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A well-equipped library is essential for the proper functioning of every educational institution. The Thoompunkal Ramakrishna Pillai Memorial Library in our college has a collection of around 11,000 books and 32 periodicals and journals. The library has a computerized system with regard to availability, issue, and return of books which facilitates utilization. Library hours are from 9:00 AM to 4:30 PM.
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5 Answers
- David ELv 75 months ago
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Monthly Archives: March 2015
God's Love is Infinite by Fr. Joel
Abraham Lincoln was asked during the civil war, ''if the war is over, and when the south has been subdued and conquered, and has come back into the union, how are you going to treat these rebellious southerners, what are you going to do to them?'' And he answered: '' I am going to treat them as if they had never been away.'' That is exactly the attitude of our God when we repent of our sins; he treats us as if we have never committed any sins.
Today is the fourth Sunday of lent and it is called ''Laetare Sunday'' a Latin word which means ''Rejoice''. Hence the antiphon of today says, ''Rejoice, Jerusalem; be glad for her all you who love her….'' On Laetare Sunday the Lenten discipline is relaxed. The organ and other instruments may be used, and there may be flowers on the altar; the priest may wear rose colored vestments. On a day like this, the Pope will bless a golden rose. He will explain the meaning: The rose is beautiful, but it has thorns. The beauty of redemption will not come without the thorns and pain of the cross.
So, we are rejoicing because of God's infinite love and mercy to us. In spite of our sins, he loves us and forgives us. ''For God so loved the world that he gave his only begotten son, that whoever believes in him may not die but may have eternal life.'' (Jn 3:16). Today we rejoice because our catechumens are getting closer to their baptism and full communion in the church. We rejoice because God never seems to learn his lesson, despite our stubbornness and sins, he keeps loving us, forgiving our sins, and will never give up on us. He never learned his lesson in creation: He created everything good and gave to our first parents, and they paid him with disobedience. He did not learn his lesson when he delivered the people of Israel from slavery in Egypt and did many great things for them, and they pay him back with rebellion. Even today, we who are the new Israel, we are rebellious and sinful, yet he loves us and grants us forgiveness. We have abandon God for the sake of secularism and civilization and we are turning things upside down. As a result, we are committing all kinds of atrocities such as abortion, same sex marriage, divorce, euthanasia, violence, suicide, murder, and all kinds of injustices and hatred to one another. Yet God's love is boundless and he is ready to forgive us. Indeed, his mercy and love is infinite. So we have every reason to rejoice today. Do not just be happy but repent and receive his love and mercy.
The first reading is woeful and the gospel is up lifting. The first reading shows the anger and punishment of God while the gospel shows his love and mercy. We are people of the gospel, people of the New Testament and our God is merciful. Therefore, we must be happy, grateful to him, and take advantage of this opportunity and repent of our sins and receive his forgiveness. His forgiveness is freely given-you do not have to earn it. God loves and forgives us- Do you forgive others? God's love is infinite- do you have limit to your love for others? Love begets love-do you receive love and not give others love?
Jesus Cleansing the Temple by Fr. Joel
Today we read about Jesus chasing people away from the temple for doing business there. The question is if Jesus did that, why do we have festivals or funds raisings in the church? Yes we do have fund raising in the church, but the difference is that those people at the time of Jesus were doing it for personal gains and not for the temple, while ours is done with the intention of raising funds for the church and not for any personal interest. And no one is cheating or stealing from anyone as it was during the time of Jesus.
The first commandment says we should not have any other god except the one and true God. And the second says we should not call his name in vain. The third one is on keeping the Sabbath holy. Then from the fourth to the tenth is about the respect and love of others, beginning with our parents. These commandments instruct us to respect the person of God, his name, his temple, and the people he created. In Jewish tradition, if you respect a person, you will also respect his/her name and everything that is related to that person, for example, spouse, parents, children, house, etc.
That is what these commandments are asking us to do to our God-to love and respect his person and everything that is related to him. The temple is a place where we worship God. It is often called his house or his dwelling place. And all those who come in there to worship him are his children, so, they should be loved and respected because they are related to him. No one would be happy if anything related to him/her is abused by someone else. We like to be loved and respected and this love to be extended to every one of our relations and property.
Now we can see why Jesus was angry and drove people out of the temple. The people turn the temple into a business place where they were cheating and stealing from people who came to worship God for their personal interest. They disrespected God, his temple, and the people of God.
