text:
I don't mean to mean to be callus. Truly, many of these building are stunning and should most definitely be appreciated. But, in a country a millennium older than my own, I wanted to touch buildings and statues and non-man-made objects that were ORIGINAL, that had stood the test of time. My first couple of years here, I was disappointed. So many of the touristy things had been rebuilt due to fire, earthquakes, wars, and, um, bombs. But once I chatted with some of my travel-experienced friends and did some research, I realized that there are a lot of several-centuries-old things still standing. It's just most of them are off the "beaten path."
So, when I visit my first original Japanese castle, it was as a side trip to the main event we road tripped to: the annual fertility festival near Nagoya held March 15. I had been in 2014 with the spouse, but this spring, I went with two members of my "lady clique" and a baby. Yes... I realize that taking a baby to a fertility festival is just unnecessary... obviously someone is fertile. But the fertile one happened to provide sustenance for her child, so said child was packed along. You can see he had a lot of fun.
Of course, we did family-friendly activities, too... I visited Nagoya Castle for the second time. It has been rebuilt from being destroyed by air raids in 1945, and isn't original, but it is still beautiful.
But, of course we had to get to the fertility festival, which kicks of in the mid-afternoon with a parade from one shrine to another.
Once we had our fill of wooden phalluses, we took some advice from our friend Carolyn and drove about 20 minutes to Inuyama Castle... the first that I saw of the original National Treasure Castles. We checked out the shrine below it first, and then headed up the hill to touch something built in the early 1600s.
|Inuyama Castle
While photographs were discouraged inside the castle, I do want to tell you that the wooden steps are steep and slick, and there are several flights of them. The castle also tends to shift and shudder in the wind, so if you are afraid of heights, wind or aren't able to climb ladders in bare feet (no shoes in these castles!) then you may just want to admire the architecture from outside.
Castle 1, Inuyama, check!
In my research I had found that there were a total of 12 original castles across Japan. My friend, Shannon, just saw her #8 and will see the other 4 in July. However, I did not make this a quest until I saw Inuyama and then was on my iPhone on the ride home to see where the rest of the castles were. I knew I would not be able to fit all 12 into the timeline I had before my family moved back to the States, but there were only 4 that were national treasures. Maybe I could check all of those off of my list. (Just so you know, you need to keep reading because the internet lied and I found out the hard way.)
But I googled away happily in the back seat, driving away from Inuyama, and realized that Castle #2 was on our way home! Way too excited for my own good, I managed to get my tripmates to agree to a drive-by viewing because the castle would be closed by the time we got there. So, we did the drive-by viewing of Hikone Castle (and there was a Starbucks a mile away, so we caffeinated up and got back on the road. Home was still more than 5 hours away.)
Castle 2, Hikone, check!


text:
Guest accommodation / walking tours
Managed by Mary, a registered bilingual French/English speaking tour guide
Within a 10 minute walk from Killarney bus/train station and town centre, Carranross House bed and breakfast offers spacious rooms with king size orthopaedic beds and great power showers. We are the ideal base for visiting the Killarney National Park which is also within a 10 minute walk and for day tours from Killarney, ie., The Ring of Kerry, The Dingle Peninsula, The Gap of Dunloe etc.
Mary, a native of Killarney & an experienced tour guide, will advise you as to the various day tour options and things to do around Killarney and Kerry. There are several day tours from which to choose & for your convenience we have arranged with the local tour companies for our guests to be collected & dropped off here at no extra cost.
"Best B&B I've stayed in. Friendly, comfortable, clean, good food and Mary couldn't have been more friendly."
Check out more great reviews on Trip Advisor >
Just some reasons to stay with us…
- Walking distance from bus / train station, town centre and shopping outlets
- A 10 minute walk from Killarney National Park entrance
- Quiet location as set back from the main Park road
- Local tour companies pick up & drop off at our b&b
- Free wi-fi
- Free off street car park

subset: commoncrawl


text:
- Accessories
- Art, Photo & Music
- Automotive
- Beauty
- Clothing & Apparel
- Computer & Electronics
- Games & Toys
- Home & Garden
- Office Supplies
- Pets
- Travel
Autodesk coupons + extra cash back
Is designing in your blood? Get the services of Autodesk now and be amazed at how it can transform your ideas into realistic designs that won't cost you a fortune. Make life easier for your architectural and design hobbies. Save time thinking about how you can create your design. Invest in Autodesk and each masterpiece is sure to amaze its target. What are you waiting for? Let out the genius in you. Make the right designer choice with Autodesk now. More information available at autodesk.com.au
Autodesk Cashback
6% Cashback†
Invite friends, earn referral bonus
Invite your friends to enjoy the savings and receive 5% of their earned cash back, up to $50 for each referral.


text:
Changing how you think about
How do you know your patients will be safe - today and tomorrow?
Are you always looking in the rear view mirror at reams of data showing the number of harms suffered last week, last month, last year?
Where do you get your information about safety?
We have something to help you answer those questions………
Introducing the measurement and monitoring of safety e-guide
'Better questions safer care'
Our MMSF learning report utilises the experiences of the 4 RIBs (Regional Improvement Bodies) commissioned by The Health Foundation and covers:
To register for our monthly calls and/or events - please click here


text:
Wish to receive our project updates / connect to our sales team?
Like to check out our SkyWorld Property Gallery?
At SkyWorld, we enhance the living experience of the city communities. We believe our strength lies within our employees and they are our most valuable asset. To harness the best talents to work within a lean and effective team, we ensure you a challenging experience through our Staff Engagement Program, that provides a positive and conducive working environment that fosters good teamwork, builds long-term relationships, encourages creative yet effective ideas, drives empowerment and most importantly, allowing employees to excel in their professional career. With our award winning culture in place, new joiners will feel comfortable and welcomed which inspires them to be productive and excel at their job with the support of our effective New Recruit Buddy Program.
We are proud and honored to be recognized as the Best Employer (2018) & Best Gen Y Employer in Malaysia (2017) by Aon Best Employers Award, this further strengthens our position as one of the Best Employers in Malaysia.
Drop us an email by completing the form below. We would like to hear from you.


text:
Look for a clog if your pool filter pump does not work, pumps water slowly or doesn't effectively clean the pool. Pool filter pump manufacturers recommend looking at a few sources to identify a clog or what seems to affect the pump similar to a clog. What you may think is a clog can be another issue. Once the problem is identified, the manufacturers recommend solutions to resolving it.
Check the Filter
A pool filter may clog when the filter is dirty or damaged. Open the cover on the pump; the filter usually sits in the middle. Remove the filter and check if it appears dirty or damaged. Try spraying the filter with a water hose before replacing it. If the dirt doesn't easily come off the filter or it shows signs of damage, you may need to replace it.
Intake Clog
You may find the source of the clog in the intake hose. Clear the obstruction by discharging the hose into the pool. Reinstall the hose and check if the pump works correctly.
Intake Line Leak
If the intake line is not clogged, you may still find the source of the problem there if the hose has a leak. Looks for any signs of damage on the hose. Replace the hose if you find damage. Replacement hoses are available through your pool or filter dealer or pool supply retailers.
Other Sources of Clogs
Filter pumps and pools come with specific instructions on how to install the nozzle and strainer connections. If reversed, the pool may not operate correctly and can appear or become clogged. Once reversed, the pump may start to work effectively. Inspect the strainer screen for debris and remove. Tighten the hoses and hose clamps on the pool; while you may suspect a clog, the cause may be an improper connection.
More Articles
- Clean a Lawn Mower Engine Fuel Line
- Repair a Garden Sprayer
- Vacuum a Doughboy Pool
- Repair a Hole in a Garden Hose
- Fix a Mantis Tiller That Won't Start
- Troubleshoot the Honda GX 160
- Loss of Pump Pressure in a Pool
- Close an Inground Swimming Pool
- Attach a Garden Hose to Weed Killer
- Use Polaris Pool Cleaner
- Install an Anti-Drain Check Valve for a Leaking Sprinkler
- Troubleshoot an Onga Pool Pump


text:
Miller Park Investigation in Milwaukee, Wis.
Thornton Tomasetti provided emergency response services and conducted a structural failure investigation and collapse analysis of a 500-foot tall mobile crane that collapsed onto the seating bowl of Miller Park (Milwaukee Brewers' baseball stadium) during its construction. Thornton Tomasetti personnel remained on-site for approximately five months performing stabilization and damage assessment services. In addition, an exhaustive structural investigation was performed including three-dimensional finite element analyses (linear, nonlinear, and dynamic).
Follow these topics:
Play Ball!Our Projects - Apr 3, 2016 Milwaukee-Wisconsin Journal Sentinel: The Big Blue crane collapse at Miller ParkMedia Mention - Jul 14, 2014


text:
Tips for Improving Family Stress
The stresses and strain Of everyday life creates a stressed atmosphere for the whole family. Your main objective is to create a joyful home for both kids and your spouse. There are techniques to create a happy family.
Balance Home and Work Life
It helps to find the right balance between work and home because it reduces stress. For those who work unusual shifts, they become fatigued and have no time to rest. Your body needs to relax so that you have the ability to undertake daily activities correctly. You might try asking your employer for a more flexible and reasonable changes or seeking to modify your job profession to a more comfortable one.
Life has lots of challenges. Marriage problems, family issues, self-esteem issues, financial and emotional challenges are just a few of the difficulties that life has to offer. When these problems occur, it is highly advisable to speak to someone or seek guiding and counselling. You could talk about your challenges over dinner or when having family fun times. It is necessary to make time for your kids and partner each day so as to enhance communication.
Talk to somebody
Communication is crucial and When you talk to the ideal individual, a number of your problems could be solved. Nowadays, the internet has come in handy with the availability of online counselors if you do not trust people around you. Discussing lifts, a person's mood and can put stresses or worries off. It's stated that a problem shared is half solved. If your problem is noticed by someone else, they are able to see it in a different way and provide advice wherever necessary.
Make Time For Your Partner
Parents are always active working hard for your family so that the children and other relatives are as comfy as you can. Making time for your partner could bring joy into the family because the partner will feel valued and return the favor. Nobody would want to get ignored or taken for granted. Those simple quiet dinner dates, picnics over the weekend and chats before sleeping could do magic to your relationship.
Show Your Service
Reputation by each other's Time during these tough phases in life bonds the household in ways. Whenever there are activities in your kid's school, show up and give maximum support. Love and support should always be the order of the day in the households.
Appreciate Quality Time Together
Coming up with those family events which create beautiful memories is a smart way to spend time with Your loved ones and revel in the fun moments. Family get together, vacations, weekly dinner arrangements and praying together as a family are few of the ways in Which you can spend time with your loved ones.…


