text: mikerb22231 6 posts Posted June 16, 2018 Hi, my name is Mike. I'm a semi-serious comic fan with a small collection of graded comics and original art. I'd like to share a story of a dream commission and the many things I did to ruin it. I'll do my best to present the facts first and comment after. I welcome any of your comments or feedback. On March 15th, 2018 I paid for a commission through Felix Comic Art for a Daniel Warren Johnson piece. I've admired DWJ's art for about a two years, loved Extremity and was able to get a convention sketch from him at NYCC 2017. He is such a cool dude and his commissions have always impressed me so I thought I'd pull the trigger and get one with plans to frame it and display it proudly. His commission list was opened and closed on the same day, advertised for a C2E2 pick up. Ill be honest, financially I could not afford the $??? (Ill keep the number confidential) asking price. My job and personal financial situation is not what it once was, but I did it anyway. I decided to sell some old comics in order to offset the cost. I sold a large batch of old Spidey key issues that had just been sitting in a box, collecting dust and could use a better home. I had already paid for the commission but was able to sell just over 1k in comics to pay for the commission and have a bit extra for a rainy day. My initial idea was to just have Daniel draw a monster of his choice but then I thought how cool would it be to get a character I like fighting the monsters. Instead of a comic character I decided to request Harry Potter fighting a dragon. Cool idea, right? I email Felix requesting Daniel draw the scene from the Twi-Wizard Cup (Dragon, Harry, Golden Egg, Stadium with a crowd, maybe the Hogwarts castle in the background, etc). The commission was for one character and I was pushing it with making all these requests, and Felix responded saying just that. Since I had seen plenty of DWJ commissions with secondary characters, background, etc and I wanted to get my moneys worth I didn't think I was in the wrong to request particular details but I see now I might have been. After sharing a couple emails that same day I was told DWJ could do something similar to my request but that all the details might not be included. Ok, great! I'm getting an awesome Harry Potter commission from DWJ that I can't wait to frame! I stalked social media the weekend of C2E2 waiting to get a glimpse of my commission. I wasn't attending the show but since Felix allows you to do a mail order, and I assumed all the pieces would be completed prior to the show, I figured I'd be seeing it on Felix's Instagram account. I love watching the IG art shows from Felix during convention weekends and was super excited to see mine… but I didn't. In fact, weeks went by and I didn't see it. I debated messaging Daniel but part of me thought the longer I waiting maybe it would be a better commission. I debated messaging Felix but I knew he was taking a break from social media, email, conventions etc. Part of me also thought that if I messaged either Daniel or Felix my piece might get rushed and I didn't want that. Fast Forward to June 15th, 3 months after I had paid for my commission I thought I would be getting a week or two after C2E2 at the latest. I spoke with a couple friends of mine about the situation, told them I thought I had waiting to long and they agreed. They more than agreed, they actually fueled the fire a bit (something I regret I allowed to effect my thought process). So, I sat down to write an email to Felix or whoever it was that was doing Felix's emails since he had stepped aside for a bit. I was a bit of a jerk in the email, here are the highlights; I asked for a upgrade in art size and quality (11x17, cover quality) because of my wait time/bad experience and mentioned I had heard about horror stories of people not getting their commissions, some on his podcast, and was upset that it happening to me. I'll note that in the email I mistakenly said I'd waiting 4 months (March, April, May and June) but it had actually only been 3 (March 15th to June 15th). My mind set was this… and I'm being honest here, I wanted my commission. I was bummed it was taking so long and I thought there was a possibility I had slipped through the cracks and been forgotten. Whether there was a mistake or not I had thought I had waited too long and if I could guilt either Felix, his staff or Daniel into doing a bit extra because I had waited so long, even better. I typed the email and debated mutiltple times whether to send it. I was actually at a play and before it started I shared with the friend I was with the situation and they again fueled my fire a bit and told me to send it. So i did. 90 minuted later the play is at intermission and I have a missed call from Felix, a nicely written email response and a Paypal refund. In the voicemail Felix requested I call him. In the email Felix explained that they took as many commission as possible, maybe too many because DWJ was still working on them. He said that my art would be included with the next batch he got from Daniel and would be shipped right away. There was a 20 minute difference between when I received his email and when I got a paypal email informing me of my refund. I know now that Felix had time to think about my email and things I said and decided to refund me. I know this because we spoke about it. I mentioned I had a missed call from Felix. Well I called him back during intermission and from the start of the conversation I immediately realized just how much I F@cked up. I'll say this, I did not include my phone number on the email. Felix had it from a conversation we had more than a year prior. We had shared private messages on Instagram, Twitter and talked on the phone at length. He had given me his personal time many times before but I did not take the time to message him to check on my commission. I instead sent him an email being a jerk. Felix was pissed and was not in the mode to deal with me but he still gave me 15 minutes of his Friday night to talk to me and express just exactly how I was wrong. He expressed his frustration with my communication methods and said there was no way for him to read my mind and know that I was not happy. He also said that he felt that I was trying to extort a larger, cover quality piece. He explained that many times throughout the years when I had written him or messaged him he had always responded, including the email and call tonight. He challenged me on my "horror story" comment saying waiting a couple months is nothing, he has waiting 10 years before. He said, "We are done." in regards to this commission and that he really felt how I treated the situation was wrong. Lastly he challenged me to share this story on the CGC boards and see if I had waited too long, if he was in the wrong, etc. So here we are... but before you hit reply I want to be clear as to why I am sharing this story. I realized almost immediately when talking to Felix that I was 10,000% wrong. My mentality and approach to the situation, what I said in the email, so much of it was wrong. Sure, I paid for a commission that I expected to receive months ago but all I had to do was reach out for an update. I was acting cocky and entitled. I knew Felix was going through some stuff that had lead to him stepping aside from social media and, although I hope its not true, I think a very small part of me was trying to take advantage of that, which is so F%&$ck up. I'm so ashamed of how I acted, its out of character and not like me at all. Let this be a lesson. The customer is not always right. Do not think that you will always get your way. Acting immature and juvenile will get you no where. I was very wrong in my approach and thought that complaining would get me somewhere and instead it got me the exact opposite. I sold a bunch a key Spider-man comics to fund a commission from one of my favorite artist that I will not be getting now, and for good reason. I ruined a relationship with an art dealer who reps so many artists I respect and collect, hindering my chances of getting any more art from them. He will most likely share my email with DWJ and others, effecting my relationship with them. But the worst of it, I ruined a professional friendship with one of the best guys in the business. So to recap… instead of coming to the CGC boards to argue my side of things I am coming to CGC to offer a public apology. I was rude, unprofessional and childish and I'd like to apologize to Felix, Daniel and the whole Felix Comic Art team. Share this post Link to post Share on other sites


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Former Governor Ariyoshi receives one of Hawaii's highest honors
Former Governor George Ariyoshi received one of the state's highest honors Monday.
