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TUSC2 is a highly conserved lung cancer candidate gene. Zusätzlich bieten wir Ihnen Tumor Suppressor Candidate 2 Proteine (4) und Tumor Suppressor Candidate 2 Kits (1) und viele weitere Produktgruppen zu diesem Protein an.
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Human Polyclonal TUSC2 Primary Antibody für ELISA - ABIN451824
Deng, Kawashima, Wu, Jayachandran, Xu, Minna, Roth, Ji et al.: Synergistic tumor suppression by coexpression of FUS1 and p53 is associated with down-regulation of murine double minute-2 and activation of the apoptotic protease-activating factor 1-dependent ... in Cancer research 2007
Low TUSC2 expression is associated with bladder cancer.
TUSC2P can suppresses the tumor function of esophageal squamous cell carcinoma by regulating TUSC2 expression and may also serve as a prognostic factor for esophageal squamous cell carcinoma patients.
these findings show that the combination of TUSC2-erlotinib induces additional novel vulnerabilities that can be targeted with Auranofin.
Data show that TUSC2 is a direct target of miR-584, which is transcriptionally regulated by TWIST1.
FOXP1, TP53INP1, TNFAIP3, and TUSC2 were identified as miR-19a targets.
The therapeutic activity of TUSC2 could extend the use of erlotinib to lung cancer patients with wildtype EGFR.
TUSC2P and TUSC2 3'-UTR expression inhibits cell proliferation, survival, migration, invasion and colony formation, and increases tumor cell death. TUSC2P and TUSC2 3'-UTR bind to miRNAs and arrest their functions, leading to increased TUSC2 translation.
The tumor suppressor gene TUSC2 (FUS1) sensitizes NSCLC to the AKT inhibitor MK2206 in LKB1-dependent manner.
RNA sequence elements in FUS1 UTRs that regulate FUS1 protein express were identified.
Absence of tumor suppressor FUS1 protein expression is associated with bone and soft tissue sarcomas.
Data show that the recombinant FUS1 plasmid has been successfully cloned, which allows highly efficient FUS1 expression in E.coli strain Rosetta (DE3)2 plys.
The goal of this study was to analyze possible involvement of TUSC2 in malignant pleural mesothelioma.
Myristoylation is required for Fus1-mediated tumor-suppressing activity and suggest a novel mechanism for the inactivation of tumor suppressors in lung cancer.
These results suggest that miR-378 enhances cell survival, tumor growth, and angiogenesis through repression of the expression of two tumor suppressors, Sufu and Fus-1.
FUS1 gene and Fus1 protein abnormalities could be used to develop new strategies for molecular cancer therapy for a significant subset of lung tumors.
These results suggest that the three miRNAs are negative regulators of Fus1 expression in lung cancers.
Overexpression of candidate tumor suppressor geneFUS1 isolated from the 3p21.3 homozygous deletionregion leads to G1 arrest and growth inhibition oflung cancer cells.
results suggest that Tusc2 acts as a positive regulator of RANKL-mediated osteoclast differentiation
Fus1 achieves its protective role in inflammation, autoimmunity, and cancer via the regulation of mitochondrial calcium and calcium-coupled parameters.
This gene is a highly conserved lung cancer candidate gene. No other information about this gene is currently available.
PDGFA associated protein 2
, PDGFA-associated protein 2
, fus-1 protein
, fusion 1 protein
, tumor suppressor candidate 2
, lung cancer candidiate, FUS


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Websites are your electronic brochure and the gate way for potential clients to gain information about who and what your company is all about. Websites should be informative, clean and easy to navigate. Potential clients are not necessarily looking for flash and fancy graphics. They are looking for information that may help them make an informed decision about the product or service you may offer. The easier the website is to read and understand, the more likely you will be able to capture the client. If a potential client has to wait for downloads and navigate through multiple pages to find what they are looking for, they will leave your site frustrated and find the information else where. Rock The Mike Productions can build your website for you or we can show you how to build your own and maintain it properly. Knowing how to manage your website is a valuable tool that allows you to respond quickly to your potential clients and get them the service they are looking for.
Here are a few live sites that are currently being used by Rock The Mike Productions Clients.
AutoXloo.com Automotive Marketing Platforms
866DrKaren.com Chiropractic Care
USAInsuranceClaims.com Lawyer & Public Adjuster Service
CleanBloodStream.com UVL Blood Labs, Inc
To learn more about building the right website for your company, Please Call Mike Harter at 727.678.5064.

subset: commoncrawl


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YouTube to MP3 Converter and Downloader
I found some cool music and searched over YouTube. I got some related music videos. I wanted to download these as mp3 files to listen these as audio. The tracks were really old/classic and I kind of did not want the video part of those but the tracks were really awesome. Now all I have to search for a YouTube to mp3 converter. I found a great one free YouTube to mp3 converter.
Free YouTube to MP3 Converter. Extract and download audio from YouTube video and save it as MP3 file on your PC with one mouse click and with this YouTube Converter! Free YouTube to MP3 Converter contains no spyware or adware. It's clearly free and absolutely safe to install and run. The interface is very simple and self explaining. There is a field for YouTube URLs, a field for an output path, and a list with preconfigured profiles. You can adjust mp3 quality for the target to be downloaded.
How to use
- Use your Internet browser to select and copy YouTube link you want to download. Click the Paste button and wait a few seconds while the program is detecting YouTube video.
- If you download many videos at one time it can be useful to save the list of these YouTube links. Just make the right click of the mouse and select the Save list… item or press Ctrl+S.
- Click the Browse… button and choose the location where you would like to save your downloaded audio files. Click Ok.
- By default the output file name is the same as YouTube video title is. If you want to change it click the Output Name… button. The new window will be opened.
- Click the Tags… button to set tags for output MP3 files. In the MP3 Tags window specify the track number, title, artist and other parameters as you want.
- In the list of Presets select one of the available pre-configured MP3 presets.
- Click the Download button to downlolad your desired target as mp3 file.
Download YouTube to MP3 Converter
Topic: Downloads, Windows Softwares, Converters, YouTube, Social Streams, Audio & Video
Tags: Audio, converter, Downloader, youtube, music, mp3, youtube to mp3 chrome, change youtube to mp3, youtube mp3 converter chrome
Hiroshi is a senior web developer, entrepreneur, founder and CEO of TechMynd. You can subscribe to TechMynd's newsletter to receive free software, giveaways, technology news and updates in email. Become our fan on Facebook, follow us on Twitter. Got any question? Contact us or visit us on our Google Profile.


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ForumsQuestionsHiding future tasks on the iPad app and new BETA mobile website
Hiding future tasks on the iPad app and new BETA mobile website
Alister Cruickshanks
Posted: Feb 06, 2012
I have many tasks that I setup to reoccur at various intervals. Once I complete these tasks they then reoccur as a future task. Some are weekly, monthly or even yearly.
On the main website you can change th default setting for future tasks to hide unless it Is on the actual day (0 days showing of future tasks). But on the iPhone and iPad app and also on the new BETA mobile website there doesn't appear to be a way of changing th default of future tasks.
The consequence is I have a huge list of over 30 tasks that I don't want to be displayed that are due shortly. I want these tasks to only show up on the day they are due.
Does anyone know of a way to do this as It is very frustrating on the iPad app!
Many thanks
There's a Help topic for that. ;)
How do I filter and sort tasks on iOS?.[/quote]
Alister Cruickshanks
Posted: Feb 06, 2012
The filter doesn't work for tasks with a start date less than 30 days sadly. That's the problem, even enabled they all still show up.
Which filter doesn't work?
The "Hide Future Tasks" filter will hide tasks that have a Start Date that is more than 1 day in the future.
Alister Cruickshanks
Posted: Feb 07, 2012
The "Hide Future Tasks" Filter on the Ipad/Iphone App still shows all the tasks up to 30 days, so it doesn't work.
Do you think this is because of a bug since I set Due Dates as well as Start dates on the same task as the same day?
Alister Cruickshanks
Posted: Feb 07, 2012
I just tested that theory. I left the Start Date on my tasks and removed all the Due Dates for the same tasks.
Went back into the App and refreshed and they are still all showing up inspite of the filter "hide future tasks" being enabled.
Toodledo Founder
Can you please provide an example of a start-date/due-date combination that is not being hidden when the "hide future tasks" filter is in place?
Alister Cruickshanks
Posted: Feb 07, 2012
Yes, I have many but for example, I have a task;
"Check currency prices" - start date 9th feb 2012 - due date 9th feb 2012
The filter is set to hide future tasks but it still shows up. I have tasks due for tomorrow, next week and two weeks time and all of them show up.
I have been playing around and decided to try and remove the due dates and to be honest it doesn't make any difference, so it appears as though it is not connected to the combination.
After Changing all my tasks to have no due dates but only start dates in the future, then all still show up.
I View my list always by folder with the filter always enabled
Alister Cruickshanks
Posted: Feb 07, 2012
Been playing around again, and it appear tasks ONLY over 30 days are hidden when the hide future tasks is enabled . Tasks less than 30 days is shown.
When the hide future tasks filter is turned to "show all" then all tasks including those over 30 .days are also shown
Toodledo Founder
I was not able to reproduce this error. I created a new task with a start date and due date of Feb 9th as you suggested. I then applied the "future tasks" filter on both m.toodledo.com and our iOS app and the task was hidden in both places as expected. Can you please open a support ticket and include some screenshots that show how you are applying the filter and the task that is showing up that shouldn't.
Alister Cruickshanks
Posted: Feb 08, 2012
I will raise a support ticket shortly.
I probably will need to take a photo of the screen as I don't know how to do a screen shot on the Ipad!
Many thanks for your help.
Toodledo Founder
Screenshot on the iPad is done by holding down the lock button and pressing the home button. You can Google "iPad screenshot" for more details.
Alister Cruickshanks
Posted: Feb 08, 2012
Thank you....however Whilst taking Photos I noticed something.
I noticed that I could not see the "Start Date" listed on the task like on the main website.
Then, I went it Settings, and saw that Start Date was listed as an unused field. (under fields and defaults).
So....I moved it to add the field, closed and then reopened the App. and Hurrahh, it works!
So....it seems that the "Hide Future Tasks filter" ONLY Works if you have the "Start Date" added as a field via the settings.
Anyway, Thank you for your help. If you hadn't have asked me to take some photos, I would never had found the answer!
Alister Cruickshanks
Posted: Feb 08, 2012
... The confusion was I think that I tend to use the Website to create tasks, but use the Ipad App to tick them off and check what's coming up!....So I never noticed that Start Date was missing on the App!
Toodledo Founder
If the start-date field is not used, then the start date will not be examined when applying the filters.
Glad you figured it out.
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Plumbing Work
Emergency and general plumbing, leaking taps, backed up toilets and blocked sinks and more.
Heating & Boiler Repairs
Fast and reliable services for the Leeds area. Gas boiler repairs, central heating, leaks and safety checks.
Blocked Drains & Toilets
Sinks, toilets, drains and manholes unblocked using rods or high pressure drain jetting equipment.
Unvented Hot Water
We are fully qualified to repair and service all types of unvented hot water systems and cylinders.


