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The stars must've been aligned just right when Nick van de Wall (better known as Afrojack) saw his first ray of light. Music was his first love since his early youth. He started playing piano when he was only five. Being a very creative student, Afrojack spent most of his time listening to all kinds of music while studying. It was no surprise that it didn't take him long to turn the tables and start making music instead of just listening to it. At the age of 11, Afrojack gets hold of a program called Fruity loops. At this point in time his talent and passion for creating music truly surfaces. Rapidly acquainting himself with and appreciating Fruity Loops, it becomes his program of choice, which he uses to this day. Afrojack finishes school and decides to proceed with his musical career. Not being able to make a living out of it yet, he starts to work part-time in bars and catering. Now he has more than enough time to discover the club scene in Rotterdam, starting with his first night out in the old club Las Palmas when he was only 16 years old. After this, many club nights, side-jobs, small DJ-gigs for the fun of DJing and a lot of studio time followed.Learn more
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For any DJ and wanna-be producer, "hustle" can be an over-overstated attribute and yet, for the right person, it's an under-appreciated compliment from those in the know. Hustle is the absolute definition of both the man and the meteoric career of international DJ, Roger Sanchez. From a humble beginning in NYC to multi-year residencies in Ibiza; from digging in dusty crates to headlining festival main-stages around the world, Roger is truly a self-made man and dance music star. 2015 is proving to be one of Roger Sanchez's most creative and prolific years to date, with the continued success of his S-Man moniker and the first releases under his own name for some time.Spring Summer Women Flat College Style Casual Fish Mouth Breathable Comfort shoes Ladies Platform Walking Sandal
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DJ, producer, label boss. The young artist continues to prove himself as one of Holland's finest exports in House music, demonstrating a solid production career with releases spanning a collection of top labels, including a long term relationship with Defected Records, whilst continuing to push himself as a DJ with a distinctive style that works across the big stages as well as packed out clubs.Learn more
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Claude VonStroke is an alias created by Barclay Crenshaw in 2005. He's known to be one of the most well-respected label owners and DJs in dance music. His record label, DIRTYBIRD, has signed acts like Justin Martin, Julio Bashmore, Catz n Dogz, Riva Starr, Tim Green, Breach, Eats Everything, Shadow Child and Shiba San. The label continues to innovate the underground dance scene, releasing house, funk, dirty-bass and electronica music.Learn more
It's time to get serious! Dance After Dark on Dance FM is the only place in the UAE to hear DJ sets and shows from both the planet's top international stars including the world's top three DJs, as well as the crème-de-la-crème of local artists.Learn more
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From London via global hot spots like New York, Miami, Los Angeles, Las Vegas, Malaysia and now Dubai, it's been an incredible journey for DJ, producer and Record Label owner CN Williams. Forged in the raw rave heat of the 90's, he became one of London's stars, with a long-running residency at UK super-club Escape that lasted nearly 15 years. During that time, he was crowned the best resident DJ from 2005-2008 at the Dance Music Awards and played headline sets at legendary UK clubs Ministry of Sound, Fabric, The End and Pacha. That success led to residencies with the biggest club brands in the world as Defected, Hed Kandi, Fierce Angel and Space all came calling. Further awards followed as he won the Best Ladies Night DJ in Dubai from Hype Magazine in 2016. An in-demand DJ throughout his career, he's opened and closed for the who's who of music – from Carl Cox to Roger Sanchez, Shapeshifters to Derrick Carter, Trevor Nelson to Jazzy Jeff he's been there, done that and got the sweaty t-shirt to prove it.Learn more
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It's time to get serious! Dance After Dark on Dance FM is the only place in the UAE to hear DJ sets and shows from both the planet's top international stars including the world's top three DJs, as well as the crème-de-la-crème of local artists.A-LING Women Open Toe Chunky Block Heeled Sandals Ankle Leg Strappy Booties High Heels Pumps shoes
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Some DJ careers are built on hype. John Digweed's career is built on substance. One of the planet's most popular DJs, his sets never fall out of favour with clubbers the world over, as his relentless global gig schedule testifies. From ethereal, swirling cinematic soundscapes to energy-infused bass-bothering tech growlers, a new generation of clubbers are learning what we already know - that there's nothing quite like being locked into a John Digweed set.Btrada Sneakers for Women,Casual Lace-up Running shoes, Comfortable Jogging Walking shoes
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Chris always stood and still stands for solid Techno on the deeper side of the musical spectrum. His sets are sonic journeys between darkness and light, always far away from the beaten tracks of the musical mainstream. Being a kid from the 80s and growing up with Synth Pop bands like Depeche Mode and electronic dance acts like Nitzer Ebb amongst many others, his musical background also contains the melancholy, the vocals and the melodies from this era, which he increasingly blends into his current sets. People who exclusively know him for his fast and unrelenting Techno sets of the past might be surprised about those new facets he is adding to his at all times dynamic sound.Learn more
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Nicole Moudaber is an event promoter, record label founder, radio host, and one of the most passionately followed DJ/producers in the world. The one-woman army is marching her muscular take on techno all over the globe, winning fans not only with her music, but her way of being, that's at once uncensored, inclusive, and fierce.Btrada Women's Flower Wedge Sandals Clip Toe Elastic Ankle Strap Platform Fashion Thong Flip Flops Beach shoes
DJ and producer, Adam Cotier makes his mark on the UAE nightlife scene, following a 2018 venture to Dubai to open up new underground nightclub labeled 'A Musical Institute' and lead the line as Resident DJ.Island Sea Turtles Ocean Women Casual Sneakers Flat Athletic News Comfortable
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Few come as well versed in family values as that of long serving techno legend Adam Beyer. A husband, father and certified underground icon, the Stockholm-basedDJ/producer has come to embody one of the most consistent and carefully constructed legacies to emerge from Europe and hold influence across the continents. From festival main stages and coveted club floors through to the global airwaves and Beatport charts alike, Beyer's extended musical legacy is felt across the board.Learn more
It's time to get serious! Dance After Dark on Dance FM is the only place in the UAE to hear DJ sets and shows from both the planet's top international stars including the world's top three DJs, as well as the crème-de-la-crème of local artists.Pattern Of Dandelion Women Casual shoes Sneakers Canvas Athletic News Comfortable
Filling your Friday mornings with the hottest dance music in the UAE and the world. Join Dance FM from 10 AM to 2 PM every Friday and unwind, as we play your favourite anthems, throwbacks and the newest future Obsessions out there.AllhqFashion Women's Pull On Leather Handmade Pointed Closed Toe High Heels Solid Pumps shoes
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The Genres of music Hijakz plays consists of Commercial Remix/House, Dance, Trance, House, Progressive House, Tech House, Deep House, Soulful House, Funky House, EDM & many more. Chris previously known as "DJ-CJ' has performed his musical talent all over the UK for the first 8 years of his career building up a name as a commercial Dance and House DJ, He then decided to bring his talent to the UAE to which he has honed his skills and become a huge fan favourite to the people of Abu Dhabi.MsMushroom Woman's Velvet Classic Latin Dance Wedding shoes 3 Heel
