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Growth continues for online advertising underpinned by strong video trading
Although growth is slower than in previous years, digital has bucked the overall advertising slowdown, with the latest IAB Online Advertising Expenditure Report prepared by PwC revealing the market grew 3% quarter on quarter and 6.5% year on year to reach $2.381b for the September quarter.
According to eMarketer, the global e-commerce market is forecasted to grow 21% in 2019 to $3.54 trillion. The Asia-Pacific (APAC) region alone will account for 64% of total e-commerce sales worldwide this year, a 25% Year-on-Year (YoY) growth.
For brands in Southeast Asia (SEA), showing up in users' search results isn't just about driving clicks and conversions. Here, Jenny Lim, Search Performance Solution Specialist at Google Malaysia, breaks down our latest research with Ipsos and Kantar which shows the direct impact on awareness, recall, and consideration that being present in upper-funnel searches can have on brands.
Viewability has traditionally been the most popular indicator of a digital campaign's success. But just because an ad has a chance to be seen, does that mean that consumers are actually seeing it? Or remembering it?
IAB Australia's November 2019 Newsletter.
29th October 2019: Digital channels are effective at delivering brand impact and more cost efficient than most offline channels according to an Australian first report released today by Kantar at IAB Australia's MeasureUp conference. The report, The Digital Brand Effect, found that the long term retained brand impacts for digital campaigns are at least on par with those of other media; with 20% of the original brand impact of digital campaigns retained for eight weeks after the original exposure.
It is essential for our industry to create common language across the different platforms, so we have tasked our IAB Councils with updating a series of glossaries originally published in August 2017, and are adding in the new Digital Out Of Home Glossary.
Building on the foundation laid by the 2019 Dynata Global Trends Report, this spotlight report will explore three key themes: mobile, interconnectivity, and voice. These three factors are fundamental to how technology shapes the present and will shape the future.
Daily Mail Australia partnered with qualitative research agency The Lab Strategy, to speak to a panel of expert academics in the youth field.


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'Faithful' in the Bible
When the time drew near for him to die, he called his son Joseph and said to him, "If I have found favor in your eyes, put your hand under my thigh and promise me that you will deal with me in kindness and faithfulness. Do not bury me in Egypt.
You will lead the peopleYou have redeemedwith Your faithful love;You will guide them to Your holy dwellingwith Your strength.
6 but showing faithful love to a thousand generations of those who love Me and keep My commands.
Then the Lord passed in front of him and proclaimed:Yahweh—Yahweh is a compassionate and gracious God, slow to anger and rich in faithful love and truth,
maintaining faithful love to a thousand generations, forgiving wrongdoing, rebellion, and sin. But He will not leave the guilty unpunished, bringing the consequences of the fathers' wrongdoing on the children and grandchildren to the third and fourth generation.
Not so with My servant Moses;he is faithful in all My household.
The Lord is slow to anger and rich in faithful love, forgiving wrongdoing and rebellion. But He will not leave the guilty unpunished, bringing the consequences of the fathers' wrongdoing on the children to the third and fourth generation.
Please pardon the wrongdoing of this people, in keeping with the greatness of Your faithful love, just as You have forgiven them from Egypt until now."
But you who have remained faithful to the Lord your God are all alive today.
10 but showing faithful love to a thousand generations of those who love Me and keep My commands.
Know that Yahweh your God is God, the faithful God who keeps His gracious covenant loyalty for a thousand generations with those who love Him and keep His commands.
You are to fear Yahweh your God and worship Him. Remain faithful to Him and take oaths in His name.
For if you carefully observe every one of these commands I am giving you to follow—to love the Lord your God, walk in all His ways, and remain faithful to Him—
You must follow the Lord your God and fear Him. You must keep His commands and listen to His voice; you must worship Him and remain faithful to Him.
love the Lord your God, obey Him, and remain faithful to Him. For He is your life, and He will prolong your life in the land the Lord swore to give to your fathers Abraham, Isaac, and Jacob."
The Rock—His work is perfect;all His ways are entirely just.A faithful God, without prejudice,He is righteous and true.
He said about Levi:Your Thummim and Urim belong to Your faithful one;You tested him at Massahand contended with him at the waters of Meribah.
Only carefully obey the command and instruction that Moses the Lord's servant gave you: to love the Lord your God, walk in all His ways, keep His commands, remain faithful to Him, and serve Him with all your heart and all your soul."
Instead, remain faithful to the Lord your God, as you have done to this day.
She said to them, "Each of you go back to your mother's home. May the Lord show faithful love to you as you have shown to the dead and to me.
He guards the steps of His faithful ones,but the wicked perish in darkness,for a man does not prevail by his own strength.
"'Then I will raise up a faithful priest for Myself. He will do whatever is in My heart and mind. I will establish a lasting dynasty for him, and he will walk before My anointed one for all time.
If I continue to live, treat me with the Lord's faithful love, but if I die,
don't ever withdraw your faithful love from my household—not even when the Lord cuts off every one of David's enemies from the face of the earth."
Ahimelech replied to the king: "Who among all your servants is as faithful as David? He is the king's son-in-law, captain of your bodyguard, and honored in your house.
But My faithful love will never leave him as I removed it from Saul; I removed him from your way.
I am a peaceful person, one of the faithful in Israel, but you're trying to destroy a city that is like a mother in Israel. Why would you devour the Lord's inheritance?"
With the faithfulYou prove Yourself faithful;with the blameless manYou prove Yourself blameless;
And Solomon replied, "You have shown great and faithful love to Your servant, my father David, because he walked before You in faithfulness, righteousness, and integrity. You have continued this great and faithful love for him by giving him a son to sit on his throne, as it is today.
Give thanks to the Lord, for He is good;His faithful love endures forever.
With them were Heman, Jeduthun, and the rest who were chosen and designated by name to give thanks to the Lord—for His faithful love endures forever.
I will be a father to him, and he will be a son to Me. I will not take away My faithful love from him as I took it from the one who was before you.
And Solomon said to God: "You have shown great and faithful love to my father David, and You have made me king in his place.
The trumpeters and singers joined together to praise and thank the Lord with one voice. They raised their voices, accompanied by trumpets, cymbals, and musical instruments, in praise to the Lord:For He is good;His faithful love endures forever.The temple, the Lord's temple, was filled with a cloud.
All the Israelites were watching when the fire descended and the glory of the Lord came on the temple. They bowed down on the pavement with their faces to the ground. They worshiped and praised the Lord:For He is good,for His faithful love endures forever.
The priests and the Levites were standing at their stations. The Levites had the musical instruments of the Lord, which King David had made to praise the Lord—"for His faithful love endures forever"—when he offered praise with them. Across from the Levites, the priests were blowing trumpets, and all the people were standing.
Then he consulted with the people and appointed some to sing for the Lord and some to praise the splendor of His holiness. When they went out in front of the armed forces, they kept singing:Give thanks to the Lord,for His faithful love endures forever.
After these faithful deeds, Sennacherib king of Assyria came and entered Judah. He laid siege to the fortified cities and intended to break into them.
As for the rest of the events of Hezekiah's reign and his deeds of faithful love, note that they are written in the Visions of the Prophet Isaiah son of Amoz, and in the Book of the Kings of Judah and Israel.
The rest of the events of Josiah's reign, along with his deeds of faithful love according to what is written in the law of the Lord,
They sang with praise and thanksgiving to the Lord: "For He is good; His faithful love to Israel endures forever." Then all the people gave a great shout of praise to the Lord because the foundation of the Lord's house had been laid.
Then I put my brother Hanani in charge of Jerusalem, along with Hananiah, commander of the fortress, because he was a faithful man who feared God more than most.
You found his heart faithful in Your sight,and made a covenant with himto give the land of the Canaanites,Hittites, Amorites, Perizzites,Jebusites, and Girgashites—to give it to his descendants.You have kept Your promise,for You are righteous.