Former Governor George Ariyoshi received one of the state's highest honors Monday.
Lawmakers inducted Ariyoshi into the Aloha Order of Merit at the State Capitol.
It is an honor earned by providing extraordinary service to Hawaii while embodying the Aloha spirit.
Since its inception in 1993, just one other person has received the honor, the late Senator Daniel Akaka.
Ariyoshi was the first Hawaii-born Governor to be elected.
He served from 1973 through 1986, also making him the longest Governor to serve in Hawaii's history. He has also never lost an election.
"My ancestors may have created the concept of Aloha but among those who taught me what it actually meant is the person that we are honoring today," Governor John Waihe'e said.
Former Governor Waihe'e says it would've taken the entire afternoon to list all of Ariyoshi's accomplishments, among them pushing for sustainability.
Well before the word sustainability became a popular concept, Ariyoshi made sure more than a million trees were planted in Hawaii to restore our forests.


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Tag: perapera
Project Three: Promotional Poster
Project Three: Pera Pera App Final
Project Three: Screens & Userflow
Project Three: Application Features
Application Main Features/Navigation:
Circles: A feature that allows users to join groups according to their interests, school majors, languages, cultures, backgrounds ethnicity, etc.
Under Circles are the Discussions and Events features that give users multiple ways to communicate with other circle members either through their mobile devices, or physically through events and meet-ups.
Messaging: This feature allows for users to chat and directly message friends or language partners from around the world. The messaging feature allows for users to automatically and conveniently translate, correct, look-up, and define words and phrases in different languages without leaving the application.
Phrase Book: The phrase book allows for users to customize their language learning experience. All phrases, sentences, and words that the user favorites are recorded in the phrase book. The user can choose which phrases they want to save, practice, and use for reference for future conversations and interactions.
Settings: This feature includes features such as profile, profile settings, account settings, language settings etc. With a profile users can introduce themselves to other pera pera app users by including a short bio, and sharing their school, major, languages, and background.
Project Three: Research
For the research phase of project three we met together as a group and discussed different underserved demographics and groups of people that we could possible help with graphic design.
At first we had ideas such as garbage men, girls, children with disabilities, homeless people, and more. Our brainstorming eventually led to illegal immigrants, then immigrants (like me and my group member, Patricks' parents), and then eventually was narrowed down to international students who have difficulty understanding languages in the country they are studying in.
They are an underserved demographic because many people do not realize that international students, or students who recently immigrated to a new country have a lot of difficulties in their studies because they do not understand the language well. At the time I was researching international students, some of my friends near me (who are not international students, but moved to America a few years ago) was having a lot of troubles with her studies because she did not understand English well. Seeing her and other friends having difficulty with English and studying in America made me more passionate about finding a way to help the demographic.
After much thinking, we as a group, and with guidance from our professor, finally decided on making a mobile application that could connect international students with others while helping them learn and practice their languages. This application could have the potential of helping international students reach out, practice writing, speaking, and communicating in their languages, and provide them with a circle of friends/colleagues that they could approach for help.


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BMW G 310 GS Vs UM Motorcycles Renegade Classic comparison
Add bike to Compare
Compare BMW G 310 GS and UM Motorcycles Renegade Classic
Trying to figure out which of these bikes or scooters to buy? Compare the BMW G 310 GS Vs UM Motorcycles Renegade Classic on CarandBike to make an informed buying decision as to which bike to buy in 2020. This comparison has been carried out on the basis of prices, engine specifications, mileage, and features of these two-wheelers.
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The ex-showroom, New Delhi price of the UM Motorcycles Renegade Classic starts at ₹ 2.08 Lakh. On the other hand, the price of the base version of the BMW G 310 GS starts at ₹ 3.75 Lakh.
Compare Mileage
As for the claimed fuel efficiency, the UM Motorcycles Renegade Classic base engine returns 35 kmpl. The BMW G 310 GS base returns 30 kmpl.
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In the powertrain department, the UM Motorcycles Renegade Classic gets bhp Petrol engine, whereas the BMW G 310 GS comes in bhp Petrol engine.
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Many Fifers will be feeling the pinch after Christmas having spent money on food, drink and presents for loved ones.
But, for some, the festive period may have brought additional stress if they are already struggling with debt and money problems.
However, there are local organisations on hand to help anyone who might be worrying about their finances and how they are going to manage their bills after the festive season.
Christians Against Poverty (CAP) is one of a number of free debt counselling services available and the charity has a debt centre covering Burntisland and Kirkcaldy, which is supported by Burntisland Parish Church.
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Local CAP debt coaches and volunteers can visit clients in their own homes.
Doug Drysdale, CAP debt centre manager for Burntisland and Kirkcaldy, said home visits mean the free service can be accessed by anyone.
He said: "All our CAP Debt Centres are supported by local churches and our Kirkcaldy and Burntisland Debt Centre is supported by Burntisland Parish Church. We are able to provide a totally free service because CAP is a charity which receives donations from churches and individuals who want to help people.
"That means there are no financial barriers to receiving our help and we will work to provide a practical solution to your debts."
Doug said problem debt can affect anyone and the additional strain at Christmas can put a 'spotlight' on that debt.
He continued: "Many people hide their debt for years and don't seek help until their situation becomes critical with potential loss of home or tenancy or threat of court action.
"Changes in circumstances such as a relationship breakdown, becoming unemployed, physical or mental ill health and many other such issues can cause financial difficulties that can quickly spiral out of control.
"At CAP our trained debt counsellors will work out a realistic budget that prioritises essential bills. We will also negotiate affordable payments with each creditor and attempt to stop unfair interest and charges where possible."
Doug said that sadly, every year at CAP they are experiencing record numbers of referrals and Fife in 2019 was no exception.
"As an organisation we are continuing to rise to the challenge of providing more debt centres covering more areas," he said.
"Not only is there an increase in the number of referrals but also an increase in the complexities of debts and the devastating impact that debt can have on health and family life.
"Ten years ago, most problem debt came from credit cards, store cards and loans. Today, our nation is increasingly struggling with poverty debts like mortgages, rent arrears, utility bills, council tax arrears, complications over benefit payment, zero-hour contacts and more and more people are struggling to feed themselves or their family."
But Doug said trained debt counsellors can help people work out a realistic budget that prioritises essential bills: "In most cases a CAP Plan is set up with the client who will make one monthly payment into the CAP Plan to cover bills and debts and CAP will then distribute this on the client's behalf.
