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Maternal alcohol consumption during pregnancy causes wide range of behavioral and structural deficits in children, commonly known as Fetal Alcohol Syndrome (FAS). Children with FAS may suffer behavioral deficits in the absence of obvious malformations. In rodents, the exposure to alcohol during gestation changes brain structures and weights of offspring. The mechanism of FAS is not completely understood. In the present study, an established rat (Long-Evans) model of FAS was used. The litter size and the weights of mothers, fetuses and placentas were examined on gestation days 18 or 20. On gestation day 18, the effects of chronic alcohol on the expression levels of integrin receptor subunits, phospholipase-Cγ and N-cadherin were examined in the fetal cerebral cortices. Presence of alcohol in the liquid-diet reduced the consumption and decreased weights of mothers and fetuses but increased the placental weights. Expression levels of β1 and α3 integrin subunits and phospholipase-Cγ2 were significantly altered in the fetal cerebral cortices of mothers on alcohol containing diet. Results show that alcohol consumption during pregnancy even with protein, mineral and vitamin enriched diet may affect maternal and fetal health, and alter integrin receptor signaling pathways in the fetal cerebral cortex disturbing the development of fetal brains.
Alcohol consumption during pregnancy disturbs the normal development of fetus causing wide range of structural and behavioral problems in children commonly known as fetal alcohol syndrome (FAS) [1]. Reduced birth weight and mental deficiencies are the hallmarks of FAS that persists even in adolescence [2]. Low birth weight is estimated to be about 10% of health care costs for children [3] and is linked to obesity [4], hypertension, diabetes mellitus [5], and reduced fertility [6] later in life. FAS is considered to be the leading cause of mental retardation in the Western world [7].
Normal functioning of brain requires optimum migration of neurons in the fetal brains [8,9]. Several studies with Long-Evans rats now show that the migration and proliferation of fetal cerebral cortical neurons is altered by prenatal alcohol even when the alcohol in the protein- and nutrient-enriched liquid-diet is increased in a gradual and step-wise manner [10–13]. In vitro studies with slice culture of fetal cerebral cortex from Sprague-Dawley rats reveal that alcohol may alter the expression of integrin subunits that are involved in the migration of neurons [14]. A recent study shows that acute alcohol exposure during early gestation alters transcript levels of integrin subunits and extra cellular matrix (ECM) proteins in the fetal head fold of mouse in a genotype dependent manner [15]. Yet, the effects of prenatal alcohol on maternal diet consumption, and the weights of mother, fetus and placenta in Long-Evans rats, a species extensively used for studying effects of maternal alcohol on fetal brains, are not known. Moreover, the effects of chronic prenatal (in vivo) alcohol on the expression levels of molecules involved in the integrin signaling in the fetal cerebral cortex are currently not known. Therefore, in the present study an established rat model of FAS was used to examine the effects of maternal alcohol on the body weights of mothers and fetuses as well as on the expression levels of molecules involved in the integrin signaling in the fetal cerebral cortex.
All procedures performed on rats, including the use of diet regimens for the studies, were according to an animal protocol approved by the University of Mississippi Animal Care and Use Committee.
Pregnant Long-Evans rats were obtained from Charles River (MA). Animals were acclimated on regular chow and water in the laboratory animal facility of The University of Mississippi Medical Center in a 12 h light (6 AM to 6 PM) and dark cycle. On gestation day (GD) 5, animals were weighed and divided into control and experimental groups each consisting of animals of similar weight range with average weight of 256 g. Each group consisted of total 8 pregnant mothers. Starting on gestation day 6 morning, food and water supplies were replaced with 110 mL liquid-diet F1265SP (Bio-SERV, French Town, NJ, USA) in graduated glass feeding tubes. F1265SP is a Lieber-DeCarli diet [16] enriched with proteins, vitamins and minerals. The components of this diet included, Corn Syrup, Casein, Olive Oil, Maltodextrin, Cellulose, Mineral Mix, Corn Oil, Suspending Aids, Safflower Oil, Vitamin Mix, L-Cystine, Choline Bitartrate and DL-Methionine.
Alcohol (ethanol) was added into the diet for the experimental groups of animals. Maltose-dextrin was added into the control diet to ensure that the control and alcohol-containing diets were isocaloric. Concentration of alcohol (v/v) in the diet was increased with the gestation and maintained at 2.2% during GD 6 & 7, 4.5% during GD 8, 9 & 10, and 6.7% during GD 11 to 17 or 19 according to a previously published protocol [10] that has been used to examine the toxic effects of maternal alcohol on fetal brains. Because in this protocol significant differences in the migration of only late generated neurons were recorded between the pair-fed (Chow and water) and control Liquid-Diet (minus alcohol) groups, the pair-fed group was not included in our study. In addition, the Chow and water group of control was excluded from our study because no differences in the results between this group and liquid diet with no alcohol group is reported in other studies [17,18], The volumes (mL) of diet consumed every 24 h by individual rats were recorded in the mornings (9 AM) and freshly prepared diets were supplied in clean tubes.
On GD18 or GD20 morning (9 AM) animals were anesthetized with isoflurane (Abbott Labs., Chicago, IL, USA) and weighed. The abdomen was opened with a pair of scissors, and the diaphragm ruptured for the collection of blood from the heart with a syringe. Blood (0.5 mL) was transferred into an anticoagulant blood collection tube. Alcohol concentration in the blood (mg/dL) was determined by head space gas chromatography.
The uteri were dissected out and transferred into a tray containing chilled phosphate buffer saline (PBS). Fetuses and placentas were collected from uteri and transferred into plastic Petri dishes containing chilled PBS. Fetuses and placentas were separated with a pair of scissors, soaked with facial tissue papers to remove extra PBS and weighed. Number of fetuses per mother was recorded. General morphology of fetuses (such as presence of cleft) and placentas were examined visually.
