text:
You are not new to perfumes and you find "designer perfumes" in department stores too simple or crowd-pleasing. The unique, complex and creative scents in this sample pack will give you a nice challenge, and you might find your "signature scent" here!
This Zoologist perfume sample set includes:
Total 9 samples. Each sample 2 mL / 0.06 fl. oz.
Made in Canada. Product will be shipped from Canada.
This Zoologist perfume sample set includes: Beaver Camel Chameleon Civet Dodo Dragonfly Elephant Hummingbird Hyrax Macaque Moth Nightingale Panda Rhinoceros Squid Tyrannosaurus Rex Total 16 samples. Each sample 2 mL /...
This Zoologist perfume sample set includes: Camel Chameleon Civet (Art and Olfaction Awards 2017 Finalist) Elephant (Art and Olfaction Awards 2018 Finalist) Hummingbird Panda Squid Tyrannosaurus Rex Each sample 2 mL...
New to Zoologist? This sample pack contains our unique smelling scents that are easy and a joy to wear. You will most likely get a compliment wearing them! This Zoologist...


text:
What is your favourite or preferred project management system?
These days there are so many management platforms such as Asana, Active Collab, Trello, Podio Teamwork PM. Which one do you use and why?
I used to use Trello, however, I didn't like the UI. I thought it was a little ugly to use, and it was confusing. Plus, they didn't have an app for my phone.
Now my main project management system is Asana an Google Drive. Google Drive is awesome because it lets me collaborate in real time, and plus I can get everything done on Google - email, Google plus, and analytics.
Asana is just beautiful & simple. What more can I say?
We use Asana a lot here at Savvy because it's incredibly powerful in tracking multiple initiatives, but it's also a great team communication tool, even with satellite teams. It can get quite a bit overwhelming if you're not used to working in a project environment, but otherwise it ticks most of the boxes for me.
I use *shudder* Basecamp CLASSIC.
I really want to use something else and constantly evaluate various PM systems that come my way, but the one feature that I simply can't do without is the ability to see a calendar with all milestones for all people across all projects.
Everything else is secondary and can be solved with processes or API customisations (albeit cumbersome to do so in places).
I've seen a few "gantt" plugins that purport to sit on top of Trello etc. but haven't had the chance to evaluate any yet ... if anyone else has some war stories or recommendations I'd love to hear them.
After looking at quite a few including a couple already mentioned and several highly sophisticated (expensive!) tools, I went with Project Bubble.
It fits my main criteria in that it's intuitive to use, shareable and has easily defined tasks. Plus, it's okay to look at.
Based on my experience, Asana is a solid, user- friendly project management tool, I will recommend.

subset: commoncrawl


text:
Welcome To The "Gucci Garden"
Images: Gucci
Gucci has activated its "cool" button again with the release of their new "Gucci Garden" capsule. The new capsule includes some dope bomber jackets, embroidered denim and more. The overall theme of this capsule is the vibrant floral and snake design for a mixture of sweet and edgy.
The ready-to-wear collection not online includes the bomber jacket but also pleated skirts, sneakers, and the beautiful Gucci "Dionysus" handbag. This collection is currently being sold exclusively on Gucci's website.
Metallic uppers make this Thea shine.
NikeLab brings some gorgeousness.
Back to the metal toes.
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Walk tall in the rain, sleet, AND snow.
No one (wo)man should have all that power.
Just take our money Nike. 💸💸💸
Kick, push, coast this Thursday.
KITH brings the bling.


text:
Batch plot & batch convert application can be used to send multiple Autodesk® Inventor® drawings to different printers convert Inventor documents to a variety of export formats easily & efficiently.
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text:
|Lisa Hicks
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Lisa Hicks
Lisa Hicks is a 16-year veteran of the Competitive Intelligence industry. As a managing partner in Valeo Strategy Group, she delivers premier strategic intelligence and business strategy consulting engagements to the Fortune Global 2000 across a broad range of industries, such as Insurance & Financial Services, Manufacturing/Industrial, Healthcare, Technology & Telecommunications. She excels at providing strategic insights and decision-support to executives looking to expand revenue opportunities and outperform key competitors. She is a respected author and lecturer, and has served on a number of boards, including for the Society of Insurance Research, where she created and chaired their first conference on Health Insurance. Prior to working within the Competitive Intelligence industry, Ms. Hicks worked for 25-years within the Property & Casualty Insurance industry, where she last served on the senior leadership team of ACE INA (now part of Chubb Ins.), one of the largest insurers in the world.
Ms. Hicks has a Bachelor's degree in Finance from the University of South Florida.
9/19/2019Save the Date: SCIP IntelliCon 2020
9/17/2019SCIP - Update


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text:
I was quoted recently in an article published in the U.S. News and World Report. The article was titled, "What You Should Know About Inherited IRAs."
The article had three main points of emphasis.
First, it discussed what happens when a surviving spouse inherits an IRA. Generally, the surviving spouse, for Required Minimum Distribution purposes, can treat the IRA as his or her own IRA and postpone taxable distributions until that surviving spouse reaches the age of 70.5
Second, if a non-spouse is designated as a designated beneficiary of the IRA (typically, a child or children), then the non-spouse beneficiary must start taking distributions the year after the original IRA owner's death. This occurs even if the beneficiary is less than 59.5. The distribution is calculated based on the life expectancy of the beneficiary.
Third, the article addresses how the beneficiary is not bound to the investment decisions made by the original IRA owner. The beneficiary can alter the investments in the IRA or Inherited IRA.


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11:26 PM EDT, Tue September 17, 2019
Feb 8, 2019
MLB.com FastCast: Frank Robinson passes away - 2/7/19
The baseball world mourns the death of MLB Hall of Famer Frank Robinson, plus the Phillies trade for catcher J.T. Realmuto About Major League Baseball: Major League Baseball (MLB) is the most historic professional sports league in the United States and c
That Balls Outta Here 4 hours ago
With the offseason arriving shortly, the Phillies will soon have to decide how to proceed with currently suspended outfielder Odubel Herrera. It has been a long season for the Phillies with many twists and turns along the way. There have been plenty of issues on the field, but the biggest off-the-field issue was the arrest […] Phillies will soon have to revisit Odubel Herrera question - That Balls Outta Here - That Balls Outta Here - A Philadelphia Phillies Fan Site - News, Blogs, Opinion and More
www.thegoodphight.com 4 hours ago
After losing both games they've played in four days, the Phillies are back at it against the... BRAVES?! Ah, yes. The Braves again. As you likely know by now, the Phillies playoff odds have dropped below 1%. Corey Dickerson is out for the season. Adam Haseley is not starting tonight, but Sean Rodriguez and Jose Pirela are. Also, Bryce Harper was ejected from the last game they played. Will he even bother coming back? Ask yourself this... why have you? Tonight's lineup in Atlanta. #RingTheBell : @NBCSPhilly : @SportsRadioWIP : https://t.co/XctKKZzwMg or https://t.co/vgbMhIMsON...
www.thegoodphight.com 7 hours ago
Photo by Mitchell Leff/ There are lots of reasons the Phillies fell short of the postseason this year, but here are my top five. Before y'all get in the comments and point out the obvious — that technically the Phillies are still alive for a playoff berth — let me just acknowledge that you are correct. The Phillies enter their series against Atlanta five games behind the Cubs for the second wild card spot with 14 to play. Mathematically, the Phils have not been eliminated. This is true. It is also true that the Phillies are about to play three games in Atlanta, three games in...
www.thegoodphight.com 9 hours ago
There will be no mention of Dansby Swanson's struggles at the plate | Brad Mills-USA TODAY Sports Discussing the Phillies and Braves with no bias whatsoever Last week, I wrote a preview of the series between the Phillies and the Braves. How did Braves fans like my work? Let's just say they were less than impressed. Here are some of the reactions the article received: Whoever wrote this article needs to be fired, This is awful to read— Braves Ellen (@rgirl661) September 9, 2019 This is awful journalism— Michael (@AlbeeMichael) September 9, 2019 Whatever happens in...
www.thegoodphight.com 11 hours ago
David Kohl-USA TODAY Sports The Good Phil of the Week 9/9-9/15 In one of our biggest celebrations of the Philadelphia Phillies, every week the members of The Good Phight will be issuing one of baseball's greatest achievements to date: The Good Phil of the Week Award. One MLB player and one MiLB player will receive the award each week and will be able to boast to their teammates that they are, in fact, a Good Phil. Major League Good Phil of the Week: Hector Neris Lost in the ridiculousness of the injury-ridden Phillies' 2019 bullpen has been an under-rated and under-appreciated...