text:
We received over 1,000 entries for our annual members-only edition of Clubhouse. With so many wonderful poems, stories and drawings to choose from, the 2012 Members' Mag is one of our best ever!
Here are some of our favorite pieces we couldn't fit in the print edition.
To That in Which I Poured Myself
Emily, 15, from Virginia
A Tale of Two Blings
Katie and Sidra, both 11, from New Hampshire
Noah, 15, from Utah
God's Promise
Emily, 11, from New Hampshire
Wooton's Backyard
Serena, 10, from Minnesota
Good Morning
Ashlee, 11, from Florida
Kaylyn, 14, from Colorado
Word Search: Finding Fruit
Evalyn, 10, from Pennsylvania
Life as a Seed
Mikaela from California
Adventures of Average Boy: Goody New Shoes
Sammie, 12, from Oregon
Wooton's Random Ramblings: Mystery Box
David, 12, from Alberta
Case of the Frozen Thermometer
Cade, 10, from Illinois
The Supertown Heroes
Trinity, 10, from Virginia
Genevieve, 12, from California
Regan, 12, from Pennsylvania
Maria, 10, from California
Emily, 11, from Ohio
Sabrina, 13, from Florida
Caleb, 9, from Louisiana
Cassandra, 9, from California


text:
Search for help on enabling JavaScript
Jazzmania is the Nashville Jazz Workshop's Annual Jazz Party and Fundraiser. All proceeds benefit the Nashville Jazz Workshop, and go to support the Workshop's mission of enriching people's lives through classes, performances, and special events for the community.
SPECIAL NOTE: The online auction is now open for bidding. If you have a Jazzmania ticket you should have received a text or email with your personal bidding link (if you didn't receive this, email us). If you don't have a ticket you can visit the online auction page, sign up for a free account, and start bidding!
Jazzmania is the Jazz Party of the Year, with great live jazz from some of the city's finest musicians.
This year's artist lineup is sensational!
Headlining the bill is the legendary Jeff Hamilton Trio.
Before forming his own trio, drummer Jeff Hamilton was a member of the Oscar Peterson Trio and the Ray Brown Trio, and is a co-founder of the Clayton-Hamilton Jazz Orchestra. Pianist Tamir Hendelman and bassist Jon Hamar are powerhouse jazz artists in their own right. The trio has visited the Jazz Cave several times over the years, playing to sold-out audiences. It will be a special treat for a larger Nashville audience to hear them at this great event, often dubbed "the Jazz Party of the Year."
Another special treat will be the appearance of vocalist Dara Tucker and her band! Dara has become a favorite of Nashville audiences with her impeccable vocal style and delivery, wide-ranging repertoire, and creative compositions. Joining Dara will be trumpeter Rod McGaha, bassist Greg Bryant, drummer Josh Hunt, and pianist Matt Endahl.
The combination of the Jeff Hamilton Trio and Dara Tucker will make this year's Jazzmania an evening to remember!
Patrons will also be treated to a performance by a group of talented students from the NJW Young Artists Program: Saxophonist Jacob Kitchen, Trumpeter Mayur Gurukkal, Pianist Tyler Bullock, Bassist Baily Johnstone, and drummer Slade Moore.
Jazzmania also includes a live and silent auction, with unique and desirable items such as vacation packages, tickets to top events, special dinner packages, in-home concerts, and fine artwork.
The Silent Auction features the Small Works art collection along with many dining and entertainment packages and unique experiences. The silent auction is now online and open for bidding!
All Jazzmania ticket holders should receive an email and/or text with a personalized link (no login needed) to start bidding. (If you have a ticket but haven't received this link, send us an email and we'll get you set up!)
If you're unable to attend the event but would like to bid on silent auction items, simply go to the mobile bidding site and create a free account with a login and password.
The Live Auction this year will feature some fabulous items and packages, include a unique New York Experience, Four Seasons vacation package, special dinner and concert experiences, and a beautiful original work by noted artist Mildred Jarrett.
Another special element of Jazzmania is the the NJW Heritage Award, presented annually to a person who has made major contributions to the jazz community over an extended time. Congratulations to last year's winner, Kirk Whalum. The audience listened raptly to video tributes from many of Kirk's friends and colleagues from all over the world.
Please join us on October 20 for great music, food, and more!
Phone 615-242-JAZZ (5299) Email firstname.lastname@example.com
Web: www.nashvillejazz.org


text:
Nixu is a growth company operating in the cybersecurity sector. Our long history in the industry and a clearly defined growth strategy make us an interesting investment.
Nixu is a cybersecurity services company on a mission to keep the digital society running. Our passion is to help organizations embrace digitalization securely. Partnering with our clients we provide practical solutions for ensuring business continuity, an easy access to digital services and data protection. With Nordic roots, but based in four continents, we serve enterprise clients worldwide.
Nixu publishes annually a half-year financial report and financial statements and provides revenue information of the first and the third quarter, an annual report and stock exchange and press releases.
In the organization of its administration, Nixu adheres to Finnish legislation such as the Limited Liability Companies Act and the Securities Markets Act, as well as the company's Articles of Association. In the preparation of its annual financial statements and half-yearly reports, Nixu follows the International Financial Reporting Standards (IFRS).
Nixu shares are listed on the Nasdaq Helsinki stock exchange. Nixu has one share series. Each share entitles to equal voting and dividend rights. The shares have no voting restrictions or cutters. Nixu's shares were entered into the Book-Entry Securities System maintained by the Euroclear Finland on January 26, 2000. The shareholder register of Nixu is managed by Euroclear Finland Oy at Urho Kekkosen katu 5 C, 8th floor, Helsinki.
Nixu is committed to active and transparent communications with all of its stakeholders. The company's communications are based on facts and transparency. It is also systematic, honest, equal and timely. The aim is to provide a truthful description of the company's operations, operating environment, strategy, targets and financial performance.
Communications regarding the transfer of Nixu Corporation to the Official List of Nasdaq Helsinki Stock Exchange will be published here.
Notice is hereby given to the shareholders of Nixu Corporation to attend the Annual General Meeting of Shareholders to be held on Thursday, April 25, 2019, beginning at 4:00 PM EET at Dipoli at the following address: Otakaari 24, 02150 Espoo, Finland.
Nixu's Capital Markets Day will be held on September 26, 2019. Read more about the event and register now!
Get cybersecurity news and blogs delivered straight to your inbox


text:
Main Services
EDDM (Direct Mail Services)
EDDM is an inexpensive method to reach potential customers via saturation mail without the need for mailing lists, permits and all the associated fees that come with direct mail advertising. With EDDM, you can market to every household and business in a selected USPS mail route without having to apply different names and addresses to every piece via variable data. Plus the cost of a EDDM mailing is a fraction of the cost of regular direct mail advertising.
Rob Hill
Founder of Printing USA, Inc.
Chip Dutton
Rick Hill
Brad Bitner
Customer Service
MIke Bernard
Senior Press Operator
Lance Bell
Graphic Designer and Digital Pre-Press
About Us
Family owned and operated since 1982, Printing USA has grown from a small print/copy shop to a full service, one stop advertising resource, occupying a 10,000 sq. ft. facility in south Orlando. From 1982 until 1992 we were located in a 1,500 sq. ft. store-front in a shopping center on Oakridge Rd. In 1992 we bought and moved to our current facility, a 10,000 sq. ft. free standing building on South Orange Blossom Trail, where we continue to grow and expand to meet the needs of our customers. We are very proud of our front lobby, which has a small printing museum. Some of the equipment we used as we began our printing journey to present day, is displayed there, as well as antique printing equipment, including an original 1849 Washington press. We invite you to come by to see how printing has evolved throughout history, and our history.
Shop Info
- One Stop Shopping We have learned over the years that our customers love and deserve convenience. Our complete line of related services include printing, signs, promotional products, advertising specialties and mailing services, which provide all the convenience needed for our valuable customers.
- Dependability 2019 marks our 37th year in business. We know that many of our customers depend on us to deliver when promised. We have built our successful business by doing just that.
- Quality Another reason for our success over the last 36 plus years is that we deliver a top quality product. Our motto is "If we would not buy it, don't sell it to our customers".
Printing USA File Upload Instructions
- If your file is less than 20 megabytes, please attach your file to an email, and send to: email@example.com.
- If your file is larger than 20 megabytes, we have an FTP site, ftp://ftp.printingusainc.com, when connected please place your file in the INCOMING folder. For the FTP site, user name is customer and password is upload. (Please send an email to let your Customer Service Representative know of a completed upload).
- You may also use HighTail, please send to email@example.com and let your Customer Service Representative know that you have sent us a file.
- We also use Dropbox, please contact your Customer Service Representative for more details.


text:
CORK flooring is a unique material that has a host of benefits other than being beautiful. The retro appeal of cork floors has seen a recent spike in popularity, and rightly so. It is definitely a material to consider, so read on to look at some of the benefits of choosing a cork floor.
The Great Insulator
Cork flooring is a fantastic insulator of both noise and heat. This attribute makes it a fantastic choice around the home, as it eliminates the dreaded bare feet on cold laminate scenario. Also, if your home is prone to heavy footfall, you will be blessed with a quiet, sound dampening flooring that does not bark at you if you are wearing shoes.
Durable and Spongy
Cork is a very flexible material, and lends itself perfectly to an area where you will be standing around in a lot. Think of a gym room or a kitchen. Cork flooring works as a padding for your feet, its shock absorbing qualities provides gentle comfort. Imagine a common scenario where somebody accidentally drops a glass cup or porcelain plate, the danger of stepping on razor sharp shards is removed with the softer density of Cork. The durability of cork flooring makes it super resistant to wear and tear, and any scuffs or scrapes blend into corks natural pattern.
No Longer A Fortune
Cork is generally cheaper than your typical hardwood timber floor. With advances in technology Cork can now be installed in a variety of flooring types, from floating veneered products, to solid 8mm glue-down Cork. In addition to the cheaper material cost, you can now also save money on the installation, thanks to innovations in manufacturing. With today's floating Cork floors, the simple click-lock system means anybody can install this type of flooring without any floor laying experience.
A Floor For Life
One of the biggest advantages of choosing cork flooring is its longevity. Cork can withstand a lot of punishment over the years and still keep its springy nature and natural aesthetic. If a heavy object dints the Cork, its elasticity means it will retain its original form. Raw Solid Cork is also available, this requires an on-site sand and finish which allows endless options for customised colours and finishes. The biggest advantage of Cork flooring is its impact to the environment, Cork flooring is a completely sustainable, renewable material, as it is harvested from the bark of cork trees which regrows every 9 years.


