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Colors are important. There are many things in life that colors can reveal or reflect. Like fruit, for instance. How can you tell if a banana is ripe, not ready or rotten? That's easy, it's all dependent on the color. Yellow means perfect, while black means rotten and green means it's not ready to consume yet! It's fascinating yet simple.
Only Smart Enough People Can Pass This Hit-The-Dot Test. Can You?
This also applies to humans, but not in how ripe they are, lol. As far as people are concerned, colors can represent many aspects, especially gender. You might be surprised to know that gender governs what color we might be drawn to.
Here are a few interesting facts: Men love greens, grays, and oranges while women love pinks, purples, and reds. Both men and women love blue, men love darker deeper hues while women prefer the lighter tones.
This is familiar to us and businesses market products according to these color distinctions. Men's packaging is often dark whereas women's product packaging is generally white or in light colors. These links are strong and research has concluded that there is a difference in what women like as opposed to men.
TEST: This Math Equation Is Breaking The Internet. Can You Figure Out The Right Solution?
The following color test can tell you which gender is more dominant. Despite what you were born with, colors can determine what you really are, whether you are male, female or unisex. Enjoy!
Credits: Inspire2Day

subset: commoncrawl


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and more of same
and more of same
brown and green scarf
The Sherington Coat of Arms Sir Henry Sherington 1430-1497 He was my 14th Great Grandfather
This is one of my favorite things about knitting. I am currently knitting a "shawl" – what color do I choose next?
puzzle finished – was enjoyable, actually compulsive – time-wasting?
Katerina must be satisfied with her breakfast – she is occupying chair #2
Yesterday – Katerina ousted me from chair #1 – MY CHAIR
Shades of green and yellow – Knitted Hat 12


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GCN to Runoff 2017
A java program that reads GCN precipitation files ( CSV) as input and computes SCS curve numbers 30 - 99.
This program expects precipitation files downloaded from the GCN Daily Climate Data website: https://gis.ncdc.noaa.gov/maps/ncei/cdo/daily. An example is included in the zip file. Match the format of the example. User needs to monitor quality of observation data.
When you download from GCN make careful note of the units you select, it is not obvious later. Use one file for each station, formatted as daily rows. Do not use monthly or summarized data.
Each one of all CSV files found in the folder with this program will be processed as precipitation for one station. Daily runoff is computed for every observation. Then all runoff for each year is summed. User must have alread evaluated if sufficient observations exist for that year within the downloaded CSV file. After each year is summed, all years are averaged into a single Average Annual Runoff. Results are average annual runoff produced in inch or millimeter for all CN between 30 and 99. It is your responsibility to ensure the number of observations fully represents the year. Meaning you may need to remove years that have missing observations above a threshold you feel is appropriate.


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Immortal Venus Color Pro Lenses – Pacific Blue
- Description
- Additional information
- Discussion (2)
Amazing Immortal Venus Pro Color Contact Lenses
USA Contact Lens Verification
We use VerifyMyLenses to manage the contact lens verification to legally sell you lenses under the Federal Contact Lens Rule. If you are a USA customer you will receive an email from VerifyMyLenses requesting you to login and provide your Eye Care Professionals information after you checkout from our store. This email gets generated within 5 minutes of you submitting your order. Please make sure to check your spam folder if you do not get it. You must complete the form completely. Remember! Even lenses that do not correct your vision require approval from your eye care professional. This is a Federal Law and is done to protect the health and safety of your eyes.
Providing inaccurate or intentional incorrect information will not result in a verification. It will just seriously delay / cancel your order. If you are in the USA the law applies to you.
International Customers: Although we are not required by law to verify with your Eye Care Professional we highly recommend discussing the purchase with them to make sure they will be appropriate fit for your eyes.
We are serious about protecting the vision health and safety of our customers.
Additional information
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|4.0000 × 6.0000 × 5.0000 in
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In today's marketplace, finding appropriate credit union-specific training to achieve your business lending goals is difficult.
The new CUNA Business Lending Roundtable, Dec. 8-9, 2015, in Tempe, Ariz., is an advanced program that combines expert Q&A panels with networking and brainstorming events to provide an in-depth look into hot business lending topics.
You'll discover need-to-know updates from Vincent Vieten, MBL program officer at NCUA, and Linda Rusche, director of the office of financial assistance at the U.S. Small Business Administration.
Additional topics include:
Visit training.cuna.org/bizlending to register.


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Skin Care – Healthy or Safe for Us?
SkinCare products are not as healthy as you assume. Most people believe the regulatory powers would not allow toxins in cosmetics and skin care products.
You think the FDA plays a protective role between you and the product manufacturers. That assumption is not correct. Melissa Sherwood wrote a fascinating article on this subject, FDA Guidelines vs. Organic Natural Skin Care.
Even more shocking is finding out what it in our cosmetics and skin care products and the health risks they pose to all of us. Both men and women use skincare products, so the risks are the same.
For a list of the shocking Toxic 12 ingredients in most skin care, read this important article by Melissa Sherwood, The Ugly Truth About Beauty & Skin Care Products.
As you can see by the list on the right, finding healthy skin care products is important. The importance magnifies the younger the user.
Why is Toxic Skin Care Important to You?
The process of developing and putting a skincare product on the market can be shrouded in mystery, and sometimes regulations fall short. Believe it or not, the FDA doesn't need to approve what ingredients are added to personal care products or cosmetics and can only request, not order, that a company pull a skin care product, regardless of what's in it.
The skin acts as a passageway from the outside world to the bloodstream, so what we put on our skin can be absorbed by the body and enter our blood. Our skin can absorb up to 60% of what we slather onto it and the sensitive areas around our faces and armpits can absorb two to six times as much as
There is toxicity everywhere. As a result, the "it's just a little bit" from one product is a smoke screen. Looking at the cumulative of all those exposures for all the skin and personal products over a lifetime can add up to a chronic disease. The same is even more important for our kids, and grandkids as their mental and physical development go on for years.
Our Answer to SkinCare Toxicity
Natural or Organic claims are better than no such claims. But the real test is in the ingredients labels. However, reading deceptive labels can be a challenge. So we will help you get past the hype and recommend what we believe to be safe or "safer" products in our reviews.
If you want us to review a product, please contact us [contact page link] and maybe add it to our growing list.


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NOW! Your Donation Will Be Matched!
- A donor has come forward and offered to cover half of the costs of our expansion, NIS 25,000 ($7,000,) challenging us to match his grant, shekel for shekel, dollar for dollar, to raise the other half.
- Your donations will be matched by this donor. You can be part of this incredible initiative, helping indigent Israeli breast cancer patients in financial crisis due to illness!
- Please consider a donation of an average grant, NIS 2,100 ($590,) or for ANY AMOUNT towards our goal of NIS 25,000 ($7,000,)
Typical story. Last week the Lemonade Fund received an urgent request from a city Social Worker to pay the utility bills of a breast cancer patient who is also a victim of domestic violence. Single mother with 4 children. We did…
The Lemonade Fund is the only Breast Cancer Emergency Relief charity in Israel, and over the last year we have doubled the amount of money we are giving due to the pressing need.
Thank you!
To donate: https://lemonadefund.org/to-donate/
Lemonade Fund Requesting Emergency Aid for the New Year
- N., 34 years old, diagnosed with advanced breast cancer while pregnant with her third child. N. gave birth, but has been and will be hospitalized for while. Husband needs to hire a mitapelet (babysitter) to help him care for the infant and other two children so that he can work. Extended family and husband have used up all of their work vacation days and extra funds. N.'s Social Worker is appealing to the Lemonade Fund to help this family.
- K., 42, is a divorced mother of three children, a victim of domestic violence. Despite this, she had worked hard in the last few years since leaving her husband, to start a new life. She took out a loan to go back to school, but was then diagnosed with breast cancer and couldn't work or stay in school through the treatments. She is the sole support for herself and her children and is now in severe financial distress. The Lemonade Fund will help her get through this difficult period.
- A., 32 years old, lives in a large village in the Galil, far from the hospital where she is being treated for stage 4 breast cancer. In addition, her young son was recently diagnosed with leukemia and he is being treated in a separate hospital. Though her husband and family are very supportive, the travel costs for her treatments are significant. The formerly solvent family is being pushed to the limit by the auxilliary costs of these serious illnesses. A.'s Social Worker submitted an application to the Lemonade Fund for help with transporation costs, NIS 2500.
Three examples of current need at the Lemonade Fund. Many others as well. All donations are tax deductible, in Israel or the US. Thank you.
Young Family Asking for Help
The Lemonade Fund was notified of a family in the Sharon area that needs help. The mother, suffering from late stage breast cancer, is in intensive care. The father said that what he and his children want most right now is for people to pray (and recite psalms) for his wife. He said it would comfort them greatly to know that this is happening. His wife's name is Bracha Rachel Yetta bat Hindel. (The name Bracha was added this week.) At some later point we may be called on to help the family in other ways, such as with meals or financial assistance, but for now they are asking only for prayers. Thank you.
Breast Cancer Patient requests your prayers and good deeds
A good friend of the Lemonade Fund, Etana Sarah bat Hadassah, is herself undergoing a biopsy today to determine the extent of the spread of cancer to her abdomen and liver. She has asked that we pray for her and do good deeds. She is an amazing woman who has never complained, has only asked, during each bout with this illness, that we pray and do good to others, in her name. She may see this as part of her 'medicine,' and it has been working. Many have been helped, thanks to her. She has been fighting this disease for a while and we are with her in the hope that our actions can help her continue in her fight.
Thank you.
The Lemonade Fund
Social Workers at Meir Hospital have contacted the Lemonade Fund for help with food prep for a very ill single Mom from Kfar Saba. To sign up to prepare and deliver a meal please write to:email@example.com
Lemonade Fund offering meal assistance to local breast cancer patients
The Lemonade Fund is an Israeli organization that gives financial assistance to needy Israeli breast cancer patients who are in active treatment, Recently, a local friend and supporter of the Lemonade Fund needed help herself… help of a different kind. She knew it would be challenging to prepare healthy meals, for herself and her family, while undergoing chemotherapy for breast cancer. The Lemonade Fund asked for volunteers to cook meals for her, and the response was overwhelming. At the same time, a social worker at a breast center in a nearby hospital mentioned that there are some patients who are completely alone, and who could use a kindly delivered, home-cooked meal more than anything, We are seeing a need, one that our generous community is able and eager to fill, and thus was born the idea of adding meal assistance to the menu of services offered by the Lemonade Fund.
If you'd like to prepare a meal now and then for a patient from the Raanana/Herziliya/Kfar Saba area, please send an email to email@example.com and the Lemonade Fund will let you know how to sign up. The process is simple and straightforward and doesn't involve long term commitment. Can even be a one-time thing. Please share this post.
How lucky are we that we can come together to help each other in this way! Thank you.
Teenagers looking for summer volunteer opportunity?
This is a position that can be done in the comfort of your own home, alone or with friends, using a screen, even Facebook! The Lemonade Fund has pages of content, on our website and Facebook page, written in simple English, that need to be translated into Hebrew. (On another note, eventually we would love to translate into Arabic, Russian and Amharic, as well. Feel free to dive into these languages, too!) We also have plenty of other opportunities involving the continued development of our website and our Facebook page. No professional experience required, just enthusiasm and energy. Please contact the Lemonade Fund at firstname.lastname@example.com