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Cow Parsley Flowers Rubber Stamp
- Availability: In Stock
- Brands The Old Island Stamp Company
- Product Code: 922-D
Umbellifers are plants with showy clusters of flowers, and the cow parsley is one of the best known. This stamp is so impressive when embossed, stamped alone or with other flora it will truly make a lovely card for someone.
2 1/4" high by 2 3/4" wide
Dimensions given are for block size, actual image will be 1/4" less


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Noodle's Summer Camps is in beta. The information you find may be out of date or incorrect. Sign up to be notified when we launch Summer Camps (we won't email you for anything else!).
Interested in having your summer camps or information listed on Noodle? Contact us at firstname.lastname@example.com.
Main Search Results
Discover and research the 1 Children summer camp in Hopkins, MN with Noodle. Narrow Hopkins, MN summer camps by age group and camp offering to find the right camp for your family.
110 Blake Rd S, Hopkins, MN 55343Outdoor Activities


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Doping in sport continues to be a serious issue at all levels. However, it is possible for everyone to contribute to the mission of WADA "to promote and co-ordinate at an international level the fight against doping in sport in all forms". In March 2003, the IPC signed the World Anti-Doping Code. Thereafter, the IPC Anti-Doping Code was revised to comply with the World Anti-Doping Code and WADA´s standards, and was officially launched today. This Code will apply at all IPC sanctioned competitions including the ATHENS 2004 Paralympics.
Said IPC President, Phil CRAVEN: "In preparation for the ATHENS 2004 Paralympics and to protect the athletes´ fundamental right to participate in doping free sport, the IPC has ruled that all National Paralympic Committees and sports wishing to take part in the Paralympics must declare their acceptance and recognition of the World Anti-Doping Code. Those that do not, will be deemed ineligible to participate. We believe that this is the strongest message we can send to our members. Supporting WADA in their work, the IPC will not tolerate doping in sport."
The WADA Athlete Outreach Program is present at the IPC Alpine Skiing World Champs and will be at the ATHENS 2004 Paralympics from 17 to 28 September. The program strives to build greater awareness about WADA and the anti-doping issue and to give athletes an opportunity to be involved, active and informed about anti-doping issues. The WADA Independent Observer team will also be present during the Paralympics to act as the "eyes and ears" of the outside world by observing and reporting on all aspects of the doping control operations of the competition in a neutral and unbiased manner. These are only a few of the initiatives that the IPC and WADA are working on together. On 26th January 2004 the IPC and WADA signed an amended version of the 2001 memorandum of understanding, agreeing to further joint actions and commitments.
"We are very pleased to have an awareness program at these championships," said Richard W. POUND, WADA president. "By inviting WADA to have such a presence and by implementing the Code, the IPC is once again demonstrating its strong commitment to ridding sports of doping."
World Sailing Newsletter
World Sailing Newsletter is the weekly online newsletter of World Sailing.
It features the latest news and events from the sailing world together with features and info in an easy-to-use format.
It features the latest news and events from the sailing world together with features and info in an easy-to-use format.


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How are you working for healthy food for all and thriving communities in New England?
I grew up on an organic farm in mid-coast Maine. In the mid-90's, as family farms in my community were shutting down, including our own, I realized how important it was for society to recognize the benefits small-scale farming had on the community, the environment, and the economy. I studied agriculture as an undergraduate and traveled globally to compare industrial and sustainable farming practices. I obtained my Masters in Public Health with a focus on child malnutrition and sustainable agriculture solutions in Haiti.
After college, I went back to my roots and began working at the Maine Organic Farmers and Gardeners Association (MOFGA) under Russell Libby's leadership. Since MOFGA, I've taught nutrition education to low-income Mainers, served on community food councils, farm to school work-groups, and have been selected as a Maine delegate to the New England Food Summit in 2014 and 2015. I currently work as an agriculture and food policy staffer for Congresswoman Chellie Pingree of Maine's first district.
What successes and challenges do you see in your work to achieving the Vision of "50 by 60" with racial equity and social justice, healthy food for all, sustainable farming and fishing, and thriving communities?
One of the successes - and challenges - is that we are all in this together and come to the table with our own perspectives. Whether you're a farmer, fishermen, legislator, or work for a nonprofit, everyone's voice brings valuable perspective to building a sustainable food system. Sometimes the conversations get messy or sidetracked, but the end result is a collaborative effort reflecting all communities and their needs. Regionally I think we are on a great path.
Are you using the Vision in your work?
Absolutely. The New England Food Vision is a tangible document that helps me tell the story of regional partnerships in New England and Maine. And this is especially important when working with groups outside of these efforts.
Emily Horton at the launch of the first ever mobile farmers market in Maine, the Good Food Bus.


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Our Commitment to You
At Calcagno Dentistry, we want to make your appointments as easy and comfortable as possible. From our helpful office staff to our professional dental assistants and hygienists, our commitment to serving you goes beyond clinical care. We strive to get to know each of our patients personally and to earn their trust. The little details are important to us, so we offer TVs and warming blankets in our exam rooms, and can help more anxious patients relax with nitrous oxide. We are always exploring new options to help our patients feel as relaxed and comfortable as possible, so let us know if there is anything we can do to make your appointment better.
Making Informed Choices About Your Dental Care
Dr. Calcagno and our dedicated team are committed to providing you with the necessary resources to help you make good decisions about your care. Explore our website to learn more about what to expect during your appointment, our staff, and how we have helped other patients just like you.
Come Visit Our Office!
If you'd like to come visit our office, then schedule a consultation by calling Calcagno Cosmetic and Family Dentistry today at (507) 281-3659. Conveniently located in Rochester, MN, our office also welcomes patients who live in Red Wing, St. Charles, Winona, Farmington, Austin, and all surrounding communities.


