text:
Daisy, Holly, Miss Snowflake, Miss April and Miss May here, just!
We went to bed last night at our normal time, got into a comfortable position and drifted off to sleep. Then, just at the right time I woke up and gave Old Two Legs a lick behind his ear as a warning that it was getting close to 'Getting Up Time'.
He takes such a long time to get up that I have to go through this game every morning!
Well, there I was, doing my job, when I happened to look across at the clock.
Three fifty four! That's nearly four in the morning and I always wake up and give him a lick at five!
It was then that I remembered, OTL had said the clocks 'Go Back' tonight, I thought he was sending them all back to the shop!
Well, the upshot of it all was, we got up a whole hour late and it was messing with my body clock!
Even Snowflake was put out and got into her travelling cage before OTL had put her harness on her!
When we got to the Car Park, we met up with Bonnie the Staffy who had been down to the vets for an operation on her claws, the one that you may think of as your thumb.
To stop her from licking the wound she had to wear a horrible plastic collar! I remember when I had to wear one, I hated it!
|That must be horrible to wear!
|'Sorry, can't stop, and Your Late today!'
Having a rummage about on the beach, Holly found a dead crab that had a super sniff about it. As Holly was really getting into the sniff and about to have a lick, OTL spotted her and woofer out for her to leave it alone. Well, actually he woofed 'NO!' Holly was so surprised that she backed off straight away, especially when OTL told her that licking dead crabs would give her the Grumbly Tum!
|It's a Grumbly Tum Crab!
|You've just messed up that sniff!
One of the woofers was fascinated by Snowflake and wouldn't follow her TL, instead she just stood there asking if the ferret tasted like chicken!
|'Come on, tells us, Does it Taste Like Chicken!'
We got back home and after the chores got done OTL set about getting the ceiling sorted out before our midday walk!
It was rather quiet out on the walk, that half a boat was still on the beach although someone had dragged it down to the waters edge, only to be washed further up the beach!
|You still here?
|Sniffing is the best!
Not sure what is for dinner tonight but I reckon there may be some shortbread, I know that 'cos OTL has a brand new bottle of Malt Whisky sitting on the bookcase next to his chair and there is only so much temptation he can take!
Now, Izzy, I've told The Missus to write to your TL about meeting up on the beach, we normally get down there between twelve thirty and one. It will be great fun and we will have the ferrets with us as well!
See you all tomorrow.
Daisy, Holly, Miss Snowflake, Miss April and Miss May.


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Deep business knowledge of Westernacher consultants as a guarantee for managing ERP implementation projects successfully
- Finance & Controlling
Achieving efficient business decision making
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Are you delivering top class customer satisfaction? Pressure to grow the business and minimize the costs while delivery superior customer service has also hit Sales and Service Organizations. Sales and After-Sales Services are of the most critical processes of any company.
SAP Sales and Distribution (SD) and Customer Services (CS) helps to optimize your sales and service processes by automating processes, starting from quotations, sales order, delivery, billing and customer service orders tightly integrated with financial and other logistic components such as PP or MM. Westernacher covers end-to-end business processes and supports you to improve performance and enhance services in order to strengthen customer loyalty.
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Production Planning is an integral part of the logistics function in SAP ERP and is fully integrated with other SAP applications as Materials Management, Plant Maintenance, Sales and Distribution or Financials. Every business that has a manufacturing operation will have implemented some level of production planning functionality to ensure that finished goods can be manufactured at the necessary time for customer's sales orders to be delivered.
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Westernacher supports the seamless link between these activities and functions involved in creating products or services which starts with your suppliers and ends with your customers.

subset: commoncrawl


text:
Hi there,
I just thought I'd pop in for a minute to share some pictures of Lancaster with you.
That's all for now.
it's been awhile, huh? Sorry about the lack of posts lately. What can I say? I actually have a life now…Sort of. 😉 Speaking of which, this weekend one of aunts will be in town and we are running around South Jersey today, having a sleep over (Brendan's coming too!) tonight, and doing the shore tomorrow. I am so excited to do the beach, and in a retro suit no less.It looks something like this, but in chocolate-brown, with a sweetheart neckline:
I am so excited to spend time with my family this weekend. I will be taking a ton of pictures and making up some posts about our time.
Alright everyone, who's with me? Onward to the beach!
Hi guys,
Sorry for my absence lately. Things have gotten really busy around here, and it has been difficult to find the time to get on the interwebs.
As most of you know, Brendan and I spent Mother's Day with my sweet Aunt Cindy, and her family at Longwood Garden, an amazing Eden surrounded by homes and farm land in Pennsylvania.
The whole time we were there I kept on thinking of this Emily Dickinson poem
TWO butterflies went out at noon
Then stepped straight through the firmament,
And rested on a beam; And then together bore away, Upon a shining sea,—
Though never yet, in any port, Their coming mentioned be.
If spoken by the distant bird, If met in ether sea, By frigate or by merchantman, Report was not to me.
We really did have the best time. What did you do with your families yesterday?
That's all for now.
|TWO butterflies went out at noon
|And waltzed above a stream,
|Then stepped straight through the firmament
|And rested on a beam;
|And then together bore away
|Upon a shining sea,—
|Though never yet, in any port,
|Their coming mentioned be.
|If spoken by the distant bird,
|If met in ether sea
|By frigate or by merchantman,
|Report was not to me.
Hi there,
Mother's Day is approaching! What will you be doing for your mothers? This is the first year that I will be spending Mother's Day apart from my mom; and I'll admit that I'm sad. It's hard to spend holidays apart from your family. My wonderful Aunt Cindy knows what it is to be far away from family, having re-located to the east coast about sixteen years ago,so when she invited us to spend Mother's Day with her, I was relieved. Spending time with her, my Uncle Bob, and their boys, makes me feel a lot less homesick.
In honor of the day and my sweet aunt, we will be going to Longwood Gardens.
Doesn't it look beautiful? We are really looking forward to spending the day with my aunt and uncle and their boys. We will be having a picnic lunch in the gardens while we are there, and I want to make something special for everyone.
Here's what I'm thinking:
Flower cupcakes! Appropriate, non? This icing tutorial from Loralee Lewis will be a tremendous help. So I would make a nice vanilla cupcake, with a butter cream frosting…
Or, I could riff on this wonderful Mascarpone-Filled Cake with Sherried Berries recipe, only have mascarpone frosting, with a few dainty berries on the top of the cake…
This is actually my favorite cake to make. It impresses everyone, and it isn't hard to make at all. You should try this! Seriously, what are you waiting for? You will be crowned Cake Queen or King..No, the Mayor of Cake Town… The Ambassador of Delicious Flavor.. I'm finished now, sorry… I tend to get over-excited about pastry.
I hope you are having a glorious Monday.
Yes, apparently while I was in a flu-induces haze, I forgot to post this, even though I had it READY to go for Friday. Blergh.
Happy Friday!
I'm finally feeling a little better! How are you doing? Do you have any big weekend plans?
Yesterday Brendan and I went to Princeton New Jersey (which is such an amazing place). We got this really amazing olive oil and strawberry balsamic at Carter & Cavero: Old World Oil Company, and visited a kitchen gadget store called Kitchen Kapers. It was so much fun being there, in this beyond charming college town, just the two of us focusing on each other. It is wonderful being out together, and to feeling like we did those first few months of being together.
Here we are at Carter & Cavero:
Here I am tasting all of the delicious balsamic vinaigrettes.
There was such a huge selection of oils and vinaigrettes, we had a hard time picking our favorites. In the end we got Truffle Extra-Virgin Olive Oil (so wonderful), Strawberry balsamic, Garlic Extra-Virgin Olive Oil, Tuscan herb Extra-Virgin Olive Oil, and Frantoio Extra-Virgin Olive Oil… Needless to say, we won't be needing EVOO any time soon!
Also, here are somethings that I've been eyeing all week:
These shoes are awesome. I've been wanting to find a pair similar to this for a few years, but nothing really spoke to me like these do.
I love the look of driftwood. Whenever I see a cool drift wood piece it makes me want to learn carpentry so that I could have the ability to make furniture with pieces of awesome wood like the piece above.
Brendan would never let me do this, and I'm not sure I would, but it's pretty cool, right?
Smitten Kitchen's Porch Swing sounds truly sumptuous. Now that the weather is getting warming, I see myself having many a gin based cocktail on balmy New Jersey nights. Yum.
Once again, I pay homage to that glorious hue Kelly Green. This room, designed by Christina Murphy is to die for. Brendan and I both thought this was such a great look. Maybe we will replicate it someday.. : )
And I'll leave you with the sage advice of Ronald Swanson:
Have a stellar weekend! May all your dreams come true.
I'm back, and fully energized (finally)! This last weekend was one of the best I have had in such a long time. Stephanie and I had a great time roaming the streets of New York, and catching up. There is nothing better than sharing a bunch of new experiences with someone you care about.
Here are some pictures from our trip:
And that my readers, is our trip in a nutshell. I loved every minute of it, and wish that we were still there right now. Come back soon Steph!
I have been thinking about things to do when my aforementioned best friend Stephanie comes in to town and here's what I have so far:
1.Christie's: I think it would be so much fun to watch them auction off art and other things. I hear that it is free, and that auctions don't last more than half an hour.
2. Museum of the Moving Image in Queens: Steph is a huge movie buff, I think it would be great to go to a museum that is all about understanding the history of film, the techniques and the technology behind picture shows. 🙂
3. Chelsea Market: I love food, and this looks like the place to go if you like food. I have yet to find many good suggestions of places to eat.. Help!
I would also like to find some good vintage clothing stores to go look at.. Any suggestions?
And that my friends is all I have for now. I have to go finish making that duvet now.
Have any of your heard of Have in Paris? HIP provides short-term vacation rentals in France, England and Tuscany. I discovered this website a couple of months ago and like to visit it occasionally to plan my dream vacation when the weather is particularly dreary out here in NJ. The villas (le sigh) and apartments are so beautiful, and the locations are stellar. I love the idea of being able to wake up in the morning in a little Parisian apartment to a view of Montmartre and Sacre Coeur.
Summer, could you please hurry up and get here?
As I mentioned last week my best friend Stephanie is coming to town at the end of next month, and we are planning on going to New York… So here are some of the things that I'm looking forward to.
The Fashion! I love going too elle.com to look at the Street Chic: New York
There is a kind of quirky chicness about all of these women, that I find absolutely disarming. I'm sure that I will try on everything in my closet and hate it all when we go, but perhaps I'll get some style inspiration after going.
I know it's completely cliched, but I am looking forward to seeing The Empire State Building, is that completely lame? I'm not sure.. But I'll blame An Affair to Remember.
Tres romantique, non? I don't think I actually want to go up, but I do want to see it.
I really want to find The Dessert Truck, any sort of sweet with bacon creme anglaise is good in my book.
And that is all I have for now. Tune in next Thursday for my New York trip plans.
At the end of next month my good friend Stephanie is coming out to Jersey to SEE ME (pause for happy dance)!!! We are planning on going to Philly, since I live so close, and also to New York. I haven't been before (this kills me because we live SO CLOSE), so I don't know what is good to see aside from the obvious things. For those of you who have been there, or live there, what should we do? Where is the good shopping? What about good restaurants?
I hope to hear from you soon..


