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At the start of the August Bank Holiday Weekend, the Irish Coast Guard has issued a water safety appeal asking people to take precautions to ensure their safety, in, near or on the water, both coastal and inland.
The August Bank Holiday weather begins with a marine small craft warning for the West Coast issued by Met Éireann for winds of force six or higher. People who are planning to participate in coastal and water-based activities are encouraged to check the weather forecast. Plan activities based on the forecasted wind and sea conditions to ensure these activities are safe and enjoyable.
It is important to 'Be Summer Ready' and ensure that boats and other water vessels, including kayaks and canoes, are suitable for the forecasted conditions. Ensure that everything is in good working order and that engines have been serviced, with all equipment, particularly lifejackets / personal floatation devices (PFDs), in good condition.
Derek Flanagan, SAR Systems Development Officer at the Irish Coast Guard, commented: "I would like to remind everyone of the need to check the weather and tides before heading out on the water or visiting the coast - there was an unusually wet and windy start to the August Bank Holiday weekend. Exercise caution and take extra time to plan for water-based activities in the coming days, and don't be tempted to take chances if the weather is not as expected."
"Scuba divers and swimmers should wear brightly coloured swimming caps and use surface marker buoys to improve visibility. Swimmers and divers should be aware of the relatively strong tides over the weekend and plan their activities carefully. Always tell a person ashore of your plans and the time you expect to return, and ensure they know what to do and who to call if you don't return as planned. Have a plan to call for assistance if anything goes wrong, call early, don't delay."
If out on a boat or other water vessel, wear a lifejacket, and carry a reliable means of communication – a VHF radio and ideally a Personal Locator Beacon (PLB) or Emergency Position Indicating Radio Beacon (EPIRB) with mobile phone back up. Ensure that you tell someone where you are going and when you plan to be back.
Please refer to the Code of Practice for the Safe Operation of Recreational Craft, which can be accessed at gov.ie - Safety on the water (www.gov.ie). This site also provides important information about water safety, weather and tides and what to do in an emergency situation.
If you see somebody in trouble on the water or along the coast, or think they are in trouble, dial 112 or use VHF radio CH 16 and ask for the Coast Guard.


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Why I Built a Strategic Change Agency
Change happens, by choice or by chance. Proactive or reactive, small tweaks or profound shifts, every business must adapt to survive and grow, especially right now. As my Seattle-based branding and marketing agency helped all kinds of companies create and communicate change over the past 25 years, my team and I came to recognize a common theme. Whether a client came to us seeking a new brand, a new website or a new messaging strategy, it almost always meant that some internal or external shift had happened—or needed to happen.

subset: commoncrawl


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One of my favorite authors of all times is Ray Bradbury. I particularly love his books that are a series of interconnected short stories. One of my favorite TV shows of all times was The Twilight Zone with Rod Serling.
Fantastic Fables of Foster Flat is both my homage to these two favs of mine as well as a bit of a mash-up of these two approaches to storytelling. And it's now available for a very limited time for just 96 cents. (See details below)
In one mountain town, tall tales are that much taller…
"I've seen a lot in my lifetime. You'd think a small mountain town like Foster Flat would be sleepy and boring. Some better adjectives might be strange, surreal, and magical.
I'm Mimi Rawlins. Born and raised in Foster Flat, I've witnessed more than my share of unbelievable incidents here among the mountains. You may not believe some of these stories, but I'm a reporter. I'll tell it to you straight. And I'll promise you one thing: after listening to these stories, your life will never be the same."
Fantastic Fables of Foster Flat is a collection of suspenseful fantasy tales written in the spirit of Ray Bradbury and The Twilight Zone. Inspired by the Great Smoky Mountains themselves, if you like character-driven stories, southern charm, and twists you won't see coming, you'll love Orrin Jason Bradford's assortment of twisty tales.
Stories include:
- Ellenore Finds Her Muse
- 7th Avenue Buddha
- Elliot Savant
- The Diagnostician
- Charm House
- The Gazebo
- Babble
- Birthing Grapefruit
You can order this book from Amazon, B&N, or iTunes for $3.99 and start this exciting adventure in reading, but by buying today direct from the author it can be yours for just 96 cents. Just enter this coupon code during checkout:
(Be sure to select the format of your choice – .mobi(Kindle), epub(Nook,Kobo, etc.) or PDF.)
[wp_cart_button name="Fantastic Fables of Foster Flat" price="3.99″ file_url="https://wbradfordswift.com/oldsite/fffablesdownload" var1="Format|mobi|epub|PDF"]


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This is a Sour - Fruited (View Untappd)
Vault City Brewing says:
'Sometimes ingredients are so unusual and delicious we have to let them stand alone in our Modern Sour Beer. Honeyberries are hardy hybrid fruits which thrive in Scotland, a crazy cross of blueberries and blackberries with a notably 'wild' edge.'
Brewed in Portobello, City of Edinburgh
44cl Cans
Standard shipping is £6Free on orders over £50 or stash until you qualify.
Thanks for Joining!


