text:
This time it's the Septics that are neglected:
http://news.bbc.co.uk/1/hi/world/americas/7085081.stm for the full piece.
http://news.bbc.co.uk/1/hi/world/americas/7085081.stm for the full piece.
Veterans 'quarter of US homeless'
The report calls for more support for military veterans
One in four homeless people in the US is a military veteran, even though veterans make up only 11% of the adult population, a report has found.
The National Alliance to End Homelessness said that on any given night in 2006, some 194,254 out of 744,313 homeless people were veterans.
The non-profit group called the figures "shockingly disproportionate".
In its report, the group also said that 495,400 veterans were homeless at some point in 2006.
The report, Vital Mission: Ending Homelessness Among Veterans, said that veterans made up 26% of the homeless population.
In addition, 44,000-64,000 veterans were chronically homeless, and just under 500,000 veterans were "at a high risk" of homelessness.
The findings are based on figures from the Department of Veterans Affairs and the Census Bureau.


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Influencer collaborations continue to be on the rise, with a collection between handbag label Mykilim—also known as Mklm—and fashion influencer Rocky Barn
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Peer to peer selling may be the next big move for fashion and tech entrepreneurs, but any new platform will have to have full transparency when it comes to
Dec 27, 2018

subset: commoncrawl


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UK General Election and next steps for Brexit
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With a significant majority the new Conservative Government appears to be on…Continue Reading
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text: Cronley's sports-oriented farces (Fall Guy, Screwballs) have been marginally musing, but this derivative attempt at crime comedy is just plain feeble. The hero is a guy named Grimm who dresses in a clown suit and robs a bank, assisted by cohorts Phyllis (""great legs"") and numbskull Lackey. But Grimm accidentally shoots the sprinkler system and starts a downpour, so he hustles everyone into the vault, makes hostage demands, and then disappears (by quick-changing himself into being one of the hostages). Despite this getaway, however, the comedy of errors escalates: Lackey is such a nitwit that he can't find the West Side highway; the trio winds up in Harlem at a deadly gambling bar; and when they get to Grimm's apartment, a former crime-colleague ambushes them and grabs $30,000 of the bank loot. So, while incompetent Police Chief Rotzinger mounts a campaign to find them, the bumbling trio takes a wild cab (Grimm's car has been demolished by firemen) and then a bus to the airport. And after more imbroglios they finally land in England, but a stewardess has made off with their cash-filled suitcase; so we last see them once more doing the clown caper on an English bank. Lame and leaden stuff: re-read The Gang That Couldn't Shoot Straight--or anything by Donald E. Westlake--instead.


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The Last Of Us 2 Release Date Confirmed
Plus a trailer and an unexpected visitor...
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Six years since The Last of Us made gamers everywhere ugly cry, Naughty Dog and Sony have finally announced the release date of the highly anticipated sequel, The Last of Us Part II.
Mark February 21 2020 in your calendars, as the PlayStation State of Play live stream has confirmed that Ellie and Joel will be returning to our consoles in the new year.
Watch the trailer here, and try not to gasp as loud as we did at that reveal.
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Ultrastructural localization of lectin receptors in the preimplantation ovine embryo.код для вставкиСкачать
THE ANATOMICAL RECORD 240:537-544 (1994) Ultrastructural Localization of Lectin Receptors in the Preimplant ation Ovine Embryo P. DE PAZ, A.J. SANCHEZ, J.G. FERNANDEZ, C. GARCIA, C.A. CHAMORRO, AND L. ANEL Departamento de Biologia Celular y Anatomia (P.D.P., A.J.S., J.G.F., C.G., C.A.C.) and Departamento de Patologia Animal (Sanidad) (L.A.), Facultad de Veterinaria, Universidad de Leon, Leon, Spain ABSTRACT Background: Preimplantation development of mammalia is characterized by cell surface changes functioning in intercellular communication and adhesion. The glycoconjugate role in cellular interactions has been analysed for several groups but not in sheep embryos. The binding patterns of eleven lectins during sheep preimplantation development were investigated and the role of glycoconjugates in early development was discussed. Methods: Ultrathin sections from preimplantation ovine embryos (3-7 days) were incubated with different colloidal gold conjugated lectins and the frequency of gold particles on the cell membrane, some organelles, and the zona pellucida was evaluated. Results and Conclusions: We observed a higher staining of WGA, DBA, and SBA lectins in the intercellular contact zone with respect to the free cell surface of blastomeres during cleavage. This indicates that the N-acetyl galactosamine and N-acetyl glucosamine residues may be involved in sheep morula compaction. In contrast, the trophoblast cell displays an increase of staining of some lectins previously identified during cleavage (LcH, WGA, SBA, MPA, and PNA) on the free membrane, and a lack of sugar residues in the intercellular surface. This polarization of the trophoblast cell surface is not observed in the inner cell mass and could provide a mechanism for differentiation within the blastocyst. Intracytoplasmic vesicles show a cytochemical identity with lysosomes in the blastocyst (abundant GlcNAc and Man/Glc residues) that may reflect a functional relationship between both organelles in an intracellular cycle. The zona pellucida presents abundant GalNAc, GlcNAc, and Gal residues during preimplantation ovine development. 0 1994 Wiley-Liss, Inc. Key words: Lectins, Sheep, Preimplantation embryo The nature of the molecules present in the cell membrane and its more extrinsic component is of considerable interest because of the unique position of the cell surface a t the interface between a cell and its external environment. In particular it is important in the understanding of morphogenesis to characterize the changes occurring in cell surface components during development. In most instances, efforts have focussed on the protein components, though it has been suspected for some time that cellular interactions are dependent upon complex carbohydrates. The critical role of glycoconjugates in cellular interactions has been clearly defined by different investigators (Gooi et al., 1981; Sharon and Lis, 1989; Peacock et al., 1990; Brandley, 1991). Cell surface changes in cells and tissue including the preimplantation mammalian embryo have been investigated with lectins (Lis and Sharon, 1986; Dealtry and Sellens, 1987; Spicer and Schulte, 1992). The charac0 1994 WILEY-LISS, INC. terization of the glycoconjugate composition of blastomeres during early development may provide clues a s to the macromolecules functioning in intercellular communication and changes in cell behavior (Shur, 1989; Fenderson and Eddy, 1990). Early development of mammalian embryo is characterized primarily by a major morphological event termed compaction that causes a major reorganization among the cells of the morula. Compaction is a complex process in which polarization of blastomeres occurs, cell-cell contacts are maximized, and specialized junctions formed (Rastan el al., 1985; Wiley, 1988; Watson, 1992). This process is a prerequisite for blastocyst for- Received February 1, 1994; accepted June 22, 1994. Address reprint requests to Paulino De Paz, Departamento de Biologia Celular y Anatomia, Facultad de Veterinaria, Universidad de Leon, 24071 Leon, Spain. 