They refused to listenand did not remember Your wondersYou performed among them.They became stiff-necked and appointed a leaderto return to their slavery in Egypt.But You are a forgiving God,gracious and compassionate,slow to anger and rich in faithful love,and You did not abandon them.
Then I instructed the Levites to purify themselves and guard the gates in order to keep the Sabbath day holy.Remember me for this also, my God, and look on me with compassion in keeping with Your abundant, faithful love.
You gave me life and faithful love,and Your care has guarded my life.
He causes this to happen for punishment,for His land, or for His faithful love.
Know that the Lord has set apartthe faithful for Himself;the Lord will hear when I call to Him.
But I enter Your houseby the abundance of Your faithful love;I bow down toward Your holy templein reverential awe of You.
Turn, Lord! Rescue me;save me because of Your faithful love.
For the choir director: according to Sheminith. A Davidic psalm.Help, Lord, for no faithful one remains;the loyal have disappeared from the human race.
But I have trusted in Your faithful love;my heart will rejoice in Your deliverance.
For You will not abandon me to Sheol;You will not allow Your Faithful One to see decay.
Display the wonders of Your faithful love,Savior of all who seek refugefrom those who rebel against Your right hand.
With the faithfulYou prove Yourself faithful;with the blameless manYou prove Yourself blameless;
For the king relies on the Lord;through the faithful love of the Most Highhe is not shaken.
Only goodness and faithful love will pursue meall the days of my life,and I will dwell in the house of the Lordas long as I live.
Remember, Lord, Your compassionand Your faithful love,for they have existed from antiquity.
Do not remember the sins of my youthor my acts of rebellion;in keeping with Your faithful love, remember mebecause of Your goodness, Lord.
All the Lord's ways show faithful love and truthto those who keep His covenant and decrees.
For Your faithful love is before my eyes,and I live by Your truth.
Sing to Yahweh, you His faithful ones,and praise His holy name.
I will rejoice and be glad in Your faithful lovebecause You have seen my affliction.You have known the troubles of my life
Show Your favor to Your servant;save me by Your faithful love.
May the Lord be praised,for He has wonderfully shown His faithful love to mein a city under siege.
Love the Lord, all His faithful ones.The Lord protects the loyal,but fully repays the arrogant.
Therefore let everyone who is faithful pray to Youat a time that You may be found.When great floodwaters come,they will not reach him.
Many pains come to the wicked,but the one who trusts in the Lordwill have faithful love surrounding him.
Now the eye of the Lord is on those who fear Him—those who depend on His faithful love
May Your faithful love rest on us, Yahweh,for we put our hope in You.
Lord, Your faithful love reaches to heaven,Your faithfulness to the clouds.
God, Your faithful love is so valuablethat people take refuge in the shadow of Your wings.
Spread Your faithful love over those who know You,and Your righteousness over the upright in heart.
For the Lord loves justiceand will not abandon His faithful ones.They are kept safe forever,but the children of the wicked will be destroyed.
The Lord will send His faithful love by day;His song will be with me in the night—a prayer to the God of my life.
Rise up! Help us!Redeem us because of Your faithful love.
God, within Your temple,we contemplate Your faithful love.
"Gather My faithful ones to Me,those who made a covenant with Me by sacrifice."
For the choir director. A Davidic psalm, when Nathan the prophet came to him after he had gone to Bathsheba.Be gracious to me, God,according to Your faithful love;according to Your abundant compassion,blot out my rebellion.
For the choir director. A Davidic Maskil. When Doeg the Edomite went and reported to Saul, telling him, "David went to Ahimelech's house."Why brag about evil, you hero!God's faithful love is constant.
But I am like a flourishing olive treein the house of God;I trust in God's faithful love forever and ever.
I will praise You forever for what You have done.In the presence of Your faithful people,I will put my hope in Your name, for it is good.
He reaches down from heaven and saves me,challenging the one who tramples me.SelahGod sends His faithful love and truth.
For Your faithful love is as high as the heavens;Your faithfulness reaches the clouds.
My faithful God will come to meet me;God will let me look down on my adversaries.
But I will sing of Your strengthand will joyfully proclaimYour faithful love in the morning.For You have been a stronghold for me,a refuge in my day of trouble.
To You, my strength, I sing praises,because God is my stronghold—my faithful God.
May he sit enthroned before God forever;appoint faithful love and truth to guard him.
and faithful love belongs to You, Lord.For You repay each according to his works.
My lips will glorify Youbecause Your faithful love is better than life.
May God be praised!He has not turned away my prayeror turned His faithful love from me.
But as for me, Lord,my prayer to You is for a time of favor.In Your abundant, faithful love, God,answer me with Your sure salvation.
Answer me, Lord,for Your faithful love is good;in keeping with Your great compassion,turn to me.
Has His faithful love ceased forever?Is His promise at an end for all generations?
Then they would not be like their fathers,a stubborn and rebellious generation,a generation whose heart was not loyaland whose spirit was not faithful to God.
Show us Your faithful love, Lord,and give us Your salvation.
Faithful love and truth will join together;righteousness and peace will embrace.
Protect my life, for I am faithful.You are my God; save Your servant who trusts in You.
For You, Lord, are kind and ready to forgive,rich in faithful love to all who call on You.
For Your faithful love for me is great,and You deliver my life from the depths of Sheol.
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- Faithfulness, In Human Relationships
- Faithfulness, To God
- God Showed His Lovingkindness
- God Shows His Lovingkindness
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We have done AKL for several years and will be doing a more budget-friendly trip in January 2014. We've tried to read as much about POP as possible, but are having a bit of trouble with deciding which building/room requests we should try to make. It's a week long trip in mid-January starting MLK weekend, and we are experienced Disney people.
Any insights and/or suggestions would be most welcome as we prepare to book our trip.
These are our priorities as far a a room is concerned:
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*We've read that building 3 and 9 are popular as far as the buses/main building is concerned, but parking lot views seem a bit un-Disney-like... What buildings, floor levels, and facing preferences do any of you recommend?


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A home business can help you to make money and adds extra income. You must make sure to turn a profit, and this can be hard.What needs to be done if you think it takes to run and start a successful work from home business? This article is going to go over what needs to be done for starting a strong online business.
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Don't get sucked into the activities that you may find around your home, though take breaks during your working hours. Don't start long home improvement projects or playing a video game.
Have a page on your site. This will enable you to swap banner links with other businesses that you know. This is a great way to increase your rankings and make sure customers can find you.
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Man fatally injured when knocked off top of viaduct
WAHOO, Neb. (AP) - Authorities say a man was fatally injured when he was knocked off the top of a viaduct in eastern Nebraska.
Saunders County Sheriff Kevin Stukenholtz said in a news release Friday that several vehicles were involved in pileup around 6:20 p.m. Thursday on the U.S. Highway 77 viaduct on the west side of Wahoo.
The release says one man had left his vehicle to check on another driver when a northbound semitrailer went out of control on the icy pavement and struck the viaduct.
The man who was knocked off it was taken to Saunders Medical Center in Wahoo and then to a Lincoln hospital, where he was pronounced dead.
His name hasn't been released.
The sheriff says no other injuries were reported by people in the other vehicles.
Copyright 2019 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


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Provides pull-out tracings for five floral paintings, along with instructions for how to bring them to life with watercolour paint. This book offers guidance on how to transfer the tracings to watercolour paper, along with a section on what materials to use.
Learn how to paint 40 exquisite flower portraits in watercolour following Billy Showell's step-by-step instructions.
Learn to paint beautiful watercolor flowers in simple steps with this free and easy approach to watercolor painting for beginners. Marie Boudon's beautifully presented creative course will give you a good grounding in this new-to-you medium and teach you all you need to know to get started with painting flowers in watercolor.
