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Thanks to the intervention of a federal judge, the Trump administration has been prevented for now from transferring to Saudi Arabia a U.S. citizen seized in Syria and suspected of fighting for Islamic State. Instead of seeking to overturn Thursday's ruling by U.S. District Judge Tanya Chutkan, the administration should either charge the man with a crime or give him his freedom.
The man, who is known in court documents as John Doe, also holds Saudi citizenship. He surrendered to a Syrian militia backed by the United States in September and was turned over to the U.S. military. It declared him an "enemy combatant" and placed him in a military prison in Iraq, but apparently it lacks the evidence necessary to charge him with a crime. According to his lawyers, Doe claims he was kidnapped by Islamic State and denies fighting alongside the militants.
Apparently the U.S. government sees moving Doe to another country as a convenient way to resolve the dilemma created by the difficulties of charging him with a crime or, alternatively, imprisoning him as an enemy combatant, which also would eventually require the government to defend its position in court. That's because, in a 2004 decision called Hamdi vs. Rumsfeld, the Supreme Court ruled that a U.S. citizen held as an enemy combatant must have a right to challenge his confinement.
But exiling a U.S. citizen without due process is just as much a violation of due process as imprisoning him without trial. The "solution" of handing Doe over to Saudi Arabia against his will is no solution at all. As Chutkan remarked during a hearing Thursday before issuing her ruling: "It's not release if you're simply giving him over to another jailer."
In opposing the transfer, the American Civil Liberties Union, which is representing Doe, made two principal arguments: that his detention and possible transfer are illegal because the government lacks the legal authority to wage war on Islamic State, and that regardless of the legality of the war, Doe's forced transfer to another country in the absence of any criminal charges against him there is a violation of his constitutional rights. (His case differs from others in which the courts have upheld turning over a U.S. citizen to a foreign country where charges were pending or there was an extradition agreement.)
"The government has no legal authority to detain this U.S. citizen in the first place, and it clearly lacks any legal authority to transfer him to the custody of another government," said Jonathan Hafetz, an attorney for the ACLU.
The first assertion is more debatable than the second. Congress has been remiss in failing to explicitly authorize the war against Islamic State undertaken by former President Obama and continued by President Trump.
But even if one accepts that the war against Islamic State is on sound legal footing, the proposed transfer of Doe to Saudi Arabia is objectionable. An American citizen suspected of taking up arms on the wrong side is still entitled to due process (and he doesn't forfeit any of his constitutional rights because he has citizenship in a second country).
And there are ways to prosecute Americans who aid the enemy. In the Hamdi case, the late Justice Antonin Scalia pointed out that, in addition to treason, federal law criminalizes "various acts of war making and adherence to the enemy." U.S. citizens also can be charged with other criminal offenses, including providing material support for terrorism.
If the government can establish that the man known as John Doe violated any of these laws, it should file charges against him in federal court, where it successfully has prosecuted several previous terrorism cases. If, however, it is unable to make a case, it should release him — and give up the idea of handing him off to "another jailer" in another country.
Los Angeles Times


text:
Surveyors in Union County, NJ
Comprehensive list of Union County, New Jersey based commercial and residential companies offering quantity, land, property, building & construction surveying services.
Union County, NJ
- Berkeley Heights
- Clark
- Cranford
- Elizabeth
- Fanwood
- Garwood
- Hillside
- Kenilworth
- Linden
- Mountainside
- New Providence
- Plainfield
- Rahway
- Roselle
- Roselle Park
- Scotch Plains
- Springfield
- Summit
- Union
- Vauxhall
- Westfield
Business Listings
William Held Associates. is a Licensed Professional New Jersey Land Surveying and Professional Planning company with an office situated in Central New Jersey. We provide land surveying and planning services throughout most of New Jersey. We are family owned & operated for over forty years. We look ahead to conducting business with you.
Established in 1991 by James R. Watson, P.L.S., P.P., who currently serves as President and Chief Executive Officer, EKA Associates has gained a reputation of delivering excellence in engineering and surveying for both the private and public sectors. Whether designing a capital improvement project or a major site plan for a commercial or domestic development, EKA ensures that each project is completed
Full service engineering and land surveying firm centrally located in Monmouth County. Our Professional Land Surveyors blend time-proven practices and skills with the use of electronic total data stations, fully robotic instruments, and global positioning equipment to deliver high levels of precision, increased production, and timely service.
Braginsky Surveying is a New Jersey based firm which provides a huge array of Land Surveying Services. Weather your searching for a survey for a real estate closing, want to subdivide your land, or looking to evolve huge domestic or commercial properties, Braginsky Surveying provides all these services and many more.
Kupper Associates has been actively serving private and public owners as Engineers, Planners, Surveyors and Construction Managers since 1924. It is one of the bigger independently- owned consulting engineering companies in New Jersey. Kupper provides a complete service approach to project implementation, from planning, feasibility and public acceptance through design, permitting, construction management
Pinpoint Surveying is an expert land surveying company delivering solutions to each of Northern and Central New Jersey. Our goal is to supply superior service to our customers using the latest field and office equipment, keeping us on the frontier of innovative technology. Our team has over twenty years of knowledge in delivering professional and reliable surveying services.
Established in 1975 our Professional Engineering and Land Surveying service offers title land surveying services for real estate closings, preparation of site plans and topographical surveys throughout northern New Jersey including the counties of Essex, Bergen, Passaic, Morris, Sussex, Monmouth and Ocean County.
The company of Keller and Kirkpatrick, set up in 1972, is one of the major engineering, surveying, planning and landscape architecture companies in New Jersey. Keller & Kirkpatrick is unique in providing guaranteed on-time project completion. The capability to provide the highest quality work on a fixed schedule can only be achieved through knowledge and a commitment to professionalism in every kind
Caulfield Associates, LLP is a family run and operated professional land surveying company that has been serving Northern and Central New Jersey since 1933. The company supplies surveying and mapping services for a range of commercial and domestic purposes, utilizing the latest technology and field procedures including Global Positioning, GPS and ariel photogrametry.
Chester, Ploussas, Lisowsky Partnership, LLP CPL is currently undergoing extensive upgrades to our website. For over thirty five years, the common thread that has unified the progress of this company has been its professional expertise in all aspects of its services and its capability to supply these services in varying markets.
We're making an effort to present you with our whole spectrum of offers as soon as possible. At this point we can supply you with information regarding Our Engineering and Land Surveying Services. We are looking forward to hearing from you. If you are not familiar with our firm and your 1st contact with us is online: We would be glad to hear from you!
With a capable team of specialists, Stires Associates, P.A. has the expertise to take your next project from Field to Finish. Even before property acquisition, Stires Associates, P.A. will work with our customers to ensure their project goals. Our team is continually updating their technical knowledge as well as their expertise in Local, State and Federal regulations and criteria.
The Bleeker Architectural Group. is a multi-disciplined limited liability corporation that specializes in delivering innovative architectural, planning, and surveying solutions to our public and private customers. The firm was established in 1999 by its Principal, John G. Bleeker, R.A., P.P., L.S. Formerly known as Bleeker-Evans Partnership, it has been delivering design services to Passaic and Bergen
Please feel free to look around. Whether you have a question, need information or are in need of surveying services, we hope you find the site helpful and informative. JT Surveying is a tiny, owner operated surveying and mapping firm situated in Central New Jersey. Our mission is to supply a standard of accuracy, excellence and integrity while providing needed services in a timely fashion.
Koestner Associates is one of New Jersey's oldest engineering and land surveying companies. We have a long and noteworthy history, a history we believe exemplifies our pride in and dedication to the industry, our business and the people of New Jersey. Our Mission To supply customers with civil engineering and land surveying services in a timely manner, accurate in detail, in accordance with applicable
Gluckler & Den Bleyker has been providing high-quality surveying services for over 50 years. Our customer can rely on us for a timely turnaround period and a survey showing detailed information of the property, enabling a proper analysis helping to expedite your real estate closing. We service residential or commercial customers all over New Jersey. Our extensive records, cover Bergen, Essex, Hudson
This web page is optimized for a monitor resolution setting of 1024 x 768 or better.
3 Wire Surveying. is a complete service professional land surveying company servicing central and northern parts of New Jersey. Engineers and Planners With ten years of land surveying experience working in engineering and planning companies and our wealth of cad design experience, we can supply a product that will exceed the expectations of project engineers and planners.
Lakeland Surveying. supplies domestic land surveying services throughout New Jersey. Utilizing leading edge technology, including state of the art robotic total stations, GPS units and software solutions, Lakeland Surveying. supplies our customers with the highest standards of professional service in a timely fashion.
- Lakeland Surveying provides prompt and professional service at a very reasonable price.
Set up in 1987, Mountain View Layout supplies comprehensive construction layout and land surveying services throughout New Jersey as well as southern New York and eastern Pennsylvania. Utilizing leading edge technology, including state of the art robotic total stations, GPS units and software solutions, Mountain View Layout supplies our customers with the highest standards of professional service.


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VCA Charles Towne Animal Hospital
850 Savannah Hwy | Charleston, SC 29407Call 843-608-0934
|8:00am - 6:00pm
|8:00am - 6:00pm
|8:00am - 6:00pm
|8:00am - 6:00pm
|8:00am - 6:00pm
|8:00am - 3:00pm
- Animal Boarding
- Animal Flea Control
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- Spaying/Neutering
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At VCA Animal Hospital, we look forward to welcoming you, your dog, cat and other pets. Everyone on our staff believes that the better we get to know your pets, the better we can provide the best possible health care for them. In fact, when you come in, you'll see exactly why our veterinarians are praised for their kind and thorough care. We'll give you and your pet all the time you need, and never rush through an exam. We'll also carefully track the course of your pet's care, which is so important to your pet's long-term health.