Jesus is still angry with us because we too are doing the same things those people did during his time on earth. Many of us do not love and respect God, his temple, and his people. When we refuse to obey his commandments, we are disrespecting him. If you refuse to come to church, or come late to church for no just reason, or come to church and you are rushing to get out for no good reason, or if you come and you do not pay attention, or you make noise to distract people praying in his temple, you are disrespecting God. When you do not dress decently, or eat and drink too much, or you do not take care of yourself or abuse your body, you are disrespecting the temple of God. If you do not recognize the presence of Jesus in the Eucharist/church, you are disrespecting God. If you help someone and you expect something in return, you are doing business not kindness. When you do contract or job for the church and you charge for excessive pay, you are cheating God. If you refuse to contribute for development and maintenance of church facilities and ministries, you are stealing from God and his temple. When you do not have time to pray or volunteer to participate in the work and ministries of the church, you mean to God. When you do not respect your neighbor, you are disrespecting God.
We need to clean all of these bad attitudes in our live so that our bodies/ temples of God will be purified and be dwelling places for him. The season of lent is good time to clean up this mess in our lives. Pray for forgiveness and ask for the grace to stay clean and holy for God to remain in your life; fast from sin and learn to love other people.


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ARUSHA (Xinhua) – Fourteen migrants, identified Ethiopians, have been found dead in a vehicle Mindu area, near Morogoro municipality, along the Tanzania-Zambia highway.
Morogoro Regional Police Commander Wilbroad Mutafungwa said there were 26 in the vehicle but 14 of them had already died, while five were being treated at the Morogoro Regional Hospital. Seven others under observation.
He said that the aliens were on their way to South Africa, through Malawi or Zambia. The cause of death is not known.
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Indian markets are likely to be marginally higher on Monday tracking moderate gains in global peers. Trends in SGX Nifty suggest positive opening of Indian indices.
Indian stock markets will remain shut on Tuesday on account of Muharram.
Asian stocks eked out modest gains on Monday, amid a cautious market mood as investors pinned expectations on likely stimulus to support growth in the world's major economies, which showed further signs of struggle.
MSCI's broadest index of Asia-Pacific shares outside Japan added 0.1%. Australian stocks edged up 0.1%, South Korea's KOSPI rose 0.8% and Japan's Nikkei was up 0.5%. The Dow rose 0.25% and the S&P 500 edged up 0.1% on Friday.
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Brent crude oil futures gained 0.5% to $61.85 per barrel. The contract had risen 1% on Friday after the Fed said it would act to sustain US economic growth and was on track to gain for the fourth day.


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Espionage, kidnapping, and the dark art of spycraft is as American as George Washington. One of Washington's first acts upon taking command of the Continental Army in 1775 was to hire a spy to go behind the enemy lines and report on British activities in Boston. He devoted a considerable amount of energy to his role as intelligence chief,
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George Washington, Foreign Policy quotes: Against the insidious wiles of foreign influence (I conjure you to believe me, fellow-citizens,) the jealousy of a free people ought to be constantly awake; since history and experience prove, that foreign influence is one of the most baneful foes of Republican Government.
AN UNDERSTANDING OF THE CONSTITUTION'S FOREIGN AFFAIRS POWER. Get exclusive insider information from Heritage experts delivered straight to.
Foreign policy themes were expressed considerably in George Washington's farewell address; these included among other things, observing good faith and justice towards all nations and cultivating peace and harmony with all, excluding both "inveterate antipathies against particular nations, and passionate attachments for others", "steer[ing] clear of permanent alliances with any portion of the foreign world",
"It's a big deal," said David Kris, a senior Justice Department official during the administrations of presidents George.
George Washington and John Adams were America's first two presidents and two of the most vital men that created the American republic. It's not overstating it to say that if both of these men had not been at the forefront of the political affairs of their day, our republic would be very different or not exist at all.
Benjamin Franklin Rodriguez Now Almendarez is now broadcast in 15 markets. We've all heard the quotes from Benjamin Franklin about his concerns that German-Americans would never assimilate. These concerns are as old as the. Benjamin Franklin (1706–1790) was an American statesman, writer, scientist, Benjamin Cromwell Franklin (1805–1873), Texas state senator; Benjamin Joseph Franklin. Benjamin Franklin Rodriguez, the main character
Briefly summarize George Washington's beliefs about political parties. 2. What warning about foreign nations does Washington give in his farewell address? 3. Why do you think Washington was so concerned about these two issues? 4. Evaluate the role of political parties in today's national scene. Were Washington's concerns valid for the future of.
It may also advance plans to reduce the number of diplomats posted to the U.S. Embassy in Iraq as Washington winds down. working on economic or other issues 4 or 5 to 1," said a senior.
. of foreign policy, the new nation faced a combination of unresolved issues and new. When Washington took office, the ongoing conflict between the tribes in the. He appointed Revolutionary War hero George Rogers Clark a general, and.
Briefly summarize George Washington's beliefs about political parties. 2. What warning about foreign nations does Washington give in his farewell address? 3. Why do you think Washington was so concerned about these two issues? 4. Evaluate the role of political parties in today's national scene. Were Washington's concerns valid for the future of.
