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In this section we will include information about social events and walks that are organised or attended by Admin coming up and eventually some photos or further information about those events if there's anything interesting!
Social meet ups can be with or without dogs as not everyone's dogs like to mix with other dogs and not everyone has friends who like dogs! In fact, some members don't even have a dog, however, everyone is welcome. It's always great to catch up with people from time to time who we otherwise only know as a profile picture in the group! Many a friendship has been started or rekindled within the group. 🐶
Scroll down for information about past events including those with or without dogs.
GDPR Privacy Notice
Privacy Notice The information we collect from you includes: 🐶 Full Name 🐶 Email Address 🐶 We also collect pictures…Read more.
Carole Beadle on Competitive Obedience
Written by Carol Beadle (One 2 One Dog Training Jersey) 8 April 2018 I became involved in competitive obedience early…Read more.
French Bulldog Saviours – Jersey
Article By: Sofia Ribeiro Date of Article: 11 February 2018 Hello dear JDF followers The lovely Carla Channing asked me…Read more.
Save & Care Dog Rescue – Romania
Article by: Esta Williams Date of Article: 26 January 2017 Esta Williams rescued Parker Junior ('Parkie') from Save & Care…Read more.
Live Chat with Jo Laurens (DogWorks) – 23rd January
This Tuesday 23 January 2018 between 7pm and 8pm, Jo Lauren's was online in the Jersey Dog Forum Facebook group…Read more.
Jersey Dog Training Association Class Dates 2018
The Jersey Dog Training Association has released its 2018 class start dates. All breeds are welcome. THURSDAY 4 JANUARY 2018.…Read more.
Dunes walk with Islana McLaughlin Bell (IMDT Trainer) and Dexter, Dexter and Jess' Birthdays!
Good evening Jersey Dog Forum This Sunday we will be meeting for a walk at the dunes at 9.30am. We…Read more.
Loss of a Pet
Frequently Asked Questions The JSPCA has kindly provided us with answers to some questions which none of us want to ask…Read more.
Jersey Dog Handlers Association Nominated for the Pawscars!
The Jersey Dog Handlers Association ("JDHA") has been shortlisted down to the final 3 out of the whole of the UK…Read more.
Can you help Ariel ?
Article by: Barbara Keywood (JSPCA) Date: 28 December 2017 Ariel is an extremely pretty and loving 3 year old Doberman…Read more.
JDF Boxing Day Walk – 9:30am @ The Dunes
It's nice to blow those Christmas Day cobwebs away with a fresh walk first thing boxing day morning. All shapes…Read more.
Finding a qualified Physiotherapist / Hydrotherapist
Written by: Sarah Lewis (BSc CVPhys MIRVAP CERP CertEdVPT) (Physiotherapist, New Era Veterinary Hospital) 15 December 2017 Dog fitness…Read more.
IMDT Partnership Award
Jersey Dog Forum Group members "Parker" and "Indie" have recently passed their IMDT Partnership Award and have written a little…Read more.
Company of Dogs Table Top Sale – 16th December 2017 – 9am to 2pm
Company of Dogs have a table top sale on Saturday 16th December 2017 at 9am to 2pm at St Andrews…Read more.
Could your dog have their sight restored by Animal Health Trust?
Ziggy needs his cataracts operated on and it's not cost effective for the surgeon to come over for just one…Read more.
Jersey Big Dog Club at Fort Regent
The Jersey Big Dog Club is up at Fort Regent today & tomorrow from 2-30 at Iceskate Jersey for a…Read more.
Wyatt's Birthday Walk Photos – December 2017
Hello, I just wanted to add some photos from Mr Wyatt's Birthday walk held on Sunday at the dunes, we,…Read more.
"Delta" (Jersey Rescue Dogs)
"Delta" Delta is looking for a home. Rescue - Jersey Rescue Dogs Age - 18 months Kids - 5 +…Read more.
RIP 'Polar Bear'
This morning we have received the very sad news that Polar Bear's body was found on the cliff path between…Read more.
"Polar Bear" Poster
Esta Williams who is helping Polar Bear's family has asked if we could distribute the attached poster (below) around the…Read more.
"Polar Bear" has been missing from Plemont since Thursday
UPDATED: Sadly there is still no real update or news on Polar Bear. Some lovely people have been helping the…Read more.
Teddy (Kiki Beck)
Teddy was found on the streets of Bosnia. He is currently in kennels in the UK but his rescuers are…Read more.
A Happy Stress Free Christmas for your Dogs
A happy stress free Christmas for your Dog Written By Rosie Barclay A happy stress free Christmas for your dog.…Read more.
Wyatt's birthday walk – Sunday 3rd December – 10am Sand Dunes
🐶 Wyatt's birthday walk 🐶 Sunday 3rd December – 10am Sand Dunes Many of you have followed Wyatt's story in…Read more.
Jersey Dog Forum Christmas Meal – 8 December 2017
It had to be done I suppose... JDF Christmas meal at the Farmhouse. 7pm 8th December 2017🎄 All welcome but…Read more.
Live Facebook Chat with Behaviourist Rosie Barclay
On Thursday 23rd November 2017 between 7pm and 8pm, there was an "Ask the Behaviourist" chat in the Jersey Dog…Read more.
JEP post – Police to investigate financial matters at JSPCA
The JEP has issued the following article: https://jerseyeveningpost.com/news/2017/11/22/police-to-investigate-financial-matters-at-jspca/TestingRead more.
Rescue Dogs Looking for a Home
We love a bit of rescue… so we have created a page where you will find links to various dogs…Read more.
Eddie (Company of Dogs)
Eddie is looking for a home: Rescue - Company of Dogs Age - About a year old Kids - No…Read more.
German Shepherd Walk 18th November
Liam here, I've snuck into the website, dangerous or clever? Unfortunately Carla knows where I live so I better be…Read more.