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I sorta didn't want to post this...fine I really didn't want to because I really want to win these gorgeous earrings!
Only then I realized...if I don't win then I at least want one of you lovelies to win. So here you go...Friday fun! Go to this blog to win...
I am waiting for my FI to get home from work so we can have a little dinner date...until then I will be swooning over these earrings and dreaming of this dress I want from Chickdowntown to pair them with for a romantic little rendevouz on our honeymoon in three months.
Friday, July 10, 2009
Fab Giveaway Time
3 comments on "Fab Giveaway Time"
- . on July 10, 2009 at 10:52 PM said...
Those earrings are lovely!!
- . on July 11, 2009 at 11:43 AM said...
Hey girl. It says full price on the items, but once you get to your shopping cart, it will automatically give you the discount. You have until the 15th, so hurry! =)
- Allie on July 11, 2009 at 2:23 PM said...
Thanks for entering my giveaway! Good luck :)
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FOLLOW OHIO VOLLEYBALL: Facebook | Twitter
ATHENS, Ohio -- The Ohio volleyball team (5-8, 0-0 MAC) begins Mid-American Conference play this weekend. The Bobcats open up the league schedule on Thursday (Sept. 20) with a showdown with East Division rival Kent State (7-7, 0-0 MAC) on Thursday (Sept. 20) at the Convocation Center, then hit the road to take on West Division foe Central Michigan (3-9, 0-0 MAC) on Saturday (Sept. 20) at McGuirk Arena in Mount Pleasant, Mich.. Both matches are scheduled to get underway at 7 p.m. EST.
Thursday's matchup between the Bobcats and the Golden Flashes will be streamed live on ESPN3, with Jake Hromada, 'The Voice of Ohio Volleyball', and Gabe Genovesi on the call.
Thursday, Sept. 20, 2018
7 p.m. EST | Ohio vs. Kent State | Convocation Center | Athens, Ohio | Live Stats | ESPN3
Saturday, Sept. 22, 2018
7 p.m. EST | Ohio at Central Michigan | McGuirk Arena | Mount Pleasant, Mich. | Live Stats | Live Video
Ohio concluded the non-conference portion of its 2018 schedule last weekend at the Mizzou Invitational in Columbia, Mo. The Bobcats fell to Drake, 3-2 (14-25, 25-23, 25-20, 18-25, 15-8), and host Missouri, 3-1 (25-23, 21-25, 25-18, 25-12), on Friday (Sept. 14) but closed the weekend out with a 3-0 (25-19, 25-22, 25-21) sweep of Texas A&M-Corpus Christi.
Redshirt senior outside hitter Jaime Kosiorek (Chagrin Falls, Ohio) led Ohio with 14 service aces (1.17 per set) while ranking third in both kills (28; 2.33 per set) and digs (22; 1.83 per set) over the three matches. She earned a spot on the All-Mizzou Invitational Team for her efforts. Kosiorek recorded a match-high five service aces to go along with nine kills and 11 digs in the loss to Drake. In the weekend finale, she led the match with six service aces and ranked second on the team with nine kills in the sweep of Texas A&M-Corpus Christi.
Redshirt junior middle blocker Simone Miller (Kalamazoo, Mich.) led Ohio with 18 total blocks (two solo, 18 assisted; 1.50 per set) last weekend. She set a career high with 10 total blocks (one solo, nine assisted) in the loss to Drake.
Ohio went 2-1 at the 2018 Bobcat Invitational presented by Texas Roadhouse at the Convocation Center two weekends ago to clinch a share of its home tournament title. The Bobcats opened the weekend with a 3-2 (25-27, 24-26, 25-22, 27-25, 15-11) triumph over Samford on Friday (Sept. 7) morning. After falling to Virginia Tech, 3-1 (24-26, 25-21, 25-21, 25-16) on Friday evening, Ohio closed the Bobcat Invitational out with a 3-1 (25-15, 17-25, 25-17, 25-21) win over IUPUI.
Redshirt senior right side hitter Katie Nelson (Regina, Saskatchewan) enjoyed a big offensive week last week for the Bobcats, hitting .312 and ranking second on the team in both kills (46; 2.71 per set) and blocks (17; two solo, 15 assisted; 1.00 per set). Nelson started off week three of the 2018 season by tallying five assisted blocks in Ohio's 3-1 (21-25, 25-21, 20-25, 22-25) setback at Marshall last Wednesday (Sept. 5) at the Cam Henderson Center in Huntington, W. Va. She then posted double-digit kills in all three matches of the Bobcat Invitational. Nelson racked up a team-high 16 kills and added seven total blocks (one solo, six assisted) in the triumph over Samford. Later that evening, she hit .367 with 14 kills and chipped in two total blocks (one solo, one assisted) in the loss to Virginia Tech. She finished the weekend tournament strong, hitting .391 with 11 kills and totaling three assisted blocks as Ohio knocked off IUPUI.
Miller led the team and ranked fourth on the squad with 25 kills (1.47 per set) and 37 points (2.21 per set) last week. Miller opened week three of the 2018 season by matching her career high with seven assisted blocks in the loss at Marshall. She then put together a career performance in Ohio's triumph over Samford, hitting .650 with a career-high 14 kills and tallying a career-high eight assisted blocks.
Sophomore middle blocker Tia Jimerson (Sugar Hill, Ga.) was named the Mid-American Conference East Division Offensive Player of the Week, as announced by the league office on Sept. 4.
Jimerson became the first Bobcat to earn conference weekly award honors in 2018 and earned MAC East Division Offensive Player of the Week recognition for the third time in her career.
Jimerson helped the Bobcats go 1-2 at the Ameritas Players Challenge in Lincoln, Neb. Ohio fell to then-No. 5-ranked Nebraska, 3-0 (25-17, 25-14, 25-17), then suffered a 3-0 (29-27, 25-18, 25-20) setback to Santa Clara on Aug. 31. The Bobcats closed out the weekend on a high note, sweeping Wake Forest, 3-0 (25-20, 25-17, 27-25), on Sept. 1.
Jimerson hit a team-best .409 with a team-high 24 kills (2.67 per game). She also ranked second on the squad with 10 total blocks (10 assisted; 1.11 per set), helping her lead the team with 30 points (3.33 per set). Jimerson hit .429 with eight kills and also chipped in three assisted blocks last Friday as the Bobcats fell to Santa Clara. On Saturday, Jimerson tied for the match lead with 11 kills and hit .529 as Ohio swept Wake Forest. She also led the match with four assisted blocks.
Ohio began the 2018 campaign by going 1-2 at the Sports Imports Classic two weeks ago in Columbus. Sophomore setter Vera Giacomazzi (San Vendemiano, Italy) dished out a match-high 49 assists. She represented Ohio on the All-Sports Imports Classic Team after finishing the weekend with 94 assists (8.55 per set).
The Bobcats are coming off of a 2017 season that saw Ohio go 17-15 -- including a 10-6 mark in MAC play. Ohio earned the No. 4 seed in the 2017 MAC Championship and a bye to the tournament quarterfinals. Ohio topped Eastern Michigan, 3-1, to advance to the league tournament semifinals for the 16th year in a row.
Ohio was selected to win the MAC East Division, regular season and tournament titles in the league's 2018 preseason coaches' poll, the conference office announced on Aug. 22.
The Bobcats earned nine of the MAC East Division's 12 first-place votes and finished with 68 points. Ohio earned eight of 11 votes to win the regular season crown and five of 11 votes to win the tournament title. The poll was voted on the conference's 12 head coaches, with one head coach choosing not to select a regular season or tournament champion.
As a team, Ohio ranks second among MAC teams and 40th in the country with 75 service aces and third in the conference and 81st in the nation with 1.53 service aces per set. The Bobcats also rank fifth in the MAC and 61st in the country with 106.5 total blocks and fourth in the conference with 2.17 blocks per set.
Giacomazzi, Jimerson and Stephens represented Ohio on the 2018 Preseason East Division All-MAC Team.
Stephens, who was named a team co-captain on Tuesday, played in all 32 matches and appeared in all 122 sets in her 2017 sophomore season, earning All-MAC First Team honors. She led both the team and the MAC and ranked 66th in the country with a career-high 443 kills (3.63 per set), which ranks as the eighth-most in a single season in program history in the rally scoring era. She posted double-digit kills 25 times -- including 20 or more three times. Stephens led both the team and the MAC and ranked fifth in the nation with a career-high 60 service aces, which ranks as the fourth-most overall in program history and most in program history in the rally scoring era. She posted three or more service aces on 10 occasions. Her 0.49 service aces per set led both the team and the MAC and ranked sixth in the country and rank as the most in program history in the rally scoring era. Stephens led the team and ranked second in the MAC with a career-high 527.5 points (4.32 per set), which ranks as the ninth-most in program history in the rally scoring era. She posted 20 or more points 10 times. Stephens also ranked second on the team with a career-high 320 digs (2.62 per set) and posted double-digit digs 15 times -- including 20 or more two times.
Through 13 matches in 2018, Stephens ranks second on the team in kills (113; 2.31 per set), service aces (18; 0.37 per set) and digs (108; 2.20 per set).
Giacomazzi played in 32 matches and appeared in 121 sets in her 2017 freshman campaign, earning MAC Setter of the Year and All-MAC First Team honors as well as a spot on the MAC All-Freshman Team. She ranked second in the MAC and 30th in the country with a team-high 1,278 assists (10.56 per set; fourth in the MAC) -- the most assists in a single season by an Ohio freshman in the rally scoring era in program history, the ninth-most assists in a single season by any player in the rally scoring era and the 10th-most in a single season by any player in the overall history of the program. She tallied 40 or more assists 17 times, 50 or more assists eight times and 60 or more assists once. Giacomazzi ranked third on the team with 306 digs (2.53 per set) and posted double-digit digs 14 times. She achieved 14 double-doubles. Giacomazzi also ranked third on the team with 52 total blocks (two solo, 50 assisted; 0.43 per set).
Through 13 matches in 2018, Giacomazzi leads Ohio with 322 assists per set (6.57 per set).
Jimerson played in all 32 matches and appeared in all 122 sets in her 2017 rookie season, earning MAC Freshman of the Year and All-MAC First Team honors as well as a spot on the MAC All-Freshman Team. She led the team and ranked fourth in the MAC and 59th in the country with a .358 attack percentage (409 kills-112 errors-829 total attacks), which tied for the third-best hitting percentage in a single season in the rally scoring era and tied for fifth-best in a single season in the overall history of the program. Jimerson ranked second on the team and fourth in the MAC with 409 kills (3.35 per set) and posted double-digit kills 21 times -- including 20 or more three times. She led the team with 105 total blocks (25 solo, 80 assisted; 0.86 per set), with her 25 solo blocks tied for the seventh-most in a single season in the rally scoring era of the program. She posted five or more total blocks 10 times. Jimerson also ranked second on the team with 479 points (3.93 per set) and posted 20 or more points six times.
Jimerson, who has missed the last seven matches due to injury, is tied for fourth on the team with 19 total blocks (19 assisted; 0.95 per set) and ranks fourth with 60 kills (3.00 per set).
Stephens joins Kosiorek and Nelson as Ohio's co-captains for the 2018 season.
Kosiorek and Nelson are in their second seasons serving as team co-captains.
In addition to Kosiorek, Nelson and Stephens serving as team co-captains, junior libero/defensive specialist Meredith Howe (Grand Rapids, Mich.) is in her second season serving as the program's second-ever academic captain.
Deane Webb is in his fifth year as the head coach of the Ohio volleyball program in 2018. The Bobcats have gone 86-51 (.628) under the direction of Webb. -- including 50-14 (.781) in MAC play. Ohio won a MAC regular season title with a perfect 16-0 (1.000) league year in 2014, then captured the conference tournament title in 2015, the latter of which propelled the 'Cats to their 10th NCAA Championship appearance in program history.
The Bobcats have also excelled in the classroom under Webb. Ohio's 22 Academic All-MAC selections since Webb's arrival in Athens are the most under any head coach in the history of the Ohio volleyball program.
Webb owns an overall career coaching record of 456-267 (.631) over 21-plus seasons -- including 315-207 (.603) over 17-plus seasons at the NCAA Division I level.
On Tuesday, Webb caught up with Hromada to recap the Mizzou Invitational and preview the first weekend of conference play.
Ohio's bench features a coaching staff with both familiar and new faces. Joining Webb for a fifth season in Athens is assistant coach Maggie Couture. Rodnei Santos is in his second year as an assistant coach on Webb's staff. Laura Santos moves from the director of operations role to the volunteer assistant coach spot for her second season with the Bobcats. New to the Ohio coaching staff this year are director of operations Adam Kuntz and graduate manager/technical coordinator Andy Rynd.
The Bobcats' 29-match regular season slate is highlighted by four non-conference tournaments, 11 home matches at the Convocation Center and 16 MAC matchups. The Bobcats will battle six teams that ranked in the top 100 of the Ratings Percentage Index (RPI) in 2017 and three 2017 NCAA Championship qualifiers -- including defending national champion Nebraska, regional semifinalist Missouri and MAC archrival Miami.
As a team, the 2017 squad's 183 service aces rank as the fourth-most in the rally scoring era in program history while its 1.50 service aces per set and 4,531 attacks both rank sixth, its 1,940 digs rank seventh and its 15.90 digs per set rank 10th.
The Bobcats welcome back 13 of the 2017 team's 15 student-athletes.
In addition to Giacomazzi, Howe, Jimerson, Kosiorek, Miller, Nelson and Stephens, redshirt freshman middle blocker Dahlias Bouyer (Brownstown, Mich.), junior middle blocker Sara Januszewski (Oak Creek, Wis.), senior right side hitter Stephanie Olman (Maumee, Ohio), sophomore libero/defensive specialist Macy Reihing (Lexington, Ky.), redshirt junior outside hitter Carley Remmers (Filley, Neb.) and junior outside hitter/defensive specialist Allyson Vaughn(Glenview, Ill.) are all back for Ohio in 2018.
Bouyer, Kosiorek, Miller and Nelson all missed either all or the majority of the 2017 season due to injuries.
Kosiorek was an All-MAC Honorable Mention selection in 2016. Her 3,575 total attacks rank sixth overall in program history and fourth in the rally scoring era. Her 150 service aces are fifth overall in program history and second in the rally scoring era. She is 13 service aces away from breaking the program's career rally scoring era record. Her 0.38 service aces per set rank second in the rally scoring era in program history. Her 1,167 kills rank ninth in the rally scoring era in program history. Her 2.97 kills per set rank 10th in the rally scoring era in program history. Her 1,389.5 points rank eighth in the rally scoring era in program history. Her 3.53 points per set are seventh in the rally scoring era in program history.
Through 13 matches in 2018, Kosiorek leads Ohio with 129 kills (2.63 per set) and 30 service aces (0.61 per set). She enters this weekend ranked fourth in the country in service aces and ninth in service aces per set.
In 2016, Nelson led the team with a career-high 91 total blocks (17 solo, 74 assisted; 0.89 per set), recording five or more total blocks on a career-high seven occasions. She ranked fourth on the team with a career-high 201 kills (1.97 per set) and recorded double-digit kills on a career-high seven occasions.
Through 13 matches in 2018, Nelson ranks second on the team with 45 total blocks (three solo, 42 assisted; 0.94 per set) and third with 105 kills (2.19 per set).
Miller ranked third on the team with a career-high 49 total blocks (10 solo, 39 assisted; 0.98 per set) in 2016.
Through 13 matches in 2018, Miller leads Ohio with 50 total blocks (three solo, 47 assisted; 1.28 per set). She enters the weekend ranked fourth among MAC players in total blocks and first among MAC players in blocks per set.
With the squad plagued by injuries to key players, several Bobcats rose to the occasion to help keep Ohio competitive in 2017.
Olman ranked third on the team with a career-high 203 kills (1.95 per set) and posted double-digit kills a career-high nine times.
Januszewski totaled a career-high 158 kills (1.48 per set) to rank fourth on the team and posted double-digit kills three times. She also compiled a career-high 80 total blocks (nine solo, 71 assisted; 0.75 per set) to rank second on the team and posted five or more total blocks five times.
Vaughn ranked fourth on the team with a career-high 23 service aces (0.20 per set). She also tallied three or more service aces on two occasions.
Howe ranked fourth on the team with a career-high 275 digs (2.46 per set) and tallied double-digit digs on a career-high 15 occasions. She also ranked second on the team with a career-high 27 service aces (0.24 per set).
Remmers totaled a career-high 110 kills (1.43 per set) to rank fifth on the team and posted double-digit kills once. She also tallied a career-high 199 digs (2.58 per set) to rank fifth on the team and Posted double-digits 12 times -- including 20 or more once.
Though Giacomazzi and Jimerson earned all-conference accolades in 2017, Reihing put together a freshman campaign to remember as well. Her 484 digs led the team and ranked as the 10th-most in a single season in history and eighth-most in the rally scoring era while her 4.14 digs per set ranked as the ninth-most in a single season in the rally scoring era in program history. She tallied double-digit digs on 24 occasions -- including 20 or more nine times and 30 or more once.
Through 13 matches in 2018, Reihing leads the team with 192 digs (4.00 per set).
New to the Ohio volleyball program in 2018 are five true freshmen -- outside hitter Ila Angermeier (Kirtland, Ohio), defensive specialist Virginia Natale (Garrettsville, Ohio), outside hitter/right side hitter Lauren Park (Millersburg, Ohio), setter Kyra Slavik (Saint Charles, Ill.) and defensive specialist/setter Emily Walsh (Allen, Texas).
Angermeier was a three-year starter at Lake Catholic High School, serving Served as a senior team captain for the nationally ranked Cougars in 2017 and helping to lead the squad to a second-straight Ohio High School Athletic Association Final Four berth after an undefeated regular season. She was a Second Team All-Ohio selection and earned both First Team Division II and First Team North Coast League honors for the third year in a row in 2017.
Natale was a varsity starter and three-year letterwinner at Walsh Jesuit High School. She owned a career serving percentage of 93% and helped lead the Cavaliers to four Ohio High School Athletic Association Division I district titles (2014-17) and Division I regional runner up finishes in 2016 and '17.
Park was a four-year starter at West Holmes High School, compiling the eighth-most kills in Ohio High School Athletic Association history with 1,634 over her four-year career. She garnered First Team All-Ohio honors as both a senior in 2017 and a junior in '16 after earning Second Team honors in '15.
Park was named to the All-Ameritas Players Challenge Team after hitting .267 with 15 kills (2.14 per set).
Slavik was a three-year letterwinner at Saint Charles East High School and was an All-Illinois selection in 2017 after changing from setter to right side hitter due to team injuries, leading the Fighting Saints to their first regional win since '14 and an appearance in the sectional finals as team captain.
Slavik currently ranks second on the team with 136 assists (3.89 per set).
Walsh was a four-year letterwinner at Allen High School and Earned Honorable Mention All-District 6-6A honors as both a junior in 2016 and a senior in '17.
Ohio is 53-22 (.707) all-time against Kent State. The Bobcats and the Golden Flashes split the season series in 2017, with Ohio sweeping Kent State, 3-0 (25-23, 25-23, 25-20), on Nov. 9 at the Convo in the last meeting between the two squads.
Kent State, which was picked to place fifth in the East Division in the MAC's preseason poll, is led by seventh-year head coach Don Gromala.
Freshman outside hitter Kathryn Ellison leads the Golden Flashes with 134 kills (2.68 per set). Freshman defensive specialist/libero Erin Gardner has tallied a team-high 18 service aces (0.38 per set), which is tied for third among MAC players. She also leads the squad with 212 digs (4.42 per set). Redshirt freshman setter Alex Haffner has dished out a team-high 284 assists (8.11 per set). Junior middle blocker Myla King leads Kent State with 39 total blocks (four solo, 35 assisted; 0.95 per set) while freshman middle blocker Danie Tyson ranks second with 35 total blocks (12 solo, 23 assisted; 0.76 per set).
The Bobcats trail the all-time series with the Chippewas, 26-22. In the lone meeting of the 2017 season between Ohio and Central Michigan, the Bobcats earned a 3-0 (25-22, 25-12, 25-21) sweep of the Chippewas on Nov. 11 at the Convo.
Central Michigan is guided by third-year head coach Mike Gawlik. The Chippewas were picked to finish third in the West Division in the league's preseason poll.
Sophomore outside hitter Kalina Smith leads Central Michigan with 127 kills (2.82 per set). Freshman outside hitter Savannah Thompson has tallied a team-high 11 service aces (0.24 per set) and a team-high 126 digs (2.80 per set) in addition to ranking third on the squad with 100 kills (2.22 per set). Junior libero Megan Kern ranks second on the team with 125 digs (2.78 per set) while senior defensive specialist/outside hitter Emily Wenglikowski is third with 111 (2.47 per set). Senior setter Marissa Grant has dished out a team-high 211 assists (5.86 per set). Junior middle blocker Jayla Wesley leads the Chippewas with 27 total blocks (four solo, 23 assisted; 0.63 per set) while sophomore outside hitter Lyza Myers ranks second with 25 total blocks (two solo, 23 assisted; 0.56 per set) and freshman middle blocker Maddie Whitfield ranks third with 23 total blocks (two solo, 21 assisted; 0.53 per set). Myers also ranks second on the team with 113 kills (2.51 per set).
Before hosting the Bobcats on Saturday, Central Michigan plays host to West Division rival Eastern Michigan on Thursday.
Ohio will be back at the Convo next weekend to play host to a pair of East Division rivals, welcoming Buffalo next Friday (Sept. 28) at 7 p.m. and Akron on Saturday (Sept. 29) at 6 p.m. Both matches will be streamed live on ESPN+.


