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For influenza viruses, pyrosequencing has been successfully applied to the high-throughput detection of resistance markers in genes encoding the drug-targeted M2 protein and neuraminidase. In this study, we expanded the utility of this assay to the detection of multiple receptor binding variants of the hemagglutinin protein of influenza viruses directly in clinical specimens. Specifically, a customized pyrosequencing protocol that permits detection of virus variants with the D, G, N, or E amino acid at position 222 in the hemagglutinin of the 2009 pandemic influenza A (H1N1) virus was developed. This customized pyrosequencing protocol was applied to the analysis of 241 clinical specimens. The use of the optimized nucleotide dispensation order allowed detection of mixtures of variants in 10 samples (4.1%) which the standard cyclic nucleotide dispensation protocol failed to detect. The optimized pyrosequencing protocol is expected to provide a more accurate tool in the analysis of virus variant composition.
The influenza virus is an enveloped single-stranded negative-sense RNA virus that is an important respiratory pathogen which causes annual epidemics and occasional pandemics (27). As a result of a high mutation rate of viral RNA polymerase (4, 19), the influenza virus exists as a quasispecies, a complex mixture of closely related genomes. When selective pressure is applied, variants carrying favorable changes gain growth advantage and establish dominance (16). Influenza virus hemagglutinin (HA) is responsible for binding to cellular receptors and for membrane fusion during the virus entry into the host cell (23). Mutations in HA can allow the virus to adapt to a new host and evade the human host immune system (25) and can be responsible for altered receptor specificity, virulence, and other traits. Therefore, the ability to detect HA variants, even when they are present at a low frequency within the virus population, is desirable.
Currently, there are several assays that allow the monitoring of mutations at specific nucleotides. Sanger sequencing is considered the "gold standard" for analysis of virus genome variations (26). However, the interpretation of sequencing chromatograms can be difficult for mixed-genome variants containing insertions, deletions, or multiple nucleotide substitutions (23). Sanger sequencing (31), high-resolution melting curve (HRM) analysis (14), real-time quantitative reverse transcriptase PCR (qRT-PCR) (2), rolling cycle amplification (RCA) (28), single-strand confirmation polymorphism (SSCP) (10), resequencing array (29), and, recently, pyrosequencing (3, 7, 18, 20) have been employed for the analysis of specific mutations in genomes of different viruses.
Pyrosequencing technology is based on luminometric detection of the light signal produced by luciferase (21). The reliability and flexibility in assay development have made pyrosequencing a widely used method for various screening and diagnostic applications (13). Pyrosequencing platforms have been useful for high-throughput sample screening for molecular markers associated with drug resistance or other traits (3, 7, 12). Recently, pyrosequencing was used for the timely detection of resistance to adamantanes and oseltamivir during the 2009 influenza A virus (H1N1) pandemic (pH1N1) (6).
Although pyrosequencing is efficient and well suited for the analysis of short sequences, the technology is still fairly new and there are challenges for certain applications. For example, it was shown for the KRAS oncogene that the presence of a mixture containing more than two major genomic variants can complicate pyrogram interpretation (26). Such mixtures can also cause inaccurate identification of single nucleotide polymorphisms (SNPs).
Identification of molecular markers of virulence in influenza viruses remains a challenging task. The emergence and rapid global spread of a novel pH1N1 influenza virus in 2009 highlighted the need for high-throughput analysis to detect new variants of this rapidly evolving pathogen. Overall, the pH1N1 virus caused mild illness in most cases; however, numerous severe and fatal cases were also reported (30). Analysis of the HA gene of the 2009 pH1N1 virus revealed several mutations (Table 1) at the aspartic acid (D) residue at position 222 (225 in H3 numbering). An association of clinically severe cases with the D222G substitution (GAT → GGT) in the HA1 subunit was reported (11), although no such association was found in other studies (1). Also, a considerable frequency of N222 variants (GAT → AAT) was found among severe and fatal cases (11, 17). Noteworthy, changes at D222 have been associated with adaptation of a seasonal, nonpandemic human H1N1 influenza A virus to a new environment (e.g., adaptation to mice and embryonated chicken eggs) because this amino acid is a part of the receptor binding site (8, 9, 24). To further investigate the potential role of amino acid substitutions at position 222, it was highly desirable to develop an assay which would allow accurate, high-throughput detection of multiple variants within a clinical specimen. In this report, we describe the use of a customized dispensation order of nucleotides for pyrosequencing that enables the accurate detection of HA variants in pH1N1 viruses.
Virus isolates and clinical specimens.Seven pandemic A (H1N1) influenza virus isolates and 241 clinical specimens (throat swabs, nasal swabs and washes, nasopharyngeal swabs, and sputum specimens) submitted to the Centers for Disease Control and Prevention (CDC) between November 2009 and January 2010 as part of the U.S. virus surveillance activity were used for this study.
Primer design.To detect mutations at the 222 position of the HA, three primers were designed with the use of PSQ Assay Design software, version 1.0.6 (Qiagen, Valencia, CA). The primers SW-HA-F696 (5′-CAAGAAGTTCAAGCCGGAAATAGC-3′, forward primer) and SW-HA-R799b (biotin-5′-ATTGCGAATGCATATCTCGGTAC-3′, reverse biotinylated primer) were used to amplify DNA fragments by RT-PCR. The primer SW-HA-F715 (5′-CAATAAGACCCAAAGTGAGG-3′, sequencing primer) was applied for detecting the target region.
RNA extraction and RT-PCR.Viral RNA was extracted from 100 μl of sample using either a MagNA Pure Compact or MagNA Pure LC 2.0 nucleic acid isolation system (Roche Diagnostics, Basel, Switzerland). RNA was eluted in 50 μl and stored at −30°C. A SuperScript III one-step RT-PCR system with Platinum Taq High Fidelity enzyme (Invitrogen, Carlsbad, CA) was used for cDNA synthesis and amplification as described by the manufacturer. Briefly, the reaction was initiated at 50°C for 30 min. Then, Taq DNA polymerase was heat activated at 94°C for 2 min. This was followed by 45 cycles at 94°C for 15 s, 50°C for 30 s, and 68°C for 1 min. The final elongation time was 5 min. Primers SW-HA-F696 and SW-HA-R799-b were used for RT-PCR at a final concentration of 0.4 μM. The amplified RT-PCR products were visualized by electrophoresis on 2% agarose E-gels (Invitrogen, Carlsbad, CA).
Sequencing by dideoxy chain termination method (Sanger sequencing).Primers designed for the RT-PCR step of pyrosequencing (SW-HA-F696 and nonbiotinylated SW-HA-R799) were used to amplify the cDNA fragment for Sanger sequencing. Amplified RT-PCR products were purified using ExoSAP-IT reagent (USB, Cleveland, OH). Sequence template was synthesized with an ABI Prism BigDye Terminator kit (Applied Biosystems, Foster City, CA). Products of sequencing reactions were treated with XTerminator solution (Applied Biosystems, Foster City, CA), according to the manufacturer's protocol. Sequences generated in an ABI Prism 3730 genetic analyzer (Applied Biosystems, Foster City, CA) were analyzed using Lasergene software, version 7.0 (DNAStar, Madison, WI).
Pyrosequencing.Pyrosequencing reactions were performed on a PyroMark Q96 ID instrument as described by the manufacturer (Qiagen, Valencia, CA). The SW-HA-F715 sequencing primer was used for all reactions at a final concentration of 0.45 μM. Both the sequence analysis (SQA) and the SNP modes of the PyroMark Q96 ID instrument were utilized for analysis of substitutions at the 222 position in the HA. Two different nucleotide dispensation orders were used for pyrosequencing in SQA mode: the cyclic dispensation order (GATC)6 and the customized dispensation order ATGTAT(CAGT)6 (see Results). SNP analysis was conducted to determine the percentage of each variant within a mixture containing two virus variants. These percentages were calculated by the PyroMark ID software. The dispensation order for SNP analysis was generated by PyroMark ID software on the basis of the target sequence and the site for SNP analysis. For a mixture containing GAT and AAT variants, the generated dispensation was CGAGTCAGA.
Population cloning.To confirm the presence of virus variants within mixtures, cloning of RT-PCR products amplified with primers SW-HA-F696 and nonbiotinylated SW-HA-R799 was performed using a TOPO TA cloning kit as described by the manufacturer (Invitrogen, Carlsbad, CA). One hundred individual bacterial colonies containing recombinant plasmids carrying an individual RT-PCR fragment were randomly picked and analyzed by pyrosequencing of the PCR product amplified with primer SW-HA-F696 and biotinylated primer SW-HA-R799b. Pyrosequencing analysis was performed as described above.
Statistical analysis.t-test analysis was used to determine whether there was a statistically significant difference between the percentage calculated for virus variants in a mixture tested with the customized nucleotide dispensation order in SQA mode or in SNP mode. Statistical significance was set at a P value of <0.05. All probabilities were two-tailed.
