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Stressing the need for women empowerment, Prime Minister Sheikh Hasina today said Bangladesh women are doing a great job for the development of the country.
Women and Children Affairs Ministry organised the programme at the Osmani Memorial Auditorium in Dhaka, marking the Begum Rokeya Day with State Minister for Women and Children Affairs Meher Afroz Chumki in the chair.
Two eminent women personalities— Aroma Dutta and Begum Noorjahan were conferred to the prestigious Rokeya Padak this year for their 'contribution to the society for the development of the country.'
In her address, renewing her call to protect woman rights in every sphere of the public life, the prime said her government made notable success in reducing child marriages in the country.
She also said women rights will have to be realised by themselves as no one will come up with those in men ruled society. Mentioning that half of the country's population is women, the Prime Minister said their development is a must for the country's development. "If women don't advance in education and other sectors, the society will never build up properly. All have to understand that there is no chance of neglecting them," she said.
Aroma Dutta and Begum Nur Jahan also expressed their feelings at the programme. The government introduced the award in 1995 after the name of Begum Rokeya, the pioneer of awakening women's rights in the subcontinent.


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Mao Bao, Inc. is engaged in the processing, manufacture, distribution, and import and export trading of cleansing products. The company's products include laundry detergent, household cleansing products, spontex series, personal care products and other products. The laundry detergent products include washing condense, collar detergent, soften agents and others. The household cleansing products are used for glass, kitchen, bathroom and floor cleaning. The spontex series include gloves, mops, towels and vegetable cloths. The personal care products include shower creams, hand cleansers, shampoos and others. Mao Bao was founded on December 6, 1978 and is headquartered in Taipei, Taiwan.
|Current Board Membership
|Chieh Cheng Huang, Independent Director
|Mao Bao, Inc.
|Liang Su, Independent Director
|Mao Bao, Inc.
|Revenue -0.56%
|Net Income -33.53%
|Earnings Per Share -33.53%
|Capital Spending +31.96%
|Gross Margin +42.66%
|Cash Flow +195.18%

subset: commoncrawl


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Headquarters Salon & Spa , Anchorage , Alaska 99502 (AK )
Headquarters Salon & Spa in Anchorage
Local Business
Anchorage Alaska 99502 AK
Headquarters Salon & Spa Reviews: Take Our Quick 5 Question Survey And Leave A Comment
About Headquarters Salon & Spa
Headquarters Salon & Spa is mostly involved in Local Business. Headquarters Salon & Spa operates in Alaska. This business establishment is involved in Local Business as well as other possible related aspects and functions of Local Business. In Anchorage Headquarters Salon & Spa maintains its local business operations and could perform other local business operations outside of Anchorage Alaska 99502 in additional functions related to Local Business.
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Job Positions Available In Anchorage, AK
Scheduling is flexible during business hours, Monday through Friday 8:00am to 5:00pm, with some evenings required. $23.00/hour + Potential for year-end bonus.View Job and Apply
Experience working with licensing programs or other programs requiring screening qualifications of individuals and businesses; To explain these to others;View Job and Apply
Because this position requires travel for official business, the selectee will be required to apply for a charge card within 30 calendar days of appointment.View Job and Apply
Selection process, please make your request to the examiner, selecting official or local manager of Human Resources. This job has an exam requirement.View Job and Apply
Technicians, nurses, clinical leads – the face of our business – are the ones providing outstanding patient care in more than 1 million procedures every year.View Job and Apply


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High School Fleet Smartphone Game Delayed to Spring 2019
posted on by Karen Ressler
The website for the iOS and Android game based on the High School Fleet anime announced on Friday that the game is now slated for Spring 2019. The game was originally slated for a 2018 release, but the staff explained it delayed the release to improve the game's quality.
The game's Twitter account also posted a commercial:
【ゲームCM公開】— 【公式】ハイスクール・フリート 艦隊バトルでピンチ! (@hai_furi_app) December 21, 2018
事前登録も宜しくお願いいたします!#はいふり #艦隊バトルでピンチ pic.twitter.com/gh2DrJJs5A
The original 12-episode television anime series premiered in Japan in April 2016. Aniplex of America licensed the series, and the show streamed on Crunchyroll, Funimation, and Daisuki. An original video anime (OVA) shipped on Blu-ray Disc in May 2017. An anime film has been greenlit
Aniplex of America describes the story of the television series:
Living by the sea, protecting the sea, and journeying across the sea...They're the BLUE MERMAIDS! Because of a shift in tectonic plates around 100 years ago, Japan lost much of its area to submergence. To preserve Japan's territories, Coastal Cities sprung up, one after the other. Eventually, they became Marine Cities, and along with the expansion of sea-lanes to connect them, the need arose for a sizable staff to protect the seas. At the same time, maritime jobs were becoming more popular among women. Hence, the BLUE MERMAIDS, tasked with keeping the seas safe, had become every female student's dream job. This is when childhood friends Akeno Misaki and Moeka China enroll in a marine high school in Yokosuka, along with other girls who share their goal of "BECOMING A BLUE MERMAID."
AAS/AIS and Kanari Abe launched the main manga adaptation in Kadokawa's Comic Alive magazine in October 2015, and the manga is ongoing. Kadokawa published the manga's fourth volume on July 23. AAS/AIS and Tsuchii (Girls und Panzer: Little Army, Girls und Panzer: Little Army II) launched the High School Fleet: Maidens of Loreley (High School Fleet: Loreley no Otome-tachi) spinoff manga manga in the same magazine in June 2016, and ended it in August 2017.


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It is American whiskey, without question that is synonymous with the cocktail; all of the classic mixes were based around the American spirit. The Manhattan, the Old-Fashioned, the Sazerac - all classics based on the rustic spirit from America. The whiskey possesses the wonderful high notes and spice because of the prolific use of virgin oak in the maturation. Rye whiskey is one of the original American spirits, the basis of many of the classic cocktails we still enjoy today, including the Manhattan. In America, rye whiskey must have a mash bill consisting of at least 51% rye amongst other grains.