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Złoty Krąg | Z wizytą przy Kerid i na farmie Fridheimar
Wybierz się na wycieczkę minibusem po Islandii, podczas której będziesz podróżować po słynnej trasie Złotego Kręgu, w komfortowy sposób. Odwiedzisz Park Narodowy Thingvellir, obszar geotermalny Geysir i wodospad Gullfoss oraz zatrzymasz się również przy znanym w kraju kraterze Kerið. Dodatkowo czekają Cię odwiedziny na farmie, gdzie hoduje się islandzkie konie. To idealne rozwiązanie jeżeli szukasz okazji do pełnego eksplorowania słynnego obszaru nazywanego Golden Circle.
Odbierzemy Cię o poranku, a następnie pojedziesz w stronę krateru z jeziorem na dnie - Kerið, który powstał podczas erupcji ponad 6000 lat temu. Możesz chodzić po jego krawędziach, aby podziwiać krajobraz lub zejść na brzeg jeziora, aby z bliska zobaczyć turkusową wodę oraz żywe kolory pokrywające zbocza krateru.
Dalej jest farma Friðheimar, gdzie zobaczysz islandzkie konie. Te zwierzęta słyną z inteligencji, niewielkich rozmiarów, przyjaznego zachowania i pięciu chodów. Spodziewaj się specjalnie przygotowanego pokazu, podczas którego na własne oczy przekonasz się o zdolnościach tej rasy. Lunch (na własny koszt) zostanie zorganizowany w szklarni, gdzie po wizycie w stajniach będzie można kupić lokalne produkty.
Następnie wsiądziesz do minibusa i pojedziesz w stronę wodospadu Gullfoss (nazywanego "Złotym"), który jest uważany za jeden z najpiękniejszych w kraju. Możesz go podziwiać korzystając z dwóch platform widokowych i fotografować potężną kaskadę spadającą z dwóch skalnych półek prosto do starego, głębokiego kanionu.
Dalej znajduje się obszar geotermalny Geysir, wraz ze swoimi słynnymi gorącymi źródłami. Jednym z nich jest gejzer Strokkur, który co kilka minut wyrzuca w powietrze potężny słup wody.
Ostatnim, ale nie mniej ważnym punktem wycieczki, jest Park Narodowy Thingvellir, który posiada znaczenie historyczne oraz geologiczne. To tu powstał w 930 roku islandzki, demokratyczny parlament, a dodatkowo dolina leży na styku dwóch płyt kontynentalnych!
Zobacz najsłynniejsze atrakcje na Islandii i spędź czas podróżując w wygodnym minibusie. Sprawdź dostępność tej wycieczki po Złotym Kręgu, wybierając datę.
Kilka faktów
- Dostępna: Cały rok
- Długość pobytu: 8 godzin (y)
- Aktywności: Zwiedzanie, Kultura
- Poziom trudności: Łatwy
- Minimalny wiek: 8 lat
- Języki: English
- Obszar: Złoty Krąg,
The Golden Circle is a 300 kilometre (186 mile) route to the three most popular natural attractions in Iceland: the Geysir Geothermal Area, Gullfoss Waterfall and Þingvellir National Park.
This sightseeing trail is easy to do within half a day from Reykjavík, either by self-drive or on one of many tours. Some of these tours have additional activities, such as snorkelling or snowmobiling, or are conducted in a unique style, such as by helicopter, or under the midnight sun.
Within the three locations of the Golden Circle are some of the clearest examples of Iceland's fascinating geological forces, magnificent landscapes, and rich culture.
The Geysir Geothermal Area
The Geysir Geothermal Area is a hot-spring haven in Haukadalur Valley. As could be guessed, it is home to a famous geyser, the name of which has named all others: Geysir itself.
Though this feature is currently in a phase of inactivity, its neighbour Strokkur more than makes up for it. Every five to ten minutes, it blasts a column of boiling water to heights that can reach over 40 metres (131 feet).
The surrounding area is dotted with fumaroles, hot springs, and mud-pits. There are also two other smaller geysers, Smiður and Litlí-Strokkur, that can be easily visited, as well as a hotel, restaurant, cafe and gift shop across the road.
Gullfoss Waterfall
The 'Golden Waterfall', Gullfoss one of the most beautiful and powerful waterfalls in Iceland, plummeting 32 metres (105 feet) in two tiers into the river gorge of the popular rafting river Hvítá. It is just a ten-minute drive from Geysir and is the furthest point on the Golden Circle from Reykjavík.
Gullfoss was very almost lost in the early 20th century when British developers sought to harness its incredible power for geothermal energy. Though they got the lease to the land, allowing them to go ahead with their plans, they met an unlikely adversary: the daughter of the farmer who owned it, Sigríður Tómasdóttir.
This resilient woman refused to see the waterfall, to which she and her sisters paved the first path, destroyed. She, therefore, walked over 200 kilometres (124 miles) to Reykjavík and back multiple times to meet with a lawyer in order to help change the decision.
Though the process was arduous and took years, eventually Sigríður managed to exhaust the resources of the businessmen and they withdrew their plans. Because of her, all Icelandic waterfalls are now protected from foreign investors, and she is considered one of Iceland's first environmentalists and most important historical people.
Thingvellir National Park
The largest attraction of the Golden Circle is Þingvellir National Park. The Icelandic parliament was founded here in 930 and remained until the year 1798 before moving to Reykjavík, making it the original site of what is now the world's longest ongoing parliament.
Today it is a UNESCO World Heritage Site and one of the most important places to visit in Iceland. This is not just for its historical and cultural values, but for also its magnificent landscape and dramatic geology.
Þingvellir is surrounded by a beautiful mountain and volcanic ranges, as it is located in a rift valley directly between the North American and Eurasian tectonic plates. This makes it one of the few places in the world where you can walk between the continents, in the Mid-Atlantic Rift.
The daring and qualified even have opportunities to snorkel or scuba dive in this no-mans-land, in a ravine filled with crystal-clear spring water called Silfra. The visibility here can exceed 100 metres (328 feet), revealing incredible geological sites. Though it never freezes over due to the constant flow of water into it, it is 2°C (35°C) throughout the year, thus underwater explorers are always equipped with protective suits.
Other sites of note are the magnificent Almannagjá gorge, which you can walk down into to reach the rift valley from the North American tectonic plate, and the beautiful lake Þingvallavatn, the largest lake in Iceland, which gleams to the south of the National Park.
Surrounding sites
Due to its convenient location in south-west Iceland, it is easy to visit the Golden Circle alongside with many other sites. Those seeking relaxation could head to the Secret Lagoon in Flúðir or Fontana Spa in Laugarvatn, those seeking adventure could book a trip to the ice tunnels of Langjökull glacier, and those seeking culture could visit the Sólheimar ecovillage. For more natural beauty, you can visit the nearby Kerið crater lake.
There are many tours that include such bonuses, but if driving yourself, you should check out this guide to great detours off the Golden Circle.Geysir,
Geysir is a famous hot spring in the geothermal area of Haukadalur Valley, found in south-west Iceland.
Making up just one of the attractions along the world-renowned Golden Circle sightseeing route, alongside Þingvellir National Park and the mighty Gullfoss waterfall, Geysir is most well-known for having lent its name to geysers all around the world.
Though Geysir itself is rarely active these days, Haukadalur Valley boasts a plethora of hot springs and geysers, including the powerful Strokkur, Smiður and Litli-Strokkur.
Strokkur is, arguably, the country's most famous hot spring, shooting vast jets of boiling water from 20 metres (65 feet) up to 40 metres (130 feet) high. Don't worry about missing this incredible spectacle of nature, as Strokkur erupts every five to ten minutes; just make sure to have your camera ready.
Geysir is much larger, but years can go by between eruptions here; it is currently in an inactive phase. When it does erupt, the water can shoot up in the air as high as 70 metres (230 feet).
Just a few minutes walk north of Geysir are a wealth of fumaroles emanating steam and gas into the cool Icelandic air. Aside from watching the hypnotic pillars of steam, you will also be able to observe the yellow sulphuric stains along the fumaroles themselves, a result of the earth's minerals crystallising around the rock bed.
At the southern part of the valley, Þykkuhverir, you'll find various bubbling mud pots. These spooky brown cauldrons are actually fumaroles that boil up through the loose ground; after a dry spell, these mud pools are likely to transform into a hardened fumarole.
Nearby Attractions
About two kilometres (one mile) from Geysir is a preserved natural pool called Kúalaug. It has room for three to five people at a time, but care should be taken, as the area around the pool is very delicate. The temperature is 39-43°C (102-109°F), depending on where you are positioned in the pool.
The water is slightly muddy, as the pool is built on soil, and the bottom is slippery due to algae, so caution is advised when relaxing here.
Haukadalur has also seen a rise in reforestation in recent times thanks to continued experiments and research in the area. Today, Haukadalsskógur is one of the largest forests in south Iceland, boasting accessible walking paths (also for wheelchair users), fascinating vegetation and The Tree Museum, built in the memory of forester Gunnar Freysteinsson.
Haukadalur has been inhabited and used as a church site since the Age of Settlement. Given its historic value, it should be noted that scholar, Ari "The Wise" Þorgilsson, grew up here; it was also where the first pastoral school in Iceland was built.
The current wooden church was last rebuilt in 1938 but its architectural style dates back to 1842, making it well worth a visit to see how Iceland looked before industrialisation.
For accommodation, Hotel Gullfoss is approximately 7 kilometre from the Geysir area, and closer still is Hotel Geysir on the other side of the road from the attraction, where you will also find a restaurant, café and a souvenir shop.Gullfoss,
Gullfoss (translated to 'Golden Falls') is one of Iceland's most iconic and beloved waterfalls, found in the Hvítá river canyon in south-west Iceland.
The water in Hvítá river travels from the glacier Langjökull, before cascading 32 meters (105 feet) down Gullfoss' two stages in a dramatic display of nature's raw power. This incredible site is seen by most visitors, as it is on the Golden Circle sightseeing route.
Because of the waterfall's two stages, Gullfoss should actually be thought of as two separate features. The first, shorter cascade is 11 metres (36 feet), whilst the second drop is 21 metres (69 feet). The canyon walls on both sides of the waterfall reach heights of up to 70 metres (230 feet), descending into the great Gullfossgjúfur canyon. Geologists believe that this canyon was formed by glacial outbursts at the beginning of the last age.
In the summer, approximately 140 cubic metres (459 cubic feet) of water surges down the waterfall every second, whilst in winter that number drops to around 109 cubic metres (358 cubic feet). With such energy, visitors should not be surprised to find themselves drenched by the waterfall's mighty spray.
As mentioned, Gullfoss makes up a part of the highly popular Golden Circle sightseeing route, alongside Geysir geothermal area and Þingvellir National Park. Many Golden Circle tours include additional activities that can be taken from Gullfoss, such as ascending the mighty nearby glacier Langjökull and entering its ice tunnels, or snowmobiling along its gleaming surface.
In the early days of the last century, Gullfoss was at the centre of much controversy regarding foreign investors and their desire to profit off Iceland's nature. In the year 1907, an English businessman, Howell, sought to utilise the waterfall's energy and harboured ambitions to use its energy to fuel a hydroelectric plant.
At the time, Gullfoss was owned by a farmer named Tómas Tómasson. Tómas declined Howell's offer to purchase the land, stating famously "I will not sell my friend!" He would, however, go on to lease Howell the land without the knowledge of a loophole that would allow him to proceed with his plans.
It was Tómas' daughter, Sigríður Tómasdóttir, who would lead the charge to stop Howell's ambitions. Having grown up on her father's sheep farm where she helped pave the first road to Gullfoss, she sought to get the contract nullified, hurriedly saving her own money to hire a lawyer.
The ensuing legal battle was an uphill struggle; the case continued for years, forcing Sigríður to travel many times by foot to Reykjavík, a distance of over 100 kilometres (62 miles). Circumstances became so difficult that Sigríður threatened to throw herself into the waterfall if any construction began.
Her tenacity, however, resulted in success. In 1929, Howell's withdrew from the lease, unable to keep up with the costs and difficulties of his plan. The waterfall fell back into the hands of the Icelandic people.
Today, Sigríður is recognised for her perseverance in protecting Gullfoss and is often hailed as Iceland's first environmentalist. As such, she is one of the most famous figures in Iceland's history. Her contribution is forever marked in stone; a plaque detailing her plight sits at the top of Gullfoss.
Interestingly, the lawyer who assisted Sigríður, Sveinn Björnsson, went on to go down in history too; he became the first president of Iceland in 1944.
Restaurant / Cafe
Besides Gullfoss, visitors can enjoy the views from Gullfoss Cafe, a locally run delicatessen that serves a wide variety of refreshments and meals. The menu has options to tantalise everyone's taste buds: hot soups, sandwiches, salads and cakes. There is also a shop on site where visitors' can browse and purchase traditional Icelandic souvenirs.Þingvellir,
Þingvellir National Park is the only UNESCO World Heritage site on the Icelandic mainland and one of the three stops on the world famous Golden Circle sightseeing route, alongside the Haukadalur Geothermal Valley (Geysir) and Gullfoss Waterfall. Just to the south of the park is Þingvallavatn, Iceland's largest natural lake.
The first thing that visitors to the National Park notice is its sheer aesthetic beauty; dried magma fields, covered in Icelandic moss, sit carved by glacial springs and surrounded by a bowl of ancient mountain peaks.
Two of the greatest attractions in the park are the exposed North American and Eurasian tectonic plates; it is one of the only regions in the world where you can see geology such as this on land.
Visitors are encouraged to walk along the North American tectonic plate, where they can read more about the area's fascinating formation and history, before descending into the valley below.
Þingvellir can be anglicised to 'Fields of Parliament', a nod to the area's fascinating history and relevance to Icelandic culture. It is here, after all, that the world's first democratically elected parliament that remains functioning, Alþingi, was formed in 930 AD.
It may seem unlikely that Vikings would want to be under such an uncombative government, but the thirty or so clans that lived in Iceland at the time sought to prosper in their harsh new environment.
The first gathering was such a success that the meetings became annual, and it became a place where disputes were settled, criminals were tried, and laws to the benefit of all were laid down.
This saw the birth of the Icelandic Commonwealth, a time of independence and freedom for the Icelandic people before they became constituents of the Norwegian monarchy. Sessions would continue to be held at Þingvellir until 1798.
Though the parliament was removed by the Danish at this time, it returned 1845 to Reykjavík.
Another major reason as to why Þingvellir is considered so important to Icelanders is the fact that this is where the decision was made to abandon the belief of Paganism and the Norse Gods; the people adopted Christianity in 1000 AD under threat of invasion from Norway.
This turning point in history was left to the pagan lawspeaker, Þorgeir Þorkelsson, who rested on the choice for one day and one night before reappearing to share his decision.
To symbolise the country's change, he threw idols of his old deities into the northern waterfall Goðafoss, the name of which translates to 'Waterfall of the Gods'.
Silfra Fissure
Þingvellir is one of the most widely visited attractions in Iceland, in large part due to the fact that it is home to the glacial spring, Silfra fissure, one of the top ten sites in the world for snorkellers and scuba divers.
Silfra (meaning 'Silver') is a submerged ravine within the park, boasting visibility of up to 100 metres (328 feet) and a temperature just above freezing. Participants in these tours will be attired at the Silfra carpark in neoprene hoodies and gloves, as well as an undersuit and drysuit for thermal protection.Kerið,
Kerið is a volcanic crater lake in the Grímsnes area of South Iceland. It is close to the three major sites that comprise Iceland's world-famous Golden Circle sightseeing route and thus makes for the perfect detour for those on visiting these landmarks.
Kerid and the Golden Circle
The Golden Circle is Iceland's most popular tourist route. Its three main highlights are Gullfoss, or the 'Golden Waterfall'; the Haukadalur Geothermal Valley, with its many springs and geysers; and Þingvellir National Park, the only UNESCO World Heritage Site on Iceland's mainland and the birthplace of the country's parliament.
There are several tour operators who offer trips to the Golden Circle. Many of those define their tours from others with extra stops, and Kerið crater is the most popular of these. It is approximately 40 minutes drive from Þingvellir and Haukadalur in different directions, providing visitors with two routes by which they can return to Reykjavík.
If travelling to Kerið crater alone, please be aware that there is a small entrance fee, of 400 ISK (about two Euros, or three US dollars). This is only to help the landowners preserve and protect the crater; after all, it is on privately owned land.
Other sites you may want to detour on when travelling the Golden Circle are the Sólheimar ecovillage, the Fontana Spa in Laugarvatn, the Secret Lagoon in Flúðir, and the Friðheimar tomato and horse farm.
Geology of Kerid
Kerið is approximately three thousand years old, making it roughly half the age of most volcanic calderas found in Iceland. This is the major reason as to why Kerið's slopes are red in colour, rather than a volcanic black; the iron deposits are, geologically speaking, fresh.
This vivid redness is part of the appeal of visiting this crater. The crimson rocks contrast dramatically with their surroundings, particularly the intense azure colour of the waters within the crater lake, and the verdant bursts of vegetation.
Kerið is approximately 55 metres (180 feet) deep, 170 metres (558 feet) wide and 270 meters (886 feet) in circumference. It is possible to take a path right to the crater's edge, to fully encircle it, and to descend down to the pristine waters of the crater lake.
Many crater lakes can be found in nearby, a region known as Iceland's Western Volcanic Zone. The area surrounding it is of barren, stark lava fields, further illustrating the volcanism of the region.
Scientists believe that Kerið was once a cone-shaped volcano. Upon eruption, it is widely believed that the volcano depleted its magma reserve, causing the foundation to fall in upon itself and result in the formation it has today. Of course, this was two thousand years before settlement, so no one is quite certain.
At some point in the centuries following the collapse, Kerið crater filled with water. The resulting lake is between seven and fourteen metres deep, depending on the time of year and the amount of rainfall.
The reason why the lake is so vividly coloured is that of the minerals from the rocks, seeping into and dyeing the water the aquamarine colour for which it is renowned.Friðheimar
Photo from fridheimar.is
Fríðheimar is a visitor-friendly tomato farm in south Iceland, renowned for its fresh produce, horse-breeding programme, and for the horse-shows that it puts on in fourteen languages.
Tomatoes at Fríðheimar
Due to the geothermal activity in south Iceland, there is boiling water just beneath the surface of the ground. This allows for natural hot springs, such as at Reykjadalur, for spa facilities at the nearby towns of Flúðir and Laugarvatn, and for the greenhouses at Fríðheimar flourish throughout the year.
Iceland, for centuries, had access to few fruits and vegetables, and usually only ate them over Christmas. This all changed when geothermal energy was used to power greenhouses. Now, much of the produce sold in stores in homegrown, and many of the tomatoes from Fríðheimar.
Visitors can tour these greenhouses, learning about this ground-breaking agricultural technique whilst tasting the produce available.
Horses at Fríðheimar
Fríðheimar breeds Icelandic horses and has six first-prize broodmares. The horses on site are used to put on shows that are fun for the whole family.
Icelandic horses are famously a very special breed. While most horses in the world only have four gaits – or ways of walking – these have five, being able to perform the tölt. This makes them very popular in dressage.
This breed of horse has other unique qualities, however. It is smaller and sturdier than most others, and also notably more intelligent and social. This is said to be because when early settlers arrived, they could only take their very best animals, and the resulting animals are from their descendants alone.
Sites around Fríðheimar
Fríðheimar's position in south Iceland means it is surrounded by an array of popular sites. The most famous and significant of these are no doubt those on the Golden Circle.
Þingvellir National Park, Gullfoss Waterfall and the Geysir Geothermal Area are all renowned for their beauty, accessibility to Reykjavík, and proximity to each other. Fríðheimar is often visited alongside a sightseeing tour around these, and can be combined with an array of other nearby locations for a full day out.
Start : 09:00.
Bądź w miejscu, z którego mamy Cię odebrać o wyznaczonej godzinie. Jeżeli znajduje się na przystanku autobusowym i potrzebujesz pomocy w jego znalezieniu, poszukaj wskazówek w recepcji hotelu, bądź skontaktuj się bezpośrednio z operatorem wycieczki.
Profesjonalny przewodnik
Wstęp do Kerið
Transfer spod hotelu (wybrane lokalizacje)
WiFi na pokładzie
Ładowarki USB
Wizyta na farmie Friðheimar i w szklarni
Posiłki i przekąski
Co zabrać:
Ciepłe ubrania
Porządne buty
Recenzje klientów
Brak recenzji / oceny w tym języku,