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Heritage Park will be open for cross country schooling on Saturday, November 4th. Gates open 9:00am and close to new arrivals at 2:30pm
The park has turned off water. Please pack water for your horses. The water complex currently is full due to the recent rains.
The 2017 Horse of the Year Standings through Heritage Park Horse Trials have been published. Point inquiries must be directed to Cynthia Wiseman email@example.com prior to November 15th, 2017.


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Country of Mountains Blogger Template
Free Country of Mountains Blogger Template is a nice blogger template adapted from WordPress Theme to Blogger Template with nice creative design and modern style. It's suitable travel blogs specially in Nepal
"Nepal is home to eight highest mountains in the world. The most famous is Himalayan Mountain which extends across the country from the eastern to western. People love this country of mountains and come here just to try and climb the biggest mountain in the world. Do you want to be one among them? Welcome to join us!"
Similar Templates : Your Lerisure Time at Honduras Blogger Template & Carnival In Bolivia Blogger Template
Designer & Author : Bettyduncan - http://www.nepaltravelwpthemes.net/


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image via – shutterstock.com
The best way to describe just about anything is by what color it happens to be. Whether it's the sky, a car, house, plant, animal, or a person, they are all some distinct shade of color. Even things that we cannot see, like air or certain gasses, are described as clear, invisible, or lacking color.
The same can be said about a person's aura, which is the energy that we each radiate and put off. All living things and even ordinary, inanimate objects have an aura, yet many of us cannot see them. However, whether or not you're able to see your own or other people's auras does not matter because they are with us constantly and vibrating colorfully, irregardless of how well your able to view them.
Whatever color your aura may be is a direct reflection of your life at the moment. Over time, as we go through changes and grow personally, it changes right along with us. As such, whatever color of the rainbow your aura is currently vibrating at is directly in response to all of your unconscious thoughts and desires.
There are also many different ways in which the color of our aura may be affecting us. Our behavior, perceptions, and personalities, and how others view us in terms of those types of factors, are all influenced by it in one way or another. Just think about it, the color of your aura influences your life in not only minor, but major ways as well. You probably never even knew or thought about it!
To find out what color your aura is, take this quiz, it delves deep into your unconscious to give you the answer.
What color did you get? Let us know in the comments
Please SHARE This With Family and Friends To See What Color Their Aura Is 🙂


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Print and mail outsourcing is the outsourcing of document printing and distribution.
In the world of statement processing and critical variable print customer communications, outsourcing first became popular with the largest financial services firms in the 1990s. The volume of printing and mailing and associated operational overhead on their balance sheets commanded considerable visibility. Consultants helped firms to ask the question, "What are my core competencies?" Can someone else do this better for me? The answer, unless corporations were mega print and mail experts running load-leveled operations, almost never included "print and mail" as a core competency. The answer was usually "yes".
The term "outsourcing" became very common in the print and mail business and later expanded to be very broad and inclusive of most any process by the year 2000, including offshore outsourcing, which rapidly became most synonymous with the term because of the political visibility.
But print and mail outsourcing has continued to grow with advancing technology supporting the industry since the onset of laser printing. Now color laser, digital presses and high speed full color commercial ink jet printing are coming into full production environments. "Print to mail" or "Print and mail" are now common terms for referring to the process of utilizing outside firms specializing in print and mail to handle these common production tasks.
1. Data transfer - The customer transfer files and documents that require printing and mailing. Transfer can be accomplished via email or public or private ftp upload
2. Data processing - The provider will process this data to extract key information for mailing and print file set-up for downstream automation. The provider will then perform the document formatting. (Turning data into documents).
3. Document formatting - data is formatted according to a composition rules engine that has been programmed or set up to format the data into specific design formats. In addition to design and content considerations, the documents are formatted to include barcodes that are read by the automated inserting equipment. The result is a fully composed print file ready to RIP (raster image processor) and print at the compatible print device.
3. Variable printing - each piece of the mailing can be customized to better reflect the end user. Data such as customer name, appropriate images, etc. can be modified on each printed piece. Variable printing is shown to increase response rates of direct mail.
4. Automated Envelope Inserting - totally automates the merging of personalized documents of variable lengths, auto-folds and inserts documents into an envelope.
5. USPS Mailing - mailing campaigns can be delivered to the USPS with correct postage adhered and meeting all USPS guidelines.
This largely depends on whether the business is starting with raw data or self composed print files.
1. Raw data - While the printing hardware and inserting technology to support faster, more high quality print to mail processes in the manufacturing environment has helped to drive the justification of ongoing print to mail project outsourcing, it can be a painful experience for companies to contract and integrate with a chosen provider. Large upfront expenditures on design, programming, project management and setup can be common. Processes and manufacturing processes and standards often are the driving force behind providers' programmers approach to development. At times this can conflict with design and composition considerations. Providers often provide these services for programming fees while positioning themselves as keeper of their clients' document composition rules, formatting and content. This has its pros and cons. Outsourcing the full responsibility, starting with the receipt and formatting of raw data allows companies to eliminate the responsibility for software and design tools used in this process and allows them to focus on their core competencies. This can be very desirable, especially if there are no internal designers available.
2. Print files - The sophistication and ease of use of today's document composition software has led to many businesses maintaining their own document composition. The main advantages of this are control, speed of change management, and cost control. Online services compliment this software with ready-to-use print to mail drivers and options. Online print to mail picks up where document composition leaves off, and can be implemented quickly and inexpensively. This new generation of online print to mail services is enabling business to maintain control of their document content while still eliminating the in house print to mail activities and costs. Some examples are [1] MailXstream, and [2] MooreWallaceVPO. These services are standardized on Adobe PDF file format making their services compatible with virtually any windows generated print files.
According to industry estimates, printing accounts for 1-3% of a company's' overall budget. When companies keep printing in-house, most organizations are not able to realize the full costs of printing. Outsourcing provides additional transparency and allows for a tighter controlled budgets and can reduce expenses saving hundreds, if not thousands of dollars per year. Additionally, the equipment and expertise required to create professional and customized pieces of collateral are often expenses that many small to mid-size firms can no longer afford. By outsourcing, some estimates show that companies can save approximately 30% on their annual printing budgets.
Globalization, Business process outsourcing, Canada, Linguistics, Offshoring
FedEx, New York City, Benjamin Franklin, Washington, D.C., United Parcel Service


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About this event:
Created by Dean Mortlock
High Street, Burnham-On-Sea
Burnham-On-Sea Farmers' Market is a very lively and friendly market held on the last Friday of the month right in the centre of the town. There's a good selection of local traders selling delicious foods and stunning drinks.
BURNHAM-ON-SEA – High Street
Last Friday of the month / 9am-1pm


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As Told By Ginger Episode 33 - Family Therapy
You will Watch As Told By Ginger Episode 33 online for free episodes with HQ / high quality. Stream cartoons As Told By Ginger Episode 33 Family Therapy Macie's parents forget about her thirteenth birthday. When Ginger tells Bobby and Bobbie Lightfoot, they feel extremely guilty and ensure that they make it up to Macie. They begin to treat her like a four-year-old, showering her with baby toys and hosting her birthday party at a petting zoo. At the end, Macie decides that while she's had fun catching up on lost childhood, she is a teenager now and her parents should treat her as such. Meanwhile, Carl hides in fear from an escaped naked mole rat that crawled up his pants leg, but later he decides to find it, losing his fear when he discovers that it's even more frightened of him.