"People can use their CAP Plan to pay bills and debt repayments until they are debt free."
He added: "We understand that people can feel embarrassed or ashamed and it is a big step to ask for help but we are passionate about helping people find a real way out of debt. We are often asked whether our service is just for Christians. No, CAP will help anyone regardless of their religious beliefs. We monitor our services to ensure that everyone receives the same service regardless of race, nationality, religion, age, gender, marital status, sexual orientation, or disability."
He added: "Making the first call can be hard but the sooner you ring, the sooner you will have peace of mind. To book an appointment, please phone our new enquiries team, call free on 0800 328 0006."
Support from other organisations
Citizens and Rights Advice Fife (CARF) offer free, independent impartial and confidential information and advice on a wide range of subjects, including debt and money advice.
CARF's Money Advice Unit has a team of specialist money advisers.
CARF was the first organisation in Scotland to achieve accreditation of SNSIAP (Scottish National Standards for Information & Advice Provider's) in the areas of money advice and benefits.
There are a number of different ways that the Money Advice unit at CARF can help people with their financial issues.
Advisers won't tell people what to do, as they leave the choice up to them, but they will explain the options and the possible outcomes people might expect if they choose a particular course of action. They want to empower people to take control of their situation.
Advisers can assist with all sorts of financial issues, from budgeting to repossession.
They also take a holistic approach by reviewing debts, but also maximising income to make sure their clients are getting all the money they are entitled to as well as reviewing expenditure such as fuel/energy bills and insurance encouraging price comparisons in order to get the best deal.
As well as offering options for dealing with outstanding debt the team hope to be able to help their client's budget, plan and save for future expenditures.
The unit deals with over 2000 enquiries each year.
You don't have to be behind with payments to seek advice.
Jacqueline McDonald, CARF's debt advice co-ordinator, said that even if people are up to date but beginning to struggle it's never too early to seek advice. In fact, the earlier the better as the service is always a busy one.
It's often the case that just speaking to a member of the team can provide some relief from the pressure of dealing with financial difficulties.
Anyone looking for money advice can contact the CARF Money Advice Unit on 0345 1400 094 or WEBCHAT (via the website), from 8.30am to 4pm, Monday to Friday.
On contacting the CARF Money Advice Helpline, you will receive immediate information and advice and where appropriate, given details on how to obtain a face to face appointment with a Money Adviser.
Contact CARF via their website at www.cabfife.org.uk and follow them on Facebook and Twitter @cabfife.


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Have you ever felt like "something is wrong"?
If you answer yes it's because you have a human brain.
We all have this going on in our brains in the background to some extent. It stems from having a "survival brain" that had to be on the lookout for danger all the time in order to quite literally survive.
While this was super useful to get us to modern times, now it's getting in the way of our happiness, our goals, and quite honestly, our day to day life.
In this episode, I show you what to do when your brain defaults to "something is wrong" so you can use it to your advantage and stop letting it get in the way of you living your best life.
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Here are the best resources for this episode:
- When Your Brain Tells You Something Is Wrong (blog post)
- When It's Hard And Problems Are Forever (podcast)
- It's Supposed To Be Hard (blog post)
- Dream Year: How To Accomplish Your #1 Goal This Year And Feel Good Doing It (book + workbook)
- Personal Development Free Course (free training)
- Personal Development For Her (course)


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"When Rocky was diagnosed with brain damage, it must be noted that many athletes have a form of brain damage including football players, soccer players, and other individuals in contact sports such as rugby, etc. Rocky never went for a second opinion and yielded to his wife's wishes to stop.
So with the advent of new research techniques into brain damage, Rocky was found to be normal among fighters, and he was suffering the results of a severe concussion.
By today's standards Rocky Balboa would be given a clean bill of health for fighters."
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spending time in nature
AlpenGlow Lilac Garden
Green Style Catalog
Alpenglow Gardens began in 2003 as a gift of 15 plants from Reva Ballreich, a local horticulturist. Reva was a major grower and hybridizer of lilacs in the western United States. Like the Pied Piper, she led visitors through acres of blooming lilacs in her collection of 800 varieties. Reva's enthusiasm and wonder led Gary Parton down the lilac path and inspired him to create Alpenglow Gardens on his property at the base of Lily Rock.
Alpenglow Lilac Gardens opened in 2009 with over 275 lilacs planted in the one-acre garden that meanders up and down a gentle mountain slope amid towering pine trees. The 155 cultivars are from all over the world. The general collection features lilacs from 44 hybridizers. Alpenglow is divided into 11 smaller gardens honoring a specific theme or hybridizer. The gardens provide both an experience for the senses as well as a walk through the colorful history of this plant.
Come and see them for yourself this spring when the lilacs are in bloom and the gardens are open to the public. Call ahead to be sure the lilacs are ready (weather affects blooming, the timing of which varies from year to year), and that we aren't closed for a special event.
Copyright © 2015 All rights reserved.


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The ykzB and ykoL genes encode two peptides, of 51 and 60 amino acids, the functions of which are unknown. The ykzB and tnrA genes are contiguous and transcribed divergently. Expression of ykzB andykoL is induced by glutamate and is under the control of the TnrA global regulator of nitrogen utilization. TnrA regulated its own synthesis in glutamate minimal medium. Two DNA sequences (TnrAB1 and TnrAB2) homologous to the TnrA binding site are present in the region between tnrA and ykzB. Deletion mapping indicated that the TnrAB2 binding site was involved in activation of the ykzB promoter. In addition, transcription oftnrA depends on the presence of the TnrAB1 binding site. The ykzB and ykoL genes are probably in the same transcriptional unit. A single promoter involved in transcription in the presence of glutamate was mapped by primer extension.ykoL expression was induced by phosphate limitation and depended on the PhoP-PhoR two-component regulatory system. Its promoter was mapped to the region between ykoL and ykzB. Four boxes similar to the PhoP binding site are present upstream from the ykoL promoter. These boxes are probably recognized by PhoP∼P during the activation of transcription in phosphate limitation conditions.
