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Locations: Ridgefield, Danbury
Educational Background:
Undergraduate studies: Brandeis University
Medical school: Mount Sinai School of Medicine
Residency: The Mount Sinai Hospital
Certificates, Professorships, and Affiliations:
- Fellow: American Academy of Otolaryngology – Head and Neck Surgery
- American Rhinologic Society
- New York Facial Plastic Surgery Society
- Alpha Omega Alpha Medical Honor Society
Dr. Klarsfeld is a practicing ENT physician specializing in Head & Neck surgery, Thyroid & Parathyroid surgery, and Closed Rhinoplasty. He is a resident of Ridgefield and sees patients at our Danbury & Ridgefield offices.
Dr. Klarsfeld has received numerous Top Doctor awards including:
- Top Doctors: New York Metro Area (book series): 6th, 7th, 8th, 9th. 10th, 11th, 12th, 13th, 14th, 15th, 16th, 17th, 18th and 19th Edition
- Connecticut Magazine Connecticut Top Doctors | Moffly Media Magazines Fairfield County Top Doctors: 2016, 2017, 2018, 2019, 2020, 2021, 2022, 2023, 2024
- Top Doctors: New York Metro Area (digital guide): 2016, 2017, 2018, 2019, 2020, 2021
- America's Most Honored Doctors | The American Registry: 2021, 2022
"Friendly Staff, Comfortable atmosphere, The group worked tirelessly with my Insurance co. to get everything approved in a very shot time frame. Dr. Klarsfeld
takes the time to listen and is thorough in his examination and execution of the care provided. 100% recommended."
"Dr. Klarsfeld
is an amazing doctor. Highly recommend him. He saved my life!! I needed an MRI and was denied twice. He actually pushed for me and final received an MRI which they found a tumor the size of an Apple where my brain is. If it wasn't for his knowledge and care for all patients I would not be writing this review. He has visited me 3 times at the hospital and made me feel special. He has the biggest heart!!! So Grateful..."
"Dr. Klarsfeld is not only a skilled physician and surgeon; he is personable and has a pleasant bedside manner. How refreshing! Since my Septoplasty, I can sleep on my side all curled up. I no longer get waves of nausea and sinus headaches each afternoon. The office staff is pleasant and service oriented, especially Flo in surgical scheduling, messages don't get lost. They are reliable and follow up the way they say they will."
~ P.S. - Wilton, CT
"I was so glad that I went through with the Balloon Sinuplasty Surgery! Dr. Klarsfeld, did an excellent job!
And then to find out that the Recovery was minimal, was icing on the cake! I'm finally draining..." ~ D.J.
"Made an Appointment to evaluate my Tonsils. On one side of my throat i had a normal one then the other had a very enlarged one I have had most of my life. Dr. Klarsfeld was incredibly helpful and understanding, he told me it would be beneficial to have them out now rather than even later in life. Unfortunately the experience adults go through with tonsil removal is pretty horrible but the office and himself checked up on me after the surgery and now being a month after all is well. I highly recommend Dr. Klarsfeld he did an amazing job. :)" ~ L.B.
"This was my first time ever having a health concern so obviously I was a little nervous. Dr. Klarsfeld made me incredibly comfortable and his attention to detail and experience calmed my nerves. He came highly recommended and he certainly didn't disappoint. The procedure went smoothly and I'm well on my way to being fully mended. I couldn't have asked for a better doctor. Thank you and your team Dr. Klarsfeld!" ~ L.C.
"Dr Klarsfeld is awesome. He is thorough, compassionate, informative, and reassuring. Performed thyroidectomy. Process was seamless, even during covid." ~ R.S.
"Dr. Klarsfeld was my immediate choice when I learned that I needed thyroid surgery. He had already repaired my deviated septum and even diagnosed my adult tonsillitis. He is a compassionate physician with a genuine sense of concern for his patients." ~ A.K.
"My 21 year old son went to Dr. Klarsfeld during the pandemic for constant throat issues like strep. Once we found out that he needed a tonsillectomy, we were able to schedule the surgery as soon as everything opened again. Tina in Dr. Kalrsfeld's office kept us informed and updated all along about procedures due to Covid. Dr. Klarsfled prepared my son (and me) for what to expect for a tonsillectomy at this age. His recovery was exactly as he said it would be. He had a post-op appointment after one week and that was helpful to get the reassurance we needed that his recovery was on track. Within 13 days of surgery, he was about 90 percent. Again, having another appointment on the 13th day of recovery before sending him back to college was very helpful. I can't imagine having a better experience for something this painful and we are extremely grateful to Dr. Klarsfeld." ~ A.D.
"Dr. Klarsfeld as been nothing sort of amazing. I was diagnosed with Thyroid Cancer and he kept me calm and comfortable throughout my process. He completed both surgeries I required and has been extremely supportive as I go through this life change. He actually cares which is important. Please see him if you are ever in my position. I would not want to see anyone else!" ~ B.B.


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All Photos
Jamie Karaffa Wins ME Milken Award
Bruce M. Whittier Middle School | Poland, ME | Apr 12, 2022The Milken Educator Award includes a cash prize—but how much? One digit at a time, students hold up cards representing the amount. When Maine Department of Education Deputy Commissioner Dan Chuhta holds up a third zero, the assembly starts to buzz. Hey, $25,000 is a lot of money!
In this series:
Jamie Karaffa (ME '21)
In this photo:
Stephanie E. Bishop (VA '01)
All photos should be credited to "Milken Family Foundation" unless otherwise noted.
Download a high-resolution version of this image

subset: commoncrawl


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Advertisement |
If he knew he had been targeted as the offender, we were convinced that we would never catch him
Mexico arrests suspect in '07 Ohio deaths Apr 22, 2009
This guy's just a pig. I can't explain why someone would do something like this
Prosecutor: Morgue worker raped corpses Feb 26, 2009
Those babies deserve a home. I would hope their gifts don't go to waste
Mom of two sets of triplets indicted Feb 06, 2008
Once a case goes in there, we don't hear from him for eight years
Death penalty cases taken away from judge Jan 25, 2008
I am very grateful for the person who brought this to our attention
Ohio woman charged in 'pot tot' case Sep 16, 2010
Joseph T. "Joe" Deters is an American politician of the Republican party who currently serves as Prosecuting Attorney for Hamilton County, Ohio. He is a 1975 graduate of St. Xavier High School in Cincinnati.
Deters began his career in public service in 1982 as an Assistant Hamilton County Prosecutor. He was elected to the position of Hamilton County Clerk of Courts in 1988. In 1992, Joe returned to the Hamilton County Prosecutor's office when he was elected Prosecutor. He was subsequently reelected in 1996.
In 1999, Deters was sworn in as Ohio's 44th Treasurer of State. As Treasurer, he was responsible for collecting, managing and investing over $11 billion in assets for the State of Ohio. In the fall of 2004, Deters became a write-in candidate for his former position, Hamilton County Prosecuting Attorney. He was elected Hamilton County Prosecuting Attorney on November 2, 2004 with nearly 60% of the vote. On April 1, 2005, Deters and Matthias Heck filed a federal lawsuit, Citizens for Tax Reform v. Deters.


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¡Que viva | Animation film | Hand paintend film and photocopy | 2019
Filmmaker Festival Milan, 2019 |
Short Movie Club Film Festival, Unfiltered Cinema, Minsk, Belarus, 2020 |
66 International Short Film Festival Oberhausen, Open Screening online, Germany, 2020 |
Lonely at the party, Metaforte, Cavallino-Treporti Venezia, 2020 | ISFFC International Short Film Festival Canton China, 2020
| T-short animated Film Festival, official selection in Experimental Film out-of-competition, 2021 |
VAA, Video Art Award, Centro Luigi Di Sarro, winner, 2022 | Indocili, Cinema Beltrade, Milano, 2023 | Best Artist Film at The Next Generation Film Festival, 2022 |
¡Que viva is a short animation on the theme of bullfighting, in which looks and faces are like shotguns or a bullfighter.
A reflection on the struggle and the act of seeing, the encounter between the victim and the viewer's gaze through bullfighting, seen both
as a ritual sacrifice and as a cruel struggle.
The images mix hand paintings on glass and analogically modified frames of Sergej Ejzenstejn's Que viva Mexico!
© 2019 · all right reserved / © all content on this website is the work of Giulia Savorani and can not be used or distributed without written consent by the artist.


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Wednesday was in talks ever since 2013 but it was in February 2021 that production started. Supernatural horror as a genre has emerged totally in a different way with the mixture of comedy.
And that's what the current audience is like and due to the same reason makers are turning their choices and making things go according to the audience.
Talking about any current supernatural comedy that is right now in talk according to the release date then some have a different mixture of some other genres like coming of age. To get everything in one plate we have Wednesday of Netflix that is right now and talk related to its release.
Wednesday is an upcoming American television series that have comedy horror as well as supernatural element with coming of age. It is based upon the character called Wednesday Addams and created by Alfred with Miles.
Wednesday Release Date
Talking about the release date then Wednesday is going to come on Netflix on 23 November 2022. It will have 8 episodes and every episode is going to release in one go. Some multiple directors and writers have contributed to the making of the series.
Coming to development then Wednesday was in talks ever since 2013 but it was in February 2021 that production started. Soon after the production ended, makers decided to announce the release date and trailer.
Also Read: Heartbreak High Season 2 Renewal Status, and Other Details
The Plot
The story of Wednesday is related to the character named Wednesday Addams who is a high school student. She tries to master her psychic powers and stop a mysterious killing that is happening in her town.
So to solve this mystery that affected her family almost 25 years ago, she started an investigation related to her new relationship.
Also Read: The Crown Season 6 Renewal Status, and Other Details
The Cast
The cast and characters of Wednesday are totally up to the point as every actor has given their best to their characters. Whether we talk about the leading or the supporting one, everyone has contributed their best to it.
So here are the names of Wednesday's cast and characters:
Jenna Ortega would be as Wednesday Addams
Gwendoline Christie would be as Larissa Weems
Riki Lindhome must be as Dr. Valerie Kinbott
Jamie McShane would be as Sheriff Donovan Galpin
Hunter Doohan in the role of Tyler Galpin
Percy Hynes White would be Xavier Thorpe
Emma Myers as Enid Sinclair
Joy Sunday must be as Bianca Barclay, a siren
Georgie Farmer in the role of Ajax Petropolus
Naomi J. Ogawa as Yoko Tanaka, a vampire
Christina Ricci would be Marylin Thornhill
The excitement of fans is totally up to the level it needed to be and that's the reason why makers are going to give their best through 8 episodes that are going to release on Netflix.


