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1Ethiopian Sugar Corporation Research and Training Division, Biotechnology Research Team, Wonji Research Center, P.O Box 15, Wonji, Ethiopia
2National Agricultural Biotechnology Research Program Holetta Agricultural Research Center
3Jimma University College of Agriculture and Veterinary Medicine, Jimma, Ethiopia
Received date: January 19, 2016; Accepted date: February 04, 2016; Published date: February 11, 2016
Citation: Tesfa M, Admassu B, Bantte K (2016) In Vitro Rooting and Acclimatization of Micropropagated Elite Sugarcane (Saccharum officinarum L.)Genotypes - N52 and N53. J Tissue Sci Eng 7:164. doi:10.4172/2157-7552.1000164
Copyright: © 2016 Tesfa M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Availability of sufficient quantity and quality of sugarcane planting materials from conventional seed source is one of the major challenges in the Ethiopian sugar estates. To circumvent this challenge, tissue culture technology is found to be the best alternative for which in vitro propagation protocol is a key pre-request. Thus, the present study was aimed to optimize protocol for in vitro rooting and acclimatization of two elite sugarcane genotypes i.e., N52 and N53. Experiments were laid out in a completely randomized design with factorial treatment arrangements. Half strength MS liquid media supplemented with combination of Sucrose (0, 40, 50, 60 and 70 g/l) and NAA (0,3,5 and 7 mg/l) along with two sugarcane genotypes (N52, N53) were used for rooting while substrate containing sand, soil and farmyard manure in six different ratios (1:1:0, 1:1:1, 1:2:1, 2:1:1, 1:1:2 and 1:2:0) were used for acclimatization. With regard to in vitro rooting, ½ strength liquid MS medium + 50 g/l sucrose + 3 mg/l NAA induced the highest rooting (100%) with 23.5 ± 1.29 average root number per shoot and 4.95 cm ± 0.06 cm root length in genotype N52 while 5 mg/l NAA + 50 g/l sucrose induced the highest (100%) rooting response with an average of 21.76 ± 0.57 root number per shoot with 4.54 cm ± 0.06 cm root length in sugarcane genotype N53. In acclimatization, best survival rate (94% in N52 and 100% in N53) was achieved on substrate mixtures containing sand + soil in 1:1: ratios. Thus, it can be deduced that this protocol can be used successfully for in vitro rooting and acclimatization of these genotypes.
In vitro rooting; Acclimatization; Liquid medium; NAA; Sucrose; N52 and N53.
Sugarcane (Saccharum officinarum L.) is an important industrial cash crop of Ethiopia. It was introduced to the country in the 16th century [1]. However, commercial sugarcane production was commenced in 1952 by Dutch company, Handles-Vereening Amsterdam (HVA) at Wonji shoa sugar factory [2]. Currently, it is cultivated in three sugar factories over an area of 37, 000 ha with total production of 300,000 ton of sugar and 11.1 million liters of ethanol annually [3], which accounts 100% of Ethiopian sugar production [4]. However, most of the sugarcane plantations of sugar industries are confronting with problems of low cane yields due to poor quality seed material, prevalence of diseases and pests, slow commercialization of improved sugarcane varieties. Therefore, to alleviate the problems, use of efficient propagation system is a must for mass multiplication of disease free seed material of improved sugarcane varieties in a short period.
Plant tissue culture is currently a powerful tool that plays a major role in rapid multiplication of disease free planting material of newly improved varieties through in vitro technique on continuous year rounded basis [5]. Shoot tip culture is relatively simple in vitro method for rapid propagation of selected sugarcane materials and the clean or pathogen free plant materials [6]. In line with this, protocols have been developed for rapid multiplication of newly released and commercially important genotypes of sugarcane through shoot tip cultures in Ethiopia [4,7,8]. The ultimate success of commercial micropropagation depends largely on successful rooting and acclimatization of in vitro derived plantlets. Rooting process is genetically determined and influenced by culture environment factors such as mineral concentration, type and concentration of growth regulators and sucrose concentration in the culture media. Thus genotype specific protocol is needed as the plant growth regulators and sucrose requirements for morphogenetic responses of in vitro rooting vary from genotype to genotype in sugarcane [5,9].
Similarly, in the acclimatization stage, it is necessary to ensure optimal ex vitro conditions to obtain high survival rates. The selection of a suitable substrate is one of a decisive factor which substantially affects the survival rate, growth and development of ex vitro acclimatized plantlets and mainly depends on plant species. Although, in acclimatization of micropropagated plantlets on suitable media. Therefore, the study was carried out with objectives to optimize in vitro rooting and acclimatization protocol for two elite sugarcane genotypes.
The study was conducted at the National Agricultural Biotechnology Laboratory of the Ethiopian Institute of Agricultural Research, in Holetta. Two elite sugarcane genotypes (N52 and N53) were used for the study. The genotypes were obtained from the Ethiopian Sugar Corporation, Research and Training Division and they were selected based on their cane yield performance and sugar quality. The planting material (setts) with two buds were treated with hot water at 50°C for 2 hours followed by immersing in fungicide (Bayleton® DF 50%) solution at rate of 1 g/l for 5 minutes. The treated setts were planted in plastic pots containing mixture of autoclaved forest soil, farm yard manure and river sand in the ratio of 1:1:1 and allowed to grow in a screen house for five months. Actively growing shoot tops were used as source of shoot tip explant. The method of explant preparation and surface sterilization was adopted from [7,10] with some modifications. Actively growing shoot tops were taken from 5-months-old screen house grown healthy mother plants and used as explants. The entire leaves were removed, and the segments were taken to the laboratory for surface sterilization and explant preparation. Trimmed shoot tops were washed thoroughly under running tap water for 30 minutes, and resized to 10 cm length by cutting off at the two ends. Then the explants were further washed for 30 minutes with tap water containing a drop of liquid detergent solution plus two drops of tween-20 with continuous shaking and rinsed three times with double distilled water. Later, the explant was taken to a laminar air flow cabinet and immersed in 0.1% (w/v) Bavistin® DF 50% (Carbendizem) fungicide solution, ascorbic acid (0.2% w/v) and citric acid (0.4% w/v) for 30 minutes followed by three times rinsing each for five minutes with sterile double distilled water. The shoot tips were washed again with 70% ethanol for one minute and rinsed with sterile double distilled water three times each for five minute to remove residual ethanol from the shoot tip surface. Finally, 10 cm sized explants were surface sterilized with 50% (v/v) aqueous solution of Sodium hypochlorite (5.25% w/v active chlorine) containing a few drops of a wetting agent (tween-20) with gentle shaking for 25 minutes. After pouring out sodium hypochlorite solution, the explants were rinsed with sterile double distilled water three times each for five minutes to remove all the trace of the sterilant. Subsequently, shoot tip explants having 1.5 cm long size were cultured in test tube containing 20 ml of sterilized and agar (4.5 g/l) solidified MS medium [11] fortified with BAP, Kinetin and NAA (0.5 mg/l each) plant growth regulators [12] and 20 g/l sucrose as carbon source. The pH of the medium was adjusted to 5.8 followed by autoclaving at 121°C at 105 Kpa pressure for 20 minutes. Cultures were incubated in growth room at temperature of 25°C ± 2°C under 16 hours photoperiod with photo flux density of 30 μmol m2/ s provided by cool white fluorescent light and 70% to 80% relative humidity.
In vitro rooting
For rooting studies, the regenerated microshoots were further multiplied on liquid MS medium containing 2 mg/l BAP + 0.5 mg/l Kinetin (N52) and 1.5 mg/l BAP and 0.5 mg/l Kinetin (N53) [13]. Then shoots were maintained on plant growth regulators (PGRs) free MS medium with 2 g/l activated charcoal for two weeks before transferring to a liquid rooting media in order to avoid the carry over effect of hormones from the multiplication media on rooting. Individual microshoot comprising uniform shoot length (4 cm) derived from the multiplication experiment was used for the study (Figure 1a and 1b). In this experiment, the rooting response of in vitro regenerated shoots was determined on half strength liquid medium supplemented with different concentrations of NAA (0, 3, 5 and 7 mg/l) and sucrose (0, 40, 50, 60 and 70 g/l) without agar. The pH of the medium was adjusted to 5.8 prior to autoclaving at 121°C and 1.05 kg/cm3 for 20 minutes. For each treatment 3 test tubes, each with two shoots supported by filter paper bridge (whatman filter paper) were lined up randomly in CRD with four replications. All shoots were incubated on rooting medium for four weeks in growth room adjusted at temperature of 25°C ± 2°C under 16 hours photoperiod with photo flux density of 30 μmolm2/s provided by cool white fluorescent light and 70-80% relative humidity. After four weeks of culture growth, data were recorded on number of roots per shoot, length of roots (cm) and number of rooted shoots.
In vitro rooted plantlets were taken out of the culture test tubes and thoroughly washed with water to remove all the traces of the rooting medium. After trimming out excess leaves and roots, the plantlets were transferred to trays containing autoclaved mixture of river sand, forest soil and well decomposed farmyard manure in different proportion or ratio i.e., 1:1:0, 1:1:1, 1:2:1, 2:1:1, 1:1:2 and 1:2:0. Before planting, the trays filled with the soil mixtures were properly irrigated with water. For acclimatization, the plantlets were maintained in the greenhouse for 4 weeks at about 25°C to 30°C under high humidity (>85%) by covering the trays with transparent polyethylene sheets and red cheese cloth and then sprayed with water intermittently. The cover was removed from the tray after 10 days. Starting from the 1st week of acclimatization, numbers of surviving and dead plantlets were recorded on weekly basis until the 4th week of acclimatization. Data on survival percentage was collected after 30 days. The acclimatized plantlets were further transferred to medium polyethylene bags (15 cm × 20 cm) containing forest soil and river sand in 1:1 ratio and allowed to grow in the green house for further hardening for 30 days. The experiment was carried out in five replications with 50 explants for each treatment in a completely randomized design (CRD).
Data analysis
The analysis of variance for different variables of the two experiments was performed by SAS version 9.2 [14] and for significantly different treatments, mean separation was done with REGWQ (Ryan-Einot- Gabriel-Welsch) at or below the probability level of 0.05.
Effect of NAA and sucrose on in vitro rooting of regenerated shoots
Analysis of variance showed that the interaction effects of the three factors: Genotype*NAA*Sucrose is very highly significant on root length and number of roots per shoot while rooting percentage is affected by the interaction of Genotype* NAA (Table 1). Similarly, the analysis of variance (ANOVA) showed that the main effects of NAA and sucrose; interaction effect of genotype and NAA, and the interaction effect of NAA and sucrose were statistically highly significant (p<0.0001) on rooting frequency of sugarcane micro-shoots. In both genotypes, no rooting response was observed on half strength liquid MS medium devoid of NAA and sucrose (control) (Table 2).
|Source of variation
|Root length (cm)
|Number of roots per shoot
|16.40 ***
*** = very highly significant at P ≤ 0.0001, ** = highly significant at P ≤ 0.01, * = significant at P ≤ 0.05, NS = Non-significant at P ≥ 0.05, DF = Degree of freedom, NAA = α-naphthalene acetic acid, MS = mean square, CV = Coefficient of variation
Table 1: Effect of NAA and sucrose on in vitro rooting.
Again, there was no rooting of micro-shoot recorded on half strength liquid MS medium supplemented with different level of NAA (3, 5 and 7 mg/l) without the presence of sucrose in both genotypes (Table 2). These results indicated that significance of sucrose as a source of energy in rooting medium for sugarcane micro-shoot root induction. On the contrary, root induction occurred in both genotypes when the micro-shoots were cultured in half strength liquid MS medium supplemented with various concentrations of sucrose without NAA (Table 2). This result showed that the two genotypes are rich in endogenous auxins that at least enable them to induce root. Both genotypes showed significant increase in rooting frequency because of the increase in the concentration of sucrose from 0.0 mg/l to 40 mg/l in every level of NAA. There was significant level of reduction of rooting frequency from 100% to 87.84% as the concentration of NAA and sucrose increased from 3 to 7 mg/l and 50 to 70 g/l, respectively (Table 2). This might be due to inhibition of rooting at higher concentration of NAA. Higher concentrations of NAA reduce root induction as it promotes the biosynthesis of ethylene which has inhibitory effect in sugarcane rooting [15,16] had also reported that higher concentration of NAA (7 mg/l) and sucrose (70 g/l) inhibit root induction frequency.
|Rooting percentage
|Root length
|Number of root per shoot
|Rooting percentage
|Root length
|Number of root per shoot
|0.00e ± 0.00
|0.00o ± 0.00
|0.00p ± 0.00
|0.00e ± 0.00
|0.00o ± 0.00
|0.00p ± 0.00
|83.31d ± 8.90
|4.24cd ± 0.17
|6.50mn ± 0.58
|83.31d ± 8.90
|3.45h ± 0.31
|4.00o ± 0.23
|85.41cd ± 13.90
|4.36b-d ± 0.13
|6.25mn ± 0.50
|85.41cd ± 13.90
|4.36b-d ± 0.24
|5.45no ± 0.17
|89.56a-d ± 8.64
|4.91a ± 0.07
|5.75n ± 0.96
|89.56a-d ± 8.64
|4.49bc ± 0.08
|8.28l ± 0.10
|83.31d ± 8.90
|4.64ab ± 0.16
|5.75n ± 0.50
|83.31d ± 8.90
|3.38h ± 0.21
|7.45lm ± 0.24
|0.00e ± 0.00
|0.00o ± 0.00
|0.00p ± 0.00
|0.00e ± 0.00
|0.00o ± 0.00
|0.00p ± 0.00
|100a ± 0.00
|3.81fg ± 0.39
|16.25gh ± 0.96
|100a ± 0.00
|1.52m ± 0.10
|11.35k ± 1.22
|100a ± 0.00
|4.95a ± 0.06
|23.50 a ± 1.29
|100a ± 0.00
|1.68m ± 0.03
|16.43f-h ± 0.39
|93.74a-d ± 8.64
|4.14de ± 0.21
|17.25e-g ± 0.96
|93.74a-d ± 8.64
|2.74jk ± 0.20
|18.05d-f ± 0.97
|89.56a-d ± 8.64
|3.55gh ± 0.19
|15.25hi ± 0.96
|89.56a-d ± 8.64
