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Being celiac means always checking labels, it just becomes the normal thing to do. Of course there are just some things that you know are off limits, like normal crisps, so you just don't even bother checking them – until you do!
So, in the course of my days work, part of which is stacking shelves, I get started on the crisp section. Getting bored half an hour in I happen to glance at the ingredients of the crisp bag in my hand.
Nope, need to read that one again cos I didn't see the gluten filled bad boys listed.
Oh my god, these ones are gluten free!
OH MY GOD these ones are gluten free!!
Oh crap now I know these ones are gluten free.
I can't just unknow that. I can't take it back. I can stuff my face with crisps that I LOVE or I can pretend. Pretend I didn't just find this out, pretend that these crisps are still in the 'I can never eat those again' section of my diet.
What to do? ?
I'll just finish the bag I'm eating while I contemplate the answer. Could take some time, I might even need to make my way through a second…..


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Light Bulb Recycling
CFLs are a great energy saver, with one bulb lasting up to 10 times longer than an incandescent bulb! Because CFLs contain mercury, they are hazardous when broken. Therefore, it is especially important to not throw them away when they are at the end of their lifetime. Take them to 125 Bryan Building, or 117 Peabody Maintenance and they will be recycled for you (map of locations here). Due to concerns regarding breakage, do not send them in campus mail! Please hand-deliver. Place the CFL in a sealed plastic bag or a Ziploc-type baggie prior to coming to the drop-off location in the event that the bulb is dropped during delivery.
Small light bulbs can be picked up with waste via Vanderbilt's Waste Collection Form through the normal waste collection process.

subset: commoncrawl


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David Wilcock finally brings forward one of his top sources of insider information, Pete Peterson. In this exclusive one-on-one interview (Corey returns next week), David coaxes intricate details from Pete about his extraordinary life. From his days as a child prodigy to reverse engineering extraterrestrial technology, Pete Peterson has been inside secret programs for most of his life. Now you have the rare opportunity to gain a glimpse of what it is like to be on the inside. This interview with David Wilcock was originally webcast on December 13, 2016.
Subtitles available in English, Deutsch, Português, 中文, Русский, and Español.


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S: Francie Says...
BC: Francie wonders... The BIG question is... What will HENRY say?
FC: Francie Says | a collection of quotes by Francesca Pontius
1: lake and francie | Daddy: "" Goodnight, Francie. You're my little sweetie. FP: ""Goodnight daddy. You're my big boy.
2: Grammy Judy asks Francie to help unload the dishwasher. Francie sees a chance to unload in a different way than mommy says. She says: I'll get the sharp and fragile things.
3: One day, FP saw a beautiful rose in Judy's garden and wanted to know its name. Judy said it was named the Rose of Sharron. | Francie says, It's the Rose of Sharing
4: Giant has tea in Munchkinland
5: Mommy moves the juice bottle off the table. Francie says, Honey, please don't do that while I'm trying to read it.
6: doggies and little brothers | are a girl's best friends! | One thing's for sure... | d
7: Cousin Luke never makes mischief | Francie was making mischief of one kind or another one day (or maybe it was Henry). Mommy scolded FP, just a little. Francie said, | I don't like it when you talk to me that way.
8: More Doggies... " | Dr. and Waitress Francie says: Louis, close your eyes and open your mouth, please. | Louis says: What the...!
9: Henry says: What the...! | More Brother...
10: More Tea... with Dwarfs | guest appearance by Snow White
11: There is an Illinois Prairie Path that FP rides on, with mommy and Henry. Francie says: Let's ride the Fairy path
12: Daddy's home from work. He's going to play basketball with Francie. Francie runs to the house, saying: I'm playing basketball with Daddy. I have to put on my headband and bracelet.


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Get Free Rate Quotes.
Need Car Insurance Rates today?! Start here to get fast and easy comparative rates. For Home,Business or other types of Insurance please see our 'Request a Quote' window on our home page.
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Shameless Santa Christmas For Two
To Øl
Insufficient stock
Gift the gift of a good beer shared! 75cl bottle of Shameless Santa with the limited edition 2018 To Øl Tekus in Gold & Silver.
N.B. Teku Glasses are 0.3l Rastal Glassware. Bundle contains a set of 1 x Gold and 1 x Silver Teku Glass.


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Click on an alumni listing from South San Francisco, CA to find more information about the person.
The listing of alumni from South San Francisco, CA includes graduates from 4 schools. To filter results, select a South San Francisco, CA high school using the links to the right. The South San Francisco alumni list on this page come from a variety of sources. Clicking one of your friends will give you locations that you can get back in touch with the fellow graduate. To view alumni that graduated in South San Francisco around your class year, enter your graduation year in the search box at the top of the page. You may also be able to find friends close to your home town. We also have listings for graduates from San Bruno, CA, Millbrae, CA, or Daly City, CA.
Approximately 4,000 of residents in South San Francisco (6%) are the normal age for attending high school (15-19). Also, around 1,600 South San Francisco residents (2%) are the average age for high school seniors (18-19). Population stats are from the United States Census Bureau.
|Sign up for free to get in touch with high school friends
825 Southwood Dr
South San Francisco, CA 94080
South San Francisco, CA 94080
South San Francisco, CA 94080
|< 0
|over 85 >


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Justin + Allison | River House Events
Justin and Allison rented a house by the inlet waterway for their first look and getting ready photos and as you can see from Justin's awesome ring, there were a couple of avid fishermen that loved that spot as well. This was a beautiful DIY wedding and I hope you enjoy looking through every gorgeous detail of this couple's wedding day. The River House has a book nook where Allison pulled me aside and asked if I could photograph her and her grandmother 'reading a book' since that's what she did at her grandmother's house when she was younger. That was such a special moment. Congratulations again Justin and Allison!
Venue: River House Events
Hair/Makeup: CHS Makeup and Carly Sims
Florist: Jade Violet
Cake: The Pastry Studio
Catering: Designed Events
Tags: "natural light", april, d750, destination wedding, nikon, northeast florida wedding, passwater wedding, river house wedding, St. Augustine wedding photographer, stafl, vsco, wedding photographer


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Continued approval may be contingent upon verification of clinical benefit in a confirmatory trial The Food and Drug Administration (FDA) has granted accelerated approval to Brukinsa (zanubrutinib; BeiGene USA) for the treatment of adult patients with... Continue ReadingFDA approves Calquence Treatment for Treatment of Mantle Cell Lymphoma (November 6, 2017)
The U.S. Food and Drug Administration has granted accelerated approval to Calquence (acalabrutinib) for the treatment of adults with mantle cell lymphoma who have received at least one prior therapy. Mantle cell lymphoma is a particularly aggressive... Continue ReadingNovartis CAR-T cell therapy CTL019 unanimously (10-0) recommended for approval by FDA advisory committee to treat pediatric, young adult r/r B-cell ALL (July 12, 2017)
Recommendation based on review of CTL019 r/r B-cell ALL development program, including the pivotal Phase II global ELIANA trial A Biologics License Application (BLA) for this indication is under FDA priority review; if approved, CTL019 could become... Continue ReadingKite Pharma Updates Results of CAR-T cell therapy in Aggressive Non-Hodgkin Lymphoma (May 4, 2017)
Results from the ZUMA-1 trial of axicabtagene ciloleucel (KTE-C19) in patients with chemorefractory aggressive non-Hodgkin lymphoma (NHL) were updated recently at the American Society of Hematology Annual Meeting in San Diego, California. Overall 76%... Continue ReadingOpdivo® Highly Effective for Some Patients with Lymphomas (August 16, 2016)
The immunotherapeutic agent, Opdivo® (nivolumab), appears to provide long-lasting anti-cancer activity among some patients who have received extensive prior therapy for both B- and T-cell lymphomas. These results were recently published in the Journal... Continue ReadingCAR Therapy Effective in Advanced Lymphoma (June 20, 2016)
According to results of several presentations at the 2016 annual meeting of the American Society of Clinical Oncology (ASCO), chimeric antigen receptor (CAR) therapies appear to have significant anti-cancer activity among patients with different types... Continue ReadingDefitelio® Approved for Life-Threatening Complication of Stem Cell Transplant (March 30, 2016)
The United States Food and Drug Administration (FDA) has approved Defitelio® (defibrotide sodium) for the treatment of hepatic veno-occlusive disease (VOD) following a stem cell transplant. Defitelio is the first approved treatment for severe hepatic... Continue ReadingEnd-of-Life Care Remains Aggressive, Despite Patient Wishes (September 16, 2013)
Although most patients with advanced cancer prefer hospice or palliative care that minimizes symptoms, many continue to receive aggressive treatment during their final weeks and months of life. In fact, elderly patients with advanced cancer often receive... Continue ReadingCeliac Disease Associated with Higher Risk of Lymphoma (September 4, 2013)
Individuals with celiac disease have an increased risk of lymphoma, particularly if they have a condition known as persistent villous atrophy, according to the results of a large cohort study published in the Annals of Internal Medicine.[1] Celiac disease... Continue ReadingFollowing Lifestyle Recommendations Reduces Risk of Cancer Death (May 15, 2013)
People who follow the diet and lifestyle recommendations laid out by the World Cancer Research Fund (WCRF) and the American Institute for Cancer Research (AICR) have a 34 percent reduced risk of dying from several diseases and specifically, a 20 percent... Continue Reading


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We take a holistic approach to farming, which means we value diversity in our environment. We use natural methods to raise animals on pasture, and grow a number of different vegetables and flowers in our cutting garden. Rotational grazing and growing allow us to nurture the soil and keep our farm healthy.
This year we will be raising meat chickens, pigs, cattle, and turkeys. We use rotational grazing with our chickens and steers, moving to fresh pasture every few weeks. Our broad breasted bronze turkeys , raised for Thanksgiving, have a large area for roaming. The pigs are also kept in a large outdoor area. We supplement feed for our animals with grain from Napolean Mill, except for the cattle which are grass fed. There are no hormones or antibiotics in our meat.
Our farm is located on 10 acres in the Grass Lake area, and we are lucky enough to have a barn and house on site. We love to experiment with growing different vegetables and flowers, while keeping large areas in pasture for grazing and haymaking. The owners, Taik and Stephanie, have been farming for about 5 years.
We have a meat CSA that runs from June through November. See the CSA section for more details.
CSA stands for Community Supported Agriculture. CSA members pay upfront at the beginning of the year for a season's worth of meat. Throughout the year they receive meat packages at regular intervals. CSAs emphasize the community aspect of farming. There is a strong relationship between the farmer and the CSA members, who are closely involved in the process of producing their food.
We sell individual chickens or cuts of pork that can be ordered by contacting us directly. This summer you may also find us at Chelsea Farmers Market on Saturday mornings, and Howell Farmers Market on Sundays.