538 P. DE PAZ ET AL TABLE 1. Description of the lectins used in this study and their specificities' Lectin Concanavalia ensiformis Abbreviation ConA Specificity' Man a1,2 Man a1,2 Man > Man a1,2 Man > a Man > a Glc > a GlcNAc con^.^ 1:15 Sugar (0.2 M) D-Man 1:15 D-Man 1:lO D-GlcNAc Source Sigma Chemical co. (St. Louis, MO) E-Y Laboratories (San Mateo, CAI E-Y Laboratories 1:lO D-GlcNAc Lens culinaris Lch a Man > a Glc > a GlcNAc Griffonia simplicifolia 11 Triticum vulgare GS-I1 a GlcNAc = WGA Dolichus biflorus DBA Glycine max SBA GlcNAc p1,4 GlcNAc p1,4GlcNAc > GlcNAc p1,4 GlcNAc >> GlcNAc GalNAc a1,3 GalNAc > a GalNAc >> a Gal a GalNAc = p GalNAc > a Gal GalNAc > Gal Gal p1,3 GalNAc > GaINH, > Gal a-L-Fucosa a-L-Fucosa Maclura pomifera MPA Arachis hypogaea PNA Ulex europaeus I Lotus tetranoglo bus Limulus polyphemus UEA-I Lotus LPA 13 GlcNAc NeurGc a 2,3 GalNAc > NeurAc a 2,6 GalNAc > NeurAc = NeurGc Diluent Buffer pH 0.01 M PBS 7-8 0.15 M NaCl 0.1 mM Ca2+ and Mn2+ 0.05 M TRIS 7.2 0.15 M NaCl 0.01 M PBS 7.45 E-Y Laboratories 0.01 M PBS 7.45 1:lO D-GalNAc E-Y Laboratories 0.01 M PBS 7.45 1:lO D-GalNAc E-Y Laboratories 0.01 M PBS 7.45 1:lO 1:lO D-Gal D-Gal E-Y Laboratories E-Y Laboratories 0.01 M PBS 0.01 M PBS 7.45 7.45 1:lO 1:lO L-FUC L-FUC E-Y Laboratories E-Y Laboratories 0.01 M PBS 0.01 M PBS 7.45 7.45 NeurNAc E-Y Laboratories 0.05 M T R I S 0.15 M NaCl 0.1 mM Ca'+ 8 1:5 'For each lectin the dilution (Conc.),inhibiting sugar, and diluent solution are indicated. The buffers used are the ones recommended by the commercial laboratories. 'After Goldstein and Poretz (1986). 3Referred to commercial solutions. mation in which two distinct cell populations can be catheter. The embryos were classified morphologically distinguished: the trophectoderm and the inner cell as excellent to poor according to Lidner and Wright mass. There is abundant evidence that glycoconjugates (1983) and only those with a quality grade of 1 or 2 are involved in these processes (Gooi et al., 1981; Kim- were analyzed. ber and Bagley, 1987; Bayna et al., 1988; Hathaway et al., 1989; Bukers a t al., 1990). L ectin Staining In this paper we have investigated the binding pattern of 11 lectins during preimplantation development The embryos were fixed with a mixture of formaldeof sheep embryos and discussed the role of glycoconju- hyde (4%) and glutaraldehyde (0.25%) in PBS for 1 hr gates in early development. a t 4°C and immersed in 0.5 M ammonium chloride for another 30 min. After dehydration in a graded series of MATERIALS AND METHODS ethanol (30, 50, 70, 90, and 100%) a t decreasing temEmbryo Collection peratures (0, -20, -30, -30, and -3O"C, respectively) Adult Churra ewes were pretreated with fluoroge- the pieces were infiltrated and embedded in Lowicryl stone acetate (FGA; Chronogest, Intervet, Holland) 30 K4M at -25°C for 24 h r and 20°C for 72 hr. mg intravaginal pessaries for 12 days. Sixty hours beThe sections were then cut with glass knives on an fore sponge removal donors were superovulated with ultratome LKB V and mounted on 150 mesh nickel 12 mg of follicle stimulating hormone-pituitary extract grids having a formvar film. Grids were preincubated (FSH-P,Shering Corp., Kenilworth, NJ, USA) with the for 15 min on a drop of 0.5 M glycine in PBS and incufollowing dose: 4, 4,3, 3, 2, and 2 mglanimal at 12 hr bated on a drop of the gold labelled lectin for 6 hr in a intervals. At the time of fifth dosage, 400 IU pregnant moist chamber a t room temperature according to Table mare serum gonadotrophin (PMSG, Foligon, Intervet, 1. At the end of the labelling procedure the sections Holland) were administered. Ewes were both naturally were washed with PBS, rinsed in doubly-distilled wamated and artificially inseminated 12-24 h r after the ter, and dried. The grids were stained with uranyl aconset of estrus. etate and lead citrate. The embryos were recovered surgically 3 to 7 days The colloidal gold-conjugated lectins and the inhibafter sponge removal. The uterine horns or oviduct iting sugars used in this study are shown in Table 1. were flushed with DPBS + 15% FCS using a Foley Preliminary studies with sugar a t different concentra- 539 LECTIN RECEPTORS IN EARLY OVINE EMBRYO TABLE 2. Staining patterns (mean 2 s.d.) showed by the lectins used in this study in the different stages of ovine Dreimdantation develoDment' Cell membrane (uipm) Cleavage Lectin ConA LcH GS-I1 Free Interc 10 (2.1) 4 (2.4) 12 (2.6) - - - WGA 25(6.4) 42 (12.3) DBA 10 (2) 35 (5.9) SBA 8 (2.9) 40 (8) MPA 4(0.5) 4 (0.3) PNA 2(0.5) 4 (0.4) UEA-I 611.6) Lotus LPA - Organelles (uipm2) BICM Cleavage B Troph BICM Zona pellucida ( d p m 2 ) Free Interc Interc Vesic Lysos Vesic Lysos Vesic Lysos Cleav Blast 8 (2.1) 6 (1.9) 72 (17.9) 18 (3.8) 80 (26.1) 14 (6.4) 78 (13.3) 16 (9.2) 32 (11.3) 30 (3.7) 69(11.2) 4(1.4) 4(1.5) 26(9.1) 29 (13.6) 26 (4.4) B Troph - - - - 2 (0.5) 8(1.4) 86(18.3) - - - - - - 18 (2) 210 (10) 34 (2) 40 (4) 36 (4.2) 40 (3.3) 20 (8) 230 (16) 14 (3.1) 6 (2) 28 (6.8) 34 (2.3) - - - - 6(1.2) 6(1.1) 4(0.5) 4(1.6) - - - - - - 4 (1.4) 4(1.2) 4(1.3) 8(1.1) - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 16(5.2) - - - - - - - - - - - ~ 32 (11.8) 120 (13) 28 (13.5) 140 (7.2) 74(15.9) 8 (3.4) 37 (7.2) 30(6) 28(4.1) 8(0.5) - 'These data refer to the free and intercellular (Interc) membrane (colloidal gold particlesipm), the cell organelles (gold colloidal particlesipm'), and the zona pellucida (gold colloidal particlesipm'). > 100 particlesiu = very heavy; 50-100 = heavy; 25-50 = light; < 25 = sparse;-= 0. Vesic = intracytoplasmic vesicles; Lysos = lysosomes; Cleav = cleavage; B Troph = blastocyst trophectoderm; BICM = blastocyst inner cell mass; Blast = blastocyst. tions were carried out in order to assess the carbohydrate specificity of the lectin to the embryonic cells. In the whole series of experiments we used grids with sections from the same embryo a s a control for the specific binding of the lectins. These sections were incubated with the lectin-gold complex previously mixed with the corresponding specific sugar for 1 h r before use (see Table 1). Six embryos from each stage were used, six ultrathin sections per embryo were incubated with lectin and six micrographs per section were obtained by random sampling. The frequency of gold particles per square micron (organelle and zona pellucida) or per micron (plasma membrane) was determined by counting on the electron micrographs. A double square