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It is not always necessary to hire a personal injury lawyer, but if you are injured in an accident and you are not at fault, hiring someone to help can make a world of difference. Fighting your case the right way can help you to get the medical treatment you need, receive funds for lost work and even receive personal compensation for your pain and suffering. This article will explore many of the factors involved in a personal injury claim. Continue reading to learn more.
When choosing a personal injury attorney, ensure that you are choosing a law office which has an entire department dedicated to personal injury. Meanwhile, it is common for law offices to pursue several different types of cases, it is important to choose an attorney who's well versed in your niche.
When fighting a personal injury case, a lawyer will have many curve balls thrown at him. That means that only a lawyer with an extensive history in the field of personal injury law will be able to be successful at trial. Look for a lawyer with lots of experience behind him.
In this particular field of law, experience is definitely worth the extra money it may cost. A personal injury lawyer that has been practicing for some time, and who has many cases under their belt, is certainly going to be well versed in how to obtain the maximum settlement sum. You will get what you pay for.
If the lawyer you are talking to normally works with insurance companies, you probably want to pass on their services. Instead, you want someone who has experience helping the injured person. These personal injury attorneys should have the background that you need to successfully win your case, and you will probably feel more comfortable with them as a result.
Look for a lawyer willing to work on a contingency basis. This means that your lawyer will not get paid unless you receive a settlement. Not only will this mean your lawyer has a vested interest in getting you a good settlement, it also means you won't be left with a large legal fee should your case be dismissed.
If you have to purchase and prescriptions or medical equipment as a result of an injury, make sure that you get the receipts and turn them in to your attorney. He will submit them as evidence and it may help you receive a bigger settlement than you had originally anticipated.
A personal injury case, when brought to court, can help an individual to receive compensation for lost time, wages and the pain and suffering involved in an accident where the individual is not at fault. If you find yourself in such a situation, take the time to explore all of your options and make the right decisions regarding who you hire and how you represent your case. Doing so can increase your changes of winning and help you to recoup the things you need to get back to a more normal life once again.
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WINTER HAVEN - Aviation writer, pilot and flight instructor Jamie Beckett entered the race for Winter Haven city commissioner by way of the Winter Haven Municipal Airport - or his outspoken criticism of the facility's expansion.
"I was asked to run after I wrote a column critical of the airport expansion," said Beckett, who was a regular newspaper columnist for the News Chief until he declared his intent to run for a commission seat.
Beckett is challenging incumbent Yvonne Brooks, the city's current mayor, for Seat 4 in the Sept. 8 city election.
The new airport terminal off U.S. Highway 92 is in the wrong place, Beckett said, and this presents safety issues.
"It should have been designed differently," he said, adding that this could have been prevented if city commissioners had listened to pilots who use the airport regularly.
And this, according to Beckett, is indicative of the change that needs to happen on the City Commission.
"There is a lack of public participation in the government," he said, citing the airport expansion as an example. "People are frustrated. They feel like City Hall doesn't listen to them."
This wasn't always the case, Beckett said.
"Eight years ago, there was a terrific amount of communication. You knew why (the commissioners) voted the way they did," he said. "In months of going to meetings now, I can't tell you why the commission voted the way it did.
"When the public has no input from commissioners, they have no basis to form an opinion," he explained.
Beckett said that if elected, it will be his responsibility to improve public input on city matters.
"Public access is not an option," he said, making the point that the public's access to government and government officials should be the standard.
Despite his aviation history and comments on the airport terminal, Beckett, 50, said he isn't "the aviation candidate."
Born in Phoenix, Ariz., and raised in Glastonbury, Conn., Beckett moved to Winter Haven with his wife, Karen Flynn, in 1991 to work as a flight instructor. The father of three - Dylan, 25; Nikki, 15; and Madison, 10 - soon became interested in city government and served five years on the Winter Haven Code Enforcement Board. Eight years ago, he ran unsuccessfully against Mike Easterling for the City Commission.
City commissioners serve four-year terms and are paid $11,590 a year.
Beckett said he wants to see the baseball facilities at the Chain O' Lakes Complex used more and the city take a more active role in marketing the airport, ballparks and canals.
He also thinks priority spending is an issue - and his priorities relate to public safety.
"Police and fire are priority needs," he said. "People need to know they can call and get help without thinking, 'Will I be billed?' "
Beckett is concerned about what he views as mounting city debt and asks why money was spent on such purchases as the Hill Building, which was located on Third Street until it was recently demolished. Passed by the City Commission with no discussion as a consent agenda item, he questions why the $1.2 million purchase of what some consider a historical landmark was approved without public discussion.
"The consent agenda is designed for the renewal of current service" and not major purchases, Beckett said.
Beckett said the money spent on the Hill Building could have been used to renovate the training center at the airport so it could be used for fire and police training.
"This would be cheaper than outsourcing training," said Beckett, who thinks this also would attract a larger number of police and fire department employees and enhance their training.
And while Beckett said he understands the need for conserving natural resources, he thinks the city may be overdoing it a bit.
He applauds the new fire station currently under construction, which he believes is needed, but he is not happy about the $5.2 million price tag on the facility designed with "green," or environmentally friendly, standards in mind.
"While I'm not happy about the cost, we do need a better fire station," he said. "We need a place for our firefighters and police officers to be safe during a really big storm.
"I think the city of Winter Haven has taken the green thing to the point where people don't understand it. It's become a marketing tool," Beckett said.
He uses the recent purchase of BMW motorcycles for the police department as an example.
"The gas mileage (on the BMWs versus the previous Harley-Davidson police motorcycles) is not better and they are more expensive, yet they billed this as part of their green initiative. There's nothing green about it," he said.
Beckett also wonders about the contradictory nature of some city policies. For instance, he said, one department is working to increase the amount of natural surfaces to retain water while another requires impervious surfaces for parking lots.
"I think we can have a valid green initiative in this city," Beckett said. "If you're going to embrace a green initiative, you have to embrace the entire thing."
Beckett said he most differs from Brooks in how he views the city's most important strength.
"I disagree with her on a number of issues, but I have a lot of respect for her," he said. "Yvonne thinks the greatest city strength is the staff. I think it is the people who live there."
He describes the current City Commission as "the Rubber Stamp Patrol" that votes the way the city manager tells them.
"I think the City Commission has forgotten the city manager works for them," he said. "The commissioners' job is to represent the people."
Janeen Kochan, who has known Beckett for about five years, said he would be a refreshing change to the commission.
"He listens to citizens' concerns. He does the research to find the best possible solutions to those concerns and he acts on them," Kochan said.
Kochan, a pilot and designated pilot examiner, described Beckett as level headed, down to earth, fiscally responsible and honest.
Chris Moran, who is retired from the U.S. Air Force, said Beckett has a vision and insight into change.
"He is willing to buck the system. He won't just go along with the boys," Moran said.
Jamie Beckett: Get public involved in government
WINTER HAVEN - Aviation writer, pilot and flight instructor Jamie Beckett entered the race for Winter Haven city commissioner by way of the Winter Haven Municipal Airport - or his outspoken criticism of the facility's expansion.


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BRIDGEWATER, Mass. -- Sophomore Shaelyn Carreiro (Fall River, Mass.) notched a double-double effort of 20 points and 11 rebounds to lead UMass Dartmouth to a 66-62 non-league college women's basketball victory over Bridgewater State Tuesday evening at the Tinsley Center.
After jumping out to a 17-13 first quarter edge, the visiting Corsairs (4-1) received seven points from sophomore Jordan Moretti (Johnston, R.I.) as part of an 18-point outburst in the second stanza to extend that margin to 14 (35-21) at the break. The host Bears (0-2) scored seven of the first eight points after intermission to claw back to within eight (36-28) on sophomore Maggie Hester's (Braintree, Mass.) lay-in, but senior Ashley Brown (New Bedford, Mass.) sparked a 13-3 UMass Dartmouth run in a six-plus minute stretch that pushed the visitors' lead to 49-31 late in the third quarter.
Bridgewater State responded with an 11-1 run of its own to close out the frame and pull back to within eight (50-42) heading into the final 10 minutes of action, but the Corsairs regained a double-digit edge early in the stanza and maintained a six-point advantage until the closing minutes of action. Two fast break lay-up conversions by Carreiro (coupled with a dribble drive by Moretti) gave UMass Dartmouth a seven-point lead (66-59) with 39 seconds remaining, and the Bears used a three-pointer from sophomore Hannah Dziadyk (Wakefield, Mass.) with 14 seconds left to account for the final margin but get no closer on their final possession.
Brown added a double-double performance of 17 points, 11 rebounds, eight assists and three steals in the win for the Corsairs, who received 11 points and four assists from Moretti. Junior Lauren Empey (Middleboro, Mass.) chipped in with seven points and eight boards for UMass Dartmouth, which received a combined 10 points from sophomore Jessica Carroll (Deep River, Conn.) and junior Meagan Donovan (Abington, Mass.). The visitors shot 41 percent (23-55) from the field and won the game at the foul line, as the Corsairs outscored the hosts by a 15-1 margin at the charity stripe.
Sophomore Olivia Dziadyk (Wakefield, Mass.) netted 18 points, five assists and four rebounds in the setback for Bridgewater State, which received 12 points from Hannah Dziadyk as well as 10 points, nine boards and three assists from Hester. Senior Nicole Bostick (East Taunton, Mass.) chipped in with nine points, four rebounds and three assists for the Bears, who received seven caroms from freshman Kylee Piche (Bridgewater, Mass.). The hosts shot 34 percent (26-76) and connected for nine three-pointers, as each team grabbed 44 rebounds on the evening.
UMass Dartmouth travels to Gorham, Maine on Saturday afternoon to take on Southern Maine in its Little East Conference opener that tips-off at 1:00 pm, while Bridgewater State entertains Salve Regina in opening round play of the 2019 Bridgewater State Cave Tip-Off Tournament on Friday evening at the Tinsley Center that tips-off at 5:30 pm.


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Beds: 3 Baths: 1 Car Park: 3
Property Description
This property is currently not available.
Conveniently located close to the Catholic college and Kingaroy CBD.
This house features..
3 Bedrooms main with bay window, High ceilings and vj walls, Large lounge & dinning room, Bright and airy sun room, Spacious Kitchen, Modern bathroom, Separate office, Ducted evaporative cooler
1012 sqm block, Equipped bore, 6×6 colour bond shed, Single car accommodation and workshop under house, manicured lawns and gardens
Please call Ray White Kingaroy on 41604700 to arrange an inspection.


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Right on!
Also found in: Wikipedia.
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right on
1. An indication of support, enthusiasm, agreement, or encouragement. A: "I got the job!" B: "Hey, right on, man! That's great news." A: "I was thinking we could go for burritos for lunch." B: "Right on, that sounds good to me."
2. Exactly right; perfectly accurate. Mr. Lynch's analysis of the company is right on, if you ask me. I suggest we follow whatever advice he offers.
Right on!
Sl. Exactly!; That is exactly right! After the speaker finished, many people in the audience shouted, "Right on!" One member of the crowd called out, "Right on!"
Right on!
exclam. Exactly!; That is exactly right! After the speaker finished, many people in the audience shouted, "Right on!"
right on!
Keep going; you're on the right track/doing well. This term dates from the early twentieth century, and there is some dispute over its origin. Several sources trace it to the 1920s in African-American speech; another holds it is an American version of the British Bang on! used by airmen during World War II, or possibly a shortening of Right on target. Still another holds it is a shortening of Right on cue, a reference to uttering the correct lines in a play. In any event, it became widely used in the 1950s and 1960s. Kate Millett had it in Flying (1974): "Right on, Vita, so you must have waged your woman's war for years."


