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Interface - Comunicação, Saúde, Educação
versión impresa ISSN 1414-3283
FERREIRA, Janise Braga Barros et al. The regulatory complex for healthcare from the perspective of its operational players. Interface (Botucatu) [online]. 2010, vol.5Selected edition, pp. 0-0. ISSN 1414-3283.
This investigation aimed to evaluate aspects of the outcome from implementing the Regulatory Complex (RC) for public healthcare system organization in Ribeirão Preto, Brazil. The functional domain of the RC formed the scenario. Interviews were conducted with workers in different categories, within the administrative and operational levels of the RC. The material was analyzed using thematic analysis. The findings showed that the RC caused changes to the organizational accessibility and equity of the healthcare network, for both outpatient and hospital care. The need to create a resolutive and humanized network was highlighted. The RC was shown to be a useful evaluation and management tool. Its implementation changed the subjects' work processes and had little recognition among SUS users (Brazilian Unified Health System).The evaluation showed that, despite the short time since implementation, the RC strategy has the strength to collaborate towards SUS sustainability, although investment, dissemination and improvement are needed.
Palabras clave : Healthcare regulation and supervision; Health equity; Healthcare service accessibility; Comprehensive healthcare; Health assessment.


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PCL notes: Federowicz starts fresh
'Topes catcher makes positive impression in Triple-A debut
By Chris Jackson / Special to MLB.com | August 22, 2011 6:58 AM
Los Angeles Dodgers fans were almost uniform in their dislike of the three-team trade that sent outfield prospect Trayvon Robinson to Seattle and brought Federowicz and two pitchers to the Dodgers organization.
The stated intent by Dodgers general manager Ned Colletti was to acquire a catching prospect, something Los Angeles lacked in its farm system. Federowicz said he understands the frustration of Dodgers fans.
"Yeah, they gave up Trayvon -- he's a great player and now he's in the big leagues, doing his thing up there," Federowicz said. "That's tough to lose.
"I guess there is a little bit of pressure to show fans what I've got. But I think it'll eventually work out the way the Dodgers want it to."
So far with the Albuquerque Isotopes, Federowicz has shown the defensive skills that put him on the prospect map while also turning it on offensively. He's hit .327 (17-for-52) with four home runs and 11 RBIs in his first 15 Triple-A games.
"They like to pitch backwards up here," Federowicz said. "You don't see quite the fastballs you would at Double-A. I think their command is a little better up here. That's going to be the biggest adjustment for me. I'm used to seeing fastballs, and I don't see them anymore."
Dodgers director of player development DeJon Watson had the opportunity to see Federowicz play in Albuquerque during the Isotopes' last homestand Aug. 3-10.
"It's a pretty small sample size," Watson said. "You're still trying to get a good feel for the player. He's doing some good things. He's generating good bat speed. His defense has been good. I think there's still some things for the coaches on this team to work on with him."
Fellow catcher A.J. Ellis has been impressed with Federowicz's work ethic.
"From the very first inning he caught, you could tell he's an outstanding defensive catcher," Ellis said. "He can flat-out catch. It's pretty impressive to see a guy come in and put forth the effort he has to learn pitchers on this team and not change who he is."
Watson noticed that as well.
"Fed has shown he's willing to put in the work," Watson said. "He's usually one of the first guys here, he's one of the last guys to leave. He seems to be trying to grasp as much information as he can to help the pitchers have the best chance to be successful. It's been good to see."
Federowicz said he just wants to soak in everything he can to prepare for the Majors.
"You can definitely feel the difference in levels as far as competition and just the overall aura around the clubhouse," he said. "There's a lot of big league guys here. That's good -- you get to feed off them, see how they act, see how you're supposed to act."
Web gem and then some: Nashville turned a triple play against Omaha on Saturday night in a most unlikely fashion. With runners on first and second, Clint Robinson hit a flyball to center field that Logan Schafer jumped to catch. The ball bounced off the tip of his glove, then off his head before finally landed in his glove. Both runners strayed too far from their bases and were forced out to end the third inning as the Sounds went on to win, 4-1.
Back in action: Former National League Rookie of the Year Chris Coghlan played in his first game since June 16 on Saturday night for New Orleans. Activated from the disabled list and assigned by the Marlins to Triple-A on Friday, Coghlan went 2-for-5 with two doubles, an RBI and a run scored. It was not enough, however, as first-place Round Rock rallied from a seven-run deficit to win, 10-9, and lower its magic number to five.
Showdown series: Another former Rookie of the Year, Angel Berroa, propelled first-place Reno to an 8-7 win over second-place Tacoma on Saturday. The Aces extended their lead in the Pacific North Division to 6 1/2 games thanks to Berroa's two-run double in the eighth inning, which erased a one-run deficit. The two teams continue their series through Tuesday.
Chris Jackson is a contributor to MLB.com. This story was not subject to the approval of the National Association of Professional Baseball Leagues or its clubs.View More

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Say it isn't so.
Trader Joe's, which is coming to Denver soon, is raising the price on one of its staples. The wine known as Two Buck Chuck (Charles Shaw shiraz or cabernet sauvignon) is going from $1.99 a bottle to $2.49 in California.
The wine has been more expensive in other parts of the country for a while. For instance, it has been $3 a bottle on the east coast. Yet, the $2 bottle was a curiosity — something to try.
There's no word yet on the pricing for the wine in the Denver store, which will be the only Colorado Trader Joe's to sell booze, but we suspect it would be difficult to hold that $2 price point for the reasons cited in this story in the Santa Rosa Press-Democrat. Trader Joe's held the $1.99 price for 11 years, and there are inflationary pressures, including less-than-optimal grape crops in 2010 and 2011.
We understand why they might have to raise the price by 50 cents. Nevertheless, Two-and-a-half-Buck Chuck just doesn't have the same ring.


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Jomo Kenyatta International Airport in Nairobi
Jomo Kenyatta International Airport – this is the main airport in the country. It is located in Nairobi City, about 20km from the central business district.
Jomo Kenyatta International Airport is the main airport in Kenya and the busiest in East Africa. Initially, it was referred to as Embakasi Airport because it is located in Embakasi area, some 20kms north of Nairobi city. It commenced its operations in the late 50s but due to the dramatic increase in commercial and developments activities some years later, there was need to expand it so that it could be able to handle the increased capacity.
The expansion began sometimes in the 70 and in 1978, a new terminal which could handle international flights was built and the name changed to Jomo Kenyatta International Airport which was named after the first founding president of Kenya. Since then, the airport has been expanding as the demand for its use increased and new terminals have been added. There are currently five commercial terminals at the airport all which serve commercial and domestic flights.
Today, the operations at the airport have immensely increased and airports serve about 19000 passengers from Africa, Europe and Asia. It also has direct flights to African countries, Europe, Middle East and Far East and there are plans to construct a new terminal which will enable the operations to have direct flights to USA.
Kenya Airways is the official airline in Kenya and works closely with our international airlines to offers as a competitive edge to the aviation industry.
Below are aerial views of the images of the airport;
East or West Home is the Best
It had been years since I went to Sweden to pursue my degree in Architecture, and I over stayed there since I got a landed a job there, but upon completion of the contract, it was time that I went back home. There had been many expectations that my folks back home had on me, and it was time to prove that I indeed was to live to their hopes as promised.
As I passed through the immigration checkpoint, I had this feeling that I might have overstayed in the Scandinavian countries more that I had promised. I really missed my parent and my siblings and I felt that they too missed me. I wanted to be part of them again after living for 7 years in Diaspora.
There was no hustle in the KLM airlines which was to fly me back to Nairobi. Actually, I had waited for long for such an opportunity to come through. Finally, it was here. I had bags full of luggage – my personal effects and the few things that I had bought for my family back at home. The flight must have taken relatively longer than I had anticipated because I arrived in Kenya two hours to midnight.
The Taste of Nairobi Life
From the looks of things upon arrival, I could tell that much had changed. Of course I expected so since the new Kenya constitution was promulgated and new county government took the management of various regions. However, as I met two of my brother who had been waiting for me to arrive at the airport, I felt as if life was the same way as it used to be. They helped me with the luggage and took me straight to a restaurant that had accommodation in, where they had plan for my stay through the night.
However, I knew very well that after meeting them, there wouldn't be any special night for me even though I was tired. I never had to ask so. Even after I went to take a shower, I left them in the sitting lounge waiting for me and from the look in their eye; I could conclude that they wanted me to share some more time with them. I knew quiet well that I had a lot that they wanted to hear and therefore, I hurriedly showered and after refreshing up, we went out.
Our firsts stop was at the Coffee Java House, where we had some bit of pleasure as we sipped the beautifully prepared Kenyan coffee. Afterwards, it was time for dinner. By this time, it was already late and we couldn't get the specific foods that we had ordered for.
We decided to go to Nairobi west and have some 'nyama choma', which is one of the favorite delicacies of Nairobians. Our first stop was the Carnivore Simba Saloon. I may have said that all we were interested in was eating some 'nyama choma' (roasted meat), but once any person reaches the Carnivore, then it's always time to prepare for the Nairobi nightlife.
Things to do in Nairobi
Actually we partied the whole night and getting up in the morning was a quiet cumbersome. When I finally managed to get up, my brothers surprise me with a visit to the Nairobi National Park. Before I left, the park was well managed, but now their seemed to be a lot of changes. The wildlife crew was much friendly and hospitable, and from the look of things while in the park, the number of wildlife had largely increased.
From the park we visited University of Nairobi, where I wanted to say hi to my kid sister. She was so much pleased to see me because since she was a child she looked up upon me as a sense of inspiration and a mentor.
Before we headed the day or got to get back to schedule our activities, I suggested that we visit Mama Ngina Children's Home. This is one place that I always loved visit during my high school days and I believed that I always inspired the under-privileged kid there in a way. Most of the faces there were knew kids, but it didn't take long for me to get along with them. But the time we were done there, it was time to think about visit the rural areas where my dad and mum lived.


