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BME103:T130 Group 12
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LAB 1 WRITE-UPInitial Machine TestingThe Open PCR machines is a DYI device that is composed of many circuit boards, wires, and a wooden frame. It is to be used to cycle DNA by oscillating the temperature of the DNA samples. This machine predominately works when the samples are placed in the main heating block, at which point a heated lid is placed down on top of the samples. Once the software for this Open PCR device is set up, the temperature change and the actual process begins. Within the Open PCR machine is a multitude of parts that keep the machine intact. These parts include a heat sink and fan to absorb heat, a circuit board that runs all the parts, a power supply to maintain the electricity, and a LCD display to show the user information. In conclusion, all of these parts work cohesively to generate this working machine known as the Open PCR. Experimenting With the Connections Test Run
The test run was done November 1st, 2012. Machine number 12 was used and there were minimal problems. It felt as if the machine was running slower than it should, but other than that all went well.
ProtocolsPolymerase Chain Reaction A polymerase chain reaction (PCR) is based on the enzyme DNA Polymerase's ability to synthesize complementary DNA strands. Through a series of steps involving polymerase breaking apart a DNA strand and then synthesizing a specified complementary piece, a PCR machine is able to isolate and amplify a desired strand of DNA.
Steps to Amplify a Patient's DNA Sample 1. PCR uses controlled temperature changes to make copies of DNA. Heat (about 95°C) separates double-stranded DNA into two single strands; this process is called denaturation. 2. "Primers", or short DNA strands, binds to the very end of the complimentary sequence that is being replicated. This step is called annealing, which takes place between 40°C and 65°C. The temperature that we used was 57°C. 3. Once the annealing process is done, the temperature is raised to about 72°C and DNA polymerase then extends from the primers copying the DNA. 4. PCR then amplifies a segment of a DNA sequence. In the end, there will be two new DNA strands identical to the original strand.
Components of PCR Master Mix • A modified form of the enzyme Taq DNA polymerase that lacks 5´→3´ exonuclease activity. • dNTPs • MgCl2 • Colorless Reaction Buffer (pH 8.5)
Components of PCR Master Mix
Sample 2: Patient ID: 11014 Age: 67 Gender: Male Replicate: 2 Sample 3: Patient ID: 11014 Age: 67 Gender: Male Replicate: 3 Sample 4: Patient ID: 46446 Age: 62 Gender: Female Replicate: 1 Sample 5: Patient ID: 46446 Age: 62 Gender: Female Replicate: 2 Sample 6: Patient ID: 46446 Age: 62 Gender: Female Replicate: 3 Sample 7: Positive Control Sample 8: Negative Control
1. To assemble the flourimeter, first obtain smartphone to capture the picture needed during data collection. 2. Turn on the flourimeter and drop a single drop of solution onto the hydrophobic slide. 3. Turn the black box provided upside down to cover the flourimeter. 4. Set up the smartphone on the stand provided, and align the camera/phone about 3 inches in front of the flourimeter. Make sure that the stand and the flourimeter is covered directly under the black box.
You will have 8 samples from the OpenPCR instrument and 1 DNA (calf thymus standard at 2 micrograms/mL) sample and water from the scintillation vial to analyze. 1. With a permanent marker, number your transfer pipettes at the bulbs so that you only use if for one sample. With the permanent marker number your Eppendorf tubes at the top. At the end, you should have 10 Eppendorf tubes and 10 pipettes clearly labeled. 2. Transfer each sample seperatly (using one pipette per sample) into an Eppendorf tube containing 400 mL of buffer. Label this tube with the number of your sample. Get your entire sample into this Eppendorf tube. You can use this sample number transfer pipette to place only this sample drop onto the fluorescent measuring device. 3. Take the specially labeled Eppendorf tube containing Sybr Green I using the specifically labeled pipette only place two drops on the first two centered drops as seen on the video. 4. Now take your diluted sample and place two drops on top of the Syber Green I solution drops. 5. Align the light going through the drop, as seen in the video. 6. Let the smart-phone operator take as many pictures using the light box as he/she wants. 7. Now either rerun the sample again or discard that sample's pipette. Keep the Sybr Green I labeled pipette. 8. You can run 5 samples per glass slide. 9. As the last sample run the water from the scintillation vial as a blank using the same procedure as with the other samples.
Our group used a Galaxy Nexus 1. After setting up the Flourimeter set a Smartphone's photo settings to the ones listed.
2. Once the samples have been prepared, place the Flourimeter in the light box. 3. Take as many pictures as needed. Your goal is to take pictures clear enough so ImageJ can take data from the images. 4. Once you have taken enough photos of that sample give the Flourimeter back to the sample preparer to prepare the next sample. 5. Repeat this procedure for all the samples.
1. ImageJ was used to analyze the images taken by the smartphone. To upload the image onto ImageJ, the ANALYZE tab was clicked and SET MEASUREMENTS was chosen. AREA INTEGRATED DENSITY and MEAN GREY VALUE was selected from the menu. 2. The MENU tab was selected and COLOR was chosen, the function SPLIT CHANNELS was used; three separate files were created. SYBR GREEN fluoresces green, so the image name with "green" next to it was used. 3. The oval selection was used to draw an oval around the green drop. Then, MEASURE was selected from the ANALYZE tab, and the sample number and the numbers measured from the image were recorded. 4. To get the readings from the background of the image, another oval of approximately the same size was drawn in the background green image and MEASURE was selected from ANALYZE tab. The sample number and the numbers measured from the image was recorded, this data will be labeled as "background". 5. The measurements were saved in an excel file by clicking SAVED AS from the FILE tab.
