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Category / ATL Marketing
What Is The Importance Of An Awareness Campaign That Is Aimed At The General Population?
The awareness campaign is important as it addresses a large audience. The campaign aims to leave a powerful impact on the people and help them cultivate some new behaviors. This will also…
February 21, 2021 -
Promoting your marketing campaign
To get the most value out of your marketing campaign, it is necessary to make it targeted towards and accessible to as many people as possible. Moreover, if you want most entries,…
February 21, 2021 -
How Important Is It To Have A Campaign Aimed At The General Population
Mass media campaigns have continued to be used as a device for advertising public health, and their effectiveness has been estimated and expressed in different writing. Some studies associated with triumphant campaigns…
February 11, 2021 -
Social Media Likes
Social Media- we are all part of it. As per statistics as of January 2020, around 3.6 billion people worldwide use social media, that's almost half of the world's population! The same…
February 8, 2021 -
Is ATL marketing a good option for start-ups?
ATL Marketing stands for Above The Line Marketing and generally refers to the kind of marketing that has an extensive and broad reach for example a national tv campaign where the audience…
January 2, 2021 -
Try out your own versions of some amazing fast-food specialties at home!
Are you a foodie and like experimenting with your food? Then you have landed at the right place. We will be telling you how you can make some of these easy recipes…
December 28, 2020 -
What is B2B and B2C marketing?
B2B or Business to Business marketing is when you promote your products or services to another business. For example, if you are a tire company and you are promoting it to a…
December 22, 2020 -
Sales promotion and how it works.
Sales promotion is one of the central components of the promotional blend. To build dependable, long lasting associations with clients, you need to discover the sorts of deals that work with your…
December 22, 2020 -
A guide to proper affiliate marketing!
Affiliate marketing is a rather straightforward concept, it is the process or the strategy to go about it that can be quite complex. If you're looking to market your products through affiliate…
December 18, 2020
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Plastic cards are more than just simple plastic print products in credit card format. When we print your plastic cards, they are transformed into chic membership cards, secure access cards or attractive customer cards. You are completely free to choose your motifs and imprints yourself. Whether in corporate design or with simple plain lettering, with print24k.net you rely on one of the biggest online printers in Europe to realise the plastic cards professionally and in top-quality at low cost. It makes no difference whether you start with 25 or need 50,000 cards – thanks to our state-of-the-art printing technology, we can reliably and rapidly realise any order size.
We all know them, the moments just before a birthday, before Christmas or another holiday: we haven't got a present. Since gift vouchers have conquered the market, giving presents has become easier for many people. A specific amount of money is loaded on the high-quality designed plastic card for the recipient to redeem later on whatever he/she likes most in the relevant shop. With print24k.net, you can have a gift or shopping vouchers printed for your business. You can choose between simple plastic cards or more elaborate print products with a magnetic strip or smart card, which can be loaded with credit over and over again.
All plastic cards, whether equipped with chip, magnetic strip or RFID technology, are made at print24k.net from top-quality plastic. This is then varnished or laminated to give the cards the sturdiness they need for daily use.
The format of the cards is always 85.6 x 54 mm. This way, these cards can be comfortably kept in credit card cases and provided with standard covers.
The simplest version with no extra features are the plastic cards. They are available in varnished plastic and laminated plastic in glossy or matt. Depending on the material you can then choose one of the following extras: LoCo magnetic strip, signature field, round or slotted hole. The matt and glossy cards can additionally be applied with various refinements.
With the chips cards you have the choice between positioning the chip on the left or the right side. As an extra, you can add the following options: LoCo magnetic strip, signature field, round or slotted hole. If you need chip cards, we have two different versions available:
There are two versions of magnetic strips available at print24k.net. Our swipe cards are equipped with a HiCo (high coercivity) magnetic strip by default, enabling your plastic card to store data for a longer time because the magnetic strip is more robust. Identity or security cards are often provided with this kind of magnetic strip. LoCo (low coercivity) magnetic strips, in contrast, posses a lower flux density and can be added to our chip, plastic and RFID cards as an extra. These LoCo magnetic strips are used for data that changes frequently, for example, for daily access changes to hotel rooms.
A special variant of the plastic cards printable here are RFID cards. The use of "radio frequency identification" allows, say, the users of these cards to provide contactless identification. The data on the NXP MIFARE® Classic 1K chip can actually be read by means of radio waves at a frequency of 13.56 MHz up to a distance of 10 cm. This form of plastic cards is in common use today for student identity cards, library passes, time recording systems and access controls. The RFID-cards are made from glossy laminated plastic by default and you can add the LoCo magnetic strip or a signature field as an extra.
If you want magnetic or plastic cards for your membership cards or other products printed at print24k.net, you can choose between various refinement and security options. This makes them better protected against forgery. We use laser technology to insert the hologram into the card. You have two designs to choose from (VIP and a globe). Other available refinement options are hot embossing, embossing on the left/right side as well as a thermal transfer print on the left/right side.
Here is an overview of the available refinement options:
We print all plastic cards on both sides in landscape format using the 4/4 CMYK printing process. In this way, you have the greatest possible flexibility in the choice of motif and text. With the four basic colours, all possible colours can be produced directly during printing.
At the same time, the high quality of our colours also ensures that you can print photos or high-resolution graphics true to detail and with a high colour depth. In the plastic card version, you also have the option of combining CMYK with the special silver colour.
We can recommend a yo-yo in a small version or oval in black or white-transparent as a practical accessory. As protection for the cards, you can also order a high-quality identity-card holder in portrait format or a soft PVC cover in portrait or landscape format. If your plastic cards are to be used at trade fairs or in companies for employee identification, then we offer practical metal clips with a plastic or textile buckle, which you can choose in the configuration menu.
On request, we can also equip your plastic cards with other functions. We can equip the chip, plastic and RFID cards with a magnetic strip. A field for the user's signature can be added to any version of cards. Additionally, we can include a round or slotted hole with any card, except the RFID-card, for attaching the card to a key ring. The most features are available when printing simple plastic cards.

subset: commoncrawl


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Bespoke Vintage UK
Shipping: £8.95 UK Mainland
A very attractive vintage chintz Jardinière plant pot - punch bowl, of good size. No chip or cracks, slight crazing.
This is very usable and would look in place with today's fashion.
In lovely condition, a great addition to any home or gift for someone special.
Diameter; 10" (25cm)
Height; 6" (15cm)
If you are outside the UK Mainland and wish to make a purchase, please get in touch for details.
Additional information:
As we deal in original antique and vintage items, it is worth noting that this object has been pre-loved and will have signs of use, wear and tear. Wherever possible we will always list the condition of the item, but it should be noted that these are not new. We feel this adds patina and creates the beauty of the object. If you have any doubts or questions, PLEASE contact us BEFORE purchasing the item. We also reserve the right to remove or refuse any sale, at which time a full refund would be issued. Please see our terms and conditions of sale.