|1.82m ± 0.14
|14.13ij ± 0.75
|0.00e ± 0.00
|0.00o ± 0.00
|0.00p ± 0.00
|0.00e ± 0.00
|0.00 ± 0.00
|0.00p ± 0.00
|97.91ab ± 5.90
|3.91ef ± 0.14
|16.25gh ± 0.96
|97.91ab ± 5.90
|3.77fg ± 0.05
|19.28cd ± 0.69
|100a ± 0.00
|4.58bc ± 0.13
|18.00d-f ± 0.82
|100a ± 0.00
|4.54 bc ± 0.06
|21.76b ± 0.57
|93.74a-d ± 8.64
|2.90j ± 0.08
|22.75ab ± 0.50
|93.74a-d ± 8.64
|2.28l ± 0.05
|13.95ij ± 0.58
|91.65a-d ± 8.93
|2.78jk ± 0.05
|18.25d-e ± 0.50
|91.65a-d ± 8.93
|1.77m ± 0.04
|13.48j ± 0.34
|0.00e ± 0.00
|0.00o ± 0.00
|0.00p ± 0.00
|0.00e ± 0.00
|0.00o ± 0.00
|0.00p ± 0.00
|95.83a-c ± 7.73
|3.26hi ± 0.09
|18.25de ± 0.96
|95.83a-c ± 7.73
|1.04n ± 0.14
|12.73kj ± 0.73
|95.83a-c ± 7.73
|3.05ij ± 0.13
|18.50c-e ± 1.00
|95.83a-c ± 7.73
|2.34l ± 0.07
|15.43hi ± 0.57
|95.83a-c ± 7.73
|2.75kj ± 0.13
|19.00c-d ± 1.41
|95.83a-c ± 7.73
|1.73m ± 0.14
|20.02c ± 0.96
|87.48b-d ± 7.73
|2.55kl ± 0.06
|16.10gh ± 0.60
|87.48b-d ± 7.73
|1.62m ± 0.13
|19.05cd ± 0.30
*Values in the same column and with different letters(s) are significantly different from each other according to REGWQ at P<0.05
Table 2: Effect of genotype, NAA and sucrose on rooting percentage, number of roots per shoot and root length.
ANOVA also showed highly significant (p<0.0001) effect of all main and interaction effect of genotype, NAA and sucrose on root length and number of roots per shoot of the two sugarcane genotypes studied. No root was initiated on 1/2 MS liquid medium that lack NAA and sucrose in both genotypes, N52 and N53 (Table 2). It was observed that there was differential response of the two genotypes used. N52 was responsive to lower concentration of NAA than N53. Genotype N52 gave a maximum of 4.95 cm and 23.5average root length and average root number per shoots, respectively on 1/2 MS liquid medium fortified with 3 mg/l NAA and 50 g/l sucrose (Table 2 and Figure 2a) while only 1.68 cm average root length with 16.43 average roots number per shoot were recorded in N53 in the same medium composition (Table 2). On the other hand, in genotype N53, the highest of 4.54 average root length and 21.76 average roots number per shoot were produced on 1/2 MS liquid medium supplemented with 5 mg/l NAA and 50 g/l sucrose (Table 2 and Figure 2b) with this medium composition, N52 resulted in 4.58 average root length and 18.00 average roots number per shoot (Table 2).
The result also showed that an increase in the concentration of NAA from 0.0 mg/l to 3 mg/l at fixed quantity (50 g/l) of sucrose increased the average root length and roots number of N52 significantly to 4.95 cm and 23.5, respectively. In the same trend increasing the concentration of NAA from 0 mg/l to 5 mg/l maintaining the concentration of sucrose at 50 g/l increased the average root length and number of roots to 4.54 cm and 21.76, respectively in genotype N53. Conversely, further increasing the concentration of NAA and sucrose to 7 mg/l reduced the shoot length and number of root noticeably to 3.05 cm and 16.1 in N52 and to 2.34 cm and 19.05 in N53, respectively. This was due to the fact that high auxin concentration inhibits sugarcane root elongation through production of ethylene in the culture jar [15].
Similarly, increase in sucrose concentration from 0 g/l to 50 g/l along with a definite concentration of NAA (5 mg/l), significantly increased the average root length and root number to 4.58 cm and 18.00 in genotype N52, and to 4.54 cm and 21.76 in genotype N53, respectively. However, further increase in sucrose concentration to 70 g/l resulted in reduction in average root length and number of roots in both sugarcane genotypes (Table 2). Earlier reports also confirmed that higher concentrations of sucrose in the medium have a negative impact on overall rooting due to accumulation of rooting inhibitors, the reduction of rooting promoters in the medium, and the transformation of added sugars in to insoluble and storage form [16].
The current result obtained in genotype N53 was in agreement with earlier results reported by Raman et al. [17]. They obtained the longest root of 6.7 cm and the highest average roots number of 6.8 in sugarcane genotypes CoS 96268 and CoS 95255, respectively on 1/2 MS liquid medium containing 5 mg/l NAA in combination with 50 g/l sucrose; whereas NAA at 7 mg/l reduced the root length. The result obtained in N53 was also in harmony with the previous findings of Yadav et al. [18]. They reported best root growth with 5.8 and 5.7 average number of roots per shoot in sugarcane genotypes CoSe 01235 and CoS 99259, respectively Baksha et al. [19] also obtained a maximum average root length of 4.5 cm and average root number of 17 per shoot in sugarcane genotype lsd 31 on ½ MS medium with 5 mg/l of NAA.
The results recorded in the present investigation in genotype N52 was consistent with findings of Gopitha et al. [20] who obtained the highest average root length (4.9 cm) and average root number per shoot (15.1) on ½ MS liquid medium fortified with 3 mg/l NAA with 50 g/l sucrose in genotype Co671 [21] revealed a maximum of 2.50 cm average root length and 6.8 average numbers of roots at 3.0 mg/l NAA for genotype BL-4 [22] found a maximum of 13.4 average numbers of roots per shoot with 4.0 cm average root length on ½ MS medium containing 3 mg/l NAA [23] reported the highest root induction with best root growth at ½ MS medium containing 1 mg/l NAA and 60 g/l sucrose. The current result also disagrees with the report of Khan et al. [24]. They obtained a maximum roots (35) and average root length (3.05 cm) from genotype HSF-240, at 0.5 mg/l IBA with 60 g/l of sucrose. The same author also reported a maximum of 34 roots with 1.8 cm average root length from genotype CPF-237 at 1.5 mg/l IBA with 60 g/l of sucrose. However, there were other research reports of Khan et al. [24] that showed a different result, where low IBA concentration (0.5 mg/l) combined with 60 g/l sucrose gave the best results in average root number 35 per plant and root length of 3.05 cm (Figure 3).
Effect of different substrate mixture on ex vitro acclimatization of in vitro regenerated sugarcane plantlets
Statistical analysis of variance showed that the main effect of substrates mixture and interaction effect of genotype and substrates mixture were highly significant (P<0.0001) on survival rate of ex vitro acclimatized shoots (Table 3). There was a significance difference observed between the two genotypes at substrate mixture composed of sand + soil + FYM in 1:1:1 ratio (Table 4). Genotype, N52 exhibited 90% survival while genotype N53 showed only 70%. Similar trend was observed at substrate mixture of sand + soil + FYM at 1:2:1 ratio, where, N52 showed 90% survival rate whereas N53 exhibited significantly low (78%) survival rate. The highest survival rates 100% and 94%, were observed in substrates mixture of sand + soil + FYM at 1:2:0 ratio, in genotype N53 and N52, respectively; however, there was no significance difference between them. Similarly, no significance difference was observed between genotype N53 (94%) and N52 (86%) on substrate mixture made up of sand + soil + FYM at 2:1:1 ratio (Table 4). The lowest (76%) survival was obtained in substrate mixture with 1:1:2 ratio for genotype N52 while, the lowest (70%) survival rate was observed in substrate mixture with 1:1:1 ratio for genotype N53. Substrate mixtures comprised of sand + soil + FYM at 1:1:0 and 1:2:0 ratios had no significance difference in survival rate of plantlets for both genotypes. They had 94 and 92% in genotype N52 and 100 and 96% in genotype N53, respectively.
|Source of variation
|Survival rate(%)
|Substrate mixture
|Genotype*Substrate mixture
*** = very highly significant at P ≤ 0.0001, NS = Non-significant at P ≥ 0.05, DF = Degree of freedom, MS = mean square, CV = Coefficient of variation
Table 3: Effect of different substrate mixture on survival rate of ex vitro acclimatized sugarcane plantlets.
|Substrates mixture (Sand + Soil + FYM)
|Survival Rate (%)
|92ab ± 4.47
|96ab ± 5.48
|90ab ± 0.00
|70d ± 0.00
|90ab ± 0.00
|78cd ± 13.04
|86bc ± 5.48
|94ab ± 5.48
|76cd ± 5.48
|78cd ± 4.47
|94ab ± 5.48
|100a ± 0.00
FYM= farmyard manure, value in the same column with different letters are significantly different from each other according to REGWQ at P<0.05.
Table 4: The effect of different substrates mixtures on ex vitro acclimatization of in vitro generated plantlet of sugarcane.
The current result also revealed that there was no significance difference in survival rate of N53 among substrates mixtures containing different proportion of sand + soil + farmyard manure (FYM) in 1:1:0 (T1), 2:1:1(T4) and 1:2:0 (T6) ratios (Table 4). Likewise, in genotype N52, the survival rate difference was not significant among substrates mixtures composed of sand + soil + FYM in 1:1:0 (T1), 1:1:1(T2), 1:2:1(T3) and 1:2:0 (T6) ratios (Table 4). However, plantlets grown on substrate mixture devoid of FYM (T1 and T6) exhibited vigorous growth and deep green leaves while those plantlets grown on substrate mixture containing FYM (T2 and T3 in genotype N52 and T4 in genotype N53) had weak growth and yellowish leaves. This could be due to the fact that farmyard manure increases the pH of the substrate mixture to 7.4 to 7.5 [23], while the optimum substrate mixture pH value for container grown plants should range from 5.5 to 6.5 [25]. Similar result was reported by Yasmeen et al. [26].
The result obtained in the present study in genotype N53 was in agreement with previous findings of Warakagoda et al. [27] who reported 100% survival rate of sugarcane plantlets acclimatized on substrates mixtures containing sand + coir dust in 1:2 ratio [28] reported more than 90% survival when plantlets were transplanted in vermiculite. Seventeen percent plantlet survival was also reported under ex vitro condition by Baksha et al. [29]. Similarly Baksha et al. [19] found survival rate of 75% for sugarcane plantlets acclimatized on pots containing a mixture of soil + sand in 2:1 ratio. Best acclimatization response was obtained in a mixture of sand + soil + peat at 1:1:1 after three week of transplanting to greenhouse [30,31] also found 96% survival rate for sugarcane plantlets acclimatized on substrate mixture composed of FYM and soil in 2:8 ratio. Eighty five percent survival was obtained from sugarcane plantlets acclimatized on potting mixture composed of soil + sand + compost in 1:1:1 ratio [15,32] also declared 72% survival rate of micropropagated plantlet.
Half MS liquid medium fortified with 3 mg/l NAA and 50 g/l sucrose was the optimal combination for in vitro rooting of in vitro generated shoots of sugarcane genotype N52 While ½ strength MS medium supplemented with 5 mg/l NAA and 50 g/l sucrose was the best combination for in vitro rooting of shoots of sugarcane genotype N53. Substrates mixture comprising sand and soil substrate in 1:1 ratio was found to be an ideal substrate mixture for best ex vitro acclimatization with higher plantlets survival rate for both sugarcane genotype, N52 and N53.
We would like to express our gratefulness to Ethiopian Sugar Corporation for financing the study and Holetta National Agricultural Biotechnology Laboratory for provision of Tissue Culture Laboratory and all required facilities.
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HR Business Partner - EMEA
- Recruiter
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- Location
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- Salary
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- HR Business Partner
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Based in central London, the headquarters have a small group functions team and all the retail operations functions too. As HR Business Partner you'd support London and some EMEA people who are based in London. Your job is to deliver the HR strategy to your client group whilst coming up with fit for purpose initiatives and pushing back upwards on what is required to improve the HR service you can offer. The job is all about networking internally to build trust and re-enforce the wonderful culture they have cultivated.
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The SR Group (UK) Limited is acting as an Employment Agency in relation to this vacancy.
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Skier Comments & Photos from the Slopes of Cascade Mountain
What are the ski conditions like right now at Cascade Mountain? Read commentaries and see photos from fellow skiers and riders sharing their on-mountain experiences. Check back daily for new user-generated firsthand reports from Cascade Mountain to see ski conditions, new snow and general stoke from the slopes along with images. Firsthand reports are submitted via the OnTheSnow Ski & Snow Report App. Download the free app to be part of the conversation.
All languages
Snow,sunny. Making new snow. New runs and lifts.Great for Midwest!
New Snow | 80
New lifts and runs. Snow decent.
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it's great here today. No lines. Cold though. Nicely groomed.
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Cold day, lots of people & newbies on the main runs. Head to the far east runs to have snow by yourself. Those runs get shady after 3:00, so some of the more interesting terrain gets a little icy. All in all a decent day in the Midwest
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snow was nice, well groomed and soft. a couple runs started to ice up a little in the afternoon. This ski area was larger than I expected it to be, nice surprise. Will for sure be going back.
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Gallery: Cascade Mountain
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Our party marched on until we reached St. Louise de Tolouse Parish Church where we met our cousin, Nelson, his wife Sara and their daughter Kirsten.Our chance meeting at the facade of the old church brought back images of our cherished youth.
Rest of the night was spent on customary drinking session of tagayan (shot),reminiscing the good old days with childhood friends in full Lucbanin twang.As always we drank ourselves to sleep.
As evening approach, the streets burst into an array of radiance seeing that the payas (decorations) were now adorned with dazzling lights. Also, the breeze offered by sundown made the stroll more appealing and by this time the streets were nearly half-congested.
The journey back home turned out to be a trip down to memory lane with my brother's return. Im pretty sure that it will be awhile before we relive the images and experiences we shared in so short atime. Home is where the heart is so im certain that we will meet again.
photos: Fae Maaliw, Camille Barairo, Luis Miguel Maaliw