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text: POLO Expressversand, Germany - Constructor Group Workplace Storage Reference POLO Expressversand is amongst the leading suppliers of motorcycle clothing, accessories, technology and sportswear. All orders, from POLO stores and online customers, are handled in this new logistics centre. Solution: The centrepiece of the new POLO logistics centre in Jüchen is made up of 3 four-tier-order-picking towers based on Dexion's Hi280 industrial shelving system – with 10,000 m² of gangways and a height of nearly 10 metres. It represents the first four-tier system of this kind in Germany. Capacities: Warehouse area: 25,000 m²Storage base: 45,000 m²Picking rate: up to 200,000 articles per shift Hi280 rack dimensions: Height 9,5mCommissioning shelves: 57,000Walkway area with grids: 10,000 m²


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Teacher resources and professional development across the curriculum
Teacher professional development and classroom resources across the curriculum
This multimedia course covers content from the Pre-Columbian era through the present day. Each unit covers an era in American history through text materials, video, and classroom activities. For an overview of the series, START HERE.
© Annenberg Foundation 2017. All rights reserved. Legal Policy


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AECWeekly examines select top news each week, picks out worthwhile reading from around the web, and special interest items you might not find elsewhere. This issue will feature Industry News, Top News of the Week, Acquisitions/Agreements/Alliances, Announcements, Awards, New Products, and Upcoming Events.
AECWeekly welcomes letters and feedback from readers, so let us know what you think. Please send me your comments
Best wishes,
Susan Smith, Managing Editor
Industry News
Update on Plant and Process – Part 2 of 2
by Susan Smith
Autodesk Plant Solutions
Last year Autodesk entered the Plant and Process market with Autodesk P&ID and is now talking about a product called Plant 3D to be released later this year.
Vice president of Plant Solutions Mark Strassman stated that the answer to Plant for 25 years has been AutoCAD, with over half a million licensed plant users. Today Strassman said that 10-15% of the AutoCAD user base is using AutoCAD for plant design. Strassman also added that plant works across all the vertical market segments.
|[ Click to Enlarge ]
Plant fits very well into global trends. Since 1950, population growth has been greater than in the previous four million years. Strassman noted that there are 200,000 new people on the earth each day, meaning there are that many more beings with a demand for energy, water, and food.
One of the biggest issues is water, as in the 20th century alone population tripled and water consumption grew six times. Looking ahead, there will be 4 billion people on the planet by the year 2050.
All things are processed in plant, food beverage, water, wastewater, medications, energy consumption and these will all increase 50% in the next five years. Renewable energy sources are growing at about the same rate as eventual energy sources as well.
Strassman said that Autodesk is addressing the plant market at this time because of the increasing world energy consumption, shortened project schedules and regulatory pressure, and reporting and testing.
So many plant designers use AutoCAD and AutoCAD based tools already. Many existing plant tools are hard to use, and the same issues that affect the rest of the industries affect plant:
Staffing crisis.
- 50% designers will retire in 10 years
- subcontracting and offshoring
- cost and time for training
- AutoCAD trained base is largest available
Information needs not met.
- incompatible design systems
- deliverables are drawings
- IT support
Autodesk is one of the only companies that offers what they call "multidisciplinary engineering:" civil, structural, architectural, plant, MEP, equipment, geospatial, power, and controls.
The news is that Autodesk is working on 3DPlant, and working hard to integrate heating ventilation, AC, equipment design, civil design, Buzzsaw, ADT, Revit, and other products. They're looking at having an API where you can hook up to a database, and are working with AVEVA and Intergraph to provide a data layer into their database.
The P&ID product is built like Excel, so that it fits in with existing workflows. The next release of P&ID will focus on rounding out P&ID drawings to specify the logic of the plant, what's connected to what, with a logical diagram to the whole plant including the design and operations and maintenance phase.
P&ID has tag information and line information as to what these different lines are. These are a challenge to keep synchronized, but users can work with their P&ID anywhere and can share a common data structure and share all data with the 3D designer.
Although P&ID only needs to be 2D, there is a lot of back and forth between P&ID and the 3D designer, then after that, the involvement of instrumentation.
Strassman pointed out that up to 80% of designers' time is spent on revision. Currently, Autodesk is working on making the application easier and easier, so that the processes of moving things around and putting them back together and revising take less time. Also, set up and maintenance time needs to be improved "If these issues aren't dealt with people will find less efficient ways to work," Strassman noted.
Strassman said they have been showing a working product of Plant3D and hope to have it out this year. "What we're trying to do is solve a key piece of the puzzle in a way that's flexible. Designers have to make a choice as to a database based system or file based system. With Plant 3D you'll be able to make a choice."
People aren't able to keep track of a big 3D model while doing asset management in plant, for example, and with Plant 3D you could connect it to a database to keep track of it.
Navisworks is useful in plant for visualization and clash detection. Other features of Plant 3D will address how to bring in an Inventor file and what part of that file will be relevant to the plant design.
Correction to last week's Industry News "Update on Plant and Process – Part 1 of 2" –
In the second paragraph of the article, FIATECH is referred to as a consulting firm that is "adding some of the tools for ISO 15926." FIATECH is not a consulting firm. It is an industry consortium housed at the University of Texas in Austin. As such it is a not-for-profit and is driven by its members (Bentley, Intergraph and AVEVA are among them). Information can be obtained by going to the website. FIATECH and the POSC Caesar Association have been working on the implementation of the ISO 15926 standard over the years and members are developing tools to support it. – correction provided by Nicole Testa, deputy director, FIATECH.
Top News of the Week
ShareCG.com now features a growing number of animated architectural fly-throughs, and the caliber of the material is excellent.
Primavera Systems, Inc., released details of its record-breaking 2007 fiscal year (ended Dec. 31, 2007), in which the company capped 24 years of consistent revenue growth. Total revenue for 2007 increased by 43 percent to $176 million.
Primavera's growth in 2007 was primarily driven by its ability to deliver industry-focused solutions that meet customer demand for end-to-end project, program and portfolio management. In recent years, organizations have increasingly adopted company-wide, standardized systems for project, program, resource and portfolio management. Primavera's integrated software suite is the industry's only solution that covers the entire project lifecycle, from prioritization, to program and portfolio management, to risk management and execution.
CH2M HILL, a global full-service engineering, construction and operations firm, announced that it has completed the acquisition of Texas-based Goldston Engineering, Inc. Goldston, with offices in Houston and Corpus Christi, is a technology driven consulting engineering company providing marine, coastal, municipal, and transportation engineering services.
CAFM Resources announced the upcoming release of FMView. FMView provides robust web access to Autodesk FMDesktop data via the browser and includes a comprehensive and innovative user interface that makes it easy to search, theme, edit and report facilities information.
TRIRIGA INC. (" TRIRIGA"), the global leader in Integrated Workplace Management Systems (IWMS), announced that another leading U.S. retailer has selected TRIRIGA's solutions, TRIRIGA for Retail(TM) Real Estate and Projects and TRIRIGA performance management. Toys"R"Us, Inc., the nation's leading toys and baby products retailer, recently purchased the TRIRIGA for Retail and the TRIRIGA performance management solution.