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Beautiful real cowhide occasional chair with steel base.
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MicroRNAs regulate several aspects of tumorigenesis and cancer progression. Most cancer tissues are archived formalin-fixed and paraffin-embedded (FFPE). While microRNAs are a more stable form of RNA thought to withstand FFPE-processing and degradation there is only limited evidence for the latter assumption. We examined whether microRNA profiling can be successfully conducted on FFPE cancer tissues using SOLiD ligation based sequencing. Tissue storage times (2–9 years) appeared to not affect the number of detected microRNAs in FFPE samples compared to matched frozen samples (paired t-test p>0.7). Correlations of microRNA expression values were very high across microRNAs in a given sample (Pearson's r = 0.71–0.95). Higher variance of expression values among samples was associated with higher correlation coefficients between FFPE and frozen tissues. One of the FFPE samples in this study was degraded for unknown reasons with a peak read length of 17 nucleotides compared to 21 in all other samples. The number of detected microRNAs in this sample was within the range of microRNAs detected in all other samples. Ligation-based microRNA deep sequencing on FFPE cancer tissues is feasible and RNA degradation to the degree observed in our study appears to not affect the number of microRNAs that can be quantified.
Citation: Meng W, McElroy JP, Volinia S, Palatini J, Warner S, Ayers LW, et al. (2013) Comparison of MicroRNA Deep Sequencing of Matched Formalin-Fixed Paraffin-Embedded and Fresh Frozen Cancer Tissues. PLoS ONE 8(5): e64393. https://doi.org/10.1371/journal.pone.0064393
Editor: Soheil S. Dadras, University of Connecticut Health Center, United States of America
Received: January 2, 2013; Accepted: April 13, 2013; Published: May 16, 2013
Copyright: © 2013 Meng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by funding from the Department of Radiation Oncology and the Comprehensive Cancer Center, and, by the Biostatistics Core and by Microarray shared resource at The Ohio State University. The work was supported by Award Number Grant 8UL1TR000090-05 from the National Center For Advancing Translational Sciences. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center For Advancing Translational Sciences or the National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
MicroRNAs are noncoding small RNAs with a length of 22 to 26 nucleotides (nt). MicroRNAs result in degradation of complementary messenger RNA in many species. MicroRNAs play an important role in cell development, cell death, cell proliferation [1], apoptosis [2] and angiogenesis [3]. The pioneer bead-based flow cytometric microRNA expression profiling method revealed that microRNA profiles reflect the developmental lineage and differentiation of tumors [4]. Normal tissues and cancer tissues were shown to have distinctive expression profiles and microRNA profiles can describe microRNA-driven pathways in solid tumors [5].
The current methods for microRNA quantification are: real-time reverse transcription-PCR [6], [7], microarray [8], Nanostring, and next generation sequencing [9]–[11]. Especially, the newly developed next generation sequencing technologies have the potential to discover novel microRNAs and other small RNAs. Most tissues processed in the health care setting will undergo formalin-fixation and paraffin-embedding (FFPE). Most clinical assays performed in pathology laboratories are optimized for FFPE tissues. FFPE tissues are typically stored at room temperature. Formalin preserves tissue samples by creating cross-linking between proteins, DNA and RNA. DNA extracted from FFPE tissue has been shown to be useful for copy number analysis and mutation analysis on at least one platform in some settings [12]. However, the chemical modification between macromolecules and RNAs caused by formalin fixation can accelerate the degradation of RNA [13] [14]. The stability of microRNAs is generally much more robust than messenger RNA [15]. Next generation sequencing based microRNA expression analysis has been developed on several platforms, including Roche/454 platform, Illumina's Genome Analyzer and ABI's SOLiD platform [16] [17], and different commercial protocols for miRNA library preparation have been developed by the three companies [18]. There is currently only limited evidence available that microRNA sequencing results are reliable and valid. Ma et al. demonstrated the feasibility of miRNA profiling based on Sanger sequencing in 10-year-old archived tissue [19]. To our knowledge there are only two peer-reviewed studies published that compared microRNA sequencing results of matched frozen and FFPE specimens using the Illumina platform [9], [20]. The objective of this study was to determine if FFPE samples can be successfully characterized by deep miRNA sequencing. To this end we analyzed matched frozen and FFPE tissues of different histologies for which detailed processing and storage information was available.
Materials and Methods
Ethics Statement
Human malignant tissue samples procured during period 2003–2009 were obtained from the Cooperative Human Tissue Network (CHTN/NCI), Midwestern Division, which is funded by the National Cancer Institute, based on an internal review board (IRB) approved research protocol (Ohio State University Human Subjects Protocol - 2011E0377). Other Investigators may have received specimens from the same subjects.
Tissue specimens
Eight paired human malignant tissue samples were received. Remnant surgical tissues were taken after diagnostic samples were secured from patients (five males and four females; median age 58 years, range 39–78 years) with breast invasive ductal carcinoma (2), renal clear cell carcinoma (2), lung adenocarcinoma (1), prostate adenocarcinoma (1), metastatic melanoma (1), and sarcoma of thigh (1). Surgical specimens were transported from the operating rooms to Tissue Procurement, examined under a pathologist's supervision and preserved within 5 to 57 recorded minutes. Paired tissue samples selected for research were either immediately snap frozen in liquid nitrogen and placed in a –80°C freezer for monitored storage or fixed in 10% buffered formalin for up to 24 hours and then processed into a paraffin embedded block and stored at room temperature. When the research samples were received from CHTN, the coded samples were accompanied by a coded final pathology report and a quality assessment report verifying collection of tumor tissue. The specimens have been reviewed by a pathologist and 4 cases had 100% tumor, 3 cases 90–95% and 1 case 50–60% tumor, and matched FFPE and frozen samples were generally comparable in tumor content and size. The clinicopathologic features of the cases used for microRNA sequencing and expression analysis are listed in table 1.
RNA Isolation, quantitation, and quality Assessment
RNA from FFPE samples were extracted using Recover All Total Nucleic Acid Isolation Kit (Life Technologies Corporation, Carlsbad, CA). Briefly, 5–10 mg samples were sliced from paraffin blocks and deparaffinizated by xylene at 50°C, followed by 100% ethanol wash. The air-dry tissue samples were digested by proteinase K for 24 hrs in a microtube shaking incubator set at 50°C. The digested samples were mixed with appropriate volume of isolation additive and 100% ethanol. After passing the mixture through the filter cartridge, the DNA and RNA were retained on the filter. The DNA was removed by on-filter DNase digestion. The RNA was purified by washing buffer and eluted with nuclease-free water.
The microRNA samples from the fresh frozen samples were extracted using PureLink™ miRNA Isolation Kit (Life Technologies Corporation, Carlsbad, CA). Briefly, 5 mg fresh frozen tissue samples were mixed with 300 µl Binding Buffer (L3) and homogenized using tissue homogenizer. The homogenized samples were centrifuged, and supernatant were mixed with 300 µl 70% ethanol. The solution containing the RNA was purified by two round of spin columns cleanup. The RNA was eluted with 50–100 µl sterile RNase-free water.
MicroRNA sequencing
Small RNAs from FFPE and fresh frozen samples were prepared for SOLiD sequencing as follows: the total RNA samples were processed by the flashPAGE fractionator (Ambion) and flashPAGE Clean-Up Kit (Ambion). The enriched small RNA was then processed according to the SOLiD Small RNA Expression Kit protocol (Applied Biosystems). The purified small RNAs were ligated with 5' and 3' adapter mix using RNA ligase. The ligated products (40–60 bases in length) were reverse transcribed and purified on Novex 10% TBE-Urea gel. Subsequently, 15–18 cycles of PCR were performed by amplifying the purified cDNA with barcoded PCR primer sets provided in the kit, which differed by a unique 6-nucleotide sequence. The amplified products were loaded on Novex 6% TBE gel (Invitrogen) and the gel bands containing 110 to 130 bp fragments were excised. The amplified products were purfied from excised gel band, amplified by emulsion PCR, then loaded on Applied Biosystems SOLiD 4 next generation high throughput sequencing system for data acquisition. The quality of the samples and libraries were verified on the Agilent Bioanalyzer [21], [22]. The raw SOLiD sequencing data were uploaded to the NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE45740 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE45740).
RT-qPCR for microRNA validation
The RNA from fresh frozen and FFPE tissues were validated by real-time (RT) quantitative PCR. microRNA expression was normalized to the small nuclear RNA U6 using TaqMan MicroRNA Assay kits (Applied Biosystems, Darmstadt, Germany). A 30 ng sample of RNA was processed by the TaqMan MicroRNA Reverse Transcription kit (Applied Biosystems, Darmstadt, Germany). In brief, 5 µl RNA was mixed with 1 mmol/l of each deoxyribonucleotide triphosphate, 50 units of Multiscribe Reverse Transcriptase, 5× reaction buffers, 4 units RNase inhibitor, and 5× gene-specific RT primers mix in a final reaction volume of 15 µl. Reactions were then incubated at 16°C for 30 min, 42°C for 30 min, 85°C for 15 min with a final hold at 4°C. Afterward, 15 µl of the cDNA solution was diluted by nuclease-free water to a final volume of 100 µl. Quantitive PCR was run on a 7900 HT PCR machine with denaturation step at 95°C for 10 minutes, followed by 40 cycles of a denaturation step at 95°C for 15 seconds, and an annealing/elongation step at 60°C for 60 seconds. Fold change for microRNA in tissue samples was then calculated using the equation 2-ΔCt test/2-ΔCt control.
Data analysis
The linker sequences of small RNA libraries were removed using cutadapt software (http://code.google.com/p/cutadapt/) under default parameters. The length distribution of microRNA was constructed by plotting reads counts against 0–30 nucleotide lengths. The small RNA Analysis Pipeline Tool from Applied Biosystems was used with the following parameters to include only sufficiently high quality reads in our study: 1) Minimal average read quality threshold was 20 (The QV value is calculated using a phred like score q = –10× log10 (p) where q is the quality value and (p) is the predicted probability that the color call is incorrect.), 2) to be stringent we allowed only 1 mismatch between reads and miRBase version 16 hits for each counted read 3) the minimal length of aligned reads had to be 17 bases. Data were normalized as reads per million (RPM). Sequences with expression value below 5 RPMs were considered not detected for the correlation between frozen and FFPE analysis part.
Effects of FFPE sample degradation: microRNA read length distribution
Formalin fixation and long storage times are known to result in RNA degradation [23]. This degradation results in a shorter average RNA length [24]. To identify possible degradation effects of microRNAs, we analyzed the read length distribution of matched fresh frozen and FFPE cancer tissue samples. After the 3' adaptor sequences of each read were trimmed, sequence reads length distribution was examined (figure 1). Read lengths were observed to be centered at 21 nt in all frozen samples and all but one FFPE samples. There was one FFPE sample whose peak microRNA length was found at 17 nt, indicating significant degradation. We then carefully examined the small RNA classification profiles and correlation of microRNA expression values of fresh frozen and FFPE samples. We found that there are negligible or no effects on those analyses. Storage times or preparation methods were not different between this degraded FFPE sample and the other samples analyzed in this study. Also, the proportion of small RNAs in the 20–22 nt range was lower for several cases in the FFPE compared to the matched frozen specimens (one renal cell carcinoma case, the lung adenocarcinoma case, and the prostate cancer case). This is also likely due to degradation in FFPE samples, although small variations during the library preparation could partially contribute to the observed differences.
Effects of FFPE sample degradation: storage time
The storage time of the samples ranged from 2 to 9 years. 624 micoRNAs and their precursors were identified in the 8 pairs of tissue samples. Some microRNAs could not be detected in either fresh frozen or FFPE samples. Figure 2 shows the percentages of microRNAs that were detected in both fresh frozen and FFPE samples, only in fresh frozen samples and only in FFPE samples over the year of specimen collection. There were no storage-time-dependent changes in those percentages, indicating that there is no significant association between storage time and the number of microRNAs present during this 9 year time window.
We next examined the percentage of microRNA reads among total reads (figure 3) to understand if the purity of microRNAs was reduced in the FFPE samples. Approximately 70% and 85% of reads in FFPE and frozen samples, respectively, were identified as microRNA sequences. A possible reason for this difference could be the presence of fragments of lncRNAs and mRNAs in the fraction of RNA smaller than 40 nucleotides (nt) in FFPE tissues (figure 4).
Effects of FFPE sample degradation: classification of small RNAs
To better understand if the difference in the percentage of small RNA sequences among all reads between frozen and FFPE tissue data we examined the distribution of RNA classes in one of the breast cancer sample. There were a total of 11563414 and 10273837 sequencing reads, in the two examined matched samples and the reads were mapped to the human genome (hg19, National Center of biotechnology information building) using custom Python scripts. The reads were classified in different RNA groups according to the EnsEMBL database. The majority of small RNAs in both fresh frozen and FFPE libraries were microRNAs (figure 4). Comparing data from matched fresh frozen samples to data from FFPE samples there were lincRNA and sequences of unknown classes in data obtained from FFPE samples. Given that only RNAs smaller than 40 nt were extracted and processed for sequencing this suggests that there is fragmentation of lincRNA and other long RNA in FFPE tissues, which are longer than 40 nt.
A total of 469 to 548 (mean 519) different microRNAs and their precursors were identified in both fresh frozen and FFPE samples. We determined the percentages of microRNAs that have more than a 2 fold difference of expression between matched fresh frozen and FFPE samples (figure 5). Among the different cases, 69 to 145 microRNA transcripts were more than 2 fold overexpressed in fresh frozen samples. 73 to 186 microRNA transcripts were more than 2 fold overexpressed in FFPE tissues (figure 5).
Correlation of microRNA expression values of matched FFPE and frozen samples
We next determined the correlation of microRNA expression values of matched FFPE samples and fresh frozen samples. There were 250 microRNAs detected in all samples in this study. There was a strong correlation of microRNA expression values in matched FFPE and snap fresh frozen tissues across all microRNAs and all cases (r = 0.85; figure 6). Correlations across detected microRNAs from the same tumor/patient were high, with r ranging from 0.71–0.95 (figure 7) [25].
Only the 250 miRs with no missing data were used.
Each dot represents the expression values of one microRNA in a fresh frozen-FFPE pair.
The mean of Pearson correlation coefficients of individual microRNA expression values of FFPE and frozen tissues was 0.46 (figure 8), with only 41 mature microRNAs and precursors having correlation coefficients higher than 0.8 (table 2). We sought out to identify features that are associated with higher correlation coefficients indicating invariance to the tissue preservation method. To test if the level of expression of a given microRNA is associated with the correlation between fresh frozen and FFPE tissue, Fisher transformed (arctanh) correlations were regressed onto mean FFPE expression (figure 9). There was no association between expression values and correlations between data from frozen and FFPE tissue observed. We then sought out to understand if a possible lack of variation of individual microRNAs among the biological replicates is resulting in poor correlations. Fisher transformed correlations were regressed onto log transformed FFPE variances. The variance analysis showed that microRNAs with higher variance have significantly higher correlations of data from FFPE and frozen tissues (figure 10; arctanh(r) = 0.47+0.59×log10 (FFPE variance)), which may be due to the presence of true biological variation present in these microRNAs.
PCR validation of microRNA expression
We selected two microRNAs (miR-21 and miR-19a) that were expressed higher in frozen samples than in FFPE samples in our microRNA sequencing results for validation using PCR (Taqman). Both miR-21 and miR-19a were found to also be higher expressed in frozen tissue than in matched FFPE samples using a PCR assay (figure 11), consistent with our sequencing results.
RNA samples from fresh frozen (n = 8) and FFPE (n = 8) tissues were analyzed. Small nuclear RNA U6 was used as reference. MiR-21 and miR-19a had higher expression in fresh frozen samples than in FFPE samples, consistent with sequencing results.
MicroRNAs as gene expression regulators appear to have an important role in cancer cell differentiation [4], proliferation [26], metastasis [27] and apoptosis [28]. There have been many microRNA profiling efforts using frozen cancer tissues [29] [30] [31]. Cancer tissue specimens are typically archived after formalin fixation and paraffin embedding (FFPE) and frozen tissue is only available for a minority of cases in pathology archives and tissue banks. A better understanding of the quality of microRNA sequencing results of FFPE tissues would allow for a more informed decision as to when use of FFPE tissue for small RNA sequencing experiments would be appropriate.
In this study, we analyzed small RNA sequencing profiles of eight pairs of matched fresh frozen and FFPE cancer tissue samples using the ABI SOLiD 4 platform. Storage time up to nine years appeared to not affect the number of detectable microRNAs in either frozen of FFPE tissue in this study. The presence of higher percentages of non-microRNAs in the fraction of nucleotides smaller than 40 nt suggested that FFPE tissues are more degraded, though. However, this apparent degradation did not result in fewer microRNAs being detected in FFPE tissues, suggesting that a sequencing approach can overcome sample degradation to some degree. While small RNAs in one of eight FFPE samples appeared degraded as suggested by the reduced read lengths centered at 17 nt compared to the other eight FFPE and all eight frozen samples centered at 21 nt, the number of aligned reads and the number of detected microRNAs in this degraded sample were similar to the data from other samples, which suggests that data obtained from samples with the above described degree of degradation would not necessarily need to be removed from all types of analysis.
Overall, there was excellent correlation between miRNA expression values between matched frozen and FFPE when combining all samples and all microRNAs (fig. 6), or when analyzing all microRNAs within individual patient sample pairs (fig. 7). Those findings are consisted with previous reports using different sequencing platforms suggesting that sequencing approaches can result in good correlations between FFPE and frozen samples when including several hundred microRNAs [32] [33] [9] [34].
Correlations of expression values of individual microRNAs (see figs. 8, 9, 10) were lower than correlations including all microRNAs (figs 6, 7). The underlying reason for this observation likely originates in the identified association of correlation coefficients of individual microRNAs and variance of microRNA expression values (fig 10). Very similar expression values of an individual microRNA among the eight cases (equal to low variance in fig. 10) typically result in low correlation coefficients because the variation between frozen and FFPE data is larger than the variation of expression between the eight cases included in this study. Therefore it is not necessarily surprising to have a wide distribution of correlations of individual microRNAs as analyzed in detail in figures 8, 9, and 10.
Limitations of this analysis include limited small sample size and potential PCR drifts and PCR selection biases during amplification in the multiplex PCR based library construction [35] [36]. This disadvantage should be overcome by the 3rd generation of sequencing technology, which is thought to not require amplification of the target sequence anymore but detect single nucleic acid molecules [37]. Furthermore, the use of adjacent tissue to create matched specimens for this analysis introduces some tissue heterogeneity effects and likely contributes to the observed differences between matched frozen and FFPE samples. In summary, microRNA sequencing of FFPE tissues is feasible using ligation-based sequencing and microRNA expression values of individual patients are highly correlated with expression data from matched frozen tissues. MicroRNAs with higher variance appear to have a higher correlation of their expression values in matched frozen and FFPE tissue data.
Author Contributions
Conceived and designed the experiments: WM TL. Performed the experiments: WM JP SW. Analyzed the data: WM JPM SV TL. Contributed reagents/materials/analysis tools: JPM SV JP LWA KP AC TL. Wrote the paper: WM JPM LWA TL.
- 1. Ambros V (2004) The functions of animal microRNAs. Nature 431: 350–355.
- 2. Chang TC, Wentzel EA, Kent OA, Ramachandran K, Mullendore M, et al. (2007) Transactivation of miR-34a by p53 broadly influences gene expression and promotes apoptosis. Mol Cell 26: 745–752.
- 3. Dews M, Homayouni A, Yu D, Murphy D, Sevignani C, et al. (2006) Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster. Nat Genet 38: 1060–1065.
- 4. Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, et al. (2005) MicroRNA expression profiles classify human cancers. Nature 435: 834–838.
- 5. Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, et al. (2006) A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci U S A 103: 2257–2261.
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Day: August 28, 2018
DOST, UPD target Oct. 29 as launch from Japan of Diwata-2, 2nd PH-owned satellite
Diwata-2 (in photo), 'sibling' of Diwata-1, will soon orbit Earth with Oct. 29 as target launch date from Japan.
Update: Holy cow: 2 super lucky bettors win Php1.18-B huge Ultra Lotto jackpot!
CELEBRATE! Finally, two billion-peso lucky bettors won the massive jackpot of Ultra Lotto 6/58 on Sunday, October 14. The prize, a super cool Php1.18 billion! Congratulations to the two winners. The Philippine Charity Sweepstakes Office (PCSO) announced the winning numbers — 40-50-37-25-01-45 — on its website. The two winners purchased their winning tickets at lotto […]