Multi-award-winning DJ and resident at Zero Gravity and Pacha, Soulninja is one of the region's most in-demand artists. Whether playing for high-end clubs such as Cavalli, Movida, and Mahiki, or on the festival front, (where recent gigs have seen him share the bill with Tiesto, Kings of Leon, and The Killers), Soulninja always delivers, with his intoxicating blend of seamless multiple genre mixing.Learn more
Kryder's musical talents don't follow a trendtailor-made sets the trend. His music transcends language and captivates the ear. Delivering a sound so genre defying it is easily considered "too" fresh to label. Never one to wake up and say "I want to make an electro record", he lets the feel and groove of the moment propel his inspiration. His invigorating take on big room firepower has found a massive audience, both on the dance floor and within the dance music industry. Just as well, his peak time, mind numbing, genre busting sets have a story to tell, and Kryder's ability to game-changing that story keeps the crowds craving more.Learn more
Sam Feldt (born August 1, 1993) is a DJ and music producer from Boxtel, Netherlands. His music career was started in 2013 with several remixes, including On Trees And Birds And Fire, Group I Am Oak, etc.Learn more
Black sunglasses, a baseball cap flipped backwards and a laptop: His visual trademarks simultaneously serve as his most important tools.Women's shoes Leather Summer Novelty Comfort Sandals Block Heel for Casual Party,Evening Red Almond
Regarded as one of the founding fathers of the Future House movement, it's evident why Dutch DJ/Producer Don Diablo is seen as a pioneer of the dance music world. Currently #11 in the DJ Mag Top 100 Poll - making him the highest-placing artist in the Future House category - Don has earned praise from talent and tastemakers across the globe for major hits such as 'On My Mind', 'Universe', 'AnyTime', 'Chemicals' and 'Cutting Shapes' and most recently released his sophomore album 'FUTURE' in the beginning of 2018.AnMengXinLing Fashion Ankle Boots Women Novel High Heel Pointed Toe Leather Western Studded Boots Side Zipper Plus Size
With 2009's multi-platinum One Love, two-time-Grammy-winning artist/producer/DJ David Guetta cemented his status as a genre-busting hitmaker whose in-your-face fusion of electro and hip-hop beautifully warps the pop landscape.AI BAO Women's Fashion Chain Bag Classic Contrast Shoulder Messenger Bag
As two wise Chemically enhanced men once said, 'The brothers gonna work it out' and it's an adage the Smokingroove brothers have taken to heart as they've become one of the most in-demand production and DJ duos around. Starting off the main part of their career in their native home of London, they've cut their teeth on the underground radio & club scene from the mid 90s and are still going stronger than ever. Production-wise, they specialise in deep, dark, Chicago-flavored House music with a Jacking vibe; the sort that's seen them sign records to Roger Sanchez's Stealth Records, DJ Sneak's Magnetic Recordings and a string of releases on their own Illuminati Recordings imprint. The sort that has got them charted by every DJ worth his salt (and tequila), the sort of infectious, funk-laden, booty bouncing music that forces the body to inadvertently shake itself into a dance. And when they're not busy creating big-boomed bass beauties, they're usually found in smoke-filled clubs keeping the underground house scene very much alive and kicking.Learn more
Beyond their breathtaking melodies and explosive performances, you'll find dance music's closest friendship. Dutch powerhouse duo Lucas & Steve have seen a momentous rise to stardom in recent years, climbing charts and making their DJ Mag Top 100 debut at #64 in 2017.Gioseppo Women's 46047-p Hi-Top Slippers Black
Fadil El Ghoul (born in 's-Hertogenbosch, 2 April 1986), better known by his stage name R3hab (pronounced "rehab"), is a Dutch DJ and record producer of Moroccan origin. Alongside Afrojack and Chuckie, R3hab is one of the proponents of the modern Dutch house subgenre, although his style and sound at the time were usually darker, somewhat reminiscent of early psytrance or gabber compositions. During the 2012 WMC in Miami, United States, R3hab won the IDMA Best Breakthrough Artist Award.Women's Fine Heel Sandals-Stylish Round-Head Sandals-Pure color Velvet After Bag Luxury Water Drill Sandals-Comfort Heel shoes Zhhzz
From being a young, music wunderkind to an influential artist in electronic dance music, Nicky Romero has quickly become a force to be reckoned with. The DJ and producer is also a label mogul who is at the forefront of EDM.Learn more
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It's time to get serious! Dance After Dark on Dance FM is the only place in the UAE to hear DJ sets and shows from both the planet's top international stars including the world's top three DJs, as well as the crème-de-la-crème of local artists.Learn more
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New York City native and well respected US music industry vocal coach/producer, Stacy Smith has landed in the UAE. Making her radio presenting debut here on Dance FM, Stacy will shake up your Saturday with a well-crafted selection of commercial and deep house hits.Learn more
Greg Stainer & Olly Wood a.k.a. HOLLAPHONIC present Saturday Social from 2pm to 4pm. The show features the hottest releases played by Greg and Olly, and crazy banter guaranteed to make it the best way to spend your Saturday afternoon!Learn more
Having always loved music, Mark knew that he was destined to be a DJ and began developing a great collection of music and mixing skills since the young age of 15. His database spans multiple genres but Mark's true passion lies in playing a variety of House Music.Learn more
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Nora En Pure is the Swiss Deep House & Indie Dance music sensation that is rippling through the dance music world with her beautiful blend of electronic beats. Leading listeners out of the darker realms of deep house with intricate instrumentals and enlightening melodies, every track she gets her hands on turns to gold, and every performance she gives leaves the audience in awe.Learn more
One of the most dedicated, hard-working characters in the industry, Swiss-Italian DJ, producer and label head, EDX, has spent the past twenty-five years at the forefront of electronic music. Founder of the internationally renowned record company Sirup Music in 2002, which has released hits from the likes of Avicii, Armin van Buuren, Steve Angello, Tiësto and more — and in joint-venture with Enormous Tunes his 'Helvetic Nerds' crew of Nora En Pure, Daniel Portman, Croatia Squad and Sons of Maria — Maurizio's own vast catalogue of chart-topping original productions and remixes has amassed hundreds of millions of streams and an adoring international fan-base in the process.Learn more
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Hedkandi is an international music and lifestyle brand that encompasses the international clubbing scene. Since its start as a humble funky house compilation brand, Hedkandi has grown to become the global sensation that has more than 100 albums that feature their feel-good signature sound.Grasshoppers JANEY SEASONALS Women's Oxford 12 B(M) US Black-Black
There are few individuals in the world of dance music who can claim the kind of influence Pete Tong has had over the last twenty years.Learn more


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James MasonJames Neville Mason (; 15 May 1909 – 27 July 1984) was an English actor. Mason achieved considerable success in British cinema before becoming one of Hollywood's biggest stars. He was the top box office attraction in the UK in 1944 and 1945, with notable films including ''The Seventh Veil'' (1945) and ''The Wicked Lady'' (1945). He starred in ''Odd Man Out'' (1947), the first recipient of the BAFTA Award for Best British Film.
He starred in a number of successful British and American films from the 1950s to the early 1980s, including ''The Desert Fox'', ''A Star Is Born'', ''20,000 Leagues Under the Sea'', ''Lolita'', ''North by Northwest'', ''The Prisoner of Zenda'', ''Journey to the Centre of the Earth'', ''A Touch of Larceny'', ''Bigger Than Life'', ''Julius Caesar'', ''Georgy Girl'', ''The Deadly Affair'', ''Age of Consent'', ''Heaven Can Wait'', ''The Boys from Brazil'', ''The Verdict'', ''Mandingo'', ''Murder by Decree'' and ''Salem's Lot.''
