text:
In this tutorial you will learn how easy it is to add a reflection beneath your drawing/text. I will be using the latest version of Gimp(2.8) for this tutorial.
To begin, let's make a new file. When creating it, open advanced options and make the background transparent.
From there, go ahead and select your text tool. Let's create some text and we'll use the word: "Reflection"
Now we need to do two things:
1. In your layers panel, right click on your text-layer and click merge down.
2. In your layers panel, right click on your layer and select duplicate layer.
(Make sure to merge first and then duplicate!)
Make sure you have the copy layer selected and then right click in the image area, then go to layer > transform > flip vertically(click this)
Line up the vertically flipped text right below the normal text as seen in the picture below(use the move tool).
Now on our copied layer, the one that is flipped upside down, right click it and go to layer > mask > add layer mask and select black(full transparency).
You can no longer see the layer. Here is where the reflection part happens! Select your gradient tool and in the tool options below select your gradient type and make it foreground to transparency.
Make sure your foreground color is WHITE! From there, go back over to your image. Click and hold the mouse somewhere in the middle of the image and then drag down holding the control key. Drag down over top of where the invisible layer is and release the click. You will now notice that there is a reflection effect! You may have to play around with this final step to get the desired results you want.
Here is the final result:
And here is an example using this technique with a background color and applying some effects such as a gaussian blur:
This technique will work on any image not just text. Feel free to play around with different filters! I find adding a blur is a nice touch.
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Creating text reflections [GIMP]
Posted 19 December 2012 - 07:06 AM
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text:
Short-sleeve Montage Print Cotton Shirt
€590.00 €590.00
All orders will be fulfilled by Burberry Limited and shipped from the UK. When you place your order, you accept Burberry's Terms and Conditions and confirm that you have read our Privacy Policy.
Size Guide
Use the Size Guide to choose the best fit for your measurements.
In this window, you can find out the shipping and returns options for the selected item.
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- A short-sleeved shirt in smooth cotton with a half-zip placket and knitted tipped polo collar, updated with runway motifs including an excerpt from William Shakespeare's 'Richard II'.
- 100% cotton
- Trim: 100% calf leather
- Short sleeves
- Point collar
- Half-zip closure
- Chest patch pocket
- Machine wash
- Imported
Item 45584721
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subset: commoncrawl


text:
Welcome to Tahosa High Adventure Base Fly Fishing Merit Badge programs for 2019. "Hooked on Tahosa" is located at the Denver Area Council's Tahosa High Adventure Base, a camp area at the foot of the Indian Peaks Wilderness Area. Approximately 20 miles northwest of Boulder, Colorado, the camp's 300 acres of woodland sits at 9,000 feet in elevation. This year, we are offering two one day-only programs on Saturday, May 4th and May 11th. For those that are interested in camping overnight, please contact the camping department to reserve your facilities.
Come experience time in intensive fly fishing instruction for Scouts, parents and leaders. Learn to cast, set up your rod and reel, and understand the nature of fly fishing. Tahosa High Adventure Base is one of the best places to learn how to fly fish. Identify different types of wet and dry flies, learn about the bugs and creatures that fish eat and how you can effectively mimic their movements. All equipment is provided to learn to fish: rods, reels, leader, tippet, and flies. Learn to tie your own flies, as well as, appreciate the importance of regulations, angler ethics and how to integrate the Outdoor Code into your fishing experience. Tahosa High Adventure Base's Tumblesom Lake is a private lake, so state fishing licenses are not required to fish.
Individuals, as well as Scouting units are encouraged to attend. Please note the participation level is capped at twelve (12) per session, in order to keep a lower student/instructor ratio and personalized instruction. Groups interested in camping overnight should contact the Denver Area Council and make their own arrangements for accommodations. Groups and individuals will be responsible for their own meals.
Due to the nature of the fly fishing sport and amount of information presented, attending Scouts must be First Class Rank or above and 13 years old by January 1, 2019. At least 50% of the group must be youth members, however adults are encouraged to participate as space permits. All youth must be accompanied by a responsible adult. No drop-offs will be accepted.
Each participant must have completed a registration and health history form 30 days prior to the start of the course.
Please Note: Until further notice there is a "Catch and Release" policy at Tahosa High Adventure Base's Tumblesom Lake.


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Simulating the Effect of Sample Size on the Sampling Distribution of the Mean
This activity has been undergone anonymous peer review.
This activity was anonymously reviewed by educators with appropriate statistics background according to the CAUSE review criteria for its pedagogic collection.
This page first made public: May 17, 2007
This material is replicated on a number of sites as part of the SERC Pedagogic Service Project
This activity allows students to explore the relationship between sample size and the variability of the sampling distribution of the mean. Students use a Java applet to specify the shape of the "parent" distribution and two sample sizes. The simulation then samples from the parent distribution to approximate the sampling distributions for the two sample sizes. Students can see both sampling distributions at the same time making them easy to compare. The activity also allows students to determine the probability of extreme sample means for the different sample sizes so that they can discover that small sample sizes are much more likely than large samples to produce extreme values.Keywords: sampling distribution, sample size, simulation
Learning Goals
Context for Use
This activity works well when each student has access to a computer, but can also be used when students share a computer and/or there is one computer with a screen projected for the whole class to see. This activity can be used with high school and college students. If done carefully, it can be adapted for middle school children.
Time involved:
5 minutes to introduce the activity
15 minutes to work, either individually or in groups
15 minutes for discussion and application of the concepts.
Description and Teaching Materials
A set of questions is available for students to attempt to answer before they interact with the simulation. No feedback is given the first time students answer the questions other than an overall score. They are then asked to use the simulation to help them discover the answers to the questions. The second time through the questions feedback and explanations are given.
The simulation is available at http://onlinestatbook.com/chapter7/SampDist_v2.html. It can be downloaded for local from http://onlinestatbook.com.
Teaching Notes and Tips
The simulation provides general instructions and step-by-step instructions. It is preferable for the students to use only the general instructions. However, if they get stuck they should refer to the step-by-step instructions.
More advanced students may wish to contrast the effect of sample size on the sampling distribution of the range with the sampling distribution of the mean.
Following the work on the simulation it can be valuable to discuss the general principle that extreme outcomes are more likely with small sample sizes than with large sample sizes. For example, students can discuss whether it is more likely that a sample of two people or a sample of five people would have a mean height over six feet.
For a period of one year, two hospitals, the larger one having about 45 births per day and the smaller about 15 births per day, recorded the days on which more than 60% of the babies born were boys (given a gender ratio of 50:50). Participants were asked which hospital recorded more such days.
Since transfer of learning can be difficult, don't expect particularly good performance on this question. You may wish to introduce this problem (without discussion) before introducing the activity.
How is the variability of the sampling distribution of the mean affected by sample size?
How does sample size affect the probability that a sample mean will be extremely far from the population mean?
References and Resources
It can be downloaded for local use from http://onlinestatbook.com.


text:
7 Options for Monitoring Home Energy Use
If you are looking for a way to stay within your budget and conserve energy at the same time, several companies now provide a wide range of tools that will help you to monitor, calculate, and effectively manage the energy you consume. Some require a fairly significant initial investment, but over time, you should be able to make up for the cost with the amount you save on your monthly utility bill.
Not surprisingly, all of these tools do not work the same way. Certain gadgets will allow you to control your air conditioning, and possibly other electronics, through your computer or mobile phone. These manufacturers are aware that utility companies' rates are higher in the afternoon than they are night, and they are developing advanced software and devices that can effectively manage your energy use and the cost that comes with it. Some tools can supply periodic energy data and even predict your energy consumption in different circumstances.
Here are some products that might suit your needs:
● The home energy-management system from American Grid is a kit with a dashboard for displaying energy data, a gateway to the Web, and related devices that will control your major appliances' use of energy and the temperature in every room. Consumers can use text messaging for controlling the power of those appliances, and the system sends data about their energy use and the related cost to a server that American Grid manages, (A subscription to the service is also required.) The company estimates that a household now paying more than $250 for their monthly energy expenses could potentially realize a savings of 20% on their bill.
● The Energy Detective (TED) from Energy Inc. shows both electricity pricing and the owner's energy consumption on the Internet throughout the day. Various packages are available to suit the individual consumer's needs, and TED can monitor energy use from the consumer's electrical panels, and the energy produced by solar panels, where that applies.
Some of the company's higher-priced products are compatible with PowerMeter, a web-based tool from Google that will use the data supplied by your TED devices to analyze your use of energy, assist you in estimating your yearly electric bill, and help you to establish some cost-savings goals. Aside from that, Energy Inc. can also provide its own software and a wireless display device for monitoring your use of energy.
● Current Cost markets simple gadgets that you can attach to your meter for displaying basic data related to your general energy use. One package includes the PowerMeter mentioned above, and this will provide more detailed information regarding your energy use and ways for setting your goals for saving energy.
● With the eMonitor from Powerhouse Dynamics, customers can monitor their use of energy at what is called the "circuit level." They can zero in on energy-use patterns throughout the home and adjust the power used in the heating and cooling systems, along with certain appliances. They can also receive alerts when their use of energy exceeds the norms and calculate their "carbon footprint" as well.
● DreamWatts, which is sold by PowerMand, is a setup that is similar to what American Grid has to offer. The customer purchases an Internet gateway, which collects information wirelessly from thermostats and similar devices to measures the energy electrical circuits consume. To receive the data and the analysis, they must also subscribe to the company's service.
● For consumers who only require remote control of their thermostat and lighting, Alarm.com offers some less expensive solutions. The company sells its security software and sensors through retailers, who can provide an energy-monitoring system at little or no cost. This enables consumers to program their air conditioning, lights, and heating system to operate at various times, and it can be done via their Smartphone or computer.
● The PowerCostMonitor from Blue Line Innovations is energy-monitoring equipment that customers attach to their meter for real-time information regarding their energy consumption. The device, which transmits the data wirelessly to a display that is as large as digital clock, is available at several retail outlets.


