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Is it worth it to buy a Trak-Ball?
Jstick replied to 7800 Guy's topic in Atari 2600Which of the 2 trackball variants is recommended for the 2600?
Atari in the News - Cool BBC Article!
Jstick replied to hizzy's topic in Atari 2600From the article, it would seem that this is more of a general algorithm problem then one of programming or VCS peculiarities. Basically trying to extract a logical mathematical pattern from a fixed data table.
Oh absolutely. I must have spent several years now heavily curating a personal ROM collection, and I'm close to being done. Problem is, even just taking the cream of the crop across all the systems I'm interested in, it still ends up being multiple lifetimes worth of games. It's really just too much, and starts to feel like a buffet vs a nice, intimate restaurant in terms of experience. These days, spending time with my 2600 is just about the only thing that gives me those true gaming vibes I miss (which would sound insane to my 10 year old self, considering the 40 years worth of gaming history available at my fingertips.). An arcade cabinet or pinball table will do it too of course, but those are unfortunately not very practical for me right now 🙂
This I cannot argue with! 😀
Emulation is definitely convenient; not sure I would say the shift towards it is absolutely thrilling though. More of a sad inevitability, I would say. Around '98 at its genesis emulation was very exciting, being able to play Neo Geo and then CPS-1 and 2 games (and N64 with a voodoo card was awesome). These days having thousands of ROMs from every system ever made is just kind of... tiring. In the same way that having unlimited music streaming and access to pretty much all commercial music just makes everything feel blah. I see it most in the younger generation now, they treat games and other media as disposable, very transitory experiences, always moving on to the next thing. I don't have time left to enjoy all the media available at my fingertips, it's a dream to think that anyone could. All I need are a few good experiences that I can sit with and digest slowly; that have some impact; that become "events" in my life rather than just forgettable background noise. There is something to be said for having a small curated library of physical books or games or albums or movies on a shelf. Things that you love and cherish, that you revisit, that you put the effort into exploring simply because they are the few things that you own, that you picked. That vinyl album that you bought when you had you had your first girlfriend, the game cart your dad gave you and stayed up to play with you, that favourite book you have read so many times the pages are worn from your fingers. The artwork, the lyrics sheet, the shape, form, smell and feel of it... older game hardware and electronics have some interesting and beautiful design. Digital media has no history, no tactile quality, no emotion imbued into it. Just cold bits on a storage device, infinitely copyable and deletable. Amazing from a preservation and access point of view (would have been mind-blowing to my younger self). But in reality, the amount of choice and lack of tangibility are a bit of a curse. Ultimately, attachment to physical objects may simply be a side-effect of the particular brand of commercialism instilled into us Gen-X folks, but I can't deny having genuine emotions towards to the relics of my youth. They will forever be tied to people and places and feelings that marked my life, and I like being able to hold those things in my hand. My children will know nothing of this, as they will be plugged into a 24/7 stream of endless digital content emanating from uniform featureless devices. In the end the title of this post is actually apt: "RetroPie Killed It For Me". For me what emulation killed was the special feeling of getting a new game and the excitement of playing it for weeks or months; when you have access to everything, nothing is special any longer. (Horrible first-world problem, isn't it?)
Stella 6.0 releasedSomebody has to create all the of content that these people are now consuming in ever-increasing amounts; and if you are any kind of content creator (Software developer, 3D Modeler, Web Designer, Video Editor, Music Composer, Digital Artist, etc. etc.) you will definitely want at LEAST 2 monitors.
Thanks, I had just assumed the differences in brightness between pictures was due to lighting conditions. My box is light gray in fact (though not as light as the bottom picture), so I'm guessing it is NTSC after all. Really, the thing that initially confused me was the addition of Italian, Chinese and German.
10% Off Homebrew games, Free Game with $200 Purchase in AtariAge Store
Jstick replied to Albert's topic in Atari 2600On the 2600 side, Ladybug Super Cobra Mappy Draconian Space Rocks Chetiry would be a good start.
The sheer awesomeness that is PETE ROSE BASEBALL
Jstick replied to JayAre's topic in Atari 2600I wonder if changing the home run frequency might be as simple as changing a byte value in the code.
We need a better Pole Position!
Jstick replied to Greg2600's topic in Atari 2600I imagine Out Run should probably be inserted in there somewhere
Giant Label Variation PHOTO Website. Check it out!
Jstick replied to Philflound's topic in Atari 2600I wonder if it would help to make a page where you list all your currently missing items. Then it would be easier to see what remains for people who are interested in contributing. Although I'm not sure if that's more work than just finding the stuff yourself.
Giant Label Variation PHOTO Website. Check it out!
