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Original Research ARTICLE
Expression of ABCG2 (BCRP) in mouse models with enhanced erythropoiesis
- 1Diabetes and Nutritional Sciences Division, School of Medicine, King's College London, London, UK
- 2Vascular Sciences Unit, Imperial Centre for Translational and Experimental Medicine, Imperial College, NHLI, London, UK
- 3Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand
Haem is a structural component of numerous cellular proteins which contributes significantly to iron metabolic processes in mammals but its toxicity demands that cellular levels must be tightly regulated. Breast Cancer Resistance Protein (BCRP/ABCG2), an ATP Binding Cassette G-member protein has been shown to possess porphyrin/haem efflux function. The current study evaluated the expression and regulation of Abcg2 mRNA and protein levels in mouse tissues involved in erythropoiesis. Abcg2 mRNA expression was enhanced in bone marrow hemopoietic progenitor cells from mice that were treated with phenylhydrazine (PHZ). Abcg2 mRNA expression was increased particularly in the extramedullary haematopoietic tissues from all the mice models with enhanced erythropoiesis. Haem oxygenase (ho1) levels tended to increase in the liver of mice with enhanced erythropoiesis and gene expression patterns differed from those observed in the spleen. Efflux of haem biosynthetic metabolites might be dependent on the relative abundance of Abcg2 or ho1 during erythropoiesis. Abcg2 appears to act principally as a safety valve regulating porphyrin levels during the early stages of erythropoiesis and its role in systemic haem metabolism and erythrophagocytosis, in particular, awaits further clarification.
Iron turnover in mammals is largely explained by the production (biosynthesis) and breakdown (biodegradation) of erythrocytes. Consequently iron fluxes during erythropoiesis and erythrophagocytosis must be balanced and this is mainly achieved by influences of hepcidin in the maintenance of iron homeostasis. Three essential substrates, iron, porphyrin, and haem as well as their combination, are essential for erythropoiesis but are potentially toxic if allowed to accumulate. Iron, haem, and photosensitized porphyrin are pro-oxidants and they can cause damage to DNA, proteins and cell membrane systems. Availability of iron, a limiting nutrient, and the de novo synthesis of haem and porphyrin are therefore strictly coordinated and channeled to avert the accumulation of excess toxic metabolites. Recently the identification of two haem efflux proteins, Breast Cancer Resistant Protein (BCRP or ABCG2) and Feline Leukaemia Virus C Receptor (FLVCR) has defined important mechanisms that protect cells and tissues against noxious free haem (Quigley et al., 2004; Krishnamurthy and Schuetz, 2005). Abcg2 is a half ATP-Binding Cassette (ABC) G-member transporter with a nucleotide binding domain (NBD) at its amino terminus and a transmembrane domain at its carboxyl end (Krishnamurthy and Schuetz, 2006). It functions conventionally as a plasma membrane protein extruding endogenous and exogenous toxic xenobiotics from the intestine, liver, placenta, and the blood/brain barrier. This transporter, in some instances, confers resistance to anticancer drugs. The broad substrate spectrum of abcg2 are porphyrin metabolites (Suzinges-Mandon et al., 2010). Specifically, Abcg2 was shown as a porphyrin efflux protein which functions to rid erythroid cells of excess porphyrins particularly under hypoxic conditions (Krishnamurthy et al., 2004) and thereby protects haematopoietic cells from the potential toxicity of excess free porphyrin compounds. As hypoxia could vary in magnitude depending on the duration and the type of anaemia, the detoxification function of abcg2 might be pertinent in the various other tissues that handle large quantities of haem and its degradation products e.g., liver.
At the cellular level, haem oxygenase 1 (ho1) catalyses the rate limiting step in the degradation of haem into iron, carbon monoxide, and biliverdin, which is reduced by biliverdin reductase to bilirubin. These by-products also constitute potential substrates for abcg2, and have recently been shown to have antioxidant, anti-inflammatory, and cytoprotective functions (Bilban et al., 2008; Soares and Bach, 2009). Excess haem is presumed to be channeled into the lysosome for catabolism or might be effluxed into circulation where it binds to hemopexin (Tolosano et al., 2010). Haem and its metabolites may be substantially and strategically trafficked or otherwise degraded in a coordinated and regulated manner under different modulators of iron metabolism. Moreover, abcg2 might play a vital role in the transport of haem and porphyrin compounds during erythropoiesis. This study investigates the expression and regulation of abcg2 and ho1 in tissues of mice with enhanced erythropoiesis.
Materials and Methods
Animals and Tissue Collection
CD1 mice were used for all experiments except the hypotransferrinaemic (Hpx) mice that are of Balb/c background. Mice were placed in a hypobaric chamber at 0.5 atm for 72 h to effect hypoxia. Controls were kept at room air pressure. Dietary iron deficiency was induced by feeding 4-week old CD1 male mice with a low-iron diet (Formula TD. 80396, elemental iron concentration: 3–6 mg/kg or control diet containing 48 mg/kg iron, TD. 80394; Harlan-Teklad; Madison, WI, USA) for 3 weeks. Enhanced erythrocyte turnover was induced by intraperitoneal injections of neutralized phenylhydrazine (PHZ) at 60 mg/kg body weight; (Sigma-Aldrich, UK) on 2 consecutive days. All mice were maintained on standard commercial diet (Rodent Maintenance diet; RM1, Special Diet Services, UK) feed and water ad libitum. Mice were sacrificed after anaesthesia and neck dislocation, after which tissues were collected. Femurs were excised and flushed with Dulbecco's minimum essential medium (DMEM; Sigma-Aldrich, UK), to collect the hemopoeitic progenitor cells from the bone marrow of control and PHZ-treated CD1 mice. All procedures were approved and conducted in accordance with the UK. Animals (Scientific Procedures Act, 1986).
Cell Culture
Total RNA was extracted from tissue samples using Trizol reagent (Invitrogen, UK) according to manufacturer's instructions. Quantitative RT-PCR was carried out using an ABI Prism 7000 detection system in a two-step protocol with SYBR Green (ABI, Life Technologies, UK). The efficacy of the amplification was confirmed by a melting curve analysis and gel electrophoresis to confirm the presence of a single product.
Quantitative measurement of each gene was derived from a standard curve constructed from known amounts of PCR product. The results were calculated by the ΔCt method that expresses the difference in threshold for the target gene relative to that of 18S RNA. Sequences of primers used, forward, and reverse, respectively, are as follow:
Mouse abcg2 5′-TCGCAGAAGGAGATGTGTTGAG-3′
Mouse tfr1, 5′-CATGGTGACCATAGTGCACTCA-3′
Mouse ho1 5′-CAAGGAGGTACACATCCAAGCC-3′
Mouse 18s 5′-GAATTCCCAGTAAGTGCGGG-3′
Human 18s 5′-AACTTTCGATGGTAGTCGCCG-3′
Western blot analysis
Spleen tissue was homogenized (in a buffer containing 50 mM mannitol, 2 mM Hepes, 0·5 mM PMSF and pH 7·2) with an Ultra Turrax (IKA, Staufen, Germany) homogenizer in (3 × 30 s pulses at full speed). The homogenate was centrifuged at 1500 g for 5 min and the supernatant was centrifuged for 1 h at 15,000 g to obtain the crude membrane fraction. Protein concentration was determined using Bio-Rad reagents (Bio-Rad, Laboratories, Hercules, CA, USA). Fifty (50) μg of membrane extracts were loaded onto a 12% gel in a SDS-PAGE. The proteins separated were then transferred to Hybond ECL-nitrocellulose membrane (Amersham Biosciences, Bucks, UK) using a Bio-Rad semidry transfer apparatus (Trans-BlotR SD Semi-Dry Transfer Cell; Bio-Rad, UK). Membranes were blocked with 5% milk for 1 h and probed with ABCG2 BXP-53 monoclonal (Santa Cruz Biotechnology, USA), and, β-actin (Sigma, UK) antibodies diluted in 0.01% milk in TBS. Cross-reactivity was observed with peroxidase-linked anti-IgG by using SuperSignal West Pico (Thermo Scientific, USA).
Statistical analysis
All values are expressed as mean ± s.e.m. Statistical differences between means were calculated with Microsoft Excel 6.0 (Microsoft, Seattle, WA, USA) by using the Student t-test correcting for differences in sample variance. When multiple comparisons were necessary, One-Way analysis of variance (ANOVA) was performed using SPSS 14 (SPSS Inc, Chicago, US) with Tukey's post-hoc test.
Abcg2 and tfr1 mRNA expression were enhanced in bone marrow cells from mice treated with PHZ (Figure 1). Increased erythropoietic activity, which typifies Hpx, PHZ-treated, hypoxic, and iron-deficient mice, was associated with significant increases in abcg2 mRNA expression in the spleen tissues from the mice (Figure 2). While changes in ho1 mRNA expression in spleen tissues varied widely with the mice models (Figure 3A), hepatic ho1 mRNA was induced significantly in Hpx and PHZ-treated mice (Figure 3B) instead. Enhancement of abcg2 protein expression in the spleen from mice with enhanced erythropoiesis correlated with the pattern of increased mRNA expression (Figures 4A,B). There was a slight, but observable, increase in ho1 protein levels in spleen tissues from PHZ-treated, hypoxic, and iron-deficient mice (Figures 4A,C).
Figure 1. Abcg2 and TfR1 mRNA levels in bone marrow precursor cells of CD1 mice. Erythropoiesis was induced with phenylhydrazine (PHZ) administration (60 mg/kg) over 2 days. Relative mRNA expression was obtained by normalizing mRNA levels to 18S mRNA. Analysis by Students' T-test showed significant effects of abcg2 (P < 0.01), and TfR1 (P < 0.005) over the control samples.