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"Dark Wave," hosted by Slicing Up Eyeballs' Matt Sebastian, airs 10 p.m. to 1 a.m. Eastern every Sunday on Sirius XM satellite radio's 1st Wave (Channel 33). The most recent two episodes also are available via Sirius XM's On Demand service for online subscribers.
Revolting Cocks, "Attack Ships on Fire" (Big Sexy Land)
The Cure, "A Short Term Effect" (Pornography)
Sex Beat, "Sex Beat" (Sex Beat)
And Also the Trees, "Midnight Garden" (And Also the Trees)
Joy Division, "She's Lost Control" (Unknown Pleasures)
Love and Rockets, "No New Tale to Tell" (Live) (Assorted!)
Siouxsie and the Banshees, "The Passenger" (Loco-Motion Mix) (Through the Looking Glass)
Iggy Pop, "Sister Midnight" (The Idiot)
The Human League, "The Touchables" (Travelogue)
Echo & The Bunnymen, "Pictures on My Wall" (Crocodiles)
Morrissey, "Michael's Bones" (My Early Burglary Years)
Bauhaus, "Dancing" (Mask)
Dead Can Dance, "The Ubiquitous Mr. Lovegrove" (Wake)
New Order, "This Time of Night" (Low-Life)
A Split-Second, "Crash Course in Seduction" (Industrial Mix) (Kiss of Fury)
Johnson Engineering Co., "Floorslammer" (Unleash)
Nine Inch Nails, "Physical (You're So)" (Broken EP)
Ministry, "Filth Pig" (Filth Pig)
Skeletal Family, "Cry Baby" (Cry Baby)
Southern Death Cult, "Fatman" (The Southern Death Cult)
The Comsat Angels, "Missing in Action" (Waiting For a Miracle)
Gene Loves Jezebel, "Cow" (Immigrant)
Slowdive, "Alison" (Souvlaki)
Loop, "Arc-Lite (Sonar)" (A Gilded Eternity)
My Bloody Valentine, "Who Sees You" (m b v)
Ride, "Leave Them All Behind" (Going Blank Again)
The Chameleons, "Soul in Isolation" (Strange Times)
The Smiths, "Suffer Little Children" (The Smiths)
Galaxie 500, "Strange" (On Fire)
Abecedarians, "Misery of Cities" (Eureka)
The Sisters of Mercy, "More" (Vision Thing)
Depeche Mode, "More Than a Party" (Construction Time Again)
The Frozen Autumn, "Winter" (Pale Awakening)
Camouflage, "The Great Commandment" (The Great Commandment)
Nitzer Ebb, "I Give To You" (Ebbhead)
Gravity Kills, "Guilty" (Gravity Kills)
Machines of Loving Grace, "Burn Like Brilliant Trash (At Jackie's Funeral)" (Machines of Loving Grace)
Peter Murphy, "Strange Kind of Love" (Deep)
Got a request? To be considered for next week's show, drop it in the comments below by noon Tuesday. Requests will be considered as long as they fit the show's format and generally within the 1stWave era — and provided the host doesn't harbor an intense dislike of the song.
- Playlist: Sirius XM's 'Dark Wave' — hosted by Slicing Up Eyeballs (1/22/23)
- Playlist: Sirius XM's 'Dark Wave' — hosted by Slicing Up Eyeballs (1/15/23)
- Playlist: Sirius XM's 'Dark Wave' — hosted by Slicing Up Eyeballs (1/8/23)
- Playlist: Sirius XM's 'Dark Wave' — Slicing Up Eyeballs' New Year's Mix (1/1/23)


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You won't miss the alcohol in this refreshing Orange Mocktail Mojito. Made with fresh orange juice and mint, this is sure to please!
This Orange Mocktail Mojito is sweet and minty. Made with freshly squeezed orange juice, lime juice, club soda or clementine soda, fresh mint, and agave syrup, it tastes like an orange mojito without the white rum.
For people who don't want to imbibe, a mocktail mojito is a delicious alternative. This could also be made with rum of course.
Some mojito recipes I've made with rum are a Grapefruit Basil Mojito and a Lemon Mojito Recipe with Ginger.
I've even made mojito-flavored kombucha recipes like Strawberry Mojito Kombucha, Chocolate Mojito Kombucha, and Mojito Kombucha.
Another mocktail recipe to try is my Ginger Pineapple Refresher.
Jump to:
What Is A Mocktail?
A mocktail is a drink that does not contain alcohol. It contains the herbs, sweetener, etc. that make up the cocktail's namesake but is alcohol-free.
🧾 Ingredients
- Orange juice: I used freshly squeezed orange juice. You could also use store-bought. My Orange Creamsicle Kombucha recipe is another recipe using orange juice.
- Lime juice: I used freshly squeezed lime juice. I do not recommend store-bought lime juice.
- Club soda or clementine soda: You could use any sparkling water.
- Mint leaves: I used fresh mint leaves. It's one of the classic mojito ingredients.
- Agave syrup: You could also use simple syrup. You could use maple syrup also, but keep in mind it will add a maple flavor.
To see the full list of ingredients with measurements and instructions, see the recipe card at the bottom of this post.
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🔪 Instructions
- Juice oranges and limes.
- Pour orange juice, lime juice, mint leaves, and agave syrup into a cocktail shaker.
- Muddle the ingredients with a muddler, the flat end of a wooden spoon, or a pestle to break up the mint leaves and combine flavors.
- Pour into a glass over ice and top with club soda or orange soda.
- Garnish with an orange wedge or orange slice.
📖 Variations / Additions
- Maple syrup or simple syrup
- Ginger brew
- Grapefruit, tangerine or other citrus fruit
👩🏻🍳 FAQs
A mojito is a cocktail made with white rum, simple syrup, mint, lime juice, and club soda. A mocktail is a non-alcoholic version of a cocktail.
Since mocktails do not contain alcohol, they are safe for kids to drink.
🥃 Other Drinks
If you're looking for a sweet bubbly non-alcoholic drink, this mocktail mojito is for you.
I originally posted this recipe on June 6, 2015. I added new photos and text when I republished it.
*Don't forget to come back and leave your feedback and star rating.
📋 Recipe
Orange Mocktail Mojito
- Cocktail shaker
- ½ cup orange juice (about 1 orange)
- 2 tablespoons key lime juice
- 6 mint leaves
- 1 tablespoon agave syrup
- ½ cup club soda or sparkling clementine juice
- Add orange juice, key lime juice, agave syrup and mint leaves into a cocktail shaker. Muddle until mint leaves are broken up.
- Pour into a glass with ice.
- Top with club soda or sparkling clementine juice.
*Note: Nutrition information should be considered an estimate only. Different nutrition calculators give you different results.


