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The 19th annual Twin Cities Jazz Festival will be headlined this year by several legendary jazz artists including John Coltrane Quartet pianist McCoy Tyner; Grammy Award-winning trumpeter Terence Blanchard; Anat Cohen with Choro Aventuroso; and keyboardist, organist, and Minnesota-native Bobby Lyle. The announcement was made this week by the festival's executive director, Steve Heckler. "I'm excited to have a jazz legend like McCoy Tyner and celebrated veterans like Terence Blanchard and Bobby Lyle perform alongside rising stars like Anat Cohen," said Heckler. "Thanks to the support we get from the community, we're able to keep our event free and open to all."
The festival, set for June 22-24 in and around Mears Park in Saint Paul's Lowertown arts district, will include headline acts on the Mears Park Main Stage, 6th Street Stage, and Union Depot Stage as well as performances at over 20 clubs, bars and studio venues.
Jazz 88
1555 James Avenue North, Minneapolis, MN 55411
Business Phone: 612.668.1735 | Studio Phone: 612.529.5236
Email: email@example.com


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Can a vegetarian person get a good meal at this restaurant?
Is this restaurant appropriate for Kids?
Does this restaurant offer delivery?
Is this an Italian restaurant?
Is this a pizza restaurant?
Are the prices at this restaurant mid-range / moderate?
Is this restaurant good for dining on a budget?
Is this restaurant wheelchair accessible?
Is this restaurant family-friendly?
Is this restaurant good for brunch?
Thanks for helping!
Pizzas are good, with plenty of filling, timely service and good pricing. A very fast paced place but usually pretty friendly guys!
Approached this store to help with pizzas to cheer up our fantastic local bus company who had armed defenders at their premises with guns blazing. They matched what I was willing to spend so the bus drivers had an extra lovely feast. Thanks again for your generous spirit and keep making yummy pizzas. I have no hesitation in recommending this...
Was most pleased with a new store and better pizza always rated it as low now with a change of heart well done guys just get the service right and its a winner
Own or manage this property? Claim your listing for free to respond to reviews, update your profile and much more.

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AJourney into Study of Astronomy & Science Not Limited To Only Exceptional Students. 2 NATIONAL ASTRONOMY & SCIENCE OLYMPIAD "Explore NASA with NASO" 2016-17
What is NASO…… National Astronomy & Science Olympiad is a platform that opens the door of limitless knowledge about the Astronomy and science explorations, for the students of Grade II to Grade X and to encourage talented young minds with amazing prizes who excel in the Olympiad in different programs including workshops, quizzes and many other informative & innovative sessions handled by eminent personalities in the field of space science, it's much about inspiring the young aspirants to dream bigger and to channel their interests in space science into the right path where they really belong. Mission: "To bring awareness and Interest to Children Towards the Field of Astronomy & Science and make their way to choose their career towards it." National Astronomy & Science Olympiad is one of the highly emerging concepts amongst young generation Olympiad. It is one of the unique and innovative platforms, and also serves an opportunity to the students by providing them the exposure to Science & Astronomy and a chance to visit NASA (Kennedy Space Center, Houston). It is scientifically designed and practically matured Exam which is divided into Two Rounds. 4
Why NASO…. The competition is not limited to the confined box of school curriculums. We move beyond the school curriculum, taking the participants to unexplored fields of knowledge, providing opportunities to meet legends of the field of space science, planetarium shows and much more. NASO is all about learning and gaining knowledge. NASO comes up with a concept called " Explore NASA with NASO". Where Children who stands first in competition gets an opportunity to go to " Kennedy Space Station( Houston). Runners with get a Chance to Win Scientific Education Trip to Singapore. All Participants will be awarded with International Participation Certificates. 5 Note: ISO 9000:2008 Organization. Note: ISO 9000:2008 Organization.
Eligibility : Students studying in Grades II to X following CBSE/ICSE/IGCSE/State Board. Syllabus: Relevant class syllabus. Subjects: Test of Science, Astronomy & Modern Science Pattern: Grade II to Grade IV (Science or Environmental Science with 50MCQ's in 60 Min) Grade V to Grade X (Science or Astronomy with 50MCQ's in 60 Min) 6 Level 1: School Round: First Round is to Test Ground & Basic Knowledge of a child in field of Astronomy, This Round comprises of 50 multiple choice questions with One Hour Time limit. Participation certificates and Merit Certificates will be given to all Participated Students. Gold, Silver & Bronze medals will be given to merit students. Principal & coordinators will be rewarded with special rewards. Grade II to IV will be tested purely in Subject of Science and Grades V to X will be tested in Subject of Astronomy & Science. Level 2: National Round: This Level is to Test Their Research Knowledge, which consists of 20 Research and Theme based questions with One Hour Time limit. Participation certificates and Merit Certificates will be given to all Participated Students. Gold, Silver & Bronze medals will be given to merit students. Toppers of this competition will be given announced prizes. MODE OF COMPETITION
Benefits of NASO…. 7 1.Identify their academic strengths and weaknesses. 2.Monitor and compare their year on year achievement with other students. 3.Gain valuable experience in taking international assessment tests. 4. Exploring in Astro Science. 1.Understand and analyse their student's academic strengths & weaknesses. 2.Collaborate with schools for remediation & skill development. 3.Design and plan meaningful educational activities for the student. 4. Get to know school performance in this competitive world. 1.Monitor and compare students' performance within the school, region and internationally 2.Discover students' latent potential and identify growth areas 3.Modify classroom activities and develop appropriate learning programs STUDENTS SCHOOL/TEACHER PARENTS
AWARDS & REWARDS 8 1 St Prize (7 Days NASA Trip ) 2 nd Prize (7 Days Science Trip in Singapore ) 3rd Prize (7 Days Science Trip In India )
How To Apply 9 NASO is organized only through the participating schools. The exam is conducted in the respective school of the participants during the school hours itself. The " NAVARS EDUTECH " will register the school and the students. Thereafter, the attendance sheet containing Roll Numbers of the students will be sent along with Question Papers and Guidelines for conducting the Examination. The schools are requested to send the details of the applicants in the prescribed Registration Form, Along with the Fees well before the last date to the NAVARS EDUTECH, 1-10-28-231/31, Second Floor, Road No 3, Indra nagar Colony, Nagarjuna Nagar, Hyderabad-500062 (Ph. 0065452525, 8885543354). Though not compulsory, the information asked for vide registration form may even be forwarded by mail at Registration form may even be downloaded from website. Last For Exam : October 30 th. Exam Centre : Your school itself. Exam Fee : First Round 120/- per student. [INR 20 should be retained by school]
10 NASO CITY WIDE PRESENCE IN 2015-16 CITIES WE ENTERD : Hyderabad,Vijayawada,Guntur,Vizag, Nellore,Thiruathi,Warangal,Kakinada, Rajamundry,Karimnagar,Nizambad, Bangalore,Lucknow,Pune,Mumbai, Chennai,Mysore,Coimbatore, Coachin,Ernakulam,Bhubaneshwar, Kolkatta,Raipur,Gulbarga,Chitradurga. Nanded. STATISTICS: Schools Covered : 463 Students Participated : 38700. States Enrolled : 9 Cities Covered : 26.
11 STATISTICS: Schools Coverage : 2000+ Students Participation : 1,00,000.+ States To Be Enrolled : 20 Cities to Be Covered : 180 TARGET FOR 2016-17 Entry in Middle East Tap schools in Oman,Qatar,Saudi Arabia,Jordan,Yemen,Ba hrain,UAE & Turkey.
Schools Participated- State Wise. 12 HYDERABAD Krishnaveni Group 100+ schools. Kakathiya Group 30+ Schools. Delhi Public School. Geetanjali Group. St.Patrics School. St.Michals School. Nagarjuna Group. Narayana Group. Sri Chaitanya Schools. Indus Universal School. Serenity Group. Podar Interntional-Pune Vibjior School-Mumbai City Montessori group- Lucknow. PSBB Group- Bangalore Rastriya Academy- Hiriyur. Dream India Group- Raipur. New Era Progressive School- Korba. Oxford EM School- Nanded. Cambridge School- Nanded. VIZAG & VIJAYAWADA Vignan Group. Vishaka Valley School. Rama Krishna School. Timpany School. Sri Prakash Group. Chinmaya Vidyalaya. J Sikle Academy. Shiridi Sai Vidyanikethan Siddhartha School. Delhi Public School. Future Kids School. TRIPS International. Vidhyanjali School. Green Wood School- Banglore. JSS Group-Banglore. Barathiya Vidyabhavan-Coachin. Army Public School- Kolkota. LTM International- Cuttak. MVR school- Nalgonda. Ratnam School- Thirupati.
Why To Sponsor ? 1 Lakhs Children participants, 8 cities 1 Lakhs+ Schools 1 Lakh + direct reach to students Media reach across - Radio, TV, Print and Web Media Marketing and Advertising reach over 60 Lakhs PR value worth over 1.5 Cr School connect opportunity & Parents connect 1 Event offering Marketing, Branding & Sales all in one with over 6 months of continuous exposure. 360 degree exposure offering Marketing, Sales and Branding opportunity all in one. Event spread over 6 months 13
Prize Distribution Back Drop Certificates Medals
School Connect Program – Our team will be visiting various schools at all the cities and shall conduct a program refereeing to NASO registration & participation Emailer & Messaging Campaigns Activation via Media (Print, Radio, Online, Outdoor, & Electronic) School Connect Program – Our team will be visiting various schools at all the cities and shall conduct a program refereeing to NASO registration & participation Emailer & Messaging Campaigns Activation via Media (Print, Radio, Online, Outdoor, & Electronic)
Expose your brand to the largest consumer segment - children Grow your business through the sale, use and showcasing of products or services. Engage your Consumers through 8 city; 6 months long Sports extravaganza. Generate right & regular sales by interacting and reaching your exact audience. Expose your brand to the largest consumer segment - children Grow your business through the sale, use and showcasing of products or services. Engage your Consumers through 8 city; 6 months long Sports extravaganza. Generate right & regular sales by interacting and reaching your exact audience.
