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4 bedroom detached house for saleGhyllside Road, Northiam
Offers in Excess of
Do you like this property?
Call: 01797 720024
Property Description
Key features
- Incredibly spacious 4 bedroom detached chalet style residence
- Highly sought after Village location
- Walking distance to local amenities
- Large open plan L-shaped living / dining room
- Modern kitchen
- Three generous first floor bedrooms
- Private rear garden with summerhouse
- Off road parking to front
Full description
CHAIN FREE - An incredibly spacious four bedroom detached chalet style residence offering well balanced and versatile living accommodation located within the highly regarded and popular Village of Northiam. Accommodation comprises a 20' l-shaped living / dining room with wood burning stove and french doors to a private garden, a contemporary kitchen, ground floor 4th bedroom or optional dining room, office / study (formerly a garage), three generous first floor bedrooms and main family bathroom. Outside offers a private rear garden with summerhouse, patio area and decked porch leading to a outside utility room. To the front offers ample parking over hard standing.
Front - Area of hard standing to side aspect providing off road parking for two vehicles, close board gate to rear garden, dwarf brick wall with iron gate to front garden, paved path to entrance porch, external lighting, mature hedgerow and flowering shrubs with brick edged shingled bed to front, external floor mounted oil boiler.
Entrance Porch - External UPVC obscure glazed front door with matching side light,further obscure glazed window to front, tile effect vinyl flooring, ceiling light, internal door to cloakroom, internal hardwood door to hall.
Cloakroom - Internal door from porch, window to side aspect, tile effect vinyl flooring, push flush WC, pedestal wash basin, single radiator, ceramic wall tiling and wall mounted mirror.
Hallway - Internal hardwood door from porch, carpeted flooring, carpeted staircase to first floor landing, double radiator, ceiling light
Bedroom 4 / Dining Room - 3.81m x 3.81m (12'6 x 12'6) - Internal door, wood effect plank vinyl flooring, window to side aspect with radiator, wall mounted Honeywell thermostat, corner pedestal wash basin, various power points, under stair storage cupboard.
Kitchen - 4.98m x 2.62m (16'4 x 8'7) - Internal door from hall, tile effect vinyl flooring, window and external door to front elevations, further window to side, radiator below, kitchen hosts a selection of contemporary high gloss matching base ans wall units beneath granite effect laminated work surfaces with matching splash backs, inset single stainless bowl with mixer tap, inset four ring induction hob, extractor canopy and light above, fitted eye level BEKO oven and grill, space for freestanding fridge / freezer, under counter space for washing machine and dishwasher, various above counter level power points, ceiling light.
Living / Dining Room - 6.30m x 6.12m (20'8 x 20'1) - Internal door from hall, open plan L-shaped room, window to side aspect with radiator below, french doors with matching sidelight to rear, further windows to rear to dining end, freestanding wood burning stove upon granite hearth, various power points and TV connections, ceiling fans, internal door to office (formerly the garage).
Office / Study - 4.60m x 2.44m (15'1 x 8') - Internal door from main living room, carpeted flooring, window to rear aspect with radiator below, ceiling and wall lights, wall mounted consumer unit.
Stairs And Landing - Carpeted staircase and landing, window to rear aspect with radiator below ,eaves storage cupboard, access panel to loft over, ceiling light, double power point.
Bedroom 1 - 3.78m x 3.58m (12'5 x 11'9) - Internal door, carpeted flooring, window to side aspect with radiator below, corner shower enclosure, vanity unit, eaves storage cupboard, ceiling light.
Bedroom 2 - 4.45m x 3.66m (14'7 x 12') - Internal door, carpeted flooring, window to side aspect with radiator below, ceiling light, fitted wardrobes with mirrored sliding doors complete with hanging rails and shelving, various power points.
Bedroom 3 - 4.42m x 3.89m l-shaped room (14'6 x 12'9 l-shaped - Internal door, carpeted flooring, timber wall and ceiling panelling, window to rear with radiator below, various power points, ceiling light.