Pandemic H1N1 influenza viruses with changes at each nucleotide of the triplet for amino acid 222 of the HA protein were detected by Sanger sequencing. Specifically, a single nucleotide substitution at the first (GAT → AAT), the second (GAT → GGT), or the third (GAT → GAA) position (Fig. 1A to D) was seen. In addition, sequencing showed that some viruses contained a mixture of variants (Fig. 1E to G). For example, the chromatogram in Fig. 1E showed a polymorphism for the first nucleotide (G/A), which suggests the presence of two variants: GAT and AAT. Another virus had two polymorphisms, at the first and second positions (Fig. 1F and G), which might reflect the presence of up to four variants: GAT, AAT, GGT, and AGT, encoding aspartic acid (D), asparagine (N), glycine (G), and serine (S), respectively. However, the actual variants cannot be reliably ascertained on the basis of Sanger sequencing. Therefore, isolates (n = 7) with an individual GAT, AAT, GGT, or GAA variant as well as mixtures were selected to be analyzed using the pyrosequencing assay.
Analysis of virus isolates using the pyrosequencing assay (cyclic nucleotide dispensation).First, the viruses were analyzed in the pyrosequencing assay using a standard cyclic dispensation order, (GATC)6, in SQA mode. With this approach, the single variants were readily identified (Fig. 1A to D, cyclic dispensation). However, results for the samples containing mixtures of variants were not in agreement with the sequences determined by the Sanger method. For example, a mixture of two variants, GAT and AAT, was interpreted as a single wild-type variant, GAT, by the PyroMark ID software analysis (Fig. 1E). Visual inspection of the corresponding pyrogram (Fig. 1E) suggested the presence of the additional variant (AAT), on the basis of the increased height of the first A peak in relation to the first G and T peaks.
In another instance, Sanger sequencing indicated the presence of GAT and GGT variants, while the PyroMark ID software still identified only the wild-type variant, GAT (Fig. 1F). Contrarily, the increased height of the first G peak in comparison to the height of the first A peak in the pyrogram suggested that in addition to the wild type, the variant GGT was present. However, in another sample (Fig. 1G), even close inspection of pyrograms did not provide any evidence for the presence of additional variants detected using the Sanger method.
Target-specific customization of nucleotide dispensation.Since pyrosequencing using the standard cyclic nucleotide dispensation was unable to accurately determine the variants in the mixtures, a customized dispensation order was designed with the intention to improve the resolution of pyrograms. Because all three variants of interest (GAT, GGT, and AAT) share a T in the third position, we proposed a new customized order: ATGTAT(CAGT)6. Consequently, when the first A nucleotide was dispensed, both A nucleotides for an AAT variant were incorporated. The subsequently dispensed T would then be incorporated as the T of an AAT variant. Therefore, the peak height for the A nucleotide would be twice that of the T peak and the T peak would reflect the total portion of the AAT virus variant in the mixture. The next dispensation of a G would be incorporated as the first nucleotide of a GAT variant or the first and second nucleotides of a GGT variant. The presence of the T peak for the T nucleotide dispensed after the G would confirm the presence of a GGT variant. In this case, the height of the T peak was used to calculate the final proportion of the GGT variant. The following A nucleotide dispensation would extend a GAT variant, and the final T dispensed prior to the cyclic (CAGT)6 portion of the dispensation order would indicate the last peak for a GAT variant. This T could also be used to analyze the proportion of a GAT variant present in a mixture. Thus, the proposed order of dispensation allowed separation of each of the three variants on the basis of a location of the T nucleotide on a pyrogram.
For a theoretical sample containing equal amounts of three variants (AAT, GGT, and GAT), a T nucleotide at the second, fourth, or sixth position would indicate the presence of all three variants, respectively (Fig. 2B). Using the standard cyclic order of nucleotide dispensation, this theoretical sample would be incorrectly identified to have the GAT variant solely present in the sample, as depicted in Fig. 2A.
Since the respective peak heights in a pyrogram were proportional to the amount of incorporated nucleotide, the customized dispensation proposed here allowed the quantification of each of the three variants in a mixture based on the analysis of T peak heights. The following algorithm was derived to calculate the proportion of each variant: (Tn − TBkg)/(T2 + T4 + T6 − 3 × TBkg) × 100% = SNPn, where Tn (n = 2, 4, or 6) is the peak height of the T nucleotide dispensed at positions 2, 4, and 6 and Bkg is the average background of T calculated from the peak heights of the T nucleotides dispensed at positions 10, 14, and 18 where no T residues exist in the sequence.
Comparison of customized nucleotide dispensation order, SNP analysis, and cloning analysis.The next step was to compare the results using customized nucleotide dispensation order, SNP analysis, and cloning analysis for the same specimen. A virus isolate which contained two virus variants, GAT and AAT, was used. The pyrogram generated with the customized nucleotide dispensation order clearly showed the presence of both variants (Fig. 3).
Using the customized nucleotide dispensation, the proportion of the sample with the GAT and AAT variants was calculated using the peak height data described in the algorithm (Table 2) and was found to contain 86.8% ± 0.2% of the GAT variant and 13.2% ± 0.2% of the AAT variant. The analysis of this specimen in SNP mode showed that GAT and AAT variants were present in proportions of 87.7% ± 0.1% and 12.3% ± 0.1%, respectively. The similarity of the data obtained by the two approaches was statistically significant (P < 0.02) (Table 2).
For the same specimen, DNA cloning showed the proportions of GAT and AAT variants to be 84% and 16%, respectively (Table 2). Thus, the customized nucleotide dispensation order for pyrosequencing could accurately identify the proportions of the virus variants in mixtures.
High-throughput analysis of clinical specimens using pyrosequencing.To assess the ability of the customized pyrosequencing assay to detect variants at position 222, 241 clinical specimens positive for pH1N1 virus were analyzed. Among those tested, GAT (n = 225), GGT (n = 3), AAT (n = 2), and GAA (n = 1) were individual variants (Table 3). In addition, 10 specimens (4.1%) contained more than a single variant (Table 3). These mixtures would not have been identified using the cyclic dispensation order.
In this study we developed a sensitive assay, utilizing the quantitative nature of pyrosequencing technology, for the detection of individual and mixed variants at position 222 in HA of the pH1N1 2009 influenza virus. Pyrosequencing technology measures a light signal produced after a nucleotide is incorporated into the sequence by the polymerase (21). When a nucleotide is dispensed but not incorporated, a gap is formed in the pyrogram (Fig. 1). We demonstrated that the SQA protocol with a customized nucleotide dispensation order, designed to determine virus variants and their proportions, was more informative than the traditional Sanger sequencing method as well as the pyrosequencing with the cyclic nucleotide dispensation order. Although the SNP pyrosequencing assay is a powerful tool for polymorphism quantification, it cannot be applied to unknown nucleotide polymorphisms. Furthermore, the PyroMark ID software does not permit the SNP analysis of identical nucleotide polymorphisms present at two or more consecutive positions. The advantage of the customized dispensation order was seen when mixtures that would be misinterpreted on the basis of pyrosequencing with the cyclic nucleotide dispensation order were clearly identified in the clinical specimens. No AGT (S) variants were detected in this study utilizing either dispensation order, although it was a possible variant if only Sanger sequencing is used.
The quantitative nature of pyrosequencing allowed the calculation of virus variant proportions with the use of the peak heights within a pyrogram generated using the customized dispensation order. The results from three different approaches, SNP analysis, cloning, and SQA analysis, were in good agreement and supported the use of the formula (Tn − TBkg)/(T2 + T4 + T6 − 3 × TBkg) × 100% = SNPn for the calculation of the virus variant proportions based on peak heights on SQA programs.
The customized nucleotide dispensation order approach has already proven useful in detecting the emergence of variants in other viruses, e.g., in analyzing the transition of wild-type to lamivudine-resistant variants in hepatitis B viruses (15). In this study, we were able to further expand the application of a customized nucleotide dispensation order to the quantitation of each of the virus variants in a mixture.
Pyrosequencing technology has been actively employed for the analysis of the influenza virus genome and has proven to be a robust platform for screening mutations associated with drug resistance in M2 and NA genes (5). The new approach extends the usefulness of pyrosequencing in the detection of multiple virus genomic variants for position 222 in HA. In addition, it was shown to be more informative than Sanger sequencing for quantitative analysis of a polymorphic site. It allows the circumvention of the time-consuming and costly cloning of RT-PCR products from specimens containing virus variants. This approach can also be useful in identifying the presence of multiple variants within influenza viruses and other pathogens. In the future, this assay could be used to rapidly screen for mutations at position 222 of HA or customized to screen for other mutations.
We thank our colleagues from Sequencing Activity, Influenza Division, CDC, for their valuable technical assistance on the project. We also thank Alicia Fry and Marie-Joelle Miron for their useful discussions and contributions.
The findings and conclusions of this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.
- Received 29 November 2010.
- Returned for modification 4 January 2011.
- Accepted 28 January 2011.
- Accepted manuscript posted online 9 February 2011.
- Copyright © 2011, American Society for Microbiology


