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– [Kyle] Grand Theft Auto
has been around for– (phone rings) Oh, sorry about this. (phone beeps) Hello?
(static crackles) What? Bowling? I can't, Cousin, you know I can't today. I'm busy going through the timeline of Grand Theft Auto's HD Universe. (static crackles) No, not San Andreas and Vice City, they're in the 3D Universe
and I already covered that. The HD Universe covers GTA IV, Chinatown Wars, GTA V and their DLC. (static crackles) Yeah, okay, sure, no,
I'll cover GTA Online too. Look, I'll call you back, okay? – Okay.
– Okay. (phone beeps) Sorry about that, let's get started with the man himself, Niko Bellic. (contemplative synth music) Serbia: Mid-late 1990s to 2000s. As a soldier during the Yugoslav
Wars, Niko sees and does some of the worst things
humanity is capable of. He and a group of his fellow soldiers get ambushed, and only
three of the 15 survive: him, Florian Cravic and Darko Brevic. Niko figures one of
these two sold them out, so he vows revenge for his fallen friends. After the war, Niko starts
working for Ray Bulgarin and his human trafficking ring. While on a smuggling run to Italy, Niko sabotages the ship he's on, steals a bunch of Bulgarin's
money, and swims to safety. But Bulgarin has eyes all
over Europe, so Niko figures the only way to be safe
is by escaping overseas. While all this is going
down, over in America, three ambitious criminals have a plan. 2004, North Yankton. Michael Townley, Trevor Philips, and Brad have their eyes set on a load of cash held by Bobcat Security in North Yankton. They take hostages and successfully steal nearly $200,000 from the vault. They make a break for their getaway car and ride off, but this
is Grand Theft Auto. The police are everywhere, even when you don't think they are. The cops chase the robbers,
and a bullet strikes their driver, forcing Michael to drive. He loses the cops and then
crosses some train tracks. As he does, a train nicks
the tail of the car, causing it to spin out
and smash into a tree. The trio proceed on foot,
but they're intercepted by FIB Agent Dave Norton, who shoots Brad. A second shot rings out, Michael falls to the ground and tells Trevor to run. So the last man standing retreats into the snowy fields of North Yankton. Fast-forward to Michael's funeral, and Norton walks by the
proceedings, cigarette in hand. Then, just beyond the fence, Michael, sporting a beanie and aviators, puts out a cigarette, very much alive. Dun, dun, dah! 2008, Liberty City: Enter Niko Bellic. We jump to 2008, and this is a doozy of a year in the GTA canon. We'll be moving pretty freely
between Grand Theft Auto IV, The Lost and the Damned,
and The Ballad of Gay Tony to tell the whole story as best we can. After stowing away on a ship
for seven months, Niko Bellic finally arrives on the
East Coast in Liberty City. He meets with his cousin Roman, who talked up his lavish
life in Liberty City. Niko quickly learns that Roman owns a struggling cab business
and can't make ends meet. Loan sharks are breathing down his neck, so Niko helps him with a few jobs. One job in particular leads to Niko chauffeuring Mallorie and
Michelle, both of whom would eventually date Roman
and Niko respectively. From there, Niko networks in Liberty City, meeting Little Jacob, the
head of the Jamaican Mafia. The two become quick friends,
but things are way more tense between Niko and Vlad,
Roman's Russian loan shark. Niko inevitably executes Vlad
for sleeping with Mallorie, getting revenge for his cousin. Hey, speaking of revenge, at this point, Niko reveals why he's in America, to find his old squadmates
during the Yugoslav War and get answers, and revenge. He's just on a revenge spree, just lot of revenge in this story. Soon after revealing
this, the Bellic cousins are kidnapped by one of
Mikhail Faustin's goons. Who's Mikhail Faustin? Just your run-of-the-mill Russian mob boss bent on achieving his own
version of the American dream. We've seen it a million times. When the cousins wake
up, they meet Faustin and his colleague, Dimitri Rascalov. The mobsters coerce Niko into
doing some work for them. One of these jobs sends Niko to Broker to kill Jason Michaels, a member
of the Lost Motorcycle Club who has been seeing Faustin's daughter. Michaels was small time, but
Faustin starts asking Niko to do more dramatic hits, which forces Dimitri to put a hit on Faustin. Niko carries it out and reports back to Dimitri to claim his reward. But in a not-so-surprising twist, Dimitri betrays Niko,
selling him back to Bulgarin, the man Niko escaped from in Yugoslavia. Thankfully, Little Jacob saves Niko, and they fight off
Bulgarin's men together. After that, Dimitri doubles down and sets fire to Roman's apartment
and budding cab business, forcing the cousins to retreat north to Bohan and meet up with Mallorie, who knows a few people in
the area who can get them some work, like local drug
dealer Elizabeta Torres. Niko goes on a slew of different jobs, making all kinds of friends along the way including Dwayne Forge,
Playboy X and Brucie Kibbutz. Eventually, in one mission, Niko watches over a huge heroin deal, but
things don't go as planned. But when do they do in the
Grand Theft Auto universe? Tell me that, when do
they go according to plan? The Botched Heroin Deal. So let's rewind a bit. While Niko was getting acquainted
with the Russian mafia, Johnny Klebitz was dealing
with his own affairs. He's the vice president of
the Lost Motorcycle Club, and for a while now, the
gang has had some beef with its rival, the Angels of Death. The tipping point came when Billy Grey, the chapter president, heard
that one of their boys, Jason Michaels, was killed in Broker, which might sound familiar to us because we know that that's
Niko who gunned him down. We know the truth, but
Billy spins the story and blames it on the Angels instead. At this point, Johnny starts
butting heads with Billy, and even though he knows
it's a bad idea, he joins the Lost in attacking the
Angels, stealing some heroin. Johnny meets up with
Elizabeta to sell the heroin at a house party, where
he also meets Niko. Unfortunately, the buyer turns
out to be an undercover cop, and the whole party ends with a firefight. The Lost later try selling
the heroin to the Triads, but that deal doesn't go as
according to plan either, leading to another firefight. The Grand Theft Auto universe
seems to have a lot of these. Billy ends up getting arrested, making Johnny the de
facto chapter president. To keep the gang in the green, he starts doing some odd
jobs for other criminals. One of these leads to him and his friend Malc kidnapping Roman. You can imagine Niko was
not happy about this. Luck of the Irish. Well, after the botched heroin deal, Niko moves onto bigger, better things, eventually meeting local
criminal Packie McReary. He still pushes drugs, too,
and Elizabeta calls Niko, claiming the Angels ripped
her off in a cocaine deal. He meets up with Little
Jacob to steal the cocaine, and after they retrieve it,
they run into Niko's girlfriend, Michelle, except her name isn't Michelle. Her real name is Karen Daniels, and she's been working undercover for the International Affairs
Agency the whole time. She takes the coke from
them and offers Niko a deal. If he comes and works for
the IAA, he won't go to jail. Don't be mistaken though, Niko's
still a criminal at heart. He works with Packie to
commit some lucrative crimes, some of which were under
the orders of Ray Boccino. He's living his best
life until he gets a call from Mallorie and learns that Dimitri and the Russians have kidnapped Roman. No one mentions Johnny's involvement, so let's keep that a secret
between you and me, okay? Niko saves his cousin, while his hatred for Dimitri continues to grow. Some time afterwards,
Niko works with Packie to rob one of the larger
banks in Liberty City. They then take hostages in the process, and one of which is our
third GTA IV protagonist. A Criminal's Best Friend. So let's set the stage one more time and talk about Luis Fernando Lopez. We first meet Luis with
his men on the ground of a Liberty City bank
during Niko's robbery. When he is not fearing for his life, he works as the right-hand man of Anthony Gay Tony Prince,
a well-to-do nightclub owner who isn't doing so well anymore. Tony has been losing money, so he works with Luis to make some shady deals. One of these deals is with a character simply known as the Cook. This guy came to Liberty City on the same boat Niko
did earlier in the year. See, he stole some diamonds from Bulgarin before going to America and planned to sell them to Tony and Luis. But Boccino sent Johnny to ambush the deal and steal the diamonds before
they could make the exchange. Meanwhile, the McReary family hires Niko to kidnap the daughter
of Giovanni Ancelotti, the don of the Italian mob,
and hold her for ransom. Ancelotti believes Gracie's kidnapping has to do with the diamonds,
so he blames Tony and Luis. Boccino actually has the diamonds, so he plans to sell
them to the Jewish mob, and he tells Johnny and
Niko to oversee the deal. Ancelotti catches wind of this deal and sends Luis to crash it. In the end, Luis walks
away with the diamonds, Johnny has all the money,
and Niko gets nothing. Black and white, clear as crystal, he gets nothing, he loses, good day, sir. While all the hubbub with the diamonds is going on, Boccino tells Niko where one of his old
squadmates is holed up. He tracks down Florian Cravic, which now goes by Bernie Crane. Bernie claims he didn't betray the squad, so Niko narrows it down to one
final suspect, Darko Brevic. With some help from Karen and the IAA, Niko tracks him down and
kills him, or spares him, depending on what you, the player choose. Meanwhile, Johnny turns his attention back to his old friend, Billy. He's been in prison and is gonna rat on the rest of the Lost
MC to reclaim his freedom. So Johnny and his gang
break into the prison to personally execute Billy. The bikers then torch
their base in Liberty City, tying up all the remaining loose ends. Now, back to the diamonds,
Ancelotti forces Tony and Luis to trade the diamonds
for his daughter's life. Packie and Niko show
up to hand Gracie over, but partway through the transaction, Bulgarin and his men arrive. Another firefight breaks out, and one of Bulgarin's men
ends up throwing the diamonds into a dump truck as it drives away. The diamonds are completely
out of the picture, so the Ancelotti family contacts Luis to have him kill Tony over the lost goods. Instead, his loyalty
for Tony shines through, and the two cut ties with the mobsters. Luis also assassinates Bulgarin, shutting him up permanently,
and he reunites with Tony, and the two celebrate,
giving this town the finger. Meanwhile, Jimmy Pegorino,
another Italian mobster, hires Niko to assassinate
Boccino, and now all the pieces are coming together for the end of GTA IV. Deal or No Deal. Pegorino asks Niko to conduct
one final heroin deal. Niko hesitates though because
Dimitri Rascalov is involved. And here's where the story branches. If Niko takes the deal, Dimitri will double-cross
him and try to murder him. Niko survives and vows to get
his revenge on the criminal. He attends Roman and
Mallorie's wedding ceremony, and one of Dimitri's assassins
will try to kill him again. Niko survives, but in the
scuffle, Roman gets fatally shot. With Little Jacob's help,