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New Lecture "Offroad-robotics" starts the 25.10.
The first lecture on "off-road robotics" by Dr.-Ing. Patrick Wolf starts the 25th of October at 08:15 - 09:45 a.m. in room 48-210.
Christian Heupel won the price for Stiftung Pfalzmetall 2023
Prof. Karsten Berns celebrates 20 year anniversay of RRLab
Unimog Demonstration
Sat has published a new video about the Unimog demonstration of the chair.
5. Konsortialtreffen NaLamKI in Erfurt
Lectures SS 2023
In the upcoming summer semester, the lectures "Autonomous Mobile Robots" and "Foundations of Embedded Systems" will be given by RRLab. Furthermore,…
RPTU: Hannover Messe 2023
An article on the road to smart production
DW: Robot as rescuer after earthquake
An interview about LUGV/SUGV robots as earthquake rescuers.
1730Sat1Live: Fast menschlich - Roboter Emah aus Kaiserslautern
The tv channel Sat has published a new video about the new humanoid robot from the robotics chair.
Robotics Research Lab
University of Kaiserslautern-Landau
Department of Computer Science
Robotics Research Lab
Prof. Dr. Karsten Berns
Building 48, Room 358
D-67663 Kaiserslautern
Phone: 0631 / 205 - 2689
Fax: 0631 / 205 - 2640
Email: email@example.com
Postal Address
University of Kaiserslautern-Landau
Department of Computer Science
Robotics Research Lab
Prof. Dr. Karsten Berns
P.O. Box 3049
D-67653 Kaiserslautern


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The largest mosque in Central Asia is the Astana cathedral mosque "Khazret Sultan" i.e. "Holy Sultan". The name was suggested by Nursultan Nazarbayev himself. As you know, "Khazret Sultan" is one of the epithets of the Sufi sheikh Khoja Ahmed Yasawi, the author of "Divan-i Hikmet" (XII century), who was a poet and philosopher known throughout Central Asia.
The mosque is located on the right bank of the river Esil in the north-eastern part of the city-planning ensemble, which includes the Palace of Peace and Conciliation, the Palace of Independence, the Palace of Creativity and the Museum of Archeology and Ethnography with the "Kazakh Eli" monument, in one of the priority urban areas of the capital. All personifies spiritual wealth, greatness of history and unity of the people of Kazakhstan.
On June 29, 2009, at the solemn ceremony of laying a commemorative capsule on the site of the construction of the cathedral mosque, Nazarbayev stressed that it would become one of the most beautiful architectural structures of the capital.
"The mosque is not built on public money, but on donations and sponsorship funds," he continued. In this connection, it is hoped that the Kazakh people will take an active part in this endeavor.
Before the start of the capsule laying, the chairman of the Spiritual Board of Muslims of Kazakhstan, the Supreme Mufti of the RK Absattar Kazhi Derbisali, conducted the ritual of "Bata", according to Muslim custom, a sheep was sacrificed.
As noted, the Supreme Mufti of the RK, it will be the 4th because in three mosques everyone can not fit, especially during the Muslim holidays.
During the construction of the mosque, 1,000 to 1,500 people worked.
It should be noted that during construction, natural construction materials from Kazakhstani fields were used, and those materials that were not enough were imported from abroad. They strictly observed the condition - to deliver only from the same quarry. For this, the customer and the general contractor of the construction were responsible. All this was necessary in order for the walls and coatings to be of a monochromatic color.
The construction was completed in 2012, and the mosque "Khazret Sultan" was presented on July 6, as it was later noted that it became a unique object of Astana.
In the structure they adhered to the classical Islamic style, and of course, they used traditional Kazakh ornaments. The mosque accommodates 5,000 worshipers, and on holidays - up to 10,000.
More than 11 hectares is the area of the entire mosque, and the area of the buildings is 17,700 square meters. Its dome, the height of which is 51 meters and the diameter of the base is 28.1 meters, is the largest in the country. Also it includes 8 small domes with diameters of 10.45 and 7.6 meters, and peaks of 33.46 and 25.25 meters.
At the corners are located four 77-meter high minarets. The temple is crowned by an 80-meter spire with a crescent directed strictly towards Mecca.
The wedding hall, dining room, ablution rooms of men and women with wardrobes are on the 1st floor. Men's prayer hall for four thousand people and a large lobby with a dressing room are on the 2nd floor. On the third floor there is a women's prayer hall for a thousand people, an office of the Imam and additional rooms of the Naib Imams. Also in the mosque there are rooms for ablution and ritual of the wedding, halls for reading the Koran and classes for educational groups.
Massive white columns, due to which the sense of monumentality of this structure is strengthened, hold the central hall. The decoration is a unique chandelier (2 tons). It was brought from Turkey.
Translated by Raushan MAKHMETZHANOVA


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Jiangsu Shinuo Lighting Co., Ltd., a high-tech enterprise of Jiangsu Province, established in 1998, has been specialized in R&D and manufacturing green & energy-efficient lighting products for more than 20 years. Now our major lighting product series are LED Indoor and Outdoor Lighting.
With professional technicians and well-equipped facilities, we provide not only quality products but also good services.
We have Programmable Temp.& Humi. Chamber, EMC laboratory, etc. in our factory, which enable us to do all the necessary strict tests for every new product. Customization is available.
We acquired CCC, CE, RoHS, CQC, ISO9001, ISO14001 and ISO45001 certificates. Our products are mainly exported to European market.


text: George Carlin once said "every pet is a tiny tragedy waiting to happen." True…unless perhaps you are Barbra Streisand. The singing icon known for her long career and even longer pockets, shed some light on her latest pet rearing endeavor in this weeks Variety. Back in 2017, Streisand's beloved Coton de Tulear, Samantha, passed away. Before the pet's death, Streisand took DNA samples of the dog and had her cloned, which is apparently a thing you can do when you are THE Funny Girl. The cloning resulted in two dogs, Miss Violet and Miss Scarlett, who are obviously physically identical to Samantha, but according to Streisand, do not have the same personalities. Keeping it in the family, Streisand has a third dog, Miss Fanny, who is actually a distant cousin to the original Samantha. With all of this cloning talk, you know there is a hamster in a shoebox in some suburban backyard just hoping for a second chance at life. Take a look at Babs and her pooches below, and check out this weeks Variety!


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How to start an idea is the focus of my post today. I am writing about this for obvious reasons. I have received lots of emails and answered lots of questions from people who have come across our various materials on how to start a big idea they have conceived.
I hope this post will go a long way to help you. In case, you want a full guide I wrote so many years to address this problem, please subscribe to our newsletter and you will be able to receive an email that contains the download link.
Related: If you want my online course on this topic, please click here
How to start an idea: Ideas are seeds
Ideas are seeds that must be planted
I am sure you are familiar with those tiny things called seeds even if you have not planted any before.
You see, those tiny things are very powerful. Though they look tiny. If they are planted in the soil and well nurtured they can become so big and plenty that a nation can be fed from them!
Now the keyword from my immediate statement above is "If they are planted in the soil and well nurtured."
How start an idea: Your big idea should start as a tiny seed
Your business idea when you received it is just like those tiny, powerful resources called seeds. It is a seed and, if is planted, it is able to grow and expand considerably beyond your wildest imagination and will be useful to bring up other business ideas and resources.
It is simple. If you truly get a hang on the fact that every business idea you receive at any time, no matter how big it is at the early stage, is a seed, then you have been able to win the first battle over the wrong and limiting mentality that make people abandon business ideas because of 'n money excuses'.
You should know that the gigantic picture of the idea you saw at the early stage does not necessarily mean you have to start from the level you saw in your mind when you conceived the idea.
It is a seed at this stage and it must start just exactly as seeds start their journey to germination and fruitfulness.
Jesus showed the way to start a big idea
Jesus was clearly pointing this fact out when He told His listeners that,
"Unless a grain of wheat falls into the ground and dies, it remains alone, but if it dies, it produces much grain." (John 12:24).
He was telling them that though He had come to carry out the big task of liberating men from sin and the devil and to qualify them as children to His Father but He would not be able to achieve that except He is sown as a seed into the ground!
Your big idea is achievable with God and if you see it as a seed you have to plant and nurture.
Join the Visionary Business Project train
You can join the Visionary Business Project train where you can learn Christian principles that can help start a business and run it the way God intends it.
Visionary Business Project: Raising God-fearing and problem-solving business owners and workplace professionals. Whatsapp/Call/SMS: 08034300979, 08185021024, 08124964351.


