text:
I built & sold my crypto startup and now building a scheduling app remotely
Founder at @MeetCalbot & former founder at CryptoDaddy who is working remotely
1. Hey, can you please introduce yourself?
Hello 👋🏻 I'm Taras! I'm a founder and director of a startup called Calbot. We make a bot that schedules meetings for you and also protects your schedule and time to give you deep work. I've been in the UK for 11 years, and in the past 5 was studying Management and Computer Science at the University of Leeds. It was a great mix of subjects!
Whilst at university, I also ran another business called Crypto Daddy which is an online store for crypto-related merch. It was a lot of fun and I really enjoyed the dynamism and agility of the crypto scene, but that business was very different from what I do now. In 2018 I sold Crypto Daddy to focus on my last year of university. This also gave me time to apply for the Tier 1 Exceptional Talent visa which allowed me to stay in the UK after I graduated to start a software company which is something I always wanted to do 😁
2. What motivated you to choose remote working?
I never really liked working in an office. Sitting in front of a computer all day was super boring for me and I wanted a bit more movement (both physically and metaphorically) in my life. I remember reading about Parkinson's Law which says that "work expands so as to fill the time available for its completion" and thinking that my time in the office is exactly that.
I could complete the tasks from management quickly and be done with it, but then they would give me more stuff to do, so why would I? Plus, the job was 9-5 and I couldn't just leave earlier if I was done with everything, so again, why would I do the job quickly?
As a result of that discontent with the "traditional" working environment I knew that I wasn't going to be a good "traditional" worker (nothing wrong with it, it just wasn't for me!). And so, I started looking for ways to work on my terms. Turned out that starting my own business and doing it remotely was the answer!
3. What were your initial months like? Did it live up to your expectations?
I thought that working on my own business and working remotely was going to be amazing, but the first few months were actually a bit tough. I felt very guilty for waking up late and not working at the time "when you are supposed to".
However, slowly, afterwards, I started to get into it and stopped feeling guilty about finishing work early or starting work late. The thing that helped me overcome it was, again, Parkinson's law. I realised that I was "packing" a lot more work done into 1 hour with a 4-5 hour work day than I was in 1 hour in an 8 hour work day in the office.
This gave me more time to do activities outside of typical working hours without feeling guilty about them. For example, I started running between 3-4pm everyday in the park, which was brilliant, because the park is fairly empty at that time because everyone is at work. I can imagine this is also the case for gyms, swimming pools, etc. This change in outlook has completely shifted the way I look at time and helped me get into remote working more.
4. What have been the best, good and worst aspects of remote working for you?
I think the freedom it gives you is the best thing. I can work from anywhere in the world (subject to WiFi availability) and at any time which is awesome. In the last 8 months I visited Vilnius and Trakai in Lithuania, Krakow in Poland, Belfast in Northern Ireland, many different places in Iceland (obligatory northern light photo attached) and am soon going to Southampton.
But it is not all sunshine and rainbows. I think the worst part of remote working is the inability to disconnect. Because of different timezones people might message you early in the morning or late in the evening and you just have to reply because otherwise it will bug you for the rest of the day/ night.
I learnt to just turn notifications off for my emails, Messenger, WhatsApp, Slack, etc, and this helps a lot. Plus, I (obviously) use Calbot for scheduling meetings, so I never have to wake up at awkward hours for a video meeting or call.
The other annoying bit of remote working is visas and laws. I feel immigration laws are very much outdated when it comes to working remotely abroad. Unnecessary laws, outdated tax-policies and visa requirements are ruining what could be a world where people can come into the country for both work and leisure. Otherwise, at the moment, you need to get a tourist visa or a work visa. But then, if you stay in the country for more than 90 days and they suspect you are working there they might want to tax you on your income.
5. What tools do you swear by while working remotely?
Our team uses Slack for communication and I always have it open on my computer. However, their mission of "killing" is far from being achieved. Everyone still uses email and they will no matter what Slack does.
My email client of choice is Spark. It's clean, has delayed send features, reminders, templates and helps me keep my inbox tidy. However, I am still mad at Google for killing Inbox!
Microsoft ToDo is brilliant as well. I tried Notion as well and I didn't get it. Too many features and too bloated. Is it a todo list? Is it a note-taking app? Who knows? MS ToDo is simple and clean. That's all I need.
My browser of choice is Brave. It's based on Chromium and so has all the extensions and plugins, but it's noticeably faster and doesn't spy on you.
Microsoft OneNote is awesome as well. It's simple and clean and has a straightforward system of sorting notes.
And I also use Calbot for scheduling meetings. Unlike Doodle or Calendly it is amazing at scheduling all types of meetings. Instead of asking people to vote on their availability, whilst you pray that everyone votes for the same slot, it filters down on each participants' availability to find a slot that works for everyone. Plus, we are also testing a feature that protects your time and allows you to do deep work instead of being constantly distracted by meetings.
6. What is your golden advice to a new remote worker?
Plan your time very well. I really like planning every minute of my day (which could be too much for people), but it is essential that you know exactly when things need to be done and how long it will take you to complete them. Block off time in your calendar for work and make sure that that time is in one big chunk. Eliminate all distractions and hold yourself accountable for the deadlines in your work.
7. How do you expect remote working to evolve in the future?
Hopefully more people will get into it and it will become more commonplace. Corona has definitely given it a boost as more people have realised that they can actually do the same job, but from home (or from anywhere else).
Maybe, because of so many people working from home, we will also, finally stop being the slaves of Parkinson's law which forces us to spread our work between a typical 9-5 and actually get to the Keynesian 15-hour work week?
I also hope that governments around the world will realise that current taxation, visa, immigration, data protection and work laws are completely incompatible with remote working. I think we are going in the direction where more people, teams and companies are becoming remote. Yet, things like EU's data protection laws are clearly holding us back by enforcing unnecessary and bureaucratic regulations that only make life more difficult for startups, remote workers and remote companies.
8. Where can we follow you on?


text:
"Lets sleep for a while." As soon as he finished speaking, He Xun was so angry that he wanted to bite off his tongue.
What was he saying It didnt seem right to invite her to sleep on the bed with him after what had happened.
He honestly wanted to say that he would take a nap!
He Xuns adrenaline had soared from the stimulation just now.
He truly wanted to turn into a wolf and make the best of the situation.
He…he didnt dare to have such a thought when he looked at that fair little face.
His baby was so pure.
How could he take advantage of her
However, He Xun didnt know that his pure and precious wife, Chen Weier, couldnt wait to push him onto the bed and take off his clothes…
No one knew where the couples misunderstanding of each other had come from…
Of course, even though she was thinking about it, Chen Weier would only dare to seduce her husband and force herself on him.
She might even need to add two bottles of liquor to boost her courage! Her charm really cant eat He Xun.
But now, when Chen Weier heard He Xuns words, she threw her clothes aside and happily pounced on the bed.
He Xun saw something shaking under the translucent shirt.
He wanted to have a nosebleed again.
He Xun felt as if he had personally dug a hole and then jumped into it, even burying it with some soil.
Chen Weier hugged He Xuns arm and rubbed against it.
"Then, lets take an afternoon nap together."
He Xun wanted to say, "Not good." But when the words came out, they became, "Good."
He Xun spurned himself in his heart, but his body obediently lay beside Chen Weier.
Chen Weier wasnt sleepy.
She kept rubbing her head against He Xuns body, and her small hands kept touching here and there.
He Xuns heart was numb.
"Dont rub around anymore!"
"Why" Chen Weier pouted her mouth in dissatisfaction.
She was excited and wanted to move around.
He Xun looked at her and felt that she seemed to be even more excited.
He held his breath and said, "Theres no reason.
Close your eyes and sleep."
"I cant sleep." Chen Weier mumbled.
"You just said that we are taking an afternoon nap together.
You must be tired.
Hurry up and sleep." He Xuns body stiffened.
"But Im not sleepy when I lie down.
Hubby, I cant sleep…" Chen Weier raised her tiny head and acted coquettishly.
Her soft and tender voice made his body go soft.
He Xun felt that he couldnt go on like this.
It was too deadly.
He started to recall the news broadcast in his mind before he calmed down.
Then, he pinched Chen Weiers tiny face.
"Since you cant sleep, then dont sleep.
Lets talk about what happened in the company today."
Chen Weier was speechless.
So, who exactly was He Xun Was he made of steel
"Be good.
Lets take an afternoon nap." Chen Weier was silent for a moment.
"No, youre not sleepy.
So, why did you fight with them" He Xun turned her face back to face him.
Chen Weier knew she couldnt avoid it and immediately looked at him with dissatisfaction.
"Its all your fault.
Look at what kind of people you have in your company.
Theyre spouting nonsense!"
She had to take the moral high ground first.
As expected, He Xuns face revealed a guilty expression.
"Im sorry.
Its my fault.
There wont be any more of this in the future.
Ive already asked Yang Zui to fire them."
"Hmph!" Chen Weier glared at him and then poked his stiff arm.
"And whats with that Luo Xinrui Dont you know that everyone in your company is saying that shes the real Madam He She said she would come to the company several times a month! You guys had a great time chatting and were so gentle to her! Shes the woman whos been in and out of your office the most!"
Chen Weier added oil to the fire, determined to push all the mistakes to He Xun, trying to make him forget her embarrassing incident today.
He Xun felt he had dug a hole for himself again, so why did he mention this matter "Im sorry.
She wont appear again." He Xun had never coaxed anyone before, so he didnt know how to coax Chen Weier.
He was clumsy and didnt know what to do at this moment.
He only knew how to keep apologizing.
"Hmph!" Chen Weier pretended to be angry and said, "They stood in front of me, ridiculed me, and flattered her.
They said that she was your wife.
She was the person in your heart.
They even said that you pampered her a lot! You cant wait to see her every day." Chen Weier felt she had added too much and didnt know how to end it.
"I didnt." He Xun felt wronged.
"She only comes to report her work every month.
I only give her 20 minutes.
Once the time is up, I let her go.
Ive never talked to her about anything else during work hours! Ill disclose your identity today, okay"
Thank you for reading on myboxnovel.com

subset: commoncrawl


text:
Image: Samantha Robinson Handmade Porcelain
The Gallery Gift Shop, located on the ground floor of Wollongong Art Gallery, offers a range of book titles, and art journals, as well as unique gift ideas. The shop also has a range of postcards and greeting cards to suit most occasions.
Our stock changes frequently in line with our exhibition program, and many items are only available for a short time. With a variety of products that are perfect for gifts, or just to treat yourself, it's worth dropping by regularly to see what is new.
Image: Aboriginal dot painting reusable masks with 3 layers designed by Lorraine Brown and Narelle Thomas
Image: Keep your drinks cool with these stubby holders
Image: An assortment of Coomaditchie cards and magnets from $5.
By becoming a Wollongong Art Gallery Friend you will receive a 10% discount on all shop purchases.
Educations Kits are available from the Gift Shop priced from $5. Kits include:
Credit card facilities are available for MasterCard and Visa.