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology PCR detection works by heating the DNA sample to about 110°C in order to split the DNA. Then the PCR cools off to 57°C in order for the primer to attach to the DNA strands. The PCR then heats to 72°C so the DNA strand can be re-written. The rs17879961 cancer-associated sequence will produce a DNA signal because the reverse primer used, AACTCTTACACTCGATACAT(The letters in the sequence are the bases and stand for Guanine (G), Adenine (A), Cytosine (C), and Thymine (T). ) will only attach if the DNA sample has the same coding with the cancer-associated sequence "ACT". If the DNA sample does not have the cancer-associated sequence the primer will not attach because the sequence is AACTCTTACACTTCGATACAT, and there will be no DNA signal. The primer sequences that will be used is ACTC or in reverse CTCA. A positive result will be known because there will be a profound amount of the same sequence, the rs17879961. If there is none of the sequence than we know that the results are negative.
Basically Baye's Rule is that the results are not 100% accurate. Therefore, there will be some incorrect results. False positives and false negatives can occur often. Baye's Rule is what is used to find the false positive and false negative results. [IMG]http://i.imgur.com/XwQGA.png[/IMG] Source: http://openpcr.org/use-it/
ResultsBelow are the pictures that were put into ImageJ and used to gather data.


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Dough boxes are a great way to store dough for dinner rolls, pizzas, and more. They increase storage life by helping to prevent crusting on your dough. Keep in mind that if you use dough boxes specifically for proofing, your dough might rise more quickly than you'd like since plastic is a poor conductor of temperature. In addition to proofing, chefs use dough boxes to meet their portion control needs by storing pre-portioned rolls ahead of service.
Food Storage Boxes, Pails and Pans
Don't be confused by these seemingly interchangeable terms. Food boxes make it easy to stack and store foods due to their uniform design. Some food boxes even come with an added colander to make it easier to drain water from rinsed produce, or liquid from thawing protein. You'll find that food pails are similar to food boxes, but with one distinct design change: easy-to-carry handles. Another benefit of food pails? Multi-color options help you prevent cross-contamination when you follow these HACCP guidelines: red for raw meat (not fish or poultry), yellow for raw chicken, blue for raw fish, green for produce and white for dairy.
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Learn How to Shoot a Bow in a 7 Day Lesson in Brantley
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The most significant change has been the generation bubble that did archery in their youth and have grown up with the Lord of The Rings explosion into the worldwide mainstream of competitive sports and now sees it as a natural participation sport or art for their children as led by competitive athletes who are affiliated and instruct for our brand and web site.
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Brantley AL Archery Lessons
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Deadly suicide attacks in Afghanistan aimed at minority Shiite Muslims have experts wondering whether the war there could be taking a dangerous new sectarian turn.
Tuesday's twin attacks, in the capital of Kabul and the city of Mazar-i-Sharif, killed at least 60 Shiite worshippers as they celebrated Ashura, one of their sect's holiest days. It was the first such large-scale attack against Shiites in Afghanistan in more than a decade.
President Hamid Karzai called the attacks "unprecedented." A spokesman for Lashkar-e-Jhangvi, a Sunni extremist group based in Pakistan, claimed responsibility. The group has close ties to the Taliban and al-Qaida, as well as Pakistan's intelligence service, the ISI.
'The Seeds For Civil War'?
Experts are asking whether the attacks mean that anti-Shiite groups, which have carried out dozens of similar attacks in recent years inside Pakistan, are simply expanding their range. Alternately, Tuesday's suicide bombings could represent a larger strategy by the Taliban and its affiliated groups to turn the war into an Iraq-style sectarian conflict.
"I think you're creating the seeds for civil war," says Mark Sedra, a senior fellow at the Canada-based Centre for International Governance Innovation.
If sectarian and ethnic unrest spikes, he says, that could counter the argument that the United States and the international community will leave a stable Afghanistan behind in 2014.
Sedra doesn't believe the attack could have been carried out by Lashkar-e-Jhangvi without assistance from another group. Lashkar isn't known to have operated inside Afghanistan, he says. He thinks help came from either al-Qaida or the Taliban.
"These are the same sorts of attacks we saw in Iraq, targeting of Shia with the larger purpose of creating sectarian tensions," he says.
There are solidly drawn lines among ethnic and sectarian groups in Afghanistan, not just between Sunnis and Shiites, who are mainly ethnic Hazaras, but also among the Pashtuns, Uzbeks and Tajiks, as well as the various tribes.
"If we start to see some fracturing of existing alliances, if we start to see tensions emerging among these various groups, we might be setting the stage for a return to large-scale violence when the international forces leave," Sedra said.
The Taliban's History Of Persecuting Shiites
Vali Nasr, a professor at the Fletcher School of Law and Diplomacy at Tufts University, agrees that Tuesday's attacks could be the start of a new phase of the Afghanistan conflict.
"For a number of years, the emphasis was on fighting the United States and staying away from sectarianism, but right now that could be changing," Nasr says.
If the Taliban carried out the attacks against Shiites, who make up about 10 percent of Afghanistan's population, Nasr says that would be a return to the Tabiban's roots. He cites the massacre of an estimated 8,000 Hazaras after the Taliban captured Mazar-i-Sharif from its Shiite defenders in 1998.
"Anti-Shia sentiment is very much part of the very hard-line ideology of the Taliban," Nasr says. "It's the same sort of intellectual and ideological perspective that supports attacks on Shia in Pakistan."
Sparking sectarian violence "can easily spread to ethnic violence between the Pashtuns and the other ethnicities, and that adds new dimensions to the conflict," he adds.
Moving Out From Pakistan
Caroline Wadhams, a senior fellow at the Center for American Progress, describes Tuesday's violence as simply an "outlier" of the larger conflict.