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Empire State Multi-District
President-Elect'sTraining Seminar
Empire State Multi-District
President-Elect'sTraining Seminar
Things you'll need to do:
1) Register! Registration link for the event via the "Registration Link" on the left side of this page. Please be sure to register. Districts 7120, 7150, 7170, and 7190 have arrangements with their clubs for one President-Elect for registration. If more than one PE (or future PE) wishes to attend, they should discuss with their District Governor-Elect. PE's from other Districts are welcome to attend Empire MD PETS, registration & meal costs will be provided upon request. Spouses and/or guests may also register to attend the Friday night dinner (dinner cost will be specified during registration).
2) Book Room! If traveling from outside the Syracuse area, you will want to stay over on Friday night March 13. For the Sheraton - our special room rate ($130 + tax) is also available on Thursday or Saturday night. You need to book your room separately from registering for the event. Room costs should be discussed with the PE's home club regarding reimbursement (if available). Please book your room by February 12 to make sure you get the discounted rate! Click here to book your room directly with Sheraton. This link INCLUDES the Empire PETS meeting discount code.
3) Plan! Work with your club, District Trainer, and District Governor-Elect and attend all President-Elect Training sessions that are held locally within your District. Attend other Rotary training sessions (Rotary Leadership Institute) and plan to be an active part of the District Training Assembly within your District. Start meeting and planning with your club's members that will be part of your "Board" during your term as resident Elect!
4) Get Excited! The District Governor-Elect's and the Empire MD PETS planning committee have arranged for some fantastic guest speakers and presenters. Your year of President will be one that you remember as a high point of your "Rotary Career". We look forward to your attending and participating at Empire MD PETS in March!
Sheraton Syracuse University Hotel & Conference Center
801 University Avenue
801 University Avenue
Syracuse, New York 13210
Program Highlights (from 2019 - check back for our 2020 program):
Friday, March 15, 2019
12 Noon - Registration Opens
12 Noon - Tech Lab Opens (optional)
12 Noon - Russell Hampton vendor Opens (Rotary merchandise)
1:00 pm - Community Service Event till 3:30pm (optional - details TBA)
4:00 pm - Empire PETS Training Begins
8:00 pm - Conclusion of dinner program - networking / social opportunities
Saturday, March 16, 2019
7:30 am - Breakfast opens
8:00 am - Empire PETS Training Resumes
3:45 pm - Empire PETS - District meetings (7120, 7150, 7170, 7190) - visiting PE's from other Districts welcome to attend as optional
4:45 pm - Conclusion of 2019 Empire PETS - enthusiastic participants depart Syracuse
(note: tentative/summary schedule, subject to change -- will be posting detailed schedule from Empire PETS program once finalized & sent for printing)
Service Project / Volunteer Event (Friday 1:00pm - 3:30pm):


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"We just talked about this in class"
"We are having a test on this next week"
"I just learned this in class"
So as you can see from all of the feedback from the students, this is def. an app that aligns with what they are learning in class! It is free for the first few levels, but once you see how excited the students get, you are going to want to purchase this app.
This week there is no Student Success Tip of the Week because I want to know what is your Student Success Tip? Leave your Student Success Tips in the Comments Section.
Happy First Day of Spring and Enjoy!


text: Dotta.io Description About Dotta.io New game Dotta.io is free online at our website Iogamescom.com. You can play this game in your browser, your mobile and enjoy it for free. Your task in this game to find different funny monsters. You must fight the monsters. You will fight them hand to hand. You will kill the monsters as many as possible to get high score and win. I hope that you will like this fighting game and come back to play others game. We update new game daily. Have fun! How To Play Dotta.io Use your mouse to move your dotta character.