Jstick replied to Philflound's topic in Atari 2600A very commendable goal! At this point in time I think we have game ROMs pretty well covered from a preservation point of view; it's the related paper-based ephemera that still remains an issue (boxes, manuals, inserts, etc.). My long-term fantasy pet project is to create a searchable game database with every cart, box and manual viewable as 3D models. Good source material would be essential to this endeavour.
Stella 6 on the R77: the eagle is landing
Jstick replied to DirtyHairy's topic in Atari 2600I've had a MicroSD card die that way before: couldn't be formatted or written to by any OS, device or software; but could be read no issue. Frozen in time forever.
This is the PAL box: And this is the NTSC:
Can't tell if sealed game is PAL or NTSC (Kangaroo)
Jstick posted a topic in Atari 2600I have a sealed copy of Kangaroo (1988) which I always assumed was PAL as it has 6 languages on the back. However I just noticed on AtariMania that the North American release from that year appears to use the same international box for some reason. Game was purchased from someone in the US. Any way to discern the region without opening?


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D - 95 mm
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r min. - 1,1 mm
Weight - 0,683 Kg
Basic dynamic load rating (C) - 71 kN
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(Grease) Lubrication Speed - 6 700 r/min
(Oil) Lubrication Speed - 7 900 r/min
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This website uses cookies to remember your settings, statistics and target ads. This information is shared with third parties. | Close
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"Found hotel easy to access, and close to railway station. Staff friendly and helpful. " More
All 80 soundproofed rooms feature LED TVs with satellite channels, and you can enjoy sofa beds, safes and hairdryers. Fridges and round-the-clock room service make in-room dining convenient. Pillowtop beds and premium bedding welcome you to a good night's rest, and other nice touches include rainfall showerheads and designer toiletries. Additionally, pillow menus, down comforters and phones are available.
The restaurant, Ristorante Ottantanove, serves Italian cuisine and is open for breakfast, lunch and dinner. Ottantanove Bar-Lounge is the hotel's own lobby lounge, serving drinks along with dinner and light fare. Other amenities include free WiFi in public areas and coffee/tea in a common area. Buffet breakfast is available on weekdays between 7 AM and 10 AM and on weekends between 7:30 AM and 10:30 AM. There's also self parking, along with the convenience of an airport shuttle.
Attractions within a 30-minute walk of this 3.5-star Munich City Centre hotel include Church of Our Lady (Frauenkirche) and Rathaus-Glockenspiel.
26 Jun. 2019 by Hotel Reservations
Dear Guest, Thank you for taking the time to review your stay with us. We appreciate your excellent rating and were pleased to read you were satisfied with the hotel location and property. We are sure our entire hotel staff enjoyed catering to your needs as much as you enjoyed your stay. We do look forward to having you again as a guest in our hotel! Sincerely
28 Mar. 2018 by Aimilia Zorba, Reservation Department
Dear Guest. Thank you for taking the time to review your stay with us. We are so glad to hear that you enjoyed our hospitality. We look forward to welcoming you to VI VADI HOTEL BAYER 89 again in the near future.
There's a restaurant on site. You can enjoy a drink at the bar/lounge. WiFi is free in public spaces. An airport shuttle (available 24 hours) is available for a fee. Tour or ticket assistance, vending machine, and multilingual staff are also featured at the business-friendly Vi Vadi Hotel Bayer 89. The hotel can provide concierge services and limo/town car service. Limited parking is available for a fee and is offered on a first-come, first-served basis. This 3.5-star Munich hotel is smoke free.
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
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Vi Vadi Hotel Bayer 89 offers 80 forms of air-conditioned accommodation with a safe and designer toiletries. This individually decorated accommodation includes sofa beds. Pillowtop beds feature Egyptian cotton sheets, down comforters and premium bedding. A pillow menu is available. 40-inch LED televisions come with satellite channels. Bathrooms include a shower with a rainfall showerhead, complimentary toiletries and a hairdryer. Guests can surf the web using complimentary wireless Internet access. Business-friendly amenities include desks and telephones. Hypo-allergenic bedding and an iron/ironing board can be requested. Housekeeping is provided on a daily basis.
For a fee, guests can enjoy buffet breakfast on weekdays from 7 AM to 10 AM and at the weekend from 7:30 AM to 10:30 AM.
Ristorante Ottantanove – This restaurant specialises in Italian cuisine and serves breakfast, lunch and dinner.
Ottantanove Bar-Lounge – This lobby lounge specialises in international cuisine and serves dinner and light fare. Open daily.
24-hour room service is available.
Special check-in instructions:
24-hour airport shuttle service is available upon request. Fees may apply. Contact the property in advance to make arrangements.