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Welcome to the website of X-PLOR e.V. - an organization for students, researches and others who are interested in production, logistics, operations research, and business analytics!
On the following pages, we would like to inform you about our organization, our memebers and our activities.
X-PLOR wishes all memebrs a succesful summer semester 2019. The current program can be found here: LINK


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Virgin Radio has dropped DJ Daryl Denham and Jezza, the host of its daily Confessions show. Jezza - Jeremy Kyle - who has presented the daily Virgin Confessions phone-in show from 22.00 to 01.00 for two years, has agreed to step down. But Denham, who was shifted into the weekend ...
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Monday, August 10 – Thursday, August 13
9am-3pm (Drop off 8:30-9am, pickup promptly at 3pm)
Blackwater Falls State Park, Pendleton Point Picnic Shelter
Ages: 5-8
Cost: TBD
Go on adventures in wild places and play games outside with your friends!
APEX Wild Day Camp will take participants on a tour of the wild places surrounding our community. We will get to know these places through adventures, curiosity, and lots of games! We will romp in the woods, play in the rain, and look for wild plants and animals while getting to know each other better.
What to bring:
- Dress for the weather of the day
- Small Backpack
- Water Bottle
- Please make sure to pack a lunch each day for your child. Morning and afternoon snacks will be provided. If your child has dietary restrictions, please pack two additional snacks daily and share dietary information when registering your child. Please note that we are not an allergen-free camp.
- Rain Jacket
- Extra set of clothes and shoes
- Warm jacket/hoody
- Sun hat/baseball cap
- Swim suit and towel
- Sunscreen (we will have some, but if there is a kind your child prefers)
- Water shoes or sneakers you don't mind getting wet for creek and lake days
- Prescription Medications (i.e. inhalers, epinephrine; we will collect and distribute each day)
Where to go:
Directions to Pendleton Point Picnic Shelter in Blackwater Falls State Park
From "downtown" Davis, head west (towards Thomas) on William Ave for .5 miles. At the BFS/Marathon Gas Station, turn Left onto Blackwater Falls Rd for 1.2 miles. Turn Right at the 4-way intersection (follow signs towards Campground, Pendleton Lake, Nature Center) for .7 miles. You will see a small sign on your right for Pendleton Point Picnic Shelter. Turn Right into the Pendleton Point parking lot and walk your child across the street. We will be waiting excitedly for you at the shelter!


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We are a viable and enterprising company, proud of our heritage, we strongly believe in the power of our brand and we are totally committed to our brand of products. Our vision is to touch the lives of our millions of consumers everyday through helping them feel good, look good, keep their homes and environment fresh and get more out of life with brands and services that are good for them.
We intend on executing this vision by operating within a clear set of rules.
QUALITY: our customers deserve quality products. We must fulfill our promise to provide products of consistently high quality.
VALUES: We need to demonstrate the highest standards of corporate governance and corporate and social responsibility throughout the Group. We have clear obligations to consumers, suppliers, our employees and to the communities in which we operate. These obligations are respect, integrity and openness.
PROFITABILITY: As our business grows, so do our responsibilities. We develop new ways of doing business with the aim of doubling the size of our company while reducing our environmental impact.


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|HomeIndoorVacuum CleanersMieleComplete C3 Series41GFE037USA
+ Product Information
Brand: MIELE
Item Number: 41GFE037USA
Model: 41GFE037USA
Series: Complete C3 canister vacuum
+ Appearance
Color: Obsidian Black
+ Technical Details
Energy Star Rated: No
Miele 41gfe037usa
This lightweight capsule vacuum from Miele features a 1,200-watt Vortex Motor with 6 suction settings, making it equipped to clean all floor types, from hardwood to deep carpeting. A HEPA AirClean filter eliminates 99.95% of all lung-damaging particles, and the airtight vacuum chamber features a spring-loaded, self-locking collar. In other words, once debris is vacuumed up with a C3, it's gone for good. On top of outstanding cleaning performance, the vacuum offers a generous 36-foot cleaning radius for maximum convenience. This selection from Miele brings you powerful cleaning potential for a spotless and more sanitary home.
CA Residents: Prop 65 WARNING


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"The development of the next generation of Electric Machines can only meet the performance, efficiency and levels of sustainability required in response to market demand by leveraging the full potential of state-of-the-art digital tools and methods. A key success factor in achieving these goals is by using holistic development approaches based on Simulation-Driven Design and multi-physics optimization, something that we are excited to be exploring with our Simulation-driven Innovation partner Altair across a range of special talks and case studies at CWIEME Berlin this year"
Chloe Theobald, Head of content CWIEME
Coupled Electro-Magnetic and Acoustic Simulation of an In-wheel Electric Motor
At Elaphe, the engineers have been facing the NVH challenges from the very beginning. The topology of this electric motor, which on the one hand enables the team to use the otherwise empty space inside the wheel, can on the other hand, result in some new and unexplored NVH challenges. The experience over the years has proven that NVH is a bottleneck in the design cycle of Elaphe's motors and this was the main motivation for a more automated and more user-friendly NVH simulation workflow. Within the NVH, noise radiation was the area Elaphe was most interested in.
Improving Electric Vehicle Range with Advanced Losses Computation
Introducing electric traction in automotive brings new challenges for the design of electric machines. Nowadays designers have to consider increasing constraints like efficiency, temperature, weight, compactness, cost but also stricter regulations, while reducing time to market. Fortunatly, Altair proposes disruptive methodologies to make relevant choices in the early stage of the design, based on numerical simulation and optimization techniques.
This webinar covers how an electric motor design's performance is evaluated and maximized considering its global efficiency along the whole driving cycle.
E-Motor Weight and Cost Reduction with Efficient Design and Optimization
This webinar will introduce Altair's e-motor design and optimization solutions in a step by step process. We will discuss pre-design, magnetic computation and thermal analysis and show how optimization methods can help to optimize weight and cost at each step of the process (especially the weight of magnets) . We'll demonstrate how Design Of Experiments (DOEs) allows designers to run different types of optimization very quickly, which enables informed decisions at different stage of the design cycle.