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Professor Carolyn Bertozzi's research interests span the disciplines of chemistry and biology with an emphasis on studies of cell surface sugars important to human health and disease. Her research group profiles changes in cell surface glycosylation associated with cancer, inflammation and bacterial infection, and uses this information to develop new diagnostic and therapeutic approaches, most recently in the area of immuno-oncology.
Dr. Bertozzi completed her undergraduate degree in Chemistry at Harvard University and her Ph.D. at UC Berkeley, focusing on the chemical synthesis of oligosaccharide analogs. During postdoctoral work at UC San Francisco, she studied the activity of endothelial oligosaccharides in promoting cell adhesion at sites of inflammation. She joined the UC Berkeley faculty in 1996. A Howard Hughes Medical Institute Investigator since 2000, she came to Stanford University in June 2015, among the first faculty to join the interdisciplinary institute ChEM-H (Chemistry, Engineering & Medicine for Human Health). Named a MacArthur Fellow in 1999, Dr. Bertozzi has received many awards for her dedication to chemistry, and to training a new generation of scientists fluent in both chemistry and biology. She has been elected to the Institute of Medicine, National Academy of Sciences, and American Academy of Arts and Sciences; and received the Lemelson-MIT Prize, the Heinrich Wieland Prize, and the ACS Award in Pure Chemistry, among many others. Her efforts in undergraduate education have earned the UC Berkeley Distinguished Teaching Award and the Donald Sterling Noyce Prize for Excellence in Undergraduate Teaching.
Today, the Bertozzi Group at Stanford studies the glycobiology underlying diseases such as cancer, inflammatory disorders such as arthritis, and infectious diseases such as tuberculosis. The work has advanced understanding of cell surface oligosaccharides involved in cell recognition and inter-cellular communication.
Dr. Bertozzi's lab also develops new methods to perform controlled chemical reactions within living systems. The group has developed new tools for studying glycans in living systems, and more recently nanotechnologies for probing biological systems. Such "bioorthogonal" chemistries enable manipulation of biomolecules in their living environment.
Several of the technologies developed in the Bertozzi lab have been adapted for commercial use. Actively engaged with several biotechnology start-ups, Dr. Bertozzi founded Redwood Bioscience of Emeryville, California, and has served on the research advisory board of GlaxoSmithKline.
Academic Appointments
Professor, Chemistry
Professor (By courtesy), Radiology
Professor (By courtesy), Chemical and Systems Biology
Member, Bio-X
Member, Stanford Cancer Institute
Faculty Fellow, Stanford ChEM-H
Member, Wu Tsai Neurosciences Institute
Administrative Appointments
Investigator, Howard Hughes Medical Institute (2000 - Present)
Honors & Awards
Arthur C. Cope Award, American Chemical Society (2017)
National Academy of Sciences Award in the Chemical Sciences, National Academy of Sciences (2016)
Ernest Orlando Lawrence Award, U.S. Department of Energy (2015)
Heinrich Wieland Prize, Heinrich Wieland Prize (2012)
Lemelson-MIT Prize, Massachusetts Institute of Technology (2010)
Ernst Schering Prize, Ernst Schering Research Foundation (2007)
Distinguished Teaching Award, UC Berkeley College of Chemistry (2001)
Award in Pure Chemistry, American Chemical Society (2001)
MacArthur Foundation "Genius" Award, MacArthur Foundation (1999)
Arthur C. Cope Scholar Award, American Chemical Society (1999)
Honorary Degree, Freie University Berlin (2014)
Honorary Doctorate Degree, Duke University (2014)
Hans Bloemendal Award, Radboud Univ. Nijmegen (2013)
Honorary Doctorate Degree, Brown University (2012)
Tetrahedron Young Investigator Award, Executive Board of Editors and the Publisher of Tetrahedron Publications (2011)
Albert Hofmann Medal, U. Zurich (2009)
Harrison Howe Award, Rochester Section, American Chemical Society (2009)
W. H. Nichols Award, New York Section, American Chemical Society (2009)
Li Ka Shing Women in Science Award, Li Ka Shing Foundation Women in Science Program (2008)
Roy L. Whistler International Award in Carbohydrate Chemistry, International Carbohydrate Organization (2008)
Willard Gibbs Medal, Chicago Section, American Chemical Society (2008)
T.Z. and Irmgard Chu Distinguished Professorship in Chemistry, UC Berkeley (2005-14)
Havinga Medal, U. Leiden (2005)
Agnes Fay Morgan Research Award, Iota Sigma Pi (2004)
Fellow, American Association for the Advancement of Science (2002)
Irving Sigal Young Investigator Award, Protein Society (2002)
Donald Sterling Noyce Prize for Excellence in Undergraduate Teaching, UC Berkeley College of Chemistry (2001)
Department of Chemistry Teaching Award, UC Berkeley (2000)
Merck Academic Development Program Award, Merck (2000)
Presidential Early Career Award in Science and Engineering (PECASE), The U.S. White House (2000)
Camille Dreyfus Teacher-Scholar Award, Camille and Henry Dreyfus Foundation (1999)
Beckman Young Investigator Award, Arnold and Mabel Beckman Foundation (1998)
Glaxo Wellcome Scholar, Glaxo Wellcome (1998)
Prytanean Faculty Award, Prytanean Women's Honor Society, UC Berkeley (1998)
Research Innovation Award, Research Corporation (1998)
Young Investigator Award, Office of Naval Research (1998)
Horace S. Isbell Award in Carbohydrate Chemistry, American Chemical Society (1997)
New Investigator Award in Pharmacology, Burroughs Wellcome (1997)
Sloan Research Fellow, Alfred P. Sloan Foundation (1997)
Pew Scholars Award in the Biomedical Sciences, Pew Charitable Trusts (1996)
Young Investigator Award, Exxon Education Fund (1996)
Dreyfus New Faculty Award, Camille and Henry Dreyfus Foundation (1995)
Boards, Advisory Committees, Professional Organizations
Member, National Academy of Inventors (2013 - Present)
Member, Institute of Medicine (2011 - Present)
Member, German Academy of Sciences Leopoldina (2008 - Present)
Member, National Academy of Sciences (2005 - Present)
Member, American Academy of Arts and Sciences (2003 - Present)
Chair, Scientific Advisory Board, Redwood Bioscience
Board Member, Board of Scientific Counslors, Broad Institutue
Board Member, Catalent Biologics Board
Board Member, Research Advisory Baord, Glaxo Smithkline
Professional Education
Postdoc, UC San Francisco, Immunology
PhD, UC Berkeley, Chemistry (1993)
AB, Harvard University, Chemistry (1988)
Additional Info
2019-20 Courses
- Department Colloquium
CHEM 300 (Aut, Win, Spr)
- Organic Chemistry Seminar
CHEM 329 (Aut, Win, Spr)
- The Chemical Principles of Life II
CHEM 143 (Spr)
- Therapeutic Science at the Chemistry - Biology Interface
CHEM 281 (Win)
Independent Studies (12)
- Advanced Undergraduate Research
CHEM 190 (Aut, Win, Spr, Sum)
- Directed Instruction/Reading
CHEM 90 (Aut, Win, Spr, Sum)
- Directed Investigation
BIOE 392 (Aut, Win, Spr, Sum)
- Directed Reading in Stem Cell Biology and Regenerative Medicine
STEMREM 299 (Win, Spr)
- Graduate Research
IMMUNOL 399 (Aut, Win, Spr, Sum)
- Graduate Research
STEMREM 399 (Aut, Win, Spr)
- Medical Scholars Research
STEMREM 370 (Aut, Win, Spr)
- Out-of-Department Advanced Research Laboratory in Experimental Biology
BIO 199X (Aut, Win, Spr)
- Out-of-Department Graduate Research
BIO 300X (Aut, Win, Spr, Sum)
- Research and Special Advanced Work
CHEM 200 (Aut, Win, Spr, Sum)
- Research in Chemistry
CHEM 301 (Aut, Win, Spr, Sum)
- Undergraduate Research
STEMREM 199 (Aut, Win, Spr)
- Advanced Undergraduate Research
Prior Year Courses
2018-19 Courses
- Department Colloquium
CHEM 300 (Aut, Win, Spr)
- Organic Chemistry Seminar
CHEM 229 (Aut, Win, Spr)
- Organic Chemistry of Bioactive Molecules
CHEM 35 (Aut)
- Therapeutic Science at the Chemistry - Biology Interface
CHEM 227 (Spr)
2017-18 Courses
- Department Colloquium
CHEM 300 (Aut, Win, Spr)
- Organic Chemistry Seminar
CHEM 229 (Aut, Win, Spr)
- Organic Chemistry of Bioactive Molecules
CHEM 35 (Aut)
- Therapeutic Science at the Chemistry - Biology Interface
CHEM 227 (Spr)
2016-17 Courses
- Department Colloquium
CHEM 300 (Aut, Win, Spr)
- Exploring Chemical Research at Stanford
CHEM 111 (Win)
- Organic Chemistry Seminar
CHEM 229 (Aut, Win, Spr)
- Therapeutic Science at the Chemistry - Biology Interface
CHEM 227 (Spr)
- Department Colloquium
Stanford Advisees
Doctoral Dissertation Reader (AC)
Kaustabh Basu, Doreen Chan, Tingting Dai, Kevin Erazo Castillo, Holly Hajare, Shuo Han, Clayton Hardman, Tim Horton, Veronica Li, Kang Yong Loh, Payton Marshall, Jared Nesvet, Steven Shuken, Payton Weidenbacher, Andrew Yang, Alex Yoshikawa
Postdoctoral Faculty Sponsor
Steven Banik, Joe Buonomo, CJ Cambier, Ryan Flynn, Rishi Kulkarni, Nicholas Riley, Jessica Stark, Simon Wisnovsky
Doctoral Dissertation Advisor (AC)
Green Ahn, Corleone Delaveris, Justin Donnelly, Giovanni Forcina, Melissa Gray, Kayvon Pedram, Egan Peltan, Judy Shon, Wilson Sinclair, Benjie Smith, Gabby Tender, Shivam Verma
Doctoral Dissertation Co-Advisor (AC)
Laura Handy, Michael Hollander
Doctoral (Program)
Giovanni Forcina
All Publications
Precision glycocalyx editing as a strategy for cancer immunotherapy.
Proceedings of the National Academy of Sciences of the United States of America
2016; 113 (37): 10304-10309
Cell surface sialosides constitute a central axis of immune modulation that is exploited by tumors to evade both innate and adaptive immune destruction. Therapeutic strategies that target tumor-associated sialosides may therefore potentiate antitumor immunity. Here, we report the development of antibody-sialidase conjugates that enhance tumor cell susceptibility to antibody-dependent cell-mediated cytotoxicity (ADCC) by selective desialylation of the tumor cell glycocalyx. We chemically fused a recombinant sialidase to the human epidermal growth factor receptor 2 (HER2)-specific antibody trastuzumab through a C-terminal aldehyde tag. The antibody-sialidase conjugate desialylated tumor cells in a HER2-dependent manner, reduced binding by natural killer (NK) cell inhibitory sialic acid-binding Ig-like lectin (Siglec) receptors, and enhanced binding to the NK-activating receptor natural killer group 2D (NKG2D). Sialidase conjugation to trastuzumab enhanced ADCC against tumor cells expressing moderate levels of HER2, suggesting a therapeutic strategy for cancer patients with lower HER2 levels or inherent trastuzumab resistance. Precision glycocalyx editing with antibody-enzyme conjugates is therefore a promising avenue for cancer immune therapy.
View details for DOI 10.1073/pnas.1608069113
View details for PubMedID 27551071
Chemically tunable mucin chimeras assembled on living cells
2015; 112 (41): 12574-12579
View details for DOI 10.1073/pnas.1516127112
View details for PubMedID 26420872
CalFluors: A Universal Motif for Fluorogenic Azide Probes across the Visible Spectrum
2015; 137 (22): 7145-7151
Fluorescent bioorthogonal smart probes across the visible spectrum will enable sensitive visualization of metabolically labeled molecules in biological systems. Here we present a unified design, based on the principle of photoinduced electron transfer, to access a panel of highly fluorogenic azide probes that are activated by conversion to the corresponding triazoles via click chemistry. Termed the CalFluors, these probes possess emission maxima that range from green to far red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. We used the CalFluor probes to image various alkyne-labeled biomolecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices.
View details for DOI 10.1021/jacs.5b02383
View details for Web of Science ID 000356322300038
View details for PubMedID 25902190
View details for PubMedCentralID PMC4487548
Isotope-targeted glycoproteomics (IsoTaG): a mass-independent platform for intact N- and O-glycopeptide discovery and analysis
2015; 12 (6): 561-?