HalBrands1) Reuben Brigety is dean of the Elliott School of International Affairs at the George Washington University. worked on post-conflict issues at the Center for Strategic and International.
Supporting the participation of three Indian foreign service officers in a two-week seminar on U.S. Foreign Policy at George Washington University. understanding of U.S. foreign policy and domestic.
Mar 2, 2018. President exercises far greater power over foreign affairs than. George Washington, Address to the United States Senate and House of.
"It creates the possibility that someone who is up to speed with the issues, and has a comfortable working relationship. In a presentation last fall at George Washington University, one of the.
Xtc Statue Of Liberty Live NEW YORK — After more than two hours, police apprehended a woman who climbed the Statue of Liberty. U.S. Parks Police evacuated Liberty Island after the woman, identified as Therese Patricia Okoumou, Question Answer; Name something you would wan't to run over with your lawnmower. Rocks, Animals, Poop, Hose, Toys, Sprinkler: Name a movie that
To announce his decision not to seek a third term as President, George Washington presented his Farewell Address in a newspaper article September 17, 1796. George Washington Frustrated by French meddling in U.S. politics, Washington warned the nation to avoid permanent alliances with foreign nations and to rely instead on temporary alliances for emergencies.
Foreign policy, he insisted, should be based on America's interests and none other. The address was a critical triumph and contributed mightily to Washington's legend. America would soon be without Washington, but thanks in part to his Farewell Address, it would not be without a guidepost to the future.
George Washington's Farewell address, delivered on September 19, 1796, remains a a towering. James Madison and Alexander Hamilton collaborated with Washington in penning his final. Unsettled Domestic Issues, a. domestic and the other foreign, which in the mid-1790s increasingly seemed likely to combine.
In his farewell address to the nation, George Washington cautioned Americans against "the. It also, he continued, "opens the door to foreign influence and corruption." Wise and timely words from.
Nov 13, 2013. george washington's foreign and domestic policies Essay. Washington, given his opinion on foreign affairs, felt that they should not have.
George Washington could not have envisioned the modern world, where technologies have forced once distant peoples to intermingle, but his original warning of foreign entanglements should still be.
The French Revolutionary Wars was the major issue during this period, especially the war between Britain and France. Washington tried to remain neutral, but.
The funds China steers toward its soft power campaign are hard to pinpoint due to the country's limited transparency but experts place estimates in the billions of dollars. U.S. sinologist David.
Washington was born at 10 AM on February 22, 1732 at the family estate on the banks of the Potomac River, in Westmoreland County, Virginia. Washington's father died when he was 11 years old. Lawrence Washington, George's older brother, apparently became a surrogate father for George.
President Washington bequeathed to future presidents a potentially powerful office, especially in the conduct of foreign affairs—and at the same time he set very high standards for the running of that office. In thinking about meeting challenges in life, there are few better role models than George Washington.
Document 15, President George Washington's Farewell Address,1796. Historical Background. George Washington's first term in office was marked by broad political support and the virtual absence of.
Thomas Yazdgerdi, special envoy for Holocaust issues at the State Department. Palij declined to comment when a Washington Post reporter went to his home earlier this month. In 2003, Palij told the.
George Washington's Foreign Policy. George Washington Foreign Policy. 19,516. views. Kelly Lucot. 4 uploads. Recommended.
Sep 24, 2010 · Further, a key player in the Revolution, Marquis de Lafayette, was one of George Washington's closest friends. Right now when composing this post, I've yet to read the following article from JSTOR entitled George Washington and the French Revolution.
Jan 11, 2017. 9 foreign policy issues the Trump administration will have to face. "good war," it was President George W. Bush's counteroffensive to the Sept.
Guidance for locating NARA records dealing with foreign affairs and diplomacy. Hosted by the George Washington University's Gelman Library, the National.
Briefly summarize George Washington's beliefs about political parties. 2. What warning about foreign nations does Washington give in his farewell address? 3. Why do you think Washington was so concerned about these two issues? 4. Evaluate the role of political parties in today's national scene. Were Washington's concerns valid for the future of.
Although Washington tended to side with the Federalists on most of the major issues during his presidency, he refused to tie himself to them, as he believed that the creation of political parties was a divisive step for the American government and people.
But in Washington, Ortagus has been a fixture of the GOP. "Morgan has been demonstrating the capability to deal with.
Instead of appealing to George Washington, Citizen Genet spoke directly to the people. His offensive behavior caused George Washington to ask for a new.


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More Thrift Store Finds.February 17th, 2010 in member junk
smiller0529, member
My thrift store finds. Thought the 3 plates would make a beautiful 3 tier serving piece; just have to find the right items to go between them. The basket I will put some burlap in it and plant flowers for my front porch and the two other items I'm thinking I will glue together for either a candy dish or maybe for some pretty soaps or potpourri. The green vase we will have to wait and see. I'm sure I will come up with something. All this for less than $10.00.