Jean Haines' distinctive, expressive style is influenced by the places she's lived in and the people she's met during her life, and in this beautiful, inspiring book she takes you on an exciting journey through her world of watercolour painting.
The 30 Minute Artist series is for beginners and busy artists who want to achieve great paintings in just half an hour.
Jean Haines' enthusiasm for watercolours burst through every page of this inspirational book that will guide you, step by step, to creating beautiful and unique paintings of your own, whatever your ability.
In Color Harmony for Artists, you'll learn to create expressive palettes and paintings through artist Ana Victoria Calderon's stunning work and inspiring instruction.
Anna's clear teaching will give you confidence in painting beautiful watercolour flowers in a modern twist on the botanical style, From complete beginners to more experienced watercolourists, this book offers in-depth instruction on drawing and painting, plus dozens of inspirational floral artworks.
A clear, comprehensive guide to colour mixing for the watercolour artist. It begins with simple colour theory and a colour wheel, with a template provided so that readers can paint their own; a vital step towards understanding colour.
'If there was ever a book you'd been eagerly awaiting, this is it. Subtitled "a lifetime of painting techniques", this absolutely lives up to expectations and won't disappoint.' Artbookreview.netAn invaluable source of tips and techniques based on a life-time of painting.
Fashion illustrator Veronica Ballart Lilja proves there's more to watercolour with a fresh and creative approach to this classic art form.
Jean Haines' first-hand experience of the energising and life-enhancing effects of painting makes her a perfect example of how watercolours can work for you.
'Search Press have reissued their eminently useful Top Tips series in paperback. If you haven't encountered these before, they're a handy way of taking the advice of your favourite artists out in the field with you.' Paint Magazine
Expert watercolour landscape painter, Geoff Kersey, explains how to work from photographic reference to produce beautiful paintings. Clear tips and advice are accompanied by Geoff's reference photographs, colour charts, preparatory sketches and finished paintings, and there are full details of the adaptations and creative processes involved.
This long-awaited follow up to Terry Harrison's Sea & Sky in Watercolour has six new projects and a wealth of beautiful new paintings.
Enter a world of ravishing light and color. English Romanticist J. M. W. Turner transformed the essence of landscape painting from serene contemplative scenes to pictures pulsating with life, as if the sun itself were gleaming out of his canvases. Discover the shimmering works and wide repertoire of the "father of light," who pit the Industrial...
Terry Harrison's clear, no-nonsense advice and easy to follow instructions make painting accessible for people who have never had the confidence to try before.
In this beautiful book, best-selling author Jean Haines shows you how to breathe life into your flower paintings, and experience the pleasure that can be gained from the art of painting flowers.
"First published in hardback by Search Press 2001; reprinted in paperback in 2005."--Title page verso.
'I have reviewed a lot of books on painting for beginners but this series is the best to date. Very highly recommended for anybody who wants to learn watercolour painting.' www.myshelf.comWith 3 colours, 3 brushes and this practical advice, you can start painting watercolour landscapes.
'Search Press have reissued their eminently useful Top Tips series in paperback. If you haven't encountered these before, they're a handy way of taking the advice of your favourite artists out in the field with you.' Paint Magazine
Take a masterclass with artist Matthew Palmer in this comprehensive guide to watercolour painting.
Be inspired to think outside your humble box of watercolours.
An inspiring and accessible guide to painting beautiful snow scenes using easy-to-master techniques.
Billy showell's exquisitely executed watercolour portraits of fruit and vegetables give an unconventional, often amusing, twist to traditional botanical studies, taking them on to a new and exciting contemporary level.
Learn to paint a variety of mountain scenes with Matthew Palmer using just three colours and three brushes.
This book is a highly practical step-by-step guide to working with gouache and not only teaches and inspires novice painters but also introduces more experienced artists to the advantages of using this much overlooked, but wonderful, medium.
A comprehensive guide to watercolour painting that draws on the expertise of seven well-loved artists and includes a wide variety of techniques and styles.
Forget everything you think you know about traditional watercolour painting - Paint Play will show how to experiment with paint, use it spontaneously and have fun, no experience required!
This is a unique hardback book, capturing Ashley's personal sketches of Yorkshire, opening up his private sketch book , this is an insight into his private thoughts and feelings captured through his watercolour sketches.
This is a book for all landscape artists whatever their skill levels, and anyone who wants to learn more about painting trees whether they are magnificent in their winter habitat, gloriously bedecked in spring foliage, splendid in their summer raiment or stunning when their leaves turn to russet reds and spun golds.
'This is one of the most original, entertaining and instructive books I've seen in a long time.' Artbookreview.netA portable course for budding watercolourists, built up subject by subject through over 50 paintings.
Practical tips and techniques to get you started in watercolour.
Skies, light and atmosphere are perfect subjects for watercolour, and David Bellamy's mastery of the medium shines out in this inspiring and practical book. There is clear explanation of the techniques needed to paint these vital elements of the landscape, and advice on the choices painters need to make in order to ensure success.
This stunning guide with original artworks by Emma Faull will enable painters of all abilities to create exquisite watercolour paintings of birds.
Take your watercolour painting to new heights with this exciting and inspirational book that will give you innovative ways to bring vibrancy, texture and dynamism into your watercolours art.
A stimulating mix of colour and watercolour technique for all skill levels, this guide helps the artist develop a free and flowing style to their art.
Just Add Watercolour offers creative painting tips and accessible advice, providing both practical information and inspiration.
Artist Ann Blockley is renowned for her innovative approach to watercolour painting. Her highly-anticipated new book provides a series of workshops demonstrating how to push the boundaries of watercolour, using key techniques to develop your own work and take your paintings to the next level.
Reintroduces the genius of an enigmatic scientist and observer of the natural world - Ernst Haeckel. While his drawings display an understanding of biological structure, the skill with which he drew these aquatic protozoa renders them genuine works of art. This book features commentary and descriptions of the radiolarians from Haeckel's work.
A new, up-to-date edition of the popular and comprehensive encyclopedia to watercolour painting by professional artist Hazel Harrison.


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The ingredient of Sea breeze
- 10 white sugar cubes
- 125ml (1/2 cup) vodka
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- 2 x 750ml btls chilled sparkling wine
The instruction how to make Sea breeze
- Place a sugar cube in each serving glass. Pour vodka evenly among glasses and set aside for 1 minute to soak.
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The Federation of Neighbourhood Associations of Barcelona (FAVB), an organisation combating the "touristification" of the Catalan capital, has recently come up with an unusual method to achieve its goal: instead of holding protests against local authorities' policies and assaulting buses with tourists, as some locals did during the summer of 2018, FAVB has started spreading leaflets among city visitors asking for a simple favour - "don't tell anyone that you have been in Barcelona".
La turistificació afecta la vida del veïnat. Amb l'acció #AmagaBarcelona, la @FAVBcn vol explicar als turistes que la ciutat necessita polítiques que fomentin el #DecreixementTurístic. #EscondeBarcelona😉— FAVB (@FAVBcn) 29 июля 2019 г.
🗓Dimarts 30 de juliol
⏰11:00 hores
📌 Marina (Portal del Naixement) pic.twitter.com/wBTohKplRR
To be precise, the organisation is asking tourists to not spread word about the wonders of the Catalan capital and not even to post the photos they take during their visits on social media. The FAVB believes that such steps could reduce the city's fame and, as a result, the tourist influx.
"We wanted to make tourists aware that they can also do something in this regard. [FABV] does not pretend to blame them, but make them aware that they are part of the problem", FAVB President Ana Menéndez Martínez de Bartolomé stated.