Bathroom - 3.38m x 3.25m l-shaped room (11'1 x 10'8 l-shaped - Internal door, carpeted flooring, timber wall and ceiling panelling, double radiator, two tilt and turn obscure glazed windows to front aspect, corner shower enclosure, corner bath suite, push flush WC, vanity unit with wall mounted mirror, ceiling light.
Rear Garden - Privately enclosed rear garden with area of hard standing from rear elevations leading to a level rear lawn, close board gate to side aspect, patio area with stocked raised bed, detached summer house ( 9'5 x 9'5) with power and lighting, covered decked area leading to a utility room with power and light.
Agents Note - None of the services or appliances mentioned in these sale particulars have been tested. It should also be noted that measurements quoted are given for guidance only and are approximate and should not be relied upon for any other purpose.
Services - Oil fired central heating system - gas connection available in roadside.
Mains drainage
Local Authority - Rother District Council - Band E
More information from this agent
To view this media, please visit the on-line version of this page at www.rightmove.co.uk/property-for-sale/property-85301126.html
Energy Performance Certificate (EPC) graphs
Listing History
Nearest station
- Winchelsea (6.2 mi)
Nearest schoolsUse the school checker
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Nearest station
- Winchelsea (6.2 mi)
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Market Info
Disclaimer - Property reference 29143302. The information displayed about this property comprises a property advertisement. Rightmove.co.uk makes no warranty as to the accuracy or completeness of the advertisement or any linked or associated information, and Rightmove has no control over the content. This property advertisement does not constitute property particulars. The information is provided and maintained by Rush Witt & Wilson, Northiam. Please contact the selling agent or developer directly to obtain any information which may be available under the terms of The Energy Performance of Buildings (Certificates and Inspections) (England and Wales) Regulations 2007 or the Home Report if in relation to a residential property in Scotland.
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When most people hear the word "hospice," they typically think about the worst — the loss of a loved one.
Conversations surrounding end of life are ones that people usually don't like to have, as it makes them uncomfortable, said Vanessa Hensley — the patient care and volunteer coordinator for Medi Home Health and Hospice in Boone. Death can be hard and stressful for families and patients, and that's when hospice services come in to help alleviate that strain.
Both Hensley and Lisa Caviness — the public relations and marketing specialist at Caldwell Hospice and Palliative Care — said that studies show that patients live longer with the help of hospice. Both agencies help with the physical, emotional and spiritual aspects of patients who are ill.
"It's not about dying. Our care is about living," Caviness said. "We want you to live the fullest life possible; the best quality no matter what quantity remains."
November is National Hospice and Palliative Care Month, which focuses on the specialized form of health care for people with serious and life-limiting illnesses, according to the National Hospice and Palliative Care Organization.
"At the center of hospice and palliative care is the belief that each of us has the right to live and die pain-free and with dignity, and that our families will receive the necessary support to allow us to do so," stated NHPCO.
Medi Home has been serving Watauga, Ashe and Avery counties for more than 30 years, although it has operated in the past under different names. Medi Home serves under the umbrella of Medical Services of America. The agency operates with the help of Medical Director Mark Harter — who has served in the community for 40 years — as well as social workers, nurses, volunteers and a chaplain.
Medi Home's palliative care services are offered under its Home Health program. Danielle Shook, Medi Home's account executive, said the agency believes that if someone has a life-limiting illness, whether acute or chronic, the patient should have access to as many resources as possible. Through palliative care services, patients can receive nursing, speech/physical/occupational therapies, social work and aide services weekly.
To be considered for hospice care, a patient must be diagnosed with a terminal illness. These illnesses can be anything from Alzheimer's/dementia, heart failure, COPD/lung disease, ALS/Parkinson's, cancer/leukemia and kidney/liver failure, according to Shook.
Hensley said skilled nurses will routinely check on patients while monitoring their vitals and what medications they are taking. Nursing assistants can help with baths, light house work or patient grooming. Medi Home now offers music therapy when staff can play music with patients and help them write songs. Hensley said music can be used to help with patient agitation for those who may have dementia or other illnesses.
Social workers with Medi Home can help patients with items like filing advanced directives or funeral arrangement forms. Volunteers can offer respite services to allow caregivers the ability to run errands or other tasks.