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Journal of Chromatography B (v.879, #15-16)
Editorial Board (i).
Simultaneous quantification of tracheloside and trachelogenin in rat plasma using liquid chromatography/tandem mass spectrometry by Li Li; Fanhua Meng; Jifen Guo; Lu Sun; Nengjiang Yu; Yimin Zhao (1033-1037).
We developed and validated a quantitative method for simultaneously determining the concentrations of tracheloside and trachelogenin in rat plasma. Plasma samples were prepared by liquid–liquid extraction with ethyl acetate. Isocratic chromatographic separation was performed on a reversed-phase Diamonsil C18 column (4.6 × 200 mm, 5 μm). The mobile phase consisted of methanol and 10 mM aqueous ammonium formate (80:20, v/v). Analyte detection was achieved by positive electrospray ionization (ESI) tandem mass spectrometry. Calibration was performed by internal standardization with glipizide, and regression curves ranging from 0.625 to 625 ng/mL were constructed for both the analytes. The intra- and inter-day precision values were below 8%, and accuracy ranged from −5.33% to 2.53% in all quality control samples. In this study, the validated method was successfully applied to determine the pharmacokinetic profile of tracheloside and trachelogenin in rat plasma after oral and intravenous administration of trachelospermi total lignans.
Keywords: Lignan; Tracheloside; Trachelogenin; Tandem mass spectrometry; Quantification; Pharmacokinetics;
Simultaneous determination of eight β-lactam antibiotics in human serum by liquid chromatography–tandem mass spectrometry by Tomofumi Ohmori; Akio Suzuki; Takashi Niwa; Hiroaki Ushikoshi; Kunihiro Shirai; Shozo Yoshida; Shinji Ogura; Yoshinori Itoh (1038-1042).
A liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed for the simultaneous determination of eight β-lactam antibiotics, including ampicillin, cefazolin, cefepime, cefmetazole, cefotaxime, doripenem, meropenem, and piperacillin, in human serum. Sample specimens were subjected to solid phase extraction (SPE) using Waters Oasis® HLB cartridges (30 mg). Chromatographic separation was performed with a high-resolution octadecyl silica column compatible with hydrophilic compounds, using a gradient of 10 mM aqueous ammonium formate containing 0.1% formic acid-methanol. Antibiotics were detected by a triple quadrupole mass spectrometer (MS/MS) with electrospray ionization and quantified by the multiple reaction monitoring mode. A total run time of 13 min was applied. Linearity in the calibration was obtained over a range of 0.1–50 μg/mL of the β-lactam antibiotics, except for doripenem. The lower limit of quantification was 0.005–0.5 μg/mL, using 50 μL serum. The recovery rate exceeded 80.2% for these analytes, except for doripenem (49.1%) and meropenem (62.3%). The present method is applicable to routine therapeutic monitoring of β-lactam antibiotics in clinical practice.
Keywords: β-lactam antibiotics; Liquid chromatography; Tandem mass spectrometry; Solid phase extraction;
A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography by Jian-Bo Qu; Yong-Dong Huang; Guang-Lun Jing; Jian-Guo Liu; Wei-Qing Zhou; Hu Zhu; Jian-Ren Lu (1043-1048).
Agarose coated gigaporous polystyrene microspheres were evaluated as a novel matrix for immobilized-metal affinity chromatography (IMAC). With four steps, nickel ions were successfully immobilized on the microspheres. The gigaporous structure and chromatographic properties of IMAC medium were characterized. A column packed with the matrix showed low column backpressure and high column efficiency at high flow velocity. Furthermore, this matrix was used for purifying superoxide dismutase (SOD), which was expressed in Escherichia coli (E. coli) in submerged fermentation, on an Äkta purifier 100 system under different flow velocities. The purity of the SOD from this one-step purification was 79% and the recovery yield was about 89.6% under the superficial flow velocity of 3251 cm/h. In conclusion, all the results suggested that the gigaporous matrix has considerable advantages for high-speed immobilized-metal affinity chromatography.
Keywords: Gigaporous; Polystyrene microspheres; Column efficiency; Immobilized-metal affinity chromatography; Superoxide dismutase;
Metabolism studies of the Kratom alkaloids mitraciliatine and isopaynantheine, diastereomers of the main alkaloids mitragynine and paynantheine, in rat and human urine using liquid chromatography–linear ion trap-mass spectrometry by Anika A. Philipp; Dirk K. Wissenbach; Armin A. Weber; Josef Zapp; Hans H. Maurer (1049-1055).
Mitragyna speciosa (Kratom in Thai), native in Southeast Asia, is increasingly misused as a herbal drug of abuse. During metabolism studies on the Kratom alkaloids mitragynine, its diastereomers speciogynine and speciociliatine as well as paynantheine in rats and humans, further isomeric compounds were detected in Kratom users' urine. The question arose whether these compounds were formed from the low abundant, isomeric alkaloids mitraciliatine (MC) and isopaynantheine (ISO-PAY). Therefore, the aim of the presented study was to identify using liquid chromatography–linear ion trap-mass spectrometry their phase I and II metabolites in rat urine after administration of pure MC or ISO-PAY, to confirm their formation in humans, and finally to confirm whether the above-mentioned isomeric compounds in human urine represent MC and ISO-PAY and/or their metabolites. The metabolic pathways of both alkaloids in rats were found to be comparable to those of their corresponding diastereomers. In the human urines tested, not all metabolites found in rats could be detected because of the much lower amounts of MC and ISO-PAY in Kratom. However, all the above-mentioned so far unknown isomeric compounds could be identified in the human urine samples as MC, ISO-PAY and/or their metabolites. The used LC separation was also suitable for the differentiation of all other Kratom alkaloids and their metabolites in human urine.
Keywords: Kratom; Mitraciliatine; Isopaynantheine; Metabolism; LC–MS n ; Urine;
Novel multi-mode ultra performance liquid chromatography–tandem mass spectrometry assay for profiling enantiomeric hydroxywarfarins and warfarin in human plasma by Drew R. Jones; Gunnar Boysen; Grover P. Miller (1056-1062).
Coumadin (R/S-warfarin) is a commonly prescribed anticoagulant for over ∼20 million Americans. Although highly efficacious, positive clinical outcomes during warfarin therapy depend on maintaining a narrow therapeutic range for the drug. This goal is challenging due to large inter-individual variability in patient response, which has been attributed to diversity in drug metabolism. Warfarin is given as a racemic mixture and evidence suggest differences of R and S-warfarin in their therapeutic activities and metabolism. Previous investigation of warfarin metabolism has been hampered by the inability to quantify the individual enantiomers. To overcome this limitation a multi-mode LC–MS/MS method is reported. This strategy combines phenyl based reverse phase chromatography with chiral phase chromatography prior to quantitation by liquid chromatography tandem mass spectrometry. This approach was made possible through advances in UPLC technology producing narrow peaks suitable for transferring to a second column. The reported method separated individual R and S enantiomers of hydroxywarfarin and warfarin. All four possible isomers of 10-hydroxywarfarin were resolved to reveal unprecedented insights into the stereo-specific metabolism of warfarin. Characterization of the method demonstrated that it is robust and sensitive with inter-day coefficients of error between <7% and a detection limit of 2 nM in sample or 10 fmol on column for each analyte. Individual metabolites may be suitable surrogate biomarkers or predictive markers that predict warfarin dose, adverse interactions, or other important clinical outcomes during anticoagulant therapy. Consequently, the metabolite profiles obtained through this dual phase UPLC–MS/MS method are expected to increase our understanding of the role warfarin metabolism plays in patient response to therapy and yield new strategies to improve patient outcomes.
Keywords: Chiral; In-series; LC–MS/MS; Metabolic profiling; Warfarin;
Determination of moxifloxacin in dried blood spots using LC–MS/MS and the impact of the hematocrit and blood volume by D.H. Vu; R.A. Koster; J.W.C. Alffenaar; J.R.B.J. Brouwers; D.R.A. Uges (1063-1070).
Moxifloxacin (MFX) is a potential oral agent use in the treatment of multidrug-resistance tuberculosis (MDR-TB). Due to variability in pharmacokinetics and in vitro susceptibility of causative bacteria, therapeutic drug monitoring (TDM) of MFX is recommended. Conventional plasma sampling for TDM is facing logistical challenges, especially in limited resource areas, and dried blood spots (DBS) sampling may offer a chance to overcome this problem. The objective of this study was to develop a LC–MS/MS method for determination of MFX in dried blood spots (DBS) that is applicable for TDM. The influence of paper type, the hematocrit (Hct) and the blood volume per spot (V b) on the estimated blood volume in a disc (V est) was investigated. The extracts of 8 mm diameter discs punched out from DBS were analyzed using liquid chromatography tandem mass spectrometry (LC–MS/MS) with cyanoimipramin as internal standard. The method was validated with respect to selectivity, linearity, accuracy, precision, sensitivity, recovery and stability. The effect of Hct and V b on LC–MS/MS analytical result was also investigated. The relationship between MFX concentrations in venous and finger prick DBS and those in plasma was clinically explored. V est was highly influenced by Hct while the effect of V b appeared to be different among paper types. Calibration curves were linear in the range of 0.05–6.00 mg/L with inter-day and intra-day precisions and biases of less than 11.1%. The recovery was 84.5, 85.1 and 92.6% in response to blood concentration of 0.15, 2.50 and 5.00 mg/L, respectively. A matrix effect of less than 11.9% was observed. MFX in DBS was stable for at least 4 weeks at room condition (temperature of 25 °C and humidity of 50%). A large range of Hct value produced a significant analytical bias and it can be corrected with resulting DBS size. A good correlation between DBS and plasma concentrations was observed and comparable results between venous DBS and finger prick DBS was attained. This fully validated method is suitable for determination of MFX in dried blood spot and applicable for TDM.
Keywords: LC–MS/MS; Dried blood spot; Moxifloxacin; Tuberculosis; Pharmacokinetics; Hematocrit;
Group-selective molecularly imprinted polymer solid-phase extraction for the simultaneous determination of six sulfonamides in aquaculture products by Xizhi Shi; Yuan Meng; Jinghua Liu; Aili Sun; Dexiang Li; Chunxia Yao; Yin Lu; Jiong Chen (1071-1076).
Group-selective molecularly imprinted polymers (MIPs) made from sulfonamides (SAs) using functional monomer methacrylic acid (MAA) were synthesized. The derived molecularly imprinted solid-phase extraction (MISPE) cartridges were developed for the purification and enrichment of aquatic products. The optimum template molecule and the ratio of the functional monomer to the template for obtaining group selectivity to SAs were sulfadimethoxine (SDM) and 4:1, respectively. The MIPs were characterized by Brunauer–Emmett–Teller (BET), scatchard plot, and chromatography analysis, all of which demonstrate better chromatographic behavior and group-selectivity of MIPs for SAs compared with those of corresponding NIPs. The extraction conditions of MISPE for six SAs were optimized; the method precision and accuracy were satisfactory for the fish and shrimp samples at 0.05, 0.1, and 0.2 mg kg−1 spiked levels. Recoveries ranging from 85.5% to 106.1% (RSD, 1.2–7.0%, n = 3) were achieved. The limits of detection (S/N = 3) and quantitation (S/N = 10) in the shrimp and fish samples were achieved from 8.4 to 10.9 μg kg−1 and from 22.4 to 27.7 μg kg−1, respectively. Therefore, the obtained MIPs and MISPE can be employed for the enrichment and clean-up of SAs. This paper presents a new analytical method which enables the simultaneous determination and quantification of SAs in aquaculture products.
Keywords: Molecularly imprinted polymers; Sulfonamides (SAs); Group selectivity; Aquaculture products;
Identification of benzo[c]phenanthridine metabolites in human hepatocytes by liquid chromatography with electrospray ion-trap and quadrupole time-of-flight mass spectrometry by Pavel Kosina; Jan Vacek; Barbora Papoušková; Marie Stiborová; Jakub Stýskala; Petr Cankař; Eva Vrublová; Jitka Vostálová; Vilím Šimánek; Jitka Ulrichová (1077-1085).
The metabolism of the benzo[c]phenanthridine alkaloids was studied using human hepatocytes which are an excellent model system for biotransformation studies. For analysis of the alkaloids and their metabolites, an electrospray quadrupole ion-trap mass spectrometry (ESI ion-trap MS) connected to a reversed phase chromatographic system based on cyanopropyl modified silica was used. The optimized experimental protocol allowed simultaneous analysis of the alkaloids and their metabolites and enabled study of their uptake into and interconversion in human hepatocytes. The results show that formation of the dihydro metabolite which may be followed by specific O-demethylenation/O-demethylation processes, is probably the main route of biotransformation (detoxification) of the benzo[c]phenanthridines in human hepatocytes. The structure of the main O-demethyl metabolite (2-methoxy-12-methyl-12,13-dihydro-[1,3]dioxolo[4′,5′:4,5]benzo[1,2-c]phenanthridin-1-ol; 336.1 m/z,) was proposed by the multi-stage MS and quadrupole time-of-flight MS methods using chemically synthesized standard.
Keywords: Sanguinarine; Chelerythrine; Mass spectrometry; Biotransformation; Reduction and oxidation; Human hepatocytes;
Analysis of hydroxylated polybrominated diphenyl ethers in rat plasma by using ultra performance liquid chromatography–tandem mass spectrometry by Yongquan Lai; Xueguo Chen; Michael Hon-Wah Lam; Zongwei Cai (1086-1090).
Ultra performance liquid chromatography (UPLC) provides improved resolution, speed and sensitivity compared to conventional high performance liquid chromatography (HPLC). In this study, a robust UPLC–ESI–MS/MS method was developed for the rapid determination of nine hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in rat plasma. Under the optimized conditions, the OH-PBDE congeners were eluted within 7.0 min. The limits of quantification defined at the signal-to-noise ratio of 10 were 0.17–2.78 ng/mL in rat plasma. The method provided good linearity for the calibration curves with recoveries of 93.3–114.0% and repeatability with relative standard deviation (RSD) of 0.6–5.8% for intra-day and 3.2–10.4% for inter-day measurements. The developed method was applied for supporting the pharmacokinetics investigation of 6-OH-BDE-47 in two groups of Sprague–Dawley rats that received, respectively a single dose of 0.60 mg/kg (high dose) and 0.15 mg/kg (low dose) by intravenous injection. The results showed that plasma levels of 6-OH-BDE-47 declined bi-exponentially with elimination half-life of 71.7 and 85.6 min for lower and higher dose group, respectively. The obtained results of short elimination half-life suggested that 6-OH-BDE-47 might not accumulate significantly in rat.
Keywords: Hydroxylated polybrominated diphenyl ethers; Ultra performance liquid chromatography; Selected reaction monitoring; Pharmacokinetic study;
Development of a reversed phase high performance liquid chromatography method based on the use of cyclodextrins as mobile phase additives to determine pterostilbene in blueberries by Pilar Rodríguez-Bonilla; José Manuel López-Nicolás; Lorena Méndez-Cazorla; Francisco García-Carmona (1091-1097).
In this work, a reversed phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of pterostilbene in food samples. The novel method is based on the addition of cyclodextrins (CDs) to the mobile phase where the complexation of pterostilbene by CDs is carried out. In order to select the most suitable conditions for the RP-HPLC method, the effect of several physico-chemical parameters on the complexation of pterostilbene by CDs was studied. Our results show that the addition of 12 mM HP-β-CD to a 50:50 (v/v) methanol:water mobile phase at 25 °C and pH 7.0 significantly improves the main analytical parameters. In addition, it was seen that pterostilbene forms a 1:1 complex with HP-β-CD, showing an apparent complexation constant of 251 ± 13 M−1. Finally, in order to study the validity of the proposed method, blueberries were analyzed and the concentration of pterostilbene has been determined.
Keywords: Pterostilbene; Cyclodextrins; Reversed phase high performance liquid chromatography; Blueberry;
Determination of 3-methoxysalicylamine levels in mouse plasma and tissue by liquid chromatography–tandem mass spectrometry: Application to in vivo pharmacokinetics studies by Irene Zagol-Ikapitte; Venkataraman Amarnath; Satyawan Jadhav; John A. Oates; Olivier Boutaud (1098-1104).
We report the development of a sensitive liquid chromatography–tandem mass spectrometric assay to quantitate 3-methoxysalicylamine (3-MoSA) in biological samples. Derivatization with 1,1′-thiocarbonyldiimidazole followed by C18 reverse-phase chromatography allowed the detection of both analyte and internal standard (hexylsalicylamine) using electrospray ionization and selected reaction monitoring (SRM) in positive ion mode. We monitored the transitions from m/z 196.7 to 65.1 and from m/z 250.1 to 77.1 for 3-MoSA and HxSA, respectively. The method is validated with respect to linearity (r 2 = 0.995), precision (<17% RSD), recovery (100% for 3-MoSA and HxSA), and stability (77% after storage up to 7 month at −80 °C). The LOD and LOQ were 16.12 and 48.87 μg/l, respectively and the LLOQ of 1 pg/ml. In addition, we used this assay to analyze the pharmacokinetics of 3-MoSA in mouse plasma and tissues following both intraperitoneal and oral administration, providing new information regarding the distribution of this compound in vivo.
Keywords: 3-Methoxysalicylamine (3-MoSA); Hexylsalicylamine (HxSA); LC–MS/MS; 1,1′-Thiocarbonyldiimidazole (TCDI); Mouse tissue; Pharmacokinetics;
Strategies for the purification and characterization of protein scaffolds for the production of hybrid nanobiomaterials by Germán Plascencia-Villa; Jimmy A. Mena; Ricardo M. Castro-Acosta; Julio César Fabián; Octavio T. Ramírez; Laura A. Palomares (1105-1111).
Rotavirus VP6 self-assembles into high order macrostructures useful as novel scaffolds for the construction of multifunctional hybrid nanobiomaterials. This application requires large quantities of high quality pure material with strict structural consistency. Strategies for obtaining high quality recombinant VP6 and different characterization techniques are explored and compared in this work. VP6 was expressed in the insect cell–baculovirus system. VP6 assemblies were selectively purified utilizing an ion exchange and size exclusion (SE) chromatography. Purification steps were monitored and characterized by dynamic light scattering (DLS), ELISA, SDS-PAGE, HPLC and Western blot. DLS showed that the initial ultrafiltration step removed small particles, the intermediate anion exchange chromatographic step completely removed the baculovirus, whereas the final size exclusion chromatography permitted the selective recovery of correctly assembled VP6 nanotubes and discrimination of non-assembled VP6, as confirmed by transmission electron microscopy. VP6 assembled into tubular structures with diameter of 75 nm and several nanometers in length. The purification yield was 20% of multimeric assemblies with a purity >98%. The resulting material was suitable for the production of functionalized hybrid nanobiomaterials through in situ synthesis of metallic nanoparticles.
Keywords: Rotavirus; Nanobiomaterials; Chromatography; Multimeric proteins; Baculovirus;
Automated analysis of mouse serum peptidome using restricted access media and nanoliquid chromatography–tandem mass spectrometry by Geun-Cheol Gil; Jim Brennan; Dan J. Throckmorton; Steven S. Branda; Gabriela S. Chirica (1112-1120).
We demonstrate use of restricted access media with reversed phase functionality (RAM-RP) for analysis of low molecular weight proteins and peptides in mouse serum (75 μl) using a custom designed modular automated processing system (MAPS). RAM-RP fractionation with simultaneous removal of high molecular weight and high abundance proteins is integrated with a follow-on buffer exchange module (BE) to ensure compatibility with subsequent processing steps (trypsin digestion and intact peptide separation prior to mass spectrometric analysis). The high sample capacity afforded by chromatographic methods generates enough sample to achieve comprehensive serum peptidome identification (357 proteins) through tandem mass spectrometric analysis of both intact and digested peptides. Sample losses during transfer between modules are minimized through precise fluidic control; no clogging occurred over several months of serum processing in our low back pressure system. Computer controlled operation of both modules and thorough optimization yield excellent run-to-run reproducibility and protein/peptide overlap in analytical repeats. The robustness of our results demonstrate that the RAM-RP-BE workflow executed on our MAPS platform shows tremendous potential for high throughput peptidome processing, particularly with regard to direct analysis of small-volume serum samples.
Keywords: Proteomics; Peptidomics; Serum; Mouse; Restricted access media; LC–MSMS; On-line processing; Automation;
Studies on the dynamic accumulations of Sophora alopecuroides L. Alkaloids in different harvest times and the appropriate harvest time by Hong-ying Gao; Guo-yu Li; Jin-hui Wang (1121-1125).
A sensitive and accurate method for the simultaneous determination of five alkaloids, namely 9α-hydroxymatrine (M1), matrine (M2), sophoridine (M3), oxymatrine (M4), alopecurin A (M5) in different parts (seed, legume, stem, and root) and different harvest times of Sophora alopecuroides L. was developed by high performance liquid chromatography (HPLC) with photodiode array detector (PDA) for the first time. The separation by gradient elution was achieved on Scienhome Kromasil C18 (4.6 × 250 mm, 5 μm) column at 30 °C with acetonitrile (A)/0.1% phosphatic acid + 0.1% triethylamine (B) as the mobile phase. The detection wavelength was 205 nm. The optimized method provided a good linear relation (r ≥ 0.9993 for all the target compounds), satisfactory precision (RSD values less than 2.3%) and good recovery (96.4–103.6%). The limits of detection ranged between 0.11 × 10−3 and 4.70 × 10−3 μg for the different analytes. The method was successfully applied to analysis and quality control of alkaloid extracts from the traditional Chinese herbal drugs of S. alopecuroides L.
Keywords: Sophora alopecuroides L.; Alkaloids; Harvest time; HPLC;
Chromatography–mass spectrometry studies on the metabolism of synthetic cannabinoids JWH-018 and JWH-073, psychoactive components of smoking mixtures by Andrej Grigoryev; Sergey Savchuk; Aleksandra Melnik; Natal'ja Moskaleva; Jurij Dzhurko; Mihail Ershov; Aleksandr Nosyrev; Aleksandr Vedenin; Boris Izotov; Irina Zabirova; Vladimir Rozhanets (1126-1136).
The Russian Federation prohibited the distribution of herbal mixtures with synthetic aminoalkylindoles JWH-018 and JWH-073, agonist cannabinoid receptors, on January 22, 2010. The lack or low content of their native compounds in urine requires detailed identification of their metabolites, which are excreted with urine and are present in blood. Using gas and liquid chromatography–mass spectrometry, we identified a series of metabolites in urine samples from humans and rats that were products of the following reactions: (a) mono- and dihydroxylation of the parent compounds with hydroxyl groups located at aromatic and aliphatic residues, (b) carboxylation, (c) N-dealkylation and (d) N-dealkylation and hydroxylation. The prevailing urinary metabolites in humans are monohydroxylated forms, while N-dealkylated and N-dealkyl monohydroxylated forms are found in rats. Twenty-six samples of herbal smoking mixtures with JWH-018, purchased in Russia, were analysed.
Keywords: JWH-018; JWH-073; Herbal mixture; Metabolites; GC–MS; LC–MS;
A liquid chromatography assay for a quantification of doripenem, ertapenem, imipenem, meropenem concentrations in human plasma: Application to a clinical pharmacokinetic study by E. Dailly; R. Bouquié; G. Deslandes; P. Jolliet; R. Le Floch (1137-1142).
A simple chromatographic assay based on ultra high performance liquid chromatography with ultraviolet detection at 295 nm is proposed to determinate simultaneously human plasma concentrations of imipenem, doripenem, meropenem and ertapenem. After deproteinization by acetonitrile, carbapenems are separated on a PentaFluoroPhenyl column with a binary gradient elution. This method is specific, accurate, precise (the intra-day and inter-day imprecision and inaccuracy are lower than 15%), sensitive (the limit of quantitation is equal to 0.50 mg/L for imipenem, doripenem, ertapenem, meropenem) and not time consuming (run time = 7 min). An application of this method to measure ertapenem plasma concentrations in burn patients is presented.
Keywords: Doripenem; Ertapenem; Meropenem; Imipenem; Ultra high efficiency chromatography;
Electromembrane extraction of trace amounts of naltrexone and nalmefene from untreated biological fluids by Maryam Rezazadeh; Yadollah Yamini; Shahram Seidi (1143-1148).
Nalmefene and naltrexone are used to block the effects of narcotics and alcohol. In the present work, for the first time a microextraction technique was presented to reduce matrix interferences and improve detection limits of the drugs in urine and plasma samples. Electromembrane extraction (EME) followed by high performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection was optimized and validated for quantification of nalmefene and naltrexone from biological fluids. The membrane consists 85% of 2-nitrophenyl octyl ether (NPOE) and 15% di-(2-ethylhexyl) phosphate (DEHP) immobilized in the pores of a hollow fiber. A 100 V electrical field was applied to make the analytes migrate from sample solution with pH 2.0, through the supported liquid membrane (SLM) into an acidic acceptor solution with pH 1.0 which was located inside the lumen of hollow fiber. Extraction recoveries in the range of 54% and 75% were obtained in different biological matrices which resulted in preconcentration factors in the range of 109–149 and satisfactory repeatability (2.0 < RSD% < 8.3). The method offers good linearity with estimation of coefficient higher than 0.9946. Finally, it was applied to determination and quantification of drugs in human plasma and urine samples and satisfactory results were yielded.
Keywords: Nalmefene; Naltrexone; Electromembrane; Microextraction; Urine; Plasma;
Validation of high-performance liquid chromatography–tandem mass spectrometry assays for the quantification of eribulin (E7389) in various biological matrices by A.C. Dubbelman; H. Rosing; B. Thijssen; L. Lucas; W. Copalu; J. Wanders; J.H.M. Schellens; J.H. Beijnen (1149-1155).
This paper presents specific and sensitive high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC–MS/MS) assays for the quantification of the novel anticancer agent eribulin in human plasma, whole blood, urine and faeces. These assays, developed to support clinical pharmacological studies with the drug, quantify eribulin concentration ranges of 0.2–100 ng/mL for plasma, 0.5–100 ng/mL for whole blood and urine and 0.1–25 μg/g for faeces, using sample volumes of 500 μL or 250 μg (faeces). Samples were prepared with liquid–liquid extraction, separated on a C18 column with gradient elution and analysed with a triple quadrupole MS, in positive ion mode. A structural analogue of eribulin was used as internal standard for the quantification. The assays were linear with correlation coefficients (r 2) of 0.99 and better, whereby the deviation from nominal concentrations ranged from −8.2 to 8.9% with CV values of maximally 14.2%. Stability assessments demonstrated that eribulin is stable at −20 °C in plasma, whole blood, urine and faeces for at least 38, 4, 10.5 and 5 months, respectively. In conclusion, the validation results show that the assays are specific and accurate and can therefore adequately be applied to support clinical studies of eribulin.
Keywords: Eribulin; E7389; LC–MS/MS; Human mass balance study;
Corrigendum to "Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry" [J. Chromatogr. B 878 (2010) 3303] by Omnia A. Ismaiel; Tianyi Zhang; Rand G. Jenkins; H. Thomas Karnes (1156).