In Bacillus subtilis, the expression of several genes, such as glnA (glutamine synthetase), ansA (asparaginase), ansB(aspartase), ureABC (urease), gabP(γ-aminobutyrate permease), nasABCDEF (nitrate assimilatory enzymes), and nrgAB (ammonium permease and nitrogen-regulated PII-like protein), that are involved in the transport and catabolism of nitrogen-containing compounds is controlled by nitrogen availability (2, 8, 9, 19, 20, 28). Three regulatory proteins, GlnR, TnrA, and CodY, and a sigma factor called SigL are involved in the control of nitrogen metabolism in response to nitrogen supply. GlnR and TnrA are involved in the regulation of genes with nitrogen source-dependent expression. GlnR and TnrA are very similar and belong to the MerR family of regulators. GlnR is a negative regulator of glnRA operon expression and represses several TnrA-regulated genes (24). It has been shown in vitro that GlnR binds simultaneously to two operators in the glnRApromoter region. TnrA activates transcription of the gabP,ureABC, nrgAB, and nas genes in response to nitrogen limitation. TnrA is also a negative regulator of the ammonium assimilatory enzymes, glutamine synthetase and glutamate synthase, during nitrogen-limited growth (29). The TnrA protein is active only during nitrogen limitation, whereas GlnR is active in conditions of nitrogen excess. All of the promoters positively regulated by TnrA contain a GlnR/TnrA binding site (TGT-NAN7-TNACA) located upstream from the −35 region (9). The CodY protein controls several genes in nitrogen metabolism, acetate metabolism, and competence. CodY repression occurs in cells growing in a medium rich in amino acids. CodY is thought to bind to a DNA structure containing AT-rich DNA sequences (25). The sigL gene in B. subtilisencodes a sigma factor equivalent to the sigma 54 of gram-negative bacteria (4). SigL is required for the transcription of several operons involved in the catabolism of arginine, ornithine (roc operons), isoleucine, and valine (bkdoperon) (5). Specific transcription factors controlling these operons have been identified (3, 5). However, there is no global nitrogen regulatory system equivalent to the NtrB/NtrC two-component system of gram-negative bacteria.
Under phosphate starvation conditions, several genes in B. subtilis, including phoA and phoB (alkaline phosphatases), pstS (inorganic phosphate transport system),tuaA (teichuronic acid biosynthesis), are activated by the two-component (PhoP-PhoR) regulatory system response regulator and histidine kinase. All pho-regulated promoters require a minimum of four consensus PhoP binding sequence repeats (TT(A/T/C)A(C/T)A) for activation (7). During the B. subtilis function analysis program, 1,200 mutants were constructed using the pMUTIN4 vector to study the function and regulation of the unknown "y" genes (27). This plasmid can be used to construct lacZ fusions. The capacity of various amino acids to act as nitrogen sources or inducers of gene expression and induction by phosphate starvation have been studied. Upon screening of this collection of mutants, we found that the expression of 30 y genes was increased by the presence of glutamate in the growth medium. In one of these mutant strains,lacZ expression was strongly induced by glutamate as well as by phosphate starvation. This strain contains pMUTIN4 inserted into theykoL gene. In this work, we describe the regulation of theykzB-ykoL operon and of the divergent gene tnrA. We found that this divergon was positively controlled by TnrA and by the two-component system, PhoP-PhoR.
Bacterial strains and growth conditions.The B. subtilis strains used in this work are described in Table1. Escherichia coli DH5α [supE44 Δlac U169 (φ80 lacZΔM15)hsdR17 secA1 endA1 gyrA96 thi-1 relA1] was grown at 37°C in Luria-Bertani (LB) medium and used for cloning experiments. B. subtilis was grown in LB medium and transformed with plasmid or chromosomal DNA as previously described (12). Transformants were selected on SP plates (4) supplemented with erythromycin (1 μg/ml), lincomycin (25 μg/ml), chloramphenicol (5 μg/ml), or kanamycin (5 μg/ml). B. subtilis 168 derivative strains were grown in minimal medium [11.4 mM K2SO4, 62 mM K2HPO4 · 5H2O, 44 mM KH2PO4, 3.4 mM sodium citrate · 7H2O, 0.8 mM MgSO4 · 7H2O, 0.4% glucose, 0.005% l-tryptophan, 4 mg of FeCl3/liter, 0.2 mg of MnSO4/liter, 5.5 mg of CaCl2/liter 1.7 mg of ZnCl2/liter, 0.43 mg of CuCl2 · 2H2O/liter, 0.6 mg of CaCl2 · 6H2O/liter 0.6 mg of Na2MoO4 · 2H2O/liter, 10 mM (NH4)2SO4 or potassium glutamate adjusted to pH 7 with 10 N NaOH] or in low-phosphate defined medium (LPDM) or high-phosphate defined medium (HPDM) (11), each supplemented with 50 μg of l-tryptophan/ml. B. subtilis tnrA mutants were constructed usingtnrA::Tn917 chromosomal DNA (strain SF706T provided by S. H. Fischer) transferred by transformation with selection for transposon-encoded erythromycin resistance. B. subtilis phoP-phoR mutants were constructed using chromosomal DNA from the strain MH5913 (provided by F. M. Hulett) and selected for tetracycline resistance.
DNA manipulations.Standard procedures were used to extract plasmids from E. coli (1, 22). Restriction enzymes were used as recommended by the manufacturers. DNA fragments were amplified by PCR (18, 21) using the Taq DNA polymerase (Stratagene). DNA sequences were determined by the dideoxy-chain termination method (23) using the modified T7 DNA polymerase (26) (Pharmacia).
β-Galactosidase assays.β-Galactosidase activity was estimated on plates by 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal) hydrolysis. β-Galactosidase specific activities were determined as described previously (16, 17) and expressed as nanomoles ofo-nitrophenol produced per minute per milligram of protein. Induction by glutamate was measured in samples taken in exponential growth phase, while induction by phosphate starvation was measured at the beginning of the stationary phase.
Plasmid constructs.pMUTIN4MCS (27) was used for gene disruption and for the construction of transcriptional fusions with the E. coli lacZ gene. The BFS1843 (ykoL) mutant was constructed in K. Devine's laboratory by insertion of pMUTIN4MCS between bases 1397629 and 1397747 of the B. subtilis genome (13). The ykoM′-lacZtranscriptional fusion was constructed using aHindIII/BamHI DNA fragment corresponding to the ykoM central region generated by PCR with oligonucleotides YKOMH (5′-GCCGAAGCTTCCGTGATAGCAAAGAGCACGGTTT-3′) and YKOMB (5′-CGCGGATCCGCTCCCTTACTAAAAACCCGTTTC-3′). This fragment was inserted between the HindIII andBamHI sites of pMUTIN4MCS, and the recombinant plasmid was used to transform B. subtilis 168. Integration of the construct into the ykoM gene was selected by erythromycin resistance, giving strain FA33.
