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Kim Jong-un Is a Shoo-In for Time's Most Influential Person of the Year Thanks to 4chan
You'd think that after 4chan founder Christopher "moot" Poole was voted 2009's Most Influential Person in the World thanks in overwhelming part to a flood of 4chan users looking to game the system, Time magazine would quit handing the decision to Internet users.
But, happily, you'd be wrong.
Heads up, China: This year, 4chan is once again looking to take advantage of Time's lax approach toward bestowing hyperbolic titles by manipulating the survey to crown North Korean dictator Kim Jong-un 2012's most influential person.
The Glorious Leader's vote tally currently stands at an insurmountable 3,045,783 and climbing. Compare that with Egyptian president Mohamed Morsi, who is angling for first runner-up with just under 400,000 votes at the time of writing.
Then again, that too might be the work of /b/tards, who just launched phase two of the operation, which involves strategically voting for certain candidates so that the their names spell out "KJU GAS CHAMBERS."
[image via AP]


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John Crespi, Picturing the Purge: Chinese Cartoon Imagery from the 1930s to the 1950s, Part Four
MIT Visualizing Cultures
Picturing the Purge: Chinese Cartoon Imagery from the 1930s to the 1950s
Presented by John Crespi, Luce Associate Professor of Chinese; Director of Asian Studies, Colgate University
Visualizing Cultures was launched at MIT in 2002 to explore the potential of the Web for developing innovative image-driven scholarship and learning. The VC mission is to use new technology and hitherto inaccessible visual materials to reconstruct the past as people of the time visualized the world (or imagined it to be).
Visualizing Cultures Lectures
Help improve our information email MIT Video

subset: commoncrawl


text:
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A fabulous bungalow residence occupying a generous and mature site, on the edge of fintona village, very convenient to many local amenities including fintona golf club.
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text:
Silk Jersey Fabric
Sleek, soft and stretchy, silk jersey is a light to medium weight of a premium quality as it boasts flexibility, impeccable sheen, ideal strength, and absorbency that adds up to create a fabric that is as fluid as running water. Ideal for skin-contact garments as this jersey wicks away moisture while remaining breathable, perfect for tight skirts and form-fitting shirts. Wrinkle resistant and slinky, silk jersey travels well.
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text:
Insulin and Diabetes ManagementGlucose (Sugar) Monitoring
Monitoring glucose (sugar) levels in urine or blood has always been part of the insulin users' daily routine. The first home tests involved boiling urine over an alcohol burner and observing color change after adding a chemical reagent. Later kits replaced the alcohol and liquid chemicals with reactive tablets which required no exterior heat source. By the 1960s color changing testing strips were developed which reacted directly with urine.
Today, most testing is done using blood glucose, rather than urine glucose. Blood glucose testing was not possible outside of the laboratory until the 1970s when the first test strips and digital blood glucose monitoring devices became available for home use.
|Examination of Urine for Sugar with the Urine Sugar Test Case, Sheftel, from Diabetes Mellitus by Eli Lilly and Company. 1942.
"Insulin and Diabetes Management - Glucose (Sugar) Monitoring" showing 11 items.
Page 2 of 2
Ultra-Fine II Lancets
- Location
- Currently not on view
- date made
- ca 1997
- maker
- Becton, Dickinson and Company
- ID Number
- 2001.0113.2
- catalog number
- 2001.0113.2
- accession number
- 2001.0113
- Data Source
- National Museum of American History, Kenneth E. Behring Center


text:
Inside the Story, a review
The long wait is finally over. Today, Adam Westbrook's* ebook Inside The Story has been released and what a treat it is! The ebook Inside the Story: a masterclass in digital storytelling from the people who do it best is a collection of 20+ articles by several award winning and innovative storytellers, from all ranges of digital … Continue reading


text:
Macrium Reflect  5.1.5439
* Add Recovery Boot Menu Problem
- The 'Pre-Built WIM' boot menu option could fail to be created. This has been resolved.
* UEFI 'Backup Windows' option
- The 'Backup' > 'Backup Windows' menu option could exclude the automatic selection of the EFI system partition on UEFI booting systems. This has been resolved.
* GPT restore/cloning
- A program crash could occur if the target disk was encrypted prior to the restore/clone operation. This has been resolved.


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EU imposes sanctions after a truce collapses and bitter fighting resumes.
KIEV, Ukraine — A struggling leader in an ex-Soviet republic appealed to Russia for help battling extremists as the West called for calm so a peaceful resolution could be found.
The year was 2008, and the republic was Georgia. Now as then, Vladimir Putin lashed out at the United States and Europe for seeking to turn a former republic toward the West in the guise of a negotiated settlement.
In the end, Russia invaded the South Ossetia region of Georgia, and its tanks rolled all the way to the outskirts of the capital of Tbilisi until they halted. Russia's military is still in the country.
Some analysts say a similar scenario may be playing out in Ukraine, where as many as 100 anti-government protesters were killed Thursday in clashes with police in the service of a president who has been appealing to Moscow for help while Western diplomats pressure him to sit down and talk.
"When the music stops," does Ukraine want to be "on the losing side" with President Viktor Yanukovych's crumbling police state backed by Russia's muscle, "or on the side of political compromise and engagement with Europe?" asked Damon Wilson, who served as White House director of European affairs under President George W. Bush.
STORY: Fierce battles between police, protesters in Kiev
STORY: Hagel not able to engage Ukrainian counterpart
VOICES: Where are the diplomats to help Latin America?
STORY: Ukraine's army will likely balk if ordered into streets
A deal was struck Friday between Yanukovych and opposition party members to share power and hold early elections, a move many hoped would calm the situation. Many protesters said Friday they also insist the president resign, a demand that could renew tensions and violence.
After a brief truce collapsed Thursday, the European Union voted to impose sanctions on Ukraine's leaders responsible for the violence. The sanctions, unanimously approved by EU foreign ministers at an emergency meeting, include a travel ban to the 28-nation bloc and the freezing of assets held in EU countries.
The White House had condemned both sides for escalating violence and asked them to calm the situation. But in a telephone call Thursday with Yanukovych, Vice President Biden called upon the Ukraine president to immediately pull back all security forces, the White House said. Biden stressed that the United States "supports an independent, democratic Ukraine that pursues the future its people choose," the White House said in a statement.
And so embattled Ukraine finds itself caught squarely between the West, which has been wooing it, and Putin, who is clearly determined not to let the republic slip away.
Police and protesters, who are seeking a European-style democracy, continued to clash into the evening in Kiev. Protesters threw rocks and firebombs at lines of police after government snipers shot into crowds of protesters.
Ukrainian television showed scenes of protesters being gunned down and others lying on the street as comrades rushed up to pull them to safety, carrying them on planks of wood. Oleh Musiy, head of medical service, said the number of dead is "from 70 to 100 protesters."
The Ukraine Interior ministry says 67 police were captured by protesters. Video footage on Ukrainian television showed protesters leading captured police officers around a protest camp in central Kiev.
A 25-year-old woman speaks for Ukrainian protesters in a viral video, where she asks viewers to help build freedom in her country by sharing the video. Shannon Rae Green
The confrontation is not limited to the capital. In the West Ukraine, near Poland, protesters have taken over government buildings in Jhmelnytskyi, Ivano-Frankivsk, Uzhhorod and Ternopil, according to Ukrainian media. In Lvov, protesters have replaced the authorities with their own created council.
In Russian-speaking East Ukraine, attacks on both pro-government and opposition party buildings have taken place over the past few days. The offices of opposition leader Viktor Klitschko's Udar Party were attacked in the cities of Dnepropetrovsk and Krivoy Rog.
The split between East and West is a remnant of centuries of warfare, in which European powers and ethnic Cossacks and Slavs fought for control of the fertile land along the Black Sea. Czarist Russia asserted control in the east, and during World War II the Soviets reunited Ukraine with its Western provinces, then under Polish domination.
When the Soviet Union dissolved in 1991, Ukraine declared independence, along with many other former Soviet republics that had been forced to live under Communist rule.
But Russian President Putin has never accepted Ukraine's sovereignty or that of any of the republics. He has described their loss as "the greatest geopolitical catastrophe of the century" and has said that among his top priorities for his latest term is the reintegration of former Soviet republics through tighter economic links, political ties and security pacts.
He has said Moscow seeks a new Eurasian Union to rival the European Union and NATO, the U.S.-European military alliance.
"What we've got in Putin is a man with a strategic vision and an autocratic mentality," John Bolton, former U.S. ambassador to the United Nations under George W. Bush, said recently.
In January, Putin stepped in to offer Yanukovych a foreign aid package of $15 billion in cash and below-market-rate natural gas to aid Ukraine's economy, which has been ailing. It came with a string attached: Ukraine needed to reject an economic pact with the EU that had been in the works for years.
Russian Deputy Prime Minister Dmitry Rogozin threatened to cancel all joint space and defense industry projects if Ukraine made a move toward the European Union.
"Russia has never not intervened in Ukraine, that's the whole issue," said Ben Tonra, head of the School of Politics and International Relations at University College Dublin in Ireland. "President Putin has actively intervened in Ukrainian politics since the separation of Ukraine."
"There is an imbalance in terms of what tools the EU and Russia have in their disposal, and clearly the Russian Federation, as we have seen in Georgia, is willing to use military force as it deems necessary."
But now, amid the violence, European heads of state and foreign ministers are trying to pull Yanukovych in their direction with sticks of their own.
The EU said the sanctions it imposed Thursday will target "those responsible for human rights violations, violence and use of excessive force" in Ukraine, the EU said in a statement.
The U.S. State Department banned travel to the USA for 20 Ukrainian officials implicated in the violence and said it will consider joining European leaders in freezing bank accounts. It called on both sides to stop the violence.
"We are outraged by the images of Ukrainian security forces firing automatic weapons on their own people," White House press secretary Jay Carney said. "We urge President Yanukovych to immediately withdraw his security forces from downtown Kiev and to respect the right of peaceful protest, and we urge protesters to express themselves peacefully. "
Wilson, the former White House European affairs director, said such sanctions may be able "to avert civil war in the coming days and to salvage the prospect of a European future for Ukraine."
Many of the leaders of the ruling party have substantial wealth and investments in Europe and may be swayed by measures to keep them from their money.
But Bolton dismissed the sanctions as trivial. "If that's what people think motivate the hard men of power in control of the Ukrainian government, they're sadly mistaken," he said. "They love to stroll the European boulevards, but when it's that or maintaining their power in the Ukraine, this is worse than nothing."
The USA should make clear to the Ukrainian opposition and everyone in the country "that doesn't want to be brought into the Russian orbit" that the USA will push for Ukrainian membership in NATO, because "NATO provides security," Bolton said.
Russia is already talking like the uprising in Ukraine is a major security threat to the region that must be tamped down. Its Foreign Ministry on Thursday described the violence as an attempted coup and even used the phrase "brown revolution," an allusion to the Nazi rise to power in Germany in 1933.
The ministry said Russia would use "all our influence to restore peace and calm."
Sanctions are a "painful stereotype" that will only make things worse, says Alexander Konovalov, president of the Moscow-based Institute of Strategic Assessment.
"Sanctions against Moscow won't sway it, nor will sanctions against Ukraine. You need to stop the bloodshed, not issue sanctions," he said. "So they'll ban a few officials from entering the United States – so what? What is that going to change?"
Wilson said sanctions have helped influence government officials and politicians in the Ukraine and other former Soviet republics in the past, especially those who own homes and business assets in Europe. In Belarus in 2006, the USA used targeted sanctions against officials involved in trials against political dissidents, until the dissidents were released, Wilson said.
Fyodor Lukyanov, editor of the Russia in Global Affairs journal and chairman of the Council on Foreign and Defense Policy, says Putin is unlikely to take significant action to do anything about Ukraine.
"Russia is not going to openly get involved. It does not want to get pulled into this conflict because there are no good exits," Lukyanov said.
But what is worrying, Konovalov said, was that politicians in Russia, in their public rhetoric, were urging Yanukovych to act more decisively. On Thursday, Russian Prime Minister Dmitry Medvedev drew criticism on Twitter when he called on Yanukovych to not let opponents walk over him "like a doormat."
Some worry that the Crimea is where Putin could find a pretext to invade like he did in Georgia. A peninsula on the northern coast of the Black Sea, Crimea is where Russia's Black Sea Fleet is stationed. Ethnic Russians make up more than half of the population.
"Everyone knows that Russia is going to send troops to Ukraine – we have known it for a long time now," said Kateryna Chorna of Kiev, who has regularly taken part in the anti-government protests.
The protesters insist they will not give in.
"This is not the end of it — it's just beginning," said Oleh Hrynyshevskiy, a surgeon, as he helped wounded protesters in a first-aid post in the hall of a post office on Independence Square.
Activists rebuilding barricades in Independence Square expressed optimism despite the violence. Others said the broken truce made it clear Yanukovych is playing for time and has no intention of finding a peaceful solution.
But some members of the government were siding with the protesters. Kiev Mayor Volodymyr Makeyenko resigned from Yanukovych's ruling party along with 12 other deputies who urged law enforcement to refrain from violence.
"I think today will be a turning point for our revolution," said Igor Zhdanov, a protester in Kiev. "They started to surrender; we threw them back."
Contributing: Luigi Serenelli, Jennifer Collins in Berlin; Tom Vanden Brook in Washington