Niko hunts down Dimitri and ends him once and for all. Finally, Niko gets a phone call from Mallorie, who reveals she's pregnant. He promises to do his best to be a father figure for the child. If Niko instead decides
to skip out on the deal, he'll betray Pegorino's trust. He ambushes Dimitri and murders him. Afterward, he attends Roman
and Mallorie's wedding ceremony with Packie's sister,
Kate, as his plus one. She's a genuinely good person, they've been dating for a
little while, but just as Niko's ready to put his criminal ways behind him, Pegorino drives by the
church, fatally shooting Kate. Niko, along with Jacob and Roman, chase after the crime boss, eventually assassinating
him on Happiness Island. This is considered the canon ending, since there's hints of
Roman being alive in GTA V. And if Roman's alive, then that means, yeah, you can figure it out
by process of elimination. 2009, Liberty City. A year after Niko's antics,
some trouble with the Triads start to flare up in Liberty City. Chinatown Wars follows
the story of Huang Lee. His father, a Triad boss,
mysteriously died recently. He intends to deliver Yu Jian,
his father's prized sword, to his uncle Wu Kenny Lee, so
Kenny could hand the sword off to Hsin Jaoming, Liberty
City's current Triad Boss. Unfortunately, someone steals the sword from Huang the moment he
lands in Liberty City. A corrupt police officer named Wade Heston offers Huang a deal, and
the two team up to find out who stole Yu Jian and who
murdered Huang's father. At first, Huang investigates
both the Angels of Death and the local Korean mob,
but neither were seen guilty. He does, however, find out that there's a traitor among the Triads who has been leaking
information to the police. Huang turns his investigation
towards the FBI, hacking into their servers
to see who the snitch was. He finds out that two
high-ranking Triad officers have talked to the police, one
of whom is the son of Hsin. Dishonored by his son, Hsin resigns, opening the path for Kenny to
become the new Triad leader. Huang executes the two
traitors, but soon after, Heston finds out that
the information was fake. Turns out that the real traitor was none other than Uncle Kenny,
who also killed Huang's father. Hsin confronts Kenny,
demanding he return Yu Jian, so Kenny obliges and he
gives him the sword back by putting it straight through him or kind of slashing him with it? I don't know, cut scenes on the DS and PSP aren't the most detailed. The point is, Huang kills his uncle Kenny and avenges his father. As he is lying in a pool
of blood, Hsin suggests that Huang could lead
the Liberty City Triads. But just then, the authorities
bust in and arrest him. Heston vouches for Huang, so
he does get off scot-free. 2013, Los Santos: GTA Online. Now let's head out west to the
land of Vinewood: Los Santos. Merryweather, a private military company, sets up in the area, bringing all sorts of
weapons and vehicles. Your GTA Online character
lands in the city to meet with Lamar Davis, a close
totally-not-Facebook friend. Lamar introduces you to a
variety of criminal contacts, and your network includes
characters like Simeon Yetarian, Trevor Philips, Lester
Crest, and Martin Madrazo. Your character becomes a criminal mongol, running all sorts of heists
for Lester and Trevor while owning more organizations
than you can count. You can even start your own
biker gang courtesy of Malc, the guy who kidnapped
Roman back in Liberty city. Your organization specializes
in contraband, narcotics, and all other kinds of seedy businesses. Over the course of your criminal career, you steal and destroy a variety of weapons and intel controlled by Merryweather. You keep in touch with Lester, who plays a major role with the main
trio of Grand Theft Auto V. A few years down the line,
your character even opens a nightclub with the help of Gay Tony. Look, there's lots to do in GTA Online, and it's always getting updated. I'm sure its story will keep
developing beyond this video, so let's move on to the
twisting story of GTA V. Michael, Trevor, and Franklin. Thanks to the witness protection program, Michael De Santa, aka Michael Townley, has a not-so-happy life in Los Santos with his wife and two kids. Elsewhere, Franklin Clinton
and his friend Lamar Davis get by doing odd jobs, like repossessing cars
for Simeon Yetarian. One of the cars ends up being Michael's, but instead of being angry, Michael turns out to like
the cut of Franklin's jib. The two develop a
mentor-student relationship that culminates in them destroying a house that belongs to Martin
Madrazo, a drug cartel leader. Suddenly, Michael owes
Madrazo a lot of money, so he slips back into his old
criminal ways to get the cash. He then contacts Lester Crest to set up a jewelry store
heist to kickstart that, you know, just the old-fashioned
way of kickstarting. Lester forms the team,
and he says he considers a eastern European guy
making moves in Liberty City as well as a local contact
who's too unpredictable, clear references to Niko Bellic and your GTA Online character. Anyway, even without Niko,
because he is the best, the heist is a success. Up north in the desert, we find Trevor and Johnny Klebitz's girlfriend. Since 2004, he figured that Brad went to jail and Michael died. But when he sees the
jewelry heist on the news, he recognizes Michael's handiwork. Before heading into the
city, he murders Johnny and some other Lost MC members to monopolize the meth
production in the area. Trevor meets Michael in Los Santos, but he's furious that his
old friend is still alive. Despite their differences, the
two end up working together to save Michael's daughter
from public embarrassment. Norton, the FIB agent who cut Michael the witness protection deal,
knows that Michael committed the heist, and Trevor's presence
makes him uncomfortable. He blackmails Michael
into doing some dirty work to undermine the
International Affairs Agency. Sure enough, Michael eventually gets Franklin and Trevor
involved in the same mess. Michael's family moves out of his mansion after seeing he's become a criminal again. Unfortunately, he's in too deep now. FIB Agent Steve Haines ropes the trio into a series of crimes,
one of which has Michael crossing paths with Karen Daniels. Along the way, they make contact with Devin Weston, a local billionaire. Who gives the trio a few more missions revolving around stealing rare cars, which puts Michael, a
longtime movie-lover, in contact with producer Solomon Richards. Thanks to his new
connection, Michael becomes a producer on an upcoming film. Madrazo hires Michael and Trevor to silence someone who
can testify against him. After the hit, Trevor
reports back to Madrazo, but the crime boss refuses to pay, which pushes Trevor to
kidnap his wife, Patricia, and promptly fall in love with her. He also steals a bunch
of weapons and stuff from Merryweather, the military company. Eventually, Michael gets back
on Mr. Madrazo's good side by bribing him with a rare Aztec artifact, and Trevor returns
Patricia to the crime boss. Michael and Trevor start planning a big heist at the Union Depository, one of the biggest banks in the nation. However, when they travel to
North Yankton, Trevor finds out that Brad was buried in
place of Michael Townley. He and Michael ultimately
try to kill each other, but some Triads show up
to try to kill Trevor. Trevor gets away, so they
kidnap Michael instead. Trevor leaves Michael to die, but Franklin eventually rescues him. Now a free man, Michael
reunites his family, aggressively so, at the behest of his son. He then hears that Weston plans to shut down Solomon's studio,
which would eventually kill the movie Michael helped produce. In the heat of the
moment, he saves the movie by stealing the film reel,
leaving Weston's assistant dead. After tidying up his personal life, Michael gets back down
to business with the FIB. In the midst of their meeting, though, the IAA and Merryweather crash the party,. When things start looking
hairy for Michael, Trevor comes in to save the day, insisting that if anyone's gonna
kill Michael, it'll be him. Now that they've patched things up, they revisit their plans to
hit the Union Depository. Meanwhile, Franklin has been
dealing with his own problems. He eventually finds out
that his best friend Lamar has been set up by Stretch, an old friend who has been double-crossing them. With some help from the
always-bickering Trevor and Michael, he saves his best friend. Afterwards, Haines and
Norton warn Franklin that they'll call on him
to assassinate Trevor because he's liable to reveal that the FIB has been working with criminals. Michael and his son go
to his movie's premiere, and while on the red
carpet, Weston greets him with an ominous warning about his wife. He immediately ditches
the movie to return home, only to find Merryweather soldiers trying to kill his wife and daughter. He saves them and asks them to move out until things blow over. Finally, the Franklin,
Michael, and Trevor trio unite to pull off the big
Union Depository heist. After they successfully steal more money than they can imagine, Franklin gets a surprise visit from
none other than Weston. He wants Franklin to assassinate Michael, claiming that he inevitably
betrays everyone. Now, Franklin and you
have a decision to make. He can kill Trevor, kill Michael, or work with them and Lester
to kill all their enemies. If you choose to kill Trevor, Franklin will chase Trevor
down and burn him to death. Although Michael still considers
Franklin a close friend, taking out his old partner in crime doesn't really sit well with him. If you choose to kill Michael, Franklin will set up a meeting, but Michael catches on to
the situation and flees. After a chase, Franklin
catches up with him at a power station, and the two fight near the top of a tower. Finally, Franklin pushes Michael
off the edge, killing him. Franklin inevitably regrets his decision, and when Trevor finds out, he says he'll kill Franklin the
next time he sees him. Thankfully, the third
option is the canon ending. If you choose to work with Lester, they all come up with a plan that lures the FIB and Merryweather into a trap. Once they're all taken care
of, the main three split up to deal with each other's enemies, kind of a little switch-around of targets. Trevor kills Haines, who had
wanted to murder Michael. Franklin takes out the
leader of the Triads, who wanted Trevor dead, and
Michael hunts down Stretch, who had been messing
with Franklin and Lamar. Finally, Trevor ties up the last loose end by kidnapping Weston and putting
him in the trunk of a car. He calls Franklin and Michael,
and the three push him off a cliff, killing
Weston once and for all. And as the sun sets, the
three agree to be friends, flawed, awful, totally uncomfortable, and poorly mismatched friends. Man, I've never felt so many warm fuzzies about watching a man perish
in an exploding car before, but, hey, Grand Theft Auto
teaches you a lot about yourself. I wasn't kidding when we said there was a lot of murder and betrayal and revenge, but that's what makes these
stories worth telling. And there's a whole
other universe of stories worth telling in the 3D universe. We already covered that one,
so be sure to take a look. Also, subscribe to The Leaderboard because from indie to triple-A,
we love the games you play. I'm your host, Kyle,
and thanks for watching. (reflective synth music)