text: Miranda Kerr and hubby Orlando Bloom continue to dispel recent rumors their relationship is on the rocks. After they were seen smooching at Vanity Fair's Oscar party, top model Kerr tells us they tore up the dance floor at Madonna's Oscar fete. But, "Madonna's got moves I could never do," Kerr said at an event for the Victoria's Secret Fabulous collection yesterday in Manhattan. "[Orlando] is a great dancer," she added. "We love to dance if we go out to an event, or we dance at home." The beauty said the couple's son Flynn, 2, isn't too shabby either. "When he was in my stomach, I used to play a lot of classical music," Kerr said. "He really loves 'The Nutcracker.' He also likes Justin Bieber."


text: SARATOGA SPRINGS -- The lives of Saratoga's Latino community will come to life through numerous photos taken by local Latino photographers in the sixth annual "Vision -- A look at life behind the scenes." The photo project will be presented by the Saratoga County Economic Opportunity Council's Latino Community Advocacy Program during a reception from 5:30 to 8 p.m. Aug. 6 on the Clubhouse porch at Saratoga Race Course. Marcella Ducasses, program coordinator for the Latino Community Advocacy Program, said the program was established to expose members of the Latino community to art programs they normally don't have access to. In turn, the rest of the community can take a look into how they live. "A lot the time, their activities go unnoticed, so the other purpose is to give the community a glimpse into their lives and their positive contributions to Saratoga," Ducasses said. "It's a way to engage the community and let them know they are here." The program has eight to 12 participants per year, and the participants engage in four hands-on workshops with professional photographers. The participants also are given their own digital cameras, which they can keep for the duration of the project. Economic Opportunity Council Executive Director Julie Hoxie said when the project first started, participants used to use black-and-white, disposable film cameras. However, a photographer urged them to go digital. The project begins at the beginning of June, when many Latino workers begin working at Saratoga Race Course. Ducasses said not only do the participants bring in track photos but many different kinds of photography as well, including landscape and family portraiture. "Some of it is of mundane experiences, which is what we want to show," she said. At the end of the project, the participants submit their best work to be shown at the reception, where a group of jurors selects the winners. The exhibit is then shown at various venues throughout the Capital Region. Ducasses said the program has been successful and has received notoriety over the years. Rather than just handing out information about the Latino Community Advocacy Program, Ducasses said the photo project provides a more appealing way to convey Latino community lifestyles. "It's a nice vehicle to get the community aware of what is going on," she said. Hoxie agreed. "It gives them a voice that's visually representing their life and experiences here," she said. All of the proceeds from the reception will then be put back into the Latino Community Advocacy Program, where the money will help set up a wide range of services designed to aid local Latino community members, including financial, legal, educational, medical and childcare services. "The whole premise of our program is to help the immigrant population overcome the language, social and cultural barriers," Ducasses said. The reception will feature music by the Garland Nelson Ensemble and drinks and hors d'oeuvres will be served. Some of the photographs also will be featured in a free exhibit from 5 to 8:30 p.m. Tuesday at the Tang Museum and Art Gallery at Skidmore College.


text:
Yes it is 2016 grounded Reggae, Dub and Bassmusic are important and vital then ever!
Selectah EL P from Leipzig let you hear it in a very fine 45 minits mix for that Vibes Promotion session. Including some (yet) unreleased and forthcoming tunes.
Dub/Reggae/Bassmusic is one element to show a face against the inhumanity in this times!
A playlist you find here!
Listen Vibes Promotion live every Friday 8 pm here.
Also check our big Vibes Promotion Showarchiv of the last 10 years.
Podcast: Download


text:
The pop icon takes in two spinning classes during Grammy Awards weekend
Who's that girl? In your L.A. spinning class?
Yep, that was Madonna, who got her Grammy weekend cardio in at Cycle House in West Hollywood.
The famously fit singer and her daughter Lourdes Leon, 17, showed up together for a spin class on Friday. The pop icon, 55, loved the workout so much that she returned Saturday for a second class.
Arriving in black top, black beanie and sunglasses – and also what one source said was a grill – Madonna donned black riding gloves for the 45-minute full-body ride. For the arms section of the class, the buff mom of four chose 2 lb. weights to complete her workout.
She later cooled down with her trainer Nicole Winhoffer inside the spinning studio as the rest of her class stretched outside in a courtyard.
Madonna is set to perform on the Grammy Awards show Sunday night, making her first appearance since 2006 when the seven-time Grammy winner shared the stage with Gorillaz.


text:
The Devil Theory Of War - An Inquiry Into the Nature of History and the Possibility of Keeping Out of War
Charles A. Beard
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Title: The Devil Theory Of War - An Inquiry Into ...
Publisher: The Vanguard Press
Publication Date: 1936
Binding: Hard Cover
Book Condition: Very Good
Dust Jacket Condition: Good
Edition: First Edition
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text:
Australia 21-6 South Africa, Tri-Nations, September 5
Wallabies halt Springbok juggernaut
September 5, 2009
Australia's Adam Ashley-Cooper celebrates scoring the Wallabies' first try
© Getty Images
Peter De Villiers Al Baxter Robbie Deans Jean de Villiers Fourie du Preez Jaque Fourie Will Genia Bryan Habana
South Africa's march to the Tri-Nations title was halted by a committed Australia side in Brisbane, the Wallabies running out 21-6 victors on a day that could have seen the Springboks claim the southern hemisphere crown with a win.
South Africa's Suncorp Stadium hoodoo continued as their tournament took a turn for the worse on the same ground at which they were humbled 49-0 by Australia in 2006. Adam Ashley-Cooper and James O'Connor scored second-half tries for the home side, who were reinvigorated by ascendancy at the scrum and the spiky presence of Reds scrum-half Will Genia.
Matt Giteau had earlier given the Wallabies the lead with two penalties and a cheeky drop-goal as the Springboks struggled to exert any dominance. Victory for the hosts also released the pressure valve on coach Robbie Deans, who has suffered at the hands of the press after a disappointing run of defeats.
Peter de Villiers' Springboks will now require a result against the All Blacks in Hamilton next weekend, the tournament in the balance with two games remaining and New Zealand nine points behind the leaders.
Genia settled his nerves early with a well-judged box kick but it was the Springboks who enjoyed the first meaningful possession, sending Jean de Villiers crashing up the middle before spreading the ball wide quickly.
De Villiers' second involvement was to send his centre partner Jaque Fourie scything through the Wallabies' line, eerily reminiscent for the home side after the first-phase tries conceded in Perth last weekend. The Wallabies regained the ball, Giteau showing confidence and attacking ambition in sparking a counter attack from his own try-line. Ashley-Cooper collected the pass out wide and broke to halfway, where an offside Bakkies Botha conceded a penalty to gift Giteau the first points of the game.
The Springboks worked good possession with a rolling maul and began to exert their dominance at the lineout, but it was the Wallabies who controlled the tempo and worked a dynamic turnover thanks to prop Benn Robinson after Fourie du Preez attempted to spark a counter attack.
A searing break from Fourie, Habana and fullback Ruan Pienaar was halted by another Wallabies turnover, the Springboks looking to play expansively when possession was available but not seeing the rewards produced by their pressure-based game plan in earlier games.
The Wallabies went close to scoring the opening try when Lachie Turner broke away on his wing following excellent work from Genia. The Waratahs wing looked sured to score but was hammered in touch by a breathtaking last-ditch tackle from Habana. Rather than dwell on their missed chance, the hosts were immediately back on the front foot when Giteau punched in to a gap, ignoring a lurking Berrick Barnes, before being dropped by another Habana tackle.
Stung in to action, the Springboks worked the phases on the Australian 22. Juan Smith powered through his work but the pressure was released by a superb low hit from Tatafu Polota-Nau on Heinrich Brussow. They found more joy from the set piece when De Villiers again waltzed through the midfield defence following clean lineout ball, his pass finding Fourie on a path to the line only for Drew Mitchell and O'Connor to produce a pair of try-saving hits on the man winning his 50th Test cap.
Despite their added venom in attack the Springboks fell further behind to Giteau's second penalty, secured after a ruck infringement by Brussow. Morne Steyn clipped a drop-goal over following another dismal Australian lineout loss but the home side began to dominate at the scrum - where Robinson tore in to Springbok skipper John Smit and rightly won a penalty.
Giteau extended the lead when he salvaged a crumbling attack with a well-judged drop-goal, three points that were handed back almost immediately when Steyn curled in his first penalty of the evening before the break.
The second-half was heralded by a crunching tackle from Smit on replacement Australian hooker Stephen Moore, sparking an attack from Pienaar and Ndungane. Again the Boks lost the ball in contact and could have paid with five points had Giteau not dropped the ball with a six on three overlap outside him.
Handling errors began to creep in to the Australian attack, yielding possession to the Springboks who retreated quickly back to a pressurised kicking game.
Rocky Elsom arched past several Springbok defenders following a free-kick at the lineout, their frantic cover hauling him down and conceding a penalty. Genia caught the visitors napping and darted for the line, but his opposite number du Preez was equal to the task and ripped the ball clear.
From the ensuing scrum the Springboks soaked up waves of pressure, driving the Wallabies backwards with every tackle. Ndungane forced a knock-on from Elsom out wide and the danger was cleared with a scrum penalty against Robinson.
Two majestic kicks from Steyn and du Preez worked the Springboks in to good territory, but first their scrum and then, more surprisingly, their lineout coughed up possession. Giteau seized upon some stagnant ball with a clever grubber for Peter Hynes to chase, Adi Jacobs covering out wide for the Springboks. The centre eluded Hynes' tackle and threw a suicidal pass to de Villiers which was picked off by Giteau. The fly-half dived in at the corner but was just forced out by a textbook tackle from Fourie.
With their home fans reaching boiling point, the Wallabies finally had the try that their attacking endeavour deserved. Barnes, who had struggled previously, took charge and crabbed across field before unleashing Ashley-Cooper, who cut back against the grain and raced through the gap to score.
The Brumbies centre raced to celebrate with the jubilant fans and once calm had been restored Giteau missed a regulation penalty secured following another shaky Springbok scrum.
O'Connor sealed the victory with a late opportunist score, following up his own kick and pouncing as pressure was on the Springbok defence. Schalk Burger and Bismarck du Plessis conspired to cough up the ball and the teenager was on hand to spark the celebrations.
© Scrum.com
Communication error please reload the page.


