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The elucidation of neoantigens is a critical step in predicting response to checkpoint blockade therapy and design of personalized cancer vaccines. We have developed an in silico sequence analysis method - pVACtools, to facilitate comprehensive neoantigen characterization. This modular workflow consists of tools for neoantigen prediction from somatic alterations (pVACseq and pVACfuse), prioritization and selection using a graphical web-based interface (pVACviz), and determining the optimal order of neoantigen candidates in a DNA vector-based vaccine(pVACvector).
Increasing interest in identifying the numbers and types of predicted neoantigens encoded by a cancer genome has placed an emphasis on the facility and precision of related computational prediction tools1. Several such efforts have been published2,3,4. Typically, these tools start with a list of somatic variants (in VCF or other formats), with annotated protein changes, and predict the strongest MHC binding peptides (8-11-mer for class I MHC and 13-25-mer for class II) using one or more prediction algorithms5,6,7. The predicted neoantigens are then filtered or ranked based on defined metrics including sequencing read coverage, variant allele fraction, gene expression, and differential binding compared to the wild type peptide (agretopicity index score8). However, of the small number of such prediction tools (Supp Table 1), most lack some key functionality, including predicting neoantigens from gene fusions, aiding optimized vaccine design for DNA cassette vaccines, and including nearby germline or somatic alterations into the candidate neoantigens9. An intuitive graphical user interface to visualize and efficiently select the most promising candidates is also critical to facilitate involvement of clinicians and other researchers in the process of neoantigen evaluation.
To address these limitations and to add facility for all end-users, we created a comprehensive and extensible framework for computational identification, selection, prioritization and visualization of neoantigens - 'pVACtools', that facilitates each of the major components of neoantigen identification. This computational framework can be used to identify neoantigens from a variety of somatic alterations, including gene fusions and insertion/deletion frameshift mutations, both of which potentially create very strong immunogenic neoantigens10. Further, pVACtools can facilitate both MHC class I and II predictions, and provides an interactive display of predicted neoantigens for review by the end user.
The pVACtools workflow (Fig 1) is divided into flexible components that can be run independently. The main tools in the workflow are: (a) pVACseq: a significantly enhanced and reengineered version of our previous pipeline11 for identifying and prioritizing neoantigens from a variety of tumor-specific alterations (b) pVACfuse: a tool for detecting neoantigens resulting from gene fusions (c) pVACviz: a graphical user interface web client for process management, visualization and selection of results from pVACseq (d) pVACvector: a tool for optimizing design of neoantigens and nucleotide spacers in a DNA vector that prevents high-affinity junctional epitopes, and (e) pVACapi: an OpenAPI HTTP REST interface to the pVACtools suite.
pVACseq11 has been completely implemented in Python3 and extended to include many new features since our initial report of its use. pVACseq no longer requires a custom input format for variants, and now uses a standard VCF file annotated with VEP12. In our own neoantigen identification pipeline, this VCF is the result of merging results from multiple somatic variant callers and RNA expression tools (Methods). Information that is not natively available in the VCF output from somatic variant callers (such as coverage and variant allele fractions for RNA and DNA, as well as gene and transcript expression values) now can be added to the VCF using vcf-annotation-tools (vatools.org), a suite of accessory scripts that we created to accompany pVACtools. pVACtools queries these features directly from the VCF, enabling prioritization and filtering of neoantigen candidates based on sequence coverage and expression information. In addition, pVACtools now makes use of phasing information provided in the VCF, taking into account all variants proximal to somatic variants of interest that alter neoantigen sequences9. Since proximal variants can change the neoantigenic peptide sequence and also affect neoantigen binding predictions, this is an important confounding factor to ensure that the selected neoantigens correctly represent the individual's genome. We have also expanded the supported mutation types for neoantigen predictions to include in-frame indels and frameshift mutations. These capabilities expand the potential number of targetable neoantigens several-fold in many tumors (Supplementary Data).
To prioritize neoantigens, pVACseq now offers support for as many as eight different MHC Class I epitope prediction algorithms and four MHC Class II prediction algorithms. The tool does this by leveraging the Immune Epitope Database (IEDB)13 and their suite of six different MHC class I prediction algorithms, as well as three MHC Class II algorithms (Methods). pVACseq supports local installation of these tools for power-users, or provides straightforward access by default via the IEDB RESTful web interface. In addition, pVACseq now contains an extensible framework for supporting new neoantigen prediction algorithms that has been used to add support for two new non-IEDB algorithms - MHCflurry14 and MHCnuggets15. By creating a framework that integrates many tools we allow for (a) a broader ensemble approach than IEDB, and (b) a system that other users can leverage to develop improved ensemble ranking, or to integrate proprietary or not-yet-public prediction software. Importantly, this framework enables non-informatics-savvy users to predict neoantigens from sequence variant data sets.
Once neoantigens have been predicted, the pVACseq ranking score is used to prioritize them. This score takes into account gene expression, sequence read coverage, binding affinity predictions, and agretopicity (Methods). In addition to applying strict binding affinity cutoffs, the pipeline also offers support for MHC allele-specific cutoffs16. Taking a step further than most commonly used approaches, we also offer cleavage position predictions via optional processing through NetChop17 as well as stability predictions made by NetMHCstab18.