Mason was nominated for three Academy Awards, three Golden Globes (winning the Golden Globe in 1955 for ''A Star is Born'') and two BAFTA Awards throughout his career. Following his death in 1984, his ashes were interred near the tomb of his close friend, fellow English actor Sir Charlie Chaplin. Provided by Wikipedia
by Mason, James
Published 1805
Published 1805
Printed for J. Harding, and J. Archer, Dublin
by Mason, James
Published 1808
Published 1808
Printed and sold by J. and W. Eddowes
A review of the principal arguments in favour of restricting the importation, and allowing the exportation, of corn
by Mason, James
Published 1814
Published 1814
Printed for J. Ridgway
by Mason, James Eric, Traoré, Issa, Woungang, Isaac
Published 2016
Published 2016
Springer International Publishing

subset: commoncrawl


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Runt-related transcription factor 1 (RUNX1) is essential for normal hematopoiesis. RUNX1 mutations have rarely been reported in chronic myelomonocytic leukemia (CMML). We examined RUNX1 mutations in 81 patients with CMML at initial diagnosis. Mutational analysis was performed on bone marrow samples by direct sequencing of all reverse transcription PCR products amplified with three primer pairs that cover the entire coding sequences of RUNX1b. Thirty-two RUNX1 mutations were detected in 30 patients (37%); 23 mutants were located in the N-terminal part and 9 in the C-terminal region. The mutations consisted of 9 missense, 1 silent, 7 nonsense and 15 frameshift mutations. Two patients had biallelic heterozygous mutations. There was no difference in overall survival between patients with and without RUNX1 mutations, but a trend of higher risk of acute myeloid leukemia (AML) progression was observed in mutation-positive patients (16/30 vs 17/51, P=0.102), especially in patients with C-terminal mutations (P=0.023). The median time to AML progression was 6.8 months in patients with C-terminal mutations compared with 28.3 months in those without mutations (P=0.022). This study showed for the first time a high frequency of RUNX1 mutations in CMML. C-terminal mutations might be associated with a more frequent and rapid AML transformation.
Chronic myelomonocytic leukemia (CMML) is classified as one of the subtypes of myelodysplastic syndrome (MDS) in the French–American–British system,1 whereas according to the World Health Organization classification, CMML is categorized to be an MDS/myeloproliferative disease rather than MDS per se.2 Over the last decade, genetic alterations in patients with MDS/CMML have been a subject of investigations. RAS mutations are frequent events in CMML.3, 4 Fusion genes involving the PDGFβ receptor gene at 5q33 are characterized by a distinct phenotype of CMML-eos.5 Efforts are continuously being made to identify additional genetic aberrations involved in the development of CMML. We have investigated FLT3 and NRAS mutations in patients with CMML,6, 7 and we also examined the incidence of mutations in the CEBPα myeloid transcription factor in patients with CMML who later had acute myeloid leukemia (AML) transformation.8 However, all these mutations accounted for a minor proportion of patients with CMML. Other genetic aberrations require identification.
Runt-related transcription factor 1 (RUNX1) is essential for normal hematopoiesis and differentiation.9, 10 RUNX1 is also known as AML1 and acts to regulate the expression of various genes specific for hematopoiesis, including G-CSF, IL-3, T-cell receptor and myeloperoxidase.11 Earlier studies have shown that chromosomal translocations or mutations in the coding region of RUNX1, especially in the Runt homology domain (RHD) (exons 3–5), disrupt the function of RUNX1.11, 12 RUNX1 mutations have been described in the M0 subtype of AML,13, 14 radiation-associated and chemotherapy-related MDS or AML,15, 16 or in patients with de novo high-risk MDS;17, 18, 19 however, only a small number of patients with CMML have been examined for RUNX1 mutations,20 and the frequency and patterns of RUNX1 mutations in CMML remain to be defined. In this study, we analyzed a large cohort of patients with CMML to define the prevalence and types of RUNX1 mutations. In addition, the mutation status of the RUNX1 gene was also correlated with the clinicohematological features and outcome in CMML patients to determine its clinical and prognostic relevance. To the best of our knowledge, this series is the largest one of CMML that has been examined for RUNX1 mutations and we showed a high frequency of RUNX1 mutations occurring preferentially at RHD with nonsense or frameshift patterns. We also found that C-terminal mutations of RUNX1 in CMML predicted an increased risk and shorter time to AML transformation.
Materials and methods
Patients and experimental samples
Beginning in 1991, 81 patients with CMML were diagnosed and treated in the Division of Hematology-Oncology at Chang Gung Memorial Hospital, Taipei, Taiwan. Bone marrow (BM) samples were obtained with informed consent from all patients at initial presentation. The study was approved by the Institutional Review Board, Chang Gung Memorial Hospital. All patients were followed up until death or for up to 88 months. All the 81 BM samples were re-evaluated and reclassified according to the World Health Organization system.2 Thirty-three patients had progressed to AML, which was defined as the presence of ⩾20% blasts in BM or peripheral blood. None had an earlier history of radiation exposure or chemotherapy.
There were 55 CMML1 and 26 CMML2 patients. Cytogenetic analysis was performed successfully in 53 patients. Cytogenetic findings were divided into three categories, namely, good (normal, -Y, del[5q], del[20q]), poor (complex or chromosome 7 anomalies) and intermediate (all other abnormalities), according to the criteria developed by the International MDS Risk Analysis Workshop.21 Risk scores were calculated according to the International Prognostic Scoring System.21
All patients with CMML were managed with supportive care, 45 of them received low-dose cytarabine or an oral chemotherapeutic agent (hydroxyurea, melphalan or 6-thioguanine) before transformation to AML.
Cell fractionation
The mononuclear cells from BM samples were obtained by Ficoll-Hypaque density gradient centrifugation (1.077 g/ml; Amersham Pharmacia, Buckinghamshire, UK) and cryopreserved in 10% dimethylsulfoxide and 20% fetal bovine serum at −70 °C or in liquid nitrogen until test.
DNA, RNA extraction and cDNA preparation
Genomic DNA and RNA were extracted from frozen BM mononuclear cells as described earlier.22 RNA was reversely transcribed to cDNA with the superscript II RNase H2 reverse transcriptase kit (Invitrogen Corporation, Carlsbad, CA, USA).
cDNA PCR or DNA PCR assay with direct sequencing for RUNX1 mutation analysis
The cDNA PCR was carried out in an Expand Long Template PCR system (Roche, Mannheim, Germany), which is composed of buffer 3 with a unique enzyme mix containing a mixture of Taq DNA polymerase and Tgo DNA polymerase (1.25 U in 25 μl); the latter is a thermostable DNA polymerase with proofreading activity.23 The Roche product was added with 0.5 mM dNTP, 0.75 μl (25 mM) MgCl2, 1.6 M Betaine and three primer pairs (1 μM/each primer), with the sequences of primers and their nucleotide position shown in Supplementary Table 1, which cover the coding sequences from exons 3 to 8 of RUNX1b cDNA (GenBank accession number D43968). The PCR was performed on a DNA thermal cycler using a program consisting of 35 cycles at 95 °C for 30 s, 58 °C for 30 s and 68 °C for 1 min, with an initial preheating at 94 °C for 3 min and a final step for 5 min at 68 °C. PCR products were electrophoresed on a 2% agarose gel, gel-purified with a MinElute Gel Extraction kit (Qiagen, Hilden, Germany) and sequenced in both directions with BigDye Terminators v3.1 Cycle Sequencing kits on an automated ABI PRISM 3730 DNA Analyzer (Applied Biosystems Inc., Foster City, CA, USA), according to the manufacturer's instructions. Sequencing results containing mutations were repeated using DNA samples. Genomic DNA PCR amplification was performed using another exon-specific primer pair with earlier published sequences for mutation confirmation.24
Cloning analysis
For samples harboring multiple mutations, cloning analysis was carried out to clarify whether the mutations were on the same allele or on different alleles. The PCR product was run on a 2% agarose gel, then cut from the gel, purified and subcloned into the pCRII-TOPO vector (Invitrogen). At least 18 clones were subsequently sequenced.