Figure 2. Quantitative Real-Time PCR of Abcg2 mRNA levels in spleen of mouse models with enhanced erythropoiesis. Abcg2 mRNA levels in the spleen of Hpx, PHZ-treated, hypoxic, and iron-deficient mice. Data are mean ± s.e.m. of four observations in each group and are expressed as a ratio of 18S mRNA in arbitrary units. Analysis by Students' T-test showed significant effects of abcg2 mRNA in Hpx (P < 0.01), PHZ (P < 0.01), hypoxia (P < 0.05), and iron-deficient spleen tissues, (P < 0.05) over the control samples.
Figure 3. Quantitative Real-Time PCR of Ho1 mRNA levels in spleen and liver of mouse models with enhanced erythropoiesis. (A) Ho1 mRNA levels in the spleen of Hpx, PHZ-treated, hypoxic, and iron deficient mice. Analysis by Students' T-test showed significant effects of Ho1 only in iron-deficient spleen tissues, (P < 0.01) over the control samples. (B) Ho1 mRNA expression in the liver of Hpx, PHZ-treated, hypoxic, and iron deficient mice. Analysis by Students' T-test showed significant effects of Ho-1 mRNA in Hpx (P < 0.05), and PHZ liver tissues, (P < 0.01) over the control samples. Data are mean ± s.e.m. of four observations in each group and are expressed as a ratio of 18S mRNA in arbitrary units.
Figure 4. Western blot analysis of Abcg2 protein and Ho1 levels in the spleen samples from Hpx, PHZ-treated, hypoxic and iron-deficient mice. (A) Abcg2 protein levels in mouse spleen samples that were homogenized and subjected to Western blotting as described under methods. (B) Abcg2 protein levels in the spleen tissues. Densitometries of the bands (upper) were quantified with Image J software and data were normalized with the levels of β-actin protein in the samples. (C) Ho1 protein levels in the spleen tissues from mice. Densitometric data are presented as the mean ± s.e.m. of four experiments in each group. *P < 0.05, **P < 0.001.
Abcg2 mRNA expression showed decreases in mouse model with enhanced erythropoiesis in kidney tissues (Figure 5A) but the response were not significantly different. Ho1 mRNA expression was significantly enhanced in kidney tissues from Hpx and iron-deficient mouse models (Figure 5B). Only modest increases in ho1 mRNA expression levels were observed in kidney tissues from PHZ-treated and hypoxic mouse models.
Figure 5. Quantitative Real-Time PCR of Abcg2 and Ho1 mRNA levels in the kidney tissue samples from mouse models with enhanced erythropoiesis. (A) Abcg2 mRNA levels in the kidney of Hpx, PHZ-treated, hypoxic, and iron-deficient mice. Data are mean ± s.e.m. of four observations in each group and are expressed as a ratio of 18S mRNA in arbitrary units. (B) Ho1 mRNA levels in the kidney of Hpx, PHZ-treated, hypoxic, and iron-deficient mice. Data are mean ± s.e.m. of four observations in each group and are expressed as a ratio of 18S mRNA in arbitrary units. Analysis by Students' T-test showed significant effects of HO-1 mRNA levels in Hpx (P < 0.01), and iron-deficient spleen tissues, (P < 0.01) over the control samples.
Abcg2, an efflux protein with broad substrate specificity, is characteristically localized and expressed in epithelial membranes of barrier tissues such as the intestine, placenta, liver and the brain. The regulation of cellular haem and porphyrin levels by abcg2 plays a pivotal physiological function in erythropoiesis and iron metabolism in mammals. Abcg2 mRNA expression coincides with enhanced stimulation of haemoglobin biosynthesis (Zhou et al., 2005), during differentiation between BFU-E and CFU-E. Globin transcription and iron uptake are enhanced and haem concentration is critical for the initiation of haemoglobin synthesis (Krishnamurthy and Schuetz, 2005). This fact might be the reason for the increase in abcg2 mRNA expression in the hemopoeitic precursor cells in mice that were administered with PHZ (Figure 1). As haemoglobinization increases with the progression of erythropoiesis, proerythrocytes presumably express low levels of ho1 and are highly sensitive to low levels of cytosolic haem. Abcg2 prevents porphyrin toxicity especially under hypoxic conditions, during the mid to the distal stages of erythropoiesis. Flvcr1 also effluxes excess haem during erythropoiesis and erythrophagocytosis (Keel et al., 2008; Chiabrando et al., 2012; Byon et al., 2013). Typical of promoters devoid of the TATA box, abcg2 promoter has multiple transcription sites (Lin et al., 2001). Zong et al. (2006) showed multiple transcription start sites from several leader exons in abcg2 gene. Moreover, these promoter regions are enriched with several transcription factors binding sites (Ishikawa et al., 2013). Furthermore, abcg2 promoter is regulated by tissue specific transcription factors such as GATA 2 (Minegishi et al., 1998). Consequently, abcg2 gene regulation is varied and multifarious in tissues and during diverse physiological processes.
Similarly, abcg2 mRNA expression (Figure 2) and protein levels (Figures 4A,B) were consistently up-regulated in the spleen of mice with enhanced erythropoiesis. Abcg2 and, more recently flvcr1 (Vinchi et al., 2014), have been reported to regulate haem levels in cells during erythropoiesis. Evidence from literature suggests a reciprocal pattern in flvcr vs. abcg2 expression as differentiation progresses (Keller et al., 2006). Flvcr null mice are non-viable and this confirms a lack of functional overlap with abcg2. Flvcr is presumed to efflux haem into circulation from macrophages (Keel et al., 2008) and possibly functions specifically during erythrophagocytosis. The latter report implied that a proportion of haem from phagocytosed, senescent red blood cells in macrophages is not degraded but effluxed into circulation by flvcr. This haem "cargo" could subsequently be bound by hemopexin for delivery to the liver. It has recently been shown that hemopexin is obligatory to the functional haem efflux capability of flvcr (Yang et al., 2010). Flvcr, in contrast to some other members of the major facilitator superfamily, is a unidirectional, vectorial transporter.
Similarly, splenic expression of abcg2 might also correlate with its role in detoxification processes. Haem, apart from being endogenously synthesized ubiquitously by erythroid and non-erythroid tissues, is also derived from haemolysis and haemorrhage (Reeder et al., 2002). While PHZ-treated mice are characterized by increased haemolysis and destruction of red blood cells, ho1 mRNA expression was significantly enhanced in the liver rather than in the spleen of these mice (Figures 3A,B). The reasons for variation in the basal levels of ho1 in the different mice models used in this study remain, however, both puzzling and unclear. The regulation of haem thus favors the depletion of unbound forms (Vinchi et al., 2008) to ameliorate toxicity in cellular and subcellular loci of visceral and peripheral tissues. It is well documented that ho1 expression is highly inducible in the presence of haem (Schwarzer et al., 1999; Desbuards et al., 2009). Consequently, intracellular and extracellular trafficking of haem is regulated to molecular channeling in organelles and ensures detoxification as necessary. Ho1 mRNA was generally enhanced in the kidney tissues from mice in the current study (Figure 5) although kidney abcg2 mRNA levels were not significantly different. The kidney is important in iron metabolism for the biogenesis and biodegradation of haem. The former is by a regulatory feedback loop of erythropoietin production and the latter by the glomerullar filtration of haem by megalin and cubilin receptors (Gburek et al., 2002, 2003). Consequently, Abcg2 might not be involved in the efflux of haem/porphyrin in the kidney as it does in erythroid progenitor cells. Perhaps megalin and cubilin function in concert and are complementary with ho1 to exude the resultant porphyrin breakdown products of haem: this remains an unanswered question.
In conclusion, abcg2, effluxes excess porphyrin metabolites and possibly excess haem during erythropoiesis particularly under hypoxic conditions. Egress and detoxification of excess porphyrin/haem by abcg2 and flvcr to maintain cellular homeostasis is presumably complemented by ho1 expression during the early stages of erythropoiesis. Consequently, abgc2 mRNA levels are stimulated during enhanced erythropoiesis in extramedullary haematopoietic splenic and liver tissues of Hpx, PHZ-treated, hypoxic, and iron-deficient mice. However, abcg2 expression is low in the kidney, and in tissues of this organ ho1 degradation of haem might predominate. Tissue-specific expression of haem influx/efflux proteins and haem catabolism by ho1 must be critically balanced to maintain optimal levels of haem for metabolic processes.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The study was supported by a Value in People (VIP) grant award from the Wellcome Trust.
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Keywords: Abcg2, bcrp, hypoxia, phenylhydrazine, iron
Citation: Latunde-Dada GO, Laftah AH, Masaratana P, McKie AT and Simpson RJ (2014) Expression of ABCG2 (BCRP) in mouse models with enhanced erythropoiesis. Front. Pharmacol. 5:135. doi: 10.3389/fphar.2014.00135
Received: 14 January 2014; Accepted: 19 May 2014;
Published online: 04 June 2014.
Edited by:Raffaella Gozzelino, Instituto Gulbenkian de Ciência, Portugal
Reviewed by:Andrei Adrian Tica, University of Medicine Craiova Romania, Romania
Emanuela Tolosano, University of Torino, Italy
Copyright © 2014 Latunde-Dada, Laftah, Masaratana, McKie and Simpson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Gladys O. Latunde-Dada, Diabetes and Nutritional Sciences Division, School of Medicine, King's College London, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NH, UK e-mail: firstname.lastname@example.com