In every case, you'll be served by experienced, knowledgeable members of our staff. Our veterinarians, veterinary technicians and other pet-friendly personnel are trained to the highest standards. Their knowledge of the latest veterinary medicines and procedures ensures that all our pet patients get the best in prevention and healing methods.
Medical Director
DVM, Auburn University
Dr. Dennis Feinberg was born and raised in Alabama. He received his undergraduate degree from Emory University. He then attained a degree in pharmacy from Auburn University. His veterinary degree was from Auburn University in 1975. His areas of interest are surgery, internal medicine and preventative care. Dr. Feinberg is married and has two daughters. Additionally two English Springer Spaniels Rooney and Lollie are family members. He enjoys exercise, reading and volunteerism in and out of the field of veterinary medicine.
Associate Veterinarian
DVM, University of Georgia
Dr. Kim Fordney is a South Carolina native from Hilton Head. After graduating from the University of Georgia, College of Veterinary Medicine in 2002, she joined our team at Charles Towne Veterinary Clinic. She and her husband Sam share their home with their sons, Bennett & Simon, and daughters Sydney & Taylor. They have one dog named Kramer and a cat, Highland. In her free time she enjoys horseback riding, fishing and reading.
Associate Veterinarian
DVM, Michigan State University
Dr. Terry Kobza grew up in western Michigan. She attended the University of Michigan for her undergraduate degree and Michigan State University for her Doctor of Veterinary Medicine. She has been practicing in the Charleston area since her graduation in 1996 and joined the team at Charles Towne Veterinary Clinic in 2004. She lives in Mt. Pleasant with her husband, John, and her six cats. She enjoys bicycling, swimming, and travel.


text:
|World Trail Orienteering Championships
|sporting event
|Organised by
This article may be expanded with text translated from the corresponding article in Czech. (December 2017)Click [show] for important translation instructions.
The World Trail Orienteering Championships (WTOC) were first held in 2004 and annually since them. The majority of the championships were held in Europe, with 2005 the only exception up to date.
The current championship events are:
|Location [1]
|2004 (in conjunction with FootO WOC 2004)
|September 15–18
|2005 (in conjunction with FootO WOC 2005)
|August 9–11
|2006 (in conjunction with WMTBOC 2006)
|July 9–14
|2007 (in conjunction with FootO WOC 2007)
|August 17–26
|2008 (in conjunction with FootO WOC 2008)
|July 12–26
|2009 (in conjunction with FootO WOC 2009)
|August 18–23
|2010 (in conjunction with FootO WOC 2010)
|August 4–6
|2011 (in conjunction with FootO WOC 2011)
|August 13–20
|June 6–9
|2013 (in conjunction with FootO WOC 2013)
|July 6–14
|2014 (in conjunction with FootO WOC 2014)
|July 5–13
|June 22–28
|2016 (in conjunction with FootO WOC 2016)
|August 20–28
|July 10–15
|2018 (in conjunction with FootO WOC 2018)
|August 6–10
|June 23–29
This was called the "individual competition" before 2010.
|Number of controls (day 1)
|Number of controls (day 2)
|20 controls, 2 TCs [17]
|20 controls, 2 TCs [18]
|20 controls, 2 TCs [19]
|20 controls, 2 TCs [20]
|2012 [21]
|20 controls (1 voided), 2 TCs [22]
|23 controls, 2 TCs [23]
|2013 [24]
|20 controls, 2 timed stations, 4 TCs [25]
|19 controls, 1 timed station, 2 TCs [26]
|2014 [27]
|12 controls (1 voided), 1 timed station, 2 TCs [28]
|20 controls, 2 timed station, 3 TCs [29]
|2015 [30]
|26 controls, 2 timed stations, 6 TCs [31]
|26 controls, 2 timed stations, 6 TCs [32]
|2016 [33]
|21 controls (1 voided), 1 timed station, 3 TCs [34]
|28 controls, 1 timed station, 3 TCs [35]
|2017 [36]
|21 controls (2 voided), 1 timed station, 3 TCs [37]
|28 controls, 3 TCs [38]
|Number of controls (day 1)
|Number of controls (day 2)
|20 controls, 2 TCs [39]
|20 controls, 2 TCs [40]
|20 controls, 2 TCs [41]
|20 controls, 2 TCs [42]
|2012 [43]
|20 controls (1 voided), 2 TCs [44]
|23 controls, 2 TCs [45]
|2013 [46]
|20 controls, 2 timed stations, 4 TCs [47]
|19 controls, 1 timed station, 2 TCs [48]
|2014 [49]
|12 controls (1 voided), 1 timed station, 2 TCs [50]
|20 controls, 2 timed station, 3 TCs [51]
|2015 [52]
|26 controls, 2 timed stations, 6 TCs [53]
|26 controls, 2 timed stations, 6 TCs [54]
|2016 [55]
|21 controls (1 voided), 1 timed station, 3 TCs [56]
|28 controls, 1 timed station, 3 TCs [57]
|2017 [58]
|21 controls (2 voided), 1 timed station, 3 TCs [59]
|28 controls, 3 TCs [60]
All competitors, regardless of disability, participate in one single class only since there is no physical movement involved in the competition process.
|Number of controls (qualification)
|Number of controls (final)
| 2010
(World TempO Trophy)
| 2011
(World TempO Trophy)
| 2012 [61]
(World TempO Trophy)
|8 timed stations, 24 TCs
|2013 [62]
|8 timed stations, 24 TCs
|5 timed stations, 15 TCs
|2014 [63]
|6 timed stations, 24 TCs [64]
|5 timed stations, 25 TCs [65]
|2015 [66]
|7 timed stations, 28 TCs [67]
|7 timed stations, 35 TCs [68]
|2016 [69]
|4 timed stations, 20 TCs [70]
|5 timed stations, 30 TCs [71]
|2017 [72]
|5 timed stations, 25 TCs [73]
|6 timed stations, 30 TCs [74]
Orienteering is a group of sports that require navigational skills using a map and compass to navigate from point to point in diverse and usually unfamiliar terrain whilst moving at speed. Participants are given a topographical map, usually a specially prepared orienteering map, which they use to find control points. Originally a training exercise in land navigation for military officers, orienteering has developed many variations. Among these, the oldest and the most popular is foot orienteering. For the purposes of this article, foot orienteering serves as a point of departure for discussion of all other variations, but almost any sport that involves racing against a clock and requires navigation with a map is a type of orienteering.
Sport in New Zealand largely reflects its British colonial heritage, with some of the most popular sports being rugby union, rugby league, cricket, football (soccer), basketball and netball which are primarily played in Commonwealth countries. New Zealand is a small nation but has enjoyed success in many sports, notably rugby union, rugby league, cricket, America's Cup sailing, world championship and Olympics events and motorsport and softball.
The World Orienteering Championships is an annual orienteering event organized by the International Orienteering Federation. The first World Championships was held in Fiskars, Finland in 1966. They were held biennially up to 2003. Since 2003, competitions have been held annually. Participating nations have to be members of the International Orienteering Federation (IOF).
The Junior World Orienteering Championships are an orienteering competition held every year. They were first held in 1990. Entry is open to national teams aged 20 and below as of 31 December in the year of competition. Representative countries must be members of the International Orienteering Federation (IOF).
The United Kingdom has been awarded, or is bidding to hold, a number of major international sporting events during the 2010s leading to an idea of a 'Golden Decade' in British sport. The idea of the golden decade has been discussed in many newspapers and has been mentioned by former Prime Minister Gordon Brown and Lord Coe.
The European Orienteering Championships were first held in 1962. They have been held biennially since 2000. From 2020, the European Orienteering Championships will be held annually, with sprint events and forest events in alternate years
Ski orienteering (SkiO) is a cross-country skiing endurance winter racing sport and one of the four orienteering disciplines recognized by the IOF. A successful ski orienteer combines high physical endurance, strength and excellent technical skiing skills with the ability to navigate and make the best route choices while skiing at a high speed.
The World Ski Orienteering Championships (Ski-WOC) is the official event to award the titles of World Champions in ski orienteering. The World Championships is organized every odd year. The programme includes Sprint, Middle and Long Distance competitions, and a Relay for both men and women. The first Ski-WOC was held in 1975.
Olav Lundanes is a Norwegian orienteering competitor, hailing from Ålesund. He has won gold medal at the World Orienteering Championships eight times and the European Orienteering Championships four times.
Magne Dæhli is a Norwegian orienteering competitor, ski-orienteer and cross-country skier. His achievements include five medals in the relay at the World Orienteering Championships, of which three are gold medals. His best individual performances include a silver medal in the long distance from the European Orienteering Championships, and a fourth place in both the middle and long distances from the world championships.
Trail orienteering (TrailO) is an orienteering sport that involves precise reading of an orienteering map and the corresponding terrain. Trail orienteers must identify, in the terrain and in the presence of decoys, control points shown on the map. TrailO involves navigation skills but unlike most other forms of orienteering, it involves no point to point racing and little or no route choice. It is conducted usually on trails and because the objective is accuracy, not the speed of physical movement, the sport is accessible to physically disabled competitors on equal terms as able-bodied.
The Junior World Ski Orienteering Championships were first held in 1994. Since 1998, competitions have been held annually. Representative countries must be members of the International Orienteering Federation (IOF).