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Alberta Exotic Plants Wiki
Click here for Posey's Rogues: a gallery of exotic invasive and non-invasive plants of Alberta.
Learn where you can buy native seeds and plants – updated May 2018. (Download the Excel spreadsheet.)
Call for Volunteers – Board of Director's Positions
If you are passionate about native plants, ecosystems and their long-term sustainability and have experience coordinating people and projects, then the Alberta Native Plant Council (ANPC) Society's Board of Directors wants you!
We are looking to fill the following Board positions in March 2020:
– President (1 year term)
– Vice-president (1 year term)
– Secretary (2 year term)
For more information on these positions, see our "Volunteer" page or contact us at firstname.lastname@example.com
DEADLINE FOR RECEIPT OF INTEREST: JANUARY 31st 2020.
Registration is now open – please see our Workshop page here
Throughout the year, ANPC supports Plant Study Groups in four locations in the province. These meetings will run from October 2019 to May 2020. The discussion topics and dates are listed on our Plant Study Group page here.
The Alberta Illustrated Keys have been updated by Linda Kershaw and Lorna Allen. Species status now agrees with the most recent ACIMS listing (ACIMS, March 2018) and taxonomy agrees with the VasCan database of October 2018 (Brouillet et. al. 2018). Please check them out on the 2019 Keys to Alberta Species page. Four new families have been added January 8, 2020 (Apiaceae, Boraginaceae, Hydrophyllaceae and Onagraceae).
November 2019 Update : "The Rare Vascular Plants of Alberta book is in the process of being brought up to date with a second edition. See our "Rare Vascular Plants" page for the latest updates (November 2019). Photographs of many species are still required. The photograph wish list (revised November 26, 2019) and instructions for submission.
Growing Native Plants in Alberta – Guidance for communities, schools, homeowners, as well as reclamation and restoration resources click on our link here or access the source list above.
Please watch for ANPC's display tables, banners and pamphlets at professional, conservation and natural history, and community events around the province. Check out our "Outreach" page for events in the summer.
Our wonderful new handouts can be downloaded from our "Plant Publications" page.
ANPC, Box 52099 Garneau Postal Outlet, Edmonton AB T6G2T5 firstname.lastname@example.com All content copyright ANPC 2020
For any problems with this website, please contact the webmaster at: firstname.lastname@example.com