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Bald eagles, George Washington, Stephen Colbert, hot dogs, what more is there to love about America? Tomorrow marks one of the greatest holidays our nation celebrates. A day of honoring and celebrating the thirteen colonies gaining their independence from England back in 1776. We celebrate by doing things Americans do better than all other people in the world. There will be cookouts, fireworks, beer drinking, fishing, boating, music, and you better believe short sleeve button down shirts will be worn.
I love the 4th of July; in fact I will go out on a limb and say it's my favorite holiday. Sure the holidays in December are nice and all, families get together and give each other gifts. However, you're trapped inside for the weather is frigid. Also, what good is drinking a cold beer with the family when its 3 degrees outside? Tomorrow the cookouts across the nation will feature beef on top of beef, which will then be wrapped in bacon. Pasta salads will be covered in mayonnaise while beers are consumed by the case. July 4th gives everyone a chance to get the day off from work and enjoy some summer fun.
I for one cannot think of a more fun thing to celebrate. My mind has been ethnocentrically programmed to love America more than anything else. I get to celebrate how great we all are and the rich history our country holds. Think of the activities we have the privilege of doing just because we're American. We get to read the national addition of USA today. We drive inappropriately loud and obnoxious trucks. We have the option of shooting our own dinner entre, and if we fail, do not fret for we can buy an entire meal at McDonalds for $1.
I hope you all get out of the house and do the most American thing you can think of. Or at least take a moment to appreciate what our ancestors did to give us a reason to celebrate our freedom. So post up on a lawn chair and relax, drink a beer and gaze upon the fireworks, you're an American and you're great.


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How has your journey been?
Ever since childhood, I just wanted to be an actor. And now, it's like my dream come true.I was born in Ambala. Vivek Shauq who acted in 'Gadar' was like my cousin. I met him through Satsang when I was only 7 years old. My father Hansraj Wadhwa used to work in the entertainment industry in direction. He did a few films but that's that. I acted in one of his films called 'Heroine Ek Raat Ki' when I was 6-7 years old.
How did you end up taking acting as a profession?
I joined theatre, did intercollegiate competitions and won many awards. I won the Best Actor award five times during my University days. I won the Balraj Sahni Trophy for Outstanding Performance. When you get acknowledgment and appreciation you believe that you're on the right path.
Tell us about your maiden acting opportunity
I am Ramesh Talwar's student and my first big break was with his play, with Mrs Jaya Bachchan, I played her son. I had acted in his serial 'Khatta Meetha' which was like 'Dekh Bhai Dekh'. I was skeptical about doing serials at that time and wanted to concentrate on films. Then I got a good film called 'Rahul', directed by Prakash Jha and produced by Mukta Arts. I played the villain in it. Since the movie didn't work, I went back to doing serials.
My posters for the show Kohinoor were put up all over Mumbai. It was that huge. Because of Kohinoor, I got Ram Gopal Varma's film 'Shabri'. The film began in 2005 and was released in 2011, by that time I was doing 'Chanakya'. I didn't even recognize myself in Shabri because I was bald for Chanakya. Chanakya was a life-changer for me.
Share your experience of working with Jaya Bachchan
We were there in the US for two months for that play. I call her Maa. I played her son in two plays, Maa Retire Hoti Hai and Dr. Mukta. She meets you first and if she thinks that she can work with you only then you're in the play. Her observation skill is very sharp. She will tell you directly that she won't be able to work with you. I was like her son and bodyguard. I used to be like her shield. My working experience with ji was wonderful.


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Boxing legend Manny Pacquiao has goals for life after his career is over. He wants to own an NBA team.
The 40-year-old Senator of the Philippines loves basketball and is the founder of the Maharlika Pilipinas Basketball League (MPBL).
The only question is when would he retire from boxing? Pacquiao has shown no signs of slowing down and is taking on the undefeated Keith Thurman on Saturday night.
Flip the Page to Listen to Pacquiao discuss owning an NBA team:


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LA Times Crossword answers Friday 21 June 2019
Thank you for choosing our answers site. This is the latest update from the world of the Los Angeles Times.
In this update you will find unique crosswords to solve. It is likely that some of the crosswords are extremely difficult for you to solve and you will need our help.
That's why we decided to share all the LA Times Friday 21 June 2019 crossword answers with you. Exercise your brain and work your way through the bright crosswords released daily!
After clicking on one of the crosswords we have shared with you, you will be redirected to the corresponding level of the game.
If you are stuck or have something wrong, please contact us and we will be happy to help you.
Los Angeles Times 21 June 2019 crossword
Complete List of Clues/Answers
1 It's sold in bars: SOAP
9 Live: DWELL
14 Willing follower? ABLE
15 Forked over: PAID
16 Certain Ivy Leaguer: YALIE
17 That's a wrap: GIFTBOXCOVERING
20 Make more flavorful: SEASON
22 Fascinated by: INTO
27 That's a wrap: TERRYCLOTHROBE
35 Bushy-tailed canines: FOXES
36 Eat-on-the-street places: CAFES
37 What an amateur may turn: PRO
39 Goes from site to site: SURFS
42 Start a court contest: SERVE
43 Radiates: EMITS
44 That's a wrap: ROLLEDSANDWICH
48 Italian god: DIO
49 Made it up: LIED
55 Evades: SKIRTS
60 "That's a wrap!": ENDOFAFILMSHOOT
63 Frequent genre for composer John Williams: SCIFI
64 Bay, play or gray follower: AREA
65 Kurylenko of "Quantum of Solace": OLGA
66 Identity __: THEFT
67 Provoke: ROIL
1 Loses elasticity: SAGS
2 "Hamilton" award: OBIE
3 Opposite of Zulu? ALFA
5 Like angel food cake: SPONGY
6 Strain: TAX
7 Bothersome bugs: LICE
9 Salon supply: DYE
10 Not as trusting: WARIER
13 Danish brick: LEGO
18 Male razorbacks: BOARS
19 Iconic Chevys: VETTES
24 Goes down, so to speak: OCCURS
26 Reactions to missing things: EHS
27 2013 role for Johnny Depp: TONTO
28 Get all A's: EXCEL
29 Common hummingbird feeder color: RED
30 Cocoon dweller: LARVA
31 Cause resentment: OFFEND
32 __ disc: eye part: OPTIC
33 Stock: BROTH
34 Quite a stretch: EONS
35 Common par: FOUR
39 Resort near Flagstaff: SEDONA
40 Former Radiohead label: EMI
43 "Star Wars" critters: EWOKS
45 Started: LEDOFF
46 Dreary: DISMAL
50 Rainfall measure: INCH
51 Singer Brickell: EDIE
53 Miles away: AFAR
56 2007 Applebee's acquirer: IHOP
57 Part: ROLE
58 Costume made from a sheet: TOGA
62 Honolulu Airport wreath: LEI
Go back to crossword list