Lashkar claimed responsibility for two earlier attacks — in 2009 on Ashura pilgrims that killed 30 people in Karachi, Pakistan, and the September killing of 29 Shiite pilgrims on a bus in Pakistan's Baluchistan province.
"The Pakistanis have been cracking down more on these groups, so maybe they're feeling pressure from that and have decided to move more into Afghanistan," Wadhams says.
She doesn't think the attacks on Tuesday would make strategic sense for the Taliban.
"Some of the attacks by the Haqqani network or [the Taliban], you can see a larger strategy at work and you can kind of understand the logic," she says. "This is just about killing out of hatred, out of an ideology. It doesn't seem to be more than that."
But Nasr sees Tuesday's violence as a signal.
"If you're a Hazara or a Tajik and you look at what happened to the Shias today, you see a warning sign," he says. "The Hazaras, the Uzbeks and the Tajiks are all going to be asking themselves, what happens when the Americans are gone? Who will protect them?"
For the Shiites in particular, the answer could be Iran, says Sedra.
"The Shias are intent on protecting themselves in any future Afghanistan, and they will likely have Iranian backing for that," he says. "This is going to make it more difficult to create the basic conditions for an orderly exit of U.S. and international forces."
Copyright 2021 NPR. To see more, visit https://www.npr.org.


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If you are a software developer who wants to build scalable AI-powered algorithms, you need to understand how to use the tools to build them. This Specialization will teach you best practices for using TensorFlow, a popular open-source framework for machine learning.
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The Machine Learning course and Deep Learning Specialization from Andrew Ng teach the most important and foundational principles of Machine Learning and Deep Learning. This new deeplearning.ai TensorFlow Specialization teaches you how to use TensorFlow to implement those principles so that you can start building and applying scalable models to real-world problems. To develop a deeper understanding of how neural networks work, we recommend that you take the Deep Learning Specialization.


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It's a story as old as Aristophanes' Lysistrata, the trope of a gender reversal that puts "women on top," but in a new book that's getting lots of attention, a journalist is trying to prove that this fiction has become a reality. In The End of Men: And the Rise of Women, Hanna Rosin argues that changes in the world economy have dramatically shifted gender roles. Women have adapted more skillfully to the new socioeconomic landscape by doggedly pursuing self-improvement opportunities, rebranding as the economy requires it, and above all possessing the kind of 21st century work attributes — such as strong communication skills, collaborative leadership and flexibility — that are nudging out the brawny, stuck-in-amber guys. Rock steadiness, long a cherished masculine trait, is about as useful in our fleet-footed economy as a flint arrowhead. Life favors the adapters, and it turns out they're more likely to be women.
(MORE: Judith Warner: We Need to Stop the 'Just-a-Mom' Routine)
Rosin is a gifted storyteller with a talent for ferreting out volumes of illustrative data, and she paints a compelling picture of the ways women are ascendant: women comprise the majority of undergraduate and graduate students on campuses nationwide and are outstripping men for professional degrees; newly employed single women are actually making more money than single men; women seem to have more ambition and a better ability to plan for the future. Moreover, as women gain more financial and cultural power, rates of teen pregnancy, violence and other bad outcomes have dropped. (There may be too much of a good thing: alarmingly, Rosin points to evidence that women may themselves become more violent toward men as they assume the cultural profile of traditional manhood.)
She's even sanguine about college hookup culture, claiming that young women are pretty happy with the crude status quo; they don't want to make any commitments that would jeopardize their career prospects and are willing to forgo what one forlorn undergraduate described as "someone to take me for a frozen yogurt" to avoid being trapped by an early marriage. We ourselves aren't so sure about this point since, from our perspective, the decline of college dating (which hasn't led automatically to marriage since the 1950s, by the way) denies both young women and men an opportunity to practice relationship skills they'll need in order to find long-term partners later. And it's hard to imagine why anyone tolerates the strikingly retro imbalance of sexual satisfaction (to wit: ability to reach orgasm) that accompanies casual hookup culture.
But while The End of Men captures a profound change in our cultural zeitgeist, one still has to wonder: Why are the relations between men and women still portrayed as a zero-sum game? Why do we need to establish who is winning and losing the war? So much of the coverage of gender issues — indeed the book's cover title itself — pits women and men against one another. In this way, Rosin, who mainly eschews biological explanations for gender differences, still cleaves to a binary characterization of men and women: when one is up, the other must be down. But history suggests that society is better served, and not only economically, when we see men and women engaged in cooperation rather than pitched battle.
When we see one sex as "on top," it makes it harder to recognize the downsides. American women are clearly having their "moment," but, as Rosin notes, their higher powers have resulted in bigger anxieties and more unhappiness than 40 years ago. That's a bitter pill for feminism. Rosin attributes the problem to women's inability to cede authority and delegate household responsibilities to their partners, but that explanation assumes they even have partners. We know that many women are single mothers, in precarious financial straits trying to keep their families afloat without a glimpse of any kind of man in the picture. They have achieved a new sense of autonomy, Rosin notes, that has undoubtedly contributed to declining levels of sexual violence. But they're hardly the fierce amazons we'd like to imagine.
Even the relatively equal and reportedly strong marriages of the educated class — the stable unions where financial and family responsibilities are traded off over the course of a marriage — show some chinks: experts put estimates of sexless marriages as high as 15% to 20%.
(MORE: Fifty Shades of Grey: Why Is It News That Women Like Sex?)