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There are many ways to get people onto your list. Lead magnets are one such resource. For example, you can build ebooks, checklists and cheat sheets. But you can also do content upgrades, such as PDF versions of an article with added resources in them, four-part video training series, and more. Think about your audience and what you can offer them to better serve them, then treat them with some respect and you'll eventually reap the rewards.
A general rule is to choose a product with Gravity score of at least 20. If you can find one of more than 50, even better. But be careful when you get into the triple digits. This means that the product is having strong competition, so make sure you're prepared. You can also read reviews of products to measure public opinion. Are there a lot of complaints? Are there enough amounts of refunds? Make sure the answer is no.
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If you've got experience and expertise in a specific area of business, there are hundreds if not thousands of business owners who would love to jump on a phone call and pick your brain. This can be an easy way to make extra money online and to hone your skills to use in your consulting or freelance business. Sites like Clarity.fm let you create a profile where entrepreneurs and business owners can pre-pay to book a phone call with you for a set amount of time. Whether you're an expert in raising money for startups, building apps, or running a restaurant, you can make extra money selling a few minutes of your time to people in need.
Fiverr is one of the biggest providers in the gig economy on the web and you can sell a wide variety of services and products through this medium. Do the research and find out what you can offer. However, keep in mind that like any other money-making task, it takes time to succeed here. And stellar reviews will help you generate more and more income over time.
Set up a roadside stand. Depending on where you live, you could profit handsomely by setting up a roadside stand. If you live near a resort area, for example, you could buy cases of bottled water, put them on ice, and sell them to passers by for twice what you paid. Selling fruit and produce you grow yourself is also a smart idea in highly-traveled areas.
It is important to keep your expectations to generate dividends but to make money with Clickbank is not as simple as grabbing a lot of affiliate links, place on your blog and see the package of bills. To make the most of the ClickBank, there are some things you should know, and some strategies to be followed; today I will try to give you some light on the matter. Pay attention.
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A general rule is to choose a product with Gravity score of at least 20. If you can find one of more than 50, even better. But be careful when you get into the triple digits. This means that the product is having strong competition, so make sure you're prepared. You can also read reviews of products to measure public opinion. Are there a lot of complaints? Are there enough amounts of refunds? Make sure the answer is no.
Salvage and resell. Do you love antiques or have a knack for finding valuables at flea markets or yard sales? If you do, it might be time to consider salvaging items for resale – or even scouting out antiques to sell for a profit. While you'll need to spend quite a bit of time searching for prospects and spend some money buying upfront, you could easily turn a profit if you know what you're doing.
Selling digital products can be just as successful as selling physical products. Graphics, software, eBooks, design elements, sound effects, songs, and videos are just a few types of digital products that are in popular demand. So if you can turn your hand to creating digital products, let's have a look at how you can make money online from this skill…
Travel blogging is a hard industry to break into. However, the rewards can be worth it, and let's face it, who doesn't want to be paid for traveling around the world! There are numerous ways to monetize a travel blog, but affiliate marketing should predominantly be your focus. On your blog, promote hotels, tours, equipment hire services, flights, and anything else travel related. Then take a cut of the proceeds as your audience books their holidays based on your recommendations.
Get paid to search the Web. Sites like Swagbucks.com and Zoombucks.com will pay you to use their online interface to search the web. To qualify, you need to be willing to download their search bar and use it for everyday Internet use. The only caveat that comes with this "gig" is that you might be paid in gift cards instead of cash. If you can parlay those gift cards into items you need to buy anyway – like groceries or gas – searching online can be a lucrative way to spend your free time.
Medium is an online community where anyone can post articles and essays and get them in front of their audience of millions of monthly readers. While it can be a great place to build your audience for your own blog or find customers for your consulting business, with Medium's new Partner Program, you can now make money online whenever someone reads your articles. Register for free and you can choose whether your articles are freely available or only readable by those people paying $5/month for a premium Medium membership. You'll make money based on the amount of people who read and engage with your posts each month.
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Set up a roadside stand. Depending on where you live, you could profit handsomely by setting up a roadside stand. If you live near a resort area, for example, you could buy cases of bottled water, put them on ice, and sell them to passers by for twice what you paid. Selling fruit and produce you grow yourself is also a smart idea in highly-traveled areas.
My situation is I'm a Professor and Program Director for a Community College teaching massage therapy, a very in-demand field yet my college does little marketing. It's my job to recruit students. I have a Facebook page and Twitter, and about 100+ followers on each. Can you see this type of system with funnels etc. working to help recruit potential students? If so, how? I feel like building content and creating opt ins should help, as we too are offering a "product". Thanks for your time!
With personal finance software you can schedule automatic payments and transfers between all of your imported accounts. Automatic transfers will help to make sure you have the necessary funds in the right account to ensure all bills are paid on the appropriate date. Late fees are annoying and do nothing but cost you money. It's time that you said goodbye to them once and for all.
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5. Fiverr – Fiverr is a great place to make a few bucks or spend a few bucks if you need some of the services people offer. Basically, everything is $5. You either pay $5 or charge $5. They call them "gigs." You can offer your services however you choose. If you sell art and you're fine selling pieces for $5 each, that's a gig. If you're a graphic designer and you want to offer your services for $10/hour, simply offer a 30 minute gig. If they need two hours of graphic design, they pay you $20, or $10/hour by buying four gigs.
If it's not clear yet, information and expertise are two of the most consistent and lucrative ways to make extra money online. If you don't like talking on the phone, you can sign up to get paid to answer professional questions on JustAnswer. There are thousands of questions being asked every month from people looking for help from lawyers, doctors, mechanics, vets, and more. To apply, you'll need to supply your professional verification, resume, and a form of identification.
Under no circumstances should any information from this blog be used as replacement for professional financial advice. DollarSprout.com is owned by VTX Capital, LLC and neither are licensed by or affiliated with any third-party marks on this website and third parties do not endorse, authorize, or sponsor our content except where clearly disclosed. DollarSprout.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.
Find your niche partners, collaborators, and champions: As you're creating your course, look for notable people who are also creating content in the space. Look att how their businesses operate and incorporate that into your own plan. You can also reach out to any influencers and make them affiliates for your own course. This way, they'll be incentivized to share your content with their own audiences (which can be a major way to generate your first sales—it helps if you're using one of the best CRMs for small business—and start building your own community!)