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In many flowering plants, petals initiate in alternate positions from first whorl sepals, suggesting possible signaling between sepal boundaries and petal initiation sites. PETAL LOSS (PTL) and RABBIT EARS (RBE) regulate petal initiation in Arabidopsis thaliana and their transcripts are expressed in sepal boundary and petal initiation sites, respectively, suggesting that PTL acts in a non-cell-autonomous manner. Here, we determined that cells expressing PTL and RBE fusion proteins did not overlap but were adjacent, confirming the non-cell-autonomous function of PTL. Genetic ablation of intersepal cells by expressing the diphtheria toxin-A chain gene driven by the PTL promoter resulted in flowers lacking petals, suggesting these cells are required for petal initiation. Transcriptome analysis combined with a PTL induction system revealed 42 genes that were upregulated under PTL activation, including UNUSUAL FLORAL ORGANS (UFO), which likely plays an important role in petal initiation. These findings suggest a molecular mechanism in which PTL indirectly regulates petal initiation and UFO mediates positional signaling between the sepal boundary and petal initiation sites.
Flower morphology is important for attracting pollinators, such as flying insects or birds. The arrangement of floral organs in floral buds is roughly classified into two patterns: the spiral pattern, observed in several clades, including basal angiosperms (Endress, 2001; Endress and Doyle, 2007); and the whorled (or concentric) pattern seen in many other flowering plants. Flowers generally consist of four types of floral organ: sepals or outer tepals; petals or inner tepals; stamens; and carpels. In whorled flowers, different organ identities in each whorl of the flower are specified by distinct complexes of floral homeotic proteins. This regulation is explained by the floral ABC model or the more advanced ABCE model, which both describe the genetic mechanism for the establishment of floral identity in most flowering plants (Theissen et al., 2016).
Floral organs generally arise in a more or less equally spaced pattern within the whorl. In Arabidopsis thaliana, two medial sepals arise on the adaxial and abaxial sides of the inflorescence meristem, followed by two sepals at the lateral positions at floral stage 3 (Smyth et al., 1990). The second whorl contains petals, which usually occur in alternate positions to the sepals (or outer tepals). This suggests that positional information for the intersepal region is transmitted to the inner petal founder cells to fix the organ positions within the second whorl. However, the molecular mechanism for this positional signaling remains unknown.
Genetic approaches have identified regulators involved in the early development of second whorl organs in Arabidopsis. The petal loss (ptl) mutant has defects in sepal separation and petal initiation (Griffith et al., 1999). PTL encodes a GT2-clade trihelix transcription activator and is expressed in intersepal cells. These cells do not overlap with cells expressing the auxin-responsive DR5 reporter gene, suggesting that PTL regulates petal development indirectly (Brewer et al., 2004; Lampugnani et al., 2012). RABBIT EARS (RBE) encodes a C2H2-type zinc finger protein and its mutation results in the deformation or elimination of petals (Takeda et al., 2004). RBE is expressed in petal primordia cells, in which it negatively regulates the transcription of AGAMOUS, MIR164, TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR 4 (TCP4), TCP5 and PUB25 to facilitate the early development of petal primordia (Huang and Irish, 2015; Huang et al., 2012; Krizek et al., 2006; Li et al., 2016, 2021). When the ptl or rbe mutant was combined with the homeotic mutant apetala3 (ap3), the second and third whorl organs of which are replaced by sepals and carpels, respectively, the double mutants showed a reduced number of sepalloid second whorl organs (Griffith et al., 1999; Takeda et al., 2004), suggesting that PTL and RBE are involved in the development of second whorl organs independently of organ identity.
Several pieces of evidence suggest that PTL and RBE regulate petal initiation in the same pathway: first, both mutants show similar petal-loss phenotypes; second, the ptl rbe double mutant resembles the ptl single mutant (Lampugnani et al., 2013); and third, RBE expression is absent in the petal primordia of the ptl mutant (Takeda et al., 2004). These findings suggest that positional signal transduction occurs between the sepal boundary, where PTL is expressed, and the petal primordium, where RBE is expressed.
Here, we uncovered a molecular link between the sepal boundary and petal initiation site and the role of this link in petal initiation. Using fluorescent fusion proteins, we examined the spatiotemporal expression patterns of PTL and RBE in floral buds. PTL was detected in sepal boundaries and RBE was detected in petal initiation sites; the fluorescent signals were adjacent but not overlapping, confirming the notion that PTL regulates petal primordium initiation indirectly. We used the glucocorticoid induction system to enable temporal activation of PTL and determined that several genes were activated by PTL, including UNUSUAL FLORAL ORGANS (UFO). The ptl ufo double mutant resembled the ptl single mutant, suggesting that UFO acts downstream of PTL. Our findings shed light on the signal transduction of positional information between the sepal boundary and petal initiation sites, which is required for early petal development.
PTL regulates petal initiation in a non-cell-autonomous manner
Previous studies showed that PTL is expressed in sepal boundary cells, whereas the ptl mutant either lacks, or has deformed, petals, suggesting that PTL regulates petal initiation in a non-cell-autonomous manner (Brewer et al., 2004; Griffith et al., 1999). To investigate whether PTL moves from intersepal cells to petal initiation sites, we generated a translational fusion of PTL and CFP expressed by the PTL own promoter and terminator (PTLg:CFP; Fig. S1). The construct restored petal development in ptl-1 and the fluorescent signal remained at intersepal cells (Fig. 1A,E,I), suggesting that the fusion protein was functional and that PTL functions in a cell-autonomous manner at the sepal boundary.
To elucidate whether the PTL-expressing region contains cells that give rise to petals, we generated GFP:RBEg PTLg:CFP plants. RBE is transcribed and translated in petal primordium cells and is essential for petal primordium initiation (Fig. 1B; Takeda et al., 2014). The expression domains of PTL and RBE did not overlap but were adjacent (Fig. 1C,D; Movies 1 and 2), indicating that the domains of the intersepal and petal initiation sites were neighboring but did not overlap.
To confirm the PTL expression at the sepal boundary, we crossed PTLg:CFP plants with a line expressing fluorescently labeled proteins in boundary domains. CUP-SHAPED COTYLEDON1 (CUC1) and CUC2 are expressed in the organ boundaries in the aerial organs of plants and regulate boundary identity (Aida et al., 1997; Ishida et al., 2000; Takada et al., 2001). The translational fusion of CUC1 and GFP in CUC1g:GFP plants was expressed in the boundary of the sepal and floral meristems (Fig. 1F), whereas the CUC2-GFP fusion protein in CUC2g:GFP plants was expressed in a broader region compared with CUC1 (Fig. 1J). Plants carrying both PTLg:CFP and CUC1g:GFP contained a few cells in which both signals were detected at the sepal boundary (Fig. 1G,H; Movie 3). By contrast, PTLg:CFP CUC2g:GFP plants contained more cells expressing both PTL and CUC2 at the sepal boundary (Fig. 1K,L; Movie 4). These data support the notion that PTL is transcribed and translated in the sepal boundary and, thus, regulates petal primordium development in a non-cell-autonomous manner.
PTL-expressing cells are required for petal initiation and development
To examine whether sepal boundary cells are required for petal initiation, we genetically ablated these cells using diphtheria toxin A chain (DT-A) (Bellen et al., 1992). DT ribosylates the EF2 translation initiation factor and inhibits protein synthesis, causing cell death. DT-A is a component of DT that kills cells in a cell-autonomous manner and has been used for tissue-specific genetic ablation in plants (Day et al., 1995; Nilsson et al., 1998; Takeda et al., 2004; Tsugeki and Fedoroff, 1999). We generated PTLg:DT-A plants in the wild-type (Colombia-0; Col-0) background. Among the 24 lines isolated by screening in the T1 generation, nine lines showed a small stature, with narrow, dark-green leaves, and subsequently developed a few flowers (Fig. 2A). Narrow leaves suggest cell death in the edge region, where PTL is expressed (Brewer et al., 2004). Eleven other lines were small, with small, green leaves and set of flowers with reduced numbers of petals and stamens (Fig. 2B; Table S2). The other two lines showed normal vegetative growth and set seeds, and their offspring generated flowers with fused sepals (Fig. 2C) and fewer petals compared with Col-0 (Fig. 2D), resembling the phenotypes of the ptl mutant. Some petals exhibited serration at their proximal regions (Fig. 2E). These results indicate that genetic ablation of PTL-expressing cells affects sepal separation, petal initiation and petal morphology, confirming the notion that the sepal boundary is required for petal initiation.
