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At One Stone Events, you will receive a connected customer service experience.
I design your event with heart, and I do it with style.
a little modern, a little rustic, a lot whimsical!
- Weddings
- Corporate Events
- Mitzvahs
- Quinceaneras
- Birthday parties for all ages
- Bridal showers
- Baby showers
- Sip N Sees
- Gender reveal parties
- Anniversary celebrations
- Engagement parties
- Rehearsal Dinners
- Christenings
- Baby Namings
- Everyday celebrations
- Dessert tables or candy buffets
- And more!…
For more information on having One Stone Events design your wedding, corporate event or party, please contact Elissa Stone by clicking here!
What you get when you hire a designer:
Event Designer: The one who creates the look and feel for your event, encompasses your personality, comes up with unique ideas and details and 'wows' your guests.
Event Planner: The one who coordinates logistics, hires vendors, creates the timeline, manages the event and puts out fires.
At One Stone Events, my specialty is DESIGN.
When you hire me, you're getting someone with a passion for making spaces beautiful. I have a unique and creative style and my vision is what sets me apart. If you want someone who will execute the fine details and provide a unique experience for your guests, you need a designer.
If you want someone to hire your vendors and manage your event, then a planner is for you. Planning packages are available in addition to our design services.

subset: commoncrawl


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Rendering of A330-300 in Delta Air Lines' livery
Courtesy, Airbus
Delta Air Lines has placed a firm order for 40 Airbus aircraft comprising 10 A330-300 and 30 A321ceos for delivery between 2015 and ...
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Global Free Space Optics Market Report, published by Variant Market Research, forecast that the global market is expected to reach $1223.1 Million by 2024 from $101.9 Million in 2016, growing at a CAGR of 36.4% from 2016 to 2024. By geography, Asia Pacific and North America are expected to grow at a CAGR of 38.1% and 36%, respectively, during the forecast period.
"Free Space Optics Market (By Component: Transmitter, Receiver, Modulator, Demodulator, Encoder & Decoder And Others; By Application: Security, Airborne Application, Healthcare, Storage Area Network, Data Transmission, Defense, Disaster Recovery, Last Mile Access And Others; By Geography: North America, Europe, Asia-Pacific and RoW) Global Scenario, Market Size, Outlook, Trend and Forecast, 2015-2024"
To browse the complete report, visit at https://www.variantmarketresearch.com/report-categories/information-communication-technology/free-space-optics-market
The ever expanding telecommunication industry and rising demand for data streaming and multimedia service fuels the growth of the market. Moreover, increasing applications of free space optics into military and healthcare and high penetration of 3G and 4G in emerging economies also boosts the growth of the free space optics market.
By component segment, transmitter and receiver accounted for 28.6% and 26.7% respectively, in the year 2016. Moreover, in applications, data transmission holds the highest share of 21.52% due to high penetration of internet, followed by defense with 20.39%, due to increasing budget allocation for the military.
Asia Pacific is the fastest growing region in the global Free Space Optics market is expected to continue this trend due to increasing expenditure by government, heavy investments on infrastructure, development of smart cities and emergence of technology start-up companies. North America accounts the largest share of the market followed by Europe in the year 2016.
The key market players include Panasonic Corp., Trimble Hungary Ltd., Anova Technologies, Wireless Excellence Ltd., General Electronics Co., Fujitsu Ltd., Lightbee Corp., Harris Corporation, Outstanding Technology, LightPointe Communications, Inc., pureLiFi Ltd., and Koninklijke Philips N.V. among others.
Contact Us:
John Dave,
Head - Sales
Variant Market Research
649 Mission St, 5th Floor, San Francisco,
CA 94105,United States.
Tel: +91-953-444-8373
Email: firstname.lastname@example.com
Email: email@example.com
|Chapter 1 Prefix
|1.1 Market Scope
|1.2 Report Description
|1.3 Research Methodology
|1.3.1 Primary Research
|1.3.2 Secondary Research
|1.3.3 In-house Data Modeling
|Chapter 2 Executive Summary
|Chapter 3 Market Outline
|3.1 Market Inclination, Trend, Outlook and Viewpoint
|3.2 Market Share Analysis: Company's Competitive Scenario
|3.3 Value Chain Analysis
|3.4 Market Dynamics
|3.4.1 Drivers
|188.8.131.52 Impact Analysis
|3.4.2 Restraints
|184.108.40.206 Impact Analysis
|3.4.3 Opportunities
|3.5 Porter's five forces analysis
|3.5.1 Factors Impact Analysis
|Chapter 4 Free Space Optics Market by Component Type: Market Size and Forecast, 2015 – 2024
|4.1 Transmitter
|4.1.1 Current Trend and Analysis
|4.1.2 Market Size and Forecast
|4.2 Receiver
|4.2.1 Current Trend and Analysis
|4.2.2 Market Size and Forecast
|4.3 Modulator
|4.3.1 Current Trend and Analysis
|4.3.2 Market Size and Forecast
|4.4 Demodulator
|4.4.1 Current Trend and Analysis
|4.4.2 Market Size and Forecast
|4.5 Encoder & Decoder
|4.5.1 Current Trend and Analysis
|4.5.2 Market Size and Forecast
|4.6 Others
|4.6.1 Current Trend and Analysis
|4.6.2 Market Size and Forecast
|Chapter 5 Free Space Optics Market by Applications: Market Size and Forecast, 2015 – 2024
|5.1 Security
|5.1.1 Current Trend and Analysis
|5.1.2 Market Size and Forecast
|5.2 Airborne Application
|5.2.1 Current Trend and Analysis
|5.2.2 Market Size and Forecast
|5.3 Healthcare
|5.3.1 Current Trend and Analysis
|5.3.2 Market Size and Forecast
|5.4 Storage Area Network
|5.4.1 Current Trend and Analysis
|5.4.2 Market Size and Forecast
|5.5 Data Transmission
|5.5.1 Current Trend and Analysis
|5.5.2 Market Size and Forecast
|5.6 Defense
|5.6.1 Current Trend and Analysis
|5.6.2 Market Size and Forecast
|5.7 Disaster Recovery
|5.7.1 Current Trend and Analysis
|5.7.2 Market Size and Forecast
|5.8 Last Mile Access
|5.8.1 Current Trend and Analysis
|5.8.2 Market Size and Forecast
|5.9 Others
|5.9.1 Current Trend and Analysis
|5.9.2 Market Size and Forecast
|Chapter 6 Free Space Optics Market by Geography: Market Size and Forecast, 2015 – 2024
|6.1 North America
|6.1.1 Current Trend and Analysis
|6.1.2 Market Size and Forecast
|6.1.4 Canada
|6.1.5 Mexico
|6.2 Europe
|6.2.1 Current Trend and Analysis
|6.2.2 Market Size and Forecast
|6.2.4 Italy
|6.2.5 France
|6.2.6 Germany
|6.2.7 Others
|6.3 Asia-Pacific
|6.3.1 Current Trend and Analysis
|6.3.2 Market Size and Forecast
|6.3.3 China
|6.3.4 India
|6.3.5 Japan
|6.3.6 South Korea
|6.3.7 Others
|6.4 RoW
|6.4.1 Current Trend and Analysis
|6.4.2 Market Size and Forecast
|6.4.3 Middle East
|6.4.4 South America
|6.4.5 Africa
|Chapter 7 Company Profiles
|7.1 Panasonic Corp.
|7.2 Trimble Hungary Ltd.
|7.3 Anova Technologies
|7.4 Wireless Excellence Ltd.
|7.5 General Electronics Co.
|7.6 Fujitsu Ltd.
|7.7 Lightbee Corp.
|7.8 Harris Corporation
|7.9 Outstanding Technology
|7.10 LightPointe Communications, Inc.
|7.11 pureLiFi Ltd.
|7.12 Koninklijke Philips N.V.
Please fill the form below and our research expert will get in touch with you.
Please fill the form below and our research expert will get in touch with you.


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Historic House in Yonkers Demolished while preservation legislation is considered
From Chuch Lesnick, Yonkers City Council President
Real Estate Committee Chair, Dennis Robertson, and I (Real Estate Co-Chair) have scheduled a meeting for Tuesday, August 14, 2007 at 7:30 PM in the Council Chambers, City Hall, Fourth Floor. We will be discussing a local law that was introduced in Rules on June 5th to amend the Code of the City of Yonkers, in relation to requiring review by the Landmarks Preservation Board before a demolition permit may be issued for historically significant buildings.
Historic Preservation is very important to the City of Yonkers because the older buildings often define our community. While I don't believe that every building should be saved just because it is old, we do believe that the City of Yonkers must allow its Landmark and Historic Preservation Board to do what it is supposed to do — look at each and every property of perhaps 75 years and older (to be decided) and make a determination as to whether or not the property ought to be landmarked before any demolition permits are issued.
This law may be the most historic preservation legislation that the Council has considered this century. Please come and support it.
The following excerpt about the Smith Collins House was taken from LANDMARKS LOST & FOUND: An Introduction to the Architecture and History of Yonkers:"While both Gothic styles employ many of the same motifs, the High Victorian Gothic is distinguished from its predecessor by the different fashion in which its details are treated.
Illustrating this difference is the Smith-Collins House at 323 Palisade Avenue. The lacy vergeboards of the earlier period are here replaced by strong, massive members with heavily molded profiles, suiting the Victorian notion of "character," while its interconnecting braces despite their merely ornamental function resemble structural elements and answer the call for "truthful" architecture. Furthermore, its roofscape is much more complicated than that which would have been found on an earlier Gothic Revival building — it employs not only cross-gables but the large, hipped roofs that are a characteristic of many High Victorian Gothic buildings. The Smith-Collins House is a particularly interesting building which seems to have undergone a number of alterations over its time.
Featuring the corner pilasters and linteled windows that are usually associated with late Georgian and early Federal buildings, the house also incorporates a tall tower whose round arched windows are Italianate in inspiration. Appearing on 1854 tax records as the residence of L.F. Wheeler, the house was annexed to the adjacent Alexander Smith estate in 1873, and evidence suggests that shortly thereafter it was remodeled in the then fashionable High Victorian Gothic style.
Despite a lack of documentary evidence, it is posited that the building was the residence of Warren B. Smith, Alexander Smith's eldest son, until his father's death in 1878. In that year, Warren Smith, who succeeded to the presidency of the family's carpet mills, took up residence at the Smith estate "Hillbright" and the house was sold to Charles Collins, a successful dry goods merchant from Hartford, Conn. who had moved to Yonkers to retire. In 1910, the dwelling was conveyed to Isidor J. Beaudrias, a prominent local lawyer. His application of stucco to its clapboard siding, remodeling of many of its porches, and removal of most of its decorative trim converted it into the castle-like structure that stands today."
Not withstanding the pending legislation, the City administration issued a demolition permit for the Historic Smith Collins House. Parts of this house apparently date back to the 1850's. I was going to use this space to rally public support to save the building — but we are too late!