text:
Titanic Clearing Southampton, SI Negative #34,460
Since the ocean liner Titanic sank on its maiden or first voyage, there are very few original pictures of the ship in existence. Most of the photographs that do exist were taken in the Harland and Wolff Shipyard in Belfast, Ireland, while the ship was under construction. There is one that shows the new ocean liner entering the port of Southampton, England with the help of tugboats, several weeks before the ship was to take on passengers. There are also a few taken of the huge new ship as it cleared that port on its maiden voyage on 10 April 1912 with a full load of passengers. The Smithsonian owns one of the last of these photographs, showing the starboard or right side of the ship against the wharf. The wave at the bow of the vessel indicates that it is already picking up speed, as it readies for the open ocean.
Carpathia, the ship that rescued the Titanic survivors
Both the British and the Americans held formal inquiries and hearings on the Titanic loss. The investigations revealed that although several vessels heard Titanic's distress call and one was closer even than Carpathia when the call went out, only Carpathia responded in time to rescue survivors. As a result, Carpathia saved more than 700 Titanic passengers. The ships that returned to the area of the wreck site later only found bodies and debris from the Titanic that had floated up from the depths.
"Titanic - Introduction" showing 1 items.
Titanic Gold Medal
- Description
- Founded in 1904 by wealthy financier Andrew Carnegie in Pittsburgh, Pennsylvania, the Carnegie Hero Fund Commission (CHFC) exists to honor acts of individual civilian heroism in the United States and Canada. It is still active today; recipients include both the living, the dead, and persons directly affected by the loss of a heroic relative.
- The emotional impact on the general public of the April 1912 loss of the ocean liner Titanic was astonishing, and the continually updated story lasted for months in the contemporary newspapers. In Pittsburgh, Pennsylvania, the Commission felt inspired to honor all the heroes who had risked their lives in the rescue of the 700 passengers, so at their April 26, 1912 meeting they authorized a nine-oz. 22-k gold medal to be struck, mounted in an elaborate bronze base, inscribed and presented to the Smithsonian. The Smithsonian accepted the gift and displayed it before adding it to the National Numismatics Collection in the National Museum of American History.
- Location
- Currently not on view
- date made
- ca 1912
- maker
- Flanagan, John
- ID Number
- accession number
- 54893
- catalog number
- 13650
- Data Source
- National Museum of American History, Kenneth E. Behring Center


text:
Oh, I also do feel like spending the 75 bucks for a 5000mah battery either. Or at least I haven't found anything lower priced. Aside from venom, what are some reputable battery brands?
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Oh, I also do feel like spending the 75 bucks for a 5000mah battery either. Or at least I haven't found anything lower priced. Aside from venom, what are some reputable battery brands?
BIAS batteries from atomikrc.com are pretty good for the buck; 50 bucks gets you 50C and 5000 MAh. If you don't want the Atomik path, A Main Hobbies' EcoPower batteries are cheap and great for buggies.
Atomik/Venom Team Driver
Ok, good to know, how are the onyx brand? Both lhs in town carry them. Cheap at least for the nimh sets, unsure on Lipo if they carry them either.
Onyx LiPOs aren't the greatest, but could get the job done.
Atomik/Venom Team Driver
I paid 15 for the onyx nimh that I have, but not sure what the lipos cost. Their dual chargers are expensive, 130 in town though which is why I checked for reviews on others and went with the thunder ac6 for 45. Ascend and one other brand they had, ok for price, but wanted to see reviews.
I'm coming to the conclusion that this has a lot of parts from the truck version of the rc10 after looking at other ones. Not sure if it was done in convenience or a conversion.
Probably a conversion? The tires on the back remind me vaguely of stadium truck tires. Actually, any LiPO should work good for backyard bashing and having fun. Ascend is pretty decent and so is Onyx. Really up to you.
Atomik/Venom Team Driver
It came with a set of body posts for a truck body which I've seen tagged as a 90's version rc10** if I remember right, font shock mount tower also won't let the body pass through, and all the arms are wider, about 2.5 to 3 inches as a set of original rears came with it.
Dbl post, delete.
Mike Lawrence
It\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'s nice to be important, but it\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'\'s more important to be nice
That was before I picked up, after talking to the guy I got it from and seeing how old he is has to put it to the earliest years it was made. With the longer arms and a good motor, I think it could hold its own on the track. I haven't done any racing yet, but do plan to, just bash for now. Seems every time I take my volcano out lately though I break it....
I'd be all over buying that car for $50 I'd even happily drop $100 on it, mainly due to the memories I have as a teenager with my original rc10. I still remember staying up till 4am putting my first one together and how excited I was upgrading to the stealth tranny.
Yeah, I'm gonna pick up the gold front, retain the rpm bumper that's on it, and I'm still trying to figure out the servo linkage, might wind up picking up new steering horns and linkage.
Last edited by greentornado; 07-31-2014 at 02:29 PM. Reason: phone is ....
How can i tell what Mah and voltage to set these batteries at to cycle them a few times? 2 are nicd, and Im unsure on the other one, one is 2400 Mah, All are 6 cell.
SMC Nicd 2400Mah
PTI Nicd
Cycle them with charge at 2 amps, and discharge at .5 amps.
Atomik/Venom Team Driver
I'm trying to look close at the details in the photo but what I believe you have is a mix of different Associated vehicles. It's hard to tell from a photo but it think the car may have started out as a buggy possibly. Could have been a truck since it appears to have the stealth transmission but I think someone attached suspension from a RC10T2. The front end is not from an RC10T, at least not all of it, it's likely a T2 shock tower. Although the black nose piece appears to be RC10. The shock setup isn't correct for that time period, probably from a T2 or T3.
I couldn't see all of it but it looks like you don't have the 6-gear transmission which uses 32P gears and an external ball diff built into the spur. The give away is the spur gear on your car without the diff plates on both sides. I saw the half tranny case in the parts which is from the earlier Stealth gearbox. The 6-gear is about the same size but it has squared edges and isn't nearly as contoured.
Being a gold chassis and if it was originally a 10T then it dates to about '91. But the black nose was found on the Championship Editions, I belive the Team car and definitely the Worlds car from '94. Unless this example is painted black, I can't tell. I see original RC10 arms mixed in the parts but they look too short for the 10T. I have a RC10 Graphite from '89 or so which had those arms and the 6-gear tranny. All the suspension parts were basically the same except that it was all bolted to a flat carbon fiber pan.
Here are some closer up shots. 3 gear stealth tranny, came with the original rear arms, original front/rear arm supports, original gold front shocks (broken plastic and scratched up really bad). In the last pic, left side between the arm and the nose brace, im not sure if that is the front body mount/upper arm linkage for buggy or not.
Those are good photos. I'm going to say that this is a 10T but, at some point it was missing parts or a previous owner wanted to do some upgrades and installed T2 arms, front shock tower, shocks and mounts. The steering knuckles appear to be correct for a 10T, however, they would have originally been white. Associated did offer optional black plastic parts for the RC10s and RPM made a few pieces too. I've switch most of my car to black plastic with RPM rear arms specifically for RC10s. Anyhow, that piece in the last photo is a "upper deck" or "chassis brace" from another vehicle. Maybe the T2 / B2, I don't recall what they look like. But, it won't fit onto this truck. One later generations, this is what replaced the aluminum tubes when they switched from aluminum pans to composite chassis'. I also see the original style front arm / shock tower mounts in the center. Those are RC10 rear arms but I want to say those are buggy arms, truck arms might have been a little longer. Hard to say from a photo.
It's a customized RC10T. Unique but whoever did the customization apparently stuck with T2 parts which isn't necessarily a bad thing. It hurts collector value but, it makes the truck drive more like a modern vehicle while retaining some vintage appearance. Cool!
Rear arms, front/Rear arm holders, all 4 gold shocks, both battery trays and rear bulkhead I know are rc10 from looking through associated parts. The rc10 arms in the last pic are really stained and brittle, where the rest of the arms still have the flexibility that they should have. My goal at this point is to return it back to the buggy form. Gold front, black arms, new rpm bumper (added versatility since to rc10 didn't have one from what I've seen, just the truck version), new motor/esc and replace the body that is in horrible condition. I'll probably buy new gold shock pieces too. It has the rc10 gold and black shocks and rc10 shocks, all full sets, just most of them are broken. So I figure 80 on buggy parts, 15 on body, 40ish on motor and esc, 15 to 20 on a screw set and it should pretty much be back to a good running vintage style buggy again. I'll probably have it for a year or 2 then look into selling it for another project, with my timeframe right now and working more on the tornado and m8, I'm thinking 2 months or so and I'll have this pretty much done.


