text: "No! no!" said Bergan, turning hurriedly away, "it is impossible. You mean kindly, I know, Maumer Rue, but you do not seem to understand the facts. I have no more right to it than any stranger; I could not touch it, to save me from starving. Come, let us go! I have seen enough." Chapter 10 FEELING HIS WAY. Nevertheless, Brick loved his young master with all his heart. He had succumbed at once to the rare charm of Bergan's manner,攕o grave and thoughtful for his years, yet so richly illuminated, at times, with soft gleams of humor, and always so genuinely kind. He followed him like his shadow; he could scarcely be happy out of his presence; and notwithstanding his own inward struggles with doubt and mortification, he continually held him up to the admiration of the quarter in the strongest language of encomium that he could command, as a "bery high-tone gemman, and jes' de bes' massa dat ebber stepped foot on de old place." The two cousins stole out of the room together. In the studio, Diva put her arms around Astra and kissed her tenderly. 日本毛片高清免费视频_日本阿v片在线播放免费_日本黄大片免费 ARREST OF SIR FRANCIS BURDETT. (See p. 597.) Paine, in his "Rights of Man," was far from restricting himself to the courtesies of life in attacking Burke. He had been most hospitably received by Burke on many occasions at his house, and had corresponded with him, and must therefore have seen sufficient of him to know that, though he might become extremely enthusiastic in his championship of certain views, he could never become mean or dishonest. Yet Paine did not hesitate to attribute to him the basest and most sordid motives. He branded him as the vilest and most venal of apostates. Paine had, in fact, become a monomaniac in Republicanism. He had been engaged to the last in the American Revolution, and was now living in Paris, and constantly attending the Jacobin club. He was hand-in-hand with the most rabid of the Republicans, and was fast imbibing their anti-Christian tenets. Paine fully believed that the French were inaugurating something much finer than any millennium; that they were going to establish the most delightful liberty, equality, and fraternity, not simply throughout France but throughout the world. Before the doctrines of the French clubbists and journalists, all superstition, all despotism, all unkindness were to vanish from amongst mankind, and a paradisiacal age of love and felicity was to commence. To those who pointed to the blood and fury already too prominently conspicuous in this business, he replied that these were but the dregs of corrupt humanity, which were working off in the great fermentation, and all would become clear and harmonious. But far more remarkable were the effects of the championship of French principles in the celebrated Dr. Joseph Priestley. Priestley was now nearly sixty years of age攁 time of life when men rarely become great enthusiasts in any cause. He was a Unitarian minister, and was now the pastor of a congregation at Birmingham. He was well known for various theological writings, in which he had announced his doubts of the immateriality of the sentient principle in man, especially in his "Disquisition on Matter and Spirit." He had been tutor to Lord Shelburne, first Lord Lansdowne; but had quitted that post, as supposed, in consequence of the objection of Lord Shelburne to these principles, retaining, however, an annuity of one hundred and fifty pounds a-year. But Priestley was far more known and esteemed for his researches and discoveries in natural philosophy, especially in electricity, chemistry, and pneumatics. Orthodoxy and Toryism were extremely rampant in Birmingham, and Priestley was regarded as the very patriarch and champion of Socinianism and Republicanism. There wanted only a spark to fire trains of fierce intolerance against Priestley and his party, and, unfortunately, this was furnished by themselves. They resolved to celebrate, by a dinner, the anniversary of the taking of the Bastille, on the 14th of July. Before the dinner took place, such were the rumours of impending riots that the party proposed to defer the celebration to a future day; but the landlord had prepared the dinner, and declared his opinion that there would be no danger if the party dispersed early, without stopping to drink many toasts. Darbley, the innkeeper, curiously enough, was a Churchman, and in good odour with the Tory party. Satisfied by his representations, about eighty persons determined to hold the dinner on the appointed day, though a considerable number stayed away, and amongst those Priestley himself. The company were hooted as they entered the inn, but chiefly by a crowd of dirty lads, who cried "Church and King!" On the table were ranged three figures: a medallion of the king encircled with a glory, an emblematical figure of British Liberty, and another of French Slavery bursting its chains. In the evening a fierce riot broke out, instigated攁ccording to Priestley's account攂y some prominent magistrates, though the statement was never proved. The mob rushed to Darbley's hotel after the dinner was over and most of the people were gone. There they raised the cry of "Church and King!" and began to throw stones. Some one cried out, "Don't break Darbley's windows; he is a Churchman!" But the Church-and-King people and their set, now flushed with wine and loyalty, waved their handkerchiefs from the windows of the opposite inn, and hurrahed the mob on. With this encouragement, which seemed to the crowd to legalise their proceedings, the mob rushed into the house, declaring that they wanted to knock the powder out of Dr. Priestley's wig. They did not find the doctor, so they smashed most of the furniture in the house, and dashed in the windows, notwithstanding the host's orthodoxy. Some one then cried, "You have done mischief enough here; go to the meetings!" and the mob rolled away, first to the new meeting-house, where Priestley preached, which they soon demolished and set fire to. They then proceeded to the old meeting-house, and destroyed that too, being hounded on by people of decent station in the place, and made furious by the beer which was distributed among them. Five days after this, February 10th, the matter was made public by Lord Darnley rising in the Upper House, and moving for an inquiry into the conduct of the Ministry. This roused up Lord Grenville, who candidly avowed that, in consequence of their failure to introduce the question of Catholic emancipation, the Ministers had resigned and only held office till a new Cabinet was formed. On this, Lord Darnley postponed his motion. On the same day, in the Commons, a letter from Addington, the Speaker, was read, announcing his resignation of the Speakership in consequence of the king's proposal to nominate him to a situation incompatible with that post. Pitt then rose and confirmed this, and proposed an adjournment till the next day in order to prepare for the nomination of the new Speaker. The House adjourned accordingly, and next day, the 11th of February, elected Sir John Mitford, the Attorney-General, as Speaker. Before the House could resume business, it was announced that the king was ill攃onfined to the house by a severe cold; but it was soon known that it was a return of his old malady, lunacy, in consequence of his extreme agitation on the proposal of the Catholic question and the resignation of Pitt. The report was soon augmented into the startling rumour that the king was dangerously ill, and that a regency must take place攊f not superseded by his death. At this news Fox, who had for some time absented himself from Parliament, on the plea that all endeavours to carry sound and prudent measures were hopeless with Pitt's great martial majority, hastened up to town from St. Anne's Hill; and the Whig body was in a flutter of expectation that he would soon be the Minister of the prince regent, or of George IV. But all these hopes were speedily overthrown by the news of the rapid improvement of the king, and on the 12th of March the royal physicians announced him perfectly recovered. He attributed his illness to Pitt's conduct, and the ex-Premier thereupon wrote and promised never to re-open the question again. We may well imagine that Jonas, with his 渟trong sense of religion,?was one of that earnest band of 渟everal hundreds of plain people?who listened, spellbound, to the eloquence of John Wesley on that memorable day of May 1742, on which, on Birstall Hill, began the great Yorkshire 淩evival.?