Statistical analysis
Fisher's exact test, the χ2 analysis, the unpaired t-test and Wilcoxon's rank-sum test were used whenever appropriate to make comparisons between groups. The Kaplan–Meier analysis was used to evaluate survival. Differences in survival were assessed using the log-rank test. Statistical analyses were carried out using an SPSS software version 8.0 for Windows (SPSS Inc., Chicago, IL, USA). In all analyses, P-values were two-tailed and values <0.05 were considered statistically significant.
Frequencies and patterns of RUNX1 mutations in patients with CMML
RUNX1 mutations were detected in 30 of 81 CMML patients (37%) at initial diagnosis (Supplementary Figure 1). Twenty-one patients had 23 mutations (72%) located in the N-terminal part and the remaining 9 patients had single mutations located in the C-terminal region. The patterns of the 32 mutations consisted of 9 missense mutations, 7 nonsense mutations, 15 frameshift mutations and 1 silent mutation. As shown in Figure 1 and Table 1, seven patients had eight missense mutations at RHD and one patient had a missense mutation at the C-terminal region, all resulting in single amino-acid substitutions. An additional patient had a silent mutation (Ile87Ile). Four nonsense mutations were located in the RHD that generate termination codons resulting in truncated RUNX1 proteins. An additional three patients had identical nonsense mutations at the transactivating domain (Arg293). Eight patients had nine frameshift mutations at the N-terminal region resulting in truncation of the proteins. Five patients had frameshift mutations, one at RHD and four in the C-terminal part, all generating termination codons within the 3′-untranslated region.
Two patients had two mutations and one (no. 4) had two missense mutations in RHD; cloning analysis showed that 14 clones harbored 238 C>T (Arg80Cys) and another 8 clones harbored 251 C>A (Thr84Asn). Another patient (no. 21) had two frameshift mutations in RHD; eight clones harbored 297_298insGGAC resulting in Thr101fsX111 and nine clones harbored 343_344insGG resulting in Ala115fsX118, the remaining one clone was wild type. One patient (no. 26) had 780_797 duplication and 798 T>C; cloning analysis revealed that the mutations were located on the same allele, which resulted in frameshift and a stop at codon 460. Together, except for the two patients carrying biallelic heterozygous mutations, the remaining were monoallelic heterozygous mutations.
Activating mutations of FLT3 or RAS gene in CMML patients with RUNX1 mutations
Cooperating mutations of FLT3 and RAS genes were also analyzed in CMML patients harboring RUNX1 mutations (Table 1); one patient had FLT3-TKD (Asp835Tyr), three patients had NRAS mutations and one had a KRAS mutation. Taken together, 16.7% (5/30) of CMML patients carrying RUNX1 mutations had cooperating mutations of FLT3 or RAS genes. The correlation between cooperating mutations and AML transformation is also shown in Table 1. Only one patient harboring a C-terminal mutation (no. 30) had a cooperating mutation with NRAS (Gly12Asp).
Clinicohematological characteristics and outcome of patients with CMML and RUNX1 mutations
Of the 53 CMML patients who had cytogenetic examinations, 30 had normal karyotypes. Of the patients with RUNX1 mutations, 11 had normal karyotypes with one partial tandem duplication of MLL; trisomy 13, trisomy 21, monosomy 7 and complex anomaly were detected in one patient each, and none had -5/5q- (Table 1). A comparison of clinicohematological features between CMML patients with and without RUNX1 mutations was carried out (Supplementary Table 2). No differences were observed with respect to sex, blood counts, percentage of blasts in blood, subtype of CMML, cytogenetic risk group or International Prognostic Scoring System between CMML patients with RUNX1 mutations and those without mutations. The age of patients with mutations was younger than those without mutations (mean±s.e. 66.3±24 years vs 71.5±1.6 years, P=0.055). The percentage of BM blasts was 9.5±1.1% in patients with mutations compared with 7.1±0.7% in mutation-negative patients (P=0.073).
Sixteen of 30 RUNX1 mutation-positive CMML patients (53%) progressed to AML compared with 17 of 51 RUNX1 mutation-negative patients (33%) (P=0.102). We did not find a difference in the risk of AML transformation between CMML1 and CMML2 (P=0.146). The estimated 2-year AML transformation free was 21% for mutation-positive patients and 55% for mutation-negative patients. The overall survival of patients with RUNX1 mutations did not differ from those without mutations (median 15.7 months vs 12.8 months, P=0.928, Figure 2a). The median time to AML transformation in patients with RUNX1 mutations was 23.3 months compared with 28.3 months for those without RUNX1 mutations (P=0.175, Figure 2b). For patients with RUNX1 mutations, no significant difference was observed between the locations of mutations (N-terminal vs C-terminal) or between the patterns of mutations (missense vs frameshift/nonsense) with respect to the clinicohematological features, overall survival or time to AML transformation (Supplementary Table 2). Nine of the 21 patients (43%) with N-terminal mutations had AML transformation compared with seven of nine patients with C-terminal mutations (P=0.118). Notably, we found that patients with C-terminal mutations had a significantly higher risk of AML transformation (P=0.023) and a shorter time to AML progression compared with mutation-negative patients (median 6.8 months vs 28.3 months, P=0.022, Figure 2b), whereas the N-terminal mutations had no influence on outcome (P=0.955 for overall survival and P=0.759 for time to AML progression).
RUNX1 mutations have been described in chemotherapy-related MDS, MDS of atomic bomb survivors or in de novo MDS,15, 16, 17, 18, 19 whereas RUNX1 mutations were rarely reported in CMML.19, 20, 25 To the best of our knowledge, mutations of RUNX1 have only been described in two patients with CMML. Imai et al.25 found that one of five CMML patients examined had V105X mutation and Nakao et al.19 reported that one out of eight patients with CMML had RUNX1 mutation. Harada et al.17 did not find RUNX1 mutations in four patients with CMML, nor did Preudhomme et al. in 27 CMML patients.26 In contrast to earlier studies, our result, which included the largest cohort of CMML patients who had no earlier history of chemotherapy or radiotherapy, showed for the first time a very high frequency of RUNX1 mutations, which was comparable with those reported in atomic bomb survivors or in chemotherapy-related MDS.15, 16
Earlier studies of RUNX1 mutations in CMML were carried out by the PCR-single-strand conformational polymorphism approach, and mainly focused on RHD (exons 3–5).19, 24, 25 We screened the whole cDNA of RUNX1b by direct sequencing for each of the three PCR products, which cover the entire coding sequences of RUNX1b. C-terminal mutations of RUNX1 were detected in nine patients with CMML; they would have been overlooked had the mutation analysis not included exons 7b and 8 of the RUNX1 gene. In this study, samples with abnormal sequencing results were subjected to repeated analyses by DNA PCR using alternative primer pairs to confirm the presence of unequivocal mutations. The low frequencies of RUNX1 mutations in the earlier studies were probably attributed to different patient populations along with the limited exons analyzed as well as the small number of CMML patients examined. Ethnic differences among cohorts might also be a reason for the distinct frequency of RUNX1 mutations between ours and earlier studies. However, we had examined a large number of Chinese patients with de novo AML and had found that RUNX1 mutations, except for AML-M0 subtype, were rarely present (data not shown).