Media Partners - Online - Offline Beverage Partner Snacks partner Prizes Partner Sponsoring any 1 sport Equipment Partner Stalls & Banners Marketing Partners Others
Title Sponsor Powered By 1Powered By 2 Co-Sponsor 1 Associate Sponsor 1Associate Sponsor 2 Co-Sponsor 3Co-Sponsor 2
ccPARTNER ENTITLEMENTSQuantity /DaysTITLE SPONSORPOWERED BY(2)Co - SPONSOR (3)ASSOCIATE SPONSOR (2)Pre/During/Post 1TITLE & EXCLUSIVITY Ration of Branding 50%25%15%10% Logo integration YES Product exclusivity YES Access to participant data YES 2PROMOTION – ON AIR * 2 Original Episode & 2 Repeat Episode (30 min) YES Push Back YES FCT YES Promos : 30 Sec x 4 times a day x 10 days YES 3PROMOTION – PRINT * - Logo in print ads YES Mention in press releases YES 3PROMOTION - DIGITAL* - Logo on event webpage with link to sponsor website YES Logo on Email Campaigns2 lacYES Logo/Name on Whatsapp Marketing10 lacYES Online Advertising - Facebook / Adwords90 daysYES Online Contest to be run on Event Page90 daysYES SMS Alerts/Announcement1 lacYES Promotion throught social media sites YES 4PROMOTION - OUTDOOR* Logo on Hoardings/Bus Stops*30 sitesYES Logo on Bus Pannels40 busesYES Logo on Static Ad in Cinema Advertising15days/10 cinemaYES Sponsor Options
21 cPROMOTION - RADIO* Mention in plug-ins + promos 8000 secYES 6PROMOTIONS – ON GROUND-VENUE Logo on Entry Arch YES Logo on Parameter Board YES Logo on Presentation Backdrop 50%35%15%10% Exclusive Banners* YES On site promotional space* YES 7PROMOTION – ON GROUND Product Sampling Opportunities YES 8PROMOTION – ON GROUND/COLLATERALS Logo on Merchandise ( Tshirt/Sipper )300YES Logo on Registration Form80000YES Logo on Posters/Teasers/Leaflets5 lacYES Logo on Miniauure Bat Personally Signed by Balwinder Singh Sandhu - as a Invitation to the School5000Yes Logo on Trophies & Medals13200YES Logo on Certificates20000YES Acknowledgement during award ceremony YES Special Invitation to senior management or delegates YES Rights to present trophies YES Total Promotional Element 36292017 Sponsorship Amount10000007500000500000250000
Contact us 22 BANGALORE Plot No 69, Kriloskar Layout. Bangalore- 586004. 8885505533. 8885505522 HYDERABAD: 1-10-28-231/31. Road No 3, Indra Nagar, Nagarjuna Nagar Colony. Hyderabad-500063. 040-65452525. QUICK CONTACT www.naso.in email@example.com 04065452525. 9966616200
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Preston's astonishing technique will change the way you approach your instrument. In his hands, a single guitar becomes numerous instruments, creating a multitude of sonic and rhythmic effects. He plays and analyzes 3 original compositions: "Slap Funk," "Border Towns," and "Tribes," teaching "rim shots," "bongo hits," slap harmonics, double hammer-ons, two-hand tapping, right hand fretting, and other techniques. By learning these tunes you'll gain myriad ideas for your own solo guitar style. Intermediate/advanced.
Since its publication in book form in 2003, THE CRAFT OF PIANO PLAYING has won kudos from pianists on every continent. The DVD version features exercises from the original book plus several new ones, complementing the book's in-depth investigations with an immediate, graphic and tangible experience of Alan Fraser's teaching. The exercises develop hand structure and function, replacing both tension and over-relaxation with effective activation based on optional skeletal alignment and offering an ever more profound technical development for the assiduous student.


text: Story time kids Click to block a category:GamingPoliticsNewsComicsAnimeOther kndz next random Comment Top NextComment next THE TRUE ENDINGS OF DISNEY? TALES Myrl as min © MIB, wll@ ! lung NM MM. she MN teii . version White", the ' , mam monther. l _ 15- 5. she had to dance in i item shown until she died. MMI Mt. hi? the them ts. g Balti A! the end. In the ' tale of The Lida lall! he !. instead (J killing nave herself. Sh! dies cuddling and ISIS!! her. Her step- eatern: In stern: f nature MAM. kt) Ability in her BM he .1 Prince. Cy MIM 1311 ' ... +1 Views: 947 Submitted: 11/04/2013 Facebook Twitter Google+ Tumblr Pinterest


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Apparently Ne-Yo wrote this song in 8 minutes so no wonder that dude has such a huge catalogue of unreleased songs leaking everyday.
Apparently Ne-Yo wrote this song in 8 minutes so no wonder that dude has such a huge catalogue of unreleased songs leaking everyday.
Done just after Bashy's album release party.
This was at the same time of that freestyle I posted earlier. I'm kinda busy so just skipped through this, but they talk about Avirex a lot.
Previously: Video: Lethal Bizzle & Ozzie B Freestyle On Westwood
He's officially the hottest artist for 2010. Drake was supposed to pass through my show last Friday, but he canceled at the last minute due to a studio session that was running late……..with Rihanna. I hooked up with him the following day, instead of doing the usual studio interview, we hit Hyde Park, which is London's equivalent of Central Park.
Drake breaks down:
*The Rihanna situation.
*Working with Kanye West
*Working with Chase & Status
*The forth-coming album and releasing it independently.
…..and more.
Full interview drops this friday.
Can you believe I missed Ashley's first and only goal of the season, plus Anelka's wonder strike?
As you all know Chelsea have an important match tomorrow in what should be a relatively comfortable win. No shots. This will be our only trophy of the season but I have a feeling that the 2009/10 season is going to be really special for us, especially if we're signing the player who I was told about earlier today. I don't even think Sky Sports News have reported it yet…
FA Cup Final: Chelsea v Everton [Saturday, May 30th @ Wembley. KO 15:00]
In part 2, The Chef talks about respect in the game, pays homage to the other members of Wu and about working with Dr. Dre & RZA.
Previously: Raekwon Interview With HHC (Pt. 1)
The Chef talks about Hollywood and how he's releasing a documentary with the upcoming album. He also lists what albums should be studied to learn about hip-hop.
Tinchy discusses the UK scene and explains the meaning behind is single Number 1. He also reveals that his album, Catch 22, is in-stores sometime in July.
FX sits down with DaVinChe to talk about his influences, how he first teamed up with Kano (no homo) and go into further details about business conduct. Dude needs to make another Brown Eyes because that's one of the G.O.A.T. UK hip-hop songs in terms of production and just in general.
Sidenote: The interview doesn't start until the 1:40 just in case you thought it was me posting up a Yard man's song by accident.
scouted @ DaVinChe's spot
Sidenote 2: This was something I was meant to do earlier but a big shout out to DaVinChe for the blogroll link, to BBC 1Xtra and Carla for seeing what I'm trying to do here so early on and placing my site under 1Xtra Recommends (in just 12 days of starting up) and Eskay, Dre and especially Nation over @ Nah Right for the inspiration and the blogroll add. Almost forgot my US blog bredren Miss Info too lol.
All this in just under four weeks and do you know why? It's because, My Blog Game Proper™


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Can't afford Audio Research's flagship Reference products? Well, welcome to the club!
But AR is also now offering much of that same technology in what they're calling their "Foundations" line of equipment.
The VT80 power amplifier, for example, puts out a conservatively rated 75 watts per channel into either four or eight-ohm speakers and sounds snazzy doing it. It looks like a million bucks and has some twenty-first century technical tricks up its sleeve.
Watch the Secrets website for the upcoming full review!


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9ct Yellow Gold Wide Texture Creole Hoop Earrings£225
Product code: 4811364
Return within 30 days for full refund or exchange.Delivery Info Returns Info
Stunning 9ct gold creole earrings with flowing edges and an extravagant clasp that give the design an extra flourish. If you're looking to make a bold statement then these gold creole earrings are the perfect match
Elegant jewellery for all occasions
- Earring style
- Creole
- Material
- 9ct Yellow Gold
- Height
- 2.2 cm
- Width
- 0.6 cm
Delivery & Returns Information
Standard delivery costs £3 for orders under £100 and is free when you spend £100. You can have your order delivered Next Day delivery to any address in the UK for £5 or purchase online and have your order delivered to store for free. View all delivery information.Return within 30 days for full refund or exchange.


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2301 S JOYCE ST, Arlington, Virginia
$799,0003 Beds2+ BathsActive
$799,0003 Beds2+ BathsActive
2301 S JOYCE ST, Arlington, Virginia 22202 - Beautiful Brick Aurora Highlands Colonial on tree lined S. Joyce Street. This home is complete with wood floors, fireplace, updated kitchen with granite and SS appliances, separate dining room, built-ins, fully finished basement, brick paved patio, fenced in backyard. No need to worry about parking with off street parking in your private driveway.
View Virtual Tour
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"The ups and downs of a real estate search and transaction can be daunting and draining. Having a trusted team of professionals in your corner for counsel, reinforcement, perspective, and friendship is essential in both reaching your real estate goals and also keeping your sanity during the process. They are knowledgeable about everything from financing to marketing to the local market trends and, most importantly, are a pleasure to deal with every day. They helped us navigate a few very perilous last minute developments during our purchase and never lost their cool and reasoned approach to moving forward. All in all, DC Home Buzz is a tremendous advocate for their clients. We recommend with the highest confidence."


