machinists and Aerospace Workers, who test and launch Delta rockets in Southern California and Florida to hold a strike after they rejected a final contract offer from Boeing because of reduced health benefits. According to the Times, the contract offer $ 3,000 $ 3,000 lump-sum payment in the first year, a 2 percent wage increase in the second year and a 2.5 percent wage increase in the third year of their providers, and would have to pay staff required additional out-of-pocket heath care costs. In addition, the contract proposal would eliminate retiree health benefits for new hires, the Times reports. About 25 percent United Auto Workers members begin on Thursday on a tentative agreement with GM would be union retirees to pay monthly health insurance premiums and annual deductibles reduce company healthcare costs, the AP / Kansas vote City Star reports Durbin, . Continue reading
But the researchers also properties of the TRPA1 channel has determined that account for his alleged role in parallel does not go away pain sensation, why in some cases, pain from an injury, as long as the violation remains.
TRPA1 is known to be generated by the pain chemicals such as the pungent components of edibles such as wasabi, horseradish, cinnamon and Listerine , which is why they sting the mouth, declared to be activated. Continue reading
Daniel A. Director of the Massachusetts General Hospital Cancer Center, Director, Center for Cancer Risk Analysis and physician, Massachusetts General Hospital, Laurel Schwartz Professor of Oncology and Professor of Medicine at Harvard Medical School, Program in Cancer Genetics, Dana Farber – Harvard Comprehensive Cancer Center, Mass.
Four new members were elected to the AACR nomination Committee for the 2007-2009. They will serve in addition to the four current the the candidates slate for president-elect and director to develop. With the Board of Directors, they will select the candidates for the next nominating committee slate. The newly elected members are:. Continue reading

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Tomorrow is Heritage Day in South Africa. If I were to celebrate my heritage what would it be? I suppose at first glance you might classify me as a WASP (White Anglo Saxon Protestant) yet that doesn't describe the unusualness of my upbringing – raised in Northern Ireland in the protestant community, but raised to be non-political and non-religious. My mum thought it best to bring us up with the freedom to choose for ourselves – in the end we all choose Jesus. You see God had a secret weapon in our family – a praying grandmother. While my Dad's mum didn't live to see the whole family come to faith she was around to see me take my first steps – she got to attend my baptism shortly before she died. What she didn't see was my parents and brother also come to faith over the following years. I discovered that this quiet, hair-in-a-bun lady, whom I didn't know very well was a prayer – over many years she prayed with her best friends for our family's salvation.
My mum gave me a heritage of freedom and acceptance of different people and the freedom not to be confined by a culture or tradition. My grandmother gave me a heritage of faith – the freedom to believe. What heritage will I pass along? What will be my legacy? I hope that I combine the heritage of two ladies who so often misunderstood each other. The freedom to celebrate different cultures and the liberty not to be confined by them – a kind of best of Malawian, Northern Irish, South African and anywhere-else-we-stop-along-the-way culture.
Revelation 7:9-10 After this I looked and there before me was a great multitude that no one could count, from every nation, tribe, people and language, standing before the throne and in front of the Lamb. They were wearing white robes and were holding palm branches in their hands. 10 And they cried out in a loud voice: "Salvation belongs to our God, who sits on the throne, and to the Lamb."
God recognises and celebrates our cultures – our differences do not need to be divisive. But underpinning all our cultural diversity is our common faith and common saviour. I want Jesus and obedience to God to be my lasting legacy.
Psalms 119:111-112 Your statutes are my heritage forever; they are the joy of my heart. 112 My heart is set on keeping your decrees to the very end.
I pray that the heritage I pass on is a combination of what I received – a celebration of freedom and the acceptance of Jesus. That I would be as accepting of people and cultures as Jesus is, but also that the law that I adhere to would not be dictated and limited by culture but by God's eternal standards. What is you heritage?


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MP Board 12th Result 2015 details is Published on This Page. You can get all the related information regarding MP Board Result from here. You can also register yourself in our portal for the updated notification regarding the result on your mobile and Email.
MP Board 12th Result 2015 finally releasing on May 10, 2015 at 4 pm evening at official portal of MP Board on www.mpresults.nic.in
Check MPBSE MP Board 12th Result 2015 at mpresults.nic.in
- Open the official website, www.mpresults.nic.in
- Click on the link "Class XIIth (MP Board) 2015 Results".
- Enter your roll number or name.
- Click on submit.
- Take a printout of your result.
MPBSE MP Board 12th Result 2015:
MP Board 12th Result 2015 is to be announced by Madhya Pradesh Board of Secondary Education also known as MPBSE or MP Board tomorrow on May 10, 2015 at 4 pm evening at official website mpresults.nic.in. MPBSE has issued the official notification yesterday on their official portal to inform the students regarding their result. Though there was no notification till yesterday evening so it was expected that the MP Board 12th result 2015 will be announced in the mid month of May.
Madhya Pradesh Board of Secondary Examination, Bhopal (MPBSE) is a Madhya Pradesh Government body responsible for determining the policy-related, administrative, cognitive, intellectual direction of the state's higher educational system. The Board regulates and supervises the system of Intermediate education in Madhya Pradesh State. Madhya Pradesh Board is responsible for conducting High school and Intermediate Examinations. In addition, the board is also involved in recognizing new schools, inspection of the schools that are recognized by the board and also evaluate their performance.
MPBSE every year conducts the examination for Class 10th and Class 12th students and provides better education in the state of Madhya Pradesh. This year the examination were conducted from March 2 to April 7, 2015. After the completion of exams answer sheets checking were started. As compare to last year this year the result is releasing a week before. Students started searching about their MP Board 12th Result 2015 on net but they were not getting the correct information regarding their result publication date. Now candidates can visit the official website and can look for all sorts of information regarding the result. After the declaration of result students can make print out of their result and can keep with them till they get original from their respective schools.