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If you're the praying type, please keep the Britt family in mind. Vera Britt, the mother of the many Britt brothers to play for the Tide, underwent surgery on Monday after an accident over the weekend. Justin Britt is understandably absent from campus and will likely miss this weekend's game, and Antoine Caldwell is expected to move over to LG in his place.
The Columbus Ledger-Enquirer reports that the coaching staff plans to drill the idea of sustaining intensity the entire game this week:
Also from the article, just one more quote that proves why Saban is the right guy for this job:
And on that note, from his Monday press conference:
Congratulations to Wallace Gilberry, the SEC Defensive Lineman of the Week:
Defensive End
6-3 * 267 * Senior
Bay Minette, Ala. (Baldwin County HS)
Gilberry led the Crimson Tide defense with 11 total tackles (nine solos), 4.5 tackles for loss, including two sacks, and two quarterback pressures, in Alabama's 30-24 win against Houston. His sack of Houston's quarterback in the first quarter led to a safety and put the Tide ahead, 9-0. Gilberry is tied for third on the team with 37 total tackles, first among defensive linemen. He is second in the SEC with nine tackles for loss this season.
The Mobile Press-Register notes that Ed Orgeron wanted Terry Grant to come to Ole Miss:
In the end, Orgeron couldn't even get Grant to The Grove.
"He's a loud guy if you've ever met him," Grant said of Orgeron. "I had a feel of where I wanted to be. I just kind of felt that wasn't the place for me."
It also didn't help that the Orgeron's recruiting pitch involved the hurling of boulders. |
I read about the amazing finish to the Western Michigan/Akron game at SMQ yesterday:
Akron's Alphonso Owen took the free kick from Broncos punter Jim Laney at the 11-yard line and began upfield. In the grasp of WMU's Boston McCornell at about the 25-yard line, Owen found teammate Andre Jones streaking up the left sideline.
Jones grabbed the short lateral and reversed field, beating WMU's coverage deep into Broncos territory before slipping past C.J. Wilson at the 5-yard line and ducking into the end zone for the victory after time had expired.
Naturally, Orson has the video:
Don't lie and say you didn't light up like a kid at Christmas as soon as the announcer starts screaming "Andre Jones is in the open field! Andre Jones with one man to beat! Andre Jones is going to go to the five to the two to the one TOUCHDOWN! THE ZIPS WIN IT'S OVER IT'S OVER THE ZIPS HAVE WON THE GAME!" because, even though I have no vested interest in either team and wouldn't have even known the outcome if it weren't for this incredible finish, damn it, people, I was grinning ear to ear watching that. God, I love football...
The big meme roaring around the CFB blogs yesterday was the news that Joe Paterno was allegedly involved in a road rage incident. Via Black Shoe Diaries:
Meanwhile, Block U has particularly amusing take on it as well.
Finally, your morning YouTube comes courtesy of Marty Robbins:


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Your basket is currently empty!
2.5kg Outdoor Ascot Feeder with Rain Guard
£11.50 excl VAT
In stock
This 2.5 kilo capacity feeder is a smaller version of our popular Blenheim outdoor feeder. A secure rain guard is supplied to keep out the rain. A quality product designed & made in the UK. it can be used with or without feet which are supplied to suit the size of birds. an anti-scratch ring is built in as standard to minimize wastage. This new feeder is designed for the small scale poultry keeper and will fit comfortably inside our increasingly popular all-in-one arks & housing.
Additional information
Weight | 1 kg |
HS_CODE | 84362900 Origin – United Kingdom |


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Date: March 4th Time: 03:00 pm For: Family members of a person who has died an expected death in the past 36 months, at home, in hospital...
Volunteer Information Session – Enderby
Friday, February 23rd – 6:00 – 7:30 PM
Hello Enderby!
Are you interested in volunteering with the North Okanagan Hospice Society (NOHS)?
NOHS is committed to providing a rewarding experience for our volunteers and an exceptional level of service to our residents, clients and our community.
Hospice volunteering is suitable for dedicated and caring people who are interested in supporting palliative individuals and being present throughout their journey.
Although it can be difficult for some, for many it is the most rewarding and fulfilling way to help other people.
We offer both direct and indirect service roles, so depending on your level of comfort, you can provide help and support in a variety of ways.
NOHS is currently looking for volunteers who are interested in:
– Being a Nav-CARE volunteer who visits people with a serious illness in their home
– Assisting with daily food services – Providing office and reception assistance – Helping with fundraising activities and special events – Nurturing our beautiful gardensPlease note that at this time, due to COVID-19 regulations set by the Interior Health Authority, all NOHS volunteers must be fully vaccinated in order to volunteer at hospice as well as provide results from a standard tuberculosis (TB) test.
To register for this Volunteer Information Session, please contact Jenna, Community Program Manager at [email protected] or 250-503-1800 ext. 115.
Pre-registration is required for this event.


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An Econometric Analysis of Price Behaviour in Pakistan
In this paper an attempt has been made to explore the major causes of price level changes in West Pakistan during the past thirteen years and to determine their relative importance in explaining the price fluctuations. A supplementary object of the paper is to develop a predictive mechanism which may be used to forecast the response of price level to changes in the explanatory variables used in the regression model. There is vast literature on inflation theory [3] but not so much on quantitative evidence. Broadly, there are three groups of theories of inflation: the demand pull theories, which state that inflationary pressures result from aggregate demand exceeding aggregate supply at full employment; the cost-push theories, which stress the producers' power to pass on cost increases in higher prices even when demand remains unchanged. The third group of theories, which take a mid-way position between the demand-pull and the cost-push theories, are a number of structural theories, notably those associated with the names of Ackley, Eckstein, and Schultze [1,5,11]. According to Ackley, inflation results from mark-up of prices. He considers the price policies of the firms and the wage policies of the labour unions to be responsible for inflation. He puts forward the hypothesis that mark ups used by business in setting prices and those applied by the labour unions to their cost of living for getting higher wages tend to rise in an inflationary situation which results in pyramiding of costs. Professor Otto Eckstein advances the hypothesis that inflation may be caused by price increases in certain bottleneck industries even when there is no over-all excess demand in the economy.


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About "Keep On Loving You"
"Keep On Loving You" is a rock ballad written by Kevin Cronin and performed by American rock band REO Speedwagon. It features the lead guitar work of Gary Richrath. The song first appeared on REO Speedwagon's 1980 album Hi Infidelity. It was the first REO Speedwagon single to break the top 50 on the U.S. Billboard Hot 100, reaching the number-one spot for one week in March 1981. The single was certified platinum for U.S. sales of over one million copies. It peaked at number seven on the UK Singles Chart. "Keep On Loving You" has been a mainstay on 1980s soft rock compilations and has appeared on dozens of 'various artists' compilation albums, as well as several REO Speedwagon greatest hits albums.Ultimate Classic Rock critic Matt Wardlaw rated it REO Speedwagon's all-time greatest song.
Top songs by REO Speedwagon
- Keep On Loving You
- Can't Fight This Feeling
- Take It On The Run
- Just For You
- Don't Let Him Go
- Love Is A Rock
- Keep Pushin'
- Lightning
- Like You Do
- Back On The Road Again
- Find My Fortune
- Dream Weaver
- In My Dreams
- Music Man
- Only The Strong Survive
- Can't Get You Out Of My Heart
- I Wish You Were There
- 157 Riverside Avenue
- Take Me
- Time For Me To Fly
- Lost In A Dream
- Give Me A Ride (roller Coaster)
- Love In The Future
- Blazin' Your Own Trail Again
- Lucky For You
- Movin'
- Being Kind (can Hurt Someone Sometimes)
- Do Your Best
- Break His Spell
- Any Kind Of Love
- Drop It (an Old Disguise)
- Golden Country
"Keep On Loving You" video by REO Speedwagon is property and copyright of its owners and it's embedded from Youtube.
Information about the song "Keep On Loving You" is automatically taken from Wikipedia. It may happen that this information does not match with "Keep On Loving You".
SONGSTUBE is against piracy and promotes safe and legal music downloading. Music on this site is for the sole use of educational reference and is the property of respective authors, artists and labels. If you like REO Speedwagon songs on this site, please buy them on Itunes, Amazon and other online stores. All other uses are in violation of international copyright laws. This use for educational reference, falls under the "fair use" sections of U.S. copyright law.


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There are only so many times you can break the status quo before becoming the new normal. Queens of the Stone Age tries to maintain its defiance as anti-heroes on their seventh studio album, "Villians." It's debatable if their sound changed anything but for the band, the direction did open up new electronic and funk elements to create a uniquely disco-tinged hard rock record.
Out of the ashes of desert stoner band Kyuss, Josh Homme created Queens of the Stone Ages. Despite being an insufferable scumbag, Hommes was able to carve a niche into the hard rock world of the time dominated by nu-metal and Nickelback clones. The band then would make a uniquely experimental discography that never broke convection but consistently snarkily defied it. They balanced the sexy and accessible sides of rock with the weird and wild, making them one of the biggest rock bands of the 21st century.