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Pedro José Sanches Filho
Instituto Federal de Educação Ciência e Tecnologia Sul-rio-grandense, Brazil
Title: GC/MS application in the determination of lipids in archaeological artifacts samples
Dr Pedro José Sanches Filho graduated in Full Degree in Chemistry and graduate in Pharmacy (1988), master's degree in Chemistry from the Federal University of Rio Grande do Sul (1997) and a PhD in Chemistry from the Federal University of Rio Grande do Sul (2002) and post doctorate from the Nova University of Lisbon (2007). He is currently Professor and Researcher at the Federal Institute of Education, Science and Tecnolgia sul-rio-grandense, leader of the Research Group on Environmental Contaminants.
The connection of chemistry as a science with conservation-restoration and historical research of cultural property is of great importance, that because these materials (artifacts, buildings, etc.) are able to store chemicals that allow information both cultural aspects (actions anthropic) as non-cultural (vegetation, and others). The necessity of methods to extract, identify and quantify chemical compounds stored in archaeologies samples such as ceramics, accurately and precisely, makes the gas chromatography coupled to mass spectrometry (GC/MS) to appear as an important analytical tool. This work aims at the optimization methodologies by GC/MS for characterizing lipids found in archaeological artifacts (ceramic fragments) obtained in Cerritos located in the Pampa biome, in the southern portion of the Patos`s lagoon, southern Rio Grande do Sul-Brazil. The methodology is based in a solvent extraction of lipids under ultrasound, clean up by micro silica column and derivatization with MSTFA (N-methyl-N-trimethylsilyltrifluoroacetamide) and analysis by GC/MS. A better characterization, with clarification of several isomeric structures, was obtained in 4.0 g of sample and 30.0mL eluent (CHCl3:CH3OH 2:1 v/v)during the passage in the micro-column with silica. The study allowed us to optimize the methodology for extraction, clean up, pre concentration, identification and quantification of lipid compounds (fatty alcohols, fatty acids, triglycerides) in samples of ceramic artifacts, and identify contamination in the samples in the stages of collection and storage. The results obtained from this method will enable a more accurate and precise correlation between chemical data and the behavior of indigenous pre colonization cultures.


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Cheddar cheese predicted to develop into different quality classes has been evaluated by time domain Nuclear Magnetic Resonance, Thermogravimetric analysis and quantitative sensory analysis. The water and fat proton signals in the transverse relaxation decay curves have been deconvoluted. Proton transverse relaxation values for both the water and fat fractions decrease and the relative %age of the proton peak area, predominantly from the fat increases over a 450-day ripening period. The thermodynamic free water percentage increases during maturation. Water and fat attributes can distinguish between Cheddar cheese batches after 56â¯days. Cheese batches which have lower transverse relaxation values for the water and fat proton fractions and a higher relative %age of the proton peak area predominantly from fat at 56â¯days, mature after 270â¯days to be more yellow, rubbery and smooth, have a less sour and lingering aftertaste and are also harder to form into a cheese ball.
AssuntosQueijo/análise , Gorduras/análise , Manipulação de Alimentos , Água/análise , Espectroscopia de Ressonância Magnética , Paladar , Tempo
This study was designed to evaluate the baking performances of 25 edible dry bean (Phaseolus vulgaris L.) varieties and to investigate correlations among cookie features and rapid test indices (i.e., water and lactic acid retention capacities, oil binding capacity and Rapid Visco Analyzer indices). Two bean powder particle sizes (≤0.5â¯mm, ≤1.0â¯mm) were investigated. Cookies were evaluated in terms of nutritional, geometrical and textural properties. Bean powders doubled the amount of cookie protein and increased cookie resistant starch content. Baking potential varied according to bean genotype and powder particle size: coarse powders resulted in larger (+26%) and thinner (-19%) cookies characterized by easier breaking texture (fracture strengths of 41-157 vs. 48-226â¯kPa for fine powders). Water retention and oil binding capacities and pasting properties significantly (pâ¯<â¯0.05) correlated with cookie features. In conclusion, these accumulated findings can be used in designing value-added traditional and gluten-free cookies.
AssuntosPhaseolus/química , Pós/química , Culinária , Dieta Livre de Glúten , Farinha , Ácido Láctico/análise , Ácido Láctico/química , Valor Nutritivo , Tamanho da Partícula , Amido/análise , Água/análise , Água/química
Two dry surfaces can instantly adhere upon contact with each other through intermolecular forces such as hydrogen bonds, electrostatic interactions and van der Waals interactions1,2. However, such instant adhesion is challenging when wet surfaces such as body tissues are involved, because water separates the molecules of the two surfaces, preventing interactions3,4. Although tissue adhesives have potential advantages over suturing or stapling5,6, existing liquid or hydrogel tissue adhesives suffer from several limitations: weak bonding, low biological compatibility, poor mechanical match with tissues, and slow adhesion formation5-13. Here we propose an alternative tissue adhesive in the form of a dry double-sided tape (DST) made from a combination of a biopolymer (gelatin or chitosan) and crosslinked poly(acrylic acid) grafted with N-hydrosuccinimide ester. The adhesion mechanism of this DST relies on the removal of interfacial water from the tissue surface, resulting in fast temporary crosslinking to the surface. Subsequent covalent crosslinking with amine groups on the tissue surface further improves the adhesion stability and strength of the DST. In vitro mouse, in vivo rat and ex vivo porcine models show that the DST can achieve strong adhesion between diverse wet dynamic tissues and engineering solids within five seconds. The DST may be useful as a tissue adhesive and sealant, and in adhering wearable and implantable devices to wet tissues.