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Quellen - Anwendungshinweise
Automated Imaging and Dual-Mask Analysis of γH2AX Foci to Determine DNA Damage on an Individual Cell BasisDownload
Related Products: Cytation 5 Multi-Detektions-Reader für Cell Imaging
August 11, 2016
Authors: Brad Larson, BioTek Instruments, Inc., Winooski, VT USA; Asha Sinha and Sachin Katyal, University of Manitoba, Winnipeg, Manitoba, Canada
In mammalian cells, one of the most serious types of DNA damage is a double-stranded break (DSB), where the DNA double helix is completely severed. DSBs are associated with cell death and tumorigenesis, so they are of keen interest in understanding cancerous mechanisms as well as developing therapeutic treatments. One of the most characterized early markers of DNA DSBs is the phosphorylation of the histone 2AX (H2AX) to γ-H2AX. Here, a positive feedback loop is created between γ-H2AX and phosphatidyl inositol 3' kinase-related kinases, where repair systems are recruited to the damage, creating nucleation sites, or foci, that correspond to the level of DSB. When the foci are bound to fluorescently labeled antibodies, overall γ-H2AX levels can be visualized and quantified via immunofluorescence microscopy.
The conventional, manual method of evaluating foci via a γ-H2AX assay offers limited throughput and is subject to operator variability. Incorporating automated imaging and analysis into the assay workflow can help to enhance throughput, assay robustness and accuracy, while also increasing overall laboratory efficiency. Additionally, while manual methods allow for overall population assessment captured within an image or set of images, they are not conducive to analysis of individual foci within the population.
Here, we demonstrate an automated γ-H2AX assay workflow using a novel cell imaging multi-mode reader with advanced data analysis capabilities to process and image multiple samples simultaneously, including dual-mask capabilities to assess real-time population-level data as well as individual foci data, for a complete data set. This automated assay format provides an accurate, robust method to assess DNA damage in mammalian cells.
Materials and Methods
U251 (human glial) cells (Catalog No. 09063001) were purchased from Sigma Aldrich (Saint Louis, MO).
Assay and Experimental Components
The known cytotoxin, camptothecin (Catalog No. 208925) was purchased from EMD Millipore (Billerica, MA). The Alexa Fluor® 647 anti-H2A.X-Phosphorylated (Ser139) antibody (Catalog No. 613407) was purchased from BioLegend (San Diego, CA).
Cytation™ 5 Cell Imaging Multi-Mode Reader
Cytation 5 is a modular multi-mode microplate reader combined with automated digital microscopy. Filter- and monochromator-based microplate reading are available, along with laser-based excitation for Alpha assays. The microscopy module provides up to 60x magnification in fluorescence, brightfield, color brightfield and phase contrast. With special emphasis on live-cell assays, Cytation 5 features shaking, temperature control to 65 °C, CO2/O2 gas control and dual injectors for kinetic assays. The instrument was used to image the stained DNA using the GFP imaging channel. Integrated Gen5™ Microplate Reader and Imager Software controls Cytation 5, and also automates image capture, analysis and processing.
Gamma H2AX Assay Performance
Cultured U251 cells were treated with concentrations of camptothecin ranging from 1-0 μM. Immunostaining was then performed using the parameters listed in Table 1.
Table 1. Immunostaining procedure using Alexa Fluor® 647 anti-H2AX-Phosphorylated (Ser139) antibody.
Following completion of the staining procedure, the plate was then imaged by the Cytation 5. Table 2 lists the settings used to perform automated image capture of each sample well.
Table 2. Automated gamma H2AX imaging parameters.
Image Pre-Processing
Image pre-processing was applied to remove excessive background signal from the images using the criteria listed in Table 3. As the individual foci are small in diameter, and may be in close proximity within the nucleus, an optimized subset, or area, of the image that is analyzed to distinguish between background and true signal was required. A greatly reduced value of 2 μm provided the most optimal final images for subsequent analysis.
Table 3. Image pre-processing parameters.
Primary Foci Analysis
Primary mask cellular analysis criteria (Table 4) were applied to automatically place object masks around individual foci. The average area of an individual foci was then used in conjunction with results from the dual-mask analysis to calculate the number of foci per nuclei on an individual foci basis.
Table 4. Foci analysis parameters.
Dual Mask Individual Nuclei Foci Analysis
Primary mask cellular analysis criteria (Table 5) were applied to automatically place object masks around nuclei in each captured image. Secondary mask cellular analysis criteria (Table 6) were then also applied to place linked additional masks around individual foci. The area of the primary mask was minimally expanded such that areas within the primary mask meeting the criteria to identify foci would still fall within the nucleus. "Threshold in Mask" was also selected to allow individual secondary masks to be placed anywhere within the initial primary mask meeting the included criteria.
Table 5. Primary mask nuclei analysis parameters.
Table 6. Secondary mask foci analysis parameters.
Results and Discussion
Automated 96-Well γH2AX Assay Imaging and Pre-Processing
Automated γ-H2AX assay imaging, performed in 96-well format, was validated using U251 cells exposed to various camptothecin concentrations. Cytation 5 automatically imaged the samples using a 3x3 image montage to ensure that a statistically relevant number of nuclei were analyzed per image. Auto exposure was used with each imaging channel (Table 2) and optimized using positive and negative control wells to ensure that the integrated Gen5 Microplate Reader and Imager Software settings accurately imaged the nuclei and labeled foci in each image.
Following image capture, Gen5 automatically pre-processed the samples to remove excess background signal and properly prepare each image for analysis. In the case of assays where the signal of interest emanates from small punctate areas within the well, this step is critical, particularly when large numbers of puncta fall within small areas of the image. This is observed in Figure 1A where cells were exposed to a high (1 μM) concentration of camptothecin. While individual labeled foci can be seen in certain nuclei, others containing higher foci numbers appear where the nuclei is completely covered with the fluorescent signal. This phenomenon precludes the possibility of performing accurate analysis of the DNA damage within these nuclei.
Figure 1. Image background signal removal via pre-processing. Zoomed 10x images (A) before; and (B) after pre-processing criteria (Table 3) applied using Gen5 Software.
By performing the pre-processing step in Gen5, with the incorporation of a 2 μm diameter in which to analyze each portion of the image, superfluous fluorescent signal not associated with the labeled foci is eliminated from the image (Figure 1B). Individual labeled foci are then visualized in each nuclei and accurately analyzed.
Following image capture Gen5 automatically stitched together the nine individual image tiles in the original configuration of the 3x3 montage. This allows for analysis of the entire imaged area of the well.
Figure 2. Image stitching. Final stitched image of the nine tiles included in the 3x3 montage.
The final images accurately portrayed the extent of DNA damage from each test condition, where low numbers of labeled foci per nuclei were seen in untreated cells (Figure 3A), while those treated with camptothecin showed increasing numbers of nuclei affected as well as an increasing number of labeled foci per nuclei (Figures 3B-D).
Figure 3. Final processed images following camptothecin treatment and immunofluorescent staining. Zoomed images captured using a 10x objective and 3x3 montage of U251 cells exposed to (A) 0 μM (untreated) camptothecin; (B) 0.01 μM camptothecin; (C) 0.1 μM camptothecin; or (D) 1 μM camptothecin. Blue: Hoechst 33342 stained nuclei; Red: CY5 signal from labeled foci.
Automated γH2AX Assay Analysis
Individual Foci Identification
Primary cellular analysis criteria (Table 4) were applied to all images captured with Gen5 Software using the fluorescent signal from the CY5 channel to place object masks around individual labeled foci (Figure 4). Minimum and maximum object sizes were set low due to the small size of each labeled foci. The background flattening size, or rolling ball diameter, was also set to 2 μm as was seen in the image pre-processing step, to aid in accurate identification of each foci. The area of each individual foci was reported and averaged per image. A value of 1.95 μm was determined as the average area of a labeled foci. This value was then used in the second cellular analysis step to calculate the number of labeled foci within each individual nuclei.
Figure 4. Automated cellular analysis for specific identification of labeled foci. Object masks placed using signal from antibody labeled foci. Images captured using a 10x objective, 3x3 image montage, and DAPI and CY5 imaging channels.
Dual-Mask Identification of Labeled Foci per Nuclei
Primary and secondary cellular analysis criteria (Tables 5 and 6) were applied to all images captured using Gen5. Primary cellular analysis automatically masked each nuclei (Figure 5A), while secondary analysis automatically placed individual masks around nuclear foci specifically within the original primary masks (Figure 5B).
Figure 5. Automated γH2AX dual-mask analysis based on user-programmed parameters. Primary cellular analysis object masks showing (A) nuclei and secondary masks showing (B) labeled foci, respectively. Images captured using a 10x objective, 3x3 image montage, in addition to DAPI and CY5 imaging channels.
By using primary cellular analysis to initially mask nuclei, the foci identified were properly linked to each nuclei; allowing for analysis on a single nuclei level.
Subpopulation analysis was then applied to identify nuclei in each image exhibiting positive DNA damage. The total signal from the CY5 channel within objects identified using secondary analysis was used to apply threshold criteria. As foci appear and increase in number per nuclei the total fluorescence in the CY5 channel within the object masks placed around the foci also increases. Therefore, a value greater than 100,000 RFU was set to eliminate nuclei exhibiting low levels of fluorescence in the CY5 channel not emanating from true labeled foci. Using this criteria, nuclei containing distinct labeled foci meet the set threshold parameter for being γH2AX positive cells (Figure 6).
Figure 6. γH2AX positive nuclei. (A) Nuclei meeting γH2AX positive subpopulation criteria; and (B) labeled foci within positive nuclei. Positive γH2AX nuclei in purple and negative nuclei in yellow.
Labeled Foci per Individual Nuclei Calculation
Upon completion of the gH2AX assay procedure and image preparation, Gen5 was used to calculate the commonly used assay metric "labeled foci number per nuclei" on an individual nuclei basis. This was generated using the following formula and object metric of interest (Table 7), in addition to the average foci area value from the initial foci analysis (1.95 μm2), to automatically calculate the number of foci per nuclei:
Table 7. Number of foci per nuclei metrics.
When the total area is divided by the average area of a labeled foci, the final foci count within nuclei is yielded.
Dual-Mask γH2AX Assay Validation Data
The results in Figure 7A illustrate that the total coverage area per nuclei of labeled foci increases in direct correlation to the camptothecin treatment level. Then by applying the formula explained above, the number of labeled foci per nuclei is calculated (Figure 7B). This value agrees with expected results as the number of foci in an individual nucleus starts at an insignificant level in wells containing untreated cells, indicating little to no DNA damage, and increases directly with camptothecin treatment.
Figure 7. Labeled foci analyses using U251 cells exposed to various camptothecin concentrations. (A) Total foci coverage area per nuclei, and (B) calculated foci number in individual nuclei. Twelve replicates tested at each compound concentration.
Because the foci count is calculated from the total foci coverage area for each nuclei counted in the image, using the dual-mask analysis, it is also possible to quickly assess results for an individual nuclei by clicking on that object. The linked data is then highlighted (Figure 8). Therefore, if an interesting phenomenon is observed in a particular object, all relevant data can be immediately scrutinized.
Figure 8. Observation of single object data. Objects of interest highlighted in red. Relevant data such as labeled foci area coverage and calculated foci count highlighted in the data table to the right.
Finally, using the data from the dual mask individual nuclei foci analysis, subpopulation parameters were applied to the primary objects, representing txd in each image using the criteria listed in Table 8. The criteria used was the combined total signal within object masks placed around foci interior to previously identified nuclei. The established condition separates the nuclei exhibiting DNA damage, as witnessed by a minimal total signal from identified labeled foci, from undamaged nuclei.
Table 8. Gamma H2AX positive cell criteria.
Using the subpopulation parameter listed in Table 8, the number of gH2AX positive nuclei per test condition was also automatically determined (Figure 9A). By comparing this number to the total nuclei in the image, a normalized gH2AX positive nuclei percentage was then calculated (Figure 9B).
Figure 9. γH2AX positive (A) nuclei count and (B) ratio to total nuclei counted. U251 cells exposed to various concentrations of camptothecin. Twelve replicates tested at each compound concentration.
From the graphs in Figure 9 it is also evident that the number and ratio of nuclei meeting the established subpopulation criteria increase when cells are exposed to higher concentrations of camptothecin.
Results for both foci count per nuclei and percent γH2AX positive nuclei exhibit similar trends across all camptothecin concentrations tested, confirming the ability of the automated imaging procedure and dual-mask analysis method to deliver accurate, robust data on an individual nuclei and population basis.
Incorporating a cell imaging multi-mode reader to automate the γ-H2AX assay provides increased throughput to aid in laboratory efficiency. At the same time, dual-mask capabilities allow population level analyses as well as single nuclei scrutiny, and all analyses are automatically performed without the need for separate software. Automated imaging and analyses also eliminates human subjectivity and analysis errors that could potentially skew results. This creates a robust, user-friendly and accurate method to detect DNA damage in mammalian cells.


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It's always tough having to do a goal reset when you think you've got it all sewn up. Kudos to you for knowing when to walk away. That takes guts–exactly what you need in this business. Good for you. 🙂
"The greatest accomplishment is not in never falling, but in rising again after you fall." ~ Vince Lombardi
Almost a month ago, I included this quote in the post that announced the termination of my work with my publisher.
I never thought I'd have to announce that. As a matter of fact, I thought I would be pitching them series after series of work. I thought I'd found a writing home.
There were differences, creative and otherwise, and after a while, I said the words that I knew would allow me to walk away. This isn't a post about what went wrong. It's about how to keep going when your dream was almost accomplished, and then slipped through your fingers.
My first thought, when I accepted reality, was 'why does it always have to be another battle?'. I've battled a lot in my life. Battling with the mental illnesses of myself and others. Battling with abuse. Battling with people who refuse to believe they've done anything wrong. Battling with people who refuse to accept my truth as a version of their reality. Battling to protect my son. Battling through physical illnesses. Battling, battling, battling.
Nobody said it would be easy, did they?
My second thought was an effort to stop the endless parade of self-pity. My second thought was, 'what now?'
What now, indeed.
I turned to my manuscript and started reading. For the most part, after an unending amount of rewrites, I liked what I had in front of me. There were some things I would change. Some things I wished to return to their previous state. But my manuscript was better for the experience, so I took a deep breath and tried to look at the bright side. Maybe I could make headway now, where it was once impossible.
The truth is, I love my characters. I need to tell their story, and I want to live in their world. I'm connected to this. They feel like old friends. So, maybe I was too attached? Maybe I couldn't see my work clearly?
I sent a few chapters off to some writing contacts who could help me adequately judge where the story was going, to make sure I wasn't seeing my own work through rose-colored glasses. I wasn't. They were enjoying it, asking me for more chapters. I felt better.
When you've looked at the story as many times as I have looked at this one, when you've been given as many opinions about it as I have, you start to question your sanity. You think you've nailed something time and time again, but you're then told that you haven't, and eventually, you lose the ability to tell the difference between what is well written and what is a mess.
I'm learning to trust myself again. Learning to look at my work and go with my gut. But I can't just stop. It's not who am I. Because all of that battling I mentioned earlier? I complained about all of it. But I battled. And I won. I'm still here. The universe has been trying to buck me for awhile but it has failed. And as long as I'm here, I'm going to keep doing what I love.
I'm going to keep writing.
So, I'm reading through the book again, just to make sure it's the vision I want to portray. And then I've got someone I trust who is going to edit it. And then I will throw my book back out into the pool and try, Try, TRY to get another set of eyes that loves it like I do, that shares my vision. Because, that's where I struck out before.
I don't expect much. I'm fully accepting of the idea that I may end up choosing to self-publish it, in order to keep my vision alive. Or maybe my book will wind up in a better place than I ever imagined.
One thing is for sure. I have never given up on anything, and I won't give up on this.
Justine Manzano is a multi-genre writer living in Bronx, NY with her husband, son, and a cacophony of cats. She maintains a semi-monthly blog at JustineManzano.com and a twitter account@justine_manzano, where she discusses her adventures in juggling motherhood, writing, and the very serious businesses of fangirling and multiple forms of geekery. Her first novel, a YA Fantasy titled The Order of the Key, is currently searching for a caring home.