Downstream genes regulated by PTL
To identify genes involved in positional signal transduction, we examined genes regulated by PTL. We developed a chemical induction system of PTL using the rat glucocorticoid receptor (GR; Aoyama and Chua, 1997). A translational fusion of PTL and GR was expressed under the control of the PTL promoter and terminator (PTLg:GR) in the ptl-1 mutant background (Fig. S1). First, we applied mock or DEX solution onto each inflorescence once daily. The mock treatment did not restore petal formation in the mutant (Fig. 3A), whereas DEX-treated inflorescences developed petals after 7-10 days (Fig. 3B), indicating that the PTL-GR fusion protein was functional. Next, to determine whether the temporary action of PTL is sufficient for petal initiation, we applied mock or DEX treatment to inflorescences only once, finding that petals developed after 10 days in DEX-treated plants but not in mock-treated plants (Fig. 3C,D). According to the floral stages defined by Smyth et al. (1990), an open flower at stage 13 was at stage 5 (bud) 10 days earlier, when petal and stamen primordia arose. Therefore, when flowers at the petal initiation stage (i.e. around stage 5) were induced to express PTL by DEX treatment, they successfully initiated petal primordia. This further suggests that, once PTL is activated, subsequent stages of petal development progress to the final steps in morphogenesis. Our data highlight the importance of PTL as a master regulator of petal initiation and development.
Using this induction system combined with RNA-sequencing analysis, we identified genes that function downstream of PTL. We identified 42 upregulated and seven downregulated genes in the inflorescences 3 h after DEX treatment compared with mock control plants (Fig. 3E; Table 1). Among the upregulated genes, we focused on the F-box gene UNUSUAL FLORAL ORGANS (UFO)/At1g30950; the upregulation of this gene was confirmed by RT-qPCR (Fig. 3F). These results suggest that UFO plays a major role in petal development under PTL regulation. We also examined UFO expression in PTLg:GR plants by mRNA in situ hybridization. UFO is expressed in the center of the floral primordium at stage 2, in the cup-shaped domain during stage 3 and in four clusters at the base or abaxial side of the petal primordium at stage 4 (Durfee et al., 2003; Ingram et al., 1995; Laufs et al., 2003; Lee et al., 1997; Samach et al., 1999). In mock-treated plants, UFO was expressed in the cup-shaped domain at stage 3 (Fig. 4A) but was absent from four clusters at later stages (Fig. 4B). By contrast, in DEX-treated inflorescences, UFO was expressed in four clusters at stages 4 and 5 (Fig. 4C,D), supporting the notion that PTL regulates UFO expression specifically in these four clusters.
To confirm the genetic relationship between PTL and UFO, we generated ptl ufo double mutants. We used a weak ufo allele, ufo-6, to examine the effects of ptl on petal development (Lee et al., 1997; Levin and Meyerowitz, 1995). Flowers of the ptl-1 ufo-6 double mutant resembled those of the ptl-1 single mutant (Fig. 4E-H), which is consistent with our hypothesis that UFO acts downstream of PTL.
At1g11620, encoding an F-box protein in a family distinct from UFO (Kuroda et al., 2002), was also highly upregulated in DEX-treated PTLg:GR plants (Table 1; Fig. 3G). We examined the T-DNA insertion mutant for this gene (SALK_068307) but did not detect any differences from the wild type in whole plants, including flowers. Therefore, whether this gene is involved in petal initiation remains unknown.
Interestingly, TDF1/At3g28470 was also upregulated in DEX-treated PTLg:GR plants (Table 1; Fig. 3H). TDF1 encodes an R2R3 MYB transcription factor that affects late tapetal function and subsequent pollen development (Zhu et al., 2008). TDF1 expression is first detected in anthers at anther stage 5, corresponding to flower stage 9 (Sanders et al., 1999; Smyth et al., 1990; Zhu et al., 2008). PTL is expressed in lateral regions of the stamen primordium at floral stages 7-9 (Brewer et al., 2004). Although their expression patterns need to be examined in detail, it is possible that PTL activates TDF1 expression in the stamen primordium.
PTL is suggested to regulate petal initiation in a non-cell-autonomous manner and we confirmed this here by protein localization at the sepal boundary. Using a DEX induction system and transcriptome analysis, we identified 42 upregulated genes in DEX-treated PTLg:GR plants versus controls. Among these, we focused on the role of UFO in floral organ development. UFO was expressed in four clusters upon PTL induction (Fig. 4). The ptl-1 ufo-6 double mutant resembled the ptl-1 single mutant (Fig. 4), and ufo is epistatic to rbe-3 in the development of the second whorl organs (Krizek et al., 2006), suggesting that UFO mediates positional signaling between PTL and RBE.
UFO, an F-box protein belonging to the SCF ubiquitin complex, regulates multiple processes during floral organ development (Ingram et al., 1995; Laufs et al., 2003; Levin and Meyerowitz, 1995; Wilkinson and Haughn, 1995). One of the major roles of UFO is to activate class B homeotic genes, especially APETALA3 (AP3), with other components of the SCF complex (Lee et al., 1997; Ni et al., 2004; Samach et al., 1999; Wang et al., 2003; Wuest et al., 2012; Zhao et al., 2001). The activation of AP3 is mediated by the direct interaction of UFO with LFY, which directly binds to the AP3 promoter for transcriptional activation (Chae et al., 2008). AP3 binds to the terminator region of RBE and regulates its transcription (Wuest et al., 2012), suggesting that UFO is involved in activating RBE via AP3 activity. However, because AP3 is expressed in a whorled pattern, whereas RBE expression is restricted to the petal primordia (Takeda et al., 2004), another mechanism must exist for the positioning of gene expression to the organ primordia in the second whorl.
The other function of UFO is to regulate petal initiation: some ufo alleles result in flowers lacking petals (Durfee et al., 2003). UFO is expressed in floral meristems at stage 1, in three inner whorls at stage 2, in a cone-shaped region at stage 3, in four clusters lying on the abaxial side of the petal primordia at stage 4 and in petal primordia from stages 5 to 6 (Durfee et al., 2003; Ingram et al., 1995; Laufs et al., 2003). Therefore, UFO is likely involved in the position-dependent initiation of petal primordia at stage 4.
We propose a genetic mechanism for petal initiation in the second whorl involving two pathways: a pathway for the determination of organ identity in a whorled pattern; and a pathway for organ positioning (Fig. 5; Fig. S2; Table S3). LFY and UFO activate class B genes, which establish the petal identity together with class A genes in the second whorl. Simultaneously, PTL is activated in the sepal boundary region, likely by CUC1, given that transcriptome analysis showed that PTL is upregulated in 35S:CUC1 plants (Takeda et al., 2011). PTL expressed in the sepal boundary then activates UFO, and UFO activates RBE in the petal primordia. We examined UFO expression in PTLg:GR plants 3 h after treatment with cycloheximide and DEX treatment but did not detect its expression. Moreover, it appears, although not definitely so, that four clusters in which UFO is expressed at stage 4 include intersepal cells; thus, whether UFO is a direct transcriptional target of PTL remains unknown.
There may be crosstalk between these two pathways, because AP3 directly activates UFO and RBE (Wuest et al., 2012), and RBE suppresses class B genes (Takeda et al., 2014). The latter negative feedback regulation might enable temporal expression of RBE during petal initiation. Together, these signals might be integrated and lead to the initiation of petals at alternate positions from sepals in the second whorl at floral stage 5. Given that UFO is a component of the SCF complex, we propose that a protein degradation process occurs during petal initiation. Indeed, several F-box genes were upregulated in DEX-treated PTLg:GR plants (Table 1), suggesting that proteolysis is an important process for position-dependent petal initiation.