There is something else inescapable with this kind of dichotomy: if it is O.K. for women to be seen as superior in some regards, it's also O.K. for men to be seen as superior in others. That's not an argument about how women and men are "meant" to be, innately or in some ultimate, unchanging sense. But surely we can be honest with ourselves about what we see in front of us at a given moment in time, whether we are talking about rates of murder or rates of admission to graduate engineering programs. Former Harvard president Larry Summers was excoriated for his hypotheses about women and science and mathematics (mainly by people who had not carefully read his remarks), but one wonders if Summers had said something positive about women rather than men whether his remarks would have been noted at all. A world in which men and women are seen in opposition means that whenever we say a nice thing about one gender, we must be saying something bad about the other. But that is not the sort of world that helps us address complex problems, and it's not the sort of world most of us would want to inhabit.
MORE: Women, Money and Power


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John has photographs available for use in scientific / wildlife publications, thumbnails of which are shown here.
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He'll be the first to admit it, and he jokingly (I think) refers to Bear as Killer whenever he comes through the farm, which is fairly often since his father grew up here and his family has hunting cabins on the adjacent land (we own 240 acres, but the original property was bigger).
This guy is good.
Last night he curled up on a cozy pink blanket next to my side of the bed, using one of my socks for a pillow. Chewsticks and bones and toys, including the cool rope toy Joe made him, are scattered everywhere. For now, it's simply the safest place for him to be.
Those two were quite a handful, but they kept each other company and never came in the house because they needed to be brought up as working girls, not pets. (You can read about our losing Lottie and acquiring Daisy here.)
He is. He has a charming personality and has already become an integral part of the family. And of course he's as cute as can be. I know we were right to adopt him.
6/8/05: That Outfit Could Kill You
6/27/05: Chocolate Chip Sheep & Chocolate Chip Cookies
7/31/05: When? Soon (living on country time)
8/10/05: Whoa! Farm Visitors
4/12/06: Hearts and Rocks and Numbers and Thoughts
4/13/06: Shepherd's Nightmare
5/14/06: A Tiny Tail for Mother's Day (the story of baby Cary, who is now four)
6/15/06: How to Ensure a Happy Haying Crew
7/21/06: And Sheeeeeeee's SAFE!
7/18/06: The Tail of a Donkey and His Ratty Blue Halter
9/1/06: Happy Hour in the Garden
1/19/07: Merrily, Merrily, Merrily, Life is but a Stream
1/31/07: Sheep Shearing Early for a Change
2/3/07: Snowstorms and Sheep Shearing
5/13/07: The Tail of Two Mothers—A Mother's Day Story from the Farm
6/7/07: Farewell, New Cat
7/28/07: (It Only Looks Like) A Picture Perfect Walk in the Woods
2/3/08: Handyman Special
3/10/08: Broccoli Soup & Recharging Your Dead Batteries (Because Setting Them on Fire Isn't an Option)
3/13/08: Cary, No Baby
3/19/08: Flood Watch
4/20/08: Chick Days Are Here Again!
4/27/08: A Big Sunday Feast and a Mysterious Little Owl
9/18/08: Putting Up Hay and Losing Electricity
9/24/08: The Higher and Higher Cost of Farming
11/23/08: Heat Cheat
1/1/09: Happy Happy, New New
1/27/09: The Stuff of Farm Life—Losing Lambs and Lottie
5/6/09: Baby Cary is Three Years Old Today!
7/1/09: A Day in the Hay
9/19/09: Bye Bye Sheep Barn!
A Book, Building, and Bread Bakery Update
11/19/09: Rest in Peace, Little Donkey
Lambing Season 2006 Photos & Reports
Lambing Season 2006 Part 2
Lambing Season 2006 Part 3
Lambing Season 2007 Photos & Reports
Lambing Season 2007 Part 2
Lambing Season 2008 Part 1
Lambing Season 2008 Part 2
Lambing Season 2008 Part 3
Lambing Season 2009
Lambing Season 2009 Part 2
Lambing Season 2010 Part 1
Lambing Season 2010 Part 2
© FarmgirlFare.com, the young at heart foodie farm blog where after two weeks, the consensus around here is that Bert has more energy than the rest of us put together. Puppies!


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Do you hear a ringing, clicking or hissing sound in your ears?
Is it constant or does it come and go?
Does this noise bother you or impair your daily function?
If you have experienced any of these, you are not alone. Tinnitus is commonly described as a ringing, buzzing, whooshing or hissing sensation in the ears, but the sound isn't caused by an external noise. More than 50 million Americans experience tinnitus for a number of different reasons. While it won't cause deafness, tinnitus can affect your daily activities. To determine how to best treat tinnitus, it's important to understand its symptoms and causes.
There are a number of different symptoms for tinnitus, but the most common include hearing the following sounds:
- Ringing
- Buzzing
- Whooshing
- Hissing
- Clicking
These sounds are heard only by the person experiencing the tinnitus, but can sometimes be detected by a hearing professional. These sounds can be heard constantly or intermittently. Some people have problems sleeping when they have tinnitus because they feel as though their brain never "shuts off."
There are several factors that can cause tinnitus, including the following:
- Exposure to loud noise
- Medication
- Hearing loss
- Ear infection
- Trauma to the ear
- Earwax buildup
- Meniere's disease
- Vascular disorders
Tinnitus may be caused by one or more of these conditions and some can be resolved easily if the cause is temporary, such as an infection or a buildup of earwax.
Depending on the cause of your tinnitus, there are a number of different treatment methods available to help you find relief. Your hearing care provider will assess the severity of your tinnitus, symptoms, how it impedes your daily life and possible causes in order to recommend one of the following solutions.
- Address the problem: If your tinnitus is due to earwax build up, medication or infection, they'll recommend the proper course to resolve the issue. This may include removing the earwax, switching medications or antibiotics. Behavioral therapy may also be recommended to help treat the varying emotions you may experience with tinnitus, from anxiety to anger.
- Hearing aids: Hearing devices are incredibly helpful in treating tinnitus. Even if a hearing loss isn't present, hearing aids can be equipped with tinnitus-masking features that help cover the tinnitus and offer relief from the unwanted noise.