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I could share all of the health benefits of laughing, tell you stories about how laughter has been used throughout the ages, give you links to laughter yoga practices around the world…
Or we could just sit here together and LAUGH. Yeah, let's do that one.
Come on, let's see what it takes to get you giggling.
A few jokes to start:
Q: Why shouldn't you tell a secret around a clock?
A: Time will tell.
I was wondering why the ball kept getting bigger and bigger, and then it hit me.
Q. What do you call a fish with no eyes?
A: A fsh.
(Try telling this to someone out loud.)
Any snickers yet? Okay, maybe just a few groans.
How about some comics and an awesome site:
(I laugh every.single.time. I go to this site)
You have to be at least smiling now.
Let's get things really going – watch one or both of these:
(and a little clip from, "The Money Pit" – make sure to watch this until the end…)
There we are. Feel the belly laughter!
Pass this along…let's see if we can get the whole world laughing together.
About Lisa Renee Wilson
I am honored to be your host for this journey! I am an Awareness Artist who shares her world work through Being Breath (www.BeingBreath.com). As a parent, artist, contemplative, meditator, yoga practitioner, introvert, runner, blogger, photographer, and iced-chai addict, I practice the art of mindful awareness throughout all of my daily activities. From playing with perspectives to playing in paint, no moments are off limits for exploration.
You can learn more about me over at BeingBreath.com, connect over on Facebook, Pinterest, or Twitter, or just stay tuned to hear insightfully honest stories right here at Bliss Habits!


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Out of all the stories I've ever heard about the power of love between siblings, hearing about a sister who loves her brother so much, she's pregnant with his twins definitely takes the cake. Yes, I know that sounds pretty Jerry Springer-ish, but it's anything but scandalous, I assure you.
Tiffany Burke is pregnant with her brother James' twins because she agreed to serve as a surrogate to him and his wife, Natalie, since Natalie is no longer able to carry children on her own after losing her uterus in an emergency surgical procedure.
Wow, can you even imagine having a sister-in-law who loved you enough to carry your babies? Tiffany is one very unselfish soul for sure, especially considering just how sick and tired this pregnancy has made her.
She's suffered from horrible nausea for most of the pregnancy so far, which has made her resort to sleeping the days away for relief. And she's even found it hard to spend time and tend to her two own children, ages 7 and 3, because she's so sick and exhausted most of the time.
And even though being a surrogate is something she truly wanted to do for her brother, I can't even imagine how much guilt her sister-in-law Natalie must be feeling for putting such a strain on her life. It must be so tough for her to watch Tiffany struggling simply to manage her normal, everyday routine -- all while knowing she's carrying two babies who belong to her, who will be placed in her arms at the end of the pregnancy instead of Tiffany's.
While she's no doubt eternally grateful to her for being willing to give her and James the gift of more children, I can't help but wonder if she has days when digging out from under the guilt is almost unbearable. She must feel so indebted to Tiffany for the rest of her life, because there's just no way you can ever repay something like that.
And as much as people will applaud Tiffany for carrying these babies for her brother and sister-in-law, Natalie's strength should be noted as well. Not many women would be able to handle a journey like this without falling apart.
Would you ever let a family member be your surrogate?
Image via Crystal_1979/Flickr


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Cases Do Not Come With Guidebooks. You Need An Attorney Who Will Forge An individualized Path For You.
Divorce and family law matters are overwhelming, not only because of the emotions involved and the life changes that come along with them, but because of the sheer volume of issues that need to be addressed and the complex laws that come into play.
Unfortunately, there is no guidebook, because every case is different. Your situation is unique, and a one-size-fits-all approach would not be effective in helping you achieve your goals. You need a guide who will carefully review your circumstances, get to know you and your family, learn about your goals, and then forge a path through your case.


text: June 16, 2015 The 5-Year Plan for National Childhood Nutrition: Don't Undo the Progress Read the article on Dr. Sears Comments: Since most of the children are throwing out the currently provided food, perhaps a better use of tax payer dollars might be envisioned. Leave a Reply Cancel replyYour email address will not be published. Required fields are marked *CommentName * Email * Website < Prev | Next >


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I spy number 43!Other than bawling my eyes out at the end of last night's "Bad News" episode of How I Met Your Mother (I'll save you the spoiler until after the jump but oh.my.god.), the only other major thing that I took away from what should have been an amazing episode was a countdown.
Seriously, what was up with the numbers?
You may not have noticed it, but between the scenes of Lily and Marshall's visits to the Barney doppelganger/gynecologist and Robin starting her new job, there were carefully placed numbers counting down from 50-1. And it bugged the hell out of me!
It distracted from both the humor and the emotional impact of realistic issues that this show usually blends so perfectly together. Instead of worrying along with Lily and Marshall about their infertility issues, all I could see was a big fat 43.
**Spoiler Alert**
And if you saw the episode, you know what the numbers were ultimately counting down to -- Marshall's dad's death and perhaps one of the most heart-tugging moments on HIMYM to date. Still, I felt like this cutesy Easter Egg gimmick cheapened the emotional moment. It was a fun game up until WHAM! You're hit with that news.
Of course you can argue the deeper meanings behind it, like how you can count down scheduled events in life -- days until graduation, months of pregnancy -- but there is no way of knowing when the unscheduled, yet just as life-altering events will take place, or maybe it's a metaphor for how our life is limited. Whatever the reasoning, instead of paying attention to what was going on in the scenes, you found yourself looking around for the numbers. There were times when I actually rewound if I missed one (crap, where was #15?!). For an episode that had so much going on in it, this was not the ideal one to try this gimmick out on.
Did you watch last night's episode? Did you catch the countdown? If so, did it distract your from the episode?
Image via CBS.com


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BCS Meaning - What does BCS mean?
BCS meaning is Ballard County Schools and other full form of BCS definition take part in below table. There are 99 different meaning of BCS acronym in the table which are compilation of BCS abbreviation such as Kentucky, Maine, Usps, Postal, Us post etc. terminologies. Unless you can not find the meaning of BCS acronym which you look for in 99 different BCS meaning table, please search again as using question model like "What does BCS mean?, BCS meaning" or you can search by typing only BCS abbreviation in the search box.
Meaning of BCS acronyms are registered in different terminologies. Especially, if you wonder, all meanings belonging to BCS acronyms under a terminology, click related terminology button at the right side(bottom for mobile phones) and reach BCS meanings which recorded to only that terminology.
BCS Meaning
- Ballard County Schools Kentucky
- Bangor Christian School Maine
- Bar Code Sorter Usps, Postal, Us post
- Barclays, P. L. C. Nyse symbols
- BarCode Sorter postal service
- Bardeen Cooper Schriefer Electronics
- Bardeen Cooper Schrieffer theory of superconductivity Physics
- Bardeen-cooper-schrieffer Atmospheric Research
- Bardstown City Schools Kentucky
- Barren County Schools Kentucky
- Baseline Comparison System Military
- Baseline Configuration System General
- Basic Catalog Structure General
- Basic Combined Subset General
- Basic Content Services IT Terminology
- Batch Compiler Specification File File Extensions
- Bath Central School New York
- Bath County Schools Kentucky
- Battered Child Syndrome British medicine, Medicine for Children
- Baugo Community Schools Indiana
- Beam Control System NASA
- Bear Creek School Washington
- Beaufort County Schools North Carolina
- Bedford County Schools Tennessee
- Belen Consolidated Schools New Mexico
- Bell County Schools Kentucky
- Bellevue Christian School Washington
- Bellevue Community Schools Michigan
- Benton County Schools Tennessee
- Berkeley Chess School, Berkeley, California Chess
- Berlin Community School New Jersey
- Bertie County Schools North Carolina
- Bessemer City Schools Alabama
- Best Case Scenario General
- Best Choice Selected General
- Best Climb Speed Transportation
- Bethany Christian School Michigan, High school, School
- Bethel Christian School Louisiana, Pennsylvania, High school, School
- Bethesda Christian School Indiana
- Bethlehem Christian School Mississippi
- Better Chemical Solutions Rail transport
- Big Crappy System Software
- Big Crock of Stuff Chat
- Birmingham City Schools Alabama
- Blackford County Schools Indiana
- Blackhawk Christian School Indiana
- Bladen County Schools North Carolina
- Blair Community Schools Nebraska
- Blanchet Catholic School Oregon
- Bledsoe County Schools Tennessee
- Blood Cell Separator Blood Test
- Bloomington Christian School California
- Blount County Schools Tennessee
- Body Cavity Search Police
- Body Condition Scoring Medical
- Boeing Computer Services Military
- Boiler Control System Power Plant
- Boland Calibration Settings File File Extensions
- Boone Central Schools Nebraska
- Boone County Schools Kentucky
- Border Cargo Selectivity Transportation
- Border Control System Software
- Bosnian Croat And Serbian Regional
- Boston Computer Society General
- Bowl Championship Series Sport
- Bradenton Christian School Florida
- Bradford Central School New York
- Bradley County Schools Tennessee
- Bragg Crystal Spectrometer Astronomy, Physics
- brazosport christian school Texas
- Breast Cancer Screening Breast, British medicine
- Breast Cancer Survivor British medicine
- Breast Conservation Surgery Breast
- breast-conserving surgery British medicine
- Brewton City Schools Alabama
- Briarcrest Christian School Tennessee
- Briarwood Christian School Alabama
- Brisconnections Unit Trusts (Australian Securities Exchange [ASX]) Asx symbols
- Bristol City Schools Tennessee
- British Cardiac Society British medicine, Cardio
- British Chemical Standard Chemistry
- British Computer Society Telecom, General
- British Crime Survey Police, Uk police
- Broadband Cable System (UW) Science
- Broadcast Control Station Army & Military
- Brunswick County Schools North Carolina
- Budd-Chiari Syndrome British medicine
- Bumbling Crappy System Software
- Buncombe County Schools North Carolina
- Burke County Schools North Carolina
- Burner Control System Power Plant
- Business Case Study Business Word
- Business Communication Services SAP
- Business Computer System General
- Business Controlling System Software
- Butler County Schools Alabama, Kentucky
- Sonic Foundry Batch Converter Script File Extensions
- Southern Seaplane, Belle Chasse, United States Iata Airport Codes, United States
- Windows95 Browse information File Extensions
Please also find BCS meaning in other sources.
- Click and read BCS in Wikipedia.
- For find questions and answers, look at search results BCS in Yahoo Answers.
- And finally again and again search BCS in Google.
What does BCS stand for?
We have compiled queries on search engines about BCS acronym and we gave place them in our website by selecting the most frequently asked questions. We think you asked a similar question to the search engine to find meaning of the BCS abbreviation and we are sure the following list will take your attention.
What does BCS meaning stand for ?BCS meaning stands for Bradenton Christian School.
What is the meaning of BCS abbreviation ?The meaning of BCS abbreviation is "Broadband Cable System (UW)" .
What is BCS definition ?BCS definition is "Briarwood Christian School".
What does BCS mean ?BCS mean that "Sonic Foundry Batch Converter Script".
What is BCS acronym ?BCS acronym is "Breast Conservation Surgery".
What is shorthand of Bardstown City Schools ?The shorthand of "Bardstown City Schools" is BCS.
What is the definition of BCS acronym ?Definitions of BCS shorthand is "Boston Computer Society".
What is the full form of BCS abbreviation ?Full form of BCS abbreviation is "Bear Creek School".
What is the full meaning of BCS ?Full meaning of BCS is "Bethel Christian School".
What is the explanation for BCS ?Explanation for BCS is "Brewton City Schools".
What is the meaning of BCS Abbreviation in Astrology ?
We did not give any place only meanings of BCS definitions. Yes, we know that your main purpose is the explanation of BCS abbreviation. However, we thought, you can consider the astrological information of BCS acronym in Astrology. Therefore, astrologic description of each word are available at the bottom.
BCS Abbreviation in Astrology
- BCS (letter B)
Sensitive, fragile and withdrawn. When it is located as first letter in the name, it gives its all power to other letters. People whose their names start with B have strong intuition. Peace and consensus are only purpose for them. They want peace where they are stay on. These people are friendly, loving domestic life, sempatic and also laggard. However, what they do is reliable and robust.
People who has B as last letter in their names, finish their works late. But, they usually do the best. They are fond of beauties and being loved.
- BCS (letter C)
Have a finger in every pie, Low concentration, diffident, vivacious and cheerful people. People whose their names start with C are creative, amusing and have strong communication skills. Especially, their hand skills are improved.
C resembles semicircle and these people feel themselves deficient. Change is inevitable for them. Being impatient is their most insufficiency for them. People who has C as last letter in their names are slog on what they do since they get bored quickly. Not to finish a job is so common.
- BCS (letter S)
The S letter person which managed by Saturn, is known for his strong and study . He does not hesitate to fight and indomitable in the face of difficulties. He makes a difference at every job since he is productive. He wants to be known by the public, it wants to be recognized.
Their charismatic structure and friendly behaviors make them sympathetic. They are emotional and afraid of being broken. They may be forced to make decisions, they tend to immediate response. They have natural ability for earning money.