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Steam game art cannot include extraneous text such as reviews or awards from September 1, in accordance with new guidance from Valve. The Steam developer is aiming to tidy up its storefront with new rules that ban the use of text outside of the game's subtitle and title on its primary art asset.
The new ruleset is aimed at what Steamworks calls "capsules" – the primary banner images that are used to represent games throughout the Steam store. In a post on the Steam community site, Valve lays out their new guidelines for the graphical assets, saying that, "It's our goal to make it as clear and straightforward as possible for customers to find games to buy and play on Steam."
Valve says that an increase in text, award logos, promotional deals, and review scores being applied to these capsules has led to a lack of clarity on the store. The post continues, "Some game logos themselves have become so small that it's hard for players to tell what the name of the game is. In other cases, graphical asset images are so cluttered that it is distracting and hard to read."
The Steam developer also expresses concern that some review scores presented on an image may become outdated in cases where outlets update their reviews over time, which is increasingly common practice in a world of live-service games and downloadable content. As such, the new rules aim to clear up the images presented on Steam's main store pages.
The new Steam "store graphical asset rules" read as follows: "Content on base graphical asset capsules on Steam is limited to game artwork, the game name, and any official subtitle." Valve clarifies further with five additional rules:
- No review scores of any kind, including Steam reviews or external news sources.
- No award names, symbols, or logos.
- No discount marketing copy (eg. no "On Sale Now" or "Up to 90% off" text).
- No text or imagery promoting a different product. This includes no marketing of sequels or other titles in the same franchise.
- No other miscellaneous text.
Valve acknowledges a desire for developers to advertise major updates and seasonal events, and that reflecting these in the capsule art can benefit users. Developers are therefore permitted to use text describing new content such as DLC, battle passes, major updates, and seasonal events as a "capsule artwork override," which can be set to replace the game's usual capsule art for up to one month.
The new rules go into effect from September 1, and Valve has requested that all game developers make any changes needed to bring their art in line with the new guidelines before this date. It adds that "any game not adhering to these rules may have limits to visibility within the Steam store and will be ineligible for featuring in official Steam sales and events," so there's plenty of incentive for developers to ensure that they adapt their art quickly.
Elsewhere in Steam news, a recent PSA highlighted the service's rather nifty controller lighting integration for players who might have missed it.


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Federal Bank
- Branch Thodupuzha
- Address Pulimoottil Roger Roy Towers, Ambalam Bypass Road
Thodupuzha - 685584 - IFSC code FDRL0001121
- Phone No. +914862222232
- Hours
- Closed for the day
- Mon 10:00 AM - 05:00 PM
- Tue 10:00 AM - 05:00 PM
- Wed 10:00 AM - 05:00 PM
- Thu 10:00 AM - 05:00 PM
- Fri 10:00 AM - 05:00 PM
- Sat 10:00 AM - 05:00 PM
- Call Directions
- Branch
- Order Now
About Federal Bank
Federal Bank is a leading Indian private sector bank with a network of 1270+ branches and 1950+ ATMs/Recyclers spread across the country. The Bank offers a wide range of products and services to retail, small and medium enterprises (SMEs), agricultural and corporate customers through a variety of delivery channels. Federal Bank has Representative Offices at Dubai and Abu Dhabi which serve as convenient touch points for its Non-Resident Indian customers in the UAE. The Bank also has an IFSC Banking Unit (IBU) in Gujarat International Finance Tec-City (GIFT City). Federal Bank is transforming itself, keeping its principles intact, into an organization offering services of the highest quality. It has a well-defined vision for the future as a guidepost to its progress.
The Bank offers products and services such as Credit cards, Debit cards, Prepaid cards, Mobile Banking, Internet Banking, Online bill payment, Fee collection, Depository services, Transaction Banking Services, Merchant banking services, Insurance, Mutual fund products and many more as part of its strategy to position itself as a financial super market and to enhance customer convenience.
The address of this branches/ATMs is Pulimoottil Roger Roy Towers, Ambalam Bypass Road, Thodupuzha, Thodupuzha, Kerala - 685584.
Discover More With Us
Scan this QR code to discover more with us.
Other Branches/ATMs of Federal Bank
- Federal Bank branches/ATMs in Kerala
- Federal Bank branches/ATMs in Thodupuzha
Business Hours
- Mon 10:00 AM - 05:00 PM
- Tue 10:00 AM - 05:00 PM
- Wed 10:00 AM - 05:00 PM
- Thu 10:00 AM - 05:00 PM
- Fri 10:00 AM - 05:00 PM
- Sat 10:00 AM - 05:00 PM
- Sun Closed
Parking Options
- Free parking on site
Payment Methods
- Cash
- Cheque
- Credit Card
- Debit Card
- Master Card
- Visa
Nearby Locality
Nearby Federal Bank Branches/ATMs
Federal Bank
No 80F, Animoottil Building
Idukki, Kerala - 685608
Federal Bank
Thodupuzha West
Kalarickal Bazar
Pala Road
Thodupuzha West
Thodupuzha, Kerala - 685584
Near Pvt Bus Stand
Federal Bank
Kunnappillil Building
Thodupuzha East
Idukki, Kerala - 685585
Federal Bank
Chavarattu Building
Thodupuzha Pala Road
Idukki, Kerala - 685586
Federal Bank
Maria Jyothi Square
Idukki, Kerala - 685581
Federal Bank
Ground Floor, Chenthadiyil Building
Idukki, Kerala - 685583
Federal Bank
Thodupuzha Road
Manjalloor, Kerala - 686670
Near Gramin Bank