Protein glycosylation is a heterogeneous post-translational modification (PTM) that plays an essential role in biological regulation. However, the diversity found in glycoproteins has undermined efforts to describe the intact glycoproteome via mass spectrometry (MS). We present IsoTaG, a mass-independent chemical glycoproteomics platform for characterization of intact, metabolically labeled glycopeptides at the whole-proteome scale. In IsoTaG, metabolic labeling of the glycoproteome is combined with (i) chemical enrichment and isotopic recoding of glycopeptides to select peptides for targeted glycoproteomics using directed MS and (ii) mass-independent assignment of intact glycopeptides. We structurally assigned 32 N-glycopeptides and over 500 intact and fully elaborated O-glycopeptides from 250 proteins across three human cancer cell lines and also discovered unexpected peptide sequence polymorphisms (pSPs). The IsoTaG platform is broadly applicable to the discovery of PTM sites that are amenable to chemical labeling, as well as previously unknown protein isoforms including pSPs.
View details for DOI 10.1038/nmeth.3366
View details for Web of Science ID 000355248100027
View details for PubMedID 25894945
The cancer glycocalyx mechanically primes integrin-mediated growth and survival
2014; 511 (7509): 319-?
Malignancy is associated with altered expression of glycans and glycoproteins that contribute to the cellular glycocalyx. We constructed a glycoprotein expression signature, which revealed that metastatic tumours upregulate expression of bulky glycoproteins. A computational model predicted that these glycoproteins would influence transmembrane receptor spatial organization and function. We tested this prediction by investigating whether bulky glycoproteins in the glycocalyx promote a tumour phenotype in human cells by increasing integrin adhesion and signalling. Our data revealed that a bulky glycocalyx facilitates integrin clustering by funnelling active integrins into adhesions and altering integrin state by applying tension to matrix-bound integrins, independent of actomyosin contractility. Expression of large tumour-associated glycoproteins in non-transformed mammary cells promoted focal adhesion assembly and facilitated integrin-dependent growth factor signalling to support cell growth and survival. Clinical studies revealed that large glycoproteins are abundantly expressed on circulating tumour cells from patients with advanced disease. Thus, a bulky glycocalyx is a feature of tumour cells that could foster metastasis by mechanically enhancing cell-surface receptor function.
View details for DOI 10.1038/nature13535
View details for Web of Science ID 000338992200029
View details for PubMedID 25030168
Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes
2014; 111 (15): 5456-5461
Fluorescent probes designed for activation by bioorthogonal chemistry have enabled the visualization of biomolecules in living systems. Such activatable probes with near-infrared (NIR) emission would be ideal for in vivo imaging but have proven difficult to engineer. We present the development of NIR fluorogenic azide probes based on the Si-rhodamine scaffold that undergo a fluorescence enhancement of up to 48-fold upon reaction with terminal or strained alkynes. We used the probes for mammalian cell surface imaging and, in conjunction with a new class of cyclooctyne D-amino acids, for visualization of bacterial peptidoglycan without the need to wash away unreacted probe.
View details for DOI 10.1073/pnas.1322727111
View details for Web of Science ID 000334288600021
View details for PubMedID 24706769
Glycocalyx engineering reveals a Siglec-based mechanism for NK cell immunoevasion
The increase of cell surface sialic acid is a characteristic shared by many tumor types. A correlation between hypersialylation and immunoprotection has been observed, but few hypotheses have provided a mechanistic understanding of this immunosuppressive phenomenon. Here, we show that increasing sialylated glycans on cancer cells inhibits human natural killer (NK) cell activation through the recruitment of sialic acid-binding immunoglobulin-like lectin 7 (Siglec-7). Key to these findings was the use of glycopolymers end-functionalized with phospholipids, which enable the introduction of synthetically defined glycans onto cancer cell surfaces. Remodeling the sialylation status of cancer cells affected the susceptibility to NK cell cytotoxicity via Siglec-7 engagement in a variety of tumor types. These results support a model in which hypersialylation offers a selective advantage to tumor cells under pressure from NK immunosurveillance by increasing Siglec ligands. We also exploited this finding to protect allogeneic and xenogeneic primary cells from NK-mediated killing, suggesting the potential of Siglecs as therapeutic targets in cell transplant therapy.
View details for DOI 10.1038/NCHEMBIO.1388
View details for Web of Science ID 000328854900013
View details for PubMedID 24292068
Osmosensory signaling in Mycobacterium tuberculosis mediated by a eukaryotic-like Ser/Thr protein kinase
Bacteria are able to adapt to dramatically different microenvironments, but in many organisms, the signaling pathways, transcriptional programs, and downstream physiological changes involved in adaptation are not well-understood. Here, we discovered that osmotic stress stimulates a signaling network in Mycobacterium tuberculosis regulated by the eukaryotic-like receptor Ser/Thr protein kinase PknD. Expression of the PknD substrate Rv0516c was highly induced by osmotic stress. Furthermore, Rv0516c disruption modified peptidoglycan thickness, enhanced antibiotic resistance, and activated genes in the regulon of the alternative σ-factor SigF. Phosphorylation of Rv0516c regulated the abundance of EspA, a virulence-associated substrate of the type VII ESX-1 secretion system. These findings identify an osmosensory pathway orchestrated by PknD, Rv0516c, and SigF that enables adaptation to osmotic stress through cell wall remodeling and virulence factor production. Given the widespread occurrence of eukaryotic-like Ser/Thr protein kinases in bacteria, these proteins may play a broad role in bacterial osmosensing.
View details for DOI 10.1073/pnas.1321205110
View details for Web of Science ID 000328858800008
View details for PubMedID 24309377
Imaging the Glycosylation State of Cell Surface Glycoproteins by Two-Photon Fluorescence Lifetime Imaging Microscopy
2013; 52 (52): 14045-14049
View details for DOI 10.1002/anie.201307512
View details for Web of Science ID 000328531100027
View details for PubMedID 24259491
View details for PubMedCentralID PMC3920747
D-Amino Acid Chemical Reporters Reveal Peptidoglycan Dynamics of an Intracellular Pathogen
2013; 8 (3): 500-505
Peptidoglycan (PG) is an essential component of the bacterial cell wall. Although experiments with organisms in vitro have yielded a wealth of information on PG synthesis and maturation, it is unclear how these studies translate to bacteria replicating within host cells. We report a chemical approach for probing PG in vivo via metabolic labeling and bioorthogonal chemistry. A wide variety of bacterial species incorporated azide and alkyne-functionalized d-alanine into their cell walls, which we visualized by covalent reaction with click chemistry probes. The d-alanine analogues were specifically incorporated into nascent PG of the intracellular pathogen Listeria monocytogenes both in vitro and during macrophage infection. Metabolic incorporation of d-alanine derivatives and click chemistry detection constitute a facile, modular platform that facilitates unprecedented spatial and temporal resolution of PG dynamics in vivo.
View details for DOI 10.1021/cb3004995
View details for Web of Science ID 000316375500003
View details for PubMedID 23240806
View details for PubMedCentralID PMC3601600
A Pictet-Spengler ligation for protein chemical modification
2013; 110 (1): 46-51
Aldehyde- and ketone-functionalized proteins are appealing substrates for the development of chemically modified biotherapeutics and protein-based materials. Their reactive carbonyl groups are typically conjugated with α-effect nucleophiles, such as substituted hydrazines and alkoxyamines, to generate hydrazones and oximes, respectively. However, the resulting C=N linkages are susceptible to hydrolysis under physiologically relevant conditions, which limits the utility of such conjugates in biological systems. Here we introduce a Pictet-Spengler ligation that is based on the classic Pictet-Spengler reaction of aldehydes and tryptamine nucleophiles. The ligation exploits the bioorthogonal reaction of aldehydes and alkoxyamines to form an intermediate oxyiminium ion; this intermediate undergoes intramolecular C-C bond formation with an indole nucleophile to form an oxacarboline product that is hydrolytically stable. We used the reaction for site-specific chemical modification of glyoxyl- and formylglycine-functionalized proteins, including an aldehyde-tagged variant of the therapeutic monoclonal antibody Herceptin. In conjunction with techniques for site-specific introduction of aldehydes into proteins, the Pictet-Spengler ligation offers a means to generate stable bioconjugates for medical and materials applications.
View details for DOI 10.1073/pnas.1213186110
View details for Web of Science ID 000313630300024
View details for PubMedID 23237853
Reactivity of Biarylazacyclooctynones in Copper-Free Click Chemistry
2012; 134 (22): 9199-9208
The 1,3-dipolar cycloaddition of cyclooctynes with azides, also called "copper-free click chemistry", is a bioorthogonal reaction with widespread applications in biological discovery. The kinetics of this reaction are of paramount importance for studies of dynamic processes, particularly in living subjects. Here we performed a systematic analysis of the effects of strain and electronics on the reactivity of cyclooctynes with azides through both experimental measurements and computational studies using a density functional theory (DFT) distortion/interaction transition state model. In particular, we focused on biarylazacyclooctynone (BARAC) because it reacts with azides faster than any other reported cyclooctyne and its modular synthesis facilitated rapid access to analogues. We found that substituents on BARAC's aryl rings can alter the calculated transition state interaction energy of the cycloaddition through electronic effects or the calculated distortion energy through steric effects. Experimental data confirmed that electronic perturbation of BARAC's aryl rings has a modest effect on reaction rate, whereas steric hindrance in the transition state can significantly retard the reaction. Drawing on these results, we analyzed the relationship between alkyne bond angles, which we determined using X-ray crystallography, and reactivity, quantified by experimental second-order rate constants, for a range of cyclooctynes. Our results suggest a correlation between decreased alkyne bond angle and increased cyclooctyne reactivity. Finally, we obtained structural and computational data that revealed the relationship between the conformation of BARAC's central lactam and compound reactivity. Collectively, these results indicate that the distortion/interaction model combined with bond angle analysis will enable predictions of cyclooctyne reactivity and the rational design of new reagents for copper-free click chemistry.
View details for DOI 10.1021/ja3000936
View details for Web of Science ID 000304837800041
View details for PubMedID 22553995
Mapping Yeast N-Glycosites with Isotopically Recoded Glycans
2012; 11 (6)
Asparagine-linked glycosylation is a common post-translational modification of proteins; in addition to participating in key macromolecular interactions, N-glycans contribute to protein folding, trafficking, and stability. Despite their importance, few N-glycosites have been experimentally mapped in the Saccharomyces cerevisiae proteome. Factors including glycan heterogeneity, low abundance, and low occupancy can complicate site mapping. Here, we report a novel mass spectrometry-based strategy for detection of N-glycosites in the yeast proteome. Our method imparts N-glycopeptide mass envelopes with a pattern that is computationally distinguishable from background ions. Isotopic recoding is achieved via metabolic incorporation of a defined mixture of N-acetylglucosamine isotopologs into N-glycans. Peptides bearing the recoded envelopes are specifically targeted for fragmentation, facilitating high confidence site mapping. This strategy requires no chemical modification of the N-glycans or stringent sample enrichment. Further, enzymatically simplified N-glycans are preserved on peptides. Using this approach, we identify 133 N-glycosites spanning 58 proteins, nearly doubling the number of experimentally observed N-glycosites in the yeast proteome.