lattice ((264) is superimposed on each micrograph to estimate the area of cellular organelles and the length of membrane profiles (Weibel, 1979). The gold particle number of each structure was counted and this count was standardised to 1 pm2 (organelle) or 1 pm (plasma membrane) and the means and standard error of means calculated. RESULTS We analyzed several developmental stages of ovine embryos up to blastula stage by means of colloidal gold conjugated lectins. The results refer to the frequency of the gold particles on the cell membrane (the free cell surface and the intercellular membrane were distinguished), some organelles (intracytoplasmic vesicles and lysosomes), and the zona pellucida. Cleavage The results obtained during the cleavage period have been analyzed as a whole although we carried out a complete study of 8-cell stage, early morula and compact morula. Throughout these stages neither qualitative nor quantitative differences were found, and thus the data are summarized a s a whole in Table 2. On the free cell surface a sparse staining appeared for LcH, DBA, SBA, MPA, and PNA lectins and a light staining for WGA lectin. The intercellular contact surface of the ovine embryonic cells during cleavage (Fig. la,b) showed light quantity of GalNAc (DBA and SBA) and GlcNAc (WGA)residues but sialic acid was absent. Intracytoplasmic vesicles (Fig. lc,d) were very heavily stained with Con A (MadGlc) and WGA (GlcNAc) lectins. In contrast, lysosomes sparsely showed Man/Glc (Con A) residues. Concerning the zona pellucida (Fig. 2) we were able to detect a very heavy reaction with WGA lectin, as well as a heavy reaction with DBA (GalNAc), SBA (GalNAc), MPA (Gal), and PNA (Gal) lectins. Blasfocyst When the embryo reached the blastocyst stage the lectin labelling shown by the two cell populations, trophectodermal cells and inner cell mass, was separately evaluated. The lectin binding profile of the blastocyst zona pellucida (Fig. 2) is similar to the patterns shown during cleavage stages: very heavy staining for WGA, light for MPA and PNA and sparse for DBA and GS-11. Only SBA and DBA lectins decreased their reactions in this phase, which means that GalNAc residues were lost. Trophectoderm The outer cell surface of the trophectodermal cells appeared remarkably marked with WGA and LcH lectins (Fig. 3a,c) and lightly stained by SBA (Fig. 3b), MPA and PNA lectins (Fig. 3d). However, the reaction shown by DBA, LPA (Fig. 3e), and UEA-I lectins was sparse. A poor labelling of the intercellular zones was evidenced except for Con A and UEA-1 lectins. The reaction to lectins of the free cell surface of the ovine blastocyst varied qualitatively and quantitatively with respect to cleavage period. There was a n increase in staining for LcH, SBA, WGA, MPA, and PNA and new binding sites for UEA-I and LPA. Concerning the intercellular membrane, there was a specific loss of staining due to the lack of DBA and to the decrease of SBA and WGA staining. P. DE PAZ ET AL. 540 Fig. 1. Sections of cleavage-stages sheep embryos. a: DBA lectin. A light staining of intercellular surfaces is shown ( x 84,000). b: WGA lectin. The intercellular membrane area shows a light labelling ( x 84,000). c: ConA lectin. A specific staining is observed in intracytoplasmic vesicles from the 8 cell embryo ( X 84,000). d WGA lectin. Intracytoplasmic vesicles with a heavy labelling ( X 84,000). A heavy reaction to the Con A lectin (Fig. 3f) and a light one to LcH and WGA lectins was found in the intracytoplasmic vesicles from ovine blastulae. Lysosomes were also very heavily marked by WGA (Fig. 3g), lightly by Con A (Fig. 3h), and sparsely by LcH. The specific labelling pattern shown by cell organelles at the blastula stage was quite different. Intracytoplasmic vesicles globally reduced their staining with WGA with respect to cleavage stages. In contrast, lysosomes increased their staining with WGA and LcH lectins. Inner Cell Mass There is no specific polarity in the cell membrane of the blastocyst internal mass. This cell membrane showed a sparse histochemical reaction against the colloidal gold-conjugated lectins used in this study (Fig. 4a). The intensity of reaction showed by lysosomes varied in WGA (very heavy), LcH (light), and Con A (sparse). Nevertheless the intracytoplasmic vesicles reacted sparsely with LcH and WGA (Fig. 4b,c) and remarkably with Con A (Fig. 4d). Like the trophectodermal cells, the intracytoplasmic vesicles and the lysosomes from ICM blastomeres showed qualitative and quantitative differences with respect to the cleavage period. DISCUSSION The results obtained in the present work demonstrate a specific pattern in the distribution of sugar residues in the preimplantation ovine embryo. During ovine embryo cleavage the zanes that showed most sugar residues detected by colloidal gold conjugated lectins were the zona pellucida (GlcNAc), the intracytoplasmic vesicles (Man/Glc and GlcNAc), and the intercellular contact surface (GlcNAc and GalNAc). In contrast, a t the blastocyst stage, lectin labelling reduced in the intercellular surfaces but sugar residues increased on the free cell membrane (ManiGlc, GlcNAc, and Gal) and lysosomes (GlcNAc). The zona pellucida presents abundant GalNAc, GlcNAc, and Gal residues during preimplantation ovine embryo, but a t the blastocyst stage GalNAc residues are less abundant. LcH and Con A lectins, with Man/Glc affinity mainly bound to free cell surface in the trophoblast cells and to vesicles in the different stages of early ovine develop- LECTIN RECEPTORS IN EARLY OVINE EMBRYO 54 1 Fig. 2. Zona pellucida. a: WGA lectin. A very heavy labelling is observed ( X 24,000). b: DBA lectin 84,000). c: MPA lectin ( x 84,000). d PNA lectin ( x 84,000). (x ment. The changes in the Con A distribution patterns zones. The presence of SBA in the basal membrane of of the cell surface of the preimplantation embryo have the ectoderm and other epithelial structures has been already been described in mouse (Wu, 1980; Chavez, reported in the 7-day mouse embryo (Herken et al., 1990) and rabbit (Chen and Cao, 1992). Handyside 1990). Kimber and Bird (1985) have located DBA re(1980) has experimentally shown a polarization of the ceptors in 8 cell mouse embryos (compaction phase) and Con A binding sites in isolated blastomeres during mu- suggested a relationship between those receptors and rine embryo compaction. This author suggests that the compaction phase. In the same way we can relate such a distribution pattern of Con A receptors could cleavage and the abundant DBA staining observed in provide a molecular mechanism for attaining spatial the ovine intercellular surface. differentiation of trophectoderm and inner cell mass The lectins with high affinity to galactose (MPA and within the blastocyst. Kimber and Bird (1985) have PNA) show a sparse binding pattern throughout the shown that the recovery of the cell surface glycoconju- preimplantation period, except in the trophectodermal gates in the 8-16 cell embryo after enzymatic dissoci- microvilli and zona pellucida. In this sense, Handyside ation occurs at the same time a s recompaction, so these (1980) has described PNA receptors in murine morulae glycoconjugates