The ykzB, ykoL, and tnrA promoter regions were obtained by PCR amplification using B. subtilischromosomal DNA as the template. The corresponding PCR-amplified DNA fragments were introduced into the EcoRI andBamHI sites of pAC5 (15) to createlacZ translational fusions (Fig.1). These constructs were used to transform B. subtilis. Recombinant strains were selected using chloramphenicol resistance. In these strains, the lacZfusions were integrated as single copies at the amyE locus. The synthetic primers used for PCR amplification were as follows: YKOL1 (5′-CGGAATTCCCTGATCTGTCTTACGG-3′) or YKOLE (5′-CGGAATTCGATCGGTTCAAAACGGAC-3′) and YKOL2 (5′-CGGGATCCCGAAGCTCAGAAATCGT-3′); YKZBE (5′-CGGAATTCGAATGATCTTCTGTGGTC-3′), YKZBE2 (5′-CGGAATTCCAAATAGAAGATTTTTGAAAAAATAC-3′), or YKZBE3 (5′-CGGAATTCATAATGCGTAACACCCAA-3′) and YKZBB (5′-CGGGATCCGCCAGTGAGACTGCTTT-3′); TNRAE (5′-CGGAATTCGCCAGTGAGACTGCTTT-3′), TNRAE2 (5′-CGGAATTCCAAAAATCTTCTATTTGATGTTAG-3′), or TNRAE3 (5′-CGGAATTCGCGTCAGGTTTTTTCTCT-3′) and TNRAB (5′-CGGGATCCGAATGATCTTCTGTGGTC-3′).
RNA extraction and primer extension.RNA extraction, primer extension, and oligonucleotide labeling reactions were performed as described previously (6). B. subtilis 168 was grown in minimal medium with ammonium sulfate or potassium glutamate as the sole nitrogen source to activate glutamate-inducible promoters and in LPDM or HPDM to activate phosphate starvation-inducible promoters. RNA was extracted, and primer extension was performed using the YKZBPE primer (5′-AACGTCTCCAGCATTTCATCACGC-3′) for theykzB transcript and the YKOLPE primer (5′-GGCGTCGTTCTTTTTCTAAAGCGG-3′) for the ykoLtranscript.
Characterization of glutamate-induced genes.Within the framework of the European systematic function analysis of B. subtilis genes, 1,200 mutants were constructed. Genes to be interrupted by the pMUTIN4MCS (27) vector were selected from the 3,000 unknown open reading frames identified during the completeB. subtilis genome sequencing project (13). The corresponding proteins show no significant similarities to known proteins in databases. The unknown genes were interrupted by single or double crossover, leading to (i) inactivation of the target gene and (ii) transcriptional fusions between the promoters and the reporter gene, lacZ. We were interested in nitrogen metabolism and amino acid utilization. To investigate the potential function of the unknown genes, we tested the growth of the various mutant strains on minimal medium plates supplemented with X-Gal and containing a single amino acid (proline, alanine, aspartate, glutamine, or glutamate) or ammonium sulfate as the sole nitrogen source. The induction or repression of target gene expression on the different plates was estimated by β-galactosidase activity. One of the mutants tested, BFA1843, which showed a strong activity on plates was chosen for further study. In this strain, the expression of the lacZfusion was strongly induced by glutamate, resulting in blue colonies on plates supplemented with this amino acid. β-Galactosidase specific activities were determined to be about 1 U/mg of protein in ammonium sulfate minimal medium and 2,100 U/mg in glutamate minimal medium. It was therefore 2,000 times higher with glutamate (10 mM) than with ammonium sulfate (10 mM) as the sole nitrogen source. The integration of pMUTIN4MCS into BFA1843 interrupts the ykoL gene. The results obtained show that ykoL expression is induced by glutamate.
Analysis of the ykoL promoter.To identify the promoter of the ykoL gene, PCR-amplified DNA fragments containing sequences located upstream from ykoL were fused to lacZ, creating translational fusions. These fusions were integrated into the B. subtilis chromosome at theamyE locus of strain 168. β-Galactosidase activity was determined in minimal medium containing 10 mM ammonium sulfate or 10 mM glutamate (Fig. 1A). The fusion present in strain FA5, extending from the tnrA NH2-terminal region to theykoL NH2-terminal region, gave a high level of β-galactosidase activity in glutamate medium. lacZexpression levels were 1,000 times higher with glutamate than with ammonia for this strain. The results also show that the ykoLpromoter is located between tnrA and ykoL. Strain FA20, which contains a lacZ fusion with a DNA fragment located between the ykzB COOH-terminal region and theykoL NH2-terminal region, expressed β-galactosidase at a low level in ammonium sulfate and glutamate minimal media. These results suggest that the glutamate-inducible promoter is not present upstream from the ykoL gene but probably resides in the tnrA-ykzB intergenic region. To test this hypothesis, strain FA21, containing a DNA fragment extending from the tnrA NH2-terminal region to theykzB NH2-terminal region, was constructed and integrated by a double crossover event at the amyE locus of strain 168. β-Galactosidase activity in FA21 cells grown in minimal medium containing 10 mM glutamate was 2,500 times higher than that in cells grown in ammonium sulfate. These results indicate that the glutamate-dependent promoter is located upstream from theykzB gene and that the ykzB and ykoLgenes probably form an operon.
Determination of the ykzB transcriptional start site.Primer extension experiments were performed to determine theykzB transcriptional initiation start site. Total RNA was extracted from strain 168 grown in minimal medium containing either 10 mM glutamate or 10 mM ammonium sulfate as the nitrogen source. The YKZBPE and YKOLPE primers, which are complementary to theykzB and ykoL coding sequences, respectively, were used for the extension reactions. One signal was detected using the YKZBPE primer and RNA extracted from a culture in glutamate medium, whereas no cDNA was detected with cells grown in ammonium sulfate (Fig.2). The transcription initiation start site was located 29 bp upstream from the translation initiation codon of ykzB. Examination of the upstream nucleotide sequence revealed a putative −10 region similar to the Pribnow box (Fig.3). However, the −35 region differs from the consensus sequence for ςA-dependent promoters. The same transcription initiation site was detected with the YKOLPE primer (data not shown). These results confirm that ykzB expression is induced by glutamate and that ykzB and ykoLare located in the same transcriptional unit.
ykoL expression is induced by phosphate starvation.During the systematic function analysis program, it was found that the ykoL gene is expressed under phosphate limitation conditions. We analyzed the expression of ykoL(FA20) in LPDM and in HPDM (Fig. 1A). As expected, β-galactosidase activity was 100 times higher in cells grown in low-phosphate medium than in cells grown in high-phosphate medium. In parallel, we measured the activity in strain FA21 in low- and high-phosphate media.ykzB was not expressed under phosphate limitation, suggesting the existence in the ykzB-ykoL operon of an internal promoter allowing ykoL expression during phosphate starvation.