View details for DOI 10.1074/mcp.M111.015339
View details for Web of Science ID 000306408500017
View details for PubMedID 22261724
Elucidation and Chemical Modulation of Sulfolipid-1 Biosynthesis in Mycobacterium tuberculosis
2012; 287 (11): 7990-8000
Mycobacterium tuberculosis possesses unique cell-surface lipids that have been implicated in virulence. One of the most abundant is sulfolipid-1 (SL-1), a tetraacyl-sulfotrehalose glycolipid. Although the early steps in SL-1 biosynthesis are known, the machinery underlying the final acylation reactions is not understood. We provide genetic and biochemical evidence for the activities of two proteins, Chp1 and Sap (corresponding to gene loci rv3822 and rv3821), that complete this pathway. The membrane-associated acyltransferase Chp1 accepts a synthetic diacyl sulfolipid and transfers an acyl group regioselectively from one donor substrate molecule to a second acceptor molecule in two successive reactions to yield a tetraacylated product. Chp1 is fully active in vitro, but in M. tuberculosis, its function is potentiated by the previously identified sulfolipid transporter MmpL8. We also show that the integral membrane protein Sap and MmpL8 are both essential for sulfolipid transport. Finally, the lipase inhibitor tetrahydrolipstatin disrupts Chp1 activity in M. tuberculosis, suggesting an avenue for perturbing SL-1 biosynthesis in vivo. These data complete the SL-1 biosynthetic pathway and corroborate a model in which lipid biosynthesis and transmembrane transport are coupled at the membrane-cytosol interface through the activity of multiple proteins, possibly as a macromolecular complex.
View details for DOI 10.1074/jbc.M111.315473
View details for Web of Science ID 000301349400015
View details for PubMedID 22194604
Synthesis of Heterobifunctional Protein Fusions Using Copper-Free Click Chemistry and the Aldehyde Tag
2012; 51 (17): 4161-4165
View details for DOI 10.1002/anie.201108130
View details for Web of Science ID 000303001000032
View details for PubMedID 22407566
A Bioorthogonal Quadricyclane Ligation
2011; 133 (44): 17570-17573
New additions to the bioorthogonal chemistry compendium can advance biological research by enabling multiplexed analysis of biomolecules in complex systems. Here we introduce the quadricyclane ligation, a new bioorthogonal reaction between the highly strained hydrocarbon quadricyclane and Ni bis(dithiolene) reagents. This reaction has a second-order rate constant of 0.25 M(-1) s(-1), on par with fast bioorthogonal reactions of azides, and proceeds readily in aqueous environments. Ni bis(dithiolene) probes selectively labeled quadricyclane-modified bovine serum albumin, even in the presence of cell lysate. We have demonstrated that the quadricyclane ligation is compatible with, and orthogonal to, strain-promoted azide-alkyne cycloaddition and oxime ligation chemistries by performing all three reactions in one pot on differentially functionalized protein substrates. The quadricyclane ligation joins a small but growing list of tools for the selective covalent modification of biomolecules.
View details for DOI 10.1021/ja2072934
View details for Web of Science ID 000296312200014
View details for PubMedID 21962173
From Mechanism to Mouse: A Tale of Two Bioorthogonal Reactions
2011; 44 (9): 666-676
Bioorthogonal reactions are chemical reactions that neither interact with nor interfere with a biological system. The participating functional groups must be inert to biological moieties, must selectively reactive with each other under biocompatible conditions, and, for in vivo applications, must be nontoxic to cells and organisms. Additionally, it is helpful if one reactive group is small and therefore minimally perturbing of a biomolecule into which it has been introduced either chemically or biosynthetically. Examples from the past decade suggest that a promising strategy for bioorthogonal reaction development begins with an analysis of functional group and reactivity space outside those defined by Nature. Issues such as stability of reactants and products (particularly in water), kinetics, and unwanted side reactivity with biofunctionalities must be addressed, ideally guided by detailed mechanistic studies. Finally, the reaction must be tested in a variety of environments, escalating from aqueous media to biomolecule solutions to cultured cells and, for the most optimized transformations, to live organisms. Work in our laboratory led to the development of two bioorthogonal transformations that exploit the azide as a small, abiotic, and bioinert reaction partner: the Staudinger ligation and strain-promoted azide-alkyne cycloaddition. The Staudinger ligation is based on the classic Staudinger reduction of azides with triarylphosphines first reported in 1919. In the ligation reaction, the intermediate aza-ylide undergoes intramolecular reaction with an ester, forming an amide bond faster than aza-ylide hydrolysis would otherwise occur in water. The Staudinger ligation is highly selective and reliably forms its product in environs as demanding as live mice. However, the Staudinger ligation has some liabilities, such as the propensity of phosphine reagents to undergo air oxidation and the relatively slow kinetics of the reaction. The Staudinger ligation takes advantage of the electrophilicity of the azide; however, the azide can also participate in cycloaddition reactions. In 1961, Wittig and Krebs noted that the strained, cyclic alkyne cyclooctyne reacts violently when combined neat with phenyl azide, forming a triazole product by 1,3-dipolar cycloaddition. This observation stood in stark contrast to the slow kinetics associated with 1,3-dipolar cycloaddition of azides with unstrained, linear alkynes, the conventional Huisgen process. Notably, the reaction of azides with terminal alkynes can be accelerated dramatically by copper catalysis (this highly popular Cu-catalyzed azide-alkyne cycloaddition (CuAAC) is a quintessential "click" reaction). However, the copper catalysts are too cytotoxic for long-term exposure with live cells or organisms. Thus, for applications of bioorthogonal chemistry in living systems, we built upon Wittig and Krebs' observation with the design of cyclooctyne reagents that react rapidly and selectively with biomolecule-associated azides. This strain-promoted azide-alkyne cycloaddition is often referred to as "Cu-free click chemistry". Mechanistic and theoretical studies inspired the design of a series of cyclooctyne compounds bearing fluorine substituents, fused rings, and judiciously situated heteroatoms, with the goals of optimizing azide cycloaddition kinetics, stability, solubility, and pharmacokinetic properties. Cyclooctyne reagents have now been used for labeling azide-modified biomolecules on cultured cells and in live Caenorhabditis elegans, zebrafish, and mice. As this special issue testifies, the field of bioorthogonal chemistry is firmly established as a challenging frontier of reaction methodology and an important new instrument for biological discovery. The above reactions, as well as several newcomers with bioorthogonal attributes, have enabled the high-precision chemical modification of biomolecules in vitro, as well as real-time visualization of molecules and processes in cells and live organisms. The consequence is an impressive body of new knowledge and technology, amassed using a relatively small bioorthogonal reaction compendium. Expansion of this toolkit, an effort that is already well underway, is an important objective for chemists and biologists alike.
View details for DOI 10.1021/ar200148z
View details for Web of Science ID 000296075300003
View details for PubMedID 21838330
Isotopic Signature Transfer and Mass Pattern Prediction (IsoStamp): An Enabling Technique for Chemically-Directed Proteomics
2011; 6 (8): 829-836
Directed proteomics applies mass spectrometry analysis to a subset of information-rich proteins. Here we describe a method for targeting select proteins by chemical modification with a tag that imparts a distinct isotopic signature detectable in a full-scan mass spectrum. Termed isotopic signature transfer and mass pattern prediction (IsoStamp), the technique exploits the perturbing effects of a dibrominated chemical tag on a peptide's mass envelope, which can be detected with high sensitivity and fidelity using a computational method. Applying IsoStamp, we were able to detect femtomole quantities of a single tagged protein from total mammalian cell lysates at signal-to-noise ratios as low as 2.5:1. To identify a tagged-peptide's sequence, we performed an inclusion list-driven shotgun proteomics experiment where peptides bearing a recoded mass envelope were targeted for fragmentation, allowing for direct site mapping. Using this approach, femtomole quantities of several targeted peptides were identified in total mammalian cell lysate, while traditional data-dependent methods were unable to identify as many peptides. Additionally, the isotopic signature imparted by the dibromide tag was detectable on a 12-kDa protein, suggesting applications in identifying large peptide fragments, such as those containing multiple or large posttranslational modifications (e.g., glycosylation). IsoStamp has the potential to enhance any proteomics platform that employs chemical labeling for targeted protein identification, including isotope coded affinity tagging, isobaric tagging for relative and absolute quantitation, and chemical tagging strategies for posttranslational modification.
View details for DOI 10.1021/cb100338x
View details for Web of Science ID 000294081900008
View details for PubMedID 21604797
Metabolic cross-talk allows labeling of O-linked beta-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway
2011; 108 (8): 3141-3146
Hundreds of mammalian nuclear and cytoplasmic proteins are reversibly glycosylated by O-linked β-N-acetylglucosamine (O-GlcNAc) to regulate their function, localization, and stability. Despite its broad functional significance, the dynamic and posttranslational nature of O-GlcNAc signaling makes it challenging to study using traditional molecular and cell biological techniques alone. Here, we report that metabolic cross-talk between the N-acetylgalactosamine salvage and O-GlcNAcylation pathways can be exploited for the tagging and identification of O-GlcNAcylated proteins. We found that N-azidoacetylgalactosamine (GalNAz) is converted by endogenous mammalian biosynthetic enzymes to UDP-GalNAz and then epimerized to UDP-N-azidoacetylglucosamine (GlcNAz). O-GlcNAc transferase accepts UDP-GlcNAz as a nucleotide-sugar donor, appending an azidosugar onto its native substrates, which can then be detected by covalent labeling using azide-reactive chemical probes. In a proof-of-principle proteomics experiment, we used metabolic GalNAz labeling of human cells and a bioorthogonal chemical probe to affinity-purify and identify numerous O-GlcNAcylated proteins. Our work provides a blueprint for a wide variety of future chemical approaches to identify, visualize, and characterize dynamic O-GlcNAc signaling.
View details for DOI 10.1073/pnas.1010045108
View details for Web of Science ID 000287580400017
View details for PubMedID 21300897
In vivo imaging of membrane-associated glycans in developing zebrafish
2008; 320 (5876): 664-667
Glycans are attractive targets for molecular imaging but have been inaccessible because of their incompatibility with genetically encoded reporters. We demonstrated the noninvasive imaging of glycans in live developing zebrafish, using a chemical reporter strategy. Zebrafish embryos were treated with an unnatural sugar to metabolically label their cell-surface glycans with azides. Subsequently, the embryos were reacted with fluorophore conjugates by means of copper-free click chemistry, enabling the visualization of glycans in vivo at subcellular resolution during development. At 60 hours after fertilization, we observed an increase in de novo glycan biosynthesis in the jaw region, pectoral fins, and olfactory organs. Using a multicolor detection strategy, we performed a spatiotemporal analysis of glycan expression and trafficking and identified patterns that would be undetectable with conventional molecular imaging approaches.
View details for DOI 10.1126/science.1155106
View details for Web of Science ID 000255454300046
View details for PubMedID 18451302