may be involved in this process. This with a sparse staining a t the early stages, which inobservation agrees with the quantitative polarization creased a t the 16 cell stage after compaction. Rober and in the Man/Glc (LcH and Con A lectins) we found in the Holtz (1988) located galactose and fucose on the emintercellular surface of the trophoblast cells during bryonic membranes during pig embryo implantation ovine embryo cleavage. which disappear once the materno-fetal contact is esDBA and SBA staining patterns indicate a sparse tablished. D-galactose residues would play a role in the density of GalNAc residues in the free cell membrane implantation process as demonstrated by glycosylglycoconjugates of the blastomeres during cleavage, transferase treatments (Chavez, 1990). The exclusive but in the intercellular contact zones that density is presence of galactose residues at the free membrane higher. At the blastocyst stages, the free cell surface trophoblastic cells agrees with this hypothetic role. was richer in GalNAc residues than intercellular The absence of Lotus staining and the poor density of Fig. 3 LECTIN RECEPTORS IN EARLY OVINE EMBRYO 543 Fig. 4. Inner cell mass of the sheep blastocyst. a: WGA lectin. Lectin receptors are observed in the intercellular membrane ( x 84,000). b LcH lectin. Intracytoplasmic vesicles and lysosomes showing gold colloidal particles ( x 63.000). c: ConA lectin. Secondary lysosome ( x 84,000). d WGA lectin. Lysosome ( x 84,000). colloidal gold UEA-conjugated particles indicates the lack of fucosyl residues in the ovine preimplantation embryo. In contrast, Kimber and Bird (1985) and Dealtry and Sellens (1987) described the presence of receptors to fucose specific lectins in the preimplantation mouse embryo. Likewise, during preimplantation embryonic development we have only sparsely detected sialic acid residues (LPA) in the microvilli of the blastocyst trophectodermal cells. Chavez (1990) reported the presence of sialic acid residues on the trophoblast in the mouse. According to this author a loss of these residues is necessary to accomplish implantation. While GS-I1 receptors were not detected in the ovine Fig. 3. Trophectoderm of the sheep blastocyst. a: LcH lectin. Detail of the cell surface microvilli ( x 84,000).b: SBA lectin. Free surface of the blastomeres shows numerous microvilli i x 84,000).c: WGA lectin. Gold colloidal particles in the microvilli ( x 84,000). d: MPA lectin. Sparse staining in cell membrane ( x 84,000).e: LPA lectin. Cell membrane ( x 84,000). f:ConA lectin. Intracytoplasmic vesicles i x 84,000). g: lectin WGA. Very heavy labelling in a lysosome ( x 63,000). h ConA lectin. Secondary lysosome ( x 84,000). embryo during the stages analyzed, WGA shows the highest staining in our panel of lectin staining, indicating a greater distribution of GlcNAc residues during these development phases. These wide differences in the staining with GS-I1 and WGA lectins (both lectins have the same sugar affinity) may be due to the fact that GS-I1 lectin has high affinity and interacts with a non-reducing terminal GlcNAc group, but WGA has wider binding sites where 2-6 sugar residues can fit (Goldstein and Poretz, 1986). In contrast to our results, Kimber and Bird (1985) located BSL-I1 (GS-11) receptors in the preimplantation mouse embryo. Cell membranes from preimplantation ovine embryo show abundant GlcNAc residues, specially in the microvilli of the trophoblastic cells. Intracytoplasmic vesicles during cleavage and lysosomes in the zonal blastocyst also show abundant GlcNAc residues. The intracytoplasmic vesicles from mammal embryos do not have a definite function. An energy reserve function and a role in the cavitation process within the blastula have been proposed in other models (Mohr and Trounson, 1982) for embryos other than ovine. In the present work, we found a significant cy- 544 P. DE PAZ ET AL. tochemical identity of vesicles and lysosomes during preimplantation development which demonstrated these vesicles could be related to lysosomes in a n intracellular cycle. In this sense, Hyttel et al. (1988) suggest that the intracytoplasmic vesicles from bovine fertilized oocytes are involved in the formation of metabolic cell complexes. With respect to the participation of these organelles in the cavitation process, the intracytoplasmic vesicles of morula cell are heavily stained for ConA (MadGlc) a n WGA (GlcNac) but i t is sparse in the intercellular of trophectodermal cells. This could mean that intracytoplasmic vesicles do not suffer exocytosis during the cavitation process or the sugar residues of glicoproteins are modified, since no membranes appear with such a definite staining in the blastocoele. The abundant labelling of the ovine zona pellucida for Gal, GalNac, and GlcNac sugars during the preimplantation period is in agreement with the observations made by several authors (Kan et al., 1990; Shalghi et al., 1991; Miller et al., 1993). In this sense, it is highly significant that the zona pellucida is a distinctive extracellular matrix to which have been attributed the functions of species specificity of fertilization, block to polyspermy, and protection of the embryo during early development (Riddell et al., 1992). Boice et al. (1992) indicated that the bovine oviduct-specific secretory glycoconjugates become intimately associated with early cleavage stage embryos, thus possibly playing a critical role in normal embryonic development. The differences in sugar composition described in the ovine preimplantation embryo should reflect molecular characteristics whose developmental variations may have a functional significance. In the present work we detect a regional polarization of ManIGlc (Con A and LcH), GalNAc (DBA and SBA), and GlcNAc (WGA) residues in the intercellular contact zone of the blastomeres during cleavage when they are compared with the cell free surface. In contrast, the increase in the trophoblast cell membrane of some carbohydrates previously identified during cleavage (Man/Glc, GlcNAc, and Gal) is evidence of cell surface modifications, and so they could play a basic role during the preimplantation period. ACKNOWLEDGMENTS This work was supported by the CICYT grant GAN88-0102. LITERATURE CITED Bayna, E.M., J.H. Shaper, and B.D. Shur 1988 Temporally specific involvement of cell surface p-1,4 galactosyltransferase during mouse embryo morula compaction. Cell, 53t145-157. Boice, M.L., P.A. Mavrogianis, C.N. Murphy, H.G. Verhage, R.S. Prather, and B.N. Day 1992 Immunocytochemical analysis of the association of bovine oviduct. specific secretory glycoconjugates with early embryos. Theriogenology, 7t194. Brandley, B.K. 1991 Cell surface carbohydrates in cell adhesion. Semin. Cell Biol., 2t281-287. Bukers, A,, J. Friedrich, B.P. Nalbach, and H. Denker 1990 Changes in lectin binding patterns in rabbit endometrium during pseudopregnancy, early pregnancy and implantation. In: Trophoblast Research, Val. 4. Plenum Publishing, New York, pp. 285305. Chavez, D.J. 1990 Possible involvement of D-galactose in the implantation process. In: Trophoblast Research, Val. 4. Plenum Publishing, New York, pp. 259-272. Chen, S.Z., and Y. Cao 1992 Variations