text: Sponsored Links: The Authority Newspapers Today October 1, 2016 The Authority Mobile app is out! Please visit Google Playstore, search for "Authority News" and click on "install" to download free of charge. Today's top stories: Nigeria's journey @ 56: South East faces worst frustration - https://goo.gl/bLWYWC Nigeria @ 56: South-South demands return to the regions - https://goo.gl/ZzBiKI Ohanaeze 'bombs' Buhari over assets sales - https://goo.gl/uA2VhI Man sues SARS for destroying family house in Anambra - https://goo.gl/4g6gVY Missing IMT car: Corruption is fighting back - Engr Mmamel - https://goo.gl/GU1II3 Why Faleke wasn't declared Kogi Gov - S'Court - https://goo.gl/P2xP4Z Today's AUTHORITY ICON on Page 3 is Michael Taiwo Akinwunmi - https://goo.gl/TYkFpl For the full list & profiles of THE AUTHORITY ICONS from inception till date, click below: http://authorityngr.com/authorityicon?all See extracts of today's AUTHORITY Newspaper: Please ask your vendor for a copy of THE AUTHORITY Newspapers for other stories. You can also: Visit THE AUTHORITY website: www.authorityngr.com Like THE AUTHORITY on Facebook: Authorityngr Follow THE AUTHORITY on Twitter: @Authorityngr Follow THE AUTHORITY on Google Plus: Authority Newspapers Join THE AUTHORITY BBM Channel: C0034EF18 Download THE AUTHORITY App on Google Playstore: Authority News This post first appeared on Linda Ikeji's Blog. Read the original story here.


text:
Autumn is a fantastic time to visit Hendricks County. The brilliant colors and natural beauty of fall lends itself to all sorts of family-friendly activities, and this weekend, there are a ton of events throughout the county to get people outdoors for some fall fun.
Let's take a look.
Fall Colors Run/Festival
Runners and walkers, kick off the weekend at McCloud Nature Park outside of North Salem on Saturday at 9 a.m. with the Fall Colors Run. Enjoy a one-mile, 5K or 10K course along the McCloud trails, surrounded by the colorful fall foliage that makes the park one of the premier spots in Indiana to visit in the fall. All participants will earn themselves a commemorative winter hat and can enjoy refreshments around the campfire afterward.
For more information, including how to register for the Fall Colors Run, click here.
Immediately following the Fall Colors Run (or from 10 a.m. to 4 p.m. on Saturday, if you're not a runner or walker), the Fall Colors Festival takes place at McCloud Nature Park, featuring all sorts of activities for the whole family. Enjoy some archery, learn how to throw an atlatl (an ancient hunting weapon), climb a tree, find some peace through yoga, decorate a pumpkin, make a candle, tackle the heron-themed McCloud Prairie Maze, go on a hayride, play some yard games and much more.
The Fall Colors Festival -- and the vast majority of its activities -- is absolutely free to attend. For everything you need to know about the festival, click here.
Farm Fun at Beasley's Orchard
You haven't done fall in Hendricks County if you haven't been to Beasley's Orchard in Danville. From 10 a.m. to 6 p.m. on Saturday and Sunday, enjoy the Minions-themed Beasley's Corn Maze, go on a hayride out to the pumpkin patch and pick your own pumpkin, pick apples fresh off of the trees, and fire apples through an air compression cannon!
Additionally, Beasley's offers a huge Barnyard Bonanza area where kids and adults can bounce on a giant jumping pillow, navigate the spider web climber, play some tether ball and corn hole and much more.
If you are a police officer, firefighter, paramedic or active member of the U.S. military, this is a great weekend to head out to Beasley's. The weekend theme is Hometown Hereos, and they are offering discounts to first responders and their families.
For more information about Beasley's Orchard, click here.
Trick-or-Treat at the DAC/Haunted Hargrave Hall
On Saturday from 5 to 6:45 p.m., bring your kids out to the Danville Athletic Club (or "the DAC," as it's commonly referred to around here), for some trick-or-treating, games, and prizes. Additionally, there is a costume contest in three age categories -- 0 to 3, 4 to 7, and 8 to 12 -- so get those Halloween costumes together! This event is free to attend.
Also during those hours, Danville Parks & Recreation will have the lights on inside Haunted Hargrave Hall for the little ones who don't wish to see their young lives flash before their eyes. If the kiddos want to explore the haunted house with the lights on, it's a mere $3 per person. Candy and prizes will be given away throughout the non-scary adventure.
From 7 to 11 p.m. on Saturday, however, it's lights off and game on inside Haunted Hargrave Hall! Prepare for your heart to jump into your throat and the adrenaline to course through your veins for just $7 per person at the door (or purchase a fast pass for $12 at the door and skip the lines!). Save some money by purchasing your tickets online at least a day ahead of time for $5 per person or $10 for a fast pass.
To learn more, including how to order tickets online, click here.
Nehemiah Ranch Fall Harvest Days
The final night of the Nehemiah Ranch Fall Harvest Days in Avon is Saturday from 6 to 10 p.m., and this is an event that my family has really enjoyed in the past. Nehemiah Ranch is tucked away in the woods and instantly transports its visitors from the hustle and bustle of daily life to the serenity of nature.
Fall Harvest Days allow families to roast hot dogs and make s'mores around a campfire, take a free hayride, pick your own pumpkin (kids ages 12 and under), listen to some live music and sip hot cocoa. All of it is free to enjoy. Freewill donations are accepted.
To read about my family's experience there a couple years ago, click here.
Even More Activities
I merely touched on the tip of the leaf when it comes to all the great fall activities taking place in Hendricks County, where Fall Break lasts all season long. For our guide to even more fall fun, click here or check out the Visit Hendricks County Events Calendar.
What are you doing this weekend?


text:
Our Work in Child Welfare
Partners for Our Children works to improve the lives of vulnerable children and families in Washington state, especially those touched by the child welfare system. But we can't do that alone. That's why we work closely with partners to get the right information into the right hands – those making important decisions about child welfare practice and policy.
Child Welfare Fast Facts
- In 2017, approximately 8,800 children lived in out-of- home care.
- Nearly 51% of children who entered out-of- home care were under five years of age.
- 57% of children were reunified with parent(s) within three years of entering out-of- home care.
- Half of all children who entered out-of- home care in 2014, exited within 15 to 18 months.
Current Projects
Latest Briefs and Reports
Federal policy
RECENT RULING ENABLES HHS TO DISCRIMINATE ON THE...Brief Dec 04, 2019
Child welfare
The Indian Child Welfare Act: The Gold Standard...Brief Jul 16, 2019
State policy
Partners for Our Children 2019 state policy...Brief Jan 09, 2019
Budget & revenue


text:
Susan Lovenburg is a 19-year resident of Davis. She has been a trustee of the Davis Joint Unified School District since 2007.
Susan with daughters - Sarah, Meg and EmmaSusan has three daughters, ages 18, 20 and 26. The youngest daughter graduated from Davis High this June and will attend the University of San Francisco in the fall. Susan's middle daughter is a junior at Willamette University and her oldest lives and works in Los Angeles, having graduated from UC San Diego in 2012.
A longtime parent volunteer prior to her election to the School Board, Susan has continued her community service as a member of Soroptimist International of Davis and a board member for Yolo CASA (Court Appointed Special Advocates).
Together with city, county and school elected representatives and community members, Susan co-founded Saving California Communities to advocate for state governance reforms, as well as the Yolo County Chapter of the Brady Campaign to Prevent Gun Violence.
Susan is past president of the Yolo County School Boards Association and a member of the delegate assembly of the California School Boards Association. She recently served on CSBA's Legal Alliance Adequacy Committee and currently serves on the 2016 Annual Education Conference Planning Committee.
In 2011, Susan joined the staff of California Forward, a non-profit, nonpartisan organization working to identify common sense steps Californians can take to make government work. CA Fwd accomplishes this through broad consultation and analysis of critical problems facing the state, and then identifies nonpartisan reforms that can make a difference. Susan serves as director of the Partnership for Economic Prosperity, and manages the California Economic Summit project. In partnership with CSBA, she also staffs a Local Control Funding Formula Collaborative of fifteen school districts and two county offices of education. The Collaborative meets four times a year working through challenges and barriers to successful implementation of the LCFF.
Susan holds a bachelor of science degree from Cornell University and a master of library science from Syracuse University. She completed the California School Boards Association Masters in Governance program in 2011, and continues to participate in experienced board member training.