text: Skt., lit., "Diamond Being"; in Vajrayana Buddhism, the principle of purity and purification. Vajrasattva embodies the capacity to eliminate spiritual impurities of all kinds, particularly neglected commitments toward one's teacher and ones' own spiritual development. Vajrasattva is a sambhogakāya manifestation; he unifies all the five buddha-families within himself in the same way that the white color of his body (in iconography) unifies all the five colors. With his right hand he holds a dorje to his heart, which signifies his indestructible essence. His left hand, holding a bell, rests on his hip; this is an expression of his compassion. The hundred-syllable mantra associated with him is used in all schools of Tibetan Buddhism for purification of the mind.


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Doctor insights on:
Underwear Cause Cancer
Cancer (Definition)
Cancer is a group of diseases that is characterized by uncontrolled cell growth leading to invasion of surrounding tissues that spread to other parts of the body. Cancer can begin anywhere in the body and is usually related to one or more genetic mutations that allow normal cells to become malignant by interfering with internal cellular control mechanisms, such as programmed cell death or by preventing ...Read more
Does wearing tight underwear cause testicular cancer?
No: Wearing tight underwear is not a risk factor for testicular cancer. A pssible risk factor for testicualr cancer is an undescended testicle present at birth but clothing is not a risk factor for the future development of testicle cancer. ...Read moreSee 1 more doctor answer
Can soiled or sweated underwear be cause of testicular cancer?
No: Testicular tumors can be divided into three groups, prepubertal, adolescent,and adult or seminoma. They originate from germ cells at different stages of development. The most common ones arise in postpubertal men and are characterized genetically by having one or more copies of an isochromosome of the short arm of chromosome 12 [i(12p)]. Unrelated to underware. ...Read moreSee 1 more doctor answer
I have urinary incontinence and my underwear is always wet, I use baby powder as a panty liner. Does baby powder cause uterine or ovarian cancer?
Please see a urologi: Urologist as you need a good evaluation to help you control the incontinence as you may be a candidate for meds or surgery. ...Read more
Could it be a good idea to wear long underwear in the summer for protection from skin cancer?
Too hot!: Depending on where you live, it might be too hot to wear long underwear during the summer! you should protect your skin with sunscreen (spf 30), hats, uv protective shirts, and minimize your sun exposure. Enjoy the summer! ...Read moreSee 1 more doctor answer
Can you get testicular cancer by wearing tight underwear?
No: Tight underwear is not known to be a risk factor for testicular cancer. ...Read more
Itchy pink growth on labia majora. Seems to itch more when it rubs against underwear or when I sweat. Sometimes swells then goes down. Is it cancer?
Not likely: There are many possibilities from ingrown hair, yeast, bacteria.....other skin conditions. I would have it looked at though. Cancer would be very low on the possibilities ...Read moreSee 1 more doctor answer
Can earrings cause cancer?
You decide: Don't get your ears pierced until you're very sure it's what you want and know you're not a keloid former. The fact that many people get keloids from this gave rise to the myth that it "causes cancer" -- keloids aren't cancer but are troublesome. ...Read more
Does nailpolish cause cancer?
No: When used on nails or skin it has no known risk. If ingested on a regular basis it could feasibly cause many sorts of problems, but this is not studied obviously. ...Read more
What is the main cause of cancer?
It is genetic accide: It is not possible to answer your question exactly they way you may like to see as we cannot pinpoint in any one individual, what caused his/ her Cancer. But on a population basis the causes are multiple, including smoking, excess use of alcohol, Aging, immune deficiency, virus infections and Chemical exposure to DNA damaging agents like Radiation from multiple sources. Overeating and Obesity also ...Read more
- Talk to a doctor live online for free
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- Soiling underwear causes
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- Ask a doctor a question free online
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O'FALLON — As they watched the last games play out Saturday afternoon at the IHSA Boys State Bowling Tournament at St. Clair Bowl, the Jersey High boys bowling team saw a red-hot Cahokia team jump over them and Tinley Park Andrew into seventh place.
That act, which pushed the Panthers down to ninth place in the final standings, was just about the only disappointment over a two-day tourney that saw Jersey stake out a spot among the best teams in the state.
Jersey, making its first trip to the state tourney, finished ninth with 12,747 pins. Thus, the Panthers' dream season ended on the last possible day – the second day of the state tourney.
"It's been an amazing season with a great group of kids," Jersey coach Chris Skinner said. "There is no way I can be disappointed at all. I mean, we were co-conference champs with Highland, won a regional and got all the way to the second day at state.
"There are a lot of teams that would like to trade places with us, I'm sure."
Among those would likely be Alton, which was 13th after Friday's action and fell just 28 pins short of the final second-day qualifying berth. The top 12 teams from the first day at state advance to the last day.
The Redbirds did advance to individuals to Saturday's finals. Junior Tyler Stevenson finished 48th overall with a two-day total of 2,595 pins. He had series scores of 601 and 685 Saturday and a high game of 256. Teammate Chris Perkey, a senior, finished 57th overall with a grad total of 2,558. His series scores Saturday were 568 and 674 and his high game score Saturday was a 232.
"We knew it would be a bit of a long shot for either of them to finish in the top 12," Alton coach Jeff Woszczynski said, "but they still each had a good day.
"I told them to go out and have fun with it today."
Lincolnshire Stevenson won the team championship with 13,381 pins followed closely by Rockton Hononegah with 13,321. State tourney host O'Fallon was third with 13,240, followed by Machesney Park Harlem and Lockport tied at 12,1110 each, Orland Park Sandburg 12,915, Cahokia 12,825, Tinley Park Andrew 12,816, Jersey 12,747, Plainfield North 12,635, Collinsville 12,579 and Chicago Morgan Park 12th at 12,476.
"We were already finished and we were watching the late scores come up," Skinner said. "We were waiting on Cahokia to come in. They bowled an 1,149 last game and got past us."
Stevenson's Zach Singer was the individual champion with a grad total of 2,899 over the two days and 12 games of competition. Andrew Orf of O'Fallon was the top area finisher, coming in at third place with 2,863.
Jersey junior Jeff Gump was the Panthers' top finisher, coming in at 21st place with 2,689 pins. His series scores Saturday were 678 and 645 and his top game score was a 248.
Jersey senior Jacob Freand finished 25th with a grad total of 2,664. He had series scores of 651 and 670 Saturday and his top games were a pair of 243 scores.
Senior Brandon Handler finished 45th with a grand total of 2,605. Saturday, he had series of 659 and 674. His top game was a 255.
"Those three guys who had really nice tourneys," Skinner said. "Overall, our team did a good job. Our guys are always there for each other and there's always been someone to step up when we needed it."
The rest of Jersey's scores included senior Dane Farmer who had a grand total of 2,395 on Saturday series scores of 569 and 555 that included a top game score of 201, and senior Kevin Mangrum, who had a two-day total of 2,394 on Saturday series scores of 591 and 608 with a high Saturday game of 224 in the first game of the day.
"These boys finished as a top 10 team at state," Skinner repeated. "That sounds really good."
Reach Pete Hayes ay 463-2500 or Twitter @HayeserPete


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Most Earned
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June's been a big month in terms of backwards compatibility, with eight more titles - from a variety of publishers - added to the program today, bringing the total up to twenty for this month alone.
Posted 10 months ago by Andy Mills, 104 comments
After what most people considered a disappointing couple of weeks, this week's Deal of the Week will hopefully go some way to putting a smile back on your face. Microsoft has given 50% discounts to t
Posted 6 years ago by Dave Horobin, 106 comments
THQ, the publisher behind Red Faction: Guerrilla, Red Faction: Armageddon and the downloadable tie-in, Red Faction: Battlegrounds, have decided to cut their losses with the Mars-based franchise. THQ
Posted 6 years ago by Dog of Thunder, 71 comments
A new multiplayer mode and new vehicles have been added to Red Faction: Battlegrounds. The "Origins Pack" DLC adds two new achievements worth a total of 25G. Blast, burn and blow away the competition
Posted 6 years ago by Rebecca Smith, 11 comments
When you're tasked with helping to save a planet, its vitally important that you have a strong and capable team to assist you. Volition's forthcoming third-person action adventure Red Faction: Armage
Posted 6 years ago by Keith Gray, 29 comments
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text: Hello great and good people. Barnowl stepson just received his decree nisi. Divorce of wife of 8 years. Assets: Pre marital/post marital flat, has lived in rented accomm for 3 years now with family because of better schooling area for 2 daughters 4 & 7. Flat open market value 160k Has own business, wife never shown interest, dividends to shareholders 60k per annum assets prob around 25k. Question, If she decides to get shitty at this late stage what is she entitled to and what if anything he can sell to interested partners for £50 to stop the leech? I'm sure its been done to death before but I just want to know in case I get asked to take ownership of something.