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Back to Journals » International Journal of Nanomedicine » Volume 12
Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model
Authors Zheng Y, Qin JB, Wang X, Peng ZY, Hou PY, Lu XW
Received 28 July 2016
Accepted for publication 4 October 2016
Published 21 December 2016 Volume 2017:12 Pages 61—71
DOI https://doi.org/10.2147/IJN.S118328
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Linlin Sun
Yi Zheng,1,* Jinbao Qin,2,* Xin Wang,2,* Zhiyou Peng,2 Peiyong Hou,1 Xinwu Lu2,3
1Department of General Surgery, The Fourth Affiliated Hospital of Guangxi Medical University, Guangxi, 2Department of Vascular Surgery, School of Medicine, Shanghai Ninth People's Hospital Affiliated to Shanghai JiaoTong University, 3Vascular Center of Shanghai JiaoTong University, Shanghai, People's Republic of China
*These authors contributed equally to this work
Background: Transplantation of allogeneic adipose-derived regenerative cells (ADRCs) is a promising treatment modality for severe ischemic diseases. However, minimal information is available on the in vivo effects, fate, and migration of ADRCs, as well as the mechanisms of their therapeutic angiogenesis.
Materials and methods: In this study, green fluorescent protein-expressing ADRCs (GFP-ADRCs) were obtained, labeled with acetylated 3-aminopropyltrimethoxysilane (APTS)-coated iron oxide nanoparticles (APTS NPs), and injected into an old apolipoprotein E knockout (ApoE-KO) mouse model with hind limb ischemia. Then, 3.0 T magnetic resonance imaging (MRI) was performed to dynamically trace the role of ADRCs targeting hind limb ischemia in the ApoE-KO mice model.
Results: Labeled cells were visualized as large hypointense spots in ischemic muscles by serial 3.0 T MRI scans during a 4-week follow-up. The presence of labeled GFP-ADRCs was confirmed by Prussian blue staining and fluorescence microscopy on postmortem specimens.
Conclusion: This study showed that allogeneic ADRCs offer great potential application for therapeutic angiogenesis in severe ischemic disease based on the efficacy and feasibility of ADRC transplantation and on the available amounts of tissue.
Keywords: allogeneic adipose-derived stem cells, cell tracking, APTS nanoparticles, hind limb ischemia, ApoE knockout mouse
Peripheral arterial occlusive disease (PAD) caused by atherosclerosis is becoming a critical public health problem in developed and developing countries.1,2 Hind limb ulceration and gangrene caused by progression of tissue hypoperfusion occur in the late stages of total occlusive peripheral vascular disease. Unfortunately, amputation is needed in more than a third of patients suffering from very severe PAD.3,4 Rapid and efficient revascularization of ischemic limb is significant to restore the function of lower limbs.5
Stem cells demonstrate tremendous potential to stimulate differentiation of various tissues, such as ischemic lower limb,6 cardiac muscle,7 nerve,8 and bones.9 Recent reports have shown that adipose tissues could supply abundant adipose-derived regenerative cells (ADRCs), which are pluripotent stem cells that can self-renew and differentiate into various cell types and can regenerate damaged tissues and organs.10–12 Thus, ADRCs offer great potential applications in regenerative medicine. However, the mechanism by which implanted ADRCs regenerate angiogenesis in ischemic tissues is unclear.
To evaluate the effects of stem cell-based therapies that are used to repair ischemic lower limbs, we must noninvasively detect the location, migration, and long-term fate of implanted cells.13,14 This goal can be achieved through magnetic resonance imaging (MRI) of the transplanted cells labeled with magnetically visible nanoparticles.15,16 The superiority of MRI in tracking and monitoring transplanted stem cells has been established using different cell types, such as bone mesenchymal stem cells (BMSCs), peripheral blood stem cells, and embryonic stem cells.17 Superparamagnetic iron oxide (SPIO) nanoparticles are the most sensitive MRI contrast agents used in cell labeling. They are safe and biodegradable, and they do not affect the proliferation and differentiation capacity of implanted cells in vitro and in vivo.18–20 However, minimal information is available on the outcome and therapeutic capacity of ADRCs labeled with magnetic 3-aminopropyltrimethoxysilane (APTS)-coated iron oxide nanoparticles (APTS NPs) and in the absence of transfection agents.
Thus, the present study aimed to test the feasibility and efficacy of ADRCs labeled with APTS NPs and to evaluate cellular imaging of cell viability, distribution, and fate of labeled ADRCs transplanted into apolipoprotein E knockout (ApoE-KO) mouse model with ischemic limbs. We specially examined whether the transplanted ADRCs could regenerate collateral vessel formation over a long period of time.
Materials and methods
Isolation of mouse ADRCs
ADRC cultures were prepared according to a reported protocol.21 Briefly, ADRCs were obtained from inguinal fat pads of green fluorescent protein (GFP)-transgenic mice with C57BL/6J background (n=30) under sterile conditions as described previously.21 Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum and antibiotic/antimycotic solution (Thermo Fisher, Carlsbad, CA, USA) was used.
On day 7, the expression profile of P3 attaching cell surface marker was evaluated by fluorescence activated cell sorting (FACS). Cells (5×105) were incubated for 30 minutes at 4°C with monoclonal antibody specific for mouse cluster of differentiation (PECAM-1 or CD31, CD34, CD90, CD105, and MHC-II (Biomedical Technology Inc.) or with unstained control for FACS Calibur analysis using FlowJo software (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).
ADRC culture and labeling
After 24 hours of incubation, the ADRCs were labeled with APTS NPs (25 μg/mL, a protocol with known safety and efficacy22) for 20 hours. The control ADRCs were incubated with DMEM. The labeling efficiency of ADRCs was determined by Prussian blue staining. After 1, 3, 5, and 7 days of culturing, cell viabilities were assessed by Cell Counting Kit-8 (CCK8; Dojindo Molecular Technologies, Japan). Absorbance measurements of the labeled ADRCs were compared with those of unlabeled ADRCs and were analyzed thrice. Moreover, the viability and apoptosis of ADRCs after labeling with APTS NPs were determined by 7 aminoactinomycin D (7AAD) and Annexin V staining and then analyzed by flow cytometry (BD Biosciences, Rockville, MD, USA).
Cell differentiation
The capability of APTS NP-labeled and -unlabeled ADRCs to differentiate into adipocytes and osteoblasts was investigated as follows. Approximately 4×105 ADRCs per well were plated in a six-well plate and then cultured for 48 hours. Three cycles of induction with adipogenic and osteoblast induction medium (Cambrex) were added to the six-well plate to stimulate differentiation of adipocytes and osteoblasts. After 3 weeks, the medium was gently suctioned, and the cells were fixed in 4% formalin and stained with Oil Red O and alizarin red. Accordingly, expressions of the osteogenic representative gene (Runx2) and the adipogenic representative gene (PPARr) were analyzed using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The cDNAs were analyzed using the following primers: Runx2, 5′ GGA CTG TGG TTA CCG TCA T 3′ and 5′ GGA GGA TTT GTG AAG ACT GTT 3′ and PPARr, 5′ CAC TCG CAT TCC TTT GAC AT 3′ and 5′ TTG ATC GCA CTT TGG TAT TCT 3′.
To investigate the role of paracrine factors regulated upon ADRC activation, we obtained conditioned medium from the labeled and unlabeled ADRCs at days 1, 2, and 3. Concentrations of mouse bFGF, SDF-1, VEGF, and HGF proteins (R&D Systems, Minneapolis, MN, USA) in the conditioned media were tested by enzyme linked immunosorbent assay (ELISA) following the manufacturer's instruction.
In vivo cell delivery and MRI
All procedures were performed on 30- to 40-week-old female ApoE-KO mice (Shanghai Biomodel Organism Science & Technology Development Co. Ltd., Shanghai, People's Republic of China; n=18) according to the guidelines prescribed by the Animal Experiment and Care Committee of Shanghai JiaoTong University, School of Medicine. All animal protocols were approved by the Animal Experiment and Care Committee of Shanghai JiaoTong University, School of Medicine. The mice were anesthetized via isoflurane (2%–3%) inhalation. To investigate the relationship of persistence of MRI signal to cell survival and engraftment, we injected ~1×106 labeled and unlabeled ADRCs in 100 μL of phosphate buffer saline (PBS) into the right ischemic hind limbs of the mice. The 3.0 T MRI scans were obtained 1, 7, 14, 21, and 28 days after cell engraftment. At least three consecutive short-axis slices were obtained to confirm the area of cell injection. MR images were analyzed by ImageJ software (Bethesda, MD, USA). Signal intensity in the hind limb was also determined. Laser Doppler blood perfusion imagers (Omegaflow FLO-CI; Omegawave, Portland, OR, USA) were performed to measure the cutaneous blood flow at 4 weeks after transplantation.
Histology and immunohistochemistry
The mice were euthanized 4 weeks after ADRC transplantation. Both hind limbs were embedded in paraffin and cut into 5 μm sections. Hematoxylin and eosin (H&E) staining and Prussian blue staining were performed to identify the fate of the transplanted ADRCs. For iron particle staining, sections were incubated with Perls reagent in the dark for 30 minutes and then counterstained with fast red.
For histological examination via immunofluorescence, 5 μm frozen sections were cut. For immunostaining, sections were incubated with primary antibodies, rabbit anti-mouse CD31 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), followed by incubation with secondary antibody, APC-conjugated mouse anti-mouse IgG (BD Pharmingen, NY, USA), and Alex 555-conjugated goat anti-rabbit IgG (Thermo Fisher), and then counterstained with 4′,6-diamidino-2-phenylindole (DAPI).
Lungs, liver, and spleen were collected, fixed, and embedded in paraffin. Serial sections were stained with H&E and iron staining to detect iron particles for evaluation of morphological changes. Controls were also established in the unlabeled groups.
Data are expressed as mean ± standard error of the mean. Statistical significance was evaluated using unpaired Student's t-test for comparison between two groups. Values of P<0.05 (*) denoted statistical significance.
Characterization of cultured ADRCs
P3 ADRCs are fibroblast-like cells in terms of shape and were culture expandable every other day (Figure 1A). The ADRCs obtained from GFP-transgenic mice continuously expressed Luc-eGFP reporter gene, which are shown green in Figure 1B. In addition, the Luc-eGFP activity did not change significantly in in vitro ADRC culture (data not shown).
FACS analysis showed that P3 ADRCs were positive for stem cell marker CD90 (59.7%±0.6%) and CD105 (76.5%±0.8%) but negative for 1.3%±0.1% CD31 (a marker of endothelial cells), 1.2%±0.1% CD34 (hematopoietic and endothelial progenitor cell marker) cells, and 0.9%±0.1% MHC-II (Figure 1).
ADRC labeling and cytotoxicity
ADRCs were labeled with APTS NPs and tested for labeling efficiency and cell viability. Microscopic observation revealed APTS NPs in the cytoplasm. In Figure 2A, ~100% of the ADRCs incorporated APTS NPs and are stained blue after 24 hours of culturing. The unlabeled ADRCs showed no blue staining particles in the cytoplasm (Figure 2B). CCK8 assay showed that viability of ADRCs remained at >95% after APTS NP labeling for at least 7 days (Figure 3A), and no increase in apoptosis was observed on day 3 as shown by FACS analysis (Figure 3B). Moreover, no difference in the Annexin rates was observed between APTS NP-labeled (Figure 3C) and APTS NP-unlabeled ADRCs (Figure 3B).
Iron labeling of ADRCs with 25 μg/mL APTS NPs did not increase cell necrosis nor apoptosis (P=0.530) as revealed by flow cytometry (Figure 3B and C). Therefore, 25 μg/mL APTS NPs was suitable for the in vivo experiments.
Cell differentiation
ADRCs could differentiate into mature adipocytes and osteoblasts in appropriate concentrations of adipocytes and osteoblasts differentiation media. In in vitro culture experiments, differentiation of APTS NP-labeled and -unlabeled ADRCs into adipocyte lineages was identified through microscopic observation of intracellular red lipid droplets and positive reaction to Oil Red O (Figure 4A and B) and qRT-PCR of Runx2 (Figure 4C). Moreover, differentiation of APTS NP-labeled and -unlabeled ADRCs into the osteoblast was confirmed through alizarin red staining, and the result demonstrated that ADRCs could differentiate into calcium nodules (Figure 4D and E). The qRT-PCR of the relative adipocytes and osteoblasts gene expression levels in APTS NP-labeled groups showed no significant difference in the transcript levels of Runx2 (Figure 4C) and PPARr (Figure 4F) compared with the ADRCs control groups.
Angiogenic cytokine production by ADRCs
To prove that ADRCs secrete a certain amount of paracrine factors, including bFGF, SDF-1, VEGF, and HGF proteins, the conditioned culture medium analyzed by using ELISA. Concentrations of bFGF, SDF-1, VEGF, and HGF in conditioned medium showed no significant difference between the labeled and unlabeled ADRCs (Figure 5).
In vivo tracking of ADRCs
The mice were scanned on days 1, 7, 14, 21, and 28 to investigate the migration of the engrafted cells in hind limb. The 3.0 T MR images are shown in Figure 6. ADRCs could be clearly identified in the adductor muscles, and their migration could be monitored for at least 4 weeks. The hypointensities ("black spots") were found in the region of ADRC injection in all the mice transplanted with APTS NP-labeled ADRCs (Figure 6A). In the cross section of the hind limb of the ApoE-KO mouse models 1 week after treatment, the site of injection exhibited wide hypointensities, which extended beyond the actual location of the labeled ADRCs ("blooming effect"), in addition, the hypointensities decreased with time. Control-unlabeled ADRCs or saline-injected groups showed no hypointensities in their muscles as revealed by MRI (Figure 6A). Four weeks after cell transplantation, black spots could still be observed under MRI (Figure 6A). Control animals injected with unlabeled cells expectedly showed no hypointensities MR signals, as expected. The data of the MRI signal values in the APTS NP-labeled groups were significantly (P<0.05) lower compared with the unlabeled ADRCs groups and gradually increased to normal levels at 4 weeks (Figure 6B). Representative color laser Doppler images of superficial blood flow in lower ischemic limbs before and after treatment are shown in Figure S1. We found that the superficial blood flow was higher after the APTS NP-labeled ADRC treatment than before the treatment (Figure S1).
Location of implanted ADRCs
We examined whether transplanted ADRCs could survive and differentiate into endothelial cells (ECs) in vivo. Immunofluorescence staining showed that some of the GFP-positive cells were retained near vascular structures and capillaries stained with CD31 (Figure 7A–C) and some of the ADRCs were also GFP positive. Quantitative analysis demonstrated that capillary density in ischemic hind limb muscles was significantly greater in the APTS NP-labeled and -unlabeled ADRCs groups than in the control groups (P<0.05 versus control; Figure 7D). Our findings indicated that the transplanted ADRCs already differentiated into the endothelial cells and possibly contributed to vascular formation to a certain extent. Furthermore, no tumors were formed in transplanted mouse after at least 30 days (data not shown).
Histological analysis
ADRCs were particularly visualized in the ischemic zone of the hind limb after Prussian blue staining (Figure 8). No Prussian blue-positive cells were localized in the ischemic hind limb of the unlabeled group (Figure 8).
To further investigate whether the remaining APTS NPs could accumulate in different target organs, the liver, lungs, and spleens of the mouse models were collected and then stained them with H&E and Prussian blue staining. Prussian blue-positive staining, which represents iron, presumably from APTS NP labeling of the ADRCs, was detected in the liver, spleen, and lung (Figure 8). The results showed that APTS NPs did not accumulate in the lungs of any of the mouse models. However, a small amount of APTS NPs was found in livers and spleens from APTS NP groups of mouse models.
The present study found that 1) P3 ADRCs expressed mesenchymal stem cell markers and were negative for endothelial lineage markers (CD31) in vitro. 2) P3 ADRCs could be efficiently and safely labeled with APTS NPs. 3) Direct local implantation of APTS NP-labeled ADRCs into ischemic hind limb muscles could be monitored by 3.0 T MRI and can improve neovascularization.
Adipose tissues comprise two main cell types, namely, adipocytes and ADRCs.23 Studies have proven that ADRCs contain multipotent mesenchymal stem cells that can differentiate into various cell types. Thus, they offer great potential applications in regenerative medicine.24 MRI is a highly superior technique in tracking and monitoring transplanted stem cells labeled with contrast agents (such as SPIO).25 However, Kostura et al26 reported that chondrogenic differentiation of BMSCs but not adipogenesis could be impaired. In this study, APTS NPs were used instead of SPIO and found no significant difference in the differentiation potential between APTS-labeled and -unlabeled ADRCs. APTS NPs do not require addition of some transfection reagents to enhance the labeling efficiency compared with SPIO, which may inhibit stem cell differentiation ability into chondrocytes. The procedure used to label ADRCs with APTS NPs without poly-L-lysine (PLL) is highly efficient and safe, as well as more practical than that used in a previous study.27 We used a relatively low APTS NP concentration (25 μg/mL) as previously used in several trials, which have shown clear MRI visualization of ADRCs without altering the viability, apoptotic indices, survival, migration, and differentiation compared with those of their unlabeled counterparts.
Long-term observation of APTS NP-labeled ADRCs might be limited to a certain extent because of APTS dilution as ADRCs divided. To date, some studies have proven that MRI signal originating from surviving APTS NP-labeled cells may be overestimated because of phagocytosis by macrophages and that extracellular iron particles contribute to MRI signal.28,29 To address these problems, we compared the use of GFP reporter gene marker and APTS NPs in labeling ADRCs in vitro and in vivo. After double labeling, the fate of implanted ADRCs could be simultaneously monitored by MRI and immunofluorescent microscope. It was found that the relative APTS NP-labeled ADRCs can be detected for at least 4 weeks by MRI and immunofluorescent microscope.
Limited information is available on the underlying mechanisms of allogeneic ADRCs in therapeutic angiogenesis. Boden et al30 showed that ADRC transplantation improved angiogenesis resulting from secretion of angiogenic factors. Moreover, this study demonstrated that ADRC implantation significantly augmented angiogenesis not only by endothelial differentiation (VEGF, HGF, and bFGF) but also by chemokines, such as SDF-1. SDF-1 may play an important role in the ADRC-regenerated angiogenesis to a certain extent by facilitating mobilization of ADRCs.
Our study demonstrated that APTS NPs are safe and biodegradable and they do not affect the proliferation and differentiation capacity of in vitro and in vivo of cells transplanted into the ischemic hind limb. The results of this study will provide a new source of seed cells for severe ischemic diseases and bring new insights into regenerative therapies.
This research is financially supported by the National Natural Science Foundation of China (81370423, 81570432, 81601621, and 81670440) and the Natural Science Foundation of Shanghai Science and Technology Committee (grant nos 134119a2100 and 20124Y132) and is also sponsored by Shanghai Sailing Program (grant no 15YF1406900) and Shanghai JiaoTong University School of Medicine Doctoral Innovation foundation (BXJ201428).
The authors report no conflicts of interest in this work.
Stoner MC, Calligaro KD, Chaer RA, et al. Reporting standards of the Society for Vascular Surgery for endovascular treatment of chronic lower extremity peripheral artery disease. J Vasc Surg. 2016;64(1):e1–e21. | ||
Hamur H, Onk OA, Vuruskan E, et al. Determinants of chronic total occlusion in patients with peripheral arterial occlusive disease. Angiology. Epub 2016 Apr 7. | ||
Vallabhaneni R, Kalbaugh CA, Kouri A, Farber MA, Marston WA. Current accepted hemodynamic criteria for critical limb ischemia do not accurately stratify patients at high risk for limb loss. J Vasc Surg. 2016;63(1):105–112. | ||
Mutirangura P, Ruangsetakit C, Wongwanit C, et al. Enhancing limb salvage by non-mobilized peripheral blood angiogenic cell precursors therapy in patients with critical limb ischemia. J Med Assoc Thai. 2009;92(3):320–327. | ||
Dominguez A 3rd, Bahadorani J, Reeves R, Mahmud E, Patel M. Endovascular therapy for critical limb ischemia. Expert Rev Cardiovasc Ther. 2015;13(4):429–444. | ||
Mohamed Omer S, Krishna SM, Li J, Moxon JV, Nsengiyumva V, Golledge J. The efficacy of extraembryonic stem cells in improving blood flow within animal models of lower limb ischaemia. Heart. 2016;102(1):69–74. | ||
Jiang Q, Yu T, Huang K, Lu J, Zhang H, Hu S. Remote ischemic postconditioning ameliorates the mesenchymal stem cells engraftment in reperfused myocardium. PLoS One. 2016;11(1):e0146074. | ||
Wang Y, Liu H, Ma H. Intrathecally transplanting mesenchymal stem cells (MSCs) activates ERK1/2 in spinal cords of ischemia-reperfusion injury rats and improves nerve function. Med Sci Monit. 2016;22:1472–1479. | ||
Ono N, Kronenberg HM. Bone repair and stem cells. Curr Opin Genet Dev. 2016;40:103–107. | ||
Balducci L, Alessandri G. Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens. Methods Mol Biol. 2016;1416:259–274. | ||
Legzdina D, Romanauska A, Nikulshin S, Kozlovska T, Berzins U. Characterization of senescence of culture-expanded human adipose-derived mesenchymal stem cells. Int J Stem Cells. 2016;9(1):124–136. | ||
Kim JH, Park Y, Jung Y, Kim SH, Kim SH. Combinatorial therapy with three-dimensionally cultured adipose-derived stromal cells and self-assembling peptides to enhance angiogenesis and preserve cardiac function in infarcted hearts. J Tissue Eng Regen Med. Epub 2016 Jun 3. | ||
Wolfs E, Verfaillie CM, Van Laere K, Deroose CM. Radiolabeling strategies for radionuclide imaging of stem cells. Stem Cell Rev. 2015;11(2):254–274. | ||
Rodriguez-Porcel M. In vivo imaging and monitoring of transplanted stem cells: clinical applications. Curr Cardiol Rep. 2010;12(1):51–58. | ||
Ariza de Schellenberger A, Kratz H, Farr TD, et al. Labeling of mesenchymal stem cells for MRI with single-cell sensitivity. Int J Nanomedicine. 2016;11:1517–1535. | ||
Elhami E, Dietz B, Xiang B, et al. Assessment of three techniques for delivering stem cells to the heart using PET and MR imaging. EJNMMI Res. 2013;3(1):72. | ||
Kalber TL, Ordidge KL, Southern P, et al. Hyperthermia treatment of tumors by mesenchymal stem cell-delivered superparamagnetic iron oxide nanoparticles. Int J Nanomedicine. 2016;11:1973–1983. | ||
Qin J, Li K, Peng C, et al. MRI of iron oxide nanoparticle-labeled ADSCs in a model of hindlimb ischemia. Biomaterials. 2013;34(21):4914–4925. | ||
Busato A, Bonafede R, Bontempi P, et al. Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes. Int J Nanomedicine. 2016;11:2481–2490. | ||
Skelton RJ, Khoja S, Almeida S, et al. Magnetic resonance imaging of iron oxide-labeled human embryonic stem cell-derived cardiac progenitors. Stem Cells Transl Med. 2016;5(1):67–74. | ||
Qin JB, Li KA, Li XX, et al. Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury. Int J Nanomedicine. 2012;7:5191–5203. | ||
Li XX, Li KA, Qin JB, et al. In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia. Int J Nanomedicine. 2013;8:1063–1073. | ||
Comella K, Parcero J, Bansal H, et al. Effects of the intramyocardial implantation of stromal vascular fraction in patients with chronic ischemic cardiomyopathy. J Transl Med. 2016;14(1):158. | ||
Guo LZ, Kim TH, Han S, Kim SW. Angio-vasculogenic properties of endothelial-induced mesenchymal stem cells derived from human adipose tissue. Circ J. 2016;80(4):998–1007. | ||
Patsula V, Kosinova L, Lovric M, et al. Superparamagnetic Fe3O4 nanoparticles: synthesis by thermal decomposition of iron(III) glucuronate and application in magnetic resonance imaging. ACS Appl Mater Interfaces. 2016;8(11):7238–7247. | ||
Kostura L, Kraitchman DL, Mackay AM, Pittenger MF, Bulte JW. Feridex labeling of mesenchymal stem cells inhibits chondrogenesis but not adipogenesis or osteogenesis. NMR Biomed. 2004;17(7):513–517. | ||
Mishra SK, Kumar BS, Khushu S, Tripathi RP, Gangenahalli G. Increased transverse relaxivity in ultrasmall superparamagnetic iron oxide nanoparticles used as MRI contrast agent for biomedical imaging. Contrast Media Mol Imaging. Epub 2016 May 27. | ||
Raynal I, Prigent P, Peyramaure S, Najid A, Rebuzzi C, Corot C. Macrophage endocytosis of superparamagnetic iron oxide nanoparticles: mechanisms and comparison of ferumoxides and ferumoxtran-10. Invest Radiol. 2004;39(1):56–63. | ||
Terrovitis J, Stuber M, Youssef A, et al. Magnetic resonance imaging overestimates ferumoxide-labeled stem cell survival after transplantation in the heart. Circulation. 2008;117(12):1555–1562. | ||
Boden J, Lassance-Soares RM, Wang H, et al. Vascular regeneration in ischemic hindlimb by adeno-associated virus expressing conditionally silenced vascular endothelial growth factor. J Am Heart Assoc. 2016;5(6):e001815. |
Supplementary material
Figure S1 Representative color laser Doppler images of superficial blood flow in lower ischemic limbs before and after treatment. |
© 2016 The Author(s). This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.