text:
Hotel and tourism at Bond
With more than 12 million visitors annually, there is no better place to study Hotel and Tourism Management than the Gold Coast- the tourism capital of Australia.
The Bachelor of International Hotel and Tourism Management at Bond will prepare you for professional and managerial roles in the tourism industry, including hotels, resorts, airlines, restaurants, theme parks, cruise lines, destination marketing and management organisations and events. Bond has strong relationships with Marriott, Accor, Hilton, InterContinental, Sheraton, Wyndham and many other international tourism organisations to ensure that you have the opportunity to see real industry projects and undertake internships throughout your studies.
Bond Business School Experience Day | Tuesday, 22 October, 2019 | 9am - 2.30pm Register now Year 10, 11 and 12 high school students are encouraged to attend.
See how we rateDownload brochure
Unparalleled access to the industry
What our students say
Key features
- Opportunity to enhance your learning experience with international study tours, work experience and study abroad programs
- Meet all the right people at Bond Business School's networking events, speaker series, pitch nights, demo days and more
- Work in small supportive groups with dedicated teachers who bring a wealth of international academic and industry experience to the classroom
About the program
The Bachelor of International Hotel and Tourism Management is designed to prepare you for professional and managerial roles in the global tourism and hotel industries. Emphasis is placed on asset, revenue and human resource management within tourism and hotel businesses. Students enrolled in the program will complete 400 hours of work experience in a hotel, tourism or event environment to complement the theoretical knowledge gained through the program.
|On Campus
|6 semesters (2 years)
|Starting semesters
|Program type
|Bachelors Degree
|Study area
|Hotel and Tourism
|Program code
|CRICOS code
|Credit for prior study
|For more details on applying for credit, please contact the Student Business Centre: [email protected]
The Bachelor of International Hotel and Tourism Management is a qualification accepted nationally and internationally by leading hotel and tourism industry employers. Graduates often attain roles in:
- Airlines
- Cruises
- Hotels
- Government
- Research
- Resorts
- Restaurants
- Theme Parks
The Bachelor of International Hotel and Tourism Management program comprises 24 subjects, as follows:
View the Program structure and sequence plans
University core subjects (3)
- CORE11-001: Critical Thinking and Communication
- CORE11-002: Leadership and Team Dynamics
- CORE11-003: Ethical Thought and Action
Plus: CORE11-004: Beyond Bond: Professional Development and Community Engagement
Faculty core subjects (12)
- ACCT11-100: Accounting for Decision Making
- HRTM12-203: Rooms Division Management
- HRTM12-204: Contemporary Issues in Hospitality and Tourism Management
- HRTM12-205: Strategic Pricing and Revenue Management
- HRTM12-206: Tourism Analysis and Evaluation
- HRTM12-212: WHS Risk Management
- HRTM12-220: Sustainable Tourism and Indigenous Culture
- IBUS12-251: Concepts of Strategy
- INFT11-120: Digital Transformation of Business
- LAWS10-100: Business Law
- MGMT13-305: Human Resource Management
- MKTG11-100: Marketing
Alternate Choice (3)
Choose three (3) subjects from the following options.
- HRTM11-100: Wine Studies
- HRTM12-201: International Food and Beverage Management
- HRTM12-209: Event Operations
- HRTM12-214: Adventure Tourism
Students must complete 400 hours work experience as a graduation requirement, in approved industry/positions.
Electives (6)
Students can choose 6 General Elective Subject (60CP).
General elective – A subject that may be chosen from any subject on offer across the University provided requisites, enrolment restrictions and any other faculty requirements have been met.
Major (6)
Students may choose to complete an optional Major by selecting all six (6) of their general electives within one of the following majors.
Students may choose to complete an optional Minor by selecting four (4) of their general electives within one of the following minors plus two (2) additional subjects from the undergraduate General Electives.
Students may choose one of the following opportunities to use one of your elective subjects to have an international experience or an Internship opportunity provided you meet the requirements. Please discuss with the Enrolment Advisor in the Student Business Centre.
- Study Tour
The Study Tour requires approval from the Faculty. (Please note that Additional Costs are associated with this subject).
- Business Internship
The Internship requires a GPA credit average and requires approval from the Faculty. An application can be sought from the Career Development Centre.
- Individual Study Topic
The Individual Study Topic is only available in exceptional circumstances and requires Program Director's approval.
The following opportunities are also available to Bond Business School students.
- BUSN13-730: Business School International Semester
Students will be undertaking study at approved overseas institutes offering an international internship experience program, facilitating both academic study courses and practical industry internship placement
- Bond International Exchange Program
Current Bond students have the opportunity to study at one of 80+ leading universities in our network of exclusive international partnerships, for one or two semesters.
Important note for first semester students
You will be registered in the Bond Business Mentoring Program designed for all new undergraduate students; please be advised the first scheduled gathering is in the Bond Business School orientation, If you require further information please email [email protected]
The below fees are based on an average per semester cost, fees may vary based on which subjects are selected and the number of subjects enrolled in for each semester:
- 2019 fees: $17,160 per semester (based on 4 subjects per semester)
- 2020 fees: $17,360 per semester (based on 4 subjects per semester)
When considering the fees associated with your studies, keep in mind that Bond's accelerated schedule means you can finish your degree sooner and be out in the workforce up to a year earlier than if you went to another university.
This time saving also represents a substantial reduction in accommodation and living costs, plus a full year of extra earnings.
Find out your financing options and other costs to consider.
Academic requirements
Applicants with recent secondary education (within the past two years)
Applicants must meet the University's general minimum admission criteria.
In the January semester 2019 there were fewer than five OP/ATAR offers made in this program hence no comparative information on previous OP/ATAR intake scores is available.
Visit our student profile if you are interested in the profile of all students who commenced undergraduate study at the Faculty in the January semester 2019.
International Secondary School students
For more information for International Students, including the International Baccalaureate, please go to the International Secondary School equivalency page.
Alternative entry pathways
For those applicants who do not currently have the required academic qualifications, there are a number of alternative entry pathways:
- Bond University Tertiary Preparation programs
- Bond University Diploma programs
- Other institutional Tertiary Preparation Programs
- Vocational education and training qualifications (Certificate IV and above)
- Prior higher education experience (at this university or another)
- Special Tertiary Admissions Test (STAT)
- Professional or para-professional qualifications/experience
- Employment experience verified by a statement of service from the employer stating the position title and length of service and a very brief statement on the tasks undertaken.
For more information on what is required please visit our how to apply page.
English language proficiency requirements
As tuition is delivered in English, all students will be required to provide documented evidence of the required level of proficiency in the English language. Read more detailed information on English language proficiency requirements for university study.
Credit for prior study
Subject credits may be awarded for previous studies. To apply for credits, you will need to submit academic transcripts including detailed subject outlines/course descriptions for each relevant subject and/or certified copies of testamurs to the Office of Future Students. Please refer to how to apply for credit for more information
Key dates
How to apply
In order to apply for study at Bond University, you will need to complete the online application form. To find out more about the application process please refer to how to apply.
Business majors
Complete six subjects from one of the following majors:
This major will appeal to those enterprising individuals with an eye towards their own start-up business, or who are keen to work as an "intrepreneur" inside an organisation. Students will gain skills in ideation, creativity and innovation in planning a business and developing a viable business model. Through experiential learning and support from industry experts, students will graduate with contemporary and best practice knowledge, insights, and strategies with which to implement effective management practices and bring a business idea into fruition.
You must complete the following four subjects:
- ENFB13-300 Entrepreneurial Growth
- ENFB13-302 Managing Innovation
- ENFB13-304 New Venture Planning
- ENFB13-305 Venture Capital and Private Equity
Plus two subjects from the following options:
The International Business major prepares students for a variety of roles in today's fast-changing and ever more competitive international business environment. The subjects offered in this major develop theoretical and practical knowledge, and analytical and entrepreneurial skills, that underpin success in international trade.
You must complete the following four subjects:
- ECON13-303 International Trade
- FINC13-307 International Finance
- IBUS11-310 Doing Business Globally
- IBUS12-250 International Business
Plus two subjects from the following options:
This major provides students with a holistic body of knowledge, skills and strategies that are critical to long-term organisational success. The scope of subjects covered in the Management Major provides students with the ability to identify strategic problems and develop viable, value-add solutions for a range of organisational issues that present at different organisational levels.
You must complete the following four subjects:
- IBUS12-251 Concepts of Strategy
- MGMT13-305 Human Resource Management
- MGMT13-311 Change Management
- MGMT13-321 Negotiation
Plus two subjects from the following options:
This major develops students' knowledge, skills, and practical experience in designing and implementing market-driven, value-creation marketing strategies underpinned by a sound understanding of consumer and buyer behaviour. The focus is placed on how to conduct market research, analyse findings, and communicate these to management.
You must complete the following four subjects:
- MKTG12-301 Consumer and Buyer Behaviour
- MKTG13-302 Promotional Design and Planning
- MKTG13-303 Market Research and Analysis
- MKTG13-320 Strategic Marketing
Plus two subjects from the following options:
You must complete the following two subjects:
Alternate Choice: You must also complete one of the following two subjects:
- ADVT11-100 Advertising Principles and Practice or
- PUBR11-100 Public Relations Principles and Practice
Plus three subjects from the following options:
- ADVT12-200 Direct Response and Writing for Advertising
- ADVT12-241 Advertising Communications and Channel Planning
- ADVT13-101 Advertising, Brand Image and Cultural Space
- MKTG13-312 Internet and Social Media Marketing
- PUBR12-235 Media Relations
- PUBR12-250 Public Relations Writing
- PUBR13-312 Public Relations Campaigns and Strategies
Students taking the Marketing Communication major MUST Choose Advertising Principles and Practice (ADVT11-100) before taking either Advertising, Brand Image and Cultural Space (ADVT13-101) or Media Relations (PUBR12-235). Students wanting to take either Media Relations (PUBR12-235), Public Relations Writing (PUB12-250) or Public Relations Campaigns and Strategies (PUBR13-312) MUST first complete Public Relations Principles and Practice (PUBR11-100).


text:
Burglar Alarm Systems & House Alarms
Alert Electrical are delighted to offer a great range of burglar alarms systems from highly established brands incuding Texecom, Honeywell ADE, Pyronix, Scantronic and Visonic.
If you have any queries regarding our intruder alarms then please contact our team today and we will be happy to help.
Aico Ei Professional 12v Optical Smoke DetectorExcl. Tax: From £23.97 Incl. Tax: £28.76


text:
Example #1: Aggregate multiple SharePoint lists
Example #3: Dynamic color-coding by field values
Example #4: Using resources
This calendar is configured to group events by rooms. It is also configured to prevent users from double-booking a room.
Example #2: Mini-Calendar
This Calendar is configured to display the list calendar1 as a mini-calendar. Click on a day marked bold to see details of all the events scheduled on this day.


