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Latest Front Page News
Cold Plasma Can Help Treat Non-Healing Wounds and Trigger Cellular Regeneration
Submitted by: Odio @ 12:58 AM | Friday, March 10, 2017 | (url: https://futurism....)
[quote]A CHILLY DISCOVERY
Category: Technology | 4 Comments
Tags: godmode
Science found 7 earth sized/like worlds in one system
Submitted by: Goshin @ 01:35 PM | Wednesday, February 22, 2017 | (url: https://www.nasa....)
NASA's Spitzer Space Telescope has revealed the first known system of seven Earth-size planets around a single star. Three of these planets are firmly located in the habitable zone, the area around the parent star where a rocky planet is most likely to have liquid water.
Category: Technology | 37 Comments
Tags: science
New system makes it harder to track Bitcoin transactions
Submitted by: Odio @ 10:03 AM | Thursday, February 9, 2017 | (url: https://phys.org/...)
Researchers from North Carolina State University, Boston University and George Mason University have developed a Bitcoin-compatible system that could make it significantly more difficult for observers to identify or track the parties involved in any given Bitcoin transaction.
Category: Technology | 2 Comments
Tags: bitcoin
new form of matter discovered
Submitted by: Goshin @ 02:07 PM | Monday, January 30, 2017 | (url: http://www.popula...)
Crystals are structures in which a pattern of atoms or molecules repeats in space. Now, two teams of researchers have figured out that crystals' repeating patterns can also exist through time. These "time crystals," detailed in a new paper in Physical Review Letter, are an entirely new kind of matter, one that can never reach equilibrium.
also metallic hydrogen was created last week or somethin
Category: Technology | 27 Comments
Tags: science
Scientists Use Nanoparticles to Create a Universal Treatment for Allergies
Submitted by: Odio @ 10:28 PM | Saturday, January 7, 2017 | (url: https://futurism....)
Category: Technology | 14 Comments
Tags: orbital123
[OFN]A new method to fuck up the Carbon Cycle has been invented.
Submitted by: Odio @ 10:38 PM | Thursday, January 5, 2017 | (url: http://www.bbc.co...)
The researchers say that soda is more effective than current methods and less damaging to the environment.
Category: Technology | 23 Comments
Tags: co2
Avatar-style S. Korean manned robot takes first baby steps
Submitted by: Odio @ 03:58 PM | Wednesday, December 28, 2016 | (url: http://phys.org/n...)
A giant South Korean-built manned robot that walks like a human but makes the ground shake under its weight has taken its first baby steps.
Category: Technology | 11 Comments
Tags: robots
New York officials report the first ever case of bird flu spread from cat to human
Submitted by: Odio @ 03:17 PM | Wednesday, December 28, 2016 | (url: http://www1.nyc.g...)
One person, of more than 350 people screened, has been found with H7N2; this person is a veterinarian who had prolonged close exposure to respiratory secretions of sick cats at Animal Care Centers of NYCs (ACC) Manhattan shelter and has recovered from mild illness
Category: Technology | 14 Comments
Tags: plague
Ocean temperatures faithfully recorded in mother-of-pearl
Submitted by: Odio @ 08:23 PM | Friday, December 16, 2016 | (url: http://phys.org/n...)
Mother-of-pearl or nacre (pronounced nay-ker), the lustrous, tough-as-nails biomineral that lines some seashells, has been shown to be a faithful record of ancient ocean temperature.
Category: Technology | 2 Comments
Tags: science
Earths crust is getting thinner than ever, thanks to a rapidly cooling interior
Submitted by: Odio @ 08:35 PM | Wednesday, December 14, 2016 | (url: http://www.scienc...)
If you looked back at Earth 170 million years ago, youd find a very different planet. The worlds continents were all linked up into one vast 'supercontinent' called Pangaea, and according to a new study, the outermost layer of the planet was 1.7 km (1 mile) thicker than it is today.
Category: Technology | 12 Comments
Tags: science.and.industry
Non-OPEC countries agree to cut oil production by 558,000 barrels per day
Submitted by: Odio @ 03:13 PM | Saturday, December 10, 2016 | (url: https://www.rt.co...)
Non-OPEC countries agreed to cut oil production by 558,000 barrels per day (b/d) during a meeting with members of the Organization of Petroleum Exporting Countries (OPEC) in Vienna.
Category: Technology | 38 Comments
Tags: oil
Early explorer logbooks reveal Antarctic sea ice has barely changed in 100 years
Submitted by: Odio @ 08:46 AM | Friday, November 25, 2016 | (url: http://www.scienc...)
Logbooks from the likes of Captain Robert Scott and Sir Ernest Shackleton - key figures in the 'Heroic Age' of Antarctic exploration - have revealed that sea ice levels in Antarctica have barely changed over the past century, despite global temperatures hitting record highs year after year.
Category: Technology | 98 Comments
Tags: globalalarming
The IRS Just Declared War on Bitcoin Privacy
Submitted by: Odio @ 05:54 PM | Tuesday, November 22, 2016 | (url: https://fee.org/a...)
The Internal Revenue Service has filed a John Doe summons seeking to require U.S. Bitcoin exchange Coinbase to turn over records about every transaction of every user from 2013 to 2015. That demand is shocking in sweep, and it includes: complete user profile, history of changes to user profile from account inception, complete user preferences, complete user security settings and history (including confirmed devices and account activity), complete user payment methods, and any other information related to the funding sources for the account/wallet/vault, regardless of date. And every single transaction:
All records of account/wallet/vault activity including transaction logs or other records identifying the date, amount, and type of transaction (purchase/sale/exchange), the post transaction balance, the names or other identifiers of counterparties to the transaction; requests or instructions to send or receive bitcoin; and, where counterparties transact through their own Coinbase accounts/wallets/vaults, all available information identifying the users of such accounts and their contact information.
Category: Technology | 18 Comments
Tags: fuck irs
Scientists Are Bringing Back Vacuum Tubes for Computers of the Future
Submitted by: Odio @ 04:21 PM | Tuesday, November 8, 2016 | (url: http://www.popula...)
Researchers from UC San Diego are using vacuum tube technology to develop more efficient computer processors. The research could result in faster microelectronic devices and better solar panels. Their results are published in a paper in the journal Nature Communications.
Category: Technology | 16 Comments
Tags: computers
Space! EM Drive really does work
Submitted by: Goshin @ 02:41 PM | Monday, November 7, 2016 | (url: http://www.nextbi...)
MAN, it's fun being right
Category: Technology | 24 Comments
Tags: space


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The People of New Day
New Day / Blog
Uduhenzagire, mana!
Turaguhimbazaa, mana!
(Bless us, O God!
We need You!
We love You!
We worship You, God!)
As a Master of Sacred Music student at Perkins School of Theology, the music of the global Church is an integral (and now expected) part of my course of study. In our very first class session with Dr. Hawn, we started learning to sing the faith in Swahili, Shona, Zulu, Spanish, Portuguese, etc. Thus, when I received the opportunity to serve as a Music and Worship Intern for New Day Upendo, a micro-community meeting at, and engaging with, an apartment complex in Dallas where many African refugees are placed upon arrival to the United States, I felt like I was going in with an advantage. Sure, there would be things to learn, but hey, I already know 10+ songs in a variety of African languages, and we could at least start there while I build relationship and learn from what they've been singing.
Well, I learned a couple things very quickly:
1. There are a few songs that seem to have traversed the African continent and have probably been translated into almost every language. Just because you know that song in Shona (Zimbabwe), does not mean they use the same words in Kenya.
2. A lot of what I had been exposed to is either in Shona or Zulu (South Africa). The majority of the people I serve are not from Zimbabwe or South Africa. If I wanted to sing something and have it understood, it was time to learn songs in Swahili and/or translate songs into Swahili. Not being a Swahili speaker (let's face it, I'm not a great Swahili singer), I often find myself running up to my friend Peter and asking, "How do you say 'always' in Swahili?" [If I ever go to Africa, I may not be able to say much, but I've got "Daima tunaomba (Always, we pray)" and "Hakuna Mungu kama wewe (There's no one like Jesus)" down!
These two lessons were really just the pre-requisites for the real course I was about to learn in what I will call "the hospitality of song." But, more on that in a moment. As I was starting to feel comfortable with translating and learning songs, and mixing the languages we sang in our gatherings, we had two women and their children start coming to New Day just a few days after their arrival in the United States. These dear women speak very little Swahili, even less English, and their dominant language is Kinyarwanda. Thankfully, we have a few people in our community who can understand and translate between English and Kinyarwanda, but this language barrier has at times provided a new challenge for many of us. One particular evening, we did not have anyone in the room capable of translating the lesson into Kinyarwanda, and a man walks in who heard us singing and wanted to see what was going on. None of us had seen him before, but in he walked, and he turned out to be a very fluent speaker of both English and Kinyarwanda. He was willing to translate for the women, and even participated in the discussion himself. It was such a beautiful moment of seeing God provide.
As a leader of song, I now have the challenge of finding songs in Kinyarwanda, which absolutely is not going to happen by looking at publications in the United States. I mean, let's face it, Swahili is at least an option on Google Translate, Kinyarwanda isn't. So, Peter starts experimenting with adding Kinyarwanda to some of the songs we sing. Then, last week, Peter remembered a song he learned in Kinyarwanda, the song that I posted at the top. After spending a week singing the song at any opportunity--in the car, the shower, while shelving books (quietly)--I taught the song to the community yesterday. Somewhere along the way as I learned and became familiar with the song, it also seeped into my spiritual bones. It started to gain meaning beyond what the words actually say. Then, to hear all of us, people of many languages, ages, experiences, and cultures, come together and sing in a language few are familiar with so that we could sing in solidarity with our sisters...it was beautiful. I realized in that moment and upon reflection that they shared something with me of such worth I can barely describe it. They shared with me the language of their heart, and they let me take it in as my own heart song. I was presented by Peter and these women a song that Means deeply, and in teaching it to me, they offered me the hospitality of song. They invited me into their musical and linguistic home, and continue to allow me to stay a while to rest, to learn, to grow, to cry, to shout, to be. I am speechless and overwhelmed at the depth of such hospitality.
Praise be to God. Amen.
Reposted from The Road Less Traveled By . . . Or Rolled Along
- Blog
- Overview
- History & Mission
- Rule of Life
- Video
- Role of the Anchor Church
- Worshipping Communities
Pilgrimage is a journey to a place where an event of religious significance occurred or where objects of religious significance are housed. As a discipline to promote spiritual transformation, it is an ancient activity associated with many of the world's religions. In our efforts to train Christian leaders for the future, the Missional Wisdom Foundation embraces pilgrimage as an important avenue of spiritual formation.
Conversations with Elaine
Dr. Elaine Heath talks about how missional work is transforming what it means to be a Christian disciple.