Airport transport is offered on request 24 hours a day. Fees may apply. Please make arrangements in advance by contacting the property.
Charges for extra guests may apply and vary according to property policy.
A cash deposit, credit card or debit card for incidental charges and government-issued photo identification are required upon check-in.
Special requests are subject to availability at the time of check-in. Special requests can't be guaranteed and may incur additional charges.
The fees and deposits listed below will be charged at the time of service, check-in, or check-out.
This may not be a comprehensive list. Fees and deposits are subject to change and might not include tax.


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You tell us what you need, when. There are some standard spec items to iron out, but it is not unusual for this to be a very quick conversation or two. If a more detailed and formalized spec, or an RFP is required for your project, we can accommodate.
Deposit Half of Project Fee
For new clients, we typically work out to receive at least half of the project fee up-front. We accept many forms of payment, and will work with you to find the best method. Once the deposit is settled, we begin work immediately. Existing clients with solid track records do not have to pay half up-front, but can execute new projects with a simple email or phone call and settle when we wrap.
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We work very quickly, and will ramp our team's size to match the flow of work. We allow for two rounds of revisions, but we rarely even get one – the discovery process eliminates guesswork. Typically, a few preliminary pages/efforts will be completed for review and assurance that we are on track – then we go at it, hard.
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I'm thrilled to have launched my website and am incredibly grateful to Marty for making it happen. With focus on details, he constructed the site from scratch and allowed the vision in my head to become a reality. His down to earth personality made him a joy to work with, but more importantly, his wealth of knowledge about search engine optimization strategically allows my site to be found more quickly by my target market. I am grateful too for the brilliant ideas Marty shared about marketing and visibility. These invaluable tools will allow me to mover forward effectively and successfully. I strongly recommend Marty for those of you seeking the same.K.J. - website build client
Marty was exceptional to work with. His knowledge of web development, design and SEO was superb. Even though our work is very technical in nature, he was still able to provide a lot of input into documents. His own work was always completed in a timely fashion and he really required no management time at all.A.W. - client of 2.5 years
Marty's technical strengths and industry knowledge were exactly what I needed when I decided it was time for a "real" website. Marty and I brainstormed concepts on what I wanted the site to do and how I wanted to convey my message – then he made it happen! He managed my ideas, content and complete lack of "techno" savy with expertise, and actually made me enjoy the work involved in developing the site. It was a pleasure to collaborate with Marty on this very creative, yet highly technical project. And thanks to Marty's SEO muscle, I now have a web site that functions (and delivers) as my #1 marketing tool. He gets my highest recommendation!J.L. - longterm client (over 5 years)
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I reached level 9 then the automatic machine guns werent shooting properly anymore. Other than that, I'm amazed how polished this game for a Game Jam. Great work
Viewing post in Mitosis jam comments
Yeah, that's the only potentially game breaking bug I'm aware of. Can't believe I missed that... Thanks for playing!


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How AI is Changing the Business Landscape
A vast majority of people still align artificial intelligence with sci-fi dystopias. However, truth be told, that characterization of this disruptive technology is waning down…
A vast majority of people still align artificial intelligence with sci-fi dystopias. However, truth be told, that characterization of this disruptive technology is waning down…
The question of whether IoT can be used to drive social impact has been revolving around for a long time. However, the answer arrived from…
For a long time, the idea of a truly connected world existed only in theories. But with the recent advancements in the field of IoT,…
Steering a business towards success requires one to focus on a vision, spur innovation, and maintain a level of efficiency to drive profitable growth. Mr.…
What makes some businesses tick and others fail? There are some features that bind together all great names. It is not always about the…
NEW YORK – U.S. authorities on Friday announced criminal charges against the alleged leaders of an multibillion-dollar pyramid scheme involving the sale of a fraudulent cryptocurrency, OneCoin.
Konstantin Ignatov, who runs OneCoin Ltd, and his older sister Ruja Ignatova, who co-founded OneCoin and is known as "Cryptoqueen," were accused of swindling investors coveting big returns and low risk, in a scheme conceived as a fraud.
Prosecutors said OneCoin, which still operates, claimed to have more than 3 million members, who would receive commissions for recruiting new members to buy cryptocurrency packages.
Much of the $1.2 billion of investor money located so far was laundered through at least 21 countries, prosecutors said.
OneCoin records, meanwhile, show profit of 2.23 billion euros (US$2.51 billion) on sales of 3.35 billion euros (US$3.77 billion) in the two years ended Sept. 2016, they added.
The defendants "created a multibillion-dollar cryptocurrency company based completely on lies and deceit," U.S. Attorney Geoffrey Berman in Manhattan said in a statement. "Investors were victimized while the defendants got rich."