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Web scraping is the process of automatically extracting data and collecting information from the web.
It could be described as a way of replacing the time-consuming, often tedious exercise of manually copy-pasting website information into a document with a method that is quick, scalable and automated. Web scraping enables you to collect larger amounts of data from one or various websites faster.
It's possible to do web scraping with many programming languages.
However, one of the most popular approaches is to use Python and the Beautiful Soup library
How does it work?
When we scrape the web, we write code that sends a request to the server that's hosting the page we specified. The server will return the source code — HTML, mostly — for the page (or pages) we requested.
So far, we're essentially doing the same thing a web browser does — sending a server request with a specific URL and asking the server to return the code for that page.
But unlike a web browser, our web scraping code won't interpret the page's source code and display the page visually. Instead, we'll write some custom code that filters through the page's source code looking for specific elements we've specified, and extracting whatever content we've instructed it to extract.
Is it Legal?
Unfortunately, there's not a cut-and-dry answer here. Many websites don't offer any clear guidance one way or the other. Some websites explicitly allow web scraping. Others explicitly forbid it.
Before scraping any website, we should look for a terms and conditions page to see if there are explicit rules about scraping.
We can also check the robots.txt file for any website to see which pages are permitted to be scraped and which pages disallow scraping. Ex: https://www.facebook.com/robots.txt. Here, Facebook clearly specifies which pages are allowed to be accessed and which pages are not.
The first thing we'll need to do to scrape a web page is to download the page. We can download pages using the Python requests library.
The requests library will make a GET request to a web server, which will download the HTML contents of a given web page for us.
We can use the BeautifulSoup library to parse the web page, and extract the text.
We first have to import the library, and create an instance of the BeautifulSoup class to parse the web page.
Then, We can print out the HTML content of the page using various BeautifulSoup methods.
Let's scrape the content from YouTube's robot.txt file.
First, we have to import requests library and send a GET request to download data from the desired location.
import requests
request = requests.get('https://www.youtube.com/robots.txt')
Now, we have to import BeautifulSoup to parse the web page and print all the data that we have access to.
from bs4 import BeautifulSoup
data = BeautifulSoup(request.text, 'html.parser')
Now, we can read the output and find out which pages YouTube allows to be scraped and which it does not