of lectin-binding glycoproteins in early pregnant rabbit embryo. Sci. China 35t577-584. Dealtry, G.B., and M.H. Sellens 1987 Lectin receptors on the peri- and early post-implantation mouse embryos. Roux Arch. Dev. Biol., 196:59-65. Fenderson, B.A., and E.M. Eddy 1990 Glycoconjugate expression during embryogenesis and its biological significance. Bioessays, 12: 173-179. Goldstein, I.J., and R.D. Poretz 1986 Isolation, physicochemical characterization and carbohydrate-binding specificity of lectins. In: The Lectin. Properties, Functions and Applications in Biology and Medicine. I.E. Liener, N. Sharon & I.J. Goldstein, ed. Academic Press, Orlando, FL, pp. 25-250. Gooi, H.C., T. Feizi, A. Kapadia, B.B. Knowles, D. Salter, and M.J. Evans 1981 Stage-specific embryonic antigen involves al-3 fucosylated type 2 blood group chains. Nature, 292t156-158. Handyside, A.H. 1980 Distribution of antibody- and lectin-binding sites on dissociated blastomeres fron mouse morulae: Evidence for polarization a t compaction. J. Embryol. Exp. Morphol., 60:99116. Hathaway, H.J., L.C. Romagnano, and B. Babiarz 1989 Analysis of cell surface galactosyltransferase activity during mouse trophectodermal differentiation. Dev. Biol., 134t351-361. Herken, R., B. Sander, and M. Hofmann 1990 Ultrastructure localization of WGA, RCA I, LFA and SBA binding sites in the sevenday-old mouse embryo. Histochemistry, 94.525-530. Hyttel, P., T. Greve, and N. Callesen 1988 Ultrastructure of in-vitro fertilization in superovulated cattle. J. Reprod. Fertil., 82t1-13. Kan FW, E. Roux, S. STJacques, and G. Bleau 1990 Demonstration by lectin-gold cytochemistry of transfer of glycoconjugates of oviductal origin to the zona pellucida of oocytes after ovulation in hamsters. Anat. Rec., 226:37-47. Kimber, S.J., and P.R. Bagley 1987 Cell-surface enrichment of fucosylated glycoconjugates in the 8- to 16-cell mouse embryo. An autoradiographic study. Roux Arch. Dev. Biol., 196t492-498. Kimber, S.J., and J.M. Bird 1985 Cell surface changes in preimplantation mouse embryos during compaction investigated using FITC conjugated lectins after proteolytic enzymes treatment. Roux Arch. Dev. Biol., 194t470-479. Lindner, G.M., and R.W. Wright 1983 Bovine embryo morphology and evaluation. Theriogenology, 20t407-416. Lis, H., and N. Sharon 1986 Lectins as molecules and as tools. Annu. Rev. Biochem., 55t35-67. Miller, C.C., R.K. Merkie, P.M. Brooks, A.B. Caudle, and R.A. FayrerHosken 1993 Carbohydrate and protein analysis of the equine zona pellucida. Theriogenology, 39t268. Mohr, L., and A.O. Trounson 1982 Comparative ultrastructure of hatched human, mouse and bovine blastocysts. J. Reprod. Fertil., 66:499 -504. Peacock, J.S., AS. Colsky, and V.B. Pinto 1990 Lectins and antibodies as tools for studying cellular interactions. J. Immunol. Methods, 126t147-157. Rastan, S., S.J. Thorpe, P. Scudder, S. Brown, H.C. Gooi, and T. Feizi 1985 Cell interactions in preimplantation embryos: Evidence for involvement of saccharides of the poly-N-acetyllactosamine series. J. Embryol. Exp. Morphol., 87:115-128. Riddell, K.P., D.A. Stringfellow, V.S. Panangala, and J.M. Scodras 1992 Monoclonal antibodies to the bovine zona pellucida. Theriogenology, 37r285. Rober, R.A., and W. Holtz 1988 Membrane-bound glycoconjugates and enzymes a t maternal-embryonal interaction sites during implantation in swine. In: 11th Int. Congr. Anim. Reprod. Artif. Insem., Dublin, vol. 2, pp. 120. Shalgi, R., R. Maymon, B. Barshir, D. Amihal, and E. Shutelsky 1991 Distribution of lectin receptor sites in the zona pellucide of folicullar and ovulated rat oocytes. Mol. Reprod. Dev., 29t365-373. Sharon, N., and H. Lis 1991 Lectins as cell recognition molecules. Science, 246t227-246. Shur, B.D. 1989 Glycoconjugates as mediators of cellular interactions during development. Curr. Opin. Cell Biol., 1:905-912. Spicer, S.S., and B.A. Schulte 1992 Diversity of cell glycoconjugates shown histochemically: A perspective. J. Histochem. Cytochem., 4Ot527-533. Watson, A.J. 1992 The cell biology of blastocyst development. Mol. Reprod. Dev., 33t492-504. Weibel, E.R. 1979 Stereological Methods. Val. 1.New York, Academic Press. Wiley, L.M. 1988 Trophectoderm: the first epithelium to develop in the mammalian embryo. Scanning Microsc., 2t417-426. Wu, J.T. 1980 Concanavalin A binding capacity of preimplantation mouse embryos. J. Reprod. Fertil., 58t455-461.


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May 8, 2019
San Pablo City Campus
Campus Wide
For all freshman passers, you are hereby advise to go to the respective college of your chosen course for an interview with the Dean/ Associate Dean/ Program coordinator/ and Faculty from May 8 to May 15, 2019 at exactly 9:00 in the morning up to 4:00 in the afternoon ONLY. Failure to appear on the said dates will mean that the waitlists will have their chance for ADMISSION INTERVIEW. Note: For Criminology Applicants, you will still have your PRACTICAL EXAMINATION on May 10 and May 16, 2019 ONLY. Your admission in...
December 6, 2017
San Pablo City Campus
Campus Wide
September 28, 2015 - ISO workshop was held at Siniloan Campus for the formulation of the University quality policy. This quality policy is the document jointly developed by LSPU management and experts to express the quality objectives of the University. This is a document that is well communicated and understood in the entire university system to underscore the specific duties of departments in ensuring quality. The LSPU Quality Policy: "We at LSPU are committed to provide quality, efficient and...
MARCH 8, 2016 Laguna State Polytechnic University (LSPU) President, Dr. Nestor M. De Vera signed academic partnership contract with Hanoi Metropolitan University (HNMU) Vice Rector, Hon. Hong Cuong Do from Vietnam at the Conference Room, LSPU-San Pablo City Campus, 9 A.M. The Memorandum of Agreement (MOA) includes the sending of 12 English professors from HNMU to LSPU-San Pablo on July 2016 for Teaching English Training. Among the Vietnamese professors to arrive for training is Hon. Phan Thi Pong Lan, Foreign Langu...
April 2, 2019
San Pablo City Campus
Campus Wide
Aliah Cyril Hernandez, a first year Accountancy student was declared Champion in the Level 1 competition of the 9th Paulo de Gala Macasaet Cup held on March 25, 2019 at San Pablo Colleges, San Pablo City, Laguna. The LSPU team represented by three first year students and six fourth year students garnered the 3rd highest accumulated points from all the three levels and was awarded as the 2nd Runner-up in the said competition. With its theme "Reign of the Accounting Wizards", the event was participated by...
October 28, 2019
San Pablo City Campus
College of Business Management and Accountancy
The Professional Regulation Commission (PRC) announces that 2,075 out of 14,492 passed the Certified Public Accountant Licensure Examination (CPALE) given by the Board of Accountancy in the cities of Manila, Baguio, Cagayan De Oro, Cebu, Davao, Iloilo, Legazpi, Lucena, Tacloban and Tuguegarao this October 2019. The passing rate for this October 21, 2019 is 14.32%-one of the lowest passing rates in recent years. In comparison, the October 2018 CPALE passing rate was 25.18%. ...