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Every year as the economy and the sector characteristics change, so should the aim and whole game plan of each student graduating in that particular year change accordingly. If you want to be successful, the first and foremost rule is to be aware of the surroundings and what is happening.
As for the students graduating in the year 2017, the times have become tougher as more and more businesses are going towards digital means and hence you require more skills today for the same job. Because of the digitization of so many services, the lower and middle-level jobs are at the most risk. One at this point not only has to acquire knowledge and skills about their position but also be well versed with the new things in business so as to quickly grab the window of opportunity to climb up the ladder.
One of the most common yet most difficult questions that come in front of students who are just about to graduate is should they study more or take up a job. Now this decision has to be taken based upon many factors. First of all, you need to analyze that what learning opportunities your prospective job provides. Also over here, you may want to inquire with your prospective employer that if the company has any in-house teaching mechanism. If all this is present, a better idea is to take up the job and learn while you are in it.
However, some jobs like say that are related to studies itself or teaching for the matter require you to study in universities later on as well. So in these, it is often suggested that you get done with the studies once and for all.
An apprenticeship is also a good option you have if you want to learn on the job but want some financial security as well. If you land up in a good company, the work may be more but at the same time, the salaries are quite lucrative. Further to this, when choosing a company for your job or apprenticeship you must be very careful. A good plan here would be to get in touch with people who had worked earlier with them and get to know the work culture, their learning experience and similar things. Also, do good research about the company's reputation and what value will an experience over there will fetch you.
NepaliPage,connects Australian Nepalese Community & Australian Nepalese Business Globally
Disclaimer: NepaliPage.com (नेपालीपेज डटकम) is a Nepalese Community website aggregating Nepalese Australian affairs including Australian Nepali Community News, Migration opportunities in Australia, Nepali International Students, Nepali home and garden, Nepali mortgage and real estate tips, tricks, and services, Travel, Entertainment, Nepalese Events, blogs, interview and many more. None of our stories is tailored expert advice for your circumstance, and cannot be taken as legal, migration, or any other expert advice. By nature, all of our contents provide general information on related topics from the various verified sources. We do not offer direct employment opportunities, Australian VISA help, and Migration assistance.