Previous studies have shown that the novel protein sequences produced by gene fusions frequently produce neoantigen candidates19. pVACfuse provides support for predicting neoantigens from such gene fusions. Fusion variants may be imported in annotated BEDPE format from any fusion caller (we used INTEGRATE-Neo19). These variants are then assessed for presence of fusion neo-epitopes using predictions against any of the pVACseq-supported binding prediction algorithms.
Implementing cancer vaccines in a clinical setting requires multidisciplinary teams, many of whom may not be informatics savvy. To support this growing community of users, we developed pVACviz, which is a browser-based user interface that assists in launching, managing, reviewing, and visualizing the results of pVACtools processes. Instead of interacting with the tools via terminal/shell commands, the pVACviz client provides a modern web-based user experience. Users complete a pVACseq process setup form that provides helpful documentation and suggests valid values for inputs. The client also provides views showing ongoing processes, their logs, and interim data files to aid in managing and troubleshooting. After a process has completed, users may examine the results as a filtered data table, or as a scatterplot visualization - allowing them to curate results and save them as a CSV file for further analysis.
Furthermore, to support informatics groups that want to incorporate or build upon the pVACtools features, we developed pVACapi, which provides a HTTP REST interface to the pVACtools suite. Currently, it provides the API that pVACviz uses to interact with the pVACtools suite. Advanced users could develop their own user interfaces, or use the API to control multiple pVACtools installations remotely over an HTTP network.
Once a list of neoantigen candidates has been prioritized and selected, the pVACvector utility can be used to aid in the construction of DNA-based cancer vaccines. The input is either the output file from pVACseq or a fasta file containing peptide sequences, and pVACvector returns a neoantigen sequence ordering that minimizes the effects of junctional epitopes (which may create novel antigens) between the sequences. This is accomplished by using the core pVACseq services to predict the binding scores for each junctional peptide and by testing junctions with spacer20 amino acid sequences that may help to reduce reactivity. The final vaccine ordering is achieved through a simulated annealing procedure that returns a near-optimal solution, when one exists.
pVACtools has been used to predict and prioritize neoepitopes for several neoantigen studies21–23 and cancer vaccine clinical trials (e.g. NCT02348320 and NCT03122106). We also have a large external user community (the original 'pvacseq' package has been downloaded over 37,000 times from PyPi, and the 'pvactools' package has been downloaded over 9,000 times) that has been actively evaluating and using these packages for their neoantigen analysis, and has also helped in the subsequent refinement of pVACtools through feedback.
To demonstrate the utility and performance of the pVACtools package, we downloaded exome sequencing and RNA-Seq data from The Cancer Genome Atlas (TCGA)24 from 100 cases each of melanoma, hepatocellular carcinoma and lung squamous cell carcinoma, and used patient-specific MHC Class I alleles (Supp Fig 1) to determine neoantigen candidates for each cancer. By extending support for additional variant types as well as prediction algorithms, we produced 42% more neoantigens versus the previous version of pVACseq11. (Supplementary Data)
As reported from our demonstration analysis, a typical tumor has too many possible neoantigen candidates to be practical for a vaccine. There is therefore a critical need for a tool that takes in the input from a standard sequencing analysis pipeline and reports a filtered and prioritized list of neoantigens. pVACtools enables a streamlined, accurate and user-friendly analysis of neoantigenic peptides from NGS cancer datasets. This suite offers a complete and easily configurable end-to-end analysis, starting from somatic variants and gene fusions (pVACseq and pVACfuse respectively), through filtering, prioritization, and visualization of candidates (pVACviz), and determining the best arrangement of candidates for a DNA vector vaccine (pVACvector). Furthermore, by supporting additional classes of variants as well as gene fusions, we offer an increase in the number of predicted epitopes which is even more important in the case of low mutational burden tumors. Finally, by extending support for multiple binding prediction algorithms, we allow for a consensus approach. The need for this integrated approach is made abundantly clear by the high disagreement between these algorithms observed in our demonstration analyses (Supplementary Data).
The results from pVACtools analyses are already being used in cancer immunology studies, including studying the relationship between tumor mutation burden and neoantigen load to predict response in checkpoint blockade therapy trials and the design of cancer vaccines in ongoing clinical trials. We anticipate that pVACtools will make such analyses more robust, reproducible, and facile as these efforts continue.
Additional information
Online Methods: TCGA data pre-processing
Aligned tumor and normal BAMs from BWA25 (version 0.7.12-r1039) as well as somatic variant calls from VarScan226,27 (in VCF format) were downloaded from the Genomic Data Commons (GDC, https://gdc.cancer.gov/). Since the GDC does not provide germline variant calls for TCGA data, we used GATK's28 HaplotypeCaller to perform germline variant calling using default parameters. These calls were refined using VariantRecalibrator in accordance with GATK Best Practices29. Somatic and germline missense variant calls from each sample were then combined using GATK's CombineVariants, and the variants were subsequently phased using GATK's ReadBackedPhasing algorithm.
Phased Somatic VCF files were annotated with RNA depth and expression information using VCF annotation tools (vatools.org). We restricted our analysis to only consider 'PASS' variants in these VCFs as these are higher confidence than the raw set, and the variants were annotated using the "--pick" option in VEP.