Residents of Barcelona have been actively combating the increasing influx of tourists, while the city secured 12th place in the most visited cities ranking last year. Opponents of "touristification" staged protests, attacking tourist buses and smashing rental cars during the summer of 2018. Others resorted to more peaceful methods, like lobbying for changes at the city Council or posting anti-tourist posters across Barcelona.
Repartim octavetes als turistes que visiten la Sagrada Família i els demanem complicitat pel #DecreixementTurístic. pic.twitter.com/K6NklGfRO5— FAVB (@FAVBcn) 30 июля 2019 г.
Locals believe that the tourists contribute to the rising food, rent and housing prices in the city, making it unbearable for its inhabitants. Some also stress that the tourists' way of life, with late-night partying, also regularly causes disturbances, spoiling the lives of ordinary Barcelonans.


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Luke 1:28 - And the angel came in unto her, and said, Hail, thou that art highly favoured, the Lord is with thee: blessed art thou among women. (Verses like Luke 1:28)
2 Thessalonians 3:16 - Now the Lord of peace himself give you peace always by all means. The Lord be with you all. (Verses like 2 Thessalonians 3:16)
Genesis 18:19 - For I know him, that he will command his children and his household after him, and they shall keep the way of the LORD, to do justice and judgment; that the LORD may bring upon Abraham that which he hath spoken of him. (Verses like Genesis 18:19)
Joshua 24:15 - And if it seem evil unto you to serve the LORD, choose you this day whom ye will serve; whether the gods which your fathers served that were on the other side of the flood, or the gods of the Amorites, in whose land ye dwell: but as for me and my house, we will serve the LORD. (Verses like Joshua 24:15)
Judges 6:12 - And the angel of the LORD appeared unto him, and said unto him, The LORD is with thee, thou mighty man of valour. (Verses like Judges 6:12)
Ruth 4:11 - And all the people that were in the gate, and the elders, said, We are witnesses. The LORD make the woman that is come into thine house like Rachel and like Leah, which two did build the house of Israel: and do thou worthily in Ephratah, and be famous in Bethlehem: (Verses like Ruth 4:11)
Psalms 118:26 - Blessed be he that cometh in the name of the LORD: we have blessed you out of the house of the LORD. (Verses like Psalms 118:26)
Psalms 127:1-3 - (A Song of degrees for Solomon.) Except the LORD build the house, they labour in vain that build it: except the LORD keep the city, the watchman waketh but in vain.It is vain for you to rise up early, to sit up late, to eat the bread of sorrows: for so he giveth his beloved sleep.Lo, children are an heritage of the LORD: and the fruit of the womb is his reward. (Verses like Psalms 127:1)
1 Timothy 6:2 - And they that have believing masters, let them not despise them, because they are brethren; but rather do them service, because they are faithful and beloved, partakers of the benefit. These things teach and exhort. (Verses like 1 Timothy 6:2)
2 Timothy 4:22 - The Lord Jesus Christ be with thy spirit. Grace be with you. Amen. (The second epistle unto Timotheus, ordained the first bishop of the church of the Ephesians, was written from Rome, when Paul was brought before Nero the second time.) (Verses like 2 Timothy 4:22)
2 John 1:10-11 - If there come any unto you, and bring not this doctrine, receive him not into your house, neither bid him God speed:For he that biddeth him God speed is partaker of his evil deeds. (Verses like 2 John 1:10)


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The Atlantic Ocean is the second largest of the world's oceanic divisions. It is bounded by the continents of America, Europe and Africa and at its polewards margins by the Arctic and the Southern Oceans. Different climatic patterns can be observed along its large latitudinal domain, which extends from the equator to sub-polar regions. Thus, different tropical and extra-tropical meteorological systems may have some influence on the characterization of precipitation regimes observed surrounding the basin, such as the Intertropical Convergence Zone (ITCZ), tropical monsoon systems, westerly storm tracks and atmospheric rivers. The subtropical regions of the Azores and South Atlantic high pressure systems are large evaporative areas which act as important moisture sources for the adjacent continents. Variations in the oceanic characteristics may influence the moisture transport towards the neighboring landmasses and alter the precipitation. The influence of climatic variability modes manifest not only over the Atlantic Ocean, but also over other oceanic regions, may also interact with the regional hydrological budget, thereby generating long periods of drought or excessive precipitation over the Atlantic rim landmasses. This Research Topic intends to highlight the advances of the scientific community in investigating the continental precipitation surrounding the Atlantic Ocean and its variability on various temporal and spatial scales.
Atlantic Ocean basin --- climate variability --- precipitation --- Droughts
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Geomorphometry is the science of quantitative terrain characterization and analysis, and has traditionally focused on the investigation of terrestrial and planetary landscapes. However, applications of marine geomorphometry have now moved beyond the simple adoption of techniques developed for terrestrial studies, driven by the rise in the acquisition of high-resolution seafloor data and by the availability of user-friendly spatial analytical tools. Considering that the seafloor represents 71% of the surface of our planet, this is an important step towards understanding the Earth in its entirety.This volume is the first one dedicated to marine applications of geomorphometry. It showcases studies addressing the five steps of geomorphometry: sampling a surface (e.g., the seafloor), generating a Digital Terrain Model (DTM) from samples, preprocessing the DTM for subsequent analyses (e.g., correcting for errors and artifacts), deriving terrain attributes and/or extracting terrain features from the DTM, and using and explaining those terrain attributes and features in a given context. Throughout these studies, authors address a range of challenges and issues associated with applying geomorphometric techniques to the complex marine environment, including issues related to spatial scale, data quality, and linking seafloor topography with physical, geological, biological, and ecological processes. As marine geomorphometry becomes increasingly recognized as a sub-discipline of geomorphometry, this volume brings together a collection of research articles that reflect the types of studies that are helping to chart the course for the future of marine geomorphometry.