subset: commoncrawl


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ROCK ISLAND ARSENAL - We all know the I-74 Bridge is under construction. Now, the Iowa Department of Transportation is hosting a virtual reality experience.
You can explore the history of the Quad City area's bridges on the island. All visitors must get a pass that's good for a year. The museum just opened Tuesday, March 26. It's open until Saturday, June 1st, at the Rock Island Arsenal Museum.
DAVENPORT, IOWA - There's a brand-new event happening on Thursday, March 28th called "Women Fighting Hunger." It's hosted by the River Bend Foodbank, which is working to end childhood hunger in our area.
STERLING, ILLINOIS - Our own Meteorologist Andrew Stutzke will be participating in the Whiteside County Relay for Life Celebrity Pie Auction on Friday, April 5th. The auction will be broadcast LIVE on WSDR 1240AM beginning at 6 a.m. Bids on his pie creation and other items will benefit the American Cancer Society. Andrew will also be answering your weather questions live with an additional donation. To learn how to donate and for more information about the event, click here.


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MacProVideo – Dance Music Styles 111: Bass House [Tutorial, ENG]
Release Year : 2016 Manufacturer : MacProVideo Manufacturer Website : www.macprovideo.com/tutorial/dance-music-styles-111-bass-house Posted by : Adam Pollard Duration : 01:31:07 Distribution Type : Video Tutorial Language : English Description : How many dance music styles are there? Well, you'll find out because the multiplier will probably cover them all! Join Adam in this exploration of Bass House and… Read More »


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Within two months after Christ Church began, small groups were initiated by Pastors David and Marlinda Ireland to serve the personal needs of our members. Participating in small groups is one of the best ways to build friendships in a growing church. The focus of this ministry mirrors the overall mission of the church to "Unite people to God and people to people." The Biblical Pattern
The foundation of the small-group ministry is patterned after the New Testament model found in Acts 2:42, 46.
"They spent their time learning from the apostles, and they were like family to each other. They also broke bread and prayed together. Day after day they met together in the temple. They broke bread together in different homes and shared their food happily and freely." (CEV)
Similarly, our small groups afford you the opportunity to connect with other believers in a small, friendly environment. In the small-group format, members find a place where they can grow in their relationships with God and other members of the Christ Church family. It is in these small, intimate meetings that life-long friendships are established. The goal is to help each member grow in his or her fullest potential in Christ. Hundreds have commented that our small groups have provided a place for them to discover and grow in their spiritual gifts.
In a small group you will enjoy…
- Building new friendships with members of Christ Church
- Opportunities to share your life experiences
- Discovery and development of your spiritual gifts
- Questions and answers about spiritual matters
About Our Small Group Leaders
Our small group leaders are specially trained to guide each of their members toward fulfilling the Christ Church mission: "To unite people to God and people to people."
They are all members of Christ Church who have been selected for their strong passion and love for God and His people. Each takes seriously their role as facilitators trusted to provide a safe environment where healthy relationships can flourish.
As an extension of the pastoral ministry, our group leaders undergo an extensive curriculum of discipleship and pastoral care. When a need arises that is beyond the scope of their expertise, our leaders will refer the matter to one of the pastors.
What types of small groups are available?
At Christ Church we offer two types of small groups for you to choose from: LIFE Groups and Life Activity Groups.
Are Children Welcome at Small Group Meetings?
To get the most out of your small group experience, it is recommended that childcare arrangements are made for your children prior to the meetings. However, some groups do welcome children and are well equipped to accommodate them. Information regarding whether a particular group is equipped to accommodate children is available in the online small group directory at www.ChristChurchUSA.org.
Do I Have to Be a Member to Participate?
No, membership is not a requirement to participate in our Small Groups. Both Life Activity Groups and LIFE Groups are open to friends, family members and co-workers. Visitors are welcome to join us for a time of refreshments, Bible discussion and friendly conversation.
LIFE Groups are small groups consisting of 5–12 members that meet twice per month (typically the 2nd & 4th weekends of the month) in the leader's home or the home of a fellow Christ Church member.
"And let us consider how we may spur one another on toward on toward love and good deeds"
—Hebrews 10:24
What Takes Place at a LIFE Group Meeting?
Although the format of each group may vary depending on what is most suitable for its members, the basic elements of each meeting are as follows: The groups typically begin with worship and prayer followed by a Bible discussion that is intended to further complement the teaching at Christ Church's weekend services. The discussion outlines used by the LIFE Group Leader is used to facilitate group interaction and practical ways for people to apply the principles of Scripture to their daily lives. After praying with one another at the conclusion of the meeting, the group members enjoy light refreshments and casual conversation with one another.
"You know that I have not hesitated to preach anything that would be helpful to you but have taught you publicly and from house to house." —Acts 20:20


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Hosta Care
If you love hostas, you're in luck. They are among the toughest and easiest garden plants there are. Some will even grow in the cracks in the pavement. Given optimal care, the result can be simply breathtaking.
Still, there are some things you need to keep in mind if you want perfect, thriving hosta.
It will help if you've got a good insight in the hosta growing cycle, and when you're aware of the most important needs of your plants.
It would be easy if every cultivar required the same growing conditions, but, as you might have guessed, that's not the case. I'll try to give you some base rules, but you have to find out for yourself which conditions any given hosta prefers.
You'll find a lot of tips in this section about hosta propagation techniques.
A special place is reserved for the 'Hybridizing' topic. In my opinion, hosta hybridizing, the creation of new, (valuable) cultivars, takes a special place in the hosta hobby.
And there are those plants that will not perform well in the garden, no matter how much attention and care they get. These are useless as garden plants, but some of them have their merits in hybridizing. Most haven't. It's every hybridizer's obligation to evaluate every new cultivar before releasing it on the hosta market. Careful placement and a top quality soil will provide for optimal growing conditions. And, mind you, not every cultivar is a easy as that. Some will require some serious green thumbs to thrive.
And last, but unfortunately, not least, there are the diseases hostas can suffer from and the animals that can attack your plants.
If you have good advice on hosta care you'd like to share with your fellow hosta lovers, give me some feedback on this. I will try to spread the word through this site