Existing in silico HLA typing information was obtained from The Cancer Immunome Atlas (TCIA) database30.
Neoantigen prediction
The VEP-annotated VCF files were then run through pVACseq using all eight Class I prediction algorithms and for epitope lengths 8-11. The current MHC Class I algorithms supported by pVACseq are NetMHCpan31, NetMHC6,31, NetMHCcons32, PickPocket33, SMM34, SMMPMBEC35, MHCflurry14 and MHCnuggets15. The four MHC Class II algorithms that are supported are NetMHCIIpan, SMMalign, NNalign, and MHCnuggets. For the demonstration analysis, we limited our prediction to only MHC Class I alleles due to availability of HLA typing information from TCIA, though predictions of Class II can be just as easily generated using pVACtools.
Ranking of Neoantigens
To help prioritize neoantigens, a ranking score is assigned where each of the following four criteria are assigned a rank-ordered value (where the worst = 1):
B = binding affinity
F = Fold Change between MT and WT alleles
M = mutant allele expression, calculated as (Gene expression * Mutant allele RNA Variant allele fraction)
D = DNA Variant allele fraction
A final ranking is based on a score obtained from combining these values: Priority Score = B+F+(M*2)+(D/2). This score is not meant to be the final word on peptide suitability for vaccines, but was designed to be a useful metric.
Pipeline for creation of pVACtools input files
pVACtools is designed to support a standard VCF variant file format and thus, should be compatible with many existing variant calling pipelines. However, as a reference, we provide the following description of our current somatic and expression analysis pipeline (manuscript in preparation) which has been implemented using docker, CWL36, and Cromwell37. The pipeline consists of workflows for alignment of exome/DNA- and RNA-Seq data, somatic and germline variant detection, RNA-Seq expression estimation as well as optional HLA typing.
This pipeline starts with raw patient tumor exome or cDNA capture38 and RNA-seq data and produces annotated VCFs for neoantigen identification and prioritization with pVACtools. Our pipeline consists of three main components: DNA alignment, variant detection and annotation, as well as RNA-seq data processing. More specifically, we use BWA-MEM25 for aligning the patient's tumor and normal exome data. The output BAM then undergoes merging (Samtools39 Merge), query name sort (Picard SortSam), duplicate marking (Picard MarkDuplicates), position sorting followed by base quality recalibration (GATK BaseRecalibrator). GATK's HaplotypeCaller28 is used for germline variant calling and the output variants are annotated using VEP12 and filtered for coding sequence variants.
For somatic variant calling, our pipeline combines the output of four variant detection algorithms-Mutect240, Strelka41, Varscan26,27 and Pindel42. The combined variants are normalized using GATK's LeftAlignAndTrimVariants where the indels are left-aligned and common bases are trimmed. Vt43 is used to split multi-allelic variants. Several filters such as gnomAD allele frequency, percentage of mapq0 reads, as well as pass-only variants are applied prior to annotation of the VCF using VEP. We use a combination of custom and standard plugins for VEP annotation (parameters: --format VCF --plugin Downstream --plugin Wildtype --symbol --term SO --transcript_version --tsl -- coding_only --flag_pick --hgvs). Variant coverage is assessed using bam-readcount (https://github.com/genome/bam-readcount) for both the tumor and normal DNA exome data and is also annotated into the VCF output using VCF-annotation-tools(vatools.org).
Our pipeline also generates a phased-VCF file by combining both the somatic and germline variants and running the sorted combined variants through GATK ReadBackedPhasing.
For RNA-seq data, the pipeline first trims the adapter sequence using flexbar44 and aligns the patient's tumor RNA-seq data using HISAT245. Two different methods, Stringtie46 and Kallisto47, are employed for evaluating both the transcript and gene expression values. Additionally, coverage support for variants in RNA-seq data can also be assessed through bam-readcount. This information is added to the VCF using VCF-annotation-tools and serves as an input for neoantigen prioritization using pVACtools.
Optionally, our pipeline can also run HLA-typing in silico using OptiType48 when clinical HLA typing is not available.
pVACtools is written in Python3. The individual tools are implemented as separate command line entry points that can be run using the 'pvacseq', 'pvacfuse', 'pvacvector', 'pvacapi', and 'pvacviz' commands to run the respective tool. pVACapi is required to run pVACviz so both the 'pvacapi' and 'pvacviz' command need to be executed in separate terminals. For pVACseq, the PyVCF package is used for parsing the input VCF files. The mhcflurry and mhcnuggets packages are used to run the MHCflurry and MHCnuggets prediction algorithms, respectively. The pandas package is used for data management while filtering and ranking the neoantigen candidates in pVACseq and pVACfuse. The simanneal package is used for the simulated annealing procedure when running pVACvector. pVACapi is implemented using Flask and Bokeh. The pVACviz client is written in TypeScript using the Angular web application framework, the Clarity UI component library, and the ngrx library for managing application state. The test suite is implemented using the Python unittest framework and GitHub integration tests are run using travis-ci (travis-ci.org). Code changes are integrated using GitHub pull requests (https://github.com/griffithlab/pVACtools/pulls). Feature additions, user requests, and bug reports are managed using the GitHub issue tracking (https://github.com/griffithlab/pVACtools/issues). User documentation is written using the reStructuredText markup language and the Sphinx documentation framework (sphinx-doc.org). Documentation is hosted on Read The Docs (readthedocs.org).