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Bettercare provides learning material to government and private hospitals and not-for-profit organisations in South Africa, Africa, and other developing countries in Asia and the Middle East.Read more
Bettercare provides learning material to government and private hospitals and not-for-profit organisations in South Africa, Africa, and other developing countries in Asia and the Middle East.Read more
Cancer Awareness Centre of Kenya (CACK) is a Community Organization (CBO) registered in Kenya, located at Tumaini House 3rd floor suite 309, Moi Avenue, Nairobi. Cancer Awareness Centre of Kenya is an initiative that emphasizes on preventive checkups that will help reduce cancer related risks.Read more
Tumaini is the Swahili word for hope. The Tumaini Project was established to provide free antiretroviral (ARVs) treatment to Kenyans living with HIV.Read more
The eQuality Health Bwindi scheme is an innovative program aimed at helping the people of the Bwindi area to access quality and affordable health services.Read more
Global Emergency Care Collaborative is a registered non-profit organization that provides comprehensive training programs in emergency care to local community members in limited-resource, rural areas. GECC currently works in rural Uganda, Kenya, and Cambodia.Read more
Riders for Health (Riders) is an international NGO and not-for-profit social enterprise that manages vehicles used for health care delivery by partner organisations in sub-Saharan Africa. We manage 1,300 vehicles across sub-Saharan Africa.Read more
The "HealthyLife" branded voucher pilot program subsidized client demand for treatment for sexually transmitted infections (STIs) through a network of health care providers in four districts around Mbarara in western Uganda.Read more
The Blue Star Programme (BSP), a social franchise networking private sector providers, was established in Bangladesh by the Social Marketing Company (SMC) in June 1998 in order to improve access to quality health care services.Read more
DKT Indonesia helps decrease infant and maternal mortality and lower HIV infection rates by training and working with midwives and increasing the use of contraception in Indonesia through a dynamic social marketing program called Andalan.Read more
HeartString is a string of color-coded beads that are used by traditional midwives to assess feral heart tones in resource constrained settings. This is a culturally appropriate and acceptable tool that enable the midwife to seek help early in emergency and improve outcomes for a mother and unbornRead more
In Malawi, government-supported community health workers—called health surveillance assistants (HSAs)—deliver a range of maternal, neonatal, and child health (MNCH) interventions in several community packages, each developed vertically and supported by a different department within the Ministry oRead more
Joining Hands Initiative is a DFATD-funded project that aims to improve MNCH services within the communities in Tanzania. The initiative will aid in establishing systems to refer patients for required services, improve community awareness and health practices as well as increase demand for MNCH andRead more
doctHERs is a novel healthcare marketplace that connects female doctors to millions of under served patients in real-time while leveraging online technology. doctHERs circumvents sociocultural barriers that restrict women to their homes, access to quality healthcare and inclusion in the profession.Read more
LifeNet International's (LN) conversion franchise model includes nurse training, management training, growth financing, and pharmaceutical supply to transform quality of care and increase range of services in primary healthcare facilities in Burundi, Uganda, and the DRC.Read more


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Statutory Rules 1994 No. 3681
Industrial Chemicals (Notification and Assessment) Regulations2 (Amendment)
I, The governor-general of the Commonwealth of Australia, acting with the advice of the Federal Executive Council, make the following Regulations under the Industrial Chemicals (Notification and Assessment) Act 1989.
Dated 25 October 1994.
By His Excellency's Command,
Assistant Minister for Industrial Relations
1. Amendment
1.1 The Industrial Chemicals (Notification and Assessment) Regulations are amended as set out in these Regulations.
[NOTE: These Regulations commence on gazettal: see Acts Interpretation Act 1901, s. 48.]
2. New regulations 12A, 12B and 12C
2.1 After regulation 12, insert:
Fees for assessment applications under section 55 of the Act
"12A. (1) The fee payable on an application under subsection 55 (1) of the Act is the amount worked out according to the formula:
n ´
n is the number of persons who make the application;
a is:
(a) in the case of an application relating to a chemical that is assessed by taking into account only 1 of the matters specified in paragraphs 32 (2) (c), (d), (e) and (f) of the Act—$30,000; or
(b) in any other case—$60,000;
t is the total number of persons who have applied to have the priority existing chemical assessed (including, in the case of a joint application, each joint applicant);
b is $2,500.
"(2) The fee payable on a late application under subsection 55 (2) of the Act is $3,000 plus the fee worked out by the formula in subregulation (1).
Calculation of fees for assessment applications
"12B. (1) Immediately after the end of the period of 28 days mentioned in subsection 55 (1) of the Act in relation to a priority existing chemical, the Director must calculate the amount of the fee payable for each application for assessment of that chemical.
"(2) If a person makes a late application for assessment of a priority existing chemical, the Director must, after receiving that late application:
(a) calculate the fee payable in respect of that application; and
(b) recalculate the fee payable for each application (if any) and late application (if any) already received for assessment of that chemical.
"(3) After the Director calculates or recalculates the fee payable for an application or a late application under subregulation (1) or (2), he or she must inform each applicant in writing of the fee so calculated or recalculated.
Payment of fees for assessment applications
"12C. (1) The fee payable for an application for assessment of a priority existing chemical under section 55 of the Act must be paid no later than 14 days after the day on which the Director informs the applicant under subregulation 12B (3).
"(2) If:
(a) a person pays an amount by way of fee for an application referred to in subregulation (1); and
(b) other applications are subsequently received for assessment of the same chemical and as a result the fee payable on each application for assessment of that chemical decreases;
the Director must, as soon as practicable after the payment, refund the difference to the person.".
3. Regulation 14 (Reduction of fees)
3.1 Omit the regulation, substitute:
Payment of other fees
"14. A fee prescribed under regulation 13 must be paid at the time of lodgment of the application, statement, nomination or notification to which it relates.".
4. Regulation 15 (Remission of fees)
4.1 Add at the end:
"(4) The Director may remit 10% of the fee paid in respect of an application under subsection 55 (1) or (2) of the Act if the information required to be given to the Director in relation to a chemical under subsection 58 (3) of the Act:
(a) is submitted at or before the end of the period specified in relation to the chemical under subsection 58 (4) of the Act; and
(b) is given in sufficient clarity and detail to allow proper assessment of the chemical.".
5. Regulation 16 (When must a fee be paid?)
5.1 Omit the regulation.
6. Schedule 2 (Fees)
6.1 Items 12 and 13:
Omit the items.
1. Notified in the Commonwealth of Australia Gazette on 1 November 1994.
2. Statutory Rules 1990 No. 231 as amended by 1990 No. 440; 1991 No. 165; 1992 Nos. 29, 245 and 282; 1993 No. 35; 1994 Nos. 21 and 320.


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Reference: OPP0006600
Activities: Outdoors, Practical, Regularly
Starts: Anytime
Grove Road, Stalybridge, SK15 3RL
We are currently looking for volunteers to assist our skilled Manchester operations team with the day-to-day maintenance of the canals in our area. No two days are the same – there will be a variety of tasks such as water control, reactive works and lock maintenance.
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If you're serious about making money selling things online, it's pretty much impossible to not recommend Shopify. The platform gives you everything you need to get your online store up and running in less than a day including a custom domain, beautiful templates (so you don't need design skills), secure payment options, and they can even take care of marketing and shipping for you. Whether you're promoting your own products, designs, or curating other products for people in your niche, Shopify is the best option for powering your online store.
Great article, I have tried a few over the years. I had a side business cleaning offices for years. It did great, but I hated never being able to take more than 3-4 days vacation, so I eventually gave up. I tried MTurk, but if you multiplied my earnings on an hourly basis, it was a little over $6/hr. My blog is much worse, probably less than $0.25/hr.
A second topic I really like is the fallacy of the great idea. You do not need some great, original idea to be successful. Really all you need to do is take an existing idea and put a new spin on it. Do it better than anyone else. Combine two existing ideas that have not been combined so far. Take a look at Mcdonalds. They do not make the best hamburger in the world. They are not even close. But they do the whole process better than anyone else. The idea is not important, the proper execution of the idea is where the money lies.
Start by taking other courses you're interested in: Not only is this important competitor and opportunity analysis, but it also gives you an idea of how a course could or should look and feel. What's the pacing like? Is it via email, video, in-person chats? Once you understand how you want your course to look, it's time to decide what it should include. Those same courses are a great starting place. How can you make your course better or more interesting? Do you have experience others don't?
You could easily do home organizing for people, an industry that has gained a lot of popularity since the debut of Netflix's hit series Tidying Up with Marie Kondo. If you're a tidy and organized person yourself, and you're good at organizing spaces, why not offer your services to people around you? You'd be surprised at how many people, even on your own social media feed, might take you up on doing something like this.
Billshark will negotiate with your current providers to lower your bills. They can work with your cable, wireless phone, satellite TV and radio, internet, and home security providers. Billshark is good at it too. They have an 85% success rate. According to Billshark, the average customer who gives them two bills to negotiate will save as much as $1,500!