text:
Do you realy get a sense that your particular matrimony was losing apart? Will it feel the fresh jobs you're making discover your matchmaking straight back focused try futile? Imagine youve attempted what you?
There are lots of conditions your location better off not seeking to save their relationship . These include next several huge warning flags:
- Your spouse is actually in person or emotionally abusive for your requirements and/or youngsters.
- Your wife lies, hacks, otherwise means shady choices.
Thereupon out of the way, lets view some traditional facts you to definitely occur in marriages which might be towards decline and lots of an effective way to fix them.
Your own brief issues apparently usually intensify on grand arguments
You are both from the point where it looks like every conversation results in a fight. You are worn out, seeking keeps a municipal, respectful conversation.
Whats taking place here is there exists various strong-seated resentment and you will unexpressed fury . After you a couple of perform take part (even when this isn't on the an interest that's always unpleasant), something quickly become heated.
It provides to help you hide the fresh "real" anger that isn't are conveyed. The constant attacking deflects in the genuine affairs below that you could work with the resolving yet , never ever totally overcame.
You need to be capable freely and you can pleasantly share brand new rage you have already been harboring, and your spouse must be able to hear this in place of flying from the deal with. (An equivalent for you, too.)
With the aid of a therapist, you can learn how to approach these delicate products from inside the a good method in which actions your for the solution rather than to your all of the-out disagreement.
After you remember your ex partner, this isn't with a sense of like or happiness
Whenever a married relationship was losing apart, it is hard to think about your partner into the enjoying terminology. When you replay a discussion with these people, you're more likely to end up being anger, maybe not love.
You would imagine exactly what it was need to get off him, simply how much best off youd become. You have got a difficult time discovering a fantastic, enjoying believe into your. The times away from giddy daydreaming concerning your mate is actually gone.
Book a consultation which have a marriage therapist and then have prepared to perform some essential performs, the original becoming to choose if your affairs are reconcilable.
You really have zero need to try to make your companion happier
In which when you spent an hour or so considering what clothes so you're able to don which have him, now you spend the nights and you can weekends from inside the sweatpants as well as your old college hoodie?
Do you really no further perform some absolutely nothing niceties one demonstrated just how far your adored him, such as for instance providing your a walk in the morning or planning his favourite sub getting his food?
The deficiency of becoming reasonable to your companion are an indication that you will be annoyed with your and you may cannot need certainly to delight your. You're holding right back while the he or she is unpleasant or unsatisfactory your.
Instead of cover up behind the newest monitor of simply overlooking your partner, you need to have the dialogue supposed on which is really significantly less than this decisions?
Again, on relationships advisors workplace, you'll have a guided dialogue throughout the the reason why you no further feel like carrying out things nice getting him .
"Why must I bump myself out by getting ready an effective eating for us as he never ever also states many thanks," is a good first rung on the ladder. (It may prompt him to keep in mind you to definitely expressing appreciation close by and your services is an essential part http://datingranking.net/it/incontri-bisessuali a good matrimony.)
You feel zero union
Have you ever for every set-up separate hobbies, groups of family members, affairs that you manage outside the home one to try not to cover the other?
And you will worse, do you really never ever go back together to express what you're undertaking you should definitely along with her? Do him/her genuinely believe that simply being in an identical place with you but on the computers otherwise mobile phone function you're hanging out together, whereas you long for the days whenever youd speak with her for each and every nights?
Telecommunications is necessary here. "I believe like we arent hooking up in every meaningful means" is an excellent terms to open it talk. (Once again, most readily useful done in this new safe space off a wedding counselors place of work.)
If the spouse thinks things are fine and won't wanted to change things to become along with you way more, it would be time to let this matrimony go.
It might feel like as the drift has actually happened, their impractical to come back to as the enjoying spouse. Although not, on the proper amount from effort and time, things are bound to return to normal, and save your incredible marriage.


text:
Some days, absolutely nothing makes a hit like a curry. And also, in Dublin, you're spoiled for selection. Sure, you'll locate bog-standard takeaways spread all over the city. Yet, Ireland's funding also boasts quirky, genuine hole-in-the-walls serving bona fide Indian meals and also super-swish areas where you can take pleasure in a roof alcoholic drink together with your dhal.
9. Vermilion Indian Cuisine
Positioned strategically on Terenure Roadway North, this delightful Indian restaurant offers a wide variety of traditional Indian food along with modern specials. This location specialized in the yummy chicken jalfrezi - chunks of hen meat stewed with onions, scallions, peppers, and a mix of Indian herbs all cooked in standard Indian tandoor. The restaurant is dedicated to using fresh neighbourhood fruit and vegetables to make certain that the foods that they offered to their consumers are of the finest. The restaurant's courteous team and unwinding ambience are set to make your eating experience an extraordinary one.
Location: 94 Terenure Rd N, Terenure, Dublin 6W, Co. Dublin, Ireland
Timings: Tue - Wed: 5:30pm - 9:30pm; Thu - Sat: 5:30pm - 10pm; Sun: 4pm - 9:30pm (closed on Mon)
8. Rasam Restaurant
If you're searching for an Indian restaurant with a setting that makes you feel like you're already in India, Rasam is the area to go. Not just that, the food they serve is top-notch as well as their service is likewise admirable. Several of their food that obtain high favourable evaluations are slow-cooked lamb shank with coriander, tomato masala served with basmati rice and buttered nan bread, and murgh tikka - hen pieces are soaked in an experienced yoghurt sauce, offered kebab-style. For dessert, attempt their mango and also passion fruit cheesecake, which is said to be heavenly.
Location: 18-19 Glasthule Rd, Dún Laoghaire, Dublin, A96 H2N1, Ireland
Timings: Mon - Sat: 5:30pm - 10:30pm; Sun: 5pm - 10pm
7. Pickle Restaurant
There are many Indian restaurants in Dublin, but when it comes to an Indian restaurant that offers delicious authentic Indian food old-school design, Pickle is the pick of the bunch! Do you not know what to get from their menu? Give these a shot: samosa chaat, various variants of kebab (shellfish, poultry lamb, and aloo), and duck tikka - a plate of tender spiced duck meat served skillfully. If you feel like having a full course meal, order a dinner veg thali which permits you to attempt many different tasteful curries.
Location: 43 Camden Street Lower, Saint Kevin's, Dublin 2, D02 N998, Ireland
Timings: Tue, Sat: 5pm - 10pm; Wed - Fri: 12pm - 2:15pm, 5pm - 10pm; Sun: 3pm - 9:30pm (closed on Mon)
6. Spice of India
The moment you get in Spice of India, you would have the ability to tell that this restaurant will give you an eating experience of a lifetime - tickling your taste with a substantial selection of Indian cuisine. Some of their foods that have gotten positive reviews from restaurants are chicken tikka masala and lamb korma. A selection of red wines, beers, and ciders is also readily available to accompany your dining experience. Luckily, for those who choose to appreciate their dishes in your home, this restaurant likewise supplies a residence distribution solution. Seasoning of India has three Dublin locations: Clonsilla, South William Street, and Swords. Have a look at their internet site for the addresses of other places.
Location: Weavers Row, Clonsilla, Dublin-15, Ireland
Timings: Mon - Thu: 4pm - 11pm; Fri - Sat: 4pm - 11:30pm; Sun: 1pm - 4pm
5. Aleena Restaurant
A fan of hot Indian food? After that, a visit to Aleena Restaurant should be on your shopping list. Drink white wine and appreciate delicious baltis and tandoori meals made of the choicest meat, poultry, and fish and shellfish. For restaurants that do not consume meat either milk products, the restaurant also provides Indian vegan dishes. This restaurant, which was established in 1993, also offers halal food in the form of halal curries. What makes this restaurant also better is the food below is valued rather reasonably, and also, they remain open until dawn. If you do not seem like eating in, you can have your meal as a takeaway or have it provided.
Location: 3 Temple Ln S, Dublin 2, D02 TY36, Ireland
Timings: Mon - Wed: 5pm - 11:30pm; Thu - Sun: 5pm - 4:30am
4. Bombay Pantry
The winner of Wonderful Taste Honors in the UK for six strong years, no surprise this restaurant has seven branches all over Dublin. The majority of the menu is fairly comparable to the various other Indian restaurants in Dublin; nevertheless, they placed special attention on their vegan menu. Their vegetarian meals such as pleasant potato dahl and chana masala are done deliciously - fresh, authentic, and rupturing with tastes. They often offer special offers which enable diners to obtain yummy dishes on a budget plan. In addition to everything, what's so wonderful about this restaurant is they even supply nutritional information published for every dish! For the address of each of their areas, see their site.
Location: Unit 6, Rathbourne Village, Ashtown, Dublin 15 D15 WK18
Timings: Sun - Thu: 4pm - 10pm; Fri - Sat: 3pm - 10:30pm
3. Konkan Restaurant
Dubbed as one of the most effective Indian restaurants around, Konkan in Clanbrassil Road, which was developed in 2004, has handled to safeguard a variety of distinguished cooking awards. Their menu consists of a variety of Indian foods that are popular in the nation in addition to their specialized, which mainly are South Indian meals. The restaurant, which has cosy and kicked back ambience, prides itself in its dedication to offer fresh, quality Indian food at sensible costs. To ensure that their consumers are offered only the very best foods, they prepare their active ingredients such as paneer, garam masalas, and yoghurt in the house.
Location: 46 Clanbrassil Street Upper, near Harold's Cross, Dublin, D08 RF88, Ireland
Timings: Mon, Wed - Sun: 5pm - 11pm (closed on Tue)
2. Ravi's Kitchen
If appealing Indian food with reasonable rates is your thing, then Ravi's Cooking area is where you need to be heading. They have a two-course lunch menu that is reasonably valued and also kindly plated. Chilli haldi prawn, sagwala, paneer, and garlic naan are several of their must-order dishes. No requirement to bother with difficult dining decorum below, Ravi's Kitchen area is a simple and unfussy restaurant where all you have to do is sit back and take pleasure in the great food and the friendly ambience.
Location: 174 Pembroke Road, Ballsbridge, D04 N4X5, Ireland
Timings: Mon - Fri: 12pm - 2:30pm, 5:30pm - 10:30pm; Sat - Sun: 5:30pm - 10:30pm
1. Ananda
A haven in the middle of Dublin's busy mall is what Ananda restaurant is. The area, which has functioned since 2008, was founded by the very first Indian cook to be warranted with a Michelin Celebrity - Atul Kochar. Once you enter this restaurant, you'll find yourself in a space with lively colours which will instantly advise you of India. Obtain their pan-fried potato galettes with tamarind and raisin, or fried tiger prawns spiced with chilli and also garlic that includes cucumber yoghurt - both are stated to be finger-licking excellent. For the main course, try tandoori murgh Amritsari - a portion of delicious chicken meat offered with flavorful masala sauce. All keys are served together with naan bread as well as pulao rice.
Location: Cinema Building, Sandyford Road, Dundrum Town Centre, Dundrum, Dublin, D16 VK54, Ireland
Timings: Mon - Thu: 5:30pm - 10:45pm; Fri - Sat: 12:30pm - 2:30pm, 5:30pm - 10:45pm; Sun: 1pm - 2:45pm, 5:30pm - 9:30pm