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Expert Advice on Produce Safety
Published June 23, 2015
Vegetables and fruits are popping up on more plates as the popularity of plant-based eating trends upward. As you enjoy produce (or more produce!), make sure it's safe to eat.
Fresh produce doesn't get a free pass when it comes to foodborne illness. It needs to be handled properly, like any food. According to the U.S. Food and Drug Administration, there were approximately 131 produce-related reported outbreaks from 1996 to 2010, which led to 14,350 illnesses, 1,382 hospitalizations and 34 deaths. But foodborne illness associated with produce is mostly preventable.
From farm to fork, food safety contamination due to harmful bacteria can occur along all stages of a fruit's or vegetable's journey, including during growing, harvesting, manufacturing, processing, packing, holding or transporting. After that, you take over most of the food safety responsibilities, beginning at the store. Follow these tips to help ensure your produce is safe to eat.
At the store
- Pick produce that is free of bruises and other damage; select pre-cut fresh produce that's refrigerated or encircled by ice.
- Keep fresh fruits and vegetables separate from raw meat, poultry and seafood products in your shopping basket, at the checkout lane and during bagging.
- At an outdoor market, especially during hot weather, shop early in the day to get a better selection of produce that is fresh and not overhandled.
On the way home
- Go straight home after your supermarket or farmers market trip.
- If unable to go directly home, bring a cooler with ice packs to keep cold food cold.
- On warm-weather days, transport groceries inside an air-conditioned vehicle rather than a hot trunk, when possible.
At your home
- Don't leave fresh-cut, peeled or whole perishable fruits or vegetables, such as strawberries, lettuce, and herbs, in a hot car.
- Immediately store fresh-cut, peeled or whole perishable produce in a clean refrigerator set at 40°F or below.
- If produce was refrigerated at the store, it generally needs to be refrigerated at home.
During food prep
- Wash all fruits and vegetables (even if you plan to peel them) thoroughly under running water before cutting, cooking or eating. Dry with a clean cloth or paper towel.
- If the package indicates the contents are pre-washed and ready-to-eat, you can use the produce without additional washing.
- When produce is to be eaten raw, keep it separate from other foods, especially raw meat, poultry or seafood, by using clean and separate cutting boards, cooking utensils and prep dishes.
- If damaged or bruised areas are found on fresh fruits and vegetables, cut them away before preparing or eating. Throw out anything that seems rotten.
Reviewed June 2015 Jackie Newgent, RDN, CDN, is a Brooklyn-based culinary nutritionist, writer and media personality.