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Throughout the year we write Case Studies about the projects we complete, click through to read more about each in detail. We're also very proud of the testimonials we receive, you can have a read of some of our favourites here.
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Disaster Designs and Religion Clothing moved their entire legacy computer estate to the cloud. Following rapid growth, they were continually hitting capacity, resulting in sporadic expenditure; the company were in need of a predictable, scalable model, which mtech.cloud provided.
October 2016
mtech.cloud, Nutanix, Avaya, IGEL, Citrix, VMWare
Sussex Plumbing Supplies, a long standing client of M-Tech, moved their entire ICT infrastructure to the cloud, providing unlimited flexibility and scalability, with unrivalled security, for the ever-expanding multi-branch Sussex-based merchant.
April 2016
mtech.cloud, Nutanix, Avaya, IGEL, Citrix, VMWare
Bourne Education Trust first started working with M-Tech in 2016. In the past year they've undergone further projects including Windows 10 and Microsoft upgrades, adding schools to the central domain and infrastructure upgrades.
March 2019 – Update
Nutanix, Palo Alto, Smoothwall, VMWare, Veeam, Connectivity, Multi-Academy Trust
The old school IT infrastructure had become too complicated and large parts of it were no longer supported, which was having a negative impact on teaching. M-Tech helped Milton Abbey move to new infrastructure, improving network reliability and performance.
December 2018
Microsoft Windows Server 2016, Windows 10, Microsoft MDT, Office 365, Active Directory & Group Policy, Citrix Virtual Apps and Desktops 7, Citrix Gateway
Bethany School needed to upgrade their wireless to support the growing demands of the 500 students staff across the large campus. The school were also embarking on bringing digital technology into the classroom, requiring a more stable, wider connection with higher bandwidth capacity.
January 2017
Aerohive, Enterprise Wireless
Richard Taunton Sixth Form College were in need of an upgraded server solution. M-Tech won the tender and were able to implement the project with minimal downtime. Richard Taunton opted for hyperconvergence from DataCore, using repurposed equipment where possible.
December 2016
DataCore, VMWare, Veeam, Hyperconvergence
The existing wireless and network infrastructure at Falkner House was in need of an upgrade to allow the school to further develop their innovative use of IT in the classroom. Combined with a move to the cloud, this enabled state of the art data protection for the school.
December 2016
Aerohive, Enterprise Wireless, RES, mtech.cloud
Eastbourne College are leading the field in adopting hyperconverged technology in an education setting in the UK. Nutanix affords the school high availability, resilience and performance on readily scalable 'invisible infrastructure'.
July 2016
Nutanix, Hyperconvergence
Alexandra Park recognised the need to refresh their technology and review the management tools they used to assist in the day to day running of the network.
May 2016
DataCore, RES, VMWare, Veeam
The implementation of a fully managed enterprise-grade wireless network has enhanced the availability around the school, as well as provided the additional benefits of accountability and management.
March 2016
Aerohive, Enterprise Wireless
The time spent on network control of the desktop has reduced by as much as 50 percent, which has allowed the IT team to refocus on the future development of the school's systems.
February 2016
RES, Windows Desktop Management
Uplands Community College regenerates their Virtual Desktop Infrastructure using the combined IOPs of DataCore's SANsymphony-V10 with Flash PCIe Storage.
February 2016
M-Tech Systems offer IT services, IT solutions and IT support to SMB, enterprise and education clients of all shapes and sizes.
We operate all across the UK, with special focus on East Sussex, West Sussex, Kent, Surrey, Hampshire, Essex and London.
We can work in any way that suits you, either on a per project basis, alongside your existing team to bring additional expertise, or as a fully outsourced IT department, providing a service desk.