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SATURDAY by jessica levick
April 15th 1989. One game of football is about to affect Liam and the city of Liverpool forever. SATURDAY is a fictional account of how the Hillsborough stadium disaster � which claimed the lives of 96 Liverpool supporters � unfolded for one family back home in Merseyside. Director/Writer: Mike Forshaw / Cast: Harrison Vaughan, Elizabeth Berrington, Neil Fitzmaurice World Premiere Sundance Film Festival 2015. Other Film Festivals & Awards: http://www.saturdayshortfilm.com/film-fe...als-awards/ Website: www.saturdayshortfilm.com Facebook: https://www.facebook.com/SaturdayShort/ Twitter: @saturdayshort
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A Tale of Winter Woe
Why you need to get your yard in shape as the summer wanes
With the fall season around the corner, it's easy to spend the remaining days of summer playing in the sun and stargazing on warm evenings. A healthy dose of fun should be a part of everyone's end of summer plans. But the end of summer means there's work to do if you are going to have an enjoyable, stress-free winter. I'll tell you a little story about how I learned this lesson the hard way, and then we'll get into a few steps you can take to avoid my fate.
Two years ago we lived on a steep hillside in the Oregon Cascades. A couple weeks after we moved in, my wife saw a small coyote working his way through our grassy backyard into the dense Douglas fir trees. As I mowed the lawn that early summer –– before the sun stripped the green from the blades of grass and left them brittle and brown –– I would look over my shoulder while I made my passes along the treeline. I wanted to see the coyote. I just didn't want to be surprised by him while the noise of the lawnmower drowned out all other sounds.
In the fall, my wife got pregnant and I got a full time job. At the same time, the leaves on the birch trees in our front yard and two large oaks out back went a stark shade of marigold, slouching toward sunflower. Large yellow leaves fell thickly on the burned out lawn. When the fall rains began, the blades of grass got their green flicker back. They grew in patches in the gaps between the leaves. My wife didn't have the heart to ask her exhausted husband to rake, and she couldn't do the work herself. I told her I'd get to it soon. I wanted to get the yard cleaned up before the first snows.
In early November I was outside with the rake and a large, holey tarp. I kept thinking I heard the coyote. There was a rustling up the hill above me where the grass disappeared into the woods. Rain began to fall. The leaves were already wet, so I pushed on.
I had dozens of small, heavy, wet piles around the yard when my wife pulled the back door open. "Can you come in here, quickly?" My face went red and I dropped the rake. I was running, but I didn't know it. "It's not me," she said, as if she could read my mind. "The toilet is flooding into the tub!"
There is little more challenging than finding a plumber on a rainy Sunday afternoon in rural Oregon. I had to try. Hours went by while we waited to pay double for a weekend visit. When he finally finished digging up the buried septic tank cap, the news was as bad as we had feared.
The next day it snowed. Those tiny piles of leaves were blown around the yard as the wispy first flakes landed. I was at work. The lawn would have to wait, even if it couldn't.
I didn't get to the yard after the first snow, or after the second. In the late winter our baby arrived. Small lumps of leaves still loosely piled lifted the blanket of snow into tiny mounds. Underneath the snow, the leaves rotted and killed the grass below them. The organic matter attracted grubs and bugs that in turn enticed moles from the riverbed a half mile down the hillside. By the time the snow melted, a maze of mole tunnels crisscrossed the yard. The leaves were soggy, heavy, black, brown.
I did get what was rakable off the ground by early April, but it was too late. The lawn was trashed with vermin, rotting leaves, and large muddy patches of nothing. At the end of April my wife was home alone when a bear lumbered through the backyard. The snow had pushed him down from the high country. The dog barked loudly against the glass of the sliding back door while the bear stopped on our patio to look over the yard. "Did it scare you?" I asked my wife that night. "I think our yard scared him," she said.
It happens to all of us. Life's little to-do lists pile up, and something has to give. Here are a couple ideas to get you out there and organized before this coming winter gets on top of you.
Start Early
This year, we're in a different house, but you can bet I spent last weekend trimming the trees and cutting the lawn down to make it easier to rake. When the leaves do fall, I'll be ready. I've put a large red X on the first two weekends in October. I'll do anything I can to get the yard in shape over those four days.
Stay Ahead
Finish anything you start. Unless your septic tank breaks, don't let anything pull you away from raking, hauling brush, and yard debris removal, until the job is complete. It will get you ahead, and then when the snow does fall, you can expend your energy keeping the driveway clear.
Help a Neighbor
Team up to help your neighborhood stay ahead of the seasons. Maybe you could cut your neighbor's lawn and your lawn in exchange for them taking care of the fall leaves. Or try agreeing to shovel a neighbor's driveway all winter if they will lay the weed repellent and grass seed in the spring.
Bonus Tip: Go Trashy will haul away your yard debris on-demand for an affordable, upfront price. More info below.
Go Trashy is on-demand junk removal
You snap a pic of the stuff you want gone
You get upfront prices from background-checked Providers
You select your Provider and your stuff is gone within hours


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Sack Bag Mockup is a professional mockup. It is available in hi-res PSD format with built-in smart object feature. You can use this to present your work.


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Honeywell's new stationary air conditioning refrigerant, Solstice N41, has received the official designation 'A1' for R466A by the American Society of Heating, Refrigerating and Air Conditioning Engineers (ASHRAE).
Solstice N41 is said to be the first A1 non-flammable, reduced global warming potential (GWP), R410A replacement refrigerant developed for stationary air conditioning systems and it will be commercially available in 2020.
George Koutsaftes, Honeywell Advanced Materials president, said: "We developed Solstice N41 to address the many regulatory and safety challenges facing the industry.
"This product is a non-flammable refrigerant developed as an R410A alternative and is also better for the environment, energy efficient, and cost-effective for the end user with minimal conversion required. Safety is paramount and the properties of Solstice N41 make it a groundbreaking technology for the industry."
Numerous regulations including Europe's F-Gas regulation and others progressing in developed countries require the phase down of high global-warming products, prompting the HVAC industry to seek new alternatives. The current industry standard refrigerant, R410A, must be replaced with an energy-efficient, lower-GWP solution with similar or better performance.
With a GWP that is 65 percent lower than R410A, Solstice N41 is said to delivers all three attributes.
Early testing has indicated that Solstice N41 requires minimal changes to equipment and no additional training for installation and repair technicians, allowing original equipment manufacturers to convert from R410A.
In addition, there is no additional safety or liability risk to homeowners and the whole supply chain. Solstice N41 is being extensively tested by over 15 OEMs and leading compressor manufacturers, having accumulated more than 30,000 hours of testing in different applications such as commercial AC (VRF, rooftops) and residential AC (unitary).
Industry has embraced the inaugural World Refrigeration Day (WRD) with events happening all over the world....
Victaulic plans to launch its new Victaulic StrengThin 100 Series E125 Installation-Ready butterfly valve, an ideal solution for HVAC applications....
Air Pro Ltd., an Authorized Dealer of Toshiba Air Conditioning, is a progressive HVAC, Sheet Metal and Refrigeration Company that is seeking an ambitious, hardworking and innovative HVAC SENIOR SERVICE TECHNICIAN to join the team in BERMUDA....
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It will come as no surprise to you all that I love the Plum Stitchery. If you've been reading along for a while, you may remember when I shared my interview with her. Her designs are so fun and so whimsical- they're hard to resist!
I've always loved this darling Atlas ornament. It's perfect for anyone who loves to travel. I also think it would be so precious framed in a nursery. I had mine finished with a gusset and a gold hook. The 3D effect will really make it stand out on our Christmas Tree this year. I'm so glad I finally found the time to get it stitched!
I think this project would be perfect for beginner to advanced stitchers alike because of the limited number of colors in the kit. It would make a great project to toss in your bag for summer travels for the same reasons, too! As always, the kit for this project is available from Needlepoint.Com as an online class. It is $90 and includes a hand painted canvas, thread pack, printed stitch guide and access to free online videos.
A limited number of kits are available and they've been flying out of the store!
Don't miss out- order yours online here.
PS. Don't miss out on 25% off. The May Kit Sale ends at Midnight on the 31st!


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Supporting the Most Exciting and Meaningful Missions in the WorldComputer System Analyst I
General: This position shall provide Technical and Information Assurance support for PMA-205 Tactical Training Range Systems through the Range Systems Branch of the Atlantic Test Range, Patuxent River, Maryland.
Education Required:
Clearance Requirements:
Work Conditions:
Conditions will vary. Most work is performed in enclosed buildings, but some outside work may be required. Work will include sitting, standing, walking, lifting, and reaching. Applicant should be capable of lifting 50-100 lbs. with the assistance of other employees.
PAE is proud to be an Equal Opportunity Employer. Our hiring practices provide equal opportunity for employment without regard to race, religion, color, sex, gender, national origin, age, United States military veteran's status, ancestry, sexual orientation, marital status, family structure, medical condition including genetic characteristics or information, veteran status, or mental or physical disability so long as the essential functions of the job can be performed with or without reasonable accommodation, or any other protected category under federal, state, or local law.