text:
Typically, I'm not a huge fan of 'best things to do in..." and "top places to visit in..." posts, because they tend to be redundant and rarely offer up new, insightful information. However, I've noticed a sharp lack of helpful posts on the best things to do in Egypt. Sure, everyone knows about the Pyramids and the Library of Alexandria, but there are so many hidden treasures in Egypt that few people ever encounter. And while I'm no authority on the subject, living in Egypt for a year exposed me to most of what the country has to offer. I visited most of these sites multiple times, and can provide detailed information that other reviews tend to leave out. My goal is to provide a fresh list of 10 amazing locations you should visit in Egypt, that you likely don't know about.
Egypt is a perfect location to visit right now, too. While most people tend to be under the misconception that the country is very dangerous currently, the opposite is true (though keep in mind, opinions of 'safety' and 'comfort' vary). Egypt is between revolutions, they have had no recent riots or upheaval, and tourism is at an all-time low. That means that prices are dirt-cheap, there are no tourists to block your view, and you can get to nearly any site with ease. Still not convinced? Take a look at my top 10 sites, and see if your mind has been changed.
1. The White Desert
The number one, without-a-doubt, most-amazing place I visited in Egypt was the White Desert. Never heard of it? You're not alone. Part of the intrigue of the White Desert is that it's relatively unheard of; in the hundreds of miles of sand and rock structures, you'll encounter perhaps 5 other people, at most. The White Desert is a vast expanse of desert with dozens of unique locations. I refer to it as 'the yellowstone of the desert', for while it doesn't host geothermal features, it has several incredibly unique locations in a relatively small geographical area. The desert boasts strange rock formations, fossils, oases, and most famously, a desert covered in smooth white sandstone and giant white mushroom rocks. To visit the White Desert, you must hire a tour from a local Bedouin group (there are several companies available), and they take you out camping for anywhere from 1-14 days. On our trip, we hiked up giant black rocks, collected fossils and strange stones, swam in a real oasis, watched the sunset on massive white rocks, ate traditional Egyptian food, and had an overwhelmingly unforgettable trip. The White Desert, is by far, my favorite place I've been in this whole world. Convinced yet?
You can see more photos from my trip to the White Desert, here. (scroll to center)
2. Karnak, Luxor
While Karnak is not unheard of to most people, it's so incredible to visit that I couldn't help putting it on this list. Karnak is about 11-hours by train or a 1-hour flight south of Cairo, in the river-city of Luxor. Luxor is home to many other temples, ruins, and tombs (Valley of the Kings, Temple of Luxor, and the Temple of Hatshepsut, to name a few), but Karnak tops them all. Karnak is a large temple complex, absolutely brimming with hieroglyphs, original paint (!), statues, obelisks, and any other traditional Egyptian carving and structure you can think of. The place is completely overwhelming; never, in any other location on earth, have I felt so surrounded in history as I did at Karnak. Plus, it's right inside the city - just a few minutes walk or a taxi ride away from your hotel! Luxor in general is the best tourist city in Egypt, but if you visit, put Karnak at the top of your list.
You can see more photos from my trip to Karnak, here.
3. Marsah Matruah
These sparkling turquoise waters may look like something fresh off a Caribbean island, but they are actually at home off the coast of the Mediterranean in Egypt! Marsah Matruah (See also: Mersa Matrouh, Marsa Matrouh, Marsa Matruha - all thanks to the Arabic letter 'heh' which isn't translatable) is a small city about 3-hours west of Alexandria, near the border of Libya. Because of it's relative isolation and general lack of public knowledge, it is a [stunning] diamond in the rough. The waters are absolutely pristine, the beaches are empty, and the air is the perfect temperature. The above photo features Agiba beach, which is my personal favorite, but Cleopatra beach is another local favorite. In addition to simply lounging around in the sun, there is snorkeling, boating, and cliff-jumping available all within 30-minutes of the main city. The one caveat? To be respectful of the local culture, it is necessary that women are fully covered while swimming (as seen above). I wore leggings and a t-shirt, which was appropriate for the setting.
You can see more photos from my trip to Marsah Matruah, here. (scroll to bottom)
4. Daashur and Saqqara
While everyone knows about the pyramids in Egypt, they typically only think of those at Giza. The pyramids at Giza and the Sphinx are, in my opinion, the most over-rated attractions in Egypt (not as a result of their history, or magnificence, to be sure). They are right in the city-center, surrounded by buildings, and crowded with vendors who pester you to buy things. I ate pizza at a Pizza Hut less than 100-feet from the Sphinx; there is no vast sandy expanse behind it to take ride your camel off into the sunset on. What most people typically see images of, without realizing, is the Red Pyramid at Saqqara. The Red Pyramid is the second-largest pyramid (only 10-feet shorter than the Great Pyramid of Giza), but located about 1-hour outside of the main city of Cairo. Less than 5-minutes from Saqqara is the pyramid complex of Daashur, home to the Bent Pyramid (pictured above). There are several other smaller pyramids at both these sites, all of which are nearly devoid of tourists and vendors. The Bent Pyramid has a really awesome temple at the entrance as well, and the Red Pyramid has a newly-opened museum called the Serapeum which shows where sacrificial bulls were killed and buried to honor the gods. Pretty cool stuff. I can also pretty much guarantee that these sites will be empty as well. The people in the background of the photo above were the rest of the staff from my school, and in the three times I visited each site, I never saw more than 5 other people wandering around. And though I wasn't a big fan, you can go inside the Red Pyramid to the chamber in the center (read, climb down a 500-ft, 4-ft tall crawl space with no light in 110-degree heat) for free.
You can see more photos from my trip(s) to Daashur and Saqqara, here.
5. Abu Simbel
Abu Simbel is an imposing temple complex in the southernmost part of Egypt, just a few kilometers from the border with Sudan. While I never personally made it to Abu Simbel (though I desperately wanted to), my roommate went to the complex for a few days, and it is highly regarded by my other Egyptian staff/friends as an incredible site to visit. If you can't tell from the image above, the statues and temple itself are absolutely massive, and their are plenty of original hieroglyphs to view. Due to it's relative isolation south of Lake Nasser (just 50-km north of the border of Sudan), most tourists don't make it down for a visit; the closest city is Aswan, so if you're staying there, you should definitely make the trip a few hours south to this site. Abu Simbel is actually two separate sites - the temple for Ramses II (above) and a second smaller temple dedicated to the goddess Hathor and Queen Nefertari. Both host original carvings and artwork, and are considered some of the most beautiful remaining temples in Egypt.
6. Philae
Yet another amazing site I missed out on, Philae is a temple complex on an island in Lake Nasser. Due to it's closeness to Abu Simbel in southern Egypt, they are a good pair to plan on seeing together. Philae can only be visited by boat, meaning that you achieve a lovely view on your way to the temple, as well as when you land. The temple showcases some incredible bas-relief carvings and hieroglyphs, alongside massive colonnades and huge halls. This is definitely a place you want to visit if you're a history buff or art fan, as there is no shortage of either in the complex. My roommate claims it as her favorite temple she visited in Egypt - which is saying a lot, because she saw them all!
7. The Khan Il-Khalili, Cairo
|Img source
|Img Source
|Img source