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TOUR CODE: NAHTB 003 4 Days Tsavo East, Tsavo West and Amboseli National Park under Canvas Wildlife Trail Private Safari
Trip highlights: This is a four days, three nights camping safari. On this safari, you will be able to view various species of wild game including the lion, leopard, cheetah, the elephant and a host of other plains animals. You will also be able to see attractions like, Mt. Kilimanjaro, lava flows, caves and the famous and beautiful Mzima Springs. You will travel in a tour minibus and stay in tented camps.
Day 1: Nairobi- Tsavo East National park
Upon arrival at the Jomo Kenyatta International Airport you will met by our tour manager for a safari briefing then depart for Tsavo East National Park arriving in time for lunch and enjoy an afternoon game viewing drive till dusk.
The Tsavo East National Park has an interesting and diverse history including: the Waliangulu and Kamba tribes used the park as a hunting ground prior to gazette-ment; the first European to see Mount Kenya, rev. Dr. L. Krapf, journeyed on foot through this area in 1848; during the construction of the railway bridge over the Tsavo River in 1898, lions (the famed man-eaters of Tsavo) terrorized the workers, killing over 130 people before being killed by Col. J. H. Patterson. During World War 1, British forces along the Tsavo river to counter threats from invading German soldiers from Tanganyika (now Tanzania); the European explorer Captain Lugards a European explorer passed through the area on his way to Uganda.
Dinner and overnight at Tsavo East tented Camp or similar.
Day 2: Tsavo East - Tsavo West - Amboseli
Pre Breakfast game viewing drive, and then breakfast, departs for Tsavo West for game viewing till lunch time. Have parked lunch at Tsavo west National park and proceed to Amboseli arriving in the evening with game viewing enroute and check in at the Kimana tented Camp or Similar.
Amboseli National Park is renowned for its huge elephant herds. Africa's highest Mountain - Mt Kilimanjaro sits on the upper slopes of Amboseli National park. Mt. Kilimanjaro's snow white peak lies across the border in Tanzania. Seeing the Kilimanjaro hovering above the clouds in the early hours of the morning is quite an experience. For the enthusiastic photographer, animal pictures arising against the dramatic backdrop of the mountain is quite a thrill. Apart from the Elephants, Amboseli is a home to; buffalos, black rhinos, zebras, wildebeest and other plain animals.
Dinner and overnight at Kimana tented Camp or similar.
Day 3: Amboseli National park
Morning and afternoon game viewing drives.
The combination of scenery, people and animals is what makes the park fascinating. Beautifully situated at the foot of the towering Kilimanjaro, it is the home of the brightly painted Maasai tribe. You may be able to see lions, cheetahs, spotted hyena and wild dogs. Also the Kaffir Buffaloes, Burch ells zebras and the herds of elephants. All this animals come to drink water at the water holes consisting of small lakes or muddy bonds kept filled up by the melting snows of Kilimanjaro.
All meals and overnight stay at Kimana tented Camp or similar.
Day 4: Amboseli - Nairobi
Pre breakfast game viewing if you still want some more, After breakfast proceed to Nairobi, game viewing en-route and drive south to Namanga on the Kenya/Tanzania border arriving in time lunch at the famous Carnivore Restaurant. We will then transfer you to Jomo Kenyatta airport for your next destination.
End of safari.
Package includs:
-Transport based on a safari tour minibus with pop up roof for game viewing
-park entry fee
-accommodation and all meals while on safari
-service of an English-speaking driver guide, chef and food plus equipment
-Game drives as per the itinerary
-Driver allowances and park fees for vehicle and driver
-Government taxes
Package excludes:
-Tips to driver guides and porter
-Extras at lodges- tips, drinks, laundry
-Items of personal nature.
- Sleeping bags


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A bright red Alfa Romeo Spider was driven by Ben, Dustin Hoffman's character, in the movie The Graduate to the strains of the folk pop duo Simon & Garfunkel. It made a star of the actor, Dustin Hoffman, who hadn't acted before, the singing duo, and that bright red Alfa Romeo.
The 1967 Duetto is a two-seat convertible produced by the Italian car manufacturer Alfa Romeo from 1966 to 1993. Widely regarded as a design classic, it remained in production for almost three decades with only minor aesthetic and mechanical changes. It has become one of the most accessible cult Italian automobiles.
1967 Red Alfa Romeo Duetto RVL
It had many wonderful features: a responsive all-alloy twin-cam engine, accurate steering, sensitive brakes, a finely balanced chassis, and great looks. No wonder, the Spider sold over 100,000 units during less than three decades of its production run despite the same price tag as the much faster Jaguar E-Type (which could do 150 mph).
The Spider had huge trunks by sports car standards, the spare wheel neatly tucked away under the trunk floor It had dual Weber two-barrel side-draft carburetors, and produced 109 hp (81 kW). Sparsely fitted inside but including five speed manual transmission and independent front suspension, the car cost just $41 less than a comparable Lotus Elan in the US ($3,950).
The Spider's contemporary design makes it one of Alfa Romeo's great postwar cars. It was even called a poor man's Ferrari.
1967 Alfa Romeo Spider Specs
Production 100,000 units
1967 Alfa Romeo Duetto
History of the Automobile:
Forerunners of the Modern Automobile
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1920's Cars
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1960's Cars:
1960's Economy Cars 1962 Ford Falcon 1966 Corvair Monza
1960's Muscle Cars Dodge Charger Daytona Dodge Charger R/T 1966 Pontiac GTO
1960's Pony Cars 1964 Ford Mustang 1967 Shelby GT500 1967 Camaro Convertible 1964 Plymouth Barracuda 1968 Mercury Cougar
1960's Foreign Cars 1963 Austin Mini Cooper 1966 AC Cobra 427 1967 Alfa Romeo Spider 1960 Aston Martin DB4
Classic 60's American Cars 1963 Buick Riviera Dodge Charger Daytona Dodge Charger R/T 1962 Ford Falcon 1962 Ford Galaxie 500XL 1964 Ford Mustang 1967 Shelby GT500 1963 Corvette Sting Ray 1966 Corvair Monza 1967 Camaro Convertible 1964 Oldsmobile Starfire 1964 Plymouth Barracuda 1968 Mercury Cougar 1966 Pontiac GTO 1969 Corvette Stingray
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Devon Flat Front Wool Serge Trousers
An updated full fit trouser between heritage and contemporary. Constructed using a super 120s wool serge to be the essential year-round trousers. Our signature stretch waistband and high rise provide paramount comfort for everyday movement. The Devon is the new essential for the classic man.
Rise - 10''
Knee - 19.75"
Leg Opening - 16.5''
Length - Unfinished 46''
Model is 6'2", wears a 40R suit, and is wearing a size 32 pant.
100% Virgin Wool.
Dry clean only.