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CCTV Richmond
Video surveillance has evolved into an essential technology for electronic security. Cameras are useful in all aspects of security, life safety and access control. Cameras installed on-site perform two vital functions: deterring would-be miscreants and re-assuring occupants that someone is keeping track of the situation.
CCTV Locksmith provides alarms and CCTV solutions for domestic and commercial clients .
All of our security systems are built with high quality insurance graded components and are expertly installed by us to meet your requirements. With over 10 years experience in the design, installation and servicing of security systems you will be sure we can offer a high level of workmanship and customer care.
We offer all types of CCTV cameras including external, internal, dome, bullet, vandal resistant and covert. Night vision versions of these are also available and they can all be linked to digital recording equipment to capture video data.
As well as our CCTV systems we offer a range of intruder alarm products tailored to your specific requirements. Our systems include wired, wire-free or a combination of both. Pet tolerant systems are a speciality with remote notification options available for all systems. So if you're looking for alarms and CCTV solutions, contact our friendly team now to see how they can help.
We are fully licensed, insured, bonded and ready to assist you as your professional commercial locksmith.
Customer service:
Whether your richmond business is a small office, or a complex with a large amount of sq. ft., we will be able to assist by installing locks to all the doors, be it simple front doors, side doors, back doors and garage doors for your warehouse or unit complex. 24 Richmond Commercial Locksmith Services 804-510-0672 is the first choice in lock and key service provider for you.
Our professional Richmond locksmith services include such things as high security locks, cutting key services, auto repair along with emergency roadside assistance.
We also offer pick a lock services, master key services, CCTV and alarm system installation, door lock services and much more, contact us to learn more about how we can help you.
We are fully licensed, insured, bonded and ready to assist you as your professional commercial locksmith.
Re-key all locks in your business
Provide lock repair/replacement/installation.
Provide key extraction, duplication and master keys.
Install stand alone access control Proximal card readers (Alarm Lock).
Deploy and maintain high security proprietary key control systems for mechanical locks.
Deploy and maintain master key systems.
General maintenance and replacement of "push-pull paddles."
Service and maintenance of crash bars/exit devices.
Replacement and control of keys/locks for desks and cabinets.
Deploy magnetic locking systems for "buzzer entry" of doors (where allowable by law).
Our commercial locksmith services include:
Access control systems
Security camera systems (CCTV)
Alarm systems
Exit devices
Gun and fire safes
Electronic keypads and keyless entries
Panic bar installation
Master systems and high-security locks operations
Changes on safe combination's or new safe installations
File cabinet locks maintenance, repair and/or upgrades
Master key services
Door closers
File cabinet, vault and safe locks
Gates and iron works
Intercom systems
Deadbolt and high security door locks
Plus many more high security locks and key services
If you are looking for a complete installation of locks for all your doors and you also require a security system fully installed for your , office or warehouse, we will be able to assist you. Keep yourself and your property protected from threats and burglars and staff, with a great installation of locks for your doors. We will be able to fully analyze your business and provide you with a great solution to make sure that you do not suffer from any setbacks.
Quality Commercial Locksmith Contractor:
Here at Richmond Commercial Locksmith Company, quality is of utmost importance. We are Commercial Locksmith contractors who not only do new installation of Commercial Locksmith but also repairs on it. And if some Commercial Locksmith needs replacement we can also perform that. Doing the work with dedication is in own top priority charts.
Our personnel are experienced and this experience when brought on the actual work brings fruit, and this fruit is in the form of Service Awards who are the proofs of our utmost dedication.
We always use quality materials in our work and through proper installation and ventilation the overall utility cost is reduced.
Our top quality materials including algae resistant and waterpCommercial Locksmith products keep your roofs healthy for many years. Believe me; work done by us and you will have a life of ease for many a time to come. We provide our facilities in Richmondand further contact info can be found on the website.
No Obligation
Why wait? If you have been thinking about a cctv installation, or repair, give us a call! We know that you will enjoy meeting with our superior craftsman. We are offering you a straightforward, no gimmicks, no-obligation, free inspection and estimate. You won't have to deal with any high-pressure salespeople, and you'll know that you're protected by our superb reputation and 100% guarantee on all our work. Give us a call today at
Products and services
Mark and his crew are the best! Professional, prompt, upfront, communicative, quality work, reasonably priced -- what more can you ask for. They replaced a leaky skylight, repaired parts of my roof, and cleaned out my gutters. I highly recommend Richmond Commercial Locksmith for any roof work you need done!
- martin Thomson, RichmondRichmond Commercial Locksmith is a very professional company. They are on time, do not recommend replacements when repairs are needed, or will do, and they haul away garbage. They discuss options with you, make a recommendation and then execute your preference. They are prepared and efficient at the job site. I've hired them several times and would recommend them to anyone needing roof or related work done, such as replacing clay tile, fixing a problem with a flat composition roof, or re-flashing, or working around the chimney, and gutters. They clean up after themselves so that you'd never know they were on site. They return calls promptly, like same day, usually, no matter if a call placed morning or afternoon. So refreshing dealing with real pros like Richmond
- Mr & Mrs Attric, Richmond


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Type of the house
4 castle
disadvantage1 it costs high if it maintains many rooms well.
disadvantage2 It takes lots of time to build it.
Roma can't be built in a day.
5 container house
It's difficult to obtain a building permit.
In 2 or 3 years, it becomes rust. Rust meams
reddish substans that forms on metal when it comes in contact with moisture, air or water.