1776 Straight Rye Whiskey comes from the James E. Pepper brand, a name that has long been known in the American whiskey world, though not as much recently as it was in the early 1900s. Happily, the… More info
Another terrific rye whiskey from James E. Pepper's range, this time bottled at 92 proof (which is 46% ABV for those of us here on this side of the ocean). Makes an excellent Old Fashioned cocktail,… More info
The 1776 whiskey range from James E. Pepper grows with the introduction of the 1776 Straight Rye Whiskey - Barrel Proof. It's the same whiskey as the fantastic 1776 Straight Rye Whiskey, though… More info
Straight rye whiskey here from the Bower Hill range, which takes its name from the location of the first bloodshed of the 1794 Whiskey Rebellion - the Battle of Bower Hill. More info
Has there ever been a moment when you've been sipping Bulleit Bourbon and wanted it to have even more rye? Well you're in luck, they now have their own Rye whiskey! Bulleit Rye is made with a… More info
A rye whiskey from Cedar Ridge, a distillery which usually chooses to showcase corn. This expression is made up of 70% rye, 25% corn and 5% malted barley, and after distillation the liquid is aged in… More info
Bottled in Bond and named for Colonel Edmund Haynes Taylor - a staunch supporter of the 1897 Bottled in Bond Act, this is a fabulous rye whiskey which is aged for four years before bottling. More info
Straight rye from the Dad's Hat range, made with locally harvested grain from Pennsylvania and aged for at least 3 years before bottling at 47.5% ABV. Boasting all those yummy spices you're… More info
This is a straight rye whiskey from Ezra Brooks, a long-running American whiskey brand. Excellent for enjoying in a Manhattan. More info
A collaborative rye whiskey from the excellent FEW Spirits in Evanston, Illinois, and legendary psychedelic rock band The Flaming Lips! Their Brainville Rye (named after a song on The Flaming Lips'… More info
Gun Fighter Rye from Golden Moon begins its maturation in new American oak casks before being finished in French oak American fortified wine casks, which is rather intriguing indeed... More info
Here we have Heaven's Door Straight Rye, created by Bob Dylan (yes, that exact Bob Dylan you're thinking of) and Marc Bushala (founder of Angel's Envy Bourbon) in a church in Tennessee.… More info
Thanks to Tuthilltown Spirits, we can once again enjoy whiskey from New York, with this one in particular harking back to the pre-prohibition age of whiskey distillation in and around The Big… More info
New York is home to many amazing things, like the Empire State Building, more pizza than you could eat in five lifetimes, and Tuthilltown Spirits - makers of the excellent Manhattan Rye! Harking back… More info
The Jack Daniel's range grew in 2017 with the introduction of their Tennessee Rye whiskey - the first whiskey from the distillery with a mash-bill that different from the classic Tennessee Whiskey… More info
One of three batches of rye whiskey from James E. Pepper independently bottled by That Boutique-y Whisky Company, each one finished in a different type of cask. As pointed out by James E. Pepper's… More info
The third batch of rye whiskey from James E. Pepper which has been bottled by That Boutique-y Whisky Company. Just like batch 1 and 2, this whiskey has enjoyed a finishing period in different types… More info
Great value rye whiskey from Jim Beam. It's a favourite of whisky bible author Jim Murray, and won best rye aged under 10 years in 2010, scoring 88 points. The label received a bit of a redesign in… More info
It's here! Knob Creek's Rye Whiskey is finally here! Unveiled in the US in 2012, Knob Creek Rye has made its way to our fair shores, bringing with it a flavour profile that strikes a wonderful… More info
Made with 100% rye, which Koval source locally, this is a single barrel release aged in American oak from Minnesota. More info
A straight rye whiskey from the Michter's Distillery in Louisville, Kentucky. Every bottle of their excellent rye comes from a single barrel, highlighting the quality of their craft. A top choice for… More info
Single barrel 10 year old straight rye whiskey from Michter's distillery out in Louisville, Kentucky. Wonderful stuff, packed with spicy rye goodness and well balanced notes of toffee and vanilla. More info
Minor Case Straight Rye comes to us via Limestone Branch, and takes its name from Minor Case Beam - yes, relative of that famous whiskey-makin' Beam family. An interesting rye which has enjoyed a… More info
A fantastic rye whiskey produced in Kentucky, the Pikesville brand dates back to 1895 in Maryland. This 6 year old 110 proof was released in 2015, receiving a Double Gold at the San Francisco World… More info
New York Distilling Company's rye whiskey has long been anticipated by cocktail enthusiasts around the world. Well wait no longer, mixologists, Ragtime Rye is ready to enjoy! Using a mashbill of 72%… More info


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Upcoming Events
From music festivals, car races, history, heritage, food and everything in between. Here's your guide.
Events for August 17, 2019
All Day
Northeast Tennessee Water Media Innovations Art Show
NE TN Water Media Innovations Art Show August 4 – August 25 – during regular park hours Reception and Demonstration: Saturday, August 10 th Water Media Demonstration 10 am – 1 pm Reception 2 pm – 4 pm This new art event is sponsored by the Watauga Valley Art League and focuses on watercolor andFind out more »
Crockett Days
Join us on August 16-18, 2019 for our annual Crockett Days celebration. Visit the Crockett Homestead, shop for 18th century wares, and attend excellent programming about frontier life. We have many special surprises in store, 18th century style. Camping, firewood and hospitality is provided on the beautiful Nolichucky River for reenactors. https://www.facebook.com/events/2110958279121512/Find out more »
High Knob Outdoor Festival
For more details go online to https://highknoboutdoorfest.wixsite.com/highknoboutdoorfest?fbclid=IwAR1PPRcr4iuCeIVddPIwUznWRzulHmE3u4UpUlFrkvs4A7xrRL28Jt04hSU.Find out more »
Sycamore Shoals State Historic Park: Summer Ranger Program
Sycamore Shoals State Historic Park History at Home Summer Ranger Programs – August 2019 Seasonal Interpretive Rangers – Ian Anderson & Laura Ellis Carter Mansion and Sabine Hill Historic House Tours August dates are listed throughout the calendar- Pre-registration is required Go to www.tnstateparks.com/parks/sycamore-shoals Choose "Upcoming Events" in the left hand menu to registerFind out more »
Abingdon: Barter Theatre Presents: Church Basement Ladies
Church Basement Ladies Written by Jim Stowell and Jessica Zuehlke, Music and Lyrics by Drew Jansen Based on the book Growing up Lutheran by Janet Letnes Martin & Suzann Nelson "I enjoyed watching my companions rock with laughter and revel in the show." The ladies of the local church fortify their flock with love, wisdomFind out more »
Abingdon: Barter Theatre Presents: Shrek: The Musical
Shrek: The Musical Music by Jeanine Tesori, Book and Lyrics by David Lindsay-Abaire Follow the adventures of Shrek, his sidekick Donkey, Princess Fiona, Lord Farquaad and every fairytale adventure you know and love in this Tony Award-winning musical based on the blockbuster DreamWorks Animation film. A little green ogre grows up to be a big,Find out more »
Abingdon: Barter Theatre Presents: Exit Laughing
Exit Laughing A comedy by Paul Elliott "The Golden Girls" meets "Steel Magnolias." For this group of genteel southern ladies, the biggest highlight in their lives for the past 30 years has been their weekly bridge night out with the "girls." What do you do when one of your foursome, whose caustic wit and loveFind out more »
Abingdon: Barter Theatre Presents: La Cage Aux Folles
La Cage Aux Folles Music and Lyrics by Jerry Herman, Book by Harvey Fierstein Based on the French movie of the same name, which later spawned the American movie "The Birdcage," this Jerry Herman musical remains one of the all-time biggest hits worldwide. This multi-Tony Award-wining musical tells the story of a middle-aged show businessFind out more »
12:00 am
Backpacking 101 Class
Must Register! Have you been thinking about getting into backpacking but aren't sure where to start? We have you covered! Our Backpacking 101 Class introduces you to all you need to know to be able to hit the trail for multiple days so that you can extend your adventure, access remote locations and experience naturalFind out more »
Doe River Snorkeling Excursion
Escape the summer heat and join Park Ranger Philip Hylen for a snorkeling excursion on the doe river! This event will begin at 3:00PM starting at the Roan Mountain State Park Visitor Center. From there, participants will motorcade to the Peg Leg section of the Doe River. Participation costs $10.00 a person and is limitedFind out more »
Rocky Fork Water Quality Sampling
Hosted by Overmountain Trout Unlimited and Rocky Fork State Park Grab sampling for our Embrace-A-Stream project, monitoring the water quality of Rocky Fork Creek. We will meet in the main parking lot and head out from there. Anyone is welcome to attend and learn how to take samplesFind out more »
MAHC: Yellow Mountain
Mid-Appalachian Hiking Club Hike: 7-8 miles Rating: Moderate Drive: 39 miles ($5) The hike will start from Roaring Fork Road, catching the AT to the side trail which leads to the open bald. Weather permitting, we should enjoy 360 - degree views from our destination. If we catch this area in full bloom with allFind out more »
5 & Dime Group Run
Looking for a group to run with on those longer runs? What is a 5 & Dime? 5 miles or 10 miles, runners choice, we provide the routes for both! We meet at the store, hang our keys, pick our route, and go for run! Make sure to click going/interested to get a weekly reminder!Find out more »
Tot Triathlon
Ages: 3-6Registration: Early registration - July 15 - Aug. 15 $10/person Late registration - $15/person NO Day of Registration Register in person at: Memorial Park Community Center, 510 Bert St. It's never too early to offer opportunities for a healthy lifestyle and this new event is designed to do just that. Preschoolers will begin byFind out more »
7:00 am
Watauga Lake Triathlon
https://www.facebook.com/Watauga-Lake-Triathlon-113517595334680/?__tn__=%2Cd%2CP-R&eid=ARAAZgRuGwYAp4ecSYCTwU_rRqwVCw5FHmTF2vosG6wJ7mh7XvsEGqac7ZU0sPNCr_nn-bqxZNIygU8RFind out more »
8:00 am
Jonesborough: Movies on Main
Movies on Main kicks off Summer Sunset Saturdays in Tennessee's Oldest Town as folks enjoy Jonesborough's Historic District on Saturday evenings during the summer. On select Saturdays, Movies on Main will take place in front of the Lollipop Shop beginning with the popular Candy Bingo at 8 p.m. followed by a family-friendly movie at 8:30Find out more »
9:00 am
Greeneville: Farmers Market
The Greeneville Farmer's Market will return to the Doak House Museum in May of 2019. Visit the Market Saturdays from 9 a.m. to 1 p.m, and Wednesdays in July and August, for the best local veggies, baked goods, hand-crafts, artisan foods, and much more. Special events all summer long! Watch the newsletter for updates.Find out more »
10:00 am
Elizabethton: Watuaga Valley Fife and Drum Corps
Watauga Valley Fife and Drum Corps 1st and 3rd Saturdays 10am - Noon The Watauga Valley Fife & Drum Corps performs at historical events, dedications, and parades. Musical experience is welcome but not necessary, just come with a willingness to learn.Find out more »
Storytelling Live!