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News this month that the number of whales discovered entangled off the West Coast had actually reduced in 2017 motivated optimism amongst some. But, currently initial numbers for 2018 are headed back toward the record highs of simply a couple of years ago.
While whale entanglements in UNITED STATE waters were somewhat over the 10-year standard in 2017, the National Oceanic as well as Atmospheric Administration reported on Dec. 6 that West Shore numbers were virtually half the 2015 and also 2016 stats. Of the 31 entangles whales reported in 2017, 25 remained in the waters off The golden state– humpbacks that such as to eat anchovies in the main coast areas fished for crab and prawn blazed a trail, but gray whales were stone's throw behind. NOAA's initial 2018 numbers report 45 knotted whales validated in the waters off Alaska, Oregon, Washington and also The golden state; 35 of which were found off The golden state. Much of the battling whales have actually been spotted off Orange Area and also Monterey — 2 areas that government officials say are bustling with seafarers, fishing and whales. Final numbers are expected in March.
"It's promising to see the numbers in 2017 reduced," Justin Greenman, California assistant stranding coordinator at NOAA, stated. "But it does not mean the issue has actually reduced or there has actually been a change in risk. If whales are not near the shore, we will not see them."
Professionals say the fluctuations can be clarified by a number of elements– consisting of that there are simply a lot more whales around which sea environment changes are leading them to alter where they quit to feed. From 2013 to 2016, "warm ball" ocean conditions– when water temperatures rose– forced marine mammals to look for food close to the coastline, enhancing their risk of ending up being entangled in traps.
However we also may just be counting the variety of incidents much better, authorities claimed, due to the fact that of tipped up efforts by whale watch charters, private boaters and also citizen scientists over the last decade to end up being a lot more informed on complexity hazards as well as exactly how to report them.
From 2000 to 2012, approximately eight whales a year were reported knotted on the West Shore. Yet complexities "have actually taken place for decades," claimed Peter Folkens, that is one of 3 in The golden state who leads rescues for NOAA's West Shore Big Whale Complexity Action Network. "It's a better recognition that's affecting the greater numbers we're finding.
"Over the years, it was primarily unintentional explorations," he stated. "As individuals become much better informed regarding the relevance of coverage, the number of reports increases."
Because 2013, recording complications has actually dramatically enhanced.
Numerous whale watch charters run out of Southern The golden state harbors, as well as due to passion for the aquatic mammals and in the passion of their resources, there has actually been a desire amongst the boat captains to join the effort to aid the entangled animals. Real-time as well as precise coverage has actually increased with their added eyes on the water as well as the use of photography as well as drones.
Chris Pica, a captain at Dana Wharf Whale Enjoying, who has actually gotten on the water for three decades, was educated by NOAA in 2010 and has actually because assisted in disentanglement occasions.
"When you see an animal as majestic as a whale entangled, it's really disturbing," the San Clemente captain claimed. "There's a lot even more information around currently, also amongst personal sailors. Every person is encouraged to aid."
The federal agency recently published indicators near the boat launch ramps in Dana Point Harbor concerning what to do if an entangled whale is found.