Pattern or design used: My own design
posted in: member junk, tabletop, centerpiece, plant holder, basket, lazy susan
Comments (7)
JunknStuff writes: I've been wanting some glass plates like these...tahnks for reminding me! I saw a magazine article once that used these "flower-shaped glass plates" as actual flowers in a garden! They cut slits on one end of a 1/4"-1/2" piece of copper tubing, slipped the plate in the slit w/ some adhesive, then added a copper leaf to the copper "stem." They stood a grouping of them in the garden area, & I've always wanted those lil cuties! Posted: 11:02 am on September 1st
JunkMama1 writes: Beautiful! Posted: 11:10 pm on March 3rd
fellowjunker writes: Wonderful treasures!
xojanis Posted: 7:54 pm on February 21st
xojanis Posted: 7:54 pm on February 21st
ninnarae writes: Those plates will make a charming server! Always fun to go thrifting!! Posted: 6:50 pm on February 18th
alicemom writes: Fun finds. Will make pretty display!
smiles, alice Posted: 1:55 pm on February 18th
smiles, alice Posted: 1:55 pm on February 18th
RustyDiva writes: Very pretty prism colors coming through on the glass. You done good!! Posted: 11:14 am on February 18th
suewhitney writes: Great finds!!
Take care,
Sue Posted: 10:13 am on February 18th
Take care,
Sue Posted: 10:13 am on February 18th
You must be logged in to post comments. Click here to login.


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Over the past several months I have been kicking into high gear a dream of mine; to create a free, yep – totally free, automated estate planning platform for lawyers. Over the years, one thing after another has prevented this dream from moving forward but things have started to change. While a lot of work remains, New England Estate Planning (NEEP) will open for attorney registration on January 1, 2020! I hope the actual site will launch, even if in a beta format, in 6 to 12 months after January 1st.
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To learn more, please visit http://www.newenglandestateplanning.com. If you want to be reminded when registration begins, please email your name, bar number and contact information to firstname.lastname@example.com or firstname.lastname@example.com.


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Every year BenRiach's core range is supplemented with various finishes and single cask bottlings. The details of batch # 13 of the latter were revealed on August 19, 2016.
This time Billy Walker, the company master distiller, selected twelve casks including several interesting wood finishes. The oldest whisky in the batch, 40 years old, came from the cask filled in 1975, the youngest one, 16 years old, from the cask filled in 1999. Here we got triple distilled whisky from May 27, 1998 finished in ex-PX sherry puncheon no. 6401. In June 2016 the puncheon yielded 670 bottles of cask strength whisky (57.3% abv).
Producer's tasting notes:
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text: DSpace Collection: http://hdl.handle.net/123456789/109 2019-12-11T21:55:33Z A PHYTASE FROM RHIZOPUS OR YZA E: PRODUCTION, CHARACTERIZATION AND APPLICATION STUDIES http://hdl.handle.net/123456789/2026 Title: A PHYTASE FROM RHIZOPUS OR YZA E: PRODUCTION, CHARACTERIZATION AND APPLICATION STUDIES Authors: Rani, Richa Abstract: Phytase is an important enzyme in the food/feed industry. It catalyzes the hydrolysis of phytate, an antinutrient compound present in cereals and grains, thereby, releasing orthophosphate and myo-inosito1-6-phosphate with lower degrees of phosphorylation. Phytic acid is a strong chelator capable of complexing with a variety of metal ions under neutral and alkaline conditions, as well as with proteins and starch under acidic condition. Treatment with phytase increases not only the bioavailability of inorganic phosphorus but also the digestibility of proteins and the absorption of minerals from food/feed. The action of phytase also contributes towards reducing the pollution in surface and ground water caused by the phytate and phosphorus run-off from manure in intensive livestock regions. This thesis presents the studies of a phytase from Rhizopus sp. The focus has been directed towards identification of a potential phytase producing microorganism, biochemical characterization of phytase, and optimization of parameters for the production of phytase, large volume production studies and evaluation of phytase potential in terms of phytate degradation. A novel phytase from mesophilic fungal strain Rhizopus oryzae MTCC 1987 was identified. The phytase showed acid stable and pepsin resistant properties, with its optimum activity at 45°C and a dual pH profile at 1.5 and 5.5. Besides superior activity at physiological temperature (37-39°C), it demonstrated high thermostability with t1127 0°C of 8.25 h, and a broad substrate specificity. All the tested metal ions (at 5 mM) exerted significant stimulatory effect except Fee, Niz+ and Cue, however, the interesting feature of this phytase was insensitivity to heavy metal ions like Bat+ and Ag+. Phytase was purified by using fractional ammonium sulphate precipitation and subsequent ion-exchange and gel filtration chromatography. Molecular size determination was carried out by SDS-PAGE and Gel filtration, and it was found to be —34 kDa and —34-36 kDa, respectively. Zymogram analysis displayed a single enzyme responsible for the phytase activity corresponding to —34 kDa. Kinetic studies demonstrated a significant 1 ABSTRACT low K,,, value (2.42 x 10-4mM), high catalytic efficiency (kcat/Km) (2.38 x 106 M-1 s 1) and low activation energy value (28.99 kJ/mol). An uncompetitive inhibition was observed in presence of tested inhibitors (Fluoride > Phosphate > Vanadate). Present study investigated the feasibility of various agro-industrial residues as solid substrate for phytase production, followed by optimization of culture parameters (substrate particle size, inoculum age, inoculum size, effect of supplementation of various surfactants and metal ions) under solid state fermentation (SSF). Linseed oilcake (LOC) and wheat bran (WB) (1:1) was found to be the most effective substrate with maximum phytase activity (37.43 U/gds) under optimal culture conditions. Present study also includes the statistical optimization of culture variables for the production of phytase under SSF. The study also aimed at enhancing the phytase production using an improved strain of Rhizopus oryzae followed by statistical optimization of the medium components for submerged fermentation. Higher titer and higher productivity of phytase by improved strain can contribute to a significant reduction of the cost of phytase production from R. oryzae thereby, increasing its industrial utilization. The ability of this phytase to completely degrade the phytate to its lower derivative, inositol tris-phosphate, was assessed by an improved method of HPLC analysis. It demonstrated the phytate degrading potency of this phytase using simple, precise and reproducible method using reversed-phase high performance liquid chromatography (RP-HPLC), equipped with variable wavelength detector (VWD). The feasibility of determining phytic acid and IP3 using this method was successfully demonstrated with minimal sample preparation and lower retention time at 246 nm. The results show that VWD system with UV spectrum specifically detects the phytate and its lower derivatives with a good resolution. Furthermore, an in vitro study in milieu of physiological conditions revealed an unusual stability of this phytase at low pH with a degradation of 50% of phytate content. In other way, it can be stated that even under low pH condition (and at physiological temperature), this phytase has the ability to break three phosphomonoester bonds of phytate. These findings, taken together, demonstrate the new enzyme from R. oryzae as a novel phytase and render it of potential industrial interest. ii ABSTRACT A novel strain improvement procedure has also been developed to increase the yields of phytase production through adaptation of R. oryzae cells to subsequent heat and cold shock. The outcome suggests that an enhancement in phytase production by R. oryzae could be obtained by the combination of heat and cold treatment of spore suspension. The study was further extended to a comparative study on efficacy of wild and mutant strains pertaining to ash content and mineral extractability. The results confirm the effectiveness of this procedure as well as the enhanced efficacy of mutant strain in term of mineral extractability.... 2011-01-01T00:00:00Z STUDIES ON PROTEASE INHIBITOR, ITS COMPLEX WITH TRYPSIN AND CHILECTIN FROM Tamarindus indica http://hdl.handle.net/123456789/2023 Title: STUDIES ON PROTEASE INHIBITOR, ITS COMPLEX WITH TRYPSIN AND CHILECTIN FROM Tamarindus indica Authors: Narhari, Patil Dipak Abstract: Seeds of leguminous plants are known to contain proteins that are important for a number of biological processes. Proteinase inhibitors, chitinases, lectins, globulins etc. from these seeds are the most studied ones, since Leguminosae family members are recognized as an excellent source of these biologically significant proteins. The Kunitz type inhibitors of serine proteases form an important field of scientific investigation due to their multifunctional abilities. The Kuntz STI inhibitors are included as Kunitz-P inhibitors in the I3A family of the MEROPS database (http://merops.sanger.ac.uk). Specific inhibitors are reported as insecticidal, anti-inflammatory, anti-cancer, anticoagulant, antiviral, antiparasitic etc. These multifunctional plant Kuntz type inhibitors are crucial tools enabling us to gain knowledge of the basic principles of protein interactions and could be important for human beings in control of many diseases by using them to discover highly potential drugs. So, it is necessary to search for novel Kunitz inhibitors with important functions. Applications of these types of multifunctional inhibitors are very extensive which give an inspiration to perform their structure based study to correlate their-structure with their activities. The structural properties of inhibitors that control the activity of the proteinases could be targeted as potential pharmaceutical products. Plant lectins are heterogeneous and highly diverse class of non-immune origin (glyco) proteins because of their carbohydrate-binding specificity, differences in molecular structure and biochemical properties. They are classified into twelve diverse families of evolutionary and structurally related lectin domains. There is no precise description of the biological functions of plant lectins because of their diverse classes and carbohydrate specificities. Several functions of specific 'lectins are reported which include antifungal, insecticidal, antiviral, antiproliferative, apoptosis-inducing and symbiosis mediating between nitrogen fixing microorganisms and legume plants. Lectins show extensive structural diversity and considering the structural folds, plant lectins are grouped into seven folds. Previous reports showed that plant lectins are also members of chitinase family in fold while they might possess or lack chitinase activity. To gain further insight into structure-function relationship of lectins in general, it is necessary to carry out structural study of these types of proteins which will also lead to understand their evolution and how they acquire new functions using the same parental fold. My thesis encompasses the cDNA cloning, isolation, purification, characterization, crystallization and structural studies of a Kunitz type inhibitor, its complex with trypsin and purification, biochemical and structural studies . of chitinase..