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Biomed News
on Discovery and characterization of microproteins
Issue of 2021‒11‒14
four papers selected by
Thomas Farid Martínez
University of California, Irvine
Identification of Non-Canonical Translation Products in C. elegans Using Tandem Mass Spectrometry.
Non-coding RNA-Encoded Peptides/Proteins in Human Cancer: The Future for Cancer Therapy.
A Practical Guide to Small Protein Discovery and Characterization using Mass Spectrometry.
Mosaic translation hypothesis: chimeric polypeptides produced via multiple ribosomal frameshifting as a basis for adaptability.
Front Genet. 2021 ;12 728900
Identification of Non-Canonical Translation Products in C. elegans Using Tandem Mass Spectrometry.
Bhavesh S Parmar
Marlies K R Peeters
Kurt Boonen
Ellie C Clark
Geert Baggerman
Gerben Menschaert
Liesbet Temmerman
Transcriptome and ribosome sequencing have revealed the existence of many non-canonical transcripts, mainly containing splice variants, ncRNA, sORFs and altORFs. However, identification and characterization of products that may be translated out of these remains a challenge. Addressing this, we here report on 552 non-canonical proteins and splice variants in the model organism C. elegans using tandem mass spectrometry. Aided by sequencing-based prediction, we generated a custom proteome database tailored to search for non-canonical translation products of C. elegans. Using this database, we mined available mass spectrometric resources of C. elegans, from which 51 novel, non-canonical proteins could be identified. Furthermore, we utilized diverse proteomic and peptidomic strategies to detect 40 novel non-canonical proteins in C. elegans by LC-TIMS-MS/MS, of which 6 were common with our meta-analysis of existing resources. Together, this permits us to provide a resource with detailed annotation of 467 splice variants and 85 novel proteins mapped onto UTRs, non-coding regions and alternative open reading frames of the C. elegans genome.
C. elegans; LC-MS/MS; MSFragger; PEAKS; altORFs; mass spectrometry; sORFs; timsTOF
Curr Med Chem. 2021 Nov 11.
Non-coding RNA-Encoded Peptides/Proteins in Human Cancer: The Future for Cancer Therapy.
Seyedeh Zahra Bakhti
Saeid Latifi-Navid
Although non-coding RNAs (ncRNAs) were initially thought to be a class of RNA transcripts with no encoding capability, it has been established that some ncRNAs actually contain open reading frames (ORFs), which can be translated into micropeptides or microproteins. Recent studies have reported that ncRNAs-derived micropeptides/microproteins have regulatory functions on various biological and oncological processes. Some of these micropeptides/microproteins act as tumor inhibitors and some as tumor inducers. Understanding the carcinogenic role of ncRNAs-encoded micropeptides/microproteins seems to pose potential challenges to cancer research and offer promising practical perspectives on cancer treatment. In this review, we summarized the present information on the association of ncRNAs-derived micropeptides/microproteins with different types of human cancers. We also mentioned their carcinogenic mechanisms in cancer metabolism, signaling pathways, cell proliferation, angiogenesis, metastasis, and so on. Finally, we discussed the potential clinical value of these micropeptides/microproteins and their potential use in the diagnosis and treatment of cancer. This information may help discover, optimize, and develop new tools based on biological micropeptides/microproteins for the early diagnosis and development of anticancer drugs.
Cancer; CircRNAs; LncRNAs; MiRNAs; Micropeptides; Microproteins; NcRNAs
J Bacteriol. 2021 Nov 08. JB0035321
A Practical Guide to Small Protein Discovery and Characterization using Mass Spectrometry.
Christian H Ahrens
Joseph T Wade
Matthew M Champion
Julian D Langer
Small proteins of up to ∼50 amino acids are an abundant class of biomolecules across all domains of life. Yet, due to the challenges inherent in their size, they are often missed in genome annotations, and are difficult to identify and characterize using standard experimental approaches. Consequently, we still know few small proteins even in well-studied prokaryotic model organisms. Mass spectrometry (MS) has great potential for the discovery, validation, and functional characterization of small proteins. However, standard MS approaches are poorly suited to the identification of both known and novel small proteins due to limitations at each step of a typical proteomics workflow, i.e., sample preparation, protease digestion, liquid chromatography, MS data acquisition, and data analysis. Here, we outline the major MS-based workflows and bioinformatic pipelines used for small protein discovery and validation. Special emphasis is placed on highlighting the adjustments required to improve detection and data quality for small proteins. We discuss both the unbiased detection of small proteins and the targeted analysis of small proteins of interest. Finally, we provide guidelines to prioritize novel small proteins, and an outlook on methods with particular potential to further improve comprehensive discovery and characterization of small proteins. IMPORTANCE Small proteins of up to ∼50 amino acids play important physiological roles across all domains of life. Mass spectrometry is an ideal approach to detect and characterize small proteins, but many aspects of standard mass spectrometry workflows are biased against small proteins due to their size. Here, we highlight applications of mass spectrometry to study small proteins, emphasizing modifications to standard workflows to optimize the detection of small proteins.
FEBS J. 2021 Nov 07.
Mosaic translation hypothesis: chimeric polypeptides produced via multiple ribosomal frameshifting as a basis for adaptability.
Umut Çakır
Noujoud Gabed
Marie Brunet
Xavier Roucou
Igor Kryvoruchko
How many different proteins can be produced from a single spliced transcript? Genome annotation projects overlook the coding potential of reading frames other than that of the reference open reading frames (refORFs). Recently, alternative open reading frames (altORFs) and their translational products, alternative proteins (altProts), have been shown to carry out important functions in various organisms. AltORFs overlapping refORFs or other altORFs in a different reading frame may be involved in one fundamental mechanism so far overlooked. A few years ago, it was proposed that altORFs may act as building blocks for chimeric (mosaic) polypeptides, which are produced via multiple ribosomal frameshifting events from a single mature transcript. We adopt terminology from that earlier discussion and call this mechanism mosaic translation. This way of extracting and combining genetic information may significantly increase proteome diversity. Thus, we hypothesize that this mechanism may have contributed to the flexibility and adaptability of organisms to a variety of environmental conditions. Specialized ribosomes acting as sensors probably played a central role in this process. Importantly, mosaic translation may be the main source of protein diversity in genomes that lack alternative splicing. The idea of mosaic translation is a testable hypothesis, although its direct demonstration is challenging. Should mosaic translation occur, we would currently highly underestimate the complexity of translation mechanisms and thus the proteome.
alternative open reading frame; chimeric protein; mass spectrometry; protein coding potential; ribosomal frameshifting; translation