Storytelling Live! The 18th season of ISC's signature concert series is underway! Storytelling Live! is a six-month showcase of the nation's top talent. Each week, we invite a different "teller in residence" to Jonesborough for five days of live storytelling performances. Designed to complement the National Storytelling Festival, Storytelling Live! features talented tellers who specialize inFind out more »
2:00 pm
Community Jam
Calling all musicians! Join us at the Birthplace of Country Music Museum for a full-on community jam session! The Birthplace of Country Music Museum is now hosting a monthly gathering of local musicians for a full-on community jam session! The jam events have been organized to provide a space for local musicians to gather andFind out more »
Storytelling Live!
Storytelling Live! The 18th season of ISC's signature concert series is underway! Storytelling Live! is a six-month showcase of the nation's top talent. Each week, we invite a different "teller in residence" to Jonesborough for five days of live storytelling performances. Designed to complement the National Storytelling Festival, Storytelling Live! features talented tellers who specialize inFind out more »
5:00 pm
Jonesborough: Classic Car Cruise-In
Jonesborough's Classic Car Cruise-In at the Chuckey Depot Museum at Jonesborough is set to take place again on Saturday, August 17th from 5 to 8 p.m. and will continue each third Saturday through October. Have the chance to view rare vintage cars, enjoy great food, tour the Chuckey Depot Museum and listen to live music.Find out more »
7:30 pm
Bass Pro Shops NRA Night Race
The campfires, the laughs, the racing, the music, the sights, smells and the check off your bucket list. Fierce clashes and heart-stopping finishes are hallmarks of the most popular race in NASCAR, and if history's any indication The Bass Pro Shops NRA Night Race is guaranteed to entertain guests with yet another epic battle insideFind out more »


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Some prominent local roads have featured in a state government regional road improvement funding announcement.
In total the state government will fix over 1000km state-wide with the Karoonda highway listed for shoulder sealing works.
As well the state government will also deliver federal government's 87.5 million dollar upgrade of the strut highway corridor between Renmark & Gawler.
Minister for Transport, Infrastructure and Local Government Stephan Knoll said "The Marshall Government is building better regional roads that will create jobs, improve road safety and help save lives on our country roads,"
Mr Knoll also took aim at the former Labor government saying "For 16 long years Labor neglected SA regions and the Marshall Government is investing in our regions to create jobs, build better roads and clear the backlog left by Labor."


text:
- Project plans
- Project activities
- Legislation and standards
- Industry context
Last edited 24 Jul 2017
Company acquisitions in construction
Acquisitions, together with mergers, are major strategic initiatives that can be undertaken by organisations wishing to grow, or change their structure. Also known as takeovers, acquisitions involve one company taking a controlling interest in another. Mergers on the other hand involve combining companies to form one single company, where the two original companies cease to exist and a third company is created.
In the construction industry, acquisitions are commonly used as means of accelerating growth, reducing the effects of the construction boom and bust cycle, entering into new markets, and spreading risk. Acquisitions can also allow expansion into the global construction market by increasing the depth of international expertise and the business portfolio.
It is generally expected that acquiring a company will result in a bigger organisation. Akintoye and Skitmore examined the effect of firm size on returns over the period 1980-1987. They concluded that larger firms have persistently higher rates of return that are attributable to greater managerial efficiency.
There are several different types of acquisition:
- Friendly takeover: An acquisition that is approved by management and shareholders.
- Hostile takeover: An acquisition that does not have the approval of management but is pursued by the bidding company anyway.
- Reverse takeover: Where a private company acquires a public company.
- Backflip takeover: Where the acquiring company turns itself into a subsidiary of the purchased company, for example, where a larger company takes over a smaller company with an established brand identity.
Issues that an organisation might consider when assessing a possible acquisition might include; whether it will create economic gains; the type of business available; the sort of diversification required; their growth strategy; the compatibility of the two organisations and so on.
Acquisitions can have a range of impacts on an organisation, from 'soft' issues such as the culture of the organisation, to 'hard' issues such as integrating IT facilities and accounts.
Financing for an acquisition might include:
- Bank loan or an issue of bonds. Leveraged buyouts are acquisitions that are financed through debt which is often moved down onto the balance sheet of the company being acquired.
- Loan note alternatives: Allowing shareholders to take part or all of their consideration in loan notes rather than cash, which can be more attractive in terms of taxation.
- All share deals: The bidding company issues new shares to the shareholders of the acquired company, rather than paying money.
- All-cash deals: A simple acquisition for an amount of money per share.
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38 résultat.s à cette recherche
Emmanuel Macron's openness toward Russia is testing the patience of NATO allies
Dans les médiasFrançois Heisbourg (cité), CNN, 3 décembre 2019
Military Exercises: The Russian Way
Book chapterIsabelle Facon,
in The Russian Military in Contemporary Perspective (US Army War College, SSI), September 2019
In a Trump world, diplomacy is being conducted in French (again)
Dans les médiasBruno Tertrais (interview), POLITICO, 7 septembre 2019
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The European Centre of Excellence for Countering Hybrid Threats, September 2, 2019
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Pouvoirs, July 21, 2019
Trump's Trap: Europe, Iran, and the Future of the Nuclear Deal
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In Russia, France's Macron Tries His Next Charm Offensive On 'Cher Vladimir'
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I make music of different genres and have several projects:
SHRIK3 - EBM/Industrial/Dark-Electro/Synthwave
Bandcamp Link This is my general artist name. But to make things less messy, I decided to put only electronic tracks under this title.