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Mr Jordan
1. Wood cars with wooden wheels.
2. Parking garages underground so as not to take up space.
3. Bike team jerseys that say IDEA.
4. Hoodies at have a red light on the left shoulder and a green one on the right (like a boat)
5. All City super soaker fight (open to the public)


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September 2021
17th Biennial Worldwide Congress Unified International Technical Conference on Refractories (UNITECR 2021)
The Unified International Technical Conference on Refractories (UNITECR) is a biennial international conference that contributes to the progress and exchange of industrial knowledge and technologies concerning refractories. Refractory organizations from around the world will collaborate on the biennial conference planned for Sept. 14-17, 2021, in Chicago, Ill. Meeting History The organization of UNITECR was established in 1987 in Tokyo by the founding members. The official governing body is the UNITECR International Executive Board (IEB), composed of representatives of the Founding…Find out more »


text: Larger hand entwines with the smaller and softer, I lie still and listen to the beauty that is your breath. I sink into the warmth and innocence of your sleepy body, and am lulled into that blissful gap, neither awake nor asleep. I listen quietly for that which dwells in the space between. I reach out, to grab hold, before the words drift into the gap of another.


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Hello and welcome to EmpowHer,
Postmenopausal bleeding can be caused by a number of things. Most of the common causes are not very serious. Below I have pasted a link to an article that lists all the possible causes.
I hope that helps.
Have had two episodes of PMB and just had transvag u/s. Test was done on Wednesday, results received by doc's office on Friday last week. Unfortunately, doc had already gone home when I called to find out results. Nurse was unable to tell me results but assured me that it wasn't cancer. Also said there would be more tests. What could it be? I will definitely be calling doc's office again on Monday but in the meantime I'm worrying myself silly. Can anyone provide any reassuring information?
Add a Comment1 Comments
Hello and welcome to EmpowHer,
This Comment
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I tried partstown already but the model I need it is not listed..
I tried partstown already but the model I need it is not listed..
FWIW: I've repaired food equipment for twenty years. I often go to manufacturer's websites for manuals. I just did that for BKI and, I must say, their availability of literature (or ease of access to it) is among the worst.
Anyway, I believe your model (CPE series) is pre-2012 and was made in Denmark by a company named HOUNO.
Beginning in 2012, BKI teamed up with Giorik - an Italian company - who makes the BKI-badged combi-ovens up to today.
I happened to have a couple of manuals on those ovens which I've uploaded for you HERE:
BKI Combi King CPE-KPE Service & Installation Manual
HOUNÖ BPE-CPE-KPE Service & Installation Manual
The first manual was published in 2008 - obviously by BKI. The second one is unknown but I'm guessing is far more recent since it refers to the Middleby Corporation. Middleby is a direct competitor to the Standex Group. Standex owns BKI.
Don't get too wrapped around that. Just my nod in acknowledging the confusing world of the business world's constant changing of hands.


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leaks The Galaxy A90 comes with a pop-up and recyclable camera design as well
The latest release of the Samsung Galaxy A90, the latest release, confirmed that the next release of the Samsung Galaxy A90 is launching the camera's pop-up and recyclable design.
|Samsung Galaxy A90
Samsung seems to be trying to experiment with the various designs of the front camera in its mid-editions this year. The latest leaks revealed Samsung's plans to launch the Galaxy A90 with a mechanical camera design and a camera-supporting engine.
According to the latest leaks, the Galaxy A90 is also designed to emulate the distinctive camera of the Oppo Find X and the Oppo N1, which is designed to support both a master camera and a Sylphy camera.
The previous leaks confirmed that Samsung will present the slider or camera design in one of its medium versions this year, but the new details about the recyclable design of the camera is certainly an unconventional design in the Samsung releases, but leaks may reveal images that confirm this design and mechanism of the camera. The next Galaxy A90.


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Tom Wright invites you on an Advent journey with the apostles Peter, Paul and John, linking his reflections to the Sunday readings for Year B in the Revised Common Lectionary.
Join Tom Wright on a journey with the Apostles, exploring the New Testament themes of thankfulness, patience, humility and joy.
Within each of these themes, Wright offers a week of daily readings and meditations, beginning with the Sunday reading in the Revised Common Lectionary and ending with stimulating questions for personal reflection or group discussion.
Drawing on key passages in his popular For Everyone series of commentaries, these sparkling reflections take you on a journey of spiritual enlightenment, guiding you towards the wonder and joy of Christmas.
Select how many individual items you would like:


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The Repeat Dress
When it comes to the bold, statement garments in my wardrobe, I'm usually apprehensive to wear them more than once in the same place or around the same people. Though it sounds frivolous, it's true! The louder the gown, the more memorable it is.
There is one dress in my wardrobe, however, that never gets old. I bought it at TopShop in October along with some embroidered sandal pumps. The tonal contrast of warm and cool actually works well here (eight years of color theory classes will make you think about these things)! I wore the outfit out to a nice date on Friday night and to my step-sister's wedding on Saturday (though I was advised to wear a cardigan, as backless and spaghetti straps don't always float well at a family gathering)...
I wore it again this February for a pre-birthday, birthday dinner date, and received just as many compliments! Lace-up heels, embroidery, and backless dresses are a perennial showstopper, no matter how many times you wear them.
Photos by Forrest Culotta


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Berkshire Cricket Foundation and the England and Wales Cricket Board are committed to providing an environment that is safe, welcoming and friendly for children and for all people to enjoy the game of cricket.
The England and Wales Cricket Board's safeguarding policy, Safe Hands, is set out in detail on the ECB website.
Click here for invaluable Club Welfare resources including:
Key Definitions and Concepts
Safeguarding and a Club's Duty of Care
Safe Hands Safeguarding Kit Bag
Putting things in place (background work, policies and paperwork)
Having the right people in place (recruitment and guidance)
What to do if you have concerns
Cricket specific safeguarding guidance
Safeguarding in cricket is based upon providing an enjoyable environment that meets the needs and requirements of children.
The ECB Safeguarding Adults Policy can also be downloaded by clicking here.
The County Welfare Officer in Berkshire is David Brown, who can be contacted via email, firstname.lastname@example.com and / or by telephone .
Safeguarding Courses for Club Welfare Officers
Club Welfare Officers (CWOs) need to complete the Safe Hands and Safeguarding and Protecting Children (SPC) workshops every 3 years.
We have two courses organised
- Monday 18th March at Bradfield CC, 6pm - 9.30pm - book on here
- Wednesday 27th March at Boyne Hill CC, 6pm-9.30pm - book on here
DBS (Disclosure and Barring Service) Checks and ECB Vetting
The following roles in cricket that require a DBS (formerly CRB) check vetted by ECB are:
Welfare Officer (Club, League, County)
Volunteer Co-Ordinator
Coach (Volunteer Or Paid, Assistant Or Lead)
Colt/Junior Manager
Age Group Manager
Open-Age Team Captain
Junior Supervisor
First Aider / Physiotherapist / Medical Support
The ECB guidance on the roles in cricket that require a vetting check are available here.
DBS checks in Cricket are now via the online platform, please contact your Club Welfare Officer to initiate this process.
County Welfare Officer
Do you hold a valid safeguarding qualification from another sport or profession?
Such a qualification may mean you do not need to complete a further Safeguarding and Protecting Children course. Contact the County Welfare Officer (details above).
Safeguarding Courses for Coaches
Coaches working with players under 18 (as at 31 August) must obtain or renew their safeguarding qualification every three years.
To renew Safeguarding Young Cricketers (SYC) online and for free, sign up here and ECB will send you confirmation in order to take the course.
Then log in at the ECB e-learning website - click here to recover your username or password.
After following these steps email ECB e-learning in the event of any query.
As an alternative coaches may attend face to face Safeguarding and Protecting Children (SPC) workshops which can be booked via the UK Coaching website (usual cost around £30).
Coaches working with players under 18 (as at 31 August) must obtain or renew insurance cover, an ECB Disclosure and Barring Service (DBS) and first aid certificates every three years.
For further information please visit the courses for coaches page of our website.
More Info
Read the ECB's statement on Safeguarding https://www.ecb.co.uk/news/226784.


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Editor's Note: Today in New York City I dressed out of Riley's closet. We got so much done yesterday that we had time to go to the D&D building today and I did not want to wear shorts so I have one a la Riley a gap stripped skirt, Banana Republic top, and my Vince Camuto shoes.
Michelle and I accomplished so much yesterday that today we got to start our morning out picking up the Samuel & Sons trim for Riley's drum chandeliers and go into the D&D building next door. I had fun showing Michelle some of my favorite showrooms in the building! Where are vendors are grouped together in showrooms in Dallas they are all individual in the D&D building. Every showroom has more feast for the eyes and it is a beautiful experience! Here we are traveling to the D&D building and visiting some of my favorite showrooms!
Union Square farmers market which is so fabulous with local produce and flowers! On our way to the subway.
First stop Samuel & Sons to pick up Riley's trim. This window display was so precious!
A closer view of the precious dolls in the window!
This store is incredible with all these beautiful trims ready to cut and go!
The walls are a full of incredible trim. My favorite trim resource!
Here we go into the famous D&D building!
First stop Brunschwig & Fils---such an elegant entrance!
The showroom is stunning!
Next up Pierre Frey!
This incredible wallpaper was in the entry!
I did not know that they did custom carpets and rugs! I will be sure to get on that in the near future!
Our fabric that we are using on our Palm Springs job in one of the guest rooms. We have titled that room the Cactus Room!
Here were are in Vaughan showroom. I love this pink print! Speaks one of my clients names!
A wall of Vaughan's beautiful fabrics!
On to Lee Jofa! This is a new room with all of Kelly Wearstler's products. So beautiful!
This incredible wallpaper! It covers 9 feet of a wall and was in the entry! So lovely!
Some of the Lily Pulitzer fabrics! So happy and fun!
Some more new additions from Aerin Lauder! I love going to the wings with the new fabrics!
A stunning vignette in the middle of the showroom!
On to Kravet! I am seated on my favorite Allegro sofa. It is a true lounge lizard sofa!
About to leave to carry on with our day. These beautiful vignettes was in the middle of the entry hall of the D&D building.
This Hermes one was so special!
Great quotes!
Steel Magnolia Tip #30: A steel magnolia has the power within her to solve all sorts of problems. She just has to recognize and release the power.
We were so tired from our install that we forgot to take a picture of our dinner. We had take-out thai food in Riley and Flo's new apartment! We had fun drinking wine and enjoying the fruits of our labors all day. The apartment is so darling!
Great song from my past! I never grow tired of it.