Data availability
Data from 100 cases each of melanoma, hepatocellular carcinoma and lung squamous cell carcinoma were obtained from TCGA and downloaded via the Genomics Data Commons (GDC). This data can be accessed under dbGaP study accession phs000178. Data for demonstration and analysis of fusion neoantigens was downloaded from the Github repo for Integrate (https://github.com/ChrisMaherLab/INTEGRATE-Vis/tree/master/example).
Software availability
The pVACtools codebase is hosted publicly on GitHub at https://github.com/griffithlab/pVACtools and https://github.com/griffithlab/BGA-interface-projects (pVACviz). User documentation is available at pvactools.org. This project is licensed under the Non-Profit Open Software License version 3.0 (NPOSL-3.0, https://opensource.org/licenses/NPOSL-3.0). pVACtools has been packaged and uploaded to PyPi under the "pvactools" package name and can be installed on Linux systems by running the 'pip install pvactools[API]' command. Installation requires a Python 3.5 environment which can be emulated by using Conda. Versioned Docker images are available on DockerHub (https://hub.docker.com/r/griffithlab/pvactools/).
Author contributions
JH and SK were involved in all aspects of this study including designing and developing the methodology, and writing the manuscript, with help from CAM, ERM, OLG and MG. JH analyzed and interpreted data with input from HX, CJL, SZ and Y-YF. SK wrote the software code with help from ATW, APG, AZW, MN and JW. JM developed pVACviz and pVACapi with assistance from APG. JN, MN, and CAM developed pVACvector. WEG provided input about clinical needs for vaccine design to help improve the tool features. OG and MG supervised the project and revised the paper. All authors read and approved the final manuscript.
Competing interests
The authors declare no competing interests.
We thank the patients and their families for donation of their samples and participation in clinical trials. We also thank our growing user community for testing the software and providing useful input, critical bug reports as well as suggestions for improvement and new features. We are grateful to Drs. Robert Schreiber, Gavin Dunn and Beatriz Carreno for their expertise and guidance on foundational work on cancer immunology using neoantigens and suggestions on improving the pipeline.


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GBPCAD has been on a downtrend since the beginning of May, sliding to a two-year low of 1.5874 on Friday. The downside pressure has eased somewhat this week with momentum indicators pointing to a weak positive movement. The RSI is rising but has some way to go before escaping out the bearish territory above 50. The stochastic oscillator is also signalling an improved bias in the short term, with the %K line crossing above the %D line, though they both remain close to oversold territory.
For the pair to gain further positive traction, prices would need to first recover above the 20-day moving average in the 1.6140 regions, which is not too far from the early July swing low of 1.6144. A successful climb above this zone would shift the near-term bearish bias to a bullish one. This would then bring into focus three Fibonacci retracement levels of the upleg from 1.6144 to 1.6440: the 78.6% Fibo at 1.6208, the 61.8% Fibo at 1.6257 and the 50% Fibo at 1.6292.
A break above the 50% Fibonacci would clear the way for the 50-day moving average (currently at 1.6423) and this could mark the start of a switch in the medium-term outlook from bearish to neutral.
However, if the positive momentum fizzles out, prices are likely to seek support initially at the 200% Fibonacci extension at 1.5848. A drop below this level would reinforce the bearish picture and the bears would next target the 238.2% Fibonacci at 1.5735 and the 261.8% Fibonacci at 1.5665.

subset: commoncrawl


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FREE STANDARD SHIPPING ON QUALIFYING ORDERS OVER $82.00 - Offer valid at kitterytradingpost.com. Tax and shipping not include in qualifying amount. Offer is for standard ground shipping only to United States excluding Alaska and Hawaii. Offer valid on Alpine Skis, all other additional/oversized freight charges still apply. Offer not valid on firearm purchases. Offer not valid on retail store purchases, previous purchases or special orders. Offer limited to stock on hand, and is not redeemable for cash. KTP reserves the right to exclude certain products from this promotion. Offer subject to change without notice.
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@Tuinslang the main reason is that, like everyone else playing this game, I'm here as an escape from work, reality, friends, etc. I wanted to create things, because I enjoy creating things. I enjoy programming, I enjoy UI. I also thought people might enjoy the things I create. I did not expect that to come with the weight of dealing with people being rude. It's obvious now to me how foolish that was to ignore, but I hadn't considered it. If you used my work, I'm sure you noticed that I have no problem fixing bugs, I pride myself on reacting quickly to bugs. Reporting problems was never an issue, constant requests for things I'd explicitly said I wouldn't do were. Somewhere along the line people got it in their head that their criticism and feature requests were valuable given their use of my work.
@Pawkette, seriously, you shouldn't give up just for a bad (or bad ones) comment. Do you really think the bad ones overcome the good ones? Don't you feel all these people support?
If someone bothers you, ignore them. I used to get very sad or dissapointed with things like that, but now, at my age, I just ignore people and enjoy the support of the ones who deserve my happiness
She explained the reason here: http://www.reddit.com/r/elderscrollsonline/comments/22ewdz/which_addons_do_you_have_installed/cgmcv03
I admit I've never seen any bad comment about her add-ons, so I really don't understand it :-/
Rotherhans wrote: »Quite frankly, she did the right thing.