text: Look for this Quick Ship icon on spec sheets, literature and online product pages for indications as to which items are available in the QS program. A full Quick Ship Guide is being finalized and will be published soon. Please bookmark this page, and check back for it in coming weeks.


text:
The average prices sellers are asking for UK houses are just £30 below their all-time highs and could breach that peak soon as a lack of choice for buyers adds to the upward pressure on values, according to Rightmove.
And as people aged 55 and over prepare to get their hands on their pension pots from next month as a result of new freedoms introduced by the Government, the property website said that a rise in retirees looking to snap up buy-to-let properties could drive house prices higher.
The average new seller asking price across England and Wales was £281,752 in March, which is 1 per cent higher than the previous month and £30 below an all-time high recorded in June last year, Rightmove said.
Bishop's Stortford: 4 High Street, Bishops Stortford, Herts. CM23 2LT• Sales & Lettings: 01279 657 666
Stansted Mountfitchet: 33 Cambridge Road, Stansted, Essex. CM24 8BX• Sales & Lettings: 01279 815 511
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text:
Prayer Performing Live June 2nd Wedding at Gotham
Hall NYC
See us performing live at this fabulous wedding (left). Added onto our
band you can experience the fabulous and famous male vocalist CeCe Rogers
guest starring with our band and also the famous Mitch Matlock vocalist.
We easily can back-up any famous artist that you want to invite to your
event - see how we do it on this video and especially notice how jam packed
the dance floor is the ENTIRE night!!!
Notice that at this wedding we are dressed casually. We can come in tuxedos
and ultra elgantly dressed or we can dress down and do a rock show like
we did at this wedding complete with a sophisticated light show, staging,
dancers, entertainers of all kinds and more. Just ask! No request is too
small or too big for us to handle!
PRAYER - Just the Guys &
See what the boys in our band can do musically and vocally! Between our
cranking horn section and every musician in our band is a star vocalist
as well, each one of us singing better than the next and between us all
we cover so many styles of music it will totally blow your mind! SEE FOR
YOURSELF! Just click PLAY and enjoy! See you at your party!!!
Our gimmick has always been no gimmick - just the music, performed authentically
and true to the original artists, with a high energy presentation. All of
our 8 band members have impressive resumes, and we can add more musicians
(horn players, percussionists, etc.) or any performer you wish to suit your
individual needs and taste.