Manhattan District Attorney Cyrus Vance added: "These defendants executed an old-school pyramid scheme on a new-school platform."
Ignatov, 33, of Sofia, Bulgaria, was charged with wire fraud conspiracy, and detained after being arrested on Wednesday at Los Angeles International Airport.
Ignatova, 38, also of Sofia, was charged with wire fraud, securities fraud and conspiracy. She has been at large since disappearing in Oct. 2017.
Lawyers for the siblings could not immediately be located.
According to court papers, the defendants misled investors into believing OneCoin's value was determined by supply and demand, when it was actually determined internally, and that OneCoins were "mined" using company servers.
The papers also include quotations suggesting the defendants knew OneCoin was a fraud.
Ignatova was quoted as telling a co-founder that "I cheat currently on coins," and that one possible "exit strategy" was to "take the money and run and blame someone else."
Ignatov, meanwhile, allegedly told a OneCoin member recently in Las Vegas: "If you are here to cash out leave this room now because you don't understand what this project is about."
Another defendant, Mark Scott, 50, of Coral Gables, Florida, pleaded not guilty last September to conspiring to launder more than $400 million for OneCoin.
Scott is a former partner at the law firm Locke Lord.
"Unequivocally, Mr. Scott maintains his complete and utter innocence," his lawyer David Garvin said on Friday. "His conduct is consistent with the normal, proper duties of a lawyer."
The case is U.S. v. Scott et al, U.S. District Court, Southern District of New York, No. 17-cr-00630.
Source: Reuters


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Almost nothing is known about the identity of the genes causing reproductive isolation between species. As a first step towards molecular isolation of a 'speciation gene', I mapped and partly characterized a gene causing hybrid male sterility in Drosophila. This analysis shows that sterility of D. melanogaster males who carry the 'dot' fourth chromosome from D. simulans is due entirely to a very small region of the D. simulans chromosome (including only about 5 salivary gland bands or approximately 250 kb of DNA). Thus the hybrid sterility effect of the D. simulans fourth chromosome is almost surely due to a single gene of very large effect (here named hms, hybrid male sterile). Hms is zygotically acting, and the D. simulans allele of hms is completely recessive. Furthermore, complementation tests suggest that hms is not an allele of any known locus in D. melanogaster.
Recombination between F42lac and λplac5 is typically 20- to 50-fold more efficient than recombination between chromosomal lac and λplac5. This enhancement of recombination is recBCD-dependent and requires the expression of genes from the tra regulon of the F factor. Also required is oriT, the origin of F factor conjugational transfer, which must be located in-cis to the cellular copy of lac. In this study we show that enhanced recombination is not supported by an oriT point mutant that reduces oriT function in conjugation. We also present evidence that the activation of oriT for recombination enhancement involves the same strand-specific nick that is required for conjugal DNA transfer. Although it is thought that the role of oriT in recombination enhancement is related to the facilitated entry of RecBCD enzyme into the DNA duplex, we were unable to detect any double-strand breakage at oriT.
The accumulation of a transposable element inside chromosomal inversions is examined theoretically by a mathematical model, and empirically by counts of P elements associated with inversion polymorphisms in natural populations of Drosophila melanogaster. The model demonstrates that, if heterozygosity for an inversion effectively reduces element associated production of detrimental chromosome rearrangements, a differential accumulation of elements is expected, with increased copy number inside the minority inversion. Several-fold differential accumulations are possible with certain parameter values. We present data on P element counts for inversion polymorphisms on all five chromosome arms of 157 haploid genomes from two African populations. Our observations show significantly increased numbers of elements within the regions associated with the least common, or minority arrangements, in natural inversion polymorphisms.
At the white eye colour locus, there are a number of alleles that have altered expression between males and females. To test these regulatory mutations of the white eye colour locus for their phenotypic expression in metafemales (3X; 2A) compared to diploid females and males, eleven alleles or transduced copies of white were analysed. Two alleles that exhibit dosage compensation between males and females (apricot, blood) also exhibit dosage compensation in metafemales. White-ivory and white-eosin, which fail to dosage compensate in males compared to females, but that are distinct physical lesions, also show a dosage effect in metafemales. Two alleles with greater expression in males than females (spotted, spotted-55) exhibit even lower expression in metafemales. Lastly, five transduced copies of white carrying three different lengths of the white promoter, but that all exhibit higher expression in males, show reduced expression in metafemales, exhibiting an inverse correlation between the level of expression and the dosage of the X chromosome. Because these alleles of white respond to dosage compensation in metafemales as a continuum of the male and female responses, it is concluded that the same basic mechanism of dosage compensation is involved and that the dosage of the X chromosome conditions the sexually dimorphic expression.