bedforms --- forage fish --- Pacific sand lance --- sediment habitats --- bathymetry --- currents --- seabed mapping --- marine geology --- submarine topography --- marine geomorphology --- terrain analysis --- multibeam echosounder --- bathymetry --- DEM --- satellite imagery --- multi beam echosounder --- filter --- geomorphology --- coral reefs --- Acoustic applications --- object segmentation --- seafloor --- underwater acoustics --- Cretaceous --- Cenomanian–Turonian --- paleobathymetry --- paleoclimate --- paleoceanography --- reconstruction --- simulation --- shelf-slope-rise --- geomorphometry --- GIS --- spatial scale --- spatial analysis --- terrain analysis --- seafloor geomorphometry --- domes --- volcanoes --- digital elevation models (DEMs) --- Canary Basin --- Atlantic Ocean --- cold-water coral --- carbonate mound --- habitat mapping --- spatial prediction --- image segmentation --- geographic object-based image analysis --- random forest --- accuracy --- confidence --- global bathymetry --- Seabed 2030 --- Nippon Foundation/GEBCO --- seafloor mapping technologies --- seafloor mapping standards and protocols --- benthic habitats --- shelf morphology --- eastern Brazilian shelf --- geomorphometry --- terrain analysis --- bathymetry --- surface roughness --- benthic habitat mapping --- python --- geomorphology --- submerged glacial bedforms --- deglaciation --- sedimentation --- multibeam --- acoustic-seismic profiling --- swath geometry --- multibeam spatial resolution --- integration artefacts --- Multibeam bathymetry --- benthic habitat mapping --- multiscale --- Random Forests --- pockmarks --- automated-mapping --- ArcGIS --- Glaciated Margin --- North Sea --- Malin Basin --- Barents Sea --- bathymetry --- thalwegs --- canyons --- Alaska --- Bering Sea --- multibeam sonar --- carbonate banks --- semi-automated mapping --- polychaete --- Northwestern Australia --- Oceanic Shoals Australian Marine Park --- Bonaparte Basin --- Timor Sea --- bathymetry --- digital terrain analysis --- geomorphometry --- geomorphology --- habitat mapping --- marine remote sensing
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Radioautographic demonstration of receptors for epidermal growth factor in various cells of the oral cavity.код для вставкиСкачать
THE ANATOMICAL RECORD 222:191-200 (1988) Radioautographic Demonstration of Receptors for Epidermal Growth Factor in Various Cells of the Oral Cavity MOON-IL CHO, YU LIN LEE, AND PHILIAS R. GARANT Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook, New York 11794-8700 ABSTRACT Mouse iodinated epidermal growth factor (EGF)was localizedby light and electron microscopic radioautography in basal cells of oral epithelium, papillary cells of the enamel organ, periodontal ligament fibroblasts, preodontoblast precursor cells, and preosteoblasts of the alveolar bone of 13-day-old Sprague-Dawleyrats. The specificity of binding in these cells was suggested by an observed reduction of about 90% in the labeling when excess unlabeled EGF was injected along with the 125I-EGF. In contrast, fully differentiated cells, such as ameloblasts. odontoblasts, and osteoblasts, were only poorly labeled. Quantitative analysis of the light microscopic radioautographs revealed that the papillary cells had the highest level of labeling (5.5grains per 100 pm2 of cell area). The significance of the rather high labeling of the preosteoblasts of the alveolar bone and the fibroblasts of the periodontal ligament is unknown. However, the well-known effect of EGF in producing precocious eruption of teeth may be a consequence of an effect on these two cell types. Epidermal growth factor (EGF) was originally isolated from male mouse submandibular glands (Cohen, 1962). It is a single-chain polypeptide of 53 amino acids with a molecular weight of 6,045 Da (Carpenter and Cohen, 1979). EGF is a potent mitogen for various cell types, both in vitro and in vivo (Carpenter and Cohen, 1979; Das, 1982). It is well documented that EGF binds to specific receptors on the cell membrane of its target cells and is internalized into an endosomal compartment (Carpenter and Cohen, 1976; Haigler, et al., 1979). One of the earliest findings with respect to the in vivo effects of EGF was the precocious eruption of incisors when it was administered to newborn mice (Cohen, 1962). EGF administered in vivo binds to cells in the enamel organ of developing teeth (Martineau-Doize,et al., 1987). The in vitro binding of EGF to mouse and human odontogenic tissue a t various stages of development has also been studied (Thesleff, 1987; Thesleff, et al., 1987; Partaneu and Thesleff, 1987). These recent demonstrations of the binding of EGF to various odontogenic tissues suggests that this growth factor may have a significant role in the differentiation of the enamel organ and dental follicle mesenchyme. The recent observation of the binding of EGF in the apical tissues of a developing human premolar root (Thesleff, et al., 1987) is in good agreement with the observed association of precocious tooth eruption and EGF (Cohen, 1962; Frindik, et al., 1985). However, the mechanism responsible for precocious eruption remains unknown. In this study, we have identified the cell types that bind EGF in vivo during the development of teeth. With lZ5I-EGFradioautography, a high number of binding sites for EGF were detected on the cell membranes of basal cells of oral epithelium, papillary cells of the enamel organ, periodontal ligament fibroblasts, and @ 1988 ALAN R. LISS, INC preosteoblasts. On the basis of these observations, we speculate that accelerated eruption of teeth resulting from treatment with EGF may be due t o a direct and/ or indirect effect on periodontal ligament fibroblasts and preosteoblasts. MATERIALS AND METHODS lodination of Epidermal Growth Factor Mouse EGF (receptor grade) was purchased from Collaborative Research, Inc. (Waltham, MA) and radioiodinated by the chloramine T technique (Hunter and Greenwood, 1962; Carpenter and Cohen, 1976)to a specific activity of approximately 100 pci/pg immediately before use. Injection of l2'I-EGF A total of three 13-day-old Sprague-Dawley rats weighing 23.5 +- 0.1 g were used. lZ5I-EGFwas injected through a jugular vein under ether anesthesia. In order to study the in vivo localization of specific binding sites for EGF by radioautography, two rats were injected with 100 pci of lZ5I-EGF(S.A. = approximately 100 pci/ p.g) in 0.1 ml of 0.05 M potassium phosphate buffer, pH 7.5, containing 0.075 M NaCl. To assess the specificity of binding, a control rat was injected with 0.1 ml of 0.05 M potassium phosphate buffer, pH 7.5, containing 100 pci of KI-EGF and an excess (50 pg) of unlabeled EGF, and 0.075 M NaC1. Tissue Preparation for Radioautography At 5 min after administration of the iodinated growth factor, the animals were anesthetized by intraperitoReceived November 18, 1987; accepted March 2,1988. 192 M.-I. CHO, Y.L. LEE, AND P.R. GARANT TABLE 1. Quantitative analysis of light microscopic radioautographic silver grains over various cell types of the developing first maxillary molars of 13-day-oldrats Experimental group Control group # Grains Mean # # Cells # Grains Mean # counted counted per cell counted counted per cell # Cells Cell types Oral epithelium basal cells Enamel organ: Presecretory ameloblasts Stratum intermedium Postsecretory ameloblasts Papillary cells pulp: Odontoblasts Bone: Osteoblasts Preosteoblasts Periodontal ligament fibroblasts 117 1,734 14.8 103 401 3.9 90 63 88 95 48 31 196 4,505 0.5 0.5 2.2 47.4 91 409 4.5 111 135 1.2 89 80 116 63 864 1,496 0.7 10.8 12.9 73 134 131 228 1.8 1.7 neal injection of Nembutal (1 mg/l00 g body weight) prior to an intracardiac perfusion with lactated Ringer's solution for 20 sec. This procedure was immediately followed by perfusion with 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4, for 10 min. The maxillae were dissected free of the surrounding tissues and further fxed in Karnovsky's fixative (Karnovsky, 1965) for an additional 3 hr. After rinsing in 0.1 M cacodylate buffer for 10 min, the maxillae were decalcified for: 4 days at 4°C in 0.1 M EDTA containing 3% glutaraldehyde. The areas containing the first maxillary molars were then mesiodistally sectioned into 1mm-thick slices. These slices, along with tissue containing the developing third molars, was postfixed for 1%hours in 1%OsO, in s-collidine buffer, pH 7.4. Tissues were then dehydrated in a graduated series of cold ethanols and propylene oxide prior to infiltration with Poly Bed mixture a t room temperature for 4 hr and were placed in flat embedding molds containing fresh