subset: commoncrawl


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It's nice how immediate the relationship is between musicians and the internet. Like just now, an unmastered, untitled, and unmixed track just washed up on Joker's twitter – because why not? It certainly makes our Thursday afternoon a little more exciting. Joker is toying around with house strings, brain-tickling drums, and a killer verse via Bugsy – there's none of the low-end rumble that defined a song like "Tron." Is this the producer's next evolution? I suppose only time will tell – take a listen below.
Joker & Buggsy – Music (Unmixed/Unmastered) [No Label] by frankyboy


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from £85 / night help Price for guests, Nights approx:
2 Bedroom Condo Vilar do Golf, Quinta do Lago – Home 6789018 Apartment
- 2 bedrooms
- 6 sleeps
- 1 night min stay
2 Bedroom Condo Vilar do Golf, Quinta do Lago – Home 6789018
- Apartment
- 2 bedrooms
- sleeps 6
- 1 night min stay
Top Review
Apartment / 2 bedrooms / 1 bathroom / sleeps 6
Key Info
- Beach / lakeside relaxation
- Nearest beach 2 km
- Swimming pool
- Child friendly
- Car advised
- Air conditioning
- No pets allowed
Description from manager
Located in the heart of acres of countryside, where pine forests that are centuries old come together with the Atlantic Ocean is Vilar do
Golf, where you will yourself surrounded by lush gardens, amazing golf courses, and some of the finest leisure and sports facilities in the
Our stylish and comfortable 2 Bedroom Apartments are fully air- conditioned and feature a modern bathroom with a Bath and Shower, and
Wash basin with plenty of space for all of your lotions and potions. There is also a hair dryer, a complimentary amenity kit, shaver point
and a heated towel rail. The Double Rooms and Twin Rooms are bright and airy with bedside tables with an Alarm Clock and plenty of
room to put your belongings. There is also a Sofa Bed should you need.
The fully equipped Kitchen offers a Fridge, Microwave, Mixer, Oven, Toaster, Coffee Maker, Crockery and Utensils, Dishes, Glassware,
Hob, Juicer, Kettle, Dishwasher, Washing Machine/Tumble
Located in the heart of acres of countryside, where pine forests that are centuries old come together with the Atlantic Ocean is Vilar do
Golf, where you will yourself surrounded by lush gardens, amazing golf courses, and some of the finest leisure and sports facilities in the
Our stylish and comfortable 2 Bedroom Apartments are fully air- conditioned and feature a modern bathroom with a Bath and Shower, and
Wash basin with plenty of space for all of your lotions and potions. There is also a hair dryer, a complimentary amenity kit, shaver point
and a heated towel rail. The Double Rooms and Twin Rooms are bright and airy with bedside tables with an Alarm Clock and plenty of
room to put your belongings. There is also a Sofa Bed should you need.
The fully equipped Kitchen offers a Fridge, Microwave, Mixer, Oven, Toaster, Coffee Maker, Crockery and Utensils, Dishes, Glassware,
Hob, Juicer, Kettle, Dishwasher, Washing Machine/Tumble Dryer, Iron and Board and many other conveniences.
The open plan Living area has comfortable Sofas and Chairs with a Coffee Table, Cable TV and DVD Player. There is a book and DVD
Library on site for you to make the most of! The Dining area has seating for 6 and beautiful wooden Dining Table. The Apartments feature a
lovely balcony, patio or terrace and are fully air conditioned.
It doesn't matter if you are looking for a romantic getaway for two, or a fun-filled family vacation. Vilar do Golf has everything you could
ever want in a vacation destination. There are plenty of outdoor activities that the entire family can enjoy, such as Aqua aerobics,
Badminton, Basketball, the Game Room, Outdoor Playground, Pool table, Table Tennis/Ping pong, Volleyball or even relax and make use
of the Sauna and Spa. There are Four Pools on site in total; an Outdoor Swimming Pool and Childrens Pool and an Indoor heated Pool and
even a Whirlpool!
You can rent bicycles and explore the beautiful country side on the Bike Trails, or even one step further and rent Scooters for the day!!
There is a Fitness Center on site, lush Gardens, and of course Championship Golf Courses – why not take a swing!!
Vilar do Golf is known as a golfer's paradise, with Quinta do Lago's 18-hole championship courses in the Ria Formosa Nature Reserve.
There are also a number of beautiful parks and gardens to explore, as well as museums for those looking to add a little bit of culture and
history to their vacation.
Along with family activities, there are many adult-oriented activities to enjoy. For instance, you may want to visit the fun and exciting
nightclubs to dance your nights away, or enjoy shopping and fine dining featuring area cuisine and many other delicious dishes.
Wifi Available. Safe, Cots, High Chairs and Baby Gates available locally.
Distance from Resort: Beach: 2 km; Marina: 10 km; Shops: 2 km; Supermarket and Restaurant : On-site
Wifi charged at €5.95 per day
Additional fees may apply for use of leisure centre and resort facilities
All requests are subject to availability and cannot be guaranteed
Location description from manager
The Algarve region
The Algarve stretches across the whole of southern Portugal. Its name derives from the Arabic El-Gharb meaning "west". Although the Algarve has been occupied by the Moors for 600 years, besides some city- and castlewalls in Silves and Tavira, there are hardly any remainders.
Nevertheless all houses in the Algarve are still being build with those typical arabic chimneys. The Romans however left several bridges and ruins East of Faro the coast is known as the leeward shore. The area stretching eastwards to the Spanish border forms an unusual lagoon landscape closed by sandy islets. A great part of the coastline has been declared a protected area, namely Parque natural da Ria Formosa. The windward shore is the Algarve's most famous stretch of coast. Ochre - coloured cliffs plunge down to the beach and the turquoise sea surges into coves and grottoes.
The interior of the Algarve is still relatively unknown. The flower-decked, whitewashed villages have managed to preserve their traditional appearance, where handicrafts are still an important industry for the local population. The year-round scents alone are worth the visit, with eucalyptus, pine, lavender and rock-rose all growing here in abundance.
Quinta do Lago
Local Activities - Everything is possible in Quinta do Lago!
Locally you can find anything; Beaches, Bike Trails, Boating/Sailing, Casinos, Cinemas, Fishing, Driving RangeGolf - Pro shop Golf - Putting Greens, Horseback Riding. Jet Ski Rentals, Lakes, Marinas, Mini Golf, Moped/Scooter Rentals, Mountain Biking, Museums, Nightlife, Parasailing, Parks, Restaurants, Scuba Diving/Snorkeling, Shopping, Squash, Tennis, Theatres, Water Sports, Whale watching and even Windsurfing just to name a few!
Quinta do Lago on the southern coast of Portugal is one of Europe's finest destinations. If you are looking for the ultimate in golfing vacations, then this is the destination for you. In fact, not only can you golf on a world-class championship course, you will love the more than 2,000 acres of beautiful countryside.
A man-made lake offers just every watersport imaginable, and, as well as the first-class golf facilities, other activities include horse-riding, tennis, squash and clay pigeon shooting. The beach at Quinta do Lago is reached by crossing a long bridge over an estuary in the Ria Formosa Nature Park and the lovely golden sands stretch along the coast to Vale do Lobo. The sandstone cliffs provide a beautiful backdrop to the wonderful sandy beach which is excellent for both relaxing sunbathing and enjoying energetic family games. A convenient bar/restaurant serves up refreshments and meals, including freshly caught fish, right on the beach. Boat trips are available from the beach at Quinta do Lago and it's possible to charter a yacht or venture out on a deep-sea fishing trip.
PRAIA DE GARRÃO beach fronts the chic area of the holiday resorts known as Quinta do Lago and Vale do Lobo. This beach can now be easily reached by road and one area popular for its beach restaurants is a section known as "Julias" - a local lady and a character who opened the first beach place at the time when access was either by horse or by a very sandy road through the pine trees.
The Natural Park of Ria Formosa starts in the ANCÃO Beach. The Beach has an extensive sandy area and a vast area with dunes. It is well known for its famous upper class frequency - the so called Portuguese Jet Set can be seen wining and dining at the famous GiGi's restaurant. The Beach is a beautiful sandy beach of unquestionable environmental quality. The area is surrounded by the Ancão Valley which is natural forest mainly of pine trees and dune vegetation.
Quinta do Lago is one of the Algarve's largest golf destinations and its courses are rated among Europe's top 25. To play golf in Quinta do Lago is a way of life for visitors. This area is one of the largest golf destinations, with over 30 outstanding courses to try. Quinta do Lago has hosted the Portuguese Open Championship eight times in addition to hundreds of national and international prestigious tournaments and continues to be a much sort after venue.
The fairways are wide and flanked at reasonable distance by the pines, heather, broom and beautiful wild flowering shrubs with several lakes in the play, providing a test to players of all ability levels. Enjoy a few rounds in the glorious Portuguese climate, with almond trees, citrus groves and fragrant breezes from the ocean adding to the atmosphere.
There are first class driving ranges, a golf academy, putting greens, pro-shops and clubhouses with large and informal terraces - the perfect setting to relax with friends and have a well earned drink or a light meal after a challenging round.
For those who love to shop, the Quinta da Lago 'Q' Shopping Centre has many exclusive designers shops and some lovely restaurants/cafés - some very upmarket ! There is also a selection of boutiques here if you really need a retail therapy fix. Hopefully you won't have to use it, but there is also a very well-equipped dispensing pharmacy here. There are also some fantastic shopping centres afield like Algarve Shopping, Forum Algarve and the shopping centre in Portimao!
More Less
- Great for children of all ages
- No pets allowed
- Cot available
- High chair available
Bed & bathroom
- 1 Sofa Bed, 4 Single Beds, 1 Cot available
- 1 Family bathroom
- Wi-Fi available
- Air conditioning
- Shared outdoor pool (unheated)
- Shared indoor pool
- Children's pool
- Jacuzzi or hot tub
- Shared garden
- Climbing frame
- Swing set
- Patio
- Balcony or terrace
- Bicycles available
- Internet access
- Central heating
- Safe
- Staffed property
- Sauna
- Table tennis
- Pool or snooker table
- Games room
- Cooker
- Fridge
- Microwave
- Toaster
- Kettle
- Dishwasher
- Washing machine
- Clothes dryer
- Iron
- High chair available
- DVD player
- Telephone
- Fax machine
- Hair dryer
- Linen provided
- Towels provided
More Less
- Parking
- Secure parking
- Not suitable for wheelchair users
- Check in time:16:00, Check out time:10:00
- If you have any questions about check-in or check-out times, please contact the owner/manager.
- Payment
- This owner does not use online booking. Please contact them and they will be able to offer you a secure way to pay for your stay. Never pay for your holiday rental by wire transfer (such as Western Union or Moneygram) as this type of payment is untraceable.
- Smoking
- No smoking at this property
- Cancellation policy
View Policy
About the manager
Villas Away Limited
- Calendar updated::
- 29 Apr 2017
- Years listed:
- 4
- Based in:
- United Kingdom
Languages spoken: English
This Apartment has 2 bedrooms, 1 bathroom and sleeps 6. It's been listed on Holiday Lettings since 29 Sep 2015. Located in Faro District, the average weekly rate varies from £440 to £1870.
The Manager has a response rate of 100% and the property's calendar was last updated on 29 Apr 2017.
Map and how to get there
Guest reviews
Help other travellers decide where to stay
This holiday home hasn't got any reviews yet. The average rating on Holiday Lettings is four out of five. This place could be newly added or perhaps it's a well kept secret. Either way, it's time to reveal all.
Build your perfect trip, with Holiday Lettings & TripAdvisor
Real opinions, real reviews
Genuine guest feedback from 100,000+ reviews
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Know where you're staying within 24 hours
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This advert is created and maintained by the advertiser; we can only publish adverts in good faith as we don't own, manage or inspect any of the properties. We advise you to familiarise yourself with our terms of use.