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Gordon is one of the RSPB's satellite tagged turtle doves and he has been telling the readers of the children's nature magazine Eco Kids Planet about the long journey he is preparing for, back to the UK from his winter home in Africa, and the plight faced by him and his fellow turtle doves… As Gordon […]Continue reading
Turtle Dove Talk
All For The Love of a Dove
Thank you to Robert Yaxley for this update on the Dove Step 3 Challenge from Spain… As you read this, Jonny Rankin and myself with the help of support drivers Sven Wair and Malcolm Fairley will be halfway through a monumental physical walking challenge. The march from Tarifa to Gijon, crossing mainland Spain from south […]Continue reading
Just 48 hours until the Dove Step team embark on an epic walk to save the Turtle Dove
It is our pleasure to invite Jonny Rankin to give this guest blog today as he is busy making last minute preparations for his next incredible fundraising journey. I type this just 2 days away from our next Dove Step journey; our most daunting yet. We have deliberately made each fundraising effort something suitably […]Continue reading
An update on the tagged Turtle Doves
Guest blog by Dr John Mallord, Senior Conservation Scientist, RSPB Centre for Conservation Science Titan our 2014-16 satellite tagged Turtle dove was a superstar; the first UK-breeding turtle dove that was followed on its entire migratory journey from his breeding grounds in Suffolk to Mali and back again – and back to Mali a second […]Continue reading
Give a home for turtle doves this Christmas
Our 'two turtle doves' of Christmas may well be sunning themselves in sub Saharan Africa right now, but that shouldn't stop you from giving them a thought and thinking about what you might be able to do over the next coming months that will provide them with a welcome return in spring. Operation Turtle Dove (OTD) […]Continue reading
Operation Turtle Dove reaches millions on The One Show!
Did you see last night's episode of The One Show on BBC 1? At the end of the programme we were treated to stunning footage of turtle doves at Glebe Farm in Norfolk. The farm is part of Operation Turtle Dove and special habitats have been created to provide valuable nesting and foraging areas for […]Continue reading
State of Nature
Jamie Wyver, from Operation Turtle Dove, comments on this week's publication of the second State of Nature report… As our turtle doves head off to Africa for the winter this week, conservation experts have been flocking to the launch of the second State of Nature report. Fifty three conservation organisations joined forces to produce the […]Continue reading
Meet Myrtle the turtle dove
One of Titan's successors, Bird 161004, has a new name! He will from now on be known as Myrtle. Myrtle was the most popular name in the naming competition run by RSPB Science. Thank you to Claire Wilson, Anne Tomma, Howard Bayley, Jez Elkin, Hannah Gumbrell, Katy Spedding, Halina Morton, Susanna Allen, Ella Wooley, Julie […]Continue reading
Help us name our satellite tagged Turtle Dove!
Bird 161004 is waiting to follow in the slip stream of Titan, the first turtle dove to be tracked from his breeding grounds in the UK to his wintering grounds in Africa and back, and needs a name! 161004 is one of 6 newly satellite tagged turtle doves, which were fitted with light weight backpack […]Continue reading
East Anglian turtle doves will reveal more migration secrets
Six new satellite tagged birds to build on legacy of Titan – the first UK turtle dove tracked to Africa and back Six turtle doves are being satellite tracked from their breeding grounds in the UK to their wintering grounds in West Africa, to help scientists better understand why numbers are crashing so rapidly. According […]Continue reading


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The Bank of England stoked fears that interest rates would rise sooner rather than later after it yesterday raised its inflation forecast partly to reflect the impact of the Budget.
The Bank's Monetary Policy Committee said in its quarterly Inflation Report that the risk of underlying inflation overshooting its 2.5 per cent target in two years was now 68 per cent, against its February prediction of 57 per cent. Inflation was predicted to hit 2.6 per cent in the second quarter of 2004 and while inflation was likely to sit below target until then, the risks were that the projections might rise.
Higher public spending twinned with an upturn in global demand would offset weaker consumer spending, to push annual GDP growth to 3 per cent by the end of the year, against a prediction of 2.4 per cent made in February. It will exceed 3 per cent by the end of 2003.
Gilts fell sharply amid perceptions that the report's revisions showed the MPC to be more hawkish – inclined to raise interest rates – than supposed. The committee's remit is to set interest rates at a level that will keep inflation to within 1 percentage point of 2.5 per cent.
While Mervyn King, the Deputy Governor, said interest rates would at some point have to return to a "normal" level, he warned against over-interpreting the report.
"There is enormous uncertainty here," he said. "I would caution against drawing too much from small changes in assumptions. [Setting interest rates] is a decision that you can take one month at a time."
John Butler, UK economist at HSBC, said: "Given this new set of forecasts it is odd that the MPC did not raise rates at the last meeting. This undoes all the recent MPC comments. It raises the risks rates could go up as early as June."
The Bank said the economy had troughed in the last quarter of 2001, yet it warned that there were still risks that the growth target might slip. Hard evidence of economic recovery had yet to emerge and amid the prevailing uncertainty interest rates had last week been left unchanged at the 38-year-low of 4.0 per cent.
Mr King also said that while the ratio of average earnings to property prices was not at its peak, it was prudent to "take action" before the peak was reached. "There is no doubt that these rates of [house price growth] are unsustainable. But there are many other things in the economy that are unsustainable," he said.
Earlier, official data showed unemployment rising to 3.2 per cent, with the claimant count rising by 5,400 to 953,000 in April despite predictions of a fall. Average earnings growth was also up, rising 2.9 per cent in the first quarter. Meanwhile, US industrial production registered a 0.4 per cent rise for April and business inventories fell for the 14 consecutive month.
The Bank's optimistic mood was echoed at the annual meeting in Paris of ministers of the 30 nations belonging to the Organisation for Economic Co-operation and Development, the so-called rich nation's club. The Belgian Prime Minister, Guy Verhofstadt, said: "The general feeling around the table was of a cautious optimism that in the next months and of the second part of the year we should see and feel the economic recovery."
Pedro Solbes, the European Commissioner for monetary affairs, said: "We think generally that the worst is behind us."
Paul Boateng, the financial secretary to the Treasury, refused to comment on whether he thought the Budget would lead to hikes in interest rates.Reuse content


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- Fours
- Sixes
- Wickets
- AN Cook 10*
- IR Bell 26*
- M Morkel 0/19
- LL Tsotsobe 0/18
Morne Morkel will open the bowling...two slips in place
Here come the umpires! South Africa get into a huddle. England's fight to retain the No. 1 spot is able to begin. No immediate change to the order as Alastair Cook and Ian Bell walk out
2.55pm The cover is now completely off. We may just get a start here. And then they'll try and stay on during light rain. Pitch, unsurprisingly, is quite green. But the ball will also get damp.
2.50pm Thanks Alan. And I come with bad news. Spots of rain in the air and the main cover is back on. Well, it changes again. The cover has been moved aside. It's teasing us. The stumps are in the ground.
2.45pm On Sky, they're showing the toss. Don't worry, South Africa still won it and chose to bowl ... Anyway, with the prospect of the some actual play, I'm going to hand the Rain Watch baton back to Gnasher. Cheers
2.40pm Right, back to business, then. AB de Villiers and co are warming up on the outfield, which is a hive of activity. Only the wicket remains covered and while the skies are grey it isn't raining. Get out there and play, lads, while you still can!
2.35pm This, from Jeff in the US, is so bad it's genius: "I guess we'll just have to let Bydgoszcz be Bydgoszcz then."
2.27pm Actual Cricket News from Wales: That latest roll of the dice is a 3pm start, with 24 overs per side. Just don't glance at the rain radar.
2.25pm You wouldn't have predicted it at the start of the day but the word on everyone's lips right now is "Bydgoszcz". Not that any of us can pronounce it ...
"Wojciech's comment of 'light but quite intense rain' has got me perplexed," says Mahinder Vagel. "Was the rain lighly intense or intensly light?!"
And Gav is struggling with the news: "So you're telling me that my hopes for a Polish Premier League are now ruined??" Don't worry, Gav, you can be sure that when they get one up and running, Chris Gayle will be snapped up by the Krakow Fire Dragons within days.
2.15pm "Not quite true about baseball," observes Alan Tipler. "Occasionally a rained-off match can become a double header the next day but if the rained-off game is the last of a series the lost game is played later in the season when both teams are available. They play 6 days out of every 7 and have to travel vast distances between each 3 or 4 game series." Yes, but most of the time, they're just sitting in a dugout, aren't they? What's the longest-ever at bat? Ten minutes? And then there's half-a-dozen pitchers to rotate ... No wonder they have to play 160 games a season to earn their dollars.
2.10pm It's still drizzling down at Cardiff. The crowd looks to have thinned out a bit now - and if the Welsh have given up on a dry afternoon, you know you're in trouble.
2.05pm I've been doing a bit of research and it seems Bydgoszcz doesn't currently have a cricket club. Wojciech, I think the whole of cricinfo's readership is behind me in saying: you need to sort this out.
2pm "I suspect Messrs Yardy, Gale, Trescothick and Adams might have something to say about moving this match to tomorrow..." Chris rightly points out that Cardiff has a kiss-me-quick date with FLt20 finals day arranged for Saturday.
Eamonn, meanwhile, doesn't think losing this one to the weather will be a disaster: "@Antonio: Oh no! Not another washed out ODI, I mean it's not like these things are played almost non-stop all over the world..."
And Gav thinks the ICC may not be the administrative cynosure we all thought: "What authority does the likes of the Cardiff City Council have over the unified and glorious might of the International Cricket Council? Oh hang on..."
1.55pm News from Poland, cricket's new frontier. "In my own city, Bydgoszcz, we had light but quite intense rain from 11am until 2 pm and... it's all over, now is blue sky, sun and fresh air... what is wrong with you England? Rain, stop (15 minutes) rain, stop (15 minutes), rain.. again and again and again..." You've summed it up pretty well there, Wojciech. How are Bydgoszcz CC doing this season, by the way?
1.45pm "The baseballers here in the US play 162 games in the regular season/year, and games simply get pushed to the next day in the event if inclement weather. How many years will it be before cricket can finally figure out how to simply use an extra day, or what about the wild notion of turning it into a day/night game? Is it really that difficult?" I think, Antonio, the amount of rain/reserve days would stack up to such an extent it would become a towering disaster. The calendar is crowded enough as it is. Extending a regular game into a day/nighter is a more sensible option but would probably fall foul of council restrictions on the unplanned use of floodlights, or some similar bureaucratic nonsense. Yeah, I know.