Hensley said in her role she sits with patients and just talks to them about topics such as what career path they had, where they grew up and things they've accomplished.
"They give more to me than I could ever give to them," Hensley said.
Dawn Ward is a nurse at Watauga Medical Center and has used hospice services twice in the last few years from Medi Home. Ward lost her brother, Johnny Poss, in May 2018 after he was exposed to Agent Orange and developed cancer. Ward said her brother declined fast, and was only with hospice for a month and a half.
Ward's mother, Pat Poss, died in February of this year after battling COPD, and received hospice services for two days.
While each of her family members were with hospice for a short period of time, she said she couldn't express how amazing the hospice workers were with her loved ones. She said Medi Home made her loved ones feel comfortable and cared for. Ward said she has often referred people to Medi Home for hospice services.
"It was so reassuring having the same people and same care help me through a really hard time," Ward said. "I wouldn't have wanted anybody else to be in there. We couldn't have done it without them."
Medi Home does not offer a hospice housing unit, but rather meets people in their home or senior care facility. The agency can come evaluate anyone for hospice services, and inquiries can be made to Medi Home at (828) 265-3388.
Opening in 1982, Caldwell Hospice is a nonprofit that started offering services in Watauga, Ashe and Avery counties in 2014. It was the first hospice agency to offer a free standing in-patient care unit. The agency averages about 250 people a day, according to Caviness.
It has two patient care units with one in Lenoir and one in Hudson, and it helps patients who may have a pain crisis who can't get it under control at home or who need respite care. With respite care, patients can stay for up to five days every 30 days. Those who need residential care, who may not have a caregiver at home, can stay in the unit in a residential capacity, Caviness said.
Caldwell Hospice also offers a palliative medicine program as a clinical collaboration with Appalachian Regional Healthcare System for those living with serious illnesses and chronic issues. Caviness said this program helps prevent frequent hospitalizations, navigate complex care situations and adds an extra layer of support for the patient.
Hospice patients are typically those with a medical diagnosis of six months or less of life, and each patient is given their own personal care team that develops a plan of care that meets that patient's specific needs. Hospice services can help patients maintain independence and make personal decisions about their illness or disease, organize personal affairs, participate in spiritual services and spend time with loved ones.
Through these services, Caldwell Hospice can offer resources for cardiac care, pulmonary care, dementia care in addition to various physical and emotional therapies. It also provides services for veterans. Additionally, Caldwell Hospice offers a resource called Legacy Project life stories. The agency will transcribe, video record or audio record stories or messages patients may want to leave for family members.
Caviness recalled a time several years ago when a young mother was in hospice care with the goal of living through her son's first birthday. She wanted her son to remember her after she died, so Caldwell Hospice video recorded her reading her favorite stories for him.
"There were a lot of tears," Caviness said. "It was rough for us all."
Both Medi Home and Caldwell Hospice provide support for families after the death of a loved one — both can offer bereavement services for those in the community whether they were a patient with them or not. Caldwell Hospice offers grief support for up to 13 months after the death of a patient.
Caviness said some people see entering hospice as giving up hope, but hospice workers see the situation as redefining hope. She said it allows patients to take control of the things they can and determine how they want to live their last days.
"We want to disassociate the stigma that is related to hospice," Shook said. "It is one of the only ways that an individual can drive their own health care and lay out their own plan of care. Hospice is there to support the patient and families' decisions, not make them for them."
For more information on Medi Home, visit www.msa-corp.com/companies/medihomehealthhospice.aspx. More information on Caldwell Hospice can be found at www.caldwellhospice.org.


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ron1973 - in reply to curryfamily2016
depending on your PCB there is a 4 pin slot open labeled "rotary" if not you can disconnect the Y-axis and plug in your driver there. Zeroing can be done manually just remember you have to hit the "stop" switch manually before it can go to your offset. The cutting software has a "rotary" setting just click on it.