AssuntosAdesividade , Adesivos/química , Coração , Pulmão , Próteses e Implantes , Estômago , Molhabilidade , Resinas Acrílicas/química , Animais , Quitosana/química , Reagentes para Ligações Cruzadas/química , Dessecação , Gelatina/química , Coração/anatomia & histologia , Hidrogéis/química , Ligações de Hidrogênio , Pulmão/anatomia & histologia , Pulmão/química , Camundongos , Ratos , Eletricidade Estática , Estômago/anatomia & histologia , Estômago/química , Suínos , Fatores de Tempo , Água/análise , Água/química , Dispositivos Eletrônicos Vestíveis
The aim of this study was to determine how often rural farmers in a watershed use no-tillage systems combined with crop rotation, contour farming and agricultural terraces. The study area was Paraná Watershed III (PB3) in the western region of Paraná State, and data from the 2006 Agricultural Census of the Brazilian Institute of Geography and Statistics were used. A frequency distribution analysis of farms as a function of the no-tillage (NT) area was conducted in combination with a cluster analysis of soil and water conservation practices (adoption of crop rotation, contour farming and agricultural terrace practices). The results showed that the farms in PB3 adopt adequate soil and water conservation practices, with 73% adopting NT combined with at least 2 other conservation practices; however, agricultural terracing was found to be the most neglected practice in the region. In addition, based on the soil and water conservation practices in the watershed, 5 groups of farms were identified, the worst of which, those located in the municipalities that mainly neglect conservation practices, live in areas with highly erodible soils.
AssuntosAgricultura/métodos , Conservação dos Recursos Naturais/métodos , Produtos Agrícolas , Solo/química , Brasil , Água/análise , Abastecimento de Água
In this paper, 329 Landsat images combined with the Deeply Clear Water Extraction Index were applied to delineate boundaries of Hongjiannao Lake during 1986-2018. The net shoreline movement (NSM) and linear regression rate (LRR) achieved by Digital Shoreline Analysis System (DSAS) were employed to depict the distance and rate change of lake shorelines. Based on the waterline method and lake boundaries, the water levels were derived from ASTER GDEM V2. Water volume variations were evaluated using the combination of lake area and water level. The variations in Hongjiannao Lake can be grouped into three stages: (i) The lake area, water level, and volume variations slightly declined from 57.25 km2, 1211.15 m, and - 0.0220 km3 in 1986 to 56.36 km2, 1210.66 m, and - 0.036 km3 in 1997, respectively. The average degradation distance (NSM) and rate (LRR) of lake shorelines were 74.26 m and 3.48 m/a, respectively. Although these three aspects slightly decreased, they maintained a stable high level due to stability of natural factors. (ii) A rapid decrease in these three aspects during 1998-2015 was expressed by rates of - 1.15 km2/a (the total decrease was - 21.72 km2), - 0.18 m/a (the total decrease was - 3.45 m), and - 0.0068 km3/a (the total decrease was - 0.1419 km3), respectively. The average shrinkage distance (NSM) and rate (LRR) of lake boundaries were 1049.35 m and 55.00 m/a, respectively, and gradually intensifying human activities were the leading factor. (iii) These three aspects increased from 31.75 km2, 1207.03 m, and - 0.1852 km3 in 2016 to 36.19 km2, 1207.23 m, and - 0.1883 km3, respectively, in 2018. The average enlargement distance (NSM) and rate (LRR) of lake shorelines were 196.87 m and 67.85 m/a, respectively, mainly caused by closing of small mines, sluicing activities, and increase in annual precipitation.
AssuntosMonitoramento Ambiental/métodos , Lagos/análise , Imagens de Satélites , Água/análise , Abastecimento de Água
Although indispensable, significant uncertainty still exists in the underlying processes of the formation, dynamics, and emission of greenhouse gases (GHGs), the critical elements needed for the accurate estimation of greenhouse gas fluxes in inland lakes and reservoirs. Seasonal changes in water thermal stratification and turbulence strongly influence the concentration and emission of dissolved GHGs in water columns. Here, we studied the stratification and overturn processes of water column in the subtropical Lianhe Reservoir during different seasons and determined the dynamics of dissolved CO2, CH4, and N2O in the reservoir. Observation of temperature and analysis of chlorofluorocarbons (CFCs) clearly suggested that stratification of water column occurred in summer, but not in winter. The results showed that while dissolved oxygen (DO) was high in the top 5-m layer (the upper epilimnion layer), it dropped considerably especially below 10 m, resulting in an increase in concentration of CO2 and CH4. The high concentrations of dissolved N2O and CH4 were related to the decomposition of organic matter in the hypolimnion layer under anaerobic conditions after stratification. In winter overturn period, vertical circulants of water not only homogenized the concentration of DO in the water column, but also potentially moved CO2, CH4, and N2O from the bottom to the surface of the reservoir. The estimated GHG flux from the reservoir was - 7.13 mmol m-2 day-1 in summer and 2.14 mmol m-2 day-1 in winter. There was the potential that CO2 fluxes from subtropical lakes and reservoirs are overestimated by traditional geochemical models.
AssuntosMonitoramento Ambiental/métodos , Gases de Efeito Estufa/análise , Oxigênio/análise , Água/análise , Dióxido de Carbono/análise , Lagos/análise , Estações do Ano
Cuticular wax accumulation in plants contributes to drought tolerance. Here, we compared the drought levels on two varieties with different genotypes in turnip (Brassica rapa var. rapa) and found that the drought tolerance was higher in the waxy KTRG-B48a than in the wax-free KTRG-B48b. A combination of PacBio and Illumina sequencing analyses revealed that differential transcripts were mainly enriched in the wax synthesis pathway, and a splice variant (BrrWSD1-X2) was identified in the waxy KTRG-B48a. BrrWSD1-X2 had a stronger ability to synthesize wax esters than BrrWSD1-X1 using heterologous expression in yeast (Saccharomyces cerevisiae) mutant H1246a. Then, we speculated that the T to C transversion of the third intron and the higher number of TA repeats in the third intron of BrrWSD1 DNA in the waxy KTRG-B48a may result in a lower efficiency of splicing recognition of the third intron, resulting in the emergence of BrrWSD1-X2 in waxy varieties.