1.35pm Perhaps the only solution is to pretend they're not even interested in playing, don't announce a start time, and just run out as soon as the clouds pass through for a five-over thrash. D'you think that would trick the rain gods? The cut-off time, just to remind you, is 3.32pm.
"England should play their home matches in UAE and not Pakistan," suggests Salman Bhatt. Knowing England cricket, games there would probably be abandoned due to an overly dazzling sun ...
1.30pm The
sun has come out over the Costa del Cardiff and the locals are now prancing around in ill-fitting beachwear heavens have opened again. Heaviest rainfall of the day so far, apparently.
1.25pm Looking at that rain map, there appeared to be a clear band sweeping over ... and so it is: we're now looking at a 1.45pm start. Will bring you news of any further reduction in overs as and when we get it.
1.20pm "What happens to the rankings if the match is a draw?" wonders pb. "Will the winner of the 2nd ODI then become No. 1?" Yes, if this becomes a no-result it should just roll over. And when that game is rained off, it will fall to the third, and so on ...
Here's James: "Breath now fully released. Just looked at BBC weather for Cardiff. Rain disappears about 8pm. Not good." As you can see from the rain radar, it's pretty filthy looking down in the south west.
1pm I told you not to hold your breath - look, now, you've gone all blue in the face. The overhead sprinkler system is back on at the Swalec and that 1.15pm start time has been scrapped.
12.50pm Hello... The rain has stopped again and the covers have been peeled off the square. Or are they being rolled out again? It's all a touch Hokey Cokey out in the middle. Anyway, a new start time of 1.15pm has been mooted. Don't hold your breath.
12.45pm I'd love to tell you we were about to start playing a 38-over match. Love to say that. But the rain has returned and those brave Welsh souls in the stands are glistening like wet otters, as Boris Johnson would likely have it. There is, however, some cricket successfully taking place in the shires - Division One leaders Warwickshire have lost a couple of early wickets in pursuit of 367 to beat Middlesex, while surprise challengers Sussex are four down halfway to their target of 160-odd. You can follow the action on our county blog.
12.35pm Arrrghh. The rain has returned so that start time looks unlikely. On that frustrating note I'll hand over to Alan Gardner.
12.30pm Just a hint of rain in the air again. The covers are poised but the players are still warming up. The Powerplays are eight overs, four bowling and three batting if we get 38 overs per side. Wonder if either side would have selected a different team if the rain had come before the toss?
12.15pm If there is no more rain we will get a 38-over per side match.
12.05pm Good news, folks. Play will start at 12.45pm as the rain clears. It might not stay away, though. Will bring you details of overs etc when we get them.
12.00pm For those of you who like to keep track of such things, as the toss has taken place this match will now count in the records even if there is no play.
"England beat WI for 5th place in the under-19 WC," says John Muir. "Reece Topley looks a great prospect- 19 wickets in 6 games at an average of 9.10 and economy rate of 3.17 and he's only 18. He's a left-armer, too. One to watch." Indeed he is, very exciting. Essex have another, too, in Tymal Mills.
11.50am Maybe some more positive news from the ground...the rain appears to be stopping and the umpires are having a look.
Oh, well, that was brief. Drizzle is back. The umpires have left. As you were.
In the UK we are getting highlights of the World Cup match between these two teams from Chennai. It was a thriller. Stuart Broad has just tweeted: "I know the result but I'm nervous watching it!!"
11.45am Paul Flounders has asked about the most consecutive ODI wins by a team. England are currently on ten, but have a long way to go. Here's the record
"As many as 7 sothpaw's in proteas squad." says Sharad. "Hmm i can see Swanny smiling."
PDTM asks: "If there was only enough time for a 20 over game, would it be a T20, or just a short ODI? Could have some ranking implications..." Just a short ODI, not classed as a T20.
11.35am It's funny who you meet doing BBB. A mate from university, Richard, has got in touch. "Alright Andy how you doin? What do you guys think about Tim Bresnan looking like he is not going to be allowed to bowl 4 or maybe 8 overs at the t20 finals day tomorrow? Or if it's washed out today will he be released?"
Hey Rich...I suspect he'll be released for Yorkshire if there's no play and there's a decent chance of it even if there is a shorter game. Good luck to your boys tomorrow.
Meanwhile, PDTM as this: "@Andy: It wouldn't be inconceivable to play Bairstow, Kieswetter and Prior in the one day team (if there were enough spots going). None of them have done much wrong with the bat."
11.30am Full covers still in place. Pretty dank scene at the moment. The match has to start by 3.32pm for us to get a 20-over contest.
Boogoo isn't happy: "August Bank Holiday and they put ODI's on the Friday and Tuesday? No disrespects to cricinfo but what about the poor office worker who wants to actually watch the cricket?"
"Why would you need both Kieswetter and Bairstow in your side?" says Andy. "Bairstow's keeping is more than competent, so he could bat at six and allow you to play five bowlers/allrounders."
11.15am Here's Chris: "England are in a very rare situation of having too many bastman to fit into the team. I'd like Cook, Bell, Trott, Bopara, Morgan, Bairstow, Keiswetter but if you have Patel as well you're left with 20 overs from Patel/Bopara/Trott which puts a lot of pressure on the 3 front line bowlers."
11.00am It's still raining. "100% agree with Sumith," says Thomas. "Peter Trego has been in the last 3 T20 final days but yet ECB Selectors dont bat an eyelid."
Quite a few questions about Albie Morkel. He suffered a recurrence of his ankle injury against Gloucestershire so suspect South Africa didn't want to risk him.
10.45am Although we are in 50-over mode today (or should be, at least) thoughts are turning towards the World Twenty20. Here is ESPNcricinfo's latest selection of players to keep an eye on.
Here's sumith: "Query : Are Peter Trego and Ryan ten Doeschate eligible to represent England internationally ? If yes then they should be included asap. Two brilliant players underused in world cricket." Trego yes, ten Doeschate no.
10.35am While wait for a break in the weather here, there's a Test match going on in Hyderabad and you can follow it here.
"Correction Gnasher," starts Graeme from South Africa. "SA are now using Robbie P as their main SLOW bowler. Spin is only incidental, and, one must sometimes assume, accidental, to his bowling." Haha. Similar things were said about Paul Harris.
Another Saffa (well done with that) says: "I can't believe Matt Prior is not in the one-day side! His keeping has improved massively in the last couple of years, and with Pietersen's future uncertain, I think he is easily the most dangerous batsman in the test side."
"Why do England persist with Eoin Morgan?" asks Neil Dyer. "Surely Jonny Bairstow deserves the nod ahead of him?" Did you not see his innings against Australia at Lord's? A one-day matchwinner.
10.30am "I must say i'm particularly disppointed that Bairstow didnt get the nod ahaed of Bopara," says Peter. "I dont understand why the England set-up choose to go with someone that has been tried and tested and pretty much been failing every such test lately. Jonny deserves a go. As a South African i would much rather see Bopara in the side! Lets hope he doesnt prove me wrong ..." To be fair to Ravi he had a very good series against Australia earlier in the summer.
"Come on Peter!" says Nix. "You should have watched Bopara bat against Australia. It was due to him and Bell that the Aussies weren't even able to dismiss the top 4. I have not been a fan of Bopara but his recent performances have been excellent."
10.20am A few of you asking where Johan Botha is for South Africa. It's an interesting story. We'll see him in the Twenty20s, but after the World Twenty20 he will be released from CSA contract to captain South Australia. So, in ODIs, they are now using Robin Peterson as their main spinner.
10.15am We should be starting now but we aren't. The full covers are going on. Start delayed by rain. Could be a long day, folks.
"My opinion is that this Proteas bowling attach has been led by Morkel for a few years now, ably supported by Tsotsobe of late," says Saffa from, yep, South Africa. "Steyn's absence will not hurt us, but it would have been nice if Albie was in, instead of Parnell."
10.10am Sadly the covers have been brought on moments we were due to start. It's not heavy rain yet but the radar images suggest that is on the way. Meanwhile, plenty of debate about Ravi Bopara keeping his place ahead of Jonny Bairstow despite his recent personal problems leading to a lack of cricket and Bairstow's excellent Test at Lord's.
10.00am Dean Elgar is making is ODI debut today and has big shoes to fill as he replaces the rested Jacques Kallis. I remember watching him at an Under-19 World Cup and he looked a good player. Bowls some left-arm spin as well.
"South Africa seem to lack a bit of power hitting," suggests Dan. "And with Duminy, du Plessis and Elgar all capable of bowling spin, dont quite see why we have picked so many bowlers."
9.55am I do have to say that the forecast for today is not good with a band of heavy rain moving across the country. It's dry and the moment, but we'll do well to get a full day's play. Fingers crossed, though. Plenty to play for.
9.45am Hello everyone and welcome to the start of the one-day series between England and South Africa. It's another battle for No. 1. Alastair Cook has been presented with the ICC one-day shield, but his team will lose it if they slip up today in Cardiff. I'm Andrew McGlashan and along with Alan Gardner will bring you all the action.
The first news of the day is that South Africa have won the toss and will bowl. No Dale Steyn for them today which is a shame. England, meanwhile, have replaced Stuart Broad with Chris Woakes.