Check MPBSE MP Board Result 2015 Here:
Check MPBSE MP Board 12th Result 2015 Name wise
The Madhya Pradesh Class 12 Examination is called the MP HSSC Examination and it is conducted by the Madhya Pradesh Board of Secondary Education(MPBSE) Bhopal. Last year 11 lakh candidates appear for 12th examination and this year in 2015 around 12 lac of students appeared and waiting for their result which is to be published with in coming 24 hours. The Class 12 examination is important exam in the life of students. This result help students in moving forward towards their career where they can do their UG/PG level studies. MP Board Class 12 Result 2015 is scheduled on May 10, 2015 at 4 PM.
MP board also provides the facility for the students to check their result in name wise format also. We will be providing the information which will help you all in getting your result in name wise format. For this students are advised to enter their Name and their father name along with correct registration number provided by school to the students correctly. This access of result will not take any time and will easily provide the result on their desktop screen. MP Board 12th result 2015 of different streams like Science, Commerce and Arts will be declared on the same day.
If you have any queries regarding MP Board 12th Result 2015 name wise you can contact on the given contact details, candidates can leave their query as a comment here too. Students are advised to stay tuned to our website for updated information related to MP Board Result 2015
MP Board of Secondary Education,
Shivaji Nagar, Bhopal- 462011
Phone no: 0755- 2551166-71
Fax: 0755- 2552061
Website: www.mpbse.nic.in
Email: firstname.lastname@example.com


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Mandalay Cycling Holiday .This proposed biking itinerary starts directly from the airport of Mandalay on your flight arrival from Yangon or Pick you up from your hotel in Mandalay. Mostly domestic flight arrive at Mandalay Airport in the morning time around 0845am. International flight used to arrive at Mandalay Airport around 1230pm. Our guide will wait you at the Mandalay International Airport and start explore Mandalay with biking tour. Tour includes some major cultural sites of interest as Ava (Inwa), Amarapura, Sagaing and Mingun along the Irrawaddy River during 2 day trip.
Day 01: Mandalay- Amarapura-Ava-Mandalay (with car & bike)(L)
8:00 Am Meet and greeting by our biking guide from your hotel and start briefing for a while. Then cycling tour starts from Mandalay to Tadar Oo Town with a first stop for refreshment (10 miles/16 Km).
After a short stop, you will leave from Tadar Oo and continue to Myit Nge Bridge (3 miles/5 Km), then pass Myint Nge Bridge and continue to U Bein teak bridge (10 miles / 16 Km) for a second stop.
You will visit 150-year-old U Bein teak bridge, built in 1851 at the time when Amarapura was the royal capital. Lunch at a local restaurant in Amarapura.
After leaving U Bein teak bridge, you will continue to Oe Htoke Tan Jetty (5 miles/8 Km) and take the local ferry to Innwa (Ava), located on an island between the Ayeyarwady and the Myit Nge Rivers or we will continue cycling through Ava bridge and touring around the Ava (Innwa). Visit Nan Myint Watch tower, Menu Oat Kyaung.
After that continue cycling back to Mandalay and visit to Mahamuni Pagoda, Kutodaw Pagoda, Golden Palace Monastery. Evening visit to Mandalay Hill to enjoy panoramic sunset view over the Mandalay Hill. Then transfer back to your hotel . Overnight at the hotel in Mandalay.
(Cycling distance- 60km with flat road)
Day 02: Mandalay-Sagaing- Mingun-Mandalay (car, bike, boat)( L)
Breakfast at your hotel 8:00 Am we will be start cycling around Mandalay. Visit Zay Cho Market and touring around Mandalay to visit handicrafts. After that, ride will continue to Sagaing Bridge passing over the Bridge to Sagaing, which is perhaps the living centre of Buddhist faith in Myanmar today . Your ride will then bring you to Let Pan Village (10 miles/16 Km) for a stop and some refreshment. After refreshment, you will visit a nails workshop family. Lunch at a local restaurant. After that, you will continue cycling to Mingun for a last stop along the bank of Ayeyarwaddy River (4 miles/6 Km) and will Pahtoedawgyi Pagoda, and Mingun Bell, the world's largest intact bell. Then visit Home for the Aged at Mingun Hill. Late afternoon, after sightseeing, you will cross the Ayeyarwaddy River by local ferry to Mandalay and enjoy the sunset. The boat trip will give you the opportunity to discover the river life with different type of cargo or passenger boats but also bamboo rafts. Then arrive at the Jetty we will continue ride to your hotel. And then we will dropped you at your hotel.
(End of Tour Program)
Daily Departure from your hotel in Mandalay
Contact us for good price
Tour Inclusive :
* Support transport by A/C mini van, bus, truck
* Trek & Giant MTB, helmets, spare parts, aid kits, tool kits
* Entrance fees
* 02 L=Lunch as mentioned in the tour itinerary
* For daily cycling only: Water, snacks, fresh fruits, wet towels
* Experienced English speaking bike guides
Tour Exclusive :
* Travel insurance
* Accommodation in Mandalay
* Meals not in tour
* Other personal expenses
* Drinks at restaurants and tips for guide


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News Intern - Summer 2015
Elise Cruz is focusing on community design, land use, and transportation. She is currently enrolled at UVA and is entering her final year of the Masters of Urban and Environmental Planning program where she also serves as the President of the Student Planners Association. She is passionate about healthy communities, social justice, and placemaking. A Wisconsin native, she is enjoying the Charlottesville weather patterns and is wondering where all the snow is! She enjoys playing the violin, biking, practicing calligraphy, and cooking.
What makes Charlottesville-Albemarle special to you?
I most recently lived in Ashland, Wisconsin on the shores of Lake Superior. I need big geographical features to keep me grounded and humble. The Blue Ridge Mountains drew me here and will keep me here for a while. I love the rich history of the area: evident in the music, food, architecture, and the locals themselves. I enjoy exploring all corners of the city and county to find the best glass of wine or a new favorite reading spot.
Ultimate Charlottesville Saturday?
My ultimate Charlottesville Saturday would start with a drive down to Bellair Farm to pick up my CSA and grab some strawberries and flowers from the pick-your-own fields. I'd mosey back into town with a quick stop for a BBQ sandwich. After a nap, hitting a local trail for a hike sounds perfect. The day would be capped with live bluegrass and a local brew.