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Ghanaian Lady Reveals Her Baby Was Switched At Birth In Canada
- A young mother of four has recently shared that the son she gave birth to was swapped with another baby
- In an interview on Starr103.5 FM she revealed that she noticed her child had different features and that got her worried
- She received a call months later from the hospital in Canada she gave birth at and was informed about the switch
PAY ATTENTION: Click "See First" under the "Following" tab to see YEN.com.gh News on your News Feed!
A Ghanaian lady has recently taken to social media to open up about how her baby was switched at birth at a hospital in Canada.
In an interview on the YouTube channel of Starr103.5FM, the mother of four known as Giselle revealed she travelled to Canada to give birth to her son and upon delivery, her son was diagnozed with jaundice hence had to be taken to the ICU.
She moved back to Ghana and after six months noticed the unfamiliar features in the child.
According to Giselle, she had not had extra marital affair and was very confused about what was on-going and her husband was also torn.
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Few months later, the hospital in Canada reached out to her and confirmed the switch and requested she report to the hospital.
Giselle revealed she finally got her actual child back when he turned one and half years.
She also added that bonding with her child has been very difficult but she is hoping for things to get better.
Giselle took the opportunity to advise Ghanaians to be considerate on women whenever there is DNA mismatch as it could have been a switch at birth.
The young mother shared more of her story in the video switched below;
Man Discovers 7-Year-Old Child Is Not His After Doing DNA Test, Voices Out Deep Frustration
Meanwhile, YEN.com.gh earlier reported that a man, Eguveso Enifome, went online to cry out after a 7-year-old child said to be his was found out not to be so after a DNA test.
Enifome in one of the several videos he shared on his Facebook page, however, admitted that he was intimate with the woman before she got pregnant.
He said he was shocked when he was informed after the baby was two years old that the kid is his.
Our manifesto: This is what YEN.com.gh believes in
Source: YEN.com.gh


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Kashi Vishwanath Gange
These four verses are from a prayer called the Shiva Stuti from the Ramacharitamanasa (Ramayana) of Tulsi Das.
The story of Rama incarnation and lilas is being told by a crow to Garuda, the King of birds. The crow Kakabhusundi is repeating the story that Shiva had told Parvati.
Garuda asks the crow how he has come to know the story and how he gained so much wisdom. Kakabhusundi tells his life story. He was an arrogant disciple of a very loving guru. No matter how much his guru loved him, he just didn't get it. One day he was meditating and his guru walked in the room. He knew it was his guru but still he didn't get up. At that instatnt Shiva himself manifested out of the Lingam and cursed him to become a crow and undergo millions of births of torture and hell for disrespecting his guru. His guru was so upset at the curse that he prayed to Shiva to rescind it. This is that prayer.
Shiva was so moved by the prayer that even though he could not rescind the curse he added to it, saying that even though the disciple would remain a crow he would become the foremost knower of God in the universe and would be able to see all the lilas(Play) of the Lord in all His manifestations in all the universes.
Namaameesha meeshaan nirwaana roopam
Wibhum vyaapakam braham wedaswaroopam
I bow to the Lord of the Northwest(Shiva)
Whose form is liberation itself.
The supreme Brahman, in the form of the Vedas,
Spread out over the whole Universe.
Nijam nirgunam nirwikalpam nireeham
Chidaakaasha maakaasha waasam bhajeham
You are without attributes and beyond time,
without any limitation; beyond desire.
You are the sky of bliss. I worship you.
Niraakaara monkaara moolam tureeyam
Giraa gyaana goteeta meesham gireesham
You are the formless One
beyond the Omkar which has it root in you.
You are beyond words, knowledge and the physical world,
Oh Lord of Mountains.
Karaalam mahaakaala kaalam kripaalam
Gunaagaara sansaara paaram natoham
You are the terrifying One who devours time;
which devours everything.
You are the most compassionate one.
All the qualities and attributes of experience,
originate in you,
But the world can never find your end.
Hara Hara Mahaadeva Shaambho(Bom Bom)
Kaashi Vishwaanaatha Gange
I call the names of The Great Lord Shiva
Who lives on the Banks of the Ganges at Kashi(Benares)
I sit on the banks of the river of life,
watching children being born, seeing how they grow,
how they take on the lives they have been given,
how they bend and wither with the weight of this world,
how those lives
disappear back into the Earth and Sky.
I breathe into them as they come
and they breathe into me as they leave.
I am the Great Lord in whom all find rest
In their true nature, my Self.
Shivoham- I am Shiva!
Appreciations ~
My life is the gift of my Guru, Neem Karoli Baba. If there is anything of valueon this CD, it is his. It is his blessings that flow through me. My job is to get out of the way so they can flow unobstructed, like a river into the ocean. Unfortunately, still being a work in progress, I am like a sandbar, gradually being worn away by the current of Grace. Please accept these chants in that spirit.
- To Shri Siddhi Ma and Shri Jiwanti Ma
- To all the Great Ones, known and unknown who have shed their light on the Path and given us the strength to follow
- To the elder devotees of my Guru, who took me in and taught me the essence of devotion and surrender;
- To 'AH', Heart of my Heart;
- To Ma And Baba(Mr& Mrs K.C. Tewari) my Indian parents, whose presence is with me everyday
- To my physical parents
- To Dada, "Isn't is that?"
- To Ram Dass-For still being here;
- To Wah for keeping us on the road
- Rick, "You gotta do 'Live."
- To the East and West Coast "Chapters" of the Ever Changing Kosmic Kirtan Posse for the devotion and committment to the chanting for the Love of Love Alone