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I made this a few weeks ago but got swamped and haven't had a chance to update and put it up so I'm not positive the story behind it. Oh well, I'm sure it wasn't that interesting anyway.
I made chicken for B and made shrimp for myself. The chicken I added water to occassionally to make sure that the adobo mixture (and chicken) stayed moist.
Adobo Chipotle Chicken (and Shrimp) Tacos
1/3 cup apple cider vinegar
1 lime
4 chipotle peppers – canned in adobo sauce
1 cup onion, chopped
5 cloves garlic
4 tsp cumin
1 ½ tsp ground black pepper
1 ½ tsp salt
1/8 tsp allspice
2 tbsp vegetable oil
2 boneless skinless chicken breasts, thawed
10 shrimp, thawed
2 1/3 cup vegetable broth
3 bay leaves
1 oz gin
1. Combine the lime juice, vinegar, onions, chipotle peppers, garlic, cumin, allspice, black pepper, and salt in a food processor and blend until smooth and creamy.
2. Remove any excess fat from the thawed chicken.
3. In a large dutch oven, heat 2 tbsp of oil and cook each side of the meat pieces until lightly browned. (heat 2 tbsp of oil in a sauce pan for shrimp)
4. Pour the blended sauce over the meat (or shrimp) and add in the vegetable broth, bay leaves and gin.
5. Cover and cook on medium heat for about 2 hours, turning every twenty minutes or so until the meat can easily be pulled apart. (For shrimp simmer for about 10-15 minutes)
6. Serve with lettuce, shredded cheese, taco shells, and your favorite taco fixins!


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Gwenaëlle Aubry • Personne
Translators must make do.
This French novel was published in English in 2012 as No One, which is both accurate and entirely insufficient. Its original title, and the title I will use in this review, is Personne.
As a noun, French personne is equivalent to English person. As an indefinite pronoun, however, personne means no one. The word's root is Latin persona, which means a mask used in theatre. All three of these resonances are essential to understanding the novel, and there is no English word that captures them all. Reading the last sentence of the novel you understand why they went with No One rather than Person or Persona, but any is insufficient. So for this review, I'm going to use Personne.
The translation otherwise is very readable, as if it had been written in English originally.
Gwenaëlle Aubry, Personne [No One], trans. Trista Selous. 2009 autofiction about the author's relationship with her father, who struggled with bipolar disorder. Recommended.
Personne is an autofiction based on the journals of Gwenaëlle Aubry's father. François-Xavier Aubry was a lawyer who suffered from bipolar disorder, and who, if this book is anything to judge by, did not treat his family the best. He kept a detailed journal, which he named The Melancholic Black Sheep. He wrote a note on the front: "To be novelized." And a subtitle that he crossed out: A Disturbing Spectre.
Aubry blends passages from this journal, from her own memories, and from books and movies in a circuitous yet systematic exploration of her father. She writes poetically and sometimes philosophically.
A word about genre. Autofiction, as explained in Rick Moody's introduction to Personne, is a "term for the stylized hybridization of fiction and autobiography as applied in contemporary literature." How much of Personne is real and how much is fiction, I don't know. What is very clear from the book's form, though, is that Aubry has shaped the material to fit a scheme.
Personne is remarkably moving despite how strongly Aubry foregrounds the its metafictional elements. The short book has twenty-six chapters, one for each letter. This form makes translation impossible on occasion, such as Chapter E for "Enfant [child]." She calls it "this meaningless order in which I've tried to hold his disorder and mine, to smooth out our memories…" These chapters are fragmentary views with a subject determined by the letter. Some of the letters work better than others, but the structure is not so imposing as to draw away from the emotional heft of the whole.
The chapters are mostly short. Events are told out of order, with Aubry circling back repeatedly to particular events or images. She's searching for who her father really was, while recognizing that both his personality and the disorder that so strongly determined her father's behaviour meant that he was a multiple person or a wearer of masks without a set person below them.
The triple resonance of the word personne is felt throughout: persona, person, and no one. A persona was a mask worn by an actor; Aubry's father wears many masks, tries to be many things: "the Prodigal Son and the Spurned Lover, the Clown and the Pirate, the Cop and the Robber, the Monk and the Rake, the Bourgeois and the Tramp, the Sage and the Madman." Beyond any mental disorder, this was his personality. Aubry is searching for the person who her father was below those masks, but the evidence—journal, memories, intertexts—is inconclusive, as it will always be. She finds no one.
The chapters may be short, but Aubry loves very long sentences. Here's a taste of the novel's beauty and its investigative style.
At the end of his life my father didn't want to be anything. By that I mean he wanted just to be, to take off his masks, cast off his rags, abandon the roles, the characters that throughout his life he had expended so much effort in playing, shed the qualities he had put on one by one, seeking the one that would define him, give him form and content, that would at last turn him into his own statue, a silhouette of marble with clean contours, sharp lines, a person, a complete man, a man of quality, one of those who stride through the streets in the broad light of day without ever wondering why they are themselves and not the shadow that clings to their footsteps…
And that is only half the sentence!
There are numerous intertexts throughout the autofiction, which help Aubry explore her father's identity. Chapter A is for Artaud, and the novel begins by talking about Artaud, who, like Aubry's father, was locked up in an asylum and who described, "worse than pain, than eternal hell, the exploding of his real self." Artaud is also briefly revisited in the beautiful Chapter T for Traitor, which is a sort of eulogy or prayer to her father's memory:
to the memory that returns, sharp and clear, one sleepless night, of his smell, his warmth, his arms around me when we had to part, those moments when he was, yes, a rampart, strength—when, like anyone else, I had a father
These intertexts also include such material as her father's lookalikes, Jean-Pierre Léaud and Dustin Hoffman, and her father's interests, like James Bond. Her father wanted to be James Bond, because "becoming James Bond meant disappearing, slipping out of sight, going back to the rift within himself, hunkering down in its shelter." The intertexts provide Aubry a type of objective viewpoint on the painful image of her father.
Aubry's memory is the final source of material. She compares what she remembers with what's written (or not written) in the journal. Her memories are often painful, but she tries to view her father generously.
I found it hard to recognize the elegant, settled, attentive man who came over to visit me in England, called me all over the world and took an interest in my studies and plans, who behaved like a father, as my own. I didn't believe in miracles. But sometimes, in certain silences, moments of despondency, I saw the familiar abyss. We didn't talk about it. And I felt as though, having tried so hard to forget, my memory was now going mad. He didn't want to remember, didn't want to know. Or rather, knowledge fostered his great desire to forget.
Personne is a tremendously moving book, "this portrait from twenty-six angles with an absent centre, this portrait in twenty-six others with a self that eludes it." Aubry's great achievement is to find the emotional rawness in the balance between three sources of material—journal, memories, intertexts—an arbitrary metafictional structure, and her own deeply considered interpretations.


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A weekly stand up showcase at the swankiest crepe restaurant in Burlington. Featuring local talent, as well as up and coming comics and established names from all over New England. Oh and it's free!
Back to All Events
Earlier Event: January 14Comedy @ Sweet Melissa's
Later Event: January 21Socks and Sandals


text: Rob McHale is a North Carolina-based folk-Americana artist whose songs can take you through a small town, down a dirt road or on an historical journey – and bring you back home again. What we have here is ten beautifully realised songs, arranged, produced and performed with maturity and a style that is very appealing. The production by Chris Rosser and the band ensemble of 11 musicians deliver on all fronts. I can hear early Jackson Browne in the gorgeous Back Home with harmony vocals to lift you away from your reality and the songs Mother's Love, Fields and Wishing Well add to the feel of authentic folk music. Real and mature, reflective and sincere, this is music for the spirit and a really excellent release.