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16+ list motywacyjny bez doświadczenia na danym stanowisku
Every sort of letter includes. There are numerous sorts of consent letter. Thus, to continue to continue to keep your correspondence plausible, you must give proof. Should possess a investigation note.
In the event you find it possible to type this correspondence. The correspondence might also be called a reference correspondence. Resume letter A version job request letter has to have the objective of providing a exact excellent impression of its creator therefore he could procure a project that is particular.
You might need to address every letter along with customize. You will need to know that the candidates well to be in a position expressing their proper personality Foryou to write decent reference letters. A reference letter describes to the individual's essence is much more widespread in nature, and it isn't dealt with to anyone specifically. A reference letter which is written defectively could lead into the student never or not obtaining a scholarship obtaining a scholarship whatsoever in virtually any way. A character reference correspondence (also at times known as a letter of private reference or personal suggestion ) is employed to recommend some one for a situation in an organization or for some capacity in an company. If a individual who you're uneasy with asks you to write them a character reference correspondence or whether you can't think of enough fantastic things to express regarding the individual, then it is going to become better if you will locate a method to escape from writing it. Simply stick to this 6steps and also you are able to compose a character reference letter free of moment!
Letters should prevent talking to delicate own matters, simply because they might be read by folks. Thus dependent on the rationale one needs to compose the correspondence. All of you'll require is a simple letter. At an identical point that you can write a generic onetime endorsement letter that may be used for many unique sources, it really is more effective, and more personal, to compose.
You might need to create 1 letter or template that may be individualized for each college. Keep in mind that the correspondence should be prepared in a mode that the prospect is employed, If writing such document for all those. You may even get exclusive top thoughts and sample letters to help you. You will be more surprised at how fast you might soon be able posture to transform this sample correspondence in to a personalized and unique one. Once you search for sample private letters of recommendation, be sure to tailor to fulfill your needs.
Telephone at the business In the event you don't recognize the title of the person and ask that the title. You can set your institution's title or the name of organisation, Rather than the personal name. You may possibly just place your identify, however If it truly is inter-departmental, it's prudent to always place your very original and last name. Then , you just mention that the authoras last name in the event you use the foundation.
That you don't want to produce a letter for each college, As you are definitely going to really be seeking advice from numerous diverse colleges. The details you send out may help me learn more making an educated decision regarding my future and regarding your college. You must obtain information. It is highly advisable to add information that permits the worker to cover the thing further should it's necessary. In the decision of your correspondence, you also encourage the scholarship committee to acquire in connection with some further concerns and also will present your contact info. Just be certain you verify that you've got the proper contact details all.
A superior example of one can be found beneath. Their instances would have to be expanded over within the paragraph for a means to make them convincing. Zero cases will likely be excellent, so take a look at the others out there. In order to evaluate something, you want to evaluate it with the ideal example of this item.
Students loved Ms. Dominguez due to her nurturing personality. You can find lots of pupils who hardly understand when now is the time and energy to write student letters the way to inquire. The better part of the momentthey are requested to compose debate papers which present a particular point of perspective and make an effort to convince your viewer. You must be in a position to confirm all the student's qualifications in detail. Maybe you would want to recommend students or worker or would like to make a program for a instructional app.
An example correspondence is valuable in the proceedings when you are attempting to find function, you're at your wit's end about ways to approach a possible employer. It is offered in the content under. Be certain that you do not compose a letter to some one else you may want to ask a favor, as it can be viewed as impolite and inconsiderate.
Your correspondence should become direct. In paragraph , spell out what the letter is meant to pay for. The term permission letter is likewise utilized to symbolize a letter that can be being written to enable an individual consent to do a particular occupation, for instance, selecting a fieldtrip. For writing a consent letter, the primary reason is always to discover an individu endorsement that you have to acquire. When you write a consent letter, then be certain you sign it and maintain a duplicate of the letter along with yourself to avoid any form of misunderstanding later on. A letter giving someone permission to take care of your small one may save a good deal of hassle and injury when you want somebody to select him up from school, get him into health related conditions or behave because the most important caregiver for more than a handful of hours.
Make certain that the hand writing is clear and legible if you're writing the letter in place of studying. Utilize bullet tips when possible to produce the correspondence a lot much easier to browse, and plainly say what actions you are interested in getting the outsider to take your behalf, such as requesting he or she sign a particular bill into lawenforcement. For authorizing somebody to perform your authorized, company, health, and other trades for your own benefit authorization letters have been wanted. Writing a business letter is a skill for everyone else who would love persuasive and to work. It is easily recognizable by its structure and style. It might be helpful to know that a simple template should be adhered to by each of organization letters, if writing a small business letter appears daunting. Although it could appear to become quite a normal small business correspondence that is small, ability letters possess a excellent deal of valid implications.
Each and every once in a while, writing your pal and enabling every one understand what is with you. Keep. It truly is uniform for each one of the letters which belong into a specific correspondence. The appeal correspondence needs to be composed as an easy means to gain the circumstance foryou personally. In the current world of emails that are casual, understanding how to format a expert letter is crucial. Letters that are indented take a closing that is focused, even though a block letter format closing needs to keep left-justified. It must be a clear correspondence together with particulars.
You really do not know, your letter could get the change you want. You first must understand what precisely you must add from this letter In order be able to understand what a apology correspondence appears . The trick to creating a great letter asking for a donation is to understand to manage which likely contributor. Therefore be careful when letters are drafted by you. The key to producing a suggestion is always to stay glued to this ideal structure, which is explained in such methods. Therefore now you're aware of how to get started creating a letter. Most sales letters possess a tendency to search out their means to the dustbin.
You will find some items to think about while writing a grievance letter. An individual should not send'kind letters', since they are most likely to receive ignored. It is fairly simple to draft a letter of intent especially in the event that you've obtained the arrangement at hands that may possibly work as a guide. As a way to stay, it's clear , letters are all here. When you wish to share with your premium in regards to the abandon of absence A letter of application to leave needs to be written you prefer to simply take.
An individual ought to keep in mind that there are essentially just two formats for correspondence writing, to begin. The correspondence format has to be proper. A company letter arrangement is used if the receiver is exceptionally regarded or while the writer does not understand the average person and therefore is needed. Shifting formats in just a correspondence can cause you to be seen as someone who will not know fundamentals of communication. It's crucial that you realize the format of the letter before you get started producing 1. This letter's format stays exactly the same. Make sure you really own the date arrangement for the recipient.
You have to get the following upcoming steps, to acquire an occupation as an accountant. Management Accountant and cost can commence earning at a rather young age. Becoming a professional necessitates exactly the proper mindset and also techniques. When you have been into an accountant earlier or you simply require a refresher, here's an extensive selection of records that you need to bring to take advantage of your own trip. It's understandable that whether it is an undeniable simple fact at all respects a consultant, an accountant will be, the alternative is not always exact. Be certain you work with a tax accountant who understands your market and company model which means you're working from probably the arrangement and maximising of the tax incentives and deductions that are readily available for your requirements personally. Tax attorneys wish to get to the w3 and also W-2 kinds for the organization.
Accounting can be you're likely to escape from school and also be in a posture and a increasingly escalating subject. When you proceed into intermediate and more complex bookkeeping, they accurately depict what's going on for even a firm or a business, you learn to listing things. Therefore, accounting is an organized and exhaustive understanding of business trades. Bank harmony bookkeeping has become the standard and most primitive type of accounting.
Being an accountant now is only as much creative since it truly is technical. It's not simple to decipher the tests successfully, so enough moment; point. You most likely have no a lot of time for you to invest in your own BPs to begin with. That is perhaps maybe not that the close of the story if you are delighted about work. The job of Price Accounting is by how the business' sustainability could be optimised, also to track down the amount of generation, the amount. The career of finishing yields is becoming more and more automated. Whether youleaving college or're altering your career, building a livelihood in accounting requires an suitable qualification to be received by you.
The amounts which you put in each account are dictated according to your revenue. An account should keep carefully the adoption of guidelines in order to pull on the maximum lucrative summary lawfully. Whenever you are asking to get money for the company however big the transaction is, you want to offer you a BP earlier or later after.
The procedure to become C.A. is definitely an incredibly hard. You'd be given of the documents that were hard copy, and you would certainly do a lot of data entry, making certain that the account more balanced and reconciled, and also searching for glitches in accounting. Bank invoices , charge card statement and mortgage announcements, especially for the month of this calendar year, are most often helpful. Report on expenditures, revenue, assets, and liabilities needs to be created. You must get acquainted with statements. It truly is quite necessary and common so as to build metrics and actionable economic statements.
As a way to make your organization it helps to seek the services of a seasoned accounting and fund pro. The company is easily modified the surroundings in light of their specifications. It makes certain that they are respected and provides centers. There are about 2000 games businesses in the uk at the present time.
An organization depends on efficacy of this direction to direct an company or business to find a leap of achievement. It's never been quicker to set a company enterprise or simpler. Sure it is not difficult to stuff something up, if you receive a business that is very simple. You are getting to have range of services and products available to market if you're like firm.
QuickBooks on the web presently includes your transactions that are applicable. You might watch or look out for career openings on line to create your work hunt less complicated. With fiscal data generated on your organization and this company, it really is simple to become overrun with all information's amount. An accountant's job description has changed through the years too.
Perhaps not an enjoyable topic however you must understand the theory supporting your graph of account if you would like to truly know the health of one's SaaS enterprise and employ the right structure. Focusing with the BP can be exercice to carry up decision making and your strategy and really a good tool. An outstanding financial plan will reveal your potential. It is essential to become more obsessed along from every single account with your spending it defeats the purpose plus also you established a great deal of admin of benefit. Payroll expenditures Payroll expenditures are often a huge expense for companies. It's mandatory that you get prepared for that calculation that is great till you have facility for the right accounting technique because it is hard for anybody. Now it is very simple to produce the accounting calculation easier and that is potential via accounting system and the finest.
Ensure you're clear on what your contract claims, particularly if you are expecting to find total use rights. It is possible to purchase the contracts . Make certain the word-choice is clear and consistent through the duration of the agreement that is complete. A contract is supposed to be more legal if it is accepted by the court of law. Lots of photography's Sample exemptions was cited here. Nonetheless, it will function as a fantastic sample images contract to secure you started.
There is a different type of agreement known as a shopping agreement that a much improved alternate to a substitute agreement free from other provisions, or buy provision. Of course far a lot more terms will be contained by an agreement. Such as I've said often situations, the agreement has to become knowable. The portrait agreement functions as the foundation to get a wonderful photography companies presented and tips the entire business trade.
The arrangement will probably comprise all the stipulations of the deal that will to be redeemed. In the event you decide you need to find all seven of the very usually used images contracts from me and want to buy, it's merely $15 to find the deal. The images Contract helps make it simpler to get paid punctually and in full. Images Contracts comprise the basics. The ideal method to accomplish so is to learn what ought to be in your wedding ceremony photography arrangement.
Planning a meeting takes plenty of work. Just enjoy when you compose and designing a restart, make sure that your suggestion isn't hard to read to your client. Possessing a template is probably going to make suggestions for you. You can find many legal considerations and a specialist photographer will understand regarding these. The aim of producing a contract will be always to get rid of the adverse negative impacts that might occur in future.