- White noise machines: If your tinnitus isn't as severe or is only experienced at night time, a white noise machine may be ideal for helping you find relief. White noise machines produce a steady sound to help mask the tinnitus noises you hear.
- Tinnitus retraining therapy (TRT): TRT is a form of therapy designed to help individuals with tinnitus find relief. This therapy involves retraining your mind to block out and hear certain tones, which in turn, helps to ease the symptoms caused by tinnitus.
If you are suffering from tinnitus, schedule an appointment with a hearing professional to be evaluated. The hearing provider will work to identify the cause behind your tinnitus and recommend the appropriate treatment.


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Fans – Review Creators
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Eve Minax is a Adult Content Model-focused influencer and content creator, inspiring and entertaining their audience with their unique perspective and captivating personality. With a passion for their craft, Eve Minax has gained a significant following and has become a trusted voice in the Adult Content Model social media community.
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Chapter 2
Redemption comes through the Holy Messiah—Freedom of choice (agency) is essential to existence and progression—Adam fell that men might be—Men are free to choose liberty and eternal life. About 588–570 B.C.
1 And now, Jacob, I speak unto you: Thou art my afirstborn in the days of my tribulation in the wilderness. And behold, in thy childhood thou hast suffered afflictions and much sorrow, because of the rudeness of thy brethren.
2 Nevertheless, Jacob, my firstborn in the wilderness, thou knowest the greatness of God; and he shall consecrate thine aafflictions for thy gain.
3 Wherefore, thy soul shall be blessed, and thou shalt dwell safely with thy brother, Nephi; and thy days shall be aspent in the service of thy God. Wherefore, I know that thou art redeemed, because of the righteousness of thy Redeemer; for thou hast bbeheld that in the cfulness of time he cometh to bring salvation unto men.
4 And thou hast abeheld in thy youth his glory; wherefore, thou art blessed even as they unto whom he shall minister in the flesh; for the Spirit is the same, yesterday, today, and forever. And the way is prepared from the fall of man, and bsalvation is cfree.
5 And men are instructed sufficiently that they aknow good from evil. And the blaw is given unto men. And by the law no flesh is cjustified; or, by the law men are dcut off. Yea, by the temporal law they were cut off; and also, by the spiritual law they perish from that which is good, and become miserable forever.
6 Wherefore, aredemption cometh in and through the bHoly cMessiah; for he is full of dgrace and truth.
7 Behold, he offereth himself a asacrifice for sin, to answer the ends of the law, unto all those who have a broken heart and a contrite spirit; and unto bnone else can the cends of the law be answered.
8 Wherefore, how great the importance to make these things known unto the inhabitants of the earth, that they may know that there is no flesh that can dwell in the presence of God, asave it be through the merits, and mercy, and grace of the Holy Messiah, who blayeth down his life according to the flesh, and taketh it again by the power of the Spirit, that he may bring to pass the cresurrection of the dead, being the first that should rise.
9 Wherefore, he is the firstfruits unto God, inasmuch as he shall make aintercession for all the children of men; and they that believe in him shall be saved.
10 And because of the intercession for aall, all men come unto God; wherefore, they stand in the presence of him, to be bjudged of him according to the truth and choliness which is in him. Wherefore, the ends of the law which the Holy One hath given, unto the inflicting of the dpunishment which is affixed, which punishment that is affixed is in opposition to that of the happiness which is affixed, to answer the ends of the eatonement—
11 For it must needs be, that there is an aopposition in all things. If not so, my firstborn in the wilderness, righteousness could not be brought to pass, neither wickedness, neither holiness nor misery, neither good nor bad. Wherefore, all things must needs be a compound in one; wherefore, if it should be one body it must needs remain as dead, having no life neither death, nor corruption nor incorruption, happiness nor misery, neither sense nor insensibility.
12 Wherefore, it must needs have been created for a thing of naught; wherefore there would have been no apurpose in the end of its creation. Wherefore, this thing must needs destroy the wisdom of God and his eternal purposes, and also the power, and the mercy, and the bjustice of God.
13 And if ye shall say there is ano law, ye shall also say there is no sin. If ye shall say there is no sin, ye shall also say there is no righteousness. And if there be no righteousness there be no happiness. And if there be no righteousness nor happiness there be no punishment nor misery. And if these things are not bthere is no God. And if there is no God we are not, neither the earth; for there could have been no creation of things, neither to act nor to be acted upon; wherefore, all things must have vanished away.
14 And now, my sons, I speak unto you these things for your profit and alearning; for there is a God, and he hath bcreated all things, both the heavens and the earth, and all things that in them are, both things to act and things to be cacted upon.
15 And to bring about his eternal apurposes in the end of man, after he had bcreated our first parents, and the beasts of the field and the cfowls of the air, and in fine, all things which are created, it must needs be that there was an opposition; even the dforbidden efruit in fopposition to the gtree of life; the one being sweet and the other bitter.
16 Wherefore, the Lord God gave unto man that he should aact for himself. Wherefore, man could not bact for himself save it should be that he was centiced by the one or the other.
17 And I, Lehi, according to the things which I have read, must needs suppose that an aangel of God, according to that which is written, had bfallen from heaven; wherefore, he became a cdevil, having sought that which was evil before God.
18 And because he had fallen from heaven, and had become miserable forever, he asought also the misery of all mankind. Wherefore, he said unto Eve, yea, even that old serpent, who is the devil, who is the father of all blies, wherefore he said: Partake of the forbidden fruit, and ye shall not die, but ye shall be as God, cknowing good and evil.
19 And after Adam and Eve had apartaken of the forbidden fruit they were driven out of the garden of bEden, to till the earth.