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Tony De Rosa is a member of the Barbershop Harmony Society joining at the age of 7. He grew up singing barbershop harmony under his dad's ("Papa Joe") direction with choruses in Rochester, N.Y., and Polk County, Florida. His life was immersed in barbershop harmony as he performed in his dad's choruses, listened to his mom's quartet, sang with his sister Chris in "The De Rosa 4", and followed his favorite quartets, learning their songs and adding to his repertoire. He hit the International stage as a tenor, at age 12, with a memorable rendition of "Where the Angels Live," sung by the "Cypress Chord Club." Seven years later, after many appearances at International with District Championship quartets, he captured a gold medal (The youngest ever to win in the Barbershop Harmony Society at 19!) with the 1992 champs "Keepsake." He attained this level again in 2000, repeating on the baritone part, as "PLATINUM" won the quartet championship. At present, Tony is the lead singer in the 2007 Gold medalist quartet "Max Q" (www.maxquartet.com), a distinctly artistic and talented foursome. Beyond that, he is a part of "Main Street" (www.mainstreetqt.com), whose jump into competitive barbershop landed them in 5th place at the international convention in both 2011 and 2012, 4th place in 2013 and 3rd place in 2014, 2015 and 2016. "Main Street" is dedicated to bringing traditional barbershop arrangements and look to the stage. Tony has years of barbershop directing experience. In his first venture, he continued his father's legacy by directing the Tampa Heralds of Harmony to International competition nine years in a row (1994 – 2002). He currently directs two barbershop choruses between the Barbershop Harmony Society and Sweet Adelines International. Since 2007, he has directed both the Toast of Tampa Show Choruswww.toastoftampa.org ) from Tampa, FL. Under his leadership, this group has competed at the International level where they finished 3rd in 2015! Tony was also awarded Sweet Adelines International Director of the Year in 2013! If that isn't enough, in January of 2012, Tony 'came home' to direct the Heralds of Harmony (BHS / www.heraldsofharmony.com ) from Tampa, FL once again. With great appreciation, his Barbershop life is full!!!! Tony lives in Winter Garden, Florida, and works as a freelance vocalist, as Music Director for the "Voices of Liberty" at EPCOT, the Dapper Dans of Disneyworld at the Magic Kingdom and the amazing new You Tube sensation Voctave, as a coach to numerous barbershop and Sweet Adeline choruses and quartets, and as an active learning-tape, rehearsal CD producer (www.advocals.com).
Those are Tony's barbershop and musical "stats", but his true pride and joy are his wife, Jodi, and his family. Jodi and Tony met when they both sang with the "Voices of Liberty," (Jodi sings that top soprano part!) and they've shared some amazing and challenging musical moments. Most important to Tony, they also share four terrific children: Alec and husband Eric, Kayla, Joseph (now proudly standing center stage with the Heralds of Harmony and competed in a quartet with Tony and another father/son combination!), and the youngest, Sophia. Watching the kids grow and change is Tony's greatest pleasure---and worry! Oh yes, and the family is enjoying their newest addition, grandbaby girl, Ella. That's right, he's a Grandfather! This family shares the blessings of strong faith in the Lord, extended-family fun, and a busy, but full life...loaded with music!


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Dame Carol Black's Review of the health of Britain's working age population
Key challenges and recommendations for reform
Key challenges
1 The economic costs of sickness absence and worklessness associated with working age ill-health are over £100 billion a year – greater than the current annual budget for the NHS and equivalent to the entire GDP of Portugal.
2 The evidence base to support the business case for investment in the health and well-being of their employees is inadequately understood by employers.
3) Lack of appropriate information and advice is the most common barrier to employers investing in the health and well-being of their employees. This is particularly true for smaller organisations which tend not to have access to an occupational health scheme.
4 The importance of the physical and mental health of working age people in relation to personal, family and social attainment is insufficiently recognised in our society.
5 GPs often feel ill-equipped to offer advice to their patients on remaining in or returning to work. Their training has to date not prepared them for this and, therefore, the work-related advice they do give, can be naturally cautious.
6 The current sickness certification process focuses on what people cannot do, thereby institutionalising the belief that it is inappropriate to be at work unless 100% fit and that being at work normally impedes recovery.
7 There is insufficient access to support for patients in the early stages of sickness, including those with mental health conditions. GPs have inadequate options for referral and occupational health provision is disproportionately concentrated among a few large employers, leaving the vast majority of small businesses unsupported.
8 The scale of the numbers on incapacity benefits represents an historical failure of healthcare and employment support for the workless in Britain. Furthermore, the flow of recipients of other benefits onto incapacity benefits suggests a failure in other employment and skills programmes to identify developing health conditions at a sufficiently early stage.
Pathways to Work, while successful overall, has had limited effect for those whose main health condition is a mental illness. Furthermore, over 200,000 people with mental health conditions have flowed onto incapacity benefits each year over the last decade.
9 Detachment of occupational health from mainstream healthcare undermines holistic patient care. A weak and declining academic base combined with the absence of any formal accreditation procedures, a lack of good quality data and a focus solely on those in work, impedes the profession's capacity to analyse and address the full needs of the working age population.
10 Existing departmental structures prevent Government from fully playing its part in meeting the challenges set out in this Review.
Main recommendations
1 Government, healthcare professionals, employers, trades unions and all with an interest in the health of the working age population should adopt a new approach to health and work in Britain based on the foundations laid out in this Review.
2 Government should work with employers and representative bodies to develop a robust model for measuring and reporting on the benefits of employer investment in health and well-being. Employers should use this to report on health and well-being in the board room and company accounts. Safety and Health practitioners and, where present, trades union safety representatives, should play an expanded role in acting to promote the benefits of such investment.
3 Government should initiate a business-led health and well-being consultancy service, offering tailored advice and support and access to occupational health support at a market rate. This should be geared towards smaller organisations. It should aim to be self-sustaining in the medium-term, and be fully evaluated and tested against free-to-use services.
4 Government should launch a major drive to promote understanding of the positive relationship between health and work among employers, healthcare professionals and the general public. This should include encouraging young people to understand the benefits of a life in work and its impact on their families and communities.
5 Building on the commitment from the leaders of the healthcare profession in the recent consensus statement, GPs and other healthcare professionals should be supported to adapt the advice they provide, where appropriate doing all they can to help people enter, stay in or return to work.
6 The paper-based sick note should be replaced with an electronic fit note, switching the focus to what people can do and improving communication between employers, employees and GPs.
7 Government should pilot a new Fit for Work service based on case-managed, multidisciplinary support for patients in the early stages of sickness absence, with the aim of making access to work-related health support available to all – no longer the preserve of the few.
8 When appropriate models for the Fit for Work service are established, access to the service should be open to those on incapacity benefits and other out-of-work benefits. Government should fully integrate health support with employment and skills programmes, including mental health support where appropriate. Government should expand provision of Pathways to Work to cover all on incapacity benefits as soon as resources allow, and explore how to tailor better provision for those with mental health conditions.
9 An integrated approach to working-age health should be underpinned by: the inclusion of occupational health and vocational rehabilitation within mainstream healthcare; clear professional leadership; clear standards of practice and formal accreditation for all providers; a revitalised workforce; a sound academic base; systematic gathering and analysis of data; and a universal awareness and understanding of the latest evidence and most effective interventions.
10 The existing cross-Government structure should be strengthened to incorporate the relevant functions of those departments whose policies influence the health of Britain's working age population.
Body mass index
A measure of body fat based on height and
(BMI) weight which applies to adult men and women. BMI is calculated as:
(weight in kilograms) / [(height in metres) x (height in metres)].
BMI categories are:
• Underweight = under 18.5
• Normal weight = 18.5 to 24.9
• Overweight = 25 to 29.9
• Obese = 30 or over
Cognitive behavioural therapy (CBT)
A treatment for mental health conditions which combines cognitive therapy (which can modify or eliminate unwanted thoughts and beliefs) with behavioural therapy (which can help change behaviour in response to those thoughts).
A state of complete physical, mental and social well-being and not merely the absence of disease or infirmity.
Occupational health
The promotion and maintenance of the highest degree of physical, mental and social well-being of workers in all occupations by preventing departures from health, controlling risks and the adaptation of work to people, and people to their jobs.
Public health
The science and art of preventing disease, prolonging life and promoting health through the organised efforts and informed choices of society, public and private organisations, communities and individuals.
Sick note
The sickness certification form issued by healthcare professionals to people of working age, normally required to claim Statutory Sick Pay.
State Pension age
For women 60 years, and for men 65 years. Due to rise to 68 years for both men and women by 2046 (in the UK).
The subjective state of being healthy, happy, contented, comfortable and satisfied with one's quality of life. It includes physical, material, social, emotional ('happiness'), and development and activity dimensions
[Waddell & Burton, 2006].
© Crown Copyright 2008
Produced by the cross-government Health, Work and Well-being Programme
Full article available from: http://www.workingforhealth.gov.uk/documents/working-for-a-healthier-tomorrow-tagged.pdf


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The Universities of Applied Sciences network (UASnet) aim to strengthen the contribution of the Universities of Applied Sciences (UAS) sector to the research and innovation strategy of Europe. The UAS are uniquely positioned, because of their integrated approach of educating initial and lifelong learners, based on innovative research results produced by working closely together with the world of work. Linking UAS by the European Network UASnet, ensures the European labour market of clustered knowledge and state of the art RDI solutions, hence, increasing the competitiveness of the EU.
See also


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Rainy Day Style Guide
This Way >
Wellies Vs Rain Boots
Rain Boot Care Guide
Our padded jackets are one of the reasons we love cold weather, and this style is no exception. Wadded for exceptional warmth and with flattering elasticated side panels it's the perfect balance of form and function. If you're looking for a new layer, look no further.
This jacket is close fitting so if you prefer a looser fit we recommend sizing up.
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I'm new to this forum so if this question has already been answered, please point me in the right direction.
I'm nearing the end of an extended stay (approx. 30 days) at a Fairfield Inn and although the entire staff treats me well, one employee in particular has gone over and beyond on at least two occasions. The first time, she guaranteed me a room when I couldn't book one online. The second time, the employee helped my wife (who isn't here with me) surprise me with dinner for our anniversary.
My wife and I really appreciate all the effort this employee has made. We'd like to know the best way to praise her in a way that might go beyond her immediate supervisor or manager. Should we write a letter to the general manager of the hotel? To corporate? Is their anything else we can do? This young lady has helped make us Marriot customers for life - the least we can do is tell her higher ups about the exceptional job she's been doing.
Thanks for anyone's suggestions.
- Salaam


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"Losing It In The Bush" (LIITB) empowers small and isolated rural communities of less than 5,000 people to take action to improve their own health in partnership with their community.
LIITB is a locally developed program empowering rural people living in the Port Macquarie and Kempsey regions to promote healthy lifestyle changes and reduce risk of complications from chronic disease. A partnership between rural communities, Port Macquarie Community Health (PMCH), Port Macquarie Hastings Council, Kempsey Shire Counciland At My Pace Specialist Rehabilitation.
The program operates out of rural halls or other venues. To book a place, call 6588 2882.
Sessions are two hours per week, including an exercise component and a presentation on healthy lifestyle by many health professionals incluiding dieticians, women's health nurses, mental health nurses and occupational therapists.
The program supports other national health programs such as:
- Get Healthy Telephone Line
- Swap it Don't Stop It


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|75 Global Minutes - Iridium Satellite Phone Service - PREPAID SIM Card 30 days
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|$175.00
Explorer Satellite Communications (ESCI) provides maritime, mobile, portable and fixed / stationary satellite phone solutions via several satellite networks – Inmarsat, Iridium, Thuraya, Globalstar and Thuraya. We sell, rent and offer world –class support for satellite voice, satellite internet, VSAT and SMS services. Markets served include Disaster Recovery, Business Continuity, Oil and Gas, Fishing, Leisure, Shipping, Exploration, Hunting, and Yachting.
Click below to download our App to send texts from your mobile device toyour Sat Phone.