Love dogs, but not ready to get one of your own? Get your fix by taking care of other people's pooches — and get paid for it. If your home isn't dog-friendly, consider becoming a dog walker. Apps like Wag! offer on-demand dog walking, so you can pick up walks when your schedule allows. If you have space (and your landlord's permission, if you rent), offer overnight dog boarding. Dog sitters on Rover.com, the go-to site for pet-sitting, generally command $25 to $35 a night, according to the company.
Take the phrase "one person's trash is another person's treasure" to heart by purging your closet of clothes that you don't wear anymore. You'll have the best luck selling items that are on trend, so consider purging items that are still in style, but you don't wear anymore. Head to a local thrift shop or consignment shop to see how much your clothes are worth!
Ask for a raise. If you're unhappy with your compensation at your 9-5 job, asking for a raise is one way to beef up your bank account. Most employers offer an annual review of your work – which could be the perfect time to negotiate a higher salary or ask for better perks. If your employer doesn't offer such an opportunity, it might be time to initiate a review yourself.
Rather than making money through subscriptions, YouTube channels are based on a traditional advertising system. Meaning the more viewers you get, the more you make. Once you're approved for the YouTube Partner Program and can start including ads on your videos, with every 1,000 views, you will make approximately $2-$4. Which might not seem like a lot, but if you have 100 videos with 5,000 views a month each, that would be $1,000–$2,000 already. Just imagine if your videos start hitting millions of views!
Extraordinary article! The most ideal approach to build your cash is to contribute the distinction between your costs and pay. Saving them in a financial balance won't help. I have been begun contributing since I was 21. I used to purchase a little measure of offers. In the end, I began putting resources into greater things like land and my online business. Most likely, there is dependably a hazard included yet the reward is basically high. So continue contributing. Begin with little and after that go for higher.
Developing the discipline it takes to pay yourself first is a process, and so it's helpful to use automation tools to help hold yourself accountable. You can set up automatic paycheck deductions for your 401(k) or IRA so that savings is automatically deducted. You can also use a savings platform or application to set up automatic savings contributions.
Great article, I have tried a few over the years. I had a side business cleaning offices for years. It did great, but I hated never being able to take more than 3-4 days vacation, so I eventually gave up. I tried MTurk, but if you multiplied my earnings on an hourly basis, it was a little over $6/hr. My blog is much worse, probably less than $0.25/hr.
If you've got expertise in a certain area, package up your knowledge into an online course and sell it. This has become a very popular business model for online entrepreneurs over the past several years. The two big websites that are used to sell online courses are Udemy and Teachable. Check out this awesome article by Regina on How to Create an Online Course that Sells.
We invite readers to respond with questions or comments. Comments may be held for moderation and will be published according to our comment policy. Comments are the opinions of their authors; they do not represent the views or opinions of Money Under 30. Comments have not been reviewed or approved by any advertiser, nor are they reviewed, approved, or endorsed by our partners. It is not our partner's responsibility to ensure all posts or questions are answered.
Go hunting or foraging for food. You may need to invest in gear and permits, but if you already have these, this is an inexpensive way to get your own food. If you're ethically against the killing of animals, it's pretty easy to forage for food, depending on where you live. Just make sure to forage only for food whose origin and properties you are sure of. Getting sick or poisoned is never any fun.
Tim, I know several millionaires, and most of them have done it the old fashioned way, which is working and saving over many years. Some have started their own businesses, some have made wealth through other means such as investments and real estate, and some have done it other ways. Virtually all of the millionaires I know have families (some were married without children, but most had children). I've never counted how many millionaires I know, but of the people I know for certain, I would venture to say that I know at least 10-15 of them would qualify under these standards. As for the college education part of it, I've never taken a poll to find out how many had degrees and which degree they had, if they had one.
Rent out a room on Airbnb. Living near a tourist area has its perks, including the prospect of renting out a room for a profit. With home sharing sites like Airbnb, you can rent out a room in your home – or even the entire place – for a day, a week, or longer. If you have extra space and might enjoy the company of travelers, renting out a room is great way to earn some extra money with little effort on your part. See our post, "How to Make Money as an AirBNB Host."
People are always looking to have their cars washed and detailed. You could be a mobile car washer and detailer without having a permanent location. Reach out to people you know or make some flyers and put it in your neighbors' mailboxes. If you want to get serious about it, prop up a one-page website or give out business cards. You can make money quickly doing this.
Leapforce is always looking for people for this work at home role. Their Search Engine Evaluators "conduct research, evaluation, and feedback on search engine results by measuring the relevance and usefulness of web pages in correlation to predefined queries, by providing a comparative analysis of sets of search engine results and various other techniques."
Not only will this multiply the money you're bringing in in a serious way, but it protects you against any sudden changes in the market or in your business. Remember that old saying about putting all your eggs in one basket? A few hours a week committed to just one or two of the following opportunities will put you in a much stronger position to be financially safe and independent.
More and more companies and startups especially are embracing remote work—where you use online collaboration and communication tools to do your work from wherever you want. And you don't have to be a 20-something hotshot designer or coder to reap the benefits of working remotely. Many remote positions are for customer support positions or other customer-facing positions that don't require specialized skill sets.
If this is the first time you are hearing about this, though, then we actually do not recommend trying it. The average skill level of players on the Internet has improved so much over the past few years that it's virtually guaranteed that a new player will lose money over the medium to long-term. But hey, Ben made upwards of 6 figures playing online in poker in college, so we had to list it. 🙂
Some companies like 1-800flowers.com outsource customer-service operations to third-party companies who then hire home-based workers or "agents" to take calls and orders. When you call 1-800-flowers, you may be speaking with Rebecca Dooley, a retired police officer and employee of Alpine Access, a major call-center service. When you dialed the number to 1-800-flowers, your call was automatically routed to Rebecca's spare bedroom in Colorado.
Another way to utilize your talent and business skills is to run corporate workshops online. Businesses are always looking for unique ways to help educate their workforce, and if you can package your talents into a day or half-day long session, you can sell that to companies all over the world to make money online. Start by building a portfolio and then reaching out on LinkedIn to influencers at relevant companies to see if they would be interested in you teaching their team.
Hey!, I'm just putting some feelers out there to see if there's anyone interested in making a pretty substantial amount of cash in a short amount of time. Only thing this requires is that you have an active bank account or credit card in the US. No cash is required up front to start. Which means your account can be on a zero balance and that's completely fine. email:[email protected] or whatsapp +1(781) 720 7259, lets talk about the next deal


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She looks so innocent and cute selling cookies in her little dress...gets fucked hard and takes a load on her adorable and pretty face (Asuna Fox)
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BB's first major turning point came in 1948 when he performed on Sonny Boy Williamson's radio show on KWEM in West Memphis. Disney Frozen Characters Christmas Tree shirt. This led to steady participation at Sixteenth Avenue Grill in West Memphis, and then to a ten-minute position on Memphis radio with WDIA black staff and management. Shortly after his number one hit, Three Three O'Clock Blues, BB began a nationwide tour. In 1956, BB and his band played an incredible night with 34 songs. From home town with small town cafes, pubs, and country halls to rock palaces, symphony concert halls, universities, resort hotels and theaters, in the country and internationally, BB has become the most famous blues musician for the past 40 years.
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"America is great because she is good. If America ceases to be good, America will cease to be great," wrote French political scientist Alexis de Tocqueville in his 1835 book, "Democracy in America". The USA, he believed, offered equality that was not seen in other nations. The founding fathers of Continue Reading
Can The President of the United States Go To Jail?