Every provider takes over 1 record to fasten its pursuits. Whether you are just setting up a photography provider, or have been struggling with it for a reach of years, I want to ask you a question. Independent Contractor contract In case you're providing solutions for a huge occasion or even perhaps a long event, there may be occasions in which you have to employ Separate Contractor or a freelance to help you. Hone your services your expertise and also the way you market yourself.
It is a system of setting out what you are very likely to accomplish to your consumer. Together with the addition of a process and landmark department, your customer wont wonder at which you are in the procedure nor will micro-manage you. You can not show such a thing sometimes simply concealing the identify of the customer is sufficient.
You can find assorted sorts of photographer contract template for specific purposes. The 2 nd key part in this photographer deal template is that the body. Remember to be mindful that if you decide to employ a template, you ought to think about having the agreement reviewed by legal counsel. Ideal issue concerning this template is you can easily change its contents and factors based on your own wants with Microsoft word onto your own personal computer that is personal. First select the template that you pick. Need less to state there are lots of free contract templates online.
The Photographer shall not be accountable for photographs never taken being a effect of this customer's failure to furnish cooperation or support. Whether you are a photographer or consumer, then our agency template that is photography that is ready-made may probably supply an ideal procedure to fill out the deal to you. Images has lots of moral grey areas. Portrait images can be perhaps a set of women and men at many regions or in just an image studio or actually a shooting picture of someone.
You need to know or know the ideal method to make considered described as a photographer. Photographers can utilize the sample to get helpful information or being a template. When selecting a wedding photographer, bear in mind that you're hunting for a professional. Photographers aren't predicted to find persons in public locations model releases. didn't mean he wouldn't have now been detected out from the sneering on line audience although Even the photographer foolishly outed himself. You will be supplied by A good photographer with methods to take pleasure in your system form and ways to stand. If you are a little school photographer attempting to raise your college portrait provider, you need to keep in mind it's just as important to retain a school once you reserve them when it is always to reserve organization.
Your school ought to really have a livelihood center where you are able to have interviews or else you could ask a friend to assist you practice. That you really don't want to look just like you peaked at senior school. Personal universities will typically require additional time together with pursuits from teachers away from the school day. Since they do not have a basketball crew middle schools don't need a basketball trainer. You might be a new senior high school or university grad, or you might be going into the workforce to the moment.
People which aren't certified and individuals who are certified can become substitute teachers. To obtain extra income, then a school teacher may choose to function in an administrative section together with a teaching job or eventually end up functioning elsewhere. Private school teachers typically have the job as public school educators, but they're not often expected to receive any certification. Charter schools have no marriages.
Maybe not merely are you able to develop a few sorts of expertise but you are demonstrating potential employers that you're an man ready to dedicate themselves. It is helpful to add skills which you have on a restart. You will learn several of the specific knowledge at both regions.
In return, you have got a internship expertise which is able to allow you to land an opportunity later on. Or, even in case you have no work knowledge, list your skills and just how you got them. You also has to demonstrate your power to succeed at your interview at the job via your performance Since you don't have a job experience. At times, you may well not possess the operate experiences that are relevant with the task which you're applying for.
You most likely know it's most effective to render your overall address off your restart if you prefer to re locate to get work. In the event you detect work being an internet researcher, then you may be researching from attempting to locate online advice for law firms to accomplishing the legwork for college or university sections. Have one minute to think of any problems you solved in the office. You will be more restricted as you won't be in a situation to control power saws, although you can conduct work.
Waiting to truly own a project will far a lot more often than not find yourself in a glitch earlier or later later. TAKE IT, if you are able to locate work that enable one to be to a CEO. It's not vital to record each job that you've held before, specially if it's not applicable. You may be looking for internship or a summer job, or a scholarship or faculty app takes you to add a resume.
You might apply for a job where they has been for some one to upgrade the organization website using pictures however doesn't have the opportunity to accomplish himself. Match your abilities to this company or occupation you are looking for. Do the job done Make certain that to can DO your job. You may possibly have eventually discovered the occupation.
You will understand exactlywhat the job ought to be to demonstrate that you are the right match, by acquiring a fantastic understanding of stuff you are going to do everyday at work. Chancing upon a job does not need to be so hard, it truly doesn't. Nevertheless old you are, having the job can be a hard experience. Listing your chances would be your thing to do although volunteer gig or your very first job may have now been years ago.
Using the popularity of basketball along with with all the requirement for basketball trainers, locating a training position might not be that difficult. Teaching positions are alternatives for college pupils who do not mind a tiny bit of paperwork and enjoy working with people that are distinct. How disappointing it's to be denied as a effect of the little misprint in job standing!
There's no inch size fits if it regards resumes. Your resume has an aim rather than an expert overview Objective statements describe your demands, in place of how you will meet an employer's necessities. Resumes, especially for teens, must be about a single page just. They have traditionally become the primary ticket to surfing the work market. Eventually, everything in your own resume must promote its own relevancy and readability.
An cloud invoicing option enables one to store all. By the viewpoint of buyer or the consumer, invoicing isn't just a process. Invoicing is on the cloud which gives the capacity to take care of billing and client info from inch application to you.
Invoices are utilized by associations with people to keep up a listing of transactions made in business in addition to in personal daily life. Cretica bill includes. During the assistance of a bill template you can make an invoice that's simple to read and understand and that's going to meet your needs. All round Invoice enables you keep detailed records of your clients' titles also. When potential consumers desire additional funds sources for their job A proforma statement is remarkably useful. Industrial invoice is a file. At case of a customs inspection, a well prepared small business invoice can support the customs officer require a fast decision concerning how your dispatch should be successfully processed.
Anonymous newspaper makes clients worried concerning your organization's legitimacy. Carbon newspaper contains some uses that are exceptional. There are 3 sheets. By employing the Microsoft phrases it really is true , you can cause the fax cover sheets according to the needs of one's company. As it has to do with selecting a facsimile cover sheet for your company organization, then you might be many fax cover sheets that are freely on the web to download. Even with demand out of tens of thousands of companies, solitary Invoice protect is currently working over a scale for explanations that are assorted.
You may down load a template and fill it outside with the advice related to a main act to start. The templates in our future department will help relieve a number of their economic stress which comes with a buy. It follows. Templates could be received by you . Generally speaking it's amazing to have a template ready to be more full of if you aren't prepared it could turn into a more significant headache of course because you never ever understand what job will inquire to these. There is A statement template merely a template in which details regarding an item may be filled to turn it into an invoice. Down load and complete a sterile statement template to ship into client or a customer to be able to amass form a service or solution rendered.
It is easy to modify by creating brand new columns to monitor extra info. You could use the normal templates from the excel. You can find several such invoice templates it-as relatively straightforward to produce your own personal along with available.
The following down load on exactly the exact page is the Excel file that will help for your projections. A invoicing program could be your approach to go. qualities you might require are comprised by the system. The process typically involves controlling the sam e together with sheets and pulling on data from systems. Before the actual record is created, it begins. It is maybe not given much importance or has been neglected that subsequently translates into lack of collaboration between your stakeholders. The below mentioned Excel format files have been provided to make recognition.
About almost any small business' spine ends, the consumer is currently advised Being a effect of the planet wide web. You'll need to Unpost it to track down a invoice, use the Business Client discover menu, but before it's potential to edit a submitted invoice. Simply edit the template reveal exactly the HST level, In case your business can be found in a province that uses the Harmonized Sales Tax. The firm can make the adjustments that are critical to create some thing which genuinely meets people's needs. If your business is sufficiently virtual utilize travel for an opportunity. Do the same and observe what's happening indoors fiscally.
You should do to warrant your prices. You may not be certain what your pricing is very likely to search just like at a few months. In case , you feel you are bidding at a top price in comparison it really is ideal to justify the reason. To recognize your reply you must recognize the price tag. In addition, there would be the expenditures associated for stamp, ink, paper and newspaper. You also could need to accomplish the same as a way to boost your business finances. You're able to download a high quality blank work order to create your operate orders that are great looking .
You need to get an agenda of action to allow you to accomplish your aims, when you'd really like to track down a job. Jobs can be located in numerous ways like walking and asking if there exists an organization choosing even the web or the neighborhood paper. It is the the task of the nurse to be certain the affected person makes a recovery, after a procedure has transpired. Keep in your mind that you might be in able to own a job more quickly by maneuvering to only a small organization. You also desire a stand out restart, if you require a fantastic job for a pharmacy technician. If lab technician projects in your area are infrequent or hard to comprehend, you might like to show in an application to get an apprenticeship or internship for a way to acquire your foot in your doorway.
There's A work something which goes to just about every afternoon to make dollars, but often doesn't have some potential of progress. You ought to make a resume which could make employers to believe in you personally personally and also to supply you the occasion to land your first real occupation. Some gains may be supplied by employers, but also the practice is not habitual, as stated by the BLS.
Internships in pharmaceutical search associations supply you having an opportunity to acquire handson knowledge in the field. A internship can be a prospect for becoming competed in a region of your variety. Summertime internships for senior school students supply an effortless method to develop also the abilities that's essential for earning cash and knowledge.
You have to conduct a research regarding the endeavor that is available and also the company. Answer and produce the employer aware of any expert education that is on-going that you may be competent . In spite of the amount of civil service places a single program must be finished by you. Others want membership or a commission, although some CE apps have no price. Choosing the culinary regime that is very appropriate is your very first step to establishing a livelihood. For non-medical laboratory technician rankings, you can need to accomplish a training program that is straightforward.
Use the latest edition of a text book. Allowed it's going to be tricky to devote the information of expertise specially in case you're students applying to a exact first job you are ready to rely on any voluntary or even part tasks you might have held before. Make certain you work with quite a few of resources including advice about both inpatient and health care providers. Online resources are available that will help you. Even the CCS review resources incorporate both publications and internet e-courses.
Since that you never even have a wonderful deal of encounter you really ought to present your appetite. For the hospital housekeeper's post, it's essential that you include all of the ability you have in associated fields. Granted if you applying to a first endeavor however, it'll be tricky to put in statistics of experience, it will be potential to count in professions or some other area time you'll have held at the past. Tailoring your objective when emphasizing experience, the skills and qualities you've got, can improve the chances of an employer noticing your resume. It features a drugstore technician externship in a medical facility setting. Staffing experiences usually require you to offer references, educational credentials and info .
You won't ever understand who may have an idea. You might manage to raise your likelihood of becoming hired by a internship. Becoming dealing using technology is very valuable to your career results.
LPN training is going to be described as a blend of blog experience and classroom learning and can be finished in one year. In some nations, it really is compulsory to own a whole certification. For some medical lab technician jobs, just before you are able to work you should get a certification exam . The pharm tech exam isn't simple, and it's going involve prep and diligent studying. Talk with those individuals that have taken this test.
Make a decision as to what kind of lab technician you wish to grow to be. You will find different sorts of laboratory specialists. In distributing an application for a very original job if you're an entry-level medication technician, then you may be helped by the following resume!
Laboratory techs need lots of specialized knowledge to perform their jobs safely and correctly. They must know how to fix, run, troubleshoot and maintain the gear. Be cautious to information Cosmetic techs want to present superb customer support and understand just how to stay arranged. Vet techs are trained deliver info in regards to your own pets' wellness and also to talk with patients. Most vet techs utilize digital, in place of analog, thermometers, to allow for a fast fever scanning.