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1. Please give an overview of your role and what this involves on a day-to-day basis:
My role involves looking at new claims that the insurers have brought into Aon and then setting them up on the system. I watch claims progress and upload reports and complete settlements if they are needed. I always make sure my emails are up to date and categorise them so that the most important things are done first. I have a lot of responsibility as I deal with the international casualty with my colleague [This section of the comment has been removed by a member of the RateMyApprenticeship Team because it did not meet our site terms and conditions]. I deal alot with offices across the world and have built up relationships with colleagues at Aon as well as colleagues across different insurers who I deal with on a daily basis meaning the flow things that need to be done is easy.
2. Have you learnt any new skills or developed existing skills?
I have learnt lots of new skills when it comes to setting up claims onto the system as 6 months ago I would have never been able to do that. I am much more confident in myself when it comes to doing tasks and have become more responsible for getting work done myself without someone checking on it.
3. To what extent do you enjoy your programme?
I enjoyed the program a lot. I definitely think I made the right decision about not going to university as I can get my qualifications and I do not have any debt. The programme has enabled me to become more independent and allows me to step out of my comfort zone as I have never had a full time job before this one. I like the learning side and think that I am now a much more independent learner as the tasks need to be completed without the help of anyone in order to build up my portfolio.
4. How well organised/structured is your programme?
I would say that the programme is quite structured as we have specific deadlines to meet for tasks and they have a path they want us to follow when completing the exams. We have meetings with our mentor once a month and are always allowed to call or email if we need help with anything. We also have a session before each exam where a coach comes in and they run through some of the content which makes it easier to understand as we have to teach ourselves the content so hearing it from another perspective makes it seem simplier.
5. How much support do you receive from your employer?
I receive a lot of supporter from my employer with regards to my exams and the portfolio we need to complete. I will ask for time with him to be able to go through things that need to be completed and if I need any extra help I know he will be able to provide it. He will be coming to my meetings with my mentor which will enable him to see a different perspective to the program from someone who is teaching us what to do.
6. How much support do you receive from your training provider when working towards your qualifications?
We receive a lot of support from our training provider. He is always available on call and through email. If we ever have any queries he will always respond to email. After failing my exam he was helpful in the process to rebook and make sure that I was okay and helped me go through some of the content in the next session we had.
7. How well do you feel that your qualification (through your training provider) helps you to perform better in your role?
The qualification enables me to have a much wider range of knowledge regarding the insruance market rather than just the area that I work in. This enables me to not only understand the area which I work in but if I was to change the role I do within the insurance industry I may have a better understanding of another role. It is also nice to have a wider knowledge of the industry that I work in and I may be able to apply different knowledge to my role.
8. Are there extra-curricular activities to get involved in at your work? (For example, any social activities, sports teams, or even professional networking events.)
There are lots of activities to get involved with, including sports teams. There are networking events during christmas periods. As an apprentice you don't see as much advertisement for these events as we have to balance the revision and the workload but after the course I could possibly do more activites,
9a. Would you recommend Aon to a friend?
9b. Why?
I would recommend Aon to a friend as I have not had any problems with the process of getting the job and all the people that I have met through the job have been very nice. Everyone is very welcoming and there are always people there to support what you want to do. The flexible time allows you to do your job and work it round out of work things which means that you always have time to do your work and do stuff outside.
10. What tips or advice would you give to others applying to Aon?
I would say believe in yourself. I had gone through the whole interview process believing I would never get the job as the people on my assessment day were so much better than me. There is also the process of your exams. Make sure you revise and get the grades needed to get into Aon as although they may offer you the job, you will not get in if you do not have the grades.
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The Takeaway: The premier course in the Southern Hemisphere and one of the finest tracks on Mother Earth. Pictures don't begin to sufficiently show the curves, subtleties, and movement that this property enjoys. Architecturally it is a masterpiece that is only trumped by the Composite course as having the ultimate property for golf in Melbourne. Grade A+
Quick Facts
Designer: Alister MacKenzie in 1931
Cost: Private Private (Interstate, International, and Reciprocal visitors welcome) Click for membership information
Phone Number: 61 3 9599 0500
Course Website: Official Website - Visit Royal Melbourne Golf Club (West)'s official website by clicking on the link provided.