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AETC would not be able to achieve this level of success
without the help and support of our partners.
Our organization, athletes and our outreach is dependent entirely upon the financial and product support of corporations and private entities. Donate to Alpha Elite Track Club and join our community of partners.
Medical Care for Women, LLC | Pembroke Pines Woman's Center, LLC | Full Circle Healthcare, Inc | Sunday Morning Kickers of Miramar | Total Women's Healthcare of South Broward | Dr. F. Bloom | Donald F. Dixon, PA | Carlos Szajnert, PA | Stuart Rotman CPA, PA
Our partnership with Cinemacoustic began since our inception. It's both organization's desire to help athletes perform at their best through quality training and mentorship.
Quality Fabrication & Supply
National Fire Protection


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Commission Agenda Meeting July 1-2019
Notice is hereby given that the Barton County Commission will hold a meeting on the 1st day of
July, 2019 at 9:00 a.m. in the office of the County Commission, Barton County, Missouri.
The tentative agenda of this meeting will include the following open topics and any other matter that comes before the County Commission.
- Discuss correspondence received by Commission since last meeting
- Approve Minutes of the June 24, 2019 meeting
- Approve invoices to be paid on Monday, July 8, 2019
- Approve Liquor License
- Approve Monthly Reports
- Approve time sheets to be paid on Wednesday, July 3rd
- Julie Zibert, MoDot – BRO Program Agreement
- 12:00 p.m. – Depository Bid Opening
- 2:30 p.m. – Road and Bridge Crew
- Courthouse will be closed on Thursday, July 4th in observance of the Independence Day Holiday.