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The Rising Tide of Anti-Semitism and the Persecution of Christians
Posted in PJC, SFSMultimedia, PJCMultimedia, PJCAudio, SFSAudio on Mar 26th, 2015 Comments
Ambassador Ronald S. Lauder presented this year's Herman Allen "Hal" Israel (C'92) Endowed Lectureship in Jewish-Catholic Relations on "The Rising Tide of Anti-Semitism and the Persecution of Christians" (March 25, 2015).
Ambassador Lauder is the current President of the World Jewish Congress (WJC). As President of the WJC, Ambassador Lauder has met with countless heads of state, prime ministers, and government representatives in advancing those causes that are of most concern to Jews and Jewish communities internationally.


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Tests of NT-3 gene therapy on mouse models suggest potential to treat a host of muscle-wasting conditions.
A gene known to repair damaged nerves also repairs damaged muscle, a team of investigators at Nationwide Children's Hospital discovered.
The gene neurotrophin 3, or NT-3, increases muscle fiber diameter and induces remodeling of fiber types, the researchers found. The result is the size and composition of muscle fibers in a mouse model of diseased neurogenic muscle change to mirror the size and composition found in healthy wild-type mice. The therapy was tested in a mouse model of Charcot-Marie-Tooth disease (CMT), an inherited neurological disorder affecting the limbs.
The gene triggers the mTORC1 cell signaling pathway promoting muscle survival and growth, in vivo and in vitro experiments showed. Delivered to healthy muscle, NT-3 had no observable effect, indicating the gene is geared toward repair, the researchers say. They report their findings in Gene Therapy.
The findings suggest NT-3 has the potential to treat not only neuropathies to benefit nerves and muscles, the research team says, but also treat muscles in muscle-wasting conditions such as aging, cancer cachexia, certain types of muscle atrophy and genetic or acquired autoimmune primary muscle disorders.
Simply put, "When muscle fiber diameter increases, force generation improves," says Zarife Sahenk, MD, PhD, a principal investigator in the Center for Gene Therapy in The Research Institute at Nationwide Children's and leader of the research.
Dr. Sahenk's lab has been studying NT-3 for more than a decade, and its recent gene therapy experiments treating mouse models of neuropathy showed the treatment improved nerve regeneration and myelination and increased muscle fiber diameter.
In this study, 16 weeks after delivering NT-3 to muscle via adeno-associated virus 1, the muscle diameter increases were greatest in fast-twitch glycolytic (FTC), also called the type IIb muscle fiber subtype, and now equaled the diameter in healthy mice. Prior to delivery, the mouse model had fewer FTC fibers, proportionally, than wild-type mice. But measurements taken at 16 weeks showed fiber remodeling had normalized the proportion.
The changes in muscle were accompanied by significant increases in the levels of phosphorylated 4E binding protein and S6 protein in the treated muscle compared to the untreated — evidence of mTORC1 activation, the researchers say.
Initially, the lab thought the muscle may be responding to reinnervation. Dr. Sahenk's team tested the effect of NT-3 protein on muscle cultures without nerves. Through a series of experiments, they found direct evidence that NT-3 protein triggered protein synthesis by inducing the Akt/mTOR pathway in myotubes (a stage of muscle fiber development) and more evidence the gene was improving muscle and reversing the pathology.
Because of its versatility, Dr. Sahenk believes NT-3 therapy could be ideal for restoring damaged nerves and muscles in children who suffer chemotherapy-induced peripheral neuropathies, and a host of neural and muscle pathologies.
"These type II fibers are the first fibers to shrink due to inactivity," she says. "NT-3 could potentially counter the effects of aging on muscle, or be used in paraplegia of hemiplegia."
Yalvac ME, Amornvit J, Chen L, Shontz KM, Lewis S, Sahenk Z. AAV1.NT3 gene therapy increases muscle fiber diameter through activation of mTOR pathway and metabolic remodeling in a CMT mouse model. Gene Therapy. 2018 Mar 9. doi: 10.1038/s41434-018-0009-8. [Epub ahead of print]
Photo credit: Adobe Stock


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Sell My House Sandy Ridge, NC
Buy Houses In Sandy Ridge
Costs Leys, that performs specialized deck assessments for buyers in ###North Carolina:t#:t#:t#, claims the added prices for specialized assessments can conserve a customer tens of thousands in costs to fix concealed damages. The things he usually locates that house inspectors miss on decks, for instance, are improperly fastened railings, hazardous glazing anddry rot in difficult-to-access or concealed locations.
How Do I Sell My House Myself Sandy Ridge North Carolina7 amazing sell my house Sandy Ridge hacks.
Be certain to do so just after seeking advice from an attorney that is experienced in genuine estate issues if you want to revoke your offer. You do not want to lose your earnest money deposit or get sued for damages the seller could have endured by counting on your actions.
Whats your way of life? A house isn't simply a house. Its additionally a place and a way of living. You do not wish to make a decision that youll come to regret later. Another factor to consider to want is how much time you plan to be in the area or the residence. You might want to hold off getting a house till youre prepared if you have an even more transient way of life. Likewise, just how prepared are you for the responsibility of possessing a residence? Remember, youre the one that will need to supervise of its maintenance economically and also or else. If you are prepared to take this on, ask on your own.
Sell Your Home By Owner Sandy Ridge North Carolina
How you can Obtain A Home Mortgage With Bad Credit score Some financial institutions will make guaranteed financings based on the amount you have in a savings account or the worth of any kind of supplies you have. The value of obtaining a secured lending versus stocks or financial savings is that you will certainly not should sell off the asset so when you have actually settled the financing, you still own the cost savings or stocks. Nevertheless, if you prepare to make use of savings or stocks as security, many economic advisors suggest you liquidate them and utilize the money to pay whatever debt you are aiming to clear up rather than get a loan.
What To Offer On A House In Today's Market Seasonal Issues The general regulation is that the better the weather condition obtains particularly in locations with extreme winters the extra customers are around residence searching, driving up need. Conversely, no one actually wishes to relocate or market throughout the winter holidays, so the sellers whose residences are on the market normally have to move for some factor. High seller motivation can provide great possibilities for purchasers.
Quick Sale Home Sandy Ridge North Carolina
Getting bank possessed houses can be a large amount whether you intend to reside in the property, rent it out or flip it for a higher cost. When making deals on a REO, buyers need to not be daunted. These residential or commercial properties come with clear title as well as are without various other liens and also encumbrances. When the moment comes to get your bank owned confiscated home, simply make certain you are prepared to collaborate with the financial institution to get the very best offer possible.
Should I Sell My House Now in Sandy Ridge
Inspection of trees on the residential property is not considered component of a General House Examination due to the fact that their inspection exceeds the extent of examination Criteria of Technique. This type of assessment is normally carried out by a Qualified Arborist and examines the security and problem of the trees on a property prior to the sales contract is implemented.
Listing Your Home Sandy Ridge North Carolina
A CMA is the most widely made use of, reliable, and also mathematical method of estimating the true worth of "your" home, as well as functions similar to comparison shopping. A CMA distills your home right into its vital qualities namely, bedrooms, shower rooms, house as well as whole lot square video and also contrasts it with close-by residences that have similar features which are currently on the market or have recently marketed. Especially, your Real estate professional will get into MLS and look for residential or commercial properties:
Golden State Real Estate Investors Association http://www.realestateinvestorsassociation.org Get in touch with: Lon Hooker Telephone: (702) 735-6000 When: Last Wednesday of monthly, 7:00 pm Where: Sandy Ridge Country Club, Sandy Ridge.
House Not Selling What To Do in Sandy Ridge
House Evaluation List: Just What To Inspect Likewise see RESIDENCE & BUILDING INSPECTORS & ASSESSMENT TECHNIQUES. Utilize the Browse Box at the top or base of these pages to find extensive information about building, energy financial savings, and interior setting inspection, medical diagnosis as well as repair service at this internet site.
How Much To Sell Your House in Sandy Ridge
Finding A Realtor For Renting An excellent representative ought to find out about other location residential properties that are readily available off the top of his head, states Irwin. Point out a home in your location thats offered lately or is to buy. If the representative recognizes the residential or commercial property and also could give you a few details, that implies they really recognizes your area, he states. You desire somebody like that, whos in addition to the marketplace.
To Sell Your Home Fast Sandy Ridge North Carolina
Making A Deal On A House In France Attempt to understand the scenario from the sellers point of view. Place on your own in their footwear. Youll start to choose up on small information that will have a huge influence if you can do this. Keep in mind that every home is available for sale for a particular reason. Discover that reason, and also framework your deal in a way that makes use of it.
Wasatch Location Realty Investors Organization (WAREIA) http://www.wareia.com Call: Scott L. Moyes, President [email protected] Telephone: (801) 838-7989 When: 2nd Tuesday of every month, 6:30 pm Where: Area Libraries, Sandy Ridge.
Sell A House Fast Sandy Ridge North Carolina
Buying Mobile Homes in Sandy Ridge
Other articles that may be of interest to you:
- sell that home in Sandy Ridge, NC
- quick home sale in Sandy Ridge, NC
- how to sale your house fast in Sandy Ridge, NC
- sell my house in Sandy Ridge, NC
- buy homes in Sandy Ridge, NC


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Bass Strait cattle deaths investigation concludes
Bass Strait cattle deaths investigation concludes
RSPCA Tasmania advises that progress has been made in the investigation into the death of 59 cattle during a Bass Strait crossing aboard the MV Statesman in late January this year.
RSPCA Tasmania Inspectors have been assisting the Department of Primary Industries, Water and Environment (DPIPWE) with their investigation.
DPIPWE has concluded its investigation and is preparing a report to go to the Tasmanian Director of Public Prosecutions for consideration.
In the interim the MV Statesman continues to transport cattle across Bass Strait under strict animal welfare instructions from DPIPWE.