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If you are interested infolding golf umbrella,Xiamen Saint Sun Umbrella Co.,LTD has found 6 related results, so you can compare and shop! Try finding the one that is right for you by choosing the price range, brand, or specifications that meet your needs.
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Color Changing Umbrella, Rain Drop Colorful Straight Umbrella, Printed Change Color stick Umbrella When Wet


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One of the most common financial goals today has become financial freedom. This is especially true for the young generation, the millennials who wish to not only become rich but become independent to enjoy life. Financial freedom to them is all about freedom from the need to work to earn a livelihood. It is today easier for the younger generation to accumulate enough wealth. Many other individuals are also 'financially independent' today without even realising so. The question for these individuals really is what next? This article is about the post-financial freedom stage...
Financial Freedom: Congrats!
You are one of the very few, privileged individuals to be in such a financial position. You can be proud and happy about it. But there are also few other caveats you need to know or understand.
Its all about you & family: Do not wonder what your cousin or uncle or the person next door is thinking about you. You and your family should be comfortable with the important decisions taken and should support each other and happy to live a lifestyle you have planned to live.
Nothing is permanent: Remember, there have been many people who have been very rich and powerful in past but have lost it today. Nothing is permanent and there is no guarantee that you will be as financially strong as today forever. So be humble, grounded and careful in life.
You need to plan: Earning money is easy for most. However, financial freedom is not about earning money or how much money you have. One can be financially independent in very less finances than you. The real trick is in planning your finances post retirement such that you may continue to remain financially free. A backup plan, should you come in financial stress should also be planned.
Be Sure! The first and the last thing about financial freedom is to be sure that you can be so and also to ensure that nothing gets in between. The need for proper financial planning can never be overstated in such a scenario. You should also be sure that no possible event or scenario in future will be able to put you completely at risk.
What Next?
You need purpose: Being wealthy without any purpose or direction is not really desirable. You will be the same person but probably more lazy, less healthy /fit and even less happy than you were before. Remember, having a motivation, a passion, a direction and purpose is life is most important. Without it, you may feel a sense of emptiness with no agenda for the day /week.
Making decisions: Being in a privileged position, you would probably be able to take decisions more freely. Since money or wealth is not a big factor anymore, you need some basis for making your future decisions. Thus, your future decisions can be based on your personal values or in support of your purpose / passion in life or any other form of context. Deciding this context is important to give a sense of direction to your decisions.
Choosing a quality of life: Choosing the right quality of life is essential for you. You cannot say YES to everything. You also need to create a boundary for you and your family and say NO to things which can potentially negatively impact your status. Remember, some spending can be done when you can afford it but remember it adds value only to a point. Too much spending will only clutter your life, will not any value and negatively impact you.
Fix yourself first: The first and the best thing to do is to fix what is not good or broken in your life. Start with finances – clear all debt, ensure proper investments, ensure adequate insurance for all possible risks, ensure earmarked investments for all financial goals, ensure regular income flow. Once the finances piece is done, you can move on to other things in life like getting fit, living a healthy lifestyle, building networks, building social presence, giving more time to children, building strong relationships with your friends and family and so on.
Work after wealth: Many people will be happy to continue working in some form even if you don't really need to. Some people choose to be semi-retired where they may need to work but they would also be free to choose what kind of work they need to do and what portion of time they need to give. Working after wealth is a great idea as it will give purpose, make up for idle time, give income support, give additional financial comfort and surety while keeping you skilled and relevant in the industry.
Make world a better place: Making the world a better place is a great idea and you can lend your hand in many ways. Dedicating some portion of your time and/or money to some social cause can be a great way to towards satisfaction and happiness. You could also use your skills and knowledge to guide and help others who otherwise would not have access to same like for eg, legal, medical, financial or business advice, etc.
Make a list: The end point of what next question is preparing that one list you would ever need to make. This is a list that defines who you are and what you want from your life. So what can we include in this list? Here are a few things you can answer and keep updating this from time to time.
What are your most important goals?
How would you like spend the next 5 years?
What are the key values and principles you wish to live life with?
How would you like to see yourself in next 5 years?
What are the skills and things you want to be good at?
How would you live if you knew you are going to die in say next six months?
What is your mission, purpose in life?


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Search results for author:"Therese Laferriere"
Total records matched: 1 Search took: 0.048 secs
Integrating ICT into Higher Education: Investigating Onsite and Online Professors' Points of View
Gerard Fillion; Moez Limayem; Therese Laferriere; Robert Mantha
International Journal on E-Learning Vol. 8, No. 1 (January 2009) pp. 17–55
For the past two decades, information and communication technologies (ICT) have transformed the ways professors teach and students learn. The purpose of this study is to investigate the points of view of onsite professors (hybrid or blended mode)...
Topics: eLearning, Information Communication Technologies, Information Sciences, Post Secondary Education, Distance Education, Internet


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Maple identification blog post
Don Drife put together a great blog post over on his website about identifying different maple species found in Michigan. Check it out here: http://www.michigannatureguy.com/blog/2019/08/14/michigan-maples/ Don is also participating in the TASpeR project!
Sampling Kits Sent Out!
The TASpeR project is underway! We have sent out over 120 sampling kits to Citizen Scientist participants throughout the eastern United States. We are excited to start to get some leaf samples rolling into the lab.


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St George's Pre-School is based in St George's Church Hall, Wash Common, adjacent to Falkland Primary School.
We offer a fun and engaging curriculum in a large well resourced hall and outdoor play area. Our main aim is that children learn through exploration, play and have the skills to be independent in their learning.
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Why the government's pledge to end period poverty is so vitally important
As Philip Hammond vows to tackle period poverty in his spring statement, Laura Coryton, who has campaigned against tampon tax for five years, explains why this long-awaited action is so important.
In 2014, I started a petition against the 'luxury' tax rate we pay on period products. Before then, many (including me!) had never heard of the tampon tax. Yet for over 50 years we have been paying tax on period products, including tampons, sanitary pads and menstrual cups.
That's right: in 1973, a 17.5% tampon tax rate was introduced. Today, we still pay tax on period products, albeit at a reduced rate of 5% thanks to hero MP, Dawn Primarolo, who successfully campaigned to lessen the tax in 2001.
Perhaps the most astounding thing about the tampon tax is its context. Our tax system is riddled with absurdities. While period products are taxed because they are considered 'luxury goods', other items escape tax altogether because they are more 'essential'. These purchases include maintaining our private helicopters, eating exotic meats including crocodile and horse, and playing bingo, all of which enjoy a 0% tax rate. Now, I enjoy a good bingo game as much as the next woman, but surely we can all agree that period products are more 'essential' to our lives?
Five years ago, when I first discovered the sexism that riddles our tax system, I was confused. Then I got angry. Why were we all paying tax on period products when those who can afford private helicopters maintain them, tax free? I was gunning to sign a petition against the tax, having signed so many before, including Caroline Criado-Perez's petition to keep Jane Austen on our banknotes.
But I couldn't find any campaigns relating to tampon tax. So I decided to do something I had never done before: I started my own petition.
Since then, a lot has changed. My petition gained the support of over 320,090 amazing people and together we pushed our tampon-tax-ending legislation through Westminster and the EU. But now we're caught up in Brexit.
In 2016, David Cameron took our campaign to the European Union, which holds authority over all member states' taxation legislation, and proposed an unprecedented motion. He argued that for the first time in EU history, the Union should let any member state reduce a specific tax to their lowest national rate if they want to. That was tampon tax! His motion passed with unanimous support.
It will take until 2022 for this to finish going through European Parliament, but when it does, we expect to see waves of countries ending tampon tax. If we exit the European Union before 2022, then we have also secured UK legislation which is set to axe tampon tax 'as soon as legally possible'. So we either need to wait for Brexit, or 2022. That's a long time, but there is a lot of work to do in the meantime.
What was next for the fight against tampon tax?
Since my original petition, we have partnered with sister tampon tax petitions across the world. In 2015 we worked alongside Canadian activists, who spent six months planning the launch of their own tampon tax petition. When they launched it and presented their work to the Canadian Parliament, they won within just six months. That truly shows the power of online activism.
Over the past year, our sister petitions have won in places including India, Colombia and Canada. If you know anyone living in a country that has tampon tax, ask them to speak up! Even Barack Obama commented on the tax in 2016, arguing that if politics was a more female-friendly sphere then we wouldn't be dealing with gender discriminatory policies like the tampon tax. For context, campaigners across the USA are currently fighting against tampon tax, which is implemented to varying degrees and is being campaigned against on a state-by-state basis.
Now, we don't want the government to forget about their tampon tax promises, or the legislation that we have worked so hard to push through. So, to keep conversations about periods and tampon tax alive until 2022, and remind the government that we're keeping an eye on the situation, we have started to speak up about other issues affected by the period taboo.
The first was period poverty. In 2016, we launched our Homeless Period Project, which lobbied Bodyform and P&G, the creators of Tampax tampons and Always sanitary pads, to give their surplus stock to food banks and homeless shelters. After we successfully secured an agreed donation of 100,000 products, we started to wonder who else was effected by period poverty. That's when teachers started to speak up about their concerns over students facing period poverty.
Why was Philip Hammond's spring statement so important?
This issue eventually caught the attention of government ministers, as we have now seen. At midday today, the Chancellor of the Exchequer, Philip Hammond, announced his spring statement, the second most important financial statement of the year. Within this, he set his mini-budget for the year and revealed a whole heap of funding allocations, including new investments in digital technologies across the country and a £717m investment in new houses in areas including West London, Cheshire, Didcot, and Cambridge.
But one of the most amazing details in Hammond's statement, revealed at the end of his announcement, was his commitment to ending period poverty in our schools. He said that in response to "growing concern from head teachers over access to period products", he will fund the provision of free period products in secondary schools and colleges across England from next year. This is huge.
While five years ago I thought the tampon tax was an isolated problem, we have since discovered that many aspects of our everyday lives are dominated by the taboo surrounding periods, which has also led to period poverty and to girls missing school as a result. This announcement today is a huge step forward for schoolgirls, but also for anyone else who has ever campaigned against the period taboo and related topics including the tampon tax.
Hammond's announcement is also significant for the education of girls. Research by charity Freedom4Girls has shown that 10% of schoolgirls miss school because they do not have access to period products. That's more than 137,000 girls.
This can happen for many reasons. Research from the Social Metrics Commission has showed that 14 million people, including 4.5 million children, are living in poverty here in the UK. Food banks use in the UK has reached the highest on record.
What needs to change?
I regularly campaign with schoolgirls to secure the provision of period products in schools, and some say their families simply cannot afford to provide all of their children with sanitary towels. Many girls feel they don't want to take away from their struggling parents or ask them for period products when they can barely afford to put food on the table. I hear this story over and over again, and it's heartbreaking.
Simultaneously, research has recently shown that 91% of schools are facing funding cuts. None of the schools I've visited offer free period products in their bathrooms. One girl I met last weekend, while I was giving a school talk on campaigning, told me that her school has just five bathrooms in total. None of these have toilet paper, let alone period products. They have nowhere to turn and often feel that nobody is listening to them.
But today, at last, the government began to listen. Hammond's provision of free period products in schools and colleges across England will go a long way to ensuring girls simply have access to the vital period products they need to be able to go to school while on their periods. If girls can't access period products at home, they will be able to find the products they desperately need at their schools.
This period poverty announcement is particularly amazing because it was lobbied for through the internet, with mainly women and girls speaking up online. Projects like the Red Box Project, which has campaigned to provide free period products to everyone who needs them for years, and Amika George who started a petition calling on the government to give free period products to girls on free school meals, spread their messages and demands online. The government is listening to activism that springs across the internet.
When we campaign together on issues that have held us back for generations, just like period poverty, we can make huge changes. Together we have secured a change that a few years ago seemed inconceivable: a solution to period poverty here in the UK, period.
Images: Getty, Unsplash