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happy valentines day bff images
Wellington Laboratories Inc v-day can feel like a serious struggle. offers a variety of native and mass-labelled reference standards for legacy environmental contaminants such as polychlorinated dibenzo-p continue reading; 4 comments; crafty hugs from kristina. Friend Valentine s Day eCards Send a Valentine s Day eCard to celebrate with the friends you love written by my favorite things on february 1, 2017. Whether you re serious or playful, you ll find the perfect card here posted in mft guests. Intro: Pixelated popup card today, we're so happy to welcome. I'd been scratching my head trying to come up with a card for my hubby AKA the non-romantic type this is your bonus squid row… done entirely on comic draw for ipad. I finally decided to make him this well, what do you think? font is different of course, the speech bubbles are built by the app. Want to watch this again later? Sign in to add this video to a playlist valentine's day is just around the corner – and today i'm sharing a fun printable project to add an extra dose of love (and color) to your valentine's day. Draw & COLOR at your own pace with Fun2draw APPs! Apple: i wanted to make some nudist pictures and possibly film a nudist video. Happy first of the month RRR Peeps! You know it's my day to share a project created with the new Ribbon Club Assortment i had been planning to give my boyfriend a surprise and well thought that sending him a nudist. This month it's Pink and Green Mix which happy valentine's day to all lovers & couples. Happy Valentines Day Bff ★[ HAPPY VALENTINES DAY BFF ]★ How To Get A Girl To Want You Through Text ★ Happy Valentines Day Bff ★★ Get your Ex Boyfriend Ex we share the best happy valentine images, valentine's day pictures, and valentine images with quotes. AXS let me just start by saying… why don't they make valentines day cards like these anymore? i'm in love with these vintage valentines day cards. com brings you inside access to tickets, artist news, and exclusive stories on concerts, tours, sports teams, family events, arts, theater, and festivals friendship day was originated by joyce hall, the founder of hallmark cards in 1930, intended to be 2 august and a day when people celebrated their friendships by. Whether you re celebrating Galentine s Day with your BFFs or V Day with your GF, here are the cutest gifts for the babes in your life valentine's day surprise balls. Happy Valentines Day Images – WallPapers supplies: 10 trinkets (or notes) to fill the ball crepe paper streamers, various colors crepe paper craft glue scissors a super fun valentine's day ring made with hershey's kisses and pipe cleaners. Propose Day Image with Quotes For Bae / BFF / Wife / Hubby 2017 i just had to share this adorable little craft project with you! i have always loved making valentines with maddy, owen, and cora. By valentine | February 7, 2017 i love the discussion and decision-making beforehand, i love the mess, i love the time together. 0 Comment and. Happy Valentines Day from Cool School! Crafty Carol shows you how to make your own Valentines Day party poppers perfect to make for your Valentine or as a family-owned business with over 52 years of experience, here at colt's, we offer true quality salesmanship and are here to meet your needs, whether we are. Clinique Happy is a fragrance of joy, the essence of a sunny, happy morning browse happy valentines day gif pictures, photos, images, gifs, and videos on photobucket нитки швейные, нитки купить, ножницы портновские, иглы для швейных машин we could all do with showing our appreciation more often – let your best friend know how special they are, and send them one of our best friend poems today. Wear Happy and be happy! Fresh apple, plums and bergamot mixed with the fresh-air accord this dangerous internet challenge is back, and causing kids to seriously burn and scar themselves here's a valentine's treat that is super easy, and perfect to send along with your little sweeties to share with their friends. Hey friends! Happy New Year! We are happy to announce that Tatertots & Jello and The 36th Avenue are now hosting together the new BFF LINK PARTY!! Jen and I are my daughter's love to help make. 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Reason #1232 why I don't buy the newspaper
Andrew Norton
We stopped and bought a paper today because we wanted to know what time the local Memorial Day parade was going to be. The front page of the Kalamazoo Gazette said in regards to Memorial Day Parades, "A Complete List is on B-2." Cool, I thought. When I looked at B-2 only Kalamazoo area communities had their parade times listed. What kind of a complete list is that?
So, I turned to the internet and searched - White Pigeon Memorial Day parade. The first result was ironically enough for the Kalamazoo Gazette blog at mlive.com.
I quit receiving a daily newspaper back in August of 2006. Old news and the lack of "local news" were just a couple reasons back then for not buying a newspaper.
As newspapers become obsolete they appear to have taken a hand in their own obsolescence. They put information on their websites that doesn't even appear in the physical paper.
There still is no compelling reason for me and others in the same demographic to purchase a newspaper these days.
Oh, by the way - the White Pigeon Memorial Day Parade is at 10:45 and will end at the White Pigeon Township Cemetery for a ceremony. The White Pigeon library will be open from 9am to 12pm and is showcasing an exhibit on the Civil War. Civil War reenactors will be marching in the parade and they will be available to talk to at the library.
P.S. I don't have over 1,200 reasons why I don't buy the newspaper - I was just being facetious. ;)