Maja Møller Alm is a Danish orienteering competitor who has won seven World Championships and two World Games titles. She is especially known for her four gold medals on the sprint distance, where she has won the title four years in a row: 2015, 2016, 2017 and 2018. She is also a medalist from the Junior World Championships. Since 2006 she has been coached by Danish national coach Lars Lindstrøm. Alm is remarkable for her dominant running speed, which gives her a great advantage over other competitors at the sprint distance.
Mountain bike orienteering is an orienteering endurance racing sport on a mountain bike where navigation is done along trails and tracks. Compared with foot orienteering, competitors usually are not permitted to leave the trail and track network. Navigation tactics are similar to ski-orienteering, where the major focus is route choice while navigating. The main difference compared to ski-orienteering is that navigation is done at a higher pace, because the bike can reach higher speeds. As the biker reaches higher speeds, map reading becomes more challenging.
Foot orienteering is the oldest formal orienteering sport, and the one with the most "starts" per year. Usually, a FootO is a timed race in which participants start at staggered intervals, are individually timed, and are expected to perform all navigation on their own. The control points are shown on the orienteering map and must be visited in the specified order. Standings are determined first by successful completion of the course, then by shortest time on course.
Mika Tervala is a Finnish mountain bike orienteering competitor, World Champion and European Champion. He won individual gold medals at the 2002, 2006 and 2007 World Championships, and a gold medal in the relay in 2004, 2005 and 2006. Mika coaches the Finnish national mountain bike orienteering teams since spring 2008.
The World Mountain Bike Orienteering Championships is the official event for awarding World Champion titles in mountain bike orienteering. The World Championships, also known as WMTBOC, were first held in 2002, and since 2004 they have been organized annually. The programme includes Long distance, Middle distance, Sprint, and a Relay for both men and women.
Guntars Mankus is a Latvian orienteer, rogainer and adventure racer.
World Masters Orienteering Championships are an annual official world championship event of the International Orienteering Federation for orienteering runners aged 35 and over.


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1801 $10 BD-2, R.2, MS62 PCGS....Click the image to load the highest resolution version.
1801 Capped Bust Right Eagle, MS62
1801 $10 BD-2, R.2, MS62 PCGS. Bass-Dannreuther Die State
a/a, scarce early die state without vertical spines in the cap.
Mint records indicate a substantial mintage of 44,344 Capped Bust
Right eagles were produced in 1801, but some of those coins may
have been struck from dies with earlier dates. The dies were
becoming more durable than they were in prior years, and only two
die varieties are known for the date. This coin represents the more
available BD-2 variety, with star 1 away from the bust and star 13
close. The popular BD-2 variety has a surviving population of
600-800 examples in all grades, making it a logical choice for type
collectors. This was the only use of the obverse die, but the
reverse was used again to strike the BD-3 variety of 1803.
Popular BD-2 Type Coin
The present coin is an impressive MS62 example, with sharply detailed design elements in most areas and just the slightest softness on some of the star centers. The antique-gold surfaces are lightly marked for the grade and retain much of their original mint luster. Population: 73 in 62 (2 in 62+), 74 finer (7/18).
From The Washington Spring Collection. (NGC ID# 2627, Variety PCGS# 45734, Base PCGS# 8564)
Weight: 17.50 grams
Metal: 91.67% Gold, 8.33% Copper
Learn more at the Newman Numismatic Portal at Washington University in St. Louis.
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Superb cuts, attractive prices and feminine fits form the basis of an ELLABEE garment. Styling for the line is eclectic with the most in-vogue features of each season being incorporated into every piece to provide the most up-to-date look.
ELLABEE's unique fashion celebrates the woman who believes in herself, knows who she is and fully understands what she wants.


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There is no notification being sent to me when an order is received through SPS Commerce. Therefore, I have orders that have been sitting for almost a month.


text: On Sat, Jan 28, 2017 at 9:57 AM, Dmitry Golovin <dima at golovin.in> wrote: > At this point I'm able to link Linux kernel with LLD and objcopy doen't > give me any errors. > > The versions are: > > Linux 4.10.0-rc5 (+ applied the patch from my previous message) > LLD 5.0.0 (https://github.com/llvm-mirror/lld > db83a5cc3968b3aac1dbe3270190bd3282862e74) (+ applied D28612) > GNU objcopy (GNU Binutils) 2.27 > > The problem is that the resulting kernel doesn't boot. Does anybody have > any suggestions on how to debug it or any guesses what did go wrong while > linking? > As far as different things that can go wrong, some things to consider: - LLD's output binary has the same (or similar) data as the BFD/gold output binary. E.g. if the LLD binary is only half as big (or the PT_LOAD's that the booloader looks at are half as bit), LLD might not be putting things into the output or into the right output sections. - The bootloader is looking for something in the dynamic symbol table, but it isn't there. LLD might be resolving symbols differently. - Section contents are different between LLD and BFD/gold. E.g. for freebsd there is a "linker set" section which contains pointers to a bunch of metadata structs that are needed. LLD was not relocating these correctly because the symbols were not ending up in the output or something like that (I forget exactly; Michael might remember better). -- Sean Silva > > Regards, > Dmitry > > > 28.01.2017, 05:48, "Sean Silva via llvm-dev" <llvm-dev at lists.llvm.org>: > > > > On Fri, Jan 27, 2017 at 1:31 PM, Rui Ueyama <ruiu at google.com> wrote: > > Sean, > > So as you noticed that linker script tokenization rule is not very trivial > -- it is context sensitive. The current lexer is extremely simple and > almost always works well. Improving "almost always" to "perfect" is not > high priority because we have many more high priority things, but I'm fine > if someone improves it. If you are interested, please take it. Or maybe > I'll take a look at it. It shouldn't be hard. It's probably just a half day > work. > > > Yeah. To be clear, I wasn't saying that this was high priority. Since I'm > complaining so much about it maybe I should take a look this weekend :) > > > > As far as I know, the grammar is LL(1), so it needs only one push-back > buffer. Handling INCLUDE directive can be a bit tricky though. > > Maybe we should rename ScriptParserBase ScriptLexer. > > > That sounds like a good idea. > > -- Sean Silva > > > > On Fri, Jan 27, 2017 at 11:17 AM, Rafael Avila de Espindola < > rafael.espindola at gmail.com> wrote: > > > Hmm..., the crux of not being able to lex arithmetic expressions seems to > > be due to lack of context sensitivity. E.g. consider `foo*bar`. Could be > a > > multiplication, or could be a glob pattern. > > > > Looking at the code more closely, adding context sensitivity wouldn't be > > that hard. In fact, our ScriptParserBase class is actually a lexer (look > at > > the interface; it is a lexer's interface). It shouldn't be hard to change > > from an up-front tokenization to a more normal lexer approach of scanning > > the text for each call that wants the next token. Roughly speaking, just > > take the body of the for loop inside ScriptParserBase::tokenize and add a > > helper which does that on the fly and is called by consume/next/etc. > > Instead of an index into a token vector, just keep a `const char *` > pointer > > that we advance. > > > > Once that is done, we can easily add a `nextArithmeticToken` or something > > like that which just lexes with different rules. > > I like that idea. I first thought of always having '*' as a token, but > then space has to be a token, which is an incredible pain. > > I then thought of having a "setLexMode" method, but the lex mode can > always be implicit from where we are in the parser. The parser should > always know if it should call next or nextArithmetic. > > And I agree we should probably implement this. Even if it is not common, > it looks pretty silly to not be able to handle 2*5. > > Cheers, > Rafael > > > > , > > _______________________________________________ > LLVM Developers mailing list > llvm-dev at lists.llvm.org > http://lists.llvm.org/cgi-bin/mailman/listinfo/llvm-dev > > -------------- next part -------------- An HTML attachment was scrubbed... URL: <http://lists.llvm.org/pipermail/llvm-dev/attachments/20170201/1eaff8eb/attachment-0001.html>


text:
Research Papers:
M2-like tumor-associated macrophages drive vasculogenic mimicry through amplification of IL-6 expression in glioma cells
PDF | HTML | Supplementary Files | How to cite | Order a Reprint
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Lin Zhang1,3, Yangyang Xu2, Jintang Sun1,3, Weiliang Chen2, Lei Zhao1,3, Chao Ma1,3, Qingjie Wang1,3, Jia Sun1,3, Bin Huang2, Yun Zhang3, Xingang Li2, Xun Qu1,3
1Institute of Basic Medical Sciences, Qilu Hospital of Shandong University, Jinan, P.R. China
2Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, Jinan, P.R. China
3Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital, School of Medicine, Shandong University, Jinan, P.R. China
Correspondence to:
Xingang Li, email: email@example.com
Xun Qu, email: firstname.lastname@example.com
Keywords: macrophages, glioma, vasculogenic mimicry, IL-6, PKC
Received: August 02, 2016 Accepted: November 15, 2016 Published: November 26, 2016
Vasculogenic mimicry (VM) has offered a new horizon for understanding tumor angiogenesis, but the mechanisms of VM in glioma progression have not been studied explicitly until now. As a significant component of immune infiltration in tumor microenvironment, macrophages have been demonstrated to play an important role in tumor growth and angiogenesis. However, whether macrophages could play a potential key role in glioma VM is still poorly understood. Herein we reported that both VM and CD163+ cells were associated with WHO grade and reduced patient survival, and VM channel counting was correlated to the number of infiltrated CD163+ cells in glioma specimens. In vitro studies of glioma cell lines implicated that M2-like macrophages (M2) promoted glioma VM. We found that conditional medium derived from M2 amplified IL-6 expression in glioma cells. Furthermore, our data indicated that IL-6 could promote glioma VM, as blocking IL-6 with neutralizing antibodies abrogated M2-mediated VM enhancement. In addition, the potent PKC inhibitor bisindolylmaleimide I could prevent M2-induced IL-6 upregulation and further inhibited glioma VM facilitation. Taken together, our results suggested that M2-like macrophages drove glioma VM through amplifying IL-6 secretion in glioma cells via PKC pathway.