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Here's the latest video from local artist L.A.T.I., who is our friend (or whatever you're supposed to call a mutual connection) on Twitter -- you can follow her at @Latimusica (and us at @Crossfade_SFL). It's for "Spaceship," the lead single off her debut album, due out next year. Born LaTisha Marie Cotto, this Mexican-American songstress grew up in Waco, Texas and Atlanta, where she began to hone her sound as a singer, songwriter, and lyricist. You can hear a tiny bit of that city's influence in the mechanical, low-end grind of this song's beat.
If you like this story, consider signing up for our email newsletters.
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As we ring in 2011 tomorrow, I will be looking back on 2010 with a smile. I feel so lucky that it was a year filled with health and happiness for our family. It was also a year of special milestones. My husband and I celebrated 10 years of wedded bliss, our little princess finished Kindergarten, and our twin baby boys turned one. It was also the first year I stayed home in over a decade of working as a marketing professional, and I wouldn't change a thing. It allowed me to experience all the little things that matter so much.
So, here's to another very happy, healthy and successful year. Wishing everybody the very best for 2011!


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Vegetables sushi rolls are a very healthy and delicious alternative to a lot of the trans fat food we eat today. It has this delicious fresh crunch to it which makes you crave it even more. Follow along in these five cooking how-to videos to learn how to prepare, roll and cut a vegetable sushi roll. Learn how to cook the rice and mushrooms for your roll. The preparation of the sushi rice is a critical process because the rice gives the sushi its subtle texture and flavor.
Chinese long green beans
Green onions
Red and orange bell peppers
Basil leaves
Pickled radish


text: I wrote a post a while back listing ways anyone could make an extra $500 a month. Since then, I've been questioned about my strong belief that anyone can make more money if they choose to do so. I have never said earning extra money is easy. Even if whatever you're doing is not physically challenging, it takes time and energy that you could otherwise devote to things that are enjoyable. The good thing about extra income is that it doesn't have to mean working all the time forever. You might need to get some bills paid or build an emergency fund. After that goal is met, you can cut back IF you don't get addicted to the extra cash. In case you didn't like any of my ideas from the other post, here are 5 more ways anyone can make extra money. Adult Caregiver There are organizations that help take care of adults with mental disabilities. They work to train individuals for life and job skills and provide supervision if the client can't live alone. In our area, the organization is called Community Connections and they are always running ads for caregivers from 3PM to 8AM. You could take ...Read More »


text: This past weekend, Chrissy Teigen and John Legend tied the knot in Lake Como, Italy. Check out our exclusive photos from the intimate ceremony, to which the Sports Illustrated swimsuit model wore a custom Vera Wang gown (the first of three dresses she donned throughout the evening). The couple is now honeymooning in Portofino—but starting next week, Teigen will begin posting on ELLE.com as our blogger-in-residence. Stay tuned, and check out more exclusive images below.


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An enormous capital amount is required for setting up any new business or financing the capital enhancements. Liberty Capital Group: Liberty Capital Group gives all kinds of enterprise funding applications, including typical business loans, SBA loans, industrial actual estate loans, tools and machinery financing, traces of credit score, asset-based financing, and low-credit score financing.
The Southeast Asian Food and Agriculture Science and Technology venture, also called SEAFAST, has been aiding Indonesians with small- and medium-dimension food-based mostly enterprise enterprises since 2005, said Steven Gregory of Texas A&M's Borlaug Institute for International Agriculture.
The self-employed professionals like the Chattered Accountants, Medical doctors, Architects, legal professionals are additionally capable of take the benefit of these form of loans. This method permits the lender to shortly estimate and assess its return chance utilizing all the size of a enterprise and never just a credit rating. Whereas the perks and credit score limits won't be nearly as good as with a normal enterprise card, these cards may also help you enhance your credit rating (offered you pay on time) so you'll be able to qualify for even better financing in the future. Bond Street: Bond Avenue affords term loans starting from $50,000 to $500,000, with rates of interest beginning at 6 p.c and phrases from one to a few years.
The kinds of loans the company helps set up for small businesses embody small enterprise loans of up to $1 million, service provider money advances, equipment loans, factoring, 401(ok) rollover funding and features of credit. There are a number of financing options obtainable to veteran small business house owners, from authorities grants and loans to loans from marketplace lenders. Lending Club: Lending Club presents loans to most sorts of businesses, including skilled and private providers, retailers, contractors, health and wellness providers, automotive corporations, wholesalers, producers, and restaurants. Grameen America is able to spend money on ladies who don't have any credit score scores, no enterprise plans and can present no collateral. The lender approves advances to companies which have credit scores under 500 and makes funds obtainable to debtors within seventy two hours.
Capital for Merchants: Capital for Retailers is a enterprise cash-advance lender. Dealstruck: Dealstruck gives a wide range of small enterprise funding choices, including business term loans, revenue-secured term loans and asset-based mostly traces of credit. The lender makes use of customized know-how to supply small companies with fast, convenient and inexpensive enterprise loans. BFS Capital: BFS Capital provides both small business loans and merchant cash advances.


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Author: Chris Swann
Version: 220.127.116.11
Last Updated: 18-04-2017 15:22
Christianson, R., Wong, C., & McDonald, M. (2016). Upper Mississippi Nutrient (Loss) Reduction Strategies – Illinois, Iowa, and Minnesota (White Paper). Center for Watershed Protection and Walton Family Foundation. This document is intended to highlight similarities between nutrient reduction or nutrient loss reduction strategies for Illinois, Iowa, and Minnesota – focusing on agricultural BMPs. In addition, gaps in knowledge as well as focus areas for future research are suggested. As expected, there is substantial overlap in the BMP efficiencies developed by each state, which adds credibility to the independent efforts.


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There are good ideas and there are great ideas. For example...cheese - good idea. Cheese in a can - great idea! Well perhaps not everyone agrees with me on this. But most would agree that corporations should not get off scott-free when their products maim, injure or kill consumers. Corporate accountability - good idea. Actually introducing and enacting legislation that holds corporations accountable for faulty products that hurt consumers - great idea!
Thankfully, Congress moved one step closer to better protecting American consumers hurt by products from foreign manufacturers. Like the toys that contain excessive amounts of lead paint or the bad batches of the drug thinning drug, heparin. Today, U.S. House Judiciary Chairman John Conyers and Commercial and Administrative Law Subcommittee Chair Linda Sánchez put the cheese in a can so to speak by introducing the "Protecting Americans from Unsafe Foreign Products Act." In addition to Conyers and Sánchez, the bill has three other original co-sponsors. Sounds like a great idea to me.
I only hope the rest of Congress and the President realize the merit of this legislation. Corporations whether at home or abroad just like everyone else should be held accountable for the damage they cause. It just makes sense!


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Hello sunfish! I don't know about you but the weather lately has made me DESPERATELY wish for beach time. I'm not one to complain about snow and cold (I love it), but this year it feels like it has lingered. Warm weather needs to step in and take over.
So, to pay homage to the sweltering days that lie ahead, lets dive in (see what I did there?) and take a look at some cool things to bring to the beach! P.S-none of this is sponsored (HINT #$@#^%&##@$@# HINT ).


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You can add honey and milk and eat cracked wheat cereal for breakfast, or you can use it when making delicious
seafood salad.
or in your
homemade chili.
by Melonie Turley
Boil water in pan. Add wheat and bring to boil. Remove from heat and cover. Let it sit for 30 minutes until water is absorbed or simmer for 20 minutes. (Vitamins and flavor are retained when foods are not boiled.)
Freeze in 2 cup portions in Ziploc bags for easy use later.
Food Storage Recipes from Cracked Wheat Cereal
Healthy Breakfast Foods
Wheat Recipes


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Poems (Coates 1916)/Volume II/An Idler
For other versions of this work, see An Idler.
SHE cannot wind the distaff,
SHE cannot wind the distaff,
She can nor bake nor brew;
Her hands are indeed too dainty
Such labors to pursue.
She cares not to follow the harvest,
She neither can sew nor glean,
But waits for the weary reapers
With cheerful calm serene.
Commanding all to serve her,
From service she is free;
But, ah, my babe so helpless
Is health and wealth to me!


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Gannett's (NYSE:GCI) Q4 and 2012 results, released today, revealed a smaller profit but higher revenue. For the quarter, the company netted a profit of $103 million ($0.44 per diluted share), against $200 million ($0.86) in Q4 2011.
However, revenue grew over that same time frame, by 9% to $1.5 billion. This was the first year-over-year growth in top line since 2006.
Much of the improvement was derived from the company's broadcasting operations, which advanced 44% on a year-over-year basis, in large part because of a record level of spending on political ads during the quarter.
For fiscal 2012, net profit amounted to $424 million ($1.79 diluted EPS), a 7.5% drop from the $459 million ($1.89) of the previous year. Revenues were slightly higher at $5.4 billion.