Determination of the ykoL transcriptional start site under phosphate limitation conditions.To determine the transcriptional start site of the ykoL gene, RNA was extracted from strain 168 grown under low- or high-phosphate conditions. Primer extension experiments were performed using the YKOLPE primer. ykoL mRNA was absent in high-phosphate medium but was detectable in low-phosphate conditions (Fig. 2). The transcription initiation site was located 28 bp upstream from the translation initiation codon of ykoL. The nucleotide sequence of the region preceding position +1 contained a putative −10 region similar to the consensus sequence of ςA-dependent promoters. In contrast, the potential −35 region diverged greatly from the consensus sequence of the −35 region of promoters recognized by the ςA-containing RNA polymerase. These results confirm that ykoL has its own promoter, activated during phosphate starvation.
Regulation of ykoL expression by the two-component PhoP-PhoR system.As the PhoP-PhoR system activates the transcription of genes required for phosphate uptake and utilization during phosphate limitation, we expected that ykoL, which is induced in low-phosphate conditions, would be regulated by the PhoP-PhoR system. Evidence in favor of this hypothesis was provided by the presence of four putative PhoP binding sites TT(A/T/C)A(C/T)A with a spacing of 4 to 7 bp between repeats (Fig. 3) (7) and essential for promoter activity as demonstrated for the tuaA system (14). We tested whetherykoL was indeed regulated by the PhoP-PhoR system using strain FA20, in which the phoP-phoR genes were deleted. β-Galactosidase specific activity in the resulting FA26 strain was determined in cells grown in LPDM and HPDM (Fig. 1A). We found that the fusion was not induced by phosphate limitation in FA26, whereas it was induced in the parental strain FA20. β-Galactosidase activity in FA20 was 100 times higher in LPDM than in HPDM, whereas it was low in both media for the phoP-phoR mutant. Therefore, the expression ofykoL is controlled by the two-component PhoP-PhoR system in phosphate starvation conditions.
Regulation of ykzB and ykoL expression by TnrA.During nitrogen-limited growth in B. subtilis, TnrA induces the expression of several genes, includingureABC, nasA, and gabP. As TnrA is involved in the regulation of nitrogen metabolism genes, we thought it likely that the expression of ykzB would be regulated by this transcriptional factor. To test this, we introduced a null mutation of the tnrA structural gene into strain FA21. β-Galactosidase specific activity was measured in the resulting strain, FA24, grown in minimal medium with ammonia or glutamate (Fig.1A). Activity was much lower in the tnrA-defective strain grown in glutamate than in the wild-type strain (1,500 times higher), indicating that ykzB expression was positively regulated by TnrA. The tnrA mutation was also introduced by transformation into strain FA5, to give strain FA6. β-Galactosidase specific activity was assayed in cultures of FA6 in minimal medium containing 10 mM ammonia or 10 mM glutamate as the sole nitrogen source. The product of tnrA was also involved in the induction of ykoL expression (Fig. 1A). According to Wray et al. (30), all TnrA-regulated promoters contain a common inverted repeat sequence centered 49 to 51 bp upstream from the transcriptional start site. Two such putative TnrA sites, TnrAB1 and TnrAB2, were found centered 93 and 50 bp upstream from theykzB +1 position (Fig. 3). The first is identical to the proposed TnrA binding site, whereas the second has 15 of a possible 17 matches with the consensus sequence. The role of each site in the regulation of ykzB was then assessed. Expression of the fusion in the FA21 strain, covering both TnrA sites, was fully induced by glutamate. Using the same lacZ fusion, we sequentially deleted TnrAB1 and TnrAB2 sites to give strains FA30 and FA32, respectively. In strain FA30 (ΔtnrAB1), β-galactosidase specific activity was 3,000 times higher in cells grown in the presence of glutamate than in cells grown in ammonia (Fig. 1B). In contrast, β-galactosidase specific activity was very low in strain FA32 (ΔtnrAB1 ΔtnrAB2). These results indicate that the expression of ykzB is induced by the transcriptional nitrogen regulatory factor TnrA and that activation occurs through the TnrA site located 50 bp upstream from the transcriptional start site (TnrAB2).
Induction of ykzB and ykoL by glutamate in phosphate starvation conditions.In strain FA5, a high level oflacZ expression was observed in the presence of glutamate in both high- and low-phosphate conditions (Fig. 1A). In thephoPR strain FA18, lacZ expression was high in the presence of glutamate. As expected, this expression was completely abolished in the tnrA phoPR double mutant (FA19). In the FA20 strain containing the ykzB ykoL intergenic region,lacZ expression was induced in low-phosphate medium, confirming the existence of a PhoPR-dependent promoter. Conversely, in strain FA21, which contains the ykzB promoter,lacZ expression was induced only by glutamate.
Autoregulation of tnrA expression.It has been reported that TnrA positively regulates its own synthesis (9). We investigated the role of the two putative TnrA binding sites in the autoregulation of TnrA, by constructing atnrA′-′lacZ translational fusion using a DNA fragment extending from the ykzB NH2-terminal region to the tnrA NH2-terminal region. This fusion was integrated into the chromosome of strain 168 to give strain FA22 (Fig.1B). β-Galactosidase specific activity was measured in cultures of cells grown in minimal medium containing 10 mM ammonia or 10 mM glutamate as the sole nitrogen source. Expression of thetnrA′-′lacZ fusion was about 60 times stronger in the presence of glutamate than in the presence of ammonia. AtnrA::Tn917 null mutation was introduced by transformation into strain FA22, to give strain FA25. In this strain, β-galactosidase specific activity was very weak, confirming that TnrA is involved in the activation of its own expression. No effect of phosphate limitation was observed ontnrA expression.
To study the function of the two putative TnrA binding sites, TnrAB1 and TnrAB2, two DNA fragments, one containing one site (TnrAB1) and the other containing neither, were synthesized and used to construct two translational lacZ fusions, giving strains FA29 and FA31, respectively. β-Galactosidase specific activity was assayed in cultures of the FA29 and FA31 strains (Fig. 1B). In cultures of the FA29 strain, β-galactosidase specific activity was strong in minimal medium containing glutamate whereas in strain FA31 (ΔtnrAB1 ΔtnrAB2), it was as weak as that in ammonia, indicating that the TnrAB1 site is essential for tnrA expression. Thus, TnrA regulated the induction of its own expression through the specific TnrA binding site TnrAB1.