Gliomas are a heterogeneous group of neoplasms and constitute approximately 50% of primary brain tumor [1], the most common being the highly malignant glioblastoma [2]. Despite therapeutic options have improved, the median survival among patients with glioblastoma is only 14.6 months [3]. As glioblastomas are highly vascularized tumors [4], anti-angiogenic therapies targeting endothelial cells have received much attention and investigation [5]. However, the conventional anti-angiogenic therapy, which seemed promising initially, shows transitory and incomplete efficacy [6–9]. These studies indicated that there may be other blood supply forms in tumor tissues. Maniotis et al. first demonstrated tumor vasculogenic mimicry (VM), which refers to tumor cells directly lined up to form blood vessels, independent of endothelial cells [10]. Accumulated results have shown that VM exists in various malignant tumors and is linked to resistance to anti-angiogenic therapy and the poor prognosis of cancer patients [11–13]. As an alternative to conventional anti-angiogenic therapy, the identification of molecules and signaling pathways relating to VM may offer potential therapeutic targets to improve treatment [14].
In solid tumors, the course of pathology is controlled not only by the genetic makeup of the tumor cells, but also depends on the interplay with tumor microenvironment [15]. A striking feature of tumor microenvironment is the large number of immune cells, which accumulate in tumor mass and involve in the tumor progression [16]. The most abundant immune cells infiltrating in solid tumor are macrophages, representing up to 50% of all tumor mass [16]. In glioblastoma tumor-associated macrophages (TAMs) constitute up to 30% of tumor mass [17, 18]. Moreover, TAMs infiltrated in glioblastoma tissues have been reported to express the elevated M2 cell markers, CD14, CD68, CD163, and CD204 [19]. Recent work indicated that TAMs infiltration contributed to proangiogenic effects and was associated with resistance to anti-angiogenic therapy in several solid tumors [20–22], including glioblastoma [23]. These findings suggested that targeting TAM-promoting angiogenesis in tumor progression can be a universal method to overcome anti-angiogenic drug resistance.
Another important hallmark of cancer is considerable cytokines and chemokines in tumor microenvironment [24, 25]. Increasing data described that these immune mediators could create a favorable environment to support tumorigenesis by driving the functions of immune cells and tumor cells [26, 27]. One such factor is the pleiotropic cytokine, interleukin-6 (IL-6); a potent mediator that is omnipresent in the inflammatory microenvironment of most solid tumors, including glioma [28]. Although previous investigations have confirmed that IL-6 is important in both physiological and pathological angiogenesis [29, 30], IL-6 has recently received more attention as a critical cytokine implicated in the angiogenesis of several human cancers [31–34]. These investigations indicated that IL-6 could induce the release of VEGF from the tumor cells promoting angiogenesis [31–34]. These studies support the notion that IL-6 may be an important regulatory molecule in tumor angiogenesis, but whether it plays a direct role in the process of glioma VM formation is still vague. In this report, we preliminarily explore a correlation of VM with TAMs in human glioma tissues, and the regulatory mechanisms how M2-like macrophages promote VM through amplifying IL-6 secretion in glioma cells.
Correlation of VM level with CD163+ TAM infiltration in human glioma tissue
To investigate whether the density of M2-like TAMs correlated with the VM level in human glioma, we detected the expression of CD163 (a marker of human M2-like TAMs), CD31 and PAS (markers for vessel) in 87 human glioma specimens by immunochemical staining. As shown in Figure 1A, CD163+ cells were present throughout the tumor tissue, and grade IV glioma specimens had the highest number of infiltrated CD163+ cells (Figure 1A). We next explored VM in glioma specimens by CD31/PAS dual staining. The VM (CD31-PAS+) number in high grade was significantly higher compared with low grade or normal brain with edema (Figure 1A, red arrow). As shown in Figure 1B, the levels of VM and infiltrated CD163+cells were positively correlated with glioma grade (P < 0.01; P < 0.01, respectively). More importantly, a correlation of CD31−PAS+ vessels and CD163+ cells was observed in glioma and normal brain tissues. As shown in Figure 1C, VM level is closely associated with CD163+ TAM infiltration (r2 = 0.416, P < 0.001). Taken together, these results clearly revealed a significant positive correlation between VM level and CD163+ TAM infiltration in human glioma tissues.
Figure 1: Correlation of VM level with CD163 expression in human glioma tissue and overall survival for glioma patients. (A) Immunohistochemical staining of CD31/PAS and CD163 in glioma specimens (n = 87). Shown were two representative serial sections (small: 100×; large: 400×). Blood vessel (PAS+CD31+) was shown by black arrow and VM (PAS+CD31−) was shown by red arrow. (B) Numbers of VM and CD163+ cells were both correlated with glioma WHO grade. (C) The correlation between VM counting and CD163+ cell number in each tumor sample. **P < 0.01. (D) Both VM (P < 0.001) and CD163 (P = 0.006) were correlated with overall survival on glioma patients between low and high subgroups. (E) Survival analysis was performed between subgroups combined with VM and CD163. Kaplan–Meier method was used to plot survival curves and statistical significance was determined by log-rank test (P < 0.001).
VM level and CD163 density are associated with survival and clinicopathologic parameters of glioma patients
To determine whether there were prognostically significant association between VM, or CD163 and patient survival, Kaplan-Meier survival curves were then plotted. As shown in Figure 1D, CD163 low group had a significant survival advantage compared with CD163 high group (P = 0.006), and this survival advantage was also shown in VM low group (P < 0.001). We further analyzed patient survival with the combination of CD163 with VM. More significant value was observed in overall patient survival than one single factor (P < 0.001, Figure 1E). The association between VM numbers, CD163+ TAM counts and the clinicopathological status of patients with glioma was then analyzed (Table 1). The number of VM and CD163+ TAMs increased with aggressive tumor biology defined by advanced WHO grade (P < 0.001). VM channels increased with higher tumor burden as defined by tumor size (P < 0.05). No correlation was observed in gender, age, tumor location, KPS and growth pattern of tumor. These data suggested that VM level and the density of CD163+ TAMs were associated with the progression of human glioma.
Table 1: Relationship between expression of CD163, VM and clinicopathological parameters
CD163 expression
VM expression
Low High
Low High
Low High
Low High
> 50
≤ 50
≥ 80
< 80
> 1 lobe
Tumor size (diameter)
> 5cm
≤ 5cm
WHO grade
Gross total resection
Partial resection
M2-like macrophages drive VM formation of glioma cells in vitro
M2-like macrophages was induced by THP-1 cells in vitro. THP-1-derived M2-like macrophages expressed high levels of M2 type markers (CD163, CD206, IL-10, IL-1RA and CCL17) and low levels of M1 type markers (TNF-a, IL-1β and IL-12) (Supplementary Figure S1). To determine the effect of M2-like macrophages on VM formation, tubule formation assays were performed. As shown in Figure 2A and 2B, treatment by conditioned medium from M2-like macrophages (M2-CM) increased approximately 2 fold changes of the tubule structures in U251 cells, compared to THP-1-CM-treated group or control group (P < 0.001, P < 0.001, Figure 2A and 2B). This advantage of the number of tubes was also shown in M2-CM-treated A172 cells (P < 0.01, P < 0.01, Figure 2A and 2B). While there were no significant differences between group cocultured with THP-1-CM and control group in both cells (P > 0.05; P > 0.05, Figure 2A and 2B). The results indicated that M2-like macrophages enhanced the tube formation abilities of glioma cells.
Figure 2: M2-like macrophages drive VM formation of glioma cells in vitro. The glioma cells were incubated in the conditioned medium (CM) from THP-1 or M2 cells for 24 h. The glioma cells cultured in DMEM medium were used as control. (A, B) Representative images (A) and quantification (B) of tubule formation assay on Matrigel matrix in vitro (×100). (C) qRT-PCR was performed to evaluate the effect of THP-1-CM or M2-CM on VM markers. (D) Western blotting was used to detect the expression of VM associated proteins, including MMP-9, MMP-14 and LAMC2. Each bar represents the mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance.
To further examine the roles of M2-like macrophages in VM and to explore the potential mechanisms, we studied VM-related molecular markers previously identified [35]. As shown in Figure 2C, the transcriptional levels of VM markers MMP-9, MMP-14 and LAMC2 were significantly up-regulated after coculture with M2-CM in U251 and A172 cells, while they were not significantly altered in THP-1-CM-treated group (Figure 2C). This upregulation was also verified by western blotting (Figure 2D). Therefore, our data showed that M2-like macrophages promoted the VM formation in glioma cells.
M2-like macrophages amplify IL-6 expression in glioma cells
In the above study, we demonstrated that M2-like macrophages promoted glioma VM. However, the mechanism by which M2-like macrophages promoted glioma VM is still unclear. Therefore, in order to explore the potential target factor and screen the responsible cytokine, cytokine array was performed to analyze conditioned medium from THP-1, M2, U251, THP-1-CM/U251 coculture system and M2-CM/U251 coculture system (Figure 3A). Interestingly, IL-6 production by glioma cells was dramatically increased in the medium of M2-CM/U251 coculture system, compared to other groups (Figure 3A). The upregulation of IL-6 was further confirmed by qRT-PCR and ELISA in U251 cells. To generalize our finding, IL-6 upregulation was also examined in A172 cells. As shown in Figure 3B and 3C, compared to THP-1-CM-treated group, transcription and secretion of IL-6 in glioma cells were markedly increased by M2-CM in dose-dependent manner.