Most look-forward-to part of working at Charlottesville Tomorrow?
I am looking forward to gaining a new perspective on the Charlottesville-Albemarle community and getting to know its residents and leaders. Having a "sense of place" has always been important to me. I am eager to see how this internship changes my understanding of this place and my feelings of belonging here.


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Introducing Real Talk, The Show That Celebrates You For Being, Not Doing
Happy MLK day, people! I hope you've enjoyed this day off, and are rocking your Monday. While I have your attention, I wanted to take this moment to unveil a project I've been working on for a bit and am SUPER excited about. Super excited.
Introducing... "Real Talk, the show that celebrates you for being, not doing."
Yup. I'm starting a talk show, YEEHAW! A next-gen online talk show series of sorts. Ya dig?
And, I'm interviewing you. And you. And you and you and you andyouandyouandyou. All of yous.
Let me explain.
I figured, everyone's always interviewing people and celebs and influencers for what they've DONE in life and how many Instagram followers they GOT for what they do and the job titles for what they DO
And I'm totally down for that because a lot of cool people are doing a lot of cool sh*t in this world and we need to tell people about it. For sure.
But I think EVERYONE has a story, REGARDLESS of what they DO or how many Instagram followers they GOT or what job title they HAVE.
I want to give the every woman a platform for who she is BEING. I want to help people tell THAT story. Their story. The story of who they ARE.
Celebrate them for BEING, not just DOING. And so I'll be asking some real questions to get to the heart of who people are. But first! I've answered those questions myself. Check that out above. Gotta give what you wanna get, right?
This is our debut, and I'm asking that you, you darling reader you, get involved. I want to know YOUR story, who YOU are.
Consider this a call for video submissions! We want you to be a part of this next-gen online talk show.
We want to Real Talk with you.
Here's how in 3 easy steps:
1. Video yourself answering these interview questions:
- Who or what has made you who you are?
- What lasting effect do you want to have on this world?
- When was the last time you cried?
- If you had a moment of reckless honesty, what would it be?
- Describe your life as a movie. (Give us the 30-second elevator pitch).
- How do you want to be remembered?
- If you didn't have to worry about money, how would you spend your time?
- List 3 words that describe who you are NOW and 3 words that describe who you WANT to be.
- When was the last time you felt the 3 words you WANT to be?
- AND ask ME a question, too, at the end!
2. If you can keep your video to UNDER 5 minutes, AMAZING! (Please try to get as close to that as possible.)
3. Upload your Real Talk segment to YouTube and Tweet us the link @selfloveformula with #RealTalkwithJFC
And BOOM, done.
We'll review your segment submission and, if accepted, we'll let you know when it goes live on our site, TheSelfLoveFormula.com.
Thank you SO much for watching and reading and recording and participating and sharing!
Jams: Be Love (Kris Roche) / CC BY-NC-SA 4.0


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Stephen Moyer Starring in "The Big Valley"
July 3, 2010 by Patty Lindsell
With shooting for Season 3 of True Blood just finished (we hear the blooper reel at the wrap party was a scream) cast members will be off working on side projects during hiatus.
For Stephen Moyer one these projects will see him transported from the steamy, dark nights of Louisiana (filmed in California) to the sunlit, sweeping vistas of California (filmed in Louisiana – go figure).
Stephen will be starring in the big screen remake of the 1960s TV Western, "The Big Valley". "The Big Valley" follows the travails of ranchers in California's San Joaquin Valley in their battles with the Southern Pacific Railroad during the 1870s.
Stephen will take on the role of Jarrod, the eldest son of ranch matriarch Victoria Barkley (Jessica Lange). In the original series Jarrod is described on Wikipedia as "educated, refined … and handled all of the family's legal and business affairs" (Shoot! So the chances of seeing Stephen in chaps are not so good.)
Also starring in "The Big Valley" will be Lee Majors (who played Heath in the TV series); Bruce Dern, Richard Dreyfuss, Lorraine Nicholson (as Audra) and Jason Alan Smith (as Nick).
Alongside Stephen will be Travis Fimmel, his former co-star on the Aussie flick "Restraint" (2008), who will play Heath.
Production on "The Big Valley" is due to begin next month.
(Photo credit: thecinemasource.com)


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By Veeva Systems
Published: May 2017
Discover how a cloud-based clinical trial management system (CTMS) brings together documents and data to better support today's complex clinical trials, where legacy CTMS applications fall short.
To learn more register for Veeva Systems' free white paper Is your CTMS working for you?.
Veeva Systems Inc. is a leader in cloud-based software for the global life sciences industry. Committed to streamlining the end-to-end clinical trial process, Veeva offers the industry's first cloud platform that combines EDC, eSource, CTMS, eTMF, and study start-up to unify clinical data management and clinical operations. Driven by innovation, product excellence, and customer success, Veeva has more than 450 customers, ranging from the world's largest pharmaceutical companies to emerging biotechs. For more information, visit http://www.veeva.com.
Copyright © 1995-2018 CenterWatch.
Clinical Trials in thePrecision Medicine Era:
A WCG | CenterWatch FREE Virtual Roundtable
Thursday, Aug. 23, 2018 • 1:30 p.m. - 3:00 p.m. EDT


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Build Guide by Center
Not Updated For Current Season
This guide has not yet been updated for the current season. Please keep this in mind while reading. You can see the most recently updated guides on the browse guides page.
Thank You!
Your votes and comments encourage our guide authors to continue
creating helpful guides for the League of Legends community.
Katarina Build
Ability Sequence
Not Updated For Current Season
The masteries shown here are not yet updated for the current season, the guide author needs to set up the new masteries. As such, they will be different than the masteries you see in-game.
Katarina, an Introduction
Katarina is a high damage character with minimal defense. She focuses oh killing the opponent before they can kill her, rather than trying to outlast them. While being surprised by a group of enemy heroes, or incapacitated in their presence, will often result in death, Katarina is quite capable of removing an entire team in the right situation.