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Take your favorite wine or beverage of choice with you when you're on-the-go with these unique stainless steel stemless custom wine tumblers imprinted with your logo.
Insulated Promotional Wine Duffle Bag for carrying a wine bottle.
A promotional wine opener with a revolutionary new lever-pull design that allows for effortless corkscrew removal.
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An upscale custom wine & cheese board set you'll want to be showing off at the next wine and cheese party.
If you are looking for the perfect holiday gift for the wine connoisseurs on your list, then these wine accessories & insulated custom tote bags gift sets are perfect.
One-step ergonomic wine opener with foil cutter is the perfect promotional gift set.
This double custom wine gift box fits two wine bottles, two stemless wine glasses, or two tock glasses. Your gift will be presented in this laser engraved rustic wood gift box with rope handles.
Neoprene insulated promotional Custom Logo Wine Bottle Tote for convenient carry available in a variety of bright colors.
Present your gift in this laser engraved rustic custom wine gift box with rope handle. Fits a single wine bottle, two stemless wine glasses, or two rocks glasses.
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Choose this custom imprinted wine and cheese tote bag kit for your next wine and cheese gathering
Keep two bottles of wine chilled at that perfect temperature when you visit friends and family for parties.
This neoprene insulated One Bottle Custom Wine Tote by BUILT comfortably holds one 750ml to 1 liter bottle.
Be the host with the most advanced custom wine opener out there.
Neoprene insulated Two Bottle Custom Promotional Wine Tote for two bottle carry available in a variety of bright colors.
Hand out this Mini California Custom Champagne Sparkling Wine with custom etched personalization as a year-end gift.
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A fun promotional wine gift set all bottled up in a wine bottle shaped case.
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This Full Color California Custom Champagne Sparkling Wine is crafted to present elegance at your next party. Bring this custom bottle to your next event!
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A contemporary promotional gift set includes a wine opener and bottle stopper high-end wooden box.
Present this Etched California Custom Champagne Sparkling Wine Bottle with your corporate logo and personalization for a striking presentation at your next event.
Elegant design of this smooth promotional wine pourer with stopper is sure to enhance your logo.
This custom wine caddy is a fun and stylish way to "bring your own bottle"
10 oz. of delicious promotional snacks with a custom centerpiece and a choice of two premium confections.
These promotional cookie gift boxes offer a delicious opportunity to promote your brand, introduce new products or thank your special clients.
Display your graphics on the custom packaging of this tube filled with a mix of flavored popcorn. The full-color imprint leaves an opening so that you can see the delicious treats inside.
This custom gift box containing 4 famous Ghirardelli ® promotional chocolate squares is a real San Fransisco treat!
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Delicious gourmet promo jelly beans in a beautiful pen holder that can hold up to three-quarters of a pound.
The centerpiece of this awesome 8 oz. solid promotional chocolate truffle arrangement is a great place for your logo.
Business gifts are a highly-effective way to increase brand recognition and popularity. Imagine your clients seeing your logo every day while using a product they enjoy. Boost sales with business gifts that appeal to your customers. Let ePromos find the business gift promotional item that's right for you!


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The Celebration Is On!
Starting today, the participating companies of Comprehensive Network®, Inc. are embarking on a celebration to mark our 25th Anniversary of service excellence in health care and education — 1985 to 2010. And you, our dedicated professionals, are invited to celebrate with us as we celebrate you!
©2016 Comprehensive Network®, Inc.


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Location address: España, Barcelona
Number of texts: 1
The 1992 Summer Olympic Games, officially known as the Games of the XXV Olympiad, were an international multi-sport event celebrated in Barcelona, Spain, in 1992. The International Olympic Committee voted in 1986 to separate the Summer and Winter Games, which had been held in the same year since 1924, and place them in alternating even-numbered years, beginning in 1994. The 1992 Summer Games were the last to be staged in the same year as the Winter Games. Due to the end of the Cold War, these games were the first without boycotts since 1972. In fact the Olympics was the final success of the former Soviet Union (despite only part of it taking part), and biggest of the "Olympic flag" (Nations from the former USSR competed as the Unified Team, coming 1st in the overall rankings).
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JFK's Press Conference Humor (Video)
In honor of Barack Obama's first primetime press conference as president, scheduled for 8 pm tonight, have a look at this collection of John F. Kennedy's press conference quips and one-liners. As Walter Shapiro points out in TNR today, JFK used humor masterfully to disarm the press. Obama's first attempt at emulating him fell flat; we'll see tonight if he can improve.


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Austin sure knows how to pick 'em and fuck 'em.
Awsome, i wish i am there....
@ Xtrangeforeignbeauty The beautiful top is Austin Wilde. I love watching him. He fucks like a maniac!! =)
love the thick cocks and cum shots are both impressive. would love to see them flip flop
sooo sexy
Love Anthony Romero, just love him. He has a great body and personality. I would love to meet him.
So passionate and sexy... That was a beautifully intimate moment.
whos the name of the top?
I love sucking cocks and eating cum
i want a cock..
horny and heavy hung fuckers
Muito Gostoso !!!!!
Meu Jake s2
Two playful hot guys! ;)
The black guy is sexy...But it's weird because I'm not really into black guys and I'm black... lol
uff como me encantaria hacerlo con anthony romero me masturbo pensando en el
Technical problem!


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Info on the number +22502105999
Here is some general information about the number.
Country Code: 225
Country Name: Côte d'Ivoire (Ivory Coast)
Type: Mobile
Original Provider: Atlantic Telecom - Côte d'Ivoire
Validity: The number is the correct length for an Ivory Coast phone number.
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1st September 2016 - Advance Fee Fraud Scam
On 1st September 2016, telephone number +22502105999 was reported as being used by scammers to pretend to be Chris Ambrose using email address email@example.com as part of an Advance Fee Fraud Scam. Further details of this report can be found on the ScamWarners.com forum
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Filter by:
Content type:
Stage 1&2
Stage 2&3
Stage 3&4
Stage 4&5
Challenge level:
Can you fill in this table square? The numbers 2 -12 were used to generate it with just one number used twice.
Suppose we allow ourselves to use three numbers less than 10 and
multiply them together. How many different products can you find?
How do you know you've got them all?
Can you work out the arrangement of the digits in the square so
that the given products are correct? The numbers 1 - 9 may be used
once and once only.
Can you order the digits from 1-3 to make a number which is divisible by 3 so when the last digit is removed it becomes a 2-figure number divisible by 2, and so on?
This magic square has operations written in it, to make it into a
maze. Start wherever you like, go through every cell and go out a
total of 15!
There are 44 people coming to a dinner party. There are 15 square
tables that seat 4 people. Find a way to seat the 44 people using
all 15 tables, with no empty places.
Using the statements, can you work out how many of each type of
rabbit there are in these pens?
Can you replace the letters with numbers? Is there only one solution in each case?
Can you find which shapes you need to put into the grid to make the
totals at the end of each row and the bottom of each column?
This multiplication uses each of the digits 0 - 9 once and once only. Using the information given, can you replace the stars in the calculation with figures?
Find the product of the numbers on the routes from A to B. Which
route has the smallest product? Which the largest?
What do you notice about the date 03.06.09? Or 08.01.09? This
challenge invites you to investigate some interesting dates
There is a clock-face where the numbers have become all mixed up. Can you find out where all the numbers have got to from these ten statements?
In the multiplication calculation, some of the digits have been replaced by letters and others by asterisks. Can you reconstruct the original multiplication?
A group of children are using measuring cylinders but they lose the labels. Can you help relabel them?
Zumf makes spectacles for the residents of the planet Zargon, who
have either 3 eyes or 4 eyes. How many lenses will Zumf need to
make all the different orders for 9 families?
This problem is based on the story of the Pied Piper of Hamelin. Investigate the different numbers of people and rats there could have been if you know how many legs there are altogether!
Use your logical-thinking skills to deduce how much Dan's crisps and ice-cream cost altogether.
Can you complete this calculation by filling in the missing numbers? In how many different ways can you do it?
These eleven shapes each stand for a different number. Can you use the multiplication sums to work out what they are?
Katie had a pack of 20 cards numbered from 1 to 20. She arranged
the cards into 6 unequal piles where each pile added to the same
total. What was the total and how could this be done?
This challenge encourages you to explore dividing a three-digit number by a single-digit number.
Can you arrange 5 different digits (from 0 - 9) in the cross in the
way described?
Can you work out some different ways to balance this equation?
There were chews for 2p, mini eggs for 3p, Chocko bars for 5p and
lollypops for 7p in the sweet shop. What could each of the children
buy with their money?
Have a go at balancing this equation. Can you find different ways of doing it?
Cherri, Saxon, Mel and Paul are friends. They are all different
ages. Can you find out the age of each friend using the
Each clue number in this sudoku is the product of the two numbers in adjacent cells.
Try adding together the dates of all the days in one week. Now
multiply the first date by 7 and add 21. Can you explain what
Given the products of adjacent cells, can you complete this Sudoku?
Number problems at primary level that may require determination.
56 406 is the product of two consecutive numbers. What are these
two numbers?
48 is called an abundant number because it is less than the sum of its factors (without itself). Can you find some more abundant numbers?
Number problems at primary level that require careful consideration.
What is the lowest number which always leaves a remainder of 1 when
divided by each of the numbers from 2 to 10?
Look on the back of any modern book and you will find an ISBN code. Take this code and calculate this sum in the way shown. Can you see what the answers always have in common?
This problem is based on a code using two different prime numbers
less than 10. You'll need to multiply them together and shift the
alphabet forwards by the result. Can you decipher the code?
Can you put the numbers 1 to 8 into the circles so that the four
calculations are correct?
On my calculator I divided one whole number by another whole number and got the answer 3.125. If the numbers are both under 50, what are they?
On the planet Vuv there are two sorts of creatures. The Zios have 3 legs and the Zepts have 7 legs. The great planetary explorer Nico counted 52 legs. How many Zios and how many Zepts were there?
The clockmaker's wife cut up his birthday cake to look like a clock
face. Can you work out who received each piece?
In a Magic Square all the rows, columns and diagonals add to the 'Magic Constant'. How would you change the magic constant of this square?
Tom and Ben visited Numberland. Use the maps to work out the number of points each of their routes scores.
We can arrange dots in a similar way to the 5 on a dice and they
usually sit quite well into a rectangular shape. How many
altogether in this 3 by 5? What happens for other sizes?
If you had any number of ordinary dice, what are the possible ways
of making their totals 6? What would the product of the dice be
each time?
Find the smallest whole number which, when mutiplied by 7, gives a
product consisting entirely of ones.
A game for 2 people. Use your skills of addition, subtraction, multiplication and division to blast the asteroids.
Ben's class were cutting up number tracks. First they cut them into twos and added up the numbers on each piece. What patterns could they see?
Mr McGregor has a magic potting shed. Overnight, the number of
plants in it doubles. He'd like to put the same number of plants in
each of three gardens, planting one garden each day. Can he do it?
Put operations signs between the numbers 3 4 5 6 to make the highest possible number and lowest possible number.