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Software development
I can provide services in the field of software development based on the latest technologies in Java and various Java-based frameworks.
I'm experienced in requirement analysis, design, development and testing, implementation and support in:
- Core Java
- Adobe CQ5/AEM
- Spring Framework
Additionally my company has access to group of good developers for implementing larger projects.
We can help you with:
- Requirements gathering
- Solution design
- PCB design
- uC software development
- Integration with existing solutions
- Management software design and implementation
For more information see list of projects I've been working on.


text:
When: Monday, December 14, 2015
Reception: 5:00pm - 8:00pm
Gaylord National Resort & Convention Center
201 Waterfront Street
National Harbor, MD 20745
Shuttle Bus Service:
A shuttle bus will be available to take attendees to the Gaylord Hotel from the King Street Metro. Please look for a signage that says "AMPs Holiday Party" to locate the shuttle bus at the King Street metro station. Below are the shuttle bus departure times:
***Note pick-up times may vary a few minutes due to traffic***
From King Street Metro to Gaylord Hotel:
1st shuttle: 4:15 p.m.
2nd shuttle: 5:30 p.m.
3rd (and final) shuttle: 6:15 p.m.
From Gaylord Hotel to King Street Metro:
1st shuttle: 7:15 p.m.
2nd (and final) shuttle: 8:00 p.m.
Parking: Complimentary
PLANNERS: $30 in advance and $40 onsite.
SUPPLIERS: $55 in advance and $65 onsite.
SUPPLIER PACKAGE: $130 (includes 1 supplier ticket and 3 planner tickets- Once purchased, email [email protected] to obtain your 3 planner codes)
Advance Registration closes on Friday, December 11th at 11:59pm
Check us out on Facebook!
Thank you to our Holiday Party Sponsors!
Thank you to our Premiere Sponsors!
Calendar powered by The Events Calendar


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P. D. James, Devices and Desires
This twenty-year-old novel proves once again that P. D. James is truly a master of the mystery genre. In this installment of the Adam Dalgliesh mystery series, her protagonist finds himself on England's sparsely populated headlands to attend to matters of his deceased aunt's estate. Meanwhile England's latest serial killer is on the loose. And his latest victim is an employee at the near-by nuclear power plant that dominates the headland. Though Dalgliesh is off-duty while out in the country, his proximity to events, and his discovery of what seems like the latest victim while walking along the beach involves him in the mysterious events.
This book, like all of James's mysteries, is filled with well-developed characters that give verisimilitude to her stories, that give real humanity to the victims, to those touched by the killings, and even to the suspects. This serves both to give depth to the narrative and to heighten the tension of the mystery, as it makes suspects more interesting but also keeps you guessing as to who the real perpetrator may be. Devices and Desires also contains some great dialogue that probes deeper issues, such as the detective's relationship to death, or the possible continuing relevance of the category of sin, or the possibility of justice in a world full of twisted devices and desires that enmesh our lives. This mystery does not disappoint. It is well written, thoughtfull, and entertaining, and comes to a satisfying conclusion. I highly recommend it.


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WTO decision in never ending saga
The World Trade Organization (WTO) said today that it had upheld for the most part a ruling that Boeing received billions of dollars of subsidies to compete with Europe's Airbus, as both sides once again claimed victory in the long-running trade row.
The subsidies included at least $2.6 billion in assistance from US space agency NASA, which the WTO's appellate body agreed had allowed Boeing to launch the 787, causing what it described as "serious prejudice" to its European rival. The ruling is the latest step in a seven-year dispute involving mutual claims of aid for the world's dominant OEMs and could theoretically lead to retaliation on both sides once the Geneva trade body's procedures are exhausted. The European Commission welcomed the WTO ruling, saying it confirmed billions of dollars in US subsidies to Boeing were illegal under WTO rules. "Today's ruling vindicates the EU's long-held claims that Boeing has received massive US government hand-outs in the past and continues to do so today", said EU Trade Commissioner Karel De Gucht. The response came as the US took the opposite view, saying the WTO decision confirmed that Europe's unfair trade subsidies to aircraft maker Airbus had dwarfed US aid to Boeing.
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When five-hospital health system HIMA San Pablo sent an imaging contingent from its home base in Puerto Rico to RSNA in Chicago back in 2003, no one in the group could have foreseen how fortuitous their trip to the Windy City would prove almost a decade and a half later.
If you're going to operate a hospital outpatient imaging facility so distant to the nearest city that your location overlooks a sprawling cow pasture, you need to give patients a reason to make the drive. OGH Imaging in Grand Coteau, LA.—population 939—has been doing just that ever since opening its doors in 2005.
Six years ago, the non-invasive cardiovascular lab (NICL) at 540-bed St. Dominic Hospital in Jackson, Mississippi, faced something of an uncertain future. The rules for maintaining accreditation, set by intersocietal accreditation commissions, require that all vascular exams get interpreted within 48 hours of image acquisition. For cardiac exams, the read must wait no longer than 24 hours.
2018 has already been a year full of breakthroughs in artificial intelligence (AI) and machine learning, and those technologies are sure to receive a lot of attention March 5-9 at HIMSS 2018 in Las Vegas.
When it comes to ramping up on imaging clinical decision support, now is the time to assess your choices, set expectations, map out a plan and get the wheels of implementation turning. Imaging leaders need to play a key role in implementing CDS, educating ordering physicians and ensuring program success.
West Feliciana Hospital (WFH) has been serving patients in the small town of St. Francisville, Louisiana, since 1970, but its imaging capabilities were limited for a long time. As a result, the hospital gained a bit of a reputation among referring physicians in the area—when in doubt, they would just avoid WFH altogether and send patients more than 30 miles away to Baton Rouge.
Artificial intelligence (AI) was the center of attention at RSNA 2017 in Chicago, and FUJIFILM Medical Systems U.S.A., Inc. got in on the fun by unveiling its new AI development initiative from its booth at McCormick Place. Bill Lacy, FUJIFILM Medical Systems U.S.A., Inc. vice president of medical informatics, spoke to us after the show about both the history behind the initiative and its bright future.
If you put a leading-edge 3D visualization platform in the hands of a fearlessly tech-forward radiologist, don't be surprised if some real innovation emerges. That's one lesson to be drawn from a recent cross-subspecialty adaptation of a Fujifilm Synapse® 3D component called Sector MPR.
Hartford HealthCare is Connecticut's most comprehensive healthcare network. Over the last several years, this community and academic health system has grown significantly through its strategic affiliations with hospitals and a variety of providers.
After previously saying she had secured a commitment to pass the legislation by the end of 2017, Sen. Susan Collins, R-Maine, said bills to fund the Affordable Care Act (ACA)'s cost-sharing reduction subsidies and provide $5 billion in reinsurance will be left out of a year-end spending package.
To the surprise of absolutely no one, artificial intelligence (AI) and deep learning technologies were the talk of RSNA 2017 in Chicago. More than 70 sessions were devoted to AI, and you couldn't walk ten feet without reading or hearing the words "machine learning" or "algorithm."
Heading into the economically and politically turbulent year that 2018 is sure to be, radiology practices need all the navigational help they can get if they want to not only survive, but thrive in these uncertain times. In-depth assists are available, for free, from vRad for those dealing with staffing shortages, data security concerns, final-read demands and overall practice-performance issues. Here are descriptions and links to online resources that will help practice leaders navigate the course to becoming a high performance radiology practice.


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Video: Amazing CCTV footage shows wild elephant cleaning up rubbish
Footage showing a wild elephant picking up rubbish with his trunk and putting it in the bin has gone viral.
In the clip, the elephant clearly takes the cleanliness of his surroundings very seriously as he determinedly sets out to make sure the ground is litter-free.
The gentle giant is quite obviously setting an example for all of us, as he starts picking up bottles and other pieces of rubbish with his trunk and pops them straight into the bin.
He takes his time cleaning up, never missing a beat as he uses his trunk and one of his legs to pick up each piece carefully – even when he drops some rubbish, he picks it back up straight away.
The clip is only a minute long, but it is quite a sight to behold and the elephant is seen quietly walking away, one he is certain he gets his job done.
Online Editors


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Our Garden
What would we be if it weren't for our beloved Garden. Here at CB it is as Farm to Table as it gets. That Dreamsicle smoothie? Bananas from the trees right here on-site. That Kavocado salad? Kale greens grown mere feet away from our little orange food truck.
For us the garden defines us. We want to give you the whole shabang! The experience! When you sit down and look over the ocean, it is almost over shadowed by the beauty of our little farm. The garden allows us to give you a unique experience every time you come to see us. From the beautiful morning rays lighting up the dew drenched produce during coffee talk, to the mid afternoon, post surf smoothie sesh seeing west swell pass Tres Palmas, all the way to the serene Juicy hour sunset; dollar off for happy hour, the garden view has yet to disappoint.
The cool thing about it is, the party never ends. Because of the Puerto Rican Harvest, the action never ceases at the Garden. Year-round we are growing for you to enjoy. We're one of the only places in Puerto Rico who does it, so check in with us here to see what's up. We'll keep you posted on what's grwoing and when, so when that time comes to get pickin', you'll know when to come eatin'.