Katarina, With Knives is an AD build. This means that Katarina focuses on increasing her Attack Damage (AD) throughout the game instead of her Ability Power (an AP builds choice). Although Katarina is increasing attack damage, all damage from abilities will be magic damage. Also, despite boosting her attack damage, Katarina rarely whacks enemies with regular attacks.
I have found that an AD Kat hits significantly harder than the AP equivalent, has more ability to harass and damage groups of enemies, and is better at killing minions.
An AP Kat has shorter cooldowns.
To other Katarina publishers:
"Hey! This build looks like mine" you think, "I picked the same skills..and waited to get death lotus as level 6 too! Why, we even picked some of the same weapons!"
Sadly, this is likely due to the limited choices when constructing a build rather than a nefarious plot I conceived. Most Kat builds end up with 5 points in Bouncing Blade, 5 in Killer Instinct, 5 in Shunpo and 3 in Death Lotus. There are the rare builds: 4/4/4/6, increasing Death Lotus at the cost of other skills; the famous 0/5/5/1 that focuses on one, and only one, target; but most builds end up in the same place skill wise. And I feel confident that my decision not to prioritize mana regeneration was an original thought. I definitely stole the idea of buying B.F. swords from you though.
You, Spells
Teleport - sometimes you need to be somewhere else. Youre base to their base in 4 seconds, or theirs to yours. Teleport to that turret on the top before they can finish it off, or to where those two heroes are pressing a little hard despite their low health cause they think they are alone. No spell can compare to the utility of being in the right place at the right time.
Flash - Perfect for sneaking forward for that last hit before he gets to safe ground, or getting away before that turret's shot can kill you. Flash closer to a minion so that you can shunpo to it, move a screen away from pursuers in a heart beat, or just hoping to the other side of an obstacle.
In case you couldn't guess, teleport and flash are my skills of choice.
Ignite - It does damage and slows healing, but if Kat is in range for ignite, she is in range for her other attacks. Ive certainly seen enemies escape with just a little health, but I think teleport and flash both get Kat more kills (to say nothing of flash keeping Kat alive) than ignite would.
Cleanse - The main problem isn't being stunned, its that being stunned stops your channeling. If you're stunned in a group of enemies, you are dead. If its just one enemy, you can probably get away. Either way, that one second less stunned makes little difference.
(since stunned is the most annoying thing that cleanse fixes, I used it when discusses Cleanse)
Exhaust/Ghost - These make chasing and escaping easier, but just dont fit my style. I prefer, flash jumping in and out of range instantly, rather than the speed increase from ghost. Typically I just need to get into range for one last shunpo/blade combo. Like Ignite, Exhaust requires you be in range with Kat's other skills to cast. I would rather rely on the rest of my team to slow than waste an spell on it. It also only targets one enemy, so you are only able to slow one hero Death Lotus is targeting.
You, Runes
Offense! Magic Penetration! Nothing as fun as hitting harder. Unfortunately, seals don't come in the magic penetration variety. They do come with attack damage, but at less than 1 damage per seal at level 18, I cant bring myself to use them. So, Katarina decided to dodge. Combined with the defensive mastery she ends up with around an 8% chance to run a little faster after someone attacks her. Not good by any means, but I don't see a better option.
Katarina, Masteries
Offense! Sorcery, Archaic Sorcery and Havoc, for cooldowns, damage, and damage respectively. If you dont understand why these are my choices, the skill section should clear it up.
After that, it doesn't matter much. I went with defense, picking the ability to run faster after dodging because it could, conceivably help, but I have no real attachment to it. If you like the utility tree, or different defense skills, or even offense, go ahead and try them out, see what works for your style of play.
Katarina, Skills
Bouncing Blade - As an AD build, this will be your most used skill. Kat tosses out a blade that bounces around hitting enemies, dealing damage equal to your attack damage plus a little magic damage. At level 1, it bounces 1 time, hitting 2 targets. At level 5 it bounces 5 times, hitting 6 targets. Unfortunately, it cannot return to a target as Mounsieur Fiddlesticks' crows can, but it remains a very potent skill none the less. If you know an enemy is lurking outside of your range (or vision) and see a line of minions charging towards you, toss your blade at the front on. At higher levels the blade will travel up the minions line, and has a decent change at bouncing towards the cowardly hero, killing (not very likely, atleast more than once, most people learn quickly not to hang around) or driving the hero back to the base.
Kat's manaless aspect and the blades low cooldown means that she should be tossing it out most of the time, there's no need to be shy. She is almost a ranged character, it can be used to easily kill minions while staying out of an opponents range.
Even at end game when everyone is at level 18, this can take out a major chunk of Annie's or Ashe's hit points, even after four or five hits. If you find an occasion to hit their gathered team with it, this can easy do two thousand damage split over the team, more if they are willing to wait a few seconds for you to toss it out again.
Killer Instincts - When activated before another skill, makes the another skill better. Bouncing Blade will hit for full damage on all hits instead of losing 10% per bounce, and half healing for a few seconds after connecting. Shunpo (the next skill) will give 2 seconds of damage reduction, peaking at 35% of damage reduced. Ill talk about it a bit more then. Because the bonus to Bouncing Blade does not increase with level, and that my most common use for Killer Instincts, this skill is left at level one for as long as possible.
It gives bonus damage to abilities and melee attacks too, even if not activated!
Shunpo - Teleport to an enemy! Deal damage to an enemy! Reduce damage from an enemy! All with a short cooldown! Wonderful, right? No follow-up saying it isn't, it really is a wonderful skill. You can close with enemies in a heartbeat, at level 2, with one point in the blade and one in this, you do ~200 magic damage...and be ready to do it again in 10 seconds. This is, in case you were wondering, is what an AP Kat relies on instead of bouncing blade. The only flaw with this skill is that it teleports you to the unit you use it on, and sometimes that is simply not something you want. So, before attacking with it, think, "Is it worth hitting that guy if its going to move me under that turret, face to face with 3 enemy heroes?" The answer may be yes, but probably is no.