Obviously she can´t or is simply unwilling to deal with the internet on a more personal level. Which is fine.
So this topic is now done, question is who has the ability to continue?
As one thing is for sure, ZMax surely is far from done messing with the API and they also surely won´t add such a highly needed and useful option to autoloot.
@Sinist nah, I just mostly wanna be able to make stuff for myself without people constantly hounding me for even the smallest change. OH MY GOD YOU CHANGED A TEXTURE, BRB WORLD OVER.
Why can't you just make it do this thing I want?!?!? It's not even that hard, I know because I thought about it


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Former Western SMT MSD407, fleetnumber AD1543 is a Leyland PD3/3 with Alexander L35/32RD new in 1959, seen here at Kirkby Stephen West station on the Settle Carlisle line, during the Kirkby Stephen running day. Some of this batch of PD3/3 chassis had very similar bodies built by Northern Counties.
Photo taken by Eric Bawden, Sunday 5 April 2015, Kirkby Stephen West Railway Station
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Multimedia Projects aims to improve visual Infographic Informations. Create Multimedia Projects with Quality Experts. Inventive Multimedia.
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We are pleased to acknowledge Prof. M. Balakrishnan and Prof. Anshul Kumar for their invaluable guidance during the course of this project work. We extend.
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Project abstracts and downloads for academic mini projects and final year projects A Smart Gateway Design for WSN Health Care System (Electronics Project) Real-Time QoS Routing Protocols in Wireless Multimedia Sensor Networks.
Creating an interdisciplinary team with the right mix of skills is vital to the smooth and successful execution of any project. Team members may be able to cover. While the requirement for Mini Project 3 was to create a simple animation with then added my own code to meet the multimedia requirements of this project. ELEPHANTBOAT RD - P Portable LED Mini Projector Multimedia for . is of sound the sound quality of project is awful. but other things are good.831 :: 832 :: 833 :: 834 :: 835 :: 836 :: 837 :: 838 :: 839 :: 840 :: 841 :: 842 :: 843 :: 844 :: 845 :: 846 :: 847 :: 848 :: 849 :: 850 :: 851 :: 852 :: 853 :: 854 :: 855 :: 856 :: 857 :: 858 :: 859 :: 860 :: 861 :: 862 :: 863 :: 864 :: 865 :: 866 :: 867 :: 868 :: 869 :: 870


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Published on 13 August 2018 - Last update : 10 April 2019
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Four left relatively unscathed following downtown crash where vehicle flipped
CTV News Calgary Published Monday, July 15, 2019 10:30PM MDT Last Updated Monday, July 15, 2019 10:57PM MDT
A CPS member surveys the damage to an overturned SUV following a Monday night crash in the downtown core
Four people suffered minor injuries following a Monday evening collision at the intersection of 9th Street and 5th Avenue Southwest.
According to police, an SUV and a car collided in the intersection and the impact of the crash toppled the SUV.
The three occupants of the Mazda SUV were removed from the wreckage and assessed on scene. The trio were not seriously injured in the crash.
Police officials say the injuries to the driver of the car are considered minor.
The intersection was closed to traffic following the crash. The collision remains under police investigation.


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Although India lost to Australia in the ICC Women's World Cup league match, skipper Mithali Raj achieved a personal milestone. She became the all-time highest run-getter in the history of women's ODIs, at the County Ground, Bristol.
Mithali Raj started off her World Cup campaign in grand style. She created a world record by smashing seven consecutive fifty-plus scores; which makes her the first woman player to achieve the feat. Against Australia, Raj surpassed former England captain Charlotte Edwards's earlier record of 5992 runs when she reached 34* and thereby becoming the highest scorer in ODIs.
Raj reached this landmark in her 183rd ODI, taking 164 innings – 16 less than the former England player, with an average of over 50, with five centuries and 49 half-centuries. The world record run came when she pushed Ellyse Perry's ball towards cover for a single. She achieved the 6000-run milestone in the same match too. She hit a huge six to the leg-break bowler Kristen Beams, after which she received a standing ovation from the Indian bench.
In her 18-year international career, she has gone from strength to strength, smashing one international record after another. The mountain of runs is the testimony to Raj's longevity and unflinching passion towards the game and her team.
"There is an inherent clarity of thought that emanates in her batting. She is technically very correct and is blessed with the gift of timing. She knows when to accelerate and when to defend and is extremely well organized as a person and this personality trait extends to her batting as well. This is precisely what has kept her in good stead all these years," Snehal Pradhan stated about her former captain. "Even in domestic matches, if you don't get her out in the first 20 balls…it's over. She would invariably go on to make a big score," Snehal, who had played against Mithali in numerous domestic games, noted.
As soon as Mithali accomplished the feat, the cricket fraternity came in unison to praise and congratulate her on twitter. Some of them are:
Congratulations, @M_Raj03! Becoming the highest run scorer in Women's ODIs is a huge achievement. Also, superb knock today!
— Sachin Tendulkar (@sachin_rt) July 12, 2017
Wonderful to hear that @M_Raj03 has become the all-time highest run-scorer in women's ODIs. Congratulations!
— Anil Kumble (@anilkumble1074) July 12, 2017
Congratulations Indian run-machine @M_Raj03!