Heads from gestation day 18 fetuses were transferred into chilled neuron culture medium DMEM/F12 (Invitrogen, Carlsbad, CA, USA) for the removal of brains. Cerebral cortices were dissected out and meninges removed (Figure 4A). Cerebral cortices from 2 fetuses per mother were pooled and subjected to homogenization in the TPER solution containing Halt proteinase inhibitor (Thermo Fisher Scientific, Inc., Rockford, IL, USA). The homogenates were centrifuged at 90 g and 4 °C for 10 min. The clear supernatants were stored at −20 °C. Aliquots of supernatants were subjected to protein assays using a BCA protein estimation kit (Thermo Fisher Scientific) as described earlier [19]. Fetal cerebral cortical proteins (15 μg) from control (liquid-diet only) and alcohol (liquid-diet plus alcohol) exposed mothers were subjected to polyacrilamide (10%) gel electrophoresis and Western blotting as described earlier [19]. Membranes were exposed to primary antibodies (Table 1) in blocking solution overnight at 4 °C, Membranes were washed and exposed to peroxidase-conjugated secondary antibodies (Jackson Immunoresearch, West Grove, PA, USA) (100 ng/mL PBS) for 1h at room temperature. Bands representing respective antigens were detected using a Chemiluminescent detection system and Amersham Hyper film ECL (Both from GE Healthcare Ltd., Buckinghamshire, UK). Optical densities of bands were determined using ImageJ Software as described earlier [19].
SPSS software (SPSS Inc., Chicago, IL, USA) was used to determine the mean and the standard errors of mean of diet volume; maternal, fetal, placental weights and optical densities of bands. ANOVA (Post hoc analysis) was conducted to determine the significance of differences of mean + standard error of mean values obtained from control and alcohol-exposed animals at p < 0.05.
Pregnant animals consumed ~100 mL control liquid-diet during the whole course of diet regimen except the first day (GD6) of transition from the diet containing chow and water into the liquid-diet regimen, when the consumption was less (~82 mL). Volume of liquid-diet containing 2.2% alcohol was similar to the control diet (p > 0.05) on the first day of liquid-diet regimen. Afterwards and throughout the diet regimen, the consumption of alcohol-containing diet mostly remained significantly lower (p < 0.05) than the control diet. Consumption of the diet with 2.2% alcohol during GD7 was 13% lower than the controls; whereas consumption of the diet with 4.5% alcohol during GD8-GD10 was 8–13% lower than the control diet. When the alcohol concentration in the diet was raised to 6.7% during GD11 to GD19, consumption of the diet dropped even further and was 11–30% lower than the control diet. The total volume of the alcohol-containing diet consumed by the pregnant rats during gestation days 6 to 19 was about 17% lower than the control diet. Volumes of the diets consumed by the control and alcohol groups of pregnant animals are shown below (Figure 1). The calories ingested by the control and alcohol groups of pregnant animals are shown in Figure 2.
The mean blood alcohol concentration in mothers at GD18 (n = 8) and GD20 (n = 8) were ~49 and 61 mg/dL, respectively. The maximum blood alcohol levels were 126 and 155 mg/dL on GD18 and GD20, respectively.
Weights of pregnant mothers on alcohol were significantly lower (p < 0.05) than the weights of those on control diet (Figure 3A). Weights of fetuses at GD18 and GD20 from mothers on the alcohol diet were also lower compared to those from mothers on the control diet at GD18 and GD20 respectively (Figure 3B). Prenatal alcohol increased the weights of placentas isolated from GD18 and GD20 mothers (Figure 3C). Accordingly, the fetal/placental weight ratio at GD18 and GD20 decreased due to chronic alcohol ingestion during gestation (Figure 3D).
The number of fetuses from mothers on control or alcohol containing diet varied from 12 to 14, and the means + standard error of mean values of fetal numbers were not significantly different between the two groups. All fetuses born from control and alcohol fed mothers were alive. No obvious differences in facial features (such as cleft or abnormal eye), limbs, tail and body were found between the control and alcohol-exposed fetuses at GD18 and GD20. Placentas from the control and alcohol treated groups had no gross morphological differences.
Expected molecular weight bands representing β1 (130 kDa), α3 (135 kDa), PLC-γ1 (155 kDa), PLC-γ2 (155 kDa), N-cadherin (140 kDa), β-actin (42 kDa) and GPDH (36 kDa) were detected in the fetal cerebral cortices using the Western blotting technique (Figure 4B). The antibody against α6 integrin subunit recognized two electrophoresis bands from the fetal cerebral cortex, an expected 127 kDa band and an additional band at 150 kDa, which may represent two splice variants of this subunit [20]. Analysis of band intensities revealed that gestational alcohol increased the expression of β1 integrin subunit in the cerebral cortex of GD18 fetuses. Contrarily, the expression level of α3 integrin subunit was decreased significantly (p < 0.05). The expression level of α6 integrin subunit was also decreased in the fetal cerebral cortices exposed to alcohol; however this decrease was not statistically significant (p > 0.05). The expression levels of phospholipase C (PLC)- γ1 were not different between the control and alcohol exposed cortices and the PLC-γ1 was not phosphorylated at tyrosine 783 (data not shown). The authenticity of Phospho-PLC-γ1 (Tyr 783) antibody was verified in a cancer cell line (data communicated elsewhere). Remarkably high levels of PLC-γ2 were detected in the fetal cortices that decreased significantly (p < 0.05) due to prenatal alcohol exposure (Figure 4B and Figure 4C). The expression levels of N-cadherin, β-actin and GPDH were not significantly different between control and alcohol-exposed groups of fetal cortices.
Results presented here show for the first time that (i) the pregnant Long-Evans rats consumed significantly less of a protein fortified liquid-diet (F1265SP) when the alcohol in the diet is increased in a step-wise manner and (ii) that in this species of rat, the expression of molecules involved in the integrin signaling are significantly altered in the cerebral cortices of fetuses exposed to alcohol during gestation even in the absence of obvious morphological defects in the offspring. These results show that the pregnant Long-Evans rats on the liquid-diet containing alcohol are undernourished and that the fetal cerebral cortical development may be impaired due to disturbances in integrin receptor signaling.