Fear not! There are many times when it is incredible useful. Chasing an enemy hero who is just a bit faster than you? Don't worry, you just need to keep him in range of shunpo and you can easily catch up! Got a little excited and overextended, and now you've got death incarnate breathing down your neck? Shunpo to one of your units! (Kat must shunpo to a unit, not a space, so this is difficult to work outside of the lanes where minions are cute little shunpo targets.
Bouncing blade on cooldown and theres a minion just begging to die? Use shunpo to slay it! You have enough mana you can afford to use it on minions!
Death Lotus - Spin in place, channeling, encouraging tiny little daggers to shoot out and hit enemy heroes. It has a cool down of a minute. At level 1, Death Lotus targets 1 enemy hero (unfortunately, deciding who is left to higher beings); at level 2, 2 enemies, and level 3; 3 evil heroes. I would guess she tosses two daggers per second, per target. At level 1, each dagger does fifty plus either fifty-five percent of you're bonus attack damage or thirty-five percent of you're ability power, whichever is higher (50+ 55% of AD, or 50 + 35% of AP). At level 3, the base amount jumps to 80, but the bonuses stay the same.
Not surprisingly, this is you're main killing ability. Get into a group of enemies and start twirling and they will get hit hard, fast. The result of this is that most teams will make a point of stopping you faster, which can be done with silence, stun, knock up, knock back, and fear. And probably a few other things, its not that hard to stop. Enemies can also escape the range of Death Lotus fairly easily by running away from you, the ability does not have a wide radius.
But it hits really hard.
Your main task as Katarina is simple. Get the most out of Death Lotus. Once you get it, 70% of what you do will be trying to set up death lotus. Ten percent will be harassing enemies before a big fight, ten percent will be chasing enemies after a big fight, and ten percent will be running away as fast as you're little legs can carry you. This is a time when shunpo shines. Pick out the best place and use shunpo to get there as quickly as you can. Wait until any heroes that can stop you are waiting on a cooldown or otherwise engaged. If someone can stun or hold the other team in place so that you can get them in Death Lotus for as long as possible, that's fantastic too. If you can, sneak behind them in a team fight, wait until they are all fighting in a big melee, and teleport into the middle and hit Death Lotus, you can easy devastate an enemy team.
Unfortunatly, this does mean you will be the focus of their attention, and as a shy hero, Katarina does not thrive when so many are focusing on her.
Voracity - Katarina's passive ability, she mysteriously receives 50 Gold and 15 seconds off her cooldowns when she contributes to the death of an enemy hero (With a kill or assist). This is an excellent ability, and in case anyone had any doubts about what she is good at, it makes it quite clear. Katarina should focus on being part of every kill she can. All abilities but Death Lotus will be fully refreshed when she gets a kill (or assist); this means that when chasing fleeing enemies she can shunpo and bouncing blade endlessly if she kills one every time she fires the combo. It also means she will be ready for all conflicts. If you sneak up behind an enemy and Death Lotus, killing them, you'll only have around 40 seconds left on Lotus' cool down. This passive ability makes Katarina's already spammable active abilities even more spammable.
And more gold.
Katarina, Items
Katarina should always focus on maximizing her Death Lotus damage. This build chooses to do it by focusing on attack damage. Therefore, attack damage items are the best choices. However, before you can do that, boots are necessary. This game is largely about mobility, iIts almost impossible to escape or kill a character who is faster than you. Boots are the easiest, fastest, way to increase it. And you get to upgrade yours to pierce magic damage!
So, I recommend you begin with a potion or three (you will get hit, that's good, it means you are playing) and boots.
After that you have more options. While fighting, get an idea for how good your opponents are. Glance at other lanes, see if they are pushing with low health. If, after glancing around, it looks like you're going to be able to get kills without that much trouble, consider getting Sword of the Occult. With 20 stacks (10 kill, or 20 assists, or some combination) it gives 100 attack damage (as much as any item) and 15% increase to speed. However, if you doubt your ability to feed it fully, don't get it, at least not now. The other item you should look at is Sorcerers Shoes, a fantastic increase for your boots. They lower magic penetration by 20%, making all you're abilities hit harder. If you need some more potions, don't be shy about buying them! They are cheap though they do give you less and less health (relative to you're total health) as the game goes by. A potion healing 200 health in 10 seconds isnt worth much when a single melee attack hits for 250.
After you have the boots, and maybe a Sword of the Occult, its time for Kat to focus entirely on her attack damage. I suggest looking at the B.F. Sword. I suggest looking 3 times, 4 if you didn't get a sword of the occult. Yup, the next 7400 gold all go to getting copies of that one item. After you have them (I recommend keeping one item space free for a super secret surprise that you couldn't possibly see by looking at the item build at the top of the page) its time to start upgrading! Bloodthirster is the only choice. The other two choices, Black Cleaver and Infinity Edge, cost about the same, or more, but give less damage. They do give other nice bonuses, lowering defense and increase criticals, but those dont help Kat. Katarina rarely attacks with her basic physical attack, between her abilities she doesn't need to, so you almost never have a chance to get those better crits (also, no critical chance increasing gear) or lower defense...and even if you did, you do magic damage. Along the same line, items that increase damage per second without increasing attack damage provide little benefit. Attack speed, armor penetration, you simply don't use them to kill heroes.
Bloodthirsters provide one more benefit in addition to the best damage increase: life steal. This may sound a little strange since I just railed against non damage increasing melee bonuses, but I have found lifesteal an excellent way to extend an attack. After confronting another hero, even if you win, you wont be at full health. Sometimes you are all buy dead, and a trip to your base is needed to regain health. This means a long walk back to the enemies base (yeah, I'm assuming you're winning, I'm trusting like that). With the high damage you have, (4 Bloodthirsters =~500 damage) and life steal (4 bloodthirsters = 100% lifesteal) 4 hits on a minion or a creep will put you right back at full health..and ready to harass the enemy some more.