Highest run getter in women's ODIs.
A true champion! #WomensWorldCup2017 #WWC17 pic.twitter.com/9d3K0yuvDI
— Gautam Gambhir (@GautamGambhir) July 12, 2017
Mithali Raj becomes the highest run scorer in Women's ODI Cricket History today. Super achievement ! @BCCI @M_Raj03
— ajinkyarahane88 (@ajinkyarahane88) July 12, 2017
Raj is likely to break another record held by Edwards in the not too distant future. With the 105 games that she has captained, second on the list behind the ex-England skipper's 117. In those 105 games, India has fared better than the fixtures in which she's been back in the ranks. Winning 61 percent as opposed to 53 percent when she has not played as captain.


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Medical students who have moved to the State's North-West were welcomed to the region this week.
The University of Tasmania's Rural Clinical School (RCS) hosted a community event in Burnie on Thursday, 31 January to mark the arrival of the new Year 4 and Year 5 students.
The evening showcased the advantages of living, working and studying on the Coast, and representatives from the community, health sector and government met the students.
Dr Lizzi Shires, Director of the RCS, said she hoped the event would make the students feel welcome and help them settle on the Coast.
"The RCS is excited to be welcoming 54 new and returning medical students to the North-West for the clinical years of their study," Dr Shires said.
"Over the next year, our students will be working in our communities to develop their medical skills and knowledge and work in community-based programs.
"We host a Community Welcome annually, and this year's event was particularly engaging for all involved through an expo-style format.
"Students rotated through different stations, giving them opportunities to meet community groups and find out about the region's culture and activities.
"They also met health professionals, including GPs and many of our alumni who are now furthering their postgraduate training locally as doctors.
"The event is a celebration of all the North-West Coast has to offer, and we hope it shows our fourth and fifth years the many rewarding aspects of where a rural health career can take them."
Fifth-year RCS students who travelled interstate and overseas to undertake summer medical electives presented their experiences at the event.
The recipients of two local scholarships were also announced.
The Simon Monks Memorial Award for Excellence in General Practice supports a high-achieving medical student in their fifth year of study.
The award is presented in memory of local GP Simon Monks and this year has been awarded to Angus Ewing.
The Rotary Club of Wynyard Scholarship in Rural Medicine is awarded to a student with an interest in rural medicine who is commencing the final two years of their study in the North-West.
It was jointly-awarded to Sarah Grace and Sidonie Matthew.


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Copyright © 2020 Two for the Road


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Matcha Cake with Matcha Buttercream
Matcha Cake with Matcha Buttercream
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2018-10-18 test essays you will find explanations regarding the after pages of each and every for the six writeplacer score that is esl accompanied by two test essays which were assigned the indicated score annotations are offered for every essay describing why the essay was handed the rating it received monitoring these sample essays and the accompanying annotations will. 2017-10-23 accuplacer writing prep your score is dependant on your capability expressing, arrange, and help your views and tips the positioning you are taking in the essay subject does not affect your score composing the writeplacer test measures your ability to create effortlessly, that is critical to. Next-generation accuplacer overview all test concerns had been developed after university board's general leading both the classic and new tests make use of 100 point scale nevertheless the range for next papers can be found towards the pupil because they review their specific score report accuplacercollegeboardorg. We very encourage pupils to study/review ahead of evaluating below are some internet sites you will probably find helpful accuplacer introduction for students sample questions accuplacer info accuplacer sample questions accuplacer web-based study app essay / composing instructions accuplacer practice test essay training & resources khan academy(link) purdue owl (website link) guide with test essays (pdf) guide with . 2017-9-20 the next-generation writing placement test is just a broad-spectrum computer-adaptive assessment of test-takers' developed capability to revise and modify a selection of prose texts for effective phrase of tips as well as for conformity to your conventions of standard written english syntax, use, and punctuation next-generation writing|we highly encourage students to study/review prior to testing below are some sites you may find helpful accuplacer introduction for students sample questions accuplacer info accuplacer sample questions accuplacer web-based study app essay / writing instructions accuplacer practice test essay practice & resources khan academy(link) purdue owl (link) guide with sample essays (pdf) guide with . 2017-9-20 the next-generation writing placement test is a broad-spectrum computer-adaptive assessment of test-takers' developed ability to revise and edit a range of prose texts for effective expression of ideas and for conformity to the conventions of standard written english sentence structure, usage, and punctuation next-generation writing as they review their individual score report accuplacercollegeboardorg. Be asked to essay or even a pupils universities to an essay for accuplacer positioning 2007 scored by 50-69 accuplacer essay score 6 best purchase resume cover letter writeplacer test will satisfy this allowed this get your college and practice usually by way of a 120 point scale and accuplacer where accuplacer essay score 6 purchase professional application templates can. 2019-4-16 at first, these accuplacer exam ratings look arbitrary the exam it self is 90 questions, and it is split up between reading, writing, and math questions and an essay at the end the ratings above dictate where a pupil falls regarding the topic proficiency scale centered on. 2019-1-22 accuplacer platform for organizations – the school board login to your platform access is provided to accredited, degree-granting institutions and general public divisions of nationwide, state and system governance that is educational.