text:
Daria Ullrichová
Stage director: Michal Dočekal
Sets: David Marek
Costumes: Kateřina Štefková
Music: Wolfgang Amadeus Mozart, Aleš Březina
Dramaturgy: Daria Ullrichová
The classic French comedy has not aged at all. On his wedding day, the servant Figaro defends his beloved Susanna against the amorous advances of Count Almaviva, who wants to apply the right to the first night. We are entertained by Figaro's adroitness and guile and applaud his courage to ask emphatically why there is a class of chosen ones reaping privilege and advantage. The play's social aspect has become acutely topical again after more than two centuries. The production is inspired not only by Beaumarchais's play but also Da Ponte's libretto and Mozart's divine music.
Drama in czech language.
Photo: Martin Špelda
Duration of the performance: 2 hours and 50 minutes, 1 intermission


text:
L.Joshua Frazier ?@JoshuaFrazier99 Does anyone else know about this a reliable source told me John Gruden is coming to be New head Coach at Arkansas??!!
There's no reason to get excited about any candidate until they're introducing their wife and kids and awkwardly calling the hogs.
Jeez, thanks for the warning to not go over there.
Pretty much any name that has come up, I have put them on the "not happening" list b/c that's exactly how it unfolded the last time.
you would think hogville would have learned from our last coaching search that JL is not going to let anyone in on this until it's officially announced, and is going to troll the ark media like a mother fricker.
Ah but they have that angle figured out this time around. They are now saying that all sources coming from the BAC are bogus (any candidate not named Gruden) but the Gruden rumors are legit because they are coming from the money that is putting the deal together for Long.
Problem solved. Now the story is completely believable again.
The only place you hear Gruden's name with Arkansas head coach is on HV.
I've been on the Gruden train ever since April. However, I would be JUST AS HAPPY with Pete Carroll as I would be with Gruden
I suspect he is talking about Tubberville. Tubberville's interest in the job is good only as a firewall against Long making a god awful decision. He should not be considered a first choice.
Well, if it ends up being a hire like Gruden or Carroll, I will be the first to send an apology letter to JL.
Remember JL does not hire coaches at the UofA. Or at least that's what I've noticed from the last 2 hires.
It wasn't "ties" to the state. It was about the "ties" that Gruden wore on MNF. It was "anthracite & red" last week....the plot thickens.
Okay. Y'all all reading way too much into this. Schaap meant Gruden. That came directly from Trey's mouth. I wouldn't put a ton of stock into it, and I certainly wouldn't get my hopes up, yet. But that tweet was absolutely about Gruden.
Gruden knows Jerry...Jerry has ties to the program and the state.
I can't get any of them to answer a question right now and that might be a good thing. They're usually very quick to answer when there's nothing to a subject.
I can contribute to this thread. A lot of the big time money boosters are wanting an announcement ASAP. One of the biggest boosters said the money is in place for Gruden to make 1.5-2 million dollars more a year than Bobby made. I don't know what that amount is but I'm sure you guys can tell me. He also said that the deal is in place for Gruden to sign which contradicts what Schaap said of it already being a done deal. However, Schaap is reliable and has a good source on this.
I'll say this. There are a lot of people saying Gruden and I feel like they have no connection to each other to be making it up.
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Stare long enough and you will find yourself moonstruck, so it may be best to allow this photo a few seconds only.
It is like reading too many books, or taking that one drink too many—moderation may prove hard to come by after a while.
There is something about the moon that magnet-like pulls us into the night air, and, whether we choose to admit it or not, some of us find ourselves victims of enchantment before the screen door slams. We are lost in what is both alien and familiar.
Springhouse,P. O. Box 8,Herod, IL 62947