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Subscribe To Watch Ryan Gosling Sell Knives With Will Ferrell On Jimmy Kimmel Live Updates
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In anticipation of the release of Ganger Squad, Ryan Gosling appears to be making the late night talk show rounds. That includes a visit to Conan, during which he gave an audience member the opportunity to take his place in the interview chair, and an even less conventional appearance on Jimmy Kimmel Live. Check out both videos ahead.
First up, Gosling plays a crucial supporting role in a bit for Jimmy Kimmel Live that features a certain beloved Anchorman, who has some really special knives to sell.
"Your energy did this," Gosling tells Jimmy, when Ferrell gets hurt during his knife slashing (slamming?) demonstration.
I think it's great that he pushes the blame for Ferrell's injury onto Kimmel, while complimenting Ferrell on how beautiful his cut is. He really does put the 'support' in supporting role, even if it's just for a late night talk show comedy bit. Or maybe I just find everything Gosling does to be irresistible and adorable, even if he's just standing there holding a tray while Will Ferrell pounds it mercilessly with a sword.
And speaking of adorable, here's the video of Gosling's appearance on Conan, which begins with him saying he was once an audience member at a late night talk show. The lead-in to the segment is a little awkward at first, but things warm up when an audience member is brought up on stage to serve as Gosling's interview buddy. All of this leads up to a clip from Gangster Squad, which arrives in theaters this weekend.
Conan's great at taking jabs. It's part of his shtick, and Gosling's sidekick did a find job of delivering her lines, which lead up to the clip for Gangster Squad (in theaters today!).


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|By Keith Mayer
Article Rating:
|May 13, 2013 09:00 AM EDT
This month, my fellow IT Pro Technical Evangelists and I are authoring a new articles series on 20 Key Scenarios with Windows Azure Infrastructure Services. You can catch the full series at http://aka.ms/CloudTop20. In today's article in this series, we'll discuss extending our On-premises Datacenter into a Hybrid Network Infrastructure with Windows Server 2012, Windows Azure Virtual Networks and Site-to-Site VPN Tunnels.
Windows Azure Virtual Networks allow us to securely extend our Private Cloud with a hybrid network infrastructure that leverages the best of two worlds: the low-latency and high-bandwidth available with our on-premise datacenter networks with the elastic, pay-as-you-go cloud model of Windows Azure Virtual Networks and Virtual Machines.
Windows Azure Virtual Networks – Guided Lab Scenario
Windows Azure Virtual Networks offers the power to open up several cross-premises use case scenarios, including:
- Active Directory Disaster Recovery
- SQL Database Replication
- Windows Server 2012 DFS-R File Replication
- Accelerated Cloud File Services with BranchCache
- Hybrid Web Applications
- and MORE!
But … we need to configure the Virtual Network FIRST … which is what this Guided Hands-on Lab Scenario is all about!
Once configured, a Windows Azure Virtual Network gives us the power as IT Pros to pick where each application workload is hosted – either on-premises or in Windows Azure – based on the network, storage, security and Internet requirements of each application. However, as an interconnected hybrid scenario, we can continue to manage VMs centrally with System Center 2012 SP1 and Windows Server 2012 Server Manager, regardless of where those VMs may really live.
Guided Lab Exercises
In this Guided Lab Scenario, you'll complete the end-to-end configuration steps for building:
- Windows Azure Virtual Network and IP Address Space
- Virtualized Subnets for VM and VPN Gateway workloads
- Site-to-Site IPsec VPN Tunnel for secure cross-premises connectivity
- Virtual VPN Gateways in Windows Azure
- On-premises VPN Gateway using Windows Server 2012
Once you're done, you'll test the configuration by placing a Windows Azure Virtual Machine on your newly provisioned Virtual Network.
Estimated Completion Time: 15 to 20 minutes
Let's Get Started!
To launch this Guided Lab Scenario, click the Play button in the window below.
Guided Lab Scenario: Windows Azure Virtual Networks
Guided Lab Tips:
- After launching the lab in the window above, you may wish to also click the Show Full Screen button in the lower right portion of the window for best viewing.
- The lab captions can be moved by clicking/dragging if they obscure a portion of your Guided Lab window, particularly when using lower screen resolutions.
Get the associated 48-Page Step-by-Step Lab Guide for FREE ...
If you'd like a copy of the 48-Page Step-by-Step Lab Guide that is associated with this Guided Hands-on Lab for reference when building our your own virtual networks ... just follow the easy steps below to get it sent to you!
You'll receive an email in response with your 48-Page Lab Guide Attached!
Completed! Now what can I do with a Virtual Network?
Now that you know the steps for creating a secure cross-premises Virtual Network with Windows Azure, check out the following hybrid network scenarios that can leverage this Virtual Network as part of your Private Cloud infrastructure:
- AD Disaster Recovery: Extend On-Premise Active Directory to the Cloud
- File Services: Accelerate the Cloud with Windows Server 2012 "BranchCache"
- Web Applications: Connect PaaS and IaaS Applications in the Cloud
- Dev, Pilot, Test Environments: Templating VMs in the Cloud with Windows Azure
And ... while you're at it, be sure to check out the rest of our recent blog article series at:
- READ IT: Build Your Private Cloud in a Month
- READ IT: 20 Key Scenarios with Windows Azure Infrastructure Services
Are you using Windows Azure Virtual Networks?
Do you have interesting use cases or scenarios that you're delivering with Windows Azure Virtual Networks? Feel free to leave your comments and feedback below to share across our IT Pro community!
|Build Your Lab! Download Windows Server 2012
|Don't Have a Lab? Build Your Lab in the Cloud with Windows Azure Virtual Machines
|Want to Get Certified? Join our Windows Server 2012 "Early Experts" Study Group
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Making Mead at LBC part 2,3,4
Getting ready to lift the lid off of these very tight buckets. I got this equipment form City Brewshop. Brew. Drink. Repeat.
After the initial pitching of the yeast and the initial adding of the nutrients and energizer, you have to stir the mead 3 more times and add the nutrient/energizer combination each time. I started out with the mead in the kitchen, along with my Petite Saison d'Été. The weather had been lovely and cool. Then it got hot. The kitchen soon became a very bad place for the mead and beer. In the middle of the day, the temperature rose to around 80 degrees. I moved the three 5 gallon buckets to the cellar where it ranged from 63.5F to around 67F. The mead and beer is very happy down there. I don't think it ever gets above 70 degrees in the cellar. At least, I hope not. I don't have any more places to move it. I wish I had some dry ice, I could always throw that in. It's an old wine making trick.
The golden mead. I used a lot of honey on these two buckets.
First I stir to release the built up CO2. When I did this the first time, the mead, the Dry Mead, actually hissed at me. And then when I threw in the nutrient. It really bubbled up.
Not as energetic the second time. This is from the second day. Or it may have been the third day.
Weighing out the nutrient mix I made up.
Now adding the mixture.
And as you can see, it's starting to foam up.
Stirring. This is the Sack Mead, which isn't quite as energetic as the Dry.
This is the Dry Mead and as you can see, it's bubbling like crazy.
Wow. Will you look at that head. I had to do this once a day for 3 days. I had to sanitize all my equipment and it was a little bit of a pain doing it in the cellar but I managed. The Sack Mead is supposed to be like a great Ice Wine and the Dry Mead, which I'm going to bottle condition and make bubbly is probably like nothing I've ever had. We'll see in 3 months for the Dry and 4 months for the Sweet Sack Mead. I can't wait.
Labels: beer brewing, Beer Recipe, life by chocolate, mead, Recipe


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Rock Music City!
Run a scenic course through the heart of Nashville and check out Broadway, Music Row, the Gulch and more as you rock your run!
Bucket List Race
Book your trip to Nashville and check this destination city, with an iconic course, off your list!
Get ready to rock out with music all weekend! St. Jude Rock 'n' Roll Nashville is known to bring in top tier headliners year after year! Stay tuned for your 2020 headliner!