text:
News Post
Origin and Cause Welcomes Andrea Gaynor
June 1, 2022
Origin and Cause, Canada's largest forensic engineering and fire investigation firm, is pleased to welcome Andrea Gaynor to the Fire and Explosion Investigation team in Ancaster, Ontario.
Andrea is a Certified Fire and Explosion Investigator. She has over 10 years of experience with the Ontario Fire Marshall, prior to which she served as an officer with the Halton Regional Police Service for 22 years. To date Andrea has been involved in over 350 fire and explosive investigations. She has been qualified as an expert in fire and explosive investigations in Ontario Court and Superior Court of Justice for the Province of Ontario. Andrea is also an associate instructor for the Ontario Fire College and has taught the NFPA 1033 fire course to firefighters and police.
"Andrea has had a long and decorated career in emergency services. She served with the Halton Regional Police for over 20 years and has spent the last decade with the Ontario Fire Marshall," said Art Beleda, Branch Manager. "Her experience and talent add tremendous value to our team, and we couldn't be happier to have her aboard."
For more information, please contact:
Art Beleda, Branch Manager and Fire & Explosion Investigator
(905) 648-5522
Andrea Gaynir, Fire & Explosion Investigator
(519) 369-7163


text:
Karmic and Past Life Reading (written from-scratch report)
Karmic and Past Life Reading (written from-scratch report)
This is a past life and karmic reading that looks at both your natal chart and your draconic chart. From this you will get an idea of where you come from in terms of your most recent previous lifetime and what you wanted to accomplish this time around. You will also see how the chart of your soul across all incarnations shines through in this lifetime.
These are done from scratch, so the turn around time is usually about a week from when you order.


text:
OTAY MESA, Calif. (CNS) - No one was injured Monday when three individuals were rescued from atop a U.S.-Mexico border wall in the Otay Mesa area.
Firefighters responded to a U.S. Customs and Border Protection backup for three individuals who were stuck at the top of the border wall near Britannia Boulevard and Britannia Court around 7:30 a.m., according to the San Diego Fire and Rescue Department.
Border Patrol agents confirmed the three people were detained and awaiting processing.


text:
What is the Better Chat Mute plugin?
Better Chat Mute (BCM) is a RUST plugin that provides the functionality to permanently mute players in text chat within RUST. Currently updated and supported by LaserHydra, BCM is a free-to-use plugin available for download on uMod. It can act as either a compliment to other Better Chat mods or as its own standalone tool.
Potential reasons to use the Better Chat Mute plugin
Better chat mute goes beyond the standard mute function provided by the default RUST client. When muted with this mod installed, a database record is saved within your RUST server folders, and players are permanently muted (with or without reason listed). Users muted with this plugin can no longer transmit to global chat; only admins can read their team chat. Please check out our Better Chat Mute Voice Plugin guide to extend this functionality to voice chat.
Installing the Better Chat Mute plugin
As with all uMod plugins, the server you intend to install them on requires uMod (Oxide) to be installed to facilitate their function. For a step-by-step perspective on how to install this plugin, please reference the following list:
- First, you must have uMod/Oxide installed on your server. Use our Installing uMod (Oxide) on Your RUST server guide if you need help; otherwise, if you use a third-party hosting, their user interface will have instructions on how to get started. It may be tooled explicitly to their platform, so reach out to your host for details.
- Acquire the latest version of the plugin from uMod's Better Chat Mute page, or download the CS file directly.
- Locate the downloaded
file and install the plugin on your RUST server, or follow your host-specific instructions. - Once Better Chat Mute is installed in the plugins folder, and if the server is actively running, uMod will immediately create an alternate
file in your config folder. It is essential to differentiate these files and not mistake them, as you must make configuration changes in the .json file in the config folder.
Additional dependencies
Like many other uMod plugins, the RUST Better Chat Mute plugin may work as a standalone mod or in conjunction with other select mods.
A full list of such mods on uMod includes:
- Better Chat
- Better Chat Filter
- Better Chat Mute Voice
- Chat Translator
- Coloured Chat
- Discord Chat
- Discord Logger
- Discord Messages
- GST Report
- Player Administration
- Rustcord
As with all plugins that you might try to install, Better Chat Mute plugin has its own small list of permissions. Make sure you reference our Oxide Permissions guide to understand the oxide.grant
permission system better.
Specific permission command lines include:
Command | Description |
betterchatmute.use | Necessary to issue the mute command, and for general use |
betterchatmute.use.global | Necessary for the toggleglobalmute command usage |
betterchatmute.permanent | Necessary for the use of the permanent muting feature |
Activation steps
Once installed and permitted to use the mod, players may activate additional commands as follows: (preface with /
if using in chat)
Command | Description |
mute 'player name or steamid' [reason] | Mute specified player permanently |
mute 'player name or steamid' time: 1d1h1m1s [reason] | Mute player for specified time |
mutelist | Display players that are muted |
toggleglobalmute | Mute chat for every player not given the betterchatmute.use.global permission |
unmute 'player name or steamid' | Remove mute from specified player |
How to update the Better Chat Mute plugin
Updating the RUST Better Chat Mute plugin is simple and quick.
- Visit the uMod Better Chat Mute plugin page.
- Download the latest version of the Better Chat Mute plugin
- Overwrite the existing
file in your Oxide plugins folder.
It is best practice not to uninstall the mod, as you do not need to alter the JSON file. It will remain with your existing settings (unless the developer otherwise suggests a complete reinstall).
How to remove the Better Chat Mute plugin
As the RUST Better Chat Mute plugin doesn't impact player experience or game features necessary to run processes in the game itself, removing the plugin is as simple as deleting BetterChatMute.cs
from your plugins folder.
A more thorough approach for removing Better Chat Mute is to:
- Prepare and properly shut down the RUST server
- Open the Oxide plugins folder in your server directory
- Locate and delete the
file - Open the Oxide config folder in your server directory
- Locate and delete the
configuration file - Restart the RUST server
Suppose you want to disable the Better Chat Mute plugin for a short period. You can use the oxide.unload BetterChatMute
command, which you may also run in the F1 console, RCON, or Web Console.
Disclaimer and seeking assistance
Corrosion Hour does not maintain the RUST Better Chat Mute plugin, nor do we assist with modifying the plugin outside the config scope. Visit the plugin's uMod page directly to support the plugin creators, report bugs, or propose feature requests. Please get in touch with an admin in our Discord community for help with the steps and instructions in this guide.


text:
We're into November and usually I'll write something reminding you about gratitude. And that's important. So, remember the small stuff for which you're grateful.
Got it?
Okay, with that practice completed, let's look at something really absurd…
We plan for so much. We've planned for the holidays. We know where we're going to be at the end of the month. We know with whom we're getting together next month. You might have already planned your menu. You might have had that planned a month or two ago.
And chances are, you've also begun planning where you anticipate stress.
Yep, I know it happens to a lot of us at this time of year. We inventory who needs what from us. We also look at whom we'll share a table and/or conversation. Who is going to say something offensive and then what will happen when they're put back in line? Drama. We're planning for drama!
What if you did it...
Are we really in a contest to focus on bad news?
It sure seems like that. Doesn't it?
There's a hidden danger in doing so. (uh-oh, more bad news?)
No, this is to keep you aware. The bad news about the bad news is that you train your subconscious to focus on bigger, "badder," worse bad news.
Did you hear about the floods?
Yeah, and the economy?
And the…
How much worse could it get?
It depends on how much drama you want to let into your life.
Floods? Yes, we had rain.
Economy? Yes, it's a reset given where we were. We're seeing a new economy based on The Great Resignation culture we've become a part of.
What else? Shootings, racism, political gaming…?
What will you let into your psyche? When you focus on what could be worse, you'll find the answer. In doing so, you put your limbic system into the "on alert" space.
What if… what if you chose to offer kind...
You've changed, and it's not just that cute Halloween costume you're wearing. Nope, you've changed. There's a question, though…
Do you like where you're heading? Even though some of your predilections might be similar now to your past, what you like – essentially, what you value – has changed over time. And so the question of whether you like where you're heading demands something of you: being deliberate.
Since the seeds we plant now become the blooms/trees/fruit of tomorrow, you can consciously choose what your tomorrow looks like. Do you live in fear and doubt? What would happen if you lived your life feeling just that bit more certain? Are you someone who views the world (your world) from a place of lack? How might your life be different if you chose to simply live in a space of gratitude for the small stuff (it is Thanks-giving month, after all).
You've changed. And you're supposed to!
Do you LOVE what you do?
I do! I absolutely love what I do. It's an obsession to learn, synthesize my reading, give thought to it, and create something that's uniquely my voice.
How might you share something of yours out in the world, with your family, or your work team?
Passion creates trust.
If it's genuine, people know it. They tell me that all the time. It's not some put on enthusiasm. People who work with me, especially recently, are commenting on my authenticity. What's weird about writing about it is that I have to look objectively at my path.
Over the past couple of years, I've worked really hard to let go of a lot of "supposed to" stuff. You'd think that an adult at my age (yes, I just had another birthday last week) might have it all together. And I think it's finally safe to share that you can have it all together and still be in a state of growth so that you actually never...
The mind, once stretched by a new idea, never returns to its original dimensions.
Consider the last time you learned something new.
You really can't unlearn it. You can learn new things that modify what you know, you can build on top of it, but you can't erase having learned it.
Now, consider what it would feel like to have absolute confidence.
Think about the last time you started down a path and got derailed by doubt.
Those are two very different feelings.
You can't un-feel confidence. You can, however, be plagued by doubt. And I am here to remind you that doubt is your enemy. Doubt is a lie.
The truth is that doubt, and fear, and all of that stuff that sounds like voices in your head wondering how the heck you're going to get through… all of that has come from your early experiences that have been modified in adulthood to keep you safe.
Think you can't make that next call,...
We've stepped into the final quarter of the year and we're at a place of wrap-up or catch-up or perhaps simple overwhelm. It's time to retake control of your calendar, your projects, your finances, and your life!
We all have competing priorities. Sometimes, though, we end up putting the real priorities aside to deal with the crisis of the moment. When you're in a spin, attending to what has just grabbed your attention moment by moment, day by day, you need to step back.
You need a shift in your perspective, what I call the Magic of Parallax Perspective.
From my last five blog posts, you might have gotten that M-A-G-I-C is a framework I use for leadership and personal development.
M = Mindset
A = Accountability
G = Gratitude
I = Inspiration
C = Clarity
For more on each of those, listen into my podcast One Sharp Sword and/or go back to my blogs to read through them.
The parallax perspective is a...
Most people celebrate January 1st as the New Year.
For me, I use my birthday to celebrate.
And yes, today is my birthday!
I do some deep reflection. I look at where I've been this past year. And OH, what a year!
I look back at my accomplishments, the struggles, and the wins. I look at the course-correction I made for myself, as well. Success isn't a straight path. It's a continuous climb with multiple points of celebration. And today, I celebrate the past and the future!
I also plan for the future.
Planning is different from wishing. I have a few wishes. I don't hang on to those so much. In fact, it's my DESIRES that I pay attention to. What is my heart telling me? What path must I pay attention to and not ignore??
This past year, I've fulfilled some serious dreams and started on some new ones.
A couple of years ago, I had a strong desire to be on Big Stages. I want to… need to share my...
Just own it.
Really. You don't need permission anymore. You have influential power.
You may not think so, or maybe it doesn't seem so all the time. And yet, if you're human and in society, there's a good chance people are noticing you. And because they are, you have the opportunity to make a difference in their lives.
You don't even need to directly interact with them. Just be the model of the person you hope to be!
Are you smiling?
Practice doing that more.
Are you engaging and deliberately, actively, positively® lifting them in some way?
Practice doing that more.
Do you make eye contact?
Practice doing that more!
When I talk about being deliberately, positively, actively® uplifting, I'm saying that each of those adjectives matters.
During your day, are you deliberate?
Are you actively engaging?
And when you engage, are you positive? My mom taught me to leave a place...
You know that plastic bit at the end of a shoelace? Without that aglet, yes, it has a name, without it, your shoelace would fray. Over time, your shoelaces would turn to strands of broken threads.
Aren't you glad it's there?
There are so many small things that work for you in the world. In fact, if you looked at all of the small things that are working for you, you'd see that even the struggles you're having aren't so big. The struggles make you stronger. They force you to think differently.
Now if you choose to turn the struggles into something that you're worrying about, you've moved from struggling to suffering. Believe it or not, that is a choice. Suffering is a choice. Worrying is a choice. How you process the world around you is a choice!
In my blog from a couple of weeks ago, I talked about mindset. It really does affect everything.
If you can think about how happy you...
WE have to do something!
Yes, yes we do.
Don't we?
Mindset was the name of the game last week! Be sure to check out last week's Wednesdays With Wayne if you missed it.
Accountability is where we're heading today. You see, when we agree that something should be done, that we should do something, you've set yourself up for failure.
The big "a-ha" moments come to the leaders I work with when I explain this concept. It's why organizations fail to be profitable, why meetings tend to be repetitively deathly horrible, and why family chores end up sounding like nagging.
"We should do something" is a cry for help.
So, own it.
Let's say you saw a dam that had a little crack in it. "Oh, someone should fix that!"
Let's say you saw a wall in your house that got a small crack in it from the house settling over time. "Oh, we should patch...
Sign up below to join my newsletter and receive free training to your inbox ever week!