PTL is thought to promote auxin accumulation at petal initiation sites, and auxin-related factors, such as AUX1, PID and PIN, act downstream of PTL (Lampugnani et al., 2013). Expression of AUX1 at intersepal cells driven by the control of the PTL promoter restored petal development in the ptl mutant, suggesting that auxin biosynthesis at intersepal cells is required for petal initiation. However, in this study, we did not identify any upregulated auxin-related genes under PTL induction (Table 1). IAA29, an auxin-responsive gene, was downregulated in DEX-treated PTLg:GR plants based on transcriptome data, but RT-qPCR analysis showed that this difference was not significant (Table 1; Fig. S3). Therefore, although the PTL-dependent auxin pathway is crucial for petal initiation, we suggest that this type of regulation occurs later than the early response of transcriptional regulation by PTL.
The differentially expressed genes in PTL-induced plants included several genes encoding transcription factors, secondary metabolic enzymes and transporters (Table 1), but we did not identify genes that were reported to be involved in petal development. Given that PTL is expressed not only in the sepal boundary during early stages of flower development, but also in the lateral domains of sepals and petals (Brewer et al., 2004), the differentially expressed genes may include genes involved in the development of the lateral regions of perianth organs, such as TDF1 (involved in stamen development), as shown above.
In conclusion, we suggest that the F-box protein UFO mediates the signaling of positional information between the sepal boundary (PTL) and petal initiation sites (RBE). This idea might explain how PTL regulates petal initiation in a non-cell-autonomous manner and why petals are located at alternate positions from sepals. UFO orthologs are conserved in other flowering plants, including Antirrhinum and pea (Ingram et al., 1997; Taylor et al., 2001; Wilkinson et al., 2000). In Antirrhinum, the orthologous gene fimbriata (fim) is involved in the specification of organ positioning and the maintenance of organ boundaries (Ingram et al., 1997). This observation suggests that non-cell-autonomous regulation of petal initiation is conserved in flowering plants, a process mediated by a boundary gene, an F-box gene and a petal primordium gene.
Plant growth conditions
A. thaliana plants were grown in vermiculite in small pots under long-day conditions (16 h light/8 h dark) at 23-25°C. Arabidopsis ecotypes Col-0 and Landsberg erecta (Ler) were used as the wild types. The ptl-1, rbe-1 and ufo-6 mutants were from laboratory stocks, which were originally obtained from a stock center (https://abrc.osu.edu/; ufo-6) or were a gift from Prof. David Smyth (Monash University, Melbourne, Australia; ptl-1).
Plasmid construction and transformation
The PTL constructs generated in this study are shown in Fig. S1. To generate genomic fusions of PTL with Cyan Fluorescent Protein (SECFP), a 6.3-kb genomic fragment that included the PTL promoter and the coding sequence without the stop codon was amplified from Col-0 using 5′-CGGGATCCGATATCATTACGTGTTTGTCGGCA-3′ and 5′-GGGGTACCCTGATTCTCTTCTTTACTGAGCCT-3′ primers, digested by BamHI and KpnI, and ligated to pAN19 to generate pPTLpg19. The 1.2-kb PTL terminator was amplified using 5′-GGAGTCGAGCTCGTAATTTCTCTTAATGAAGAAGAA-3′ and 5′-CGGAATTCTCTAGACCAAATCAAGATCAAACA-3′ primers, digested by SacI and EcoRI, and cloned into pSECFP19, in which SECFP had been subcloned into the pAN19 vector, to generate pPTLtSECFP19. The BamHI and KpnI fragment from pPTLpg19 was subcloned into pPTLtSECFP19 to generate PTLp:PTLg::SECFP::PTLt (designed as PTLg:CFP). The PTLg:GR plasmid was constructed in the same manner, except that GR was used, which was amplified using 5′-GGGGTACCCAGCAAGCCACTGCAGGAGTC-3′ and 5′-GGAGTCGAGCTCTCATTTTTGATGAAACAGAAG-3′ primers, digested by KpnI and SacI, and cloned into pAN19. For PTLg:DTA, the SECFP region of PTLgCFP was replaced by the gene encoding DT-A, which was amplified using 5′-GGGGTACCATGGATCCTGATGATGTTGTT-3′ and 5′-CGCGAGCTCTTAGAGCTTTAAATCTCTGTA-3′ and digested with KpI and SacI to generate PTLg:DTA. NotI fragments of PTLg:CFP, PTLg:GR, and PTLg:DTA were subcloned into the pBIN30 binary vector. For CUC2g:GFP, the CUC2 promoter and coding sequence without the stop codon were amplified using 5′-GGGGACAACTTTGTATAGAAAAGTTGACTAGAGGAAGAGTTAAGAGATG-3′ and 5′-GGGGACTGCTTTTTTGTACAAACTTGCGTAGTTCCAAATACAGTCAAG-3′ primers and cloned into pDONR P4-P1R using the Gateway system (Invitrogen). The CUC2 terminator was amplified using 5′-GGGGACAGCTTTCTTGTACAAAGTGGCATCACAAAAGAGGTGACTTATA-3′ and 5′-GGGGACAACTTTGTATAATAAAGTTGAAATCATCTAACCGAAGATTCG-3′ and cloned into pDONR P2R-P3 (Invitrogen). These two plasmids, together with pDONR207 carrying GFP, were transferred to the pGWB multisite vector to generate CUC2p:CUC2-GFP::CUC2ter (CUC2g:GFP). The plasmids were transformed into Agrobacterium tumefaciens (Rhizobium radiobacter) strain GV3101 (pMP90) and into Col-0 by the floral dip method (Clough and Bent, 1998). GFP:RBEg and CUC1g:GFP plants were generated as described previously (Gonçalves et al., 2015; Takeda et al., 2014). The plants carrying two constructs were generated by crossing, and their genotypes were checked by PCR.
For fluorescence microscopy, inflorescences were trimmed, mounted on glass slides with water and examined under an LSM780 confocal microscope (Carl Zeiss). Flower images were captured with an SPAP0 binocular microscope equipped with an EC3 digital camera system (Leica).
DEX treatment and expression analysis using the PTL induction system
The PTLg:GR construct was transformed into the ptl-1 mutant. Twelve T1 lines showed resistance to 10 μg/ml glufosinate ammonium on selection medium (1% sucrose, 1% agar and 1×MS salt). Segregation and petal restoration were checked in the T2 generation. Seven lines showed restored petal development after treatment with DEX solution (10 μM dexamethasone in ethanol and 0.015% Silwet L-77). One homozygous line in the T3 generation was selected for further analysis. Mock treatment was performed with 0.1% ethanol and 0.015% Silwet L-77. After 3 h of DEX or mock treatment, total RNA was extracted from the inflorescences using an RNeasy Plant Mini Kit (QIAGEN). RNA integrity was confirmed using an Agilent RNA 6000 Nano Chip (Agilent Technologies). A library was constructed from a 0.5 µg RNA sample using an Illumina TruSeq Standard mRNA LT sample kit (Illumina). RNA sequencing was performed on the Illumina NextSeq 500 sequencing platform (Illumina), and data analysis was performed as described previously (Shimoki et al., 2021). We selected 42 and seven genes the expression of which was up- or downregulated, respectively, by summing those with a false discovery rate (FDR) <0.05, a sum (total number of mapped reads) >1, and a log2FC >1 (upregulated) or <−1 (downregulated).
For RT-qPCR, cDNA synthesis was performed using ReverTra Ace qPCR RT Master Mix with gDNA remover (Toyobo). The reaction was performed with Thunderbird SYBR qPCR mix (Toyobo) and a Dice Real Time System Lite thermal cycler (Takara Bio). The relative expression level was calculated based on amplification of the internal control gene TUB4 (At5g44340). The primer sequences are listed in Table S1.
mRNA in situ hybridization
Inflorescences from PTLg:GR plants were treated with DEX or mock for 3 h and fixed with formalin/acetic acid/ethanol (FAA). mRNA in situ hybridization was performed as previously described (Takeda et al., 2004). The UFO antisense probe was generated from the pDW221.1 template (Lee et al., 1997) and labeled using a DIG RNA labeling kit (Merck).
We thank Drs Michitaka Notaguchi, Takamasa Suzuki, Noriko Inada and Yoichiro Fukao, Ms Kaori Kaminoyama, Prof. Kiyotaka Okada and Prof. Detlef Weigel for providing materials, technical support and data analyses.
Author contributions
Conceptualization: S.T.; Methodology: S.T., Y.H., T.S., S.K., M.A., T.H.; Software: Y.H., T.S., S.K.; Validation: S.T., T.S., S.K., M.A., T.H.; Formal analysis: S.T., S.K.; Investigation: S.T., Y.H., T.S., S.K., M.A.; Resources: S.T.; Data curation: S.T., Y.H., T.S., S.K.; Writing - original draft: S.T.; Writing - review & editing: S.T.; Visualization: S.T., Y.H., T.H.; Supervision: T.H.; Project administration: S.T.; Funding acquisition: S.T., S.K.
This work was supported by Japan Society for the Promotion of Science KAKENHI grants (JP22570042 to S.T. and 21H02513 to S.K.) and the Supported Program for the Strategic Research Foundation at Private Universities from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (S1511023 to S.K.).
Data availability
RNA-sequencing data are available in the DBJJ Sequenced Read Archive under the accession number DRA013479.
Peer review history
The peer review history is available online at https://journals.biologists.com/dev/lookup/doi/10.1242/dev.200684.reviewer-comments.pdf.
Competing interests
The authors declare no competing or financial interests.