text:
My Fav Tools
You'll find my favorite business tools for entrepreneurs here including courses, PDFs, blueprints, software, and the latest tech tools that will give you an edge in your business, and simplify your life. (Isn't that what we all want? A little less effort, a little more time, and the capacity to enjoy what we already have right now?). Enjoy.
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Have you been frustrated that your publicity efforts got you little or no publicity? Or you've paid good money to a PR firm, but didn't get the results you'd hoped? Then pay for placement publicity may be for you. You only pay when you're booked on the TV, radio, print, and Internet outlets that YOU choose! Guaranteed. No placements. No payments. Get the gig. Get results. Get happy.
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LeadPages - Opt-ins, Landing pages, Webinars
Use lead pages for automatically formatted and customizable landing pages, opt-in pages, webinar sign up pages, etc. What would take you or your web designer hours takes about 5 minutes in LeadPages. This will save you enormous amounts of time, energy and money and help you streamline your offers. This is what we use for Facebook ads and many of our opt-in pages.
OptimizePress - Landing pages, Sales Pages, Membership Portals
OptimizePress is the new way to create high converting landing pages, sales pages and membership portals. You can use video, audio and text and brand the pages to the look and feel of your own website. We use this in combination with LeadPages as each one has their strengths and is flexible in different areas of design and functionality. Together they create powerful sales funnels so you reach all of the clients and customers who want what you have.
PopupAlly - Polite Popups
An easy to implement popup that can double your conversions…without intruding on your website visitors. No in your face, aggressive hard sell here. You decide how "polite" to be with frequency and placement. Use the #1 proven fastest list-building resource — without being annoying! You'll see these popups on this very site. We hope that intrigue you.
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Want to close every client who is a right for you? Here is the template, the word-for-word script for that [PDF Download]. FYI it's gentle and heart-centered from Sharla Jacobs and Jesse Koren. Thousands of coaches, consultants and healers who couldn't stand to sell have used it successfully. This template gives you the structure of of the conversation to have to close business on the spot — if you are a good match.
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This free blueprint [PDF Download] gives you the big picture on a page and leads you through the system that you can use again and again to fill your client roster with people who are thrilled and eager to work with you.
The Event Filling Blueprint - Use This For Sold Out Workshops, Seminars and Events
Download this free blueprint [PDF Download] for the secrets to pack your own live event to the max from Callan Rush. If you're creating your own workshops, seminars, conferences, retreats or events you'll get the strategies to fill the "room" with the people who are eager to hear from you and buy your products, programs and services. Callan taught her system to thousands of conscious and transformational entrepreneurs including authors, speakers, coaches, consultants, healers, and people in the health and wellness industry.
PRleads - Media Leads
Dan Janal teamed up with Profnet to offer this great new service. Leads are emailed to you almost daily from reporters looking for experts, and you get instant publicity without writing a press release or managing a database. I got an interview with the BBC in less than two weeks, and a colleague of mine was contacted by Business Week magazine the day after he tested this service! Wow. A must.
PitchRate - Free Online PR Training Course Publicity Results
Catapult Your Business With Media Coverage ‐ a two‐week email mini‐course that walks participants through the steps needed to leverage their media coverage to get more clients and build their business through free publicity. You'll also get Free media leads.
HARO - Free Media Leads
From The New York Times, to ABC News, to HuffingtonPost.com and everyone in between, nearly 30,000 members of the media have quoted HARO sources in their stories. Everyone's an expert at something. Sharing your expertise may land you that big media opportunity you've been looking for. You'll get reporters' requests for experts along with story leads sent to your email in‐box daily. Expect leads from every publication from the New York Times to People magazine. Everyday HARO receives hundreds of leads from reporters who are writing articles and desperately need to interview experts like you. If you want to be in the news, HARO is one of the fastest, easiest, and most economical way. Join today. No excuses. It's free.
PRLeads Plus - Guaranteed Press Release Writing and Distribution to Top Media
You'll get your press release written and in front of more than 85,000 journalists and more than 12.3 million people who search Google, Yahoo! and other portals every day. Your press release must appear on at least 40 top‐tier media web sites (where journalists and consumers can find it), with traffic‐boosting links back to your site (so Google can rank it higher).
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You can search this private database of celebrities. Inside you'll find 67,100+ Celebrities, 13,100+ Representatives & 7,100+ Companies. So if you're looking for a celebrity spokesperson, a charity partner, a blurb for your book, this is the place to go.
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Every morning I drink a special cup of Bulletproof coffee that gives you brilliant clarity of mind without the bad buzz. Get ready to learn about how grassfed butter gives you new superpowers (Yup. You put it IN your coffee. Sounds crazy I know). This was a lifesaver for me as peri‐menopausal symptoms such as brain fog, sluggishness and memory loss plagued me. I wasn't even a coffee drinker at all and loved my morning cup of green tea. Harvard trained integrative physician and gynecologist, Dr. Sara Gottfried, (who typically recommends that you get off coffee to help regulate your hormones), suggested I try this regime. Now I'm a total devotee and even bring my own coffee, butter and mct oil (which you'll learn about on the site) when I travel. Plus it tastes delicious. One friend said it was like having a milkshake.
Flexbelt - Use This Quick Toning System - Want to Get Sleek fast for a media appearance?
This is the secret Hollywood stars use to looking great, staying fit and getting a flat stomach fast without exercise. It's super simple. You strap the Flexbelt around you waist and it contracts your muscles like you're doing sit-ups — while you're walking around getting stuff done. I put it on first thing in the morning before I brush my teeth, feed the cats, start my day. I use the butt and thigh belt too and also the flexbelt for the arms.
Also, be sure to check out the micro-stim for the face, too. I use this religiously 5 times a week to keep my completion vibrant and reduce wrinkles. I'm wearing it now!
T‐Tapp - Lose 2 Dress or Pant Sizes in 30 Days
You're a month away from a smaller, more toned you. I went from a size 8 to a size 6 in a month (I'm now a size 4), Lisa Earle McLeod lost 2 dress sizes and got back the flat tummy she hasn't had for 10 years. If you've got a media appearance coming up and you want to get in tip top (T‐Tapp) shape ASAP, click here to order either Total Workout, Basic Plus, T‐Tapp MORE or Total System. (Read the descriptions to find the right program for you.) My sweetie is doing it in the living room right now. Yes, it works for men too. Also check out WhiteBrite, a teeth whitening spray that doesn't make your teeth sensitive and hurt (like all those other whitening kits), so as a TV guest your smile can be as bright as your information.
My Easy Online Store - Shopping Cart
The world's #1 all‐in‐one email ezine service, online shopping cart, auto‐ responder, contact database and online business management system. 1ShoppingCart is the easiest and fastest system to create your very own online store‐front, send out your own professional looking newsletters, have people buy your products and services on the Internet, promote your own teleseminars and webinars and have your very own online business – even if you don't have a website yet.This is what we use. It's an easy reliable system for beginners, intermediate and advanced users.
Pure Chat - Chat live with your website visitors
Use this chat box that you can program to pop up or not, to engage potential customers on your site live or later according to your schedule or the schedule of your customer service team. You'll see it right here on our site. So you can talk to visitors who have questions about your products or services in real-time and get sales on the spot. Use the free version forever if you don't go beyond 15 chats.
Aweber - Newsletter
We use this for our ezine delivery.
Infusionsoft - Shopping Cart
This is the Cadillac of shopping carts that most of the top Internet Marketers use. They have dedicated techs trained on their system to assist you in set up and ongoing support. It's sophisticated – but also complex so plan on spending some time getting to know and understand how it works.
Office AutoPilot - Shopping Cart
People who don't use Infusionsoft but want the same level of sophistication use Office AutoPilot. Most marketers have a dedicated tech running this program as well.
PayPal - Payment System
Over a million websites have set up online payments successfully with PayPal. This is an option inside 1shoppingcart so you don't need to purchase it separately if you buy 1ShoppingCart.
WishList - Easy Membership Software
Turn your blog into a membership site. You can use it as a tool to help you teach courses, as a membership site, for your mastermind group, or a place to collaborate for your subscribers. We use this for our sound bite and mentorship courses as you can program it for different levels of access. A simple, straightforward and inexpensive way to protect your paid content.
Dropbox - Cloud Data Storage
We use this free service to transfer videos and key files, upload Skype & video interviews and files for clients and to access docs when traveling. We couldn't live without it. Sign up for your free account to help you streamline your business.
|*Some of the links above are "affiliate links." This means if you ever purchase a product or service now or in the future offered via my link, I will receive a commission. I only recommend products or services I stand behind, use personally and believe will give you tremendous value. Enjoy!