text:
$6.99 HP - x3000 Wireless Optical Mouse - Black
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Product Description:
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Category: Computer Components
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This collection presents a set of courses and lectures on sustainability, water resource management, and alternative energy. For more information about these and other lectures, please visit the UCI Center for Unconventional Security Affairs Sustainability Series web page: http://www.cusa.uci.edu/programs-and-events/sustainability-series.
Planning, Policy and Design 132: Sustainability II
Planning, Policy and Design 139: Water Resource Policy
Seminars and Individual Presentations
How Predictable is the Climate System: Droughts, Floods, and Extreme Events
Greening Aid? Understanding the Environmental Impact of Development Assistance
Knowledge and Environmental Policy
State Capacity, Economic Crisis and Economic Reform: Implications for Sustainability
The Politics of Sustainability
The Psychology of Sustainability
Sustainability and Food Security


text:
Fremont CA Real Estate & Homes for Sale
28 Properties Found
The median home value in Fremont, CA is $905,000.
This is
higher than
the county median home value of $735,700.
The national median home value is $185,800.
The average price of homes sold in Fremont, CA is $905,000.
Approximately 60.56% of Fremont homes are owned,
compared to 35.33% rented, while
4.12% are vacant.
Fremont real estate listings include condos, townhomes, and single family homes for sale.
Commercial properties are also available.
If you see a property you're interested in, contact a Fremont real estate agent to arrange a tour today!
Sort Results:
1-25 of 28 properties displayed
Refine Property Search
Open House:
Saturday, 4/29 1:00-4:00PM
Courtesy: EstateMind Realty Mike Tay
View additional info
Beautiful Condo in a prime location. Excellent Fremont Unified School District: Elementary - James Leitch Elementary School (K-Grade 2) - WarmSprings Elementary School (Grade 3 - 6) Middle - John M. Horner Junior High High - Irvington High Many upgrades: Totally upgraded kitchen (new kitchen counter, new flooring, new cabinets, new LED recess lighting, new cooktop, oven, new garbage disposal, new automatic dishwasher,new hood/microwave); newly renovated 2nd bathroom (new lighting, flooring, amenity); updated master bath; Totally move-in ready: Save you $$$ and time doing upgrades yourself. This unit faces a beautiful common landscape area. Convenient access to I880 and I680 Upcoming Open House: Saturday 4/22 1-4pm; Sunday 4/23 1-4pm
4825 Los Arboles Fremont, CA 94536
Courtesy: Alliance Bay Realty Tina Nguyen
View additional info
Great remodeled duet home in quiet neighborhood! No H.O.A. DUES has been remodeled. Granite counter tops, new cabinets, island snack bar for a quick meal. All new stainless appliances, refrigerator stays. Bedrooms are situated around a small patio built in the center of the home, allowing much natural light into each bedroom. Double paned windows, and several sliding glass doors to allow for a cool breeze.. Enough space to park a boat. Walking Distance to Schools and Park. Easy Access to Shops, Restaurants and Hwy 880,OH Sat/Sun 1-4pm 04/22-04/23/2017
5623 Impatiens Fremont, CA 94538
Courtesy: Nihow Real Estate , Inc. Jason Huang
View additional info
Open House On Sat. & Sun., 2pm-4pm, 1 Car Garage + 1 Carport, Central A/C, Triple Pane Windows, Recessed Lights, High Ceilings ,Upgraded Flooring, Lots of natural light, Stainless Steel Appliances, Security Alarm, Walking distance to Stratford school (private), Close to Fremont BART Stations, Easy access to hwy 880 and New Park Mall
49077 Meadowfaire Fremont, CA 94539
Courtesy: Climb Real Estate Group Christine Ko
View additional info
Beautiful End Unit Home with Desirable Open Floor plan in Highly Sought after Warm Springs! Top Rated Schools, K-12. This home features spacious & open living space perfect for entertaining, surrounding windows allowing abundance of natural light, & high ceilings with recess lighting for a modern touch. This residence is one of the most popular floor plans offering direct access from garage to kitchen and living space with only 2 levels. Many builder upgrades includes: Stainless Steel GE Profile Refrigerator, KitchenAid built in oven, Whirlpool microwave, front load Whirlpool Washer & Dryer, Cherry stained cabinets, granite tile counter tops, tile floors, & cultured marble counter tops in bathrooms. Centrally located and great for commuters! Close to NEW Bart Station & Easy access to 680, 880, 237. Nearby tech companies: Tesla, Solar city, Cisco, SanDisk, KLA Tencor, Thermo-Fischer, Boston Scientific. Enjoy the playground, green belt, and club house w/ low HOA dues. Must See!
Courtesy: Realty Executives of Merced Mark Salcido
View additional info
Upstairs unit in Fremont Terrace! Cute 2 Bedroom 2 bath unit in a Great Location That is priced to sell! Very clean and well taken care of condo with Stainless steel appliances an laminate flooring througout! Walking distance to great schools and just a few min away to shopping, come see it today!
36055 Pizarro Fremont, CA 94536
Courtesy: Akimax Realty Kenrick Do
View additional info
Beautiful Home. Some features of property: Upgrade kitchens with quartz counter top, stainless appliances,tile floor, laminate floor, double paned windows, upgrade bathrooms,new interior & exterior paint. Excellent area.
50 Mowry Avenue Fremont, CA 94536
Courtesy: American Gateway Real Estate & Mercedes Albana
View additional info
Large lot of 29,840 SF. Special delight for a family with landscaping or gardening hobbies and a handyman/contractor. Multi family dwelling in as is condition purchase.
Courtesy: Key Legacy Real Estate Service Viviane Phan
View additional info
37703 2nd Street Fremont, CA 94536
Courtesy: Sereno Group Bev Harada
View additional info
This charming home is located in the historic Niles neighborhood. It boasts a beautiful garden with automatic sprinklers and drip system that cleverly waters the array of succulents, planted in re-purposed gutters along the fences. The updated kitchen has granite counters and stainless appliances. The bathroom is nicely updated, and there is a generously sized laundry room. Original hardwood floors in the living room adds to the charm. The unfinished basement provides storage, storage, storage! A newer deck and an automatic awning overhead are great for barbecues and lounging, plus enjoy a large patio area for your entertainment. This corner lot home is one block from shops and restaurants.
Courtesy: Mikasa Homes & Funding FongLing King
View additional info
UPDATED MEADOWBROOK CONDO in a desirable gated community! Spacious End-Unit located on the 3rd floor with vaulted ceiling for more natural light, a balcony and good views! Kitchen has tile counters, SS appliances, recessed lighting, range hood with carbon filters, laminate flooring, blinds, electric fireplace, washer & dryer, plus fresh paint throughout the interior. Close to Amtrak, Bart Station, nearby shopping, Quarry Lakes, schools, Hwy 880 & Dumbarton Bridge. Move-in Ready! Check for Open House. (Note: Home comes with 1 yr Home Warranty.)
434 D Street Fremont, CA 94536
Courtesy: Coldwell Banker Residential Br Lori Ferrito
View additional info
Fabulous! This is your chance to live in this highly desirable Niles neighborhood. This lovely home is bathed in natural sunlight featuring a separate family room, eat in kitchen, formal dinning, spacious living room and 3 good size bedrooms. Great yard that is made for entertaining or to just enjoy a quiet cup of coffee. Just around the corner to the award winning Niles Elementary School. Enjoy all that the Niles shopping district has to offer, shops, restaurants and best of all the Niles Canyon Train. Buyers check availability at local schools.
3816 Fiano Comm Fremont, CA 94555
Courtesy: Faira.com Corp Larisa Kramer Rahmil
View additional info
BEAUTIFUL DETACHED SINGLE FAMILY HOME! Newly Constructed, nestled in Private Community on a Premium lot in Highly Desirable Fremont Northgate neighborhood. Abundant Natural Light, Modern Design with Finest materials. Gourmet Kitchen-Stainless Steel Appliances. Conveniently located near I-880, Dumbarton Bridge, BART & ACE train stations. Downstairs Bedroom/Bath/Suite with separate entrance. Walking distance to Park, Ranch 99, Lucky, Restaurants. Highly rated Fremont unified schools. MUST SEE !
Courtesy: Silicon Valley Realty Network Paul Drozdiak
View additional info
This exquisitely remodeled Town Home is complete with newer stainless steel appliances, gorgeous cabinetry and new counter tops that will please the most discerning chef. You will be confident of the local schools as they are all Top Rated Schools and some of the highest rated in the Bay Area. With an expansive living area you can enjoy the luxurious feel of the California sunshine in the large and open west facing living room as it opens to your own private Balcony. The Master Bedroom affords both privacy and space and the large walk in closet provides sizable storage for the your convenience. Two full bathrooms with a walk-in shower in the downstairs bathroom provides all the facilities you will ever need. A large two car garage with internal access makes your arrival and departure from your new home in all conditions easy and comfortable and a luxurious pool and spa are just steps away. All your favorite shops along with BART are close by! Your new home awaits!!
408 Sequim Fremont, CA 94539
Courtesy: Intero Real Estate Services Ulysses S. Chua
View additional info
NICE & COZY CONDO in a Great Location With Awesome School Districts. IMMACULATE & MUST SEE UNIT with 2 Beds & 2 Baths Located in the 1st Floor. Kitchen has Granite Counter Tops & Updated Floor Tiles. Fantastic Living Room Boasts Laminate Wood Floors & unit has a balcony. Close to Commercial Districts & Easy Access to Freeways 680 & 880.
4903 Cody Court Fremont, CA 94538
Courtesy: Intero Real Estate Services Rebecca A. Gallardo
View additional info
Spacious home with in-law suite ~ can be used as a partial primary home with rental feature. Or enjoy the amenities of a large home with open floor plan with Living room, Dining Room, and Kitchen combination. Fireplace surround in dining room adds warmth and nice design. This 3 bedroom 2 bath home, with a pool and large corner lot offers so much to be desired. Your home ownership dreams await your tasteful decor. Swimming pool summer fun with deck and landscaped yards ~ direct access to bathroom from exterior for your entertaining delight. 2 car garage, side yard access and 2 large sheds for your storage needs. This home has a home security system with 8 cameras will stay. A must see!