text:
Last year, my first year in the running, I took second place, earning a silver medal in the Canadian category. This year I have been knocked down to third place, taking bronze.
I am THRILLED that the genealogist that outshone me this year is also a Canadian.
Congratulations to Lorine McGinnis Schulz, author of Olivetree Genealogy: http://www.olivetreegenealogy.com/index.shtml
Lorine has worked tirelessly for a number of years to provide free resources for genealogy research. Earlier this year, Lorine provided a platform to encourage and mentor new genealogy bloggers.
Well deserved, Lorine!
And thank you, John, for once again providing a way for those in the genealogy community to shine!


text:
Fashion Management University of the Arts London Degree level: Undergraduate
Course summary
TEF Silver
University of the Arts London has opted into the TEF and received a Silver award.
BSc (Hons) Fashion Management is for students who are interested in business, business numeracy and quantitative skills and management within the context of the fashion industry. The fashion industry is complex, global and highly competitive. It requires graduates who can respond to changing challenges and opportunities. This course will equip you with the relevant professional, academic, communication and business skills to tackle these challenges and exploit any opportunities.
What can you expect?
BSc (Hons) Fashion Management students will develop skills to manipulate data and to model a range of business problems, functions and phenomena. Students undertake industry-led projects and meet fashion experts who will share their ideas and opinions with you. Recently we have welcomed in representatives from Net a Porter, Tiffany and Harvey Nichols.
This course is accredited by the Chartered Management Institute (CMI). This will give you the opportunity to graduate with a CMI Diploma level 5 in addition to your degree.
You will have the option of doing a placement year between the second and final year of the course. Successful completion of this placement year will give you an additional qualification. You will also have the opportunity to make contacts and build relationships within the industry. Students from this course have taken up placements with Debenhams, ASOS, River Island and Jimmy Choo. Throughout the course we support you with career planning sessions enabling you to make contacts and build relationships within the industry before you graduate.
Over the course of the degree students have the opportunity to enter external prizes and competitions. You will have the opportunity to attend relevant conferences and to visit a European fashion city on an optional trip in the first year.
London College of Fashion (LCF) undergraduate courses develop your personal and professional skills. On this course you will develop skills in your discipline until you are an independent creative thinker. Your skills will make you capable of making an effective contribution to this sector of the fashion industry. We embed Personal and Professional Development (PPD) skills in all units on every course. Speaker programmes with contributions from alumni and members of industry are a part of many courses. We encourage graduates who wish to continue their education at postgraduate level to progress to suitable courses within the College, the University or elsewhere.
The course is based at High Holborn near the central London areas of Lincoln's Inn Fields and Clerkenwell. Holborn is just a short walk away from The British Museum, Somerset House and the historic Leather Lane market. As well as many of LCF's Fashion business courses, High Holborn is home to UAL's Students' Union and a show room space hosting student led exhibitions.
About London College of Fashion
London College of Fashion, UAL, has been nurturing creative talent for over a century, offering courses in all things fashion. Through inspirational teaching we nurture the next generation of creative leaders and thinkers who work in responsible, analytical and ingenious ways. Our strategic commercial partnerships support local and global enterprise. Students benefit from our connections with industry and so does industry. Through teaching, specialist research, and collaborative work, we empower our students to think differently, using fashion to examine the past, build a sustainable future, and improve the way we live.
How to apply
Application deadlines
If your application is completed by the following date, it's guaranteed to be considered:
15 January*If you apply after this deadline, universities or colleges don't have to consider your application if they've filled their spaces, so the sooner you apply, the better!
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|Course code
|Institution code
|Campus name
|London College of Fashion
|Campus code
This course may be available at alternative locations, please check if other course options are available
Points of entry
The following entry points are available for this course:
- Year 1
Entry requirements
Qualification requirements
|Further information
|UCAS Tariff
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|Year 1
|Year 1
|Year 1
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272 High Holborn
Course contact detailsVisit our course page
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Please select a course option to view the information for the course