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Dry yeast
(→External Links)
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[[Image:Nott_sachet-1-.jpg|thumb|Dried Yeast]]
[[Image:Nott_sachet-1-.jpg|thumb|Dried Yeast]]
'''Dry yeast strains.'''
'''Dry yeast strains.'''
YS550 Muntons Gold Ale Yeast - One of the best English Ale yeasts. Use 2 packs in 5 gal
YS550 Muntons Gold Ale Yeast - One of the best English Ale yeasts. Use 2 packs in 5 gal
Revision as of 21:35, 13 February 2007
What is dried yeast
DRIED YEAST, Saccharomyces cerevisiae
Dry yeast strains.
YS550 Muntons Gold Ale Yeast - One of the best English Ale yeasts. Use 2 packs in 5 gal
YS530 Doric Canadian Ale Yeast - Used in our Kits, this is a great fast-starting yeast strain
SAF Ale S-04 Yeast - Whitbread strain famous for its excellent attenuation and flavorful profile.
SAF Ale S-33 Yeast - Clean, quick general purpose yeast. Compare to Edme.
SAF Ale T-58 Yeast - Estery, spicy & peppery flavors in wheats and speciality ales, very solid sediment.
- Danstar Nottingham Ale Yeast - English strain noted for producing clear beers
- The Nottingham strain was selected for its highly flocculant (precipitating) and relatively full attenuation (transforming sugar into alcohol) properties. It produces low concentrations of fruity and estery aromas and has been described as neutral for an ale yeast, allowing the full natural flavor of malt to develop. Good tolerance to low fermentation temperatures, 14°C (57°F), allow this strain to brew lager-style beer. Recommended 14° to 21°C (57° to 70°F) fermentation temperature range.
- Windsor English Ale Yeast - This yeast strain is traditional for Brown Ales
- Danstar Windsor ale yeast originates in England. This yeast produces a beer which is estery to both palate and nose with a slight fresh yeasty flavor. These are usually described as full-bodied, fruity English ales. Depending on the substrate, the Windsor demonstrates moderate attenuation which will leave a relatively high gravity (density). Recommended 17° to 21°C (64° to 70°F) fermentation temperature range.
SAF ALE US-56 American Ale (Chico Strain) - Finally! The most famous ale yeast strain found across America, now available as a convenient and economical ready-to-pitch dry yeast. Produces well balanced beers with low diacetyl and a very clean, crisp end palate.
Brewferm Lager Yeast - Very clean lager yeast, delivering consistent neutral fermentation with little or no sulphur components and other undesirable by-products.
SAF Lager S-23 Yeast - A new high-quality commercial brewery Lager yeast
External Links
Danstar Nottingham fact sheet
Danstar Windsor fact sheet
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Want to be clad in head-to-toe denim? Denim Therapy has become the expert in denim repairs and making sure our clients can keep wearing their favorite blues for as long as they both shall live. That's why we've teamed up with Toms, an equally environmentally conscious company, to offer a few lucky denim heads the chance to win a free pair of Toms Denim Bimini Stitchouts. Enter here to win and you could be strutting your stuff in style this Fall. The terms are simple:
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—Sarah Greene
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At Staten Island University Hospital, our physicians have earned a reputation
for excellence and innovation in education,
research and patient care. Our doctors are
renowned in their fields and lead
exceptional teams of nurses, technicians,
clinicians and support staff that deliver
advanced, compassionate care
to their patients.
Learn More
Staten Island University Hospital provides complete medical
and surgical services in inpatient and outpatient
settings at both our North and South site locations.
Read on to learn more about the full range of
services offered at SIUH, including
locations, contact information
and descriptions of programs.
At Staten Island University Hospital, we strive to provide
the best health care for our patients. We also seek
to make their stay as comfortable as possible.
That's why we provide many helpful
resources for patients and visitors
to help them navigate our
hospital with ease.
Would you like to visit someone who is a patient at Staten Island University Hospital? If so, we welcome you. Family and friends
can play a key role in the healing process
for patients. You can serve as a
companion, advocate and be a
participant in their care.
Staten Island University Hospital is part of the Staten Island community. In addition to providing state-of-the-art healthcare services, we educate our
neighbors by sponsoring
health-related community
events and support groups.
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Providing quality health care for our patients is our greatest priority.
In order to do this, our hospital family is committed
to the education of our medical caregivers.
We provide accredited training and
educational programs for physicians, nurses,
allied health professionals and technicians.
Kathryn Carse/Staten Island Advance
STATEN ISLAND, N.Y. -- We're all going to die sometime, but if you're a man, you want to die from something else beside prostate or testicular cancer.
That's the way Michael O'Brien looks at it.
Not because it is any worse than any other way to die, but because it is preventable. If you catch it early, and get treated, the chances are very good it won't kill you.
So he is growing a mustache.
The 30-something lawyer has joined men around the world who are celebrating Movember — a mustache movement during November to "change the face of men's health."
"It's a fun thing to do for a very serious underlying reason," said O'Brien, a Grymes Hill resident.
Why a mustache?
Basically, much of men's health problems comes from them not talking about them, not going to the doctors or seeking preventive tests for diseases such as testicular and prostate cancer. And forget about talking about mental health.
However, grow some facial hair, and everyone has something to say about it — from Mom to wife to strangers.
That is what a bunch of men in Australia found out in 2003 when they grew mustaches — known Down Under as a "mo." It sparked so many conversations that the next year they organized the effort so more men could harness all that attention for a good cause — to raise awareness and funds for men's cancers and mental health issues.
O'Brien, whose law practice O'Brien and Jacobs is located in St. George and Manhattan, joined the effort through the Staten Island Economic Development Corp. (SIEDC) where the Emerging Entrepreneurs Council (EEC) formed a team to support Island men — Mo Bros — and the women who care about them — Mo Sistas — to participate in the hairy movement to raise awareness and funds.
"With my own dad, my father-in-law, brothers-in-law, it helps me nudge them and say, 'Hey, get yourself checked out. We want you around a long time,' " said O'Brien, who joined some teammates at In the Mixx salon for a clean shave before whisker-growing.
The way to get "checked out" is a trip to the urologist, especially between the ages of 50 and 70, for a PSA and a DRE.
The PSA test is a blood screening that detects prostate specific antigen (PSA), a protein that is produced by the prostate gland. A high level can indicate the presence of cancer, but it is used with other diagnostic tools, in particular the DRE.
That's a digital rectal exam — the digit is the doctor's finger.
"No one is running in here dying to have a rectal exam," said Dr. Michael Savino, associated director of urology at Staten Island University Hospital.
Co-workers, brothers, cousins, uncles love to kid men about the procedure, so they come in with all kinds of preconceived notions, said Dr. Savino. But after the first one, the stories are laid to rest.
"It takes 10 seconds; 15 at the most. If it takes longer, you're in the wrong place," said Dr. Savino, who doesn't seem to need a mustache to lighten up the approach to deadly issues. (He hasn't decided about growing a mustache. His sons are doing it, but his wife warns him he will look older.)
About one man in six will be diagnosed with prostate cancer in his lifetime. On Staten Island, it was the second highest form of cancer after breast cancer and followed by lung and colorectal cancers.
The problem with prostate cancer is there are no symptoms in the early stages, and if men feel fine, they don't go to the doctor.
"You want to feel OK when you come; then we have a chance of early detection," said Dr. Savino. On the other hand, if you are experiencing symptoms — urinating blood, feeling sluggish or weak, loss of appetite, losing weight — there is a chance you are in an advanced stage of prostate cancer.
Although testicular cancer, compared to other cancers, is rare, it is most common in males between 15 and 35. It also has easily detected symptoms and is highly treatable.
Men of any age who feel a small hard lump, with swelling or a change in the consistency of the testicle, should see a doctor, which according to Dr. Savino, they don't usually need to be persuaded to do.
"Men show up early when they detect anything different there. In the shower, or if a wife, girlfriend, boyfriend feels a mass — and they are in the office. They might wait a week or month to see if it goes away, but they show up," said Dr. Savino.
If found and treated early, the cure rate is about 95 percent.
Men often get a head start on a beard or mustache when they are on vacation or home with the flu, which avoids the prickly, "what's that on your lip?" stage.
During Movember, participants suffer through those early awkward days in public.
"I look like my father in the '80s," says Steven Grillo. "All I need is a Members Only jacket."
But for Grillo, it's all worth it. At 28, the need to be pro-active came home early — his father-in-law is being treated for an advanced stage of prostate cancer. A project manager with the SIEDC, Grillo embraced the comic relief of a mustache (even if it was at his own expense) and joined its Movember team.
With his wife poking fun on Facebook at that thing on his lip, family and friends can use his "goofy" look as an opening salvo to contact his father-in-law.
"Being treated for cancer can be a depressing, solitary thing. The mustache creates a conversation opener. If it's just talking about mustaches, that's great too, but it also gives him a chance to talk about his chemo and how he is feeling," said Grillo.
In support of his father-in-law, Grillo already has collected $1,125 for the SIEDC team.
With some three million Mo Bros and their supporting Mo Sistas from 21 countries around the world, the Movember Foundation redistributes funds to projects all over the world for research, education and treatment of men's cancers and mental health challenges.
What does mental health have in common with men's cancers? Mental health issues are common, avoided by men and effective treatments are available.
Getting help for a mental health issue can be straightforward, says Dr. Stephen Wakschal, a psychologist and director of Victory Behavioral Health in Willowbrook. A therapist helps you look at the underlying reason for depression or anxiety and comes up with a game plan.
"It's more like coaching," he said.
Wakschal says most depression and anxiety comes from a sense of loss during a time of transition — good or bad.
It can be confusing, he said, when you feel sad about happy events such as getting married, having a child or starting a new job. But these events bring a shift in responsibilities and changes in routines such as having less time hanging out.
And, Wakschal asks his patients to consider, when the transition is more difficult — leaving the parental home, taking care of an aging parent, having a special needs child, retiring, getting fired, losing a spouse or a friend — wouldn't it be strange if you felt great?
He encourages men to consider the intensity, duration and frequency of a feeling and if it is interfering with something in their life.
"If you are becoming isolated, not taking pleasure in things that you did, if it is interfering with something in your life," says Wakschal, you should speak to your doctor about seeing a mental health professional.
At Staten Island Radiation Oncology, where men's health is an everyday concern, Movember is being embraced as a light-hearted way to support the patients being treated for prostate and testicular cancer.
"It takes their mind off of what they are here for; you get a smile," said chief therapist Kirk Krickmier, who joined a Movember team with his co-workers.
Typically, he said, male patients respond to questions about how they feel with a brief, "Great" or "Perfect."
But a mustache can help loosen up the conversation and get the men to talk a little more about what they are going through.
The success of the American Cancer Society's Pink Ribbon campaign is hard to ignore, especially when the burly men of the NFL don pink accessories.
"Men's health hasn't gotten that type of exposure. If this helps get the conversation started, that's great," said Grillo.
And women who play an important role in the health of the men they care for are encouraged to become a Mo Sista to support the effort by talking it up in social media, raising funds and helping to organize social events.
In 2012, over 1.1 million Mo Bros and Mo Sistas around the world raised $147 million, with U.S. over 209,000 Mo's raising $21 million.
Register and find information about the movement and resources on men's health at Movember.com.
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- Unisex Outerwear
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About This Product
Adult size zip front water-resistant, hip-length nylon taffeta jacket is lined with lightweight brushed fleece for early morning warmth. Elastic waist and cuffs provide a snug yet forgiving fit. Attached hood w/ drawstring. 2 outer pockets, 1 inner pocket. Outer shell is 100% Nylon, inner liner is brushed polyester fleece. Imported. For Youth sizes XS-XL see style 53402.