text:
Journal of Obesity
Volume 2013, Article ID 874981, 11 pages
Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry
1Department of Food Metabolism and Nutrition, Korea Food Research Institute, 516 Paekhyon-dong, Pundang-ku, Songnam, Kyongki-do 463-747, Republic of Korea
2Research and Development Center, Nong Shim Co., Ltd., Seoul 156-709, Republic of Korea
3Department of Food & Nutrition, Yonsei University, Seoul 120-749, Republic of Korea
4Department of Food Science & Nutrition, Yongin University, Kyongki-do, 134 Samka-dong, Chuin-ku, Yongin, Kyongki-do 449-714, Republic of Korea
Received 18 December 2012; Revised 7 March 2013; Accepted 11 March 2013
Academic Editor: Xu Feng Huang
Copyright © 2013 Min Jung Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The present study aimed to identify key metabolites related to weight reduction in humans by studying the metabolic profiles of sera obtained from 34 participants who underwent dietary intervention with black soybean peptides (BSP) for 12 weeks. This research is a sequel to our previous work in which the effects of BSP on BMI and blood composition of lipid were investigated. Sera of the study were subjected to ultra performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and the data were analyzed using partial least-squares discriminate analysis (PLS-DA) score plots. Body mass index and percent body fat of the test group were reduced. Levels of betaine, benzoic acid, pyroglutamic acid, pipecolic acid, N-phenylacetamide, uric acid, L-aspartyl-L-phenylalanine, and lysophosphatidyl cholines (lysoPCs) (C18:1, C18:2, C20:1, and C20:4) showed significant increases. Levels of L-proline, valine, L-leucine/isoleucine, hypoxanthine, glutamine, L-methionine, phenylpyruvic acid, several carnitine derivatives, and lysoPCs (C14:0, PC16:0, C15:0, C16:0, C17:1, C18:0, and C22:0) were significantly decreased. In particular, lysoPC 16:0 with a VIP value of 12.02 is esteemed to be the most important metabolite for evaluating the differences between the 2 serum samples. Our result confirmed weight-lowering effects of BSP, accompanied by favorable changes in metabolites in the subjects' blood. Therefore, this research enables us to better understand obesity and increases the predictability of the obesity-related risk by studying metabolites present in the blood.
1. Introduction
Obesity is considered a perilous disease because, besides being widespread, it is the primary risk factor for the development of various physical, mental, and social disorders such as cardiovascular diseases, type 2 diabetes, obstructive sleep apnea, certain types of cancers, mental depression, and social stigmatization [1, 2]. Causes of obesity are numerous, including imbalance between calorie intake and expenditure, genetic susceptibility, emotional reasons, and environmental factors such as life style [3].
Thus appropriate diet and good eating habits are the foundation for physical fitness. Certain Korean traditional fermented soybean foods; fermented soybean and fermented soybean pastes, notably Chungkukjang and Doenjang, may help to alleviate or fight obesity [4–6]. Research results by Yang et al. indicated that feeding chungkukjang decreased blood pressure and serum levels of lipids in rats [7]. Soh et al. tried to identify the weight-reducing effects of fermented soybean products, chungkukjang and doenjang, by analyzing the hepatic mRNA expressions of enzymes related to fatty oxidation [4, 5]. However, most of these research results are unable to correlate substances responsible for antiobesity as soybean and soybean products contain numerous compounds with functional properties, for example, saponins, isoflavones, and proteins [7–10].
In addition, to take full advantage of the health-promoting effects of fermented soybean products, physiological effects of purified soybean proteins and soybean peptides are extensively studied by numerous researchers. Nagasawa et al. reported that soybean protein could lower the triglyceride content and fatty acid synthase mRNA level in adipose tissue [11, 12]. In human studies with black soybean peptides (BSPs), Kwak et al. asserted that the peptides were effective in reducing body weight, body fat mass, and plasma leptin levels of obese subjects [13]. Kim et al. were successful in purifying and identifying adipogenesis inhibitory peptides from black soybean protein hydrolysates [14].
In our previous paper, we reported that obese research subjects who had taken BSP supplementations experienced significant reduction in body weight, body mass index (BMI), and body fat mass. Serum leptin levels were found to be significantly reduced in these research participants [15]. BSPs used for the experiment are analogues of doenjang (fermented soybean paste) peptides. They were produced by hydrolyzing whole soybean protein with proteases extracted from Aspergillus oryzae, the main fungi responsible for doenjang fermentation [16]. The peptide sequences were analyzed to be identical to those of the glycinin fraction of black soybean or to those (data not shown) found in doenjang. Our research group was able to discover that the commonly required structural features for the biological activity of some antiobesity peptides were the hydrophobic moiety and proline [17]. Moreover, identifying specific peptides associated with antiobesity is often difficult because of contaminating peptides with similar physicochemical properties. In another study with humans, we were able to identify 3 lysophosphatidylcholines (lysoPCs), lysoPC C14:0, lysoPC C16:0, and lysoPC C18:0, as the potential markers for overweight/obesity through metabolomic profiling of plasmas of overweight/obese men [18]. Liver and serum metabolites of obese and lean mice fed on high-fat or normal diets revealed that, in addition to lysoPCs, levels of lipid metabolism intermediates such as betaine, carnitine, and acylcarnitines, as well as some branched-chain amino acids (BCAAs), contributed to the discrimination between the 2 groups [19].
The aim of the present study is to elucidate the anti-obesity effects of BSPs by studying the metabolic profiles of serum obtained from subjects who have undergone dietary intervention with these peptides. To our knowledge, this is almost the first metabolomic study that attempts to elucidate the weight-reducing effects of soybean peptides in humans. Since metabolites present in the blood directly reflect the body's physiological changes, we expect to obtain further insight into the health-promoting effects of soybean peptides, as well as that of fermented soybean products that have long been known as healthy food in Korea.
2. Materials and Methods
2.1. Subjects Selection and Composition of BSPs
Details on the general characteristics of all volunteers subjects, which were recruited by public advertisement and the diet intervention protocol for obesity by BSP, are provided in our previous paper [15]. The study subjects were 34 overweight/obese subjects aged 19–65, who were interested in losing weights. Both the BSP supplement and placebo were obtained from Nongshim Co., Ltd. (Seoul, Korea). Amino acid analysis showed that BSP, manufactured by hydrolyzing whole soybean proteins with proteases from Aspergillus oryzae was composed of low-molecular weight amino acids of less than 10,000 kDa. Some of the peptide sequences analyzed by LC-MS were as follows: NLQGENEEEDSGAIVTVK, VSIIDTNSLENQLDQMPR, KEQQQEQQQEEQPLEVR, EQQQEQQQEEQPLEVR, GNPDIEHPETM, LDTSNFNNQLDQTPRVF, NQEQEFLKYQ, RLLLLLGWLLIIVGVILLVGSTK, KEQQQEEQQEEQPLEVR, IIDTNSLENQLDQMPR, LDTSNFNNQLDQNPRVF, EQQQRQQQEEQPLE, and PMDYYSDYDDNADDYFDDADDSDR (see supplement data are available online at http://dx.doi.org/10.1155/2013/874981). These amino acid sequences coincided with those of glycinin subunits present in black soybean (http://www.ncbi.nlm.nih.gov/).
2.2. Sample Preparation for Analysis
We collected 150 μL of serum from the 34 study participants to which 300 μL of cold acetonitrile was added to extract soluble metabolites. After shaking for 30 min at 4°C, the samples were centrifuged at 10,000 rpm for 10 min at 4°C. The supernatants, which were freeze-dried and stored at −70°C, were dissolved in 20% methanol just before ultra performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) analysis [17, 20].
2.3. Characteristics of Participants
The characteristics of the participants observed in previous papers [15] are described in short as follows: all subjects who participated in the present research project were healthy individuals without any signs of apparent ailments such as heart diseases or diabetes (Table 1). Moreover, according to the criteria of the Korean Society of the Study for Obesity (KSSO), most of the participants were classified as overweight and obese (BMI value of 23–25 and ≤25, resp.). Mean age, BMI (), and percent body fat () of the test group consisting of 34 subjects (15 men and 19 women) were , , and , respectively. Total energy intake () and expenditure () of the test group were kcal/day and kcal/day during the 12 weeks of testing, respectively. Carbohydrates comprised the largest portion of energy intake (61.5–62.0%), followed by fat and protein. No noticeable differences in eating behavior were apparent between before and during the research period, except for the individuals in the test group, who were taking one packet of dietary supplements containing 4.5 g of BSP after each meal.
2.4. Serum Analysis by UPLC-Q-TOF-MS
Serum extracts were analyzed on a UPLC-Q-TOF-MS instrument (Waters, Milford, MA, USA), as described by Kim et al. [17]. The Q-TOF-MS was operated in positive electrospray ionization (ESI) mode, with a scan range of m/z 50–1,000. Cone voltage was 30 V, capillary voltage was 3 kV, and scan time was 0.2 s, with an interscan delay of 0.02 s. The source temperature was set at 110°C, while the desolvation flow was set at 700 L/h; the desolvation gas temperature was set at 300°C. The MS was calibrated using sodium formate, and leucine enkephalin was used as lock mass. The concentration of leucine enkephalin was 200 ρM and the flow rate was set at 5 μL/min. As quality control, a mixture of 5 standard compounds (4-acetoaminophenol, caffeine, sulfadimethoxine, terfenadine, and reserpine) was injected after running every 8 samples.
In the MS-MS experiments, argon was used as collision gas, with the collision energy alternating between 10 and 30 eV. The MassLynx software version 4.1 (Waters Inc.) was used to control the instrument and calculate accurate masses. Peaks were collected using a peak width at 5% height, 1 s, a noise elimination of 6, and an intensity threshold of 70. Data were aligned with a mass tolerance of 0.04 Da and a retention time window of 0.2 min. All spectra were aligned and normalized to an external standard. Assignment of metabolites contributing to the observed variance was performed using the ChemSpider (http://www.chemspider.com/) and Human Metabolome Database (http://www.hmdb.ca/).
2.5. Data Processing for Statistical Analyses
Statistical analyses were performed on the data using SIMCA-P+ software (version 12.0.1, Umetrics Inc., Umeå, Sweden). Partial least-squares discriminant analysis (PLS-DA) was used to visualize discrimination among samples and an internal 7-fold cross-validation was carried out to estimate the performance of the PLS-DA models. Goodness of the fit was quantified by and and the predictive ability was indicated by . In addition to cross-validation, model validation was also performed by a 200 times permutation test. Analysis of variance was performed to determine statistical significance by using SPSS 11.5 (SPSS Inc., Chicago, IL, USA) at a significance level of [17, 19].
3. Results and Discussion
3.1. Characteristics of Participants after Dietary Intervention
After completion of the test period, BMI and percent body fat of the test group were found to be and %, respectively. Both indices were consistently lower compared to the start of the intervention, suggesting effectiveness of soybean peptides on weight reduction. Triglyceride levels of the test group were lower after 12 weeks. Initial and final serum triglyceride concentrations of the test group were mg/dL and mg/dL, respectively, (). Total cholesterol levels () were almost unchanged at start and the end of the trial. In contrast to the total cholesterol concentration, HDL-cholesterol levels () exhibited a marked increase at the end of the dietary intervention. The concentration of the macromolecular complex increased from mg/dL to mg/dL.
Our result in good agreement with other similar nutritional intervention studies in which the effect of soybean protein on mice or humans was investigated [21–23]. Rho et al. revealed that mice with diet-induced obesity-induced mice showed reduced body weight as well as improved lipid profiles when fed with BSP for 28 days, as compared to casein-fed mice [21]. Jang et al. also proved that BSP has anti-obesity and triglyceride-reducing effects on mice fed with a high-fat diet for 13 weeks [22]. In human studies with soybean-based meals, Allison et al. showed that 100 obese people exhibited significantly reduced body weight, body fat mass, total cholesterol, and LDL cholesterol after participation in a randomized controlled trial for 12 weeks [23]. The results obtained here indicate that our intervention study could be a good example for further human metabolomics studies.
3.2. Serum Metabolic Profile
Sera of the participants in the test group were collected at the initial and final stages of the diet intervention and analyzed by UPLC-MS. The resulting base peak intensity chromatograms obtained in positive ion (ESI+) mode are shown in Figure 1. Next the obtained data were applied to a PLS-DA score plot to elucidate the existence of class distinction between the stages. The PLS-DA score plot showed a separation between 0 week and 12 week samples along the axes corresponding to the first 2 PLS-DA components (Figure 2(a)). The variation in () was 51.0%, while the variation in () was 80.6% which predicts 47.2% of the variation in response to (%) for 2-component model. The permutation test with a permutation number of 200 was performed and indicated a intercept value of 0.701 and a intercept value of −0.116. Thus, the result shows that 0-week samples and 12-week samples could be clearly differentiated from each other by the primary or secondary component with the goodness of fit of the data. This result indicated that the study subjects who took BSP for the period of 12 weeks experienced changes in the profiles of the serum metabolites.
3.3. Identification of Possible Biomarkers
The S plot of metabolites along the axes corresponding to the combined weight and reliability correlation indicates the contribution of individual metabolites to the separation of the 2 samples. The higher or lower the value of of the metabolites, the greater the degree of contribution made by these metabolites to the discrimination of the test samples. Metabolites with positive values correspond to those with decreased serum levels caused by diet intervention, whereas metabolites with negative value correspond to those whose level increased during the study period of 12 weeks. All 758 metabolites detected with our UPLC-MS system are shown in the S plot (Figure 2(b)), and the metabolites with marked numbers are the ones that were identified using MS-MS. Variable importance in the projection scores (VIP scores) as well as the normalized fold changes of the metabolites due to dietary intervention is also presented in Table 2. The normalized levels of the metabolites in the sera collected at weeks 0 and 12 of the intervention were statistically analyzed by using the -test.
Eleven serum metabolites, that is, betaine (), benzoic acid (), pyroglutamic acid (), pipecolic acid (), N-phenylacetamide (), uric acid (), L-aspartyl-L-phenylalanine (), and lysoPCs containing C18:1 (), C18:2 (), C20:1 (), and C20:4 (), showed a significant increase during 12 weeks of peptide intervention, whereas 20 metabolites, that is, L-proline (), valine (), L-leucine/isoleucine (), hypoxanthine (), glutamine (), L-methionine (), phenylpyruvic acid (), propionylcarnitine (), butyrylcarnitine (), L-hexanoylcarnitine (), L-octanoylcarnitine (), palmiotylcarnitine (), linoleylcarnitine (), and PCs containing C14:0 (), PC16:0 (), C15:0 (), C16:0 (), C17:1 (), C18:0 (), and C22:0 () showed decreased levels. Aminobutyric acid (), carnitine (), N-phenylacetamide (), 2-phenylglycine (), phenylalanine (), arginine (), tyrosine (), and tryptophan () showed little changes in the concentration with fold changes being in the range of 0.8–1.2. Among 38 metabolites identified, L-proline, betaine, and lysoPCs containing C16:0, C18:0, C18:1, and C20:1 exhibited VIP values greater than 3.0, indicating a high correlation with discrimination between the samples collected at weeks 0 and 12 of diet intervention. In particular, lysoPC 16:0 with a VIP value of 12.02 is esteemed to be the most important metabolite for evaluating the differences between the 2 serum samples (Figure 3). A similar result was revealed by Kim et al. who showed that, in mice with diet-induced obesity, lysoPC 16:0 was the single most important metabolite related to obesity [17]. Using Pearson's correlation analysis, relationships between the changes in the levels of major metabolites were elucidated and are shown in Figure 4. The changes in lysoPC 16:0 levels were positively related to lysoPC 18:0 (, ), while that of lysoPC 18:1 was found to be related to lysoPC 18:2 (, ). The change in the lysoPC 22:0 level was closely related to lysoPC 18:0 (, ). The change in the hypoxanthine level was related to glutamine (, ). The change in levels of linoleylcarnitine and pipecolic acid was related to palmityl carnitine (, ) and betaine (, ), respectively. Obesity is known to be accompanied by distinct changes in the metabolite composition of serum, including lipids, amino acids and other small compounds. Of particular interest is lysoPC, as numerous studies have suggested that lysoPC levels are closely associated with ailments such as endothelial dysfunction, inflammation, and atherogenesis, as well as obesity [24–26]. Since lysoPC has a relatively short half-life, it is thought to be metabolic intermediates that are produced during the formation or breakdown of other lipids. These compounds are formed in the liver and carried by albumin and lipoproteins to various parts of the body. According to Croset et al., unsaturated lysoPCs are associated with albumin rather than lipoproteins [27]. LysoPCs are known to account for 5–20% of all phospholipids in the serum [28]. Our studies showed that sera of subjects after 12 weeks of diet intervention exhibited slight reductions in the levels of both total triglycerides and percent body fat though those of lysoPCs did not show a definite trend and were shown to be dependent on the types of fatty acid chains esterified to the glycerol moiety. A positive relationship existed between the changes in the levels of lysoPCs with saturated fatty acids at sn-2 position of phosphatidylcholine, while the levels of lysoPCs with unsaturated fatty acid esterified at sn-2 position increased during the diet intervention. Our result is in accordance with those of other studies with of obese men, pigs, and monozygotic twins [19, 24, 29]. The serum levels of lysoPCs 14:0 and 18:0 in obese men and lysoPC18:0 in pigs with diet-induced obesity were higher than those of controls, whereas that of lysoPC 18:1 was decreased in obese men, while no change was observed in the lysoPC 16:0 level in pigs [24]. The studies with monozygotic twins showed a close correlation between increased lysoPC levels and obesity [29]. However, other studies showed that the relationship between lysoPC levels and obesity is unclear and that further studies are needed to elucidate the role of lysoPCs in weight reduction [24].
Betaine is another compound that was found to be increased in the serum of participants during the diet intervention program. In addition to its function as an organic osmolyte and methyl donor in the remethylation step of the methionine-homocysteine cycle, betaine is known to prevent or reduce the accumulation of fat, especially in the liver, and, thus, is called a lipotrope [30]. Researchers have found that betaine helps to reduce hepatic cholesterol and phospholipids in rats fed a high-fat diet [31], relieve hyperlipidemia [32], and increase the production of carnitine [33]. These findings might explain why betaine levels were increased in subjects who had undergone dietary intervention with BSP. Nevertheless, mechanisms behind elevation of betaine level following BSP consumption are uncertain at this moment and thus need further researches.
The levels of carnitine were found to be increased by dietary intervention, too. However those of acylcarnitines, that is, propionylcarnitine, butyrylcarnitine, L-hexanoylcarnitine, L-octanoylcarnitine, palmitylcarnitine, and linoleylcarnitine, were decreased. Carnitine is a quaternary ammonium compound that is naturally produced in the human body from L-lysine and L-methionine or supplied through dietary sources [34]. Carnitine synthesis is known to occur both in the kidney and liver and carnitine is transported in the blood for use by muscles. Acylcarnitines are synthesized in the cytosol, which then enter the mitochondria where the acyl group is removed from carnitine to form acyl-CoA. The acyl group is subsequently catabolized in 2-carbon units by β-oxidation inside the mitochondrial matrix. Thus, carnitine and acylcarnitines are considered important biomarkers for obesity ([35], see http://en.wikipedia.org/wiki/Carnitine). Reports indicate that carnitine can diminish the risk of obesity caused by a high-fat diet in mice [36] or in obese humans [37]. Some studies show that carnitine might indirectly work to reduce the weight in obese subjects by increases in muscle mass or reduction in fatigue [38].
One of the possible reasons for the increased levels of carnitine in our study might be due to the relatively high amounts of precursor amino acids present in the dietary supplements taken by the participants for 12 weeks. Moreover, the decreased levels of acylcarnitines were quite unexpected and, thus, further studies are needed to better understand the study outcome. One of the possible reasons for the observed effect might be the reduction in percent body fat and serum triglyceride levels in our test subjects, which could have resulted in a decrease in the levels of acylcarnitines, as these compounds are substrates for the biosynthesis of acylcarnitines. Rho et al. assert that the depletion of fatty acids due to energy production via β-oxidation and increased citric acid cycle activity might lead to decreased transportation of fatty acids in adipose tissue in participants who had taken BSP along with their normal diet [21].
The levels of BCAAs notably valine and leucine/isoleucine were found to be lower after diet intervention, along with those of aromatic amino acids such as tyrosine, phenylalanine, tryptophan, and other amino acids, for example, arginine, L-methionine, proline, and L-glutamine. The decrease in the levels of BCCAs and aromatic amino acids with weight loss is consistent with recent findings where close relationships were established between changes in blood BCAAs levels and obesity (http://www.umm.edu/altmed/articles/carnitine-l-000291.htm). However, the underlying mechanisms of the relationship between obesity and increased BCAA levels is not yet fully understood. BCAA levels might increase simply because obese individuals eat more or because of increased protein catabolism as the result of insulin resistance. Jensen and Haymond [39] reported that proteolysis is increased in obese individuals, whom the antiproteolytic action of insulin is impaired. Two studies, one with obese and lean humans, and the other with animals on high-fat diet with and without BCAA supplementation or on normal diet revealed that blood BCAA levels were consistently higher in obese subjects. The level of valine is especially associated with a decreased BCAA catabolism rate which is attributed to the development of obesity-associated insulin resistance. According to a study by Kim et al., higher levels of tyrosine and tryptophan (or, with regard to our study, the decreased levels of aromatic amino acids in subjects who had undergone diet intervention) in obese individuals might be due to their competition for transport into cells with large neutral amino acids [19].
Uric acid is synthesized by the enzyme xanthine oxidase; xanthine and hypoxanthine serve as substrates. Among several metabolites, uric acid levels were increased in the blood of study participants, while those of hypoxanthine were significantly decreased. Since all of our study participants experienced a decrease in body weight, percent body fat, and blood triglyceride levels, this result is unexpected as uric acid is widely known to be an indicator for obesity. Several reports asserted that elevated uric acid levels (as well as those of hypoxanthine) are closely related to an increase in body weight and body fat as well as obesity-related diseases such as diabetes, abdominal obesity, endothelial dysfunction, inflammation, and subclinical atherosclerosis [40]. Thus, further studies are needed to clarify the differences between our results and those of others. In addition, the roles of other metabolites identified in this research, for example, benzoic acid, L-aspartyl-L-phenylalanine, pipecolic acid, pyroglutamic acid, N-phenylacetamide, phenylpyruvate, and aminobutyric acids, which were all increased as the result of BSP consumption are not understood at this time and, thus, requires further studies to elucidate the underlying mechanism. We already suggested some mechanisms of ageing and obesity by proposing regulating pathways in mice with metabolomics approaches [17, 41]. In this study, however, the data are not sufficient to discuss the mechanisms of the BSP-induced antiobesity effect in humans. Further studies are needed to accumulate data for studying the antiobesity mechanisms for some nutraceuticals.
Numerous beneficial functions of soybean peptides have been identified, including hypolipidemic effects, improvement in endothelial function, insulin resistance, and weight loss. Since obesity is associated with the development of some major human diseases, it is considered a serious problem worldwide. The main cause of obesity is the imbalance between energy intake and energy expenditure, which leads to an increase in body weight and percent body fat. Nagasawa et al. showed that soy protein isolates control the gene expression in adipose tissue and effectively regulate adipogenesis, which results in a lower triglyceride content in adipose tissue [11, 12]. Our result confirmed weight-lowering effects of BSP, accompanied by favorable changes in metabolite levels in the blood of subjects who have participated in this research. Therefore, the results of this study allow us to better understand obesity and its related diseases, increase the predictability of obesity-related risks by studying metabolites present in the blood, and assess the therapeutic effects of antiobesity agents.
Conflict of Interests
The authors including Nongshim declare that they have no conflict of interests.
Authors' Contribution
M. J. Kim and H. J. Yang contributed equally to this work.
This work was supported by "Food Functionality Evaluation program" under the Ministry of Agriculture, Forestry, Fisheries and Foods, and partly by "Personalized Programmed Foods" project of Korea Food Research Institute. The authors sincerely thank Hookeun Lee at Lee Gil Ya Cancer and Diabetes Institute, College of Pharmacy, Gachon University, who helped them to analyze amino acid sequences of black soybean peptide.
- D. W. Haslam and W. P. James, "Obesity," The Lancet, vol. 366, no. 9492, pp. 1197–1209, 2005. View at Google Scholar
- E. E. Calle, C. Rodriguez, K. Walker-Thurmond, and M. J. Thun, "Overweight, obesity, and mortality from cancer in a prospectively studied cohort of U.S. Adults," The New England Journal of Medicine, vol. 348, no. 17, pp. 1625–1638, 2003. View at Publisher · View at Google Scholar · View at Scopus
- J. P. Adams and P. G. Murphy, "Obesity in anaesthesia and intensive care," British Journal of Anaesthesia, vol. 85, no. 1, pp. 91–108, 2000. View at Google Scholar · View at Scopus
- J. R. Soh, D. H. Shin, D. Y. Kwon, and Y. S. Cha, "Effect of Cheonggukjang supplementation upon hepatic acyl-CoA synthase, carnitine palmitoyltransferase I, acyl-CoA oxidase and uncoupling protein 2 mRNA levels in C57BL/6J mice fed with high fat diet," Genes and Nutrition, vol. 2, no. 4, pp. 365–369, 2008. View at Publisher · View at Google Scholar · View at Scopus
- J. R. Soh, D. Y. Kwon, and Y. S. Cha, "Hepatic gene expression profiles are altered by dietary unsalted Korean fermented soybean (Chongkukjang) consumption in mice with diet induced obesity," Journal of Nutrition and Metabolism, vol. 2011, Article ID 260214, 10 pages, 2011. View at Publisher · View at Google Scholar
- D. Y. Kwon, S. M. Hong, I. S. Ahn, M. J. Kim, H. J. Yang, and S. Park, "Isoflavonoids and peptides from meju, long-term fermented soybeans, increase insulin sensitivity and exert insulinotropic effects in vitro," Nutrition, vol. 27, no. 2, pp. 244–252, 2011. View at Publisher · View at Google Scholar · View at Scopus
- J. L. Yang, S. H. Lee, and Y. S. Song, "Improved effect of powders of cooked soybean and chungkukjang on blood pressure and lipid metabolism in spontaneously hypertensive rats," Journal of the Korean Society of Food Science and Nutrition, vol. 32, pp. 899–905, 2003. View at Google Scholar
- M. Messina, "Modern applications for an ancient bean: soybeans and the prevention and treatment of chronic disease," Journal of Nutrition, vol. 125, no. 3, pp. 567–569, 1995. View at Google Scholar · View at Scopus
- V. R. Young, "Soy protein in relation to human protein and amino acid nutrition," Journal of the American Dietetic Association, vol. 91, no. 7, pp. 828–835, 1991. View at Google Scholar · View at Scopus
- H. J. Yang, S. Park, V. Pak, K. R. Chung, and D. Y. Kwon, "Fermented soybean products and their bioactive compounds," in Soybean and Health, H. A. El-Shemy, Ed., p. 59, Intech, Rijeka, Croatia, 2011. View at Google Scholar
- A. Nagasawa, K. Fukui, T. Funahashi et al., "Effects of soy protein diet on the expression of adipose genes and plasma adiponectin," Hormone and Metabolic Research, vol. 34, no. 11-12, pp. 635–639, 2002. View at Publisher · View at Google Scholar · View at Scopus
- A. Nagasawa, K. Fukui, M. Kojuma et al., "Divergent effects of soy protein diet on the expression of adipocytokines," Biochemical and Biophysical Research Communications, vol. 311, no. 4, pp. 909–914, 2003. View at Google Scholar
- J. H. Kwak, J. H. Lee, C. W. Ahn et al., "Black soy peptide supplementation improves glucose control in subjects with prediabetes and newly diagnosed type 2 diabetes mellitus," Journal of Medicinal Food, vol. 13, no. 6, pp. 1307–1312, 2010. View at Google Scholar · View at Scopus
- H. J. Kim, I. Y. Bae, C. W. Ahn, S. Lee, and H. G. Lee, "Purification and identification of adipogenesis inhibitory peptide from black soybean protein hydrolysate," Peptides, vol. 28, no. 11, pp. 2098–2103, 2007. View at Publisher · View at Google Scholar · View at Scopus
- J. H. Kwak, C. W. Ahn, S. H. Park et al., "Weight reduction effects of a black soy peptide supplement in overweight and obese subjects: double blind, randomized, controlled study," Food and Nutrition, vol. 3, no. 10, pp. 1019–1024, 2012. View at Google Scholar
- G. Puski, "Modification of functional properties of soy proteins by proteolytic enzyme treatment," Cereal Chemistry, vol. 52, pp. 655–664, 1975. View at Google Scholar
- H. J. Kim, J. H. Kim, S. Noh et al., "Metabolomic analysis of livers and serum from high-fat diet induced obese mice," Journal of Proteome Research, vol. 10, no. 2, pp. 722–731, 2011. View at Publisher · View at Google Scholar · View at Scopus
- D. Y. Kwon, S. W. Oh, J. S. Lee, H. J. Yang, S. H. Lee, and J. H. Lee, "Amino acid substitution of hypocholesterolemic peptide originated from glycinin hydrolyzate," Food Science and Biotechnology, vol. 11, pp. 55–61, 2002. View at Google Scholar
- J. Y. Kim, J. Y. Park, O. Y. Kim et al., "Metabolic profiling of plasma in overweight/obese and lean men using ultra performance liquid chromatography and Q-TOF Mass spectrometry (UPLC-Q-TOF MS)," Journal of Proteome Research, vol. 9, no. 9, pp. 4368–4375, 2010. View at Publisher · View at Google Scholar · View at Scopus
- W. B. Dunn, D. Broadhurst, P. Begley et al., "Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry," Nature Protocols, vol. 6, no. 7, pp. 1060–1083, 2011. View at Publisher · View at Google Scholar · View at Scopus
- S. J. Rho, S. Park, C.-W. Ahn, J.-K. Shin, and H. G. Lee, "Dietetic and hypocholesterolaemic action of black soy peptide in dietary obese rats," Journal of the Science of Food and Agriculture, vol. 87, no. 5, pp. 908–913, 2007. View at Publisher · View at Google Scholar · View at Scopus
- E. H. Jang, J. S. Moon, J. H. Ko et al., "Novel black soy peptides with antiobesity effects: activation of leptin-like signaling and AMP-activated protein kinase," International Journal of Obesity, vol. 32, no. 7, pp. 1161–1170, 2008. View at Publisher · View at Google Scholar · View at Scopus
- D. B. Allison, G. Gadbury, L. G. Schwartz et al., "A novel soy-based meal replacement formula for weight loss among obese individuals: a randomized controlled clinical trial," European Journal of Clinical Nutrition, vol. 57, no. 4, pp. 514–522, 2003. View at Publisher · View at Google Scholar · View at Scopus
- O. Galili, D. Versari, K. J. Sattler et al., "Early experimental obesity is associated with coronary endothelial dysfunction and oxidative stress," American Journal of Physiology—Heart and Circulatory Physiology, vol. 292, no. 2, pp. H904–H911, 2007. View at Google Scholar
- N. Aiyar, J. Disa, Z. Ao et al., "Lysophosphatidylcholine induces inflammatory activation of human coronary artery smooth muscle cells," Molecular and Cellular Biochemistry, vol. 295, no. 1-2, pp. 113–120, 2007. View at Google Scholar
- G. Schmitz and K. Ruebsaamen, "Metabolism and atherogenic disease association of lysophosphatidylcholine," Atherosclerosis, vol. 208, no. 1, pp. 10–18, 2010. View at Publisher · View at Google Scholar · View at Scopus
- M. Croset, N. Brossard, A. Polette, and M. Lagarde, "Characterization of plasma unsaturated lysophosphatidylcholines in human and rat," Biochemical Journal, vol. 345, no. 1, pp. 61–67, 2000. View at Publisher · View at Google Scholar · View at Scopus
- A. M. Nelson, "Phospholipids and coronary mortality. Use of ratio between phospholipid and cholesterol levels to determine successful treatment," Northwest Medical, vol. 61, pp. 47–52, 1962. View at Google Scholar
- K. H. Pietiläinen, M. Sysi-Aho, A. Rissanen et al., "Acquired obesity is associated with changes in the serum lipidomic profile independent of genetic effects-a monozygotic twin study," PLos One, vol. 2, no. 2, article e218, 2007. View at Google Scholar
- S. A. Craig, "Betaine in human nutrition American," Journal of Clinical Nutrition, vol. 80, no. 3, pp. 539–549, 2004. View at Google Scholar
- K. Sugiyama, H. Akai, and K. Muramatsu, "Effects of methionine and related compounds on plasma cholesterol level in rats fed a high cholesterol diet," Journal of Nutritional Science and Vitaminology, vol. 32, no. 5, pp. 537–549, 1986. View at Google Scholar · View at Scopus
- P. Turpin, "A double blind study of the effectiveness of Beaufor betaine citrate ampules in the treatment of type IV hyperlipidemias," Sem Hop, vol. 61, pp. 2420–2434, 1985. View at Google Scholar
- J. Odle, "Betaine and carnitine," Feed Manage, vol. 47, pp. 25–27, 1996. View at Google Scholar
- A. Steiber, J. Kerner, and C. L. Hoppel, "Carnitine: a nutritional, biosynthetic, and functional perspective," Molecular Aspects of Medicine, vol. 25, no. 5-6, pp. 455–473, 2004. View at Publisher · View at Google Scholar · View at Scopus
- P. Rinaldo, T. M. Cowan, and D. Matern, "Acylcarnitine profile analysis," Genetics in Medicine, vol. 10, no. 2, pp. 151–156, 2008. View at Google Scholar
- E. G. Mun, J. R. Soh, and Y. S. Cha, "L-carnitine reduces obesity caused by high-fat diet in C57BL/6J mice," Food Science and Biotechnology, vol. 16, no. 2, pp. 228–233, 2007. View at Google Scholar · View at Scopus
- Y. S. Cha, J. R. Soh, C. K. Kim, and H. Park, "Effect of carnitine and antioxidant supplementation on carnitine and lipid profiles in trained and non-trained humans," The FASEB Journal, vol. 17, supplement 1, pp. A733–A744, 2003. View at Google Scholar
- J. Y. Yang, S. J. Lee, H. W. Park, and Y. S. Cha, "Effect of genistein with carnitine administration on lipid parameters and obesity in C57B1/6J mice fed a high-fat diet," Journal of Medicinal Food, vol. 9, no. 4, pp. 459–467, 2006. View at Google Scholar · View at Scopus
- M. D. Jensen and M. W. Haymond, "Protein metabolism in obesity: effects of body fat distribution and hyperinsulinemia on leucine turnover," American Journal of Clinical Nutrition, vol. 53, no. 1, pp. 172–176, 1991. View at Google Scholar · View at Scopus
- S. D. Lin, D. H. Tsai, and S. R. Hsu, "Association between serum uric acid level and components of the metabolic syndrome," Journal of the Chinese Medical Association, vol. 69, no. 11, pp. 512–516, 2006. View at Google Scholar · View at Scopus
- N. Son, H. J. Hur, M. J. Sung et al., "Liquid chromatography-mass spectrometry-based metabolomic analysis of livers from aged rats," Journal of Proteome Research, vol. 11, no. 4, pp. 2551–2558, 2012. View at Google Scholar