text:
Experience Dual Track Coaching
Learn The Theory
Practice The Skill
Achieve Mastery
Get in touch with us today.
Hot Skills For Organizations & Individuals
Managers are abundant.- leaders are scarce. That makes leaders more valuable.
You don't learn skills by listening to a lecture or watching a slide deck. .
If you are on influencer, you have a business module you can monetize.
In the Information Age, your SUSTAINABLE competitive advantage boils down to how quickly you can learn and adapt. Strange though it may seem, we are expected to be "life-long learners." But the educational system doesn't bother to teach you how to learn either expertise or skills. You are just expected to know.
Bio: Murray Johannsen
Your Executive Coach
The Reason I Got Interested in Skill Building
A Sample Mastery Practice
Hi there! I'm Murray Johannsen, founder of Legacee and the Legacee Academy.
My journey into this field was sparked by the realization that our educational system often fails to teach us how to learn and master skills (see My Great Awkening).
And yet, these are the very capabilities that organizations are eagerly seeking. This led me down the path of creating a system that fills this gap.
It's built on foundation of a B.S. in Pharmacy and a MBA from the University of Iowa, along with an M.A. in Psychology from Harvard University,
So I feel well-equipped to develop engaging and impactful content for a series of online full-classes and short courses for teaching and coaching.
And the best part? I'm constantly learning too, testing these programs by teaching them myself.
As an adjunct professor, I specialize in more conventional but highly vital subjects like:
— Digital marketing,
— Entrepreneurship,
— Psychology, and of course
— Leadership.
My approach is driven by my belief that our education system should teach us more about learning and skill mastery - critical areas that organizations actively seek.
In my many years of experience, I've had the privilege of training and coaching a diverse range of individuals, from managers and executives to employees in transformational philosophies such as Total Quality Management.
I'm passionate about turning potential into proficiency.
So, if you're looking for a coach with a wealth of practical and academic experience, coupled with a passion for transforming potential into mastery, do connect.
Let's explore how we can work together to help you thrive in your entrepreneurial or executive journey.
Copyright © 2017-2023 by Murray Johannsen. All Rights Reserved.


text:
Algoma Steel Group Inc. (ASTL)
NASDAQ: ASTL · IEX Real-Time Price · USD
-0.20 (-2.47%)
Mar 1, 2024, 4:00 PM EST - Market closed
Algoma Steel Group Income Statement
Financials in millions CAD. Fiscal year is April - March.
Millions CAD. Fiscal year is Apr - Mar.
Source: Financials are provided by Nasdaq Data Link and sourced from audited reports submitted to the Securities and Exchange Commission (SEC).


text:
In addition, the Romans used the new thermal waters to alleviate their suffering from rheumatism, arthritis, and overindulgence in food and drink. Spa therapies have existed for the rationale that classical times when taking tub with water was consider
10 Magnificence Treatments & Spas Value Travelling To South Korea For
Published on :According to Amy Frigulietti, Wellesley's Assistant Executive Director, the city has been addressing roadblocks to filling empty storefronts inside its jurisdiction, similar to licensing and permitting. "We're thrilled that momentum from the storefront
Island Magnificence Spa Getaway 2022
Published on :There is a choice of spa-related resorts and bathing services continuing the town's tradition of welcoming guests to partake in therapeutic remedies. One of the extra luxurious is Carolus Thermen resort – one m
Connecting Magnificence And Wellness, Nyc Medical Spa Focuses On Multi
Published on :I stopped doing the massages once I went to university as a end result of I was like who has time for that? And when I'd go to residence on the weekends, it will bother my mother so much. She'd be like, "Your hair n
The Top 3 Merchandise Our Magnificence Editors Beloved Using In April
Published on :This will create return prospects who, over time, will form a dependable customer base. Whatever it might be, attempt to make it a focal point of your identity and grow your small business from there. Growing your corporation in a distinct segment mark
Finnish Pure Magnificence And Wellness Heritage
Published on :We investigated free salon and spa scheduling techniques to find out. Increasing the number of purchasers who buy retail supplies you with the chance to earn more income from each appointment. When a future ap
Spa Sibo Magnificence Salon
Published on :There's no doubt that Instagram has allowed us to travel just about to destinations we've only ever dreamed of. We can merely signal on and jet off to new locations, expertise new cultures, try new foods, and
05 Magnificence Tradition & Spa
Published on :I became an instructor of superior methods which lead me to at present. I supply designer peels, resurfacers, microdermabrasion, microcurrent and waxing. My therapies are bio-targeted to every person
Embrace Your Magnificence And Well Being At Epiphany Salon
Published on :Some are bricks-and-mortar establishments that present online studying as a half of their prolonged university programs whereas others solely offer on-line courses. It seems that there's one thing intrinsically human in seeking a reprieve from daily li
Actual Magnificence Tradition Spa In Dallas, Tx
Published on :These resorts supplied swimming, fishing, hunting, and horseback driving in addition to facilities for bathing. The Virginia resorts, significantly White Sulphur Springs, proved popular before and after the Civil War. After the Civil War, spa vacations
Your Magnificence Gift Guide For Moms Day 2022
Published on :Treatments typically conclude within the spa's Sultana Lounge where visitors chill out on beds of velvet and silk, indulging in tea and sweet cake. The answers provided under are based on solutions beforehand given b
Toni Braxton Expands Magnificence Empire With New Distribution Deal
Published on :When Franklin Delano Roosevelt was governor of New York, he pushed for a European kind of spa development at Saratoga. The architects for the new advanced spent two years learning the technical aspects of bathing in Europe. Completed in 1933, the devel
The Most Effective Winter Magnificence Escapes To Comb You Off Your Feet
Published on :Keep stations litter free by having loads of salon storage for products and tools. Clearing a station after each shopper is instrumental in allowing the shopper that follows to have a unique experience that's all
Embrace Your Magnificence And Nicely Being At Epiphany Salon
Published on :Marketing is an essential course of for each enterprise, but sure techniques work higher for some companies than others. When opening a neighborhood salon, you'll need to give consideration to turning into part of your community. Since you're a brick-a
Magnificence Culture Med Spa
Published on :@SwivelBeauty has created an app to alleviate the stress for women of colour to search out reputable salons with awesome experiences. However, as stylists inside this realm, it's our responsibility to begin a shift within the salon experience provided
Spa Culture North Bend Magnificence Salon, North Bend Oregon Magnificence Salon, Hair, Nails, Facials, Waxing
Published on :Tell people, "let's go to Elegushi beach" and the response can be "Ah! Oya, turn up", but inform them "let's go for a massage", they'll start giving you tales that touch ("times are hard", "you know say I no g
10 Magnificence Therapies & Spas Price Travelling To South Korea For
Published on :They are the first resorts in Mexico with presence in its listing. Our business is true in the middle of downtown BRF & we take great delight in serving our growing group and folks. River Haven is a full service hair salon that has been round for over
Connecting Magnificence And Wellness, Nyc Medical Spa Focuses On Multi
Published on :UB City Bangalore UB City Bangalore A getaway in the coronary heart of UB City with therapy rooms designed to complement the genuine spa expertise. Seaview Xiamen Seaview Xiamen Nestled on the picturesque seaside line, the spa greets spa aficionados wi
Magnificence Culture Medspa
Published on :French Lick specialised in treating weight problems and constipation via a mix of bathing and consuming the water and exercising. Hot Springs, Virginia, specialized in digestive ailments and heart ailments, and White Sulphur Springs, Virginia, handled
Magnificence Tradition Spa Centre Excessive Resolution Inventory Images And Images
Published on :In her spare time she enjoys coaching soccer, hiking, skiing, travelling, good food and spending quality time together with her household. Her approach to pores and skin is all about progress, not perfection, and sustaining good skin health whereas aim
The Highest 3 Merchandise Our Magnificence Editors Liked Utilizing In April
Published on :Like all Korean bathhouses, the hot bathtub and steam sauna are separated by gender to sweat it out in the nude. For those not able to go bare, you have the choice of donning a swimsuit. Rest assured that Karma is committed to cleanliness in all we do.
Finnish Pure Magnificence And Wellness Heritage
Published on :This majestic hotel presents some of London's most unusual rooftops and houses several one-of-a-kind suites. It is also home to one of the city's most well-known and opulent afternoon tea festivities, which are held
Magnificence Culture Spa Centre Excessive Decision Stock Pictures And Images
Published on :Gwyneth Paltrow Is in Your KitchenGoop Kitchen brings its elevated vibes to mealtime with takeout pizza, chicken salad, and different very match meals. Then, Her Life Fell Apart.Best-selling author Kelly Williams Brown reflects on coining that now drea
Tricoci College Of Magnificence Tradition
Published on :The pool space partitions boast unique engravings from hundreds of years in the past, adding a whimsical sense of magic that helps you dream, relax, and unwind while you swim. Don't neglect to indulge within the se
The Top 3 Products Our Magnificence Editors Liked Utilizing In April
Published on :Ushvani was as soon as voted the U.K.'s best day spa by Tatler and Condé Nast Traveller. It's simple to see why once you enter the oasis within the centre of Chelsea. With ideas based mostly on historic
Finnish Natural Magnificence And Wellness Heritage
Published on :I write about conscious luxury, specializing in travel, well-being & artwork. For our money although we'd opt for a Laz-y Hot Tub, as they've a superior jet-stream and make for a greater 'massage' reall
Spa Sibo Magnificence Salon
Published on :However, nights are white throughout the country for many of the summer. There are several forms of saunas, the commonest being electric, wood-heated and smoke saunas. Coloured lights, fragrant fragrances and stress-free music don't have anything to do
05 Magnificence Tradition & Spa
Published on :With some flexibility, you'll get to your destination often sooner and cheaper than other means. On the website or app, enter your departure and arrival points and your date of journey, sift through the profiles of drivers who've posted pre-planned dri
Embrace Your Magnificence And Properly Being At Epiphany Salon
Published on :Health, wellness, nature, artwork, and culinary delights are the focal point of this world-class Swiss resort. Book a superb room this Mother's Day for a view that overlooks town of Zurich, the lake, and the Alps. The
Actual Magnificence Culture Spa In Dallas, Tx
Published on :And, in fact, every is well-equipped with facilities like luxurious showers and relaxation rooms that will tempt you to stay for hours. Offering an array of way of life decisions and encompassing whole well-being, Willow Stream Spa at Fairmont Quasar I
Toni Braxton Expands Magnificence Empire With New Distribution Deal
Published on :For occasion Kotiharju Sauna within the Kallio district is the final remaining wood-heated native sauna in the metropolis the place you might also enjoy a massage or try the ancient treatment of cupping. If you need to enjoy an actual Finnish wooden sa
05 Magnificence Tradition & Spa
Published on :Giving your physique higher blood circulation and relieving you of constant body ache? Things like this which improve the standard of one's life ought to NEVER be considered as expensive. We solely imagine in
Leslie Mann And Maude Apatow Nonetheless Share Magnificence Merchandise
Published on :Quality is also supposed by all of us, by our partners and consumers as high quality of life. Specializing in multi-cultural skincare, Gibson spoke of the number of ways she cares for women and men with dark pores and
Angel's Touch Magnificence Spa In Lethbridge, Alberta
Published on :Color palette is predicated on cold, "Baltic" colors which support the view outside. Textile fabric and wood components are there to mitigate shades of grey. The core of this idea is a mirror-made dice which subsequently became the authentic function o
Contact True Culture Barber Lounge, Magnificence & Spa In Acworth, Georgia
Published on :If we could reside completely inside any Chanel assortment, it would almost actually be cruise. Overnight in Monte Carlo, creative director Virginie Viard presented Cruise '23, a set that paid tribute to
Spa Culture North Bend Magnificence Salon, North Bend Oregon Magnificence Salon, Hair, Nails, Facials, Waxing
Published on :In Great Britain, ancient legend credited early Celtic kings with the invention of the hot springs in Bath, England. The hair and wonder industry is so saturated with salons that it truly is not sufficient to simply perform good high quality services.