In case you were wondering, its not difficult to fully feed a Bloodthirster, at this point in the game Kat's bouncing blade will do enough damage to kill the 3 caster minions of a spawn outright, and if the fighters are left you can shunpo to one, kill it, and two physical attacks with get the others, and you are a fifth the way there. Creep spawns are about as difficult to kill.
Finally, Katarina is running around with a magical pair of boots and a bunch of knives, hitting hard, and she has accumulated some wealth, so when she goes to splurge what does she buy? A veil! Banshees Veil, a little less sweet than the traditional, a little more fitting with character, but a veil non the less. It gives health and magic resistance, and mana (oh boy!) but the main appeal is the ability to block one negative ability every 30 seconds. This continues the theme that assembled the previous 5 items, maximizing Death Lotus. The most obnoxious thing that an enemy can do is interrupt Death Lotus. This simply makes it so that they have to interrupt twice, and if they do, that's one less interrupt they can hit the rest of your team with. And if Kat got another second out of Death Lotus, that's just icing on the cake. And when not doing that, the veil can protect you from annoying abilities, like the scoundrel Gangplank's parrrley.
This does vary from the item build in the start, and tha'ts because I like how this passage flows and don't want to change it. However, after reading comments by Searz and Tinaby, I have decided that it is a better idea to get the Veil earlier. Hopefully the ability to deflect a spell every thirty seconds will increase make it harder to pin Kat down, and forgive a few mistakes she makes. If you aren't having any trouble with spells being cast on you however, or are confident in your ability to avoid them, I suggest sticking with the original description and purchasing BF swords
Other Items Used, But Not Recommended:
Frozen Mallet - 700 HP and slows on hit. Survivability isn't an issue. Not because Kat is tough, but because 700 hp doesnt make a different. When Katarina dies, she dies hard. Rarely am I shot in the back from the edge of Tristana's range. The slow does provide some value, but only in limited situations. Katarina does the most damage when confronting groups of enemies, when able to take advantage of the multiple targets Death Lotus hits, and frozen mallet will only stop one person, letting the others roam freely.
This would suggest that Rylai's Scepter would be a effective weapon, as it will slow everyone hit by Death Lotus. Unfortunately, multi-target spells only slow by 15%, and while it would also let shunpo and bouncing blade slow, you would lose 100 damage from a Bloodthirster, which I don't believe is worth it. This is a team game, someone else on your team will almost certainly be able to slow better than you, let them know you are relying on them to set you up and you have eliminated the need to carry something that slow.
Leviatian, Warmog's Armor, any item bought to toughen you up - Probally not worth it. Dont misunderstand, you will be tougher with them, and probably survive running away once or twice in a 1v1, but in team fights it wont help at all, and you will do less damage.
Mercury Treads - The reasoning is the same as why to avoid Cleanse, its the stopping of Death Lotus that is an issue, not the duration.
None of this is set in stone. These are merely suggestions, you, the player, must decide what fits what circumstance.
Katarina, Using Knives, With Style
Katarina can head to any lane to start. My preference is the center lane, I enjoy concentrating on one opponent, widdling down their life until they stay a little bit longer with not enough health and I can finish them. This requires an opponent willing to accommodate you, so don't stress out if a hero more suited for the middle lane appears, remember that it is a team game.
Harass by throwing bouncing blade as often as you can. If an opponent is in range, hit them, if they are not, hit an enemy near them, hope it reaches. Use bouncing blade to last hit units, occasionally a few at once. Shunpo can be used for that too, but be wary, shunpoing to a ranged hero, or near one, will often get you shot 2 or 3 times before you can get away, make sure it is worth it.
At level 6 you should have 4-5 kills. I kid, I kid, if you have that many kills the other team (or hero) is terrible and you can probably use the game to try out something new. But, you now have a level 1 Death Lotus. Unfortunately, at this level, with your damage, its only really useful if the enemy hero tries to kill you while you are using it. In this case you do have a good chance at killing em though. Don't think Katarina can win a 2v1 now, Kat's lotus is only targeting 1 person at once and isn't hitting hard enough tear them down and then their friend.
Float from lane to lane now, looking for low health aggressive heroes. A shunpo followed by a death lotus followed by a bouncing blade can probably do six or seven hundred damage. Stick with teamates, dont freelance unless you are sure that you handle whatever you get yourself into.
Late game, with big team fights, rely on your bouncing blade to soften up enemies. Use shunpo to get in the midst of an enemy once the real conflict starts, and start Death Lotusing. Once Death Lotus is over, either because of completion or interruption, get the hell out of there. Once you are out, turn around and consider offering another bouncing blade.
If the enemy breaks, wait until no one is being hit be Death Lotus and then charge after them, taking advantage of voracity to finish as many as you can. Don't chase the first one to leave, let Death Lotus bloom as long as it can damaging their, it deals massively more damage than Katarina's other abilities.
The Golem buff it nice to have, even though you have no mana to regen. But, because you are not fully using it, check with your team to see if there is another player who would find it more useful
One of the most important things of all? Stay alive! Keep track of who is where, traveling with a partner, dont go charging up to a turret to kill someone underneath, dont go charging up to a turret and let and enemy hero get behind Kat and kill her. Getting killed gives the enemy money (quite a bit if you are on some type of killing spree) costs you time, and stacks on your weapon that take time to replenish.
You, Arguing
AP Kat is better!
I disagree, clearly. I am going to compare this build to "Shunpo That," an AP build on this site.
Shunpo that (henceforth known as Shunpo): With soul stealer filled, she has around 690 AP. This translates to roughly 290 damage per dagger during Death Lotus, and 730 damage shunpos. Bouncing Blade stays at 232 damage.
Katarina, With Knives (henceforth known as Knives): With everything filled, she has around 530 attack. Death Lotus hits for 330 per dagger, shunpo only 220, and bouncing blade 660 damage
Not a big difference in other aspects, Shunpo has slightly higher magic penetration and better cooldowns, they have roughly the same HP, and Knives is faster (Sword of the Occult).
Shunpo that can slow enemies with abilitys, but Knives hits harder with Death Lotus, (assuming 2 daggers per second, 480 more damage over 6 seconds) and, as I have said, others almost certainly have better slowing attacks than 15%.