High protein concentration or step-wise increase of alcohol in the liquid-diet is known to increase diet consumption and maintain weights of pregnant Sprague-Dawley rats [17,18]. Therefore, the suboptimal consumption of liquid-diet enriched with proteins, minerals and vitamins by the pregnant Long-Evans rats, even when alcohol in the diet was increased in a step-wise manner, suggests that the consumption of liquid-diet with alcohol—and thus the outcomes of prenatal alcohol—may differ between strains of rats. Maternal genotype [21], genetic differences in the extent of taste aversion for alcohol in the diet [22] or an increase in the sleep-time caused by the alcohol [23] in these strains may determine the extent of maternal alcohol consumption during pregnancy. Genetics may also determine the effects of undernourishment on the mother and offspring. More severe reduction in maternal weight gain, fetal body weight and increased placenta weights are associated with dietary restrictions in genetically distinct C57BL/6J versus A/J mouse strains [24,25]. Moreover, in the context of the proposed role of maternal genotype on the outcome of dietary restriction in children [26], the present findings suggests that the Long-Evans rats may be genetically susceptible for the malnutrition-induced loss of body weight.
A single intra-peritoneal injection of high alcohol dose during gestation reduces fetal weights in C57BL/6J mice [27], and alcohol in the diet is shown to reduce intrauterine growth by suppressing insulin-like growth factor-1 and reducing the transfer of 2-deoxy glucose to the fetus via the placenta [18,28]. On the other hand, malnutrition itself may affect the weight of offspring by reducing Insulin like growth factor expression [29], and by increasing alcohol-induced feto-toxicity mediated by the reduced liver alcohol and aldehyde metabolizing enzymes in the fetus [30,31]. Therefore, both the undernourishment caused by the decreased consumption of liquid-diet containing alcohol as well as the direct effects of alcohol may be the contributing factors in reducing the weights of pregnant Long-Evans rats.
Increased placental weights and the decreased fetus/placenta ratios (Figure 3) observed in the alcohol-exposed mothers were also found in earlier studies with Wistar, Sprague-Dawley, and albino rats [32–35]. So the increase in placental weights in rats on alcohol-containing diet may be a common phenomenon irrespective of the strains. Placental weight is also reported to increase due to malnutrition itself; and this concurrence is interpreted as a compensatory mechanism to reduce the effects of maternal under-nutrition on the fetus [36].
The lack of difference between the litter size of the control and alcohol fed groups and the absence of dead fetuses in this study, as opposed to previous studies [37,38], may in part be due to the low concentrations of alcohol in the diet during the first trimester. This may also be due to the delayed start of alcohol liquid-diet regimen, from GD6, in this study as opposed to the time of conception in other studies [37,39], which may reduce embryo implantation and cause fetal resorption decreasing the litter size in alcohol-exposed mothers.
Even though visual examination of the fetuses from Long-Evans rats on alcohol showed no gross morphological changes, results of Western blotting studies revealed that the prenatal alcohol in the liquid-diet influenced molecules that are directly or indirectly involved in the integrin receptor signaling. This included heightened expression of integrin subunit β1 (p < 0.05) and decreased expression of α3 subunit (p < 0.05), that are required to form the functional β1α3 integrin receptor for the migration of neurons in fetal brains [40]. In previous studies, high concentrations of ethanol (400 mg/dL) in culture is shown to increase the expression level of β1 integrin subunit in B104 neuroblastoma cells [41] and in cerebral cortical slices of fetal (GD 17) Sprague-Dawley rats [14]. The latter study also showed elevated levels of α3 integrin subunit in the cortical slices cultured in presence of high concentration of alcohol for 6h but not on 12 h. This discrepancy in the α3 integrin subunit expression data between the previous [14] and our in vivo study could be caused by differences in the concentration of alcohol and treatment times (6 and 12 h) used in the in vitro study and those attained in the blood of mother during chronic exposure to alcohol. Indeed, an in vivo study shows that acute alcohol intoxication on gestation day 8 lowers α3 integrin subunit transcript levels in the head fold of a substrain of C57BL/6 mice [15]. This later study also confirmed that the integrin receptor subunits and ECM molecules in the fetal brains are the direct target of alcohol, i.e. without malnutrition as a confounding factor. Nevertheless, all these studies emphasize that alcohol-induced change in the expression levels of molecules in the fetal brains varies with the strains of rodents as well as with the time, dose and pattern of alcohol exposure.
Phosphoinositide-specific phospholipase C (PLC) isoforms play a critical role in mediating integrin receptor signaling by hydrolyzing phosphatidylinositol 4,5-biphosphate (PIP2) to generate second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) [42]. Of several PLC isoforms, the PLC-γ1 is known to play significant role in integrin signaling and is activated by the epidermal and platelet derived growth factors at tyrosine 771, 783 and 1,245 positions [43]. Data presented here show that the prenatal alcohol neither alters the expression levels of PLC-γ1 nor activates it by phosphorylation of tyrosine at position 783. Instead, the prenatal alcohol decreases the expression levels of PLC-γ2 isoform in the cerebral cortices, an isoform that is known to influence integrin signaling in osteoclast [44]. These results indicate that prenatal alcohol may alter integrin mediated signaling and functions in the developing cerebral cortices by altering the expression of β1 and α3 integrin subunits, and PLC-γ2. Results from this study also show that the expression levels of N-cadherin, the activity of which is regulated by β1 integrin mediated signaling [45] and which is involved in the cerebral cortical development [46] was not altered by alcohol in the diet. This, however, is consistent with the recent findings [15] that shows that the transcripts of cell-cell adhesion molecules 'Cadherins' are not altered by alcohol.
Signaling mediated by the integrin receptors is indispensable for the normal development of the brain [47]. It is required for the development of the radial glial fibers and neocortical layers [48]. In the developing cerebral cortex, the expression of β1 and α3 integrin subunits are detected throughout the cortical wall, whereas the expression of α6 subunit is restricted to the ventricular zone and the cortical plate [47]. Both α3β1 and α5β1 integrin receptors on neuronal surface are required for the radial migration of neurons [49,50]. Therefore, altered levels of β1 and α3 integrin subunits and the signaling mediator PLC-γ2, caused by gestational alcohol, are expected to change the neuronal positioning causing heteropias [51] and disturb the formation of cerebral-cortical structures.