~ike lectin (chilectin) from Tamarindus indica. The first chapter gives a general introduction and a brief review of the work done nationally and internationally for Kunitz type inhibitors and lectins. The second chapter describes purification and biochemical characterization of a Kuntiz type inhibitor from seeds of a leguminous plant, Tamarindus indica named as TKI (Tamarind Kunitz type inhibitor). TKI was isolated using a series of columns, initially Affi-blue column followed by DEAE an anion exchange column. The approximate molecular mass of the TKI was determined to be 21 kDa from SDS-PAGE analysis. The protein yield was 0.6 mg per gram of seeds and consisted of a homogenous mixture. The trypsin inhibitory activity and N-terminal amino acid sequence analysis revealed the isolated protein to be a protease inhibitor belonging to Kunitz type inhibitor family. The N-terminal amino acid sequence of TKI showed 79% homology with factor Xa inhibitor (BuXI) from Bauhinia ungulata and 64% with Trypsin inhibitor (BvTI) from Bauhinia variegata. TKI was tested for its anticoagulant, factor Xa inhibitory and antiproliferative activities and was found to possess anticoagulant and FXa inhibitory activities. Furthermore it also showed dose dependent suppression of the cancer cell growth of MCF-7, HepG2 and PC-3 cells. The third chapter contains cDNA synthesis, cloning, expression and purification of TKI. Briefly, the total RNA was isolated using three month old seeds after flowering and cDNA was synthesized. It was further amplified and cloned into pGEM-T easy vector and sequenced. The cDNA fragment amplified with PCR was —700 bp in length consisting of 3' UTR region and poly (A+) tail. A 555 bp ORF obtained after sequencing of the TKI gene coded for a polypeptide of 185 amino acid residues with a calculated molecular mass of 20575 Da. Sequence analysis showed that TKI belongs to Kunitz type STI family, however, has distorted Kunitz signature signature sequence due to insertion of Asn15 in the signature motif. TKI has maintained two characteristics of Kunitz STI family such as having molecular weight —20-22 kDa and conserved two disulphide bonds. The full length TKI encoding gene was cloned into pET-28cTEV vector and expressed Ninto BL21- DE3 competent Escherichia coli cells which showed expression and solubility of TKI but with negligible trypsin inhibitory activity which might be due to improper folding and disulfide bonds formed. Formation of correct disulfide bonds and proper folding might be ii required for TKI to maintain its inhibitory activity, so we expressed TKI in chemically competent E. coil K12 cells shuffle T7 which is engineered to form disulfide bonded proteins in -- the cytoplasm. SDS-PAGE analysis showed that rTKI was expressed as a soluble protein which has potent trypsin inhibitory activity like native TKI. Recombinant TKI was purified using Ni-NTA column and pure rTKI exhibited trypsin inhibitory activity. This result shows that rTKI is a stable protein and able to preserve its functional property and can be used further for biochemical studies. The fourth chapter encloses the structure of TKI, its complex with trypsin and molecular docking studies with factor Xa. Crystal structure of free TKI was solved at 1.9A showing Ji-trefoil fold consisting of 12 anti-parallel (3-strands with two disulphide bridges and an exposed reactive site loop having Arg at P1. To understand the mode of interaction of TKI with trypsin, crystal structure of TKT:pancreatic porcine trypsin (PPT) was determined at 2.2A. The stable complex formed by TKI blocked the Sl pocket of PPT by its exposed reactive site and-stabilized the complex by making interaction with the sub sites of PPT. The electron density for Asnl 5 was clearly visible in both free as well as TKI:PPT complex structure. In order to analyze the possible binding of TKI with FXa, molecular docking was performed. TKI Pl and P3 residues showed the interactions with Si and S4 pocket of FXa, respectively in a classical L-shaped substrate like conformation. Arg64, which. interacts with cation hole with its side chain occupying the aryl binding site of S4, is the most important residue of TKI which may provide specificity towards FXa. The Asn15 is important residue insertion in TKI which is involved in recognition and stabilization of complexes. Asnl5 interacts with four residues of PPT and two residues-of FXa and participates in stabilization of complex. Furthermore, it interacts with those residues which are important for specificity of FXa which point towards its role in recognition of specific proteases. Comparison of the reactive site of TKI with Kunitz STI inhibitors and factor Xa inhibitors show that it has distinct reactive site residues which possibly inhibit other trypsin like serine proteases and could play multifunctional roles. The fifth chapter encompasses purification, characterization, crystallization and