Current models based on the analysis of linear metabolic pathways at steady-state predict that large increases over wild type in the activity of one enzyme will not alter an organism's fitness. This prediction is tested at steps in a highly branched pathway under two conditions known to alter steady-state: heat shock and nitrogen starvation. Saccharomyces cerevisiae transformants overproducing 1 of 4 enzymes in glycolysis (hexokinase B, phosphoglucose isomerase, phosphofructokinase, or pyruvate kinase) were subjected to heat shock in both exponential and stationary phases of growth. In neither phase does enzyme overexpression alter heat shock sensitivity. When starved for nitrogen in acetate medium, transformants overproducing hexokinase, phosphoglucose isomerase, and phosphofructokinase sporulate at the same rate and with the same frequency as cells harbouring only the plasmid vector. Current models therefore correctly predict the relationship between activity and components of fitness for 3 of 4 enzymes. By contrast, cells overexpressing pyruvate kinase sporulate poorly. This defect is not observed among cells transformed with a plasmid containing a Tn5 disrupted copy of the PYK gene. These findings are consistent with reports that implicate the PYK locus in yeast cell cycle control and suggest that it may be challenging to model relations between fitness and activity for multifunctional proteins.
By designing 3′ ends of primers in PCR (polymerase chain reaction), a specific DNA fragment was selectively amplified in the presence of a 103-fold excess of highly homologous (sequence difference ca. 2 %) opponent DNA. This technique was applied in detecting paternal leakage of mitochondrial DNA (mtDNA) in intraspecific crosses of Drosophila simulans and interspecific crosses of Drosophila simulans and Drosophila mauritiana. The mtDNA types of their progeny were analysed by selective amplification of the paternal mtDNA fragment possessing a polymorphic restriction site and detecting its cleaved fragments. Paternal mtDNA was detected in the progeny of 14 out of 16 crosses. The present result indicates small but frequent inheritance of sperm mtDNA in Drosophila, which is supportive to our previous finding.
The Drosophila melanogasterecd1 mutation causes a severe temperature-sensitive deficiency in the titre of the steroid hormone eedysone. This mutation was used to investigate the role of eedysone in both the transcription of the genes mapped at the 3C11–12 intermoult puff region and the puff formation. Thoroughly synchronized ecd1 larvae were shifted to the non-permissive temperature at various times of the development; after 24 or 48 h, the levels of the transcripts derived from Sgs-4, Pig-1 and ng-1, the three genes located at the 3C11–12 polytene bands, were determined. The results showed that the levels of the transcripts encoded by Pig-1 and ng-1 are unaffected by the drop in the ecdysone titre occurring in non-permissive conditions whereas the amount of Sgs-4 mRNA is greatly reduced. These data clearly indicate that transcription of the three genes mapped within the puff region is affected differently by the hormone. Furthermore, ecd1 larvae cultured at the non-permissive temperature show a prominent puff at the 3C11–12 polytene bands, indicating that eedysone is not essential for puff induction and that puff size is not simply correlated with high-level Sgs-4 transcription.
The number and organization of amylase genes in Drosophila ananassae were investigated through classical genetic methods and in situ and filter hybridizations. At least four genes may be active in D. ananassae, organized as two independent pairs of closely linked copies on the 2L and 3L chromosomal arms. Several other species of the D. ananassae subgroup were studied and show the same chromosomal locations, suggesting an ancient duplication event. However, the number of Amy copies seems to be higher in the D. ananassae multigene family, and there is a striking intraspecific molecular differentiation.
Extracts of disomic wheat-barley addition lines were tested for the presence of a barley malt endopeptidase (MEP-1) by employing isoelectric focusing (IEF) and western blotting. The blots were probed with polyclonal antibodies raised against MEP-1 purified from the endosperms of 5-day-old germinated barley seedlings. The endopeptidase was detected in the Betzes barley cultivar and the addition line containing the full genome of the wheat cultivar Chinese Spring plus a chromosome 3 pair from Betzes barley. The endopeptidase was not expressed in Chinese Spring nor the addition lines containing other Betzes chromosome pairs. The endopeptidase was detected in a ditelosomic addition line containing the long arm of Betzes chromosome 3. We have concluded that the gene coding for MEP-1 (Cep-B) is located on the long arm of Betzes chromosome 3.
Levels of transcripts produced by a heat shock protein 70 (hsp70)-antisense white transgene in Drosophila were measured after single and multiple heat shocks to determine whether the hsp70 promoter could produce sustained high levels of transgene transcripts. A single heat shock resulted in typical highly inducible levels of RNA, but the amount of antisense RNA was substantially reduced after multiple heat shocks. Endogenous hsp70 mRNA levels were also less abundant after multiple heat shocks as compared to a single heat shock. The hsp70 promoter is unsuitable for use in fusion gene constructs for long term expression studies where repeated heat shocks are required.