The peak values of blood alcohol concentrations in the Long-Evans rats (126–155 mg/dL) were in accordance to the values reported earlier with similar diet regimen in this species [10]. Since, the alcohol level in humans on a chronic alcohol diet are known to reach up to 400 mg/dL [52], the altered maternal, fetal and placental weights and the expression levels of molecules in the fetal cerebral cortex observed in this study, indicate that alcohol at half of the concentration achieved during chronic drinking in humans may affect both the health of the mother and the cortical development of the fetus.
It is well known that human alcoholics become calorie restricted—they eat less as they drink more. Our experiments, by reproducing this effect in an animal model (Figure 2), has more closely fitted a human situation. The absence of obvious developmental defects in the offspring of Long-Evans mothers on chronic alcohol during pregnancy as opposed to other strains of rat [37,53,54] show that the Long-Evans rats may be used as a genetic variant for understanding the mechanisms of neurobehavioral problems that may occur in children in the absences of obvious signs of FAS [55]. Moreover, our study provides the first report of chronic in vivo modeling of the effects of alcohol on molecules regulating integrin signaling as previous reports on the changes in the expressions of integrins subunits in fetal brains by alcohol were performed either on brain slices in vitro [14] or in an acute in vivo exposure model [15]. Future studies are now warranted to dissect the effects of malnutrition and alcohol in pregnant Long-Evans rats. This will be conducted by comparing the results from a group of animals provided with the same volumes of liquid-diet with no alcohol that is consumed by a group of animals on the liquid-diet with alcohol at different gestation days.
In the final analysis, results presented here and those published earlier [14,15,56] demonstrate that integrin-ECM interaction mediated cell signaling is the crucial target of maternal alcohol exposure in the fetal cerebral cortices and may occur in the absence of obvious developmental defects of FAS. Because integrin receptor signaling regulate proliferation and migration of cells in the fetal brains [40,47,48], the prenatal alcohol-mediated changes in the expression levels of molecules involved in integrin receptor signaling would cause wide range of problems during the organogenesis of the fetal brain structures. It is now apparent that inhibiting the effects of prenatal alcohol on the integrin receptor signaling in the fetal brains may reduce the severity of FAS.
This study was supported by a Small Grant to UK Rout from the Center for Psychiatric Neuroscience, UMMC, Jackson, MS 39216, USA. The blood alcohol concentration determination service of the toxicology laboratory, Department of Pathology, UMMC is acknowledged. Valuable suggestions by John Duley (School of Pharmacy, The University of Queensland, Brisbane, Qld, Australia) in writing this manuscript are highly appreciated.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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The package implements models to analyze site occupancy and count data models with detection error. The package development was supported by the Alberta Biodiversity Monitoring Institute (ABMI) and the Boreal Avian Modelling (BAM) Project.
|Peter Solymos, Monica Moreno, Subhash R Lele
|Date of publication
|2014-05-16 16:57:03
|Peter Solymos <firstname.lastname@example.com>
AUC: AUC ROC plot for fitted models
bootstrap: Do bootstrap and extract bootstrap results
cmulti: Conditional Multinomial Maximum Likelihood Estimation
convertEDR: Conversion between truncated and unlimited effective...
databu: Simulated example for abundance model
datocc: Simulated example for occupancy model
detect-package: Analyzing Wildlife Data with Detection Error
hbootindex: Hierarchical bootstrap indices
internals: Internal functions
load_BAM_QPAD: Load BAM QPAD parameter estimates and support functions
oven: Ovenbird abundances
svabu: Binomial-Poisson and Binomial-ZIP models with single visit
svocc: ZI Binomial model with single visit
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Nike+ GPS improves social features, adds auto-calibration
Nike has released a minor update to its GPS-based run tracking app adding improvements to its social media integration and a new auto-calibration feature. Nike+ GPS 3.1.1 now allows users to include their pace in their Facebook and Twitter posts and provides the option to edit Twitter messages before posting. The app now also makes use of Push Notifications for keeping track of ongoing Nike+ Tag games and allows users to link the music play/pause button to start and pause runs. The update also adds an auto-calibration feature to assist users with calibrating their runs to actual distance and improve accuracy. Users can also now register or login using Facebook or Twitter accounts in addition to their Nike+ account. Nike+ GPS is available from the App Store for $2.
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text: 150 Captures: Cosmopolis 'Making of' (Featured on French Blu Ray DVD) Posted on 09/27/2012 by Mel · Leave a comment 150 caps from the Cosmopolis 'Making of' featured only on the French Blu Ray DVD! (You can order your DVD HERE and the Blu Ray HERE) source: via Share this:TweetShare on TumblrPrint Related Popular Posts & Pages ***SPOILER ALERT*** Camp X-Ray Kristen Stewart's Playlist Picks! Kristen's Interview with Style.com [Rosabotanica Press Day] New Edward and Bella Still from Breaking Dawn Part 2 New/Old pics of Rob in London 2009


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Nowadays most of the families have a computer. If you are moving to a new home, you want to ensure that your computer arrives safely with you. There are few extra steps you need to take when moving a computer.
How to Pack a Computer
You may rent a moving truck for in-town or long distance move and it can save you in moving expenses. However, besides the daily charges, there are extra charges such as fuel, mileage, insurance and etc that need to be calculated properly before renting a truck.
How Much Does It Cost to Rent a Truck
Self Storage companies restricted by certain limits, you are not allowed to store everything in storage units. For those people who are planning to rent a storage unit, here is a list of the items that are not allowed to be stored.
What You Are Not Allowed to Store
There are many cheap service providers out there, but one thing you have to remember is that moving is never a gamble. These cheap companies can save you money, but before hiring one make sure they meet your and State requirements.
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Originally published 09/15/2015
Saudi coalition bombs a historic site in Yemen and destroys it
Baraqish in Yemen had been restored by the Italians. Now it's in ruin.
Originally published 09/10/2015
The religious and cultural heritage being ruined by Yemen's war
The roster of antiquities damaged in the war in Yemen runs long.