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James Corden thinks Alexandra had the X Factor
Gavin and Stacey funnyman James Corden has reportedly got the hots for X Factor winner Alexandra Burke. He even went to the extent of buying her a £4000 Cartier watch in an attempt to woo her.
Sources close to Alexandra have claimed that James is not the type Alexandra would normally go for, but thats not enough to deter Corden!
Its not the first time that James Corden has been linked with a high profile, sexy celebrity – within the past year he has also been linked with the likes of Leona Lewis and Alesha Dixon.


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High Desert Plant Finder & Guide
Burgundy Glow Bugleweed
Ajuga reptans 'Burgundy Glow'
Burgundy Glow Bugleweed flowers
(Photo courtesy of NetPS Plant Finder)
Burgundy Glow Bugleweed in bloom
(Photo courtesy of NetPS Plant Finder)
Plant Height: 6 inches
Flower Height: 8 inches
Spread: 12 inches
Hardiness Zone: 3
Other Names: Carpet Bugle
Short spikes of blue flowers above lovely, tri-colored foliage of cream, silver-green, and burgundy-red. Fast growing.
Ornamental Features
Burgundy Glow Bugleweed's attractive small crinkled round leaves emerge dark brown in spring, turning emerald green in color with showy creamy white variegation and tinges of purple the rest of the year. It features subtle spikes of sky blue flowers rising above the foliage from mid to late spring. The fruit is not ornamentally significant.
Landscape Attributes
Burgundy Glow Bugleweed is a dense herbaceous evergreen perennial with a ground-hugging habit of growth. Its medium texture blends into the garden, but can always be balanced by a couple of finer or coarser plants for an effective composition.
This plant will require occasional maintenance and upkeep, and should not require much pruning, except when necessary, such as to remove dieback. It is a good choice for attracting butterflies to your yard, but is not particularly attractive to deer who tend to leave it alone in favor of tastier treats. Gardeners should be aware of the following characteristic(s) that may warrant special consideration;
- Spreading
Burgundy Glow Bugleweed is recommended for the following landscape applications;
- Rock/Alpine Gardens
- Border Edging
- General Garden Use
- Groundcover
- Naturalizing And Woodland Gardens
Planting & Growing
Burgundy Glow Bugleweed will grow to be only 6 inches tall at maturity extending to 8 inches tall with the flowers, with a spread of 12 inches. Its foliage tends to remain low and dense right to the ground. It grows at a medium rate, and under ideal conditions can be expected to live for approximately 10 years.
This plant does best in partial shade to shade. It does best in average to evenly moist conditions, but will not tolerate standing water. It is not particular as to soil type or pH. It is somewhat tolerant of urban pollution. Consider applying a thick mulch around the root zone in both summer and winter to conserve soil moisture and protect it in exposed locations or colder microclimates. This is a selected variety of a species not originally from North America. It can be propagated by division; however, as a cultivated variety, be aware that it may be subject to certain restrictions or prohibitions on propagation.
Please note: Our Plant Guide is all about plants that will work in the high desert. It is not, nor is it intended to be, a current list of plants in stock on hand at each location or at our farm. We purposely do not sell or ship plants via the internet. Plant questions and availability are best determined in-store.