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The marker shows the location of Queen Elizabeth National Park, at (-0.712, 29.6301)
Zoom in and out with the buttons. Click on the map and drag it around.
Species recorded at Queen Elizabeth National Park:
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What is web hosting provider? A service provider that offers you space to publish your websites is known as a web host. Most of the websites are launched for different purposes ranging from personal identity to corporate identity. Today there are countless personal websites especially for your family and friends. These personal website staff will… Continue reading
Month: October 2022
Get high exposure for your website with link exchanges
There are many methods for a website to gain high exposure; One of them is through link exchanges. You can start by using your main keyword. For example, you'll use "WordPress themes" as your primary keyword, since that's what your website will be about. If you're going to do a search on Google, you can… Continue reading
Turn household trash into cash
Turning household trash into cash is not as difficult as you might think as there are now many ways to do it. While you may look around your house and see nothing but clutter and trash, what you may actually be looking at is cash. Remember, one person's trash is another person's joy and one… Continue reading
How to deal with complaints: Isn't one year enough?
There are things we don't want to happen but have to accept, things we don't want to know but have to learn, and people we can't live without but have to let go. ~Author unknown Grievance is as personal and individual as any other part of our lives. And if you've never experienced it, you… Continue reading
What is the difference between venture capital and working capital?
It's not uncommon for business owners experiencing a cash flow crisis to determine that bringing in a partner or equity investor, such as a venture capitalist or angel investor, will solve all of their problems. Unfortunately, during my 28 years in the alternative trade finance industry, I have seen many businesses fail due to this… Continue reading
Badminton! Fun and worth playing!
One can easily accept that the game of badminton is fun, but question whether it is worth playing! Let me reassure those who are skeptical about the use and value of this amazing healthy game. Every sport has its own positives and negatives. But when it comes to badminton, one finds it difficult to single… Continue reading
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Most companies strongly differentiate between content and marketing. They perceive marketing as the distinct process by which they sell their content. (Note: content in this context may refer to any product and/or service). They believe that giving away content is a money wasting exercise. This thinking is counterintuitive. If your prospect doesn't get a chance… Continue reading
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ED, or erectile dysfunction, affects more than 50% of men over the age of 50, and as men age, that number rises dramatically. The blue pills have become one of the best-selling medications in the world due to this condition. However, all blue pills have unpleasant side effects and some men cannot take them due… Continue reading
First Step to a Successful Startup: 12 Ways to Eliminate High Startup Loans
When it comes to startups, the common problem most entrepreneurs face today is money to start a business. Being an entrepreneur is not easy, you have to take risks and move forward with the psychology of optimism. You need to think of ways in which you can get the most out of the least input.… Continue reading
The challenge of writing an artist statement that is artistic and profound but also makes sense
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It's enough to turn anyone's stomach but a new treatment that transplants faecal matter from one person to another could help to save lives.
The procedure, which is already being used by some doctors, repopulates the patient's gut with the healthy bacteria that can become unbalanced after the use of antibiotics.
Dr Alisdair MacConnachie, from Gartnavel General Hospital in Glasgow, carries out the procedure in the UK for Clostridium difficile infection, a disease that can be caused when healthy bacteria are wiped out from the gut by antibiotics.
The theory is that by adding more bacteria to the bowels, they will compete with the Clostridium difficile bacteria and control the infection.
He says it is a proven treatment but should only be used as a last resort.
"My personal view is that this technique is there for patients who have tried all the traditional treatments," Dr MacConnachie told the BBC.
"If a patient doesn't respond to that and still gets recurrent C. difficile then they're in real trouble and there isn't really any other technique or any other treatment that has the proven efficacy that faecal transplant does."
Dr MacChonnachie has performed the procedure over 20 times and says it was successful in removing the bacteria in all bar one of the cases.
He told the BBC that other doctors may be more hesitant to embrace the treatment because of its repellant nature:
"It sounds disgusting, it is disgusting and I think people are probably worried about approaching patients and discussing it."
For optimum results the donor of the faecal matter would be a relative who lives with the patient; living in the same environment and eating the same food means they are more likely to have similar bowel bacteria.
The donor comes to the hospital on the morning of the operation and produces a sample. About 30g of this is blitzed in a household blender with salt water and poured through a coffee filter to leave a watery liquid.
To transfer the liquid into the patient's bowels, Dr MacConnachie inserts a tube up the nose and down to the stomach and pours the liquid through the tube.
The treatment is growing in popularity in the States where doctors believe it could be used to treat common complaints such as irritable bowel syndrome, diarrhoea and constipation.
However, until the necessary clinical trials take place, it is unlikely the treatment will become widespread in the UK.


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Published: in Videos
How to install Asterisk PBX on FreeBSD
First in a series of videos taking a look at Asterisk. I take a look at how to install the Asterisk open source communications framework on FreeBSD using binary packages. Asterisk is used as the basis for many existing PBX solutions.


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Scientists develop a flexible carbon nanotube battery to go with flexible displays
Researchers from the New Jersey Institute of Technology have developed a battery that can be folded and bent without exploding. This could finally make foldable devices a reality.
Ryan Whitwam
Why curved smartphone screens massively increase image quality, daylight readability
Believe it or not, the curved screen on the Samsung Galaxy Round smartphone isn't just a marketing gimmick: The curvature actually causes a series of optical effects that result in improved contrast, color accuracy, readability, and overall image quality -- especially under ambient light that usually makes smartphone screens almost unreadable, such as daylight or fluorescent office lighting.
Samsung unveils curved Galaxy Round smartphone, innovates nothing
Whereas Apple sticks to a core device design and ideal, Samsung creates as many different device designs as possible, from flip phones named after Irish alcohol, to phablets that are only fractions of an inch smaller than a traditional seven-inch tablet. Now, Samsung has unveiled the first smartphone with a curved screen, the Galaxy Round, confirming that there's no point to a slightly curved smartphone display.
James Plafke


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The faculty members are actively engaged in the following key research areas and publish their papers in well-reputed journals.
- Mechanical Behaviour of Engineering Materials
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- Prof. Dr.-Ing. Furqan Ahmed
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- Dr.-Ing Muhammad Zubair
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(click on the names to see the research profiles)