text:
Thanks to MindLoveMisery's Menagerie for hosting this week's Tale Weaver's prompt about a quest, such as the ones JRR Tolkien writes about in his famous books.
Dragons are here, I know it
Dragons beware, my sword is sharp
Sharp as the knives hid on my body
Sharp as the tongue of my wife
Wife said, "Do not go"
Wife begged, yet I went
Went through the haunted forest dark
Went through the storms, muck, and mire
Mire as quicksand, sucked in my body
Mire that almost swallowed my life
Life burnt a flaming hole so wide
Life's flame would not flicker out
Out of the muck and mire pulled
Out of certain death to rescue a princess
Locked in a tower for my Lord, my King
Locked in a tower and languishing.
Languished she did for centuries
Languished as a spell had been cast
Cast, so she would always sleep
Cast, because evil always hates
Hates beauty and goodness
Hates who this princess is said to be
Be afraid though, I warn you, friend
Be vigilant in your task to save
Saving the princess isn't the challenge
Saving her, I wondered, where is the dragon?
Dragon she rose from the depths of beauty
Dragon was the the princess herself
Herself screaming, "I am the dragon"
Herself shouting, "I will eat you whole"
Wholly she transformed in that fiery beast
Wholly she was a scaled, sulphereous demon
Demon who cried, "I am no damsal in distress"
Demon who seethed, "I protect me"
Me, I gazed upon the languishing beauty
Me, my eyes met the dragons yellow-eyed stare
Stared into my soul, saw I was a ruin
Stared into my heart, saw I was wretched
Wretched cursed princess, the dragon?
Wretched as the princess waiting
Waiting and no one came so she grew tired
Waiting as she wrecks her vengeance
Vengeance because no hero is true
Vengeance, she can depend only on herself, no heroes
Hereo, the archetypal kind who abuse poor maidens
Heroe, is there such a man who ever existed?
Existed a hero she once did love
Existed her hero but he never came — she remains cursed
Cursed though she be, I could not destroy the beast
Cursed, she knows not why she is punished, cursed.
Beast but still a girl, so I left, ashamed I could not save her.
Cursed, she lingers on my mind, the maiden, the dragon as one
©Mandibelle16. (2017) All Rights Reserved.


text:
A 20-year-old New Jersey man is charged in the murder of a recent high school graduate in an altercation sparked by an apparent case of mistaken identity, authorities said.
The dispute began when Jonathan Lafontaine of Totowa, with three friends inside a Jeep, pulled up alongside a Mercedes on Union Boulevard in Little Falls, prosecutors said. The Mercedes was filled with a group of five young people, including 18-year-old Brian Topoleski, returning from a night out at a local restaurant.
The people inside the Jeep thought they recognized someone in the Mercedes and began to yell out to get their attention, according to prosecutors. They quickly realized they were mistaken but the exchange set off an altercation between the two groups.
The Jeep took off toward a house in Little Falls, and the Mercedes followed. Once the Jeep arrived at its destination on Jacobus Avenue -- the home of a friend of Lafontaine -- the groups again exchanged words, prosecutors said. Lafontaine walked out of the Jeep and went inside his friend's home.
Meanwhile, the group inside the Mercedes, including Topoleski, walked out of the car and went toward the Jeep where the rest of the occupants -- a boy and a girl -- were still inside, according to prosecutors.
At that point, Lafontaine reemerged from his friend's home with two steak knives and approached Topoleski and his friends, who by that point were preparing to leave. That's when Lafontaine punched Topoleski and stabbed him in the chest, prosecutors said.
Lafontaine retreated into his friend's home after the stabbing, then hid in the backyard, authorities said. SWAT teams were called to the house, and eventually took him into custody without incident.
Topoleski was declared dead at St. Joseph's Regional Trauma Center.
Lafontaine is charged with first-degree murder and two counts of criminal possession of a weapon. Attorney information wasn't immediately available.
Prosecutors said the friend inside the Jacobus Avenue home had tried to stop Lafontaine as he left the home with the knives, and that the friend sustained cuts to his hand in his attempt.
Topoleski had just graduated Cedar Grove High School and was set to attend Montclair State University this fall, his family said.
"My brother was just a nice, kind, caring kid," said Topoleski's brother Robert. "Anytime my sister had to go to swim practice, he would be the first to take her. My dad needed help at work, he would be there. Anytime his friends needed anything, he would drop anything for them."
-- Brian Thompson contributed to this report.


text:
While they were inhaling burgers, I got a phone call from the ER, and wandered off to a quieter area to talk. Without paying attention, I ended up standing next to a kiosk selling phone accessories.
Dr. Grumpy: "Is she on Coumadin?"
Kiosk Guy: (comes over, taps me on shoulder) "Hey! You need a new iPhone case!"
Dr. Grumpy: "No thank you, I'm busy right now. What did her head CT show?"
Kiosk Guy: "This one is on sale! It lights up when you're on the phone!"
Dr. Grumpy: (waves guy away again) "Who's her cardiologist? Do they know what's going on yet?"
Kiosk Guy: "Your phone case is falling apart! You should get this one!"
Dr. Grumpy: "Hang on, ERP... Look, this is an important call. Can't you see I'm on the phone?"
Kiosk Guy: "No! That's why you need a case that lights up!"


text:
Paula Gable will lead the meditation. The exact format may vary daily based on the needs and requests of those in attendance.We anticipate that the program will be comprised primarily of 20 to 40 minute silent meditation periods, and perhaps some chanting, guided meditation, walking meditation, reading and discussion of meditation-related topics.The details are up to you.
Back to All Events
Earlier Event: December 2Eclectics Jam
Later Event: December 3Drum Circle


text:
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Small Town Heritage with a World Renowned Reputation.
Spring City has been manufacturing the finest cast iron and aluminum cast lamp poles for more than 150 years. All poles are manufactured in the small historic Pennsylvanian town of Spring City in a captive foundry. All poles are optimized for cast iron, but most are available in ductile iron and heavy wall aluminum as well. From the simple to the complex, Spring City cast lamp poles have endless possibilities, which are not limited to the below standard product offering.
Spring City's ability to produce precise historical reproductions and stunning custom pieces is unmatched in the market. Contact us today more information and pricing.


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September 18, 2012, 4:50 pm
Day's Best: Yampa Valley sandhill crane festival kicks off
The very first Yampa Valley Crane Festival began Sunday with visitors lining up to view the iconic species in action.
The festival includes activities through Wednesday designed to teach people about the birds and the challenges in conserving their habitat.
The event is a perfect opportunity to head for the mountains to learn about the crane's place in Colorado.


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How to Make a Kate or Plying Ball
To make two or three-ply yarn, you need the singles that you have spun, either on two bobbins if you are using a wheel, or rolled into a plying ball if you are using a hand spindle. If your singles are on bobbins, you should use a kate to keep them organized. Many beautiful kates are available, but you can easily make one yourself. If you are going to ply from a ball, you should use a basket or box—even your shoe can work in a pinch—to keep it from rolling away.
Use a Kate
If you are using a wheel, you need to transfer your singles to a kate before you can ply. You can make the kate that is pictured here using materials that are easily available. You need a sturdy cardboard box or a small basket, four metal knitting needles, and a handful of cloth-covered hair elastics. Make sure that the knitting needles are the right size to fit through your bobbins.
Push the knitting needles through the box or the holes in the basket. Try to line the holes up so that the bobbins are straight.
Twist the hair elastics over the knitting needles to prevent them from sliding out.
Slide the bobbins onto the knitting needles.
Secure with the hair elastics.
Make sure that the threads are all coming off the bobbins in the same direction, either over the top or from underneath. If they do not pull off in the same direction, they will make a terrible tangle.
Make a plying ball
If you are using a hand spindle instead of a wheel, you need to make a plying ball to wind the yarn off the spindle. If you do not have a nøstepinde, you can use a paper towel core or dowelling.
Take the ends of the two singles that you are going to ply, and tape them together to the top of the nøstepinde, paper towel core, or dowel.
Hold the core in one hand and the joined singles in the other. Wrap the singles on a diagonal around the core so that they lie side by side.
When you have wrapped the singles so that half of the core is covered, give the core a quarter turn and continue wrapping.
Keep turning a quarter turn and wrapping until you come to the end of your singles.
If the two balls of singles don't come out evenly, continue to wrap the remaining single around the plying ball in the same pattern.
Tuck the ends of the singles securely in place on the outside of the plying ball.
Unfasten the singles that are taped to the core. Tie a big, obvious knot in them.
Keeping the knot in one hand, slip the ball off of the core.
You now have a plying ball that you can pull easily from the knotted end in the center of the ball.