Accuplacer test essay test
Accuplacer writeplacer overview and reaction examples test overview the accuplacer writeplacer test will figure out your englishwriting the accuplacer includes the writeplacer exam, that will be the essay that is accuplacer with this part of the test you will be assessed on company, focus, development and help, syntax, and. 2017-4-21 along with other accuplacer test ratings to aid aided by the keeping of students into college-level, developmental or different quantities of esl courses writeplacer esl test-takers are expected to create an essay in reaction to 1 of a few prompts for sale in the machine essays are electronically scored and a score that is holistic 1 to 6, along side. The writing section will undoubtedly be with the reading part and offered a score that is cumulative from 20 to 120 according to where you stand using the test, you might not require any minimum cutoff score alternatively, whatever score you can get will influence which degree of introductory language arts classes you will definitely start at in very first 12 months at university.
2019-4-18 the accuplacer test was created because of the university board that also administers the work test the accuplacer assessments consist of tests from the following subjects: arithmetic, college-level mathematics, primary algebra, reading comprehension, sentence abilities, writeplacer (essay writing) and english as a 2nd language (esl. 2019-4-9 accuplacer tests nevertheless, you could simply just just take so long they express their position scores on as you need to complete each multiple choice test except for the essay, the tests are untimed the position the student takes will not influence their score the essay will be given a holistic score that represents how clearly and effectively.
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Future research plan essay
The outcome of the tests shall help you and nova accuplacer essay score determine the courses you can expect to accuplacer essay rating simply simply take hello, and welcome to the analysis guide area modified saturday positioning evaluation in july and august: campuses pupils with an increase of than 5 years between accuplacer essay score finishing senior school and searching for university, and that do perhaps perhaps not. Test ratings (accuplacer online) will undoubtedly be legitimate for a time period of 24 months through the initial evaluation date pupils whom just take accuplacer at ramapo college and score within re-testing array of the moving score on some of the multiple-choice parts would be permitted just one re-test possibility per test section you are able to re-test one time in the event that you don't place into crwt 102. This essay had been written in reaction to a prompt asking the writer to state his/her viewpoint about community solution hours being needed of all of the pupils for twelfth grade graduation it really is utilized right here just for proofreading training rather than as one example of excellent writing.
Additionally, the author of the straightforward should have adequate points to aid his/her some some ideas which can be contained in the essay a good example for accuplacer essay is beneficial for people pupils who will be finding your way through the test since it enables them to understand the appropriate your essay writers structure of composing an essay therefore the principles on which the essay must certanly be based. Complimentary shipping on begin today you need accuplacer essay score to be able to identify a great thesis when you see one more than 150,000 free practice questions across 200+ subjects including act, sat, gmat, persuasive speech on cell phones cell phone distracts drivers gre, lsat, mcat, common core before we can talk about how to write a great thesis statement, accuplacer essay score. 2014-4-28 interpretation of test ratings for the accuplacer tests™ texas writeplacer essay page 6 the interpretation of test scores is regarded as a few things to consider in putting pupils into regular or courses that are remedial positioning requirements for the institution are unique, it isn't. 2015-9-22 guide to accuplacer placement scores october 2014 comprehension that is reading and answers are supplied just after the essay is submitted a rating of 6 or more is needed for positioning into eng 101 please the reading or english petition permits a pupil to re-test accuplacer for re-evaluation of their reading or.


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Adding or removing a number
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In "Super Circle" you can also add IPKO fixed telephony numbers, as follows: +381387xxxxx


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The nature of our work means we will always make an impact on the local area. However, through our focus on sustainability, we ensure that this is a positive impact, with a careful consideration of six key areas. We engage with the needs of the local community, and our customers who will eventually join this community; we protect the health and safety of our colleagues, as well as their development within the Group; and we seek productive relationships and innovative technologies that ensure our houses and our means for building them last sustainably into the future.
At Inland Homes we are committed to building more than just homes - we strive to create developments that engage and reflect the local community. During the research and planning phase for each development we undertake consultations with the community, discussing the site and its surrounding environment with local groups and town councils. The opinions and requests of both the neighbourhood residents and local government bodies are collected and considered for the brief that the designers and architects base their plans upon. This consultation process means that we can develop the site in a way that best reflects the different needs of the local community.
As well as building a development that will continue to serve the needs of local residents into the future, we are also keen to invest in the community through our engagement with charities, for example sponsoring community sports teams and mini-buses for local schools.
It is of paramount importance to Inland Homes to deliver a high quality service to our customers, from the earliest stages of planning, to after the build is complete.
Our house designs are constantly evolving, based upon feedback from customers on previous projects and our ever growing expertise. We design our developments to meet our customers' needs beyond that of housing, with, for example, outside spaces, children's play areas and gyms. Within the homes, we offer a choice of interior design options to those who purchase our properties in advance of completion.
We manage the handover process, ensuring that the quality of the build meets our stringent standards, and providing introductory home tours for new residents as well as moving in packs with all relevant information. We also co-ordinate aftercare with the contractors, manage feedback and respond to any customer service issues through a centralised system.
It is extremely important to Inland Homes to maintain the highest standard of Health and Safety practices, both with our own employees, and with all contractors involved in our projects. We have rigorous standards which we enforce to ensure safe working practices at all of our sites. Employees have received Health and Safety training, and we require all external contractors who enter our sites to carry a CSCS card at all times to indicate that they have also undergone the necessary training.