Figure 3: M2-like macrophages amplify IL-6 expression in glioma cells. (A) The Bio-Plex ProTM Human Th17 Cytokine Assay were used to detect the cytokines in the supernatant of THP-1, M2, U251, THP-1-CM/U251 coculture system and M2-CM/U251 coculture system. (B, C) Dose-dependent induction of IL-6 expression in human glioma cells by M2-CM for 24 h. The level of IL-6 mRNA was determined by qRT-PCR (B) and IL-6 concentration in the medium was detected using ELISA assay (C). (D) Time course of IL-6 and VM marker mRNA expression in glioma cells stimulated with M2-CM. Transcript levels were measured at indicated time after stimulation using qRT-PCR. Each bar represents the mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.
To determine the relative timing of IL-6 and VM marker expression following stimulation, mRNA levels of IL-6 and VM markers in glioma cells stimulated with M2-CM were evaluated by qRT-PCR at indicated time points (Figure 3D). IL-6 transcript levels rose first, reaching near-peak expression at 6 hours, whereas VM marker expression lagged behind, rising steadily over 24 hours.
IL-6 upregulation is responsible for VM promotion in glioma cells
In order to determine whether there was a correlation of IL-6 upregulation with VM enhancement in glioma cells, IL-6 neutralizing antibody (anti-IL-6, 1 μg/ml) was used to investigate the effect of IL-6. As shown in Figure 4A and 4B, the number of tubes was significantly decreased after adding anti-IL-6 into the coculture system, and the protein levels of VM markers were abated as well (Figure 4C). In addition, recombinant human IL-6 (rhIL-6, 100 ng/ml) was employed to measure the effect of IL-6. As shown in Figure 4D and 4E, the number of tubes was increased by using rhIL-6 alone compared with their corresponding controls (P < 0.01, P < 0.05), and VM marker expression was augmented correspondingly (Figure 4F). Taken together, these results indicated that IL-6 may play a significant role in M2-CM-enhanced VM in glioma cells.
Figure 4: IL-6 upregulation is responsible for VM promotion in glioma cells. (A, B) Glioma cells were treated with DMEM medium or M2-CM in the presence or absence of anti-IL-6 at 1 μg/ml, or isotype-matched IgG (IgG) control for 24 hours. Representative images (A) and quantification (B) of tubule formation assay on Matrigel matrix in vitro (×100). (C) As previously treatment, the protein levels of VM markers were then detected by Western blotting. (D, E) Glioma cells were treated with rhIL-6 in a concentration of 100 ng/mL or PBS control for 24 hours. Representative images (D) and quantification (E) of tubule formation assay on Matrigel matrix in vitro (×100). (F) VM markers protein of glioma cells, alone or after cultured with rhIL-6. Each bar represents the mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.
M2-like macrophages promote IL-6 and VM in glioma cells via PKC pathway
We went on to investigate the mechanisms in the process of M2-mediated VM promotion. It has been reported that PKC signal pathway played a key role in IL-6 production [36]. To determine whether PKC pathway was involved in our study, we measured the phosphorylation of PKC pathway treated with CM for indicated time points. As shown in Figure 5A, glioma cells were stimulated with M2-CM at different time, and a transient upregulated phosphorylation of PKC pathway was measured by Western blotting. PKC inhibitor Bisindolylmaleimide I significantly inhibited IL-6 transcription (Figure 5B) and secretion (Figure 5C) induced by M2-CM. In addition, VM markers (Figure 5D) and VM formation (Figure 5E) were also inhibited by Bisindolylmaleimide I. However, rhIL-6 significantly rescued the inhibiting effect of Bisindolylmaleimide I on VM in glioma cells cocultured with M2-CM (Figure 5E). In addition, inhibitor of p38 MAP kinase (SB 203580) and inhibitor of phosphatidylinositol 3-kinase (Wortmannin) were also used to examine their effects on IL-6 production, while their significant inhibitory effects were not observed in glioma cells (data not shown). In all, these results indicated that the potent PKC inhibitor bisindolylmaleimide I could prevent M2-induced IL-6 upregulation and further inhibit tubule formation of glioma cells.
Figure 5: M2-enhanced IL-6 and VM in glioma cells via PKC pathway. (A) Glioma cells were incubated with THP-1-CM or M2-CM for indicated time, the phosphorylation of PKC (p-PKC, Pan) was determined by Western blotting. (B, C) Glioma cells were incubated with DMEM medium, M2-CM or M2-CM containing Bisindolylmaleimide I (PKC I) for 24 h, IL-6 transcription (B) and concentration (C) in CM were determined by qRT-PCR and ELISA respectively. (D) As previously treatment, the protein levels of VM markers were then detected by Western blotting. (E) Representative images and quantification of tubule formation assay in glioma cells incubated in M2-CM, M2-CM containing Bisindolylmaleimide I or M2-CM containing Bisindolylmaleimide I with rhIL-6 for 24 h (×100). Each bar represents the mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.
Monocyte-derived M2 macrophages also induce VM promotion via IL-6 amplification in glioma cells
To generalize our findings, we isolated monocytes from human peripheral blood mononuclear cells by magnetic cell sorting using CD14 microbeads. Monocyte-derived M2 macrophages expressed high levels of M2 type markers (CD163, CD206, CCL18 and CCL17) and low levels of M1 type markers (TNF-a, IL-1β, IL-6 and IL-12) ( Supplementary Figure S2). As shown in Figure 6A, monocyte-derived M2 macrophages secreted high levels of M2 type cytokines/chemokines (CCL22, CCL18, CCL17 and IL-10) and low levels of M1 type cytokines (IL1-β, IL-6, IL-12 and IL-8).
Figure 6: Monocyte-derived M2 macrophages also induce VM promotion via IL-6 amplification in glioma cells. (A) The cytokines/chemokines with different concentration secreted by human peripheral blood monocyte-derived M1/M2 macrophages were evaluated by ELISA kit. (B, C) Glioma cells were incubated with DMEM medium, mono-CM or m2-CM for 24 h, IL-6 transcription (B) and concentration (C) in CM were determined by qRT-PCR and ELISA, respectively. (D) Glioma cells were treated with DMEM medium or m2-CM in the presence or absence of anti-IL-6 at 1 μg/ml, or isotype-matched IgG (IgG) control for 24 hours. Representative images and quantification of tubule formation assay on Matrigel matrix in vitro (×100). Each bar represents the mean ± SEM (n = 3). *P<0.05; **P < 0.01; ***P < 0.001; ns, no significance. mono-CM: conditioned medium from CD14+ monocytes. m2-CM: conditioned medium from monocyte-derived M2 macrophages.
In order to determine whether monocyte-derived M2 macrophages could promote VM through IL-6 upregulation in glioma cells, IL-6 transcription and concentration were determined by qRT-PCR and ELISA. As shown in Figure 6B and 6C, IL-6 production by glioma cells was dramatically increased in the medium of monocyte-derived M2-CM/glioma coculture system, compared to other groups. And then IL-6 neutralizing antibody (anti-IL-6, 1 μg/ml) was used to investigate the effect of IL-6. As shown in Figure 6D, the number of tubes was significantly increased after treated by monocyte-derived M2-CM compared to control group (P < 0.01), but the increments were abrogated by anti-IL-6 (P < 0.01). Taken together, monocyte-derived M2 macrophages also induced VM promotion via IL-6 amplification in glioma cells.
Glioblastoma is recognized as a highly angiogenic malignant brain tumor [4]. However, conventional anti-VEGF agents have not significantly extended the life expectancies of glioblastoma patients [6]. The discovery of VM has offered a new horizon for understanding tumor angiogenesis. Meanwhile, it should be noticed the complexity and diversity of the VM-related signal transduction events, including VE-cadherin, EPHA2, Twist 1, COX-2 [37]. However, the molecular mechanisms of glioma VM formation have not been studied explicitly until now. Therefore, in the present study we preliminarily explored the possible mechanisms of this process and provided additional information. Our results showed that both VM and CD163+ TAMs were associated with WHO grade and reduced patient survival, which were consistent with previous studies [13, 19]. After analysis of relevance, we found there was a closely positive correlation between VM level and CD163+ TAMs infiltration, which is a significant phenomenon and implies TAMs may involve in VM formation of glioma (Figure 1).
Recently, accumulating experiments have highlighted the critical role of TAMs during tumor angiogenesis. Previous study confirmed that recurrent glioblastomas showed an increased TAMs infiltration after anti-angiogenic therapy [23], and TAMs promoted angiogenesis in glioma by upregulating the expression of VEGF and pro-inflammatory cytokines that prevented normal vessel formation [38]. VM as an alternative to angiogenesis, hence, glioma VM formation might be influenced by TAMs as well. Moreover, it has been reported that glioma-associated macrophages expressed the elevated M2 cell markers [19]. In order to further examine the effect of TAMs on VM formation, THP-1-derived M2-polarized macrophages (M2-like macrophages) and monocyte-derived M2 macrophages were used as a macrophage model which had a M2 functional profile [39–41]. Data obtained from our study indicated that conditioned medium from M2 cells (M2-CM) increased the tubule formation number in glioma cells, as well as VM marker expression, including LAMC2, MMP-9 and MMP-14 (Figure 2). Previous results have shown that Bevacizumab accelerated metastasis in models of ovarian cancer, with marked VM formation in mice receiving short-term therapy [42]. Therefore, our finding of M2-mediated glioma VM facilitation may be the potential key factor for resistance to conventional anti-angiogenic therapy of glioma.