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The thin ribbon of water that flows
down to Hamburg beneath
locusts and ash trees, but mainly
old birches, where ducks live and coots
that dart off mutely, smelling of the swamps
in the quiet old woods of Stormarn and Holstein,
is called the Alster, and is and always was
a river. It was made into two lakes in the middle
of the great Hanseatic city only when Adolf
III returned to the city from the crusade
in the Holy Land and ordered a miller
to dam the stream with mighty dikes
that would have stopped even the Elbe's
flow. Then a northern sea spread out
amidst the wooden town; all the Holstein waters
of the Wöddelbek, Rönne, Wischbeck and Lankau,
the Sielbek and the Tangstedter Mühlenbach
flowed and could not drain; within weeks
the relentless element, flowing
on and on, grew vaster and
broader, grimly unstoppable,
until first the Outer, then the Inner
Alster (not cut off until much later), became
two lakes, turquoise green today and turquoise
blue tomorrow, and almost always roughened by
the west winds, hemmed by belts of dense reeds and
by now beloved for more than eight hundred years.
So it winds its way, the darkly glittering water-
adder, past bushes and paths, through
the banks' red-branched thickets. And
is utterly silent. A trickling, a softly purling
whisper is faintly heard when it has wood in its
mouth, stones in its bed, polystyrene panels washed
from a construction site somewhere or a spindly
bramble bush that got in its way and that it
drags along for a time as though
the winter-Alster claimed that
none need die as long as
they can play. It is black and half
a man's height higher when it floods.
Over Christmas 2014, after weeks of heavy
rain over the Feldmark and the last scraps of
deciduous woods between Kaltenkirchen, Bad
Oldesloe and Duvenstedt, the Alster's tributaries,
otherwise so idyllic, poured into the river with
unprecedented force and transformed it
within hours into an unpredictable
flood necessitating barricades
of sandbags to protect
the housing estates, and crowds of
rubberneckers were sluiced onto the shifted
shore of the Alster exactly like, as cynics said, flotsam.
Each black meter of water, rolling past ominously
mute, had the momentum of over three
hundred stacked-up fuel tankers,
by someone's calculations.
Meadows, playgrounds, riparian
woodlands, the paths and many streets,
as well as bridges, lots, docks, a big shed at
the foot of the railway embankment holding God
knows what long-forgotten junk were
submerged and sank for days
and for weeks. Children
asked whether the water would
stay like this now, so high, so dark, and
so, so bad. Yes, I said to a little girl
with an eye patch, it looks like
it'll stay like this from
now on. Ah well.
The world is
turning black.
And the neighbor,
arm in arm with his wife,
dog invisible, gazing at a bend in
the Alster where the river used to come
around the curve and fling its gold-brown
glitter at the bank, eyed the nightmarish
immensity of water and said hollowly
that never in his life, since he
sailed boats here as a
schoolboy, had he experienced
the like on the Alster, never had it
happened before, not even in a dream,
in which everything is possible, was it
possible. Too quickly for the darting pupils
to follow, the river rolled under the Fuhlsbütteler
railway bridge southward to the Free and
Hanseatic city. I saw three plastic
canisters and pictured a raft
you could build with them.
High water, said the stunned
neighbor. Floods. They'd always
happened, summer or winter,
in the fall or especially in the spring,
as soon as the snowmelt descended on Stormarn.
But this here, the black water masses, such
a draggled park, never, really, no.
Forced into stone embankments, the Alster
flows past the Rödingsmarkt and the Herrlichkeit
and joins the Elbe between Hamburger Neustadt and
the Portugiesenviertel. Six hours it takes for steamers,
freighters and tankers to reach the sea along the
deep-dredged channel. The three canisters,
a raft that will never be built, since I am
not Tom Sawyer or Huckleberry Finn
and my favorite river is not the Mississippi, but
a stream by which I often linger to gaze at the water
and reflect on the meaning of poetry, these three
pathetic empty plastic containers drift for
weeks from the railway bridge into the
brackish Elbe between St. Pauli,
Finkenwerder and Glückstadt.
Their plastic, cast, molded,
punched and glued in a factory in,
let's say, Hangzhou, before being shipped to
Hamburg along with millions of identical milky white
canisters, requires, unless it's ground to bits,
around 850 years to decompose and
vanish from the earth, as long a span of time
as the two Alster lakes have existed in the middle
of Hamburg. Though no doubt what is true
of the soul is true of plastic. Never,
never does it vanish for good.
In a poem in his collection Rare Earths,
Arne Rautenberg, from Kiel, transforms the
oceans' infestation of plastic into art, into his art,
for which he expresses his thanks (to the tides, the
motion of the waves, and to the ultraviolet light, as well
as to the plankton and the great ocean gyres), his
thanks for being an artist who can play upon
all the continents. Long live art. Long live
the one and only, eternal joy that is
artistic freedom! This means, too,
that everything wants to be art, just as
everything that is at all alive wants to be free.
Plastic, in the year 1800, for Friedrich
von Hardenberg, who called him-
self Novalis, the one who clears
new land, was an aesthetic term,
when he wrote that music, plasticity
and poetry were inseparable elements,
existing together in every free art, just combined
in different proportions. Novalis thought these thoughts
in Burgenland, in Weissenfels on the Saale, which
together with the Mulde, the Müglitz and the
Vereinigte Weisseritz forms a river-land
that he loved, and where he spent
his whole life. All four rivers
flow into the Elbe,
and so, at Barby,
does the Saale, where
Novalis went swimming as a boy,
naked, and often long into the night.
In his whole life Hardenberg never once
held a thing made of plastic. No wonder! There
were no synthetic materials, not even in the hair-
band of a tiny little doll, nowhere in the entire old
world filled with the murmur of the endless
forests, the stillness, the ringing of
bells and the stink of cloacas.
Yogurt cups; lids; clocks; cling wrap;
shopping bags; bags of all colors, sizes and
shapes; toys of all shapes, sizes and colors; cigarette
lighters; disc jackets; pens; car mats; disposable
razors; hub caps; combs; clips; ballpoint pen
cases and cases for cases; bottles; bottle
caps; automobile, tractor, truck and
harvester tires; bowls; plates;
eating utensils; bread bags
and card cases; cases for card
cases; cases for mirror frames; canisters;
disposable chairs; disposable bowls; disposable
tables; plugs; sockets; disposable socket boards; endless
lengths of wires, wires, in sacks and bags, stuffed into
disposable cases; nothing, nothing at all, not the
least little bit of it existed in the as-yet
undestroyed, unwired quiet world,
when Novalis swam in the Saale
without a thought for burning fat,
building muscles or steeling his
chest, but perhaps instead reflecting
whether the bosom is the breast elevated
to a state of mystery, and physics
nothing but the teachings
of the imagination.
For the most part, the plastic
trash which the Elbe sweeps into
the North Sea vanishes there in the sea's
dark abysses. The sediments of the long-since
ravaged sea floor contain inconceivable
quantities of tiny bits of microplastic
particles, mainly fibers, the sum
of which, according to the British Royal
Society's trade journal Open Science, exceeds
by ten thousandfold the larger fragments of plastic
that drift in polluted water gyres, agglomerating
into veritable continents of trash, larger than
Central Europe. If each square kilometer
of the ocean floor were a lake, all
these lakes would be clogged,
one could almost say: shat full of
quadrillions of plastic fibers, all the way
up into the tallest treetops on their shores.
Nowhere on this Earth, at the North Pole,
in the Black, Red or Dead Sea, the
Caribbean or the Antarctic, is there a
large body of water, a coast or a beach with-
out plastic residue, report London scientists
headed by Lucy Woodall from the Natural History
Museum, outside whose façade of noise-insulating
windows the Thames sloshes past, regulated and
polluted. Floating in the world's seven seas,
according to calculations, there are nearly
two hundred and seventy thousand tons
of plastic trash, a horrendous number,
but well-nigh absurd, for it is mysteriously
small compared with the galactic quantities of
plastic trash that all of us actually dump into the sea,
namely an estimated six and a half million tons, for
who can calculate the true weight of the plug
with which we are stopping the world?
Where does it go, all the crap of
affluence, you have to ask. Only
a fraction of the trash, it seems, floats
on the surface in the form of visible particles.
Larger particles break up in the swells, are ground up,
shredded, in part by UV light, into microparticles
that can barely be seen. If algae or microbes
settle on them, they go under, sinking
like ships, airplanes or a corpse
down into the dark
at the bottom.
Lucy Woodall's team analyzed
twelve sediment samples from the ocean
floor collected over the course of twelve years,
up to 2012, in the southwestern Indian Ocean and
in the northeastern Atlantic. Four coral samples, too,
were studied under the microscope and in the
infrared spectrometer. All the sediment
samples contained microplastic
particles, mostly fibers, generally two to
three millimeters long, but often less than tenth
of a millimeter thick. The samples contained
an average of thirteen and a half particles
per fifty milliliters of liquid. More than
half of the particles were viscose,
which is not a plastic, but an
artificial fiber made from
cellulose and used in cigarette
filters, and increasingly in clothing.
Fish, skates, sharks, wales and turtles have
no use for the stuff; for them viscose is poison
from which they will perish, like anyone
who finds nothing left to eat but
plastic. The second most
common material found in all
marine creatures across the globe
was polyester; indeed, one could speak of
polyester fish, polyester water snakes,
polyester octopi. And perhaps,
once the particles are ground smaller
and smaller, until they condense
with the seawater vapors
and rise into the air, one will speak
of clouds of polyester or viscose: plastic clouds.
Due to the small number of samples, it was
impossible to compare the frequency and
composition of the sediments. But fibrous
microparticles seem to be found through-
out the deep sea, ten thousand times
more prevalent in sediments than
in the contaminated ocean gyres.
According to projections, just
one square kilometer of sediment
from the Indian Ocean's deep-sea mountains
contains around four quadrillion plastic fibers. And
studies of the deep-sea valley, the sink for the whole
world's plastic trash, have not yet been undertaken.
Darkest night reigns there. It is lightless and void
of stars. Nothing sparkles. And yet even there
breaths the vast world of the restless stars
that float in the sky's blue ocean.
Photos: Inner and Outer Alster (lakes) in Hamburg (1), a black swan in a blinkered world, the Alster in Hamburg-Klein Borstel (3), the plastic pollution of the oceans and coasts (4, 5, 7, 8), copperplate of Alsleben an der Saale in the 18th century, as Novalis knew the town and river in his childhood (6).
(Translated by Isabel Fargo Cole)