subset: commoncrawl


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Fast table involves the rejection of food of animal origin. But those who believe that such limitations make a vegetarian menu is boring and monotonous, is not right. Because of the usual vegetables and grains you can cook anything. Even the incredibly delicious meatless burgers, which by their taste characteristics absolutely will not be inferior to meat cutlets. How to do it – tell the site "ToKnow365.top".
How to cook meatless meatballs: a couple of little tricks
- Lean diet burgers anyone will surprise with its diversity. For their preparation instead of meat we can use potatoes, cabbage, lentils, buckwheat, beans, mushrooms, pumpkin, beets and even Hercules. If you wish, you can always make new cake, thus contributing to the diversity of their menu.
- The usual burgers we cook with egg yolks, whose main task – not to allow the meat to crumble. Because during lent eggs we can use, instead of them we will need rolled oats or semolina. These ingredients will also help you to "glue" vegetable patties.
- The main feature of meatless meatballs is that when cooking you can endlessly experiment with the ingredients, adding and eliminating such of them as onions, garlic, carrots, tomatoes and various herbs. All these additives can significantly enhance the taste of your dish.
You and your family will surely like lean burgers, the recipes of which we offer below.
Cabbage meatballs lean
To prepare these cutlets, we need 1 kg of cabbage, half a Cup of semolina, olive oil, bread crumbs, salt and spices.
For a start, finely chop the cabbage and stew it on low heat until soft. Then slowly pour into it semolina and another ten minutes to simmer, constantly stirring. After the cabbage is finally ready, remove from the heat and let cool.
Form of cabbage of the mass of the patties. Next you need to roll cutlets in breadcrumbs and fry in olive oil.
No doubt the burgers will surprise you with its taste. Very well it goes to regular tomato juice.
Carrot cakes vegetable
For these cakes you need to prepare 1 kg of carrots, half a Cup of semolina, half a Cup of water, 1 onion, olive oil, bread crumbs, salt and spices.
Cooking carrot cakes similar to the preparation of vegetable cutlets from cabbage. You just need to put out until half-cooked carrots and onions, adding the pre-in a frying pan or a saucepan with a little water.
Before removing the carrots from the heat, add a bit of semolina and stir carefully.
Form into neat cutlets and continue as in the previous recipe — in breadcrumbs, roll and fry in olive oil.
Very beneficial taste carrot cakes emphasizes specially prepared sauce. Make it very easy – just mix the honey, mustard and crushed walnuts.
Buckwheat burgers lean
For these cakes you need the following ingredients – Cup of buckwheat groats, 300 g mushrooms (e.g. button mushrooms), 1 onion, 1 carrot, a couple of slices of rye bread, salt and spices to taste.
To start, boil the buckwheat and let it cool. Meanwhile, fry the mushrooms with onions and carrots. Connect buckwheat and mushrooms, add soaked in water, bread, salt and pepper to taste.
Shape patties, and then as usual — in the breadcrumbs and into the pan with olive oil.
Perfect for these cakes fit the Bechamel sauce. Make it too easy – dissolve 50 grams of flour in water and stir the mixture in the pan, until the mixture has reached the desired thick consistency. Season with salt, add lemon juice and nutmeg.
As we can see, to prepare tasty meatless meatballs is a snap. And their species much more than we can imagine. Wherefore, do not be afraid to experiment and continue to delight their loved ones with new culinary delights even in the post.
More ideas meatless dishes, see the other article on "Beautiful and Successful".
The website sympaty.net wishing you culinary inspiration!
The author – Tamara Belchenko, site ToKnow365.top
How to cook meatless burgers made from vegetables and grains?


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Menu Development and Recipe Analysis
Realtime Nutrition's team of Registered Dietitian-Nutritionists can develop meal plans and recipes that highlight and feature your products. Consumers appreciate simple solutions to help them incorporate good nutrition into their busy lives.
We provide nutrition analysis not only of the menus and recipes that we create but can also do so for your existing recipes. We use ESHA Research Food Processor Nutrition Analysis Software.


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Here at Gonerby Hill Foot, music is a key part of our school life. Our school builds links through singing and music between school, home and the wider community. Our regular services at St Sebastian's Church are always a celebration of music.
Our children sing on a daily basis as part of our collective worship and sing a wide variety of hymns and songs, both traditional and modern. Our school hymn 'Everywhere Around Me' is regularly sung across the school with clear, proud voices.
Our children have represented the school at a wide range of musical events, covering a wide range of genres from classical to modern pop and jazz! For example, the school choir have proudly represented the school at the Walton Girls High School Choir Festival singing traditional Christmas carols and also preforming a piece of Choral Poetry. Last year they sang as part of the Diocese's first ever Christmas Service at Lincoln Cathedral. The children worked exceptionally hard in the run up to the service and performed beautifully. They were given the opportunity to sing a coral piece by John Rutter in St Hugh's Quire.
The school is also proud to take a large choir to the Young Voices concert every year. Children who take part in this have a fantastic opportunity to sing in one of the largest children's choirs in the country. The children who have attended describe their night at the Sheffield Motorpoint Area as a once in a lifetime opportunity that they will never forget.
In addition to representing our school at these events, our children confidently perform in school on an annual basis from Reception onwards! At Christmas time the Reception classes and the Year 1/2 children each put on an annual performance which always brings a tear to the eye of many proud parents.
Following on from this, the Year 3/4 children always put on a fantastic Easter production. Over recent years they have tackled a musical extravaganza all about Robin Hood, 'Hoodwinked' and in this last year their own version of the classic 'Joseph and His Amazing Technicolor Dreamcoat'.
Finally the Year 5/6 children take to the stage in the summer term producing a fantastic performance to round off our musical year. Over recent years they have had fantastic success with 'Cinderella Rockafella' and 'The Pirates of the Currybean'; shows that were definitely not to be missed.
Extra–curricular music
Brass and Woodwind Tuition
Mr White from Music for Schools teaches Brass and Woodwind instruments. For more information on this service please visit Music for Schools website.
Violin Tuition
Mrs Riley from the Lincolnshire Music Service teaches violin. For more information on this and other musical opportunities in the county, please visit Lincolnshire Music Services website.
Lower Strings Tuition
Cello and Double Bass is taught within school by Mr Green from the Lincolnshire Music Serviice. For more information on this and other musical opportunities in the county, please visit Lincolnshire Music Services website.
There is usually an open evening in the autumn term for children to try a musical instrument and to give information for parents about how lessons work and the options available.


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Atlanta, Ga. (30 September 2016) – After taking a wild spin at one of the fastest sections of the track, TeamTGM rallied back from being almost two laps down to finish sixth in the season-finale IMSA Continental Tire SportsCar Challenge race at Road Atlanta.
Viewing entries tagged
Team TGM
Austin, Texas (16 September 2016) – Even though there are two long straightaways, some 20 corners and a remarkable stadium tunnel that marks the front stretch, the signature segment of the 3.4-mile Circuit of the Americas road course is its long uphill run into Turn 1.
Unfortunately, for TeamTGM Turn 1 wasn't the only uphill climb of the weekend as the squad took on Friday's IMSA Continental Tire SportsCar Challenge race.
Alton, Va. (27 August 2016) – With hot temperatures and sunny skies surrounding the Danville, Virginia area all weekend, the story throughout the IMSA Continental Tire SportsCar Challenge event had been the brutally hot weather conditions. But when the green flag flew for Saturday's Oak Tree Grand Prix, a new story quickly emerged as a heavy downpour abruptly developed over the circuit just 20 minutes into the 2.5-hour event.
Tasked with sharing his commentary from his race weekends in the Continental Tire Sportscar Challenge with Team TGM, Guy files his first 'Debrief' with leading sportscar news website Sportscar365.com.
ATLANTA – Veteran driver Guy Cosmo will join long-time TGM driver and team owner Ted Giovanis for the remainder of the 2016 season. Cosmo will co-drive the team's JKTG Foundation/Camp Boggy Creek #64 Porsche Cayman GT4 Clubsport for the IMSA Continental Tire SportsCar Challenge in the GS class.


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Benew Designs, Inc. is a one-stop exhibition stand solutions company. For exhibit designs, trade show booths, furniture, and interior environments, BD are trusted by a long list of satisfied clients. We also continue to be of service to big and growing companies for the design solutions that they require.
Interior Design
Swedish Designs specialize in delivering world-class custom built exhibition stand and retail environment displays. Apart from producing top-quality tradeshow displays, Swedish Designs is renowned for designing and creating functional space plan.
Built Spaces Contracting, Inc. has carved a good reputation as a builder of finest homes and structures using quality materials and expert workmanship. Specifically, the company designs build, extends, and renovates houses, offices, and other types of low-rise building to provide the most convenient and modern-looking facilities matching the needs of the current environment.
Real Estate and Property Listings
All Properties offer listings of real estate properties all over the Philippines, whether it is residential & vacant lots, condominiums, commercial lots, townhouse, etc. All Properties help you find a perfect home at the right place.
Graphic Design & Print
Retro Creative Advertising started as a graphic division of the Angeles Holdings subsidiaries. With the growing demand in the creative industry, Retro Creative opens its door as a standalone company specializing in graphic design, illustration, and printing.
Hardware Supplies
In a mission to provide systematic and better hardware services, Angeles Holdings partners with Holcim Phils. that comes into a fruition of Holcim HUB Builders Center San Pedro, Laguna—a modern community hardware store that provides premium-quality architectural materials e.g. construction cement, woods, boards, and reinforcing steel bars.
Glass Installation
GlassTechno is a glass and aluminum installation supplier that specializes in fixed glass panels, office partitions, sliding windows, and doors. They also offer glass repairs such as re-alignment of glass door & aluminum Glass, replacement of glass door hinges etc.