- Synthesized Joy (Full album, released on bandcamp)
- Off Sync (EP single, released on Xiami.com)
- A Neon Dream (Synthwave EP album, not released yet)
Vom Verborgenen - Black Metal/Melodic Death/Post Black
- Vom verborgenen (EP album, 3 songs + 1 bonus, released on bandcamp)
- the stars were going out (EP album, released on bandcamp)
Return 0; - Melodic Death/Metalcore
This is my college band. No album released yet. Instrumental versions are available on Xiami.com.
Experimental stuff.. Various Genres.
I also make post rock and even funky stuff. Though I don't have a seperate title for them. All above can be found on Xiami.com under the name of SHRIK3


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Date Fitness Singles Near Me - Browsing Lesbian Fitness Women
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Anyone from Innocean here? I'm curious what their creative team structure is and how the creative team works along side the live events/production team.
Just read about Springer at innocean being put on leave for sexual harassment. Are we approaching a #timesup moment in advertising?
Hyundais official response distances itself from Innocean. I guess they don't care to keep the females of Hyundai safe. They're ok with a potential predator and his enablers in their offices.Shameful
Anyone know much about Innocean Huntington Beach in regard to creative leadership? And do they do anything other than Hyundai?


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PurCBD+ uses the most reliable suppliers for phytocannabinoid oil for humans. Everything from the ingredients to the bottle and dropper are human grade. The owners and staff of Innovet use PurCBD on ourselves and our own pets. Our goal has always been to make the product equal or better in every way to its human-use counterparts. However, due to the extra liability involved in marketing the product for human use, officially we are required to say that it's only for pets.
When the founders of Love CBD, Phil and Dan Culbertson, saw the success of CBD oil in the USA, they saw an opportunity for launching a CBD business in the UK. This family-run business from Newmarket, Suffolk, realised that many of the CBD oils available in the UK at that time (in 2014) were of substandard quality and decided to become CBD connoisseurs and put together blends with what they considered to be the best hemp strains out there.
BioBloom Hemp believes that their range of drops, 4%, 6% and 8% extracts, are all you need. Not only if you're getting started with CBD oil, but also if you're looking to take a high-strength concentrate. How can you accomplish a 40% effect (the strongest available) with a 4% concentrate? You would just need to take more of it; so, instead of taking 1 drop of a 40% CBD, you take 10 drops of BioBloom's 4% CBD drops. The CBD content is the same, but BioBloom says that this equivalence comes with an added benefit: you get more of the full-spectrum cannabinoid profile. In other words, by taking more of the oil, you take in more cannabinoids, thus enhancing the effect of the main active ingredient.
One of the most expensive tinctures on the list, Pet Releaf's full-spectrum CBD oil is carried in organic coconut (MCT) oil, and has a very light taste. You'll be able to choose between 3 concentrations for both cats and dogs — just make sure to read their product pages closely so you know how much CBD is in the bottle. The numbers on their label refer to all the cannabinoids present in the tincture, not just CBD.
With an increasing number of options out there, choosing the right CBD oil brand may be confusing. Unfortunately, CBD oil is currently not regulated by the FDA, hence not all brands are organic, and not all include the same amount of or full-spectrum benefits. When choosing a CBD oil brand, look for organic, non-GMO, independently tested, full-spectrum hemp products.
Vaping CBD oil is one of the preferred methods to ease side effects of diseases, illnesses, and ailments including Leukemia, all types of Cancers, stress, anxiety, joint pain, seizures, inflammation, sleep apnea, high blood pressure and the list goes on. There are many benefits to CBD vape oil. However, the effectiveness of CBD helping with these conditions (and many others) hasn't yet been scientifically proven, and many trials are still in pre-clinical stage.
Unlike NSAIDs, many doctors believe that acetaminophen does not increase your risk of stomach or heart issues. They are often recommended to those whose stomach cannot tolerate NSAIDs well. However, acetaminophen is far from ideal for your body. The long-term use and overuse of acetaminophen have been linked to liver problems and acute liver failure.
Always striving to source full-spectrum products (rich in other synergetic cannabinoids like CBDa), when Love Hemp launched, it focused on bringing fine organic hemp oils from the EU, particularly from the Czech Republic, into the UK market. For a while, they partnered with Endoca, who allowed them to source organic hemp material from mainland Europe which they would white label under their premium brand in the UK. Later on, when cannabis legislation changed in the USA, they tested Colorado-grown organic hemp from a company called Folium and, because of their guaranteed high quality, they decided this would become the principal source of their CBD hemp oils. All Love Hemp products are THC free (below the 0.2% allowed by UK law), non-addictive, non-toxic and third-party tested for purity.
What attracts Silvija so much about hemp as a skincare agent is the many active "skin foods" it contains: Omega 3, 6 and 9, bioflavonoids, terpenes, phytochemicals, phenolic compounds, and amino acids. At Hemptouch, all hemp materials used come from their own family hemp farm in Slovenia and have been cold pressed in situ. The unrefined, full-spectrum CBD oil serves as the main ingredient in their skincare line. When we checked each label of their CBD creams and ointments, we found that no parabens, SLS/SLES, artificial colours or mineral oils have been used in any of their formulations. There are no corners cut at Hemptouch and their ethical prices turn it into a staple brand to keep in your cupboard or handbag. Visit the Hemptouch collection.
CBD oil vape juice like nicotine requires you to increase dosage to be able to experience the intended effect. Cannabis vape juice has different unique flavors and each person perceives the taste differently. Just like CBD vape oil effects, the taste varies from earthly to grassy and sometimes people refer to flowers when they describe their taste experience.
Thank you for the information. A friend of mine recently gave me a small bottle of Cloud 9 hemp, lizard king. Said she was told it is good for COPD. Can not find anything that backs up that statement, You article also said that Cloud 9 is a brand that uses Propylene and the effects on the lungs when vaporizing has not been determined but OK to use externally. Is all this correct?
We work hard to provide you with the best selection of CBD Vape Oil products from the best brands out there. You'll find some of the best deals on CBD E-Juices and E-Liquids available right here. We carry brands like Americana Uncut, For the People, Hemp Bombs, Pure CBD, Hempzilla, and many more. Fast Shipping to all 50 US States. When you need CBD E-Liquid for your vaporizer, remember Pure CBD Vapors – Your Trusted CBD Source!


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Category of Documents
..... 129 of CGST/SGST Act - HELD THAT:- The reasons shown in Exts.P8 and P11 notices, that are impugned in this writ petition, are not sufficient for the purposes of detaining the goods in terms of Section 129 of the CGST/SGST Act - the 1st respondent is directed to forthwith release the goods and the vehicle to the petitioner on the petitioner producing a copy of this judgment before the said responde .....
Full Text of the Document
..... ay bill, it was shown as ₹ 25.66. It is also the case of the detaining authority that the commodity in question was undervalued by the vendor by offering excessive discounts to the purchaser. 2. I have heard the learned counsel appearing for the petitioner and also the learned Government Pleader appearing for the respondents. 3. On a consideration of the facts and circumstances of the case a .....