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Brazilian Archives of Biology and Technology
Print version ISSN 1516-8913On-line version ISSN 1678-4324
Braz. arch. biol. technol. vol.48 no.4 Curitiba July 2005
Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion
Leandro Eugênio Cardamoni DinizI; Claudete de Fátima RuasI; Valdemar de Paula CarvalhoI; Fabrício Medeiros TorresI; Eduardo Augusto RuasI; Melissa de Oliveira SantosI; Tumoru SeraII; Paulo Maurício RuasI, *
IDepartamento de Biologia Geral; Universidade Estadual de Londrina; firstname.lastname@example.com; 86051-990; Londrina - PR - Brasil
IIInstituto Agronômico do Paraná; C. P. 481; Londrina -PR - Brasil
The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among_C. arabica genotypes.
Key words: Coffea arabica, genetic variability, RAPD, restriction endonucleases
A variabilidade genética de 40 acessos de cafeeiros de fenótipo arabica foi obtida usando a técnica de RAPD associada a uma digestão prévia do DNA genômico com endonucleases. A variabilidade genética e a relação entre os accessos foram inicialmente avaliadas pela amplificação de 195 primers. Para incrementar a eficiência na detecção de polimorfismo, o DNA genômico de cada acessos foi submetido a digestão com endonucleases antes da PCR. Um total de 24 primers combinados com restrição do DNA gerou 318 bandas, das quais 266 (83,65%) foram polimórficas. A associação entre os 40 acessos foi estimada pelo método de clusters UPGMA, sendo os acessos agrupados de acordo com seu pedigree e aspectos agronômicos. Os resultados mostraram que o uso de enzimas de restrição antes da reação de amplificação pode ser considerada uma ferramenta eficiente para incrementar o número de bandas informativas, possibilitando a diferenciação entre os 40 acessos de C. arabica.
The main varieties of Coffea arabica L. cultivated in Brazil are derived from at least two botanical types; the Bourbon varieties, introduced from the Reunion Islands, and the Typica variety brought to Brazil from the French Guiana in 1727 (Smith, 1985; Berthaud and Charrier, 1988). Currently, coffee is the one of the most important Brazilian commodities. Located in southern Brazil, Paraná state is an important coffee producer. In this state, the Research Center named Instituto Agronômico do Paraná (IAPAR) maintains a Coffea germplasm collection that consist of seven species, several varieties and cultivars, and more than one thousand progenies of C. arabica and C. canephora. Despite its importance, this Coffea collection lacks information about genetic variability, mainly when it is concerning to DNA level. Until recently, genetic diversity among species or cultivars of Coffea arabica was determined using morphological or isozyme markers. However, these markers are unsuitable to measure genetic variation in arabica accessions (Berthou and Trouslot, 1977; Louarn, 1978).
In the last ten years, detection of variation at DNA level has been made possible by the advent of molecular markers. Molecular techniques such as RFLP (Restriction Fragment Length Polymorphism) (Sambrook et al., 1989), RAPD (Random Amplified Polymorphic DNA) (Welsh and McClelland, 1990; Williams et al., 1990) and AFLP (Amplified Fragment Length Polymorphism) (Vos et al., 1995) provide powerful tools for study of genetic diversity. RFLP analysis of the chloroplast genome and the atp-rbc intergenic region were used to study the variation among different taxa of Coffea and two species of Psilanthus. The low sequence divergence suggested that Coffea is a young genus (Lashermes et al., 1996a). The AFLP technique was useful for the detection of introgression in C. arabica (Lashermes et al., 2000). AFLP and SSR markers were used to assess polymorphism between and within coffee accessions (Anthony et al., 2002).
RAPD technique provides a useful tool to identify and estimate genetic diversity in Coffea. Orozco-Castilho et al. (1994) detected polymorphism between 22 C. arabica accessions, one natural interspecific hybrid (Hibrido de Timor), three accessions of C. canephora and one accession of C. liberica. The study also showed that plants originated from Ethiopia and plants from the arabica sub-groups (C. arabica var Typica and C. arabica var Bourbon) were clearly distinguished. Analysis of genetic diversity using RAPD revealed a significant difference between cultivated (Arabica and Bourbon) and wild accessions of C. arabica from Kenia and Ethiopia (Lashermes et al., 1996b). Anthony et al. (2001) studied the genetic diversity among 19 C. arabica accessions from spontaneous and sub spontaneous trees of Ethiopia. The authors concluded that RAPD markers could be applied for DNA fingerprint of coffee accessions, providing information for the introgression of desirable traits and, therefore, increase the effectiveness of the breeding program. Sera et al. (2003) using RAPD markers associated with restriction digestion showed genetic divergence between arabica accessions of the same origin. Chaparro et al. (2004) used RAPD technique for the analysis of 50 wild and semi-wild accessions of C. arabica and concluded that a much larger polymorphism was present in the collection examined than previously reported in others coffee collections. In this study, we assessed the genetic relationship among arabica accessions from a germplasm collection from Brazil using RAPD associated with prior digestion of DNA with restriction enzymes.
Plant material
Forty coffee genotypes were used in this study (Table 1). The accessions were obtained from the Coffea Germplasm Collection of the Instituto Agronômico do Paraná (IAPAR), Londrina, Brazil. Many of the genotypes represented elite cultivars that have been used in breeding programs.
DNA extraction, amplification, and gel electrophoresis
Genomic DNA was isolated from fresh leaves, obtained from at least five different plants of each accession following the CTAB method (Doyle and Doyle, 1987), except that CTAB was replaced by MATAB (Mixed Alyltrimethylammonium Bromide, Sigma) in the extraction buffer. DNA concentration was estimated using a fluorometer (DyNA Quant 200, Höefer-Pharmacia), adjusted to 10ng/µl and bulked by accessions (Michelmore et al., 1991). Amplification reactions were in a volume of 15 µl of a standard PCR and 20ng template DNA. For restriction digestion, genomic DNA was incubated for 1 h at 37º C with one of the following enzymes, Bam HI, Eco RI, or Hae III, just prior to the PCR reaction. DNA amplification was carried out using a PTC 100 (MJ Research) thermal cycler programmed with a 3 min at 94º C for initial DNA denaturation, followed by 48 cycles of 1 min at 94º C, 1 min 45 sec at 38º C, and 2 min at 72º C. The final cycle was followed by a 7 min extension at 72º C. Amplified products were resolved in 1.2 % agarose gel in 1x TAE buffer (40 mM Tris-acetate, 1 mM EDTA pH 8.0) and stained with ethidium bromide. The RAPD profiles were visualized under UV light and stored for further analysis in a PC computer.
Data analysis
DNA markers were scored for the presence (1) and absence (0) of homologous amplified products. The genetic similarity among accessions was estimated using the Dice coefficient of the NTSYS package (Numerical Taxonomy and Multivariate Analysis for personal computer), version 2.1 (Rohlf, 2000). A dendrogram was constructed using the UPGMA (unweighted pair-group method using arithmetic averages) method. The matrix of genetic similarity was also used in a principal coordinate analysis (PCOORD) to resolve the patterns of variation among the genotypes. The cophenetic coefficient between the matrix and the dendrogram was measured using the appropriate routine of the NTSYS package. The bootstrap method was employed to evaluate the reliability of tree topology. The calculations were performed with the BOOD software, version 3.0 (Coelho, 2001). The significance of the cophenetic correlation was estimated with the Mantel correspondence test (Mantel, 1967).
The RAPD technique combined with a prior digestion of genomic DNA with restriction enzymes allowed for the detection of polymorphism among 40 accessions of coffee. From 195 RAPD primers initially screened, only 24 (12.3%) revealed polymorphism. Low percentage of RAPD primers showing informative bands in Coffea were also reported by Lashermes et al. (1996a) and Anthony et al. (2001). The authors attributed the results to the very narrow genetic basis of C. arabica. To increase the number of informative bands, the total DNA from each of the accessions studied were treated with restriction enzymes prior PCR reaction with the 24 primers screened. Only three (Bam HI, Eco RI, and Hae III) out of seven enzymes tested modified the amplification patterns obtained with the primers alone, resulting in 27 primers/enzyme combinations (Table 2). The PCR amplification of genomic DNA from all accessions yielded a total of 318 bands of which 266 (83.6%) were polymorphic. The mean coefficient of variation (CV = 2.6%), estimated using the bootstrap procedure (Coelho, 2001) initiated to stabilize at about 100 markers and the rate of decrease was minimal beyond 200 markers (not shown). These data suggested that 318 markers were adequate for the estimation of genetic relationship among the arabica accessions. DNA polymorphism generated with and without restriction digestion was similar, however, the informative bands obtained per primer increased significantly, often including different amplified products (Table 2, Fig. 1). In wheat genotypes, Koebner (1995) observed that digestion of template DNA prior to PCR amplification allowed for more efficiency of primer annealing along shorter DNA fragment, where a simplified secondary structure was less likely to interfere with the process. Similar results were obtained by Sera et al. (2003) in 14 accessions of arabica coffee and by Silveira et al. (2003) in a study of genetic variability within and among Sarchimor progenies.
Phylogenetic relationship
The dendrogram and the PCO (Principal Coordinate Analysis) estimated from the similarity matrix (Table 3) for the 40 Coffea accessions are shown in Figs. 2 and 3, respectively. The high value of cophenetic correlation (r = 0,81) between the similarity matrix and the dendrogram indicated the extent to which the clustering of genotypes accurately represented the estimates of genetic similarities among the accessions studied.
The primary trend in the dendrogram was to separate the genotypes into two main groups (Figs 2 and 3), one comprising the cultivars of the arabica and the Bourbon type coffee and the other including the cultivars derived from genotypes containing genes of the Hibrido de Timor.
The first cluster showed that C. arabica var typica was highly related to the Bourbon Vermelho cultivar (similarity of 0.94). This result was supported by the origin of Bourbon Vermelho as a spontaneous mutation found in Reunion Island in a field of C. arabica var arabica from Ethiopia (Rothfos, 1980). Both, Arabica typica and Bourbon Vermelho were close to Bourbon amarelo (mean similarity of 0.90) with a bootstrap support of 91% (Figs. 2, 3 and Table 3). High values of genetic similarity between C. arabica typica and the Bourbon group were also reported by Anthony et al. (2001). The authors concluded that even though Bourbon and Typica varieties had accumulated small differences over three centuries of selections, they still presented little divergence with wild material from Ethiopia.
The first cluster also grouped the cultivars Catuaí Vermelho IAC 81, Catuaí Amarelo IAC 17, Catuaí Semperflorens, and Acaiá IAC 474-19. Catuaí Vermelho IAC 81 and Catuaí Amarelo IAC 17 (similarity of 0.92) were artificial hybrids (Caturra Amarelo IAC 476 x Mundo Novo) whose progenies with red and yellow fruits, selected in F3 populations were named Catuaí Vermelho IAC 81 and Catuaí Amarelo IAC 17, respectively (Fazuoli, 1986).
Catuaí Semperflorens was a mutant of Catuaí Amarelo IAC 17, thus justifying the close similarities with Catuaí Amarelo IAC 17 (0.91) and Caturra Vermelho IAC 477 (0.92).