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" It's always beneficial taking advice from Dhoni. "
" My wife and kid always support me. "
" I have always wanted to play in the World Cup. "
" Losses are always hard to swallow, and every sportsperson has the same feeling. "
All 4 Imran Tahir Quotes about Always in picture
Topics: Always quotes


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Transform your hair into whatever style you desire with the right perm. Whether you're looking for tight curls or loose waves, there are a variety of different types of perms to choose from that can give your locks an extra boost.
From Spiral and Body Wave Perms to Spot, Root, Digital, and Volumizing Perms – each technique has its own unique benefits and applications.
Table Of Contents
Key Takeaways
- Spiral perm: Achieve tight curls with healthy hair.
- Body wave perm: Softer, natural-looking waves with added texture.
- Spot perm: Creates uniformity with curly hair strands of multiple textures.
- Root perm: Adds volume and texture to limp locks without an obvious curl pattern.
Spiral Perm
You can achieve tight curls with a spiral perm, which requires healthy hair to be effective. Depending on your hair type and length, this curl maintenance treatment is suitable for almost any hairstyle.
When considering a spiral perm, it's important to keep in mind the styling options that are available after the perming process as well as proper care instructions for maintaining voluminous locks. To get started with preserving natural bounce and keeping strands hydrated before, during, and after receiving a spiral perm, opt for healthy hair tips such as using leave-in conditioner regularly or deep conditioning treatments weekly to maintain softness while avoiding breakage from heat tools when possible.
These small steps will help ensure you have happy curls post-perm! Additionally, try out different products until you find ones that work best so your style stays looking fresh throughout the weeks without damaging your newly coiffed mane!
Body Wave Perm
Experiencing a body wave perm offers you softer, more natural-looking waves with added texture and longer-lasting blowouts. The process involves applying chemicals to the hair before wrapping it in rods and then heating them for desired results.
It is important to note that body wave perms are not as tight or long-lasting as spiral perms, but they can give a subtle boost of volume after styling. This type of perm is ideal for those who want their curls to look less defined yet still have some curl definition when styled properly.
Aftercare plays an essential role in maintaining your new style. Regular deep conditioning treatments should be done, along with using sulfate-free shampoos and conditioners specifically designed for chemically treated hair like Mielle Organics Biotin Oil.
This product helps nourish, strengthen, and protect your tresses while prolonging the life of your perm.
Body wave perms come at varying prices depending on length and thickness but typically cost around $100-$200 USD, making it one of the most affordable types out there! Whether you're looking for more texture or just want something different from straight styles without too much commitment, getting a body wave perm may be just what you need!
Spot Perm
A spot perm is perfect for creating uniformity with curly-haired strands that have multiple textures, giving your hair a soft and natural look. It involves the use of heat to create tight curls or waves on particular areas, making it ideal for short hairstyles like pixies and bobs.
The process is similar to other perms but does not involve treating the entire head. Instead, specific sections are cooled down using rollers or rods after being treated with chemicals. This allows stylists to customize each client's perm according to their needs and desired outcomes.
Spot perming provides many benefits, including curl definition without losing length and volume control in certain areas while leaving others untouched. For example, at the roots where texture can be kept intact longer than usual when compared to root perming techniques.
With proper care, such as deep conditioning treatments, regular trims, sulfate-free shampoos/conditioners, etc.
Root Perm
Transform your look with a root perm and enjoy voluminous, beautiful hair that's full of life.
Root perms are a great way to add body and texture to otherwise limp locks – without having an obvious curl pattern like other types of perms.
The process involves wrapping the mid-lengths and ends around rods before applying a chemical solution. This solution breaks down keratin in the hair shaft so it can be reset into curls or waves for added volume throughout the entire head.
This method is gentler than standard spiral or digital perms because there's less heat used on individual strands, resulting in less overall damage.
Compared with spiral perming techniques, root perm treatments create more subtle effects that can last up to three months if properly taken care of.
Post-perm care includes using sulfate-free shampoos and avoiding harsh brushing. These practices will help maintain good condition while preventing further damage during styling sessions!
Digital Perm
Unlock the perfect waves with a digital perm and let your hair flow like rolling ocean tides. Digital perming is an innovative way to get long-lasting curls that are soft yet voluminous. The process involves using machines and infrared heat, in addition to balanced chemicals, allowing for more precise control over the shape of each curl compared to traditional cold or hot methods.
If you're looking for loose natural waves, then digital perming might be right for you! It's also great if you have colored hair as it requires less maintenance than other types of perm treatments while providing longer-lasting results due to its gentler approach.
With proper care and upkeep after treatment, your look can last up to six months depending on hair length – making this one of the longest-lasting types of permanent curling available today! Whether you want big bouncy curls or smooth sleek waves, try out a digital perm today and experience all its benefits firsthand!
Volumizing Perm
A volumizing perm helps add bounce and body to your hair, giving it the texture you desire with lasting results. It's perfect for those looking to make a subtle change without compromising on volume.
Unlike traditional perms that produce tight curls, a volumizing perm uses larger rods to create loose waves or gentle curls while still adding volume throughout the hair shaft. The process involves wrapping sections of dampened hair around small or medium-sized rods and applying mild chemicals before heat is applied in order to lock in shape and set the curl pattern into place.
With regular trims every six weeks, this type of perm can last 3-6 months depending on how well you care for your tresses with proper hydration as well as using sulfate-free shampoos/conditioners like Monday Haircare Volume Shampoo along with other natural products such as Mielle Organics Biotin Oil and Samnyte Wax Stick Wigs.
Volumizing perms are a great alternative if you're seeking more tame wave patterns compared to body wave perms, which result in more defined ringlets due to their use of smaller rod sizes when creating the desired style.
However, both techniques offer an incredible opportunity to customize your look by mixing different size rod variations to achieve an even better look.
Multi-Textured Perm
Tired of the same old hairstyle? Maybe it's time to try something new, like a multi-textured perm! This technique uses varying perm rod sizes for realistic and tightly curled looks that are sure to turn heads.
Not only will you have more styling options with this look, but maintenance is also easier than ever before.
The best hair types for this type of perm are healthy virgin hair or already damaged tresses – both can benefit from extra volume and texture! To ensure your new look lasts longer, use clarifying shampoo once a week as well as regular deep conditioning treatments and hair masks.
Cost varies depending on length and desired style, so be sure to ask your stylist first before committing.
Stack Perm
Achieve a voluminous layered look with a stack perm, perfect for graduated bobs and short haircuts. Stack perms use different rod sizes to give the hair lift at the roots while keeping ends curled, creating an effortless yet stylish effect.
The process involves wrapping sections of hair around rods and then applying a chemical solution or heat depending on the desired result.
Maintenance is relatively simple: avoid overstyling using hot tools as this can damage curls; use shampoo specifically formulated for curly/wavy textures; opt for gentle conditioning treatments such as deep masks or oil treatments regularly; apply leave-in conditioners before blowdrying if necessary; consider touch-up appointments every few months if needed.
With careful care and maintenance, you can enjoy your stack perm's fabulous results!
Straight Perm
Transform your locks with a straight perm – permanently removing natural curls using an alkaline solution and heat for up to six months of smooth, effortless style.
The benefits of a straight perm are plentiful. The look is polished and sleek. It requires less maintenance than other types like spiral or multi-textured perms.
When selecting the right one, consider whether you need a hot or cold perm. Also, think about if you want to target just certain sections (partial) or all hair strands.
To keep your new 'do looking perfect after treatment, use sulfate-free shampoos specially designed for chemical processes. Glyceryl monothioglycolate is a great option as it helps maintain texture while still cleansing follicles without damage from harsh ingredients.
With the right care regimen in place, you'll enjoy your salon perfection at home every day!
Aftercare Tips for Perms
It's important to properly care for your perm after you leave the salon, in order to maintain and maximize its effects.
- Perm maintenance: Regularly use sulfate-free shampoos and conditioners that won't strip away curl definition or dry out hair. Avoid using heat styling tools as much as possible, since too much heat can damage permed hair.
- Styling options: Take advantage of products designed specifically for perms such as mousses and serums that help keep curls defined without weighing them down. Try scrunching damp strands with an absorbent towel instead of brushing or combing when styling wet hair after a perm treatment.
- Perm friendly products: Look out for natural oils such as coconut oil which nourish the scalp while adding shine to locks; it also helps protect against breakage from chemical treatments like permanent waves!
- Heat Damage Avoidance After A Perm: Use low settings on blow-dryers, curling irons, flatirons, etc. Shields should be used before applying any hot tool near newly-permed curls so they don't become overworked by direct contact with extreme temperatures.
- Protect Color Treated Hair With A Perm: If you have color-treated tresses, seek advice from experienced stylists regarding techniques that will not damage their hue. This could include avoiding tight rod sizes, cold application methods only, frequent trims if necessary, etc.
With so many perm types available, it's hard to know where to start. From spiral to stack perms, from body wave to digital, there is something for everyone. Whether you want a natural look or something more daring, perms provide a range of styling options.
Not only do they offer versatility, but also the ability to customize the perm to suit individual needs.
So, if you're looking to switch up your look, why not consider a perm? It could be just the thing to bring out your unique style.