Our results showed that 72% of RUNX1 mutations in CMML were located at the N-terminal region and 28% at the C-terminal region. C-terminal mutations have recently been described in a large series of MDS/AML in which no patient with CMML was included.17 Harada et al.17 observed that N-terminal mutations were highly associated with therapy-related MDS, whereas C-terminal mutations of RUNX1 preferentially occurred in de novo high-risk MDS or AML after antecedent de novo MDS. Our data showed that the locations of RUNX1 mutations in CMML patients were similar to those observed in therapy-related MDS/AML rather than in de novo MDS/AML. We also found that missense mutations of RUNX1 preferentially (89%) located in the RHD. On the other hand, frameshift or nonsense mutations were distributed throughout the entire RUNX1 gene in both N-terminal and C-terminal parts. Biallelic mutations were frequently observed in AML-M0 and in myeloid malignancies with acquired trisomy 21 and trisomy 13, but were rarely found in other leukemia subtypes.13, 20, 25, 26 Two of our CMML patients had biallelic heterozygous mutations, one carrying two missense mutations and the other carrying two frameshift mutations on separate alleles by cloning analysis. One patient each had trisomy 13 and trisomy 21 in the whole cohort of CMML patients; it is of note that both patients harbored RUNX1 mutations.
Thirteen patients (nos. 10–13 and 17–25) carrying N-terminal mutants resulting in frameshift and/or truncation of RUNX1 proteins in the middle of the RHD are expected to lose the ability of the RHD to bind to DNA sequences and lack transcriptional activities.13, 15, 20, 25, 27, 28 Five mutants (nos. 26–30) with frameshift mutations located in exons 7b or 8 producing stop codons downstream of the normal termination codon 453 are expected to retain the DNA-binding potential but to lose transactivation ability, and to act as dominant negative inhibitors of wild-type RUNX1, as described by Harada et al.17, 29One patient had a silent mutation at RHD (no. 9), which had been reported earlier;13 it is considered to have no effect on function. Two missense point mutations in the middle of RHD (Arg80Cys and Arg139Gly) were described earlier.13, 25 The remainder are six novel missense mutants at RHD (nos. 1–6) and one nonsense mutant, Arg293X, in three patients (nos. 14–16); whether these mutants can induce RUNX1 functional changes needs further investigation. The C-terminal missense mutation has not been reported earlier and all of the earlier identified C-terminal mutations resulted in frameshifts.17 Patient no. 8 had Pro398Leu, which is within the inhibitory domain (aa372-411) found to keep the full transactivating potential of RUNX1.30 Whether Pro398Leu has a decreased transactivating ability requires further study.
A 'two-hit' model of leukemogenesis of AML, that is, cooperation of class I mutations that drive proliferation and class II mutations that lead to differentiation block in leukemic cells, has been proposed.31 Activating mutations of FLT3 and RAS genes are of class I, whereas RUNX1 mutation is of class II. Our results showed that 16.7% of CMML patients with RUNX1 mutations had FLT3 or RAS mutations. Collaboration of other genetic or epigenetic alterations in the great majority of patients with CMML and RUNX1 mutations, especially at AML transformation, requires further investigation.
The mutational status of RUNX1 was also correlated with the clinicohematological characteristics in our patients with CMML at initial diagnosis. RUNX1 mutation-positive patients were younger and had a higher BM blast percentage than did mutation-negative patients, but the difference did not reach statistical significance. There were no significant differences in the clinicohematological features between RUNX1 mutation-positive and RUNX1 mutation-negative groups. Other investigators described that trisomy 13 and trisomy 21 were associated with RUNX1 mutations in AML.26, 32 Christiansen et al.16 found that RUNX1 mutations were significantly associated with monosomy 7 or 7q− in therapy-related MDS. In this study, trisomy 13 or trisomy 21 was only present in one each of those harboring RUNX1 mutations, whereas they were not detected in mutation-negative patients.
Harada et al.17 found that patients with de novo or secondary MDS/AML carrying RUNX1 mutations had a significantly poorer overall survival than those without mutations. Christiansen et al.16 observed that RUNX1 mutations in chemotherapy-related MDS were associated with a shorter time to AML transformation, and missense mutations had a shorter survival than those with frameshift or nonsense patterns. The prognostic relevance of RUNX1 mutations in CMML has not been determined earlier. We failed to find a survival difference between CMML patients with RUNX1 mutations and those without RUNX1 mutations. Patients with RUNX1 mutations had a trend of increased risk of AML transformation compared with that in mutation-negative patients, but the difference did not reach statistical significance. We also analyzed whether the locations and patterns of mutations had any clinical or prognostic effect on patients with CMML harboring RUNX1 mutations. We found that C-terminal mutations were associated with a significantly higher risk of AML transformation compared with those without mutations. Moreover, patients with C-terminal mutations progressed to AML more rapidly. The reason of rapid AML progression in patients with C-terminal mutation is unclear. We did not find the patterns of mutations, that is, missense or nonsense/frameshift, to have any influence on clinical outcome.
Accession codes
- 1
Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DA, Gralnick HR et al. Proposals for the classification of the myelodysplastic syndromes. Br J Haematol 1982; 51: 189–199.
- 2
Jaffe ES, Harris NL, Stein H, Vardiman JW . World Health Organization Classification of Tumors: Pathology & Genetics Tumors of Haematopoietic and lymphoid tissues. IARC Press: Lyon, 2001, pp 45–52.
- 3
Padua RA, Guinn BA, Al-Sabah AI, Smith M, Taylor C, Pettersson T et al. RAS, FMS and p53 mutations and poor clinical outcome in myelodysplasias: a 10-year follow-up. Leukemia 1998; 12: 887–892.
- 4
Hirsch-Ginsberg C, LeMaistre AC, Kantarjian H, Talpaz M, Cork A, Freireich EJ et al. RAS mutations are rare events in Philadelphia chromosome-negative/bcr gene rearrangement-negative chronic myelogenous leukemia, but are prevalent in chronic myelomonocytic leukemia. Blood 1990; 76: 1214–1219.
- 5
Cross NC, Reiter A . Tyrosine kinase fusion genes in chronic myeloproliferative diseases. Leukemia 2002; 16: 1207–1212.
- 6
Shih LY, Huang CF, Wang PN, Wu JH, Lin TL, Dunn P et al. Acquisition of FLT3 or N-ras mutations is frequently associated with progression of myelodysplastic syndrome to acute myeloid leukemia. Leukemia 2004; 18: 466–475.
- 7
Shih LY, Lin TL, Wang PN, Wu JH, Dunn P, Kuo MC et al. Internal tandem duplication of fms-like tyrosine kinase 3 is associated with poor outcome in patients with myelodysplastic syndrome. Cancer 2004; 101: 989–998.