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A Millyview and ramble inspired by the book of Hosea and its reflection of God's steadfast love for his Covenant People then and now,
There was nothing noble about your definitions.
Fickle were your affections,
Factional were your motivations,
You were a disgrace to the nations,
Maybe you didn't see anything wrong with your actions,
It's the system of the world: all the same in its prescriptions,
You say you needed provisions for your various ingestions,
no conception…
To BAAL-peor was the praise forever on your meditations,
Building empires, towers, metal images and crouching in genuflections,
Turn away from your whoredom,
Lest you die,
Jesus came down into your nation,
New Covenant drafting,
His sacrifice was beyond comprehension,
The passion,
Jesus came that you might live after resurrection,
Eternity in mind,
Though you were yet a sinner he offered reconciliation,
So divine,
You were a WHORE, but the Lord has called you
Would you live as though….YOU KKNOW?
Hosea 2:19Amplified Bible (AMP)19 "And I will betroth you (Israel) to Me forever; Yes, I will betroth you to Me in righteousness and in justice, In loving-kindness and loyalty, and in compassion.
~Beloved,Stay pure~
2 Corinthians 11:2Amplified Bible (AMP) 2 I am jealous for you with a godly jealousy because I have promised you to one husband, to present you as a pure virgin to Christ.
Are we the body of Christ breaking our bridegroom's heart?
Are we spiritual virgins ready for our consummation when Christ returns?
Or have we allowed this world to rape us? With its continuous distractions personal and afar! From ideologies that completely disagree with His truth, with the pursuit of more knowledge that doesn't glorify your name, to the Presidential elections as if they could bring any hope?
Or worse willfully relinquishing our covenant; rejecting our bridegroom!!! Your judgement is coming lest you turn from this way: REPENT!!!
To those who have called Jesus Lord have we been pure in our affections? Or have we been playing the whore? Seeking to serve the works of our hands as our Gods or living a meaningless life filled with all pleasures that leave us empty? Have we ceased to listen to his voice? To have his heart? How this breaks mine to think about 😦
What must we do to ensure that when our bridegroom returns for us we are found ready?
We who have sipped from his cup are now in our preparation, we therefore must put on our righteous garments and cultivate a Godly life that is pleasing to him even whilst we wait on Him to return and draw us home.
Revelation 19:7-8Amplified Bible (AMP)7 "Let us rejoice and shout for joy! Let us give Him glory and honor, for the marriage of the Lamb has come [at last] and His bride (the redeemed) has prepared herself." 8 She has been permitted to dress in fine linen, dazzling white and clean—for the fine linen signifies the righteous acts of the saints [the ethical conduct, personal integrity, moral courage, and godly character of believers].
Ephesians 5:10-11 Amplified Bible (AMP)10 trying to learn [by experience] what is pleasing to the Lord [and letting your lifestyles be examples of what is most acceptable to Him—your behavior expressing gratitude to God for your salvation].Do not participate in the worthless and unproductive deeds of darkness, but instead expose them [by exemplifying personal integrity, moral courage, and godly character]
The Lord is calling you!! No matter what you've been through in this life he longs to forgive you of your sin, to have a personal, intimate relationship with you and to work in you regardless of the list of your sin whether you count it as such or not. Whether you were born nobly or belong to high society or not, to God you are very precious, more than you know. This is not religion this is a way of life this is laying aside all that you previously regarded and following Christ!! The best relationship you could ever enter. So surrender to this resurrected life, that you may be spiritually alive!!!
Many of us try to understand these things with our human minds, but it requires a Holy Spirit perception and a continually renewed mind to point us rightly to God: what spirit informs you if not the holy one? With the Holy Spirit we are taught daily to rely fully on God for wisdom, for our brains are limited and his ways are far above ours,
He is ALL sufficient!!
His kingdom is not of this world but he longs for us to start living as citizen's of such even now so rise up oh betrothed that you are and serve him faithfully. My President is GOD the ISHI of the Church!!!
Remember one who is betrothed to another represents the other's reputation.
Walk into his promises and represent him well oh Bride of Christ!!
Hosea 14:9 (ESV)9 Whoever is wise, let him understand these things; whoever is discerning, let him know them; for the ways of the Lord are right, and the upright walk in them, but transgressors stumble in them.
Stay Faithful, Be HoLy
Be One
Ephesians 4:12-13(AMPC)His intention was the perfecting and the full equipping of the saints (His consecrated people), [that they should do] the work of ministering toward building up Christ's body (the church), [That it might develop] until we all attain oneness in the faith and in the comprehension of the [[a]full and accurate] knowledge of the Son of God, that [we might arrive] at really mature manhood (the completeness of personality which is nothing less than the standard height of Christ's own perfection), the measure of the stature of the fullness of the Christ and the completeness found in Him.
~Feel the weight of it~


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My Stuff
Sailing to Rarotonga
a travel blog by
Shellback Fire Coral
Bob-Kate joins the Barque Picton Castle to sail the wide accountantsea. From Lunenburg, Nova Scotia to Rarotonga, Cook Islands via Panama, Galapagos, Pitcairn & Tahiti
view 1 photo from this trip
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written by
Shellback Fire Coral
on May 1, 2010
from the travel blog:
Sailing to Rarotonga
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The Atlantic
George Hill
The first of the seven seas is crossed as we prepare to slip into the Caribbean Sea from the squally Atlantic. Anguilla is to be our first port of call since Nova Scotia; we're due to arrive on Monday morning (I'm writing at 13:28 ship's time on Saturday 22nd May). The news that we are to stop in the Caribbean en route to Panama was posted during our 8-12 watch this morning and caused much excitement after a fortnight at sea and the prospect of another 10 days or so to Panama.
The Atlantic crossing went well, with just one "Goldilocks gale", not too big, not too small, just nice and breezy, and exactly long enough for the excitement to wear off and the flat seas and sunshine to be welcomed by all hands with delight once it had passed. It seems Neptune was quickly appeased, a good omen for the voyage.
Our course has been almost due South, with a few points first to East and then to West to position us on the advantageous side of a couple of low pressure systems. We've been able to sail most of the time with a small amount of "iron topsail" to keep up our progress South when the wind wasn't cooperating. We are a very pretty sight with all square sails set, the t'gallants especially have a lovely full shape when set just right, and the royals seem to be as much a part of the sky as the ship. We've had a couple of yachts come closer than strictly recommended by the collision regulations to take a closer look, but who can blame them?
As our latitude has dropped off from 45°N in Canada down through the thirties and twenties, the clothes have been also falling off the crew. The 15 minute layering ceremony of thermal underwear right up to waterproofs and woolly hat has been replaced by a shorts, t-shirt and sun block routine, although foulies still make an appearance in the squally moments especially at night. The water temperature has been building pleasingly from 4°C in Lunenburg to a respectable 16°C or so once we hit the wonderful Gulf Stream and is now a tropical 29°C. This was considered good news until the Cap'n explained that 29 degrees is the magical temperature above which hurricanes can form from a tropical low…I have to admit to watching with slight trepidation for each incoming squall just in case it's a tiny hurricane that somehow escaped our satellite weather forecasting system and radar.
All hands have now settled into the routine of their watches, it's an easy 4 hours on, 8 hours off with the different watches having ship's work to keep them busy as well as sailing the ship. Rust busting, cleaning, helping Donald (our awesome cook), tarring, painting and greasing are all now as much a part of life as steering, keeping look out, running up aloft to furl or loose sails, and handling lines. We are starting to look a little more like sailors and less like dyspraxic geriatrics as we find our sea legs and to stare a little less blankly when the mate of our watch shouts out an order: "hands to the main braces" now only usually has one or two of our watch accidentally running for the fore sheets.
Today was a milestone on the Aloha deck: our first fish! Congratulations to Julie and Dan with Logan's patented bungee line. It was a respectable size, but the Mate sagely insisted we return it to the Pescatorial Gods to improve the future catch. We also had a suicidal flying fish stun itself on Port side of the scullery, first flying fish goes to the Cap'n and the second to the ship's cat, or is it the other way around?
So now the thought of rum and ice creams on a Caribbean beach is making for a party atmosphere on board, as people top up their tans to make ready for bikinis and dig out their shore clothes in anticipation of "cultural experiences" ashore.
The next time someone suggests sailing South to find the tropical trade winds I think I already have my answer.
written by
Shellback Fire Coral
on June 27, 2010
George Hill
from the travel blog:
Sailing to Rarotonga
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Caribbean Cruising, Panama and the Realm of King Neptune
Puerto Baquerizo Moreno
Anguilla and Bonaire were our two Caribbean Island stops, both wonderful but with a very different feel. Anguilla was the perfect beach island, populated mostly by honeymooning couples. The party each night was at Elvis´s beach bar but that's mostly because the crew of the Picton Castle was drinking rum punch there and dancing on the beach. The people of Anguilla were crazy friendly; hitchhiking was just a matter of walking for 2 minutes out of town looking hot and tourist like until some kind person stopped to offer a ride. Once they heard we were from the big ship anchored off in Sandy Ground, almost everyone went out of their way to drive us to whichever beach, post office or bar we were heading for. The weather was hot hot hot, so we spent some time swimming and snorkelling to keep cool, and the roadside goat curry was epic.
Bonaire was much bigger and more touristy. We were also docked rather than at anchor, so crew could come and go at any time of day or night, convenient but less restful. The dock came in handy though, for sewing a mainsail for a boat called the Mermaid, the Captain being a friend of Captain Moreland and the boat needing a mainsail. This was good fun if quite frustrating as our crazy ancient sewing machines struggled to sew the heavy, stiff Dacron. I´m told sewing our own cotton canvas sails is much easier, so looking forward to when the sail making starts for the ship. The whole operation was under the command of the third made, Rebecca, who learned sail making on the Picton on her first world voyage. Flamingos were a Bonaire highlight for me – we hired a Jeep (held together with duck tape and its own soft top) to get around the Island, and drove into the hills where a huge lake is home to the awesomely pink birds. They look like they're dressed up for a night out. We had a short windsurfing lesson, which was wicked fun, especially when Georgie and I managed to sail one of them tandem, me surfing on the back of her board. The nightspot was a groovy bar called little Havana, complete with some people other than the crew (shocking to see new faces) and excellent Mojitos.
Our next anchorage was off Colon in Panama, we had not cleared in through immigration so we stayed aboard except for a visit to a shiny yacht club which apparently didn't count as ashore to the authorities. My favourite thing here was watching all of the ships at dusk and identifying all of the different navigation lights for specific vessels: white over red for pilots, red white red for dredgers, all these things I've never noticed except in books before. The canal crossing was more memorable for the sense of history then the actual crossing, although it was fun, and the locks bigger than I´ve ever seen, most of the work was done by professional line handlers and big train things that shunt ships through the locks. Lake Gatun was pretty spectacular with jungle right down to the water. No crocodiles sadly. Glad some of you managed to see us on the webcam, thanks for watching! Panama City was fun and rushed. We were on a dock on the Isle de Flamincos, an island connected to the mainland by a long causeway. Days were mostly spent manically shopping for presents for the families we will stay with on Pitcairn, as well as some supplies for the voyage anda small amount of treasure. There was also a fair amount of wandering around the old town, which looked like a South American version of Spain, and some dancing at Mojitos sin Mojitos, an excellent bar with no roof, its walls just the terraced buildings either side.
I am writing to you Pollywogs all as a Royal Shellback of the Court of King Neptune. Our equator crossing ceremony yesterday was epic, complete with Davey Jones, Aphrodite, Seamonsters galore and of course King Neptune himself. His trident was even used for a spot of pole dancing at one point. My sin against the Deep was being too white (fair, but rectified with tar pretty effectively), and my new Shellback name is Fire Coral, which I think is rather wonderful.
The sail from Panama was good, if short, with the Southern Cross appearing to show us the way, so now a few days to explore Galapagos and then nearly a month at sea before Pitcairn.
written by
Shellback Fire Coral
on June 27, 2010
Puerto Baquerizo Moreno
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Sailing to Rarotonga
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Hi Guys!
I have a few of the latest launches from MAC to share with you today – starting with their rather exciting Rollerwheel Eyeliner…
You've probably heard about the Rollerwheel Liquid Eyeliner (£16.50) or 'pizza cutter' eyeliner by now, as it's been talked about quite a lot recently. This completely new and innovative approach to eyeliner has got beauty fans across the globe excited – the idea being that a teeny tiny wheel is ran across the lash line to give a perfectly even and straight line – but does it actually work?
Well, rather like the Benefit They're Real Push Up Eyeliner, which was quite a weird and wonderful concept at the time of launch, the roller wheel does take a bit of getting used to. The tiny spinning disc does give a nice sharp line, which is very fine indeed, and would take most people quite a few goes to get it to a decent thickness. However, if you like a super thin line applied close to the lash line, this could be the product for you. Be prepared to practice though as the applicator does take a bit of getting used to.
This is available in Black, Brown and Blue, in both matte and shine finishes.
Next I have the new Bold & Bad Lash Mascara (£19.50) – this mascara is basically two products in one. It has a dual chamber which contains a large brush for the upper lashes, and a mini brush for lower lashes – with each having different mascara formulas to suit the upper and lower lashes too.
The formula promises to do everything you'd expect, apart from being flake proof and smudge proof, the larger brush delivers customized volume to the top, whilst separating the lashes, while the smaller brush creates smudge-proof definition to the bottom.
Though personally my super short lashes find the large main brush a bit on the big side, I love the mini brush for the bottom lashes, and the formula is fantastic too.
And lastly I have a few of the new limited edition Metallic Lipsticks to show you. There are 18 shades to choose from offering rich colour with smooth pearlized chrome finish, in a wide range of colours. The 18 shades are as follows –
- Royal Hour – a deep royal purple with blue pearl.
- Foiled Rose – a brightened blue pink.
- Hades Fire – a cool red burgundy.
- Silver Spoon – a multidimensional silver.
- In Lust – a light warm neutral.
- Jupiter – a bronzed neutral.
- Pearly One – a confetti'ed white frost.
- Disobedient – a deep burgundy red wine.
- Anything Once – a blackened blue with multi-colored pearl.
- Metalwork – a pale beige with golden shimmer.
- Love's A Gamble – a light pink with peach and silver shimmer.
- Rose-Dipped – a mid-tone pink with gold and silver shimmer.
- Wild Nectar – a rose with silver shimmer.
- Optix – an orange with gold pearl.
- Ionized Iris – a bright fuchsia with multi-color pearl.
- Forbidden Romance – a brick red with multi-dimensional pearl
- Pale Rose – a cool muted neutral pink.
- Cold Front – a frosted periwinkle blue.
I have a few of the more out-there shades, as well as a more wearable pink, swatched from top to bottom above –
- Anything Once – a blackend blue with multi-colored pearl.
- Rose-Dipped – a midtone pink with gold and silver shimmer.
- Royal Hour – a deep royal purple with blue pearl.
- Cold Front – a frosted periwinkle blue.
You can see all the shades offer decent pigmentation, but are still on the sheer side. If you're looking for full coverage then these are not for you! However, they do look great worn on top of a liquid lipstick, which gives a nice base to set off the shimmer and sparkles.
All the above products are available to purchase now at MAC and Selfridges.
I hope you enjoyed my post xxx
This post contains press samples
Beauty Collection Eyeliner lipstick MAC Makeup mascara Review