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Recent Articles
- University of Washington and Nortis publish first organ-on-chip study modeling human liver-kidney interactions for preclinical pharmacology and toxicology studies
- Nortis at the 4th Drug Discovery Innovation Programme 2017
- University of Washington & Kidney Research Institute Kidney-on-a-chip will travel to International Space Station
- A pocket-size research revolution
- Nortis Awarded $688K for NIH Phase II SBIR Fast Track Grant in Human Blood Brain Barrier Model
August 2016
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July 2016
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February 2016
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Come visit the Nortis booth at the 2016 Molecular Medicine Tri-Conference, March 3rd – 11th 2016, and listen to a presentation by Senior Research Scientist, Dr. Henning Mann, to learn more about our commercial product.
A human liver microphysiology platform for investigating physiology, drug safety, and disease models
Posted by Matthew Hayes on
This paper describes the development and characterization of a microphysiology platform for drug safety and efficacy in liver models of disease that includes a human, 3D, microfluidic, four-cell, sequentially layered, self-assembly liver model (SQL-SAL); fluorescent protein biosensors for mechanistic readouts; as well as a microphysiology system database (MPS-Db) to manage, analyze, and model data. The goal of our approach is to create the simplest design in terms of cells, matrix materials, and microfluidic device parameters that will support a physiologically relevant liver model that is robust and reproducible for at least 28 days for stand-alone liver studies and microfluidic integration with other organs-on-chips. The current SQL-SAL uses primary human hepatocytes along with human endothelial (EA.hy926), immune (U937) and stellate (LX-2) cells in physiological ratios and is viable for at least 28 days under continuous flow. Approximately, 20% of primary hepatocytes and/or stellate cells contain fluorescent protein biosensors (called sentinel cells) to measure apoptosis, reactive oxygen species (ROS) and/or cell location by high content analysis (HCA). In addition, drugs, drug metabolites, albumin, urea and lactate dehydrogenase (LDH) are monitored in the efflux media. Exposure to 180 μM troglitazone or 210 μM nimesulide produced acute toxicity within 2–4 days, whereas 28 μM troglitazone produced a gradual and much delayed toxic response over 21 days, concordant with known mechanisms of toxicity, while 600 µM caffeine had no effect. Immune-mediated toxicity was demonstrated with trovafloxacin with lipopolysaccharide (LPS), but not levofloxacin with LPS. The SQL-SAL exhibited early fibrotic activation in response to 30 nM methotrexate, indicated by increased stellate cell migration, expression of alpha-smooth muscle actin and collagen, type 1, alpha 2. Data collected from the in vitromodel can be integrated into a database with access to related chemical, bioactivity, preclinical and clinical information uploaded from external databases for constructing predictive models.