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It was back to the drawing board for the group desiring to petition for the recall of four town trustees, based on their votes against the EPIC downtown theatre project. Dr. Bert Bergland, Greig Steiner and Gail Upp filed the petition with town clerk Jackie Williamson on Friday, naming trustees Eric Blackhurst, Wendy Koenig, Ron Norris and John Phipps as subjects to recall in the petition.
However, Monday, Williamson said she denied the petition in its first form, and sent a letter to Bergland about a wording change. There are certain things in the state statute that must be addressed to ensure the petition is in the right format, she said.
The filers of the petition have no formal name as a committee at this point, Williamson said, and if they want to be known as a committee, they will also have to file paperwork per the Fair Campaign Practices Act.
"We'll see where it heads," she said.
Until there is a formally approved petition, the matter is in a kind of limbo. Until the petition is formally approved, Williamson can't talk about timelines. She said she is not sure exactly how the community would hold a special election, but that it would probably be similar to other elections. The cost could run to $12,000. Mail-in ballots have increased the costs of elections, she said.
There's always the possibility that she could return the petition back to Bergland and the group would decide to do nothing about it. That possibility is slim, though, Williamson said, since they've come this far.
Contact Trail-Gazette Staff Writer Juley Harvey at 970-586-3356 or firstname.lastname@example.com


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City officials throughout California who are fighting for control over massage parlors see opportunity in the future as a controversial state law that regulates and protects the businesses is about to go off the books.
Several believe the law has encouraged illegal activities such as human trafficking and prostitution.
"There are a lot of concerns coming out of local communities, especially San Gabriel, about what has transpired because of the state law," San Gabriel City Manager Steve Preston said. "It didn't completely pre-empt local regulation but it so hamstrung it that a lot of the measures cities would normally use to control it have been taken away."
The law, passed in 2009, established the California Massage Therapy Council, a nonprofit charged with issuing permits for all massage therapists in the state. The law also prohibits cities from restricting legitimate, licensed massage parlors and technicians unless they apply the same regulations to other licensed professional businesses like a doctors or chiropractors.
A number of local jurisdictions have seen the number of businesses jump exponentially since the law was passed; for example, the four-square-mile city of San Gabriel is now home to 50 massage businesses; Thousand Oaks has 47 and Huntington Beach has 74.
Cities from South Pasadena to Gardena, Fontana and Torrance have been vocal on the issue. They have organized stings and arrested massage parlor employees for prostitution in recent years, but many say they don't have the resources to successfully police the illegal businesses. Some cities have passed local ordinances to regulate massage parlors, and others have imposed a temporary moratorium on new businesses, but most say without a change in the law their hands are tied.
The law will sunset in 2015, and the state legislature is in discussions to update it, working with cities and massage parlor owners.
The League of California Cities has a list of changes it hopes to make to the law. League representative Kirstin Kolpitcke said the group wants a state agency to be given regulatory authority rather than nonprofit CAMTC, and that cities be permitted to impose unique restrictions on massage businesses.
"Cities and counties don't uniformly regulate business professionals, they place restrictions on businesses based on the type of use of that business and the problems or benefits that might be associated with that," Kolpitcke said. "Because of that language (in the current law) we can't regulate massage at all."
Kolpitcke said the group would like to see harsher restrictions in the state law for massage licensing, including holding business owners accountable for the actions of their employees. In addition, some say the process to get a license should be made more difficult and include requirements like forcing applicants to undergo a criminal background check.
"In the case of something like human trafficking, they can remove someone's license but the business continues to operate. Nobody is regulating the business itself or the owner," Kolpitcke said. "If I own a grocery store and a cashier sells alcohol to an underage person, not only would the cashier be in trouble but I would also be in trouble because I'm responsible for what goes on in the store."
But cities aren't the only ones looking for a better law, the CAMTC has also been involved in the process and CEO Ahmos Netanel said the agency is on board with harsher regulations to "weed out the bad apples."
"We are proposing modifications to improve the law to make it stronger so cities will have even greater authority to deal with the problem of illegal massage establishments," Netanel said. "We believe we share the concerns of the cities; we understand them fully."
The Assembly's Committee on Business, Professions and Consumer Protection will hold a hearing on the new law Monday. It will be streamed online at abp.assembly.ca.gov.


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Baltimore isn't known as the City of Great Sports Billboards. But perhaps it should be.
A taunting billboard that went up in January, shortly after the Ravens beat the Patriots in the AFC playoffs, was called the "Best Sports Billboard Ever" by columnist Jessica Isner of bleacherreport.com.
The billboard, put up by Clear Channel Outdoor's DC/Baltimore branch on all 19 of its digital billboards in the city, proudly announced that Ray Lewis' retirement party was being put off until Feb. 3 -- not coincidentally, the date of Super Bowl XLVII.
The billboard added, in smaller but still prominent type, "New England not invited."
(For Isner's full list of her 20 "Best Sports Billboards Ever," click here.)
It should be noted that the Baltimore billboard was put up in response to one put up by Clear Channel's Boston branch. On that one, created in anticipation of what New Englanders unfathomably considered a sure Patriots win, a digital clock counted down the days to Lewis' retirement party -- which coincided with the day of the AFC championship game vs. the Ravens.
"Well played, Baltimore," Isner noted. "Well played."
Said Steve Ginsburg, president of Clear Channel Outdoor DC/Baltimore: "We love Baltimore and the Ravens and were happy to donate our creative energy and billboard space to our hometown."
Also last week, a new billboard went up on I-95 south, just before the O'Donnell Street exit, promoting the Charm City Roller Girls, Baltimore's entry in the Women's Fast Track Roller Derby Association. The ad, dripping with bad-girls attitude, was put up by MGH, a local communications agency that sponsored a contest offering to design a free billboard for one small Maryland business.
The billboard will remain up through June 27.
We don't know if the Roller Girls billboard will earn any Internet plaudits, but it definitely attracts attention -- and properly suggests these gals are not to be trifled with.