Originally published 04/03/2015
Nasser's Ghost Hovers Over Yemen
Jesse Ferris
In 1962 Egypt invaded Yemen and Saudi Arabia intervened. Is history repeating itself?
Originally published 03/30/2015
Why Yemen Matters
Daniel Pipes
The Middle East witnessed something radically new two days ago, when the Kingdom of Saudi Arabia responded to a plea by Yemen's president and led a 10-country coalition to intervene in the air and on the ground in the country.
Originally published 10/09/2014
Radicals Have Taken Over Northern Yemen. Should We Be Worried?
Juan Cole
The threat to world trade in the Suez Canal has been ignored by the mainstream media in the US.
Originally published 02/12/2013
Will Washington Ever Learn from Its Past Mistakes? Not Likely.
Tom Engelhardt
Satellite image (from Bing) of what could be the two-year-old U.S. drone base in Saudi Arabia.Originally posted on TomDispatch.com
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ALL >> Business >> View Article
Are You Considered A Trend Setter For Fashion?By Author: Heidi Maxwell
For someone who doesn't usually think about fashion, shopping can be frustrating and confusing. If you have gone there more than once and didn't care for it, you could use some assistance. Thankfully, the below article gives you excellent tips to make shopping easier for you.
Accessories are your best friend. You can take any old outfit that would be quite boring or plain on its own and dress it up with amazing accessories. Add the perfect necklace or brooch to a little black dress, and you've taken that LBD from average to awesome in two seconds flat.
Buy a variety of jewelry pieces. If you have a good number of colors and styles, you will always have something that matches your outfit. You then will not have to worry about purchasing jewelry to match every outfit that you buy. Think variety and you will have more than one option for an outfit.
When selecting a dress, choose one that has a vertical (rather than horizontal) pattern. This makes you appear longer rather than wider, giving the illusion that you are slimmer than you really are. Also, seek out a piece that has an empire waist, as it makes it seem like you have a smaller waistline.
If you own a beauty kit, do not store a ton of makeup in it. Instead, pick your desired products in colors that are appropriate for the season. Think about what you will need from the time you get up until you go to bed. Also, remember that makeup has a shelf life and should be replaced when it has gone bad. There is also the potential for germ growth if a product sits too long.
A good fashion tip is to learn how to develop your own sense of style. You don't want to just mimic somebody else and copy their style. Think of what's important to you such as comfort or flair, and then slowly build on that so that your fashion sense is unique to you.
A great fashion tip is to start shopping at thrift stores for some of your clothing. You can often find really unique clothing at thrift stores and you don't have to pay an arm and a leg either like you would if you bought new clothes at some big department store.
If you notice your hair frizzing when it is dry, try applying just a little leave-in conditioner from the top to the bottom of your hair follicles. If your hair is very curly, use a serum instead and start around the middle of your hair rather than at the crown. These tactics will help to smooth things out and help you to look your best.
Something as simple as a new pair of blue jeans can really add to your fashion portfolio. There are dozens of cuts, sizes, and fits of jeans to choose from in each store you go to. Don't let the huge assortment overwhelm you. Classic styles, like straight leg and boot cut, are the way to go. They never go out of style and always look great.
With the fashion advice from this article, you can take on the shopping! Follow these tips to start feeling and looking proud of yourself. Soon, you'll look great and feel even better.
If you would like to know more about the latest fashion today, you can find more information on the great
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One that isn't reminiscent of a rehashed version of your class 8 History textbook. As part of their centenary year in India, Oxford University Press has re-issued Mahatma Gandhi's, My Early Life, first published in 1932.
On the eve of his 143rd birthday, this book, which was written initially, by Gandhi while in jail, was arranged and edited by his secretary, freedom fighter and nationalist writer, Mahadev Desai. Lalitha Zachariah, a Gandhi scholar, has annotated this edition. Sourav Chakravarthy's line drawings add depth and liveliness to every chapter. A master stroke, this, to draw in the young, impatient reader.
One of the earliest books to be developed by OUP in India, the original is a concise version of Gandhi's autobiography. It looks at his teenage aspirations, confessions, his pursuits in London as well as his austere choices in South Africa, all of which are a window into his transition from Mohandas to the Mahatma.
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Photos: SRK, Tamannaah Bhatia, Varun Dhawan at Mehboob Studio
Birthday special: Sushant Singh Rajput's journey from TV to films


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This is Tyler and I am a web developer with some pretty impressive skills. I was just looking at your site and you have some good elements, but it can definitely be much stronger and I would like to help you to improve it. My overall goal isn't just to build websites, but help my clients realize their vision for their business. Would you be interested in getting a consultation for a site redesign? Let me know if you're interested. I'd really love to work on your site. Hope to hear from you soon. Thanks! Tyler Webb
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National Park Service banned from tweeting after anti-Trump posts
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Monthly Archive for October, 2012
MSM on Benghazi: "not optimal"
The question that starts my column today: "Is there a mainstream media cover-up of an Obama administration cover-up in the wake of the 9/11 Benghazi attack that left four Americans dead? …
What Prop. 34 folks don't want you to know
Why wouldn't the League of Women Voters want to hear from both sides on Proposition 34? That is the subject of my column today on the League's one-sided approach to the ballot measure to end …
Libya emails fingered terrorist group on 9/11
I share the frustration of readers who want to see more coverage on the Benghazi attack and probable coverup and less of the "binders," "bayonets" and other silly stories masquerading as news during …
Don't shoot the moderator
With tonight's debate, voters will have had their third chance to see Mitt Romney and Barack Obama face off. They also will have seen four different moderators. All three so far have endured their …
Jerry Brown's office insists California did not fudge jobs data
There's some good news for California. The state still has the third highest unemployment rate in the country, but the unemployment has dropped to 10.2 percent. But all is not celebratory in …
Larry Ellison: big design sinks ships?
What is it about rich guys and big, bad boats? As The Chronicle's Kevin Fagan and Justin Berton reported, AC72, the over-designed Oracle catamaran capsized, crumbled and was dragged out to sea …
Would Obama be better if he had lost a big election?