The ykzB and ykoL genes encode two peptides of 51 and 60 amino acids, respectively. This study shows that the expression of ykzB and ykoL is induced by glutamate. The expression of ykoL is also induced by phosphate limitation.
We demonstrated that the product of tnrA was involved in the activation of transcription in minimal medium containing glutamate. The induction by glutamate is rather unexpected because the glutamate pool is very large, up to 0.1 M, according to Hu et al. (10). TnrA activity may be modulated by a metabolite produced by the product of glnA, as previously suggested (29). Alternatively, the uptake of glutamate may be involved in the induction process. The effect of glutamate on the expression of tnrAor the target genes is also not known. In particular, we cannot exclude a direct interaction of glutamate with TnrA. Like other regulatory genes, tnrA expression is positively autoregulated, leading to a significant increase in the level of the corresponding protein during induction. This may partly account for the very high level of induction observed for ykzB. Indeed, ykzB is one of the mostly highly expressed of the 1,200 y genes ofB. subtilis studied during the European functional analysis program (unpublished results). The tnrA-regulated genes contain a common inverted repeat (TGT-NAN7-TNACA) located upstream from the transcriptional start site. This sequence has been shown to be the binding site for the TnrA protein (30). It has been shown that high-level activation of nrgABexpression occurs only if the TnrA site is centered 49 to 51 bp upstream from the transcriptional start site, indicating that the relative alignment of the −35 region of the promoter and the TnrA site is critical for activation (30). Two DNA sequences homologous to the TnrA binding site are present in the region betweentnrA and ykzB. Deletion mapping showed that the promoter proximal box (TnrAB2) was involved in the activation of theykzB promoter. The TnrAB2 site is centered 50 bp upstream from the transcriptional start site of the ykzB promoter, confirming that the distance between the putative TnrA binding site and the −35 sequence is crucial for promoter activation. The promoters of TnrA-regulated genes contain several mismatches within the −35 and −10 regions. We found that the −35 region of the ykzBpromoter differed from the consensus sequence for promoters transcribed by the ςA form of RNA polymerase. Although thetnrA promoter was not mapped by primer extension, a similar result was observed for expression of the tnrA gene itself. One putative TnrA binding site, TnrAB1, is necessary for induction of the expression of tnrA by glutamate. Removal of the upstream TnrA binding site led to a slight increase in tnrA andykzB expression. This may be due to the effects of competition between the proteins bound at the target sites.
As the expression of both ykzB and ykoL is induced by glutamate, these two genes probably belong to the same transcriptional unit. Moreover, only one promoter involved in transcription in the presence of glutamate was found by primer extension. The ykzB and ykoL genes are followed by ykoM on the chromosomal DNA of B. subtilis. However, we have shown that the expression of ykoM is not induced by glutamate and that this gene does not belong to the same transcriptional unit (data not shown). ykoL gene expression is also induced by phosphate starvation. Activation of ykoLtranscription depends on the PhoP-PhoR regulatory system. TheykoL promoter, which is activated by phosphate limitation, was mapped to the region between ykoL and ykzB. Four boxes with similarity to the PhoP binding site are present upstream from the ykoL promoter. These boxes are probably recognized by PhoP during the activation of transcription in phosphate limitation conditions. Previous work has shown that the predominant binding region in promoters controlled by PhoP is located at approximately the same position (i.e., 21 to 60 bp upstream from the transcription start site). The binding region contained multiple TT(A/T)ACA repeats separated by approximately 5 bp. At least four of these repeats were present in each promoter (7). In the ΔtrnA ΔphoPR double-mutant strain, ykoLwas not induced by glutamate or phosphate limitation, confirming that the expression of this gene is subject to double regulation.
The function of the YkzB and YkoL peptides is still unknown. However, we have shown that in a strain in which ykzB andykoL were deleted and in strains with deletions ofykzB alone or ykoL alone, there was no difference in the ykzB activation of transcription by thetnrA gene product in the presence of glutamate (data not shown). Therefore, the products of these two genes are not involved in induction of the operon in the presence of glutamate. The link between glutamate induction and regulation by phosphate limitation is difficult to interpret at the molecular level and needs further investigation.
We thank F. M. Hulett and S. H. Fisher for the gift of strains MH5913 and SF706T, respectively, and for helpful discussions. We also thank J. Bignon and S. Mazard for technical support, J. Knight for correcting the manuscript, and C. Dugast for secretarial assistance.
D. Robichon received a fellowship from the European Commission (contract BIO4-CT-95-0278). This work was supported by grants from the Institut Pasteur, Université Paris 7, and the Centre National de la Recherche Scientifique.
- Received 4 October 1999.
- Accepted 16 December 1999.
- Copyright © 2000 American Society for Microbiology


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"**!ATTENTION!** An active shooter has just been been reported in building #26 at Naval Medical Center San Diego. All occupants are advised to run, hide or fight. All non-emergency response personnel are asked to stay away from the compound, located at 34800 Bob Wilson Drive, San Diego, CA 92134," a post on the hospital's Facebook page read.
"For all concerned, the childcare facilities at NMC are currently secured. All children are accounted for and safe," officials announced in a statement shortly after 9:00 AM.
The Navy quickly had first responders and K9 units on the scene to clear the buildings, and people were ordered to shelter-in-place as the building went on lockdown so it could be cleared. A middle school and two high schools in the nearby area also went into lockdown.
By 10 AM, people were seen being lead out of the building with their hands up by law enforcement, though no arrests have been made.
Navy Capt. Curt Jones, commanding officer at the facility, said there was "nothing which substantiates" reports of a shooting.


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Walk into the artisan Peter Halley's sprawling West Chelsea flat and the aboriginal affair you'll apprehension is a massive adhesive casting replica of an age-old brownish carve of Poseidon, acquired from the Athens archaeological architecture a decade and a bisected ago. He — Poseidon, not Halley — stands nude and musclebound on a rolling platform, accoutrements ample like a surfer, aerial in advanced of a shelf of beaming paints. "I never meant to leave it here, but it's aloof so perfect," says the painter, gazing affectionately at the Greek god looming overhead. One time, a aloof European beneficiary visited and snarked that Cy Twombly kept absolute antiquities in his studio. The artisan chuckles. "I was like: 'I'm not Cy Twombly.' "
Halley, 64, a born-and-bred New Yorker, bobcat of the '80s East Village art scene, administrator of Index annual (now defunct), and aloft administrator of Yale's celebrated M.F.A. painting program, is continuing beside a archetypal of his newest project, an aggressive site-specific accession at Midtown Manhattan's Lever House. Bespectacled, fingers brindle with sherbet-colored paint, he's affable and impish, with a barren faculty of amusement and an abhorrence to self-glorification. He models himself afterwards Andy Warhol — "everything I apperceive about the architectonics of ability I got from him" — and subscribes to the Warholian bend of the artisan
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Boston Red Sox Captain Jason Varitek Honored at Fenway
He's part legend, part everyman and an all time pro. Jason Varitek was honored at Fenway Park today before the Red Sox played the Blue Jays. It was a great celebration for a man who was a true Red Sox, an everyday player and a leader. What was great was to see the Varitek family there—his older daughters, the baby, his wife and brothers.