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There has been a lot attention given to the value of a traditional four-year college education. For the past thirty years or so, it has been thought of having three primary purposes: preparing students for a career, providing a social experience, and imparting civic-mindedness. However, with the rising cost of tuition and associated student indebtedness, the… Continue Reading
Latest in: Education
The de facto college degree is getting squeezed from two directions: employers requiring professionals to build new-age technical skills (where there is currently a skills gap); and typical students who are faced with the rising cost of a traditional college degree and must take on significant debt. Moreover, according to a recent Gallup poll, a… Continue Reading
I think there are two ways to look at Common Core Standards Initiative. First, you have transparent and accessible standards available to the general public on a website. Adopting Common Core is compelling for this reason alone. It is powerful because parents, teachers, administrators and even students understand students' learning expectations by grade and subject.… Continue Reading
Common Core State Standards nail down precisely what primary and secondary education (K – 12) students are expected to learn in mathematics, reading, and writing. Learning related transferable skills is the primary objective of a K – 12 education because it is a foundation for higher education or training and eventually a career. On their… Continue Reading
Utilizing personalized learning is more realistic with online learning platforms. Personalized learning can be broken down in two ways. First, there is learning on a course level where a teacher or system responds to the learning aptitude of its students as they teach them. For example, a teacher assigns each student a problem set based… Continue Reading
Recently I wrote a blog, A Standardized Test To Identify Reasons For The Skills Gap, and in this blog I want to dig a little deeper regarding the CLA+: a standardized test used to assess transferable skills learned in college. Colleges incorporate teaching these skills in their "general education requirement", a collection of courses usually taken… Continue Reading
One compelling reason to move towards online learning is it introduces three feedback loops: learning platform to student, learning platform to teacher, and learning platform to course designer. [1] Utilizing feedback from online learning platforms has the potential to revolutionize the learning experience in all stages of education. In primary and secondary education, it helps… Continue Reading