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Downloads: Jukebox (~25 KB)
Description: Jukebox is an application that helps you make your pc a jukebox. You need WinAMP to use it. Warning: Jukebox can only be closed in Adminmode. If you didn't set a password just leave the field empty and click on Activate Adminmode to activate adminmode. You can use Jukebox on your party: Just open Jukebox.ini(will be generated in Jukebox directory after first run) and set maximized and alwaysontop to 1 and set your password. When you run Jukebox now noone without the password will be able to do anything else on your pc than choosing a song. You don't even need a keyboard.


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If you are looking for the Instapage Free Trial, it is readily available here. So lets talk about Clickfunnel Vs Instapage…
One of the greatest pains of a budding web entrepreneur's life is optimizing an efficient Landing Page. I imply it's easy to construct one, however how effective is it going to be. You never know till you look at information and statistics.
Instapage, a well known landing page editor and designer, declares to increase your conversion by up to 400% by personalizing landing pages in the most reliable way.
In the evaluation listed below, we shall see how reliable Instapage actually is.
I understand what you're believing, 40% conversion rate, on a landing page, that's ridiculous. Tip number one, usage tools like Instapage and Click Funnels. The cool part about Click Funnels and Instapage and even Lead Pages is they break down the landing pages in their system that convert to the finest.
What is Instapage Clickfunnel Vs Instapage
free trial, landing pages, landing page, business plan,
Lead Generation– Instapage is understood for having some truly amazing lead capture page structure capability. Their page builder is extremely flexible and has all readily available features required to provide a genuinely efficient lead generating page. There are a range of campaign-specific design templates that you can choose from.
Whether it's for getting downloads, signups, or sales, the Instapage lead generation tool will permit you to link the dots between ad campaigns and lead nurturing.
Instapage permits you to create different kinds of kinds, including multistep types and producing a kind with the Instapage Kind Home builder very easy.
Instapage Free Trial & Evaluation 3
Multistep forms are advised, as it breaks down what is potentially a long procedure for type filling into smaller sized procedures, thus making the procedure less tiresome and increasing conversions. This also makes gathering essential details simpler for potential customers.
In addition to this, Instapage has a variety of tools which make structure efficient landing pages a breeze.
Drag & Drop Home Builder — A genuinely drag and drop choice here. You can quickly include individual elements, exactly where you desire them.
Widgets — Instapage allows you to add lots of widgets like CTA buttons, Countdowns, and so on. You can likewise include Google Analytics, Facebook pixel, and Google Tag supervisor just by adding your ID.
All set Templates — Quite a a great deal of readymade templates are offered. You have design templates for all type of usages– whatever from list building, eBook's webinars, occasions, apps, click through CTAs, etc.
You can also easily import lading page design templates yourself which you can, later, customize for your own use.
Stock Images — Bigstock Images are readily available.
Landing pages that are incredibly basic tend to transform better than long ones that go on and on and babble. If you need to have a longer landing page since it helps you address objections, objections are concerns people have before they choose in or purchase from you, then that's fine, have a long landing page. You do not desire actually fluffy long landing pages that go on and on like I am right now with this point, since you're gon na lose people.
Reporting — Heatmaps, A/B testing, Google Tag Manager, and more.
google ads, post-click landing pages, landing page
Instablocks — Instapage permits you to conserve specific landing page sections to reuse throughout styles. They provide a fantastic way for clients to prevent producing duplicate material and focus more on developing more recent material, making the total process of marketing and advertising way faster and simpler.
AMP Support — Permits access to googles AMP protocol
Pay per click compatibility — Instapage enables you to include your landing pages to ad groups.
A/B Testing — Instapage allows you to test the effectiveness of your landing pages, therefore enabling you to utilize the very best variation for maximum conversions.
Heatmaps — A very helpful representation to test the effectiveness of your landing page layout. A heat-map will allow you to examine how far customers have actually scrolled on your page and where they dropped off.
These are a few of the function sets when effectively utilized, these can actually assist anyone in fact attain huge conversions.
Combination– There is a large range of combinations supported by Instapage and a lot are being added every other day.
Below are a few of the applications that Instapage integrates with:
Email marketing and marketing automation software (Hubspot, Mailchimp, AWeber, Consistent Contact, Campaign Screen, GetResponse, e-goi, mad mini, Marketo, InfusionSoft, Active Project, Autopilot).
CRM– incorporate your data with companies like (SalesForce, Zoho CRM, InfusionSoft, Active Campaign).
Webinar (GoToWebinar).
Analytics (Google Analytics, KISSmetrics, Mixpanel, Marketo).
Platforms (WordPress and Drupal).
Social media (Facebook).
Workflow (Zapier).
Furthermore, Instapage allows you to release to an endless variety of Domains. Other Landing page contractors will typically not permit that or will charge extra for the function.
Support– The Instapage assistance team is available 24/7. There is a chat and email assistance readily available at all times and there is a relatively comprehensive knowledge base that covers most concerns customers may have. Business clients likewise have access to telephonic assistance.
You'll get every feature you'll usually require and its ease of use is what is making it so important for your company compared to other CMS or page home builders, like WordPress and Squarespace.
Instapage "hidden" expenses + pricing information.
There is no long term free plan, however they let you sign up for an Instapage Free Trial for 2 week (no CC. required).
You'll still require to have a URL of your own. You can utilize Instapage's XXX.pagedemo.co subdomain, but it does not look extremely professional. Purchasing a domain isn't pricey, but remember the additional expense and time (costs about 24 hours to get a domain name).
Refund-period is only 24 hours! After that, your fee is non-refundable and your membership can just be stopped at the end of your membership period (depending on your regular monthly or annual agreement).
If you decide to opt for a yearly contract, you'll get a 23% discount. You do have to pay upfront with an annual subscription, so be prepared.
No covert costs, you will not pay for anything extra. It's a set charge.
Instapage is a strong Page Builder & lead generator Tool. It's easy to use, has a lots of functions and if used correctly can undoubtedly increase your conversions exponentially. We heartily recommend Instapage, although we would request you to take a look at its knowledge-base initially to optimize your efforts and make sure maximum output.
Click on this link for your Instapage Free Trial.
a "Special Visitor"?
A special visitor is any person who has checked out any of your landing pages. Distinct visitors are determined and tracked by an Instapage cookie and are counted as 1 special visitor (regardless of the variety of times they visit your page). Clickfunnel Vs Instapage


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By Cheryl G. (Poncer)
It is hard to believe that come the fall, PaperBackSwap will be 10 years old! What began as a way to swap books with other avid readers across the country has turned into so much more.
For me, it has become a lifeline, a lifeboat, a connection to friends near and far. I have had the pleasure to meet and make friends with folks across the US, friendships that will last a lifetime.
I first found PaperBackSwap in a tiny blurb in a Women's Day magazine while waiting for my husband in some doctor's office, there were too many doctors at the time to even remember which one.
I tore out the blurb (yes, selfish!) and stuck it in my pocket. It was deposited on my desk with the other scraps and items that my pockets always seem to collect. And there it sat for months and months. After he passed away, I found the scrap of paper and decided to investigate. Heaven knows I had too many books just sitting collecting dust. Some I had read upwards of 3 times, whiling away the hours of endless doctor visits and hospital stays.
In 2006 I signed up for an account. Being the suspicious person I was trained to be, I keep looking for the "catch". Never did find it, and soon enough my cynical side took a backseat. The members were so kind! And generous! And funny! And the friendships started within the very first few months of my being a member. Receiving books was great, forming friendships was life altering.
And that bring us to the cruise in October 2014 for Members and their family & friends.
We are sailing from Tampa, Florida on Norwegian Cruise Lines, on the Norwegian Sun.
We will embark on October 26 and return to Tampa on November 2, 2014.
Come join The PaperBackSwap Team and Tour Guides on a 7 day cruise to
- Roatan, Bay Islands, Honduras
- Belize City, Belize
- Costa Maya, Mexico
- Cozumel, Mexico
While we are at sea, your Tour Guides and Cruise Committee will be hosting multiple Wishlisted Book Swaps, a PBS Trivia Contest, a Scavenger Hunt, a Duct Tape Contest, a Q&A Session with members of the PBS Team, a Halloween Costume Contest, a Genre Dinner, Get-to-know-you Bingo, PBS Team Meet & Greet and a Cocktail Party. Our final stop will be Cozumel, Mexico, where lucky raffle winners will take a Jeep ride around the island with PBS founder Richard Pickering.
Inside Cabins are $535 per person. Oceanview Cabins range from $615 to $635 per person. Space is limited–the cabins are almost gone, and once they are all gone we cannot get additional cabins at these prices! Registration is open until June 30th or until cabins sell out. Don't let the ship leave without you. You can register for the cruise by filling out this form and our Travel Specialist will contact you. Details are available in the Discussion Forum topic.
Come with us, meet some new friends, get together with old friends, celebrate PaperBackSwap' 10th Anniversary with us on the sea!
"There are no strangers here; Only friends you haven't yet met." ~ William Butler Yeats
"The world is round so that friendship may encircle it." ~ Pierre Teilhard de Chardin
"Friends… they cherish one another's hopes. They are kind to one another's dreams." ~ Henry David Thoreau
Tags: Members, Milestones