Directions: Get here! - Cheltenham Road, Black Rock, Victoria 3193
Photos: See additional photos of Royal Melbourne Golf Club (West)
Accolades: Ranked 3rd in Australia by Golf Course Gurus.
What to Expect: Simply one of the finest golf courses in the world. This is the course that sets the standard for Australia golfing greatness and an attraction that draws in players from all over the world. Heading into my round I was a bit worried that Royal Melbourne would be overrated because the pictures I saw depicted little movement in the property and it was difficult to identify the architectural greatness. After the first hole, my worries increased. After the second hole, I was feeling a little better. By the time I came to the world class 5th hole I knew I would be experiencing golfing nirvana. The movement throughout the property exceeds virtually anything else in the Melbourne area with holes 4 - 11 being a particularly excellent stretch. It becomes more impressive to consider how good the holes are and the undulations in the property when you see the primitive shovel and plow equipment that was used along with a horse to do construction. The West course is not long, but instead relies on top shelf green complexes and testy pin locations to put some teeth in the layout while tempting you with risk/reward opportunities that can bite you hard if you get too greedy. The one-shotters stand out at Royal Melbourne with brilliant bunkering and green surfaces that are demanding in both slope and speed while some of the par fours are as good as anything you'll ever play. Crossing the road and getting to the flatter part of the property on holes 13 - 16 is less inspiring and a worthy omission when Royal Melbourne routs the Composite course that is used for major competitions such as the President's Cup. The vast majority of players will walk the course and caddies are available, particularly for visitors, but there are some medical exceptions that would allow a motorized cart. In the end, Royal Melbourne is world class. The staff and membership is extremely friendly, accommodating, and not near as stuffy as one would expect as a similar caliber course in the United States. A true fan of golf course architecture hasn't completed their studies without a visit to Royal Melbourne; it is the fundamental of minimalist greatness.
By the Numbers
|Mens (Blue)
|Mens (White)
|Ladies (Red)
Individual Hole Analysis
Signature Hole: 5th Hole – 176 Yard Par 3 –
Best Par 3: 7th Hole – 148 Yards –
Best Par 4: 6th Hole – 428 Yards –
Best Par 5: 4th Hole – 504 Yards –
Birdie Time: 10th Hole – 312 Yard Par 4 –
Bogey Beware: 11th Hole – 455 Yard Par 4 –


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Comments 10
Joe Bird
fake, nellie, plastic.. pure affectation. Wish people could just be themselves instead of these fake creations.
I am amazed. He is a lovely, gorgeous looking boy, it is sad that he feels he is ugly and needs to be a girl.
I'm not surprised every comment is implying this boy feels like he isn't being himself or that he feels ugly.
Like fuck, don't you maybe, just maybe feel like this form of expression is for him, in fact, him being himself?
I hate old, narrow-minded faggots. You like em fem and twinky, just not too fem or too girly huh?
brian d
To me his best look was when he was wearing glasses and a hat


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5.3 oz., pre-shrunk 100% cotton
Dark Heather is 50/50 cotton/polyester
Sport Grey is 90/10 cotton/polyester
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Target has some but they are so far that's the cheapest I've seen, but I haven't checked too many places yet, still working on it. We will be getting in on this Jason Voorhees and Michael Myers Trick or Treat Halloween shirt next year! Now I just need to find a pumpkin that can be reused of a good size! Keep an eye out after Halloween this year maybe they'll be super discounted and still available. I wonder how one of those trick or treat pumpkins for kids would hold paint? Just turn it around so the face faces the wall.
Yes, that was my plan last year but didn't work out I'll check a couple more places soon otherwise I'll just get the one at the target. Hey guys first timer here. Is it normal for Hollywood to release the Jason Voorhees and Michael Myers Trick or Treat Halloween shirt ticketing info and such later than Orlando? Potter is a logistical problem. Forbidden Journey is such a big ride and needs a certain amount of time to rest between closing and opening, otherwise, it won't open for the park the next day on time. I've also heard that WB doesn't want it open for the event, but the first one is definitely a thing.