- 8
Shih LY, Huang CF, Lin TL, Wu JH, Wang PN, Dunn P et al. Heterogeneous patterns of CEBPα mutation status in the progression of myelodysplastic syndrome and chronic myelomonocytic leukemia to acute myeloid leukemia. Clin Cancer Res 2005; 11: 1821–1826.
- 9
Okuda T, van Deursen J, Hiebert SW, Grosveld G, Downing JR . AML1, the target of multiple chromosomal translocations in human leukemia, is essential for normal fetal liver hematopoiesis. Cell 1996; 84: 321–330.
- 10
Wang Q, Stacy T, Binder M, Marin-Padilla M, Sharpe AH, Speck NA . Disruption of the Cbfa2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis. Proc Natl Acad Sci USA 1996; 93: 3444–3449.
- 11
Asou N . The role of a Runt domain transcription factor AML1/RUNX1 in leukemogenesis and its clinical implications. Crit Rev Oncol Hematol 2003; 45: 129–150.
- 12
Speck NA, Gilliland DG . Core-binding factors in haematopoiesis and leukaemia. Nat Rev Cancer 2002; 2: 502–513.
- 13
Osato M, Asou N, Abdalla E, Hoshino K, Yamasaki H, Okubo T et al. Biallelic and heterozygous point mutations in the runt domain of the AML1/PEBP2alphaB gene associated with myeloblastic leukemias. Blood 1999; 93: 1817–1824.
- 14
Roumier C, Eclache V, Imbert M, Davi F, MacIntyre E, Garand R et al. M0 AML, clinical and biologic features of the disease, including AML1 gene mutations: a report of 59 cases by the Groupe Francais d'Hematologie Cellulaire (GFHC) and the Groupe Francais de Cytogenetique Hematologique (GFCH). Blood 2003; 101: 1277–1283.
- 15
Harada H, Harada Y, Tanaka H, Kimura A, Inaba T . Implications of somatic mutations in the AML1 gene in radiation-associated and therapy-related myelodysplastic syndrome/acute myeloid leukemia. Blood 2003; 101: 673–680.
- 16
Christiansen DH, Andersen MK, Pedersen-Bjergaard J . Mutations of AML1 are common in therapy-related myelodysplasia following therapy with alkylating agents and are significantly associated with deletion or loss of chromosome arm 7q and with subsequent leukemic transformation. Blood 2004; 104: 1474–1481.
- 17
Harada H, Harada Y, Niimi H, Kyo T, Kimura A, Inaba T . High incidence of somatic mutations in the AML1/RUNX1 gene in myelodysplastic syndrome and low blast percentage myeloid leukemia with myelodysplasia. Blood 2004; 103: 2316–2324.
- 18
Steensma DP, Gibbons RJ, Mesa RA, Tefferi A, Higgs DR . Somatic point mutations in RUNX1/CBFA2/AML1 are common in high-risk myelodysplastic syndrome, but not in myelofibrosis with myeloid metaplasia. Eur J Haematol 2005; 74: 47–53.
- 19
Nakao M, Horiike S, Fukushima-Nakase Y, Nishimura M, Fujita Y, Taniwaki M et al. Novel loss-of-function mutations of the haematopoiesis-related transcription factor, acute myeloid leukaemia 1/runt-related transcription factor 1, detected in acute myeloblastic leukaemia and myelodysplastic syndrome. Br J Haematol 2004; 125: 709–719.
- 20
Osato M . Point mutations in the RUNX1/AML1 gene: another actor in RUNX leukemia. Oncogene 2004; 23: 4284–4296.
- 21
Greenberg P, Cox C, LeBeau MM, Fenaux P, Morel P, Sanz G et al. International scoring system for evaluating prognosis in myelodysplastic syndromes. Blood 1997; 89: 2079–2088.
- 22
Shih LY, Huang CF, Wu JH, Lin TL, Dunn P, Wang PN et al. Internal tandem duplication of FLT3 in relapsed acute myeloid leukemia: a comparative analysis of bone marrow samples from 108 adult patients at diagnosis and relapse. Blood 2002; 100: 2387–2392.
- 23
Hopfner KP, Eichinger A, Engh RA, Laue F, Ankenbauer W, Huber R et al. Crystal structure of a thermostable type B DNA polymerase from Thermococcus gorgonarius. Proc Natl Acad Sci USA 1999; 96: 3600–3605.
- 24
Song WJ, Sullivan MG, Legare RD, Hutchings S, Tan X, Kufrin D et al. Haploinsufficiency of CBFA2 causes familial thrombocytopenia with propensity to develop acute myelogenous leukaemia. Nat Genet 1999; 23: 166–175.
- 25
Imai Y, Kurokawa M, Izutsu K, Hangaishi A, Takeuchi K, Maki K et al. Mutations of the AML1 gene in myelodysplastic syndrome and their functional implications in leukemogenesis. Blood 2000; 96: 3154–3160.
- 26
Dicker F, Haferlach C, Kern W, Haferlach T, Schnittger S . Trisomy 13 is strongly associated with AML1/RUNX1 mutations and increased FLT3 expression in acute myeloid leukemia. Blood 2007; 110: 1308–1316.
- 27
Imai O, Kurokawa M, Izutsu K, Hangaishi A, Maki K, Ogawa S et al. Mutational analyses of the AML1 gene in patients with myelodysplastic syndrome. Leuk Lymphoma 2002; 43: 617–621.
- 28
Michaud J, Wu F, Osato M, Cottles GM, Yanagida M, Asou N et al. In vitro analyses of known and novel RUNX1/AML1 mutations in dominant familial platelet disorder with predisposition to acute myelogenous leukemia: implications for mechanisms of pathogenesis. Blood 2002; 99: 1364–1372.
- 29
Harada H, Harada Y . Point mutations in the AML1/RUNX1 gene associated with myelodysplastic syndrome. Crit Rev Eukartyot Gene Expr 2005; 15: 183–196.
- 30
Kanno T, Kanno Y, Chen LF, Ogawa E, Kim WY, Ito Y . Intrinsic transcriptional activation-inhibition domains of the polyomavirus enhancer binding protein 2/core binding factor alpha subunit revealed in the presence of the beta subunit. Mol Cell Biol 1998; 18: 2444–2454.
- 31
Kelly LM, Gilliland DG . Genetics of myeloid leukemias. Annu Rev Genomics Hum Genet 2002; 3: 179–198.
- 32
Preudhomme C, Warot-Loze D, Roumier C, Grardel-Duflos N, Garand R, Lai JL et al. High incidence of biallelic point mutations in the Runt domain of the AML1/PEBP2 alpha B gene in Mo acute myeloid leukemia and in myeloid malignancies with acquired trisomy 21. Blood 2000; 96: 2862–2869.
This study was supported by grants NHRI-EX95-9434SI, NHRI-EX96-9434SI and NHRI-EX97-9711SI from the National Health Research Institute (L-Y Shih), and grant MMH-E-97009 from the Mackay Memorial Hospital (D-C Liang). We thank Dr Po-Nan Wang and Dr Po Dunn for providing patient samples and Ms Yu-Feng Wang for secretarial assistance.