text:
- This topic has 2 replies, 2 voices, and was last updated 4 years, 7 months ago by .
Viewing 3 posts - 1 through 3 (of 3 total)
Viewing 3 posts - 1 through 3 (of 3 total)
- You must be logged in to reply to this topic.
Home › Forum › SOFA › Programming with SOFA › [SOLVED] Changing object positions after every reset
Tagged: 64_bits, Linux_ubuntu, SOFA_1706
I am trying to reinitialize object positions in my scene by changing translation parameter. But the scene gets created in createScene() function and then after reset always starts at the same positions. I want to be able to change positions of object randomly after every reset, any suggestions?
Scene File: link
Thank you,
Hello, Rishabh!
The MechanicalObject class has functions that apply the *initial* transform to an object: applyTranslation and applyRotation. Combined with the various getObject functions from the BaseObject class, you should be able to enumerate all MechanicalObjects in your scene and customize their initial positions before you step the simulation for the first time.
With best regards,
The forum has been moved to GitHub Discussions.
Old topics and replies are kept here as an archive.


text:
EA explains why FIFA Ultimate Team transfer prices are now platform specific
EA has posted a blog entry explaining its FIFA Ultimate Team transfer market price ranges which now include platform-specific pricing in order to best the coin sellers.
EA said it has implemented the changes to FUT over the last several weeks because it should be "fair and fun for every player," ultimately coming to the decision that the Transfer Market no longer needs to be accessible via the web or through the Companion App.
"We want to keep the game fair and secure for everyone, and ensure a level playing field for all FUT fans," said the firm on its blog. "To accomplish this we have to root out the activities of coin farmers and cheaters who are harming your experience with the game.
"Since implementing these changes, we're seeing positive signs of restoring balance to the FUT economy and mitigating some of the negative effects of exploitation within the game."
Those who exploit system using bots and phishing scams generate coins which flood the FUT economy. The fake in-game currency the drives up the cost of players on the Transfer Market, damaging the economy and making top players unattainable for the vast majority of players.
EA explained the actions it is taking and said it is considering including new changes to Price Ranges, as well as the status of the web and Companion Apps.
One of the actions taken was a change to Price Ranges which it said helps "restrict illegitimate coin transfers on the FUT Transfer Market." It also balances player item prices to better reflect the current marketplace.
EA said finding the "right balance will take some time, but we have a team dedicated to finding that balance."
Another change which the firm says will provide better trading balance and fairness is varying Price Ranges per platform which "reflect the values and dynamics in those unique economies."
Access to the Transfer Market via the web and Companion Apps has been pulled since the February 13 update as the Apps became "one of the main methods for coin sellers and farmers" to move counterfeit coins through the FUT economy.
The Transfer Market will be brought back into the Apps and online, but only once the team as made a more stable and safe experience.
EA provided a list on the price differences between the PC, PS4 and Xbox One Transfer Markets in the blog post. If you are a FIFA player, you should really give it a look. There is also a Q&A available.
FUT is in its sixth year and those who play are being handed one free pack every day, and is offering players a chance at receiving tradable In-Forms just for playing in the FUT Birthday Cup one time - or morr.


text:
A major in applied software engineering requires 61 - 64 credit hours of coursework, depending on placement. Students in applied software engineering begin their study with a basic core of informatics and information technology coursework, then move on to a chain of courses designed to build their software development skills and prepare them to implement user centered full stack applications. The program includes a set of electives from a variety of degrees offered by the computer science department. The major culminates in a team-project capstone course (ASE 485).
The ASE degree differs from a degree in Computer Science in its focus on software engineering and the theory and skills needed to be successful in this field. While the CS degree is the study of computation, the ASE degree focuses on applying theory and practice for Software Engineering.
The ASE classes are offered during daytime and in the evening, and in multiple formats (face-to-face, online, and hybrid). This allows students to customize your schedule in a format that works for them and their life.
Special graduation requirements: Students must earn a grade of C- or better in each course that counts as part of a major or minor for all programs offered by the department.
Four-Year Plan: Click here to view the Four-Year Plan for this specific program. To find out more about Four-Year Plans, visit the Policies and Procedures page.
♦ Indicates prerequisite
Any advanced 3-hour course (3xx/4xx) not listed above with an ASE, CIT, CSC, CYS or DSC designator.
Take one of the following classes. If the course taken is for 3 credit hours and is one of the ASE courses, it can count for the elective requirement listed above.


text:
Stacy Charles has a close relationship with Kathy and Steve Elkins, the owners of the Massachusetts yarn store WEBS, so when it came time to look at a new business model for his yarn company, Tahki Stacy Charles, he knew exactly who to approach.
"The relationship I've had with Kathy and Steve for many years has allowed us to speak openly about each other's businesses and the challenges that were in front of everyone," Charles said in an interview a few days after the announcement that WEBS would be acquiring both Tahki Stacy Charles and String Yarns, a New York City shop that Charles purchased in 2015.
With their online presence through yarn.com, the Elkins have the technology and marketing resources to support Tahki Stacy Charles into the future, Charles said.
"I wanted to focus on less rather than more," Charles explained of the company's shift.
WEBS also has product development experience via the store's Valley Yarn line, which is sourced from a variety of mills around the country.
"I think there's a lot that we can add from a product development and also a marketing support standpoint," said Kathy Elkins during a recent conversation about the acquisition.
WEBS retail shop. Image courtesy Stacey Trock.
Both Charles and the Elkins sent emails to wholesale and retail customers announcing the acquisition, which comes at a time of tight margins and a change in consumer expectations.
"There is an expectation that Amazon has established, that people are like 'Well, why is it going to take three days to get to me?'" Elkins said. "There are pressures not necessarily in our industry, but outside our industry."
While a retailer owning a wholesaler is a unique shift in the fiber industry, it's not so new in the retail world, Charles and Elkins said.
Charles, who designs and manufactures both luxury and affordable yarns under the Stacy Charles Fine Yarns and Tahki Yarns labels — he merged with Tahki Yarns in 2001, and he and Tahki founder Diane Friedman co-own the company — compares the business model to designer fashion brands, such as Tory Burch and Michael Kors, which sell both wholesale to department stores and direct to the consumer.
"It's a definite shift, but it's nothing that should be so monumental in today's day and age," Charles said.
Many smaller companies in the yarn industry, particularly independent dyers, sell to both consumers and yarn shops.
"Bigger wholesalers are held to a different standard," Elkins explained.
In fact, Charles had already upended the traditional model by purchasing String, developing a line of yarns called String@ that are sold wholesale to 15 shops around the country.
As part of the acquisition, which takes effect on April 1, Charles will stay on as brand and creative manager for Tahki Yarns, Stacy Charles Fine Yarns and String, and will also take on a retailer sales management role, running both the wholesale divisions of the labels and the String retail store.
Tahki Stacy Charles yarns will ship out of WEBS' warehouse in Easthampton, Mass. The company has a team of three that work remotely who will remain on staff.
Elkins said that while the overall reaction from WEBS' wholesale partners has been supportive and congratulatory, some of WEBS' and Charles's customers have expressed concern about the change, as they see WEBS as a competitor.
"I fully understand that point of view," Elkins said. "But I would hate to see any retailer in what is a very difficult market abandon product that is working for them."
Even with WEBS' success in online retail, Charles doesn't see WEBS and local yarn shops as competitors, as each has its own community.
Elkins echoes this sentiment, noting that String Yarns and WEBS are very different stores and will remain separate. While the Elkins will help String with its technology, first computerizing its inventory, which is currently done manually, the two shops will remain separate.
"String has a finisher on staff because that is what their customer expects," Elkins said. "WEBS doesn't employ a finisher and we encourage our customers to take our Fearless Finishing class. Neither option is right or wrong, just different based on the customer needs and expectations."
Lisa Chamoff is a freelance journalist in the New York Metro area who specializes in home design, real estate, and healthcare. When she's not writing, or knitting shawls and sweaters, Lisa runs Indie Untangled (www.indieuntangled.com), a marketplace and blog that promotes the work of yarn dyers, pattern designers, and crafters of knitting-related accessories.