Acaiá IAC 474 -19 was associated with the Catuai cultivars showing a mean similarity of 0.87 with a bootstrap support of 94%. The Acaiá genotype was derived by selection from progenies of the Mundo Novo germplasm, which arose from natural hybridization between Sumatra and Bourbon cultivars (Fazuoli, 1986; Carvalho et al., 1989). Caturra Vermelho IAC 477, Icatu precoce IAC 3282, Icatu Amarelo IAC 2944, Icatu Vermelho IAC 2945, Mokka, and Laurina IAC 870 were grouped together (mean similarity of 0.89). The variety Icatu was obtained after artificial crossing between C. canephora var robusta (4x) and C. arabica var Bourbon Vermelho. The F1 was crossed with Mundo Novo and selected for precocity giving rise to Icatu precoce IAC 3282. The predominance of genes from Bourbon Vermelho in both, Caturra Vermelho IAC 477 and Icatu Precoce IAC 3282 gave support to the high genetic similarities observed. The cultivar Icatu Amarelo was tightly associated in this group showing a mean similarity of 0.90 with Icatu Vermelho, Mokka, and Laurina IAC 870 (Figs 2, 3). Icatu Amarelo was obtained by selection of plants derived from natural crossing between Icatu Vermelho and Bourbon Amarelo (Fazuoli, 1981). Laurina IAC 870 was derived from the Bourbon Vermelho gene pool in Reunion Island, sharing most of its morphological characters with the Mokka cultivar (Rothfos, 1989; Krug et al., 1950). Therefore, the molecular data were fully supported by pedigree information of these cultivars.
The cultivars Rubi-MG 1192 and IAPAR- 77055 were clustered with a similarity 0.82 (Table 3 and Figs. 2, 3). Rubi MG 1192 was a cultivar derived from crossing between Catuai Vermelho IAC 81 and Mundo Novo while IAPAR-77055 was obtained from Icatu x Catuai Vermelho IAC 81 crossing after selection for plant size.
The accessions associated in the second group were clustered into three subgroups (Figs 2, 3). In the first subgroup, the varieties Villa Lobos and San Bernardo were tightly clustered with a similarity of 0.93 and a bootstrap support of. 99%. These varieties associated to Villa Sarchi with a mean similarity coefficient of 0.88 and a bootstrap of 63%. Villa Lobos, San Bernardo, and Villa Sarchi have the same origin (Bourbon Vermelho germplasm) giving support to the high similarities observed. All but one (Colombia Amarelo of the Catimor gene pool) of the accessions associated in the second subgroup belonged to the Sarchimor germplasm (Table 1). IAPAR-59 and the accessions IAPAR 75163-21-10 and IAPAR 75163-12 were obtained from an open pollinated field of IAPAR 75163, which was a F3 progeny from Villa Sarchi (CIFC 971/10) x Híbrido de Timor (CIFC 832/2) (Silveira et al., 2003).
The high similarity coefficients (0.86, on average) obtained with RAPD data were correlated with characteristics such as rust resistance, architecture and vegetative vigor shared by these cultivars. Even so these varieties display some differences in canopy diameter and grain size. A more comprehensive study about the relationships among the Sarchimor varieties and some Bourbon varieties has been described by Sera et al. (2003). The third subgroup associated eight accessions (Semi-erecta, Catuaí Amarelo Erecta, Maragogipe, Purpurascens, Geisha, Cioiccie, Cera, and Goiaba). Semi Erecta clustered with Catuaí Amarelo Erecta with a similarity coefficient of 0.94. The Semi-erecta genotype was a presumed mutant of Bourbon Vermelho sharing characteristics such as plant architecture, productivity, and grain size similar to the Bourbon-type coffee. Although Semi Erecta and Bourbon Vermelho were included in different clusters, the similarity coefficient between them was 0.83 giving support to the morphological relationship. The accession of Catuaí Amarelo Erecta used in this assay was also of uncertain origin. It was found in a field of Catuaí Amarelo IAC 17, possibly derived from hybridization between plants of the Arabica Erecta gene pool and Catuaí genotypes. Catuaí Amarelo Erecta revealed a mean genetic similarity of 81% with Catuaí Vermelho IAC 81 and Catuaí Amarelo IAC 17.
The cultivars Maragogipe, Purpurascens, Cera, Goiaba, Geisha, and Cioicie showed a mean genetic similarity of 0.90. According to Clifford and Willson (1985), Purpurascens, Maragogipe, and Goiaba were mutants of Bourbon Vermelho. Carvalho et al. (1989) considered that Geisha and Cera were mutant versions of Bourbon Vermelho. All these varieties showed a mean genetic similarity of 80% with Bourbon Vermelho. The F2 hybrid Mundo Novo x IAPAR-59 was associated to the second main group with a mean similarity coefficient of 0.83. This hybrid was from a segregant population (Sera et al., 2003) and showed only 0.70 of genetic similarity with Mundo Novo IAC-376 and 0.87 with IAPAR-59. These results were in agreement with morphological characters, suggesting that this segregant carried more genes from IAPAR-59 than from Mundo Novo IAC-376 (Sera et al., 2003).
The remaining cultivars, Superprecoce, Mundo Novo IAC-376, Mundo Novo Semperflorens (a spontaneous mutation of Mundo Novo), and Catuaí SH2 SH3, appeared isolated in the dendrogram. These cultivars were also spread over the PCA plot suggesting that they were less related to the other cultivars. For instance, Mundo Novo IAC-376 and Mundo Novo Semperflorens showed a mean genetic similarity of only 0.75, with the other cultivars studied. Similarly, the cultivar Superprecoce showed a mean genetic similarity coefficient of 0.77 with the other accessions. This cultivar was obtained from accessions of C. arabica collected by FAO in 1968 in Ethiopia. The differences at DNA level reproduce the morphological divergence observed between Superprecoce and the other accessions studied.
The cultivar Catuaí SH2 SH3 was the most divergent genotype among all accessions studied. The range of the similarity coefficient was from 0.66 with Mundo Novo Semperflorens to 0.81 with the cultivar Caturra Amarelo IAC 476. Catuaí SH2 SH3 was a product of crossing between genotypes of Catuaí Amarelo IAC 17 and Catuaí SH2. The F1 hybrid was then crossed to Catuaí SH3, a genotype derived by crossing between Catuaí and the interespecific hybrid C. arabica SH3 (C. liberica x C. arabica), followed by backcrossing with the parental Arabica (Bettencourt, 1981). The grouping of C. arabica genotypes based on molecular markers was consistent with taxonomy and pedigree information described for this coffee collection. The combination of the RAPD technique with the prior digestion of template DNA with restriction endonucleases offered a reliable and effective method of accessing genetic variation in C. arabica accessions.
We thank Conselho Nacional de Pesquisa (CNPq), process number 463861/000, Consórcio Brasileiro de Pesquisa e Desenvolvimento do Café (CBP and D/Café), and Fundação Araucária for financial support. We are also grateful to CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Pesquisa) for the grants to Leandro E. C. Diniz and Valdemar P. Carvalho.
Anthony, F.; Bertrand, B.; Quiros, O.; Wilches, A.; Lashermes, P.; Berthaud, J. and Charrier, A. (2001), Genetic diversity of wild coffee (Coffea arabica L.) using molecular markers. Euphytica, 118, 53-65. [ Links ]
Berthaud, J. and Charrier, A. (1988), Genetic Resources of Coffea. In: Clarke, R. J. and Macrae, R. (Eds.). Coffea Agronomy. Elsevier Applied Science Publishers. pp. 1-42. [ Links ]
Berthou, F. and Troslot, P. (1977), L'analyse du polymorphisme enzymatique dans de genere Coffea: adaptation d'une methode d'electrophorese en serie. Abidjan : Eighth Conference of ASIC. (Ivory Coast). pp. 373-383. [ Links ]
Bettencourt, A. J. and Carvalho, A. (1968), Melhoramento visando a resistência do cafeeiro à ferrugem. Bragantia, 27, 35-68. [ Links ]
Bettencout, A. J. (1981), Transferência de fatores de resistência à Hemileia vastatrix Berk. and Br. para as principais cultivares de Coffea arabica L. Tese (Doutorado), Instituto Superior de Agronomia da Universifdade Técnica de Lisboa, Portugal. [ Links ]
Carvalho, A. and Krug, C. A. (1950), Genética de Coffea XIII -Hereditariedade da característica erecta em Coffea arabica L. Bragantia, 10, 321-328. [ Links ]
Carvalho, A.; Eskes, A. B.; Castilho, Z. J.; Screenivasan, M. S.; Echeverri, J. H.; Fernandez C. E. and Fazuoli, L. C. (1989), Breeding programs. In: Kushalappa, A. C. and Eskes, A. B. (Eds.). Coffee rust: epidemiology, resistance, and management. Boca Raton, Florida : CRC Press Inc. pp. 293-335. [ Links ]
Chaparro, A. P.; Cristancho, M. A.; Cortina, H. A. and Gaitan. A. L. (2004), Genetic variability of Coffea arabica L. accessions from Ethiopia evaluated with RAPDs. Genetic Resources and Crop Evolution, 51, 291-297. [ Links ]
Clifford, M. N. and Wilson, K. C. (1985), Coffee: Botany, biochemistry and production of beans and beverage. The AVI Publishing Company, Inc. [ Links ]
Coelho, A. S. G. (2001), DBOOT - Avaliação dos erros associados a estimativas de distâncias/similaridades genéticas através do procedimento de bootstrap com número variável de marcadores, v. 1.1. Goiânia : Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Goiás. [ Links ]
Cros, J.; Combes, M. C.; Trouslot, P.; Anthony, F.; Hamon, S.; Charrier, A. and Lashermes, P. (1998). Phylogenetic relationships of Coffea species: new evidence based on the chloroplast DNA variation analysis. Mol Phylog. Evol., 9, 109-117. [ Links ]
Doyle, J. J. and Doyle, J. L. (1987), A Rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytoch. Bull, 19, 11-15. [ Links ]
Fazuoli, L. C. (1986), Genética e Melhoramento do cafeeiro. In: Rena, A. B.; Malavolta, E.; Rocha, M. and Yamada, T. (Eds.). Cultura do Cafeeiro. Fatores que afetam a produtividade. Piracicaba : Associação Brasileira para Pesquisa da Potassa e do Fosfato. [ Links ]
Klug, C. A.; Carvalho, A. and Mendes, J. E. T. (1950), Taxonomia de Coffea arabica L. III -Coffea arabica L. var. anormalis. Bragantia, 11, 335-343. [ Links ]
Koebner, R. M. D. (1995), Predigestion of DNA template improves the level of polymorphism of random amplified polymorphic DNAs in wheat. Genetic Analysis. Biomol. Eng., 12, 63-67. [ Links ]
Lashermes, P.; Cros, J.; Combes, M. C.; Trouslot, P.; Anthony, F.; Hamon, S. and Charrier, A. (1996a), Inheritance and restriction fragment length polymorphism of chloroplast DNA in the genus Coffea L., Theor. Appl. Genet., 93, 626-632. [ Links ]
Lashermes, P.; Trouslot, P.; Anthony, F., Combes, M. C. and Charrier, A. (1996b), Genetic diversity for RAPD markers between cultivated and wild accessions of Coffea arabica. Euphytica, 87, 59-64. [ Links ]
Lashermes, P.; Combes, M. C.; Topard, P.; Graziosi, G.; Bertrand, B. and Anthony, F. (2000), Genetic diversity and molecular mapping of coffee. In: Riede, C. R.; Sera T.; Soccol, C. R.; Pandey, A. and Roussos, S. (Coords). In: Seminário Internacional sobre Biotecnologia na Agroindústria Cafeeira, 3., Londrina. Anais... Londrina : IAPAR/IRD. pp. 151-154. [ Links ]
Louarn, J. (1978), Diversité comparée des descendances de Coffea arabica obtennes en autofé condation en fécondation libre an Tonkoui 75-78. In: Charrier, A.ed., Etude de la Structure et de la Variabilité Génétique des Cafëiers. IFCC Bull., 14, 75-78. [ Links ]
Mantel, N. (1967), The detection of disease clustering and a generalized regression approach. Cancer Res., 27, 299-220. [ Links ]
Michelmore, R. W.; Paran, I. and Kesseli, R.V. (1991), Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA, 88, 9828-9832. [ Links ]
Narasimhaswamy, R. L. (1960), Arabica selection S. 795. Its origin and performance: a study. Indian Coffee, 24, 197-204. [ Links ]
Orozco-Castillo, C.; Chalmers, K. J. and Powell, R.W. (1994), Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor. Appl. Genet., 87, 934-940. [ Links ]
Rothfos, B. (1980). Coffee Production. Giordan-Max-Rieck, GmbH, Hamburg. [ Links ]
Rohlf, F. J. (2000), NTSYS-pc Numerical Taxonomy and Multivariate Analysis System version 2.1. Owner manual. [ Links ]
Sambrook, J.; Fritsch, E. F. and Maniatis, T. (1989), Molecular cloning. A Laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 3 v. [ Links ]
Sera, T.; Ruas, P. M.; Ruas, C. F.; Diniz, L. E. C.; Carvalho, V. P.; Rampim, L.; Ruas, E. A. and Silveira, S. R. (2003), Genetic polymorphism among 14 elite cultivars of Coffea arabica L. using RAPD markers associated with restriction digestion. Gen. Mol. Biol., 26, 59-64. [ Links ]
Silveira, S. R.; Ruas, P. M.; Ruas, C. F.; Sera, T.; Carvalho, V. P. and Coelho, A. S. G. (2003), Assessment of genetic variability within and among progenies and cultivars of coffee using RAPD markers. Gen. Mol. Biol., 26, 329-336. [ Links ]
Smith, F. R. (1985), A history of coffee In: Cliford, M. N. and Wilson, K. C. (Eds.). Coffee, Botany, Biochemistry and production of beans and beverage. London and Sydney : Croom Helm. pp. 1-12. [ Links ]
Vos, P.; Hogers, R.; Bleeker, M.; Reijans, M.; Van de Lee, T.; Hornes, M; Frijters, A.; Pot, T.; Peleman, J.; Kuiper, M. and Zabeau, M. (1995), AFLP. A new technique for DNA fingerprinting. Nucl. Ac. Res., 23, 4407-4414. [ Links ]
Welsh, J. and McClelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucl. Ac. Res., 18, 7213-7218. [ Links ]
Williams, J. G. K.; Kubelik, A. R.; Livak, K. J. and Rafalski, J. A. (1990), DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Ac. Res., 18, 6531-6535. [ Links ]
Received: December 22, 2003
Revised: June 28, 2004
Accepted: April 20, 2005