structure determination of the chitinase-dike lectin (TCLL) and its complex with G1cNAc. The TCLL was purified using Affi-blue and DEAE column chromatography was found to be monomeric 34 kDa protein which shows negligible chitinase activity. The structure was determined at 1.49A and shows ((3a)8 barrel topology. The sequence of TCLL was proposed from crystallographic data and by mass spectroscopy. TCLL showed significant sequence similarity to the reported class III chitinase of GH18 family members. Evaluation of active site and substrate binding subsites revealed that it has mutations of key residues which are required for chitinase activity. Remarkably, the complex structure of TCLL with G1cNAc reveals that the latter binds in a pocket formed by two loops (34a4 and (35a5 which have different conformation in structures of GH1 8 members. From biochemical and structural studies, we concluded that TCLL is a chilectin which explored novel carbohydrate binding site other than chitintbinding groove in GH1 8 family members. However, TCLL does not fit anywhere into this classification of plant lectins by Van Damme et al.,(008[ Consequently, TCLL structure signifies a new class of plant lectins and confers evidence for evolutionary link of lectins With chitinases. The structure of TCLL also depicts how plants utilize the existing structural scaffolds ingenuously to attain new functions. So we propose a new class of lectin which is evolutionarily related to class III chitinase. Moreover, the one of the existing `Class V chitinase homologs' class of lectin and our proposed class `Class III chitinase homologs' have same fold. To avoid number of classes in lectin classification, we propose the new class "TIM barrel domain" rather than existing one and these two classes can be grouped into TIM barrel domain as one subclass. Furthermore, one cannot exclude the possibility that the glycosyl hydrolase family members or other ,families having TIM barrel topology from plants will not emerge as lectin. So the TIM barrel domain will be the main class and the forthcoming classes will be subclasses. 2012-01-01T00:00:00Z


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Before I went vegan I didn't really think much about what was in my protein powder. I only cared about getting enough protein after my workout. Then I read an article that discussed the amount of chemicals and toxins that are in many of the top selling powders, including the one used. Due to the inorganic ingredients the presence of pesticides is prevalent in many of these supplements. So, I transferred over to plant based protein powder. First I went with Vega protein and greens. Vega has two flavors vanilla and chocolate. Both are quite tasty. The vanilla is a great addition to a morning fruit smoothie as well. What I really like about vega is that you get 2 full servings of greens with your protein. Also there is no sugar added which is found in many of the non-vegan top selling proteins. Even with out the sugar I found Vega to taste better than my previous protein powder. As a student Vega can be a little pricey for me at times. So, I tried out Orgain Organic Protein plant based powder. So far I have only tried creamy chocolate fudge but they also have a vanilla flavor. It is cheaper than Vega, but for those saved dollars you sacrifice those servings of greens. Like Vega there is no added sugar and Orgain only contains organic ingredients so there are no remnants of pesticides. Since the only flavor I have tried is creamy chocolate fudge I have not put it in my fruit smoothies but sometimes I treat myself to a scoop of almond butter with my protein shake.
Vega Protein Powder: 29.2 Ounce, $47.30
Orgain Protein Powder: 32.48 Ounce, $22.50
Both of these protein powders I would recommend. Even though they are vegan they pack a large amount of quality protein in each serving. One thing I love to add to my protein shakes are chia seeds. The chia seeds create this goo substance that makes your protein shake have more of a milk-shake consistency. You just have to put it in the fridge for a little while after adding the seeds. So, I usually make it before my workout. I hope you enjoy either of these powders.
To see Majken's Protein Shake recipe click here!


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Jeringhave Hotels
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If you're looking for a place to get away, look no further than Jeringhave. Whether you're planning to stay for a night or for the week, the area around Jeringhave has accommodations to fit every need. Search for hotels in Jeringhave with Hotels.com by checking our online map. Our map displays the areas and neighborhoods around all Jeringhave hotels so you can see how close you are from landmarks and attractions, and then refine your search within the larger area. The best Jeringhave hotel deals are here with our lowest price guarantee.
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- • 5 5-star accommodations from SGD 137 per night
- • 73 4-star accommodations from SGD 91 per night
- • 107 3-star accommodations from SGD 78 per night
Things to See and Do in Jeringhave
What is there to see near Jeringhave:
- • Kurverwaltung Nordseebad Dangast (3.2 mi/5.2 km from the city center)
- • Kaiser Wilhelm Bridge (7.3 mi/11.7 km from the city center)
- • Jade Bay (7.4 mi/11.9 km from the city center)
- • Dangast Beach (3.1 mi/5.1 km from the city center)
- • Naturfreibad Zetel (3.4 mi/5.5 km from the city center)
When is the best time to visit Jeringhave?