Recessive lethal germline and specific locus somatic mutations were induced efficiently in the zebrafish by exposure of mature sperm to UV light. Mutagenesis of sperm yielded mosaic individuals: clones bearing novel mutations represented approximately 12–25% of the haploid germ cells and 25–50 % of the somatic tissue. Simple methods are described for the reliable identification and propagation of newly arising developmental mutations in zebrafish.
An allele of the mouse brachyury locus, T22H, had been shown previously to involve a deletion of several markers in the proximal part of chromosome 17, and almost certainly includes deletion of the t-complex distorter gene Tcd-1. The effects of T22H on transmission ratio distortion and male sterility caused by the t-complex were compared with those of a partial t-haplotype th51, which carries the t-form of the distorter Tcd-1t. In combination with the complete haplotypetf32, T22H caused severe impairment of male fertility, but males of genotype T22H/t6 or T22H/thSl were normally fertile. These results were very similar to those obtained when th51 was in combination with the same haplotypes. In effect on transmission ratio T22H was again similar to thSI, in that it produced a marked increase in the transmission of the haplotype t6. To test whether the effects of T22H were due to deletion of elements other than Tcd-1, the effect of T22H on transmission of the partial haplotype th2 was compared with that of the deletion Thv. Again T22H markedly increased transmission of the t-haplotype and the effect was significantly greater than the small effect produced by Thp.
It is concluded that deletion of the distorter Tcd-1 has an effect like that of the t-form of this distorter, Tcd-1t, and hence that Ted-11 must be an amorph or hypomorph. It is speculated that other t-complex distorters, Tcd-2t and Tcd-3t may also be amorphs or hypomorphs. Thus, the phenomena of transmission ratio distortion and male sterility due to the t-complex appear to be brought about by differential susceptibility of wild-type and t-responder alleles, Tcr+ and Tcrt, to a shortage of distorter gene product.
In Drosophila, sex is determined by the relative number of X chromosomes to autosomal sets (X: A ratio). The amount of products from several X-linked genes, called sisterless elements, is used to indicate to Sex-lethal the relative number of X chromosomes present in the cell. In response to the X: A signal, Sex-lethal is activated in females but remains inactive in males, being responsible for the control of both sex determination and dosage compensation. Here we find that the X-linked segmentation gene runt plays a role in this process. Reduced function of runt results in femalespecific lethality and sexual transformation of XX animals that are heterozygous for Sxl or sis loss-of-function mutations. These interactions are suppressed by SxlMI, a mutation that constitutively expresses female Sex-lethal functions, and occur at the time when the X: A signal determines Sex-lethal activity. Moreover, the presence of a loss-of-function runt mutation masculinizes triploid intersexes. On the other hand, runt duplications cause a reduction in male viability by ectopic activation of Sex-lethal. We conclude that runt is needed for the initial step of Sex-lethal activation, but does not have a major role as an X-counting element.
Recessive lethal mutations and mutations at the gol-1 locus were induced in the zebrafish by exposure of mature sperm to the alkylating agent ethyl nitrosourea (ENU). Embryonic lethal phenotypes were recognized among the parthenogenetic progeny of mutagenized animals or among the progeny of daughters of mutagenized animals. Novel specific locus mutations were identified by the failure of mutagenized chromosomes to complement pre-existing mutant alleles at the gol-1 locus. Each mutagenized individual harboured approximately 10 embryonic lethal mutations in its germ line and about 1 in 500 mutagenized animals harboured a new mutation at the gol-1 locus. Three lines of evidence indicate that the majority of mutations that were recovered following treatment of mature sperm with ENU were probably point mutations. First, the soma and germ lines of mutagenized animals were mosaic, as expected following simple alkylation of sperm DNA. Second, mutations induced by ENU at the gol-1 locus affected pigmentation but not viability, unlike the majority of mutations induced at this locus with y-irradiation. Third, the ratio of specific locus: recessive lethal mutations induced by ENU was approximately 50-fold lower than the ratio observed following mutagenesis with y-rays. Comparison of the incidence with which embryonic recessive lethal mutations were induced with the incidence with which specific locus mutations arose indicates that there are greater than 5000 genes essential to the development and viability of the zebrafish embryo.