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Set of 5 aluminium (Single Ring) chainring bolts. Colour - ANODISED GOLD (as pictured).NARROW - For Single Ring Use (Standard fitment for all types of chainset).For use with a 5mm Allen key (Hex key).Suitable for use with 4 or 5 bolt chainsets.Granny ring bolts are not included. Made from high quality 7075 aluminium.
Alloy Chainring bolts x5 Single Ring GOLD Free UK Postage TJRJCXRQZ


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Metropolis - Solid Mango Wood 6-Drawer Double Chest
In stock
More about delivery and free returns
Rigour and minimalism are on the agenda with the METROPOLIS Mango Wood 6-Drawer Double Chest! With its straight silhouette and solid mango wood frame, this storage chest will prove sturdy and hard-wearing. Practical with its six drawers, it has a large storage capacity and will willingly offer a home to your clothes, bed linen and towels. Set a large mirror on top and this chest can also serve as a dressing table!
Frame, front of drawers, side and top panels: solid mango, light stained finish
Bottom, back panels: acacia and plywood, light stained finish
Drawer inners: mango and plywood
Handles and feet (unassembled): metal, patinated and grey finish, satin-finish polyurethane varnish
6 drawers on metal runners
This product is recyclable. When it comes to the end of its useful life, remember to take it to a recycling centre.


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The Tire Pressure Monitoring System (TPMS) tracks the amount of pressure in tires and alerts a driver when the pressure is at an insufficient level. There are two types of tire pressure monitoring systems—direct and indirect. A direct TPMS receives information from pressure sensors inside each tire on the vehicle, while an indirect TPMS uses speed sensor data from the wheels to determine tire pressure levels. When a tire has an insufficient level of pressure, it becomes a safety hazard for various reasons. If pressure is too high, tires tend to bounce and lose traction. If pressure is too low, tires heat up quickly and wear out easily. Heat can negatively affect the tread of a tire and ultimately lead to blowouts and accidents. We recommend that drivers pay attention to the Tire Pressure Monitoring System and heed any warnings it produces.
When your TPMS alerts you to a tire pressure problem, please contact us for assistance. Our service staff is equipped with the right tools for the job. After we use our professional diagnostic tools to obtain information from your TPMS, we can help you inflate or deflate your tires until they are within the correct pressure range. We can also check that your pressure or speed sensors are working correctly. In the case that your Tire Pressure Monitoring System is simply not working at all, our service staff will determine the correct repair procedure. If you have any questions about the functionality of your TPMS, please give us a call or contact us online. We are happy to discuss your TPMS with you and look forward to sending you back out on the road with a fully operational system.
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John Armstrong (O.P.), Debater (St Pauls School). People are intolerant of insults, even if unintentional, and react by bringing them to the attention of authorities or to the public at large. David Hume: Bicentennial Papers (Edinburgh, 1977: Edinburgh University Press 93116; repr.
Bibliography - Isaiah Berlin
Oakeshott rationalism in politics and other essays
Improvements to the texts or notes will be gratefully received. The Essential Chaim Weizmann: The Man, the Statesman, the Scientist (London, 1982: Wei Nicolson originally to have been reprinted in PI, but IB changed his mind; repr. 21 Pythagoras (570495 BCE) edit Main article: Pythagoras Pythagoras was one of the first Western philosophers to stress rationalist insight. As Isaiah Berlin Against the Current, New York Review of Books, 166aEinstein and Israel, New York Review of Books, 8 November 1979, 1318; repr. As Appendice sulla teoria del Vico circa la conoscenza storica in Vittore Branca (ed. 12, 63850 (a quite outstanding article, The Times Literary Supplement, 486 revised in 1966. From the rationalists perspective, for instance, politics consists of designing institutions according to abstract principles, without any regard for culture and tradition. Hamann (17301788) (French trans. On History can be interpreted as an essentially neo-Kantian enterprise of working out the conditions of the possibility of historical knowledge, work that Dilthey had begun. Michael, oakeshott, in full, michael Joseph, oakeshott, (born December 11, 1901, Chelsfield, Kent, Englanddied December 18, 1990, Acton, Dorset British political theorist, philosopher, and educator whose work belongs to the philosophical tradition of objective idealism.
16 (May 1990 67 192cOn Yitzhak Sadeh (in Hebrew translation Davar, 5 September 1986, 17; incorporated into 214; trans. Cummings, M E (September 8, 1929). Survey of Jewish Affairs 1983 (Rutherford/ Madison/Teaneck, 1985: Fairleigh Dickinson University Press; London/Toronto, 1985: Associated University Presses 23843; excerpted in Jewish Chronicle, ; repr. Henry Hardy, foreword by Alan Ryan and afterword and (revised) guide to further reading by Terrell Carver (Princeton and Oxford, 2013: Princeton University Press a thoroughly revised text with references added for"tions 25Verification, Proceedings of the Aristotelian Society 39 (19389 22548; repr.


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The sewing ladies, called the Parish Piecemakers, in St Cedma's in Larne have been very busy stitching over the last number of months and have created a wonderful selection of quilts to celebrate the joy of the Easter season. The reality of the meaning of Easter revealed after the long penitential season of Lent is truly a joyful and celebratory moment! These quilts will be on display in church on Easter Sunday for the services and then the church will be open in the afternoon between 2:00 - 4:00pm for anyone who wishes to come and look more closely and enjoy the atmosphere . (On this occasion there will be no refreshments served.) The quilts will also be on display on Saturday, 27th April and Sunday, 28th April, from 2:00 - 4:00pm on both days with refreshments being available on both days for a nominal charge.
Back to All Events
Earlier Event: 21 AprilEaster Sunday - The Feast of the Resurrection
Later Event: 24 AprilWedding Day


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A look at the early influences that made David Bowie a music icon
A look at the early influences that made David Bowie an icon
This is the third Bowie film from Francis Whately, whose previous offerings looked at the singer's final recordings and reinventions.
Here, he traces Bowie's early life, and we hear from the likes of childhood pal George Underwood – whose punch led to one of David's eyes changing colour – and, in her only filmed interview, his first love, Hermione Farthingale.
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There's even some rare Ziggy Stardust footage thought to have been 'lost for ever'.
It's a picture of a hugely ambitious young man, keen to experiment (both with music and make-up!), though perhaps The Laughing Gnome was one experiment best forgotten.
Catch a repeat of Bowie's 2000 Glastonbury set on BBC4, 10.55pm.
TV Times rating: *****


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The case of the rotting fisherman
Richard Bauckham offers vivid demonstration of how reliable eyewitness testimony can be. I was particularly drawn to this pair of accounts relating to the same incident, because it took place in my home county of Norfolk, England.
In June, 1901 a local newspaper carried the following report:
Seventy-two years later, in 1973, a man recalled this very event in an interview exploring village practices in the past:-
Following Bauckham's lead, we may make a number of observations:-
The newspaper account (published within one week of the event), and the personal recollection (recounted after more than 70 years) are entirely compatible with one another.…


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Come into Your Gifting
June 29, 2012
2 Timothy 1:6-7
For this reason I remind you to fan into flame the gift of God, which is in you through the laying on of my hands. For God did not give us a spirit of timidity, but a spirit of power, of love and of self-discipline.
We know that the word teaches that to each one of who possess the Holy Spirit within us, we have gifts imparted to us through God. Some of us know full well what they are and some of us may still be discovering them. The important thing in this hour is to know that God has placed certain things in you for your ministry to the body and the world at large. Often times, even if we know what some of our giftings are, we may not be moving in or exercising them.
Sadly, many of our churches do little to really stir up the gifts within us and help us to exercise and develop into the spiritual maturity of them. What is in my spirit is that God is wanting to greater activate His body in this hour and bring her into a greater maturity. So much of the church has been kept dumbed down with listening to others and perhaps doing religious activities, but not really moving out in the gifting and abilities that God has placed within each true believer. God is wanting to activate you. He is wanting to stir up that gift(s) inside of you and bring them to fruition and demonstration.
It is important to remember that the gifts are nothing in themselves unless they operating out of love in the power of the Holy Spirit. My sense is that God is beginning to baptize us into a greater revelation, expression and demonstration of the Agape' love of God than we have ever experienced before. Get ready, because in this love, self is lost and it becomes all about Jesus and the expression of who He is. For so long, most of our religious experience has been all about us. What meets our needs, how we like to worship and what we want to hear. When we don't get what we want or someone offends us by what they say or how they act, we simply go somewhere else and the process starts all over. It is important today to ask God where He wants you and to be faithful and committed to that place until He releases you or moves you elsewhere. Remember it may not be everything you want. It may not tickle your ears. You may even find offense and discomfort there, but instead of leaving and running away you need to stay and grow through it. You grow through it by exercising the love of Christ with all of its attributes. You will never come into that if you are always pursuing the path of least resistance.
What is important for each of us as believers is to begin to discern and recognize the giftings in one another. As we recognize these, we need to acknowledge and affirm them in our brothers and sisters, encouraging them and stirring them up to operate and exercise the giftings that they carry. The spirit of timidity and fear is reluctant to express itself for fear of failure or rejection. We need to create a culture of love that provides a safe place for others to develop and come into their gifts. Obviously this is a maturing process and mistakes can be made along the way. The mistakes are not to our detriment, but for our growth. Don't be afraid of rejection and failure. Step out in what you know and have by faith. Holy Spirit will help you fine tune it along the way. The main thing is always operate your gift out of love, the ultimate edification of others and glory of God. Our gifts can be a two-edged sword with which we can wound, maim and kill as well as give life and blessing. How it is used depends upon your heart and the love of God that fills it.
As we step into this arena of using the weaponry of our gifts, we cast off of us the spirit of fear and timidity as we embrace the spirit of power, of love and the sound mind of discipline that should go with those gifts and abilities. They should not be neglected, nor should they be used without the proper leading of the Holy Spirit in the attitude and heart of love.
God wants to stir up your gift today. Don't fear it, embrace it, but along with it embrace His power, His love and the sound mind of discipline in how to properly operate in it to give life and do the work of God through it.