Shunpo can do slightly higher damage to a single target without using Death Lotus, assuming only abilities are used. With a single auto attack Knives jumps ahead in damage death (shunpo -> blade: 960 v. 880, shunpo ->attack ->blade, 1070 v. 1430) With a high damage bouncing blade, Knives is better at attacking groups of enemies, be they heroes or minions, and striking enemies that are not in her immediate vicinity.
Knives is about 1400 gold more expensive, but the much improved ability to kill minions means that you will likely end up with more more than Shunpo.
Banshee's Veil also improves team fighting ability dramatically with immunity to the first ability targeting the hero
I assumed an ideal situation because there are too many possible situations to discuss them all, and this is the one I like to think about the most.
If youre trying to duplicate my math and its not working out, make sure you are including killer instincts passive bonus, Knives' havoc, and Shunpo's Ardor.


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Here are some thoughts and images taken during my layered creative process. You can also follow me on facebook or instagram to see what I'm up to.
Alexandre Aimee
January 24th 2017,
I've been extremely hush hush about my creative process on social media. I think I'm finaly ready to share what's been going on. I've never not done art. Allot of people ask me about how long I've been doing it and I feel like I'm lying when I give a time frame.
Since I could use my little arms I've "destroyed" everything my mother loves. There is not a single book she has ever owned that I did'nt contribute some form are artistic destruction. Every surface I have ever owned or been arround has been marked by my soul.
I was not a fan of art classes in middle school. In complete honesty I had horrible grades in art cause I could'nt follow any rules. (The system is flawed but that's a conversation for another time) Its when life as I knew it crumbled I stoped making art for others and grades but for myself and to process life itself. That was 11 years ago.
From that point on I took other peoples sadness and my own as inspiration to create. My first canvas I ever did was thanks to a religious brother who was also my french teacher in 9th grade. He brought me a canvas one day.. This time of year. And pushed me to paint a narrative. I havnt stopped since.
I changed school and have been pushing myself and my craft, its not choice, well never felt like one but rather an impulse.
When I was 20 years old on the night I saw my grand mothers last breaths and watched a heat storm with my dad in the sky I decided to be selfish and take my work seriously. And I've been on an art adventure ever since.
With time my own thoughts began to fade and the thoughts and lives of others have become my main point of interest. Stories and narrative are the center of my creative process, knowing and understanding others lives, choices and perspectives. It fuels me. I have many muses and faithful family and friends who I owe endless gratitude and apologies for bringing you along the ride.
I'm about to work on an autoportrait (selfportrait) thus trying to channel my own perspective.
If you're still reading,
Thank you 😘👽


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"This is #2 of 3 gluten-free cream soups. As written, these recipes are free of Corn, Wheat, Gluten, Soy, Egg, Yeast and Nuts. NOTE FROM SOURCE: Recipe soups are those soups that one would normally find canned. If you have food allergies, the chances of finding a canned soup you can use in recipes is slim. Besides the usual allergens, you will generaly also find MSG in almost all canned soup, even many of the "health food store" type. MSG is not always listed in that way. MSG frequently hides in foods in the form of "hydrolyzed vegetable protein," "autolyzed yeast," "spices," "flavorings," and other non-specific ingredients. Here are some "semi-condensed" soups you can make from scratch and keep in the freezer for those times when you have a recipe that calls for a canned soup."
Gluten-Free "canned" Cream of Chicken Soup T-R-L
0 recipe photos
READY IN:1hr 15mins
YIELD:2 1/2 cups
Ingredients Nutrition
- 2 chicken thighs
- 2 cups water
- 1⁄2 large carrot
- 1 stalk celery
- 1 tablespoon minced onion
- 1⁄2 teaspoon sea salt
- 1⁄8 teaspoon black pepper
- 1 teaspoon dried parsley
- 1 tablespoon tapioca flour
- 1 cup half-and-half
- Makes approx 2-1/2 cups of "condensed" cream of chicken soup, which will work for most recipes calling for canned soup.
- Place the chicken in the water, bring to a boil and immediately lower to simmer.
- Add the carrot, onion and celery.
- Simmer for 1 hour.
- Carefully remove the vegetables and chicken from the broth.
- Place the vegetables in a food processor, remove the meat (not fat) from the bones, and place the meat in the food processor as well.
- Process into a puree, then return to the sauce pan with any remaining broth.
- Bring to a boil.
- Mix the tapioca flour with the half and half and stir into the contents of the sauce pan.
- When thickened, remove from heat and allow to cool.
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Thank you to everyone who came out to celebrate the opening of Forage by Something's Hiding In Here! It was such a pleasure to have Shauna and Stephen with us here all week. They continue to amaze us with all the hard work and creativity they put into everything they do. The ties are beyond beautiful, and everyone had a great time learning to tie them, thanks to our friend Barrett from Taylor Stitch. Speaking of friends, Jill from Studio Choo dropped off a stunning bouquet they made to celebrate the opening.
For our Bay Area friends, please come by the Shoppe and check out the ties soon. Seeing them in person in the gallery space is definitely worth a trip. For all of our friends across the country interested in purchasing a tie, they will be on sale at our online Shoppe beginning at 9am PST on Tuesday, July 27th.


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There are bunkrooms that have bunk beds with mattresses. You will need bedding and pillows. Lots of blankets/layers in winter!
Please feel free to
bring equipment/activities that you would like to engage in while at camp
i.e sports equipment, art activities, board games, books, resources,
Please bring at least 2 rolls of toilet paper, and 1 rubbish bag. We are
required to take all our rubbish away with us.
You will also need eating utensils, plates, cups and tea towels.
Other things that have been needed/suggested: tin opener, oven mitts, dishwashing liquid, torch/lamp, ear plugs, heaters (April-September), extension cord, multi-plug box.
On departure the camp site requires cleaning by those that attend. This
is done on the Sunday prior to departure with everyone pitching in.