20 And they have brought forth children; yea, even the afamily of all the earth.
21 And the days of the children of amen were prolonged, according to the bwill of God, that they might crepent while in the flesh; wherefore, their state became a state of dprobation, and their time was lengthened, according to the commandments which the Lord God gave unto the children of men. For he gave commandment that all men must repent; for he showed unto all men that they were elost, because of the transgression of their parents.
22 And now, behold, if Adam had not transgressed he would not have fallen, but he would have remained in the garden of Eden. And all things which were created must have remained in the same state in which they were after they were created; and they must have remained forever, and had no end.
23 And they would have had no achildren; wherefore they would have remained in a state of innocence, having no bjoy, for they knew no misery; doing no good, for they knew no csin.
24 But behold, all things have been done in the wisdom of him who aknoweth all things.
25 aAdam bfell that men might be; and men care, that they might have djoy.
26 And the aMessiah cometh in the fulness of time, that he may bredeem the children of men from the fall. And because that they are credeemed from the fall they have become dfree forever, knowing good from evil; to act for themselves and not to be acted upon, save it be by the punishment of the elaw at the great and last day, according to the commandments which God hath given.
27 Wherefore, men are afree according to the bflesh; and call things are dgiven them which are expedient unto man. And they are free to echoose fliberty and eternal glife, through the great Mediator of all men, or to choose captivity and death, according to the captivity and power of the devil; for he seeketh that all men might be hmiserable like unto himself.
28 And now, my sons, I would that ye should look to the great aMediator, and hearken unto his great commandments; and be faithful unto his words, and choose eternal life, according to the will of his Holy Spirit;
29 And not choose eternal death, according to the will of the flesh and the aevil which is therein, which giveth the spirit of the devil power to bcaptivate, to bring you down to chell, that he may reign over you in his own kingdom.
30 I have spoken these few words unto you all, my sons, in the last days of my probation; and I have chosen the good part, according to the words of the prophet. And I have none other object save it be the everlasting awelfare of your souls. Amen.


text: Animal hospitals and clinics deal with the treatment of animals or pets. They also provide pet training tips, information on pet grooming, as well as immunization of pets from different animal diseases and infections, done by a veterinary doctor. In Teigen, animal hospitals meet stringent professional regulations and are subject to regular inspections of their property and records. Most veterinarian hospitals or clinics in Teigen offer low-cost services at least once a year in conjunction with a local animal shelter or other worthy cause. These clinics are usually just small-animal vaccination opportunities. In Teigen, even some animal hospitals act as rescue groups for a discount program. Most of the rescue groups' members also come to the hospital with their own pets. A few clinics provide free sterilization, vaccination and flea treatment and prevention, with the aid of volunteer animal hospitals. Some registered animal hospitals, work round the clock to respond to emergencies, even provide vets on a 24-hour service. There are some hospitals that treat stray and wild animals that are brought by their field staff, such as inspectors and animal welfare officers.


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When you think of Nintendo's hit products, you don't necessarily think of the Mario Kart racing games (specifically) as a dominant part of the 3DS maker's business. But the series is colossal. Mario Kart Wii has outsold every other standalone game on the home system, moving a staggering 28 million copies. That's one game for every three Wii consoles.
Any other publisher would put out one of these every year; Nintendo does one per console and then sells it for half a decade. So the development team generally plays it safe, as is the case with this 3DS installment, to be released on December 4. Now, when Nintendo played it safe with the Wii version, it crafted something amenable to the console's fan base: a madcap fun-for-all in which anyone including your triplegic maiden aunt Tillie had an equal chance of winning the race. (She and her 28 million friends say I was wrong to dislike it.)
But nowadays Tillie is too busy playing another round of Pissed-Off Parakeets on her iPhone 4S to bother buying a 3DS. So this time, "playing it safe" means embracing the early-adopter hardcore gamers. This has resulted in a Mario Kart more tuned to our sensibilities, which is just as well since Tillie can barely see the 3-D graphics what with her glaucoma.
The Mario Kart series may appear at first blush to be racing games, but in fact they are a carefully crafted videogame version of Harrison Bergeron. You can't simply outrace the pack of Mario characters through the 32 different cartoon courses. You have to use items to attack them and defend yourself. The Handicapper General distributes the items, giving lead racers the crappy items (a tiny banana peel that you can drop on the track in hopes that someone will at some point be dumb enough to drive over it) and the racers in the back get shitkicker death cannons.
Here is a typical scenario: you are in a tenuous first place, just barely holding off the guy in second, carefully considering your racing line and drifting corners to edge him out. Suddenly, the last place person accidentally drives into an item box and fires off a Blue Shell, which zooms out to the front of the pack and explodes on you for the crime of doing slightly better than the guy right behind you. You go out in a blaze of glory and second place zooms into first place. The guy who fired the shell? He's still in last, of course, but he gets to hear you scream some unrepeatable words, and isn't that fun for everyone? Except you, but who cares about you.
Anyway, the basic formula is still in place in Mario Kart 7 but it has been toned down considerably. It's possible, both against the computer racers and against other humans, to get far enough ahead that you can maintain first place after a Blue Shell bombing, which is better than nothing. I've still lost a fair few races I should have won (and vice-versa) but it all felt a lot more fair.
That's good, because Mario Kart 7's pure racing can stand on its own. The controls are tight. The gameplay looks simple but has a lot of depth: you can drag items behind you as shields, tap a button to get a burst of speed every time you catch air on the undulating race tracks, etc. Yes, it feels pretty much exactly like 2005's Mario Kart DS with better graphics. But it's still too good to put down.