Current models predict that large increases over wild-type in the activity of one enzyme will not alter an organism's fitness. This prediction is tested in Saccharomyces cerevisiae through the use of a high copy plasmid that bears one of the following: hexokinase B (HEXB), phosphoglucose isomerase (PGI), phosphofructokinase (PFKA and PFKB), or pyruvate kinase (PYK). Transformants containing these plasmids demonstrate a four to ten-fold increase in enzyme specific activity over either the parent strain or transformants containing the plasmid alone. Haploid and diploid transformants derived from independent backgrounds were grown on both fermentable and non-fermentable carbon sources and evaluated for several components of fitness. These include growth rate under non-limiting conditions, maximum stationary phase density, and viability in extended batch culture. Cell viability is not affected by overproduction of these enzymes. Growth rate and stationary phase density do not differ significantly among strains that overexpress HEXB, PGI or contain the vector alone. PFKA, B transformants show reduced growth rate on glucose in one background only. For these loci the current model is confirmed. By contrast, when grown on glucose, yeast overexpressing PYK demonstrate reduced growth rate and increased stationary phase density in both backgrounds. These effects are abolished in cells containing plasmids with a Tn5 disrupted copy of the PYK gene. Our results are consistent with reports that the PYK locus may exert control over the yeast cell cycle and suggest that it will be challenging to model relations between fitness and activity for multifunctional proteins.
Fifteen autosomal dominant mutations that cause cataract of lenses in mice were tested for allelism. The outcrosses of double mutants revealed three allelism groups, consisting of 5, 4 and 2 mutations as well as 4 mutations which segregated independently. The results indicated 7 different cataract loci in the sample of 15 mutations. The biomicroscopic examination of the eyes showed that phenotypically similar as well as very distinct cataract mutations can be alleles of the same gene. Conversely, phenotypically similar mutations were shown to be non-allelic.
Using a stochastic model of a finite population in which there is mutation to partially recessive detrimental alleles at many loci, we study the effects of population size and linkage between the loci on the population mean fitness and inbreeding depression values. Although linkage between the selected loci decreases the amount of inbreeding depression, neither population size nor recombination rate have strong effects on these quantities, unless extremely small values are assumed. We also investigate how partial linkage between the loci that determine fitness affects the invasion of populations by alleles at a modifier locus that controls the selfing rate. In most of the cases studied, the direction of selection on modifiers was consistent with that found in our previous deterministic calculations. However, there was some evidence that linkage between the modifier locus and the selected loci makes outcrossing less likely to evolve; more losses of alleles promoting outcrossing occurred in runs with linkage than in runs with free recombination. We also studied the fate of neutral alleles introduced into populations carrying detrimental mutations. The times to loss of neutral alleles introduced at low frequency were shorter than those predicted for alleles in the absence of selected loci, taking into account the reduction of the effective population size due to inbreeding. Previous studies have been confined to outbreeding populations, and to alleles at frequencies close to one-half, and have found an effect in the opposite direction. It therefore appears that associations between neutral and selected loci may produce effects that differ according to the initial frequencies of the neutral alleles.
Two cDNA clones coding for two forms of the mouse kidney androgen-regulated protein (KAP) distinguished by their electrophoretic mobilities on SDS gel electrophoresis have been isolated from libraries prepared from strains of mice having one (BALB/c) or two (OFl) forms of the KAP protein. The corresponding mRNAs have identical sizes, as well as identical sequences in their 5' non-translated regions. The size difference observed between the two proteins is due to two point mutations in the coding region of the KAP mRNA, leading to two amino-acid changes one of which resulted in the substitution of a glycine for a glutamic acid. As shown by in vitro transcription/translation experiments, these two amino-acid differences are responsible for the shift in the apparent molecular weight of the protein on SDS gels. Both forms of the protein are more abundant in males than in females.
In vitro translation of kidney RNAs isolated from six different strains and species of mice revealed the presence of other forms of the KAP protein, characterized by small variations of their molecular weights. Southern blot analysis data are consistent with the presence of only one kap gene in the mouse genome. A restriction fragment length polymorphism has been observed, which does not correlate with the protein polymorphism, indicating the presence of another allele in the OF1 mouse genome.
Using a mouse cDNA probe encoding for the major part of peripherin, a type III intermediate filament protein, we have assigned, by in situ hybridization, the mouse and human peripherin genes, Prph, to the E–F region of chromosome 15 and to the q12–q13 region of chromosome 12, respectively. These regions are known as homologous chromosomal segments containing other intermediate filament genes (keratins) and also other genes which could be co-ordinately regulated.


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'What a hypocrite!' Social media slams Today show host Karl Stefanovic for 'double standards' after his rant over pictures of himself checking into caravan park with 12 cans of rum
- Today show host went on a tirade after pictures of him were published online
- They show him buying drinks before checking into accommodation in Yamba
- Viewers accused him of double standards, given his own show's news coverage
- Marks the end of a controversial week in which he mocked Today's royal coverage and insisted he 'didn't care' about media reporting of Schapelle Corby
Social media has slammed Karl Stefanovic as a 'hypocrite' after he went on a rant on the Today show after pictures of himself checking into a country caravan park carrying 12 cans of pre-mixed rum were published.