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Pearl Nickel
Our range of pearl nickel switches and sockets are valuable home décor accessories as well as functional electrical components. They come in square and rectangular plates with rounded corners and a variety of different switches and sockets. This type of pearl nickel finish offers a clean simple design that can fit in any room of a home or business. Given that they can feature different switches such as standard light switches, oven switches and dimmer lights, and sockets such as standard plugs, co-axial and telephone sockets, this design versatility is a great strength.
Nickel is tougher than PVC and plastic which makes them more durable and long lasting, which in turn makes them more cost-effective. Pearl nickel switches and sockets are also easy to maintain because they do not spoil or stain as often as other types of electrical components. Cleaning them is a simple process, with a clean dry tissue or cloth sufficient to clean them in most cases.
The inner workings of pearl nickel switches and sockets are no different from other standard sockets and switches. As with all the other trusted electrical equipment we offer, our pearl nickel range are all rated at 13A, designed to meet all current UK electrical safety regulations. So, whether you need a cooker control unit, a shower heater switch, a two-way lighting system or a double socket outlet, all these can be catered for with items from our ranges of pearl nickel switches and sockets.
At Electrical Warehouse, we sell only the highest quality pearl nickel switches and sockets for your operational needs. Whether you are a full-time trades person, or a homeowner doing DIY, we have the ideal items for you. Take a look through our pearl nickel range and if you find the right one for you, it's simple to place an order and enjoy our great savings and next day delivery deals.
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Polaroid XS100 Extreme Edition HD 1080p 16MP Waterproof Sports Action Video Camera With Full Mounting Kit Included by Polaroid – If you cringe at the price of some of the other products like this, we all understand. That is why the XS100 is so nice, it does the same thing at a much cheaper price. We have put it on bikes, on helmets, used it to film underwater and it has done the job. The kids just LOVE being able to film themselves in the water and it does double up as a nice training aid for your budding swim team member. This is one of those products that makes it on our vacation list as we don't have to worry if it goes on the beach or makes it way to the water, the pictures and the videos still come out looking great.
Toys And Games
2013 Holiday Gift Guide 8 and 10 Year Old Boys
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Toys And Games