text:
Swift provides a Set data structure, which is a collection type that stores unique values of the same type in an unordered fashion. Set operations allow you to manipulate these sets in various ways, such as checking for membership, adding and removing elements, and performing operations like union, intersection, and subtraction.
In this article, we'll explore some of the top tips and tricks for working with sets in Swift.
- Creating a Set
To create a new set in Swift, you can use the Set initializer or simply declare an empty set with the type annotation.
var mySet = Set<Int>() var anotherSet: Set<String> = []
- Checking for Membership
You can check if an element is a member of a set by using the contains method.
let mySet: Set = [1, 2, 3, 4] if mySet.contains(3) { print("3 is in the set") }
- Adding and Removing Elements
To add a new element to a set, you can use the insert method. To remove an element, you can use the remove method.
var mySet: Set = [1, 2, 3] mySet.insert(4) mySet.remove(2)
- Set Operations
You can perform various set operations on Swift sets, such as union, intersection, and subtraction.
let setA: Set = [1, 2, 3, 4] let setB: Set = [3, 4, 5, 6] let unionSet = setA.union(setB) let intersectionSet = setA.intersection(setB) let subtractionSet = setA.subtracting(setB)
- Set Algebra
Swift sets support set algebra, which allows you to perform operations like union, intersection, and subtraction with multiple sets.
let setA: Set = [1, 2, 3, 4] let setB: Set = [3, 4, 5, 6] let setC: Set = [1, 3, 5, 7] let algebraSet = setA.union(setB).intersection(setC)
In conclusion, Swift sets are a powerful tool for working with collections of unique values. By using the tips and tricks outlined in this article, you can take full advantage of set operations in Swift and simplify your code.


text:
Could India make history with a back-to-back win? Or could Bahrain be the first Middle Eastern country to take home a crown?
The Miss Universe 2022 pageant may be postponed to January next year, but pageant fans and followers are already picking their favourites, speculating on who they think could win the competition.
Scroll through the gallery above to see Miss Universe beauty queens crowned in 2022 so far
In their latest ranking, YouTube channel Crown Sisters, which aims to deliver "fresh and fair" pageant news, places Miss India Divita Rai on top. Miss Bahrain, Evlin Khalifa is at No 16, while Miss Universe Puerto Rico, Ashley Ann Carino (No 2) and Miss Philippines Celeste Cortesi (No 5) also rank high.
Sash Factor, another pageant site, places Rai at No 10, while Khalifa comes in at No 12.
Meanwhile, Missotopia, also a pageant fan site, has India at No 9 and Bahrain at No 20.
Following Harnaaz Sandhu's win of the Miss Universe 2021, all eyes are on Rai, 23, an architect from Karnataka in South India, who won the Indian pageant in August. If Rai wins the crown, she will make history as the second country to win a crown back-to-back.
Only Venezuela has achieved that feat before, winning in 2008 and 2009. The pageant-mad country has won the Miss Universe title a total of seven times, second only to the US, which has won eight.
"We have a system placed especially for Miss Universe in observing and analysing the probability for a contestant's placement based on the criteria on what the Miss Universe Organisation is looking for," Brix Vera from Sash Factor tells The National.
Miss Bahrain Khalifa has "huge potential to break and make history", she says.
"During Miss Universe Bahrain 2022, we noticed Evlin Khalifa as the strongest on her batch, and with the proper training and guidance, her placement at Miss Universe 2022 is not impossible. We also have taken note on her modelling career as well as her charitable back stories."
While by no means official, these rankings are not to be taken lightly, says Josh Yugen, the national director for Miss Universe Bahrain.
"These pageant bloggers serve as the soul of the pageant industry as they are true fans of the competition," he says. "They have been following every pageant, from the day a contestant applies for their national pageant until they compete and represent their countries. Most of them are well-informed and have every information about past winners, their looks and even the answers of each candidate.
"It is safe to say that pageant bloggers are the unofficial jury of the pageants as they have in-depth information and analysis about the competition. And most of them can accurately predict the winners."
Yugen, who is also the chief executive of Yugen Group based out of Dubai, says he's excited to help improve representation of women from the Gulf on a global stage.
"Miss Universe Bahrain is the first of its kind to represent the region and this is part of what we are fighting for — representation," he tells The National.
"If you look into Hollywood movies, Disney films and even international Netflix shows or fashion shows, there is not enough representation of women in the Gulf, most are stereotypes that they are covered, intimidating or unapproachable.
"But the women in the Gulf have their own charm, their own beauty and their own personalities that contribute to the modernisation of the society. They are fighters, innovators and most importantly kind-hearted women who also deserve big representation on the Miss Universe stage."
New Orleans in Louisiana will play host to the Miss Universe 2022 pageant, to be held on January 14.
While a number of countries are yet to name their representatives, 70 contestants have already been chosen through national competitions.