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Easy Christmas Carols Instrumental SolosVarious Composers - Alfred Music Publishing
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Spread the joy of the holidays with this collection of easy favorite Christmas carols, written especially to encourage beginning musicians. Each of the 14 titles is masterfully arranged, and the CD contains both a demonstration track and an accompaniment track for each arrangement. A separate piano accompaniment book is also available. Songs include: Angels We Have Heard on High; Away in a Manger (Medley); Come, Thou Long-Expected Jesus; Go, Tell It on the Mountain; We Three Kings; Hark! The Herald Angels Sing; It Came Upon the Midnight Clear; Joy to the World; O Come All Ye Faithful; O Come, O Come, Emmanuel; O Little Town of Bethlehem; Silent Night; The First Noel and What Child Is This?.
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New GDP numbers add to old worries
Fresh evidence of a significantly slowing U.S. economy Friday posed new headaches for President Barack Obama's re-election bid – coming on top of weak readings in recent weeks on employment numbers and concerns that the seemingly endless European debt crisis will keep the American economy from getting off the mat.
The economy grew at an annual rate of 1.5 percent in second quarter compared to a 2 percent rate in the first quarter of 2012, the U.S. Department of Commerce's Bureau of Economic Analysis reported on Friday.
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And the report offered little hope for a dramatic uptick in the third quarter – a number that will be released just five weeks before the Nov. 6 election.
( Also on POLITICO: Graph: GDP numbers)
"Nobody is going to be really happy with the economy going into the end of the year," said Mark Zandi, chief economist of Moody's Analytics
The sluggish growth number will only ratchet up anticipation for the new monthly employment numbers that will be released next Friday.
The Romney campaign and congressional Republicans on Friday were quick to jump on the GDP number and point to it as evidence that the president's economic policies have failed. Romney has made charges of Obama's failed economic stewardship the central plank of his campaign to unseat him.
"If we keep up at this rate, over the next year or two, we will simply never get back to full employment," Glenn Hubbard, a top economic adviser to the Romney campaign, told CNBC. "It's not good news."
The Obama administration noted that while the economy is not growing as fast as hoped, there has been a vast improvement since the president took office in 2009 — the message it has been trying to sell to voters.
Then the administration hit back, with White House Press Secretary Jay Carney pivoting off the GDP report to pressure Congress to pass the president's Jobs Act, which he said would create 1 million jobs.
"We're going to keep pressing for action," Carney said, noting that Obama would continue to do so personally. "He is going to continue to hammer the point that we know what we can do right now… to spur job growth."
Alan B. Krueger, chairman of the White House Council of Economic Advisers, highlighted flagging public spending as a cause for the slowing economy and noted the president's plan to send funding to state and local governments to hire teachers, police officers, emergency medical technicians and firefighters.
The data released on Friday showed that federal spending dipped 0.4 percent, while spending by state and local governments dipped 2.1 percent.
"While the economy continues to move in the right direction, additional growth is needed to replace the jobs lost in the deep recession that began at the end of 2007," Krueger said.
But the GDP is a sort of triple-whammy in the key measures many voters use to assess just how the economy is doing. Months of sluggish jobs numbers have made it unlikely Obama will see unemployment dip below 8 percent, a figure it has remained above for 41 straight months, before voters go the polls in November.
"I think GDP growth will be about 2 percent, which is what we've been getting over the past year or so," Zandi said. "Job growth will be 125,000 [to] 150,000 per month through the end of the year, which isn't enough to make significant progress in unemployment."


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Recent News
March 28, 2014
Clif Bar & Company Announces Voluntary Recall of 15-Count Boxes of Chocolate Chunk LUNA® Bars Due to Package Mislabeling
The Missouri Department of Health and Senior Services received information regarding a recall of a small amount of 15-count boxes of Chocolate Chunk LUNA® Bars that were shipped to limited stores nationwide. These 15-count boxes include Chocolate Chunk LUNA® Bars made with macadamia nut butter, but the wrappers do not include macadamia nuts in the ingredient list or allergen statement. The wrappers do contain advisory allergen labeling, which states that the product may contain traces of other tree nuts. Clif Bar & Company is taking this precautionary safety step for people who are allergic to macadamia nuts. People with an allergy to macadamia nuts run the risk of a serious or life-threatening allergic reaction.
The voluntary recall applies to Chocolate Chunk LUNA Bar 15-count boxes or individual bars meeting the following criteria:
Bar UPC: 7-22252-10068-9
15-Count Box UPC: 7-22252-20068-6
'Best By' Dates:
No other LUNA Bar products, pack sizes, flavors or 'Best By' date codes are affected.
The company is strongly advising consumers who have macadamia nut allergies not to consume these mislabeled bars. No allergic reactions have been reported to date.
The full recall can be found at: http://www.fda.gov/Safety/Recalls/ucm390934.htm?source=govdelivery&utm_medium=email&utm_source=govdelivery.


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Explore Mykonos
Welcome to my site. Here you will find exciting mashups of my very own recipes for the everyday home cook. I draw influences from my days working as an Executive Chef at my own Chinese restaurant in Downtown San Francisco, as well as inspiration from various cuisines I experience from all my travels.
Chef Kathy Fang
All tagged Old Port
Explore Mykonos


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Say What? Bank Helps Borrowers Find Work
By Carole Fleck , April 22, 2013 12:07 PM
As shocking as it may seem, an Ohio-based bank is helping some of its unemployed borrowers find jobs. Why, you ask? Because these customers are at serious risk for defaulting on their home mortgages.
Fifth Third Bank, which operates some 1,300 branches in 12 states, has teamed up with employment firm NextJob to offer free job search assistance. It includes one-on-one coaching, online job search software and other resources to mortgage holders who could face foreclosure because they're out of work and can't make their house payments.
[ Read: Picking the right mortgage options]
In a pilot project last year, 28 customers who'd been out of work for an average of 22 months took advantage of the free job search help. Eleven found full-time jobs within six months, Fifth Third spokeswoman Barbara Grimsley says.
With that kind of success, the bank expanded the program in February. Grimsley says Fifth Third Bank is the first financial institution to offer this kind of assistance to mortgage customers.
Maya Gray, a 40-year-old mother of two from Chicago, was one of those customers. She lost her job in 2011. A short time later, she received a bank notice informing her that her mortgage payment was about to increase by $300 a month due to a tax hike that was tied to her mortgage payment. She called bank officials to say she couldn't afford that increase because she was unemployed.
The next day, she says, she received a FedEx package from the bank offering her job assistance. Within six months, she landed a job as a senior project process analyst, which involves process improvements.
"When I got the job offer, I screamed on the phone," Gray says. "I was so thankful. It was just in time financially."
Up to half of the bank's mortgage delinquencies are the result of customers who've lost jobs. So paying an employment firm to help them find work sure beats the average $50,000 to $60,000 it costs to process a foreclosure, Grimsley says.
Wouldn't it be great if other banks followed suit? Then we'd have more working Americans saved from the heartbreak of foreclosure. And a stronger housing market to boot.
Photo: Images_Of_Money/flickr
Also of Interest
- Workers: Here's How to Reinvent Yourself
- 3 Pieces of Jewelry That Make You Look Younger
- Join AARP: Savings, resources and news for your well-being
See the AARP home page for deals, savings tips, trivia and more


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64 Comments
OMG! Meet Robert Pattinson & Kristen Stewart's Daughter!
Isn't she so cute? She totally looks like Rob & Kristen!
While Kristen Stewart is worrying about having to film a graphic birthing scene in The Twilight Saga: Breaking Dawn, casting directors are hard at work, searching for the perfect little girl to play her and Robert Pattinson's offspring, Renesmee. And it looks like they might have found her, as Model Management Group posted the above photo on its Facebook page Sept. 1 with the following caption: "Attached is a photo of Kayla and Daniel D. … Kayla is the little girl up for the role of Bella's daughter in the new film in the Twilight saga, Breaking Dawn."
Obviously she hasn't officially been cast in the movie yet, but we definitely think she could play the part! What do you all think of Rob and Kristen's potential progeny?


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Malou Navio, coordinator of the Antipolo project in the Philippines, sent photos of the destruction left behind by Typhoon Ketsana. The three CFCA projects, Manila, Antipolo and Quezon, impacted by the typhoon are distributing food and water, and assessing the extent of the damage. CFCA has reported no deaths or severe injuries among sponsored members. Below you will see some of the photos from Malou. You can read an update on the situation here.
Sponsors and others wishing to help may donate to CFCAís Disaster Assistance Fund. One hundred percent of donations to this fund are sent to CFCA projects to help individuals and families affected by disasters. Funds donated are used where they are most needed.