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Pete Davidson's Tattoo Artist Says He and Ariana Grande Are "Super in Love"
Ariana Grande and Pete Davidson are legit in love, according to Davidson's tattoo artist, Jon Mesa, co-owner of No Idols tattoo shop in New York City.
In May, Mesa says Davidson came in to have a tattoo of his ex-girlfriend, Cazzie David, covered up, prompting Mesa to dole out some advice.
"So I did a huge coverup [adding a forest of trees]…After we were done, I told Pete, 'Hey, dude, let's just stop with the girlfriend tats until after [Grande's] your wife,'" Mesa told Page Six. "Because relationships come and go, and Pete is a really young man. [But] I think that went in one ear and out the other."
On the one hand, Davidson did not wait until he was married to Grande to get some ink in her honor (earlier this month, he got her initials tattooed on his thumb and her iconic Dangerous Woman bunny mask inked behind his ear). On the other, he didn't wait long after getting those tattoos to pop the question, so many he took some of Mesa's advice to heart?
Davidson went to Los Angeles-based tattoo artist London Reese for his first two Ariana-themed tattoos, but he returned to Mesa for a third after that—he wanted the word "Reborn," to symbolize how much his life has changed since meeting Grande. In addition to adding to Davidson's collection of ink, Mesa got the chance to see the SNL star and the singer together because she accompanied him for the tat.
So what are Pete Davidson and Ariana Grande like as a couple?
"They are just riding that magical wave of bliss together," he said. "It's obvious that Pete is super in love with Ariana."
Super in love, guys.