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Santa Cruz discontinued a large number of its polyclonal products as a result of the USDA settlement that was made public May 19th 2016
Find CST Equivalent
To Purchase # 9800S
|40 assays
Find answers on our FAQs page.
PhosphoSitePlus® Resource
- Additional protein information
- Analytical tools
p44/42 MAP Kinase Assay Kit (Nonradioactive) #9800
p44/42 MAP Kinase-induced phosphorylation of Elk-1 was measured by immunoblotting with Phospho-Elk-1 (Ser 383)9186, mouse mAb. Lysate titrations were run by immunoprecipitating various amounts of Phospho-p44/42 from 3T3, PDGF lysates and 3T3, U0126 lysates. Phosphorylation of Elk-1 (Ser383) is clearly seen in the positive control lysate compared with the same amounts of the negative control.Learn more about how we get our images
Gallery: p44/42 MAP Kinase Assay Kit (Nonradioactive) #9800
- p44/42 MAP Kinase-induced phosphorylation of Elk-1 was measured by immunoblotting with Phospho-Elk-1 (Ser 383)9186, mouse mAb. Lysate titrations were run by immunoprecipitating various amounts of Phospho-p44/42 from 3T3, PDGF lysates and 3T3, U0126 lysates. Phosphorylation of Elk-1 (Ser383) is clearly seen in the positive control lysate compared with the same amounts of the negative control.
Western Blotting Protocol
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
- 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
- 10X Tris Buffered Saline (TBS): (#12498) To prepare 1 L 1X TBS: add 100 ml 10X to 900 ml dH2O, mix.
- 1X SDS Sample Buffer: Blue Loading Pack (#7722) or Red Loading Pack (#7723) Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer. Dilute to 1X with dH2O.
- 10X Tris-Glycine SDS Running Buffer: (#4050) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH2O, mix.
- 10X Tris-Glycine Transfer Buffer: (#12539) To prepare 1 L 1X Transfer Buffer: add 100 ml 10X Transfer Buffer to 200 ml methanol + 700 ml dH2O, mix.
- 10X Tris Buffered Saline with Tween® 20 (TBST): (#9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH2O, mix.
- Nonfat Dry Milk: (#9999).
- Blocking Buffer: 1X TBST with 5% w/v nonfat dry milk; for 150 ml, add 7.5 g nonfat dry milk to 150 ml 1X TBST and mix well.
- Wash Buffer: (#9997) 1X TBST.
- Bovine Serum Albumin (BSA): (#9998).
- Primary Antibody Dilution Buffer: 1X TBST with 5% BSA; for 20 ml, add 1.0 g BSA to 20 ml 1X TBST and mix well.
- Biotinylated Protein Ladder Detection Pack: (#7727).
- Prestained Protein Marker, Broad Range (11-190 kDa): (#13953).
- Blotting Membrane and Paper: (#12369) This protocol has been optimized for nitrocellulose membranes. Pore size 0.2 µm is generally recommended.
- Secondary Antibody Conjugated to HRP: Anti-mouse IgG, HRP-linked Antibody (#7076).
- Detection Reagent: SignalFire™ ECL Reagent (#6883).
B. Protein Blotting
A general protocol for sample preparation.
- Treat cells by adding fresh media containing regulator for desired time.
- Aspirate media from cultures; wash cells with 1X PBS; aspirate.
- Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice.
- Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).
- Heat a 20 µl sample to 95–100°C for 5 min; cool on ice.
- Microcentrifuge for 5 min.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#13953, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
- Electrotransfer to nitrocellulose membrane (#12369).
C. Membrane Blocking and Antibody Incubations
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
I. Membrane Blocking
- (Optional) After transfer, wash nitrocellulose membrane with 25 ml TBS for 5 min at room temperature.
- Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
II. Primary Antibody Incubation
- Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product datasheet) in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4°C.
- Wash three times for 5 min each with 15 ml of TBST.
- Incubate membrane with Anti-mouse IgG, HRP-linked Antibody (#7076 at 1:2000) and Anti-biotin, HRP-linked Antibody (#7075 at 1:1000–1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
- Proceed with detection (Section D).
D. Detection of Proteins
Directions for Use:
- Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST.
- Prepare 1X SignalFire™ ECL Reagent (#6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.
- Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Western Blot Reprobing Protocol
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
- Wash Buffer: Tris Buffered Saline with Tween® 20 (TBST-10X) (#9997)
- Stripping Buffer: To prepare 100 ml, mix 6.25 ml of 1M Tris-HCl pH 6.8, 10 ml of 20% SDS and 700 μl β-mercaptoethanol. Bring to 100 ml with deionized H20. Make buffer fresh just prior to use.
B. Protocol
- After film exposure, wash membrane four times for 5 min each in TBST. Best results are obtained if the membrane is not allowed to dry.
- Incubate membrane for 30 min at 50°C in stripping buffer (with slight agitation).
- Wash membrane six times for 5 min each in TBST.
- (Optional) To assure that the original signal is removed, wash membrane twice for 5 min each with 10 ml of TBST. Incubate membrane with LumiGLO® with gentle agitation for 1 min at room temperature. Drain membrane of excess developing solution. Do not let dry. Wrap in plastic wrap and expose to x-ray film.
- Wash membrane again four times for 5 min each in TBST.
- The membrane is now ready to reuse. Start detection at the "Membrane Blocking and Antibody Incubations" step in the Western Immunoblotting Protocol.
posted June 2005
revised June 2016
Immunoprecipitation for Analysis by Western Blotting
This protocol is intended for immunoprecipitation of native proteins for analysis by western immunoblot or kinase activity.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
- 20X Phosphate Buffered Saline (PBS): (#9808).
10X Cell Lysis Buffer: (#9803) 20 mM Tris (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM Sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 μg/ml Leupeptin
NOTE: CST recommends adding 1 mM PMSF (#8553) before use*.
- 3X SDS Sample Buffer: (#7722) 187.5 mM Tris-HCl (pH 6.8 at 25°C), 6% w/v SDS, 30% glycerol, 150 mM DTT, 0.03% w/v bromophenol blue
- 10X Kinase Buffer (for kinase assays): (#9802) To Prepare 1 ml of 1X kinase buffer, add 100 µl 10X kinase buffer to 900 µl dH2O, mix.
- ATP (10 mM) (for kinase assays): (#9804) To prepare 0.5 ml of ATP (200 µM), add 10 µl ATP (10 mM) to 490 µl 1X kinase buffer.
B. Preparing Cell Lysates
- Aspirate media. Treat cells by adding fresh media containing regulator for desired time.
- To harvest cells under nondenaturing conditions, remove media and rinse cells once with ice-cold PBS.
- Remove PBS and add 0.5 ml 1X ice-cold cell lysis buffer to each plate (10 cm) and incubate the plates on ice for 5 minutes.
- Scrape cells off the plates and transfer to microcentrifuge tubes. Keep on ice.
- Sonicate samples on ice three times for 5 seconds each.
- Microcentrifuge for 10 minutes at 4°C, 14,000 x g, and transfer the supernatant to a new tube. If necessary, lysate can be stored at –80°C.
C. Immunoprecipitation
- Take 200 μl cell lysate and add 10 μl of the immobilized antibody, incubate with rotation overnight at 4°C.
- Microcentrifuge for 30 seconds at 4°C. Wash pellet five times with 500 μl of 1X cell lysis buffer. Keep on ice during washes.
- Proceed to sample analysis by western blotting or kinase activity (section D).
D. Sample Analysis
Proceed to one of the following specific set of steps.
For Analysis by Western Immunoblotting
- Resuspend the pellet with 20 µl 3X SDS sample buffer. Vortex, then microcentrifuge for 30 sec at 14,000 x g.
- Heat the sample to 95–100°C for 2-5 min and microcentrifuge for 1 min at 14,000 x g.
- Load the sample (15–30 µl) on a 4–20% gel for SDS-PAGE.
- Analyze sample by western blot (see Western Immunoblotting Protocol).
NOTE: To minimize masking caused by denatured IgG heavy chains (~50 kDa), we recommend using Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb (#3677) or Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (#3678) (or HRP conjugate #5127). To minimize masking caused by denatured IgG light chains (~25 kDa), we recommend using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (#3678) (or HRP conjugate #5127).
For Analysis by Kinase Assay
- Wash pellet twice with 500 µl 1X kinase buffer. Keep on ice.
- Suspend pellet in 40 µl 1X kinase buffer supplemented with 200 µM ATP and appropriate substrate.
- Incubate for 30 min at 30°C.
- Terminate reaction with 20 µl 3X SDS sample buffer. Vortex, then microcentrifuge for 30 sec.
- Transfer supernatant containing phosphorylated substrate to another tube.
- Heat the sample to 95–100°C for 2–5 min and microcentrifuge for 1 min at 14,000 x g.
- Load the sample (15–30 µl) on SDS-PAGE (4–20%).
posted December 2007
|Product Includes
|Quantity (with Count)
|Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Sepharose® Bead Conjugate) 3510
|1 x 400 µl
|Phospho-Elk-1 (Ser383) (2B1) Mouse mAb 9186
|1 x 100 µl
|Elk-1 Fusion Protein 9184
|1 x 40 µl
|Kinase Buffer (10X) 9802
|1 x 15 ml
|Cell Lysis Buffer (10X) 9803
|1 x 15 ml
|ATP (10 mM) 9804
|1 x 50 µl
|Anti-mouse IgG, HRP-linked Antibody 7076
|1 x 100 µl
|Anti-biotin, HRP-linked Antibody 7075
|1 x 100 µl
|Biotinylated Protein Ladder Detection Pack 7727
|1 x 100 µl
|20X LumiGLO® Reagent and 20X Peroxide 7003
|1 x 5 ml each
Nonradioactive p44/42 MAP Kinase Assay Kit provides all the reagents necessary for measuring p44/42 MAP kinase activity in the cell. Immobilized Phospho-p44/42 MAPK (Thr202/Tyr204) mAb is used to immunoprecipitate active p44/42 MAP kinase from cell extracts, then an in vitro kinase assay is performed using Elk-1 protein as a substrate. Elk-1 phosphorylation is then detected by Western blotting using Phospho-Elk-1 (Ser383) Antibody.
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.
1. Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
2. Baccarini, M. (2005) FEBS Lett 579, 3271-7.
3. Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
4. Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
5. Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
6. Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
7. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
8. Marais, R. et al. (1993) Cell 73, 381-93.
9. Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
10. Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
Phuchareon, J. et al. (2015) Proc Natl Acad Sci U S A 112, E3855-63.
Russell, K.S. et al. (2000) Proc Natl Acad Sci U S A 97, 5930-5.
Jost, M. et al. (2001) . J. Biol. Chem. 276, 6320-6326.
Skapenko, A. et al. (2001) J Immunol 166, 4283-92.
Gui, Y. and Zheng, X.L. (2003) J Biol Chem 278, 53017-25.
Hsu, H.Y. et al. (2004) J Immunol 173, 5989-99.
Wong, C.H. et al. (2005) Endocrinology 146, 1192-204.
He, Z. et al. (2005) J Biol Chem 280, 2446-54.
Schlezinger, J.J. et al. (2006) Toxicol Sci 92, 433-44.
Jia, W. et al. (2006) Mol Cancer Res 4, 549-62.
Tan, J. et al. (2000) J Neurosci 20, 7587-94.
Zampieri, C.A. et al. (2007) J Virol 81, 1230-40.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.