text:
Publications peter-rugg-gunn
|Title / Authors / Details
|Open Access
Transcription factors make the right contacts.
Nature cell biology, , 1476-4679, , 2019
Author Correction: Promoter interactome of human embryonic stem cell-derived cardiomyocytes connects GWAS regions to cardiac gene networks.
In the original version of the Article, the gene symbol for tissue factor pathway inhibitor was inadvertently given as 'TFP1' instead of 'TFPI'. This has now been corrected in both the PDF and HTML versions of the Article.+ View Abstract
Nature communications, 9, 2041-1723, 4792, 2018
Genome-Scale Oscillations in DNA Methylation during Exit from Pluripotency.
Pluripotency is accompanied by the erasure of parental epigenetic memory, with naïve pluripotent cells exhibiting global DNA hypomethylation both in vitro and in vivo. Exit from pluripotency and priming for differentiation into somatic lineages is associated with genome-wide de novo DNA methylation. We show that during this phase, co-expression of enzymes required for DNA methylation turnover, DNMT3s and TETs, promotes cell-to-cell variability in this epigenetic mark. Using a combination of single-cell sequencing and quantitative biophysical modeling, we show that this variability is associated with coherent, genome-scale oscillations in DNA methylation with an amplitude dependent on CpG density. Analysis of parallel single-cell transcriptional and epigenetic profiling provides evidence for oscillatory dynamics both in vitro and in vivo. These observations provide insights into the emergence of epigenetic heterogeneity during early embryo development, indicating that dynamic changes in DNA methylation might influence early cell fate decisions.+ View Abstract
Cell systems, , 2405-4712, , 2018
Promoter interactome of human embryonic stem cell-derived cardiomyocytes connects GWAS regions to cardiac gene networks.
Long-range chromosomal interactions bring distal regulatory elements and promoters together to regulate gene expression in biological processes. By performing promoter capture Hi-C (PCHi-C) on human embryonic stem cell-derived cardiomyocytes (hESC-CMs), we show that such promoter interactions are a key mechanism by which enhancers contact their target genes after hESC-CM differentiation from hESCs. We also show that the promoter interactome of hESC-CMs is associated with expression quantitative trait loci (eQTLs) in cardiac left ventricular tissue; captures the dynamic process of genome reorganisation after hESC-CM differentiation; overlaps genome-wide association study (GWAS) regions associated with heart rate; and identifies new candidate genes in such regions. These findings indicate that regulatory elements in hESC-CMs identified by our approach control gene expression involved in ventricular conduction and rhythm of the heart. The study of promoter interactions in other hESC-derived cell types may be of utility in functional investigation of GWAS-associated regions.+ View Abstract
Nature communications, 9, 2041-1723, 2526, 2018
Identifying Human Naïve Pluripotent Stem Cells - Evaluating State-Specific Reporter Lines and Cell-Surface Markers.
Recent reports that human pluripotent stem cells can be captured in a spectrum of states with variable properties has prompted a re-evaluation of how pluripotency is acquired and stabilised. The latest additions to the stem cell hierarchy open up opportunities for understanding human development, reprogramming, and cell state transitions more generally. Many of the new cell lines have been collectively termed 'naïve' human pluripotent stem cells to distinguish them from the conventional 'primed' cells. Here, several transcriptional and epigenetic hallmarks of human pluripotent states in the recently described cell lines are reviewed and evaluated. Methods to derive and identify human naïve pluripotent stem cells are also discussed, with a focus on the uses and future developments of state-specific reporter cell lines and cell-surface proteins. Finally, opportunities and uncertainties in naïve stem cell biology are highlighted, and the current limitations of human naïve pluripotent stem cells considered, particularly in the context of differentiation.+ View Abstract
BioEssays : news and reviews in molecular, cellular and developmental biology, , 1521-1878, e1700239, 2018
Long-Range Enhancer Interactions Are Prevalent in Mouse Embryonic Stem Cells and Are Reorganized upon Pluripotent State Transition.
Transcriptional enhancers, including super-enhancers (SEs), form physical interactions with promoters to regulate cell-type-specific gene expression. SEs are characterized by high transcription factor occupancy and large domains of active chromatin, and they are commonly assigned to target promoters using computational predictions. How promoter-SE interactions change upon cell state transitions, and whether transcription factors maintain SE interactions, have not been reported. Here, we used promoter-capture Hi-C to identify promoters that interact with SEs in mouse embryonic stem cells (ESCs). We found that SEs form complex, spatial networks in which individual SEs contact multiple promoters, and a rewiring of promoter-SE interactions occurs between pluripotent states. We also show that long-range promoter-SE interactions are more prevalent in ESCs than in epiblast stem cells (EpiSCs) or Nanog-deficient ESCs. We conclude that SEs form cell-type-specific interaction networks that are partly dependent on core transcription factors, thereby providing insights into the gene regulatory organization of pluripotent cells.+ View Abstract
Cell reports, 22, 2211-1247, 2615-2627, 2018
Molecular profiling of aged neural progenitors identifies Dbx2 as a candidate regulator of age-associated neurogenic decline.
Adult neurogenesis declines with aging due to the depletion and functional impairment of neural stem/progenitor cells (NSPCs). An improved understanding of the underlying mechanisms that drive age-associated neurogenic deficiency could lead to the development of strategies to alleviate cognitive impairment and facilitate neuroregeneration. An essential step towards this aim is to investigate the molecular changes that occur in NSPC aging on a genomewide scale. In this study, we compare the transcriptional, histone methylation and DNA methylation signatures of NSPCs derived from the subventricular zone (SVZ) of young adult (3 months old) and aged (18 months old) mice. Surprisingly, the transcriptional and epigenomic profiles of SVZ-derived NSPCs are largely unchanged in aged cells. Despite the global similarities, we detect robust age-dependent changes at several hundred genes and regulatory elements, thereby identifying putative regulators of neurogenic decline. Within this list, the homeobox gene Dbx2 is upregulated in vitro and in vivo, and its promoter region has altered histone and DNA methylation levels, in aged NSPCs. Using functional in vitro assays, we show that elevated Dbx2 expression in young adult NSPCs promotes age-related phenotypes, including the reduced proliferation of NSPC cultures and the altered transcript levels of age-associated regulators of NSPC proliferation and differentiation. Depleting Dbx2 in aged NSPCs caused the reverse gene expression changes. Taken together, these results provide new insights into the molecular programmes that are affected during mouse NSPC aging, and uncover a new functional role for Dbx2 in promoting age-related neurogenic decline.+ View Abstract
Aging cell, , 1474-9726, , 2018
Naive pluripotent stem cells as a model for studying human developmental epigenomics: opportunities and limitations.
Epigenomics, , 1750-192X, , 2017
Assessing the Safety of Human Pluripotent Stem Cells and Their Derivatives for Clinical Applications.
Pluripotent stem cells may acquire genetic and epigenetic variants during culture following their derivation. At a conference organized by the International Stem Cell Initiative, and held at The Jackson Laboratory, Bar Harbor, Maine, October 2016, participants discussed how the appearance of such variants can be monitored and minimized and, crucially, how their significance for the safety of therapeutic applications of these cells can be assessed. A strong recommendation from the meeting was that an international advisory group should be set up to review the genetic and epigenetic changes observed in human pluripotent stem cell lines and establish a framework for evaluating the risks that they may pose for clinical use.+ View Abstract
Stem cell reports, 9, 2213-6711, 1-4, 2017
Transcriptional response of Hoxb genes to retinoid signalling is regionally restricted along the neural tube rostrocaudal axis.
During vertebrate neural development, positional information is largely specified by extracellular morphogens. Their distribution, however, is very dynamic due to the multiple roles played by the same signals in the developing and adult neural tissue. This suggests that neural progenitors are able to modify their competence to respond to morphogen signalling and autonomously maintain positional identities after their initial specification. In this work, we take advantage of in vitro culture systems of mouse neural stem/progenitor cells (NSPCs) to show that NSPCs isolated from rostral or caudal regions of the mouse neural tube are differentially responsive to retinoic acid (RA), a pivotal morphogen for the specification of posterior neural fates. Hoxb genes are among the best known RA direct targets in the neural tissue, yet we found that RA could promote their transcription only in caudal but not in rostral NSPCs. Correlating with these effects, key RA-responsive regulatory regions in the Hoxb cluster displayed opposite enrichment of activating or repressing histone marks in rostral and caudal NSPCs. Finally, RA was able to strengthen Hoxb chromatin activation in caudal NSPCs, but was ineffective on the repressed Hoxb chromatin of rostral NSPCs. These results suggest that the response of NSPCs to morphogen signalling across the rostrocaudal axis of the neural tube may be gated by the epigenetic configuration of target patterning genes, allowing long-term maintenance of intrinsic positional values in spite of continuously changing extrinsic signals.+ View Abstract
Royal Society open science, 4, , 160913, 2017
Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States.
Human pluripotent stem cells (PSCs) exist in naive and primed states and provide important models to investigate the earliest stages of human development. Naive cells can be obtained through primed-to-naive resetting, but there are no reliable methods to prospectively isolate unmodified naive cells during this process. Here we report comprehensive profiling of cell surface proteins by flow cytometry in naive and primed human PSCs. Several naive-specific, but not primed-specific, proteins were also expressed by pluripotent cells in the human preimplantation embryo. The upregulation of naive-specific cell surface proteins during primed-to-naive resetting enabled the isolation and characterization of live naive cells and intermediate cell populations. This analysis revealed distinct transcriptional and X chromosome inactivation changes associated with the early and late stages of naive cell formation. Thus, identification of state-specific proteins provides a robust set of molecular markers to define the human PSC state and allows new insights into the molecular events leading to naive cell resetting.+ View Abstract
Cell stem cell, , 1875-9777, , 2017
Global reorganisation of cis-regulatory units upon lineage commitment of human embryonic stem cells.
Long-range cis-regulatory elements such as enhancers coordinate cell-specific transcriptional programmes by engaging in DNA looping interactions with target promoters. Deciphering the interplay between the promoter connectivity and activity of cis-regulatory elements during lineage commitment is crucial for understanding developmental transcriptional control. Here, we use Promoter Capture Hi-C to generate a high-resolution atlas of chromosomal interactions involving ~22,000 gene promoters in human pluripotent and lineage-committed cells, identifying putative target genes for known and predicted enhancer elements. We reveal extensive dynamics of cis-regulatory contacts upon lineage commitment, including the acquisition and loss of promoter interactions. This spatial rewiring occurs preferentially with predicted changes in the activity of cis-regulatory elements, and is associated with changes in target gene expression. Our results provide a global and integrated view of promoter interactome dynamics during lineage commitment of human pluripotent cells.+ View Abstract
eLife, 6, 2050-084X, , 2017
DNA methylation is dispensable for changes in global chromatin architecture but required for chromocentre formation in early stem cell differentiation.
Epiblast stem cells (EpiSCs), which are pluripotent cells isolated from early post-implantation mouse embryos (E5.5), show both similarities and differences compared to mouse embryonic stem cells (mESCs), isolated earlier from the inner cell mass (ICM) of the E3.5 embryo. Previously, we have observed that while chromatin is very dispersed in E3.5 ICM, compact chromatin domains and chromocentres appear in E5.5 epiblasts after embryo implantation. Given that the observed chromatin re-organization in E5.5 epiblasts coincides with an increase in DNA methylation, in this study, we aimed to examine the role of DNA methylation in chromatin re-organization during the in vitro conversion of ESCs to EpiSCs. The requirement for DNA methylation was determined by converting both wild-type and DNA methylation-deficient ESCs to EpiSCs, followed by structural analysis with electron spectroscopic imaging (ESI). We show that the chromatin re-organization which occurs in vivo can be re-capitulated in vitro during the ESC to EpiSC conversion. Indeed, after 7 days in EpiSC media, compact chromatin domains begin to appear throughout the nuclear volume, creating a chromatin organization similar to E5 epiblasts and embryo-derived EpiSCs. Our data demonstrate that DNA methylation is dispensable for this global chromatin re-organization but required for the compaction of pericentromeric chromatin into chromocentres.+ View Abstract
Chromosoma, , 1432-0886, , 2017
Derivation and Culture of Epiblast Stem Cell (EpiSC) Lines.
This protocol describes the derivation and culture of epiblast stem cells (EpiSCs) from early postimplantation epiblasts. EpiSCs can be maintained in an undifferentiated state and retain the ability to generate tissues from all three germ layers in vitro and to form teratomas in vivo. However, they seem unable to form chimeras. Whether this is due to differences in developmental status or a cellular incompatibility (e.g., cell adhesion) between EpiSCs and the host inner cell mass (ICM) is currently unclear. Other differences between mouse embryonic stem (ES) cells and EpiSCs also exist, including gene expression profiles and different growth factor requirements for self-renewal. Thus, EpiSCs provide an important in vitro model for studying the establishment and maintenance of pluripotency in postimplantation epiblast tissues.+ View Abstract
Cold Spring Harbor protocols, 2017, 1559-6095, pdb.prot093971, 2017
Derivation and Culture of Extra-Embryonic Endoderm Stem Cell Lines.
Whereas embryonic stem (ES) cells are isolated from the embryonic lineage of the blastocyst, other stable stem cell lines can be derived from the extraembryonic tissues of the early mouse embryo. Trophoblast stem (TS) cells are derived from trophectoderm and early postimplantation trophoblast, and extraembryonic endoderm stem (XEN) cells are derived from primitive endoderm. The derivation of XEN cell lines from 3.5-dpc mouse blastocysts, described here, is similar to the derivation of TS cell lines. TS and XEN cells can self-renew in vitro and differentiate in vitro and in chimeras (in vivo) in a lineage-appropriate manner, showing the developmental potential of their origin, thus providing important models to study the mouse extraembryonic lineages.+ View Abstract
Cold Spring Harbor protocols, 2017, 1559-6095, pdb.prot093963, 2017
XACT Noncoding RNA Competes with XIST in the Control of X Chromosome Activity during Human Early Development.
Sex chromosome dosage compensation is essential in most metazoans, but the developmental timing and underlying mechanisms vary significantly, even among placental mammals. Here we identify human-specific mechanisms regulating X chromosome activity in early embryonic development. Single-cell RNA sequencing and imaging revealed co-activation and accumulation of the long noncoding RNAs (lncRNAs) XACT and XIST on active X chromosomes in both early human pre-implantation embryos and naive human embryonic stem cells. In these contexts, the XIST RNA adopts an unusual, highly dispersed organization, which may explain why it does not trigger X chromosome inactivation at this stage. Functional studies in transgenic mouse cells show that XACT influences XIST accumulation in cis. Our findings therefore suggest a mechanism involving antagonistic activity of XIST and XACT in controlling X chromosome activity in early human embryos, and they highlight the contribution of rapidly evolving lncRNAs to species-specific developmental mechanisms.+ View Abstract
Cell stem cell, , 1875-9777, , 2016
Deletion of the Polycomb-Group Protein EZH2 Leads to Compromised Self-Renewal and Differentiation Defects in Human Embryonic Stem Cells.
Through the histone methyltransferase EZH2, the Polycomb complex PRC2 mediates H3K27me3 and is associated with transcriptional repression. PRC2 regulates cell-fate decisions in model organisms; however, its role in regulating cell differentiation during human embryogenesis is unknown. Here, we report the characterization of EZH2-deficient human embryonic stem cells (hESCs). H3K27me3 was lost upon EZH2 deletion, identifying an essential requirement for EZH2 in methylating H3K27 in hESCs, in contrast to its non-essential role in mouse ESCs. Developmental regulators were derepressed in EZH2-deficient hESCs, and single-cell analysis revealed an unexpected acquisition of lineage-restricted transcriptional programs. EZH2-deficient hESCs show strongly reduced self-renewal and proliferation, thereby identifying a more severe phenotype compared to mouse ESCs. EZH2-deficient hESCs can initiate differentiation toward developmental lineages; however, they cannot fully differentiate into mature specialized tissues. Thus, EZH2 is required for stable ESC self-renewal, regulation of transcriptional programs, and for late-stage differentiation in this model of early human development.+ View Abstract
Cell reports, 17, 2211-1247, 2700-2714, 2016
Crosstalk between pluripotency factors and higher-order chromatin organization.
Pluripotent cells are characterized by a globally open and accessible chromatin organization that is thought to contribute to cellular plasticity and developmental decision-making. We recently identified the pluripotency factor Nanog as a key regulator of this form of chromatin architecture in mouse embryonic stem cells. In particular, we demonstrated that the transcription factors Nanog and Sall1 co-dependently mediate the epigenetic state of pericentromeric heterochromatin to reinforce a more open and accessible organization in pluripotent cells. Here, we summarize our main findings and place the work into a broader context. We explore how heterochromatin domains could be targets of transcriptional networks in pluripotent cells and are coordinated with cell state. We propose this integration may be to balance the requirement for a dynamic and plastic chromatin organization in pluripotent cells, together with priming for a more restrictive nuclear compartmentalization that is triggered rapidly upon lineage commitment.+ View Abstract
Nucleus (Austin, Tex.), , 1949-1042, 0, 2016
Comparative Principles of DNA Methylation Reprogramming during Human and Mouse In Vitro Primordial Germ Cell Specification.
Primordial germ cell (PGC) development is characterized by global epigenetic remodeling, which resets genomic potential and establishes an epigenetic ground state. Here we recapitulate PGC specification in vitro from naive embryonic stem cells and characterize the early events of epigenetic reprogramming during the formation of the human and mouse germline. Following rapid de novo DNA methylation during priming to epiblast-like cells, methylation is globally erased in PGC-like cells. Repressive chromatin marks (H3K9me2/3) and transposable elements are enriched at demethylation-resistant regions, while active chromatin marks (H3K4me3 or H3K27ac) are more prominent at regions that demethylate faster. The dynamics of specification and epigenetic reprogramming show species-specific differences, in particular markedly slower reprogramming kinetics in the human germline. Differences in developmental kinetics may be explained by differential regulation of epigenetic modifiers. Our work establishes a robust and faithful experimental system of the early events of epigenetic reprogramming and regulation in the germline.+ View Abstract
Developmental cell, 39, 1878-1551, 104-115, 2016
The pluripotency factor Nanog regulates pericentromeric heterochromatin organization in mouse embryonic stem cells.
An open and decondensed chromatin organization is a defining property of pluripotency. Several epigenetic regulators have been implicated in maintaining an open chromatin organization, but how these processes are connected to the pluripotency network is unknown. Here, we identified a new role for the transcription factor NANOG as a key regulator connecting the pluripotency network with constitutive heterochromatin organization in mouse embryonic stem cells. Deletion of Nanog leads to chromatin compaction and the remodeling of heterochromatin domains. Forced expression of NANOG in epiblast stem cells is sufficient to decompact chromatin. NANOG associates with satellite repeats within heterochromatin domains, contributing to an architecture characterized by highly dispersed chromatin fibers, low levels of H3K9me3, and high major satellite transcription, and the strong transactivation domain of NANOG is required for this organization. The heterochromatin-associated protein SALL1 is a direct cofactor for NANOG, and loss of Sall1 recapitulates the Nanog-null phenotype, but the loss of Sall1 can be circumvented through direct recruitment of the NANOG transactivation domain to major satellites. These results establish a direct connection between the pluripotency network and chromatin organization and emphasize that maintaining an open heterochromatin architecture is a highly regulated process in embryonic stem cells.+ View Abstract
Genes & development, , 1549-5477, , 2016
Chromatin organization in pluripotent cells: emerging approaches to study and disrupt function.
Translating the vast amounts of genomic and epigenomic information accumulated on the linear genome into three-dimensional models of nuclear organization is a current major challenge. In response to this challenge, recent technological innovations based on chromosome conformation capture methods in combination with increasingly powerful functional approaches have revealed exciting insights into key aspects of genome regulation. These findings have led to an emerging model where the genome is folded and compartmentalized into highly conserved topological domains that are further divided into functional subdomains containing physical loops that bring cis-regulatory elements to close proximity. Targeted functional experiments, largely based on designable DNA-binding proteins, have begun to define the major architectural proteins required to establish and maintain appropriate genome regulation. Here, we focus on the accessible and well-characterized system of pluripotent cells to review the functional role of chromatin organization in regulating pluripotency, differentiation and reprogramming.+ View Abstract
Briefings in functional genomics, , 2041-2657, , 2015
Annual meeting of the EpiGeneSys Network of Excellence - Advancing epigenetics towards systems biology.
The third annual meeting of the EpiGeneSys network brought together epigenetics and systems biologists to report on collaborative projects that apply quantitative approaches to understanding complex epigenetic processes. The figure shown represents one meeting highlight, which was the unexpected emergence of genotype versus epigenotype in control of cell state.+ View Abstract
BioEssays, 2015, , , , 2015
Epigenetic features of the mouse trophoblast.
Trophoblast cells are required for the growth and survival of the fetus during pregnancy, and failure to maintain appropriate trophoblast regulation is associated with placental insufficiencies and intrauterine growth restriction. Development of the trophoblast lineage is mediated by interactions between genetic and epigenetic factors. This review will focus on new insights that have been gained from analysis of mouse models into the epigenetic mechanisms that are required for the early establishment of the trophoblast lineage and for the development of specialized cell types of the fetal placenta. In particular, the importance of DNA methylation, 5-hydroxymethylcytosine and histone modifications in orchestrating trophoblast gene expression and functional outcome will be discussed. These insights are beginning to be extended towards human studies and initial results suggest that the causes and consequences of a variety of placental pathologies are related to epigenetic processes. Furthermore, the epigenetic landscape that regulates trophoblast cells seems to be particularly vulnerable to perturbation during development. This has major implications for diet and other environmental factors during pregnancy.+ View Abstract
Reproductive biomedicine online, 25, 1, 21-30, 2012
Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells.
The advent of reprogramming and its impact on stem cell biology has renewed interest in lineage restriction in mammalian embryos, the source of embryonic (ES), epiblast (EpiSC), trophoblast (TS), and extraembryonic endoderm (XEN) stem cell lineages. Isolation of specific cell types during stem cell differentiation and reprogramming, and also directly from embryos, is a major technical challenge because few cell-surface proteins are known that can distinguish each cell type. We provide a large-scale proteomic resource of cell-surface proteins for the four embryo-derived stem cell lines. We validated 27 antibodies against lineage-specific cell-surface markers, which enabled investigation of specific cell populations during ES-EpiSC reprogramming and ES-to-XEN differentiation. Identified markers also allowed prospective isolation and characterization of viable lineage progenitors from blastocysts by flow cytometry. These results provide a comprehensive stem cell proteomic resource and enable new approaches to interrogate the mechanisms that regulate cell fate specification.+ View Abstract
Developmental cell, 22, 4, 887-901, 2012
Global chromatin architecture reflects pluripotency and lineage commitment in the early mouse embryo.
An open chromatin architecture devoid of compact chromatin is thought to be associated with pluripotency in embryonic stem cells. Establishing this distinct epigenetic state may also be required for somatic cell reprogramming. However, there has been little direct examination of global structural domains of chromatin during the founding and loss of pluripotency that occurs in preimplantation mouse development. Here, we used electron spectroscopic imaging to examine large-scale chromatin structural changes during the transition from one-cell to early postimplantation stage embryos. In one-cell embryos chromatin was extensively dispersed with no noticeable accumulation at the nuclear envelope. Major changes were observed from one-cell to two-cell stage embryos, where chromatin became confined to discrete blocks of compaction and with an increased concentration at the nuclear envelope. In eight-cell embryos and pluripotent epiblast cells, chromatin was primarily distributed as an extended meshwork of uncompacted fibres and was indistinguishable from chromatin organization in embryonic stem cells. In contrast, lineage-committed trophectoderm and primitive endoderm cells, and the stem cell lines derived from these tissues, displayed higher levels of chromatin compaction, suggesting an association between developmental potential and chromatin organisation. We examined this association in vivo and found that deletion of Oct4, a factor required for pluripotency, caused the formation of large blocks of compact chromatin in putative epiblast cells. Together, these studies show that an open chromatin architecture is established in the embryonic lineages during development and is sufficient to distinguish pluripotent cells from tissue-restricted progenitor cells.+ View Abstract
PloS one, 5, 5, e10531, 2010