37063 Towers Way Fremont, CA 94536
Courtesy: Faira.com Corp Larisa Kramer Rahmil
View additional info
New construction 2017 in well-established Fremont neighborhood. Never lived in TURN KEY! New upgraded appliances, Quartz Counters & kitchen cabinets. New tile & carpet throughout! New baths with large master bedroom suite. New electrical, plumbing, Sheetrock & built in wireless internet. New stucco, roof, garage door, tank less water heater & new forced air. New sod w/ sprinklers front & back & new fences! Near schools & shopping. Easy access to 680/880 & Dumbarton bridge! This is a MUST SEE!!
43943 Rosemere Drive Fremont, CA 94539
Courtesy: Keller Williams Realty-Silicon Chris Luu
View additional info
Welcome to the highly desirable lovely neighborhood of Cameron Hills in Fremont. This beautiful corner lot home features 4 bed 3 bath 2353 square feet with laminate flooring, stainless steel appliances, granite countertop, vaulted ceilings, Jack n Jill bathroom, wet bar and a hardscaped backyard with flagstone pavers. Conveniently located near all the top rated schools Mission Valley Elementary, Hopkins Junior High and Mission San Jose High.
33612 Pack Horse Street Fremont, CA 94555
Courtesy: KB Home Duc Hoang
View additional info
440 Via Vera Cruz Fremont, CA 94539
Courtesy: United Capital Investment Sandra Tang
View additional info
Mission San Jose Townhome. Updated and beautifully remodeled bathroom. Down stair bathroom remodeled with permit: New Counter, New Shower, and New Tile Floor. Granite kitchen counter. New stainless steel range hood. Newer appliances. Dual pane windows. Hardwood floor in Dining and Living Area. Master bedroom with large walk-in closet. Interior newly painted.
4421 Nicolet Avenue Fremont, CA 94536
Courtesy: Intero Real Estate Services Hugo De Hoyos
View additional info
Fabulous Tri-level home located in a desirable district in Fremont. Recently renovated. Brand new kitchen with lots of cabinet space and granite counters, new carpet and fresh paint. Formal family and living room, and a large lot in a desirable location...
Courtesy: Imperial Real Estate500 Josie Mongi
View additional info
Beautiful Studio home in one of the most desirable areas in Fremont. Updated kitchen with S/S appliances, maple cabinets/pantry, tile flooring in kitchen and bathroom, granite counter tops. Wall Bed/Hideaway bed. Laminate wood floor in livingroom and walk in closet. Quiet ground floor with private patio and extra storage room. Close to Bart, shopping, Fremont school disctrict. One block away from Quarry Lake. Complex has greenbelt, common laundry area, community pool, clubhouse and exercise/gym facility.
246 Grove Avenue Fremont, CA 94539
Courtesy: Realty World-SVI Group Mimi Du
View additional info
Open Houses Sat 1-3 pm. Sun 1-4 pm. Lovely Single Family Home, Excellent School District: Mission San Jose Elementary 10; William Hopkins Middle 10 & Mission San Jose High 10. 3 Bed/2 Bath with Large Backyard. Detached Garage. Hardwood Floor Throughout. Convenient Inside Laundry Room. Basement with Storage. Close to Shops, Restaurants, Park and Schools. Easy Access to 680. A Must See. Historical house.Needs TLC.
43678 Salem Way Fremont, CA 94538
Courtesy: Intero Real Estate Services Phil Waddingham
View additional info
Courtesy: Yamasaki Real Estate Services, Betsy Yamasaki
View additional info
$200K Drop from original Price!! Motivated Seller!! Don't Miss Out on seeing this Gorgeous, Single-Story Custom, Executive Home in the 24/7 Gated-Guarded Community of Avalon located in the Fremont Foothills. Upgraded with hardwood flooring, granite counters, marble master bath, 3 Beds/3.5 Baths - Extra bedroom used as Office. Huge Lot. Pool, Gazebo, Outdoor kitchen. Great School District - - - a MUST SEE!!! OH Sat 4/15/17 1-4p.m.
3060 Woodside Terrace Fremont, CA 94539
Courtesy: Yamasaki Real Estate Services, Betsy Yamasaki
View additional info
This Home is a MUST SEE! Absolutely Stunning in the 24/7 Gated/Guarded Community of Avalon in the Fremont Foothills. Brand New Kitchen February, 2016. Upgraded, 5 Beds/4.5 Baths, 3 Suites (one on first floor), Media Room, Huge Bonus Room, Office, 2-2-car Garages (inside access) - Awesome Backyard with pool/spa/outdoor kitchen with upscale appliances, two arbors, firepit, waterfall, bocce ball court and MORE!!! Sit at the granite counter or in a deck chair and enjoy the VIEWS!!! OH Sat 04/15/2017, 1-4 p.m. Give Guard address of house at Gate for Entrance!
Refine Property Search
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text:
Rankings are fluid as new names emerge, prospects are seen live, and new tape is evaluated.
Less than a decade ago, it was basically one class at a time. Then it became two, next it was three, and now select football prospects in middle school have received scholarship offers.
Recruiting analysts are now evaluating all high school prospects and rankings are coming out earlier and earlier. When looking at the best in the South, this feature throws out class, and just simply ranks the top 15 prospects as of right now.
1. Trevor Lawrence, 2018 QB, Cartersville (Ga.)
Lawrence is a premiere prospect and he has a great shot at starting No. 1 in the country when Scout ranks the 2018 class later this summer. He has everything you look for in a quarterback and his top schools are Clemson, Florida, Georgia, Oklahoma, and Tennessee. He could make a decision this summer.
2. Dylan Moses, 2017 ATH, Bradenton (Fla.) IMG Academy
Moses is from Louisiana, but will play his senior season in Florida. He is a one-time LSU commitment. He named Alabama, Texas, UCLA and USC along with LSU his top five schools earlier this spring. Moses has shined on both sides of the ball all his life, but he is all defense in college.
3. DeAngelo Gibbs, 2017 ATH, Loganville (Ga.) Grayson
Gibbs is a natural athlete who does so many things at a high level. He is most comfortable at wide receiver now, but he is a defensive back in college. He keeps things close to the vest when talking recruiting, but Alabama, Georgia, Oklahoma and Tennessee are some of the schools in the mix. He does not plan to commit until late in the process, after he takes his official visits.
4. Stanford Samuels III, 2017 CB, Pembroke Pines (Fla.) Flanagan
Samuels has everything you want in a shutdown cornerback. He may actually be a little low on this list at No. 4. He is a Florida State legacy, so the Seminoles are considered the leader. Clemson, Georgia and Michigan are also schools still under consideration.
5. Tedarrell Slaton, 2017 OG, Fort Lauderdale (Fla.) American Heritage
Slaton can be dominant in the trenches. He is extremely powerful and he really knows how to control the man in front of him. He looks like an NFL interior lineman now. Clemson is the school he named as his leader recently. Alabama, Florida State, Michigan and many others are still in this race.
6. Devonta Smith, 2017 WR, Amite (La.)
Smith is as smooth as they come. He is still a little light, but he will add weight and strength over time. He is explosive and he makes play after play look easy. He is being compared to Odell Beckham being in the state of Louisiana. Smith is a former Georgia commit, but Alabama is considered the team to beat now.
7. Tyreke Johnson, 2018 CB, Jacksonville (Fla.) Trinity Christian
Johnson has been a recruit known throughout the south since eighth grade. He has size you cannot coach, he is smooth in transition, and he is capable of being a big-time cornerback or safety on the next level. He really likes Stanford, UCLA and USC out west, and schools like Alabama, Florida and LSU closer to home.
8. Cam Akers, 2017 RB, Clinton (Miss.)
Akers plays quarterback in high school because the ball needs to be in his hands. He can throw, but his home with be at running back. He has a great blend of power, speed, and elusiveness out of the backfield. Akers was a one-time Alabama commit and the Crimson Tide is still in this, but Georgia, Mississippi, Ohio State and Tennessee are likely higher on his list.
9. VanDarius Cowan, 2017 LB, Palm Beach Gardens (Fla.)
Cowan is a two-time Florida State commit, but he is now committed to Alabama. Cowan is an elite linebacker who can run, hit and rush the passer. The biggest surprise when watching Cowan live this spring is how he moves in space and runs with running backs at his size. He has NFL written all over him.
10. Zamir White, 2018 RB, Laurinburg (N.C.) Scotland
White has been a workhorse for Scotland the last two seasons. He plays like a veteran and has handled the national attention as well as anyone. He is a strong, durable back being compared to former Georgia star, NFL Rookie of the Year, Todd Gurley. White has spent a lot of time on the road this spring and schools like Alabama, Florida State, Georgia and LSU. He is not planning to make a decision too early.
11. Xavier Thomas, 2018 DE, Florence (S.C.) Wilson
Thomas is a freak off the edge and he is a nightmare for opposing offensive tackles. If he does not win the battle with speed, then he can work inside moves and use power to get to the quarterback. His top four schools are Clemson, Florida State, Ohio State and Tennessee.
12. Owen Pappoe, 2019 LB, Loganville (Ga.) Grayson
Pappoe is only a freshman right now, but he is not your normal freshman. He is an elite athlete who started at Grayson, one of the top high school programs in the South last fall. He has great size for his age, he shows awareness on the field, he can run, and the scary thing is, he is still going to get so much better. Keep an eye on Tennessee early, but he is still eyeing plenty of schools including Florida State and Georgia to name a couple.
13. Calvin Ashley, 2017 OT, Orlando (Fla.) Dr. Phillips
Ashley has been a prospect high on Scout's board since he was a freshman. He is the lowest five star in 2017 out of the South, so he has a lot of eyes on him this summer and fall. He has very good size, he is a good bender and he has flashed good feet. He is currently committed to Auburn.
14. Jake Fromm, 2017 QB, Warner Robins (Ga.) Houston County
Fromm just continues to impress. He is a 2017 quarterback in the five star discussion. The Georgia commit was the Gatorade Player of the Year in 2015. He has a lightening quick release, a very live arm, and he is a natural leader.
15. Patrick Surtain, 2018 CB, Fort Lauderdale (Fla.) American Heritage
Surtain is a real contender for a five star ranking when Scout ranks the 2018 class in the coming months. He is impressive on the hoof, he knows the game, he has great range, and you cannot teach his instincts. He knows the game inside and out. It is early, but Alabama, Clemson, Florida, Florida State and LSU are his top schools.