In a segment on Friday morning's show, the host - who earns a reported $3million a year - unleashed a tirade against stories 'denigrating' women.
It came after the publication of pictures which showed the 42-year-old stopping by a bottle shop to pick up drinks on the way to his accommodation in Yamba, on the NSW north coast, in late April accompanied by a Channel Nine producer.
But viewers who watched his rant called him out for having 'double standards,' citing examples of his own work on the breakfast program.
'Double standards when it hits your own team!' one woman wrote on Facebook.
Scroll down for video
Karl Stefanovic went on a tirade on The Today show after pictures of himself checking into a country caravan park carrying 12 cans of pre-mixed rum were published
His outburst provoked a strong reaction on social media, with some users taking to Twitter and Facebook to call Stefanovic out as a hypocrite
'Didn't you guys run with the Jennifer Aniston pregnant [sic] because she had a little tummy or the Nicole Kidman pregnancy and handclapping at the Oscars.
'You guys run every story they do except when it's about your own people. It's your business or you'd be out of a job.'
Another woman added: 'What a hipocrit [sic] you are Karl. It's alright for you to bag the likes of Pauline Hanson and hound other people just to get a story but when it comes to your own you don't like it.
'Talk about double standards! What a joke!'
'What a load of crap coming from a "man" who treated his wife in such a deplorable manner,' one person wrote on Twitter.
The 42-year-old stopping by a bottle shop to pick up the drinks on the way to the park in Yamba, located north of Sydney, in late April accompanied by a Channel Nine producer
Twitter users called the Today show presenter out for being a hypocrite after his tirade
One Twitter user said: 'So Channel 9 has never done anything like this in the past? Pull your head in.'
Another added: 'Thing is you guys do exactly the same thing to others! Pot calling the kettle black... it's all about shock value and viewer numbers. Admit it.'
The pictures of Stefanovic, who split from his wife of 21 years Cassandra Thorburn last year, show him in a white shirt and black skinny jeans at the bottle shop.
Before checking into the caravan park, he was spotted as he stopped by a store where he purchased 12 cans of pre-mixed alcoholic rum beverages.
A Channel Nine spokesperson told Daily Mail Australia on Thursday that Stefanovic was in Yamba 'filming a story about the crisis in the prawn industry for the Today show.'
This was carried prominently alongside the pictures.
Yet Stefanovic bizarrely suggested the pictures were an attack on women because he was carrying the rum cans before entering the caravan park with his female colleague.
'Fact, this was work. We were filming a story about our struggling prawn farmers, they deserve a rum or two,' he said.
Besides his duties on the breakfast program, Stefanovic occasionally works on special news projects for the network such as reports for 60 Minutes.
Stefanovic said he was on the NSW north coast with his colleague for a story about the prawn industry
The rant comes at the end of a week of combative comments from Stefanovic.
Like most of Australia's media, his bosses invested huge amounts of resources in covering the return of Schapelle Corby to her home following her drugs conviction in Bali.
Yet Stefanovic insisted he 'didn't care' about the media circus surrounding a story which has gripped the public.
And he wasn't finished there. He then mocked the Today show's 'royal scoop' featuring footage of Pippa Middleton's husband James on the balcony of their Sydney hotel.
Stefanovic reportedly left his wife with $6 million in cash and assets plus child support and 'ongoing payments, according to The Sunday Telegraph.
Meanwhile, the Today host was said to have walked away from their 21-year marriage with just $500,000 in financial assets - an amount that included his white Mercedes.
As part of their divorce settlement, Stefanovic and Ms Thorburn will share custody of their three children – Jackson, 17, Ava, 12, and River, 10.
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A human-rights group and the family of a mentally-ill man on death row in Pakistan have pleaded with the government to halt his execution.
Imdad Ali was convicted in a 2001 murder case and has exhausted all appeals.
A clemency request to Pakistan's president was rejected, and he is due to be executed on Tuesday.
Justice Project Pakistan said on Sunday that state-appointed doctors diagnosed Ali with schizophrenia and declared him unfit to be hanged.
His wife, Safia Bano, has called on the government to intervene.
The Justice Project says Pakistan has executed 419 people since it lifted a ban on capital punishment in December 2014.
Here are the results of Imdad's medical evaluation in 2012. His condition has only worsened since. #SaveImdad pic.twitter.com/ZD7lmIj910— Justice Project Pakistan (@JusticeProject_) September 17, 2016


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