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MANKATO, Minn. (Blugolds.com) – The UW-Eau Claire women's basketball team fell short 59-50 to NCAA Division II opponent Minnesota State University-Mankato tonight.
The Blugolds led at two different times in the first minute but after Eau Claire tied it up at 8-8 four minutes into the game, Mankato took a lead on its next possession and never looked back.
The first half remained close, however, as the Blugolds stayed within seven points the entire time. At one time, the Blugolds used a 5-0 run to close to within one point and found themselves down by just two points with less than two minutes remaining in the half. But MSU closed the half on a 5-0 run to take a seven-point lead into halftime.
In the second half, Mankato opened on a 10-3 run to get up by 14 points, and while the Blugolds were able to get within five a few times the rest of the way, MSU hung on to remain undefeated on the season.
Nicole Christianson (Sr.-Chetek, Wis.) led the Blugolds in scoring and rebounding with 19 points and seven rebounds. Ellen Plendl (Sr.-Chippewa Falls, Wis./McDonell) added 13 points and had five rebounds. While Sarah Bingea (Jr.-Delano, Minn.) had nine points, she was just 1-for-11 from the field. Seven of her points came at the free throw line, where she was 7-for-8. As a team, the Blugolds were 13-for-16 from the line (81.3 percent).
Eau Claire drops to 3-2 on the season while Mankato improves to 4-0. The two teams have only met one other time in history, a season ago, and the Blugolds won that game 66-54.
Sunday the Blugolds will be home for a contest against Cornell College (Iowa). Action is set to get underway at 3 p.m.