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INTRODUCTION: There is evidence that mesenchymal stem cells (MSCs) could trans-differentiate into the liver cells in vitro and in vivo and thus may be used as an unfailing source for stem cell therapy of liver disease. Combination of MSCs (with or without their differentiation in vitro) and minimally invasive procedures as laparoscopy or Natural Orifice Transluminal Endoscopic Surgery (NOTES) represents a chance for many patients waiting for liver transplantation in vain. METHODS: Over 30 millions of autologous MSCs at passage 3 were transplanted via the portal vein in an eight months old miniature pig. The deposition of transplanted cells in liver parenchyma was evaluated histologically and the trans-differential potential of CM-DiI labeled cells was assessed by expression of pig albumin using immunofluorescence. RESULTS: Three weeks after transplantation we detected the labeled cells (solitary, small clusters) in all 10 samples (2 samples from each lobe) but no diffuse distribution in the samples. The localization of CM-DiI+ cells was predominantly observed around the portal triads. We also detected the localization of albumin signal in CM-DiI labeled cells. CONCLUSION: The study results showed that the autologous MSCs (without additional hepatic differentiation in vitro) transplantation through the portal vein led to successful infiltration of intact miniature pig liver parenchyma with detectable in vivo trans-differentiation. NOTES as well as other newly developed surgical approaches in combination with cell therapy seem to be very promising for the treatment of hepatic diseases in near future.
AssuntosTransplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Veia Porta , Animais , Cirurgia Endoscópica por Orifício Natural , Suínos , Porco Miniatura , Transplante Autólogo
The aim of this research was to study the migration of multipotent mesenchymal stromal cells (MMSC) in old laboratory animals under physiological conditions and after liver resection. Different routes of administration were used: to the caudal vein, intraperitoneal, hepatic artery, portal vein. Studies have shown the ability of the old organism to respond to changes in the directed migration of MMSCs in the tissues that have undergone the greatest damage, which may be due to the production of connective tissue of the damaged organ chemoattractant. In contrast intraperitoneal, other delivery methods MMSC: tail vein, v. portae, a. hepatica, are more effective.
AssuntosMovimento Celular , Fígado , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Animais , Animais de Laboratório , Fígado/cirurgia , Transplante de Células-Tronco Mesenquimais/métodos , Células-Tronco Mesenquimais/citologia
Mesenchymal stem cells (MSCs) intrinsically possess unique features that not only help in their migration towards the tumor-rich environment but they also secrete versatile types of secretomes to induce nerve regeneration and analgesic effects at inflammatory sites. As a matter of course, engineering MSCs to enhance their intrinsic abilities is growing in interest in the oncology and regenerative field. However, the concern of possible tumorigenesis of genetically modified MSCs prompted the development of non-viral transfected MSCs armed with nanotechnology for more effective cancer and regenerative treatment. Despite the fact that a large number of successful studies have expanded our current knowledge in tumor-specific targeting, targeting damaged brain site remains enigmatic due to the presence of a blood-brain barrier (BBB). A BBB is a barrier that separates blood from brain, but MSCs with intrinsic features of transmigration across the BBB can efficiently deliver desired drugs to target sites. Importantly, MSCs, when mediated by nanoparticles, can further enhance tumor tropism and can regenerate the damaged neurons in the central nervous system through the promotion of axon growth. This review highlights the homing and nerve regenerative abilities of MSCs in order to provide a better understanding of potential cell therapeutic applications of non-genetically engineered MSCs with the aid of nanotechnology.
AssuntosNeoplasias Encefálicas/terapia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/citologia , Nanotecnologia/métodos , Regeneração Nervosa , Animais , Neoplasias Encefálicas/irrigação sanguínea , Neoplasias Encefálicas/patologia , Humanos , Tropismo
Multiple sclerosis (MS) is a chronic autoimmune inflammatory disease of the central nervous system (CNS). In attempt to identify an appropriate treatment for improving the neurological symptoms and remyelination process, autologous and allogenic transplantation of mesenchymal stem cells (MSCs) have been introduced as an effective therapeutic strategy in MS. MSCs are a heterogeneous subset of pluripotent non-hematopoietic stromal cells that are isolated from bone marrow, adipose tissue, placenta and other sources. MSCs have considerable therapeutic effects due to their ability in differentiation, migration, immune-modulation and neuroregeneration. To date, numerous experimental and clinical studies demonstrated that MSCs therapy improves the CNS repair and modulates functional neurological symptoms. Here, we provided an overview of the current knowledge about the clinical applications of MSCs in MS. Furthermore, the major challenges and risks of MSCs therapy in MS patients have been elucidated.
AssuntosTransplante de Células-Tronco Mesenquimais/métodos , Esclerose Múltipla/terapia , Animais , Humanos , Transplante de Células-Tronco Mesenquimais/efeitos adversos , Esclerose Múltipla/patologia
This article serves to provide an overview of molecular and surgical interventions to minimize the progression of posttraumatic arthritis following high-energy intra-articular fractures. The roles of cartilage and the microcellular environment are discussed, as well as the response of the joint and cartilage to injury. Molecular therapies, such as glucocorticoids, mesenchymal stem cells, and bisphosphonates, are presented as potential treatments to prevent progression to posttraumatic arthritis. High-energy intra-articular fractures of the elbow, hip, knee, and ankle are discussed, with emphasis on restoring anatomic alignment, articular reduction, and stability of the joint.
AssuntosCartilagem Articular/lesões , Fraturas Intra-Articulares/complicações , Osteoartrite/terapia , Artroplastia , Difosfonatos/uso terapêutico , Progressão da Doença , Glucocorticoides/uso terapêutico , Humanos , Ácido Hialurônico/uso terapêutico , Transplante de Células-Tronco Mesenquimais , Osteoartrite/etiologia
Nowadays, ionizing radiations have numerous applications, especially in medicine for diagnosis and therapy. Pharmacological radioprotection aims at increasing detoxification of free radicals. Radiomitigation aims at improving survival and proliferation of damaged cells. Both strategies are essential research area, as non-contained radiation can lead to harmful effects. Some advances allowing the comprehension of normal tissue injury mechanisms, and the discovery of related predictive biomarkers, have led to developing several highly promising radioprotector or radiomitigator drugs. Next to these drugs, a growing interest does exist for biotherapy in this field, including gene therapy and cell therapy through mesenchymal stem cells. In this review article, we provide an overview of the management of radiation damages to healthy tissues via gene or cell therapy in the context of radiotherapy. The early management aims at preventing the occurrence of these damages before exposure or just after exposure. The late management offers promises in the reversion of constituted late damages following irradiation.