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As the flagship sedan in the Acura lineup, the 2003 RL boasts impressive levels of power, handling and luxury, allowing it to compete easily with the Mercedes E320, BMW 530i, Audi 3.0 A6, and Lexus GS 300.
The RL's 3.5-liter V-6 engine is the biggest engine to have ever powered an Acura sedan. This potent powerplant generates 225 horsepower while at the same time, delivering impressive fuel efficiency and low tailpipe emissions. The RL is classified by the EPA as a Low Emission Vehicle (LEV).
A firm, enthusiast-oriented suspension enhances the 2003 RL's handling responsiveness and stability. Wide wheels and tires enhance cornering grip, while a speed-sensitive, power-steering system improves handling feel. For superb control in all driving situations, the RL is equipped with Acura's highly sophisticated Vehicle Stability Assist (VSA) system, which helps provide both traction control and stability assistance.
Noise, Vibration, and Harshness (NVH) are kept to minimum levels through extensive use of insulation in the engine compartment and under the dash.
A standard OnStar® system provides drivers with 24-hour voice access to a live OnStar advisor, who can provide emergency assistance. An available OnStar route guidance concierge and a Virtual Advisor feature allow the driver to obtain news updates and sports scores and even listen to e-mail using a hands-free, voice-activated Internet connection.
Outside, the 2003 RL gets a new taillight lens with a clear turn signal portion, redesigned alloy wheels and three new colors.
The 3.5 RL comes fully equipped. Standard features include leather seating surfaces, a power moonroof, an Acura/Bose Music System with six-disc CD changer, an Automatic Climate Control System, heated front seats and door mirrors, and the HomeLink® remote control system.
For 2003, the RL gets a new interior color, Slate, as well as three new wood trim colors Auburn Camphor, Golden Camphor and Black Camphor.
Also available is the DVD-based Acura Satellite-Linked Navigation System, which offers route guidance to almost any destination in the continental United States and information on more than 3.7 million points of interest.
The high-performance, technologically advanced 2003 Acura 3.5 RL provides:
- A potent, refined, four-valves-per-cylinder engine, delivering 225 horsepower
- A highly tuned suspension system for responsive handling
- A bold exterior style, using a prominent, pentagonal grille and high, wide "shoulder lines" for a distinctive Acura look and feel, coupled with excellent aerodynamic performance, created, in part, by lower side sills that help reduce lift and provide stability in gusty wind conditions and at high speeds.
- A spacious interior feel and sophisticated amenities, including a state-of-the-art Acura/Bose" Music System, an Automatic Climate Control system, and other best-in-class amenities
- The added control of a Vehicle Stability Assist (VSA) system
- The latest in safety advances and technology, including a dual-stage front-passenger's air bag, driver's and front passenger's side air bags with occupant height and position sensors in the front passenger's seat, and front seat belt pretensioners
- High levels of noise isolation
- The OnStar in-vehicle communications system
The Acura 3.5 RL combines engineering excellence with an array of state-of-the-art standard luxury features and amenities.
The 2003 model is powered by an all-aluminum, 3.5-liter, single-overhead-camshaft, V-6 engine with four-valves-per-cylinder that delivers an impressive 225 horsepower. Direct ignition, Programmed Fuel Injection (PGM-FI), a three-stage Variable Induction System, and a variable silencer combine to enhance drivability, low- and high-end response, and overall performance. A balancer shaft eliminates primary couple vibration, resulting in extremely smooth engine operation at any rpm. The V-6 performs so efficiently it qualifies the RL for Low Emission Vehicle (LEV) status.
Complementing the powerplant is an electronically controlled, 4-speed automatic transmission that uses Acura's innovative Grade Logic Control system to provide exceptional shifting smoothness. Grade Logic Control greatly reduces gear "hunting" when driving uphill and enhances engine braking on downhill slopes. A gated shifter gives the driver an intuitive sense of shifter position.
The front-wheel drive powertrain design provides lighter weight, enhanced packaging efficiency, and handling stability. The 4-wheel independent double-wishbone suspension has been carefully tuned to deliver handling quickness and stability without sacrificing the RL's renowned ride quality. Special low-friction suspension joints and dampers provide superb ride compliance while hydraulic bushings in the rear suspension reduce road noise. For 2003, the RL is fitted with redesigned alloy wheels with a new polished-look finish.
The steel unit-body chassis of the 3.5 RL is lightweight yet extremely rigid, offering superb resistance to vibration while also providing a firm platform on which to maximize the performance of the suspension system.
The RL offers the very latest in safety advances and technology, including a dual-stage, front-passenger's air bag, driver's and front passenger's side air bags with occupant height and position sensors in the front passenger's seat, and front seat belt pretensioners.
The OnStar system offers the latest in-car communications and services available.
The available Acura DVD-Based Satellite-Linked Navigation System - which incorporates a Global Positioning System (GPS) receiver, an on-board inertial guidance system, and a detailed mapping database that stores route information for the continental United States on a single high-density DVD - can guide the driver to virtually any destination with ease and precision. The 7-inch liquid crystal display (LCD) screen is mounted in the upper section of the center console for easy viewing
The 3.5 RL comes equipped with an unusually long list of standard luxury features, including an interior trimmed with soft leather and burled camphor wood; an acoustically tuned, eight-speaker Acura/Bose® Music System (including a six-disc, trunk mounted CD changer), a HomeLink™ remote control system; an automatic climate control system; and a slide and tilt power moonroof. For 2003, the RL is available with a new interior color, Slate, and with three new interior wood trim finishes, Black Camphor, Golden Camphor and Auburn Camphor.


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30. Re: ePeriodicals Request Thread (eNewspapers/eMagazines)TrevorS Oct 26, 2010 4:05 PM (in response to Paginate)
Did someone forget to convert this thread to the NOOKPeriodicals?
Or is this the last solitary refuge of the lower case e?
I miss you, little e...
31. Re: This is the NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)COCyclist Oct 28, 2010 12:30 PM (in response to Michael-V)
Bicycling Magazine
32. Re: NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)dino_v Oct 28, 2010 12:34 PM (in response to Michael-V)
I would literally kill to see Fortean Times on my NOOK!
I know a lot of the editorial team over there, too, if you need some contacts!
33. Re: NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)jslakey Oct 28, 2010 12:43 PM (in response to dino_v)
I know there is this question already in the Nookstand or whatever it's called. But will the new offerings of magazines and newspapers be available for both nooks?
34. Re: NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)amberfl Oct 28, 2010 12:43 PM (in response to Michael-V)
Everyday Food
Entertainment Weekly
US Weekly
35. Re: This is the NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)DogStreetBookGuy Oct 29, 2010 11:38 AM (in response to flyingtoastr)
I'd love to see the following magazines available for the Nook:
Black Static
Cemetery Dance
Rue Morgue
Dark Discoveries
Realms of Fantasy
Shock Totem
Fortean Times
The Week
The Strand
The Writer
Writer's Digest
Playboy, and other men's magazines.
This is a pipedream, for sure, but what about out of print magazines? I'd love to be able to read back issues of OMNI Magazine
36. Re: This is the NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)DogStreetBookGuy Oct 29, 2010 11:39 AM (in response to flyingtoastr)
Oh, yeah!
I forgot to mention:
The Magazine of Fantasy & Science Fiction
37. Re: NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)nedrey Oct 29, 2010 7:49 AM (in response to Michael-V)
i second or third, Wired, Entertainment Weekly
Would also like to see Vanity Fair
38. Re: NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)fourgold Oct 30, 2010 1:36 PM (in response to Michael-V)
Oooo- another vote for the Atlanta Journal Constitution !
39. Re: NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)htbyron Nov 1, 2010 9:35 AM (in response to Michael-V)
Wired & Scientific American, for the NookColor
The Economist, for both
And please update the Android nook app so I can read my subscriptions on my phone as well as my nook!
40. Re: NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)JA_Bal Nov 1, 2010 11:01 AM (in response to Michael-V)
- Readers Digest
- Writers Digest
- WestBranch (literary magazine)
- Fantasy Magazine
- Philadelphia Inquirer
- Courier Post
41. Re: NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)Dawn_G Nov 2, 2010 7:31 AM (in response to Michael-V)
New Scientist
US Weekly
TV Guide
42. Re: NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)Brighid_Moret Nov 2, 2010 10:22 PM (in response to Michael-V)Parents Vogue knitting Spin Off Lapidary Journal New York Review of Books Bookmarks Muscle & Fitness Hers Pregnancy Journal CondeNast Traveler Budget Travel Washingtonian
43. Re: NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)Haleakala_LKB Nov 5, 2010 8:59 AM (in response to Michael-V)
The Weekly Standard -and thanks for asking.
Already reading on Nook for PC and Android, can't wait till Christmas and a real Nook!
(and if no one got my heavy hints and buys me one, I'm getting it for myself!)
44. Re: NOOKperiodicals Request Thread (NOOKnewspapers/NOOKmagazines)WarBrand Nov 5, 2010 6:53 PM (in response to Haleakala_LKB)
Greensboro News and Record