England 1 Alastair Cook (capt), 2 Ian Bell, 3 Jonathan Trott, 4 Ravi Bopara, 5 Eoin Morgan, 6 Craig Kieswetter (wk), 7 Tim Bresnan, 8 Chris Woakes, 9 Graeme Swann, 10 James Anderson, 11 Steven Finn
South Africa 1 Graeme Smith, 2 Hashim Amla, 3 Dean Elgar, 4 AB de Villiers (capt & wk), 5 JP Duminy, 6 Faf du Plessis, 7 Ryan McLaren, 8 Wayne Parnell, 9 Robin Peterson, 10 Morne Morkel, 11Lonwabo Tsotsobe
Morkel to Cook, 1 wide, down the leg side and England are off the mark with a wide
Out come the players. Haven't we just done this bit?
The game will restart at 3.10pm and it will be 23 overs per side. We'll try again.
3.05pm I know it's hard for umpires, but really think they should be playing. Ah, the cover is coming off to loud cheers.
Our man at Edgbaston, Jon Culley, has tweeted: "Off for rain at Edgbaston. 86-5. No slow handclap here."
"Who says England can't afford to open with Cook in the shorter formats?" says Dave. "At this late stage in the day he is still unbeaten and England are scoring at an infinite run rate."
Oh dear! After one ball, not a legal delivery at that, the rain is back and players are walking off. There is only another half an hour of time to play with. It doesn't really look that hard. No major rush with the covers except over the 22 yards. Now some slow hand-claps going around the ground. Very few umbrellas up at the moment.
"Why not open with Kieswetter - it's basically a 20/20 game now!" says Jake. It's a valid point.
Morkel to Cook, no run, back of a length on leg stump, tucked out to midwicket...this game now has a legal delivery
Morkel to Cook, no run, outside off stump, angled across Cook who leaves it alone
Morkel to Cook, no run, short of a length outside off, Cook happy to let another one go past off stump...no great pace in the surface
Morkel to Cook, no run, beaten this time, closer to off stump but it's this ball that Cook shapes to cut and is cramped for room
Morkel to Cook, no run, fuller on off stump, pushed firmly into the off side
Morkel to Cook, no run, full outside off, Cook leaves alone again
End of over 1 (1 run) England 1/0 (RR: 1.00)
- AN Cook 0 (6b)
- IR Bell 0 (0b)
- M Morkel 1-0-1-0
Lonwabo Tsotsobe, the No. 1 ODI bowler, shares the new ball
Tsotsobe to Bell, no run, on off stump, Bell stays back and defends towards mid-off
Tsotsobe to Bell, no run, pulls the length back shorter, Bell advances towards the bowler and opens the face to play to backward point
Tsotsobe to Bell, no run, fuller and wider outside off, Bell chases but doesn't make contact and thinks it should be a wide...just okay
Tsotsobe to Bell, 1 run, first run off the back, short of a length on off stump, Bell opens the face down to third man
Tsotsobe to Cook, no run, adjusts well to the left hander, full on off stump, Cook comes forward and pushes to the off side
Tsotsobe to Cook, no run, beaten outside off, some late away movement from Cook who pushes away from his body without any footwork
Tight start from South Africa
End of over 2 (1 run) England 2/0 (RR: 1.00)
- AN Cook 0 (8b)
- IR Bell 1 (4b)
- LL Tsotsobe 1-0-1-0
- M Morkel 1-0-1-0
Morkel to Bell, no run, back of a length on off stump, Bell defends square into the off side
Morkel to Bell, no run, tries something more aggressive, just gives himself a touch of room to drive but can only clump the delivery back to Morkel
Morkel to Bell, 1 run, angled into Bell who works it into the leg side short of mid-on and scampers the run
Morkel to Cook, 1 run, run out chance! Full delivery pushed towards mid-off and Cook would have been miles short if Parnell had hit but he missed the stumps by a huge distance
Morkel to Bell, no run, play and a miss...back of a length, Bell tries to go through the off side and moves slightly to leg and the ball ends up missing leg stump
Morkel to Bell, no run, an ugly heave into the leg side but he can only pick out mid-on
End of over 3 (2 runs) England 4/0 (RR: 1.33)
- IR Bell 2 (9b)
- AN Cook 1 (9b)
- M Morkel 2-0-3-0
- LL Tsotsobe 1-0-1-0
Tsotsobe to Cook, FOUR, good shot, full outside off, Cook leans into a powerful cover drive and even across the slow outfield the ball beats the fielder
Tsotsobe to Cook, no run, short of a length outside off, Cook shapes to cut and is beaten on the top edge
Tsotsobe to Cook, 3 runs, takes a stride down the pitch and lofts this over cover, not timed as well as earlier in the over and it pulls up short of the rope
Tsotsobe to Bell, FOUR, cracking shot, down the pitch to a fullish ball on off stump and handsomely driven up and over mid-off...one bounce over the boundary
Tsotsobe to Bell, 1 run, coming at the bowler again, doesn't connect cleanly with this drive and it runs along the ground to mid-on who is on the edge of the circle
Tsotsobe to Cook, no run, outside off, driven without much timing into the covers
End of over 4 (12 runs) England 16/0 (RR: 4.00)
- AN Cook 8 (13b 1x4)
- IR Bell 7 (11b 1x4)
- LL Tsotsobe 2-0-13-0
- M Morkel 2-0-3-0
Morkel to Bell, SIX, huge blow! Clean strike from Bell. Uses his feet to Morkel - which is no mean feat - then picks up over the leg side and the ball comfortably clears the boundary
Morkel to Bell, 3 runs, now he goes inside out through the off side, gave himself a touch of room and drove this from around middle and leg stump through the covers
Morkel to Cook, 1 run, full on off stump, opens the face and sends it fine of backward point down to third man
Morkel to Bell, SIX, shot! Bell's flying. Opens his stance to free up the off side then drives him with a straight bat high over mid-off...he isn't in the Twenty20 squad
Morkel to Bell, no run, tries to glide this through the off side and is beat by ball that is a touch shorter
Morkel to Bell, no run, full on off stump, opens the face to try and place into a gap at cover but picks out a fielder
End of over 5 (16 runs) England 32/0 (RR: 6.40)
- IR Bell 22 (16b 1x4 2x6)
- AN Cook 9 (14b 1x4)
- M Morkel 3-0-19-0
- LL Tsotsobe 2-0-13-0
Tsotsobe to Cook, 1 run, dragged down short outside off stump and pulled to deep square-leg
One wide slip in place
Tsotsobe to Bell, FOUR, lovely placement from Bell, he walks across the crease then flicks a back-of-a-length delivery over short fine-leg
"Oh boy! I am ready to pay double to see those 2 sixes of Bell again!" says Altaf
Tsotsobe to Bell, no run, well-timed again, on off stump and driven but straight to the man at cover
3.45pm The match has been abandoned. The heavy burst of rain that has come across the ground has put paid to everyone's best efforts. It has always looked it would end this way today.
So after this false the start the series moves to Southampton on Tuesday. At this stage the forecast is a little more promising. Before then, however, is Friends Life t20 Finals Day tomorrow. Yorkshire, Sussex, Hampshire and Somerset are all chasing the title. We'll have ball-by-ball commentary for you from both semis and the final starting from 11am on Saturday. If you can, please join us. Until then, From Andrew McGlashan and Alan Gardner, it's goodbye.
3.35pm Not exactly sure how much time is left to work with, but it's probably about 25 minutes or so.
"What are the England selectors on!" starts MayurRao "Not having Prior and Bell in the T20 squad? They are proper stroke makers, clean hitters! No sloggers!" Point is, without those two the Twenty20 team has done pretty well.
Gah! The rain is back. Off the players troop. What a miserable day, and just when Ian Bell was starting to motor.
"Morkel without the self-confident Steyn goes into a Harmison/Mitch Johnson pie-throwing routine," suggest David. "The difference between the two sides is on the bench."


text:
An international coalition of 29 countries has met in Paris to discuss a coordinated response to counter the threat of Islamic militants in Iraq and Syria.
Earlier the French announced that they would join Britain in operating reconnaissance flights over Islamic State positions to assist the US airforce currently engaged in strikes against ISIL.
Speaking at the opening of the conference French President Francois Hollande said the world is facing a global threat which needs a global response.
However, one country that won't be joining the coalition is Iran. Ayatollah Ali Khameni tweeted that Iran has rejected any cooperation with the US to combat ISIL.
A number of Arab countries have agreed to support attacks against Islamic State militants.
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|Publication number
|Publication type
|Application number
|Publication date
|Jan 2, 1990
|Filing date
|Jun 7, 1985
|Priority date
|Dec 12, 1980
|Fee status
|Also published as
|Publication number
|Cornelis T. Verrips, Adrianus M. Ledeboer, Luppo Edens, Robert Klok, Jan Maat
|Original Assignee
|Unilever Patent Holdings B.V.
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (4), Non-Patent Citations (57), Referenced by (6), Classifications (24), Legal Events (5)
|External Links: USPTO, USPTO Assignment, Espacenet
This is a continuation of application Ser. No. 329,829, filed Dec. 11, 1981, which was abandoned upon the filing hereof.
The present invention relates to DNA sequences encoding the various allelic forms of mature thaumatin, and cloning vehicles comprising said DNA sequences and their use in transforming microorganisms.
Thaumatin is a protein originating from the arils of the fruit of Thaumatococcus daniellii. Thaumatin is, on a weight basis, 1600 times sweeter than sucrose and on a molecular basis 105 times sweeter than sucrose. In Western society overconsumption of sugar causes a number of health problems. Therefore, many attempts have been made to substitute low caloric sweeteners for sugar. However, several of these have recently been prohibited in view of possible side-effects. There is thus a need for a natural low caloric sweetener and for an economical process of producing such a sweetener. Recent advances in molecular biology have enabled the introduction of structural genes coding for specific eukaryotic proteins into microbial host cells and expressing said genes in the transformed host cells, thereby producing the desired protein.