AssuntosBrassica rapa/fisiologia , Ésteres/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Ceras/metabolismo , Brassica rapa/genética , Secas , Processamento de RNA , Água/análise , Água/metabolismo
Ochratoxin A (OTA) is a nephrotoxic mycotoxin naturally found in a wide range of food commodities throughout the world. Aspergillus carbonarius is the most important source of OTA in food commodities such as wine, grapes and dried vine fruits and is also responsible for the formation of OTA in coffee. The aim of this study was to determine the simultaneous effect of three culture media (Czapek Yeast Extract Broth (CYB); Synthetic Grape Juice Medium (SGM) and White grape juice (WGJ)) at three water activity (aw) levels (0.90; 0.95 and 0.98-0.99), and three incubation temperatures (15⯰C, 25⯰C and 35⯰C) on the growth and OTA production by 16 strains of A. carbonarius. The strains were mainly isolated from grapes from areas with a Mediterranean climate. All the strains were confirmed for identity by sequencing of the calmodulin gene. The assay was performed in microtiter plates, determining the absorbance at 530â¯nm and the concentration of OTA after 1, 2, 4 and 10â¯days of incubation. No significant differences were observed in absorbance values between the strains. The highest absorbance values were recorded in CYB at 0.99 aw and at 0.95 aw after 10â¯days of incubation at 25⯰C and 35⯰C. None of the strains were able to grow at 0.90 aw and 15⯰C in any culture media after 10â¯days of incubation. OTA concentration was statistically higher at 15⯰C than at 25⯰C or 35⯰C. The highest significant OTA values were obtained at 0.98-0.99 aw and the best culture media for OTA production was CYB, followed by WGJ and SGM. While strains isolated from Mediterranean climate foods had a similar behavior despite being isolated from different geographical areas, OTA concentration produced by one Robusta coffee strain from Thailand was statistically higher at 25⯰C than at 15⯰C. This would suggest that the type of food matrices and consequently the adaptation of A. carbonarius strains to different climatic conditions would have a greater influence on the ecophysiology of the strains than only their geographical origin.
AssuntosAspergillus/crescimento & desenvolvimento , Aspergillus/metabolismo , Meios de Cultura/farmacologia , Micotoxinas/biossíntese , Ocratoxinas/biossíntese , Aspergillus/patogenicidade , Clima , Meio Ambiente , Microbiologia de Alimentos , Temperatura Ambiente , Tailândia , Vitis/microbiologia , Água/análise , Vinho/microbiologia
Antibiotic compounds in natural waters are normally present at low concentrations. In this paper, an easy and highly sensitive screening method using graphene oxide-functionalized magnetic composites (GO@NH2@Fe3O4) combined with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was established for twelve quinolone antibiotics. GO@NH2@Fe3O4 composites were utilized as adsorbents for magnetic solid-phase extraction. This method combines the advantages of magnetic solid-phase extraction and MALDI-TOF MS, which allows for fast detection of quinolones at low concentrations. To improve absorption efficiency, the following parameters were individually optimized: sample acidity, extraction time, amount of adsorbent used, eluent used, and desorption time. Under the optimum conditions, the established method gave a low detection limit of 0.010 mg/L and allowed the high-throughput screening of twelve quinolone antibiotics (enoxacin, norfloxacin, ciprofloxacin, pefloxacin, fleroxacin, gatifloxacin, enrofloxacin, levofloxacin, sparfloxacin, danofloxacin, difloxacin, and lomefloxacin). The proposed method, having an easily prepared sorbent with a high affinity for quinolones and a convenient, high-throughput detection step, has been shown to have merit for the detection of antibiotics in water samples. Graphical abstract Schematic illustration of the (A) preparation of GO@NH2@Fe3O4 and (B) operating procedure for the MSPE and MALDI-TOF MS detection of QNs.
AssuntosAntibacterianos/análise , Grafite/química , Quinolonas/análise , Extração em Fase Sólida/métodos , Poluentes Químicos da Água/análise , Antibacterianos/isolamento & purificação , Limite de Detecção , Imãs/química , Modelos Moleculares , Quinolonas/isolamento & purificação , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Água/análise , Poluentes Químicos da Água/isolamento & purificação
Polycyclic aromatic hydrocarbons (PAHs) are universal organic pollutants in the agro ecosystems in China, therefore, it is important to understand the uptake and accumulation of PAHs in crops growing on PAHs contaminated soils for human health risk assessments. Water management is a common practice to maintain high grain yields during wheat production. However, the effects of soil water content on the accumulation and translocation of PAHs in wheat are still not clear. The main objectives of the present study were to investigate the effects of soil water content on the accumulation of three selected PAHs (Σ3PAHs, phenanthrene, anthracene and pyrene) in wheat during whole plant growth stage and on translocation or remobilization of Σ3PAHs from vegetative tissues to wheat grains. Winter wheat (Triticum aestivum cv. Xiaoyan22) were grown on Σ3PAHs spiked soils maintaining 80%, 60% or 40% water-holding capacity during the whole plant growth stage. Plant samplings were performed at jointing, anthesis or maturity stage, respectively. The present study showed that grain yield and biomass of the crop increased with soil water content increasing. Transpiration rate of wheat leaf under 80% and 60% water-holding capacity treatments was significantly (pâ¯<â¯0.05) higher than that under 40% water-holding capacity treatment at both anthesis and filling stage. Soil water content and plant growth stage had significant (pâ¯<â¯0.0001) effects on concentrations of phenanthrene, anthracene and pyrene in winter wheat. When exposed to 0, 15, 60, and 150â¯mgâ¯kg-1 Σ3PAHs in soils, Σ3PAHs concentrations in the grains under 60% water-holding capacity treatment were 46.6%, 69.9%, 89.5% and 81.7% of those under 80% water-holding capacity treatment, respectively. The highest concentrations of Σ3PAHs in the crop were recorded at anthesis stage. The distribution of PAHs in different tissues of wheat varied among different soil water treatments and plant growth stages. The present study indicated that optimizing soil water content during winter wheat production could apparently reduce concentrations of Σ3PAHs in grains via influence root uptake of Σ3PAHs and translocation of Σ3PAHs from stem or leaf into grain, suggesting the potential of water management to cope with PAHs contamination in crops growing on PAHs contaminated soils.