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Meet this cute brunette whore who is taking on two guys in this extreme facefucking video on FaicalAbuse.com. Watch them ram her mouth and throat with their cocks that goes all the way down to the balls. She has a meltdown but she managed to keep it together and ass and pussy is then double penetrated before her face gets covered in a creamy load of jizz.
This dumb whore is Catlynn and she considers herself as a "kinkster". She's so full of shit that she can't even handle the rough throat fucking that Pauly and Jay D was giving her. They pumped their cocks down her throat, slamming it like a tight pussy, making her gag hard. Her pussy gets ripped open before taking a creamy load of cum all over her face.
Watch Catlynn's Exclusive Facial Abuse Video in the Members Area
Throat Fucking Kat Monroe
This fresh faced whore is Kat Monroe who is trying to make a name for herself. She's not afraid to inhale cocks. She came to Face Fucking ready to be destroyed and the guys obliged. Harker and Jay D stuffed her throat with their hard cocks. Then the guys took turns slamming their cocks into her tight little cunt. Once they were done destroying her, she gets her face covered in a huge load of jizz.
See the Complete Kat Monroe's Video on FaceFucking.com
Hardcore Face Fucking Trish Kiss
This 19 year old mom-to-be is Trish Kiss who gets dicked down by a pair of magnificent mammoth meat sticks. She gets brutally face fucked so hard that tears were running down her face. Once she got through the face fucking, her pregnant teen pussy was punished and marauded by huge cocks, then her face gets covered with two massive loads of cum.
See the Complete Trish Kiss Video on FaceFucking.com
This bright-eyed and bushy-tailed is a 21 year old whore Keegan Kade who never imagined to be treated the way she did on FaceFucking.com. She is motivated by money becasue she doesn't have any, she almost quit but she powered through the rough throat fucking. The guys shoved their cocks down her throat for a brutal face fucking. That's when she got emotional as the guys were being mean to her. Then her tight pussy got slammed nice and hard before she gets her face all drenched in spit and cum.


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My husband of seven years is a serial cheater who\'s left me and our young son about 300 times. We\'re separated now, and he\'s engaged to his latest affair partner, but that didn\'t stop him from trying to sleep with my happily married friend or e-mailing me and begging me to take him back. Last week, I ran into his fiancee and showed her the e-mails. Needless to say, I broke them up. He says he\'s laughing at both of us now, but I think we had the last laugh, don\'t you?
— The Wife
I think your son\'s therapist will have the last laugh — probably while looking out over the yachts and vacation homes your son\'s bought her.
I also think your letter should remind the "family values" types in a panic to stop gay people from marrying and having kids to be a little more worried about all the straight people doing it. After all, for a gay couple, starting a family takes lawyers, adoption agencies and Guatemalan orphanages; it isn\'t like "Jeff" is going to wake up one morning with a shy smile on his face, turn to "Steve" and announce, "Guess who\'s pregnant!"
In my fantasy, the only people, gay or straight, who\'d be allowed to raise children are those who are almost too terrified, lest their progeny be best known as the fifth grade\'s top crack dealer. In reality, while you need a license to fish or cut hair, having kids takes only working ovaries and a sperm supplier; if not a willing one, one too drunk to parse whether "the pill" the woman\'s on is the birth control variety — or Advil.
There was a time to wonder whether you were enough of a grownup to be a parent — and it was long before Junior\'s 15th or 20th trimester. But first things first: wondering whether you were grown up enough to get married — ideally, prior to tying the knot with the kind of guy who not only has extramarital affairs, but extra-affair affairs, and probably needs flash cards to keep all the names straight. Still, even after you tied the knot, you could\'ve admitted you\'d made a mistake. Instead, you made a baby. Wheee! Cootchie-cootchie-crimewave!
What were you planning on doing, crossing your fingers and hoping your kid wouldn\'t grow up to pistol-whip and carjack Granny? Maybe he can\'t have two good parents, but if he\'s going to have some semblance of one, you\'ll have to bow out of the marital version of "Last Comic Standing." To do that, people will tell you you\'ll have to forgive the floozy magnet. They\'re wrong. Forgiving means excusing somebody\'s behavior. His behavior isn\'t excusable. What you need to do is accept reality: Nothing you say or do will make a devoted husband and father out of "Who\'s yer daddy."
You two owe your kid a happy childhood. Daddy needs to decide how involved he\'ll be in his son\'s life. Consistency is key. Refrain from badmouthing him, even silently, by sneering or eye-rolling, which your kid is bound to pick up on. As for healthy adult role models, it\'s clearly going to take a village. Join forces with healthy parents and families, and hang out with them regularly with your kid. Pick up two copies of The Good Divorce, by Dr. Constance Ahrons, so you and his dad can learn to parent as "cooperative colleagues" instead of vengeful brats. Hilarious as using his affairs as your personal whoopee cushion must be, you\'d better stop laughing and get to work burying the hatchet, or some shrink is going to spend a lot of years trying to pull it out of your kid.
Heinie happy people
I\'ve been seeing a 25-year-old woman for a short time. She isn\'t overweight by any means, but she definitely has a booty. I really like that on a woman, but she\'s self-conscious about it, and always covers it up in baggy clothes because her ex used to call her "wide ride" and other names. What can I do to help?
— Bringing Up The Rear
There\'s never been a better time to have a big dumpster. For an increasing number of women, butt implants are the new boob job. For a less invasive boost, there\'s underwear with built-in butt falsies. Unfortunately, nobody\'s come up with a prosthesis for flat, saggy self-worth — which is typically what makes a woman stick with a guy who calls her mean names. A girlfriend who\'s lacking on the inside is likely to make any relationship misery on some level, or at least kind of a drag. If that doesn\'t dissuade you, all you can do is keep showing and telling her how hot you find her — not despite but because of her apparent need to special-order panties from the company that made the cover for the Astrodome.


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Over the recent break, I went for a drive. And not a short drive either. I drove to Perth. It took 38 hours in the drivers seat to cover just over 3600km. Needless to say there was some time to kill.
I had prepared for this trip by downloading an audiobook called The Lean Start Up, by Eric Rimes. This book outlines the steps start-ups need to take to become more innovative, stop wasting peoples time and be more successful.
I recently promised myself to write more, and one way to do this is to share learnings from thing I read, work that I am involved in or observations of the industry. I hope to provide some gems of information, food for thought and thought for food. I allow myself no more than 20 minutes to write each post and no more than 10 minutes to edit and rephrase before I post.
So, follow along as I see how appropriate a Lean Start Up approach is for todays food industry!


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What is the IoT Edge?
Credit to Author: Cyril Perducat| Date: Thu, 08 Nov 2018 13:59:21 +0000
Each of us can point to a new technology that changed life as we knew it. The TV remote. The PC or Mac. Streaming music and video. In the recent… Read more »
The post What is the IoT Edge? appeared first on Schneider Electric Blog.Read more


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Staff at the Great Glove Thailand Co Ltd in Baan Manik reported the accident to Lt Suporn Muangkhai of Thalang Police at 11:50am.
At the scene, police and Kusoldharm Foundation and Srisoonthorn rescue workers found a yellow forklift flipped on its side on a mound of sawdust, with the body of Sujet Chuachokeamnuay lying nearby,
"From the preliminary investigation we concluded that Mr Sujet was driving the forklift as normal and lifting sawdust to move it. The vehicle overturned and fell on top of him," Lt Suporn said.
"A colleague who saw the accident used another forklift to help lift the toppled vehicle before checking the body of the deceased and notifying police," he said.