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The FedGlobal EconomyMoney and Banking
The true lesson from Japan is that central planners prefer to gradually nationalize the economy before even considering a moderate reduction in government size and control.
British politics could potentially be very different next year, but a recent shortlist of candidates to replace Mark Carney doesn't provide much hope for Austrians.
Free MarketsGlobal EconomyProtectionism and Free Trade
Trade agreements have thus become obsolete tokens of negotiation in larger geopolitical disputes, protectionist tools for managing and interfering with global trade flows.
Had consumers in 1938 been uninterested in a feature-length cartoon, Snow White would have bankrupted the Disney studio. Instead, Walt Disney was richly rewarded for his risk-taking.
Free MarketsU.S. EconomyInterventionism
This immense cooperative system is known as a free-market economy. It was not consciously planned by anybody. It evolved.
China is not on track to catch up with the wealthy West. The Chinese state's commitment to highly interventionist mercantilist policies is leading to debt, malinvestment, and stagnation.
Political and economic salvation for Venezuela can come only from the Venezuelan people themselves. They cannot be "forced to be free."
Decentralization and Secession
So long as we insist the federal government can force one law, one culture, and one sort of politics on all of America, the US is headed down the road of civil war. Only decentralization and separation can partly defuse the situation.


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Pinion seals
SKF pinion seals are built to meet or exceed OE specifications. The complete line of SKF pinion seals includes direct replacement designs, double lip (DL) designs and unitized designs.
The DL pinion seal, made from a proven fluoroelastomer that's been enhanced for improved sealing performance, features a patented Waveseal® bi-rotational design that dissipates heat and reduces wear. A rugged excluder lip and a radial dirt lip offer two barriers against dirt and contamination, while a Bore-Tite® sealant on the outer diameter eliminates oil leaks caused by scratches or minor imperfections in the bore.
The unitized pinion seals offer the optimum sealing performance with extended life. The superior design provides a perfectly matched running surface for the sealing lips made from highly engineered fluoroelastomer. High temperature capable with extensive exclusion properties provides long life in a highly aggressive application. The unitized design incorporates the patented wave seal that sweeps lubricant back to the bearings promoting cooler running operation. Another innovative feature of this seal adding long life is the 1/2 metal, 1/2 rubber ID allows for heat dissipation while holding a positive grip on the shaft.


text: Edible Glue Edible Glue Edible Glue (8 ratings) Edible Glue by Beau Products £2.75 We're more than happy to let you know when we expect more stock. Please email us. Edible Glue for use with the Flower Paste by Beau Products and other florist paste/sugarpaste. Shake the bottle gently before opening. Use sparingly, the glue likes to be left for a few seconds before building up and layering your project. It becomes nice & tacky when used, rather than wet. Does not have an "acidy" smell to it like other Edible Glue and this is now the only glue we recommend. Ingredients - Bovine Gelatine, Vegetable Fat, Glucose, Icing sugar, water, Gum E413, Meri white preservative