embedding mixture. Polymerization was accomplished at 60°C for 48 hr. Electron Microscopic Radioautography To prepare electron microscopic radioautographs, the loop method of Caro and Van Tubergen (1962) was employed as described previously (Cho and Garant, (1981b). Thin sections approximately 100 nm thick were cut from four blocks of each animal and were coated with a crystalline monolayer of Ilford L4 emulsion. After exposure at 4"C, the sections were developed with Microdol-X. QuantitativeAnalysis of Radioautographs Identification of cell types with specific binding sites for epidermal growth factor A total of 12 blocks (two blocks from each maxillar; i.e., eight blocks from the experimental rats and four blocks from the control rat) were selected and prepared for light microscopic radioautographs under conditions identical to those described previously. Photographs were taken at x 1,000 in a Zeiss photomicroscope (Carl Zeiss, Inc., NY)and printed at a final magnification of x 5,000. The number of cells and silver grains over these cells were counted on the photographic prints. The cell types Tissue Processing for Morphology subjected to quantitative analysis of radioautographs Three 13-day-oldrats weighing 23.0 f 0.1 g were an- are listed in Table 1. esthetized by intraperitoneal injection of Nembutal (1 Binding sites for epidermal growth factor per 100 pm2 of mgI100 g body weight) before intracardiac perfusion cytoplasmic surface area with 2.5% glutaraldehyde in 0.1 M sodium cacodylate In an attempt to study the relative number of binding buffer, pH 7.4, for 10 min. All subsequent procedures were identical to those described in tissue preparation sites for EGF present on different cell types, only those for radioautography. However, for morphological study, cell types with high mean values of silver grains were en bloc staining in 1%uranyl acetate in 0.1 M maleate subjected to quantitative analysis. The areas were buffer, pH 6.2, was added between postfixation in 1% measured on the photographic prints with a Kontron OsO, and dehydration in alcohol (Cho and Garant, MOP analyzer after cell boundaries were drawn with 1981a). One-micrometer sections cut in a mesiodistal a red color marker. The mean value of silver grains per 100 pm2 of cell was obtained for each cell type (Table 2). plane were stained with 1%toluidine blue. tight Microscopic Radioautography Three t o four 1-km thick sections from each block were mounted on glass slides, coated with Kodak NTB2 liquid emulsion, exposed at 4"C, and developed as previously described (Cho and Garant, 1981b). The radioautographs were subsequently stained with 1%toluidine blue in sodium acetate buffer. RESULTS Light Microscopic Morphology In 13-day-oldrats used in this study, the mesial cusp of the first maxillary molar was located directly under the oral epithelium. Root development was well advanced. The collagenous fibers of the periodontal ligament were arranged obliquely from the root surface EPIDERMAL GROWTH FACTOR RECEPTORS IN THE ORAL CAVITY 193 TABLE 2. Quantitative analysis of light microscopic radioautomaphic silver grains over unit area (100 pn2) of cell volume Cell types # Grains/100 fim2 Basal cells of oral epithelium Papillary cells in association with postsecretory ameloblasts Preosteoblasts Periodontal ligament fibroblasts 3.4 5.5 1.9 2.1 Light microscopic radioautographs from the experimental animals contained numerous silver grains, mostly restricted to the basal cells (Fig. 2a). Within the basal cells, the majority of silver grains appeared to be located at both the basal and lateral portions of the cells (Fig. 2c). The apical portion of the cell was relatively free of silver grains (Fig. 2c). Electron microscopic radioautographs confirmed these observations (Fig. 2d). The number of silver grains on the cells of the basal layer was reduced dramatically from 14.8/cell in the experimental tissues t o 3.9kell in the control tissues (Fig. 2b, Table 1). Enamel organ Light microscopic radioautography of the enamel organ in the experimental animals clearly showed that neither the presecretory, secretory, and postsecretory ameloblasts nor the cells of the stratum intermedium were labeled (Fig. 3a, b). Heavy labeling was observed along the peripheral region of all papillary cells (Fig. 3b). Ultrastructural morphology of this region showed a large number of microvillilike structures projecting into the intercellular spaces. Electron microscopic radioautography revealed specific localization of silver grains along the cell membrane proper, but not on the microvilli (Fig. 3d, el. The number of silver grains was greatly reduced over papillary cells of the control rat, although the pattern of labeling remained the same as in the experimental group (Fig. 3c, Table 1). Bone Fig. 1. Light micrograph showing the mesial developing root of the first maxillary molar of a 13-day-old rat x 100. Low-magnification micrograph (inset)illustrates the mesial half of the developing tooth with its enamel organ under the oral epithelium (ep). x 23. The rectangular area of inset shows an area similar to that shown in Fig. 1. A = ameloblasts; AB = alveolar bone; D = dentin; E = enamel; ES = enamel space; HR = Hertwig's root sheath Od = odontoblasts; P = pulp; PD = predentin; PDL = periodontal ligament. In areas of active bone formation, light microscopic radioautography from both the experimental and control animals demonstrated that clearly identifiable osteoblasts (including osteoclasts; result not shown) remained unlabeled (Fig. 4a, b). However, prominent labeling was observed over fibroblastic cells near the osteoblasts (Fig. 4a). A small number of grains remained in association with these cells in the control group (Fig. 4b, Table 1). Electron microscopic radioautography clearly confirmed that the labeled fibroblastic cells were relatively immature cells, perhaps preosteoblasts in the process of migrating toward the prebone surface between adjacent osteoblasts (Fig. 4c). coronally toward the alveolar bone. Odontoblasts were Periodontal ligament The developing periodontal ligament of the first maxlocated along the predentin surface in the pulp (Fig. 1). illary molars of 13-day-old rats was characterized by Radioautographic Localization of Binding Sites for '251-EGF fewer collagen fibrils and more fibroblasts, compared Oral epithelium with a fully developed and functional periodontal ligThe oral epithelium covering the first developing ament (Fig. 5a). Periodontal ligament fibroblasts exmaxillary molars was a typical stratified squamous epi- hibiting a high degree of polarization and orientation thelium (Figs. 2a,c). were heavily labeled along their cell boundaries in the 194 M.-I. CHO, Y.L. LEE, AND P.R. GARANT Fig. 2. Ramoautographs showing localization of '25I-EGF on oral epithelium. a: Light microscopic radioautograph of oral epithelium from an experimental animal reveals a heavy labeling with silver grains on the basal cells (arrowheads).x 120. b The number of grains is dramatically decreased on the basal cells from the control animal x 120. c: Cells of the cornified (C),granulosum (G),and spinosum (S)layers are free of labeling, in contrast to heavy labeling of basal layer (B).X 900. d Eledon microscopic radioautography demonstrates localization of silver grains (arrowheads) along the basal and lateral protions of the cell membrane of the basal cells (B) from an experimental animal. x 2,700. S = spinosum layer; F = fibroblasts. Fig. 3. Radioautographs showing the localization of '"I-EGF on the enamel organ. a: Light microscopic radioautograph of developing third molar shows that both presecretory amelohlasts (A) and cells of the stratum intermedium (SI) of an experimental animal remain unlabeled. x 350.D = dentin. b Papillary cells (P)of an experimental animal are, however, heavily labeled, whereas only a small number of grains are present on the papillary cells from the control animal ( c ) x 400. A = amelohlasts; B = blood vessel. d Electron microscopic radioautograph of papillary cells (P) from an experimental animal localizes the silver grains on the cell membrane. x 1,200.A = ameloblasts; M = microvilli. e: The silver grains (arrowheads) are present on the cell membrane of papillary cells, hut not on the microvilli (MI.X 2,700. 196 M.