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The Salty Bee Bus
our on-site beer & wine garden. You can find Ms Barbie serving up cold drinks every weekend from the most unique bar window in the county.
Friday Nights
the Salty Bee offers live music & a food truck in the garden. Come on out for delicious food & drink while listening to some of the best local musicians around! The fun begins at 6 pm.
Saturday & Sunday
the Salty Bee is a welcome respite from the heat of the coastal summer sun. You'll find board games, yard games, cool shade and comfy seating as you sip a glass of chilled wine, an ice cold beer, soda & teas.
Office Parties, Birthday Parties, Reunions
the Salty Bee is available to host your next gathering. Let us help you plan the food, entertainment & beverage details to create a fantastic, fun outdoor event!


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Warning: Spoilers ahead for season two of Netflix's "Glow."
On the second season of Netflix's "Glow," we follow Sebastian "Bash" Howard on an unexpected journey of self-discovery linked to his sexuality. Though the show (and cast and creators) never explicitly state it, many of his scenes imply Bash may be realizing he's attracted to men.
"I think with all of our characters there are a lot of layers to explore," co-creator Carly Mensch said during a Television Critics Association (TCA) panel attended by INSIDER in Beverly Hills this summer. "We had a plan with Bash and with Chris [Lowell] from season one and we knew we wanted to tell this story."
Over the course of season two, Bash discovers that his longtime best friend and butler Florian is gay. This leads Bash to some emotional introspection that comes to a head when he hears the news that Florian has died from complications related to HIV/AIDS. Bash winds up proposing to Rhonda and they have an on-the-spot marriage ceremony.
"Bash doesn't know how to feel about all of it," actor Chris Lowell told TVLine. "He feels terrified and ashamed and scared and panicky, and I think that's what leads him to jump into this last-minute marriage to Rhonda."
Mensch and co-creator Liz Flahive were asked at the TCA event what this means for Bash's sexuality moving forward.
"We knew we wanted to go at the pace of that character and wanted to be emotionally honest and it's not something we wanted to do in a way that was rushed," Mensch said. "This is a character who is grappling with a lot and it's going to be a bumpy road. So we get to tell that story with Chris [Lowell] behind it, who is amazing."
As for the coming third season, we'll have to wait and see how Bash's marriage to Rhonda plays out, and whether Bash ever admits that he might be gay or bisexual.
"I feel like Rhonda is such a go with the flow, live in the moment kind of person, it be will be interesting to see what happens when she finds out about Bash's sexuality," Kate Nash, the actress behind Rhonda, told DigitalSpy.
"Glow" has been renewed by Netflix for a third season, which will likely take the Gorgeous Ladies of Wrestling to Las Vegas as the season two finale promised. The second season is streaming now on Netflix.
For more great stories, head to INSIDER's homepage.


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Sofas are the first of furniture that represent the taste and décor of the house. Give your guests an amazing first impression with the various designs of modern sofas available in our product line. Our products are sure to attract every eye.
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we offer a broad range of which meets your specific needs, including side boards, wall units, bookshelves, wardrobes, coffee tables, chairs, sofas, kitchens and bathroom cabinets.
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If you are looking for furnishing your home with quality and affordable furniture like living room furniture,Bedroom furniture,storage furniture,dining Room,Outdoor furniture then visit our Happyhome furniture store.
Number of links: 10


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Beyond Limits AI solutions go beyond conventional AI to unlock human potential and transform business. We bring a highly sophisticated approach to solving healthcare problems, combining conventional numerical AI techniques with advanced symbolic reasoning to provide human-like insights. We reduce risk, provide explainable solutions, and bring insights powered by human expertise and advanced AI.
Beyond Healthcare
For healthcare professionals, the problem isn't too little information. It's not too much information, either. The problem is getting the right information to make a better decision at the right time. The answer to providing more consistent care is to drive better insights from clinical data, scale expert knowledge, and distribute the expertise to the point of care. To assist physicians with today's difficult clinical decisions, we build clinical knowledge and experience into scalable expert decision-support systems. For patients with chronic or multiple conditions, the AI system can monitor the treatment regimen to reduce adverse medical errors that might occur due to complex polypharmacy or co-morbidities.
AI systems powered by Beyond Limits human-like reasoning provide clinical care options in a clear, evidence-based audit trail that can be queried for answers. Clinicians can't settle for "black box" solutions from conventional machine learning and deep learning approaches. There is too much at stake for their patients. Unlike "black box" approaches, Beyond Limits cognitive AI delivers clear explanations of its cognitive reasoning in transparent, evidence-based audit trails, including risks and uncertainties. We empower clinicians with the information needed to make the best care decisions for their patients.
The trend to more intensive patient monitoring is not going to stop. Unfortunately, most monitoring systems tend to be narrowly focused and rigid, and frequently produce alarm alerts without reference to overall patient health and treatment plans. False positives can lead to alarm fatigue, and in some cases, overlooked patient deterioration can lead to death.
The Cognitive AI Solution
Beyond Limits builds cognitive AI systems that interpret vast amounts of data from disparate sources to produce actionable information for patient care teams. Real-time monitoring, historical patient data, lab results, chart notes, evidence-based clinical guidelines, drug interactions, etc., are all combined by the system to better understand and personalize treatment suggestions.
In addition to diagnostic applications, Beyond Limits systems can prognosticate to proactively alert medical staff about patients who are deteriorating. The system explains its reasoning in clear, evidence-based audit trails and displays confidence values that express the probability of an alerted condition.
Only 5% of the US health system population accounts for more than 50% of costs, largely in hospitalization. Current systems for managing population health depend on manual processes – a sure prescription for inefficiency and excessive cost. A Beyond Limits AI system can assess longitudinal population health data or prospective risk factors so effective treatment options can be identified and promoted by PHM decision-makers. A cognitive system can also identify which patients are most likely to respond to care interventions. Symbolic AI from Beyond Limits can help PHM teams move from a 'one size fits all' for a given population to a more precise "one size fits one" approach. By optimizing care for individual patients and understanding risks, patterns, and trends for populations, outcomes can be improved while effectively managing costs.
Routine healthcare administration is hampered by outdated, manual, inefficient processes that lead to poor financial outcomes for providers. Beyond Limits cognitive AI can help organizations connect the dots while shifting from fee-for-service reimbursement to value based care.
Healthcare AI technology can reduce the friction, errors and cost in registration, scheduling, charge capture, health information management, and billing and collections. The goal is to reduce the number of denied insurance claims, speed explanation of benefits (EOB) reconciliation, improve the quality of information, streamline denial management, and automate processes.
To Avoid Repeat Denials, Break Through Silos
Redoing work erodes efficiency in any system, and in healthcare, claims denial is a big contributor to inefficiency. An AI system can manage denials by profiling claims by groups and associations according to their denial risk with traceability showing underlying variables that trigger denials. One obstacle to integration of RCM is that data from front-end and back-end systems reside in silos, with little to no ability for cross-analysis. Silos prevent comprehensive, systematic and data-driven methods to identify the specific reasons behind a pattern of claims denials. Our AI systems can employ human-like reasoning to eliminate this disconnect and can assess the denial risk of potential claims to intervene before a claim is submitted. Our systems can prevent problems, not solve them after the fact.


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Make an appointment0508 586 466
Save time & book online with instant confirmation at over 90 Lumino practices across NZ.
Book Online Now
309 Southampton St West, St Leonards, Hastings 4122
(06) 876 9983
The home of healthy smiles
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Important beginning steps to determine the health of your teeth, gums and mouth!
We offer a number of choices in types of fillings to ensure that you are satisfied and comfortable!
Take the first steps to good oral hygiene and healthy teeth and, gums!
Enjoy the several teeth whitening options that we have available!
Dental bridges bridge the gap created by one or more missing teeth.
A porcelain or gold cap that is used to rebuild and strengthen a weak or broken tooth!
The aim of root canal treatment is to save a tooth that has been badly damaged...
We have WINZ registered dentists located throughout New Zealand
A selection of practices offer private dental services for teens!
ACC may be able to help towards your treatment costs if you have a dental injury
Our paediatric dentists are dedicated to the oral health of children!