Many genes of eukaryotic origin which in their natural state encode proteins in their unprocessed forms cannot be applied directly in recombinant DNA molecules because natural genes contain DNA sequences called introns, which are not contained in the messenger RNA (mRNA). The information located on these introns is removed in eukaryotic cells before the translation process of the mRNA. As far as Applicants are aware, bacteria are unable to excise such introns at RNA level and therefore it is necessary to remove the genetic information located on these introns at RNA level before the natural gene of eukaryotes can be used in prokaryotic host cells.
In microbial host cells, that have the capability of excising introns at mRNA level the natural genes can in principle be applied, provided that they are brought under control of regulons that are effective in said microbial host cells.
In the present invention use is made of recombinant DNA techniques that introduce the genetic information of eukaryotic, particularly of plant, origin, in such a state that expression occurs effectively in microbial, particularly in bacterial host cells.
For a better understanding of the invention the most important terms used in the description will be defined:
A regulon is a DNA sequence consisting of a promotor and operator region.
Structural genes are DNA sequences which encode through a template (mRNA) a sequence of amino acid characteristic of a specific polypeptide.
A promoter is a DNA sequence within the regulon to which RNA polymerase binds for the initiation of the transcription.
An operator is a DNA sequence within the regulon to which a repressor protein may bind, thus preventing RNA polymerase from binding to the adjacent promotor.
An inducer is a substance which deactivates a repressor protein, freeing the operator and permitting RNA polymerase to bind to the promoter and start transcription.
By mature thaumatin is meant one of the allelic forms of the fully processed protein (FIG. 1).
Cloning vehicle. A non-chromosomal double-stranded DNA, plasmid or phage, comprising a DNA sequence (intact replicon) that allows self-replication after transformation into suitable host cells.
Phage or bacteriophage. Bacterial virus which can replicate in a suitable bacterial host cell.
Reading frame. The grouping of triplets of nucleotides (codons) into such a frame that at mRNA leve a proper translation of the codons into the polypeptide takes place.
Transcription. The process of producing RNA from a gene.
Translation. The process of producing a polypeptide from mRNA.
Expression. The process undergone by a structural gene to produce a polypeptide. It is a combination of many processes, including at least transcription and translation.
By mature thaumatin gene is meant the double-stranded DNA sequence having exactly the same information content (sequence of codons) as that part of the messenger RNAs coding for the various allelic forms of thaumatin in their fully processed (mature) form. For reasons of convenience only one strand of ds DNA is given in the text and figures.
According to the invention there is provided a recombinant plasmid comprising:
(i) structural genes coding for the various allelic forms of thaumatin and particularly mature thaumatin, according to FIGS. 1 and 2
(ii) specific DNA sequences which regulate the expression of said structural genes. These specific DNA sequences consist of either an inducible or a constitutive regulon. A preferred inducible regulon consists of a double lac UV5 system as described by D. V. Goeddel et al., Nature 281, 544-548 (1979).
Another preferred inducible regulon is a constituent of the tryptophan system described by F. Lee et al., J. Mol. Biol. 121, 193-217 (1978) and K. Bertrand et al., Science 189, 22-26 (1975). Applicants have modified this tryptophan system to obtain a more adequate system according to FIG. 3. In this modified system the information coding for the trp attenuator protein is eliminated while maintaining the ribosome binding site.
The recombinant plasmid according to the invention may comprise DNA sequences consisting of a modified promoter/ribosome-binding site of gene VIII of bacteriophage M13, fd of fl [P. M. G. F. van Wezenbeek et al., Gene 11, 129-148 (1980)], which, as far as Applicants are aware, were never used before for the expression of eukaryotic genes.
In the recombinant plasmid according to the invention the regulon may be either directly linked to the structural gene or indirectly through a novel start codon and EcoRI-site containing DNA linker comprising the nucleotide sequence CAT(N)n GAATTC(N')n ATG, wherein n=0, 1, 2 or 3, and N and N' are any of the nucleotides A, T, G or C, with the proviso that in the double-stranded structure N and N' are such that a rotational symmetrical structure is present.
By a rotational symmetrical structure is meant that where N is e.g. represented by A, N' should be represented by the complementary base T.
In some instances it turned out that the yield of expression improved when the sequence AATT between the regulon and the structural gene has been eliminated.
The microbial cloning vehicles containing the structural genes encoding the various allelic forms of the mature (fully processed) thaumatin according to the invention are obtained and the various thaumatins are produced by performing a number of steps, the most essential of which are:
(1) isolation and purification of the messenger RNA (mRNA) of thaumatin;
(2) conversion of this mRNA into double-stranded DNA (ds DNA);
(3) construction of ds DNA having a poly-dC tail;
(4) incorporation of the ds DNA-poly-dC molecules in endonuclease PstI-cleaved, and poly-dG-tailed plasmid pBR 322 DNA;
(5) transformation and clone selection;
(6) determination of the nature of the insert by RNA/DNA hybridization and in vitro translation;
(7) double-checking the nature of the inserts by DNA- and RNA-sequence analysis;
(8) producing DNA encoding the mature fully processed thaumatin;
(9) construction of plasmids comprising specific transcription regulating DNA sequences, and chemical synthesis of DNA-linkers and -primers;
(10) construction of plasmids comprising a constitutive or inducible regulon and the ligated thaumatin gene; and transformation of E.coli with said plasmids;
(11) culturing of E.coli cells containing said recombinant plasmids and detection and isolation of the thaumatin.
The following detailed description will illustrate the invention.
1. Isolation and purification of mRNA (thaumatin)
Isolated arils of Thaumatococcus, daniellii were ground under liquid nitrogen. After protein extraction with phenol, a selective precipitation of the RNAs with LiCl was performed following the procedures described by K. S. Kirby (1965) Biochem. J. 96, 266-269, U. Wiegers and H. Hilz (1972) FEBS Letters 23, 77-82. Poly-A containing messenger RNA was recovered by several passages over oligo-dT-cellulose columns and from this messenger mixture the thaumatin-encoding mRNA was isolated by polyacrylamide gel electrophoresis. This was checked by translation of the mRNA in the wheat germ system as described by H. Aviv and P. Leder (1972), Proc. Natl. Acad. Sci. U.S.A. 69, 1408-1412 and J. W. Davies and P. Kaesberg (1973), J. Virol. 12, 1434-1441.
2. Conversion of thaumatin mRNA into double-stranded DNA
The purified thaumatin mRNA was copied with AMV reverse transcriptase to yield a single-stranded DNA molecule, according to the procedure described by G. N. Buel et al., J. Biol. Chem. 253, 2471-2482 (1978). This cDNA subsequently converted into a double-stranded molecule by E.coli DNA-polymerase, according to the procedure described by A. R. Davis et al., Gene 10, 205-218 (1980). The loop structure of the double-stranded DNA copy was removed by S1 -nuclease digestion.
3. Construction of double-stranded DNA with poly-dC tails
DNA molecules of the desired length were obtained by polyacrylamide gel-electrophoresis, extracted from the gel and tailed with poly-dC by terminal transferase according to the procedure described by R. Roychoudhury et al., Nucleic Acids Research 3, 863-877 (1976).
4. Integration of the ds DNA-dC molecules in plasmid pBR 322
Plasmid pBR 322 was treated with restriction endonuclease Pst I, that cleaves the plasmid at a recognition site that lies in the β-lactamase gene, whereafter the linearized DNA of pBR 322 was supplied with poly-dG tails by terminal transferase. The poly-dC tailed DNA molecules were annealed to the poly-dG tailed plasmid pBR 322.
5. Transformation and clone selection
The plasmids thus obtained were transferred into CaCl2 -treated E.coli cells. After transformation cells containing hybrid plasmid DNA molecules were selected on their resistance to tetracycline. Positive colonies were screened for plasmids with large inserts by a combination of a rapid plasmid extraction procedure as outlined by H. C. Birnboim and J. Doly, Nucleic Acids Research, 7, 1513-1523 (1979) and an endonuclease Pst-I-digestion of the isolated DNA.
6. Determination of the nature of the inserts (I). Hybridization/in vitro translation.
From the selected clones 10 μg plasmid DNA were isolated, which subsequently were bound to diazotated (DMB) paper discs. The immobilized plasmid DNA molecules were used in an hybridization/in vitro translation procedure as outlined by J. G. Williams et al., Cell 17, 903-913 (1979) in order to determine the nature of the DNA insert.
7. Determination of the nature of the inserts (II) by DNA/RNA sequence analysis
The nucleotide sequence analysis of the thaumatin inserts was performed by the chemical degradation procedure as outlined by A. M. Maxam and W. Gilbert in Methods in Enzymology, L. Grossman and K. Moldave editors, New York, Acad. Press, 1980, Vol. 65 (1), pages 499-560 and by the dideoxy/nick translation procedure as outlined by J. Maat and A. J. H. Smith, Nucleic Acids Research, 5, 4537-4545 (1978). Further information on the nucleotide sequence of the thaumatin mRNA was derived indirectly by primed synthesis by AMV-reverse transcriptase on the thaumatin mRNA template in the presence of chain terminating inhibitors, as outlined by D. Zimmern and P. Kaesberg, Proc. Natl. Acad. Sci. U.S.A. 75, 4257-4261 (1978). This screening yielded inter alia plasmid pUR 100 containing an almost complete copy of thaumatin mRNA.