One of the employees told me that JK Rowling doesn't want harry potter associated with a Halloween scare which is why she requested that they close that section during horror nights hours. I don't know but before we even touch Harry Potter we need to seriously do something about that Terror Tram. It's been going downhill since 2010 and I'd honestly rather not have it if it's getting worse and worse.


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Quote by Rumi
What is planted in each person's soul will sprout.
Similar Quotes
Since love grows within you, so beauty grows. For love is the beauty of the soul. Saint Augustine
Positive thinking will let you do everything better than negative thinking will. Zig Ziglar
Another reason why we must love our enemies is that hate scars the soul and distorts the personality. Martin Luther King Jr.
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What greater thing is there for two human souls than to feel that they are joined to strengthen each other and to be at one with each other in silent unspeakable memories.
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text: On 01/12/2013 05:21 PM, Dan Kegel wrote: >> The library in question is using static vc runtime linkage, so _getptd() >> is linked in > > That's somewhat worrisome. So you have multiple instances > of the C runtime library active in the same app? > > Maybe you're not unloading that dll gracefully enough. > > Might be heap corruption. I wonder if Valgrind might be helpful. > > I second the suggestion to post a minimal testcase. Here's a link to a zip file that includes the prebuilt .exe and .dll windows binaries that crash in wine. Plus the code. https://dl.dropbox.com/u/97386125/msvcrt-dll-problem.zip So, again, the bug is that MSVCRT calls are failing when called from a DLL that has been reloaded. If the calls come from a thread and if the DLL is built with static MSVCRT runtimes. There is no crash for the program in Windows. I also tried rebuilding the code for winelib, and it doesn't appear to have the same behavior. So the problem doesn't happen if Wine's MSVCRT is used, only if a windows DLL that uses static MSVCRT linkage is reloaded. Thanks -- mo


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text: fashion, New York Anna Wintour Street Style Michael Kors New York Fashion Week SS19 3rd October 2018 No Comments Share this:Click to share on Facebook (Opens in new window)Click to share on Pinterest (Opens in new window)Click to share on Twitter (Opens in new window)Click to share on Tumblr (Opens in new window) Related Anna WintourMichael KorsNew York Fashion WeekSS19Street Style Previous Post Next Post You may also like 2nd January 2017 Jan-Michael Quammie at London Fashion Week SS17 2nd February 2017 Street Style Paris Fashion Week Dior Haute Couture SS17 18th February 2017 London Fashion Week Street Style AW17 Leave a Reply Cancel reply Your email address will not be published. Required fields are marked *Comment Name * Email * Website Save my name, email, and website in this browser for the next time I comment. Notify me of follow-up comments by email. Notify me of new posts by email.


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Papa Murphy's in Lubbock, TX
Papa Murphy's locations found near Lubbock
View Map
Papa Murphy's Take 'N' Bake Pizza - 8205 Indiana Ave
Rating: 4.7
31 Reviews
8205 Indiana Ave, Lubbock TX 79423
(806) 792-2200
Papa Murphy's Take 'N' Bake Pizza - 421 Frankford Ave suite b
Rating: 4.4
33 Reviews
421 Frankford Ave suite b, Lubbock TX 79416
(806) 796-0600
Papa Murphy's Take 'N' Bake Pizza - 6319 82nd St
Rating: 3.8
19 Reviews
6319 82nd St, Lubbock TX 79424
(806) 698-0022
Lubbock, Texas
Papa Murphy's is a take-and-bake pizza company, founded in 1995, in Vancouver, Washington, United States. It is known as the fifth-largest pizza chain in the United States, and it operates in over 1500 locations. Papa Murphy's has a menu that contains pizzas, gourmet delight dishes, stuffed, salads, sides and sweets. It also allows their customers to create their own pizza, through the C.Y.O. section available in the menu.