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Madeira, "Ilha Jardim" (Garden Island) or "Pearl of the Atlantic",one of the most beautiful destinations in Europe. I fell in love with this small island the first time I visited. It was just me and my husband then; I was totally blown away with its scenery – simply beautiful! This time round, we visited the family with our 2 kids.
My husband's parents grew up in this island, but they moved to South Africa for better opportunities when they were young. So, in this visit they flew to Madeira to meet us and spend some quality time with the rest of the family. It was indeed an unforgettable trip. We're glad the kids met their Portuguese family.
Madeira is a small island which offers a lot of activities to all ages – from the busy streets of Funchal to the peak of Pico do Areeiro – you will be delighted with the stunning views and fun activities. There are a lot of tours to choose from; well… we are lucky to have locals as our guide. I think we pretty much did most of the Must-Do activities during our last 2 visits.
Here are my favorite activities with the kids: Read More


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|Property type
|Minimum Stay
|3 nights
The Americana Log Cabin sits nestled in the woods on a five acre lot on the North Lubec Peninsula. The cabin shares private access to a secluded beach with two other log cabins, each set apart in their own wooded nooks for privacy. This nearly new cabin has all the modern conveniences— well equipped kitchen with dishwasher, washer and dryer, 1 full bath, and 2 bedrooms. Satellite TV and local phone service means you can stay in touch with the outside world (but only if you want to!). A gas log stove keeps the cabin cozy on chilly nights. Ceiling fans keep it comfortable on warm days. Lawn furniture and gas grill provide outdoor living area. All bed and bath linen provided. Sorry, no smoking indoors. Well behaved children and small dogs welcome with prior approval, additional damage deposit (refundable at end of stay), and additional rental fee.
This HomeAway partner has demonstrated and is committed to fast response times, the best rates, and a great guest experience.
The cabin was even more beautiful than the pictures. We had such a wonderful week. We brought our tandem kayak and were able to drop it in the water right from the beach and kayak all day, catching mackerel. Tony was a wonderful host and we had everything we needed and more. We will definitely return!
We loved our stay and our friendly hosts Tony and Dottie. This was our second summer coming for the Bay of Fundy marathon. The cabin is cozy and peaceful and the kitchen well supplied for cooking simple meals. Everything is always neat and tidy with fresh sheets and towels. The ocean is just a short stroll from the cabin. The water is cold but the view is breathtaking! Many thanks and see you next year for the Bay of Fundy 2019 Marathon!
The Americana Log Cabin was ideal for us to enjoy this amazing and beautiful corner of our country. It provided us a central location from which to enjoy the numerous local sights, A comfortable cabin to "chill" and read on the porch, walk along the beach or prepare delicious locally purchased seafood in a very accommodating kitchen. Tony and Dottie are wonderful hosts and take pride in their property and community. Unfortunately, we could stay for only 4 nights and had to leave many sights, hikes, shops and restaurants waiting for our return.
I messed up with my first rental and was able to stay here. While it was not directly on the bay, it was very private; offered everything we needed to do laundry, cook, and was close to the activities we had planned. So glad we could stay here.
Great little cabin in the woods. Very clean and comfy.
We had an abosolutely great weekend getaway at the Americana cabin. It's cute and comfortable, offering just about everything you can think of that you might need. We loved the short walk to the beach and enjoyed visiting it daily during our stay- and even enjoyed lobsters on the beach the last night! Beautiful place- lovely area to explore and relax in. Would highly recommend it!
Top Thing to do in City:
* Visit West Quoddy Head Light House.
* Go on a Whale Watch.
* Eat Lobster.
* Bird watch.
* Hike our many coastal trails.
Top Thing to do in Budget:
* Picnic at West Quoddy Head State Park.
* Explore the Lubec Sand Bar Nature Preserve.
* Watch the seals "surf" through the Lubec Channel.
* Explore our varied beaches & shorelines.
* Relax and rejuvenate!.
Top Thing to do in Family:
* Hike the Boot Cove and Hamilton Cove trails.
* Eat lobster, scallops, and clams.
* Beachcomb for sea treasures.
* Take a ferry over the Old Sow whirlpool.
* Sit on the deck and gaze at our incredible stars.
Lubec is home to the West Quoddy Head Lighthouse, at the easternmost point of land in the contiguous U.S. The Down East area showcases nature at her finest. Whales, eagles, osprey, moose, and bear inhabit the region. During migration season, whales pass close enough to be seen from shore! Local whale and puffin tours provide the opportunity to observe nature up close and personal. Beach roses, wild iris, and beach peas line the pristine shoreline. The area is host to three state parks, two international parks, a national wildlife refuge, and several nature preserves, all of which offer a variety of well maintained hiking trails. The area is rich in history—the first naval battle of the Revolutionary War was fought in nearby Machiasport. Maine's own "Cannery Row" lined the waterfronts of Lubec and Eastport with sardine canning factories. You'll find it all here—rich history, spectacular scenery, abundant wildlife, and friendly people. You won't find pollution, crowds, or traffic jams.
|Refundable damage deposit
Weekly Rate: $800 - $850
May, June, September, October: $700plus 7% tax.
July & August: $800 wk plus 7% tax.
Pet Considered: One small to mid-sized dog is allowed with prior approval. Pet fee of $50/wk is added to rent.
Damage Deposit Requried: $300 refundable damage deposit. ($350 with a pet.)
Reservations and payments: Half of rent is due when placing reservation. Remaining half of rent, tax, plus refundable damage deposit due six weeks in advance of stay. Damage deposit is returned promptly after your stay, assuming there is no damage.
Personal checks accepted


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Workshop: Assistive technologies and robotics
21 March 2018Time: 3:00 - 7:00pm
Venue: QMUL campus: Room TBC
Book now
A workshop by ARQ (Advanced Robotics @Queen Mary)
The workshop will include talks on assistive technologies and their applications from invited and QMUL speakers. The talks will be followed by a reception.
This workshop is a part of the QMUL Cybathlon 2020 initiative supported by the QMUL Westfield funds and ARQ.
All interested academic and support staff, researchers and students are invited.
Free registration is required (please, follow the link). Places are limited.