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About the Nikon MC-36
This versatile remote for select Nikon cameras with a 10-pin control port is the perfect addition if you enjoy time-lapse, nighttime, macro and astronomical photography. An onboard timer/intervalometer can be programmed for exposure time and interval time between exposures, giving you an accurate way to compress time while giving your camera the stability it needs with the shutter is open. Requiring only two "AAA" batteries, the backlit display gives you all the information you need to program your picture sequence. A manual trigger release button remains active, even in auto mode, for firing the camera as needed. And it can be locked for long exposures in "bulb" mode.
Product highlights:
- perfect for time-lapse, astronomical, night or macro photography
- programmable timer/intervalometer controls exposure time, frequency and total image limit
- 36-inch cord
- requires two "AAA" batteries
- manual locking trigger release button stays active for firing camera as needed
- warranty: 1 year
- Our 60-day money-back guarantee
What's in the box:
- Multi-function remote trigger release with attached 36" cord (has proprietary 10-pin plug)
- Instruction Manual
- Warranty sheet/proof-of-purchase form
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Overview: The Nikon MC-36 Multi-Function Remote Cord can help prevent camera shake by triggering the shutter release without touching the camera. With the remote, you can manually trigger the shutter release, or program it to automatically trigger the release at timed intervals. The MC-36 is designed for use with Nikon cameras with the 10-pin remote terminal, including D300S, D700, and D800. It has a 36" cable and a 1.25" liquid crystal display (measured diagonally).
Automatic Timer: The MC-36 can be programmed to take pictures automatically at a set interval. Using the remote's LCD, you can set the timer parameters, then press the Timer Start button to begin the program. During the session, the LCD will indicate the number of shots remaining, and the amount of time before the next shot. Shooting will end automatically when the selected number of shots have been taken, or the program is manually stopped. The following parameters can be set:
- Delay: Amount of time before the shutter is released (0 seconds to 99 hours 59 minutes 59 seconds in one-second increments)
- Long: Exposure time (0 seconds to 99 hours 59 minutes 59 seconds in one-second increments)
- Interval: Time between shots (1 second to 99 hours 59 minutes 59 seconds in one-second increments)
- Number of Shots: 1 to 399, or unlimited
- Speaker Status: Audible beeps (On or off)
Manual Release: The MC-36's shutter-release button functions the same way as the shutter release on the camera. Once you have set the exposure, shutter, focus, and shooting modes on the camera, you can take pictures with the remote by pressing the shutter-release button. Pressing the button halfway allows you to check the focus and exposure. For "bulb" shooting, the button can be pressed and slid upward to the Hold position.
Note: Manual operation does not require the presence of batteries in the remote.
Power Requirements: The MC-36 is powered by 2 "AAA" alkaline batteries. The estimated battery life is approximately 2 months, based on continuous shooting with a delay of 5 minutes, an exposure time of 4 minutes 56 seconds, and an interval of 5 minutes.
Compatibility: The Nikon MC-36 Remote Trigger Release is compatible with the following cameras:
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The Clever Coffee Dripper really is a clever brewer, but don't let the name fool you (or double bluff you). Although it may look like a regular dripper, use filter papers, and is even named a dripper, this brew method is actually much more closely related to the cafetiere. It belongs to a group of brew methods, which we call "total emersion" (this just means that we leave all of the brew water mixed with all of the coffee for the duration of the brew time). Most importantly, it means you should be using coffee that's ground for cafetiere brewing, not filter (which you would use for a Hario V60)!
Once you have your coffee ground ready for cafetiere, we can begin our brew…
How to use a Clever Coffee Dripper:
You can brew 1-2 cups of coffee using your Clever Dripper, as it holds up to 500ml of water, plenty for two mugs worth of coffee.
- Start by rinsing your filter paper with water and then popping it into your Clever Dripper. I recommend placing the clever dripper on its coaster for the next steps, in case of any small drips.
- Add 15g of coffee or 1 Hario scoop of ground coffee per cup you will brew. Pro tip – if you want to change things up a bit you can adjusting your "brew ratio" (your perfect ratio of coffee to water) by adding up to 20g of coffee per 250ml of water.
- Pour your hot water (ideally wait 30 seconds after your kettle has finished boiling) into your clever dripper and give it a good stir to make sure all of the grounds are covered/wet and to make sure no grounds have floated to the surface before putting the lid on, this will stabilise the temperature and keep the brew nice and hot!
- Wait for 3 minutes while the coffee brews. If you're brewing in the office, this is the perfect time to walk back to your desk. When the three minutes are up, pop the clever dripper on top of your cup, or if you are brewing 2 cups at once, your jug. This is where the magic happens… when you place the Clever Dripper on top of your mug, the valve will be pressed and the coffee should start dripping into your mug.
- Return your Clever Dripper to the coaster, dropping the filter paper in the bin and giving it a quick rinse. Rinsing straight after brewing ensures you are immediately ready-to-go for your next cup, and better still, this avoids any coffee ground build up which will increase the life of your brewer.
- Enjoy! I'm currently loving Finca El Cairo brewed in Clever Dripper – give it a try!
Will x