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If your actions don't live up to your words, you have nothing to say.
After awhile you realize that putting your actions where your mouth is makes you less likely to have to put your money where your mouth is.
Love is one of those topics that plenty of people try to write about but not enough try to do.
Prayer without work leads to the poor house


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Job Detail
Job ID 21854
Career Level Mid-Level
Experience 5 years
Qualifications BA/BSc/HND , MBA/MSc/MA
Job Description
Job Summary
The HR team Lead is responsible for the development and implementation of the workforce plan and ensuring the competitiveness of the organization within the marketplace as well as compliance with HR principles, policies and guidelines.
The ideal candidate is required to manage an effective and efficient HR function which delivers proactive and business focused advice on issues concerning HR in the company.
Job Duties/ Responsibilities/ Accountabilities
Oversee the completion of key administrative HR processes (e.g., staff contracts, holidays, general work permit issues payroll, benefits, relocation management, terminations etc.) to ensure effectiveness and accuracy in implementation and execution
Manage and oversee the company's recruitment process for key management positions
Ensure company compliance with labour regulations.
Provide counsel on HR perspectives and requirements related to all HR issues to managers and staff.
Develop corporate strategies that helps identify and research in-depth into human resources issues that impact on the company
Provide information, analysis, and recommendations to the company's strategic thinking and direction; establishing human resources objectives in line with the organizational objectives
Ensure effective implementation of the human resources strategies by establishing department accountabilities, including talent acquisition, staffing, employment processing, compensation, health and welfare benefits, training and development, records management, safety and health, succession planning
Oversee the recruiting, selecting, orienting, training, coaching, counselling, and disciplinary process for staff
Ensure adequate efforts are channelled into planning, monitoring, appraising, and reviewing staff job contributions; maintaining compensation; determining production, and productivity
Develop human resource financial strategies by estimating, forecasting, and anticipating requirements, trends, and variances; aligning monetary resources; developing action plans; measuring and analysing results; initiating corrective actions; and minimizing the impact of variances
Enforce compliance with federal, state, and local legal requirements by studying existing and new legislations; anticipating legislations; enforcing adherence to requirements; and advising management on required action points
Keep abreast with industry trends.
Administering Compensation and Benefit Plans
Gather and analyse data with useful HR metrics, like time to hire and employee turnover rates.
Supervise the maintenance of employee files and records in electronic and paper forms.
Represent the company in all external Human Resources Forum.
Minimum of Bachelor's Degree in Industrial Relations and Personnel Management, Human Resource Management, Sociology, Business Administration or business-related discipline
An MBA or relevant master's Degree in any business-related discipline
Relevant professional certifications such as Chartered Institute of Personnel Management of Nigeria (CIPM), Chartered Institute of Personnel and Development (CIPD), Society for Human Resource Management will be an advantage
Experience Required:
Minimum of 5 years post-graduation experience in Human Resources with at least 2 years in senior management positions.
TOP Skills Required:
In-depth knowledge of Local labor laws and regulations
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Personal development tools and methodologies
Training methods and evaluation
Performance management tools
Knowledge of Recruitment practices
Compensation and benefit computation
Payroll preparation and reporting
Microsoft office programs (Word, Excel, PowerPoint)
Leadership and People Management