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AHS (AHS) Class of 1992 Alumni List
Help us Update the AHS 1992 Class List by adding any names missing from the list below:
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1992 Class List
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The top movie in theaters during 1992 was Aladdin, grossing over $217,350,219 in its lifetime. The most popular television series for 1992 was 60 Minutes
Hit Songs From 1992
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Reunions and Arlington High School News
More 1992 alumni from Arlington HS Arlington, NE have posted profiles on Classmates.com®. Click here to register for free at Classmates.com® and view other 1992 alumni.
Missing some friends from Arlington High that graduated with you in 1992? Check the list below that shows the Arlington Arlington, NE class of '92.
For former Arlington students from NE, you can view the class reunion information that has been posted by other site visitors. In addition, we encourage you to post your profile in the alumni directory, share information about the school activities you were involved with and reconnect with Arlington High School alumni.


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Below are five noteworthy communications stories from the week ending February 9, 2013.
"Age, Gender Determine 'Go-To' Devices" on eMarketer website, February 4.
As this article explains, TV in 2013 is still the go-to source of news and entertainment for most Americans, according to a new study by Harris Interactive. But young adults—those between 18 and 34—are quickly turning away from TV and relying instead on their laptops and smartphones.
Charts in this article show the percentages of different age groups moving to new devices and those considering replacing their computers with tablets.
"Nine Writing Mistakes You're Probably Making" by Ben Yogada on The Huffington Post, February 5.
Yogada, the author of How to Not Write Bad, says that for writing, it's the best of times because so much writing is being done and it's the worst of times because much of this writing is bad.
He lists nine writing mistakes and explains how to correct them. The first mistake, for example, is being wordy. By "wordy," though, he does not mean writing long sentences. He means using words that should be omitted.
"Pentagon gearing up to fight the PR war" by Walter Pincus, in The Washington Post, February 6.
In this informative article, Pincus says that although public relations (referred to as Inform and Influence Activities) is not new to the military, the U.S. Army is now embracing PR as a key element of its 21st-century military operations. He quotes the new Army field manual as stating PR is critical in ". . . leading operations toward attaining the desired end state," and that "Victory depends on a commander's ability to shape, sway, and alter foreign audience perceptions, and ultimately behavior, especially in the area of operations."
These objectives would fit into almost any good PR campaign.
"The Peculiar Twitter Tactics of Social Media Influencers" by Haydn Shaughnessy, on the Forbes website, February 7.
Shaughnessy, suggests in this post that Twitter has "become the channel for the new motivational micro-speech," leading the trend in social media to provide readers with inspiration and motivation. He says there are "social media influencers whose tweets and interactions are regularly interspersed with homilies," and he gives interesting examples.
"10 Tips From Boing Boing On Making Online Content Sing" by Camille Sweeney and Josh Gosfield in Fast Company, February 8.
Sweeney and Gosfield are the authors of The Art of Doing: How Superachievers Do What They Do and How They Do It So Well. In this article, they excerpt from their book ten tips for building an addictive, compelling website. Their tips come from Mark Frauenfelder, founder of the online magazine Boing Boing, which has been published since 1995 and has 2.5 unique visitors a month.
The tips provide good advice. For example, the second one—be original—says, "Make the blog that doesn't exist yet, but that you'd want to read."


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Dharamsala Trek
Delhi, India, Southern Asia, Asia
"Mountain views for miles combined with the infectious rich culture of Northern India - this trip is definitely an eye-opener for a number of reasons!"
From hill stations and shepherd settlements to high Himalayan mountain camps, this tour combines scenic trekking with superb sightseeing to create an altogether unforgettable experience. North of the Punjab plains, the southernmost ridges of the Indian Himalayas are known as the Dhauladhar, or White Range. Rising to around 5,000 metres, the British established several hill stations amongst these peaks; your tour begins with visits to the charming Dharamsala and McLeod Ganj, two such hill stations. McLeod is the current seat of Tibet's popular leader, the Dalai Lama, and is also home to a large community of Tibetans.
After exploring this vibrant and colourful pilgrimage destination, trek through delightful villages and high pastures, following in the footsteps of the shepherds who have used these trails for hundreds of years. Making a gradual ascent, you'll walk for around four hours per day, giving you plenty of time to interact with friendly villagers and learn more about their culture and customs. Admire fantastic views of the high peaks and rugged ridges of the Dhauladhar range from scenic campsites. Continue your tour with a visit to Palampur, northwest India's 'tea capital', and a night's stay in a 300-year-old manor house. Finally, conclude your adventure with a stunning drive through the Himalayan foothills followed by an overnight train journey to Delhi.
We understand that tourism often helps to alleviate poverty and develop communities and, as part of the global tourism industry, acknowledge that they too have a crucial role to play in providing commercial benefits and financial impetus in local communities. The company is conscious of both the negative and positive impacts its operations potentially have within sensitive environments and communities, particularly in the Himalayas where most of our trekking and adventure operations are conducted. To ensure that they operate in as responsible a way as possible, we train all of its tour leaders and support staff on sustainability practices. One example is their 'Leave no Trace' policy, which ensures that campsites and trails are left free from any waste. The company even extends this policy to include the waste left by travellers who have come before them, collecting and disposing of any extra rubbish in a safe manner.
Arrive at Delhi's international airport, and receive a traditional 'swagat' (welcome) from Active India's representative. In the evening transfer to the railway station to board the overnight train to Pathankot. This is an experience in itself – move from the crowds and chaos of Delhi Railway Station to the rustic scenes of countryside life in Pathankot.
Arrive in Pathankot where you'll meet your drivers and vehicles to begin the next stage of your journey. Drive into the Himalayan foothills towards Dharamsala and the Tibetan Buddhist settlement of McLeod Ganj. Check in to your hotel in McLeod before enjoying an afternoon walking tour of the town. Visit the Dalai Lama's complex, St John's Church and the Tibetan Library. In the evening, wander through the bustling bazaar and soak up the atmosphere.
Today your trekking adventure begins! Walk from McLeod Ganj to Kareri village, a gentle ascent that takes you through traditional Gaddi shepherd settlements and terraced rice and barley fields. Set up camp next to the river and a watermill, and then enjoy the rest of the day to explore the village at your leisure.
After breakfast, take a gentle walk from Kareri to Barlai village (2,475m), ascending gradually through rhododendron forests and fields. The forests are home to bears, pheasants and even leopards (although these elusive creatures can only be found in the most remote areas of the forest so it's unlikely you'll see any). Stop for a relaxed lunch before completing your walk to the campsite at Barlai village.
Today is a little longer than your previous walking days, but is even more spectacular. Walk through a woodland reserve overlooking the stunning Kangra Valley to reach the village of Dharamkot (approximately 2,000m). The village is inhabited by Gaddis, and was also the retirement home of the English artist Alfred Hallett, whose paintings are still exhibited in the village!
Continue to the highest point of your trek at Triund (2,850m). The trail from Dharamkot leads you steadily uphill through rhododendron and deodar forests, until you reach a ridge. Here, enjoy beautiful views of the snow-capped mountains of Dhauladhar. The panoramic vista extends from the Kangra Valley all the way down to McLeod Ganj. You'll camp overnight on the ridge.
For the final part of your trek, return to McLeod Ganj. The walk takes around three/four hours and is mostly downhill. After lunch, continue with a drive to Andretta, a small Kangra village known for its pottery. Located in a beautiful Himalayan setting, its meditative environment provides plenty of artistic inspiration, from the surrounding snow-capped mountains to vast plains in the distance. Visit a studio to see the potters working and selling their wares. You'll stay overnight in a restored private house.
Today you'll drive down into the town of Palampur, the 'tea capital' of northwest India. Visit the surrounding tea gardens and learn about production processes before enjoying lunch. This hill station is not only known for its tea gardens and paddy fields, but also its colonial architecture and temples. In the afternoon continue to Pragpur, a 'World Heritage Village', by road. Check in to your accommodation at the 'Judge's Court', a 300-year-old manor house set in its own gardens, and travel back in time to the days of the Raj.
After breakfast, spend a free morning relaxing or exploring the local area. Pragpur, with its cobbled streets and stone and mud houses, has remained relatively unchanged for a century. In the afternoon, take a scenic drive through the Himalayan foothills to Pathankot where you'll board the overnight train to Delhi.
Arriving in Delhi in the morning, you'll transfer to the hotel to use day-rooms to freshen up. Spend the rest of the day doing some last-minute sightseeing, before later transferring to the airport to board your onward flight.
Rate Conditions
- The rate shown is a 'from' price per person (on a double sharing basis)
- Click 'Ask a Question' up above to speak to the operator directly about this tour's departure dates, availability and costs
- Refunds: 80% refund available until 30 days prior to check-in; 50% refund available 15 days prior to check-in; 25% refund available 6 days prior to check-in; within 5 days of check-in there will be no refund.
- Accommodation
- 1 x overnight train journey
- Breakfasts only (apart from during the camp stay, which will include full board meals)
- English speaking monument sightseeing guides at cities
- All land transport
- Government service tax as applicable
- Travel insurance
- Indian visa fees
- Any monument entrance fees
- Flights (domestic/international)
- Any optional activities or services not mentioned in inclusions
- Services of any professional English speaking tour leader/escort during the tour
- Miscellaneous expenses for tips, laundry, drinks, any medical emergencies and souvenirs etc.