Silent friends
Mario Kart 7 has online multiplayer, Nintendo-style, which means you can neither speak with or send texts to the people you are racing for fear that you may say something unsanitary. Given the language that I have used in front of Nintendo employees at various game preview events, I am probably the reason for this rule and I do apologize. It still seems a bit like swatting a fly with a sledgehammer.
There are other ways to customize your experience. You can tweak your go-kart's frame, wheels and glider (used to float down speedily off the courses' bigger jumps). You can form a "community" of like-minded racers and adjust the game modes and the items that appear, although you can only pick from general categories ("Shells Only," "No Items," et cetera) rather than individually turn items on and off.
After getting ruined in the Wii game, Battle Mode is mostly back in fighting form for Mario Kart 7. You still zip around a wide-open arena firing weapons at your friends. But while previous games gave each player three hit points and the winner was the last man standing, the Handicapper General has now decreed that everyone must always get to play for the entire match. So players now score points for hitting others, and lose them for taking too many hits. This is actually a good idea, but it would have been better had it been an additional option rather than the only one.
The actual new addition, Coin Runners, is also a good idea: The aim is to putter around the battlefield collecting up coins, which can then be extricated forcibly from other players by blowing them up real good. It's not an original idea (I'm pretty sure I played something similar in Halo: Reach) but it's a rare breath of fresh air.
Which Mario Kart sometimes seems to be gasping for. In the latest edition of Iwata Asks, the regular series in which Nintendo's CEO quizzes his employees on the development cycles of their latest games, Mario Kart's team is clear that this was not an "it's done when it's done" vanity project: This game had to be out in 2011 as part of the company's all-guns-blazing plan to kickstart the 3DS business. In other words, there was no time to dream too big even if they wanted to.
That said, if a Nintendo with its back to the wall produces games that are more tuned to core gamers' sensibilities, I'll take it.
"Use a TOYin3D viewer to watch in 3D this pictures & trailer on your mobile"
3D Screenshot:
3D Trailer:
The good:
- Clever course design
- Tight racing control
- Easy to learn but has lots of depth
- Fixed Battle mode (mostly).
The bad:
- Nintendo's bare-bones online
- General play-it-safe design mentality.
The ugly
- Blue shells.
Source [arstechnica.com]


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Country of My SkullEver since Nelson Mandela dramatically walked out of prison in 1990 after twenty-seven years behind bars, South Africa has been undergoing a radical transformation. In one of the most miraculous events of the century, the oppressive system of apartheid was dismantled. But how could this country - one of spectacular beauty and promise - come to terms with its ugly past? How could its people, whom the oppressive white government had pitted against one another, live side by side as friends and neighbors? To begin the healing process, Nelson Mandela created the Truth and Reconciliation Commission, headed by the renowned cleric Archbishop Desmond Tutu. Established in 1995, the commission faced the awesome task of hearing the testimony of the victims of apartheid as well as the oppressors. In this book, Antjie Krog, a South African journalist and poet who has covered the work of the commission, recounts the drama, the horrors, the wrenching personal stories of the victims and their families. Through the testimonies of victims of abuse and violence, from the appearance of Winnie Mandela to former South African president P.W. Botha's extraordinary courthouse press conference, this award-winning poet leads us on an amazing journey. |
They Never Wept the Men of My Race | 1 |
None More Parted than Us | 14 |
First Hearings | 22 |
Copyright | |
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Afrikaner already amnesty answer Apartheid asked become blood body Botha bring busy called changed child coming Commissioners Committee crying death don't door eyes face fact father feel fire force four front give Government hall hand happened head heard hearing heart honour human it's journalists keep kill kind later leader leave live look Mamasela Mandela means months morning mother murder never night offices Party past person police political questions reconciliation remember responsibility says shot someone sound South African speak stand started Stompie stop story taken talk tell things thought told took town Truth Commission turned Tutu victims voice waiting walk week whole wife woman wrong


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Job Summary As a key member of the team, this position is responsible for development and implementing a marketing and communications strategy. The Team Leader is... Full Description


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How old were you when you stopped receiving Easter baskets? I'm proud to say I was 18, and not an Easter goes by where I don't miss those hollow chocolate bunnies, Peeps, little nail polishes and a $20 bill tucked into the fake grass. The thing I love about being all grown up now, though, is that I know how to make my own Easter candy!
Chocolate turtles are one of my favorite sweet treats ever. The creamy caramel mixed with crunchy pecans (or in this case, walnuts), coated with a sweet, decadent layer of milk chocolate… mmm! Against turtles I am powerless.
They're actually pretty simple to make, too. Just arrange the nuts on a baking sheet, make your caramel and pour it over. Wait while it hardens a little, then drip a tablespoon of melted chocolate over each little cluster. Once the candy is set, you're ready to munch away!
If you prefer pecans, use pecans. If you prefer dark chocolate, go right ahead. Make them to your taste! Sprinkle on some fleur de sel, melt them and serve them over ice cream, give them away to friends and family (if you have the self control). Or do like I do, and share with no one! Just kidding. I gave my husband a bite.
Chocolate, Caramel and Walnut Turtles
- 4 tablespoons butter, cut up, plus more for pans
- 3/4 pound walnut halves, about 3 1/2 cups, toasted
- 1 cup evaporated milk
- 1 cup light corn syrup
- 1 cup sugar
- 1 teaspoon vanilla
- 1/2 pound semisweet chocolate, chopped
- 1 tablespoon vegetable shortening, recommended: Crisco
Equipment needed:
- Candy thermometer
- Generously butter 2 baking sheets. On the baking sheets, arrange the nut halves in snowflake-shaped clusters, leaving 2 inches of space between each.