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Michigan wildlife group releases photo of cougar taken in the UP
The Associated Press
MARQUETTE, Mich. (AP) — A nonprofit group has released what it describes as perhaps the clearest photograph of a wild cougar taken in Michigan.
The Michigan Wildlife Conservancy said Wednesday the close-up picture was taken June 1 by a trail camera on private property in southern Marquette County. The group declined to identify the landowner.
Michigan Department of Natural Resources wildlife biologist Adam Bump said the agency is familiar with the camera's location and has confirmed the photo is authentic.
The DNR says it has verified the presence of cougars in Michigan at least 15 times in recent years. But the agency says there's no evidence of breeding activity and believes the cats are probably wandering through the state from elsewhere, while the wildlife conservancy believes Michigan has a resident cougar population.
Copyright 2012 The Associated Press.


text:
The state Supreme Court without comment Wednesday upheld the ongoing transfer of water from Imperial Valley farmers to San Diego County.
But in doing so the court untied only one of many legal knots involving the water sale and broader 2003 seven-state pact to share the Colorado River.
"Certainly we are relieved and very pleased with the result," said Dan Hentschke, general counsel for the San Diego County Water Authority.
The case now returns to Sacramento County Superior Court to hear some thorny environmentally-related challenges associated with the transfer and river deal.
The Imperial County Board of Supervisors and the county's Air Pollution Control District were two of a number of parties challenging the transfer.
"We are looking forward with great enthusiasm in trying the environmental case in Sacramento," said Antonio Rossman, an attorney representing Imperial County. "We emphasize, as did the court of appeal, that the (agreement) has not been validated environmentally,"
Much is at stake for the San Diego region. In 2011 alone, the Imperial Irrigation District sold San Diego 80,000 acre feet — enough to serve the needs of 160,000 average households for the year. Eventually the amount ramps up to 200,000 acre feet annually.
Steve Bilson, a founding member of Protect Our water and Environmental Rights, which has been challenging the agreement, said water agencies are using ratepayer dollars to protect the transfer and broader Colorado River deal "to keep the taxpayers on the hook. It's the little guy who is stuck with rising water rates year after year and no recourse."
Imperial County and its air pollution district contend that elements of the transfer will speed the decline of the troubled Salton Sea, straddling Imperial and Riverside Counties. As the lake recedes, the exposed playa could be whipped up by frequent wind storms, posing health risks to residents.


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THE ULTIMATE 11: SNK FOOTBALL CHAMPIONSHIP is a soccer game released by SNK in 1996. Choose your favorite of 80 different teams, more than ever before, and conquer the two Championship modes! Choose from 4 styles of team balance to customize your team according to your own playing style! The "ACA NEOGEO" series has faithfully reproduced many classic NEOGEO masterpieces. Players can change various game settings such as game difficulty, and also reproduce the atmosphere of arcade display settings at that time. Players can also compete against each other from all over the world with their high scores. Please enjoy the masterpiece that built a generation for video games. *This title is based off the MVS (NEOGEO for arcades) version. There may be differences between this version and the versions for the home NEOGEO console and other home consoles.
Additional information
Published byHAMSTER Corporation
Copyright©SNK CORPORATION ALL RIGHTS RESERVED.
Developed byHAMSTER Corporation
Release date2/28/2019
Approximate size227.15 MB
Age ratingFor all ages
InstallationInstall on your home Xbox One console plus have access when you're connected to your Microsoft account.
This product needs to be installed on your internal hard drive.
Additional termsXbox Live code of conduct
ACA NEOGEO THE ULTIMATE 11: SNK FOOTBALL CHAMPIONSHIP privacy policy
Terms of transaction
ACA NEOGEO THE ULTIMATE 11: SNK FOOTBALL CHAMPIONSHIP license terms
Arcade Archives Series Produced by HAMSTER Co.


text: djheadhunterz Headhunterz Utrecht Vote for Headhunterz at the Hard Dance Awards! The annual Hard Dance Awards has once again opened its portal to receive your votes. It's already the 8th time the Hard Dance Awards are being held and it's bigger than ever before. The doors are wide open, so don't hesitate to place your vote and show your support for Headhunterz. The yearly voting poll is waiting for your personal vote! 2010 has been a crazy year with many great moments for Headhunterz. It started off with a sold out X-Qlusive at the Heineken Music Hall: Studio Sessions (CD): Studio Sessions 'World Tour': A 'Beatport Music Award': Voted in the Dj Mag Top 100 as the number #1 Hard Dance Dj and several epic anthems. This producer has shown you what Hard Dance is all about. If you feel and think that Headhunterz is your personal favourite; please vote for him at the Hard Dance Awards. It is your turn to tell the scene what and who has been smashing out there in the Hard Dance scene. Let your cursor lead to www.harddanceawards.com and start voting in the different categories. In 2010 the Hard Dance Scene evolved even more than ever before. The scene is growing massively and the passion for Hard Dance music is spreading around the globe. Here are the categories for 8th Annual Hard Dance Awards where Headhunterz is involved: · Best European Hard DJ · Best International DJ · Best European Hard Producer · Best International Hard Producer · Best Track · Best Artist Album · Best Label If you don't have any inspiration we've summerised a few high lights of Headhunterz in 2010. X-Qlusive Headhunterz (Sold out 6 weeks before the event) Headhunterz and Endymion won a Beatport award for 'Save Your Scrap for Victory (RMX)' Headhunterz voted in the Dj Mag Top 100 as the number one Hard Dance Dj Headhunterz - Psychedelic Brooklyn Bounce – Club Bizarre (Headhunterz & Noisecontrollers RMX) Headhunterz – Studio Sessions (CD) Headhunterz – Studio Sessions (World Tour) Headhunterz created the official anthem of Defqon.1 AU and had the honour to close the mainstage Thank you for your support and votes.


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ASTORIA — Crescent Lounge, which had been in the neighborhood for nearly 13 years, had its last night of business this week.
The bar, which opened in December 2001 at 32-05 Crescent St., shuttered Tuesday night. Owner John Karanikas said he decided to put the watering hole up for sale last year to move on to his "next chapter."
"It's been a great run," said Karanikas. He said the small and cozy bar, where he started as a bartender, made for a friendly atmosphere that was home to many close and loyal regulars.
"It was a very close-knit, very good relationship with our customers," he said. "A lot of people say, 'It's family,' but it really has been."
Karanikas said customers have known the bar was on the market, but the actual closing date came sooner than expected. He said he's not sure what the next owners plans are for the space.
News of the Crescent Lounge's closure prompted several comments on the bar's Facebook and Yelp pages, with customers sharing memories of their time there.
"The Crescent Lounge was what finally made me feel like New York was home," one person wrote on Facebook.
"There's so much love that we've had from our customers," Karanikas said.
"I'd like to thank Astoria," he added. "It's a wonderful place to have a business."
In other local restaurant news:
►Pizzeria L'Inizio opened Thursday at 47-23 Vernon Blvd., serving up Neapolitan-style pizza by the slice, which is cooked in a brick oven and made with dough that's prepared two or three days in advance to ferment.
"It makes the dough really nice and airy and fluffy, and it brings a beautiful texture and a taste to it," said owner Tom Blaze.
Right now, the space is casual, with counter service and pizza available by the slice or by the pie. A small garden in the backyard supplements the menu's ingredients with things like fresh basil and tomatoes, Blaze said.
Over the next few months, he plans to open a cheese and charcuterie bar in the back of the eatery, which will feature homemade cheeses and a number of cured meats.
The menu will eventually include paninis, salads and appetizers, Blaze said. He hopes to serve beer and wine in the future as well.
►L'Arte del Gelato is now operating a gelato cart outside the Falchi Building's Food Box market at 31-00 47th Ave., which will be open on weekdays from 11 a.m. to 6 p.m. through the fall, according to a rep for Jamestown, which owns the building.
The cart will serve 12 flavors of gelato, with classics like chocolate, hazelnut and pistachio as well as flavors that will rotate weekly, including seasonal options like strawberry and fig.
L'Arte del Gelato is set to occupy a 6,000-square-foot space on the ground floor of the Falchi Building in October, which will be used as a production site to make gelato for its retail locations, including its main store in Chelsea Market.


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Sore breasts with bloating and gas
February 28th, 2010
I am on Implanon, i have been having really sore breasts for about 2 weeks. went up a size in breasts. i have been feeling funny, just not my self. i do not have periods due to the birth control. so i dont know if i missed it...i have been having pains like cramps about 2 weeks ago for like 2 days. then they went away, now they are back with bloating and gas.ive had some spotting today and yesterday and during the last week my discharge has been like slimy and not usual. i have been feeling constipated. i took a test tonight at 9pm. it had a little faint line there on the positive side. ive felt really warm lately. i dont know why. i feel a lot more worn out when i walk up my staircase. i called my ob-gyn and they said that its impossible with implanon.
i am normally from WA state but visiting husbands parents, in Colorado, husband thinks ive been feeling all these because of the elevation difference as well as the cold.
please help me!
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oh gosh, exactly the same thing is happening to me right now. did you end up finding out what was going on?
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Community Volunteer
It is unlikely, but not impossible, to get pregnant while on implanion. If you popped a positive (no matter how faint the line was) on a pregnancy test, you need to see your doctor, who can confirm a pregnancy with a blood test.
Implanion can cause changes in your cycle, sore breasts, and weight gain, among other things. It is best to seek the advice of a medical professional if you are having questions or problems with your birth control.
My only other advice to you is to maybe find another doctor...one who knows it is UNLIKELY but not IMPOSSIBLE to get pregnant on hormonal birth control.
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Bali Textiles 109
Bali Textiles 109 Bali Kain cepuk superb w green tones and vibrant pattern exc condit, full size: 260cm x 86cm (IK) A Balinese weft ikat silk and gold thread sarong, late 19th- early 20thC. (IK) a Bali weft ikat silk selandang with gold thread work, late 19th- early 20thC. (IK)[…]Read More »


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The opposite of a democracy is an autocracy. A democracy is a government chosen by its citizens. An individual without the input of the country's citizens governs an autocracy. Autocratic rulers make economic, social and political decisions without consent from the citizens.
An autocratic government has unlimited power over its country. Terms such as "tyrant," "despot" and "dictator" are often heard when referring to an autocratic government. Several monarchies, with power given to the monarchs based on heredity, ruled based on autocratic principles until their countries formed constitutions, resulting in diminished autocratic power.
Countries such as Russia, China and Germany have had autocratic rulers.