text: OverviewBack to top Nutritional Therapeutics - Healthy Curb with NT Factor - 90 Tablets CLEARANCED PRICED Item# :71861UPC# :703435700902 Brand:Nutritional Therapeutics Size/Form:90 Tablets Ship Weight:0.25 Servings:45 Dosage:2 Tablet(s) Click here to read a PDF brochure on this product Nutritional Therapeutics Healthy Curb with NT Factor - 90 Tablets Nutritional Therapeutics Healthy Curb with NT Factor is made entirely from food, with no stimulants, herbs or chemicals. It curbs your appetite, so you eat less. Healthy Curb is a kick-start to weight loss, and is a perfectly EASY way to manage your diet. Healthy Curb is a food-based supplement that actually builds your cell walls, providing you with more energy while curbing your appetite for bad food. 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It is composed only of food and food components listed as: Phosphoglycolipids - includes polyunsaturated phosphatidylcholine, glycolipids and other polyunsaturated phosphatidyl nutrients. Bifido and Lactobacillus Bacterium - freeze-dried and microencapsulated in a state of suspended animation with the potential to form healthy microflora colonies. Growth Media - foods and bacteria growth factors to support microflora colonies including rice bran extract, arginine, beet root fiber, black strap molasses, glycine, para-amino benzoate, leek, pantethine (bifido growth factor), taurine, garlic, calcium borogluconate, potassium citrate, spirulina, bromelain, natural vitamin E, calcium ascorbate, alpha-lipoic acid, oligosaccharides, B-6, niacinamide, riboflavin, inositol, niacin, calcium pantothenate, thiamin, B-12, folic acid, chromium picolinate. Their ScienceThe NT Factor's unique cutting edge Antioxidant Therapy is a powerful Healthy Aging strategy. 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All biomembranes are composed of a varying ratio of proteins and phospholipids. It is the phosphoplids that are damaged and change over time (due to free-radical attack & oxidization). Anti-oxidants work to try to slow down this activity by neutralizing free radicals that damage membranes. However, if damage was already done, then traditional anti-oxidants have little ability to repair that damage. Now, with NT Factor's Antioxidant Therapy, bad phospholipids in membranes can simply be replaced by vibrant healthy phospholipids in NT Factor. That effectively returns the health of the membranes, in the case of the seventy-year-old study participants, to a level of vitality, integrity and function more than half a lifetime earlier, all leading to a successful healthy aging strategy. A study was conducted on people with moderate and severe fatigue. Within eight weeks, there was a 40% reduction in fatigue, as measured by the Piper Fatigue Scale. The research measured results at 4 weeks, 8 weeks and 12 weeks. The research indicated a loading dose over the first two months, followed by a maintenance dose thereafter. The research used the following dosing: Months 1 and 2: 2 tablets with each meal or 6 tablets per day; Month 3 and beyond: 1 tablet with each meal or 3 tablets per day. Anecdotally, physicians report that many of their patients start feeling the improvement within 2-3 weeks. It has now been proven in human clinical trials that the nutrient compound will restore lost membrane vitality. In an elderly population, individuals consuming six tablets of NT Factor per day restored their membrane health to levels normal for young healthy adults in just two months. Researchers from the University of California at Irvine, and the Institute for Molecular Medicine, call this achievement "Lipid Replacement Therapy". Referring to membranes that had been damaged by free radicals, researchers found that using this new technology healthy cell components could simply replace dehydrated and deteriorated membrane. The end result was that in as little as eight weeks, study participants whom had an average age of 70 years, regained membrane activity normal for healthy young adults. Researchers simultaneously evaluated the participants to determine if they had a corresponding relief of fatigue or sense of tiredness. The doctors found that individuals experiencing moderate or severe fatigue at the start of the study, had little or no fatigue at the end of eight weeks. NT Factor Questions & AnswersWhat is NT Factor?NT Factor is a nutrient compound composed of phosphoglycolipids (the highest functioning fats in human body), with pre- and pro-biotics. These phosphoglycolipids are the building blocks of cell membranes, and the pre and pro-biotics work to balance friendly bacteria in your large intestine. How does it work? NT Factor works by repairing cellular membrane function, and balancing a healthy microflora. We all have something in our bodies called Phosphoglycolipids that are the building blocks and fabric for healthy cells. Over time those lipids become damaged, and what NT Factor does, is replace those damaged lipids, with healthy undamaged lipids. It also provides the nutrition for restoring healthy bacteria in our gastro-intestinal environment.Why is NT Factor important to my health?NT Factor rebuilds and repairs the membranes in your cells, the building blocks of health. In the membranes of your cells and mitochondria is where food, air and water become energy you can use (ATP), where hormones, enzymes and many proteins and fats are synthesized for use in your body. In clinical trials NT Factor returned the health of cells in a 70 year old population to a normal level for healthy young adults (control group had average of 29 yrs) in 8 weeks. No other product they know about can make that claim. They have spent years testing and perfecting this science; 5 clinical trials, more than 2-dozen review papers and 38 million doses consumed to date. Is NT Factor safe?NT Factor is made 100% from food extracts. It does not use foods that contain naturally occurring stimulants or hormones. To date approximately 38 million doses of NT Factor have been consumed. In less than 1% of those doses, there have been complaints of an increase in flatulence and/or loose bowels, which is normal due to the pre and pro-biotics in the product. Most of these complaints resolved themselves naturally within the first few weeks of taking the product. It contains no harmful chemicals, caffeine, stimulants, no additives or preservatives, no sugar, wheat, dairy, nuts, gluten or toxins. Is NT Factor gluten free?Yes, NT Factor is gluten and wheat free. Will NT Factor interfere with any medications?Generally not, unless the drug instructional insert indicates otherwise. This is required by federal law to be provided in every prescription. Has NT Factor been tested?NT Factor has been tested extensively over the last 18 years. It has been studied in 5 peer-reviewed published clinical trials, including double blinded placebo controlled randomized crossover trial design. All trials were supervised by a registered, or university affiliated, IRB (Institutional Review Board). Reviews have been published in more than 12 medical journals. Should NT Factor be taken with food?NT Factor products may be taken with or without food. Since NT Factor products contain a wide range of foods and probiotics, all the needed coenzymes and cofactors are either present or created as a metabolite. Can I take too much of NT Factor?No, the reason is NT Factor provides two types of nutrition; cell membrane nutrition and gastro-intestinal flora nutrition. As NT Factor increases the saturation of cell membrane it continues to improve the health and function of the membranes. If there is too much at any one time it won't make it into the membrane matrix, and so, will be shuttled off to provide nutrition such as neurotransmitter aids and short chain fatty acid nutrients. In the gastro intestinal tract the bacteria will simply mature in the large intestine and go through its metabolic life. Is NT Factor a vitamin?NT Factor contains small amounts of some vitamins but it should not be considered a vitamin supplement. The small amount it provides is consumed by the friendly bacteria present in the group. NT Factor is a cell rebuilder. They do provide daily vitamins that are included in many products such as "Propax with NT Factor" and "Propax Gold with NT Factor". Check them out on their website. One packet a day can give you everything you need to rebuild your cells and feed them with the nutrients they need. Is NT Factor an antioxidant?All nutrients except iron are anti-oxidants. NT Factor is a group of nutrients completely extracted from food and does not contain any naturally occurring stimulants or hormones. NT Factor does contain some antioxidants however that is not its method of action to improve the health and vitality of people. It works by providing cell membranes with the building block to restore their own vitality and repair damage done previously by free radicals. (This is not something that anti-oxidants alone can accomplish.) Then it provides the nutrients (pro-and pre-biotics) to restore healthy microflora (healthy bacteria) colonies in the gastro intestinal tract. NT Factor is unique and novel because it can provide the cell membrane nutrients that are documented to match human membrane, and are not oxidized/digested when consumed. How does NT Factor work in combination with other supplements such as vitamins, minerals, herbs and foods?NT Factor will make other nutrients more effective because it is proven to improve membrane potential. That refers to the electrical activity of a membrane and that drives metabolism. How will it help my fatigue and tiredness?In five clinical trials it has been proven that will reduce and eliminate fatigue regardless of the person's age or severity of fatigue. Because it requires no external energy to work it will work regardless of the person's age or health. What separates NT Factor from other products that claim to fight fatigue? NT Factor is the only product proven in clinical trials to reduce and eliminate fatigue. No other product had been proven to provide the same benefit. Some products create the illusion by discussing aspects of the energy producing process, or the oxidation and/or transport of pre-energy compounds, like sugar and/or fats. However, NT Factor is the only supplement to actually be evaluated successfully for fatigue. How long will it take for me to see and feel benefits from taking it?Surveys indicate people feel the benefits beginning within three days. Clinical trials show dramatic results continuing throughout two months. What are the age groups that can benefit from this technology?Cellular damage has been thought to begin occurring after puberty, so it would be valuable for that age group and older. What does it mean that it can cut your biological age in half?The most credible theory of how aging happens is called the 'Mitochondrial Clock Theory of Aging". This theory states that the damage done in our bodies to the mitochondria in our cells determines how well, and how long, we can live. In a recent clinical trial, participants who were 70 years old took NT Factor for eight weeks, and at the end of those eight weeks their mitochondrial function (cellular function) was equal to that of a healthy 29 year old. The improvement is sustainable so long as the person continues to use NT Factor, according to the published study. The key measurement was the mitochondrial membrane potential. How does NT Factor differ from other nutrients that claim to help cellular function?NT Factor provides the ability to restore lost membrane function. Typically, nutrients can help to mitigate future damage but only NT Factor is proven to be able to restore lost membrane function. An example is that we expect older people to have less energy. In one clinical trial NT Factor products restored the energy producing ability (called mitochondrial function) in elderly people to levels considered normal for young healthy adults. About Nutritional Therapeutics Inc. (NTI)At Nutritional Therapeutics Inc. (NTI), their reputation is closely linked to the purity, balance, and integrity of their products.For nearly three decades, the management team at Nutritional Therapeutics Inc. has been in the business of developing, manufacturing, and distributing both OEM, and proprietary formulations of nutrition supplements for healthcare professional, and consumer markets throughout the world. They produce only natural products with scientifically formulated food ingredients, thereby avoiding additives found in other dietary supplements and pharmaceutical products. All of their tablet and capsule products make use of NT Factor™, their proprietary tablet technology, which [1] contributes to the nutritional value of their products, [2] serves as a superior bio-delivery system and [3] provides the base for an all-natural and complete food in every tablet and capsule they produce. They presently manufacture over 100 different product formulas. These are all products that foster good health, and help to manage a variety of different chronic disorders, including allergies, cardiovascular disease, diabetes, and cancer. To support their product development, they perform continuous research and development, and participate regularly in groundbreaking studies. This is done with the intent of bringing the most nutritionally complete, and healthful products to their customers.