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School official cleared in Obama rodeo clown act at Missouri State Fair
A Missouri school official has been cleared of wrongdoing in a State Fair rodeo act that mocked President Barack Obama.
Albert Watkins, an attorney for Boonville superintendent Mark Ficken, said Wednesday that an investigation by the school district found Ficken was not involved in inappropriate actions or statements.
Ficken was an announcer at the rodeo but has said he was unaware a clown was going to rile up the crowd to watch a bull charge a man wearing an Obama mask. Some initial media reports attributed the remarks to the announcer, not the rodeo clown.
The Boonville School District sought an independent review of Ficken's role after the Aug. 10 incident.
Rodeo Clown Tuffy Gessling told KCTV that he was just trying to be funny.


text: True stories, life, encouragementBe Music January 26, 2018 Candy'sCrumbs Share this: Follow me at Candy's Crumbs@FacebookFacebookEmailLike this:Like Loading... Related Published by Candy'sCrumbs I'm just a woman who has discovered that a lifelong dream can come true. I can write, share, possibly inspire and encourage many. My blog is a little bit of this, a little bit of that and a lot of sass! View all posts by Candy'sCrumbs


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The Forex world is constantly evolving and during its weekly trading hours there is a wealth of news a trader must keep on top of if they want to make the most money possible. Indeed, because the market can be affected by a number of dynamics, it is essential they know exactly what's happening at all times in order to avoid any unprofitable deals.
For example, let's assume the US Dollar is trading well against the Great British Pound during the first half of the week. This would be a good time to invest some money and reap the benefits of a positive market condition. However, by Wednesday a series of negative news stories emerge from the UK and affect the country's economic performance. These dynamics then adversely affects how well the two currencies are trading. At this point it's important to know what's just happened because it will influence whether or not someone should cease trading this currency pair for a while or continue.
Knowing the value of each currency based on various internal and external factors is crucial and the reason why FXBrokerSearch.com's Forex news centre is a vital resource. Drawing on a number of resources, our information section provides the latest information and data from which users can refine their trading activity.
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Facebook faces a class-action lawsuit over its use of facial recognition technology to tag people in photos. According to the judge's order, damages could rack up to billions ..
Before airing George Stephanopoulos' interview with former FBI Director James Comey, ABC News made the unusual decision of releasing a full transcript of its nearly five-hour ..
ZTE Corp. may have just gone from being a serious contender in the high-stakes world of next-generation networking to -- quite possibly -- a mobile industry washout. ..
SHANGHAI/BEIJING (Reuters) - A move by the United States to ban American firms selling components to the Chinese telecom equipment maker ZTE Corp will also hit a target closer ..
Having a spouse heading into retirement might make your golden years more fulfilling. However, here's how you can live an enriching life in retirement--even if you're single. ..
A Republican strategist leapt to the defense of President Donald Trump following comments made by former FBI Director James Comey at the weekend. ..
Brian Klaas, fellow in comparative politics at the London School of Economics, discusses former FBI Director James Comey's comments about President Donald Trump. ..
At first glance, Pfizer Inc. (NYSE:PFE) appears a worthwhile contrarian trade. Additionally, the overall markets have been soft, allowing shrewd investors to pick up PFE stoc ..
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Risk Disclosure: fxBrokerSearch.com will not be held liable for any financial loss or damage caused by users acting upon any information contained within this website, not limited to and including: all numerical data, quotes, charts and buy/sell signals. Moreover, please be advised that Forex trading is one of the most volatile investment forms in the world and all trades should be placed with full consideration of the risks and costs.
fxBrokerSearch.com does not support nor encourage the execution of any investments. Trading with a margin is high risk endeavour and not suitable for everyone, therefore, each investor should carefully consider all relevant trading conditions, such as experience, risk and cost, before taking part in any type of trading, including Forex.
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I apologize if this question is not up to par. When I was doing exercises in basic mechanics I checked the answers and I can't seem to find what I'm doing wrong. Suppose we have a ball with mass $m$ and radius $r$ on an inclined plane with height $h$. At the end of the inclined plane is a loop with a radius of $R$ and we can assume that $r<<R$. We are asked what the minimum height is the inclined plane should have so as to let the ball complete the loop. Here is my reasoning:
We have $U_1\geq K_{rot,2}+K_{trans,2}+U_2$.
For the ball rotating we have $I=\frac{1}{2}mr^2$ and $\omega=\frac{v}{r}$.
So $mgh\geq \frac{1}{2}I\omega^2+\frac{1}{2}mv^2+mg\cdot2R=\frac{3}{4} mv^2+mg\cdot 2R$.
The minimal speed to complete the loop implies $F_{centripetal}=\frac{mv^2}{R}= mg$
So $v^2= Rg$ and we have $gh\geq \frac{3}{4}Rg+g\cdot 2R$ which means $h\geq 2\frac{3}{4}R$, while the book says that the answer should be $h\geq 2.70R$. Can you explain what I am doing wrong?
Thank you
EDIT: Moment of inertia corrected.
For the ball rotating we have $I=\frac{2}{5}mr^2$ and $\omega=\frac{v}{r}$.
So $mgh\geq \frac{1}{2}I\omega^2+\frac{1}{2}mv^2+mg\cdot2R=\frac{7}{10} mv^2+mg\cdot 2R$.
After the edit with correction of the moment of inertia I am getting the right answer. We get $gh\geq\frac{7}{10}Rg+g\cdot 2R$ so $h\geq2\frac{7}{10}R$ in accordance with the book.


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Join Shana Mahaffey as she tours the blogosphere December 6, 2010 to January 14, 2011 to promote her new women's fiction novel, Sounds Like Crazy (Penguin Books).
Though she doesn't remember the trauma that caused it, Holly Miller, the main character of Shana's novel, has Dissociative Identity Disorder. Her personality has fractured into five different identities, together known as The Committee. And as much as they make Holly's life hell, she can't live without them. Then one of those identities, the flirtatious, southern Betty Jane, lands Holly a voiceover job. Betty Jane wants nothing more than to be in the spotlight. The rest of The Committee wants Betty Jane to shut up. Holly's therapist wants to get to the bottom of her broken psyche. And Holly? She's just along for the ride.
The San Francisco Chronicle says, "Mahaffey documents a realistic setting and progression of psychotherapy…both funny and poignant, Sounds Like Crazy celebrates resilience as an essential element of the human condition."
"Mahaffey has infused the book with keen insights into human nature and the complexities of life that challenge all of us," says San Franscisco Book Review. "Sounds Like Crazy is a novel that should appeal to anyone who has ever wondered about the little voice in the back of their head."
Tish Cohen, author of The Inside Out Girl, says "Tender, fresh, and darkly comic, Sounds Like Crazy is a sweet and poignant debut. In exploring the reality of a lonely woman living with dissociative identity disorder. Shana Mahaffey has created an honest and compelling character that will both touch your heart and have you laughing out loud."
Shana Mahaffey lives in San Francisco in an Edwardian compound that she shares with an informal cooperative of family, friends and five cats. She's a survivor of Catechism and cat scratch fever, and is a member of the Sanchez Grotto Annex, a writers' community. Her work has been published in SoMa Literary Review and Sunset Magazine. She welcomes all visitors to her website www.shanamahaffey.com, and is happy to meet with book groups in-person or in cyberspace (phone/webcam/the works).
If you'd like to find out more about Shana during her virtual book tour, visit her official tour page here.
You can purchase Sounds Like Crazy at Amazon.


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Age Group
Your age on 31 DECEMBER of the year the race takes place determines your age group (meaning the athlete will start in the same age group throughout the calendar year)
Minimum Age: 18 years
You have 2 different options...
A - You have a valid triathlon license
B - You do not have a triathlon license
Avoid last minute stress and read attentively the column below corresponding to your license situation:
A - You have a 2020 valid license from a national triathlon federation
Add your license and photo identification to your "bring along checklist" right away. During on-spot race registration, you will have to present them to the registration team together
B - You do not have a 2020 license from a national triathlon federation
During the on-spot race registration, you will have to buy a one-day-license from the FITRI (Italian Triathlon Federation).
In order to receive your FITRI one-day-license you need to:
* Bring a medical certificate for the practice of triathlon. In order to meet all legal requirements, the medical certificate must be issued by an authorized Italian center for sports medicine, NOT OLDER THAN 1 year on the race day and with the compulsory mention: "for triathlon".
* Present a valid photo identification
* Pay 30 € for Age-Group athletes (cash only)
According to Italian law and the regulations, a medical certificate is compulsory to obtain a sports license in Italy. These obligations apply to every organizer of any kind of competition and all sports. To meet the needs, we therefore recommend, to register to your national triathlon federation and to bring your membership card with you at the Registration Office
We recommend to all athletes to keep a copy of your medical certificate with you, you may need it for another competition.