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With 2013 recording one of the coldest springs for 50 odd years, our British Butterflies have suffered a further set-back. Many have been late to emerge and our rarer species are now on the wing anything up to several weeks later than would be expected.
Butterfly Conservation have stated in an article I read recently, that the Pearl-bordered Fritillary did not emerge until April 27th (usually this species is on the wing by April 1st). The rare and endangered Wood White was first seen in early May (On the wing in 2012 by April 10th) and the Duke of Burgundy was only on the wing in late April, (up to three weeks later than 2012).
Grizzled Skipper
Pearl-bordered Fritillary
Duke of Burgundy
Wood White
2012 was a complete washout year and one of the worst years on record for our British Butterflies. Many species are in decline. How will they fare this year with such a cold spring? Lets hope that the weather improves and they have a successful breeding season, in order for their ever dwindling numbers to show some signs of increase.
High Brown Fritillary
During 2012 the critically endangered High Brown Fritillary saw its population drop by 46% and the Marsh Fritillary by 71%. Populations of many butterflies and moths are depressing and species that were once common during the last decade or so are now showing dramatic reductions in their range & numbers. I can't bear the thought of not seeing a Small Tortoiseshell grace our British Countryside.
For further reading see – Butterfly Conservation's publication "The State of Britain's Larger Moths 2013" and "The State of Butterflies in Britain and Ireland".
Small Tortoiseshell


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Transparent cases seemed to be all the rage a few years ago, but I never invested in one mainly because they carried a premium price. They also only looked great if you added a bunch of lights inside, and even then you'd probably end up hiding the case under your desk.
Now Japanese manufacturer Scythe is launching a rather unusual new transparent case that has an additional feature: it's an open case. What that means is there are no side panels to encase the components. Instead, everything is left open to the air (and dust) around them.
The Scythe ACB-TYPE3 may seem like a mistake from a dust point of view, but it could be the ideal case for someone who regularly tests different components and wants quick access to them. It also opens up the cooling options you have somewhat. For example, you could mount as big a fan as you want on the side of this case, or even completely independently of it.
While open, it's certainly not small and has space for three 5-inch drives, three 3.5″ drives, and two 2.5″ drives. It will work with ATX, micro-ATX, and mini-ITX boards and there's no chance of you breaking it due to the use of 5mm thick acrylic. In terms of weight, there's definitely a saving due to it being an open case. As a comparison, the fully-enclosed transparent Scythe ACC-ATX case weighs 5.2kg. The ACB-TYPE3 is only 3.4kg.
For the moment the ACB-TYPE3 is only appearing on the Japanese Scythe website, but it's likely a number of component stores will order stock. It's certainly a different take on a PC case and one some rig builders are sure to want to experiment with, especially as the price tag is only $85.
Read more at Scythe, via FanlessTech


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I was so happy yesterday to head over to the post office and discover a package from my friends at Wisconsin Public Radio (wpr.org). My water bottle (and Francie's tote bag) had arrived! Time to get on top of hydration. I can't tell you how many times 6pm has rolled around, and I realized I haven't had a drop of water all god-damned day! Definitely some coffee, probably some beer, but not honest to goodness liquid water. You know – the building block of life?
Anyways, I'm loving my new little water bottle, and if you find yourself in the same position I did every day, maybe it's time to grab one of your own. We'll see if I keep up with the hydration once the novelty of mine wears off. I'll cross my fingers.
Call To Action – Get up right now and have a sip of water. Your body will thank you for it. While you're at it, why not throw some cash at your local public radio station? Your community will thank you for it. You can find your local station here.


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China's Hanification of East Turkestan: Failing? -By Habib Siddiqui
On Saturday, Mar. 1, more than 10 assailants slashed scores of people with knives at the Kunming train station in Yunnan province in southern China in what state media said … Continue reading
Sri Lanka: Muslims suffer amid Tiger-army fighting
By Fahad Ansari Crescent MaY 2009 This month, the world may witness the final chapter in the 25-year-old conflict between the Sri Lankan army and the Liberation Tigers of Tamil … Continue reading
Uighur Muslims Condemn China Knife Attack
Uighur Muslims have vehemently condemned Saturday's attacks on a Chinese train station which left dozens killed and injured, amid accusations that point fingers at the persecuted Uighur Muslims in … Continue reading
Aussies Buy Indonesia's Halal Certificates
Indonesia's top Muslim body has come under criticism after facing accusations of getting bribes from Australian halal certification companies, a charge vehemently denied by Indonesian scholars. "It's common knowledge in … Continue reading
Ethnic Profiling, Nigeria's Looming Crisis
Concerned by the recent arrests targeting northern Nigerians, analysts and Muslim leaders have warned that the security agencies moves in Nigeria's south were threatening radicalizing more northern young Muslims who … Continue reading
Uganda Muslims Laud Anti-Gay Bill
Uganda Muslims have welcomed the decision of President Yoweri Museveni to back an anti-gay bill that imposes life sentences for some homosexual acts, risking ties between western donors and … Continue reading
Filipino Poor Muslims Aid Typhoon Survivors
Defying their poverty and dire need, Muslims in Marawi City in Mindanao have been campaigning to raise funds and aid to the Yolanda typhoon stricken areas in the Visayas. It … Continue reading
Angola Bans Islam, Destroys Mosques
According to several Angolan newspapers, Angola has become the first country in the world to ban Islam and Muslims, taking first measures by destroying mosques in the country. "The process … Continue reading
India's Narendra Modi uses a rich heritage as political camouflage
AHMEDABAD, India — When the renowned French architect Le Corbusier came to this city in western India in 1951, he was so awestruck by the Sarkhej Roza, an Islamic monument on … Continue reading
"Democratization in Myanmar hampered by anti-Muslim discrimination"
JTW Interview, Prof. Dr. Selçuk Çolakoğlu Dr. Selçuk Çolakoğlu, Director of USAK Center for Asia-Pacific Studies, sat down for an interview about the grave conditions surrounding the Muslim population in … Continue reading
Myanmar's infant mortality woes
MAE SOT (NORTHWESTERN THAILAND), 15 November 2013 (IRIN) – A donor-funded clinic in northwestern Thailand is helping save the lives of underweight babies born to impoverished Burmese mothers who have … Continue reading
Police cruelly beaten up an 8 years old child in oo shin kya village Under Maung Daw
A youth from Oo shin kya village report that the police group about whom I mentioned in my earlier report "police harassment in Maung daw northern Aarkan", was mercilessly beaten … Continue reading
Turkey Allows Hijab In Bar Association
Marking another victory for hijab in Turkey, a supreme court has reversed a headscarf ban for female lawyers, granting them the right to register at the Turkey Bar Association (TBB) … Continue reading
Refugees in Bulgaria face death threats
This is not the safe heaven Ali was expecting. Chased from his hometown of Aleppo by the horrors of the Syrian civil war, he and his extended family of 17 … Continue reading
The Arab Influence on the Italian Renaissance
Beetle Juice, anyone? Since much of it happened by way of southern Italy, I think I can justify sneaking it in, here. This is an early-15th-century Persian copy of the … Continue reading
Martyrdom bombing kills and wounds over 40 Russians in Muslim Idel Ural
The bombing took place in the Russian-occupied Muslim city of Sari Chin (Russian name, Volgograd, or Stalingrad) in Idel Ural, which was officially recognized as an oppressed independent country in a US … Continue reading
Muslim pupils, 14, banned from the classroom at Catholic school for refusing to shave off their beards after claiming their religion forbids it
Students have been in 'isolation' at Lancashire school for almost a month The school has said the pupils, both 14, are breaching the dress code Families say the boys are … Continue reading
Quran Translation Banned By Russian Court; Top Islamic Cleric Ravil Gainutdin Protests 'Outrageous' Order
MOSCOW — Russia's top Islamic cleric has protested a provincial court order to declare a translation of the Quran as extremist and to destroy it. Ravil Gainutdin, the head of … Continue reading
Life and death in Aleppo (PHOTOS)
Friday, September 7th, 2012 ALEPPO, Syria — Earlier this week, I was filming a feature on life on the frontlines of Aleppo, Syria. I was camping out with the men … Continue reading
#SaveMaryam Campaign | Why ?
Up to 2 million people are walking away from Islam in Indonesia every year. Detachment and disillusionment has left them feeling that Islam is out of touch with the lives … Continue reading
Muslims Killing in Burma and Social Media Manipulating Images
In now a days people are posting pictures with captions like Muslims killing in Burma, Muslims slaughter by Buddhists in Burma.. I have search reality of few pics and found following: A picture shared … Continue reading
Massacres of Arakan's Muslims between the reality and the imagination .. the full report !
What a misery and what a suffering and what a tragedy suffered by Muslims in Burma ..?! what a war and what a genocide practiced against our Muslim there ..?! … Continue reading
More than 200 Asylum-Seeking Uyghurs Detained in Thailand
More than 200 Uyghurs are being held at an immigration center near Thailand's southern city of Hat Yai's international airport after they were detained in a secret camp in a … Continue reading
Chicago Muslims Welcome Arrests of Vandal
A leading Chicago Muslim organization has welcomed the arrest of a woman who vandalized 21 houses of worship throughout Lake County during the span of one week, the attacks which … Continue reading