AssuntosAntracenos/análise , Fenantrenos/análise , Pirenos/análise , Solo/química , Triticum/química , Água/análise , Antracenos/metabolismo , Transporte Biológico , Biomassa , China , Grão Comestível/química , Grão Comestível/crescimento & desenvolvimento , Grão Comestível/metabolismo , Fenantrenos/metabolismo , Folhas de Planta/química , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Pirenos/metabolismo , Estações do Ano , Triticum/crescimento & desenvolvimento , Triticum/metabolismo , Água/metabolismo
Nonylphenols (NP) are ubiquitous in the environment and show toxic effects and estrogenic activity. According to the corresponding framework directive in the field of water quality, isomers of NP (including 4-n-NP and branched 4-NP) are classified as priority hazardous substances and are regulated as a group of chain and ring isomers with a maximum acceptable concentration of 2 µg/L in inland and other surface waters. This study presents a new sensitive and innovative screening approach for estrogen active NP based on high-performance thin-layer chromatography. NP were focused in a single target zone on thin-layer plates by planar solid phase extraction (pSPE) and detected by a planar yeast estrogen screen (pYES) on the basis of their estrogenic activity. The mean limits of detection and quantitation were 14 and 26 ng per zone, respectively. After liquid-liquid extraction of water samples with dichloromethane, the mean recovery was close to 100% (relative standard deviation of 21% or less), and estrogen active NP were detectable down to 1 µg/L. Thus, pSPE-pYES provides both the detection and the quantitation of estrogenic NP in surface waters at the maximum acceptable concentration. Application of the approach on extracts of surface waters showed the use of pSPE-pYES for environmental samples, and no complex and time-consuming clean-up of the extracts was required. Estrogenic NP were not detectable in any of the investigated surface waters by means of the screening approach presented.
AssuntosDisruptores Endócrinos/análise , Fenóis/análise , Poluentes Químicos da Água/análise , Cromatografia em Camada Delgada/métodos , Disruptores Endócrinos/efeitos adversos , Monitoramento Ambiental/métodos , Fenóis/efeitos adversos , Extração em Fase Sólida/métodos , Água/análise , Poluentes Químicos da Água/efeitos adversos , Leveduras/efeitos dos fármacos
In higher plants, seed size is an important parameter and agricultural trait in many aspects of evolutionary fitness. The loss of water-deficiency-induced crop yield is the largest among all natural hazards. Under water-deficient stress, the most prevalent response to terminal stress is to accelerate the early arrest of floral development and, thereby, to accelerate fruit/seed production, which consequently reduces seed size. This phenomenon is well-known, but its molecular mechanism is not well-reviewed and characterized. However, increasing evidence have indicated that water-deficient stress is always coordinated with three genetic signals (i.e., seed size regulators, initial seed size, and fruit number) that decide the final seed size. Here, our review presents new insights into the mechanism underlying cross-talk water-deficient stress signaling with three genetic signals controlling final seed size. These new insights may aid in preliminary screening, identifying novel genetic factors and future design strategies, or breeding to increase crop yield.
AssuntosSementes/citologia , Água/metabolismo , Secas , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Sementes/genética , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Estresse Fisiológico , Água/análise
BACKGROUND: Plants activate defense mechanisms to cope with adverse environmental conditions, leading to the accumulation and / or depletion of general and specialized metabolites. In this study, a multiplatform untargeted metabolomics strategy was employed to evaluate metabolic changes in strawberry fruit of cv. Camarosa grown under osmotic stress conditions. Liquid chromatography-mass spectrometry (LC-MS/MS) and gas chromatography-mass spectrometry (GC-MS) data from strawberries grown under two water-deficit conditions, irrigated at 95% crop evapotranspiration (ETc) and 85% ETc, and one excess salt condition with a 80 mmol L-1 NaCl solution, were analyzed to determine treatment effects on fruit metabolism. RESULTS: Multivariate principal component analysis, orthogonal projections to latent structures - discriminant analysis (OPLS-DA), and univariate statistical analyses were applied to the data set. While multivariate analyses showed group separation by treatment, T-tests and fold change revealed 12 metabolites differentially accumulated in strawberries from different treatments - among them phenolic compounds, glycerophospholipids, phytosterols, carbohydrates, and an aromatic amino acid. CONCLUSION: Untargeted metabolomic analysis allowed for the annotation of compounds differentially accumulated in strawberry fruit from plants grown under osmotic stress and non-stressed plants. The metabolic disturbance in plants under stress involved metabolites associated with the inhibition of reactive oxygen species and cell-wall and membrane lipid biosynthesis, which might serve as osmotic stress biomarkers. © 2019 Society of Chemical Industry.
AssuntosFragaria/química , Frutas/metabolismo , Cromatografia Líquida , Fragaria/crescimento & desenvolvimento , Fragaria/metabolismo , Frutas/química , Frutas/crescimento & desenvolvimento , Cromatografia Gasosa-Espectrometria de Massas , Metabolômica , Pressão Osmótica , Cloreto de Sódio/análise , Cloreto de Sódio/metabolismo , Espectrometria de Massas em Tandem , Água/análise , Água/metabolismo
Disinfection byproducts (DBPs) generated by ballast water treatment have become a concern worldwide because of their potential threat to the marine environment. Predicting the relative DBP concentrations after disinfection could enable better control of DBP formation. However, there is no appropriate method of evaluating DBP formation in a full-scale ballast water treatment system (BWTS). In this study, multiple regression models were developed for predicting the dibromochloromethane (DBCM) and bromoform (TBM) concentrations produced by an emergency BWTS using field experimental data from ballast water treatments conducted at Dalian Port, China. Six combinations of independent variables [including several water parameters and/or the total residual oxidant (TRO) concentration] were evaluated to construct mathematical prediction formulas based on a polynomial linear model and logarithmic regression model. Further, statistical analyses were performed to verify and determine the appropriate mathematical models for DBCM and TBM formation, which were ultimately validated using additional field experimental data. The polynomial linear model with four variables (temperature, salinity, chlorophyll, and TRO) and the logarithmic regression model with seven variables (temperature, salinity, dissolved oxygen, pH, turbidity, chlorophyll, and TRO) exhibited good reproducibility and could be used to predict the DBCM and TBM concentrations, respectively. The validation results indicated that the developed models could accurately predict DBP concentrations, with no significant statistical difference from the measured values. The results of this work could provide a theoretical basis and data reference for ballast water treatment control in engineering applications of emergency BWTSs.