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We are your College Station Team Building Specialists
Not in College Station? Team building activities near you
Job Title: Practicum / Intern / Co-op
Location: Multiple locations throughout North America
Contract: Full Time, Temporary
Here at College Station Team Building, we make it our mission to prove that helping others can be both fulfilling and fun. If you're looking for a new, rewarding challenge, join our highly motivated team and find out how great it feels to be an integral part of an energetic company. We have worked hard to earn our reputation as experts in the team building industry and we invite you to make our team even better!
We love what we do. We offer a variety of exciting and challenging team building activities and employee training programs for corporate groups of all sizes. Our solutions include TV themed activities such as Minute To Win It, The Amazing Chase, CI: Crime Investigators, and many more.
Here are just a few reasons why our loyal customers continue to come to us for all of their team building event needs:
We work with Fortune 500 companies such as Toshiba, Microsoft, Disney, Coca-Cola and Google to provide team-building activities for groups of all sizes. Our customers choose to work with us because they know we are committed to meeting their company needs with the perfect team building activity.
We're proud to support many Canadian and international college / university recreation, tourism and business programs by providing students hungry for experience, a variety of practicum opportunities in our exciting industry. We accept applications for the following positions:
Marketing - Experience what it is like to plan, coordinate and execute marketing communications that go out to thousands of top fortune 500 companies across Canada and the USA. Be part of a dynamic, fast paced marketing team in North Vancouver, and gain hands on experience working across a variety of marketing disciplines such as e-marketing, online marketing, print, advertising, public relations and marketing analytics.
Events - If you're looking for a career in events management, then come experience what it's like to be part of our Events Division in either North Vancouver, Calgary or Toronto. The team are responsible for planning and executing thousands of events anywhere in size from 50 to 1,000 plus. Develop your communications, leadership and public speaking skills, and gain valuable experience in events management.
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Generalist - If you're looking to gain a broad range of experience, then you may be interested in our Generalist Program in North Vancouver. Every year we take on students looking to gain a broad range of experience across multiple business units as part of their practicum, internship or coop placement. If you're adaptable and willing to move around departments, then this is the opportunity for you, as you'll get to spend time in our Sales, Marketing and Events Division.
If you are a motivated individual who wants to make work less like work for people around the world we want to hear from you. We're looking to grow our driven, passionate and talented team with people like you. Contact us through our online form or give us a call at 1-877-713-7238.


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State Wants to Ban Homeschooling
Take Action
Please sign the international petition if you live outside of the Netherlands.
Please sign the Dutch petition if you reside within the Netherlands.
Read More
Examiner.com: "Petition seeks to stop ban on homeschooling in the Netherlands"
Authorities are making plans to ban home education in the Netherlands. Despite a review last year by the Dutch parliament that affirmed homeschooling and its supervision, Mr. Sander Dekker, secretary of state for education in the Netherlands, announced to the Dutch House of Representatives in July that he plans to make it impossible to homeschool.
The Netherlands Association for Home Education, Nederlandse Vereniging voor Thuisonderwijs (NVvTO), has issued a petition to help stop the announced ban on homeschooling that has already gathered more than 2,000 signatures.
Cry for Help
Peter van Zuidam, NVvTO secretary, is pleading for the help of the international homeschool community.
"The NVvTO is doing everything in its power to prevent this amendment to the law," said van Zuidam. "We ask everyone who supports home education and the rights of parents to add your name to our petition, so we can make very clear to the members of parliament that the world is watching them."
Homeschooling is currently legal in the Netherlands by an exemption to the compulsory attendance law. The petition calls on the Dutch parliament to maintain the current right to exemption from compulsory schooling or to make home education itself a legal right within the Compulsory Education Act.
Freedom under Fire
Michael Donnelly, director of international relations at HSLDA, echoed Van Zuidam's call for help.
"Dekker's plan to ban homeschooling completely in the Netherlands comes from the same place as German officials and even American academics who believe that the state should control all aspects of a child's education," Donnelly said.
"This totalitarian thinking is at odds with the liberty expected in free society. As homeschooling grows, clashes between statists and free people are increasing. We can help fellow homeschoolers and strengthen our freedom by standing against totalitarians like Mr. Dekker. We showed this in the Wunderlich case in Germany, and I ask homeschoolers everywhere to send a message to Dutch officials by signing this petition."
Protect Your Family
If you or someone you know is not a member of HSLDA, will you consider taking a moment today to join or recommend us? Your support enables us to defend individual families threatened by government officials and protect homeschooling freedom for all. Join now >>


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The RV industry in northern indiana is beginning to breath a sigh of relief, however hesitantly, according to The Goshen (Ind.) News.
Shipments began a turnaround in January, after dropping from a high of 31,400 units in April 2008 to only 5,600 units in December. That turnaround has not gone unnoticed. Shipments totaled 13,300 in April.
"We're going through a period right now of relatively good order backlog, which is a significant improvement over what it was three months ago," said Sid Johnson, director of marketing at Jayco Inc., Middlebury, Ind. "In fact, in the last 60 days, in terms of order intake, we're at a good 50% of what we were doing last year at this time."
A recent release from the company noted positive dealer attitudes at their "Homecoming" event held last week. And although the company's RVs are constructed by a 1,200-employee work force, approximately 55% of its size a year ago, new products will be coming out. Johnson pointed to a new product line that the company will introduce near the beginning of the new model year.
"This year is going to continue to be challenging, but I think everyone, at least everyone at Jayco, is enthusiastic about our prospects for next year starting this fall and winter," he said. "We're spending a lot of time on product development and aggressive promotion programs throughout North America."
He added, though, that it's too early to be counting any chickens.
"There's no doubt recovery has begun in the industry and the economy as a whole," Johnson said, "but the bottom line is we're not out of the woods yet."
At Keystone RV Co., in Goshen, demeanors are also bright.
"We definitely saw things pick up about six weeks ago, and we're seeing it from retail orders on our dealers' lots," Bob Martin, Keystone's executive vice president and chief operating officer, said.
Martin said everything was shut down this week to give their employees a well-deserved break. Next week, four additional plants will be opening back up.
"We definitely got busy, and it happened quickly," he said.
So far this year, about 150 employees have been rehired due to increased production rates. Where their dealers were having problems selling units early in the year, they are now beginning to sell out.
"It's been a good feeling and we've been blessed this summer," Martin said. "Earlier this spring, we didn't know what to expect, so this is a very welcome event for our company and our community."
He pointed out that company officials are still watching out for the rest of the year, as nobody in the industry can accurately predict what could happen when the weather turns colder and the prime RVing season passes.
"We really don't know what to expect going into the fall," Martin said. "Much of what hindered us last year were banks and retail and wholesale financing, and that's the X-factor we don't know."
Local RV dealerships are feeling the turnaround as well.
In Shipshewana, Wana RV & Engine Center President Gary Martin is excited.
"Our numbers for June are much, much better than what they were last year, and we're actually starting to catch back up," Miller said.
Like the manufacturers, he has noticed business picking up recently in particular.
"It's just been the last four weeks that we have really been hopping that we can't keep up, which is a good thing," he said. "Fifth-wheel, travel trailers both are good. It doesn't matter if they're lightweight or bigger units. Sales are up right now."
Ewing's Outpost RV Sales Manager Dennis Johnson has seen much the same at their dealership.
"As of today here, we've had more traffic than we've had for a while," he said. "Long story short, we've done actually pretty well."
According to Dennis Johnson, they haven't had all the problems some dealerships have seen, but times have still been tough. He said their business has shifted but stayed relatively constant since the beginning of the year.
"People's sights have dropped down some as far as dollar amount that they want to spend and the size of the unit they want to purchase. Larger, higher-priced units are not selling as well," Johnson said. "Over the last couple of weeks that's changed some, we're starting back in again with the bigger units."
They are now up to about one sale each day, or between 30 and 35 units each month.
"We can't complain," Dennis Johnson said. "We're still here, and that's one thing a lot of people can't say."
He spoke confidently when asked about the future of the industry.
"I think, from everything I hear, there's going to be a little lull here for a while yet," Johnson said. "But I think it will come back and come back a little bit stronger than before because of pent-up demand."