text:
Platinum and/or palladium are the catalytic substances in our combustors. These precious metals are applied to the honeycomb of the combustor (either ceramic or steel).
Your wood stove or fireplace will need a bypass in order to use a catalytic combustor.
When the exhaust temperature isn't high enough for a combustor to "light-off" the wood-smoke exhaust must have an alternate route to go, until the combustor is hot enough to perform its function.
Canned Combustors
Original equipment catalytic combustors that are "canned" (the ceramic is wrapped with a stainless steel strip) should be replaced by new combustors that are also canned. Observe that canned combustors have gasket material sandwiched between the can and the ceramic honeycomb.
Woodstoves with canned combustors may, or may not, require additional gasket outside the can for a good fit inside the stove. This depends on the particular stove design. In some cases the combustor assembly consists of two or more pieces. Gasket should be used between the individual pieces for a good fit.
The Law:
Federal law requires any EPA-certified catalytic wood stoves be operated with combustors to reduce air pollution over the entire life of the stove.
How Much Gasket Do I Need?
To determine which gasket will work best, calculate the outer dimension of your combustor. Six-inch-round combustors require 19 linear inches of gasket. Seven-inch-round combustors require 22 linear inches of gasket. Oblong combustors require gasket twice the length, plus twice the width, PLUS two inches (a half-inch for each corner). Order CatGuard Gasket
More About Catalytic Combustors
Care And Replacement Manual For Woodstove Catalytic Combustors
Combustors are guaranteed to contain sufficient catalytic precious metals (platinum and/or palladium) formulated and properly applied to conform to requirements of the U.S. Environmental Protection Agency for certified wood-burning products, and when installed in USA are warranted for structural integrity for five years or 12,000 hours burn-time, whichever comes first, when correctly installed and maintained. Failure due to incorrect use, including with fuels other than dry natural wood, is excluded.
Manufacturer will replace a defective combustor, free of charge, for the first 90 days following the date of original purchase.
After the first 90 days, replacement will be at the following discount:
Year 1 — 50% of original price paid
Year 2 — 40% of original price paid
Year 3 — 30% of original price paid
Year 4 — 20% of original price paid
Year 5 — 10% of original price paid
Year 6 — Replacement at current retail price.
5-10 Business Days.
Product can not be shipped to Canada!
Our loyal customers have left 56 company testimonials giving us an average of 5/5 stars for great service. Would you like to leave your own feedback, testimonial, or review? Leave My Feedback
Very fast processing and shipping of my order. Would definitely order from them again!
donna h
Thank you!!! What great response!!! I appreciate the quickness and the service of your company!!!
Bad experience
I went by the description they had but apparently it was not the actual size of the product but the size of the fireplace. No where on the website do they have the diameters of the actual product. After getting the product I noticed it was the wrong size and tried returning it. They want 20% restocking and I pay for shipping due to the terms but in the terms and condition it also States you'll get a courtesy email to make sure you purchase the right product before it ship. Well after I said that they stop emailing me. CJs Hearth & Home Team The description on the web site said that it was a screen for a direct vent fireplace. After seeing a picture of your fireplace it was clear that you have a vent free fireplace not a direct vent. A direct vent fireplace has a sealed piece of glass on the front of the fireplace. This screen is made only for the Monessen models listed in the ...
Diane Spartanburg
Wish I would have found you sooner
Thanks for the help - I had stalled for years in doing this without a local company - this on-line process worked very well - clear directions and followup to questions and good suggestions. Thanks!
We received the fireplace doors in good shape the other day, they look great, beautifully made.
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Hosting migration
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A small girl sits on (I presume) her dad's shoulders on Patrick's Street. In the background is the Savoy Shopping Center, and of course the building side behind Dunnes is clearly visible in the scaffolding supports to the far left.
The houses and roofs of the North Side, Cork City.
This was taken from Carroll's Quay carpark almost a month ago. I love the old-style quality of the image, even if the shadow of the dish at the bottom detracts from that a bit.
Check out Stabarinde's Christmas in Cork shots. I'm glad my photos inspire you! There are a few gorgeous night shots in that set.
Cables from a crane on the construction site at the old Guy & Company building in Cork.
Christmas decorations on Paul Street, Cork a few days before Christmas Day last year.
Hand held, and I hoped to get a "normal" straight shot but it's hard to crouch down while wearing a jacket and pushing a buggy so it's lopsided. I prefer it that way!
Conversion to b/w I think brings out the descending pattern of the lights along the street although the original image wasn't that saturated with colour to begin with.
During the Christmas rush I went searching for gifts for my wife and the first place I went was Susan's Fine Silver on Marlboro Street in Cork.
This is Susan's husband Barry, and that's Michael replacing merchandise in the window. Both were very helpful and I got two lovely gifts for Jacinta. Thanks for your help!
We popped in there this afternoon because I couldn't remember Barry and Michael's names. Lucky we did because Barry hadn't told his wife that I was posting this picture.
Latest news on the lens is that they're waiting on a piece from Sigma Japan. So much for it being fixed and in transit. That's almost 2 months to fix my lens.
A holiday caravan near a beach on the Dingle Peninsula.
This caravan has been in the same spot for at least the last 2 years, and probably longer. I wanted to shoot it for quite a while but it wasn't until I converted this shot to b/w that I was happy with it.
Oh, delicious spam! How I love you so! I want to spread you on sandwiches and lick the knife clean! Oh Yum!
Or not. I've never tasted the edible variety of spam, but like everyone else online I've had my fair share of spam emails, comments and blogs.
This is the real spam, made by Hormel. I spotted it in a store in San Francisco and snapped a quick shot of the aisle. Unfortunately the light was bad but, if you've never come across it before, it's a novelty worth posting, right?
Current spam stats:
1. Inphotos: 8510
2. Holy Shmoly!: 21689
3. Gmail: 2176
Spam is here to stay.
Cork City Fire Brigade were called out this evening to a shop on Patrick Street.
I'm not sure what the emergency was but two fire engines were called to the scene and had a Garda escort on the way there. Maybe a gas leak?
A huge crowd gathered around and when some people saw my camera they just had to pose for a shot .. more on that next week!
Trivia: these are my first shots posted in 2008 that was taken in 2008. Plenty more 2007 ones to go through yet though!
The Christmas rush is over. Sales have been bad for retailers as people suddenly realised, "Oh! The mortgage has to be paid!"
Anyway, I'm half-hoping I'll be able to pick up a Nintendo Wii somewhere. I don't suppose they'll be on sale will they?
I celebrate 10 years online this year! Go me!
Check out Donal's new photoblog, Pana Vision!


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In light of the news about the updated construction process for the new MacBooks, it is high time you got a brief edumication on the history of unibody construction. It may seem revolutionary, but the method Apple is using derives from the early 20th century monocoque ("single shell") technique of using an object's external skin to support structural loads. It has its roots in the airline industry where a price drop in aluminum in the 1920's made it affordable to meet the demand for stiff, strong, smooth skins that could handle the stress of high altitudes and increasingly powerful aircraft. By the end of WWII, almost all high-performance aircraft were built using monocoque or semi-monocoque technique.
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Kinja is in read-only mode. We are working to restore service.