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Mike and Max discuss Mike's cat emergency, their St. Patrick's Day weekend adventures in Toronto and going clubbing with "The Nut."
Nelly Furtado ("I'm Like a Bird," "Promiscuous") tells Mike about low-fi aesthetics, finding a location for the "Pipe Dreams" video, meeting St. Vincent, balancing business and art, her MoMA PS1 workshop, her plan for "Loose" and enjoying challenges.
For dessert, Shane describes his low-key night at a diaper party, an argument about circumcision, getting harassed by "The Nut" and the apology that followed.
The Mike on Much podcast can be found on iTunes, Google Play, SoundCloud, Stitcher and YouTube. Follow the guys on Twitter for updates and go behind-the-scenes on Instagram.


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// ]]>
Archive for December 30, 2009
Robert Pattinson on PopSugar's Top 10 Stories of 2009 3 comments
2009 in Black & White and Colour Enhanced Pics of Robert Pattinson 9 comments
As promised here's a recap of 2009 in black & white and colour enhanced pics of Robert Pattinson. I looked through tons of folders on my computer to find 100 pics. The majority of these I did. Some of the black & whites were done by Corr, who did the majority of our black & whites when we first started. We miss you Corr and wish you all the best in 2010.
More pics after the jump!
Robert Pattinson Among Moviefone's Best Celebrity Quotes of 2009 1 comment
"Really? He looks like he smells really good [laughs]. I also do shower." — On rumors that he and Zac Efron don't shower
Full Interview here
"[Laughs] I had so many ridiculous answers just come into my head [more laughs]. I had a penis implant!" — On his nude scenes in 'Little Ashes'
Full Interview here
New Edward Wallpaper 3 comments
Another great wallpaper from CandyKizzeS24! The dimensions are 1400×1050, click on it for the full size.
International Release Dates For Eclipse 30 comments
Argentina 30 June 2010
Brazil 30 June 2010
Egypt 30 June 2010
Finland 2 July 2010
France 7 July 2010
Germany 15 July 2010
Kazakhstan 30 June 2010
Netherlands 1 July 2010
New Zealand 1 July 2010
Norway 2 July 2010
Sweden 30 June 2010
Turkey 2 July 2010
UK 9 July 2010
USA 30 June 2010
New Moon Trailer Park's Number One Exclusive Trailer of 2009 1 comment
Current mood: Happy New Year!
Category: Movies, TV, Celebrities
Can you believe 2009 is almost over, and so is this decade along with it? We hope all of our friends have a very happy New Year. And since everybody makes lists at this time, we decided to count down our top ten exclusive trailer premieres from 2009. You'll NEVER guess what topped this list. 😉 Thanks for sharing your love of movies with us, and may 2010 bring good fortune and happiness to you all (and good movies too). Tell us what you think – what movie are you looking forward to most in 2010?
Trailer Park's Top Ten Exclusive Movie Trailer Premieres of 2009:
1. The Twilight Saga: New Moon
2. A Nightmare on Elm Street
3. Michael Jackson's This Is It
4. Bruno (restricted)
5. G.I. Joe: The Rise of Cobra
6. The Final Destination
7. Prince of Persia: The Sands of Time
8. Legion (restricted)
9. Sorority Row
10. Surrogates
New Robert Pattinson Wallpaper From The Summer House 2 comments
Here's a great new wallpaper made by CandyKizzeS24 of Robert Pattinson in The Summer House. Click on it to make if bigger


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Tech Guide has landed in Las Vegas for CES
CES was first held in New York in June 1967 before moving to Las Vegas in 1978.
Some of the new products unveiled at the show include the VCR (1970), LaserDisc (1974), Atari's Pong game (1975), Camcorder (1981), Compact Disc (1981), Commodore 64 computer (1982), Nintendo Entertainment System (1985), DVD (1996), High Definition TV (1998), DVR (Digital Video Recorder) (1999), Xbox (2001), HD DVD (2004), Blu-ray Disc (2004), 3D TV (2009), Smart TV (2011).
Early tomorrow morning (Aust time) is CES Press Day when the show's heavyweights – LG, Samsung, Panasonic and Sony – reveal their new products.
All of the product announcements will be covered right here on Tech Guide along with reports from the show floor, hands-on product demos and interviews.
The show, which is held at the Las Vegas Convention Centre, is attended my more than 150,000 people including exhibitors, industry analysts, retailers and press.
Check back with Tech Guide everyday for all the latest CES news.
Related Stories
Enjoy an iShower with all of your favourite music
New Xbox 720 will also be a digital video recorder
The tech products we can expect in 2012
LG to introduce new Ultra Definition TVs as CES
Samsung to unveil "future of Smart TV" at CES


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The poor in some cities — big ones like New York and Los Angeles, and also quite a few smaller ones like Birmingham, Ala. — live nearly as long as their middle-class neighbors or have seen rising life expectancy in the 21st century. But in some other parts of the country, adults with the lowest incomes die on average ...Read More »
San Francisco New Paid Leave Law: Another Job Killer?
Wednesday April 06, 2016 – San Franciscans are patting themselves on the back for being the first in the nation to guarantee that workers receive six weeks of paid leave following the birth or adoption of a child. Such a benefit sounds compassionate—everyone wants parents to have time to recover from birth and bond with their new family member—but we ...Read More »
Are There Health Benefits From Eating Walnuts?
Daily walnut consumption could have beneficial effects when it comes to delaying age-related health issues, a new study has found. Initial findings from the Walnuts and Healthy Aging (WAHA) study presented at Experimental Biology 2016 (EB) indicate that eating the nuts daily could positively impact blood cholesterol levels without adverse effects on body weight among older adults. The dual site ...Read More »
Study: Regular Marijuana Smokers Suffer More Work, Social and Economic Problems at Midlife Than Others
Saturday March 26, 2016 – A decades-long study has discovered that regular marijuana smokers experience more work, social and economic issues at midlife in comparison to the ones who use pot just occasionally or not at all. The University of California, Davis researchers tracked roughly 1,000 young people for decades and found that the ones who smoked cannabis four or ...Read More »
Supreme Court Hears High-Stakes Texas Abortion Case
Wednesday March 02, 2016 – The U.S. Supreme Court on Wednesday heard arguments in a major abortion case focusing on whether a Texas law that imposes strict regulations on abortion doctors and clinic buildings interferes with the constitutional right of a woman to end her pregnancy. Eight justices were on the bench as arguments began in the case, not the ...Read More »
Punxsutawney Phil Sees No Shadow, Predicts Early Spring
Groundhog Day 2016 – Punxsutawney Phil, the world's most famous furry forecaster, failed to see his shadow Tuesday morning, meaning spring is right around the corner, or so the folklore says. The groundhog's prediction came about 7:25 a.m. in Punxsutawney, Pa., with fair skies and temperatures in the mid-20s. If this winter's days truly are numbered, it will be remembered for both its mildness and brevity. Since ...Read More »
Franklin Graham discusses 'biblical values'
Taking a page from Jerry Falwell's playbook, Franklin Graham, son of the famous evangelist, has embarked on a tour of all 50 state capitals in an effort to encourage evangelicals to be politically active; he arrives in Concord on Tuesday. In the runup to the 1980 presidential election, Falwell conducted rallies at state capitols throughout the nation in what emerged ...Read More »


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Search & Read
Three Random Satsangs
ShiningWorld Reader
Be Your Own Guru
Mani: Dear James and Sundari, it's a long email, please feel free to pass it on after reading through the apologies. Also, it has some intimate details, but I know you will mask them if you feel fit to publish the letter (and reply, if any).
I enrolled in your and Sri Ted's Tiruvannamalai classes last year. To make a long story short, my parents and I came by car to Tiru for the start of classes, but had a change of heart and turned back to Chennai. The main driving factor was the fear of Vedanta and its reputation among middle-class Indians as a "career-killer." Coward that I was, I didn't even have the courtesy to contact you and inform that I may not be able to make it back to the classes. I didn't make it back. My (long-delayed) apologies.
The Mid-Life Crisis
Please note the liberal use of the word "I" below. I had the experience of akhandakara vritti (the self) last year, but did not capitalize on the experience and use it to build on knowledge. I am now starting again. Context: I discovered Vedanta and Sri Ramji's lectures in the middle of my marriage disintegrating last year. Bhagavan in his great mercy gave me the right knocks to accelerate the search for self. I have heard Sri Ramji's lectures several times, especially on self-enquiry, though I am yet to finish the book. At this stage, the teachings (especially on karma yoga) are starting to appear by themselves at the right times to solve problems and bring peace. And again, I am thankful.
I have now wrapped up my old life, experienced some level of financial loss and am separated from my wife – on the way to a divorce. I am trying to rebuild my life. This body's sexual frustration led me to online chat rooms where anonymous men and women titillate each other with words and images. This was like adding kerosene to the fire, and I was fast on my way to falling into a deep, dark place.
In the midst of this, I started chatting with a 25-year-old woman, also on the site to get over a break-up. We felt comfortable enough with each other to share our real-world identities. Our first conversation over video chat was two hours long. It's been three days and this body-mind is in the throes of a "crush" that has taken away sleep, a sweet pain permeates and this mind has thoughts of her all the time.
It's funny, I am 38, not a spring chicken, and even as of a week ago would have thought I was long past this. She is worldly-wise, experienced beyond her years, and has made it clear she is not interested in anything beyond a friendship. I know this is right and wise. This situation has already given me enough fruits, including pulling me completely out of the bad situation just three days ago. I am taking the karma yoga approach, thanking the Mother Goddess for her prasad. This woman is now a muse, fuelling my creativity and setting me back on a more sattvic path.
1. Beyond this, do I just disregard the "sweet pain" and wait for it to go away as another emotion, just hormones working on a guy going through a mid-life crisis?
James: Yes. That's the mature attitude.
Mani: 2. While I get the many benefits from having this woman in my life, for as long as Bhagavan deems fit, what do I owe her (the field of existence)? I am afraid my whiny/immature presence around her would detract from her pursuits at this early phase of her life.
James: You owe her a polite, sincere, civilized communication, that's all. Speak to her with honesty, like you speak to me. Ask her to excuse the whiny, immature part of yourself. It isn't you; it's the result of conditioning. She will understand. If she doesn't, you don't need her.
Mani: The power of maya is truly awesome, the right approach would of course be to dismiss this all a mithya, but I don't see this body-mind at that level of maturity today.
I owe you another apology for wasting your time and flooding your inbox. Please ignore anything except the apology.
In the light of day, I can see the rest is unmitigated drivel unrelated to Vedanta. I can now see that I need to:
1. Give thanks to the field for pulling me away from the bad situation I was in a few days ago.
James: Yes, indeed.
Mani: 2. Start reading your book again from Chapter I. I will do the work before writing to you my next email. My thanks again.
James: Vedanta is a program. It is totally logical and practical. Once you see the big picture, you have something to focus your mind and a way to evaluate your progress.
Mani: Sheepishly yours, Mani.
James: Hi, Mani, I was just about to write to you and tell you to man up and quit whining when you figured it out on your own. Good for you. Vedanta need not be a career-killer. That is what the Gita is saying. Arjuna wants to head for Rishikesh so he doesn't have to do his job, but Krishna says that he should do his job to work out his vasanas but do it as karma yoga which prepares him for moksa. In your case, you may have learned that security and pleasure are pipe dreams and if you are dispassionate enough, you may be qualified for the second stage of karma yoga, naishkarma karma, action without desire for objects. This is a more advanced stage because you have to resist your desires. When your desires for security and pleasure no longer bother you, you are ready for sanyassa yoga, or Vedanta, i.e. self-inquiry.
~ Om and prem, Ramji