- • Hottest months: July, August, June, September (average 69°F)
- • Coldest months: January, February, December, March (average 30°F)
- • Rainiest months: September, August, October, and July (average 3.49 inches of rainfall)


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Dandelion Dreams 2
Marcie A. Fowler September 14, 2004I know why . . .cause you do it so well!! Simply beautiful Julie. I love the effects and presentation of this image!! #168713
Julie Abston September 14, 2004Mystical and delicate - very nice, Julie!
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Julie Abston September 14, 2004Thank you so much Marcie and Bonnie!! I appreciate your comments!! #777748
Tammy L. Odell September 14, 2004Fantastic shot Julie!!Great job on the effects, they suit this shot well!! #777914
Julie Abston September 14, 2004Thank you Tammy!! I appreciate you taking the time to comment!!! #778445
Sharon Day September 14, 2004Fantastic shot, Julie!!! It's a lot of fun taking photos of the dandelions and goat beard seeds! #778891
Michael Allen September 14, 2004Julie, I love the delicate touch here.
Color, contrast and texture ~ they're all in such a nice harmony.
Very well seen and executed. Great framing.
..mike :-] #779134
Julie Abston September 14, 2004Thank you very much Sharon and Michael!! Your kind comments are very much appreciated!!!!! #780991
Joannie Bertucci September 14, 2004Champagne wishes and Dandelion dreams!
Julie Abston September 15, 2004Thank you for your extremely kind comments!! I really, truly appreciate it!!! #782136
Heather L. Jacks September 15, 2004Beautiful result! #783429
Julie Abston September 15, 2004Thank you so much Heather! I'm glad you like it and I really appreciate you commenting!!! #783724
Kevin Nugent September 17, 2004Beautiful image! I love the texture! #788153
Julie Abston September 17, 2004Thank you Kevin!! I really appreciate you taking the time to comment!! #788200
Michael Khoury September 22, 2004...very beautiful...artistic... #796711
Julie Abston September 22, 2004Thanks so much Michael! Your comment is very much appreciated!!! #797368
Susana Matos September 26, 2004superbe!
Terry R. Hatfield October 15, 2004Congratulations To You Julie!! #844768
Julie Abston October 15, 2004Thank you so much Terry!!! I appreciate it!! #845085
Diane Dupuis October 15, 2004Julie - this is fantastic! COngrats! #845684
Sharon Day October 15, 2004Congratulations, Julie!!! #845730
Julie Abston October 15, 2004Thank you so much Diane and Sharon!! I appreciate you both taking the time to comment!!! #846052
Debra Booth October 16, 2004Gorgeous image, Julie! Congratulations!! #847528
Julie Abston October 16, 2004Thank you so much Debra!! I appreciate it! #847562
Kathleen K. Parker October 16, 2004" I don't know why, but I have fun playing with dandelions!!!"
John P. Roberts, Jr. October 16, 2004I had a feeling this would be a winner. Beautiful use of color, and such a delicate composition. #849504
Julie Abston October 16, 2004Thank you Kathleen and John!! I truly appreciate you both commenting!!!! #849625
Larry Lawhead October 16, 2004Congratulations on making the finals, Julie! This is such a cool shot!!
cj patterson October 18, 2004Big Congratulations Julie!!!!! #853808
Terry R. Hatfield October 18, 2004Congratulations On Your Win Julie!!! #853825
Janet Detota October 18, 2004Congratulations, Julie! I love this one! #853993
Colette M. Metcalf October 18, 2004Congratulations to you on your win, Julie!!! #854004
Julie Abston October 18, 2004Thank you Larry, Carol, Terry, Janet and Colette!!! I appreciate it!!!!! #854070
Sharon Day October 18, 2004Congratulations on your win, Julie!!! I love this one! #854398
Stephen Zacker October 18, 2004Congratulations, Julie! #855037
Julie Abston October 18, 2004Thank you so much Sharon and Stephen!!! #855115
Wendy M. Amdahl October 18, 2004Congrats to you Julie! #855424
Debra Booth October 19, 2004Congratulations, Julie!! #855918
Julie Abston October 19, 2004Thanks so much Wendy and Debra!!! #856104
Donna L. Cuic October 19, 2004Nice work Julia. Congrats on your win.
Julie Abston October 19, 2004Thank you very much Donna! I appreciate it!!! #857777
Leonard Pierce October 21, 2004Congratulations on your Second Place win Julie!!! #861856
Diane Dupuis October 24, 2004Congrats Julie! Very well done! #867159
Julie Abston October 24, 2004Thanks so much Len and Diane!!!! #867178
Gradient overlay layer, texture and watercolor applied in PS7.
I don't know why, but I have fun playing with dandelions!!!
Uploaded on September 14, 2004
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