text:
Images left to Right: super-sized necklace by Strzelec, Wes Johansen on Flycicle and the set of the Waffle Shop.
Saturday, March 6, 2010
GA/GI Where Bold Fashion, Flycicles and Waffles Rule
Rebecca Strzelec, an associate Professor of Penn Sate, Altoona who formerly lived in St. Louis, grew up thinking that the Cardinals were the only team in baseball because of her father's love for the organization. Years later, she has retained her fascination for all things baseball, much of it manifested in bold, colorful, and extraordinary wearable art. You'll see some of her most stunning work built layer by layer in various plastics and photosensitive resins at the GA/GI Fashion show "Pedal to the Metal." Other fashion headliners include our eco-tech founders: Jonano, Amisha Gadani, Neighborhood Academy, Rebellious Nature and Heather Joy Puskarich and Webelowear along with our latest fashion friends Hey Betty! vintage clothing and collectibles/ Kelly Lane Designs "Clothing with color", Little Earth accessories from salvage, LaVerne Kemp hat couture and Jorge Myers hand-painted style.
The Flycicle generated from the mind of Wes Johansen who states: "The future is upon us, but it's not quite how we imagined it. Where are the flying cars and robot wives? This is not the Utopia that was once promised by the slick suited corporations of the fifties. The social and economic problems of society still persist. Technology has proven to be a frustrating savior." You'll enter his visionary installation: Yesterday's Tomorrow on April 2.
The Waffle Shop is a neighborhood restaurant that produces and broadcasts a live-streaming talk show with its customers, operates a changeable storytelling billboard on its roof, and runs a take-out window that sells food from countries engaged in conflict with the U.S. The shop is a public experiment that brings together people from all walks of life to engage in dialogue and the co-production of culture. Starting at 11 pm, after the Geek Art/Green Innovators Festival on April 2, bring in your program or enter wearing your GA/GI gear, blab about your GA/GI "experience" and get $1 off your plate of waffles! Waffle Shop is at 124 S. Highland Avenue 15206.
Images left to Right: super-sized necklace by Strzelec, Wes Johansen on Flycicle and the set of the Waffle Shop.
Images left to Right: super-sized necklace by Strzelec, Wes Johansen on Flycicle and the set of the Waffle Shop.
Posted by greenartists at 10:51 AM
2 comments:
Subscribe to: Post Comments (Atom)