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Dream South Beach
Read more about this Hotel
This stylish hotel offers a certain charm and elegance which is evident the minute you walk in to the lobby. Very popular hotel in a great location steps from the beach and right in the heart of all the action. Facilities include swimming pool, restaurant and roof top bar.
How far is the nearest beach?
This hotel is located 50m from the beach.
Distance from Airport
This hotel is located 18km from Miami Airport.
Rooms offer air conditioning, flat-screen tv, free WiFi, and iPod docking station mood lighting, WiFi, in room safe, hairdryer, bath robes.
Food & Drink
Restaurant and a roof top bar.
Sports & Entertainment
Swimming pool and WiFi.
- Air Conditioning
- Bar
- By the Beach
- Hairdryer
- Internet Access
- Reception
- Restaurant
- Safety Deposit Box
- Sunbeds
- Swimming Pool
- Wifi


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This is an image of Rixy, on the wire ladder. That ladder was bloody tricky to work with, but Rixy nailed it. Shot during the day, with Rixy on one boat, me on another (trying not to fall in). I think I have some bts shots from Natalie Branco. Natalie assisted me for this shoot, and in fact did a post on her blog of the experience. I love the mystery, the location, and the boys looked great. Enjoy.
, by Unknown


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Mobile Legends Heroes Review: Karrie The Lost Star
Hello and good morning guys, welcome back to my website. This day I want to share with you about mobile legends heroes review. Well, my review this day is about Karrie the Lost Star. So, how much you know about… Continue Reading


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"Hey! Hey! -Tell your pal that he's just cost me my job."
One of the last haunted cries of Graham Taylor's reign as England gaffer, chasing a helpless linesman down the Rotterdam touchline after the referee failed to send off Ronald Koeman for upending David Platt as he ran through on goal. Yes, the Ronald Koeman who, naturally, went on to score the free kick that ensured that England wouldn't be going anywhere near the 1994 World Cup Finals.
In reality, poor old Taylor would soon lose much more than his job. Already christened Turniphead by the Fleet Street wags, the Channel 4 documentary "An Impossible Job" – which focused on Taylor's management through that qualifying campaign – would strip him of the remainder of his dignity and coin the classic managerial phrase of the time. "Do I not like that!"
In the program, Taylor's increasing desperation – and sidekick Phil Neal's unrelenting sycophancy – was vividly exposed during another abject England showing in Poland, where that great philosophical puzzle "Can we not knock it?" got its first airing:
Des Walker knocks a misplaced pass to John Barnes:
Taylor: "Ooooh, fucking… Do I not like that!"
Poland win the ball, break downfield and score.
Taylor: "What a fucking ball. What a ball, eh, from Des to Barnesy. What a fucking… It was our possession."
Phil Neal: "I know."
Taylor: "It was from our free kick. We've come square, and the ball… Des and Barnesy, eh? Fucking ball, eh? You can talk till you're fucking blue in the face, can't you?"
Phil Neal: "Yes boss."
The game kicks off again:
Taylor : "Come on. Bigger, bigger."
Another misplaced pass.
Taylor : "We've done that fucking… CAN WE NOT KNOCK IT? They've done everything that we told them not to do. Everything that we told them not to do."
And the full misery of the Dutch denouement was compelling, if heartbreaking, viewing:
Taylor: "Linesman, linesman, what sort of thing is happening here? You know it, you know it, don't you? Absolutely disgraceful."
Linesman mutters something.
Taylor: "Linesman, linesman, that's disgrace… Hells Bells!"
Koeman hasn't even scored the free kick yet. In the gantry, Brian Moore is first to cotton on to what's about to happen.
"He's going to flip one. He's going to flip one. HE'S GOING TO FLIP ONE."
Koeman has scored.
Taylor: "I'll tell you, they don't fucking deserve." "Fucking. That is absolutely shocking."
Businesslike for a moment.
Taylor: "We'll have to get Wrighty on shortly."
Phil Neal: "We'll have to give Wrighty a go."
Taylor turns his attention once more to the beleaguered linesman.
"You know we've been cheated, don't you."
Linesman motions him back to his technical area.
"I have a metre. I have a metre. You know. It's alright."
Linesman is getting fed up. Goes to report Taylor, who pleads for mercy.
"I won't say anything else. Come on, don't. But I'm allowed to stay in the metre."
Linesman lets him off with a warning. However, the peace bond is soon broken:
"Even if he doesn't see it as a penalty, he has to go. You know that. I know you know it, so… And then the fella scores the free kick."
A sudden outbreak of bonhomie from Taylor:
"You can't say anything. I know you can't say anything. I know that."
Again it's short-lived:
"But, you see at the end of the day, I get the sack. Will you say to the fella, the referee has got me the sack."
"Thank him ever so much for that, won't you?"
Although he worked again in the game – and with a degree of success too – Taylor's reputation has never quite recovered. Like David Pleat nowadays, the work of Taylor the co-commentator has rather surpassed that of Taylor the gaffer. After all, no-one has yet superimposed a root vegetable on his shoulders after a slipshod turn in the gantry. It may, however, be simply a matter of time.
"In football, time and space are the same thing."
Graham's special turnip continuum puts Einstein and co in their places.
"Well, it's a love-hate relationship and he loves me."
Graham Taylor speaks glowingly of his working relationship with Villa chairman, Doug Ellis.
"Shearer could be at 100 percent fitness, but not peak fitness."
One of those managers who always looked for 110 percent.
"Sometimes it's very hard to follow what would have happened and sometimes it's hard to follow what has happened."
…and sometimes its very hard to follow Graham Taylor.
"To be really happy, we must throw our hearts over the bar and hope that our bodies will follow."
As Phil Neal might say, "That's right, boss."
"Very few of us have any idea whatsoever of what life is like living in a goldfish bowl – except, of course, for those of us who are goldfish."
Once a turnip, now reincarnated as a goldfish. Suppose they're roughly the same colour.
"I'd never allow myself to let myself call myself a coward."
Nor would we prevent ourselves from stopping ourselves calling you a guff merchant.
"What a clinical finish. That's got nothing to do with his haircut at all. That's footballing ability."
Hard-but-fair Graham scuppers any chance Clint Mathis' barber had of claiming an assist for the USA's goal against South Korea.
"I sometimes wished I'd been shot…though it never came to that… nor should it."
Musing on his time as England manager, Graham lectures a chastened public on the rights and wrongs of football manager assassination.
"The thing about coming to watch football in this part of the world is it makes you realise what a world game it is."
Two-thirds of the way through the World Cup, Graham eventually cottons on to what's been happening for the last three weeks.
"There may have been a problem with the wall of two or two and a half players."
Graham reveals that at least one English player lost his head before a Portugal goal."
"Nothing that UEFA or FIFA do surprises me any more and I'm very surprised this has not been sorted out long in advance."
Nothing more surprising than the element of surprise.
"He knows they've got to score three goals. They know they've got to score three goals. What more can you say?"
Graham showcases the tactical awareness he brought to the England job.
"That's a goal, isn't it, but what a good save!"
It wasn't and it was.
"Sometimes it's very hard to follow what would have happened and sometimes it's hard to follow what has happened."
And sometimes it's very hard to follow Graham Taylor.
"I think what would help the Ecuadorian side is if they could get a glimpse of the possibility of scoring a goal."
Do you reckon, Graham?
"Their hospitality has been fantastic and a good example to many other countries. I can't think of one…"
Probably better that way, Graham.


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Want to lose weight? Or maintain the weight you've got? For many of us, weight management is a great idea.
Research also shows that extra weight increases your risk of cardiovascular disease, diabetes, cancers, gall bladder disease, osteoarthritis, sleep apnoea, infertility, respiratory problems and even gum disease. According to research, putting on as little as four kilograms may increase the risk of heart disease in a healthy adult.
What you can do about it
"Daniela, view this message as a whole-hearted recommendation that weight-watchers doubting your worth may call me. From my referral to you, I doubted you could fix my knee - you did. I also doubted you could program me to lose weight - you did. But – until today –, I never thought, ever thought you could give me the tools of confidence to carry on by myself! Cheers and thanks... you're a star!"
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"I had been struggling with my weight for many years. Tried all the various programs, was active with gym and a personal trainer to build up my strength and increase my fitness level. But I still struggled with weight. I approached my trainer one day who is an ex First Grade Footballer and he recommended Kate. From my first meeting with Kate I was very impressed. The meetings were very informative, Kate gave me lots of strategies and answered any question I had concerning not only food but lifestyle too. I have been seeing Kate for two months and I have lost five kilos. Kate is a very enthusiastic person with a wealth of knowledge, who loves what she does. I enjoy meeting with Kate, because each session gives me more and more information at how to achieve my goal."


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