AssuntosPoluentes Químicos da Água/análise , Poluição da Água/estatística & dados numéricos , Purificação da Água/métodos , China , Desinfecção/métodos , Oxidantes , Oxirredução , Reprodutibilidade dos Testes , Salinidade , Navios , Trialometanos/análise , Água/análise
Angola is one of the countries with a high rate of waterborne diseases, due to the scarcity and poor quality of water for human consumption. The watercourses are receptors of many effluents, mainly domestic sewage, due to a precarious or inexistent sanitation system and a small number of wastewater treatment plants. Therefore, this study aims: (i) to evaluate the water quality (physicochemical and microbiological parameters) of three Angolan rivers (Kwanza, Bengo and Dande) in locations where water is used as drinking water or abstracted for human consumption; (ii) to develop a new water quality index able to quantitatively express the water quality in those sites; and (iii) to assess the spatial distribution of water pollution through principal component analysis (PCA). Water quality assessment was performed by conducting four field surveys (campaigns I to IV); the first two campaigns took place in the dry season, while the last two ones took place in the rainy season. In the first two campaigns, the water quality was suitable to be treated for the production of drinking water, while in the last two campaigns, the water was unsuitable for that purpose (high levels of faecal coliforms were detected). The water quality index allowed to classify the water as generally excellent (campaigns I and II) and poor (campaigns III and IV). The rudimentary disinfection usually performed by individual water suppliers may improve the water quality, but it was not enough to achieve the parametric values required for human consumption in the rainy season (campaigns III and IV) except for Bengo sites. PCA identified sampling sites with the same water quality patterns, grouping into four groups (Kwanza sites) and two groups (Dande and Bengo sites). Therefore, the results of this study may support decision-makers as regards water supply management in the river stretches under study. The new developed Water Quality Index can support decision-makers in terms of water supply management, especially in countries with a high rate of waterborne diseases (e.g. Angola).
AssuntosÁgua Potável/microbiologia , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análise , Angola , Água Potável/química , Fezes/química , Humanos , Chuva , Rios/química , Rios/microbiologia , Saneamento , Estações do Ano , Esgotos/análise , Águas Residuárias/análise , Água/análise , Microbiologia da Água , Poluição da Água/análise , Qualidade da Água , Doenças Transmitidas pela Água
The cooling of the Earth's climate through the effects of anthropogenic aerosols on clouds offsets an unknown fraction of greenhouse gas warming. An increase in the amount of water inside liquid-phase clouds induced by aerosols, through the suppression of rain formation, has been postulated to lead to substantial cooling, which would imply that the Earth's surface temperature is highly sensitive to anthropogenic forcing. Here we provide direct observational evidence that, instead of a strong increase, aerosols cause a relatively weak average decrease in the amount of water in liquid-phase clouds compared with unpolluted clouds. Measurements of polluted clouds downwind of various anthropogenic sources-such as oil refineries, smelters, coal-fired power plants, cities, wildfires and ships-reveal that aerosol-induced cloud-water increases, caused by suppressed rain formation, and decreases, caused by enhanced evaporation of cloud water, partially cancel each other out. We estimate that the observed decrease in cloud water offsets 23% of the global climate-cooling effect caused by aerosol-induced increases in the concentration of cloud droplets. These findings invalidate the hypothesis that increases in cloud water cause a substantial climate cooling effect and translate into reduced uncertainty in projections of future climate.
AssuntosAerossóis/análise , Aerossóis/química , Mudança Climática/estatística & dados numéricos , Atividades Humanas , Modelos Teóricos , Temperatura Ambiente , Água/análise , Água/química , Poluição do Ar/análise , Efeito Estufa/prevenção & controle , Efeito Estufa/estatística & dados numéricos , Chuva , Incerteza
Commission Regulation (EC) 543/2008 limits moisture and protein contents in poultry meat. However, this regulation leaves room for interpretation regarding sample homogenisation, potentially affecting comparability of laboratory results. Therefore, a proficiency test and sample homogenisation study were organised amongst 19 European National Reference Laboratories (NRL). In the proficiency test, three different pre-homogenised chicken samples (fillets, drumsticks and carcasses) were analysed. Only one NRL produced unsatisfactory results. In the homogenisation study, NRLs were supplied with uniform fillet, drumstick and carcass materials. Homogenisation was performed according to the NRLs in-house methods. Five NRLs did not return satisfactory results. As these NRLs produced satisfactory results in the proficiency test, their increase in z-scores was related to their homogenisation practices. Overall, scattering of individual results was higher for drumsticks compared to fillets and carcasses. Homogenisation practices for poultry meat introduced significant differences in moisture and protein results and standardisation is therefore advisable.