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listen to the finished version of Maggie Rogers' "Alaska"
It's fun when a song that has been so incessantly hyped — and was, by any measure, already a hit before it was even finished — ends up being as good as you'd hoped. Maryland's Maggie Rogers shares the beautiful finished version of her undeniable viral sensation "Alaska", on which she manages to organically combine her impressive, more traditional folk music background with the "energy of dance music" to create a pulsing, heartfelt pastoral-dance-pop gem that reminds me of the time Amelia Randall Meath transformed her former group Mountain Man's a capella folk hymn "Play It Right" into a full-on Sylvan Esso banger. Listen below, synced with the Skateboard P gif for full effect:


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Liverpool will make a move for Leicester City midfielder Wilfred Ndidi, according to reports from journalist Alpha Balde.
The Guinean reporter posted on Twitter to claim that Liverpool have made an approach from the Nigerian international, with the player himself apparently keen on the switch to Anfield.
If the reports are true, Liverpool are yet to reach an agreement with Leicester about signing their midfielder. The Foxes may demand a high price for Ndidi, who has become a key player in Claude Puel's side and is consistently rated as one of the finest players of his age group and position in the world.
Ndidi approché par Liverpool. Le joueur aurait donné son accord. Reste à se mettre d'accord avec Leicester qui demande beaucoup. #LFC @LFCroundup— Alpha BALDE (@AlphaBalde91) March 18, 2018
Ndidi signed for Leicester in January 2017 from Belgian Pro League side Genk. The Foxes paid £17m for the 21-year-old, who has taken up a defensive midfield role in Leicester's starting lineup. He has adequately filled the place of N'Golo Kante, who left Leicester for Chelsea in 2016.
This season, Ndidi has completed 125 tackles in the Premier League, more than any other player in the division. Contracted to the King Power stadium until 2022, the Foxes will be reluctant to lose another key midfielder to a Premier League rival.
Wilfried Ndidi is such a talented player. Wins the ball back with ease.— John Bennett (@JohnBennettBBC) March 18, 2018
Huge potential; only 21 and only been playing in that position for about 3 years. Will keep getting better. #LEICHE
Liverpool fans will be encouraged by the credibility of the source. Previously, Alpha Balde was one of the journalists to confirm that Liverpool had bought RB Leipzig midfielder Naby Keita, but would wait to sign the player until the following season.
The Reds may be looking to bolster their midfield options as contract rebel Emre Can still looks likely to leave the club in the summer. Can looked set to join Juventus on a free-transfer at the end of the season, but Real Madrid has apparently become the preferred destination for the German.


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Astrotechnology Brings Nanoparticle Probes Into Sharper Focus
While pondering the challenges of distinguishing one nanosize probe image from another in a mass of hundreds or thousands of nanoprobes, two investigators at Emory University and the Georgia Institute of Technology made an interesting observation. The tiny, clustered dots of light looked a lot like a starry sky on a clear night.
The biomedical researchers realized that astronomers had already made great strides in solving a problem very similar to their own—isolating and analyzing one dot (in this case, a star) in a crowded field of light. They hypothesized that a computer system designed for stellar photometry, a branch of astronomy focused on measuring the brightness of stars, could hold the solution to their problem.
Now, May Wang, Ph.D., at Georgia Tech, and Shuming Nie, Ph.D., and their collaborators have created a technology based on stellar photometry software that provides more precise images of single molecules tagged with nanoprobes, particles specially designed to bind with a certain type of cell or molecule and illuminate when the target is found. The clearer images allow researchers to collect more detailed information about a single molecule, such as how the molecule is binding in a gene sequence, taking scientists a few steps closer to truly personalized and predictive medicine as well as more complex biomolecular structural mapping. In addition to biomedical applications, the system could be used to clarify other types of nanoparticle probes, including tagged particles or molecules. Their research appears in the Proceedings of the National Academy of Sciences of the United States of America (PNAS).
"As more powerful imaging technologies are developed, scientists face a real challenge to quantitatively analyze and interpret these new mountains of data," said Wang. "This paper is only a start, but I expect that innovative computing and data processing will be increasingly used to reveal detailed and quantitative features not currently available to biomedical researchers."
"This work is pointing to a new era in light microscopy in which single-molecule detection is achieved at nanometer resolution," added Nie. "This is also an example of interdisciplinary research in which advanced computing meets nanotechnology. I envision major applications not only for single-molecule imaging but also for ultrasensitive medical diagnostics."
Because scientists frequently use several different colors of nanoprobes to color-code genes and proteins, a blended color dot is a common challenge when analyzing images. For every few green or red dots in an image, there could be a few yellow dots as well, indicating that at least two dots are clustering to create the appearance of a new color. Although less than precise nanoprobe images yield valuable information, the Georgia Tech and Emory research team knew that better technology was needed to pinpoint the exact distance in nanometers between probes to reveal important information about the size and binding geometry of targeted molecules.
"We had no way of knowing for sure if we were looking at one molecule or two or three molecules very near one another," said Wang. "The fuzzy dot images were not precise enough on the nanometer level to truly tell us how these markers reflect DNA, but this system allows us to collect quantitative data and prove—not hypothesize—how genes are behaving."
Instead of starting from scratch to create a system to isolate the clumped nanoprobe images, the Georgia Tech and Emory researchers pursued their stellar photometry idea by adapting DAOPHOT, a program written by Peter Stetson, Ph.D., at the Dominion Astrophysical Observatory, which was designed to handle crowded fields of stars. After adapting DAOPHOT, the research team used color-coded nanoparticles to beat the traditional diffraction limit by nearly two orders of magnitude, allowing routine superresolution imaging at 1-nanometer resolution. And by using DNA molecules, two color-coded nanoparticles are designed to recognize two binding sites on a single target. Then the particles are brought together within nanometer distances after target binding.
These distances are sorted out by highly efficient image processing technology, leading to the detection and identification of individual molecules based on the target's geometric size. Compared with other single-molecule imaging methods, the Georgia Tech and Emory system allows for higher speed detection involving much larger sample volumes (microliter to milliliters).
This work, which was supported in part by the NCI's Alliance for Nanotechnology in Cancer, is detailed in the paper "Nanometer-scale mapping and single-molecule detection with color-coded nanoparticle probes." An investigator from Georgia State University also participated in this study. This paper is available through open access at the journal's Web site (www.pnas.org/cgi/reprint/105/9/3298.pdf).
Source: National Cancer Institute


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Ex-Cowboy Ellis signed to rush QB
Linebacker Greg Ellis has agreed to a three-year deal with the Raiders.
Ellis, 33, was released June 2 by the Dallas Cowboys when the team couldn't find a trade partner.
Ellis, the Cowboys' first-round pick in 1998, has played in 162 games, all with Dallas, starting 156. He has 634 career tackles and 77 sacks, eight last season. He won the NFL Comeback Player of the Year Award in 2007, the same season he was named to the Pro Bowl.
ESPN.com's Bill Williamson was surprised to hear of the signing, because he thought Ellis was bound for Denver or Kansas City.
"Both of those teams are transitioning to a 3-4 defense and are in dire need of a legitimate pass-rusher," Williamson said. "Ellis played in a 3-4 scheme with the Cowboys. ... The Raiders are the only team in the AFC West to use a 4-3 defense."
Ellis will move back to defensive end from outside linebacker.
Oakland has many pass-rushers, including second-year player Trevor Scott, and the Raiders drafted three defensive ends in April.
The signing of Ellis might be another sign that Derrick Burgess could be traded.