Additional information
Supplementary Information accompanies the paper on the Leukemia website (http://www.nature.com/leu)
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Cite this article
Kuo, M., Liang, D., Huang, C. et al. RUNX1 mutations are frequent in chronic myelomonocytic leukemia and mutations at the C-terminal region might predict acute myeloid leukemia transformation. Leukemia 23, 1426–1431 (2009) doi:10.1038/leu.2009.48
Issue Date
- RUNX1 mutation
- AML1 mutation
- chronic myelomonocytic leukemia
- AML transformation
- C-terminal mutation
Further reading
RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia
Journal of Hematology & Oncology (2019)
Clonal Hematopoiesis with Oncogenic Potential (CHOP): Separation from CHIP and Roads to AML
International Journal of Molecular Sciences (2019)
DDX41 mutations in myeloid neoplasms are associated with male gender, TP53 mutations and high‐risk disease
American Journal of Hematology (2019)
Defining the Boundary Between Myelodysplastic Syndromes and Myeloproliferative Neoplasms
Surgical Pathology Clinics (2019)
Chronic Myelomonocytic Leukemia: Insights into Biology, Prognostic Factors, and Treatment
Current Oncology Reports (2019)


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Tevin Coleman
2019 Rush/Rec Stats
2019 Rush/Rec Projections
2019 Fantasy Outlook
Second verse, same as the first. Last year, coach Kyle Shanahan brought in Jerick McKinnon, a fast, athletic back who was comfortable working in a timeshare. Shanahan did it again this offseason when he brought in Coleman (a fast, athletic back who's comfortable working in a timeshare) from his old Atlanta stomping grounds. The former Falcon earned his free-agent payday by posting career highs in rushing yards and YPC and playing 16 games for the first time in 2018. His best trait is his blistering speed in open space, and Shanahan's system is perfect for getting him the ball with plenty of green in front of him. In his last season under his old/new coach, Coleman averaged an eye-popping 10.5 yards per target, a figure that ranked sixth in the league among all players (not just running backs). While he could be a big-play weapon again with the Niners, how many touches Coleman gets is a difficult question. McKinnon could be available at some point this season, and Matt Breida has shown the last two years he's a good fit for the Shanahan offense as well. Read Past Outlooks
$Signed a two-year, $8.5 million contract with the 49ers in March of 2019.
More competition for reps
Coleman is welcoming back Matt Breida (ankle) to the 49ers' backfield Sunday at New Orleans, Matt Maiocco of NBC Sports Bay Area reports.
Coleman received his normal workload in Weeks 11 and 12 en route to 28 touches for 126 yards from scrimmage and one TD. In Week 13 at Baltimore, though, Coleman was usurped by Raheem Mostert, seeing just six touches to the latter's 21. The reintroduction of Breida further clouds Coleman's potential to produce. especially against a Saints defense that has allowed just 3.5 yards per carry and 97.6 yards from scrimmage per game to running backs in 12 outings this season.
Coleman received his normal workload in Weeks 11 and 12 en route to 28 touches for 126 yards from scrimmage and one TD. In Week 13 at Baltimore, though, Coleman was usurped by Raheem Mostert, seeing just six touches to the latter's 21. The reintroduction of Breida further clouds Coleman's potential to produce. especially against a Saints defense that has allowed just 3.5 yards per carry and 97.6 yards from scrimmage per game to running backs in 12 outings this season.
NFL Stats
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Fantasy/Red Zone Stats
See red zone opportunities inside the 20, 10 and 5-yard lines along with the percentage of time they converted the opportunity into a touchdown.
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Advanced NFL Stats
How do Tevin Coleman's 2019 advanced stats compare to other running backs?
This section compares his advanced stats with players at the same position. The bar represents the player's percentile rank.The longer the bar, the better it is for the player.
- Broken Tackle %The number of broken tackles divided by rush attempts.
- Positive Run %The percentage of run plays where he was able to gain positive yardage.
- % Yds After ContactThe percentage of his rushing yards that came after contact.
- Avg Yds After ContactThe average rushing yards he gains after contact.
- Rushing TD %Rushing touchdowns divided by rushing attempts. In other words, how often is he scoring when running the ball.
- Touches Per GameThe number of touches (rushing attempts + receptions) he is averaging per game
- % Snaps w/TouchThe number of touches (rushing attempts + receptions) divided by offensive snaps played.
- Air Yards Per GameThe number of air yards he is averaging per game. Air yards measure how far the ball was thrown downfield for both complete and incomplete passes. Air yards are recorded as a negative value when the pass is targeted behind the line of scrimmage. All air yards data is from Sports Info Solutions and does not include throwaways as targeted passes.
- Air Yards Per SnapThe number of air yards he is averaging per offensive snap.
- % Team Air YardsThe percentage of the team's total air yards he accounts for.
- % Team TargetsThe percentage of the team's total targets he accounts for.
- Avg Depth of TargetAlso known as aDOT, this stat measures the average distance down field he is being targeted at.
- Catch RateThe number of catches made divided by the number of times he was targeted by the quarterback.
- Drop RateThe number of passes he dropped divided by the number of times he was targeted by the quarterback.
- Avg Yds After CatchThe number of yards he gains after the catch on his receptions.
Broken Tackle %8.3%
Positive Run %76.7%
% Yds After Contact57.0%
Avg Yds After Contact2.2
Rushing TD %5.0%
Touches Per Game14.0
% Snaps w/Touch43.3%
Air Yards Per Game-3.1
Air Yards Per Snap-0.10
% Team Air Yards-1.3%
% Team Targets7.7%
Avg Depth of Target-1.1 Yds
Catch Rate74.1%
Drop Rate3.7%
Avg Yds After Catch10.0
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NFL Game Log
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Half PPR
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Snap Distribution / Depth Chart
49ers 2019 RB Snap Distribution See more data like this
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Receiving Alignment Breakdown
See where Tevin Coleman lined up on the field and how he performed at each spot.
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This Week's Opposing Pass Defense
How does the Saints pass defense compare to other NFL teams this season?
The bars represents the team's percentile rank (based on QB Rating Against). The longer the bar, the better their pass defense is. The team and position group ratings only include players that are currently on the roster and not on injured reserve. The list of players in the table only includes defenders with at least 3 attempts against them.
@ Saints
Sunday, Dec 8th at 1:00PM
Overall QB Rating Against76.0
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2019 Tevin Coleman Split Stats
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Measurables Review View College Player Page
How do Tevin Coleman's measurables compare to other running backs?
This section compares his draft workout metrics with players at the same position. The bar represents the player's percentile rank. For example, if the bar is halfway across, then the player falls into the 50th percentile for that metric and it would be considered average.
* The 40-Yard Dash metric is from his Pro Day. All others are from the NFL Combine.
Height6' 1"
Weight210 lbs
40-Yard Dash*4.40 sec
Bench Press22 reps
Hand Length8.63 in
Arm Length32.00 in
Recent RotoWire Articles Featuring Tevin Coleman
Erik Siegrist previews Week 14 in the NFL as Russell Wilson and the Seahawks travel to Los Angeles to face the NFC West rival Rams.
Jerry Donabedian picks out the best and worst matchups for Week 14, including a doozy for Falcons running back Devonta Freeman.
Vikings running back Delvin Cook left Monday night's game with a shoulder and chest injury but downplayed the injury following the game.
Kevin Payne analyzes the top waiver-wire pickups for Week 14 as Jacoby Brissett has the matchup of the week against a bad Buccaneers defense.