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Passivity is a CurseLeave a comment
December 12, 2015 by wimvincken
On broad daylight on a public beach, full of people, a girl of 13 was drugged and gang raped by …
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November 17, 2015 by wimvincken
The refugee problems from the Middle East and Africa is not only a potential security problem (hidden terrorists, Islamic extremists, etc.), but they get mismanaged in their new host countries as well. The normal political correct approach of the authorities go too far to let those people and the local population suffer because of incompetence of the politicians and their ability to make things happen. Who is crazy enough to accept more then 800,000 additional refugees when they are not able to handle the current number of refugees? It has all the signs of disasters going to happen and I'm not talking about security disasters only, but social and human disasters.


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An Uncertain Financial Future Worries Canadians Amid Bank of Canada Rate Hike
Canadians need to prepare for a changing financial landscape that includes higher interest rates.
On January 17, the Bank of Canada raised the key interest rate from 1 to 1.25 per cent, which led most major banks to raise their lending rates 25 points as well. This means borrowers will likely pay more when getting a mortgage or loan, but savers will also see a bonus in the interest they earn. It's the central bank's highest interest rate since 2009 and, as this is the third time since July 2017 there has been an increase, there are worries this is just the beginning.
These worries, that there's more rate hikes to come, is already having a big effect on the financial confidence of Canadians.
Increased rates cause Canadians to worry about their financial future
CIBC published its annual financial confidence survey just prior to the Bank of Canada announcement and, at the time, 77 per cent of Canadians said they were confident about reaching their financial goals this year. About 70 per cent were also positive about their financial situation, even though an equal percentage said they had concerns about higher food and utility costs. Some of the reasons why people were confident about their finances included: the rising stock market, the raise in minimum wage, and moderate economic growth.
However, that positivity wavered once asked about a potential interest rate hike, with 59 per cent saying they would be significantly less confident if (and now when) rates increased. Another recent survey by CIBC showed two-thirds of people wanted a better handle of their finances and half wish they'd paid down more of their debt when interest rates were so low.
This worry is also shown in a similar recent Ipsos poll by MNP Ltd., which revealed that more Canadians are worried about their debts right now. Almost half of those surveyed (48 per cent) said they are $200 or less from being able to make ends meet and 33 per cent are unable to cover monthly bills and debt repayments. As a result, four in 10 (42 per cent) expressed concern that if interest rates increased much more they would be in financial trouble.
Minimize the worry by planning now
If you're struggling with your finances, especially after the interest rate change, CIBC offers the following tips to help you meet your financial priorities:
- Create a financial plan that itemizes your income and expenses so you can see where your money goes.
- Focus on paying off any high-interest debt you have, or consider consolidating your debts into one loan.
- Evaluate your spending and identify areas where you may be able pare back discretionary expenses.
- Start saving now. Even if it is a small amount, set aside some money from each paycheque into a separate account.
- Get an expert opinion from someone who can help you put realistic steps in place so you can make real progress toward improving your financial situation.
If you're looking to save money wherever possible, amid this rate hike, Rates.ca can help. From paying less for your auto insurance to finding the best mortgage that's the right fit for your life and budget, shop around to ensure you're getting the best deal available.


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As reported earlier, Electronic Arts will be showcasing gameplay from their upcoming title Star Wars Jedi: Fallen Order during their presentation at EA Play 2019. The 2-day event is scheduled for this weekend June 8-9 in Los Angeles, California. We knew from our initial post that the game would be part of the EA Play action, but we did not know exactly when Fallen Order would hit the EA livestream from the event.
We do now as you'll be able to catch Star Wars Jedi: Fallen Order on the livestream beginning at 9:30am PDT on Saturday, June 8. Electronic Arts has released their schedule of livestream events which is copied below.
For those that can't make it out to Hollywood, we have a whole lot of content for fans to get into. We're taking you deeper into our games this year with three hours of live stream content on Saturday, June 8, bringing you gameplay and insights from six of our biggest franchises. It all starts at 9:15 a.m. with the Countdown to EA PLAY followed by the franchise live streams.
Here's the schedule for the live streams (all times PDT):
9:15 am: Countdown to EA PLAY
9:30am: Star Wars™ Jedi: Fallen Order™ (Hosted by online personalities Greg Miller and Andrea Rene)
10:00am: Apex Legends (Hosted by commentator Alex "Goldenboy" Mendez)
10:30am: Battlefield V (Hosted by online personality Julia Hardy and EA's Adam Freeman)
11:00am: EA SPORTS FIFA (Hosted by Alex "Goldenboy" Mendez)
11:30am: EA SPORTS Madden NFL (Hosted by NFL Network's Adam Rank)
12:00pm: The Sims 4 (Hosted by Andrea Rene)
To watch, be sure to tune in to EA.com or EA's YouTube or Twitch channels. If you are only interested in a couple of the games, you can also tune in at the specific times listed. For all EA PLAY content throughout the weekend, including exclusive gameplay reveals, trailers and breaking news, be sure to stay locked to EA.com.


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Considerable Quotes
ZEUS, n. The chief of Grecian gods, adored by the Romans as Jupiter and by the modern Americans as God, Gold, Mob and Dog. Some explorers who have touched upon the shores of America, and one who professes to have penetrated a considerable distance to the interior, have thought that these four names stand for as many distinct deities, but in his monumental work on Surviving Faiths, Frumpp insists that the natives are monotheists, each having no other god than himself, whom he worships under many sacred names. - Ambrose Bierce
With the ongoing political tensions in Iran and with Nigerian militants threatening more attacks on oil companies, short covering should push prices higher next week. Speculators still hold a considerable amount of short positions. With the current news flow, those investors should feel increasingly uncomfortable with their positions. - Tobias Merath
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Manchester United boss Louis van Gaal has warned Marcos Rojo he still has room for improvement as a defender even at 25.
The Dutchman was speaking on Tuesday at a pre-match press conference ahead of his side's Champions League match against CSKA Moscow after he was bombarded with questions by a Russian journalist.
Rojo had played for Spartak Moscow before moving to Sporting Lisbon but the journalist claimed the Argentine wasn't good enough at the time but Van Gaal, who defended the 2014 World Cup finalist, described him as a fast and physically strong defender at the Arena Kimkhi.
Rojo joined Spartak in December 2010 but only made 19 first-team appearances before completing a transfer to Sporting in July 2012.
"I didn't know that he played for Spartak Moscow, but I have seen him in the World Cup in Brazil when he played for Argentina," Van Gaal explained.
"He did it very well and he also played for Sporting Lisbon. There I analysed him. What I like is that he is physically a very strong guy, he has a good header of the ball and his pass is very fast.
"That is what we always need, we need a high speed in our positional game. When he improves and he can reach a very high level because he is not fully developed yet. I think there is great potential in him to become a very good player for Manchester United."


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Artificial Sweeteners Are Bad For You...True or False?
Today's episode is all about artificial sweeteners.
Do you know what they are?
Do you know what happens in your body when you ingest them instead of sugar?
Do you know what the trickle down effects are or can be?
Tune in to this episode for all of this and more!
Cited Studies & Resources:
Molecules 2018, 23(10), 2454; https://doi.org/10.3390/molecules23102454
Don't forget to check me out on the platforms below.
Listen & Download below or find it on Anchor, Podbean, Stitcher, iTunes or Spotify @Light Body Radio
There is lots more on the way! Don't miss it!
Follow me @drlaramay on Facebook, Instagram, Twitter & Youtube.
Hi! I'm Lara (pronounced like Sarah, but with a L).
Are you ready to lose weight, reduce stress, and reinvigorate your life?!
Together let's uncover your motivators for change, and turn your vision for your vibrant future into your new reality. Health coaching is a safe supportive process of aligning your dreams with your intentions, and taking small right actions toward reaching attainable goals every week. I am here to gently hold you accountable with action plans, assignments, support and feedback.