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The Philadelphia beehive was not the only ones to receive a surprise last night (July 26) while in attendance at Beyonce's Mrs. Carter's Show Tour. While giving her final bows and recognition, Jay-z ran spontaneously ran out on the stage and gave the Bow Down singer a awkwardly sweet hug.
While the crowd went crazy, the spontaneous hug seemed to throw Mrs. Carter off balance and make her loose track of herself and the show. It takes her a few seconds to regroup but watch as she professionally pulls it back together and ends the show before running backstage to presumably give Mr. Carter a surprise of his own.


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VOTD: Steve Wozniak, Dan Kottke, And Andy Hertzfeld Dissect The Fact And Fiction Of 'Jobs'
Steve Jobs may not be around to tell us what's right or wrong about Jobs, but plenty of his former friends and colleagues are. That includes Apple co-founder Steve Wozniak, who's voiced his disappointment with the film's inaccuracies in the past.
Now, early Apple employee Daniel Kottke and Apple developer Andy Hertzfeld have joined Woz in an even more detailed two-hour discussion about the movie, hosted by Apple engineer John Vink. Hit the jump to find out how Woz actually got involved in the Homebrew Computer Club, what really led to Jobs's firing in 1985, and why they mostly think Pirates of Silicon Valley was the better Jobs film.
The panel picks over the film scene by scene, discussing what the show got right or wrong and explaining how things went down in real life. Unsurprisingly, they find that the movie conflated or flat-out invented certain events, shuffled around the chronology, and changed smaller details of bigger events.
One scene they took particular issue with was Jobs's departure from Apple. "The real situation was that the Mac failed terribly. Totally," Woz explained. "We built a factory to build 50,000 of them and we were selling 500 a month. Steve had cancelled projects because they could only sell 2,000 a month."
Woz also explained that a scene in which the movie's Jobs (played by Ashton Kutcher) has to drag the movie's version of Woz (played by Josh Gad) to the Homebrew Computer Club happened the opposite way in real life. "I pulled him to the club and showed him all the people around me," the real Woz said. "I'd been there since day one."
Nevertheless, Kottke, who served as a script consultant on Jobs, reported that the filmmakers "really did try very hard to get the history right," and believed that it "did a pretty good job of getting the emotional notes right" if not the actual events.
Hertzfeld seemed to disagree. "[The screenwriter] just had a superficial understanding," he said. "It was just a laundry list of incidents, as opposed to things that would show a deeper meaning."
Woz wasn't so enamored of the movie either, complaining that it didn't adequately show Jobs's thought process and charisma. The pair preferred Pirates of Silicon Valley, which they found to be just as well acted but much better written. For now, we can all just hope that Sony's version with screenwriter Aaron Sorkin will be better.


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Amazon Studios has acquired Aaron Sorkin's upcoming Lucille Ball biopic, in which Cate Blanchett is in talks to star as the late actress.
TheWrap reported two years ago that Blanchett was attached to star. Sorkin is penning the script, while Escape Artists Todd Black, Jason Blumenthal and Steve Tisch are producing.
This marks Amazon's second solo distribution title following last week's announcement that the company had acquired Woody Allen's "Wonder Wheel." For previous projects, Amazon Studios co-distributed with other companies. "The Big Sick," for example, was co-distributed with Lionsgate, while "Manchester By The Sea" was co-distributed with Roadside Attractions.
The film will chronicle Ball's 20-year marriage to Desi Arnaz, with whom she starred on the classic TV sitcom "I Love Lucy." Ball had two children with Arnaz before they divorced in 1960. She married Gary Morton the following year.
Blanchett won the first of two Academy Awards for playing another Hollywood icon, Katharine Hepburn, in Martin Scorsese's "The Aviator." She was also applauded for her performance in Todd Haynes' "Carol," for which she was nominated for a Best Actress Oscar.
She will next star in "Thor: Ragnarok," "Ocean's Eight" and "Where'd You Go, Bernadette?" She is represented by CAA and Wolf-Kasteler Public Relations.
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Sorkin last wrote the screenplay for "Steve Jobs" and is the writer and director on Jessica Chastain's "Molly's Game," which is looking at a release in November. He is represented by CAA and PMK*BNC.
No director has been announced as of yet for the project.


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The Life and Suffering of Sir Brante is Available Now on Xbox!
Hello, Xbox fans! We are pleased to announce that The Life and Suffering of Sir Brante is available on Xbox today! It is a narrative-based dark fantasy RPG where you can write your own story of Sir Brante.
The foundation for The Life and Suffering of Sir Brante was first laid in the fall of 2018, when the game designer and educator Fyodor Slusarchuk came up with the plan to transform the real-life role-playing production, set in the realm of the Blessed Arknian Empire, into a full-fledged video game.
The project would grow into a lifelong story that narrates the path of a single man born in a ruthless and unjust world. Following their alter ego from birth until true death, the player will grow up, make life-altering choices, experience exhilarating highs as well as terrifying lows, and endure social upheavals together with their character. The sheer scale of the game's core ideas dictated the choice of genre. Such a variety of events, heroes, and branching pathways for the player to take could only be brought to life in the form of a text-based, narrative-driven RPG.
The universe that Sir Brante inhabits had been invented before the work on the project even began. The Blessed Arknian Empire served as the setting for an entire series of real-life and tabletop RPGs. Its culture, religion, and history have now found a new incarnation in the world of video games. This strange and ruthless realm shall become the stage on which the tragic fate of Sir Brante will unfold.
This is a society where absolutism has reached its peak: the will of the ruling gods is unquestionable, and a commoner's lot is to toil away and suffer. Any transgression against the divine law entails a cruel punishment, both in life and after death. Yet even such a world cannot escape change, and the old era is poised on the verge of collapse. Growing up, your hero will inevitably play a part in these monumental events.
The game takes you on an adventure that starts at the very beginning of life — a little boy born in a dangerous medieval realm, split at the seams, governed by powerful nobles, and ruled over by the all-seeing Twin Gods. Live and grow alongside Sir Brante, dictate his choices and mistakes, and see it all unfold on the enchanting pages of the protagonist's journal.
Every choice made here matters — from breaking family bonds to rewriting the established laws of the universe. Where will you lead Sir Brante, and where shall his story take you? The choices are plenty, but the freedom to choose is yours alone. Endure great upheavals, encounter strife and love, push open and lock shut the doors to your own future!
Sir Brante's life story has received a large amount of positive feedback from users and the media since its release in March last year, and we are excited to present the project to Xbox players!
The Life and Suffering of Sir Brante