Jerry Donabedian breaks down all the NFL action from Week 13, including Buffalo's continued success utilizing a no-huddle offense.
Past Fantasy Outlooks
Coleman ran for a career-high 628 yards in 2017, but it's tough to rate his overall performance as a step forward. His 4.0 YPC was a career low, and his receiving numbers also took a slight tumble as new offensive coordinator Steve Sarkisian failed to put Coleman in a position to use his explosive speed in the open field as often as Kyle Shanahan had. Heading into his fourth NFL season, Coleman still hasn't quite established that he's capable of being a lead back, after getting mixed results last season while Devonta Freeman was sidelined. Coleman's wheels are undeniable, but he lacks the vision or elusiveness to make the best use of his straight-line speed, and despite a solid frame he doesn't run with a lot of strength or physicality, resulting in fewer broken tackles than top-tier running backs create. He did at least play in a career-high 15 games last season, mostly avoiding the minor injuries that cut into his first two NFL campaigns. With Freeman still getting most of the snaps and touches in Atlanta's backfield, Coleman is unlikely to engineer a breakout season in the final year of his rookie contract unless his teammate misses significant time.
The third-year back had a breakthrough performance in 2016, scoring 11 total touchdowns on only 149 touches as he emerged as one of the Falcons' big-play options during their Super Bowl run. Coleman's game-breaking speed in the open field his primary asset, as he still flashes the 4.44 40-yard time he recorded at the 2015 Combine, and his offseason work to become a more complete back also paid off with a big increase in his role as a pass catcher. After catching only two passes as a rookie, Coleman hauled in 31 of 40 targets and used that speed to pile up 13.6 yards per catch. Unfortunately, health issues once again kept him from playing a full schedule, as a hamstring strain cost the 24-year-old three games in the middle of the season. Despite his 6-1, 210-pound frame, Coleman isn't a particularly physical runner, and his skill set is more of a match for Devonta Freeman's than a complement, but that duplication was used very effectively by offensive coordinator Kyle Shanahan in 2016 as he kept defenses from guessing which back would get which assignment on any particular play. Whether new coordinator Steve Sarkisian can keep that approach intact will go a long way towards determining whether Coleman can repeat, or even improve upon, last year's production.
When camps opened last summer, Coleman was viewed as the front-runner in the Atlanta backfield, with Devonta Freeman the secondary option. Coleman was the starter on opening night, but a rib injury knocked him back and Freeman wound up taking the job and running with it. Coleman didn't help his own cause with some occasional fumbling issues. This doesn't mean things can't flip in 2016. Coleman averaged a solid 4.5 per carry as a rookie and Freeman's play dipped significantly in the second half of the year. We know any starting back in a Kyle Shahahan offense can be a bundle of fun, which means at minimum, Coleman is one of the highest-upside backups in the league. Keep an eye on him.
While first-round picks Todd Gurley and Melvin Gordon are no slouches, Coleman could be the most exciting back selected in this year's draft, as he can turn any touch into a touchdown. In fact, half of his 28 career rushing TDs at Indiana came on runs of 40 or more yards. While he doesn't have ideal size for a feature back at 5-11, 206, Coleman is incredibly explosive, hitting holes and getting through to the second level before the defense knows what hit it, and his 4.39 time in the 40-yard dash isn't just a track mirage. He also flashes great elusiveness, slipping tackles and changing direction without slowing, and when he does finally run out of room he isn't afraid to lower his pads and fight for extra yards. His receiving skills are also solid, but Coleman's ability to outrun everyone in college did mask some deficiencies, and his vision and patience will need some work for him to take full advantage of his gifts in the NFL. Second-year back Devonta Freeman, a similarly slippery but undersized runner, has the incumbent's advantage in a training camp battle for the top spot in the backfield, and holdover Antone Smith will also see some touches, but Coleman's game-changing speed should make it difficult to keep him off the field.
More Fantasy News
Cedes most work to Mostert
Coleman rushed five times for six yards and caught his lone target for nine more in Sunday's 20-17 loss to the Ravens.
Score saves mediocre day
Coleman rushed 11 times for 39 yards and a touchdown while catching two passes (four targets) for 10 yards in Sunday's 37-8 win over the Packers.
Bottled up in win
Coleman carried the ball 12 times for 14 yards while catching three of four targets for 48 yards in Sunday's 36-26 win over the Cardinals.
Returns to practice
Coleman (personal) was on the field for Friday's practice, Cam Inman of The San Jose Mercury News reports.
Absence not related to injury
Coleman (personal) was excused from Thursday's practice due to a personal matter, Matt Barrows of The Athletic reports.


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Franchise Group (NYSE:FRG) declared a dividend on Monday, November 25th, Fidelity reports. Investors of record on Friday, December 6th will be given a dividend of 0.25 per share on Monday, January 6th. The ex-dividend date is Thursday, December 5th.
NYSE FRG opened at $14.00 on Wednesday. Franchise Group has a 52 week low of $11.92 and a 52 week high of $14.00.
Separately, TheStreet lowered shares of Franchise Group from a "c" rating to a "d+" rating in a report on Friday, November 15th.
Franchise Group, Inc, through its subsidiaries, provides tax preparation services in the United States and Canada. The company also facilitates refund-based tax settlement financial products, such as refund transfer products and personal income tax refund discounting, as well as provides an online digital Do-It-Yourself tax program in the United States.
Further Reading: Forex
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Openly Gay Richard Quest is apparently getting a very warm welcome from a homophobic Nigeria
CNN anchor, Richard Quest, is in Nigeria to conduct some interviews for his program, Quest Means Business. Quest is also an openly gay man, and has received nothing but the warmest reception since arrival in Nigeria.
Reacting to this, Gay rights activist Bisi Alimi, in a post on Instagram called Nigerians hypocrites for according Quest the respect they would not their fellow countrymen who are gay.
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17 Comments
They celebrate out of ignorance..
They are celebrating him because of achievements not because of his sexuality.
of course they will celebrate him, Nigerians are obsessed with anything oyibo or foreign. At this point, nobody cares if he's gay or not.
When you worship a white God what do you expect?
Moral of the story when you gay, rich and powerful, most Nigerians no send. Selfie. ??
Nigerians are so gullible dats if the word applies.
How to be gay in Nigeria? Be rich and very successful!
I mean it's fucking annoying. But obviously our inferiority complex won't allow us be great. These white people do not see us as equals but we practically worship them (a white man would come down to our country and make one of us his driver *rme*).
It's obviously with this mindset that Richard is given the strawberry treatment and our very own gay personalities are regarded abominations.
Such mind-boggling shame.
What would you have preferred?? Him being stoned and chased out of Nigeria?? Or being imprisoned? Let him enjoy his stay here instead of looking for ways to tarnish him. And who knows If he is being judged behind the scenes!
Don't u guys think saying anything now is smehw risky… This might make Nigerian homophobes wanna prove themselves…. Sha they might not wanna try themselves with d US…eh..ehnnn…when dem no wan die…But this one Trump is president….hmmmn…. Sha all am saying is it wud b beta if dis gist came up after he's left.
It's not just about sexuality, it's a lot of things. I schooled in Benin Republic & you should see the unity amongst their people. Over there, see Nigerians fighting, criticizing & even killing their own. I'd never forget this one experience I had that made me realize there's just no hope for the people. Guess what, the government we have have absolutely nothing to do with our bloody bigotry.