text:
You're probably ahead of the game and have all your holiday shopping done, right? Well, did you remember to get something for the special, furry, four-legged friend in your life? If not, be sure to stop by Bone Appetit in Brownsburg, a shop that focuses on handmade treats, quality food and special gifts for you or your favorite dog-lover. Owner Suzie Lee first opened the shop in Danville, then moved it to Brownsburg two years ago. This owner of a rescued Doberman loves her job — while she's not nearly old enough to retire, she said the shop is like her retirement business because it's so enjoyable. When you stop by, feel free to bring your dog, because they're welcome in the store. You'll find more than two dozen varieties of treats in bulk that you can mix yourself to create a combination specifically for your dog. You'll see ingredients like sweet potato, pumpkin, peanut butter, blueberry and peppermint — the original recipes use all-natural ingredients and are corn, wheat and soy-free. In addition to delectable treats, Bone Appetit carries several brands of dog food that are also corn, wheat, grain, and soy-free. This shop is also the only place in Hendricks County you'll find the Horizon brand of food. Want a gift of more than food and treats? You'll also find treat jars, clothing, leashes, grooming supplies and toys. I was particularly impressed to see locally sourced and smoked bones — the BFE Trading Post of North Salem gets bones from Moody's Butcher Shop and smokes them. Cat owners, don't fret: You can find a few things for your feline friend, as well. Insider tip: Like Bone Appetit on Facebook to learn about fan-only deals each Saturday. Be sure to add Bone Appetit to your holiday shopping list — and stop by after the holidays, too!
Bone Appetit: A Treat for Pets, Owners
Wednesday, December 18, 2013 10:28 PM by Amanda Miller