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Marshall Ferret Bell Collar, Red
Marshall Ferret Bell Collar,
Marshall bell collars are designed specifically for the anatomy of ferrets and allows them to be heard when outside of their cage. Made with 3/8-inch sturdy flat nylon and fully adjustable with quick snap buttons, these collars are guaranteed to fit any size ferret.
- Color: Red
- Brand: Marshall Pet Products
Sandia Pet Products 3/8" REGULAR Red Ferret Collar with Bell - Adjustable 6 to 8 Inches
Blueberry Pet 2019 New 6 Colors The Most Coveted Designer Mixed Metallic Thread Dog Collar in Sparkling True Red with Metal Buckle, Neck 13-16.5", Adjustable Collars for Dogs
Blueberry Pet 2019 New 6 Colors The Most
Want your pooch to look fancy, yet elegant? Dress him/her up with this designer dog collar! The Most Coveted Collection is a timeless classic with a vintage yet chic twist. Style wise, it's a must-have for pet parents who love luxurious-vibe that contemporary look brings. Made with tinsel thread webbing, this special and beautiful dog collar will soon become your pooch's wardrobe staple in no time! It also features great structure such as the belt-like webbing - as if you are putting on a belt on your loved pups! The vintage golden hardware of buckle and BP tag is selected to offer the premium quality. The collar is suitable for most kinds of breed. Small breeds like poodles, yorkshire terriers, corgis, shih Tzu, spaniels, pugs, chihuahuas, maltese, russell terriers etc.; medium breeds like french bulldogs, bulldogs, beagles, dachshunds, collies, bull terriers, Australian shepherds, boxers etc.; large breeds like labrador, labrador retrievers, german shepherd, golden retrievers, rottweilers, huskies, great danes etc.
- Color: Pack of 1 - Sparkling True Red
- Brand: Blueberry Pet
Sandia Pet Products 3/8" PETITE (For A Small Female Or Young Ferret) Red Ferret Collar with Bell - Adjustable
Marshall Ferret Harness and Lead, Red
Marshall Ferret Harness and Lead,
Marshall harness and lead sets are designed specifically for the anatomy of ferrets. Made with 3/8-inch sturdy flat nylon and fully adjustable with quick snap buttons, these collars are guaranteed to fit any size ferret.
- Color: Red
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Margelo Pet Classic Solid Color Ferret Collar with Bell - Adjustable
Margelo Pet Classic Solid Color Ferret Collar with
Package Included: 1 Red Collar +1 Black CollarInformationDesigned to prevent injuries and ensure safety of pet when outdoor. Buy Margelo cute Collar with Safely Bell to enjoy walking with your lovely pet! Please never leave your ferret unattended outside. Notice* The actual color may vary slightly from the online image due to monitor color settings. * Totally hand-made. Please allow slight measurement deviation for the data. If you want to know more details or have any questions, please look through the detailed page or contact the customer service.
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Sandia Pet Products 3/8" PETITE (For A Small Female Or Young Ferret) Raspberry Ferret Collar with Bell - Adjustable
RYPET Small Animal Harness and Leash - Soft Mesh Small Pet Harness with Safe Bell, No Pull Comfort Padded Vest for Small Pet Red M
RYPET Small Animal Harness and Leash - Soft
RYPET harness is made of mesh material which is breathable to keep your small pet cool on hot days, but also provides insulation for those chilly occasions. And it's going to be one comfortable walk for your pet ! Unlike collars that have the tendency to strain your pet's neck, this safety harness give more space to adjust the buckle to adapt your pet's body and evenly distributes the pressure around the chest to prevent any kind of choking. Package Including: 1 x small animals harness; 1 x small animals leash; 1 x safe bell; Quality Guarantee: We accept the replacement for any breakage or fragmentary of the item with original package and related accessories within one week, but not include any damages to human factors. If you are looking for the best quality small animal harness and leash, here it is! Click Add To Cart at the top of the page to get yours!
- Color: Red
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StrawberryEC 2PC Extra Small Dog and Cat Collar with Cute Plaid Bowtie. Adjustable 5 Holes to Also Fit Puppy and Kitten. Quality PU Leather and Durable Polyester (X-Small, 2PC-Red Plaid)
StrawberryEC 2PC Extra Small Dog and Cat Collar
Distributed Exclusively By StrawberryEC, INC VERY CUTE X-SMALL CAT & DOG COLLAR WITH DARLING BOWTIE design. Your little guy or gal will be so proud wearing this designer, fashionable collar. He or she will get lots of compliments and pets. You can bet it will be their favorite collar they will want to wear again and again. This collar is great for special occasions like trips to town, visits with grandmother, and birthdays. It's also durable and appropriate for everyday wear. HIGH QUALITY POLYESTER BLEND IN PAID STYLE combined with PU Leather and zinc alloys style buckle and grommets. The shiny buckle adds greatly to the overall fancy look of this collar. The length is adjustable 8.8-10.8 inches with 3/8 inch width. EXTRA SMALL FOR YOUR LITTLE GUY OR GAL. It's often hard finding attractive, cute collars for very small dogs and cats. This one is actually small enough to adjust down to kittens and puppies. Just right for most smaller breeds of dogs and almost all cats. You get 5 holes of adjustment that make this easy to get a comfortable custom fit. Just right for rabbits, ferrets, and other small pets. 100% MONEY BACK GUARANTEE ensures this will be your best bet for a no-risk purchase. Many customers love this collar so much they return to buy a second and third collar. HURRY...
- Color: 2PC-Red Plaid
- Brand: StrawberryEC
Stock Show Small Pet Outdoor Walking Harness Vest and Leash Set with Cute Bowknot Decor Chest Strap Harness for Rat Ferret Squirrel Hamster Clothes Accessory, Red Plaid
Stock Show Small Pet Outdoor Walking Harness Vest
This set contains a harness with a cute vest harness and a matching colored leash. It is the most stylish way to walk your favorite pet family member. The harness and the leash are designed to walk your critters safely. The adjustable size fits smaller ferrets, skunks, guinea pigs, rabbits, and other critters. The H shaped Harnesses are a comfortable alternative to collars for smaller sized pets because they distribute the weight of the pet evenly throughout. Our harnesses are designed to be gentle and put less stress on the pet when it pulls on the leash. Made of soft, lightweight and breathable material. Machine washable for easy cleaning. We highly recommend measuring your pet neck and chest size precisely with a flexible measuring tape to ensure the best fit. Contact us if you have any questions. We are happy to help!
- Color: Red Plaid
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Ferret Wrestling
Garret & Tinkerbell are in a wrestling match. Garret is in the green collar and Tinkerbell in the...
Ferret Training 101: Ferret Spin - Ferret Trick
Visit the Ferret-World website on http://www.Ferret-World.com Also join the Ferret-World communit...
Ferreting for Rabbits
Today on Realtree Global Hunting, we are ferreting for rabbits Ferreting has been a popular pest...
Our new babies, Zoe and Taz. (Taz has the red collar and Zoe has the black collar).
Smart begging ferret
This super smart female ferret knows that most of the time when a human being she owns is about t...