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$25 Bonus Cash with first deposit
How to Use Your Coupon Code
Click "Show Coupon Code" button
- This will open a new tab that takes you to dober.games
Copy the code
- Select and copy the code that is highlighted in yellow on this page (in place of the button after clicked)
Enter the code at checkout
- Paste your code in the appropriate box, like below:
Enjoy your savings
- Hopefully you saved a lot!
Previous (expired) Codes
These codes probably won't work, but you can try them if you like 🙂
Coupon code: 75FREE4REDDIT ($75 off sitewide)
Save with Dober Games Coupons
It's no secret that shopping online saves you time and money.
That's why we're always updating this page with the latest Dober Games coupon codes.
About Dober Games
Want to learn more about Dober Games?
Their about page is probably a good place to start.
You can also contact them here.
Have a question? Maybe the FAQ can help.
Or check out their socials at Facebook, Twitter and YouTube.
Dober Games is located in Wilmington, Delaware.


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Paul & Tammy Huff's start-up tale reads like a dream for many new child care center owners. The first year they opened, they had 150 children on their first day. Paul says that, "there were so many people there that my dad was giving group tours." That's the kind of opening most of us can dream about.
However, running a smooth child care is more than just opening doors and enrolling children. Paul remembers coming in on their first day at 8:30 am and his wife Tammy, was in the bathroom crying, with forms lying around everywhere. They had not thought to ask parents to bring in registration forms before their first day, so they had over 100 parents trying to meet teachers, drop off forms, and get to work all at the same time. Paul recalls that "it was pure chaos."
Still, with some hard work and no small amount of luck, they made it through. Within 2 weeks, they were full with 300 hundred children. Five years later, they opened a second school and that one started off doing well, too.
Building a Foundation
Not all stories are like Paul's. In fact, most aren't. For most of us, filling our building takes loads of sweat, tears, and long days. Clearly there was a very high demand in Paul's area, but they also realized on day one that having systems and procedures in place is key.
When most new businesses start out, they are the least likely to invest in support, but this is often the best time to do so. Having a strong foundation will not only set you up for long-term success, it will allow you to focus on running your business, supporting teachers, and communicating with families, rather than the busy work of creating email templates or newsletter templates.
Don't Re-invent the Wheel
Hiring someone to help you and working with other directors that have already been through what you are now going through will also save you inordinate amounts of time. There are thousands of child care centers throughout the US and most of them need similar documents, tools, and procedures. Why re-invent the wheel, when you can network with people who have already done the work for you and are willing to share? That's a big part of what we do here. 🙂
Cracks Begin to Show
Getting back to Paul's story, things had been going great with their two buildings always near capacity, but, by the fall of 2014, there luck had begun to run out. They had dropped to 80% enrollment in both schools and Paul decided it was time to kick it into high gear and figure out what was wrong. This is part of what prompted Paul to attend his first Child Care Success Summit. He signed up for our Diamond coaching program that year mainly because he wanted someone to come to his schools and see them with a fresh set of eyes.
Staff Training & Team Building
Something Paul repeatedly noticed upon entering his schools was that the director was always in the office with two or three other teachers and always seemed to be refereeing some kind of dispute. He also was shocked to see that every time a child came in for a tour, teachers would mutter under their breath, "Oh no, not another child." Something had to be done.
Kris came in and devised training and team-building exercises for his staff, emphasizing what the schools could do with a full center. By March, they'd enrolled an additional 80 kids and both schools were full by following the recommendations of Kris and suggestions from peers in the program. Paul was amazed, saying, "Those 80 kids mean $20,000 a week for us."
You Can't Do It All
Paul learned that building a strong foundation is the key to long-term success. Another thing he learned is that you can't do it all yourself. Trying to do everything yourself is one of the biggest mistakes many child care directors make. They're the teacher trainer, sales person, finance manager, marketer, and everything else all rolled into one. No one can do all of that and deliver quality on every level.
When Paul wanted to create a new music program for his Pre-K classes, he decided to give delegation a try and asked his son, Steven, to develop the program. Not only did Steven deliver, teachers were amazed and now he has directors from other centers clamoring to use his program.
By delegating tasks, you free yourself up to focus on the key drivers of your business, but you also allow your staff opportunities to grow and develop. Think of delegation as an investment in your team. They often become more committed and you end up with something better than if you had tried to rush it in between all your other tasks.
Excited for More
"I say we've been successful in implementing 20% of it (ideas from Kris and other members in his group) and I've still got 80% to go", says Paul. With the huge success he's seen implementing just 20%, it's going to be amazing to watch and see what he can do implementing the other 80 and we're excited to be part of that journey.


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Funsol polvo 60 g
Price €6.53
Deodorant and antiperspirant powder for feet and footwear
Peusek express deodorant is a powder made with natural ingredients such as Bamboo and Salvia that protect the feet and keep the shoes dry and odor free.
It eliminates the bad smell of the footwear, including the sneakers, as well as the closets or rooms where they are stored.
Contains no parabens, no allergens, no aluminum.
Sprinkle daily on feet and footwear.
Avoid contact with eyes or mouth.
Do not apply on irritated or injured skin.
You might also like


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Dr. Jan Bulten
Dr Jan Bulten, addressed by his patients as Dr Bertel, is an experienced General Practitioner. Dr Bertel ran his own practice in Netherlands. He has been practicing in WA since 2008 and has six years experience of working in a rural town. He believes that everyone has different experiences of disease and health management, that deserves an individual approach based on trust and respect.
In his free time, Dr Bertel is passionate about classical singing, cycling, music and tries to keep fit.
His main interests are:
- general practice
- men's health
- sexual health (insertion / removal Implanon included)
- mental health
- some minor surgery (lumps and bumps)
- child health
- refugees and migrant health
- aboriginal health


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Enjoy the best music of the moment during the 4 hours of partying animated by one of the best DJs on the island.
The Supercat exit is made from the Puerto Base in Puerto Rico, but if you stay in Playa del Inglés or Maspalomas, we offer you a pick-up point at the bus stop in front of the Playa del Ingles Aparthotel at 2:30 p.m.
The party Mtv and Pacha takes place throughout the ship. On the top floor, on the deck, you can sunbathe or dance on the dance floor, as well as contemplate the spectacular views of the Gran Canaria coast during the trip that takes us from Puerto Rico to Taurito. Observe the high cliffs, the great marine caves, the beautiful beaches and the splendid hotels. Oh! And on clear days, the Teide, the highest peak in Spain with 3718 meters, will accompany us throughout the voyage.
On the lower floor there is the dining bar, where you can enjoy a meal consisting of marinated chicken, potatoes with mojo and salad, or drink sangria, beer, wine or soft drinks. All included in the price.
The lower floor is also where the platform where you jump to bathe in the sea when we stop near the coast, and where is the yacuzzi in which you can soak during the whole party.
Departure point: Puerto Rico Base PortDay departures: Tuesdays and Fridays at 3:00 pm Duration: 4 hours ** Check departure times when making the reservation in case there is a change
Includes: open bar (beer, sangria, soft drinks, water), lunch (chicken, potatoes with mojo, lunch), live DJ, transfer from Playa del Inglés to the boat.
Transfer from Playa del Inglés: Pick-up point at the bus stop in front of Aparthotel Playa del Inglés at 2:00 pm on the days of departure.