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Historian remains key ally of right
Sens. Ted Cruz and Rand Paul were headline attractions at a conference in Iowa this summer that brought hundreds of conservative Christian pastors together to talk faith and politics.
But they weren't the only draw.
Story Continued Below
The trim man with silver hair who drew in close to Cruz in a prayer huddle also had considerable star power: the evangelical historian David Barton.
An informal adviser to several prominent Republican politicians, including Mike Huckabee, Newt Gingrich and Michele Bachmann, Barton argues that the United States was founded as a Christian nation and must return to those roots.
His ideas have shaped the social studies curriculum in his home state of Texas. Now, he's gunning for even bigger influence, advising state legislators across the country on how to fight the Common Core academic standards that the Obama administration is promoting. And that's not all; Barton hints he'll soon be back in the arena of presidential politics, advising candidates looking to appeal to the religious right.
"I remain available to whoever wants to move that ball down the court," Barton told POLITICO.
Just last summer, that kind of role seemed unlikely. Barton's reputation was in tatters. It appeared doubtful that an ambitious politician would stand next to him, much less turn to him for advice. But Barton has rebounded so completely that his appearance in the inner circle of the Cruz prayer huddle in Des Moines was deemed a smart move — for Cruz — by political analysts.
Barton has huge standing among "social conservatives that make up a significant base of a caucus electorate," said Craig Robinson, editor of The Iowa Republican website. "You want to appeal to those people if you're a Ted Cruz or a Rand Paul."
Barton's abrupt, and short-lived, fall from grace began with the publication in April 2012 of his book "The Jefferson Lies," which portrays Thomas Jefferson as an orthodox Christian who saw no need to separate church and state.
Secular critics had long denounced Barton as a fraud who manipulates and misrepresents history to serve political goals. With the publication of "The Jefferson Lies," several dozen academics at Christian colleges stepped forward to join the chorus.
Led by Warren Throckmorton, a professor of psychology at Grove City College, the Christian scholars tore apart the new book, pointing out a bevy of errors and distortion. Several pastors picked up the thread, organizing a boycott of Barton's publisher, the Christian publishing house Thomas Nelson. The critiques gained so much steam that Barton's book was voted "the least credible history book in print" in an online poll by the History News Network.
Barton rejected the barrage of criticism as mean-spirited, politically motivated and just plain wrong. But in August, his publisher withdrew "The Jefferson Lies." A senior executive explained to NPR that Thomas Nelson couldn't stand by the book because "basic truths just were not there."
It was a stunning repudiation of Barton's credibility.
But to his critics' astonishment, Barton has bounced back. He has retained his popular following and his political appeal — in large part, analysts say, because he brings an air of sober-minded scholarship to the culture wars, framing the modern-day agenda of the religious right as a return to the Founding Fathers' vision for America.
"It has been shocking how much resistance there is to critically examining what Barton says," said Scott Culpepper, an associate professor of history at Dordt College who has critiqued Barton's scholarship. "I really underestimated the power of the political element in evangelicalism."
In March, Barton gave his presentation on America's biblical heritage to dozens of state legislators in Kansas. In May, he spoke at the official National Day of Prayer breakfast at the Fort Leonard Wood Army base in Missouri. He rallied activists at the National Right to Life Convention in June with a rousing speech drawing on the Declaration of Independence to make the case for abortion restrictions. Cruz followed Barton in the program and echoed his analysis to thunderous applause.
"I'm not in a position to opine on academic disputes between historians, but I can tell you that David Barton is a good man, a courageous leader and a friend," Cruz told POLITICO. "David's historical research has helped millions rediscover the founding principles of our nation and the incredible sacrifices that men and women of faith made to bequeath to us the freest and most prosperous nation in the world."
This fall, Barton will share that message before audiences in Oklahoma, Nebraska and Kansas. He also continues to travel to Washington to lead his signature Capitol tours — sponsored and often attended by members of Congress — at which he expounds on America's Christian roots.
Radio host Glenn Beck's publishing company, Mercury Ink, has even announced plans to republish "The Jefferson Lies," although a spokesman would give no details about timing, print run or whether the manuscript would be edited to address the criticism.
Barton's enduring popularity "embarrasses the academic community," Throckmorton said. But, he added, no matter how loud the scholarly chorus, Barton has a trump card: His message "is useful politically."
Indeed, political strategists say Republican candidates are wise to consult Barton and hitch their wagon to his star.
Barton's endorsement was widely seen as a big boost for Cruz's upstart campaign to represent Texas in the U.S. Senate. He also campaigned for Florida Sen. Marco Rubio in 2010 and has long backed Texas Gov. Rick Perry.
"When it comes to evangelical outreach, there's nobody more effective than David Barton," said Jamie Johnson, a member of the Republican Party's state central committee in Iowa. Johnson led Pennsylvania Sen. Rick Santorum to his upset victory in the 2012 Iowa caucus and is backing Cruz for 2016, though he does not officially work for the Texas senator.
Barton, who has a bachelor's in Christian education from Oral Roberts University, addresses a range of topics in his speeches and in a daily radio program produced by his organization, WallBuilders, which is based in Aledo, Texas. He has dismissed global warming as implausible because of a 15-year plateau in the average temperatures on Earth. He has suggested that the federal government should regulate homosexuality as an unhealthy lifestyle, akin to eating too much trans fat.


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Call for Papers
Note: The Call for Papers is now CLOSED. Notices of acceptance have been sent. Participants should expect to recieve information via email regarding payment of registration fees. Thank you to everyone who submitted a proposal!
Conference Dates: October 17-19, 2013
Keynote Speaker: Kriss Ravetto
Submission Deadline: June 28, 2013
This conference will seek to challenge the borders imposed on Cinema: between senses, ideologies, cultures, even the fluctuation of the medium itself in terms of its technologies, production, distribution, and exhibition. From the stage to the gallery to the arcade, expanding to television and digital platforms, the space of Cinema is no longer confined to that of the movie theater. By broadening our definition to include multiple viewing environments and experiences as they have existed throughout time, we can engage new spaces and new ideas.
We as Cinema scholars strive to uncover fertile ground in which to excavate lost thoughts while cultivating new ones. We seek to explore the embrasures of cinematic evolution, the gaps containing affect, creation, and possibility – the "space between stations" after the rupture and before the order. With the stretching of the screen, what we have called the Cinema is changing; our discourse must adapt accordingly.
Possible paper topics and panel discussions may include but are not limited to the following:
-interdisciplinary or cross disciplinary approaches to cinema
-new media, multimedia, digital media -cross media, cross genre
-experimental cinema -cinema online, new directions, and interactivity
-new modes of viewing, new viewing environments
-television, VOD platforms
-gender studies and sexuality
-guerrilla cinema/ wall and street projections
-the future of film studies
-the space of the screen and beyond it: voice over, neo-benshi, etc.
-evolving technologies/digital theory
-art and cinema
-intercultural cinema
-migrant and diasporic cinema
-ethnicity and national identity
-transnational or postnational cinema
The CSGSA of San Francisco State University welcomes proposals, presentations, and panel suggestions from graduate students and faculty. Submissions should include a 250 word abstract and proposal of visual presentation. Presentation visual aids are strongly encouraged.
Submission Deadline: JUNE 28, 2013
Please send submissions to: firstname.lastname@example.com


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NRTA and NIRS: Protecting Teacher's Retirement Security
NRTA: AARP's Educator Community has joined the National Institute on Retirement Security (NIRS), a research and education not-for-profit organization in Washington, DC. NIRS was established to fill a gap in understanding on the value that defined benefit pensions play in ensuring retirement security for workers, as well as their value to employers, and the economy.
Through the collaboration with NRTA, active and retired educators can now access NIRS products and services — at no cost. NIRS programs and information serve as valuable tools to help educate local officials and stakeholders about teacher retirement issues. NIRS member services include: Research, Education Materials, Counsel, Commentary, Events/Speakers, and Technical Assistance.
NRTA and NIRS will continue to work together to help ensure that public pensions remain the cornerstone of retirement security for America's teachers.
About National Institute on Retirement Security
Founded in 2007 by the National Council on Teacher Retirement, the Council of Institutional Investors, and National Association of State Retirement Administrators, NIRS has a diverse membership of organizations including employee benefit plans, state or local agencies that manage retirement plans, trade associations, financial services firms, and other retirement service providers.
Join the Discussion0 | Add Yours
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NISE Network product
Goodman Research Group conducted this large sample treatment/control group Outcomes Evaluation on The Amazing Nano Brothers Juggling Show, a live theater performance that uses story-telling and juggling to model and dramatize the nanoscale world: atoms, molecules, nanoscale forces, and scanning probe microscopy. The performance was developed by the Strategic Projects Group at the Museum of Science and is offered in live performances and is also part of the Talking Nano 6-DVD set. The evaluation separately analyzed three groups: children ages 6-12, adults 18 years and older, and middle school field trip teacher leaders.
Product category


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Mini Poppy Painting 2
Mini Poppy Painting 2
from 10.00
hese abstract mini bouquets are studies for the series Joy & Love .
These pieces are just like flowers, here to make you feel better & happier, some sunshine for the soul.
- watercolour on paper, signed and float mounted onto mountboard
- ARTWORK FLOAT MOUNT: measures 105x148 mm/4-1/8 x 5-7/8 in
- Fits perfectly into A6 frame or choose mounted and framed A4 option
- FRAMED : With mount measures 240 x330mm/ 9.4 x12.9"
- recorded, signed for delivery,
sold out


text: Information about products and services CONTACT : For non-European residents / End-Customers from Kazakhstan, Russia and Ukraine: Please check our global network to select your territory and country of residence for the contact details and website link of the Mitsubishi Motors-representative assigned to your market. CONTACT : For European residents: - If you have questions related to your Mitsubishi-vehicle, our products and/or services, please contact your local Mitsubishi dealer first. In order to locate the closest dealer near you, go to the countries section and visit the website of the national Mitsubishi Motors representative. - In case your dealer and the national Mitsubishi Motors-representative have not been able to handle your request or it is needed that Mitsubishi Motors Europe B.V. is to be contacted, please find below our available contact opportunities:


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News & Notes: Game 134
Saturday, August 27, 2011 (7:00 p.m.) – Fifth Third Field
Radio: WWKB AM 1520, bisons.com
Buffalo Bisons (57-76, 5th(-18.5), North)
RHP Brian Sweeney (4-5, 4.50)
Toledo Mud Hens (65-70, 4th(-17.0), West)
LHP Andrew Oliver (8-10, 4.73)
TODAY'S GAME: Tonight, the Buffalo Bisons face the Toledo Mud Hens in the finale of a four-game series from Fifth Third Field (7:00 p.m.). The Herd is 3-4 on its current roadtrip, having won the first three games in Columbus before dropping four straight. The Bisons return home to Buffalo tomorrow to host Rochester for two games and Syracuse for two games.
VS. THE HENS: The Bisons are still in search of a solution to Fifth Third Field. Buffalo has lost the first three games of this series and has now lost 12 straight in Toledo. The last time the Bisons won at Fifth Third Field was a 5-3 victory on May 28, 2008. A win today would snap that streak and give the Herd a 4-4 split in their 2011 season series against the Mud Hens.
BACK ON THE BUMP: RHP BRIAN SWEENEY will start tonight as RHP Miguel Batista was promoted to New York yesterday. Sweeney went 2-3 in five starts with the Herd after being signed on May 6. He was then moved to the bullpen where he has worked in relief in 23 of his last 24 outings. Sweeney did make a spot start in the second game of a doubleheader on August 15 against Durham. He allowed two runs in 3.1 innings of a no-decision.
MIXED RESULTS: The Bisons scored 31 runs in the first three games of this roadtrip, racking up victories of 15-3, 10-2 and 6-4 over the Clippers. However, the Herd has produced just six runs in four losses since. Buffalo was shutout 5-0 by the Clippers on August 23 and 1-0 by Toledo last night.
SPIN CONTROL: INF JORDANY VALDESPIN brings a five-game hit streak into tonight's game. The Triple-A rookie has averaged .381 (8-21) with four doubles, three RBI and two runs scored Since August 22. In the seven games this roadtrip began, Valdespin has hit .353 with a home run and seven RBI.
BISONS BITS: OF JESUS FELICIANO has 366 hits for his three-year Bisons career, 12 shy of Bill Selby's modern era record total of 378…INF VALENTINO PASCUCCI has 90RBI on the season, tops in the International League. He also ranks second with 69 walks on the year…RHP DALE THAYER is sixth in the IL with his 18 saves.
METS UPDATE: The Mets (63-68) shutout the Braves, 6-0, thanks to a dominating performance from LHP CHRIS CAPUANO. The southpaw allowed just two hits and struck out 13 batters to improve to 10-11 on the year. The Mets now have the weekend off, as both games scheduled have been postponed because of Hurricane Irene.


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Some of the images on this site show men in various stages of undress. This is intended to be educational and open a dialogue about men's bodies; gay, bi, trans, and queer men's body image; mental health; and health and wellness.
By continuing, you're confirming that you're at least 18 years old and agree to this site's terms of access.


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Contracts (Spanish-Language)
CONTRACTS: (Basic Rules)
- If a salesperson or other business representative primarily negotiates with a Spanish-speaking consumer in Spanish, the consumer must be given a written Spanish-language translation of the contract, even if the consumer does not request it.
- This rule applies to the following contracts: CC 1632
- Credit sales contracts for goods and services of all kinds, including automobiles
- Almost all loans or other extensions of credit for personal, family, or household purposes, except loans secured by real estate
- Loans secured by real estate, if arranged by a real estate loan broker or made by a personal finance company
- Contracts for renting, leasing, or subleasing apartments or other kinds of housing (including mobile homes), for longer than one month (Month-to-month and week-to-week rental contracts do not apply.)
- Contracts for the payment of legal fees for services from lawyers
The rule does not apply to the following contracts: CC 1632
- Home improvement contracts
- Contracts involving a seller not engaged in a trade or business
- Subsequent documents authorized or contemplated by the original Spanish-language document
- Contracts in which the Spanish-speaking consumer has negotiated through his or her own interpreter
However, the last exception applies only if the consumer's interpreter:
- is able to speak fluently and read with full understanding both English and Spanish,
- is 18 years or older, and
- is not employed by or made available by the salesperson or business.
If the contract is for a loan from a bank, credit union, savings association, or other "supervised financial organization," the law does not require the entire contract to be translated. Only the following information must be translated into Spanish and provided to the consumer.
- The amount financed
- The annual percentage rate
- The amount and due dates of the payments
- Other credit disclosures required by the Truth in Lending Act
- The Spanish-language version of the contract must be given to the Spanish-speaking consumer before the contract is executed. It is unlawful to require the consumer to sign a purchase order or any other legally binding document before he or she receives the Spanish-language translation of that document.
- At the same time and place where a consumer and a business negotiate in Spanish and enter into a contract, a Spanish-language notice of the consumer's rights must also be displayed. The notice must be displayed in a conspicuous place. It must state that a businessperson who negotiates in Spanish must provide a Spanish version of any contract given to the consumer.
- If a business does not provide a Spanish-language contract when the law requires it, the consumer can cancel the contract. The consumer can cancel the contract even it it's been assigned to a financial institution. In this case, the consumer should ask the business with which he or she originally made the contract to return any money the consumer paid. If the consumer received any merchandise, it must be returned to the original business.
Before Signing a Contract: CC 1632
- Read the contract carefully. Make sure you understand all of it. If you don't like what you see, don't sign.
- If you don't understand the contract, take it home overnight. Don't rush into signing anything, especially if the salesperson insists that it be signed right away. Remember: You'll have to live with what you sign.
- Discuss any important or complex contract with a close friend or attorney. Be sure that other businesses aren't offering a better deal.
If you don't like what the contract says, ask the business to cross out words you don't like and add words that you believe should be there. Don't be afraid to add terms if the matter is important to you. A merchant who really wants to close the deal may agree to your changes.
However, you should realize that many merchants will not change their contracts under any circumstances. Also, if the contract is preprinted on a form, it is usually offered to you on a "take it or leave it" basis. You are usually not permitted to change the contract. In those situations, you probably shouldn't enter into the contract.
- If the salesperson has made oral promises that are not written in the contract, insist that they be written into the contract. It's okay if the inserted changes are handwritten. Don't do business with a company that won't put oral promises into writing.
How to Cancel a Contract: CC 1688 et seq.
- Promptly notify the other party of your decision to cancel. Return or offer to return whatever you have received from the other party, on the condition that the other party returns what he or she received from you.
- Give your cancellation notice in writing to the other party, and also give a copy to anyone else who is involved in the transaction. The cancellation notice might also describe other ways that you can cancel the contract.
- If you purchased something from the other party, he or she usually must return your money within 10 days after receiving your cancellation notice. However, the contract may state another time period for the return of your money, which you'll usually have to abide by unless it's unreasonable.
Where to Go for Help:
- Your local consumer affairs agency.
- If you have a question about whether a contract you have signed or are thinking about signing is legally binding, it's best to talk with an attorney. Contact your local legal aid society or a private attorney.
Guide to Abbreviation:
CC = Civil Code
Directory of State Agencies Directory of Federal Agencies Return to DCA Home Page


text:
person(redirected from -person)
Also found in: Dictionary, Thesaurus, Medical, Financial, Encyclopedia.
In general usage, a human being; by statute, however, the term can include firms, labor organizations, partnerships, associations, corporations, legal representatives, trustees, trustees in Bankruptcy, or receivers.
A corporation is a "person" for purposes of the constitutional guarantees of equal protection of laws and Due Process of Law.
Foreign governments otherwise eligible to sue in United States courts are "persons" entitled to institute a suit for treble damages for alleged antitrust violations under the Clayton Act (15 U.S.C.A. § 12 et seq.).
Illegitimate children are "persons" within the meaning of the Equal Protection Clause of the Fourteenth Amendment to the U.S. Constitution.
The phrase interested person refers to heirs, devisees, children, spouses, creditors, beneficiaries, and any others having a property right in, or a claim against, a trust estate or the estate of a decedent, ward, or protected person. It also refers to personal representatives and to fiduciaries.
n. 1) a human being. 2) a corporation treated as having the rights and obligations of a person. Counties and cities can be treated as a person in the same manner as a corporation. However, corporations, counties and cities cannot have the emotions of humans such as malice, and therefore are not liable for punitive damages. (See: party, corporation)
personnoun autonomous being, being, caput, chap, character, fellow, homo, human, human being, human creaaure, individual, living being, living soul, member of the human race, mortal, mortal body, mortalis, party, somebody, someone, soul
Associated concepts: adult person, artificial person, compeeent person, credible person, disorderly person, fictitious person, injured person, natural person, person aggrieved, person in need of supervision, poor person, third person, unauthorized person
See also: actor, character, individual
personthe object of legal rights. There are two kinds of legal person: human beings and artificial persons such as corporations. A PARTNERSHIP in England is not a separate legal person but in Scotland it is said to have quasi-personality.
PERSON. This word is applied to men, women and children, who are called
natural persons. In law, man and person are not exactly synonymous terms.
Any human being is a man, whether he be a member of society or not, whatever
may be the rank he holds, or whatever may be his age, sex, &c. A person is a
man considered according to the rank he holds in society, with all the
rights to which the place he holds entitles him, and the duties which it
imposes. 1 Bouv. Inst. n. 137.
2. It is also used to denote a corporation which is an artificial person. 1 Bl. Com. 123; 4 Bing. 669; C. 33 Eng. C. L R. 488; Woodes. Lect. 116; Bac. Us. 57; 1 Mod. 164.
3. But when the word "Persons" is spoken of in legislative acts, natural persons will be intended, unless something appear in the context to show that it applies to artificial persons. 1 Scam. R. 178.
4. Natural persons are divided into males, or men; and females or women. Men are capable of all kinds of engagements and functions, unless by reasons applying to particular individuals. Women cannot be appointed to any public office, nor perform any civil functions, except those which the law specially declares them capable of exercising. Civ. Code of Louis. art. 25.
5. They are also sometimes divided into free persons and slaves. Freemen are those who have preserved their natural liberty, that is to say, who have the right of doing what is not forbidden by the law. A slave is one who is in the power of a master to whom he belongs. Slaves are sometimes ranked not with persons but things. But sometimes they are considered as persons for example, a negro is in contemplation of law a person, so as to be capable of committing a riot in conjunction with white men. 1 Bay, 358. Vide Man.
6. Persons are also divided into citizens, (q.v.) and aliens, (q.v.) when viewed with regard to their political rights. When they are considered in relation to their civil rights, they are living or civilly dead; vide Civil Death; outlaws; and infamous persons.
7. Persons are divided into legitimates and bastards, when examined as to their rights by birth.
8. When viewed in their domestic relations, they are divided into parents and children; husbands and wives; guardians and wards; and masters and servants son, as it is understood in law, see 1 Toull. n. 168; 1 Bouv. Inst. n. 1890, note.