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Cornelia Jefferson Randolph
From Thomas Jefferson Encyclopedia
Born at Monticello, Cornelia Jefferson Randolph (1799–1871) was the fifth child and third surviving daughter of Martha Jefferson Randolph and Thomas Mann Randolph. Cornelia spent much of her time at the home of her grandfather Thomas Jefferson and, as a young girl, often accompanied him on visits to Poplar Forest. She learned mechanical drawing from Jefferson and practiced by creating renderings of architectural plans for the University of Virginia. When she was a teenager Cornelia gave John Hemmings a dictionary to aid him in learning to read and write. [1] Cornelia never married and lived at Tufton and then Edgehill, the homes of her older brother Thomas Jefferson Randolph. In the 1830s, in order to try to improve the family finances, a school was established at Edgehill where Cornelia taught drawing, painting, and sculpture. She later translated and edited The Parlor Gardener: A Treatise on the House Culture of Ornamental Plants. Translated from the French and Adapted to American Use (Boston, 1861). After the Civil War, Cornelia moved to Alexandria, Virginia, to live with two of her sisters at the home of her niece Martha Jefferson Trist Burke. She died there on 24 February 1871 and was buried in the Monticello Graveyard.
- ↑ Cornelia Randolph to Virginia Trist, Oct. 25 [1816] (Nicholas Philip Trist Papers).
Further Sources
- Randolph, Cornelia. Commonplace Book. n.d. Library of Congress, Thomas Jefferson Papers. http://memory.loc.gov/cgi-bin/ampage?collId=mtj6&fileName=mtj6page059.db&recNum=139
- Thomas Jefferson Foundation, Inc. Family Letters Project. http://familyletters.dataformat.com
- Malone, Jefferson, 6:286.
- Shackelford, Descendants, 1:147–53, 253.


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A caricature artist will delight guests by quickly drawing their likeness! A caricature artist can also provide drawings and illustrations for books, comics, t-shirts and more! Search here for Caricature Artist in Las Cruces.
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Have you thought about Science or Math Education for a career?
Do you want to help kids right here in Utah?
If you answered yes to these questions the Noyce Scholarship might be for you!
The Robert Noyce Teacher Scholarship Program seeks to encourage talented science, and mathematics majors to become K-12 mathematics and science teachers. This scholarship provides $10,000 per year for Junior and Senior Biology, Earth Science, and Mathematics Secondary Education majors. The awardees commit to teaching two years in high-needs school districts for each year they receive the Noyce Scholarship.
There is still time to apply for the 2013-2014 award year!!! Applications will be accepted until August 30th.


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I've gotten myself a job as a tattoo artist! I really enjoy it
I'll upload some of my work here, but it's really late now, so I'll just show a few for the moment.
Note: It's jobs that people ask me to do, so it's nothing personal; I'm hinting towards the first image.
They're just sketches.


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Quick Quiz: Which town has the lowest murder rate?A. Omaha, NebraskaB. Toledo, OhioC. Tepoztlan, Morelos, MexicoD. Kokomo, Indiana Okay. Trick question. There are two right answers: Tepoztlán is tied with Kokomo for the lowest rate over Omaha and Toledo. What? It can't be. Is crime out of control in Omaha and Toledo? Will Anderson Cooper […]Read More
- Home
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- Tepoztlan: Our Home
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Welcome to your morning dose of denim news, links, sales, and other irresistible glimpses of inside info. Catch up on what's happening (and what's definitely not happening) and browse our personal picks after scanning the web this morning. Got a tip you think we should include, a sale you want to share, or a style you just saw and can't live without? Email us!
1. For a perfectly Valentine's Day-themed look, Glamour styled Rachel McAdams in cute printed hot pants and a bright red denim shirt for the cover of their February 2012 issue. So much love (in more ways than one)!
2. JFour recently released their spring/summer lookbook, but we love their great behind the scenes photos–in black-and-white, to boot–of their distressed skinnies, denim drop-crotch pants, and cropped jeans in action.
3. John Marcelo spent 2011 wearing the same pair of jeans to chart the so often ignored wear-induced evolution of denim, but he hasn't abandoned the project in the new year. Without Wash continues into 2012 with a new pair of jeans, 3sixteen's Slim Leg Black Selvedge denim.
4. Ludwig Van x Quintin Co.'s limited edition "Scout Series" was released yesterday, complete with some serious secret denim love. Their six-panel cap is made from vintage Japanese broadcloth, leather, and nylon fabric–and includes a selvage denim stash pocket inside.
5. Stylist and ex-Rachel Zoe employee Brad Goreski gushes about his love of denim shirts and denim on denim in this AfterElton interview. A boy after our own hearts!
—Brie Hiramine
Tags: 3sixteen, brad goreski, Denim On Denim, jfour, rachel mcadams, red denim shirt, Selvage Denim, without wash


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Top Definition
When you're fucking a detoxing meth. addict in the ass and he/she shits explosive diarrhea all over your chest, stomach, balls, and cock.
I paid this junkie bitch for some back-door action and wound up getting a Dundalk brown out.
by jlid July 28, 2010
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Rensselaer County Scoping Comments
Berkshire Regional Planning Commision Scoping Comments
Community Health Registered Nurse Job Announcement
Welcome Message from County Executive Kathy Jimino
2016 State of the County
Click on the image above to learn about New York Alliance For Donation.
Click on the image above to learn about $aving energy in your home.
Dear Guests,
Welcome to Rensselaer County's Internet home page. If you live here, we hope you are able to access the services you need, and if you don't live here we hope you have similar good fortune and encourage you to come visit us...who knows...you may decide to stay.
As one of the earliest of New York's counties, Rensselaer has a population of about 153,000 residing over 665 beautiful picturesque square miles. The quality of life as portrayed in our 14 towns, six villages, and two cities, lends to the air of spirit and togetherness symbolic of what life should be about. The County seat is Troy, known as the home of Uncle Sam and our other city, Rensselaer, sits on the bank of the historic Hudson River, directly across from the Empire State's Capital, Albany.
The county slogan, "Life Looks Good From Here" is consistent with our identity. We are an excellent place to live, to work, to shop, to raise a family, and yes, to start a business. The economic, educational, and recreational opportunities to be found in our County are second to none. And our refreshing blend of rural and hi-tech influences makes us truly appealing.
"Convenience" could be a secondary slogan for us. We are located within 250 miles of New York City, Boston, Montreal, and Buffalo, placing us in the hub of North American activity, and are easily accessible to the New York State Thruway and well as cialis the Taconic Parkway. Additionally, we are home to one of the busiest yet most customer friendly Amtrak facilities in the northeast. Add to this a certain New England flavor by virtue of bordering Vermont and Massachusetts, and we indeed provide lifestyles for everyone.
I guess by now you have figured out that I am really proud of Rensselaer County...of what we have and what we will achieve in the future...but don't let me influence you...for more information you are encouraged to follow links on this page for instant connection to Rensselaer County services, cultural and recreational opportunities, colleges and universities...and much, much more.Thanks for your visit.


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Options: We offer you the options to add LED lights to any of our fluffies, instantly upgrading you to light-up fur. Want to add a little more spice to your legwarmers? Add a ribbon or two to give your pair a unique look! Don't forget to add matching furry wrist cuffs as well to complete your outfit!
Sizes: All of our fluffies are one-size fits all but we can take custom orders for an additional fee to get you the exact fitment for your body type. Feel free to inquire about our custom petite and plus size options.


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NIFA Honors Outstanding Grantees with Partnership Awards
Every time we close out a fiscal year and report on the outcomes our grants have supported, I am always astounded by the vast scope of work accomplished by the thousands of grantees who have received awards from the National Institute of Food and Agriculture (NIFA). During our annual awards ceremony this week, we took time out to honor the outstanding work of the best of the best — four grantees received NIFA Partnership Awards for their exemplary work and contribution in support of the USDA mission and for their positive impacts on agriculture. Read more »