I find myself wondering what kind of a president Barack Obama might have been if he had waited to run for office and not decided to run for the White House almost immediately after winning his U.S. …
Veep Debate — Paul Ryan won
Here's why Paul Ryan won. The rap on Paul Ryan has been that he is an inflexible ideologue. What viewers saw was a running mate who talked about working with Democrats, about Romney's record of …
Nancy Pelosi says she read Obamacare bill
House Minority Leader Nancy Pelosi came to the Chronicle for an editorial board meeting Wednesday. I used the opportunity to ask Pelosi about her most famous and quoted statement from 2010. On March …
Ross Mirkarimi remains San Francisco's Sheriff
As of this writing, three supes — Christina Olague, David Campos and John Avalos — voted against the City Ethics Commission vote to remove Sheriff Ross Mirkarimi . Jane Kim later joined the three to …


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ALL >> Home-Based-Business >> View Article
How Online Lead Generation Works
Total Articles: 23
For some reason folks seem to believe starting your own home business is supposed to be cheap. Sometimes this is actually the scenario. For example, if you're starting up a writing business of your own, you already have almost all of what you need. You have a laptop or computer and also the web. But let's say you wish to take on something else. What if you'd like to begin your own eBay company? What if you need to begin with a typical sales business? What if you need to begin your own affiliate marketing business? Just about every internet business out there needs some sort of start up funding. But what if you're broke? Use a number of the following solutions to help yourself acquire some start up funding.
Is there anything in your house you could put up for sale on eBay or in a garage sale? This may act as the motivation you've been needing to clean out your garage or attic. While you might not make a lot of money by doing this, you could raise enough dough to do some small start up things like purchasing your domain name or hosting for your website. What you sell is up to you but you might even manage to earn enough capital to outsource a few of your marketing duties or to buy something that you can re-sell for more funds.
What do you think you're good at? Is there some thing that you are proficient at that you can do quickly--if there is, start an offer at fiverr.com. This way you'll be spending your time doing something you enjoy and that you will be good at. You may also get to do this for five dollars a pop. Even if only 10 individuals a day purchase your offer, that is still $250 for 5 days work. That isn't really that bad for producing extra spending cash. The good thing about most of these sites is that you don't have to promise people the moon and you can be creative. It's merely a way to earn a quick five dollars.
Go offline. There are lots of things you can take on to help earn money--things that don't involve your computer. You might take on dog walking or grooming work. If you know how to build a fast website, you can provide quick websites to firms that don't yet possess one or that have something that isn't good. This only takes some time and cold calling and you might make lots of money. Hand out fliers. Sign up to gather signatures. There are a lot of great "part time" and "etc" gigs on Craigslist that can help you make money without your having to spend tons of time or money on them.
There are a lot of ways to make money. When you open up your own web business, though, you're not just generating money, you're setting up your career. This type of project typically requires a commitment of time and funds. Use these pointers to get your enterprise open and in business. Don't forget to have fun when you earn these funds!
I am a Video Marketing Artist that has worked in the profession for the past 11 years.
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Author: Just Link


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This is going to be a long, hot summer - and I don't mean the temperature. The debt, deficit and spending fight on Capitol Hill is intense. If the government does not increase the debt limit in August, the United States will default on its bills. The Republicans want Washington to cut its spending to match the revenue (taxes) coming in, and the Democrats want to increase our taxes. Unless the two sides come together with a compromise, our country goes into default.
As far as state parks are concerned, being boring shouldn't always necessarily be seen as a bad thing.
I own a vacation home in Dawson County – Big Canoe to be exact. Every year, we get a bill for property taxes and it is paid promptly.
I was speaking to a member of the General Assembly last year in the wake of a series of ethics scandals that led to the former speaker of the House resigning, and he said, "We've passed an ethics bill that greatly expands the disclosure of campaign finance and lobbyist spending."
Gov. Nathan Deal has had a welcome change of heart about another hike in the state tax on gasoline after reaping a windfall from a formula-induced jump of nearly 28 percent less than two months ago.
The recent rains certainly have been welcome. Since we finally got some precipitation, it is a good time to bring up the problematic relationship between litter and stormwater.
After hearing arguments last week from a coalition of immigration attorneys and civil-rights organizations seeking to block implementation of Georgia's new immigration law (HB 87), which went into effect July 1, U.S. District Judge Thomas Thrash issued his ruling Monday afternoon.
Oh, I love it when I have happy news! It looks like after a wait that seemed to go on forever Josh and I will finally close on our house this week.
The Coastal Regional Commission recently unveiled a tentative plan to retrofit 13 of its coaches to run on compressed natural gas or propane. The change, of course, was designed as a cost-saving measure as alternative fuels cost between $1.75 and $2 per gallon compared with gas prices of around $3.50 per gallon. But let's not forget the strategy's added benefit of being environmentally friendly. The CRC should be commended for using energy conservation to help save money and the planet, and more mass transit systems should follow the commission's lead - it may not be all ...
Editor, I feel that it is going to be a big economic mistake if the U.S. government takes away Social Security from those seniors older than 65 and who are on Social Security already. Just talking about it already has hurt the economy. Seniors have stopped buying major items because they are scared the money won't be there to pay for them. Major items usually are bought on credit. This means businesses like Lowe's and Walmart will lose business and have to cut labor hours or lay off employees. This adds to the unemployment and underemployment problem ...
Each year, more than three trillion miles are traveled on America's roads, with a considerable amount of those - more than 113 billion -- occurring right here in Georgia. Our location and excellent road network means Georgia serves as a prime connecting route for vacationers and for freight movement. Georgia is the main route by which tourists from all over the USA and Canada reach popular year-round southern vacation destinations. And when we add the increasing numbers of tractor trailers traveling throughout the state, our roads are usually crowded.
This morning I was staring at my biscuit, wondering if it was a Christian biscuit or not.
Sixty-five new laws took effect in our state on Friday. New laws like immigration and Sunday alcohol sales have gotten much media attention since they were approved by lawmakers and signed by Gov. Nathan Deal.
Editor, As with all articles dealing with taxes, I read the "MidCoast Regional Airport finalizes budget proposal" with great interest.