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Get an extension of factory warranty coverage beyond the original factory warranty term for peace of mind and protection from failure
The Extended Warranty enables repair or replacement of your single phase UPS and accessories (PDU) for an additional 1 to 3 years. It includes several additional benefits, like 24/7 Customer Service support, Service Entitlement support and next-business-day shipment of replacement units.
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Bile test for Lactobacillus
Posted 05 August 2012 - 11:50 PM
I'm a student currently doing my Final Year Project and I needed help for biochemical tests on detecting the presence of Lactobacillus from a sample.
My project involves isolating the bacterias from a sample (fermented beverage) and the aim is to find/ detect the presence of Lactobacillus in it by doing biochemical tests, and molecular methods.
One of the tests is by using Bile salts test to detect the probiotic properties of the colonies that was isolated ( colonies that are picked are rod shaped and gram positive).
My question : When I prepared 0.4% of bile salt solution in MRS broth and distributed to 19 bijou bottles for 19 samples and I autoclaved it after that. Somehow, after autoclave, the solution turns out to have white precipitate in each of the bottles (the bottles are clean and washed) instead of how it was originally in cloudy form. Is it normal for it to be precipitated like that after autoclave? The solutions have to be autoclaved since my work involves bacteria it is to avoid any possible contamination. I was thinking of autoclaving the MRS broth first then add the bile salts later. Is it wise?
After inoculating the bacteria into the bottle with bile solution, the cultures are let to grow and readings are taken after 6hours and 24hours using the UV-Vis spectrophotometer. Since the precipitates present after autoclaving, I fear that it might interfere with the readings.
Hope that you could help. Thank you =)
Posted 06 August 2012 - 01:38 PM
It is possible that you get precipitations, not everything can be autoclaved, so sometimes you need to filter sterilize it.
Not sure about this one though.
MRS itself should not be a problem, so I guess its the bile salts.
I know there are some media out there with bile salts that can not be autoclaved, but I doubt its because of the salts.
What you were thinking is good: autoclave the MRS first, check for precipitations (if there is none, then its the bile salts thats cuasing it). After autoclaving it, let it cool down and add the bile salts using a filter in the laminair flow.
Make sure to work sterile.
(its the same with adding antibiotics : you also add those after autoclaving the medium and letting it cool down)
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.
Posted 06 August 2012 - 06:55 PM
MRS broth recipe containing phosphate and MgSO4 such the one preformulated by Difco (Difco™ Lactobacilli MRS Broth) can have slightly opalescence aspect, ie becomes hazy/whitish after autoclaving. Tween80 can change the appearance of the medium specially right after leaving the autoclave, but usually become clearer when cooling.
Don't know if bile salts may affect. In all media I saw they are added prior autoclaving but they are old formulations
As said by pito, try first to see the aspect of normal broth. If it still has that aspect, try to add the MgSO4 (and maybe MnSO4) after autoclaving if you want a clear broth. MgSO4 is added after autoclaving in many minimal media (as well as CaCl2) because they use to precipitate probably by forming phosphates.
Posted 09 August 2012 - 05:41 AM
friend I think the same,of Veteran's answer. Since bile salts contain steroid nucleus, they gets destroyed upon autoclaving. like plant hormones we use. (your doubt about interference is reasonable) So you better add them after the Autoclaving. with in the laminar air flow(use flame if possible to maintain strict sterile condition during addition) to avoid precipitation.
Posted 09 August 2012 - 10:10 AM
Yeah, it is just MRS with bile salts that had been dissolved in distilled water prior addition to the MRS.
Thank you for your idea, I shall try it out by using the filter. =)
Um, there's one more thing, is it okay if i filter the bile salts solution (without autoclaving) after dissolving it with distilled water? Will it make any difference to the cloudiness of the solution? I noticed that the solution will turn quite cloudy and the reading for the spectro could not be obtained as it is too cloudy.
El Crazy Xabi,
Yeah, the precipitates appear like a cloudy medium, and there's some that precipitates so badly that white clumps presents too.
The type of MRS that I am currently using is Merck's and it does contain phosphate but it does not turn cloudy after autoclaving. So I guess it must be the bile salts that's causing it.
Thank you so much for the information, I shall take it as a note =)
Hi =)
Oh, I see.. so if the bile salts solution is autoclaved then the chemicals that it contains will be gone? So that means it will be the same just like not putting in any bile salts into the MRS? LOL
Thank you for your advice =)
Posted 09 August 2012 - 04:17 PM
Reading the Difco manual of culture media (you can download it from the web) many culture media with bile salts are not autoclaved
For example for the TCBS agar, it says:
1. Suspend 89 g of the powder in 1 L of purified water. Mix
2. Heat with frequent agitation and boil for 1 minute to
completely dissolve the powder.
3. Cool to 45-50°C and use immediately. DO NOT AUTOCLAVE.
4. Test samples of the finished product for performance using
stable, typical control cultures.
However, few others like the EC medium (and variations), MacConkey agar (if it will be used after more than 12 h, also variations) are autoclaved but seems to be exceptions:
1. Dissolve 37 g of the powder in 1 L of purified water. Mix
2. Warm slightly to completely dissolve the powder.
3. Dispense into tubes containing inverted fermentation vials.
4. Autoclave at 121°C for 15 minutes.
5. Test samples of the finished product for performance using
stable, typical control cultures
So, my recomendations:
- If use will be inmediate (and you are lazy ) just heat up the medium to disolve if required.
- If you are willing to used the next day, willing to store some or like me, you are more confident sterilizing everything, autoclave the MRS broth and filter sterilize a stock of bile salts and add it after cooling the medium
Posted 10 August 2012 - 07:32 AM
And yes, you can dissolve or dilute the bile salts with water , just use (to be save) autoclaved water and then filtersterilise the bilesalt dilution.
About being too cloudy: I dont know, never had this kind of problem before. If it turns cloudy when adding water.. then doesnt it turn cloudy when you add it to your broth too..?
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.
Posted 11 August 2012 - 08:32 AM
Thanks! I will tyr out the method that you suggested =) Thank you so much again
Autoclave the water..sounds a great idea. Thank you!
Yup, the bile salts will turn cloudy too when it is put into the MRS broth. I was thinking of reducing the cloudiness to that the reading from the spctro could not be affected. But I wasn't so sure if there's such a way to reduce it. =/
Posted 12 August 2012 - 01:07 PM
EL Crazy Xabi
Thanks! I will tyr out the method that you suggested =) Thank you so much again
Autoclave the water..sounds a great idea. Thank you!
Yup, the bile salts will turn cloudy too when it is put into the MRS broth. I was thinking of reducing the cloudiness to that the reading from the spctro could not be affected. But I wasn't so sure if there's such a way to reduce it. =/
The reading of the spectro could still be ok? You normally measure first without any micro-organisms...
If the entire solution is cloudy (or more dark) then its ok..
(there only problem is when you have pieces floting around.. if you see what I mean... )
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.
Posted 12 August 2012 - 04:48 PM
Is the product new? I've been checking some bile salts product sheet in Sigma and... (example of bile salts mixture B3426):
The Bile salts mixture is a white, free flowing,
fine powder. It is freely soluble in water and
forms a clear, colorless solution that
produces foam if shaken. When incorporated
into culture media, the bile salts mixture does
not affect the color of indicator dyes or their
subsequent color change.
Bile Salts: ³45% (expressed as cholic acid)
Moisture: £6.0%
Final pH: 7.0 ± 0.5 (1% Solution)
Bile salts B3301
powder freely dissolves in water. The aqueous solution is clear and yellow,
and produces foam when shaken. The solution remains clear after
Moisture: Not more than 6.0%.
Effective Concentration: 0.25% - 0.5%
Final pH of a 1% Solution: 7-9
Also, in one of the products specifies max solubility of 10%.
So what you are explaining seems pretty weird. I'm not saying that you lie, don't missunderstand me, but it seems that the product doesn't match the expected characteristics. Check your product code and manufacturer specifications to be sure
Posted 27 September 2012 - 10:38 PM
sorry for replying this late as i've been away
so does that means if there's pieces of bile precipitations at the bottom of the MRS solution it will give a not-so-good spectro result(if i understanded it correctly lol)?
btw, i can't get my hands on the sterile filter. but with the sediments in the MRS, i was wondering if i could just proceed, or replace the MRS with nutrient broth? it's because nutrient broth does not have any probs with the bile solution.
El Crazy Xabi
no, it's no problem. yea you're right. i made a mistake there. when i mentioned that it stay cloudy in water it's because i didn't fully waited for it to dissolve in water, and it does stays cloudy if it was kept stirred.
i've checked the pH for a 10% bile solution (1g of bile in 10ml of water) and it's about pH 8.
it will turn the MRS cloudy once the bile is mixed into the MRS solution as the MRS solution is at pH5 (the brand of MRS that i'm using is Merck's)
my Supervisor suggested that i should change the pH of the bile mixed with MRS solution to pH 7 so that the cloudiness disappear (it did actually) as it might be the difference between the bile solution pH level and the MRS pH level. but i was wondering if that could affect the solution/environment that is as same as it is before i alter the pH level.
plus, when i autoclaved the bile mixed with MRS (pH that had been changed to 7) it didn't turn out with precipitates like how i attached a picture of it previously, but it had a little 'sediments'-kind of precipitates at the bottom.
so here ate the pictures attached.
first pic : (left to right) MRS broth before adding with bile salt solution, a 0.2% bile in MRS, a 0.4% in MRS, and 10% bile solution.
second pic : 0.2% and 0.4% bile in MRS with pH 7 after autoclave. looks way better compared to the ones previously. but there's some 'sediment'-like at the bottom.
extra. third pic : since my lacto's not really stable in growing in MRS, i've been using nutrient broth as a substitute (since it is a standard requirement for bacteria growth broth. i think). the lacto grew well in it and i've thought of replacing the MRS with nutrient broth for the Bile test. is it wise?
Edited by Tan Mei Yin, 27 September 2012 - 10:44 PM.
Posted 01 October 2012 - 06:42 PM
Also, measure the pH after autoclaving, it might change and be another reason for the precipitates.
Nutrient broth is quite different from MRS so, I would suggest to keep the MRS if possible. But check it with your supervisor
Posted 03 October 2012 - 02:07 AM
once the bile is added in, all i saw was cloudiness. and when it was autoclaved with the MRS, the cloudiness became a white clump. (this was before i changed the MRS pH level to 7.)
and i've tried another time by changing it exactly to a 10% bile solution pH level which is at pH8.36. the sediments dissolved completely and have no problems even after autoclaving.
so i guess it might be the pH's influence like how you've said it..(my supervisor said that she did it before but have encountered no problems like this when she autoclaved her MRS with bile salts. i think it might be the different MRS broth brands?)
thanks for suggesting =)
i was just wondering if it could affect the result since its pH had been changed to 8.36. wouldn't it will be the same as without adding bile into the MRS, but with the condition that the MRS pH is changed to pH8.36?..as in with or with out the bile salts, the pH of the MRS will still be 8.36.
man, i'm a lil but confused. sorry =/
Posted 04 October 2012 - 12:00 AM
The media with lowest pH I've seen is 6.9, most others are from 7.0-7.5. I would recommend to keep the pH as low as possible for the growth of Lactobacillus.
The pH change of MRS-bile salts pH7 might be because of the MRS or the bile salts interaction with the medium. So, I would consider 2 options to get, maybe, the desired pH:
- Test the initial pH of the media+bile salts to achieve a final pH 7 after autoclaving. Just make some mixture, dispense in tubes and adjust the pH individually in intervals of 0.2 units of pH (should be precise enough). Check the pH of the tubes after autoclaving and cooling. One will definitelly have the desired pH.
- MRS (adjusted pH) and bile salts autoclaved separately. Hopefully the buffer capacity of MRS will avoid big pH changes. You may try adjust the pH of the bile salts before, but I don't know what will happen... You also can mix the solutions, adjust pH and then filter sterilize, but that's a pain
Hope you find a solution soon
Posted 05 October 2012 - 01:53 PM
sorry for replying this late as i've been away
so does that means if there's pieces of bile precipitations at the bottom of the MRS solution it will give a not-so-good spectro result(if i understanded it correctly lol)?
btw, i can't get my hands on the sterile filter. but with the sediments in the MRS, i was wondering if i could just proceed, or replace the MRS with nutrient broth? it's because nutrient broth does not have any probs with the bile solution.
Cant you get any flters? Normally labs have these.. I would find it weird you dont have acces to it...
And yes, the sediment will indeed influence the results.. you should not have this sediment. Or you can try to get rid of the sediment... Just just the supernatans. But not sure the bacteria will grow good enough then, but you can try it.
If it is a sediment, changes are high that the bacteria cant even use it.. if it is complexed then the bacteria cant use it as food anyway, so try the supernatans.
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.
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President's Son Tries to Retract Remark
Abhijit Mukherjee, the son of Indian President Pranab Mukherjee and a member of Parliament from West Bengal, is under fire for remarks criticizing women who have been protesting the recent gang rape of a New Delhi student. The protests have attracted a range of Indian men and women but have been characterized as led by students.
In an interview with a Bengali news channel, Mr. Mukherjee said: "Those who claim to be students - I can see many beautiful women among them - highly dented-painted (sic) - they're giving interviews on TV, they've brought their children to show them the scenes. I have grave doubts whether they're students, because women of that age are generally not students."
He said it's become fashionable to attend protests, candles in hand.
The comments prompted a swift and fierce backlash from politicians across the spectrum and sparked outrage on social media. "Dented-and-painted", by some accounts, is slang for someone who has tried very hard to look good and has put on lots of makeup. "Dented" refers to a car dent that has been fixed.
Mr. Mukherjee told NDTV news channel that he "withdraws those sentences" that offended people and apologizes "unconditionally." He said he would never have made the remarks if he knew it would have caused such a firestorm. Asked about protesters who say it is their right to be "dented and painted," he agreed, saying, "It's their right."
S. Sunil, an official in Mr. Mukherjee's office, told India Real Time that Mr. Mukherjee's comments were misconstrued. "It was twisted and blown out of proportion," he said. "He has every respect for everyone. He regrets if he has hurt somebody. He himself is very hurt by this unnecessary controversy."
His sister, Sharmistha Mukherjee, told Bengali news channel ABP that her brother's remarks were out of line. "Honestly speaking, I am really shocked," she said. "Whether these were students or not students doesn't matter. This anger is completely justified."
Thousands of people poured into the streets of central Delhi and the area surrounding the India Gate monument last weekend, protesting the government's inability to crack down on crime against women. Many are demanding stricter punishment for convicted rapists and fast-track courts to process sexual assault cases. There are some 40,000 rape cases pending nationally in India, experts say.
The 23-year-old woman who was beaten and raped on a bus in New Delhi on Dec. 16 was moved from the city's Safdarjung Hospital to a medical facility in Singapore. She remained in critical condition Thursday.
Amol is an India Correspondent for The Wall Street Journal. Follow him on Twitter @AmolSharmaWsj.