text:
Obama's home-state govs in jeopardy
The Democratic governors running President Barack Obama's adopted state and childhood home could be headed for embarrassing electoral defeats this year, despite increasingly active assistance from the White House.
Illinois Gov. Pat Quinn trails Republican businessman Bruce Rauner in recent polls, while Hawaii Gov. Neil Abercrombie is being crushed in sparse polling ahead of this Saturday's Democratic primary against a little-known state senator, David Ige. And even if Abercrombie wins on Saturday, he starts the race behind Republican Duke Aiona, thanks in part to a third candidate on the ballot.
Story Continued Below
Both incumbents could still win reelection, but experts say the fact that they're struggling — and even trailing — is, in part, a sign of Obama's waning political influence in his home and native states and proof that presidential endorsements only go so far.
A loss by Quinn, in particular, would be historic. No incumbent governor from the president's home state and party has lost reelection since 1892 — when livestock breeder Claude Matthews, an Indiana Democrat, won the governorship during the Republican presidency of Benjamin Harrison — according to Eric Ostermeier, a University of Minnesota political researcher. In 16 chances since then, the incumbent has prevailed.
To reverse the momentum, the White House has stepped up on behalf of Quinn and Abercrombie in recent weeks. Obama recorded an unusually personal radio ad for Abercrombie — a lifelong friend — describing the governor as "ohana," the Hawaiian word for "family." And at a Democratic National Committee fundraiser in Chicago last month, first lady Michelle Obama plugged Quinn, saying, "We're going to do everything in our power to make sure he gets over the finish line," according to the Chicago Sun-Times.
The president — who's still popular in both states despite lagging ratings nationally — plans to be even more actively involved in both races in the "near future," according to a source familiar with Obama's political operation.
Ige, Abercrombie's vastly outfunded primary opponent — the incumbent outspent him 7-to-1 from July 1-25 — said he's not sweating the presidential intervention because Obama's influence in Hawaii has faded.
"I'm disappointed that he chose to [get involved in a competitive primary]," he said. "But I think he gave up his right to vote in Hawaii. He's not a resident in our community and he doesn't get a vote in our primary."
( Also on POLITICO: The latest race ratings)
In a state as small as Hawaii, Ige said, face-to-face contact is more valuable than "an endorsement from someone out of state, even the president of the United States."
That puts Ige on the same page as Aiona, the likely Republican nominee in Hawaii, who led either Democrat and a third candidate, former Honolulu Mayor Mufi Hannemann, in a recent poll.
"Even though the president may still be popular with a lot of people in Hawaii … his policy issues got a lot of pushback," Aiona, a former lieutenant governor under GOP Gov. Linda Lingle, said in a phone interview. "It's not as unconditional as it was two, three, four years ago."
Likewise, in the Land of Lincoln, Rauner aides have been buoyed by recent polls showing their candidate up double digits against Quinn, although Quinn has pointed to other surveys showing a narrower contest.
( All the polls, all in one place)
Jim Edgar, a former Republican governor, said Obama can help Quinn fundraise and drive up the African-American turnout — two critical elements of Quinn's path to victory — but that his influence in the large state is limited.
"I don't think Obama's standing has as much impact on Illinois as other states just because of the nature of Illinois," he said. Noting Obama's meteoric rise from the state Senate to the U.S. Senate to the presidency, Edgar said Obama never had much time to build a political machine in his home state.


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Happy Holidays Dear Friends of Spark Club. We have been invited by Dallas Social Venture Partners (DSVP) to attend a holiday party at Reunion Tower. RSVP Today. Per DSVP's message,
Partners, Friends & Investees are invited to come celebrate the season with Dallas Social Venture Partners. We will enjoy food, fun and friends as we look back on 2010 and 10 years of social impact in the Dallas community. Wolfgang Puck will cater appetizers and we'll share a Holiday Toast at 6:30pm. Please carpool or take DART. Parking is $10 valet at the base of Reunion Tower or $6 in nearby lots.
There will also be a Socially Conscious Marketplace ala bigBang
As seen at the bigBANG! conference earlier this year, DSVP will feature a Socially Conscious Market Place for all of your holiday giving. The market place will feature products of nonprofit ventures that fund the mission AND socially minded businesses who are measuring a double/triple bottom line.
So, If you know of a business / startup that might be a good fit for this crowd, please email Sarah Jane at firstname.lastname@example.com. There are 2 slots left and we want them to go to Spark Clubbers if possible. More info here: http://bigbangtx.org/save-the-date-dec-9-holiday-party-and-alt-gift-market/


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Proof How Indie Film Requires So Much Support/
If we didn't have the Indie Film support organizations, you wouldn't have indie films in the theater. Cinereach, IFP, Film Independent, SxSW, Tribeca, Sundance, and yes, my new home, the San Francisco Film Society -- it takes more than a village; it takes a freakin' army.
The proof is in the pudding. Look at all the films in theaters this week. All these films were discovered at Sundance and supported by these various organizations. Where would they be without them? And that's just the tip of the iceberg. And just the start. If you don't go see them -- and soon -- our very culture will be threatened!
BEASTS OF THE SOUTHERN WILD written by Benh Zeitlin and Lucy Alibar and directed by Benh Zeitlin
HELLO I MUST BE GOING written by Sarah Koskoff and directed by Todd Louiso
KEEP THE LIGHTS ON written by Ira Sachs and Mauricio Zacharias and directed by Ira Sachs
LITTLE BIRDS written and directed by Elgin James
SLEEPWALK WITH ME written by Mike Birbiglia, Seth Barrish, and Joe Birbiglia and directed by Mike Birbiglia and Seth Barrish
COMPLIANCE written and directed by Craig Zobel
THE WORDS written and directed by Brian Klugman and Lee Sternthal


text:
VirusScan Enterprise for Linux (VSEL) detects and removes viruses and other potentially unwanted software on Linux-based systems. VirusScan Enterprise for Linux uses a web browser interface and a powerful McAfee scanning engine — the engine common to all our antivirus products. A few years ago, the Linux operating system was considered a secure environment, but there are now more occurrences of software specifically written to attack or exploit security weaknesses in Linux-based systems. Increasingly, these systems interact with Windows-based computers. Although viruses written to attack Windows-based systems do not directly attack Linux systems, a Linux server can harbor these viruses, ready to infect any client that connects to it..Data Sheet
During the installation of this McAfee endpoint suite, the VirusScan Enterprise for Linux client and associated management files were checked into your McAfee ePO server. A deployment task was automatically created for you as well.
The McAfee Agent for Linux
Before you can utilize VSEL, you will need to deploy the McAfee Agent for Linux to provide communication with the McAfee ePO server. On most Linux systems, the agent can be installed manually using an installation script (install.sh) that McAfee ePO created when the agent was checked into the McAfee ePO Master Repository. The agent can also be installed from McAfee ePO on Red Hat Enterprise and Ubuntu client systems. Once the agent is in place on client systems, you can run the deployment task to install the software, and schedule updates and scans.
Manual Installation of the McAfee Agent
The install script (install.sh) for the McAfee Agent for Macintosh is in the following directory on the McAfee ePO server:
McAfee Agent Deployment
The following operating systems support installing the agent from McAfee ePO:
Note: Enable SSH on the Linux systems before installing agent from McAfee ePO.
*** Comment the following line in the /etc/sudoers file on Red Hat operating systems. Default required
Deploying VirusScan Enterprise for Linux
In this section you will assign the deployment task for VirusScan for Linux.
Clients will retrieve and run this task the next time they poll the server and install VirusScan Enterprise for Linux.


text:
Used Car Dealership Business Plan
eBook (PDF), 37 Pages
Price: $6.25
This business plan is based on an actual Used Car Dealership business. It can be reformatted into any market or any other business context. It has the financials, marketing plan, and all other business plan requirements and major headings. These major headings may include those similar to the following: I. Executive Summary II. General Company Description III. Products and Services IV. Marketing Plan V. Operational IV. Management and Organization VII. Personal Financial Statements VIII. Startup Expenses and Capitalization IX. Financial Plan Research Bibliography
Available in PDF Format
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Product Details
- Copyright
- China Doll Publishing (Standard Copyright License)
- Edition
- First
- Published
- August 26, 2009
- Language
- English
- Pages
- 37
- File Format
- File Size
- Product ID
- 5355419
Formats for this Ebook
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|Any PDF Reader, Apple Preview
|Supported Devices
|Windows PC/PocketPC, Mac OS, Linux OS, Apple iPhone/iPod Touch... (See More)
|# of Devices
|Flowing Text / Pages
|Learn more about ebook formats and e-readers
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text:
Actor Matthew McConaughey only cuts his hair when it's a full moon – in order to preserve his luscious locks.
The "Magic Mike" hunk started losing his hair in the late 1990s and began Regenix on his scalp to stave off baldness.
McConaughey still uses the product to this day and he swears by its ability to keep his mane in tip-top shape, telling Elle magazine, "Back in 1999, my hair was fallin' out, so I started (using) this stuff. And, son of a gun, if I didn't bring my hair back so well that people thought that I went and got plugs. … I believe that I'm proving the product."
In addition, the 43-year-old studies the lunar calendar to determine when it's the best time to get a trim.
He says, "If you want your hair to be thicker, cut it when the moon is about to be full – a heavy, full, waxing moon. Do not cut it when the moon's waning.
"Why do you think that (farmers) plant at certain times of the year? It's not hippie-dippie stuff. Look, how do they know when you reap, when you sow, when you plant?"