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Fort Worth 4521 south hulen St Suite 200. Fort WorthTX, 76109 817-386-8085. line. dallas 5429 lbj Freeway, suite 100. dallas, Texas 75240 214-251-8001.
Texas Department of Housing and Community Affairs – Mortgage Credit Certificate: The Texas Mortgage Credit Certificate provides qualified borrowers with up to $2,000 per year in a federal income tax credit based on mortgage interest paid in the tax year. Applicants must be first-time homebuyers and must meet income and purchase price limits.
Current Us Interest Rate Inevitable because the current amount of negative-yielding. negative-yielding debt.. this imminent slide in interest rates will turn the rest of the global financial system Danish, giving us bank.
Mortgage rates change often, but the data for Texas show a considerable range of quotes. In real terms, a home loan at the state's lowest reported interest rate has a monthly cost of $697. This is significantly lower than the highest rate, which costs $1,038 each month.
Finding the Best Mortgage Rates in Fort Worth, TX. Most of the population does not have enough cash on hand to buy a house outright. A loan is most likely one of the first priorities when one is looking to purchase their house in Fort Worth, TX. Even houses that start at $50,000 will require being approved for a loan for most people.
Despite low mortgage rates, high levels of student loan debt are preventing potential. In addition to existing homes,
Mortgage Interest Rate in Percentages (%), Yearly, for 20 Years Fixed-Rate : 4.25: 3.17-4.54: Prices in Fort Worth, Texas. This city had 84 different contributors in the past 18 months. Some data are estimated due to a low number of contributors. If you are living here, please update our data.
Mortgage Rates | Dallas TX Homes for Sale and Real Estate. Marilyn. l Atlanta 404-414-0690 l Hill Country TX 512-970-8601 l Fort Worth TX 817-996-8080.
Rates for home loans were little changed in the April. on refinances to make up for lean purchase activity. People are flooding into Texas metros – Dallas-Fort Worth picked up 123,661 in 2016, an.
The Best Mortgage Lender in Fort Worth, Texas. The texas mortgage pros team consists of the best mortgage lenders in Texas.We are committed to providing our San Antonio, Texas clients with the highest quality service for your mortgage needs.
Interest Only Refinance Rates NerdWallet's comparison tool can help you find the current refinance rates for your mortgage. In the "Refine results" section, click or tap the "Refinance" button and enter a few details about.
Find Dallas / Fort Worth, Texas homes for sale. You can count on the realtors at RE/MAX DFW Associates for all of your Texas real estate needs!
Interest Rate For Fha Loan How To Check Mortgage Rates Daily Current interest rate fha loan – A common misconception of the FHA loan program is that the FHA or HUD is responsible for setting interest rates on FHA guaranteed home loans. The FHA does place limits on certain fees, how closing costs and down payments are paid and by whom. The FHA does regulate (but does not set) interest rates in some cases.Today's Mortgage Rates Who Determines Interest Rates? Interest rates are typically determined by a central bank in most countries. In the United States, a forum is held once per month for eight months out of the year to determine interest rates.Aron of Hunington Properties, Inc. The FHA-insured financing for the property located at 1900 Kilgore Parkway is a ground-up construction loan that converts to a 40-year permanent, fixed-rate,


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· home equity loans – Rates are based on a fixed rate home equity loan in Texas for an owner occupied residence, second lien, 10 year or 15 year repayment terms with an 80% loan-to-value ratio for loan amounts of $50,000. Rate Discount indicates the amount of reduction in the Rate for having monthly payments automatically deducted from an account and/or for having other relationship.
Home Equity Loan: As of August 31, 2019, the fixed Annual Percentage Rate (APR) of 4.89% is available for 10-year second position home equity installment loans $50,000 to $250,000 with loan-to-value (LTV) of 70% or less. Rates may vary based on LTV, credit scores, or other loan amount.
Home equity is great for homeowners looking to take out a low interest. that using a HELOC to pay off credit card debt can be a good idea.
Home Equity Loan In Texas IRVING, Texas–(BUSINESS wire)–american home mortgage servicing, Inc. wishes to issue the following. of AHMSI's residential servicer rating for prime, Alt A and home equity lines of credit to.Home Equity Loan Calculator Chase NEW YORK (MarketWatch) — Strength from its investment banking arm pushed first-quarter profit at J.P. Morgan Chase up 55%. on consumers continued to drive losses for the mortgage and home equity.
As home prices continue to climb, home equity loans and lines of credit are becoming potential sources of extra cash for a growing number of homeowners.
A Brief History of Home Equity Loans in Texas. In 1997, the Texas Constitution's Section 50 gave home equity lenders the right to operate within the state on a limited basis. Section 50's provisions are exceedingly strict, and lenders that fail to follow them can face serious penalties. In the most extreme case,
For Texas primary residences, we will lend up to 80% of the total equity in your home and your line of credit amount cannot exceed 80% of the home's value. For line amounts greater than $500,000, maximum combined loan-to-value ratios are lower and certain restrictions apply.
Home equity loans are paid in full over the life of the loan, in equal monthly payments that contain both principal and interest. For both home equity products, you can always make additional payments toward principal. Use our calculator to help estimate your monthly payment.
Texas Home Equity Loan Calculator With a Chase home equity line of credit (HELOC), you can use your home's equity for home improvements, debt consolidation or other expenses. Before you apply, see our home equity rates, check your eligibility and use our HELOC calculator plus other tools.
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Kampala, Uganda | THE INDEPENDENT | Commercial banks in Uganda are looking into how they can beef up the security of their agents due to the risk exposed to them for holding cash. This comes after an incident on 30 June 2019 in Nansana where three guards attached to Tiger …Read More »
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Afreximbank seeks to recruit 700 banks as agents
Kampala, Uganda | ISAAC KHISA | Cairo-based African Export-Import Bank (Afreximbank) is seeking to recruit at least 700 commercial banks as agents across the continent to extend financial services to Small and Medium Enterprises (SMEs). Amr Kamel, the Bank's Executive Vice President, Business Development and Corporate Banking, said the multi-lateral lender …Read More »