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It's Ryan Gosling's Birthday! Watch 9 Essential 'Mickey Mouse Club'-Era Baby Goose Moments
I don't care if you're already sick of the blogosphere's fawning, today is Ryan Gosling's birthday and that is practically an internet holiday. (Not to mention an actual one. Shout out to the veterans out there.) And unlike you Gosling latecomers out there who jumped on the Baby Goose train after The Notebook, some of us have been faithful fans for almost two decades now, and that kind of lifelong dedication warrants an entire post full of internet videos, okay?
more »


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Mennonite neighbors sharing Christmas
Tonight, two of our neighbor families came to our house Christmas caroling. They are Mennonite families, and this is is the second Christmas that they've sung for us, so perhaps it will be a tradition. They told me that they visit six to eight homes in the neighborhood.
Together, the two families had (more or less) a dozen children with them. With most of the children and all the adults singing, their little choir has quite a few voices. They sang "O Little Town of Bethlehem," "We Three Kings of Orient." and another that I was not familiar with.
As it happens, both of these families have had a disagreement with the local Mennonite church and no longer attend there. It is interesting that both husbands have become licensed drivers and have bought vehicles since leaving the church. They arrived at the house tonight in a couple of vans.
I noticed that one of the mothers no longer wears her little white cap. Her hair is in a long single braid down her back, not pinned up on her head anymore. It's surely easier and more comfortable for her.
Beyond that, I have no idea what changes leaving the local church has brought to their lives, but I am glad that they feel we are their friends. It was really nice to have them stand in our kitchen and sing for us.