He Owned Home Plate
Tek's wife, Catherine, Tweeted, "They may swing a better bat, They may have higher stats, but no one will ever be as fitting behind home plate as the Captain was! #ThanksTek"
How true that is. Number 33 owned home plate and so it was fitting that amongst the many gifts given to Jason by the Red Sox was the home plate from which he commanded his final home game for the Red Sox.
Although the Red Sox gave Tek a lot of gifts, including a red Ford F-150 truck, a set of golf clubs and two Fenway Park seats, I can't help but think the $25,000 donation to Children's Hospital meant the most to the guy. His kids are healthy, but his wife is a cancer survivor and he knows firsthand what a donation like that can mean. Plus, throughout his career he did outstanding charity work for the Red Sox.
Tek Honors Us
Sure he's the guy who got into Alex Rodriguez's face protecting his pitcher, blocked the plate without fear as runners came barreling down on him and stepped up when the team needed someone to take charge, but there's a side to Tek that is much more important.
It's his connection to his kids, his caring nature for others and his devotion to good deeds. I don't think this guy ever let his two championships and his status as one of the best catchers in the league ever go to his head.
Varitek said to the crowd yesterday, "I played here 15 years, and to come up with the words of what it has meant to me to play here and only here . . . I can't… How do you thank an entire nation for my entire career?"
Wow, he's thanking US for his career! That's what makes Varitek, Tek.
Wake and Tek
Of course, Tim Wakefield, another Boston institution who was honored earlier in the season, was there. It was great to see Varitek toss the first pitch of the game to Wakefield. It was a knuckler (of course) that bounced once (of course) before Wake caught it.
What can I say? It was a great ceremony to honor a great player and man.
I know what I can say. I miss those two guys so much. Wake and Tek were always willing to do whatever it took to help the team. True pros, true ball players and real people. We need more like them.


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Happy Place is an immersive, interactive pop-up exhibit with larger than life installations and multi-sensory rooms. Featuring 15,000 square feet of playful spaces and cheerful moments including a Super Boom room filled with 40,000 handmade gold flowers, an Upside Down roolm that defies gravity and bends into reality, the world's largest confetti dome, giant rainbow with a pot of happiness guests can jump and more!
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- Dates: August 23, 2019 - February 17, 2020
- Recurrence: Recurring daily
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West Bengal: A person held by BSF for smuggling 120 cattle to Bangladesh
In West Bengal's Kolkata city, a person was caught by the South Bengal Frontier Troops of Border Security Force (BSF) for allegedly smuggling 120 cattle to Bangladesh. As per media reports, the BSP troop also seized 12.25 lakh Bangladeshi currency called taka from his possession. PRO of BSF South Bengal, Kolkata on its Twitter handle, stated, "BSF troops of South Bengal Frontier during operations against trans-border crimes, affected seizure of 12.25 Lakh Bangladeshi currency, 120 cattle and apprehended 01 cattle smuggler on 31 Aug/01 Sept 2019, while these were being smuggled to Bangladesh". Src: https://bit.ly/2lnHuWz


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TEMPE, AZ, Jun. 6, 2019 — National Higher Education Day is designed to educate and inspire students. To celebrate, the Maricopa County Community College District (MCCCD) has introduced multiple initiatives and partnerships within Maricopa County. MCCCD has partnered with community and business organizations as part of its commitment to meet the growing demands for talent in industries like information technology, advanced manufacturing, allied health, construction, and financial services. These programs are designed to ensure MCCCD students have the skills and abilities required for success in Arizona's workforce. The District is providing innovative opportunities, both educational and institutional, from a cost-saving standpoint that includes an unprecedented reduction in tuition.
Recent highlights include:
- Mesa, Chandler-Gilbert and Scottsdale Community Colleges have partnered with East Valley cities, and technology giants Apple and Amazon Web Services, to launch the first-ever coding, cloud and networking certification, and degree opportunities through the creation of a Maricopa IT Institute at Mesa.
- Scottsdale Community College unveiled a state-of-the-art, expanded computer networking lab to help students become certified and trained in cloud computing and virtualization. SCC's lab and virtual data center's expanded capacity now supports 1,000 virtual machines.
- Nearly all Maricopa Community Colleges continue to work on expanding allied health opportunities to meet consistently growing community and health system needs, including a partnership with the Greater Phoenix Chamber of Commerce Foundation to establish incumbent worker upskilling certifications in high-demand specialty areas.
- For the first time this year, Glendale Community College participated in the National Cyber League competition and scored extraordinarily well. Students worked through real-world lab exercises and competed against over 3,000 other teams from across the United States. Glendale students placed at the Gold level and achieved the highest level of knowledge and skills (Top 15 percent) for the Western Conference.
"The success of our students is reflected in the health and economic vitality of our community, and sustained success in the future requires a long-term commitment to innovation. We are proud of the progress we have made in the past year to deliver on our promise of high quality, affordable education for almost 200,000 students at MCCCD – and there is more to come," said Dr. Maria Harper-Marinick, MCCCD Chancellor.
The Maricopa Community Colleges provide educational opportunities, to all who seek them, at an affordable price. MCCCD tuition and fees are just $85 per credit hour and on an average annual cost of $2,500 for full-time students. Graduating from a Maricopa Community College raises a student's lifetime earnings and helps them achieve their potential.
Join the Maricopa Community Colleges in celebrating our students for Higher Education Day, and help us advocate for higher education. #highereducationday
Questions about scholarships and financial aid opportunities? Call the Maricopa Financial Aid Answer Center at 1-888-994-4433.
Media Contact: Dasi Danzig, 480-268-6766, email@example.com
About The Maricopa Community Colleges
The Maricopa County Community College District includes ten regionally-accredited colleges – Chandler-Gilbert, Estrella Mountain, GateWay, Glendale, Mesa, Paradise Valley, Phoenix, Rio Salado, Scottsdale, and South Mountain – and the Maricopa Corporate College, serving more than 200,000 students with two-year degrees, certificates, and university transfer programs. MCCCD is ranked No. 19 on the 2018 Forbes list for Best Employers for Women.