We have a strong network of Health and Safety advisers. Additionally, each site is appointed a Construction, Design and Management co-ordinator, who oversees Health and Safety on behalf of Inland Homes. They prepare a comprehensive Health and Safety plan before construction starts, and this is assessed alongside the contractor's Health and Safety and risk plans, before a contract is issued. Once work has commenced on site, we carry out regular Health and Safety audits, as well as assessing monthly reports provided by the contractors.
Inland Homes have relationships with a number of contractors who specialise in different types of build. It is essential to select the correct contractor, both to ensure a high quality result, and to draw on their experience and expertise during the process. There is a dialogue with the contractors throughout the planning and building stages, and we consider suggestions on alternative materials or methods for construction. Using these suggestions has led to saving money and time on several projects, benefiting both us and our contractors, and to discovering new techniques that we may return to in future projects. This co-operation leads to ongoing relationships, which we seek to ensure are mutually beneficial. Our collaborative approach delivers high quality buildings.
As well as developing long term relationships with our contractors, to further our sustainable supply chain we consider the sustainability of materials used on our sites. We clearly specify which materials we permit on our sites, and strive to use sustainable and locally sourced products where possible, as well as experimenting with new sustainable materials.
Planning was gained for 41 units on Vale Road in Bushey, Hertfordshire.
At Inland Homes we constantly seek innovative materials and methods to improve the environmental performance of our properties. We follow each development in legislation and policy that guides sustainability in home building, and ensure that our projects are at the forefront of environmental sustainability.
We include many features in our buildings to minimise their environmental impact, such as rainwater harvesting. We design our buildings to perform throughout the year, with insulation and air-flow optimisation to aid summer cooling as well as retaining warmth in winter. We also seek environmentally friendly materials, both using locally sourced supplies where possible, and experimenting with innovative new technology such as the Durisol block, a building block made of reclaimed softwood, bound with concrete. Comprising of more than 90% recycled material, and generating no waste during manufacture, the block is not only environmentally friendly in production, but also as a feature in homes due to its insulating properties.
Our philosophy of employment is to attract and cultivate raw talent, and encourage people to reach their full potential by offering them opportunities to grow within our open and entrepreneurial culture. We support all of our employees in attending conferences and pursuing qualifications that assist their professional development. Our number of employees has doubled in the last two years to 28, with growth in all departments, supporting our move into housebuilding. We plan to continue measured expansion in the future to complement our strategy, particularly in the establishment of a strategic land department.
As we undertake much of our work through outsourcing, we are also reliant on the continued development of skilled workers outside of the Group. In response to the shortage of skilled labourers, we are involved in initiatives to train the next generation of workers. In some contracts we specify that our contractors must employ apprentices from the local areas. We are setting up a mechanism to connect to local colleges, so that the students can gain the necessary practical experience on our sites to kickstart their careers.
Strategic Report is approved by order of the Board
Paul Brett
15 October 2014
A. Inland Homes plc's new Head Office
B. Peter Kimber – Site Service Manager
C. Tom Cuff – Group Legal Director
D. Keren Birdseye – Architectural Assistant
E. Rob Williams – Assistant Accountant
F. Kathy Hoare – Sales Co-Ordinator
G. John Embleton & Ricky Hayes – DGV site liaison
H. Nikki McCue – Office Administrator
I. Linda Smith – Assistant Accountant
J. Sue Fenton – Office Manager
K. Gary Magee – Planning Assistant
L. Gordon Pearce – Customer Services Manager
M. James Gallagher – Project Manager
N. Olie Pearcy – Trainee Land Buyer
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By Peter Finney Jr. Clarion Herald State scholarship students in six Catholic schools have fallen below the state's educational proficiency benchmarks, Catholic Schools superintendent
Tag: Catholic schools
On top of the world!
Members of the New Orleans-area Eastbank team celebrate after defeating Curacao 8-0 Aug. 25 for the Little League World Series championship in Williamsport, Pa.
New principals named for Catholic schools in 2019-20
High Schools Dr. Tracy Bonday, J.D., M.S. Ed, who served from 2015-19 as superintendent of Catholic schools in the Diocese of Des Moines, Iowa,
Tax credit program: A boon to schools
By Beth Donze, Clarion Herald James "J.T." Hannan has been named the director of advancement for the Archdiocese of New Orleans' Office of Catholic
Sacred Heart School in Norco set to close in May
By Beth Donze, Clarion Herald Sacred Heart of Jesus Elementary School, which has provided a Catholic education to generations of Norco-area families for six


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Volleyball vs. Central Methodist University
October 7, 2019
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Justin Toumberlin is a senior from Eudora majoring in mass media and minoring in psychology. He decided to pursue his love for media after taking video...


text: Teresa started in the Veterinary field as a receptionist at the age of 18. Since then her love for helping animals has grown and so has her knowledge with over 14 years experience working as a Veterinary Technician. She has worked along side general practice veterinarians as well as some of the top orthopedic surgeons. She loves the fact that through the years she can still learn more from the wonderful veterinarians with so much knowledge to give. Teresa currently has two wonderful dogs named "Sally" and "Diesel" that live in harmony with a ragdoll cat named "Lugnut".