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Wow - what a pimp. Seven posts since you've been here and six of them contain links to your kitesurfing business. I guess is beats paying for ads.Hooked-onKitesurfing wrote:In the UK its so expensive. When you go on kitesurfing holidays to other places its usually a lot cheaper. SO top tip there! go on holiday to fix your kite!
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A fractured Manchester City side patched together a 3-3 draw at home against Sunderland yesterday thanks in part to two Mario Balotelli goals. Yet City management find themselves most concerned with Balotelli's on-pitch behavior, especially in light of this tantrum he threw yesterday over not being allowed to take a free kick (one eventually squandered by Aleksandar Kolarov).
City manager Roberto Mancini dismissed Balotelli's two goals, saying "he didn't play well" and suggested he considered substituting for Balotelli just five minutes into the match. We're not in the practice of offering advice that anyone in charge would never read—plus, you know, we like when Mario Balotelli does things—but if you're this angry with a player who saved a point for your team during a title race, it seems somebody's priorities are severely misplaced. [ESPN2]


text: With some people actually getting their hands on the Audeze LCD-2 planar magnetic headphone, I thought it's time for a thread of positive impressions only. No objectivity needed in here. To my ears, it has an extremely natural and life-like presentation. It has a rather unique sound signature, with deep low bass that's presented in detail, without any trace of bloat or boom. At the same time, it has sparkly detailed highs that are just there without being too loud. All of it is presented in a well-defined 3D headstage. An amazing musical experience, that can also be fully enjoyed at low volumes. Other LCD-2 threads: Amplification for the LCD-2 General discussion of the LCD-2 Cables for the LCD-2 First version Typical frequency response chart The most recent look, with some cosmetic changes


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Playing Dress-Up: The Yankees' Halloween Costumes
"Witch and ghost make merry on this last of dear October days. " – Author Unknown
In one week's time, people will be dressing up in costumes for Halloween, the one day on the calendar you can be whoever or whatever you want to be. Whether it's a super hero, a movie character, or a spooky creature, Halloween is one of the more celebrated holidays in this country.
To me, Halloween has sort of lost its luster. When I was a youngster I absolutely loved Halloween. It was always a tossup trying to figure out what I should dress up as, and the thought of getting free candy just by uttering the phrase "Trick-or-Treat" put a smile on my face and got me excited.
In first grade I dressed up as Superman, one of my favorite costumes:
It was always a night of fun for me. Yet these past few years I've found myself basically doing nothing on Oct. 31, merely sitting around alone watching old horror movies like "The Exorcist" and "Scream."
This year I might change that, perhaps; maybe try to rally some friends together and relive the days of Halloween past by donning costumes. But onto the point of this entry:
If the Yankee players were to dress up for Halloween, what could they be?
Here are some suggestions…
CC Sabathia: Cleveland Brown
I could have just taken the high road and suggested CC Sabathia dress up as Fat Albert, but I figured I would change it up this time.
Put a yellow shirt and blue pants on the Yankee ace and he just might be able to pull it off. However, he might need to grow his mustache in a little more.
Hmm. Maybe Derek Jeter's father, Dr. Charles Jeter, would be better off going as Cleveland…?
Derek could even go as Quagmire!
Mark Teixeira: Jared from Subway
I don't know about anyone else but if you take the glasses off Jared, I think he sort of resembles Mark Teixeira.
Sort of.
The Yanks' first baseman, who batted just .248 this year, might be able to get one of those "Free Subway for Life" cards (Happy Gilmore reference) if he goes as Jared this year. Maybe some meaty, cold cut sandwiches can help him swing the bat a little better and get that BA back up to a respectable .300 next year.
Scott Proctor: John Cena
I know I've made mention of this in the past but Scott Proctor, who went 0-3 with a 9.00 ERA for the Yankees this year, looks an awful lot like 12-time WWE Heavyweight Champion John Cena.
Proctor was basically a real-life loser this season. Therefore, if he wants to dress up like a real-life winner, he can be Cena for Halloween.
All he would need for the costume is one of Cena's colorful shirts, a hat that reads "You Can't See Me," some wrist bands, and a pair of denim shorts.
Oh, and maybe some talent. That wouldn't hurt.
A.J. Burnett: Beavis
A.J. Burnett pitched a decent Game Four in the American League Division Series vs. the Tigers, but only had a handful of acceptable starts during the regular season. With a record of 11-11 and an ERA of 5.15, he hasn't exactly been what the Yankees were anticipating when they signed him to a lucrative contract worth $82.5 million prior to the 2009 season.
But we already knew that.
Perhaps he can help get the Yankee fans back on his side if he loosened up a little bit and dressed as Beavis from MTV's…umm…hit show, "Beavis & Butthead."
Not only do they bare a striking resemble to one another, their personalities are eerily identical. And if he doesn't like the Beavis idea, he can always go as Dennis the Menace.
Andruw Jones: Dr. Dre
I'm not sure why, but Andruw Jones has always reminded me of rap star and hip-hop mogul Dr. Dre. When both of them were young, they were well-liked and everyone knew their name.
Dr. Dre was one of the more revered rappers in the world and most people knew Jones because of the power show he put on in the 1996 World Series as a member of the Atlanta Braves.
But that was in the 1990s. If you were to ask anyone back then who Dr. Dre was, undoubtedly they'd say, "Of course!" Nowadays if you ask about him, the answer would probably be more along the lines of, "Oh yeah. Whatever happened to him?"
Same applies to Jones.
And while we all know what Jones was doing this year – batting .247 for the Yankees and hitting 13 homers with 33 RBIs as a fourth outfielder – we may never know where Dr. Dre is.
I guess we all "forgot about Dre." I believe that was one of his last hit singles; I can remember him releasing it before he vanished off my radar somewhere around 1998.
Alex Rodriguez: Anakin Skywalker
The Yankees' superstar third baseman had a sad 2011 season, spending most of it on the disabled list. Hurt, he only managed to club 16 homers, bat .276, and drive in just 62 runs.
It was one of the worst years Alex Rodriguez has had since he started; in fact, it was the first time in his career he failed to hit 30 home runs since 1997.
To get rid of some of the bad feelings from this past season, I could picture A-Rod dressing as Anakin Skywalker from the Star Wars franchise. Not only are they practically each other's doppelganger, but it fits so well.
For those who do not know, Anakin Skywalker became Darth Vader, one of the sworn leaders of the "Dark Side." The Yankees have always been compared to the Star Wars "Evil Empire" so who better than Rodriguez – probably the most hated Yankee – to play the Jedi who turns his back on the force for the sake of evil?
It's a perfect match and it makes sense.
Bartolo Colon: Hamburglar
I have an unsettling admission to make right now. One of the last years that I went out trick-or-treating on Halloween (probably in sixth or seventh grade) I was indeed the Hamburglar, one of the McDonald's "McDonald-Land" characters.
Yep. Insert obligatory/witty Chicken McNugget joke here.
Bartolo Colon, just judging by his face, would be perfect for this costume. In fact, I wouldn't be surprised if during the off-season he makes appearances at local McDonald's in the Dominican Republic as the Hamburglar for extra money.
Also, judging by his weight, it looks as though he might be the real-life Hamburglar. He is very porky.
Robinson Cano: Steve Urkel
Back in the early 1990s ABC launched its Friday night lineup known as TGIF. Shows such as "Step by Step," "Boy Meets World" and "Full House" all kept us laughing – albeit with cheesy, light-spirited, and family-oriented jokes.
One of my favorite programs to watch every Friday night was "Family Matters," a show centered around the lives of the Winslow family and their pesky, annoying, nerdy neighbor, Steve Urkel.
Robinson Cano is no nerd: 28 home runs, 118 RBIs, and a .302 batting average is nothing to shake your head at. It certainly does not put him in the same category as Urkel.
However, if you take Cano's face, put some coke bottle glasses on him, some suspenders, a nerdy shirt, and if he speaks with a high-pitched squeaky voice, you have the spitting image of the legendary doofus known as the Urk-Man.
Well, that about wraps it up folks. Hope you enjoyed my suggestions for the Yankees' Halloween outfits.
If you happen to go out next week, be safe, and Happy Halloween.


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