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Sometime, it is better to have simply heard a song than to have seen it and had your vision of it ruined.
हर कोई चाहता है एक मुट्ठी आसमान ...
Today, I had the misfortune of having this Kishor Kumar gem ruined by its picturization. Oh dear! I wish I could go back 5 minutes in time...
Thanks for wandering in. Join me as I jaywalk through the thoughts of columnists, sports figures and sometimes mine.
Tuesday, May 18, 2010
Better left unseen
Posted by Jaunty Quicksand at 2:03 PM No comments:
Labels: Entertainment, Songs, Videos
Monday, May 17, 2010
Maggie Noodle Review - Iron Man 2
Iron Man 2 picks up from a little after the first Iron Man movie ended. The first movie took its time getting into what makes Tony Stark tick (almost literally) and this movie takes the premise even further. Robert Downey, Jr., plays Stark, the man behind the outfit, as a fellow who laps up the attention and uses Iron Man's visibility as a deterrent to the bad guys. He has an ulterior motive for exposing a narcissistic streak but just as the movie gets interesting a curious thing happens. The attention is repeatedly taken away from RDJ and shifted elsewhere.
Posted by Jaunty Quicksand at 10:54 AM No comments:
Labels: Entertainment, Maggi Noodle Reviews, Movie Reviews, Movies, Thoughts
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All posts by admin
Accounts Manager or Accounts Executive Job in Pasir Gudang Johor
Job Vacancies in February 2014
We have a senior position in our ACCOUNTS DEPARTMENT to be filled by an ACCOUNTS MANAGER, ASSISTANT ACCOUNTS MANAGER OR ACCOUNTS EXECUTIVE, who will be reporting directly to our Top Management. Candidate is expected to prepare monthly financial analysis reports for management review and advice management on all financial related matters.
If interested, please call Andrew ( 012 7189 700).
Wishing you a Happy Chinese New Year of the Horse
Scheduled Waste Processor for Additional Codes
Happy & Healthy Greetings!
CHEMALAYA has been recently licensed by Jabatan Alam Sekitar Negeri Johor (Department of Environment) to process an additional 11 codes:-
1) SW 104 – ARP Dust, Zinc Dross & Aluminium Dross
2) SW 110 – Stamping Metal with Coating
3) SW 204 – Waste water Treatment Plant Sludge with Metal content
4) SW 305 – Spent Lubricant Oil
5) SW 306 – Spent Hydraulic Oil
6) SW 307 – Spent Mineral Oil-Water Emulsion
7) SW 311 – Oil Wastes or Oily Sludge
8) SW 312 – Oil Residue from workshops, kiosks and grease traps
9) SW 314 – Oil or Oil Sludge from oil refineries and petrochemical plants
10) SW 409 – Used Metal Drums, Plastic Drums, Barrels, IBC's and Jerry cans
11) SW 410 – Kraft Paper contaminated with oil from steel roll industry
Towards a Cleaner tomorrow, 🙂
Implementation of New Scheduled Waste Codes
In 2012, Chemalaya has started upgrading our facilities to process further Scheduled Waste Codes. We are currently working towards licensing to collect and process the following waste codes in early 2013:-
1) SW 410 – Kraft paper contaminated with oil from steel roll industry
2) SW 409 – Used metal drums
3) SW 305 – Spent lubricant oil
4) SW 306 – Spent hydraulic oil
5) SW 307 – Spent Mineral oil (water emulsion)
6) SW 204 – Sludge
7) SW 104 – ARP Dust
8) SW 110 – Stamping metals with coating
Cultivating an environmentally conscious culture in Johor, 🙂
Scheduled Waste Contractor for SW 422
"We are a DOE approved Scheduled Waste and Non-Scheduled Waste Processor specializing in handling the following:-"
Mixture of Scheduled and Non-Scheduled Waste
- Aluminium Waste Contaminated With Scheduled Waste (Waste Code: SW 422)
- Metal/Iron/Stainless Steel/Nickel Waste Contaminated With Scheduled Waste (Waste Code: SW 422)
- Copper/Bronze/Brass Waste Contaminated With Scheduled Waste (Waste Code: SW 422)
Our website is currently under construction!
Kindly call us or drop us an email if you have any inquiries.
Lot 128, Jalan Rumbia 3
Tanjung Langsat Industrial Complex
81700 Pasir Gudang
Johor, MALAYSIA
Telephone Number : +607 255 2133
Facsimile Number : +607 255 3133
Email: firstname.lastname@example.com


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On December 5th, the Anti-Infective Drugs Advisory Committee (AIDAC) discussed new drug application (NDA) 206494 for ceftazidime-avibactam for injection, submitted by Cerexa Inc. (a subsidiary of Actavis), for the proposed indications of: Complicated Intra-abdominal Infections (cIAI), Complicated Urinary Tract Infections (cUTI), including Acute Pyelonephritis and Limited Use Indication: Aerobic Gram-negative Infections with Limited Treatment Options.
Ceftazidime-avibactam is an investigational antibiotic being developed to treat serious Gram-negative bacterial infections in hospitalized patients when limited or not treatment options are available. It consists of ceftazidime, a third-generation, antipseudomonal cephalosporin that is a well-established treatment for serious Gram-negative bacterial infections, and avibactam, a next generation, non-β lactam β-lactamase inhibitor.
Ceftazidime-avibactam inhibits a broad range of β- lactamases, including Class A (Extended-Spectrum β-Lactamases and Klebsiella pneumoniae carbapenemase), Class C (AmpC) and some class D enzymes produced by Gram-negative pathogens that are encountered in the hospital setting and involved in some of the most serious infections such as cIAI and cUTI.
The panel was asked four voting questions regarding the efficacy and safety of ceftazidime-avibactam, and found the scientific and clinical evidence submitted by Cerexa adequately demonstrated the safety and efficacy of ceftazidime-avibactam in the treatment of the proposed cIAI and cUTI indications. As such, panel members voted in favor of approval.
Panelists, however, raised concerns around labeling, particularly for patients with renal impairment. The committee voted not to recommend approval of the combination for hospital-acquired bacterial pneumonia (HABP)/ventilator-associated bacterial pneumonia (VABP) and bacteremia.
"We are pleased with the committee's positive recommendation for ceftazidime-avibactam. It reinforces our belief in the safety and efficacy of the compound and its potential to address significant unmet needs as a new treatment option for patients with serious and life-threatening infections," said David Nicholson, senior vice president, Global Brands R&D, Actavis.
Actavis expects FDA action on its NDA for ceftazidime-avibactam during the first quarter of 2015. The FDA is not obligated to follow the advice of its panels, but normally does so.
ISS has over a decade of experience in developing regulatory strategies including support for FDA Advisory Committee meetings. We are involved in more FDA AdComs per year than even the largest pharmaceutical companies. For more information on how ISS can help you prepare for your next meeting, contact email@example.com.
Do you have an upcoming Advisory Committee Meeting?


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Loot Crate March 2015 Spoilers & Coupon
The March 2015 Loot Crate will be all about covert operations! (Thanks for the heads up on the spoiler Lily!)
The box will include "an EXCLUSIVE, officially licensed Marvel's Agents of S.H.I.E.L.D. replica collectible (complete with certificate of authenticity!); plus a Loot Crate Exclusive Orphan Black item; the return of an EXCLUSIVE t-shirt (not black!), plus some incredibly cool undercover-rrific wearable accessories; and much more!"
Here are the details:
If you haven't signed up yet, use coupon code SAVE3 to save $3 off your firstLoot Crate box!
What do you think of the spoilers?


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Hi there!
My name is Bethany Mortelliti and I'm the girl behind St. Bosco Edits.
My professional mission is to help non-profits, churches, and students produce meaningful, coherent, and clear communication pieces.
I'd love to get in touch with you today to learn more about what you're looking for. Get in touch today!