text:
Grapes are delicious fruits that are absolutely bursting with juiciness. Their mildly sweet flavor results in grapes being quite a versatile ingredient since you can pair them well with a variety of flavors.
Not to mention, grapes work well in both savory and sweet dishes. Therefore, you can use them in anything from cheesecakes to salads.
Whether you prefer green or red grapes, these fresh fruits are incredibly popular. If you are interested in using this ingredient, here are 23 incredible recipes for you to try.
1. Grape Salad
Salads have developed a reputation for being bland. Luckily, this grape salad recipe is full of flavors. It works fantastically as a side dish. Or, if you are an avid grape lover, it will be a magnificent main meal.
A reason to love this salad is that it's super simple to make. It only requires 10 minutes of preparation time, so you won't need to spend ages working on this salad.
This dish has a unique texture, from the creaminess of the cream cheese to the crunchiness of the chopped pecans.
2. Smashed Sweet Potato With Roasted Grapes
This smashed sweet potato and roasted grapes recipe is perfect for a warm and inviting dinner. The result is a potato dish that's perfectly crispy on the outside and soft on the inside.
Sweet potatoes are loaded with flavor. This recipe preferably seasons the potatoes, using goat's cheese to balance the sweetness.
Thanks to the use of thyme, salt, and pepper, the sweet potatoes are far from dull. The roasted potatoes work astonishingly well with the rest of the ingredients, complementing the sweetness of the potatoes.
3. Grape Cheesecake Tart
If you are searching for a sweet dish, this delicious grape cheesecake tart is a sensational solution. The butter pie crust is covered with a creamy cheesecake filling.
This filling contains chunks of grape, providing a pleasant burst of juiciness in every bite. One of the reasons why we love this dish is that you can use whatever color grape you prefer.
Personally, we prefer using red or purple grapes as they have a more visually appealing aesthetic, but the choice is entirely up to you!
4. Blueberry, Grape, And Banana Smoothie
In this recipe, grapes are combined with blueberries and bananas to create a tart smoothie that's filled with fruity goodness. You can make this recipe in just 5 minutes, making it ideal for breakfasts or whenever you want a refreshing drink.
This sweet smoothie won't require any sugar, as the natural sweetness of the fruits provides enough flavor. Therefore, this remains a healthy treat. Simply add a little bit of honey to instill this beverage with a sweet and floral taste.
5. Red Rice With Grapes And Pistachios
This red rice recipe has complex flavors that few other dishes manage to achieve. It manages to combine red rice, onion, grapes, and pistachios. This creates a unique and sophisticated flavor.
The use of seasonings is also superb, with ingredients such as cardamom and black pepper tying the ingredients together incredibly.
Perhaps what we love most about this recipe is the broad array of textures. The softness of the rice is fabulously contrasted with the crunchiness of the pistachios and the fleshy grapes.
6. Ruby Grape Pie
When you think of fruit pie, you likely automatically turn to apple pie. In our opinion, grape pie is an underrated delicacy. This delectable recipe proves just how lovely this dish can be.
The use of lemon juice and zest enhances the flavor of these grapes, ensuring that they have a tart and slightly sour taste. This fantastic filling is contained within a butter pie crust.
We especially like the idea of carving the word "GRAPE" into the top of the pie crust, as this allows you to see the precious ruby grape filling.
7. Shrimp With Grape Salsa And Couscous
If you are looking for an easy yet tasty weekday meal, this shrimp with grape salsa and couscous is an excellent choice. Flavored with cilantro and lime juice, the grape salsa is perfectly refreshing.
Combined with the yogurt and dill sauce, the shrimp is loaded with fantastic flavors. Pan fried, this salty shrimp pairs with the filling couscous terrifically.
Despite all of the different components that combine to form this dish, it will be ready in just 30 minutes.
So you won't need to spend ages cooking in the kitchen. Instead, you can spend your time enjoying this tantalizing dish.
8. Chocolate Covered Grapes
Do you love chocolate? Most people find it irresistible. This chocolate-covered grape recipe is ideal for chocolate lovers. This is a ridiculously simple recipe.
So even if you aren't much of a baker, you can easily make these grapes. This is also a good opportunity to get kids involved with cooking.
These chocolate-covered grapes make for magnificent snacks. They're also customizable since you can coat them with whatever you want. This includes peanuts and toasted coconut. Plus, you can get creative with your chocolatey decorations.
9. Turkey Salad With Grapes
Turkey salad is a terrific way of using up your Thanksgiving or Christmas leftovers. We particularly love this turkey salad recipe because it combines the meat with juicy grapes.
The addition of lemon juice, pecans, and celery to this salad is inspired. The result is a luscious creamy salad. This turkey salad is a perfect sandwich filling.
So if you've become bored of the same old sandwiches, simply spread this succulent salad in between two slices of bread. This is an easy yet tasty lunchtime treat.
10. Grape Pizza
If you enjoy pineapple pizza, you'll likely adore this amazing grape pizza recipe. Though the idea of adding grapes to a pizza may sound a little odd to some, trust us, it works wonderfully.
One of the reasons why we appreciate this recipe is because it's super simple to make. You don't have to make your own pizza dough. Instead, you can simply use a store-bought variety.
Plus, the tanginess of the ricotta, mozzarella, and parmesan cheese harmonize with the grapes beautifully, especially when seasoned with rosemary and chili flakes.
11. Oatmeal Porridge With Grapes And Apricot
Do you like to start the day with a healthy breakfast? If so, you should make this outstanding oatmeal porridge with grapes and apricot.
These fantastic fruits combine excellently with the porridge, creating a warm and sweet dish that's incredibly appetizing on chilly mornings.
As this recipe proves, oatmeal doesn't always have to be dull. This is especially true when it has been enhanced with cinnamon and maple syrup. Be generous with the maple syrup if you prefer sweeter oatmeal.
12. Goat Cheese Pasta With Chicken And Grapes
Goat's cheese is something of an acquired taste. Yet, if you like this tart and creamy cheese you're going to fall in love with this fantastic recipe.
Grapes and goat's cheese is a popular combination for good reason. The flavors just work so well together, creating a tangy taste that few other ingredients can provide.
Every element of this dish is loaded with flavors, from the balsamic chicken to the red pepper flakes infused grapes. If you crave a sophisticated dinner recipe, this cheesy chicken and grape pasta is an outstanding option.
13. Grape Ice Cream
Grape ice cream is a rare delicacy. Though it may not be as popular as other flavors, we believe that you're going to love this grape ice cream recipe. It has an attractive pale purple color scheme.
It's incredibly refreshing on warm days, so you may want to make this great grape ice cream recipe over the summer. It's tons of fun to make and homemade ice cream tastes so much better than store-bought versions.
Simply serve with some fresh fruits for an effective dessert. Alternatively, you can serve this alongside a main dish, such as a warm fudge brownie.
14. Thai Red Curry With Grapes
Do you enjoy spicy food? If yes, this Thai Red Curry recipe is bound to become one of your favorites! Thanks to its spicy kick, this curry is sure to warm you up. Luckily, the refreshing grapes provide a coolness, thus taking the edge off the spice.
This fragrant curry is overflowing with flavors, thanks to the inclusion of seasonings like ginger, lemongrass, coconut milk, and garlic.
If you want an inventive way of using grapes, this Thai Red Curry recipe is the perfect option. Simply serve with some rice for a delectable dinner.
15. Grape Jam
Perfect for spreading on scones and crackers, this exquisite jam recipe is one of our favorites. The way that it brings out the mellow flavor of the grapes is remarkable, as many other jam recipes instead resort to adding copious quantities of sugar.
This is partially due to the use of lemon juice, which brings out the tart taste of the grapes. Additionally, all you will need for this recipe are 3 ingredients.
These are sugar, grapes, and lemon juice. So this simple recipe won't cost much money to make.
16. Pavlova With Roasted Pears And Grapes
This spiced pavlova recipe is a beautiful and eye-catching dessert that is bound to impress anyone that tries it. It has a warm and earthy taste that makes it perfect for fall or winter.
The pavlova has a crunchy exterior with a soft and chewy interior. This layer has been ideally seasoned with cardamom for a nutty taste. It has then been topped with a thick crème fraîche, providing the dish with a luxurious creaminess.
The final layer of this impressive dessert is a layer of roasted pears and grapes, adding some delightful natural sweetness to the dish.
17. Concord Grape Crisp With Peanut Butter Crumble
Concord grapes provide the perfect balance between sweet and tart. This recipe is one of the best uses of this glorious grape variety that we have ever seen.
This crumble recipe is an ingenious adaptation of the peanut butter and jelly sandwich. It features a layer of concord and is topped with peanut butter crumble.
This provides a delicious contrast between the crunchiness of the peanut butter topping and the softness of the grape filling.
18. Grape Parfait
Fruity parfaits are ideal for the summer. They're refreshing and cool, allowing you to get a break from the heat. Plus, they're super straightforward to make, so you won't have to worry about getting hot and sweaty in the kitchen.
This glorious grape parfait recipe is simple yet flavorful. It contains layers of grapes, muesli, and yogurt. Thanks to a garnishing of mint, honey, and grapes, this parfait's flavor is boosted. Not to mention, this garnishing makes the dish look irresistible.
19. Chicken And Grape Wrap
A simple yet effective recipe, these wonderful wraps mix chicken with grape and ranch dressing. The addition of grapes provides a freshness to the dish, causing it to be bursting with fruity flavors.
This recipe is perfect for spring and summer picnics. Plus, it makes an effective lunchbox meal. So if you've grown bored of the same lunchtime meals, this chicken and grape wrap is sure to revolutionize things.
20. Grape Sorbet
Sorbet is an elegant dessert. This sophisticated grape sorbet recipe is sure to be a hit at dinner parties and other events.
Just ensure that you make it ahead of time because this recipe requires 5 hours of freezing time. This allows you to spend the rest of your day relaxing instead of cooking all day long.
Another reason to love this recipe is that you only need 4 ingredients, specifically frozen grapes, water, lemon juice, and sugar. Consequently, it's incredibly convenient and simple.
21. Fruit Kebabs
If you've been finding it hard to encourage your kids to eat fruit, this easy fruit kebab recipe is sure to do the trick. This is such a simple recipe, meaning that kids can easily get involved.
Though these fruit kebabs are straightforward, they have an eye-catching aesthetic that people are sure to adore. Containing differently colored fruits, these kebabs have a rainbow design.
Moreover, you can use whatever fruit you want, such as strawberries, kiwi, and grapes.
22. Roasted Cauliflower With Grapes And Almonds
Cauliflower can be a little boring on its own, so roasting it with grapes and almonds is an amazing way of revitalizing this vegetable. These additions ensure a broad spectrum of flavors and textures.
Aside from this, the cauliflower's flavor has been boosted by the addition of red pepper flakes, garlic, and a balsamic glaze. This recipe is a sensational side dish that will complement a wide variety of main dishes.
23. Italian Grape Cake
Our final pick is this sensational sweet Italian grape cake. This light and airy cake is loaded with phenomenal purple grapes. What we love most about this cake is the diverse range of flavors, which work together to bring out the flavors of the grapes.
These seasonings include cinnamon and orange zest, which give the cake earthy and citrus notes. This cake won't need much decoration. All you will need to do is lightly dust it with a little icing sugar for an appealing look.
Frequently Asked Questions
Are Grapes Healthy?
Yes, grapes are healthy. They count toward your 5-a-day consumption of fruit and vegetables. Just make sure that you have at least 1.5 or 2 cups of grapes, as this is considered a portion of fruit.
Grapes are sensational sources of potassium, vitamin C, and vitamin K. Thus, they are associated with numerous health benefits, such as reducing the risk of cardiovascular disease.
Can Grapes Be Frozen?
Yes! Freezing grapes is an incredible idea if you wish to extend their longevity. Once frozen, grapes last for approximately a year before they start to go off.
Closing Remarks
As demonstrated by the above recipes, grapes are surprisingly versatile. There are tons of different recipes for you to make with these juicy fruits. So what's stopping you? Why not make some gorgeous grape-flavored treats?!
- 15 Traditional Greek Breads - July 31, 2023
- 30 Delicious And Gluten-Free Cookie Recipes - July 29, 2023
- 30 Of The Best European Desserts - July 29, 2023


text:
Donald Trump, former President of the United States, applauded Friday's Supreme Court's decision to reverse Roe V. Wade and stated "it would work in everyone's favor. "
Trump stated, "This is following the constitution and giving back rights they should have given years ago. "
Trump answered a question regarding his message to pro-choice supporters. Trump replied, "I believe, in the end, it is something that works for everyone. "
Trump stated that "This brings all of it back to the States, where it belongs."
The ex-president was questioned if he felt that he played a part in Roe V. Wade's reverse. Trump-appointed three conservative Justices to the Supreme Court, Amy Coney Barrett, and Brett Kavanaugh. Trump stated, "God Made The Choice".
With those appointments, the high court became majority conservative, with Chief Justice John Roberts, Justice Samuel Alito, Justice Clarence Thomas, Gorsuch, Kavanaugh, and Barrett.
Justices Stephen Breyer, Sonia Sotomayor, and Elana Kagan, all appointed by Democrats, dissented from the majority opinion Friday to overturn Roe v. Wade.
The court's decision was in Dobbs v. Jackson Women's Health Organization. The court's decision was based on a Mississippi law that prohibited abortions after 15 weeks. A Republican-led state of Mississippi asked for the Supreme Court to reverse a lower court ruling that had repealed the ban.
"We end this opinion at its beginning, abortion raises deep moral questions." Justice Samuel Alito wrote in the court's opinion.
Alito's opinion began with a criticism of Roe V. Wade. The opinion held that the states had a legitimate right to protect "potential life", however, this interest was not strong enough to stop abortions before fetal viability. This occurs around 23 weeks into a woman's pregnancy.
Alito stated that the Court had not explained the reasoning behind this line, and even supporters of abortion have struggled to defend Roe's reasoning. "
Breyer, Kagan, and Sotomayor dissented, writing that Roe and Casey "understood the difficulty and divisiveness of the abortion issue," and by overturning the landmark Roe v. Wade ruling, a woman "has no rights to speak of."
They stated, "the Court had recognized that Americans have vastly different views about the morality and morality of ending a pregnancy. "
"So the Court struck a balance, as it often does when values and goals compete," they wrote. "It held that the State could prohibit abortions after fetal viability, so long as the ban contained exceptions to safeguard a woman's life or health."
"Today, the Court discards that balance," they wrote. "It says that from the very moment of fertilization, a woman has no rights to speak of."
"With sorrow–for this Court, but more, for the many millions of American women who have today lost a fundamental constitutional protection–we dissent," they wrote.


text:
Over the past couple of years I have noticed a brownish slippery substance on my 'tar and chips' yard and paths. this has increased 200% this year and is very obvious when the ground is wet. It seems to shrink when the ground dries. In time it turns to moss and looks terrible. I cant brush it off as this removes the chips. Is there any treatment you can suggest?
It can be associated with moss as both like damp ground conditions. In nature, this is found in bogland areas.
You can use any patio cleaner type product, such as Mosgo, or simply bleach, well diluted. Moss and algae will probably come back because the area is damp. Try to keep all leaves raked off the surface to reduce dampness.