AssuntosTerapia Baseada em Transplante de Células e Tecidos/métodos , Terapia Genética/métodos , Lesões por Radiação/prevenção & controle , Proteção Radiológica/métodos , Amifostina/uso terapêutico , Animais , Antioxidantes/uso terapêutico , Ensaios Clínicos como Assunto , Fracionamento da Dose de Radiação , Edição de Genes , Vetores Genéticos/uso terapêutico , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/uso terapêutico , Transplante de Células-Tronco Hematopoéticas , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/genética , Peptídeos e Proteínas de Sinalização Intercelular/uso terapêutico , Transplante de Células-Tronco Mesenquimais , Camundongos , Oxirredutases/genética , Oxirredutases/uso terapêutico , Lesões por Radiação/etiologia , Lesões por Radiação/terapia , Lesões Experimentais por Radiação/prevenção & controle , Lesões Experimentais por Radiação/terapia , Protetores contra Radiação/farmacologia , Protetores contra Radiação/uso terapêutico , Proteínas Recombinantes/genética , Proteínas Recombinantes/uso terapêutico , Fatores de Tempo
OBJECTIVE: To observe the repair effect of bone marrow mesenchymal stem cells (BMSCs) combined with basic fibroblast growth factor (bFGF) on spinal cord injury in rats and explore its mechanism. METHODS: SD rat BMSCs were obtained by serum culture technique. Eighty healthy 6-week-old male SD rats(weight about 240 g) were randomly divided into 4 groups with 20 each. The sham operation group underwent simple laminectomy without damaging spinal cord and was kept in the same condition as the other 3 groups. The other 3 groups underwent left T9 spinal cord hemisection to establish spinal cord injury model. After 9 days of modeling the local transplantation was performed. The Control group was implanted with gelatin sponge containing normal saline. The BMSCs transplantation group was implanted with gelatin sponge containing BMSCs. The bFGF+BMSCs transplantation group was implanted with gelatin sponge containing bFGF+BMSCs. After 4 and 8 weeks, the expression of NF-200 and GFAP in injured spinal cord tissue was analyzed by Western blotting and the recovery of hind limb function was evaluated by Basso Beattie Bresnahan(BBB) motor function score scale. RESULTS: The BBB scores of BMSCs transplantation group and bFGF+BMSCs transplantation group were better than control group at 4 and 8 weeks after operation (P<0.05) and there was significant difference between bFGF+BMSCs transplantation group and BMSCs transplantation group (P<0.05). After 4 and 8 weeks postoperatively, NF-200 expression was minimal in control group and only a small amount was expressed in BMSCs transplantation group while in bFGF+BMSCs transplantation group NF-200 was highly expressed(P<0.05). GFAP expression was high in control group, middle in BMSCs transplantation group and low in bFGF BMSCs transplantation group(P<0.05). There was significant difference between bFGF+BMSCs transplantation group, BMSCs transplantation group and control group(P<0.05). CONCLUSIONS: The combined transplantation of BMSCs and bFGF can repair the spinal cord injury in rats. The mechanism may be related to the decrease of GFAP expression and the increase of NF-200 expression.
AssuntosTransplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Traumatismos da Medula Espinal , Animais , Células da Medula Óssea , Fator 2 de Crescimento de Fibroblastos , Masculino , Ratos , Ratos Sprague-Dawley , Medula Espinal
Exosomes are extracellular vesicles with the size of 40-100â¯nm in diameter and a density of 1.13-1.19â¯g/mL, containing proteins, mRNAs, miRNAs, and DNAs. Exosomes change the recipient cells biochemical features through biomolecules delivery and play a role in cellular communication. These vesicles are produced from body fluids and different cell types like mesenchymal stem cells (MSCs). Evidence suggests that mesenchymal stem cells-derived exosome (MSC-EXO) exhibit functions similar to MSCs with low immunogenicity and no tumorization. MSCs can also be isolated from a variety of sources including human umbilical cord (HUC). Because of the non-invasive collection method, higher proliferation and lower immunogenicity, HUCMSC-EXO has been frequently used in regenerative medicine and various diseases treatment compared to the other MSC-EXO resources. This review aimed to investigate the applications of HUCMSC-EXO in different diseases.
AssuntosDoença , Exossomos/fisiologia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/citologia , Medicina Regenerativa , Cordão Umbilical/citologia , Animais , Humanos
Although intravenous injection is the most convenient and feasible approach for mesenchymal stem cells (MSCs) delivery, the proportion of donor stem cells in the target myocardium after transplantation is small. It is believed that TCM enhances the effect of stem cell therapy by improving the hostile microenvironment and promoting the migration and survival of stem cells. Guanxin Danshen (GXDS) formulation is one of the main prescriptions for clinical treatment of ischemic heart diseases in China. The purpose of this study was to evaluate the effects of GXDS formulation administration combined with MSCs transplantation on cardiac function improvement, apoptosis, angiogenesis and survival of transplanted cells in an acute model of acute myocardial infarction (MI). After being labeled with GFP, MSCs were transplanted via intravenous injection. Meanwhile, GXDS dripping pills were given by intragastric administration for 4â¯weeks from 2â¯days before MI. Echocardiography showed moderate improvement in cardiac function after administration of GXDS formulation or intravenous transplantation of MSCs. However, GXDS formulation combined with MSCs transplantation significantly improved cardiac function after MI. The myocardial infarct size in rats treated with MSCs was similar to that in rats treated with GXDS formulation. However, GXDS formulation combined with MSCs transplantation significantly reduced infarction area. In addition, GXDS formulation combined with MSCs transplantation not only decreased cell apoptosis according to the TUNEL staining, but also enhanced angiogenesis in the peri-infarction and infarction area. Interestingly, the use of GXDS formulation increased the number of injected MSCs in the infarct area. Furthermore, GXDS formulation combined with MSCs transplantation increased SDF-1 levels in the infarcted area, but did not affect the expression of YAP. Our study provided a more feasible and accessible strategy to enhance the migration of stem cells after intravenous injection by oral administration of GXDS formulation. The combination of GXDS formulation and stem cell therapy has practical significance and application prospects in the treatment of ischemic cardiomyopathy such as MI.
AssuntosApoptose/efeitos dos fármacos , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Transplante de Células-Tronco Mesenquimais/métodos , Células-Tronco Mesenquimais/citologia , Infarto do Miocárdio/terapia , Neovascularização Patológica/prevenção & controle , Animais , Células Cultivadas , Terapia Combinada , Sobrevivência de Enxerto , Masculino , Infarto do Miocárdio/patologia , Ratos
The treatment of neural deficiency after cerebral infarction is challenging, with limited therapeutic options. The transplantation of mesenchymal stem cells (MSCs) to the ischemic penumbra is a potential therapeutic approach. In the present study, a cerebral infarction model was generated by performing middle cerebral artery occlusion (MCAO) in SD rats. The expression of CXCR4 increased, and the number of MSCs migrating to the peri-infarct area was higher in rats transplanted with preconditioned MSCs than in rats transplanted with untreated MSCs. The rate of apoptosis, as evaluated by TUNEL staining and immunoblotting assays, was reduced in rats receiving preconditioned MSCs. A significant amelioration of neural regeneration and improved neurological function were observed in rats injected with preconditioned MSCs compared with those injected with untreated MSCs. However, the application of an siRNA targeting CXCL12 significantly inhibited the protective role of preconditioned MSCs against apoptosis and promoted the migration of MSCs to the ischemic area, leading to impaired neuronal regeneration and limited recovery of neuronal function. Hypoxic preconditioning of MSCs prior to transplantation suppressed apoptosis and increased their migration abilities, leading to the promotion of neuronal regeneration and improvement in neural function after transplantation. This preconditioning strategy may be considered as a potential approach for the modification of MSCs prior to cell transplantation therapy in patients with cerebral infarction. SIGNIFICANCE OF THE STUDY: We found that hypoxic preconditioning of MSCs improved their ability to promote neuronal regeneration and the recovery of neuronal function. Moreover, we showed that CXCR4 inhibited apoptosis, improved cell homing, and promoted neuronal differentiation, without influencing angiogenesis. Our study provides a relatively safe preconditioning method for potential use for cell transplantation therapy in ischemic cerebral infarction. The results presented here will facilitate the development of novel strategies and techniques to improve the tolerance and migration ability of transplanted cells for the treatment of cerebral infarction sequelae.