- In a medium saucepan, combine 1/2 cup of the evaporated milk, the corn syrup, and sugar and bring to a boil. Add the remaining 1/2 cup evaporated milk and the 4 tablespoons butter, and cook, stirring constantly, until it reaches the soft ball stage (as noted on thermometer), 240 degrees F. Stir in the vanilla and remove from the heat. Let cool to 200 degrees F.
- Using a tablespoon, spoon caramel on top of each nut cluster and let harden. (If the caramel becomes too stiff to pour, return to low heat, and cook, stirring constantly, until it returns to the proper consistency.)
- To make the chocolate coating, in the top of a double boiler or in a metal bowl set over barely simmering water, combine 6 ounces of the chocolate and the shortening. Melt over low heat, stirring, until it reaches 116 to 118 degrees F, or feels fairly warm but not hot to the touch.
- Remove from the heat and stir in the remaining 2 ounces of chocolate. Continue stirring until the chocolate reaches 80 degrees F or feels cool to the touch. Return to low heat and cook, stirring, until the temperature rises to 85 to 87 degrees F, or feels barely cool. Remove from the heat.
- Drizzle 1 tablespoon of tempered chocolate over each cluster. Set aside in a cool place to harden. Store in an airtight container at room temperature for up to 2 weeks, with waxed paper separating the layers to prevent sticking.
- Enjoy!


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India may have just gotten the wake-up call it needed to start getting serious about its growing pollution problem. A study put out this week by Tel Aviv University reports that Indian megacities are seeing a faster increase in pollution than the cities in the classic environmental villain to the east, China. According to the paper, from 2002 to 2010, Bangalore saw the second highest increase in air-pollution levels in the world at 34%, and Indian cities including Pune, Mumbai, Nagpur and Ahmedabad, among others, also saw double-digit increases. Because the pollution measured reflects a combination of industrial and weather-related particles like dust, it's not possible to say this is entirely a man-made problem. But the fact that India's population grew faster than China's in the past decade means that the air in India's cities is doomed to get worse before it gets better.
It doesn't seem fair that China gets both the job-supplying manufacturing sector and the relatively better pollution news. (It's increasing in Chinese cities too — just not as fast.) But no one in India could claim the information to be much of a shock. Though this is my first winter in New Delhi, everyone keeps telling me that it's "normal" this time of year for the Indian capital to get smoggy. But how smoggy is normal? So smoggy that companies spam my phone with texts for Ayurvedic asthma cures? Apparently so, because I've gotten them. When winter kicks in, the cooler temperatures and still air always bring an annual period of haze to the city, but the pervasive murk that enshrouded the city for the first few weeks of November raised more concerns than usual, and not just mine. According to researchers at India's Centre for Science and Environment, levels of respirable particulate matter (PM10) in mid-November were up 47% over what they were that time last year, and nitrogen-dioxide levels were up 57%.
(MORE: Serious Air Pollution in Beijing Is Leading to Social Unrest)
Officials have been on the defense, and they should be. New Delhi Chief Minister Sheila Dikshit announced the state had commissioned one of India's best universities to study the causes of this year's bad smog, in addition to proposing a series of quick fixes to help clear the air, like restricting diesel-generator use and banning trucks not bound for New Delhi from entering the city. She also has blamed the burning of straw in neighboring states of Haryana and Punjab, a claim that officials there dismissed, correctly pointing out that if that were the problem, they'd be covered in smog too.
The gray cocoon that enshrouded the city earlier in November is at bay for now, which means we can see the sky. That's a nice change. But it'll be back. In a study released earlier this year, India ranked last out of 132 countries surveyed by Yale and Columbia in terms of air quality and effects on human health. Because of its high-emissions vehicles and heavy reliance on coal, the country has particularly serious levels of high-particulate matter, which leads to respiratory infections, and the problem is seen both in cities like New Delhi and smaller cities around the nation.
"You always have an increase in respiratory cases this time of the year, partly because of the worsening air quality and partly because of the seasonal changes," says Dr. S.K. Chhabra, head of the Department of Cardiorespiratory Physiology at the Vallabhbhai Patel Chest Institute in New Delhi. He says he's been seeing more asthma and bronchitis patients in the past few weeks, as well more cardiac patients with angina, which is aggravated by the lowered oxygen levels during heavy pollution. People with pre-existing lung or heart disease, smokers, young children and the elderly are particularly susceptible. "When the wind starts again, there will be less cases."
(PHOTOS: New Delhi Demands Water: The Indian Capital's Annual Shortage)
That doesn't make it O.K. The New Delhi government has taken steps in the past that have helped control the city's pollution levels, like rolling out vehicles that run on natural gas to reduce emissions, building a metro system to encourage public transportation and limiting the amount of commercial traffic coming through town. Last week, the Centre for Science and Environment called on officials to do much more, including enforcing emissions checks on private and commercial vehicles, banning open fires in the city and using a smog-alert system to let citizens know when the pollution is particularly risky. Earlier this month, the city announced it would expand monitoring, install electronic displays charting air quality around the city and issue smog alerts and health advisories, according to the Hindustan Times.
Those are steps that have a proven track record in other cities. Growing up in the 1980s in Los Angeles, we used to have regular "smog days," when public schools would cancel our physical-education classes because the city advised it was unhealthy for kids to be outside. (Some amazing pictures of the bad old days in L.A. here.) L.A. has mostly cleaned up its act through a combination of citizen activism, better technology and better regulation. The alerts were good public-health practice, sure, but they did more than that. They were a reminder that heavy air pollution, even if it was the norm, was not normal. They are also a reminder to citizens that if officials aren't doing all they can to get things back to normal, they're not doing their job. If India's private sector can send me regular text messages about Ayurvedic cures for asthma, surely I could get a courtesy note from the government I pay taxes to when air-pollution levels might give me an asthma attack.


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