Similar Stores in Lubbock
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Zilhão, Miguel and Ansorg, Marcus and Cardoso, Vitor and Gualtieri, Leonardo and Herdeiro, Carlos and Sperhake, Ulrich and Witek, Helvi (2011) Higher-dimensional puncture initial data. Physical Review D, 84 (8). 084039. ISSN 2470-0010. https://resolver.caltech.edu/CaltechAUTHORS:20111222-115301586
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We calculate puncture initial data, corresponding to single and binary black holes with linear momenta, which solve the constraint equations of D-dimensional vacuum gravity. The data are generated by a modification of the pseudospectral code presented in [ M. Ansorg, B. Bruegmann and W. Tichy Phys. Rev. D 70 064011 (2004)] and made available as the TwoPunctures thorn inside the Cactus computational toolkit. As examples, we exhibit convergence plots, the violation of the Hamiltonian constraint as well as the initial data for D=4,5,6,7. These initial data are the starting point to perform high-energy collisions of black holes in D dimensions.
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|© 2011 American Physical Society. Received 8 August 2011; published 18 October 2011. We thank Andrea Nerozzi and the anonymous referee for useful suggestions and discussions. M. Z. and H.W. are funded by FCT through Grants No. SFRH/BD/43558/2008 and SFRH/BD/46061/2008. U. S. acknowledges support from the Ramón y Cajal Programme of the Ministry of Education and Science of Spain, the FP7-PEOPLE-2011-CIG Grant CBHEO, No. 293412, NSF grants PHY-0601459, PHY-0652995, and the Sherman Fairchild Foundation to Caltech. This work was supported by the DyBHo–256667 ERC Starting Grant and by FCT— Portugal through projects PTDC/FIS/098025/2008, PTDC/FIS/098032/2008, PTDC/CTE-AST/098034/2008, and CERN/FP/116341/2010. This research was supported by an allocation through the TeraGrid Advanced Support Program under Grant No. PHY-090003, an allocation by the Centro de Supercomputación de Galicia (CESGA) under project ICTS-2009-40 and allocations at the Barcelona Supercomputing Center (BSC) under projects AECT-2011-2-0006 and AECT-2011-2-0015. Computations were performed on the TeraGrid clusters Kraken at the National Institute for Computational Sciences (NICS) of the University of Tennessee and Trestles at the San Diego Supercomputing Center (SDSC), the Milipeia cluster in Coimbra, Finis Terrae at the Supercomputing Center of Galicia (CESGA), the supercomputer Caesaraugusta at the Institute for Biocomputation and Physics of Complex Systems (BIFI) at the University of Zaragoza, HLRB2 of the Landesrechenzentrum (LRZ) in Munich, MareNostrum at the Barcelona Supercomputing Center in Barcelona, and on the Blafis cluster at the University of Aveiro.
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Different gut microbiome composition in obese Guizhou minipigs between female and castrated male
Gut microbiome lives in the intestinal tract of animals and plays an important role in almost all life processes. Gut microbiome balance is beneficial to health, and imbalance leads to many diseases, one of which is obesity epidemic. However, gut microbiome is also influenced by host hormone, and different gut microbiome composition is observed between the sexes. Here, we studied whether castrated male Guizhou minipigs with obesity own the same gut microbiome composition and microbial function predictions with those in obese females. We sequenced the hypervariable regions V3 to V4 of bacterial 16s rRNA of fecal samples collected from our study subjects. We observed that the operational taxonomic units were small, which suggested that the abundance of gut microbiome may be influenced by low genetic diversity of host. Our results also suggested that the castrated male has different gut microbial composition compared to the obese female. An increasing Firmicutes/Bacteroidetes ratio was observed in both castrated male and obese female groups, which suggested that the main adipogenic gut microorganism in obese Guizhou minipigs in our studies is the same with that in other obese mammals. However, we also observed that there were function prediction differences of obese Guizhou minipigs between female and castrated male, which suggested that the influence of gut microbiome on obesity between them is different.
We are grateful for everyone who contributed to our project and appreciate Shanghai Majorbio Bio-pharm Technology Co., Ltd. for their sequencing and data analysis on cloud platform. We thank Yanjun Wu and Arome Solomon Odiba for the English revision.
The study is supported by fund from central special fund for local science and technology development [Qian Ke Zhong Yin Di [2016] 4007], fund from project for growth of youth researcher of Education department of Guizhou province [Qian Jiao He KY No. [2016] 182], and fund from the Administration of Traditional Chinese Medicine of Guizhou Province.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
Supplementary material
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