AssuntosInfarto Cerebral/metabolismo , Quimiocina CXCL12/metabolismo , Modelos Animais de Doenças , Hipóxia , Células-Tronco Mesenquimais/metabolismo , Receptores CXCR4/metabolismo , Animais , Apoptose/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular , Infarto Cerebral/terapia , Quimiocina CXCL12/antagonistas & inibidores , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/efeitos dos fármacos , Neurônios , RNA Interferente Pequeno/farmacologia , Ratos , Ratos Sprague-Dawley , Receptores CXCR4/antagonistas & inibidores , Transdução de Sinais/efeitos dos fármacos
Exotic animal orthopedics has not incorporated the most recent progress made in small animal surgery or human medicine. Although minimally invasive osteosynthesis has been incorporated as a routinely used alternative in small animals, its use in exotic animals is still in its infancy. This article compliments the reviews of orthopedics in small mammals, birds, and reptiles in the previous issue. It reviews relevant recent studies performed in laboratory animals about new orthopedic materials and techniques showing potential to become incorporated into the routine orthopedic treatment of exotic animals in the coming years.
AssuntosAnimais Exóticos , Fixação Interna de Fraturas/veterinária , Equipamentos Ortopédicos/veterinária , Ortopedia/veterinária , Animais , Aves , Pinos Ortopédicos/veterinária , Placas Ósseas/veterinária , Parafusos Ósseos/veterinária , Transplante Ósseo/veterinária , Fixação Interna de Fraturas/tendências , Fixação Intramedular de Fraturas/tendências , Fixação Intramedular de Fraturas/veterinária , Humanos , Mamíferos , Transplante de Células-Tronco Mesenquimais/veterinária , Equipamentos Ortopédicos/tendências , Ortopedia/métodos , Próteses e Implantes/veterinária , Répteis
Diabetes Mellitus (D.M.) is a disease with a high and increasing prevalence. The Insulin- producing Cells (IPCs) derived from the Wharton's jelly of human umbilical cord transplantation was thought to be the most promising strategy for treating Diabetes. This study aimed to evaluate IPCs immune modulatory changes occurred after transplanted through two different routes and the effect of these changes on their therapeutic efficiency in relation to transplantation microenvironment. Insulin Producing Cells was induced to differentiate from human Umbilical Cord-Mesenchymal Stem Cells and characterized by morphology under phase contrast inverted microscope and staining of secretory granules by DTZ (diphenylthiocarbonazone) stain, then therapeutic effect was evaluated both in vitro and in vivo through glucose challenge test and hyperglycemia correction in STZ (streptozotocin)- induced diabetic rats. Immune-modulatory changes evaluated by cell- mediated lysis assay and Syber green quantification of immune inflammatory cytokines (IFN- ï§, TGF- ß and IL-10) gene expression by real-time PCR. We observed that in spite of the weak immunogenicity of induced IPCs derived from HUC-MSCs in vitro, but when transplanted in vivo especially through the intra portal vein they could induce an immune response when interact with the disease microenvironment resulting in different degree of inflammatory response. Therefore, the relationship between disease microenvironment and immune alteration should be examined before transplantation therapy.
AssuntosDiabetes Mellitus Experimental/imunologia , Insulinas , Transplante de Células-Tronco Mesenquimais , Geleia de Wharton/citologia , Animais , Diferenciação Celular , Citocinas/imunologia , Diabetes Mellitus Experimental/terapia , Humanos , Células-Tronco Mesenquimais/citologia , Ratos , Cordão Umbilical/citologia
Mesenchymal stem cells (MSCs) are able to exert immunomodulatory and anti-inflammatory actions. Thanks to these properties, MSCs may be a promising alternative approach for the treatment of inflammatory disease. Important cytokines involved in inflammation are those included in the IL-1 family. Interleukin-37 (IL-37) is one of the member able to suppress both innate and adaptive immunity. Recently, it was found that MSCs and their derivatives can modulate IL-37, and MSCs expressing IL-37 seem to have an enhanced therapeutic efficacy.
AssuntosInterleucina-1/fisiologia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/citologia , Citocinas , Humanos , Inflamação
Reconstruction of mandibular defects is a challenging issue in maxillofacial surgery. Success of the treatment is defined by the process of reparative regeneration of bone tissue, which is often accompanied with wound infection, failure of microcirculation and tissue hypoxia. Aim of our project was to demonstrate the advantage of osteoplastic material and bone marrow derived mesenchymal stem cells combination in regeneration of lower jaw bone defect. For this reason, mandibular bone defect was created in laboratory rats. Experimental treatment of animals was performed in three different ways: group 1. - Bone defect was regenerated without any treatment, group 2. - Bone defect was filled with osteoplastic material (BIO-OSS), group 3. - Bone defect was filled with BIO-OSS, which was previously infiltrated by bone marrow mesenchymal stem cells. Animals were under observation for 6 months. During this period bone regeneration was comparatively analyzed in these tree experimental groups. Observational, histochemical and x-ray research methods were used in this study. Our results suggest that use of osteoplastic material and mesenchymal stem cells combination can increase formation of red bone marrow, which is normally slow under natural condition. Therefore, we can conclude that application of osteoplastic material with bone marrow derived mesenchymal stem cells can decrease time of bone defect osteointegration and define the quality of regenerated bone tissue.
AssuntosMateriais Biocompatíveis/uso terapêutico , Regeneração Óssea , Substitutos Ósseos , Mandíbula/cirurgia , Transplante de Células-Tronco Mesenquimais/métodos , Células-Tronco Mesenquimais , Animais , Osso e Ossos/patologia , Osso e Ossos/cirurgia , Mandíbula/patologia , Ratos , Engenharia Tecidual
OBJECTIVES: The bone tissue engineering primarily focuses on three-dimensional co-culture systems, which physical and biological properties resemble the cell matrix of actual tissues. The complex dialogue between bone-forming and endothelial cells (ECs) in a tissue-engineered construct will directly regulate angiogenesis and bone regeneration. The purpose of this study was to investigate whether co-culture between osteogenic and angiogenic cells derived by bone mesenchymal stem cells (MSCs) could affect cell activities and new bone formation. MATERIALS AND METHODS: Mesenchymal stem cells were dually induced to differentiate into osteogenic cells (OMSCs) and ECs; both cell types were co-cultured at different ratios to investigate their effects and underlying mechanisms through ELISA, RT-qPCR and MTT assays. The selected cell mixture was transplanted onto a nano-hydroxyapatite/polyurethane (n-HA/PU) scaffold to form a cell-scaffold construct that was implanted in the rat femoral condyles. Histology and micro-CT were examined for further verification. RESULTS: ELISA and gene expression studies revealed that co-cultured OMSCs/ECs (0.5/1.5) significantly elevated the transcription levels of osteogenic genes such as ALP, Col-I and OCN, as well as transcription factors Msx2, Runx2 and Osterix; it also upregulated angiogenic factors of vascular endothelial growth factor (VEGF) and CD31 when compared with cells cultured alone or in other ratios. The optimized OMSCs/ECs group had more abundant calcium phosphate crystal deposition, further facilitated their bone formation in vivo. CONCLUSIONS: The OMSCs/ECs-scaffold constructs at an optimal cell ratio (0.5/1.5) achieved enhanced osteogenic and angiogenic factor expression and biomineralization, which resulted in more effective bone formation.