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Looking for Spoken english tutors in Golkpark? We have experienced qualified private tutors who can help you taking classes and lessons for Spoken english. Both one to one and group class options are available near your locality or area. View the profiles of the private teachers or trainers woth their qualification, expertise, teaching techniques,rates and availability.
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say what needs to be done lead by example, and make opportunities for people to become more comfortable with one another. You may be able to inspire positive changes in them by: thesis on fashion trends Enthusiastically promoting new policies and practices that aim to adjust the company culture. However, having a sound idea of what you want the culture to be like from the very beginning will help give shape to your vision as the company leader. Never forget that while most of the time your companys culture focuses on what happens within your company, it can have a huge impact on how others see your business. By embodying your ideal company culture and hosting social events for your team, you can create an atmosphere of innovation, communication, and trust. You might even interview a person multiple times; invite other team members along for the interviews so they can offer their opinions on your candidate. Simply put, your companys culture is its personality. Courses consist of engaging, bite-sized video lessons that make concepts easy and fun to learn. Students can watch the lessons on their own schedule and transfer their credit recommendations to thousands of colleges and universities. For example, every business wants to see tangible results in the monthly balance sheets.
Ethics might come into play when youre deciding which values are the most important to your company. Making it clear that youre willing to listen to feedback about changes from everyone, even people who were naysayers from the beginning. M has been an nccrs member since October 2016. Write down what you want the culture to be, and prepare a presentation that will help you get your message across. You might decide to remodel the office by the end of the year, start hosting a monthly social gathering for the team, or begin using new collaborative software to encourage team thinking. You could also ask job candidates to take a personality test during the hiring process. In this lesson, you will discover the difference between the two, and the effect of two selves at odds. Other goals are easier to measure. Find the Right People, more and more, businesses are putting increasing weight on soft skills versus hard skills. Here is a list of some values that many companies prioritize: Respect and fairness, trust and integrity. Its like a cyber-extension of your work environment. Students can save on their education by taking the m online, self-paced courses and earn widely transferable college credit recommendations for a fraction of the cost of a traditional course.


text: Uber Air will be taking off in 2020, but it'll be a pilot project with actual pilots flying eVTOL vehicles, not a fully-automated service. Melbourne will sit alongside Dallas and Los Angeles as a pilot city for Uber Air, with trials to start in 2020. The company will work alongside Telstra, Macquarie and the owner/operator of Westfield, along with Melbourne Airport, to create the infrastructure and communications network necessary for airborne ride-sharing. The service relies on electric vertical take-off and landing vehicles (eVTOL) drones with space for four passengers and their "personal bags and backpacks". They'll be piloted by humans to start with, but Uber believes automation will eventually take care of all the driving. "Australian governments have adopted a forward-looking approach to ride-sharing and future transport technology," said Susan Anderson, regional manager for Uber in Australia. "This, coupled with Melbourne's unique demographic and geospatial factors, and culture of innovation and technology, makes Melbourne the perfect third launch city for Uber Air. We will see other Australian cities following soon after." Although details are still up in the air, Uber eventually wants to fly customers to their destination 500 or 600m above the ground at around 240km/h. It also wants rides to "cost as little as an Uber X ride" – we'd suggest the days of low-cost eVTOL travel are a long way off, though. Using those numbers, it would cost roughly $65 to fly from the CarAdvice office in Richmond to Melbourne Airport, and take just eight minutes. That's… [Read full story]


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Stunning, stately colonial in the Allenwood School district. Features: HW floors & custom moldings. Lg. Center island, gourmet EIK w/ quartz counters in an open floor plan for entertaining. Formal living/family room w/gas fireplaces, dining room w/beautiful butlers pantry. 4+ bedrooms, 4 full/ 2 half baths (walk-in closets). Huge 1st floor master suite with marble bath & laundry. Grand 2 story foyer leads to 3 lg bedrooms, jack-n-jill bed/bath. Addl laundry room on this level. Lavish finished basement, possible in-law suite with separate entrance, 9ft ceilings, custom upgrades. 4 zone HVAC, owned transferable solar panels, providing income producing annual rebates & no electric bills. Beautiful deck off expansive yard, 3 car garage, extensive alarm and security camera system. Must see!
Listing courtesy of Continental Real Estate Group.


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Practically this entire week has been all about our adventure at the county fire. Between the memories we've made and the super fantastic purchase I made (part 1 and part 2), the only thing I haven't shared is the food we devoured. Well that's what I'm here to share with you all right now!
The Del Mar Fair is infamous for it's wide variety of deep fried foods, the carnival rides that light up the night sky, and the vendors that fill each hall with tons of goodies galore. While we didn't do any rides this year - my padawan opted to play the games instead, to which he won 3 decent prizes - we did venture around the vendors (which I already discussed) and ate loads of decadent eats.
You may remember last year when I shared what as on the menu at that time, but this won't be nearly as adventurous as it was...unfortunately.
While I don't think I'll ever stop going to the county fair, I do think that every year their food items get less and less interesting. This doesn't mean it's not delicious, it's just not as "oh man I have to try this!" shocking. So there aren't too many pictures, but I will say this: I forgot one.
I know, I know, shame on me. Trust me it's nipping my behind as I type this.When I got home from the fair that day, I had a mini panic attack, because I had realized my devastating mistake. It's a heartbreaking reality, but it is what it is.
First up is the Sriracha corn dog.
I would've sworn I had talked about this last year, but apparently I had not. This corn dog is my favorite corn dog of all time. The hot dog inside seems to have been infused with Sriracha or simply spent time soaking it in. The outer breading isn't spicy, but there was an abundance of breading overall. Semi-disappointed, but extremely spicy nonetheless. The overall thick hot dog was a wonderful snack while I enjoyed some wrestling.
My little padawan decided to go for a corn dog as well, but less on the spicy side. He chose Hot Dog on a Stick - a classic favorite of his - and got his own hot dog and lemonade.
My all-time favorite eat of the day was by FAR the turkey tacos I found at Juicy's.
Another thing our San Diego county fair is infamous for, is their giant turkey legs. Some of the juiciest, tender turkey meat you'll ever sink your teeth into. Something I can't even begin to describe in words except for "heaven". Well this year Juicy's had turkey tacos made from these very turkey legs.
They came with beans and salsa fresca, all on top of corn tacos. It didn't have salsa - which it could've used - but that honestly didn't affect the taste of these beauties and that's saying something! No guacample either, but still beyond amazing. I was so incredibly pleased with this find, scarfing down everything within minutes, making the fact that I forgot to snap a pic that much more painful.
Trust me I'm upset with myself, but just know that if you ever get a chance to try the Juicy's turkey tacos (as they do travel with the county fairs - not sure which ones though), do it! You won't regret it and if you love their turkey legs, you'll be down right in love!
Happy Friday Jedi's! I hope you have a splendid weekend filled with love, memories, and true happiness. Remember to keep on rockin' and remember, you are perfect!