text:
- Austria / Österreich
- Bosnia and Herzegovina / Босна и Херцеговина
- Bulgaria / България
- Croatia / Hrvatska
- Czech Republic & Slovakia / Česká republika & Slovensko
- Finland / Suomi
- France / France
- Germany / Deutschland
- Greece / ΕΛΛΑΔΑ
- Italy / Italia
- Netherlands / Nederland
- Nordic / Nordic
- Poland / Polska
- Portugal / Portugal
- Romania & Moldova / România & Moldova
- Slovenia / Slovenija
- Serbia & Montenegro / Србија и Црна Гора
- Spain / España
- Switzerland / Schweiz
- Turkey / Türkiye
- UK & Ireland / UK & Ireland
In the initial treatment, root filling materials are forced into the canal irregularities. These materials shield microbial infection that should be removed in order to effectively disinfect the canal. Standard NiTi files cannot remove these materials and bacteria particularly if there is a curvature in the canal.
Based on the shape-memory principles of the alloy MaxWire and thanks to his flexibility, the XP-endo Finisher R has the ability to expand and contract with an improved reach of 6 mm in diameter or 100-fold compared to a standard instrument of the same size. Its capacity to expand and its sickle shape allows it to access and clean areas previously impossible to reach.
The XP-endo Finisher R has a core diameter larger (ISO 30) than the XP-endo Finisher (ISO 25), making it slightly stiffer and also more efficient in removing root fillings materials adhering to the canal walls, especially in the curvature or oval areas.
Thanks to its small core size and its zero taper, the file benefits from incredible flexibility and show unparalleled resistance to cyclic fatigue. It can then contact and scrape the dentine surface and/or the root filling material without changing the original shape of the canal. This universal instrument should be used following any retreatment case of diameter ISO 30 or more.


text:
Solar Water Heating Component Businesses in Michigan
Renewable Energy Businesses in the World |
Renewable Energy Businesses in Michigan |
Solar Water Heating Component Businesses in the World |
Request Information or a Price Quote from All Businesses Listed Below |
BTF Solar Ltd - Geo-Renew Systems, Inc - GLE Solar Energy - Self Reliant Energy Company - Solar Research -
BTF Solar Ltd
affordable solar water heating with vacuum tube heat pipe technology
- Business type: retail sales, wholesale supplier
- Product types: solar water heating systems, solar water heating components, solar roofing systems.
- Address: POB 409, Fennville, Michigan USA 49450
- Telephone: 2692366179
- Web Site: http://www.btfsolar.com
- E-mail: Send Email to BTF Solar Ltd
Geo-Renew Systems, Inc
Renewable energy company that specializes in goethermal heating and cooling, solar photovotaics, solar water heating and small residential light commercial wind energy systems.
- Business type: retail sales
- Product types: geothermal energy systems, photovoltaic systems residential, solar water heating components, wind energy systems (small).
- Service types: installation, project development services, contractor services, maintenance and repair services
- Address: 3045 Grange Hall Rd. Suite 7, Holly, Michigan USA 48442
- Telephone: 248-531-0325
- Web Site: http://www.georenew.com
- E-mail: Send Email to Geo-Renew Systems, Inc
GLE Solar Energy
GLE Solar Energy is a Michigan-based company that provides a revolutionary solar water heating product. We have designed a unique collector specifically for the North American market: the only solar technology that makes best use of solar radiation when it is mounted vertically.
- Business type: manufacturer
- Product types: solar pool heating system components, solar water heating components collectors evacuated tube, solar thermal energy, solar collectors evacuated tube.
- Address: 1776 Hilltop Road, Saint Joseph, Michigan USA 49085
- Telephone: 269-408-8276
- Web Site: http://www.glesolar.com
- E-mail: Send Email to GLE Solar Energy
Self Reliant Energy Company
Small Company focused on Small Business Owners, Farms and Michigan Residents. Self Reliant Energy Continously researches and promotes the use of renewable energy and incentives / tax credits for small business and residential independence. BPI Certified. NABCEF
- Business type: Sales, Service, Installation of Residential Renewable Energy Equipment.
- Product types: Building Performance Institute Home and Buisness Weatherization, Solar Air Heating Systems (passive), Solar Thermal Heating Systems, Photovoltaic Systems (PV), Wind Energy Systems, Biomass Energy Systems & Components, Hybrid Power Systems, Grey Water Collection and Waste Heat Recovery, Anaerobic Digestion Systems, Geothermal Systems. .
- Service types: Consulting, Design, Installation, Engineering, Education and Training Services, Research Services, Maintenance and Repair Services.
- Address: 10192 Sargent Road, Fowlerville, Michigan USA 48836
- Telephone: 517-348-7115 / 517 256 2322 Cell
- Web Site: www.selfreliantenergycompany.com
- E-mail: Send Email to Self Reliant Energy Company
Solar Research
- Business type: manufacturer, retail sales
- Product types: solar water heating systems, solar water heating components, hydronic water heating systems.
- Address: P.O. Box 869, Brighton, Michigan USA 48116
- Telephone: (810) 227-1151
Not Listed? Add your business listing to the Source Guides business directory
Can't Find It? Search the entire Source Guides site using keywords:
Renewable Energy Businesses |
Business Search |
Advertise |
Renewable Energy News |


text:
Harry Styles stepped out with his girlfriend Taylor Russell for the first time since his stalker was charged for causing him harm and distress.
The singer was seen strolling the streets of London with the Lost in Space actress. The couple twinned in black outfits for their outing. Harry sported a black jacket with matching shorts. He kept it low-key with a dark blue baseball cap and black sunglasses.
Taylor, on the other hand, donned baggy jeans with a long black coat and a black hat. She completed her look with gold hoop earrings and black bag.
The couple were previously seen early this month on a vacation with James Corden in Anguilla.
During his trip, the As It Was singer was stalked by Myra Carvalho. Per an insider, "Harry was really shaken up. He had spent the start of the month in Anguilla with Taylor and James Corden and he'd had a great break."
"This happened not long after he had come back. Harry just wants to carry on as normal, but this has been concerning."
Carvalho was charged on Tuesday for "harassment – which amounted to stalking causing serious alarm or distress," per The Sun.


text: THE AREA'S PREMIER SENIOR LIVING COMMUNITY PURPOSEFUL LIVING Whether you're looking for Independent Living, Assisted Living or Inspiritás - Memory Care, Woodland Hills is committed to taking senior living to the next level. From our diverse enrichment and social programs including RUI University to our exceptional fine dining experience, we deliver valued living all in the comfort of your new picturesque home. At Woodland Hills, our residents enjoy: · Studio, one and two bedroom units with granite counter tops and stainless steel appliances · Delicious, nutritious meals prepared by our Executive Chef, served in a restaurant-like setting · 24-7 On-site Licensed Nurse · A full spectrum of life enrichment programing and events · RUI University program including variety of instructional and professional courses · Full service spa with massage therapy, pedicure/manicure service, and hair stylists · Library, theatre, gym, and bistro · Convenience of physical, occupational and speech therapy on-site · Housekeeping and laundry services · Leash on Life - Pet friendly services Call 540-682-7500 for more information, and to schedule a tour. 3365 OGDEN RD · ROANOKE, VA 24018 · 540-682-7500 · WWW.RUI.NET/WOODLAND-HILLS · @WOODLANDHILLSSENIORLIVING A RETIREMENT UNLIMITED, INC. COMMUNITY · FAMILY-OWNED & OPERATED IN VIRGINIA FOR MORE THAN 35 YEARShttp://WWW.RUI.NET/WOODLAND-HILLS http://WWW.RUI.NET/WOODLAND-HILLS


text:
Search & Browse
Searching or browsing for products on fishersci.ca is easy. All you have to do is enter keywords, catalog numbers, CAS numbers, or manufacturer part numbers in the main search box located in the blue bar at the top of the site. Or browse top products and categories using the Shop Products menu.
Search and browse results can be filtered by brand or product specifications. Simply select refinements to the left of the results.
Use our custom search tools to find products for your specific needs from a wide range of suppliers.
Get key product documentation using our Safety Data Sheet and Product Certificate searches.


text:
A UX SaaS vendor is seeking a Strategic Account Manager (m/f/d), for the DACH region.
This role will allow you to reach your potential and manage Key Accounts, in the Mid-Market and Large Enterprise segment, focusing on up-selling and reselling, and collaborating with a highly experienced EMEA sales team.
* Build and develop relationships with targeted clients
* Develop a sales strategy and plan within the region
* Achieve/overachieve your quotas quarter by quarter
Skills and experience required:
* +5 years of quota-carrying experience in Account Management, ideally in the SaaS space
* self-motivated, goal-oriented, and able to work independently
* fluency in German and English language
* understanding and using a preferred Sales Methodology (MEDDPICC, MEDDIC, SPIN, etc.)
What this job/company offers you:
* Clear career progression path
* Home office flexibility
* Competitive salary with numerous additional benefits
How to apply:
If you are interested in this opportunity or in a career change, please apply, or reach out directly to Evelyn Cseh, at +49 30 726211411, for a confidential discussion.


text:
BERNIE: I'm not paranoid, but those nasty tweets from my supporters are probably actually by Russian agents.
Meanwhile, everyone on the Dem debate stage seems unbalanced — they look so angry that they're about to either hit someone or burst into tears. It's bizarre.
UPDATE: Ouch.
Shocking denouement — after losing the race, Bloomberg decides Dems are too crazy to rule, endorses Trump for general election. . . .