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Schroder High Yield Opportunities Z Inc
Fund Info
- Yield History6.60%
- 3 Year sharpe1.13
- 3 Year alpha-2.3
- Distribution Typeincome
- BenchmarkN/A
- Legal StructureUnit Trust
- Fund Size (month end)Unit Trust
- Sector£ High Yield
- Manager GroupSchroders
- DomicileUnited Kingdom
Investment Strategy
The Fund aims to provide income by investing in fixed and floating rate securities worldwide. The Fund also aims to provide capital growth. The Fund invests at least 80% of its assets in fixed and floating rate securities (denominated in or hedged back into Sterling) issued by governments, government agencies, supra-nationals and companies worldwide. As the Fund is index-unconstrained it is managed without reference to an index. The Fund invests at least 50% of its assets in Pan-European fixed and floating rate securities.


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Golden Boy Promotions Begins 2012 with ShoBox Doubleheader at Fantasy Springs Resort Casino
by Press Releases on Jan.06, 2012, under Boxing News, Press Releases
This Friday's Card Features Luis Ramos vs. Raymundo Beltran and Michael Perez vs. Omar Figueroa, Plus Four Exciting Undercard Fights
LOS ANGELES, January 5, 2012 – The 2012 boxing season gets off to a rousing start this Friday night at Fantasy Springs Resort Casino in Indio, California, when Golden Boy Promotions and SHOWTIME present a hard-hitting doubleheader featuring the WBA Fedelatin lightweight title fight between undefeated Luis Ramos Jr. and veteran Raymundo Beltran and a battle for the vacant WBO Intercontinental Youth lightweight crown between unbeatens Michael Perez and Omar Figueroa Jr.
Before the SHOWTIME cameras deliver the first ShoBox: The New Generation of 2012 to boxing fans across the country, fans in Indio will be treated to a full slate of undercard action headlined by the return of unbeaten Las Vegas knockout artist Michael Finney.
Ramos vs. Beltran and Perez vs. Figueroa are presented by Golden Boy Promotions, sponsored by Corona, DeWalt Tools and AT&T and will be televised live on ShoBox: The New Generation at 11:00 p.m. ET/PT (delayed on the West Coast) on SHOWTIME free during the premium network's Free Preview Weekend, available in more than 60 million homes.
Tickets, priced at $25, $35 and $45, are on sale now and available for purchase at the Fantasy Springs Box Office, by calling (800) 827-2946 or online at www.fantasyspringsresort.com. Doors at Fantasy Springs will open at 6:00pm PT on fight night and the first bell rings at 6:30pm PT.
Alabama native Michael Finney (7-0, 7 KO's) has settled into his new home in the fight capital of the world, Las Vegas, and with knockout wins in each one of his seven professional fights, the 20-year-old phenom is proving that he as the power to end any fight in an instant. He's ready to kick off his 2012 campaign following his last fight in March of 2011 when he had an impressive knockout over Josh Beeman. Finney is looking to keep his knockout ratio intact when he meets Hopkins, Minnesota's Hector Orozco (4-9) in a six round welterweight bout.
In other undercard action, Troutwood, Ohio's former amateur star Christopher Pearson (1-0, 1 KO) faces Long Beach middleweight Cleven Ishe (3-4, 1 KO) in a four round middleweight battle, Santa Ana's Naphi Mohammad (0-1) will attempt to secure his first professional victory in a four round junior lightweight fight against Las Vegas' Rocco Espinoza (1-2) and Santa Ana's Ricardo Malfavon (1-7-1, 1 KO) takes on Stephen Rubalcava (0-7) of Exetan in a four round middleweight contest.
For more information, visit www.goldenboypromotions.com, www.fantasyspringsresort.com, follow us on Twitter at www.twitter.com/GoldenBoyBoxing, www.twitter.com/fantasysprings or visit us on Facebook at www.facebook.com/GoldenBoyBoxing or www.facebook.com/fantasysprings.


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Wimbledon in the Great War
November 2018
An exhibition produced by Merton Heritage Service for The Wimbledon Society as part of the borough's First World War Centenary programme.
This exhibition tells the fascinating story of Wimbledon during the First World War. It covers a variety of topics including the VCs, recruitment, local regiments and military camps, the Belgian refugees, the wartime role of local women and the invaluable contribution made by Merton hospitals, industries and charities. There are anecdotes and recollections by local people too.
The research behind the exhibition and images on display are from the Merton Heritage Services' Carved in Stone project


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9 Chic Holiday Dresses That Standout For UNDER $100/
The Holidays are a time to standout, sparkle & shine. But with so many parties to attend and gifts to buy finding the right look at the right price can be an added challenge. Here are some looks that will keeping you looking & feeling festive without breaking your budget.Read More