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With 500 consecutive football games, Maryland fan goes deep with his pledge of allegiance
Maryland football fan Bob Baker has been going to games since he was an undergrad in the mid-1960s and hasn't missed a game since 1976.
Fabled 1963 Navy football team to be subject of documentary film by Michael Meredith
The Capital has learned that Navy's 1963 football team will be immortalized through a documentary film that is in the works.
The Sun Remembers: This Week in Sports History for Nov. 26-Dec. 2
Roger Staubach, Bert Jones and Bailey Howell are part of This Week in Sports for Nov. 26-Dec. 2.
After success at Western Michigan, P.J. Fleck trying to make Minnesota football 'elite'
P.J. Fleck has Gophers off to 3-0 start going into Saturday's game against Maryland
Catching up with ... former Maryland quarterback Mark Manges
As a junior, Manges led Maryland to 11 straight victories, a No. 4 ranking and a berth in the Cotton Bowl, earning a Sports Illustrated cover along the way.
Legendary Navy football coach Wayne Hardin dies at 91
College Football Hall of Famer and former Navy head coach Wayne Hardin has passed away at the age of 91. Hardin suffered a massive stroke on Tuesday and passed away this morning.
Navy football's new uniforms remind you which team the Mids want to beat
Navy football has its "Beat Army" slogan on new uniforms this weekend, just in case you needed a reminder.
Schmuck: Loss of Worth, Gulley could cost Navy against its biggest rival
Next week, the Mids will play Army West Point at M&T Bank Stadium and the conditions could not be better for the Black Knights to end their 14-game losing streak in the legendary rivalry.
Navy, Western Michigan could put bowl selections on hold
College notebook (Jan. 28): Loyola Maryland, Navy women's lacrosse picked to top Patriot League
The Greyhounds and Mids were picked to finish first and second, respectively, in a preseason poll of the league's head coaches and sports information directors.
New Maryland coach DJ Durkin won't coach Michigan in bowl game
New Maryland football coach DJ Durkin won't coach Michigan in its bowl game.
Sturdy on defense, woeful on offense, Maryland loses to No. 13 Michigan State, 24-7
Even with Michigan State's star quarterback out, Maryland (2-8, 0-6 Big Ten Conference) didn't pose much of an upset threat. The Terps turned the ball over five times.
Maryland expecting to face resilient Michigan State team this weekend
When Maryland travels to East Lansing, Mich., this weekend to face Michigan State, the Terps know they're going to face a Spartans squad in an interesting predicament. Late Saturday night, Michigan State fell to Nebraska on a controversial call on a late touchdown pass, where the officials elected not to call a penalty that would have wiped out the result. The Cornhuskers beat the Spartans, 39-38. The loss was Michigan State's first of the season and knocked them all the way from No. 6 to No. 14
As Big Ten football rises, Maryland and other programs try to keep pace
"You look at the discussions and the things that are being talked about nationally, the excitement, it's a 180-degree turn from the conversations that were going on a year ago," Penn State coach and former Maryland offensive coordinator James Franklin said Thursday.
Ohio State win caps impressive bowl season for Big Ten
The Big Ten deserves respect after Ohio State beat Oregon in Monday's college football playoff national championship game.
Edwin H. Brandt Jr.
Edwin H. Brandt Jr., a retired Baltimore Sun editor and author who led its sports department and covered classic 1950s Baltimore Orioles and Colts games, died of stroke complications.
50 years later, Army's upset win over Staubach-led Navy still resonates
Fifty years ago, Army defeated favored Navy, 11-8, behind Rollie Stichweh, a quarterback who outplayed his celebrated rival, Roger Staubach, that day.
Maryland to play Stanford in Foster Farms Bowl on Dec. 30
The Maryland football team learned Sunday that it will play Stanford in the Foster Farms Bowl on Dec. 30 at Levi¿s Stadium in Santa Clara, Calif.
Catching Up With ... former Colts punter Tom Gilburg
For five years Tom Gilburg punted for the Colts, averaging 41.4 yards a boot before quitting in 1966 with a bum knee. Three times, he finished among the NFL's top 10 punters while doubling as a second-string offensive tackle.
Long road to NFL for Arkansas' Kiero Small, a Cardinal Gibbons grad
Arkansas fullback Kiero Small has worked out for the San Diego Chargers and the Atlanta Falcons and visited the Cleveland Browns. Small also participated in the Ravens' local prospect day.
Source: Ravens met with draft prospect Michael Sam
Missouri defensive end Michael Sam conducted a formal meeting with the Ravens on Friday night at the NFL scouting combine, according to a league source.
Brendon Ayanbadejo calls Michael Sam a pioneer in tradition of Jackie Robinson
The significance of Missouri defensive end and NFL draft prospect Michael Sam's ground-breaking announcement that he's gay immediately struck a chord with former Ravens linebacker and special-teams ace Brendon Ayanbadejo.
The NFL and our sporting culture in general is willing to make excuses for moral failings in the face of talent, but somehow it's accepted that we overlook bravery in the face of bigotry.
Your guide to New Year's Day bowl watching
Army-Navy game in 1963 had a dream -- or nightmare -- ending
In 1963, Navy beat Army, 21-15, in a game deeply etched in the lore of their 144-year rivalry. The game was a legacy to President John F. Kennedy, who'd been assassinated in Dallas 15 days earlier.
The Sun Remembers: Oct. 27-Nov. 2
Joe Flacco, Sean Schaefer and George Franke are part of The Sun Remembers This Week in Sports for Oct. 27-Nov. 2
Navy overcomes slow start to beat Air Force, 28-10, bounce back from first loss
Playing a game that both teams were unsure would ever happen because of the U.S. government shutdown, the Midshipmen overcame a slow start offensively and a sloppy start defensively to beat Air Force 28-10, at Navy-Marine Corps Memorial Stadium.
Navy football overcomes slow start in 28-10 win over Air Force
Navy's 28-10 victory was its most one-sided since 1978 in what had become an annual down-to-the-wire series.
Government shutdown threatens cancellation of Navy-Air Force football game
The department of defense has ordered the stoppage of all service academy intercollegiate athletics as a result of the government shutdown, putting Saturday's Navy-Air Force football game in question.
The Sun Remembers: Sept. 29- Oct. 5
Roger Staubach, Jim Gentile and Buddy Young are part of The Sun Remembers This Week in Sports for Sept. 29-Oct. 5
The Sun Remembers: Sept. 15-21
Jamal Lewis, Eric Davis and Johnny Unitas are part of The Sun Remembers This Week in Sports for Sept. 15-21
The Sun remembers: Sept. 30-Oct. 6
Priest Holmes, Jim Palmer and Dick Nolan are part of The Sun Remembers This Week in Sports for September 30 to October 6
The Sun remembers: Sept. 9-15
Mark Manges, Tom Phoebus and Jack Fisher are part of The Sun Remembers This Week in Sports for September 9 to September 15
Sergio Kindle's course correction makes a big impact
In just his second full training camp, Kindle has plenty of ground to make up. But with each bull rush of an offensive lineman, each burst off the edge toward the quarterback, the outside linebacker gains some traction in his long road back.
Next Level Player of the Year: Tavon Austin, West Virginia
Maryland offensive coordinator Crowton might be fool's gold
Gary Crowton might be fool's gold. The new Maryland offensive boss comes in with a shiny resume. The man won a national title at LSU. He directed the offense for Oregon¿s 2005 squad that went 10-1 and won the Fiesta Bowl behind Kellen Clemens' savvy play. Simply put, he seems like a home run. Don¿t fall for it -- yet.
NFL combine: To run or not to run
Johnson, Utes earn sweet win
LSU routs Ga. Tech
A nice guy finishes first
Fantasy, reality and fish
Throwing a fit
Missouri's Pinkel gets raise, extension
Golden opportunity
Today's bowl games
Spare the result; reaching bowl is real victory for Terps
Bowl schedule
On values, 'Canes, not UNC, are real heels
Kornheiser shows colleagues at ESPN that he can take a hit


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Akesowan, A. (2015) Influence of konjac/secondary gum blend and sweetener on konjac gel. Pakistan Journal of Agricultural Sciences, 52 (4). pp. 1095-1099.
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This study aimed to investigate the effects of the proportion of konjac in a blend of konjac and secondary gum (κcarrageenan and xanthan) from 25:75 to 50:50 and the levels of sweeteners (sucrose at 220% (w/v) and erythritolsucralose at 0.22.5% (w/v)) on the firmness of konjac gels using response surface methodology. The variation in the proportion of konjac greatly affected the gel firmness with respect to the sweetener. Increasing the konjac proportion increased the firmness of the konjac gels that were cogelated with either of the secondary gums. At a high konjac proportion, an addition of 211% sucrose increased the firmness of the konjac/κcarrageenan gel but decreased that of the konjac/xanthan gel. The addition of erythritolsucralose had a small effect on the konjac gel firmness. Predicted models of konjac gels were reliable and can be used to optimize the conditions that provided the greatest firmness. The optimal conditions were found to use the konjac/secondary gum at approximately 50:50 with 17% sucrose or 2.5% erythritolsucralose for the konjac/κcarrageenan gel and with 2% sucrose or 0.76% erythritolsucralose for the konjac/xanthan gel.
|Item Type:
|Uncontrolled Keywords:
|Erythritolsucralose; Konjac gel formation; Response surface methodology; Sucrose; Xanthan; κcarrageenan
|Science and Technology > Food Science and Technology
|Research Center > Research Support Office
|Depositing User:
|Miss Niramol Sudkhanung
|Date Deposited:
|14 Jun 2016 10:06
|Last Modified:
|14 Jun 2016 10:06
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