-I. CHO, Y.L. LEE, AND P.R. GARANT Fig. 4. Radiographs showing localization of lZ5I-EGFon bone tissue. a: Preosteoblasts (arrowheads)from an experimental animal demonstrate heavy labeling. x400. The number of grains on those from the control animal (b) are reduced dramatically. x 400. Osteoblasts (Ob) and osteocytes (OC) remain unlabeled. B = bone; pb = prebone. c: Electron micrsocopic radioautography of an experimental animal exhibiting a preosteoblast (POB) migrating (arrow) toward prebone (PB) between two osteoblasts (OBI. x 2,000. Fig. 5. Radioautographs showing the localization of 1251-EGFin the periodontal ligament. a: Light microscopic radioautographs from an experimental animal demonstrating numerous silver grains over periodontal ligament, x 120. AB = alveolar bone; D = dentin. b: Only a small number of grains are present on the periodontal ligament from the control animal. X 120. AB = alveolar bone; D = dentin. c: Most silver grains are located over cell membrane of periodontal ligament fibroblasts (F). x 500. d Electron microscopic radioautograph illustrating the localization of silver grains (arrowheads)near the cell membrane of the fibroblasts (F). X 2,000. 198 M.-I. CHO, Y.L. LEE, AND P.R. GARANT experimental animals (Fig. 5a, c). A marked reduction in number of silver grains was observed over these cells from the control group (Fig. 5b, Table 1).Electron microscopic radioautography clearly revealed the localization of silver grains along the cell membrane of periodontal ligament fibroblasts (Fig. 5d). Odontoblasts Odontoblasts at different stages of cytodifferentiation were identified along the forming root surface (Fig. 1). The precursor cells, aligned next to the root sheath, did not show any special orientation or evidence of alignment to the epithelial cells (Fig. 6a). As differentiation progresses, odontoblasts become closely packed, elongated, and polarized and show evidence of secretion of the dentinal matrix (Fig. 6b). Mature odontoblasts, characterized by the presence of odontoblastic processes, form predentin and dentin (Fig. 6c). Light microscopic radioautography of 1251-EGFdemonstrated that neither newly differentiated odontoblasts nor fully mature odontoblasts bind EGF (Fig. 6b, c). However, a small number of grains were found over the cells in the subodontoblastic layer, as well as over odontoblast precursor cells close t o the root sheath (Fig. 6a, c). Quantitative Analysis of Radioautographs Silver grain counts in experimental tissue indicated that a small number of grains (less than 2.2 grains per cell) were observed over ameloblasts, stratum intermedium cells, osteoblasts, osteocytes, osteoclasts (result not shown), and odontoblasts (Table 1).However, a high number of grains per cell was observed over papillary cells (47.4) in association with the postsecretory ameloblast, basal cell (14.8) of oral epithelium, periodontal ligament fibroblast (12.9), and preosteoblast (10.8) (Table 1). In contrast, the number of silver grains localized over these cells types was signficantly reduced in control tissue to approximately one-tenth the number recorded in the experimental groups (Table 1). This difference may indicate that the silver grains observed in the experimental animals represent specific binding sites for EGF. The relative number of specific binding sites for EGF present on each cell type was obtained by counting the silver grains per unit area (100 pm2)of cell volume for each cell type. It was found that papillary cells showed the highest number (5.5),followed by basal cells of oral epithelium (3.4), periodontal ligament fibroblasts (2.1), and preosteoblasts (1.9) (Table 2). DISCUSSION It has been established that 1251-labeledhormones and EGF maintain biologic activity and an ability to bind to their specific receptors on the cell membrane Fig. 6. Radioautographs depicting localization of '"1-EGF durign den(Carpenter and cohen, 1976; Bergeron et al., 1977, 1978; tinogenesis. a: The epithelial root sheath (E)and the subadjacent odonBarazzone et al., 1980; Warshawsky et al., 1980; Fehl- toblast precursor cells (arrow) slightly labeled. x 400. P = pulp cells. mann et al., 1982; Silver et al., 1982; Nanney et al., b Newly differentiated odontoblasts (Od) are unlabeled, while some 1986). Radioautography has been used for the in vivo silver grains are loeated on subodontoblasticfibroblasts, shown at higher in 6e (arrow). x 400. c: Mature odontoblasts (Od) show and in vitro demonstration of receptor sites for calci- magnification no labeling. A small number of silver grains are associated with fibroblast tonin (Warshawsky et al., 1980), insulin (Bergeron et (arrow) adjacent to the odontoblast layer. ~ 5 0 0D. = dentin; OP = al., 1977), growth hormone (Barazzone et al., 1980),and odontoblastic process; PD = predentin. EPIDERMAL GROWTH FACTOR RECEPTORS IN THE ORAL CAVITY 199 EGF (Nanney et al., 1984, 1986; Martineau-Doize et EGF on root formation and vascular changes during al., 1987; Thesleff, 1987; Thesleff et al., 1987; Partinen tooth eruption. In addition to root formation and vasand Thesleff, 1287).Binding sites for lZsI-EGFin normal cular changes, the development of the periodontal lighuman skin were located primarily on the mitotically ament and the formation and resorption of bone have active basal keratinocytes and appeared in diminished been regarded as major factors responsible for tooth numbers as the degree of differentiation of these cells eruption (Ten Cate, 1980a, b; Avery, 1987).Among these progressed (Nanney et al., 1984,1986). Our results sup- factors, the periodontal ligament has been regarded as port these findings; in addition, our electron micro- the most likely source for generating the force required scopic radioautographic results demonstrate that EGF for tooth eruption. Although the generation of traction receptors are located primarily along the basal and lat- within the periodontal ligament remains unclear (Ten eral cell membranes of the basal cells and only rarely Cate, 1980a, b), it has been suggested that collagen molecules and/or the fibroblasts themselves may be the on the apical surface of these cells. Recently, McKee et al. (1986) investigated the entry major sources of contractile force generation (Thomas, and penetration of various I251-labeledproteins with dif- 1964; Bellows et al., 1982a, b; Ten Cate, 1980a, b). Considering that collagen fibrils are a product of fiferent molecular weights into the enamel organ and enamel of the rat incisor. They reported heavy labeling broblasts and that their organization and turnover are of papillary cells by lZ5I-EGFbut were unable to deter- manipulated by fibroblasts, it may be concluded that mine whether this labeling was specific or nonspecific. EGF may influence the rate of tooth eruption by its By comparing the labeling in animals injected with lZ5I- effect on periodontal ligament fibroblasts. FurtherEGF only and animals injected with both W-EGF and more, a mitogenic effect on preosteoblasts might inan excess of unlabeled epithelial growth factor, Mar- crease the rate of alveolar bone formation, which could tineau-Doize et al. (1987) obtained evidence suggesting also contribute to tooth eruption. a ligand-receptor interaction. Our results agree with their findings. ACKNOWLEDGMENTS Of interest is the fact that of all cells examined in This research was supported by grants #DE03745 our study, the papillary cells had the highest number and #DE06165 from the National Institute of Dental of binding sites for epithelial growth factor (5.5 per 100 Research, National Institutes of Health. We thank Mrs. pm2 unit area of cell volume), even exceeding that of Champa Codipilly for her excellent technical assisthe basal cells of the oral epithelium (3.4 per 200 pm2)>.tance. The help of Mrs. Kris Vandenberg in preparation The physiologic signficance of this abundance of EGF of the manuscript is greatly appreciated. binding by papillary cells remains to be established. Note added in proof: The possible roles of EGF on Although the mitogenic effect of EGF, its binding and tooth eruption was further studied by Rodes et al. (Deintracellular degradation have been intensively studied vel. Biol. 121:247-252,1987) and Topham et al. (Devel. in cultured fibroblasts (Carpenter and Cohen, 1974, Biol. 124532443, 1987). 1979), the in vivo localization of specific binding sites LITERATURE CITED for EGF on fibroblasts has received little attention. Nanney et al. (1984) were unsuccessful in localizing Barrazzone, P., M.A. Lesniak, P. Gorden, E. Van Obherghen, J.