We further explored possible molecular mechanisms that may be responsible for the proangiogenic effect of M2-like macrophages. Although activated myeloid cells can directly secrete proangiogenic factors [43, 44], TAMs may also regulate tumor VM in an indirect way. Interestingly, in the current study, cytokine array screened the potential key factor IL-6 which was the most significantly upregulated cytokine in glioma cells after stimulation by M2-CM (Figure 3). Recently, a growing number of publications showed that IL-6 was a key player in chemoresistance in various types of malignant states. A study conducted by Conzeet et al. showed that IL-6 and its downstream signaling have been found to be responsible for multidrug resistance [45]. A phase II study suggested that the extent of IL-6 increase in the plasma during treatment was associated with an inferior outcome in patients with rectal and ovarian cancer after bevacizumab and chemoradiation treatment, and an inferior outcome in patients with advanced HCC after sunitinib therapy [46, 47]. Besides, it has been demonstrated that IL-6 promoted VEGF-induced glioma angiogenesis [48, 49]. These results implied that IL-6 may be associated with the resistance to anti-angiogenic therapy in cancer, including glioma. Although the cytokine assay performed in this study did not include all the angiogenic factors secreted by glioma cells, these results in our present study have indicated that M2-CM induced VM enhancement, at least partly via the IL-6 amplification in glioma cells. These findings indicated another relevant mechanism of resistance to anti-angiogenic therapy and may aid in the identification of new targets for combinatorial antiangiogenic therapy.
The M2-CM facilitated the IL-6 production and VM formation of glioma cells via PKC pathway compared to THP-1-CM, and the facilitation was mostly abrogated by inhibition of PKC signaling (Figure 5). The results indicated that the potent PKC inhibitor could prevent IL-6 upregulation and further destroy tubule formation of glioma cells. PKC is a family of phospholipid dependent serine/threonine kinases that function in numerous different cell types. In astrocytes, PKC was previously shown to be important for IL-6 induction [36]. Since PKC activation can be a very fast process, sometimes disappearing already after a few minutes [50], and PKC could be invoked in our experimental system ranged from approximately 5 min to 3 h, it became virtually impossible to identify the responsible PKC isoforms. In view of the complexity of the PKC pathway, the specific mechanisms which M2-CM promoted IL-6 expression and VM formation in glioma cells need further investigation by isoform-specific inhibitors.
In summary, these data indicated that VM level was positively correlated with the number of infiltrated CD163+ M2-like TAMs in glioma specimens. The in vitro studies suggested that M2-like macrophages and monocyte-derived M2 macrophages drove VM formation through upregulating IL-6 expression in glioma cells via PKC pathway. A schematic diagram was made to depict the detailed mechanisms (Figure 7). Nevertheless, other angiogenic and anti-angiogenic peptides produced by TAMs and glioma cells may also contribute to tumor angiogenesis via different signaling pathways. Thus, further investigations are needed to define the optimal targets for treatment of glioma patients.
Figure 7: The schematic diagram depicting M2-like tumor-associated macrophages driving VM formation through amplification of IL-6 expression in glioma cells via PKC signaling.
Patients and samples
Tissue samples from 87 patients diagnosed with glioma were obtained during the surgical tumor resection at the Department of Neurosurgery, Qilu Hospital of Shandong University. None of the patients had received preoperative adjuvant therapy and KPS score was performed before surgery. The glioma specimens were classified according to the WHO classification criteria and verified by two experienced pathologists. The four normal brain tissues were collected from patients with hernia during surgical decompression. This study was in accordance with the Declaration of Helsinki, and written informed consent was obtained from each patient. The Ethics Committees of Qilu Hospital of Shandong University approved the experiments.
Reagents and antibodies
Isotype-IgG, rhIL-4, rhIL-13, M-CSF, rhIL-6, anti-IL-6 and human IL-6 Quantikine ELISA were purchased from R&D Systems. Phorbol 12-myristate 13-acetate (PMA) was purchased from Sigma. Bisindolylmaleimide I (protein kinase C (PKC) inhibitor) was purchased from Calbiochem. Matrigel was from BD Biosciences. The primary human antibodies in this study were used: mouse anti-CD163, rabbit anti-CD31 (ZSGB-BIO); rabbit anti-MMP14, rabbit anti-LAMC2 (Abcam); rabbit anti-MMP9, rabbit anti-Phosphorylated PKC (pan) (Cell Signaling Technology); mouse anti-β-actin (Beyotime).
Immunohistochemical staining
Paraffin sections (4-μm thick) of glioma tissue specimens were prepared for immunohistochemistry. Briefly, the sections were deparaffinized, rehydrated, boiled in 1 mM EDTA (pH 8.0) for antigen retrieval and blocked with goat serum for endogenous peroxidase activity to reduce nonspecific staining. After the incubation with primary antibodies against CD163 at 4°C overnight, the specimens were then incubated with poly-HRP secondary antibodies for 30 min. Finaly, the sections were stained with diaminobenzidine and the nuclei were counterstained with hematoxylin. Images were captured using an Olympus IX81 microscope.
The specimens were assessed by two independent pathologists without knowing any clinicopathologic variables. For CD163 evaluation in glioma samples, the five representative areas (hot spots) were identified by scanning the whole tumor section at low power, followed by counting positive staining cells in each hot spot at 400× magnification, and the mean number per section was obtained. Then the mean number of CD163+ cells infiltrated in glioma samples was calculated (21.9 per field). A mean number < 22 was considered low, a mean number ≥ 22 was considered high.
CD31/Periodic acid-Schiff doublestaining
After immunohistochemical analysis of sections for CD31 expression, the sections were exposed to 1% sodium periodate for 10 min, rinsed for 5 min with distilled water, and then incubated for 15 min with PAS in dark at 37°C. The sections were counterstained with hematoxylin and observed using a microscope. CD31 positive vessels indicated blood vessels in tissues, and CD31 negative, PAS positive vessels were defined as VM. We evaluated VM expression in tumor specimens according to the previous protocols. The CD31/PAS dual-staining sections were viewed at 400× magnification using an Olympus IX81 microscope. The mean VM count of five hot spots was calculated as the VM density (VMD) for each section. The average VMD from all glioma sections was calculated (1.5 per field). All cases with the number < 1.5 per field were considered low, the number ≥ 1.5 considered high.
Cell culture and treatments
Human malignant glioma cell lines U251, A172 and human monocytic cell line THP-1 were obtained from the Cell Bank of Type Culture Collection of Chinese Academy of Science (CBTCCCAS; Shanghai, China). The cell lines had been recently authenticated based on DNA fingerprinting, isozyme detection and cross species checks. All cells were maintained in suspension in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified incubator with 95% air, 5% CO2.
To generate THP-1-derived M2-polarized macrophages (M2 cells), THP-1 cells (in a six-well plate, 1 × 106 cells/well) were treated with 100 ng/ml PMA for 6 h, and then cultured with PMA plus 20 ng/ml IL-4 and 20 ng/ml IL-13 for another 18 h. For production of the conditioned medium from M2 cells (M2-CM), all PMA, IL-4 and IL-13 were removed by a thorough wash, and M2 cells were further cultured in 2 ml fresh serum-free DMEM medium for another 24 h. In order to prepare conditioned medium from THP-1 cells (THP-1-CM), THP-1 cells were cultured in 2 ml fresh medium for 24 h. Then the conditioned medium were collected respectively and stored at −80°C.
Human peripheral blood monocytes (PBMs) from healthy donors were isolated by density-gradient centrifugation using Ficoll-Hypaque (Pharmacia, Peapack). Monocytes were isolated using anti-CD14 microbeads (Miltenyi Biotec), and more than 97% of the cells acquired were CD14+ monocytes as determined by FACS analysis (data not shown). For in-vitro monocyte-derived M2 macrophages activation, CD14+ monocytes at 1 × 106 cells/mL were treated for 5 days with 100 ng/ml recombinant human macrophage colony-stimulating factor, followed by 20 ng/ml IL-4 and 20 ng/ml IL-13 for 3 days. This study was approved by the institutional review board of Qilu Hospital of Shandong University. Informed consent was obtained from each subject.
Tubule formation assay for VM
VM was evaluated using a three-dimensional (3D) culture model containing growth factor-reduced Matrigel (BD Biosciences). Briefly, a volume of 50 μL growth factor-reduced Matrigel was plated in 96-well plates, and allowed to polymerize at 37°C for 30 minutes. Next, 2 × 104 cells were trypsinized and resuspended with serum-free medium, and then seeded onto the Matrigel layer. After 9 h incubation at 37°C with 5% CO2, each well was captured directly by an Olympus BX61 fluorescence microscope under a phase-contrast microscope (×100). The images were quantified for the mean area value of randomly selected vascular network meshes by ImageJ software. Each experiment was performed in triplicate.
RNA extraction and quantitative real-time PCR
Total RNA was extracted from cell lines using Trizol reagent (Invitrogen), and reverse transcriptase reactions were carried out by ReverTra Ace qPCR RT Kit (Toyobo) method in accordance with the manufacturer's instructions to generate cDNA. Quantitative real-time PCR (qRT-PCR) was performed using a SYBR Green Master Mix kit (Toyobo) on the LightCycler 2.0 instrument (Roche Applied Science). mRNA levels were normalized to GAPDH. The primer sequences used in this study were listed in Supplementary Table S1. The absolute expression levels were calculated as concentration ratios using a Roche LightCycler® 2.0 system.