AssuntosAnálise de Alimentos/normas , Aves Domésticas , Proteínas/análise , Água/análise , Animais , Laboratórios , Carne/análise , Padrões de Referência
Various daily human activities can result in the release of pollutants of different chemical constituents and specific gravities into natural soils. Pollution of natural soils is a recurring occurrence in the environment and it contributes greatly to the alteration of soils properties. The results of an assessment of the effects of selected petroleum-derived and vegetable oils on soil physicochemical and hydraulic properties are presented in this work. Topsoil samples at a depth of 0-20 cm of the same textural class were collected from the order Lixisols and Nitisol within Ogun State, southwest Nigeria. Surface soil samples were collected and treated with petrol, diesel and palm oil at two different volumes (50 and 100 ml). Investigated soil properties include particle size distribution, soil pH, bulk density (BD), total porosity (TP), saturated hydraulic conductivity (Ksat), available water capacity (AWC), total carbon (TC), total nitrogen (TN), organic matter (OM) content, cation exchange capacity (CEC), potassium, sodium, and soil resistivity. Analysis of variance and Pearson's correlation were used to study the variations of the relationship of analyzed soil properties under different soil types and treatments. The regression analysis shows that all the generated models for predicting Ksat values under different soil treatments had R2 values ranging from 0.999 to 1.000. Results showed that treatment with either petroleum-derived or lipids has no effects on soil pH and textural class. Results further revealed that palm oil contamination at 50 ml recorded least values of Ksat in the two soil types. In all cases, BD and Ksat of the contaminated soils of the two sampling locations were reduced compared with their control values. Correlation coefficient showed expected strong negative correlation between TP and BD as well as between any two of organic parameters (TC, TN, and OM) and soil resistivity, TC, and TN at 1% level in both soil types. Two-way ANOVA showed that there were significant differences at 5% level between the two locations with respects to BD, TP, and CEC while significant differences in Ksat, pH, TC, TN, and OM occur between soils from the two locations under various treatments at 5% level.
AssuntosMonitoramento Ambiental , Petróleo/análise , Poluentes do Solo/análise , Carbono/análise , Humanos , Nigéria , Nitrogênio/análise , Óleos Vegetais/análise , Solo/química , Água/análise
Using Granier-type thermal dissipation probes (TDP), we measured stem xylem sap flow of the natural dominant species Quercus liaotungensis and a reforestation species Robinia pseudoacacia from July to September in 2016 in the semiarid loess hilly region. Meteorological factors and soil water content were simultaneously monitored during the study period. Using cross-correlation analysis, time lag between diurnal patterns of sap flux density and vapor pressure deficit (VPD) was quantitatively estimated. Differences in the time lag between the two species and possible influence by different diameter classes and soil water contents were analyzed. The results showed that the diurnal courses of sap flux density were similar to those of meteorological factors, with daily peaks ear-lier than VPD. The peak of VPD lagged behind the sap flux densities of Q. liaotungensis and R. pseudoacacia 118.2 min and 39.5 min, respectively. The peak of PAR lagged behind the sap flux density of Q. liaotungensis 12.4 min, but was 68.5 min ahead of that for R. pseudoacacia. Time lag between sap flux density and VPD significantly varied between tree species and was affected by soil water content. Those during higher soil water content period were about 32.2 min and 68.2 min longer than those during the period with lower soil water content for the two species, respectively. There was no correlation between time lag and tree diameter classes. The time lag between VPD and sap flux density for R. pseudoacacia was about 21.4 min longer in smaller diameter trees than in larger trees, which was significantly different under the lower soil water content. Our results suggested that the time lag effect between VPD and sap flux densities in the two species reflected their sensitivities to driving factors of transpiration, and that higher soil water content was favorable to sap flux density reaching its peak early. The lower soil water content might lead to lower sensitivity of the trees to meteorological factors. R. pseudoacacia was more sensitive to changes of soil water content.
AssuntosTranspiração Vegetal/fisiologia , Quercus/fisiologia , Robinia/fisiologia , China , Solo/química , Árvores , Água/análise
This study investigated the feasibility of using hyperspectral imaging (HSI) to characterize the diffusion of acid and water within food structures during gastric digestion. Two different sweet potatoes (steamed and fried) and egg white gel (pH5 and pH9 EWGs) structures were exposed to in vitro gastric digestion before scanning by HSI. Afterward, the moisture or acid present in the digested sample was analyzed for calibration purposes. Calibration models were subsequently built using partial least-squares (PLS). The PLS models indicated that the full-wavelength spectral range (550-1700 nm) had a good ability to predict the spatial distribution of acid (Rcal2 > 0.82) and moisture (Rcal2 > 0.88). The spatiotemporal distributions of moisture and acid were mapped across the digested food, and they were shown to depend on the food composition and structure. The kinetic data revealed that the acid and moisture uptakes are governed by Fickian diffusion or by both diffusion and erosion-controlled mechanisms.


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text: Achievement Details Withdrawal Symptoms - 20 points As a team, collected Power from all 4 Power Taps within one wave of Horde Be the first to Add a Guide for this achievement! Login or Register to Add a Guide for this Achievement! Recent Achievers Stallion83 Tuesday 10th of September 2019 11:15:15 PM


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There are many factors that go into determining your car insurance rates. Some, like your age and gender, are out of your control. Others, like where you live and your marital status, are somewhat in your control. Finally, there are several factors that affect your auto insurance rates, like the insurance company you choose to work with, your driving record, your credit score, the kind of car you drive, and the amount of coverage you choose, that are completely within your control.
Non-owner car insurance is just what it sounds like. It's insurance that covers the driver instead of the car. That is, if you don't own a car, but frequently drive a friend's car, rental cars, work cars, or use a car-sharing service, non-owner insurance covers your liability in the event of an accident. It can cover your liability for medical costs and property damage. In some states, non-owner car insurance can also help you regain your license after it's been suspended. It can also lower car insurance rates if you buy a car later since there won't be an uninsured period on your record. |
Next, take a good hard look at your driving record and credit report, and clean both up if they're not in good shape. Moving violations, citations for driving under the influence, accidents, and a low credit score all tell car insurance companies you're at higher risk of being in an accident, making a claim, and costing them money. Some car insurance companies will give you a discount if you can prove you're a safe driver by installing a tracking device in your car, which can lead to lower rates over time (however, if the tracker shows you routinely drive in an unsafe manner, your rates might go up). A few years without a citation or an accident, as well as a steadily improving credit score, can help you save on car insurance.
Insurance companies cannot raise premiums instantaneously to reflect incurred losses. That's because rate changes—in most states—must be submitted and reviewed by the state's department of insurance before they can go into effect. As a result, rate changes that are brought on by a loss-causing event—such as a hurricane—may take some time to go into effect.