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Making Use Of the CB Insights platform, the research team picked these 250 winners from a pool of over 12,500 personal business, including applicants and candidates. They were picked based on aspects including R&D activity, proprietary Mosaic scores, market capacity, service relationships, financier profile, news belief analysis, competitive landscape, group strength, and tech novelty. The research study team also examined over 2,000 of Expert Rundowns sent by applicants.
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has now been acknowledged on CB Insights' Fintech 250 two years in a row.
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Equity financing and deals: The Fintech 250 mate has actually raised over $115B in equity funding throughout over 1,100 deals given that the start of 2017 (since 9/20/2022). In 2021 alone, winners raised over $51B across 337 equity offers.
Unicorns: There are 159 business with $1B+ evaluations on the list.
Global Reach: Globalization is an essential theme for this year's Fintech 250. The winners represent 33 various countries around the world – 7 more than in 2015. Simply over half (53%) of the chosen companies are headquartered in the US, which is the least we've seen in the Fintech 250 because 2017. The UK was available in second with 31 winners (12%), followed by India with 14 (6%), Brazil with 9 (4%), and Germany with 7 (3%).
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Union Budgets are always hits and misses for all Finance Ministers. On February 1, 2023, as FM Nirmala Sitharaman presented her 5th and last Budget for PM Modi's 2nd tenure Government, it is important to note that the budget holds extremely high significance in wake of multiple factors and constraints. These includes pre-election Budget, critical in time when there are no signs of Russia-Ukraine war ending, and concerns of growth in wake of economic crisis in the West. Any untoward volatility in crude price or exchange rates holds the potential to hurt the economy. Amid such compulsions, the Government is also sitting on benefits arising from tailwinds like higher than projected tax collections and higher tax buoyancy along with an encouraging economic growth outlook.
Tax buoyancy has helped the Government in augmenting higher than budgeted tax revenues. This was backed by a better than expected economic recovery, backed by strong surge in industrial production and exports. Success of Productivity Linked Incentives (PLI) schemes in reducing imports and making Make In India and atmanirbhar Bharat possible along with promoting exports of products like Mobile Phones that India was importing has increased confidence of the Government in pushing PLI schemes in more sectors.
While lot of dissection of the Budget is done, this article endeavours to evaluate it from the prism of three major forward looking themes and its likely impact on the economy.
Capital formation driven economic growth
In the first Budget after Modi 2.0 Government got reelected in 2019 elections, the outlay for infrastructure stood at Rs. 3.4 lakh crores. Since then the Government has increasingly focused on increasing the budgetary allocation for infrastructure to touch Rs 7.5 lakh crores in Union Budget 2022 and this year it further increased by 33 per cent to touch Rs 10 lakh crores. If we look at the full tenure of this Government, this is nearly a threefold jump in budgetary allocation. This has an impact on the economic growth. Capital formation led growth has been Bharatiya Janata Party (BJP) led–NDA Government's ever since the Vajpayee years. This results in trickledown effect with increased demand in downstream sectors like steel, cement, construction materials, equipments, achinery, transportation industry, and most importantly employment of unskilled and semiskilled labour. Modi Government has continued this path. This in turn has a further trickledown effect on sectors like FMCG, white goods, and automobiles.
Rural growth
Modi Government's multiple initiatives like Agribusiness Incubation Centres and Unity Malls are path breaking and revolutionary ideas. After withdrawal of Farm Bills, this is the first positive step forward in the right direction to help farmers to augment their income. By setting up Agribusiness Incubation Centres that will focus on startups in the agri sector, farmers will be able to find customers in the vicinity to sell their produce at a relatively better price compared to adatiyas. Similarly setting up Unity Malls in every State capital wherein products with GI, products made under 'One District One Product' scheme, and other handicraft products will be a big move to expand the market of these products by setting up distribution network across the country.
This will also help in creating employment opportunities in rural areas, which is currently dependent on agriculture or MGNREGA for employment. Rural India faces challenges of unemployment and overdependence on agriculture to create employment.
Promoting green economy
In 2021, speaking at Glasgow COP-26, PM Modi made an ambitious target of India becoming carbon neutral by 2070. If PM Modi's track record ever since his term as CM of Gujarat has to be examined, it is visible that he had a special attraction towards green energy. During his term as CM of Gujarat, he focused on solar and wind energy. That was the period when solar panels were quite costly, and then too the then CM Modi focused on installing solar panels in many areas, including covering the Narmada Canal with solar panels to generate green energy and saving water by stopping evaporation. It is quite possible that PM Modi's love for environment–friendly energy made him set aggressive and ambitious targets for India.
Modi Government's multiple initiatives like Agribusiness Incubation Centres and Unity Malls are path breaking and revolutionary ideas. After withdrawal of Farm Bills, this is the first positive step forward in the right direction to help farmers to Augment their income
However, looking at recent developments be it promoting electric vehicles to developing railways operating on green hydrogen to many other small initiatives like "battery as a service" proposal in Union Budget 2022, it is evident that Central Government is extremely committed to achieving targets set by PM Modi. The FM has announced an outlay of Rs. 19,700 crores for National Green Hydrogen Mission to facilitate the transition of the economy to low carbon intensity, reduce dependence on fossil fuel imports, and make the country assume technology and market leadership in this sunrise sector.
Winning middle class approval
Middle class was miffed for a long time watching lot of schemes for poor. Being the lost Budget before the election year, there were high expectations from the middle class on getting some sops. FM Nirmala Sitharaman has very smartly offered sops to the middle class by tweaking tax slabs and making income upto Rs. 7 lakhs tax free for middle class. This is done without making the same tax benefits available for people with an income above Rs. 7 lakhs. The FM has also focused on making the new tax regime more attractive for the middle class. Till last year, the new tax regime was not really very attractive for middle class and they continued to opt for the old tax regime. This year, we can expect the scenario to change.
To sum up, the Budget is a forward looking budget that balances electoral populism with economic pragmatism.