-L. Carpentier, and L. Orci 1980 Binding, internalization, and lysosomal binding sites for EGF on the fibroblasts of human skin, association of '"I-human growth hormone in cultured human l p using both radioautographic and immunocytochemical phocytes: A quantitative morphological and biochemical study. J . Cell techniques. They postulated that skin fibroblasts may Biol. 87:360-369. express only a small number of EGF receptors. We also Bellows, C.G., A.H. Melcher, U. Bhargava, and J.E. Aubin 1982a Fibroblast contrating three-dimensional collagen gels exhibit ultrastruchave observed only a small number of W-EGF binding ture consistent with either contraction or proteii synthesis. J. sites on fibroblasts located in the connective tissue adUltrastmct. Res 78:178-192. jacent to oral epithelium (manuscript in preparation). Bellows, C.G., A.H. Melcher, and J.E. Aubin 1982a Association between In view of the small amount of EGF that is bound by tension and orientation of periodontal ligament fibroblasts and exogenous collagen fibres in collagen gel in vitro. J . Cell Sci 58: skin and oral epithelial fibroblasts, the relatively high 125-138. amount observed to be associated with fibroblasts of Bergeron, J.J.M., G. Levine, R. Sikstrom, D. OShaughnessy, B. Kopriwa, the periodontal ligament (2.1 per 100 pm2) and with N.H. Nadler, and B.I. Posner 1977 Polypeptide hormone binding sites preosteoblasts (1.9 per 100 pmz)(Table 2) takes on spein uiuo: Initial localization of Y-labeled insulin to hepatocyte plasmalemma as visualized by electron mi-pe radioautography. Proc. cial significance. Our results indicate that fibroblasts Natl. Acad. Sci. U.S.A. 74.5051-5055. residing on different areas of body connective tissue Bergeron, J.J.M., B.I. Posner, Z. Josefsberg, and R. Sikstrom 1978 Inmay deploy different concentrations of receptors for EGF tracellular polypeptide hormone receptors. The demonstration of speand may be under different degrees of epithelial growth cific binding sites for insulin and human growth hormone in Golgi fractions isolated from the liver of female rats. J. Biol. Chem. factor control (manuscript in preparation). 253:4058-4066. One of the earliest findings of an in vivo effect for Cam, L.G., and R.P. Tubergen 1962 High resultion autoradioautography. EGF was precocious incisor eruption when this factor I. Methods. J . Cell Biol. 15t173-188. was injected into newborn mice (Cohen, 1962). Neither Camenter. G.. and S. Cohen 1976 '"I-labeled human eDidermd erowth ;ador: 'Binding, internalization, and degradation h human" fibrothe mechanisms for tooth eruption in general nor the blasts. J. Cell Biol. 71:159-171. mechanism responsible for precocious incisor eruption Carpenter, G., and S. Cohen 1979 Epidermal growth factor. Ann. Rev. resulting from injection of EGF are fully understood. Biochem. 48:193-216. Of interest are the recent studies of Thesleff showing Cho, M.I., and P.R. Garant 1981a Sequential events in the formation of collagen secretion granules with special reference to the development signficant uptake of EGF by apical tissues of the deof segment-long-spacing-hke aggregates. Anat. Rec. 199: veloping root (Thesleff et al., 1987).They reported heavy 309-320. labeling of blood vessel walls and dental follicle mes- Cho, M.I., and P.R. Garant 1981b An electron microscopic radioautographic study of collagen secretion in periodontal ligament fibroblasts enchyme. They speculated on the possible influence of 200 M.-I. CHO, Y.L. LEE, AND P.R. GARANT of the mouse: I. Normal fibroblasts. h a t . Rec. 201.577-586. Cohen, S. 1962 Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the newborn animal. J . Biol. Chem. 237r1555-1562. Das, M. 1982 Epidermal growth factor: Mechanisms of action. Int. Rev. Cyt~l.,78r233-256. Fehlmann, M., J.-L. Carpentier, L.C. Alphonse, P. Thamm, D. Saunders, D. Brandenburg, L. Orci, and P. Freychet 1982 Biochemical and morphological evidence that the insulin receptor is internalized with insulin in hepatocytes. J . Cell Biol. 93:82-87. Frindik, J.P., S.F. Kemp, R.H. Fiser, H. Schedewil, and M.J. Elders 1985 Phenotypic expression in Donohue syndrome (leprechaunism): A role for epidermal growth factor. J. Pediatr. 107t428-430. Haigler, H.T., J.A. McKanna, and S. Cohen 1979. Direct visualization of the binding and internalization of a fenitin conjugate of epidermal growth factor in human carcinoma cells A 431. J. Cell Biol. 81t382395. Hunter, W.M., and F.G. Greenwood 1962 Preparation of lSII-labelled human growth hormone of high specific activity. Nature (Lond.) 194:495-496. Karnovsky, M.J. 1965 A formaldehyde-glutaraldehyde futative of high osmolality for use in electron mircroscopy. J. Cell Biol. 27:137A. Martineau-Doize, B., W.H. Lai, H. Warshawsky, and J.J.M. Bergeron 1987 Specific binding sites for epidermal growth factor in bone and incisor enamel organ of the rat. In Development of Diseases of Cartilage and Bone Matrix. Alan R. Liss, Inc., New York, pp, 389-399. McKee, M.D., B. Martineau-Doize, and H. Warshawsky 1986 Penetration of various molecular weight proteins into the enamel organ and enamel of the rat incisor. Arch. oral Biol. 31: 287-296. Nanney, L.B.,M. Magid, C.M. Stoscheck, and L.E. King 1984 Epidermal growth factor binding and receptor distribution in normal human skin and appendages. J . Invest. Dermatol. 83:385-393. Nanney, L.B., C.M. Stoscheck, M. Magid, and L.E. King 1986 Altered lZ5I-epidermalgrowth factor binding and receptor distribution in psoriasis. J. Invest. Dermatol. 84t260-265. Portanen, AM., and I. Thesleff 1987 Localization and quantitation of 1251-epidermalgrowth factor binding in mouse embryonic tooth and other embryonic tissues at different developmental stages. Dev. Biol. 120:186-197. Silve, C.M., G.H. Hradek,A.L. Jones, andC.D.Arnaud 1982Parathyroid hormone receptor in intact embryonic chicken bone: Characterization and cellular localization. J. Cell Biol. 94:379-386. Ten Cate, A.R. 1980a Physiological tooth movement: Eruption and shedding. In: Oral Histology, Development, Structure and Function. The C.V. Mosby Company, St. Louis, pp. 270-283. Ten Cate, A.R. 1980b Tooth eruption. In: Organ's Oral Histology and Embryology. Bhaskar, S.N., ed. The C.V. Mosby Company, St. Louis, pp. 371-385. Thesleff, I. 1987 Epithelial cell rests of Malazziz bind epidermal growth factor intensely. J . Periodont. Res. 22:419-421. Thesleff, I., A.M. Portanen, and L. Rihtniemi 1987 Localization of epidermal growth factor receptors in mouse incisors and human premolars during emption. Eur. J. Orthop. 9:24-32. Thomas, N.R. 1964 The role of collagen maturation in alveolar bone growth and tooth eruption. J . Dent. Res. 43:947. Warshawsky, H., D. Goltzman, M.F. Rouleau, and J.J.B. Bergeron 1980 Direct in vivo demonstration by radioautography of specific binding sites for calcitonin in skeletal and renal tissues of the rat. J. Cell Biol. 85:682-694.


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Our DJs for hire are some of the most vibrant, exciting and electrifying musicians in the country and abroad. Our talented DJs will not only bring the party but will also keep it going all night long.
Our DJs have travelled all over the UK and internationally, performing and perfecting their craft. These DJs for Hire have played for audiences of all sizes and ages, ranging from nightclubs to large arenas.
This means that our DJs can create the perfect party atmosphere for you and your guests. Our DJs have wide-ranging repertoires of thousands of songs that will get everyone moving on the dance floor. They are also able to create and tailor specific playlists for your event, especially if you have a particular theme for it such as '80s, disco or Motown.
Our DJs for hire have earned their stripes and have an amazing wealth of experience under their belts. They are the perfect form of entertainment for your event whether it is an exciting wedding, raving music festival, or unique corporate event you are planning our DJs are best entertainment option.
DJs have rapidly increased in popularity over the past years with the emergence of exciting professional DJs like; Calvin Harris, David Guetta, Skrillex and Diplo.
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With Chinese New Year soon approaching on the 25th January 2020, we have created a comprehensive guide on planning the best Chinese New Year Party!
Our DJs will create the same vibe that they do and will transport you to a massive Vegas party with just their music.
Our talented musicians only use the best equipment to deliver a sound that is high-quality and entertaining. Whatever the occasion, venue or theme of your event, our versatile DJs will achieve the desired look and vibe for it.
They can whisk you away by creating an Ibiza party island vibe, or even transport you to a beachside party. Our DJs can even create a stylish and thrilling music festival atmosphere for your event.
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Can we help with DJs?
Speak to one of our event managers to see how we can help with all your entertainment for events.Get in Touch