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Super-Cute 1-Bdrm on Intracoastal Waterway! Priced for Minimum 1-Month Stay(9 Reviews)
The owner has been emailed.
To book this property, you need to complete your booking through checkout on the VRBO website. We can't protect your payment if we don't know you've booked through us. Learn more
- Minimum stay:21-27 nights
- Sleeps:2
- Bedrooms:1
- Bathrooms:1
- Property type:Condo
- Internet:Yes
- Pets considered:No
- Wheel chair accessible:No
About the Property
Old Florida charm on Boca Ciega, 5-min bike ride to Gulf beaches.
Great care was taken in this remodel project to maintain the character and style of mid-century design. Compact European appliances with clean-line cabinetry makes this space very live-able.
Very close to Corey Avenue Historical District with dozens of shops, restaurants, and bars within easy walking distance. Bus line and beach trolley stop is 1/2 block away. Beach is about 15-min walk away. Older boat slip fits up to a 25-footer (additional $95/mo for slip - no electricity to slip).
Isle of the Bay was built in the mid 1950's and caters mostly to snowbirds and early retirees. It's quiet and sunny. Perfect for active folks with a sense of nostalgia for vintage character and style in a new remodel. No hand-me-down furniture in this VRBO!
Includes use of 2 beach-cruiser bikes (one-speed, coaster brakes - use at your own risk).
Strictly no-smoking even in the common areas. Sorry no pets.
NOTE - 1-month minimum, please.
Member since: 2014
Property Type
- Condo
- On floor 2
- 435 sq. ft.
House Rules
- Max. occupancy 2
- Non Smoking Only
Accommodation Type
- Vacation Rental
- Guests Provide Their Own Meals
Onsite Services
- Housekeeper Optional
- Long-Term Renters Welcome
- Wheelchair Inaccessible
Bedrooms: 1 Bedroom, Sleeps 2, Beds for 2
Bedroom 1: 1 king
Can be setup as Twin Beds (adult-length) with notice...moreless
- Large bedroom with small walk-in closet with a custom, maple closet organizer that makes excellent use of the space. Bed is a king, but can be separate into two twins (extra long length). Great light and breeze through large window. 42' LED TV...moreless
Bathrooms: 1 Bathroom
Other Amenities
- Quiet complex of about 24-units, mostly early retirees/snowbirds.
- Away From It All
- Botanical Garden
- Festivals
- Library
- Live Theater
- Marina
- Museums
- Recreation Center
- Restaurants
Local Services & Businesses
- ATM/Bank
- Fitness Center
- Groceries
- Hospital
- Laundromat
- Medical Services
Leisure Activities
- Antiquing
- Beachcombing
- Bird Watching
- Boating
- Outlet Shopping
- Shelling
- Shopping
- Sight Seeing
- Walking
- Wildlife Viewing
Location Type
- Water View
- Waterfront
Sports & Adventure Activities
- Cycling
- Deepsea Fishing
- Golf
- Jet Skiing
- Kayaking
- Paragliding
- Parasailing
- Pier Fishing
- Sailing
- Snorkeling/Diving
- Sound/Bay Fishing
- Swimming
Air Conditioning:
New high-efficiency ductless heat-pump system installed Sept 2014 with separate zones for bedroom and living room. Very quiet and effective...moreless
- Clothes Dryer:
Fitness Room / Equipment:
Property does not have its own fitness room, but the St Pete Beach Community center is 100-yards away with 3 treadmills, 3 recumbent bikes, 2 elliptical bikes, weights, etc. Cost is $2/day or $16/m...moreless
- Hair Dryer
- Heating:
- Internet:
- Iron & Board
- Linens Provided:
Coffee Maker:
Kuerig pod style. We provide a starter-pack of 10 pods so you don't have to run out immediately...moreless
Dishes & Utensils:
Full compliment of cooking items. We enjoy cooking and just hate when places have cheap goods. ...moreless
Compact kitchen with brand new appliances. Euro-feel with great storage. (NOTE - no dishwasher - with so many nearby restaurants, cooking is uncommon for most)...moreless
- Microwave:
- Oven:
Pantry Items:
Some spices and basic items; starter-pack for coffee (sugar, etc). Our goal is to let you relax immediately. ...moreless
2-beach cruiser bikes (1-speed, coaster brakes) free for guests. Use at your own risk. Bring back in same condition as you borrowed. ...moreless
House Cleaning
- Housekeeper Optional
RatesCurrency Conversion
|Weekend Night
|Monthly *
2016-2017 Winter Escape
Nov 5 - May 6, 2017
27 night min stay
2017 Summer
May 7 - Oct 13, 2017
21 night min stay
2017-2018 Winter
Oct 14 - May 6, 2018
21 night min stay
My Standard Rate
27 night min stay
Additional information about rental rates
- Refundable Damage Deposit $500
- Tax Rate 13%
Owner's cancellation policy:
50% refund for cancellations more than 30 days before check-in date.
100% refund for cancellations more than 60 days before check-in date.
Don't forget your vacation protection! Get protected now
Adding our Vacation Protection services can make sure your getaway goes smoothly, no matter what. We offer Cancellation Protection and Damage Protection so you can truly relax.
- Restaurant
- 1.4 miles to rental
- View on map
- Restaurant
- 2.4 miles to rental
- View on map
- Restaurant
- 1.6 miles to rental
- View on map
- Restaurant
- 2.3 miles to rental
- View on map
- Restaurant
- 4.0 miles to rental
- View on map
- Restaurant
- 0.1 miles to rental
- View on map
12-miles to Clearwater Beach (2013 best-beach)
15-miles to Ft Desoto Beach State Park (2005 #1 beach)
45-miles to Busch Gardens
80-miles to Legoland
90-miles to Disney World
100-miles to Universal Studios
Owner info
Year Purchased: 2013
About the owner: We moved to Florida from San Francisco several years ago. We love travel, discovery, and adventure: Florida has fed our thirst for all. We live near downtown St Petersburg and enjoy sharing the area with friends and family. I guess we are nostalgic at heart and appreciate the character that comes with a vintage patina - some things just seem to get better with age!
Why the Owner Chose Corey Ave, Saint Pete Beach, FL, USA:
I have to confess, the boat slip. Cheryll likes to tell people 'Pete bought a slip with a condo attached to it.' True, but we both fell in love with this complex - the light that streams into the windows is amazing; the neighborhood is so very convenient; and our neighbors (including many seasonal guests from around the world) are really nice. It's a quaint place that really tugs at our nostalgic hearts. We had a great time renovating the property, and tried to honor the period in which it was built. While working on the remodel, I often thought of my long-passed grandfather who, starting in the 1960's, traveled from his New Jersey home to Florida where he loved walking, being outside, and fishing (he would have loved the fishing pier this place has). For us, vintage places just seem to build memories with more depth and emotion. We find ourselves smiling frequently when we're at this apartment and we hope this 'Old Florida efficiency' condo evokes a similar emotion with you.
The Unique Benefits at this Condo:
Nearby shopping, restaurants, and bars. Grocery store is less than a block away - no need for a car every day. Corey Avenue, the down-town of St Pete Beach, is about 2-1/2 blocks away where you'll find shopping and a weekly street-market (farmers market and craft market).
Contact us
Send email
Links to more information:
- Click Here to see All My Rental Listings
Living Room - 42' LCD TV; leather club chairs.
Kitchen - quartz counters, new appliance
View from just outside the front door. Pool and intercoastal is across street.
King bed (can be split into twins upon request). Beautiful light. New A/C.
42' LED TV in Bedroom - and hardwood custom closet system in walk-in closet
Remodeled shower with glass enclosure
2 Beach Cruisers. 1-gear, coaster brakes. Free use (at your own risk)
Blue Herron awaiting dinner down at the docks.
Corey Avenue Saturday Market. 5-min walk (3-blocks)
Boat slip (empty). 30-ft sailboat in next slip. Fishing off dock. No electricity
Sunset from balcony, overlooking the pool.
Large swimming pool right on intercoastal (note: pool is not heated)
Kitchen area with compact Euro-appliances (large grocery store 1/2 block away)
435sf 1-bedroom efficiency. Excellent use of space with nice furnishings
Beds setup as twins (XL Mattresses).
Concrete counter and porcelain tile in bathroom
Bathroom was completely renovated. Concrete counter and Italian porcelain tile.
Second floor unit, built in the mid 1950's
Fishing off the dock in March. She caught a 7-lb sheep's head this trip!
Sunset at Bongos Beach Bar - about a mile away.
Green Thumb Festival - April. About 15-min drive from Condo
Bongo's Beach Bar & Grill - about 1-mile from the condo
This listing was first published here in 2014.
Date last modified - Sunday, April 23, 2017
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Work Exchange Application
Dear Applicant,
Our staff is here to serve our members, and work exchangers are here to support the staff and serve the members as needed. Enhancing the environment that has been created requires an atmosphere of harmony, discipline, right-mindedness and a yearning for spiritual growth. To be on work exchange requires a combination of fulfilling the needs of our members, offering excellence in your efforts, appreciating and respecting others who are serving, and, at the same time, effort in pursuing the opportunity to advance your own spiritual growth.
Our retreat center is rooted in the teachings of Paramahansa Yogananda, author of Autobiography of a Yogi and the founder of the Self-Realization Fellowship. We honor all truth seekers. Our meals are vegetarian. Song of the Morning has a strict policy: the use of tobacco, alcohol, illegal drugs and coming onto the property under the influence are prohibited.
Are you ready for an important step in the unfoldment of your life? If so, please complete and submit this application. We will be in contact with you soon.