text:
What REA Produced: Idea and strategy for the brand, Identity, verbal identity, website, signage, stationery, collateral
About Sabre: Sabre Real Estate Group is a full service retail real estate brokerage company with a core focus on tenant and owner representation. SABRE also offers retail, restaurant and franchise consultation services, investment sales and property management. SABRE was founded by Jayson Siano formerly of CB Richard Ellis and Kenneth Breslin of Breslin Realty Inc. to adapt to the ever changing industry and stay ahead of the curve. While the economy right sized the real estate business over the past few years Siano and Breslin decided to change with the times and build a company for the future.
How REA approached the design process: As part of the brand creation, REA developed a proposition focused on service quality. 'Beyond Real Estate' helped articulate the company's commitment to customer service and value, which became an integral aspect of the brand communications.


text:
Whole body vibration may help elderly get up and go
When the elderly can't exercise, stints on a vibrating platform may help older adults become slightly stronger, faster and more agile, according to a small short-term study.
Exercise is the best option for good health in older age, lead author Alba Gómez Cabello told Reuters Health in an email. But for those unable to perform aerobic exercise, this vibration technique "could be an easy and quick treatment to improve physical fitness."
The method involves standing on top of a flat platform about the size of a boogie board that sends mild vibrations through the feet to the rest of the body, while the person does exercises such as standing or squatting. Bending the knees helps transmit the vibrations, said Cabello, who studies growth and exercise at the University of Zaragoza, Spain.
In the new Spanish government-funded study, 24 men and women over 65 performed 10 squats held for 45 seconds on the vibrating platform, with a minute rest in between, three times per week for 11 weeks. The study also included 25 people who did not take part in the vibration exercises.
There were some differences between the groups by the end of the study, although they were small. Those who did the exercises were, on average, able to do two more reps of upper and lower body strength exercises, had almost half an inch more lower body flexibility, and walked 33 yards one second faster than before the vibration training, according to results published in the journal Maturitas.
"Whole body vibration is an easy and quick way of exercise that stimulates muscles and improves fitness," said Cabello.
Mixed results
In theory, vibrations help activate muscles, strengthen bones and improve circulation in people of all ages - similar to the idea behind vibration belts marketed as weight loss tools in the 60s.
The vibration platforms, which cost between $150 and $3000, have shown mixed results in recent research, improving balance and muscle tone in some studies but failing to prevent bone loss in postmenopausal women in another (see Reuters story: reut.rs/v23Os3).
There still isn't enough evidence to convince most exercise scientists to advocate the devices, according to Wojtek Chodzko-Zajko, who studies aging and physical activity at the University of Illinois at Urbana-Champaign.
"We don't say a single thing about whole body vibration," he told Reuters Health of a position paper on aging and physical fitness he authored for the American College of Sports Medicine in 2009. "That doesn't mean it's a scam, it means there's really been very little study of this kind of intervention."
The vibration group did squat reps, while the comparison group did not, so some of the fitness improvements could have been due to the squatting exercises, rather than the vibration.
"This showed an improvement in motor performance on simple tasks," said Chodzko-Zajko, who was not involved in the study. "That doesn't necessarily correlate with quality of life."
A better assessment of the true health of older adults would take into account whether whole body vibration influences chronic conditions like heart disease, and mental health, depression and anxiety, he said.
Another recent study found the vibration platforms could be introduced to nursing homes and would benefit residents, at least for improved balance.
Chodzko-Zajko's 82-year-old mother has a whole body vibration machine in her bedroom, which she uses every morning to "loosen up her joints."
He offers her the same advice he would offer anyone: "I don't think it's going to do you any harm, but don't stop doing your regular exercise routine."


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"Phone Calls From The Towers"
I will presume that you're all familiar with "KEVIN COSGROVE's" (alleged) gut-wrenching 911 phone call - which ends abruptly when the WTC2 that he's supposedly calling from, collapses - and we hear Kevin screaming "OOH CHRIST!" (even though the atrociously poor/low-res audio of that supposed phone call have made most people interpreting these words as "OOH GOD!"...)
KEVIN COSGROVE http://en.wikipedia.org/wiki/Kevin_Cosgrove
What you may not know, is that the dramatic Kevin Cosgrove tale gained worldwide attention through a British Channel 4 propaganda "documentary" called "9/11: Phone Calls From The Towers". (2009). As it is, there exist a host of different versions of that Kevin Cosgrove audio track - which has been liberally used/edited by official 'documentaries' and 'official conspiracy movies' alike - such as "9/11 MYSTERIES" (an umpteenth phony truther video by the mysterious "Sophia Shafquat").
"9/11 MYSTERIES" features THIS soundtrack of the last words of "Kevin Cosgrove" :
KEVIN COSGROVE's last words - according to "911 MYSTERIES";
http://www.septclues.com/VICSIMS/KevinC ... teries.mp3
CHANNEL 4, features THIS soundtrack of the last words of "Kevin Cosgrove" :
KEVIN COSGROVE's last words - according to "CHANNEL 4 TV";
http://www.septclues.com/VICSIMS/KevinC ... annel4.mp3
Here's my audiowave analysis of the two versions:
Oh ok - so Channel 4 edited out that "OOH CHRIST", so as not to hurt the sensibilities of their viewers. Yeah, rrright!


text:
Ever since I did a story in late June on Ed McCaffrey's two-day camp for youngsters with Down syndrome, I've been looking forward to writing a sequel.
At the time, Valor Christian coach Brent Vieselmeyer told me Colorado State was recruiting his fullback, George Talanoa and the whole world was offering scholarships to quarterback Brock Berglund.
It wasn't just a welcome recruiting tip. All of Valor's players deserved commendation for their one-on-one mentoring with the campers. I noticed that day that even if the campers at times looked overwhelmed, the Valor players thoroughly enjoyed themselves.
I had a chance to chat with Talanoa Monday. "It was a great experience, we were having fun with them," Talanoa said of the camp. "Looking at the smiles on their faces, it was great for them to come to the camp."
Something tells me Talanoa might be listed as a two-star but he brings some five-star credentials to Fort Collins.


text:
Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
Moderators: honeev, Leonid, amiradm, BioTeam
Post Reply
11 posts • Page 1 of 1
- Garter
- Posts: 1
- Joined: Wed May 04, 2011 6:06 pm
can anyone tell me what are the problems associated with longer cloned seqence?How will you go upon investigating your hypothesis?
this is an assignment qestion and i couldnt find the answer
this is an assignment qestion and i couldnt find the answer
Hola, Today to clone big genes the way is amplify them with PCR from the whole DNA of the organism which carry it. First we design two oligonucleotides wich hybridize with the 5´and 3´ends and carry restriction endonucleases sites to introduce the gene in a subcloning plasmid and after production purification and digestion introduce the gene in an expression plasmid to produce the codified protein.Two are the more frequent problems with long genes, one wich is difficult to find unique sites of cloning wich don´t broke your gene at the subcloning time(As more long more probability to find cut sequences) and second as much longer is the gene more probability of mutations introduced by PCR enzyme. The first problem is overpassed studiying the sequence and preparing the subcloning with rare enzymes, because the commun ones will cut the gene. and the second is solved sequencing the PCR amplified sequence, compare with the original (there are infotmatic tools to do it)and see if the mutations produce changes in the frame (horrible you can´t follow this way) or the mutatios are silent, they only change one neutral aminoacid and the change dont have any risk(gly x val), but if the change means the change of a neutral aminoacid by a charged one (glyx pro) it will affect to the protein conformation. Probably there are more aspects but for me these are more relevants, buena suerte
Hello, I would like to insert an enhancer element into a cis-reporter plasmid (luciferase) to test promoter activity of my gene of insert. I found the consensus enhancer element sequence for my gene, consisting of only about 9 nucleotides. However, when I look at pre-made constructs, they recommend multiple insertions e.g. (TGACTAA)7
How should I know how many times I should insert my enhancer, and should there be gaps in between or pure palindromic?
Tx in advance!!
How should I know how many times I should insert my enhancer, and should there be gaps in between or pure palindromic?
Tx in advance!!
Re: cloning
I am attempting to clone a gene which i have amplified thru PCR. I have added HindIII and NotI sites at its 5' and 3' ends. I have ligated this gene into a vector after double digesting both of them by same HindIII and NotI enzymes. After transforming it into DH5 alpha cells and isolating plasmid i am double digesting it with same enzymes but not getting the insert released out. I have tried sequential digestion and simultaneous digestions. But no success. However when I am doing PCR of gene using this plasmid as template then I am getting gene amplified which i can see in agarose gel. To add more to my problem whenever I am sending this plasmid for sequencing then all my reactions are getting failed! PCR amplification confirms presence of my gene but restriction digestion is not yielding me the concerned gene. Can there be a possibility of defective enzyme?? If anybody knows anything then please help me out.
- Garter
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- Joined: Tue Jul 19, 2011 9:43 pm
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text:
03/06/2016 09:46
Maize prices closed higher by 0.88 per cent on Thursday at the National Commodity & Derivatives Exchange Limited (NCDEX) as a result of a rise in the demand from exporters and poultry industries. At the NCDEX, maize futures for June 2016 contract closed at Rs. 1,375 per quintal, up by 0.88 per cent, after opening at Rs. 1,374 against the previous closing price of Rs. 1,363. It touched the intra-day high of Rs. 1,376.
USA, China and Brazil are the top three maize producing countries in the world while the prominent exporters of maize are USA, Argentina and Brazil. Chief importers are Japan, EU, Malaysia, Taiwan, Indonesia etc.