Western blotting
Total protein was extracted from cells using RIPA buffer containing 1% phenylmethylsulfonyl fluoride (PMSF). The protein concentration in the medium was determined using a BCA Protein Assay Kit (Beyotime). Equal amounts of total protein were subjected to electrophoresis in 10% SDS-polyacrylamide gels and then transferred onto polyvinylidene difluoride membranes. The membranes were blocked by 5% skim milk blocking buffer for 1 hour. Next, blots were incubated with the primary antibody against MMP-9 (1:1,000; CST), LAMC2 (1:1,000; CST), pPKC (1:1,000; CST), β-actin (1:1,000; Beyotime), or MMP-14 (1:1,000; Abcam). The membranes were then incubated with horseradish peroxidase-linked secondary anti-rabbit or anti-mouse antibodies (1:5,000; Beyotime). Finally, the protein bands were visualized using enhanced chemiluminescence (ECL) assay (Thermo).
Cytokine array
Cytokine array was used to detect the level of cytokines or chemokines in the medium of glioma cells with or without treatment. Glioma cells were seeded in 6-well plates at a density of 2 × 105 cells per well. After incubation for 24 hours, cells were washed with PBS three times and cultured in fresh serum-free medium, THP-1-CM and M2-CM, respectively. After 24 h, the conditioned medium was collected and centrifuged to remove cell debris, and the mediums were frozen at −80°C until further analysis by cytokine array assay. The Bio-Plex ProTM Human Th17 Cytokine Assay was used in this study in accordance with the manufacturer's instructions.
Enzymes linked immunosorbent assay (ELISA)
IL-1β, IL-10, IL-12, IL-8, CCL22, CCL18, CCL17 and IL-6 levels in culture mediums were measured using the human ELISA Kit (R&D Systems) in accordance with the manufacture's instruction.
Statistical analysis
All statistical analysis and experimental graphs were performed using SPSS 13.0 (SPSS, IL, USA) and GraphPad Prism 5 (GraphPad, CA, USA). The relationship between CD163 and VM was examined by Spearman analysis, and the differences of clinicopathological variables were analyzed with χ2-test. Statistical comparisons among groups were made by Student's t test and ANOVA. Data were shown as the mean ± standard error of the mean (SEM), and all experiments were repeated independently at least three times. P < 0.05 was considered statistically significant.
We thank members of the Xingang Li laboratory for their helpful discussions.
No potential conflicts of interest were disclosed by the authors.
This work was supported by grants from the National Natural Science Foundation of China (No.31470885, No.31300752, No.31270971, No. 81300510, No.30901326, No.81072406, No.81301791, No.81501761, and No.81572487).
1. Omuro A, DeAngelis LM. Glioblastoma and other malignant gliomas: a clinical review. JAMA. 2013; 310:1842–50. doi: 10.1001/jama.2013.280319.
2. Brandes AA, Tosoni A, Franceschi E, Reni M, Gatta G, Vecht C. Glioblastoma in adults. Crit Rev Oncol Hematol. 2008; 67:139–52. doi: 10.1016/j.critrevonc.2008.02.005.
3. Stupp R, Mason WP, van den Bent MJ, Weller M, Fisher B, Taphoorn MJ, Belanger K, Brandes AA, Marosi C, Bogdahn U, Curschmann J, Janzer RC, Ludwin SK, et al. Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma. N Engl J Med. 2005; 352:987–96. doi: 10.1056/NEJMoa043330.
4. Leon SP, Folkerth RD, Black PM. Microvessel density is a prognostic indicator for patients with astroglial brain tumors. Cancer. 1996; 77: 362–72. doi: 10.1002/(SICI)1097-0142(19960115)77:2<362::AID-CNCR20>3.0.CO;2-Z.
5. Vredenburgh JJ, Desjardins A, Herndon JE, 2nd, Dowell JM, Reardon DA, Quinn JA, Rich JN, Sathornsumetee S, Gururangan S, Wagner M, Bigner DD, Friedman AH, Friedman HS. Phase II trial of bevacizumab and irinotecan in recurrent malignant glioma. Clin Cancer Res. 2007; 13:1253–9. doi: 10.1158/1078-0432.CCR-06-2309.
6. Sennino B, McDonald DM. Controlling escape from angiogenesis inhibitors. Nat Rev Cancer. 2012; 12:699–709. doi: 10.1038/nrc3366.
7. Bergers G, Hanahan D. Modes of resistance to anti-angiogenic therapy. Nat Rev Cancer. 2008; 8:592–603. doi: 10.1038/nrc2442.
8. Ebos JM, Lee CR, Cruz-Munoz W, Bjarnason GA, Christensen JG, Kerbel RS. Accelerated metastasis after short-term treatment with a potent inhibitor of tumor angiogenesis. Cancer Cell. 2009; 15:232–9. doi: 10.1016/j.ccr.2009.01.021.
9. Paez-Ribes M, Allen E, Hudock J, Takeda T, Okuyama H, Vinals F, Inoue M, Bergers G, Hanahan D, Casanovas O. Antiangiogenic therapy elicits malignant progression of tumors to increased local invasion and distant metastasis. Cancer Cell. 2009; 15:220–31. doi: 10.1016/j.ccr.2009.01.027.
10. Maniotis AJ, Folberg R, Hess A, Seftor EA, Gardner LM, Pe'er J, Trent JM, Meltzer PS, Hendrix MJ. Vascular channel formation by human melanoma cells in vivo and in vitro: vasculogenic mimicry. Am J Pathol. 1999; 155:739–52. doi: 10.1016/S0002-9440(10)65173-5.
11. Baeten CI, Hillen F, Pauwels P, de Bruine AP, Baeten CG. Prognostic role of vasculogenic mimicry in colorectal cancer. Dis Colon Rectum. 2009; 52:2028–35. doi: 10.1007/DCR.0b013e3181beb4ff.
12. Liu WB, Xu GL, Jia WD, Li JS, Ma JL, Chen K, Wang ZH, Ge YS, Ren WH, Yu JH, Wang W, Wang XJ. Prognostic significance and mechanisms of patterned matrix vasculogenic mimicry in hepatocellular carcinoma. Med Oncol. 2011; 28:S228–38. doi: 10.1007/s12032-010-9706-x.
13. Liu XM, Zhang QP, Mu YG, Zhang XH, Sai K, Pang JC, Ng HK, Chen ZP. Clinical significance of vasculogenic mimicry in human gliomas. J Neurooncol. 2011; 105:173–9. doi: 10.1007/s11060-011-0578-5.
14. Kirschmann DA, Seftor EA, Hardy KM, Seftor RE, Hendrix MJ. Molecular pathways: vasculogenic mimicry in tumor cells: diagnostic and therapeutic implications. Clin Cancer Res. 2012; 18:2726–32. doi: 10.1158/1078-0432.CCR-11-3237.
15. Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011; 144:646–74. doi: 10.1016/j.cell.2011.02.013.
16. Solinas G, Germano G, Mantovani A, Allavena P. Tumor-associated macrophages (TAM) as major players of the cancer-related inflammation. J Leukoc Biol. 2009; 86:1065– 73. doi: 10.1189/jlb.0609385.
17. Roggendorf W, Strupp S, Paulus W. Distribution and characterization of microglia/macrophages in human brain tumors. Acta Neuropathol. 1996; 92:288–93. doi:
18. Shinonaga M, Chang CC, Suzuki N, Sato M, Kuwabara T. Immunohistological evaluation of macrophage infiltrates in brain tumors. Correlation with peritumoral edema. J Neurosurg. 1988; 68:259–65. doi: 10.3171/jns.1988.68.2.0259.
19. Prosniak M, Harshyne LA, Andrews DW, Kenyon LC, Bedelbaeva K, Apanasovich TV, Heber-Katz E, Curtis MT, Cotzia P, Hooper DC. Glioma grade is associated with the accumulation and activity of cells bearing M2 monocyte markers. Clin Cancer Res. 2013; 19:3776–86. doi: 10.1158/1078-0432.CCR-12-1940.
20. Lin L, Chen YS, Yao YD, Chen JQ, Chen JN, Huang SY, Zeng YJ, Yao HR, Zeng SH, Fu YS, Song EW. CCL18 from tumor-associated macrophages promotes angiogenesis in breast cancer. Oncotarget. 2015; 6:34758–73. doi: 10.18632/oncotarget.5325.
21. Tjiu JW, Chen JS, Shun CT, Lin SJ, Liao YH, Chu CY, Tsai TF, Chiu HC, Dai YS, Inoue H, Yang PC, Kuo ML, Jee SH. Tumor-associated macrophage-induced invasion and angiogenesis of human basal cell carcinoma cells by cyclooxygenase-2 induction. J Invest Dermatol. 2009; 129:1016–25. doi: 10.1038/jid.2008.310.
22. Rivera LB, Meyronet D, Hervieu V, Frederick MJ, Bergsland E, Bergers G. Intratumoral myeloid cells regulate responsiveness and resistance to antiangiogenic therapy. Cell Rep. 2015; 11:577–91. doi: 10.1016/j.celrep.2015.03.055.
23. Lu-Emerson C, Snuderl M, Kirkpatrick ND, Goveia J, Davidson C, Huang Y, Riedemann L, Taylor J, Ivy P, Duda DG, Ancukiewicz M, Plotkin SR, Chi AS, et al. Increase in tumor-associated macrophages after antiangiogenic therapy is associated with poor survival among patients with recurrent glioblastoma. Neuro Oncol. 2013; 15:1079–87. doi: 10.1093/neuonc/not082.