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11 Jun 2019
22 Best Stainless Steel Beer Bottles
Beer Bottle Opener- Magnet-Automatic,Stainless Steel-Push Down Bottle Openers,Bottle Cap Remover(3PCS) - The best corkscrew designstainless steel beer bottle opener, automatic and effortlessthis gadget device pops beer and soda caps right off, quick and easy. The business or great christmas gifts for those in your life that drink out of bottlesfun bottle cap removers that entertain your guests or friends on holidays, housewarming and so on. Great for opening beer bottlesif you are a bottle cap collector or crafter ,then you will love this decapitator,which doesn't bend the bottle capno damage to the bottle cap. Light weight,durable material and stainless steelsize 332121 inchesit's not matter what the size is herethis bottle opener is built to heavy duty last and can open bottles many times within a few minutes. Easily and safelyjust put the beer opener onto the bottle and press the top of the opener you just push it down on a bottle, it clicks, and the cap is removed takes less time.
Elloapic Magnet-Automatic Bottle Opener Stainless Steel Beer Bottle Opener for Bottle with Cap (3) - 2well design and made made by high quanlity stainless steel, can keep for long time use fashion and humanized design the surface polished in high-grade, in a very good hand-feelingbetter than slice-design bottle opener very popular among in home, bar, etc. 1an easy to use bottle opener for beer and any other bottle with a cap open caps very fast and safethe opener catcher with magnet, will not let the caps down. 4great for cap collectorsthe opener catcher with with magnet, will not let the caps downwill not bend caps so cap collectors can easily save the caps. 3portable & effortlessly size is 53x53x85cm, small size fit any handsyou do not need much power, but slightly push down the opener, the cap will be out.
Brand New 2L/64oz Home Fashion Stainless Steel Beer Bottle Barrels Beer Keg Beer Growler Homebrew Wine Pot Barware For Party - Unit type piece package weight 1000kg (220lb). Package size 11cm x 11cm x 5cm (433in x 433in x 197in) body material metal. Type mini . Brand new imported. Product will be delivered in 15 to 25 days.
AStorePlus Ring Style Bottle Opener, Stainless Steel Beer Bottle Cap Opener Beer Favorite - Diameter approx 22mm. Great use for open your beer and soda bottles or most bottle cap. Great for parties & bar & bbqs & traveling & as a gift. Fabulous bottle opener comes in the form of finger ring. Material stainless steel.
Stainless Steel Beer Bottle Opener Wine Stopper Bottle Sealers Sparkling Champagne Bottle Saver Cap Bottle Opener Bar Tool - 5cmstopper diameterapprox. 2. Typeopenersfeatureeco-friendlyopeners typebottle openersmetal typestainless steel+siliconeheightapprox. 5. 5cm.
Premium Beer Growler | Single-Wall 2 Liter / 64 oz Flip/Swing-Top Stainless Steel Beer Bottle w/ Etched Logo | by: BEER Is Good For You | Includes: Gift Box, Spare Seal, Growler Tips & Sticker - ✔ this premium beer growler has a high quality 3-part (steel/plastic/silicone) flip-top lid which retains carbonation like a champ. ✔ Included with your purchase is a spare rubber seal (for lid), brand sticker, care/filling instructions all packaged in a branded gift box. ✔ The perfect gift for any craft beer lover leave your brewery in style and show up to your next soiree with the coolest growler in town. ✔ Also available insulating sleeve/carriers & scratch-free brushes in our gift pack from beer is good for you. ✔ Features 1 liter or 338 oz actual fill capacity & made from food grade 304, 18/8 surface treated stainless steel contoured shape with etched logo that will not fade, scratch or peel.
SSGP Stainless Steel Beer Bottle Opener and Can Opener, Wine Opener with Piercing Tip - Slim, compact, and durable. Perfect for any man cave, bar, kitchen, patio, cabin. Easy opening with one hand. Each measures approx 7″ long. So good.
Mhhpek Strong Heavy Duty Chrome Can Opener Stainless Steel Kitchen Craft Stainless Steel Beer Bottle Opener New - Ergonomically designed for comfort and ease when using. Easy to clean since the blades never touch food, you only need to quickly rinse the can opener after each use. Compact desgin and handful usehandy for daily use, family picnics, hiking, camping and fishing. High quality stainless blades will never rust. Revolutionizing new age cutting design cuts tin cans effortlessly leaving smooth edges.
Stainless Steel Beer Bottle Opener - These hand-held bottle openers are made of stainless steel, making them durable enough to keep the tops popping off for years to come. Comes in 7-inch length. This tool is easy to handle and precisely the right tool for the job.
Bottle Opener ,Yerwal Stainless Steel Beer Bottle Opener for Your Wallet(Green Slipper) - Package included1pcs bottle opener. Used to open the beer bottles. Size(hwl)94301cm/3517004inch. Shapegreen slipper. Made of sturdy stainless steel.
AMZTM Stainless Steel Beer Bottle Cooler With Opener, Sweat-Proof Beverage Can Insulator, Portable Bottle Keeper For Beer lovers (Silver) - Portable design the beer cooler could be detached easily and fit most long-neck beer glass bottles,it keep your beer stable inside and the portable design make it easy to carry on,you can take with it just like a water bottle when you go camping,hiking,or any other outdoor activitieswhen you feel tired,take it out and open your beer with the bottle opener on the lid easily,nothing better than enjoying the cold beer at that moment in life. Double walled insulation besides the stainless steel container,there is a neoprene lining protective layer inside the bottlewith the double insulation layer design,the bottle could keep your beer stay cold for a long time and you can have a cozy drink without the annoying "sweat" on the bottle. Great gift choice if you are looking for a beautiful and useful gift for these people who love drinking beer and have a picky taste of cold beer,then this one is your best choiceit's a "must have" one for beer enthusiasts,there is no doubt that they will fall in love with this amazing insulator. Satisfaction guarantee we are committed to providing the best products and services for our customers on amazon you can get a refund at any time if you were not satisfied with our product or service and we will be very glad to accept the suggestion from you. Best temperature you always get the most perfect beer at the temperature of 446 ℉ to 50℉ just like you get the best coke at 392℉for beer enthusiasts,the temperature is always the most important factor to drink beer-only cold beer bring the most wonderful taste and satisfy the desirethis insulator is specially designed for these enthusiasts with its powerful insulation function to keep beer at best temperature.
Premium Wing Waiters Corkscrew Red Wine Opener Professional Multifunctional Stainless Steel Beer Bottle Cap Opener Red - Multiple use & good giftideal for any restaurant, bar, birthday party,or home for a romantic evening or just a casual drink buy a solid heavy duty and built to last wine corkscrew as a great hostess gift for your families. Ergonomic wing handles double wing rubber handle makes this bottle opener comfortable to grasp and effortless to turn, even when you need to use a little more effort than usual to open a stubborn cork, free from injury, allowing you to easily remove any size cork quickly and efficiently. Easy to usethis professional wine bottle opener is foolproof and efficient, simply place the wine corkscrew opener over the bottle, twist the handle to insert the auger into the cork, and watch the opener's wings rise then press the wings down to extract the cork far superior corkscrew, efficiently open many wine bottles for you. Fashion styleexquisite and simple design, smooth lines, beautiful, practical, guarantee never outdated. Wing corkscrew materialconstructed from zinc alloy body and the finest quality copper plated stainless steel corkscrew for strength and flexibility with touch panels and easy grip.
Kloud City Pack of 5 Bottle Cap Opener Stainless Steel Credit Card Size Casino Poker Shaped Beer Bottle Corkscrew - Use the cleverly crafted hole in the center shaped like a spade to easily open your beer and soda bottles. Made of metal poker shaped designs, funny and stylish look design. Perfect tool for your favorite beer lover it's also a great groomsmen gift. Measure approximately 325 x 2 inches, slim style fits in your pocket, easy to storage and take. Durable for different usages in daily life, widely used in kitchens, bars, beach and outside patio dining and hiking.
FaceWood Opener/Cedar wood and stainless steel beer bottle opener 2pcs/personalized text customization/Kitchen supplies, bars, parties, camping. (FaceWood) - Extremely personalized text customization(3pcs), as long as you are in the order to leave a message to us your letter,we will design and make for you free of chargeall your friends who love beer will be very envious of your idea. Because it is natural wood,the grain of wood will not be the same,please do not mind. If you don't need any text customization,please select the default 2 pcs set purchases. Environmentally friendly natural no paint cedar wood,combined with food grade stainless steel,strong and durable. To customize the product,please attach the text to the message and select the number of the font when you buy it(extext+e03).
Bottle Opener – Water Bottle Opener – Multi-Shape Creative Stainless Steel Beer Bottle Opener Portable Mini Key Chain Ring – Baby Bottle Opener( 14) - Material stainless steel. Color silver, cool grey, bronze etc. Bottle opener, water bottle opener, baby bottle opener, christmas gift, provide you multi-shape creative beer bottle opener, meet you various needs it is not only a beer bottle opener, but also a nice key chain ring as a mini decoration around you. Recommand the creative style, like the "like" style, shark, wrech, guitar etc. Perfect to use for open the beer in the party, show your humor and lifestyle.
20 pcs lot Stainless Steel Beer bottle opener Wine Finger Ring Opener for Beer Bar Tool - Type beer. Color silver. Material stainless steel. Quantity 20 piece. Size inner diameter 22mm /thickness 18mm.
Chakemo 3-Piece Discreet Stainless Steel 12oz Beer Bottle Cooler With Keychain Carabiner – Highest Quality Beer Cooler – Metal Beer Keeper - Material, tough 304 stainless steel carabiner and body with 18/8 surface finish, bpa-free plastic threaded loop cap with keychain attached carabiner, inner locking freshness seal, and diving grade scuba suit neoprene insulation. Protects your glass bottles, no more broken glass or spilt drinks with the tough stainless steel constructed body, snug neoprene insulation, and beverage sealable top lid. Cold on the go, the insulated design keeps bottles cool for a long time while the looped cap is ideal for on-the-go carrying to the beach, on the boat, camping or tailgating to your favorite sporting events. Discreet, stainless steel water bottle design hides glass beer bottle to look like an average water bottle great way to conceal your drink for many occasions from the beach to sporting events and bbq's.
Corona Extra Embossed logo Stainless Steel Beer Bottle Opener New - Measures approx 7 inches long. Made of stainless steel. Brand new not sold in stores. Corona extra bartender bottle opener. Corona extra logo embossed on to the metal.
Stella Artois Embossed logo Stainless Steel Beer Bottle Opener New - Brand new. Stella artois bartender's bottle opener. Stella artois logo embossed on to the metal. Made from stainless steel. Measures approx 7 inches long.
Wish you have a nice day black beer Bottle Cap Catcher – Stainless Steel (Cap Catcher, Black) - Features made of 1mm thick stainless steel. Specs dimensions 3 1/2″h x 5 1/2″w x 2 1/2″d. In stock and ready to ship. Includes mounting screws. Predrilled mounting holes. Made of stainless steel. Compliment your wall mounted bottle opener install one of these handy cap catchers underneath to keep the caps from falling to the floor.
Set of 2 Insulated Beer Bottle Holders – Stainless Steel Bottle Holder Beer Chillers with Bottle Openers - Set of 2 stainless steel bottle holder insulators with bpa-free neoprene lining. Fits most 12oz beer bottle brands. A discreet way to enjoy your beer on the go. Beer bottle holder keeps your beverage cold longer bottle stays chilled and protected. Modern, functional and fun 2-pack set makes the perfect gift.
2x Stainless Steel Beer Bottle Holder Insulator With Opener and Carrying Case - Bonus features, comes with a bottle opener attached to the lid to make it easy to open your favorite drink anywhere it also comes with carry case that can easily attach to bags. Protects, built with military grade stainless steel metal to be almost indestructible for keeping your bottle safe during any activity such as rock climbing, boarding, sports, camping, tailgating, etc. Safer, 100% bpa free dish washer safe it keeps your furniture safe from water rings while watching the football game on tv. Cooler, keeps drinks cooler longer because it is lined with an exclusive insulation originally designed by nasa engineers to keep things colder at extreme temperatures while being ultra light weight comes with a carrying case that adds additional insulation. Guaranteed to last, built to last more then a lifetime each bottle comes with a lifetime guarantee we will replace for free get yours today.
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