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- Latest available (Revised)
- Original (As made)
This is the original version (as it was originally made).
5.—(1) A person (A) is entitled to welfare supplementary payment under this Part if—
(a)A is a member of a couple with another person (B),
(b)A meets the IS entitlement condition,
(c)A meets the disability-related premium entitlement condition in respect of disability premium or enhanced disability premium,
(d)A or B meet the PIP refusal condition, and
(e)A meets the termination of disability-related premium condition.
(2) The IS entitlement condition is that the person is in receipt of income support on the transition date.
(3) The disability-related premium entitlement condition in respect of disability premium or enhanced disability premium is that the person is in receipt of—
(a)disability premium under paragraph 11,
(b)enhanced disability premium under paragraph 13A,
of Schedule 2 to the Income Support (General) Regulations (Northern Ireland) 1987, on the transition date.
(4) The PIP refusal condition is that following a transitional assessment determination, the person is refused personal independence payment, or the component of personal independence payment, that is a condition for entitlement to disability-related premium.
(5) The termination of disability-related premium condition is that, in consequence of the PIP refusal condition, the decision of the Department to award disability-related premium is superseded by a decision to not award disability-related premium.
Latest Available (revised):The latest available updated version of the legislation incorporating changes made by subsequent legislation and applied by our editorial team. Changes we have not yet applied to the text, can be found in the 'Changes to Legislation' area.
Original (As Enacted or Made): The original version of the legislation as it stood when it was enacted or made. No changes have been applied to the text.
Explanatory Memorandum sets out a brief statement of the purpose of a Statutory Rule and provides information about its policy objective and policy implications. They aim to make the Statutory Rule accessible to readers who are not legally qualified and accompany any Northern Ireland Statutory Rule or Draft Northern Ireland Statutory Rule laid before the UK Parliament during the suspension of the Northern Ireland Assembly.
Access essential accompanying documents and information for this legislation item from this tab. Dependent on the legislation item being viewed this may include:
Use this menu to access essential accompanying documents and information for this legislation item. Dependent on the legislation item being viewed this may include:
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We are happy to make returns of your unwashed and unworn items if you are not fully satisfied with your purchase.
Rushmore requires an original receipt and the return must be within 14 days in order to receive your original form of payment. If the 14 day threshold has passed but you are still within 30 days of your purchase,
accompanied by a receipt, then you may return the item for store credit only.
Rushmore accepts all major credit cards and cash as payment.


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Live Irrésistible Rosy Crush is a confident explosion of pink freshness and bold vitality. A vibrant floral, bursting with rose and vivacious pink pepper.
Goji berries and chypre-patchouli blend youthful vivacity with elegant sophistication. A bouquet of pink rosiness in many shades. Because pink is audacious, poetic and pure; pink is a positive state of mind. A fragrance for women who live irresistibly in the moment and embrace the power of pink.
Pink is more than a colour, it's a positive state of mind.
Shop with confidence at World Duty Free
World Duty Free offers exceptional year-round value across its entire product range, so its promise to you is that if you purchase anything from World Duty Free and then find the identical item cheaper on the UK high street within the next 28 days, it will happily refund the difference.
World Duty Free regularly surveys the prices of its fragrance, cosmetics, skincare, spirits, wine and champagne range on the UK high street. An independent company travels the country to find the brands stocked in its airport stores in the shops you typically buy from week-in week-out. It uses these observations to create an Average UK High Street Price; this is the comparison price it shows its savings against.
Typically you will enjoy 20% off the Average High Street Price (where surveyed) on all of World Duty Free's fragrance and non-vintage champagne. You can save up to 50% off selected spirits and you will save the equivalent of VAT on cosmetics, skincare, sunglasses*, watches* and jewellery* (*savings compared to RRP)


text: Graphics Graphics The industry lacks hundreds of thousands of coders, and wages keep rising. Be a hero!Discover more... Save the industry Home Want to work from home? We got your back! Forum Graphics and designObecná diskuze o tvorbě grafiky pro web / programy / hry. Adobe Photoshop, Adobe Illustrator, 3D studio MAX FreeUnratedWritten by David Capka Activities (1)


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Have Your Online Passwords Been Stolen? Here's How to Find Out.
Data breaches have become commonplace, with online thieves constantly breaking into corporate and government servers and making off with millions—or even hundreds of millions!—of email
Did You Know You Can Customize the Columns in a Finder Window's List View?
When a Mac folder contains a lot of files, the Finder's List view often works best, since it lets you focus on a single folder
Sneak Preview of What's Coming from Apple This Fall
At Apple's Worldwide Developer Conference keynote on June 4th, the company unveiled the first developer versions of all four of its operating systems: macOS 10.14
Apple Introduces New 6th Generation iPad with Apple Pencil Support, Updates iWork
At a special education event on March 27th, Apple introduced a new 9.7-inch iPad that offers faster performance, support for the Apple Pencil, and a
Use Copy as Pathname to Help Someone Find a File on the Mac
Have you ever needed to write directions for where to find a file on the Mac? That's easy if it's in a well-traveled location, like
How to Deal with macOS Server Losing Many of Its Services
For many years, Apple has sold macOS Server (previously called OS X Server) for those who wanted to run various Unix-based Internet services on a


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Press Release
Mountain Line | 420 DuPont Road | Morgantown, WV 26501 | (304) 296-3680 | (304) 291-7429 | www.busride.org
For Immediate Release
Date: October 14, 2019
Contact: David A. Bruffy, General Manager
Phone: (304) 296-3680
The Mountain Line Transit Authority is pleased to recognize outstanding employee performance for the month of September, 2019. After evaluation of 37 Drivers in 10 performance categories, the Authority is pleased to recognize Tom Jones as Driver of the Month for September, 2019. The Authority also recognizes Robert Smith, Michael Kingan, John Blosser and Robert Lee for their top performances in September.
All of these employees are being recognized for their demonstrated driving skills, safety, public relations, assistance to fellow drivers and maintaining route schedules in the course of their daily performance.
For more information about the Mountain Line Transit Authority, call 304- 291-RIDE (7433) or visit our web page at WWW.BUSRIDE.ORG.
End Release –
Thank you.


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QualificationsBachelor's degree, preferably in business management or a related discipline, or equivalent work experience required, advanced degree preferred. Project Management Professional (PMP) certification is beneficial 10 - 12 years of related project management experience required, experience with technology or financial services industry is a plus
BNY Mellon is an Equal Employment Opportunity/Affirmative Action Employer.
Minorities/Females/Individuals With Disabilities/Protected Veterans.
Primary Location: United States-New York-New York
Internal Jobcode: 45190
Job: Information Technology
Organization: Architecture and Data-HR16450
Requisition Number: 1917394


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Problems installing 10.04 with Intel H55 chipset
System boots CD, creates partition, installs OS. After reboot, allows me to log in, and then nothing... shows the basic background, but no taskbar, no icons... Can reboot and repeat all day long. I know from other posts that Intel boards take a bit of massage to get working, but I have never seen anything for this chipset.
Question information
- Language:
- English Edit question
- Status:
- Solved
- Assignee:
- No assignee Edit question
- Solved by:
- Jim Cobb
- Solved:
- 2010-09-15
- Last query:
- 2010-09-15
- Last reply:
- 2010-09-14
If you boot to root recovery mode then run:
cd /home/foo; rm -r .g*; rm -r .config
replace foo with your username you setup at install time. Reboot and log in. Should be ok.
|Jim Cobb (jim-cobb) said :
I will give it a shot...
|Jim Cobb (jim-cobb) said :
Actually, it did not work. I did not have a .g ... But it gave me the means figure out the real issue. I have it hooked up to a 46" Samsung, set to wide mode. I just couldnt see the taskbars. (duh) It was working properly all of the time. Though I have found that I can not size my screen to not do that. I am going to see if I can get drivers for that screen. Right now, it is showing up as a Samsung 7" screen.
Thank you for your assistance...