USAA maintains a strong financial standing and ranked the highest of our top picks with Consumer Reports and J.D. Power. This means you won't have to worry about settling up financially with the company, and you'll likely have a decent time maneuvering through its claims process. If you or anyone in your immediate family is an active or retired service member, you should definitely give USAA a call and get a quote.
There isn't a definitive answer to the question, "which company is the cheapest?" Some companies are cheaper than others, plain and simple, but individual details of your driving profile can affect which companies offer you the cheapest rates. For example, while Nationwide wasn't the cheapest for a driver with a clean record, it was the relatively cheap for a driver following an at-fault accident. The best way to find cheap car insurance is to compare as many companies as possible using your driving profile.
Travelers: Travelers is the second-best car insurance company. Travelers' customers report that they appreciate the customer service Travelers provides, and the company gets high marks for how it resolves its customers' claims. Travelers' lost a few points from customers who didn't feel like they got good value from the company, and Travelers' average annual rates are a bit higher than average among all companies on this list.
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There is a case to be made for getting just comprehensive and not collision insurance, even if your car is not valuable. Comprehensive covers you for a lot more perils than does collision--including, most importantly, against theft. Regardless of the value of your car, having it stolen is a major inconvenience. Even if your car is worth only $2,000 at the time of the theft, and your insurer gives you $1,500, that sum would go a long way in buying yourself a new vehicle. As we discuss in more detail below, comprehensive insurance generally costs no more than $200 per year, so a $1,500 reimbursement would make the coverage valuable.
If you live in an area with unusual state regulations or heightened risk of weather-related claims, shopping car insurance options will be vital. Not every car insurance company offers policies in every state, which can make pricing less competitive. If you live in storm-prone states like Louisiana or Florida, you might find it harder to get a competitive rate.
American Family: American Family car insurance is the lowest-ranked company on our list. While a fair number of customers said filing a claim with American Family was easy, that was about the only positive thing they had to say, with the company faring poorly on measures of customer service, communication about claim status, value, and claim resolution.
A car insurance quote from The General® requires no personal information (your name, phone number, street address, etc.) to provide an accurate car insurance quote. Once you receive your anonymous auto insurance quote, there is absolutely no commitment on your part. You can save your auto insurance quote online at any point during the process and return to it at your leisure.
If you insure more than one vehicle, you may be able to stack your uninsured/underinsured motorist coverage. For example, let's say you cover two vehicles on the same policy and each vehicle has a standard $25,000 in uninsured motorist protection. If you have stacked coverage and sustain injuries in a crash caused by an uninsured driver, you'll be able to draw from both payouts for a combined total of $50,000 in coverage.


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Posted in: Denny Tamaki, critic of U.S. bases, elected Okinawa governor See in context
With his win, the Chinese are happy about it. The former Governor went to China for a talk with the Chinese politician. Hope they won't regret if U.S. bases leaves Japan. China can enter freely in Okinawa. As most regrets the leaving of U.S. bases in the Phil. And now claim the islets as theirs including EEZ
-3 ( +10 / -13 )
I saw the incident on TV, I have to say I wasn't convinced that it was a racist gesture. Looked…
Posted in: Man arrested on suspicion of racism at Manchester derby
Of course the Taliban will deny it, it was probably a mistaken attack and admitting to it would be a…
Posted in: Body of slain doctor returns home to Japan from Afghanistan
Really want to hear who is responsible for this horrific senseless act. RIP.
Posted in: Body of slain doctor returns home to Japan from Afghanistan
Let's enjoy liberals trying to play with stats. This is the truth: But getting down to that point was a…
Posted in: Democrats zoom in on Trump impeachment charges this week


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BSc (Hons) BioscienceUniversity Centre Peterborough
Program description
The main aims of this degree are to provide you with a high level of knowledge and understanding of the core biological processes and principles as applied to bioscience. This course will also equip you with the skills that enable you to acquire, interpret and analyse relevant scientific information with a critical understanding of the appropriate contexts for their use. Core to this degree is the development of your understanding of the industry applications of bioscience and the development of work based practical skills and knowledge to underpin your academic studies. With this philosophy in mind the BSc (Hons) Bioscience has been designed to deliver an academic qualification that will fully prepare you for employment in today's diverse bioscience industry.
About this institute
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Totoro & Hydrangea Mini Art Crystal Jigsaw Puzzle
Studio Ghibli
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Official Studio Ghibli licensed item, imported from Japan.
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This is a collectible item, recommended for ages 15+


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Posted By: Imright, 11/11/2019 12:36:01 PM
Amazon announced it would be opening a new grocery store in California as the company looks to expand into the grocery market beyond its purchase of Whole Foods. "Amazon is opening a grocery store in Woodland Hills in 2020," a representative for the company told CNET on Monday. The company said the store would differ from Whole Foods but did not expand on its prices or offerings.The grocery store is being heralded as "Amazon's first grocery store," according to job listings. In 2017, Amazon purchased Whole Foods for $13 billion


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Home - Unitarian Universalist Service Committee
Language: English
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Early Signs of Autism in a Baby
Posted on Author Blaine FaulkPosted in Articles, BlogLeave a Reply
What are the signs of autism in a baby, and how to become aware of them? Becoming a parent has its challenges and fears. You never want to believe that your precious bundle has a problem, but when it comes to autism, catching it early, ideally by the age of 18 months, makes a huge…


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This is Chiswick
A Chis-wiki of features about Chiswick's people and places.
What makes Chiswick special? Its architecture? Its open spaces? We are lucky to have Chiswick House & Gardens, Hogarth's house, Gunnersbury Park, Gunnersbury Triangle nature reserve, Dukes Meadows, and the ever-changing landscape of the river.
Its history? Its thriving culture and strong sense of community? We have some very popular annual events such as the Oxford & Cambridge boat race, the Bedford Park Festival, Chiswick Book Festival, Chiswick House Dog Show, and now Lovebox and Citadel festivals which attract thousands of people.
Its people? There are many actors, musicians and writers who live in Chiswick, including a few very famous ones, who contribute to its cultural life, and all sorts of other people doing all manner of interesting things.


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Entertain Peaceful leafy Stadium View in your apartment!!
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