July 4, 1776. On this date, we became "We the People." Thirteen colonies declared their independence from England, becoming the United States of America; and it is the only country to be organized where the people are in charge, not the government. The people of this country do not answer to a king, emperor or emir.
The mayoral election seemed a million miles away on Friday night at the Spring County Fair. With "Silver Tongue" firmly entrenched in his role as ...
On Monday, the Georgia General Assembly began the 2017 session and I am honored that you place trust in me to represent you in the ...
Mike Riddle was a pretty good Long County correspondent for the Coastal Courier for a lot of years.
Editor: As the city turns. In our last episode, the suspense was high as the city council reprimanded two outstanding employees unjustly. The council also ...
If you are still having trouble firming up some New Year's resolutions that you can actually keep, consider recycling.
Editor: The use of Naloxone can save a life. Naloxone, also known as Narcan, is an opioid antagonist used in opioid overdoses to counteract the ...
Henry Ford converted a 1937 For pickup truck into a fire truck to be used in Way's Station, now Richmond Hill.
The morning following "The Great Valley Runoff" was perhaps the oddest moment of my growing-up years. It was as if we were surrounded by fog ...
My loyal readers, both of them, possibly remember that I've long championed some outside-the-box ways to reduce the deficit before we're overwhelmed with ...
The year 2016 is over, but I want to start 2017 by looking back at some of the positive things from last year.
Donald Trump was supposed to take over the Republican Party, but the question going forward will be whether the Republican Party takes over him.
As we end 2016, we pause to consider how much have we emancipated?
Rick Downs needs your help now.
Dear Cameron Charles Yarbrough:
Editor: Thank you to Mrs. Patricia Fleming of the Kingdom Church of Christ, formerly the Jr. Church of Christ, for successful free Christmas dinners to ...
© 2017 Hinesville Publishing - All Rights Reserved
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Monahans Bars
About Search Results
YP - The Real Yellow PagesSM - helps you find the right local businesses to meet your specific needs. Search results are sorted by a combination of factors to give you a set of choices in response to your search criteria. These factors are similar to those you might use to determine which business to select from a local Yellow Pages directory, including proximity to where you are searching, expertise in the specific services or products you need, and comprehensive business information to help evaluate a business's suitability for you. "Preferred" listings, or those with featured website buttons, indicate YP advertisers who directly provide information about their businesses to help consumers make more informed buying decisions. YP advertisers receive higher placement in the default ordering of search results and may appear in sponsored listings on the top, side, or bottom of the search results page.
1. Jakes Lounge
2524 W Sealy AveMonahans, TX 79756
2. Thirst T's
1900 S Stockton AveMonahans, TX 79756
3. I Dont Care Bar & Grill
4715 S Highway 18Monahans, TX 79756
4. Bar-H Steakhouse
Food has such a great flavor! Have yet to have something I didn't like. Great homemade cooking! Chili is the most amazing chili I have ever had i…
5. Fred's Bar-B-Q
305 N Bruce AveMonahans, TX 79756
6. Pappy's Bar-B-Q
1901 S Stockton AveMonahans, TX 79756
7. Cork & Pig Tavern
Very good. Good food, good atmosphere, good service. Enjoyed this place very much.
8. Sook's Place
820 Ward StPyote, TX 79777
9. The Archway Club
Serving the Monahans Area.
10. Pyote Hideaway
101 Hwy 115Pyote, TX 79777
11. The Hemingway
3952 E 42nd St #OOdessa, TX 79762
Showing1-11 of 11results


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Don't read too much into the results of these sample spins! The method of picking rules is yet to be tweaked to its full potential, and until a good selection of rules have been added, it's rather meaningless.
Once the Rule-O-Matic has been spun for a final time, the authors of the selected rules will be contacted in order to add clarifications and last minute twists. They won't be allowed to do anything drastic, and to limit their advantage of a heads up, they won't know for sure which of the rules atually were selected. (For example, they may be notified that one of three specific rules have been selected.)
Here is a sample spin, with the following rules being chosen:
Genre requirements
Be a crackpot (Good)Story and gameplay elements must focus around strange, demented religious elements of your own invention.
Technical requirements
There are 2 technical requirements:
Second Person Sh...what!? (Great)
Your game must be in Second Person - this means you're seeing from the eyes of a character you don't have full control over, or you could be controlling a character who indirectly controls the main one. There must be, anyway, someone between the player and the main character.
As examples, the character's head could be in a jar that must be transported separately from his body, or the character could be seeing from his enemy's point of view, but controlling his own character.
Weakening (Good)
The player must become less powerful over time.
Let the player start with good resources that degrades or gets lost as he makes progress through the game.
Example: If you make an RPG, you can let the protagonist lose strength instead of making the enemies stronger.
Artistic requirements
There is 1 artistic requirement:
World Map (OK)
You must have a world map:
This may be in street-fighter style where you are shown flying to the country where you will be competing against another player. You could also have a world map that has lots of challenges are shown, and you can select where to go to next. Another possibly the game has a linear path, but you are shown on a world map how far you have progressed.
Bonus rules
There is 1 bonus rule:
Act of Efficiency (Good)
You may opt out of one other rule if the ZIP file of your entry is less than 150KB (153600 bytes)
Other Important Info
All entries must comply with all requirements except where nullified by Act of Dog.
All entries must be submitted on or before 12:00 UTC on Monday 25th July without fail. All entries must be supplied in a ZIP file equal to or less than 250 KB in size. All source code, makefiles, documentation, and references to additional libraries used must be supplied in the ZIP file.
You can assume that everyone will have a copy of Allegro (standard installation) installed. You do not need to supply one. It is okay to use a more recent version of Allegro, but if someone is unable to compile your game because of that, it's your fault. You should consider uploading binaries for people who have problems compiling the source onto your own website. I will be checking that the binary and source match up, so adding enhancements to the 'competition binary' is not permitted..
If source code is reused from legal sources (your own, GPLed, public domain) you should declare this and what changes have been made, so that your work can be assessed for the voting.
People should keep a informative and interesting account of their development through the competition. This can be sent after the competition for those people with no Internet access over the weekend. This does not affect your space requirement.