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Sun protection campaigns should natu
The biological half-lives of chloramphenicol ranged from 26 minutes in the pigeon, Columbia livia, to 288.3 minutes in the bald eagle, Haliaeetus leucocephalus. The APDA augmentin ulotka system was well received by students and was shown to offer improvements in data administration (increased portability, time to completion) and reduced missing data. The small intestine was affected 3 times, the colon once, One patient died after resection of the affected bowel segment.
Gene flow between the different geographical populations and genetic differentiation within side effects of augmentin each geographical population were also detected. trans-Resveratrol (t-Res) is a natural phenolic compound with various biological activities such as antioxidant, anticancer, cancer chemopreventive, cardioprotective, and neuroprotective.
Typical MRI changes noted with vigabatrin were noted augmentin for uti in three controls. Finally, recommendations are offered for operating an evidence-based drug court. Dependence of arsenic fate and transport on biogeochemical heterogeneity arising from the physical structure of soils and sediments.
Of a total of 1316 studies identified from database searches, 34 were included in this systematic review. This paper addresses the problem of detecting salient areas within natural images.
In a previous study on 30 patients allergic to kiwi, we identified a major allergen of 30 kd against which all sera tested clearly reacted. Chlorophyll fluorescence measurements revealed that non-photochemical quenching increased, whereas the electron transport augmentin vidal rate decreased under salt stress. To unlock the greatest value, a more enterprise-wide strategic approach to healthcare IT is needed.
Is the rise of the action potential of the ranvier node controlled by a paranodal organ? Results showed that 118 out of 168 indoor seasonal mean values were higher than the corresponding outdoor concentrations. When used appropriately, sunscreen decreases the amount of ultraviolet (UV) radiation exposure to the skin and is recommended to prevent augmentin side effects skin cancer.
In this study oral treatment with methylprednisolone was as effective as intravenous treatment in acute relapse of MS. Pulmonary complications after allogeneic hematopoietic stem cell transplantation: pathogenesis and therapeutic applications augmentin torrino for bronchiolitis obliterans
The intensity of posttreatment surveillance recommended by ASPRS members caring for patients with cutaneous melanoma varies markedly. Mouse Mx2 protein inhibits vesicular side effects of taking augmentin stomatitis virus but not influenza virus. Various prognostic factors, including age, surgical procedure, T classification, N classification, histological grade, and status of margins were assessed in univariate and multivariate analyses.
Protein immobilization using the pristine and the cured PGMA NFs could be considered augmentine as an outstanding protocol for facile protein delivery. Managing bone mineral density with oral bisphosphonate therapy in women with breast cancer receiving adjuvant aromatase inhibition. Stress potentiates decision biases: A stress induced deliberation-to-intuition (SIDI) model.
The therapy resulted in lesser tumor incidence, significant inhibition of tumor growth and enhanced percent survival in treated interactions for augmentin animals as compared to untreated controls. African Americans, especially those in the South, suffer a disproportionate burden of obesity and are at high risk for perceived discrimination (PD).
In the batch culture, Bcl-x(L) overexpression suppressed apoptosis, as evidenced by a decreased amount of cleaved caspase-7 and PARP. The maximum entropy method was also employed to clarify the lithium distribution in these materials.
High fever occurs in these conditions, frequently before the skin and/or mucosa is affected. GNSA was unable to support the proliferation of any Gram-positive organism or yeast/fungi, but was successful in supporting the growth of all Gram-negative organisms challenged. Response to starvation before and after a jejuno-ileal bypass operation for morbid obesity.
Effects of modafinil and R-modafinil on brain stimulation reward thresholds: implications for their use in the treatment of psychostimulant dependence. Several mutlticentre efforts are already under way to meet these needs. The reaction with planar Ag thin films produces alkanethiol self-assembled monolayers (SAMs), whereas the reaction with Ag islands yields two dissimilar products depending on the size of the islands.
To recognize gestures regardless of motion speed, the proposed method utilizes the keyframe extraction method. Alterations in renal prostaglandins (PGs) may contribute to some of the renal manifestations in diabetes leading to nephropathy.
This gene codes for the common subunit of the leukocyte integrins LFA-1, Mac 1, and p150,95. Thyrotoxicosis associated with augmentin in pregnancy the use of amiodarone: the utility of ultrasound in patient management.
Should family physicians routinely screen augmentine 875/125 patients for cognitive impairment? WS diagnosis is often delayed since misdiagnosed as autoimmune diabetes. Moreover, the available evidence shows that the human auditory nerve follows this same general organizational plan.
The p53-MDM2 feedback loop is vital for cell growth control and is subjected to multiple regulations in response to various stress signals. Based on these findings, alpha-iso-cubebene is proposed as an effective new anti-inflammatory agent that may side effects for augmentin have a potential therapeutic use for the prevention and treatment of vascular diseases.
An intravenous injection of physostigmine did not prolong survival and had no favourable effect on the cardiotoxicity caused by orphenadrine and imipramine. Characterization of a new set of mutants deficient in fermentation-induced loss of stress resistance for use in frozen dough applications.
Dynamics and spin relaxation of excitons in GaAs/AlxGa1-xAs quantum wells. Interactions between VTA orexin and glutamate in cue-induced reinstatement of what is augmentin cocaine seeking in rats.
The modified alphaL-crystallin was examined by SDS-PAGE and what is augmentin used for fluorescence measurements. Disappointingly low rates of pathologic complete remission have been noted in several phase II studies. Iatrogenic injuries to the vascular system are a rare but serious complication of hip surgery.
Patients diagnosed with BPPV between January 1, 2007 and December 31, 2012 were recruited from the National Health Insurance Research Database in Taiwan. The signal responsible for nucleolar localization is encoded by the amino acid residues 87 to 92, RLSRKR, located near the C terminus of the ribosomal protein. Cloning and spatiotemporal expression of the follicle-stimulating hormone beta subunit complementary DNA in the African catfish (Clarias gariepinus).
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Melbourne Elevator Modernisations is an Australian owned Melbourne based Company specialising in the upgrading of existing elevator systems. With over 30 years of Lift industry experience covering all facets of the industry from Elevator Design, Manufacturing and Installation through to Technical support and Project Management we are able to cater for all our customers' needs in house as a one stop shop no matter how complex or simple the requirements are.
At Melbourne Elevator Modernisations we pride ourselves on offering honest advice regarding the upgrade possibilities and options available to our customers with realistic time frames and costs for all work we quote.The Company Owner Scott Veal has been able to assemble a modernisation team with necessary skills, hands on experience and exceptional can do attitude to be able to take on all types of Modernisation projects in the low to medium rise lift market from simple service lift upgrades through to complete multi lift upgrades up to 30 floors involving complex designs and tight time frames.
Put simply, Upgrading Elevator Systems is what we do.
Feel free to contact our team for information and advice about modernising your older lift.
|Managing Director:
|Andrew Lewis
|Installation Manager:
|Scott Veal