Deep state is weaponizing staff security clearances against Trump: Sean Bigley
How to Get Back the Middle Class in America
dine in seattle a done what's up all right uh… yet disregard that most talk about just moments before fred not mistaken the president has presidential powers therapeutically given to whom by the previous republican president to ordered execu orders trooper example partly force recovers handles filibustering cosmic gridlock cameras Continue Reading
Middle Class Decline – Reagan
that it's going to come as an incredible shot to most americans that were not the richest nation in the world on america likes to think of itself is the richest nation on the planet in space they considered common knowledge a which countries they register america cars three or Continue Reading
Westboro Baptist Church Interview On Anonymous Threat/Hoax, Calls Host "Little Girl"
Announcer: The David Pakman Show at www.DavidPakman.com. David: Welcome back to the show. It has been reported that the hacking group Anonymous, which was involved in Wikileaks sent a letter to the Westboro Baptist Church indicating that they would be targeting the Westboro Baptist Church. Westboro Baptist Church, of course Continue Reading
Voters' reaction to Trump's State of the Union
Why Is US Middle Class No Longer Richest?
joining us today is richard wolf is professor emeritus of economics at the University of Massachusetts also a visiting professor at The New School University in New York City professor wolff we talked about this New York Times article which outlined how in spite of the American middle class being Continue Reading
Crazy Graphs: Rich Vs Middle Class & Poor
republicans always made the claim that we have a rising tide lifts all boats trickle-down economics don't wait leap years that we're giving gigantic access to the rich by delegates we get the arrest our way to go on uh… i so that's that's what you say uh… well we Continue Reading
The "Big Lie" About America's Middle Class
but first it now are trying to sell it or are not going to write a seven-hour colours that when you look at the thirty-four richest countries in the world the organization economic cooperation of all the deal e_c_ countries and you look at the relative well of their middle Continue Reading


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Quicken Wp Web-site 2018
4 months ago Comments Off on Quicken Wp Web-site 2018
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PANO – Sugar cane prices in the Cuu Long River Delta have remained stable and high and brought about a profit of nearly VND 30 million for planters. Currently, Ba Tri district (Ben Tre province) owns about 650ha of sugar cane. The area was planned to be extended this crop by weaving cane in with rice fields. The new method is enabled by the irrigation scheme which set up an anti-salt dyke system along Ham Luong River of San Hiep, Tan Hung, An Duc, An Binh Tay, An Ngai Trung, An Ngai Tay communes. Sugar cane breeds to be planted are subject to the National Sugar Programme's ones which can give 85 tonnes of product per hectare such as VN-84-4130, K84-200, ROC-18. Translated by Hoang Anh … [Read more...] about Ben Tre extends sugar cane area
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Teenager Fati makes history as Barcelona knock Inter out of Champions League
International Teenager Fati makes history as Barcelona knock Inter out of Champions League The Saigon Times Daily Wednesday, Dec 11, 2019,16:24 (GMT+7) Teenager Fati makes history as Barcelona knock Inter out of Champions LeagueThe Saigon Times Daily Barcelona's Anssumane Fati celebrates scoring their second goal with teammates at San Siro, Milan, Italy December 10, 2019 – PHOTO: REUTERS BARCELONA (REUTERS) - Inter Milan crashed out of the Champions League on Tuesday after 17-year-old Ansu Fati became the youngest scorer in the competition's history by firing in a late winner to seal Barcelona's 2-1 victory at San Siro. Barca coach Ernesto Valverde rested Lionel Messi and several other key players for the trip to Milan, having already secured top spot in Group F, but Carles Perez put the Spaniards in front on his Champions League debut after just 23 minutes before Romelu Lukaku levelled on the stroke … [Read more...] about Teenager Fati makes history as Barcelona knock Inter out of Champions League
Vietnamese films earn big profits
A scene in Hai Phượng , a production by movie star and film producer Ngô Thanh Vân of HCM City. The action film earned high ticket sales, around VNĐ200 billion ($8.6 million), one month after its release in Việt Nam in March. Photo VAA Entertainment By Thu Anh HCM CTY Fourteen Vietnamese films earned around VNĐ715 billion (US$30.7) in ticket sales in the first five months of the year, a year-on-year increase of 40 per cent, according to CGV Cinemas Việt Nam. Private film companies in HCM City sunk large amounts of money into movies for release during the Tết (Lunar New Year) holiday, which began in January. Producer Nhất Trung's Cua Lại Vợ Bầu (Love Again) raked in more than VNĐ108.9 billion ($4.7 million) in ticket sales in three weeks beginning in late January. Nearly 1.3 million tickets were sold. A scene from movei Cua … [Read more...] about Vietnamese films earn big profits
Textile sector stuck at bottom of value chain
Nguyen Thi Xuan Thuy, director of the Ministry of Industry and Trade's Centre for Supporting Industrial Development, said at a recent forum that Vietnam's textile industry is still dependent on import of production inputs. The country plans to have 30,000-76,000 hectares under cotton crops in the 2015-2020 period, but had only 1,000 ha in 2017, and cotton production that year was only 1,000 tons against a target of 20,000-60,000 tons, she said. The country targets annual fabric production of one billion meters but there is no allocation of funds for it, and so most material has to be imported for production, Thuy noted. Vietnam imports half the raw material for production from China, and this means its textile products would not enjoy zero import tariffs under the trade pacts it has signed, she added. But local feedstock producers struggle to sell domestically. Vu Huy Dong, CEO of thread producer Dam San, said 90 percent of his output is exported to China. "Chinese … [Read more...] about Textile sector stuck at bottom of value chain
The latest case of child abuse came to light earlier this week when CCTV footage in a Saigon elevator showed a man hugging a little girl Monday night. The man was quickly identified as Nguyen Huu Linh, former deputy chief prosecutor of Da Nang, who retired in 2018. Once the footage was leaked on the Internet, public outrage followed, and Linh had to face the music. People threw dirt at his house and someone painted au dam (paedophilia) in black paint on his gate. People threw dirt at his house and someone painted au dam (paedophilia) in black paint on his gate. Photo by VnExpress/N.T Angry comments filled social media, mincing no words. "A new kind of pervert has appeared in apartment building elevators, so parents should be cautious and always stay by their daughters to protect them from these perverts. Disgusting!," a VnExpress reader commented. "He knows the law and was once in a high position in the executive body of a major city, but he violated the law; there … [Read more...] about Vietnam shocked by extent of sexual abuse children face


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Assistant Professor, Departments of Statistics and Psychology
Clinical Science;
Education/Degree: Ph.D., Columbia University, 2013 About: Dr. Xu's research interest lies in the development of statistical methodology with its applications in psychological and educational measurement. Dr. Xu has been working on statistics and psychometrics problems in latent variable modeling, item response theory, cognitive diagnosis models, and computerized adaptive testing.
Website: https://sites.google.com/umich.edu/gongjunxu


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The new Sony Xperia smart phone allows users to take pictures which can then be 3D printed. Click here to see website.
Source: www.sonymobile.com
Photo Credit: Sony Mobile
Source: www.sonymobile.com
Photo Credit: Sony Mobile
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Here is a list of 3D printers that were mostly used during the second quarter of this year. Click...
This unique gel can transform based on temperature changes and could possibly be used for organ transplants eventually. Click...
Scientists working at SHRI(Sydney Heart Research Institute) have created cells that move and act as if they are alive....
Shining 3D has been around for over a decade and offers numerous 3D printers, 3D scanners and many other...
Sintavia announced last week that it will be making parts for Honeywell through it's additive manufacturing process. Click here...
This recent science report shows how 3D printing with live cells could be a way to repair damaged tissue....
The Mag ICreatum 3D printer allows users to not only 3D print, but also carve and laser engrave. Click...
The Quebec government is investing $3 million CAD into a 3D printer medical center. Click here to read the...
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