A web-based "blog" update page will be available. This will allow spectators to see what is going on :-)
You can make use of all information sources, mailing lists as you see fit. This is not an exam! :-)


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BAUKNECHT PCT39 Service Manual
Product Reviews
There are currently no product reviews.
Reviews of our other manuals
Anton Kuyumdzhiev - 2007-04-06
Excellent copy of the manual and fast delivery, a great job thanks.
Antonio Balseiro - 2007-04-10
A really high quality document. Much better than expected. Perfect deal. Thanks.
Fred Hofstede - 2007-05-10
I ordered and received about 10 manuals now. All very good readable, even as the original when printed. And found every one I searched for. Verry satisfied and I can advise anyone to use Manuals in PDF as their source for good manuals of all kind Fred Hofstede The Netherlands
Fred Hofstede - 2007-05-19
Spledndid manual, already bought 17 manuals here and all in very good condition, even the images. I recommend Manuals in PDF to everyone.
arnold tempelmans plat - 2007-05-30
All manuals I ordered are first class. Only be aware when manuals are offered as Vol1 and Vol2 and as singles. Yoy do not know what you get.
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We provide manuals to wide range of devices, including:
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stan snieg - Ontario
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Stan Snieg is currently living in Ontario, and is interested in Consulting.
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Fuel Filter Repair Cost on 2001 Mercury Gran MArquis
2 Answers
The filter cost is $18 a service faculty will charge a total of approx $115. THIS does not mean this is the cause of the "stopped while running"
Find a back yard mech at your local bar.40 bucks done
Your Answer
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s Engine Noise Test Cell (ENTC) allows an engine to be run in a semi anechoic environment under the same conditions (speed, load, temperatures) as it would experience in a vehicle. We also boast of our exceptional range of interior doors starting from oak internal doors and glazed doors to folding sliding doors. I really enjoy working on illustration, comics and animation and buzzing between different projects for all three.
This blond babe is gorgeous and looks very alluring in her golden curly hair that is flowing down on her right shoulders and her neon green dress. Placement of an advert does not constitute endorsement although I will not accept advertisements from organisations that provide services contrary to my ethical standpoint and beliefs. Show Dates Tue 11th Oct: 09:30 17:00 Wed 12th Oct: 09:30 17:00 Thu 13th Oct: 09:30 16:00 Useful Links Book your stand Exhibitors Login Contact Venue & Travel Social Media Facebook Twitter Google + LinkedIn Youtube How to contact us +44 (0)20 8271 2155 Helitech®.
Replacement items will be sent out to the buyer once the original products are returned and inspected, a similar item or one agreed upon between the buyer and seller will be sent out in return. Security We may need to do some additional checks, including calling you back to ask security questions. ??The grand jury's vote not to bring an indictment in Garner's death – along with a similar outcome to the August police shooting of unarmed black teen Michael Brown in Ferguson, Missouri – has brought national unrest, with demonstrations in cities such as Washington, D.


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Hot Air Fried Polenta Rounds
Jo Zimny
Featured Pinch Tips Video
- 1-18 oz. pkg
- ancient-harvest pre-cooked polenta roll
- 1 Tbsp
- olive oil, extra virgin
1Open the Polenta Roll. With a sharp knife, cut the Polenta in 1/2 inch thick slices. Brush both sides of the slices with oil.
2Spray the basket of the hot air fryer with oil. Heat the air fryer for about 5 minutes at 400'F.
3Place the Polenta slices into the preheated air fryer and cook for 25 minutes. Then flip the slices and cook for another 5-10 minutes.
4Remove the slices and Enjoy!


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Painter tours Chicago
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Orbitz Worldwide, Inc., an online travel company, recently announced that it has reached an agreement with Avis Budget Group, Inc. to distribute Avis and Budget Rent A Car inventory, including hybrid vehicles, through its Orbitz and CheapTickets sites.
In April 2007, Orbitz launched eco.orbitz.com, a microsite that enables its customers to book more environmentally friendly travel. The company also formed a partnership with Carbonfund.org, enabling customers to offset their carbon footprint related to their travels.
The agreement provides Orbitz Worldwide customers with access to rental cars at Avis and Budget corporate-owned and select licensee locations in the U.S. and Canada. This access includes the Toyota Prius, the Nissan Altima and, available by year-end, the Ford Escape hybrid.
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As new forms of mobility take flight, the borders with traditional car rental are starting to erode.
Amazon just delivered its first package by drone in England. Will U.S. delivery fleets be able to take advantage soon?
With fewer drivers and substantially longer fleet lifecycles, fleet managers will pivot to new job functions.


text: Veronika Veronika is 30 years old female from Plzen, Czech Republic. She is interested in meet new people. 30, female, Plzen Likes: 519x Facebook profile Who s/he liked? Send message Favorite May she/he ask you for a date? Never Maybe Sure Relationship: ShowSexuality: ShowHeight: ShowWeight: ShowBody type: ShowEye color: ShowHair color: ShowLiving: ShowKids: ShowSmoking: ShowDrinking: Show Report photo Sign up to see all photos Facebook Twitter Google+ Pinterest Similar users Raina 29, Portland 268 Sharon 27, Chicago 297 PS3 Becca 32, Los Angeles online 383 Various Jessica 27, New York online 346 Salads Shqipe T. 32, Ridgewood 292 Joann 33, Anchorage 169 Amy 29, Sacramento 165 Firlin 30, San Mateo 186 lakesha 28, Williamsport 573 justask


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Greers Ferry, AR Energy
Greers Ferry Electricity Rates
^ Greers Ferry, AR electricity rates recently averaged 9.85¢/kWh -- an estimated 21 percent less than the average national rate. [More]
Greers Ferry Solar Energy
^ Solar energy output in Greers Ferry, AR is an estimated 35 percent higher than average solar energy in a low-output area of the nation. [More]
Greers Ferry Natural Gas Rates
^ Greers Ferry, AR natural gas rates recently averaged 18.42$/Mcf -- an estimated 34 percent more than the national average rate. [More]