24. Lippitz BE. Cytokine patterns in patients with cancer: a systematic review. Lancet Oncol. 2013; 14:e218–28. doi: 10.1016/S1470-2045(12)70582-X.
25. Sarvaiya PJ, Guo D, Ulasov I, Gabikian P, Lesniak MS. Chemokines in tumor progression and metastasis. Oncotarget. 2013; 4:2171–85. doi: 10.18632/oncotarget.1426.
26. Amedei A, Prisco D, MM DE. The use of cytokines and chemokines in the cancer immunotherapy. Recent Pat Anticancer Drug Discov. 2013; 8:126–42. doi:
27. Sun J, Feng A, Chen S, Zhang Y, Xie Q, Yang M, Shao Q, Liu J, Yang Q, Kong B, Qu X. Osteopontin splice variants expressed by breast tumors regulate monocyte activation via MCP-1 and TGF-beta1. Cell Mol Immunol. 2013; 10:176–82. doi: 10.1038/cmi.2012.67.
28. Guo Y, Xu F, Lu T, Duan Z, Zhang Z. Interleukin-6 signaling pathway in targeted therapy for cancer. Cancer Treat Rev. 2012; 38:904–10. doi: 10.1016/j.ctrv.2012.04.007.
29. Cohen T, Nahari D, Cerem LW, Neufeld G, Levi BZ. Interleukin 6 induces the expression of vascular endothelial growth factor. J Biol Chem. 1996; 271:736–41. doi:
30. Mahnke JL, Dawood MY, Huang JC. Vascular endothelial growth factor and interleukin-6 in peritoneal fluid of women with endometriosis. Fertil Steril. 2000; 73:166–70. doi:
31. Wei LH, Kuo ML, Chen CA, Chou CH, Lai KB, Lee CN, Hsieh CY. Interleukin-6 promotes cervical tumor growth by VEGF-dependent angiogenesis via a STAT3 pathway. Oncogene. 2003; 22:1517–27. doi: 10.1038/sj.onc.1206226.
32. Jee SH, Chu CY, Chiu HC, Huang YL, Tsai WL, Liao YH, Kuo ML. Interleukin-6 induced basic fibroblast growth factor-dependent angiogenesis in basal cell carcinoma cell line via JAK/STAT3 and PI3-kinase/Akt pathways. J Invest Dermatol. 2004; 123:1169–75. doi: 10.1111/j.0022-202X.2004.23497.x.
33. Adachi Y, Aoki C, Yoshio-Hoshino N, Takayama K, Curiel DT, Nishimoto N. Interleukin-6 induces both cell growth and VEGF production in malignant mesotheliomas. Int J Cancer. 2006; 119:1303–11. doi: 10.1002/ijc.22006.
34. Huang SP, Wu MS, Shun CT, Wang HP, Lin MT, Kuo ML, Lin JT. Interleukin-6 increases vascular endothelial growth factor and angiogenesis in gastric carcinoma. J Biomed Sci. 2004; 11:517–27. doi: 10.1159/000077902.
35. Paulis YW, Soetekouw PM, Verheul HM, Tjan-Heijnen VC, Griffioen AW. Signalling pathways in vasculogenic mimicry. Biochim Biophys Acta. 2010; 1806:18–28. doi: 10.1016/j.bbcan.2010.01.001.
36. Norris JG, Tang LP, Sparacio SM, Benveniste EN. Signal transduction pathways mediating astrocyte IL-6 induction by IL-1 beta and tumor necrosis factor-alpha. J Immunol. 1994; 152:841–50. doi:
37. Seftor RE, Hess AR, Seftor EA, Kirschmann DA, Hardy KM, Margaryan NV, Hendrix MJ. Tumor cell vasculogenic mimicry: from controversy to therapeutic promise. Am J Pathol. 2012; 181:1115–25. doi: 10.1016/j.ajpath.2012.07.013.
38. Chen X, Zhang L, Zhang IY, Liang J, Wang H, Ouyang M, Wu S, da Fonseca AC, Weng L, Yamamoto Y, Yamamoto H, Natarajan R, Badie B. RAGE expression in tumor-associated macrophages promotes angiogenesis in glioma. Cancer Res. 2014; 74:7285–97. doi: 10.1158/0008-5472.CAN-14-1240.
39. Chen JJ, Yao PL, Yuan A, Hong TM, Shun CT, Kuo ML, Lee YC, Yang PC. Up-regulation of tumor interleukin-8 expression by infiltrating macrophages: its correlation with tumor angiogenesis and patient survival in non-small cell lung cancer. Clin Cancer Res. 2003; 9:729–37. doi:
40. Chen JJ, Lin YC, Yao PL, Yuan A, Chen HY, Shun CT, Tsai MF, Chen CH, Yang PC. Tumor-associated macrophages: the double-edged sword in cancer progression. J Clin Oncol. 2005; 23:953–64. doi: 10.1200/JCO.2005.12.172.
41. Chen J, Yao Y, Gong C, Yu F, Su S, Chen J, Liu B, Deng H, Wang F, Lin L, Yao H, Su F, Anderson KS, et al. CCL18 from tumor-associated macrophages promotes breast cancer metastasis via PITPNM3. Cancer Cell. 2011; 19:541–55. doi: 10.1016/j.ccr.2011.02.006.
42. Xu Y, Li Q, Li XY, Yang QY, Xu WW, Liu GL. Short-term anti-vascular endothelial growth factor treatment elicits vasculogenic mimicry formation of tumors to accelerate metastasis. J Exp Clin Cancer Res. 2012; 31:16. doi: 10.1186/1756-9966-31-16.
43. Coussens LM, Tinkle CL, Hanahan D, Werb Z. MMP-9 supplied by bone marrow-derived cells contributes to skin carcinogenesis. Cell. 2000; 103:481–90. doi:
44. Bergers G, Brekken R, McMahon G, Vu TH, Itoh T, Tamaki K, Tanzawa K, Thorpe P, Itohara S, Werb Z, Hanahan D. Matrix metalloproteinase-9 triggers the angiogenic switch during carcinogenesis. Nat Cell Biol. 2000; 2:737–44. doi: 10.1038/35036374.
45. Conze D, Weiss L, Regen PS, Bhushan A, Weaver D, Johnson P, Rincon M. Autocrine production of interleukin 6 causes multidrug resistance in breast cancer cells. Cancer Res. 2001; 61:8851–8. doi:
46. Willett CG, Duda DG, di Tomaso E, Boucher Y, Ancukiewicz M, Sahani DV, Lahdenranta J, Chung DC, Fischman AJ, Lauwers GY, Shellito P, Czito BG, Wong TZ, et al. Efficacy, safety, and biomarkers of neoadjuvant bevacizumab, radiation therapy, and fluorouracil in rectal cancer: a multidisciplinary phase II study. J Clin Oncol. 2009; 27:3020–6. doi: 10.1200/JCO.2008.21.1771.
47. Zhu AX, Sahani DV, Duda DG, di Tomaso E, Ancukiewicz M, Catalano OA, Sindhwani V, Blaszkowsky LS, Yoon SS, Lahdenranta J, Bhargava P, Meyerhardt J, Clark JW, et al. Efficacy, safety, and potential biomarkers of sunitinib monotherapy in advanced hepatocellular carcinoma: a phase II study. J Clin Oncol. 2009; 27:3027–35. doi: 10.1200/JCO.2008.20.9908.
48. Loeffler S, Fayard B, Weis J, Weissenberger J. Interleukin-6 induces transcriptional activation of vascular endothelial growth factor (VEGF) in astrocytes in vivo and regulates VEGF promoter activity in glioblastoma cells via direct interaction between STAT3 and Sp1. Int J Cancer. 2005; 115:202–13. doi: 10.1002/ijc.20871.
49. Saidi A, Hagedorn M, Allain N, Verpelli C, Sala C, Bello L, Bikfalvi A, Javerzat S. Combined targeting of interleukin-6 and vascular endothelial growth factor potently inhibits glioma growth and invasiveness. Int J Cancer. 2009; 125:1054–64. doi: 10.1002/ijc.24380.
50. Ohmori S, Shirai Y, Sakai N, Fujii M, Konishi H, Kikkawa U, Saito N. Three distinct mechanisms for translocation and activation of the delta subspecies of protein kinase C. Mol Cell Biol. 1998; 18:5263–71. doi:10.1128/MCB.18.9.5263.
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I'm often caught up in a pattern with clients.
They'll come to me and ask me to help them either start on their agile journeys or improve / accelerate their current efforts.
But then, when the actual logistics are discussed, we try to minimize everything. That is coaching and training time. The primary two reasons are budget and the time investment. I guess folks are focused on getting the max for the minimum. (sounds like a department store doesn't it?) https://m.tjmaxx.tjx.com
So, I keep reducing my recommendations and approaches until at some point it fits the budget and time tolerances. But often that comes at a cost. 10 years ago, I would minimize to the point where the results would be impacted, but I try not to do that anymore.
Now, I'm much better at holding the line. At negotiating, by keeping their needs and the ultimate outcome in mind. At keeping everyone focused on the goals.
So, where are you going with this, Bob?
Asking for the Moon
I came upon this article by Joshua Kerievsky. In it he makes the point that we should try to begin our agile journeys by…Asking for the Moon.
That is, maximizing our ask in an effort to really set ourselves up for success. From the beginning. And avoiding succumbing to pressure and our minimus tendencies.
So, I ask you to have the courage to consider asking for the moon. But be prepared, you just might get it…
Stay agile my friends,


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