AssuntosMateriais Biomiméticos/química , Regeneração Óssea/fisiologia , Osso e Ossos/fisiologia , Células-Tronco Mesenquimais/citologia , Tecidos Suporte/química , Animais , Osso e Ossos/diagnóstico por imagem , Osso e Ossos/patologia , Diferenciação Celular , Células Cultivadas , Técnicas de Cocultura , Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo , Durapatita/química , Células Endoteliais/citologia , Células Endoteliais/metabolismo , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/metabolismo , Osteogênese , Ratos , Ratos Sprague-Dawley , Engenharia Tecidual , Fatores de Transcrição/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo
Blindness and vision loss contribute to irreversible retinal degeneration, and cellular therapy for retinal cell replacement has the potential to treat individuals who have lost light sensitive photoreceptors in the retina. Retinal cells are well characterized in function, and are a subject of interest in cellular replacement therapy of photoreceptors and the retinal pigment epithelium. However, retinal cell transplantation is limited by various factors, including the choice of potential stem cell source that can show variability in plasticity as well as host tissue integration. Dental pulp is one such source that contains an abundance of stem cells. In this study we used dental pulp-derived mesenchymal stem cells (DPSCs) to mitigate sodium iodate (NaIO3) insult in a rat model of retinal degeneration. Sprague-Dawley rats were first given an intravitreal injection of 3â¯×â¯105 DPSCs as well as a single systemic administration of NaIO3 (40â¯mg/kg). Electroretinography (ERG) was performed for the next two months and was followed-up by histological analysis. The ERG recordings showed protection of DPSC-treated retinas within 4â¯weeks, which was statistically significant (* Pâ¯≤â¯.05) compared to the control. Retinal thickness of the control was also found to be thinner (*** Pâ¯≤â¯.001). The DPSCs were found integrated in the photoreceptor layer through immunohistochemical staining. Our findings showed that DPSCs have the potential to moderate retinal degeneration. In conclusion, DPSCs are a potential source of stem cells in the field of eye stem cell therapy due to its protective effects against retinal degeneration.
AssuntosIodatos/toxicidade , Transplante de Células-Tronco Mesenquimais , Degeneração Retiniana/terapia , Epitélio Pigmentado da Retina/efeitos dos fármacos , Animais , Apoptose/efeitos dos fármacos , Polpa Dentária/citologia , Modelos Animais de Doenças , Eletrorretinografia , Masculino , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/metabolismo , Células Fotorreceptoras/citologia , Ratos , Ratos Sprague-Dawley , Degeneração Retiniana/etiologia , Epitélio Pigmentado da Retina/patologia
AIMS: The inflammation modulation effects of mesenchymal stromal cell-derived exosomes (MSC-EXO) are well established. We aimed to explore the mechanism behind the inflammatory responses of numerous exosomal cargo molecules that have been neglected in molecular biology research, and to develop an exosomal cargo delivery system that can exert a stronger therapeutic effect on myocardial ischemia-reperfusion (I/R) injury. MAIN METHODS: Computational approaches were used to identify key exosomal miRNAs and their downstream mRNAs that are expressed in the inflammatory response. Direct interactions between miRNA-181a and the c-Fos mRNA complex were confirmed by luciferase reporter assay. MSC-EXO carrying miRNA-181a-overexpressing lentiviruses were intramyocardially injected into a mouse model of myocardial I/R injury. I/R progression was evaluated through echocardiography and immunofluorescence microscopy. KEY FINDINGS: miRNA-181a provided substantial coverage against a host of immune-related genes through the miRNA-mRNA network. miRNA-181a delivery by MSC-EXO combined the immune-suppressing effect of miRNA-181a and the cell targeting capability of MSC-EXO to exert a stronger therapeutic effect on myocardium I/R injury. SIGNIFICANCE: We showed the potential of MSC-EXO as a tool for the specific delivery of small RNAs in vivo. This study shed new light on the potential application of miRNA-181a-overexpressing MSC-EXO as a therapeutic strategy for myocardial I/R injury.
AssuntosCélulas-Tronco Mesenquimais/metabolismo , MicroRNAs/sangue , Traumatismo por Reperfusão Miocárdica/metabolismo , Animais , Movimento Celular/fisiologia , Proliferação de Células/fisiologia , Transplante de Células-Tronco de Sangue do Cordão Umbilical/métodos , Exossomos , Humanos , Inflamação/terapia , Masculino , Transplante de Células-Tronco Mesenquimais/métodos , Células-Tronco Mesenquimais/citologia , Camundongos , Camundongos Endogâmicos C57BL , MicroRNAs/genética , MicroRNAs/metabolismo , Traumatismo por Reperfusão Miocárdica/genética , Traumatismo por Reperfusão Miocárdica/patologia , Traumatismo por Reperfusão/genética , Traumatismo por Reperfusão/metabolismo
AIM: Mesenchymal stem cells (MSCs) have several properties that may support their use as an early treatment option for osteoarthritis (OA). This study investigated the role of multiple injections of allogeneic bone marrow-derived stem cells (BMSCs) to alleviate the progression of osteoarthritic changes in the various structures of the mature rabbit knee in an anterior cruciate ligament (ACL)-deficient OA model. MATERIALS AND METHODS: Two months after bilateral section of the ACL of Japanese white rabbits aged nine months or more, either phosphate buffered saline (PBS) or 1 x 106 MSCs were injected into the knee joint in single or three consecutive doses. After two months, the articular cartilage and meniscus were assessed macroscopically, histologically, and immunohistochemically using collagen I and II. RESULTS: Within the PBS injection (control group), typical progressive degenerative changes were revealed in the various knee structures. In the single MSC injection (single group), osteoarthritic changes were attenuated, but still appeared, especially in the medial compartments involving fibrillation of the articular cartilage, osteophyte formation in the medial plateau, and longitudinal tear of the meniscus. In the multiple-injections group, the smoothness and texture of the articular cartilage and meniscus were improved. Histologically, absence or reduction in matrix staining and cellularity were noticeable in the control and single-injection groups, respectively, in contrast to the multiple-injections group, which showed good intensity of matrix staining and chondrocyte distribution in the various cartilage zones. Osteoarthritis Research Society International (OARSI) scoring showed significantly better results in the multiple-injections group than in the other groups. Immunohistochemically, collagen I existed superficially in the medial femoral condyle in the single group, while collagen II was more evident in the multiple-injections group than the single-injection group. CONCLUSION: A single injection of MSCs was not enough to restore the condition of osteoarthritic joints. This is in contrast to multiple injections of MSCs, which had the ability to replace lost cells, as well as reducing inflammation. Cite this article: Bone Joint J 2019;101-B:824-831.
AssuntosTransplante de Células-Tronco Mesenquimais/métodos , Osteoartrite do Joelho/terapia , Animais , Ligamento Cruzado Anterior/cirurgia , Injeções Intra-Articulares , Osteoartrite do Joelho/etiologia , Osteoartrite do Joelho/patologia , Coelhos , Transplante Homólogo , Resultado do Tratamento
Bronchopulmonary dysplasia (BPD) is one of the most common chronic lung diseases in neonates especially in preterm infants. It is also the main reason leading to a poor prognosis. The prognosis of the neonates with BPD is unsatisfactory with current treatment strategies. Recent clinical trails have found that mesenchymal stem cell (MSC) transplantation might be effective and promising for treatment of BPD in neonates. This article outlines the characteristics of MSC and the potential mechanisms of MSC transplantation for BPD in vivo, and the safety and feasibility of MSC transplantation in BPD neonates, as well as the challenges in clinical trials on MSC transplantation for treatment of BPD.


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Scott Morrison urges Australian businesses in London to adopt 'strategic patience' over Brexit – The Sydney Morning Herald
The Sydney Morning Herald
Scott Morrison urges Australian businesses in London to adopt 'strategic patience' over Brexit
The Sydney Morning Herald
London: Treasurer Scott Morrison is urging the Australian business community in London to adopt "strategic patience" as they wait to find out whether Britain will still enjoy special passporting rights into the EU after Brexit. The issue is key for ...
More lessons on fintech to come for Scott Morrison
Morrison urges open Brexit arrangements
Morrison Pushing For Australia-UK FTA
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