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AddrycNew Members
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About Addryc
Addryc started following AoS 2 - Stormcast Eternals Discussion
AoS 2 - Stormcast Eternals Discussion
Addryc replied to Chris Tomlin's topic in OrderHi All! First-time poster but I have been following this thread for a while - y'all provide so much insight and knowledge sharing - THANK YOU! So I have what I hope is an interesting list challenge for y'all - there is a themed event for Forbidden Power at the Warhammer Citadel Weekender, titled 'Magic without end' - the general details are below: I want to do something narratively appropriate so I'm looking at building a Sacrosanct-only list, built around Stormsire's Cursebreakers. I am thinking (rough composition only): But I have NO Idea if this will be effective, or what spells could be a good synergy. I have not played a LOT of AoS but I have been collecting for a while and this is a good tournament goal for a painted army. Any suggestions or ideas for making this list cooler is very much appreciated!


text: Tim Burgan wrote: >Hello, > >Now this problem I'm having is weird. > >In Opera 7.2 and Mozilla 1.4 (Win) my site [1] appears to be displaying >fine. >In Internet Explorer 6 (Win) it doesn't render - it displays the source >code. > >Why on earth would this be happening?? > >[1] <http://www.greaterthani.com/02_relaunch/index.htm> > whip out that xml declaration (why is it there if this is an HTML page - it is optional and I think not recommended even for xhtml?) and it works fine > >Thanks >Tim Burgan > > > -- ------------------------------ Paul Bennett Internet Developer Teltest Electronic Design ------------------------------ Email: paul at teltest.com Phone: 64 4 237 4557 Web: http://www.teltest.com Wap: http://wap.teltest.com


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Tag Archives: Adventure
America fights pirates!
Have you guys seen this? Make with the clickity click and check that crap out. Welcome back. Have some hot chocolate. Not what I meant, but this is what showed up on Google In case you didn't clicky click and … Continue reading
Posted in Uncategorized Tagged Adventure, In Today\'s News Leave a comment
I'mma read Twilight
Okay, here's a thing: I'm gonna get the Twilight books, and I'm going to read the crap out of them. Sooo… they're about AppleFlowerRibbonChess? Some of you might be wondering, "Why, Tony?! We love you! You don't have to do … Continue reading
Posted in Uncategorized Tagged Adventure, Disappointment 2 Comments
Good gravy, it's gorgeous outside
I cover everything but sports here at the Advocate, which means the sports guys are the ones who spend most of the time being paid to go outside. Me? I do most of my work on the internet or over … Continue reading
Posted in Uncategorized Tagged Adventure, Shenanigans: Cheeky and Fun Leave a comment
This guy was the bomb
His name was Walter Wellman. He was born in Ohio in 1858, and he was an adventurer. I LOVE THAT TITLE. I want that title. According to dictionary.com, an adventurer is one who "has, enjoys, or seeks adventure." How do … Continue reading
Posted in Uncategorized Tagged Adventure, Heroes of History Leave a comment


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