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Topik Kuliah: Microbial Biotechnology 1. 2. Bioteknologi; definisi dan sejarahnya &Teknologi DNA Rekombinan Bioremediation and biomass utilization 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Ethanol Microbial cell fuel Bioplastics produced by microorganisms Probiotics, Prebiotics and Synbiotics Biocatalysis /biosensor Molecular diagnostics Vaccines and therapeutics agents Plant-growth promoting bacteria Microbial insecticides Microbial synthesis of commercial products Large-scale production of proteins from recombinant IRM ATW microorganisms 14. Regulasi & paten microb produk bioteknologi Bacaan : Glazer AN & Nikaido H. 2007. Microbial Biotechnology, Fundamentals of Applied Microbiology, 2 nd Edition. Cambridge University Press. Cambridge. Microbial Biotechnology Biotechnology: Definition & History Recombinant DNA Technology 1
2 What is biotechnology? Biotechnology = bios (life) + logos (study of or essence) – Literally 'the study of tools from living things' CLASSIC: The word "biotechnology" was first used in 1917 to describe processes using living organisms to make a product or run a process, such as industrial fermentations. (Robert Bud, The Uses of Life: A History of Biotechnology) LAYMAN: Biotechnology began when humans began to plant their own crops, domesticate animals, ferment juice into wine, make cheese, and leaven bread (AccesExcellence) What is biotechnology? GENENTECH: Biotechnology is the process of harnessing 'nature's own' biochemical tools to make possible new products and processes and provide solutions to society's ills (G. Kirk Raab, Former President and CEO of Genentech) WEBSTER'S: The aspect of technology concerned with the application of living organisms to meet the needs of man. WALL STREET: Biotechnology is the application of genetic engineering and DNA technology to produce therapeutic and medical diagnostic products and processes. Biotech companies have one thing in common - the use of genetic engineering and manipulation of organisms at a molecular level.
3 What is biotechnology? Using scientific methods with organisms to produce new products or new forms of organisms Anytechniquethatuseslivingorganismsor substances from those organisms or substances from those organisms to make or modify a product, to improveplantsoranimals,ortodevelop microorganisms for specific uses What is biotechnology? Biotechnology is a multidisciplinarian in nature, involving input from Engineering Computer Science Cell and Molecular Biology Microbiology Genetics Physiology Biochemistry Immunology Virology Recombinant DNA Technology Genetic manipulation of bacteria, viruses, fungi, plants and animals, often for the development of specific products
4 What are the stages of biotechnology? Ancient Biotechnology early history as related to food and shelter, including domestication Classical Biotechnology built on ancient biotechnology fermentation promoted food production medicine Modern Biotechnology manipulates genetic information in organism genetic engineering Ancient biotechnology Paleolithic society – Hunter-gatherers Nomadic lifestyle due to migratory animals and edible plant distribution (wild wheat and barley) (~2 x 10 6 yrs.) Followed by domestication of plants and animals (artificial selection) People settled, sedentary lifestyles evolved (~10,000 yrs. ago) Cultivation of wheat, barley and rye (seed collections) Sheep and goats milk, cheese, button and meat Grinding stones for food preparation New technology Origins of Biotechnology Agrarian Societies History of domestication and agriculture
5 Long history of fermented foods since people began to settle (9000 BC) (fervere –to boil) Often discovered by accident! Improved flavor and texture Deliberate contamination with bacteria or fungi (molds) Examples: Bread Yogurt Sour cream Cheese Wine Beer Sauerkraut Ancient biotechnology Fermented foods and beverages Dough not baked immediately would undergo spontaneous fermentation would rise Uncooked fermented dough could be used to ferment a new batch no longer reliant on "chance fermentation" 1866 – Louis Pasteur published his findings on the direct link between yeast and sugars CO 2 + ethanol (anaerobic process) 1915 – Production of baker's yeast – Saccharomyces cerevisiae Ancient biotechnology Fermented foods and beverages
6 Different types of beer Vinegar Glycerol Acetone Butanol Lactic acid Citric acid Antibiotics – WWII (Bioreactor developed for large scale production, e.g. penicilin made by fermentation of penicillium) Today many different antibiotics are produced by microorganisms Cephalosporins, bacitracin, neomycin, tetracycline……..) Classical biotechnology Industry today exploits early discoveries of the fermentation process for production of huge numbers of products Substrate + Microbial Enzyme Product Examples: Cholesterol Steroids (cortisone, estrogen, progesterone) (hydroxylation reaction -OH group added to cholesterol ring) Classical biotechnology Chemical transformations to produce therapeutic products
7 Amino acids to improve food taste, quality or preservation Enzymes (cellulase, collagenase, diastase, glucose isomerase, invertase, lipase, pectinase, protease) Vitamins Pigments Classical biotechnology Microbial synthesis of other commercially valuable products Cell biology Structure, organization and reproduction Biochemistry Synthesis of organic compounds Cell extracts for fermentation (enzymes versus whole cells) Genetics Resurrection of Gregor Mendel's findings 1866 1900s Theory of Inheritance (ratios dependent on traits of parents) Theory of Transmission factors W.H. Sutton – 1902 Chromosomes = inheritance factors T.H. Morgan – Drosophila melanogaster Modern biotechnology
8 Molecular Biology Beadle and Tatum (Neurospora crassa) One gene, one enzyme hypothesis Charles Yanofsky colinearity between mutations in genes and amino acid sequence (E. coli) Genes determine structure of proteins Hershey and Chase – 1952 T2 bacteriophage – 32 P DNA, not 35 S protein is the material that encodes genetic information Modern biotechnology Watson, Crick, Franklin and Wilkins (1953) X-ray crystallography 1962 – Nobel Prize awarded to three men Chargaff – DNA base ratios Structural model of DNA developed DNA Revolution – Promise and Controversy!!! Scientific foundation of modern biotechnology based on knowledge of DNA, its replication, repair and use of enzymes to carry out in vitro splicing DNA fragments Modern biotechnology
DNA RNA Protein TranscriptionTranslation Genetic code determined for all 20 amino acids by Marshal Nirenberg and Heinrich Matthaei and Gobind Khorana – Nobel Prize – 1968 3 base sequence = codon 9 Modern biotechnology Breaking the Genetic Code – Finding the Central Dogma An "RNA Club" organized by George Gamow (1954) assembled to determine the role of RNA in protein synthesis Vernon Ingram's research on sickle cell anemia (1956) tied together inheritable diseases with protein structure Link made between amino acids and DNA Radioactive tagging experiments demonstrate intermediate between DNA and protein = RNA RNA movement tracked from nucleus to cytoplasm site of protein synthesis Modern biotechnology
10 What are the areas of biotechnology? Organismic biotechnology uses intact organisms and does not alter genetic material Molecular Biotechnology alters genetic makeup to achieve specific goals Transgenic organism: an organism with artificially altered genetic material Recombinant DNA Recombinant DNA is a molecule that combines DNA from two sources Also known as gene cloning Creates a new combination of genetic material Human gene for insulin was placed in bacteria The bacteria are recombinant organisms and produce insulin in large quantities for diabetics Genetically modified organisms are possible because of the universal nature of the genetic code
11 Basic Cloning Process Plasmid is cut open with a restriction enzyme that leaves an overhang: a sticky end Foreign DNA is cut with the same enzyme. The two DNAs are mixed. The sticky ends anneal together, and DNA ligase joins them into one recombinant molecule. The recombinant plasmids are transformed into E. coli using heat plus calcium chloride. Cells carrying the plasmid are selected by adding an antibiotic: the plasmid carries a gene for antibiotic resistance. Recombinant DNA methods – Restriction enzymes Enzymes from bacteria Used to cut DNA molecules in specific places Enable researchers to cut DNA into manageable segments – Vector molecule carrier of DNA fragment into cell – Transformation: uptake of foreign DNA into cells
Type IType IIType III Functions Endonuclease & methylase Endonuclease Conditions 2+ ATP, Mb 2+ Mg 2+ ATP, Mg Recognition sequences EcoK: AACN 6 GTGC EcoB: TGAN 8 TGCT Palindromic EcoP1: AGACC EcoP15: CAGCAG Cutting sitesAt least 1000bp away At or close to recog. seq 24-26 bp away 12 Restriction endonucleases – recognize specific nucleotide sequences, and cleave DNA creating DNA fragments. Each restriction endonuclease has a specific recognition sequence and can cut DNA from any source into fragments. Because of complementarity, single-stranded ends can pair with each other. – sticky ends » fragments joined together with DNA ligase Types of Restriction endonuclease
5' GAATTC 3' 3' CTTAAG 5' e.g. EcoRI site: Recognition sequences Recognize 4-8 bp palindromic sequences. Most commonly used enzymes recognize 6 bp which occurs at a rate of 4 6 =4096 bp. (4 4 =256 bp; 4 8 =65536 bp) 5'-CCCGGG-3' 3'-GGGCCC-5' p -GGG-3' OH-CCC-5' 5'-CCC-OH + 3'-GGG- p SmaI blunt ends 13 Restriction enzymes 1. Highly specific 2. Commercially available 3. Require Mg2+ for enzymatic activity 4. Compatible ends from different enzymes, Restriction sequences Cohesive/sticky ends
14 - ve electrode + ve electrode Restriction digestion Agarose gel electrophoresis Agarose: a polysaccharide derived from seaweed, which forms a solid gel when dissolved in aqueous solution (0.5%-2%)
15 Agarose gel electrophoresis Creating Recombinant DNA Molecules Cut DNA from donor and recipient with the same restriction enzymes Cut DNA fragment is combined with a vector Vector DNA moves and copies DNA fragment of interest Vector cut with restriction enzymes The complementary ends of the DNAs bind and ligase enzyme reattaches the sugar-phosphate backbone of the DNA
16 Covalently join the DNA molecules with the base-pairing cohesive ends, or blunt ends, if the 5'-ends have phosphate groups. DNA ligation Recombinant DNA molecules
Restriction Endonucleases Cloning Vector Types For different sizes of DNA: –––––––– plasmids: up to 5 kb phage lambda (λ) vectors: up to 50 kb BAC (bacterial artificial chromosome): 300 kb YAC (yeast artificial chromosome): 2000 kb Expression vectors: make RNA and protein from the inserted DNA – shuttle vectors : can grow in two different species 17
18 Plasmid Vectors To replicate, a plasmid must be circular, and it must contain a replicon, a DNA sequence that DNA polymerase will bind to and initiate replication. Also called "ori" (origin of replication). – Replicons are usually species-specific. – Some replicons allow many copies of the plasmid in a cell, while others limit the copy number or one or two. Plasmid cloning vectors must also carry a selectable marker : drug resistance. Transformation is inefficient, so bacteria that aren't transformed must be killed. Most cloning vectors have a multiple cloning site, a short region of DNA containing many restriction sites close together (also called a polylinker). This allows many different restriction enzymes to be used. Most cloning vectors use a system for detecting the presence of a recombinant insert, usually the blue/white beta-galactosidase system. What are the benefits of biotechnology? Medicine human veterinary biopharming Environment Agriculture Food products Industry and manufacturing
19 What are the applications of biotechnology? Production of new and improved crops/foods, industrial chemicals, pharmaceuticals and livestock Diagnostics for detecting genetic diseases Gene therapy (e.g. ADA, CF) Vaccine development (recombinant vaccines) Environmental restoration Protection of endangered species Conservation biology Bioremediation Forensic applications Food processing (cheese, beer) Monoclonal Antibodies Cell Culture Genetic Engineering Anti-cancer drugs Diagnostics Culture of plants from single cells Transfer of new genes into animal organisms specific DNA probes Localisation of genetic disorders Tracers Synthesis of Gene therapy Cloning Mass prodn. of human proteins Resource bank for rare human chemicals New antibiotics Synthesis of new proteins New types of plants and animals New types of food DNA technology Crime solving Molecular Biology Banks of DNA, RNA and proteins Complete map of the human genome
20 Agricultural Applications Ti plasmid has been early successful vector. – nitrogen fixation introduce genes that allow crops to fix nitrogen – reduce need for fertilizer – herbicide resistance insert genes encoding for proteins making crops resistant to herbicide – widespread herbicide use possible Agricultural Applications Insect resistance insert genes encoding proteins harmful to insects Real promise - produce genetically modified plants with traits benefiting consumers – iron deficiency in developing countries transgenic rice – increasing milk production bovine somatotropin
21 Transgenic rice "Golden rice" shown intermixed with white rice contain high concentrations of beta-carotene Transgenic Rice
22 Bovine Somatotropin Applications of Recombinant DNA Recombinant DNA is used to: Study the biochemical properties or genetic pathways of that protein Mass produce a particular protein (e.g., insulin) Sometimes conventional methods are still the better choice Textile industry can produce the dye indigo in E. coli by genetically modifying genes of the glucose pathway and introducing genes from another bacterial species
24 Benefits of Biotechnology: 1. Provide opportunities to accurately diagnose and prevent or cure a wide range of infectious and genetic diseases. 2. Significantly increase crop yields by creating plants that are resistant to insect predation, fungal and viral diseases, and environmental stresses such as short-term drought and excessive heat. 3. Develop microorganisms that will produce chemical, antibiotics, polymers, amino acids, enzymes, and various food addiitives. 4. Develop livestock and other animal that have enhanced genetically determined attibutes. 5. Facilitate the removal of pollutants and waste materials from the environment.
25 Social Concerns and Consequences 1. 2. 3. 4. 5. 6. 7. 8. 9. Will some genetically engineered organisms be harmful either to other organisms or to the environment? Will the development and use of genetically engineered organisms reduce natural genetic diversity? Should humans be genetically engineered? Will diagnostic procedures undermine individual privacy? Will financial support for molecular biotechnology constraint the development of other important technologies? Will the emphasis on commercial success mean that benefits of molecular biotechnology will be available only to wealthy nations? Will agricultural biotechnology undermine traditional farming practices? Will medical therapies based on molecular biotechnology supersede equally effective traditional treatments? Will the quest for patent inhibit the free exchange of ideas among research scientists?
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