text: Afghans have been praised for defying Taliban threats and turning out in their millions to vote in crucial elections, which the US called a "great achievement" while pledging "continued international support". More than 50 percent of registered voters - about seven million people - were estimated to have turned out on Saturday for local and presidential elections, which will usher the country's first democratic transfer of power. "It is a proud day for this proud nation. We have proven that we are people of the ballot, not of the bullets," said Ashraf Ghani Ahmadzai, a presidential candidate. "This is a day of celebration." The outgoing Afghan president, Hamid Karzai, said: "Today's election and massive participation of the people have taken Afghanistan a few steps forward to peace, stability and development." Barack Obama, the US president, congratulated the Afghan people and said the event was a milestone as the Afghan people take full responsibility for their country. Unprecedented elections Preliminary results for the presidential poll are not due until April 24 and a run-off election is scheduled for May if there is no outright winner. There is no clear favourite but front-runners include Zalmai Rassoul, a former foreign minister; Abdullah Abdullah, the runner-up to Karzai in the 2009 election; and Ghani, a former World Bank academic. Whoever succeeds Karzai must lead the fight against the Taliban without the help of US-led troops, who are due to pull out by the end of 2014, and also strengthen an economy that currently relies on aid money. The Taliban had urged their fighters to target polling staff, voters and security forces, but there were no major attacks reported during the day. Thousands lined up outside polling centres in Kabul, which has been hit by a series of deadly attacks during the election campaign. "I'm not afraid of Taliban threats, we will die one day anyway. I want my vote to be a slap in the face of the Taliban," housewife Laila Neyazi, 48, told the AFP news agency. One blast in Logar province, south of Kabul, killed one person and wounded two, according to Mohammad Agha district chief Abdul Hameed Hamid. Omar Daudzai, the interior minister, said said four civilians, nine police and seven soldiers had been killed in violence on election day, and added that many attacks had been foiled, without giving further details. Attacks or fear of violence had forced more than 200 of a total 6,423 voting centres to remain closed. SOURCE: Agencies Politics Asia US & Canada Afghanistan United States


text:
Taylor Wimpey is staging a local exhibition of its plan to create a major new housing development in South Queensferry's South Scotstoun area.
The firm aims to submit a detailed planning bid for a scheme housing up to 470 homes and apartments on land beside the A90.
If given the go-ahead the project on "this attractive greenfield site" will be under construction early next year.
The exhibition event – to inform residents of "updated proposals" – is at the community centre behind Queensferry Primary School on Tuesday, November 15 from 4pm till 8pm.
Taylor Wimpey says: "Under our proposals, we would provide a mix of one, two and three bedroom apartments with off-street parking and landscaped gardens.
"A proportion of the new properties would be made available as affordable homes for local people in housing need.
"We also intend to provide a mixture of detached, semi-detached and terraced homes on our development."
South Queensferry Community Council has warned any residents who may decide to oppose the scheme that any comments or representations made to Taylor Wimpey at the event wouldn't count as representations against the plans.
Its spokesperson said: "Formal responses need to be lodged separately after the application has been submitted to Edinburgh Council."


text:
As a registered business in Ireland and Morocco, Taghazout Travel was formed in 2010. It consists
of a small international team of like minded individuals who have one thing in common…our
desire to share the beauty of Morocco, and in particular Taghazout, with others. Our aim is to plug
the gap of a much needed service for anyone looking to visit this magical and unspoilt area. Past
experiences had shown that anyone seeking to come to Taghazout were forced to make their own
travel arrangements, which at best were difficult, and at worst were nigh on impossible. In essence,
we act as a point of contact either online prior to travel, or in Taghazout itself. Whatever your
requirements, we can make it happen.
Making your holiday perfect is our speciality
We work closely with a variety of businesses in the area to give you everything you need to make
your holiday special. If you fancy surfing lessons and need to hire the kit, then no problem. If
you need transportation to and from the airport, then again we can arrange it. If you are in need of
accommodation or would simply like to explore more of this magnificent area, then our team can
make your dreams come true. Whether it's a romantic dinner for two or a horse riding trek into the
mountains, Taghazout Travel can organise as much or as little as you want.
Speaking of the team…
Collectively, our knowledgeable team can speak multiple languages, in particular English, French,
Dutch, Spanish, Arabic and Berber. This means that we have no problem in conversing with clients
from many countries around the world. In fact, we pride ourselves in delivering a quality, multi
national service, that is friendly and hassle free.
Taghazout Travel are a travel agency, travel service provider and travel website all rolled into one,
doing what we do best ….. organising everything you need to make your overseas trip a memorable
If you are curious about travelling to Morocco, in particularly the Taghazout area, then feel free to
contact us. Our knowledgeable team is here to help.


text:
It's funny looking back that I mentioned in this Q&A video that Tom leaving his job and us both working together was part of the bigger picture. I didn't really anticipate that happening for another 5 years so when we made the decision only a few months later it was amazing.
We'll be coming up to a year this August and I think I was finally sit down with Tom and run through any questions anyone might have about our decision (I have been promising to do this for over 6 months now whoops). It's not lost on us that all this was possible because of the support of you guys so for that I will always be so grateful!
Anyway I wanted to jump on a do a bit of an update (it feels like forever since I've done that, I blame the heat!)
I took a few selfies and wondered for the ten millionth time 'fringe or no fringe'
I met up with some lovely pals included this beaut Molly in the very instagramable Albatros Cafe in south Bristol.
We ate watermelon and my knees looked like angry little babies ;)
Me and Mabli played lots of silly games. I absolutely love this age, she is such a little character! I think when Wilf was two and a half I started to feel like I wanted another child for the first time because it was just such a wonderful stage of our lives. We are defo done with having any more kids so I need to just soak up these lovely ages!
We also ended up dressing up as three lions haha, actually it was four lions..and we looked more like tigers. It made NO SENSE! but was fun all the same
Tom turns 36 in a week and we're not planning anything big (actually we never plan anything big so I don't know why I said that) especially with moving soon. I am going to take the kids out to pick up little gifts they picked out and then I think we're just going to go out and eat pasta.
We've been half enjoying the sun half melting in the heat. I know it wont last much longer so I'm trying my best not to complain but the evenings are the worst when it's still 29 degrees in their room (even with two fans!).
I think thats it for the past couple of weeks, I'm trying to update my shop my instagram page too so check over there.
Are you coping in the heat? whats been your favourite thing over the past fortnight? I'd love to know!
I think thats it for the past couple of weeks, I'm trying to update my shop my instagram page too so check over there.
Are you coping in the heat? whats been your favourite thing over the past fortnight? I'd love to know!