text:
Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium
Preprint posted on 15 April 2020 https://www.biorxiv.org/content/10.1101/2020.02.04.933440v2
Article now published in eLife at http://dx.doi.org/10.7554/eLife.57907
Hooked to cilia: a novel nanobody-based tool opens the way for acute manipulation of primary cilia.
Selected by Gustavo AguilarCategories: cell biology, synthetic biology
Cells have developed an extraordinary repertoire of compartments, from cellular sub-domains to organelles, vesicles and membraneless organelles. This way, most cellular functions are restricted in space, ensuring the presence of all the required molecular players and minimising the crosstalk with other cellular processes, among other benefits. When trying to manipulate our favourite cellular activity, either to decipher its function or to exploit its possible benefits, it is critical to consider this spatial component, otherwise we could easily be mistaken in our interpretation.
A paradigmatic case of signalling compartmentalisation is the primary cilium, sometimes referred to as the "cell's antenna". This surface projection harbours distinct receptors and downstream signalling components in a Lilliputian reaction volume, permitting highly sensitive and fast signal transduction. Among all the cell's second messengers, cAMP plays a central role in signal transduction in the cilium, acting downstream of diverse GPCRs. In recent years, the direct observation and manipulation of cAMP has been of major interest to understand the functioning of cilia. Currently, two light-activated enzymes permit both the production and hydrolysis of cAMP: the photo-activated adenylate cyclase (bPAC)1 and the light-activated phosphodiesterase (LAPD)2. Despite the availability of optogenetic tools, restricted manipulation of cAMP to the primary cilia has been impossible, hampering the dissection of its local functions.
The tool
Hansen, Kaiser et al.3 propose a novel way to localize virtually any GFP or mCherry tagged protein to the primary cilium. They envisioned that if tagging of the protein of interest per se did not affect protein function, they could use such moiety to "drag" the protein to the cilium. To do so they made use of nanobodies, which are single-chain protein binders derived from heavy-chain only antibodies. Nanobodies have comparable binding affinity to normal antibodies, which together with its small size and solubility, makes them perfect for intracellular usage4. In their case, a nanobody recognizing GFP or mCherry was fused to a cilium-specific protein scaffold, resulting in the localization of the nanobody in the cilium. Upon co-expression of this nanobody and a GFP- or mCherry-tagged protein the latter will be re-localized to the cilium.
The authors apply this method to localize bPAC and LAPD in the cilium and this way achieve local modulation of cAMP levels.
Scheme of the new cilia-targeting approach. Extracted from Figure 2 of Hansen, Kaiser et al. 2020.
The specifics
The authors show that direct fusion of the bPAC and LAPD to protein domains targeted to the primary cilium (in particular the N terminal region of the mouse Nphp3) resulted in ciliary localization but reduced or impeded enzymatic activity.
To overcome this limitation, they developed a bipartite solution to target proteins of interest to the cilium. On one hand, they fused the cilia-specific "scaffold" mentioned before (truncated mNphp3) to an anti-mCherry nanobody, a highly specific protein binder that recognizes the protein mCherry with nanomolar affinity. This cilia-localised nanobody was co-expressed with the mCherry-tagged Optoenzymes bPAC or LAPD, resulting in the latter being "dragged" to the cilia by the nanobody. They confirmed that the nanobody-mediated relocalization of bPAC and LAPD resulted in fully functional enzymatic activity.
Relocalization of LAPD-mCherry to the cilia. Nanobody in green. LAPD-mCherry in red. Primary cilium in cyan. Dapi in blue. Modified from figure 2 of Hansen, Kaiser et al. 2020.
Next a FRET based reporter of cAMP was targeted to the primary cilia in a similar way. In this case, the authors used an anti-GFP nanobody instead of the anti-mCherry. mNphp3-anti-GFP nanobody fusion could bind the fluorescent proteins of the FRET reporter and localise it to the cilium. The reporter could still detect changes in cAMP concentration.
The new toolkit was then used to investigate the effect of local levels of cAMP in regulating cilia length. Their data supports that local cAMP in the cilium increase its length whereas global cAMP increase is associated with cilia shortening.
Finally, the authors showed that the nanobody-mediated cilia targeting can be used in vivo. Expression of the cilia localised anti-mCherry nanobody in zebrafish could enrich in the cilia an otherwise cytoplasmic RFP.
Why I think this paper is important.
The specific subcellular targeting of functional domains is a challenge with which the authors deal elegantly. Targeting functional domains for long periods may result in unpredictable outputs. In this study, the use of optogenetic tools overcomes this problem, adding temporal control to the setup. In this and few other recent papers5,6,7 the combination of nanobodies and optogenetic tools is starting to flourish and will garner exciting methods for cell biology.
The use of nanobodies against commonly used fluorescent tags allows relocalization of many already existing GFP- and mCherry-tagged proteins to the cilia and will inspire other scientist to tackle the function of the cilia in a much more reliable and imaginative way.
Questions to the authors.
Recently, the list of intracellular protein binders has increased dramatically, Alphatag, Moontag and Frankenbodies are only the tip of the iceberg. These binders recognise small non-fluorescent tags. Do the authors contemplate the use of such binders to be able combine their different toolkit components at the same time? What possibilities would this open?
The validation of the cilia-targeted FRET sensor was done by stimulating the production or hydrolysis of cAMP by drugs. What do the authors think is the sensitivity of the FRET sensor? Would it be able to detect physiological changes in cAMP?
The authors also showed that localisation of bPAC to the cilia at high levels could by itself (without photo-activation) result in a change in cilia length. Could the authors elaborate on this? Does this mean that bPAC has a very low basal activity? Or could it be due to the expression of the mNphp3 scaffold?
The tools seem ready to start exploring when is cAMP produced in the cilia and dissect its role. Apart from control of cilia length, which are the biological questions where the authors think these tools will be useful in the next years?
- Stierl M, Stumpf P, Udwari D, Gueta R, Hagedorn R, Losi A, Gartner W, Petereit L, Efetova M, Schwarzel M, Oertner TG, Nagel G, Hegemann P (2011) Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. The Journal of biological chemistry 286: 1181-8
- Stabel R, Stüven B, Hansen JN, Körschen HG, Wachten D, Möglich A (2019) Revisiting and Redesigning Light-Activated Cyclic-Mononucleotide Phosphodiesterases. J Mol Biol 431: 3029-3045
- Hansen, J. N., Kaiser, F., Klausen, C., Stueven, B., Chong, R., Boenigk, W., … & Wachten, D. (2020). Nanobody-directed targeting of optogenetic tools reveals differential regulation of cilia length.BioRxiv.doi: https://doi.org/10.1101/2020.02.04.933440
- Helma, J.; Cardoso, M.C.; Muyldermans, S.; Leonhardt, H. (2015) Nanobodies and recombinant binders in cell biology. Cell. Biol.209, 633–644.
- Deng, W., Bates, J. A., Wei, H., Bartoschek, M. D., Conradt, B., & Leonhardt, H. (2020). Tunable light and drug induced depletion of target proteins. Nature Communications, 11(1), 1-13.
- Yu, D., Lee, H., Hong, J., Jung, H., Jo, Y., Oh, B. H., … & Do Heo, W. (2019). Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Nature methods, 16(11), 1095-1100.
- Gil, A. A., Zhao, E. M., Wilson, M. Z., Goglia, A. G., Carrasco-Lopez, C., Avalos, J. L., & Toettcher, J. E. (2019). Optogenetic control of protein binding using light-switchable nanobodies. BioRxiv, 739201. doi: https://doi.org/10.1101/739201
Posted on: 5 May 2020
doi: https://doi.org/10.1242/prelights.20026
Read preprintHave your say
Sign up to customise the site to your preferences and to receive alerts
Register hereAlso in the cell biology category:
Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2
Excitable Rho dynamics drive cell contractions by sequentially inducing ERM protein-mediated actin-membrane attachment and actomyosin contractility
Nanos2+ cells give rise to germline and somatic lineages in the sea anemone Nematostella vectensis
Also in the synthetic biology category:
Discovery and Validation of Context-Dependent Synthetic Mammalian Promoters
Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM
Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast
preListscell biology category:
in the'In preprints' from Development 2022-2023
A list of the preprints featured in Development's 'In preprints' articles between 2022-2023
List by | Alex Eve, Katherine Brown |
preLights peer support – preprints of interest
This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.
List by | preLights peer support |
The Society for Developmental Biology 82nd Annual Meeting
This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.
List by | Joyce Yu, Katherine Brown |
CSHL 87th Symposium: Stem Cells
Preprints mentioned by speakers at the #CSHLsymp23
List by | Alex Eve |
Journal of Cell Science meeting 'Imaging Cell Dynamics'
This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.
List by | Helen Zenner |
9th International Symposium on the Biology of Vertebrate Sex Determination
This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.
List by | Martin Estermann |
Alumni picks – preLights 5th Birthday
This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.
List by | Sergio Menchero et al. |
CellBio 2022 – An ASCB/EMBO Meeting
This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.
List by | Nadja Hümpfer et al. |
The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!
List by | Osvaldo Contreras |
EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)
A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.
List by | Alex Eve |
A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020
List by | Ana Dorrego-Rivas |
Planar Cell Polarity – PCP
This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.
List by | Ana Dorrego-Rivas |
BioMalPar XVI: Biology and Pathology of the Malaria Parasite
[under construction] Preprints presented at the (fully virtual) EMBL BioMalPar XVI, 17-18 May 2020 #emblmalaria
List by | Dey Lab, Samantha Seah |
Cell Polarity
Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.
List by | Yamini Ravichandran |
Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20
List by | Maiko Kitaoka et al. |
3D Gastruloids
A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.
List by | Paul Gerald L. Sanchez and Stefano Vianello |
ECFG15 – Fungal biology
Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome
List by | Hiral Shah |
ASCB EMBO Annual Meeting 2019
A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)
List by | Madhuja Samaddar et al. |
EMBL Seeing is Believing – Imaging the Molecular Processes of Life
Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019
List by | Dey Lab |
Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.
List by | Sandra Malmgren Hill |
Lung Disease and Regeneration
This preprint list compiles highlights from the field of lung biology.
List by | Rob Hynds |
Cellular metabolism
A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.
List by | Pablo Ranea Robles |
BSCB/BSDB Annual Meeting 2019
Preprints presented at the BSCB/BSDB Annual Meeting 2019
List by | Dey Lab |
This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.
List by | Sandra Franco Iborra |
Biophysical Society Annual Meeting 2019
Few of the preprints that were discussed in the recent BPS annual meeting at Baltimore, USA
List by | Joseph Jose Thottacherry |
ASCB/EMBO Annual Meeting 2018
This list relates to preprints that were discussed at the recent ASCB conference.
List by | Dey Lab, Amanda Haage |
Also in the synthetic biology category:
'In preprints' from Development 2022-2023
A list of the preprints featured in Development's 'In preprints' articles between 2022-2023
List by | Alex Eve, Katherine Brown |
EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)
A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.
List by | Alex Eve |
EMBL Conference: From functional genomics to systems biology
Preprints presented at the virtual EMBL conference "from functional genomics and systems biology", 16-19 November 2020
List by | Jesus Victorino |
Antimicrobials: Discovery, clinical use, and development of resistance
Preprints that describe the discovery of new antimicrobials and any improvements made regarding their clinical use. Includes preprints that detail the factors affecting antimicrobial selection and the development of antimicrobial resistance.
List by | Zhang-He Goh |
Advances in Drug Delivery
Advances in formulation technology or targeted delivery methods that describe or develop the distribution of small molecules or large macromolecules to specific parts of the body.
List by | Zhang-He Goh |