8. Construction of DNA encoding the mature, fully processed thaumatin
Single-stranded DNA was obtained by exonuclease III treatment of pUR 100 as outlined by A. J. H. Smith, Nucleic Acids Res., 6, 831-848 (1979) or by cloning in M13, as outlined by B. Gronenborn and J. Messing, Nature 272, 375-377 (1978). Single-stranded DNA with the same polarity as the thaumatin mRNA was used as template for complementary DNA synthesis with the chemically synthesized oligonucleotide (5') pTCAGGCAGTAGGGCAOH (3') serving as a primer; after heat-denaturation of the double-stranded DNA, the complementary DNA served as template for DNA synthesis by using the chemically synthesized oligonucleotide (5') pGCCACCTTCGAGOH (3') as a primer. This double-stranded DNA was then treated with S1 nuclease. The construction of the mature thaumatin gene is illustrated in FIG. 4.
9a. Construction of a plasmid pUR 201
A fragment containing 285 base pairs comprising the double lac regulon (lac UV5) was obtained by restriction endonuclease EcoRI cleavage of pKB 26B, (K. Backman and M. Ptashne, Cell 13, 65-71 (1978)). This fragment was ligated in the EcoRI site of pBR 322 DNA. Plasmid DNA with the lac regulon in the right orientation (FIG. 5) was partly cleaved by EcoRI in the presence of E.coli RNA polymerase. The EcoRI cleavage site most distant from the restriction endonuclease Hind III cleavage site was preferentially attacked. The linearized DNA was treated with S1 nuclease, purified by agarose gel electrophoresis, circularized by ligation with T4 DNA-ligase and subsequently used to transform E.coli. From the tetracycline-resistant transformants pUR 201 with the correct structure (FIG. 5) was obtained.
9b. Construction of plasmid pUR 301
A DNA fragments of about 510 base pairs was obtained by restriction endonuclease Hinf I cleavage of ptrp ED5, (R. A. Hallewell and S. Emtage, Gene 9, 27-47 (1980)). This fragment was cleaved with restriction endonuclease Taq I in the presence of E.coli RNA polymerase. The Taq I site in the trp regulon (described by K. Bertrand et al., Sience 189, 22-26 (1975) and F. Lee et al., J. Mol. Biol. 121, 193-217 (1978)) was selectively protected, thus yielding a fragment containing 234 base pairs comprising the trp regulon (FIG. 3). This fragment was then treated with S1 nuclease, blunt-end ligated with the EcoRI-linker (5') pGGAATTCCOH (3'), cut with EcoRI and subsequently cloned in the EcoRI-site of pBR 322.
Plasmid pUR 300 with the trp regulon in the correct orientation (FIG. 3) was isolated. The EcoRI-cleavage site most distant from the Hind III site was removed by partial cleavage of pUR 300 DNA by EcoRI in the presence of ethidium bromide, and S1 nuclease treatment. Linear DNA molecules were recircularized by T4 DNA ligase. From the tetracycline-resistant transformants pUR 301 with the structure as outlined in FIG. 3 was obtained.
9c. Construction of Plasmid pUR 401
A fragment containing 270 base pairs (DNA sequence 1128-1397) was obtained by digestion of RF M13 DNA (see P. M. G. F. v. Wezenbeek et al., Gene 11, 129-148 (1980)), with the restriction endonuclease Taq I and Hae III and the Taq I site was made blunt-ended by a repair reaction with E.coli DNA polymerase; the fragment was subsequently partly digested with restriction enzyme MnlI. The partial products were treated with successive actions of T4 DNA polymerase and S1 nuclease and subsequently blunt-end ligated with the EcoRI-linker (5') pGGAATTCCHOH (3'), then treated with EcoRI and ligated in the EcoRI site of the pBR 322. By restriction enzyme analysis and DNA sequence analysis a plasmid was obtained in which the EcoRI cleavage site was located just beyond the ribosome-binding site of the M13 gene VIII DNA sequence. Applicants have found that the plasmids having the M13 regulon from nucleotide 1128 to nucleotides 1291 to 1297 were appropriate regulons for expression. The EcoRI cleavage site most distant from the Hind III site was removed essentially as described for pUR 301. The complete construction of pUR 401 is outlined in FIG. 6.
9d. Chemical synthesis of linkers and primers
The synthesis of oligodeoxynucleotides is carried out through coupling of 5'-0-levulinyldeoxynucleoside-3'-0-2,2,2-trichloroethyl-2-chlorophenyl phosphates with deoxynucleoside-3'-0-2,2,2-trichloroethyl-2-chlorophenyl phosphates. This method, which is known as the phosphotriester method (described by J. F. M. de Rooij et al., Recl. Trav. Chim. Pays-Bas 98, 537-548 (1979)), involves splitting off the trichloroethyl group by active zinc, followed by the actual coupling reaction with the help of 2,4,6-triisopropylbenzenesulphonyl-3-nitro-1,2,4-triazole. The amino groups in deoxyadenosine, deoxycytidine and deoxyguanosine are protected by a benzoyl group, a 4-methoxybenzoyl group and a benzoyl group, respectively. For the protection of the 3'-hydroxy function of the terminal nucleoside the benzoyl group is used. In the last step all protecting groups are removed through reaction with tetrabutylammonium floride and concentrated aqueous ammonia, respectively.
An example of the construction of the linker (5') pCATGAATTCATGOH (3') is given in FIG. 7.
10. Construction of expression plasmids with the mature thaumatin gene under transcription control of the double lac UV5-(pUR 520), the trp-(pUR 530) and the M13 or f1 or fd gene VIII region (pUR 540) and transformation of E. coli with said plasmids.
The thaumatin encoding DNA fragment described under 8 was blunt-end ligated with the synthetic EcoRI-linker (5') pCAT(N)n GAATTC(N')n ATGOH (3'), n being zero, with T4 DNA ligase, cleaved with EcoRI and subsequently ligated in the EcoRI-cleavage site of the plasmids pUR 201, pUR 301 and pUR 401, and recombinant plasmids with the thaumatin encoding insert in the orientation as illustrated in FIG. 8 were isolated after transformation of E. coli and selection of tetracycline-resistant transformants. In the above-described plasmids the AATT sequence originating from the chemically synthesized linkers could be deleted by cleavage of the plasmids with EcoRI in the presence of ethidium bromide; linear partials were isolated by agarose gel electrophoresis, treated with S1 nuclease and recircularized by T4 DNA ligase action.
Plasmids obtained after deletion of AATT were detected by restriction enzyme analysis.
11. Culturing of E. coli cells containing said recomnant plasmids and detection of the thaumatin
E. coli cells containing plasmids pUR 520 or pUR 530 or pUR 540 with or without the AATT sequence in the linker between the regulon and the mature thaumatin gene(s) in the correct orientation and reading frame were cultured under conditions most suitable for their growth--these culturing conditions vary with the type of plasmid present in the cells--but always in the presence of an appropriate anti-biotic to maintain selection pressure. Under these conditions the cells containing either plasmids pUR 520 or pUR 530 or pUR 540 produced considerable amounts of mature thaumatin.
The presence of the protein thaumatin was demonstrated qualitively by S.D.S. electrophoresis and by physiological tests on their sweetness and quantitatively by the enzyme linked immuno sorbent assay (ELISA).
Cells of E. coli strains K12(294) containing plasmids pUR 520 or pUR 530 were deposited under the Budapest Treaty on Dec. 2, 1981, at the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md., U.S.A., and have acquired the following registration numbers: ATCC 39014 and ATCC 39013, respectively.
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|Board Of Trustees Of The Leland Stanford Jr. University
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|Research Corporation
|Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules
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|Citing Patent
|Filing date
|Publication date
|May 2, 1988
|Nov 7, 1995
|Xoma Corporation
|DNA encoding (ASP 113) and (LYS 46, ASP 113) thaumatin I
|Jun 21, 1996
|Jan 12, 1999
|University Of Hawaii
|Recombinant sweet protein mabinlin
|Apr 21, 1995
|Aug 3, 1999
|Urquima, S.A.
|Preparation of thaumatin sweeteners
|May 20, 1998
|Apr 18, 2000
|University Of Hawaii
|Recombinant sweet protein mabinlin
|Oct 15, 2003
|May 24, 2011
|Shemyakin And Ovchinnikov Institute Of Bioorganic Chemistry Russian Academy Of Sciences
|Method for producing a transgenic plant with the aid of Agrobacterium thumefaciens
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|Method for producing a transgenic plant with the aid of agrobacterium thumefaciens
|U.S. Classification
|435/69.1, 435/488, 435/320.1, 435/91.41, 536/23.6, 435/252.33
|International Classification
|Cooperative Classification
|European Classification
|Apr 28, 1987
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Beats Music, the latest online venture to enter the highly competitive music subscription business, announced on Wednesday that it plans to debut it's service in January 2014. Following the remarkable success of his Beats By Dre headphone line, Dr. Dre, along with Jimmy Iovine and new Beats Music CEO Ian Rodgers have created a service tailored toward artist-recommended playlists and suggestions, unlike Spotify which recommends music based on algorithmic formulas. Former Nine Inch Nails front man Trent Reznor, appointed Chief Creative Officer on the project, describes it as "…having your own guy when you go into the record store, who knows what you like but can also point you down some paths you wouldn't necessarily have encountered."
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Halyna's place was the perfect location during our stay in Lilooet. It comfortably accommodated the 5 of us and was well set up for us to cook and relax after a long drive. Halyna had left a delicious breakfast of eggs, bread, yogurt and granola for us in the fridge and she was on hand to help us when we first arrived. I would have no hesitation in recommending Halyna's B&B for anyone looking for a base during their stay in Lilooet. Thanks again Halyna!Philippa2016-09-12T00:00:00Z
The apartment was handy, clean and as described. There are amazing views from the garden over the valley. Check-in was easy and we found everything we needed. There are restaurants nearby to eat in the evening and a bakery for snacks open during the day, all a few minutes walk away. Thanks for a comfortable nights stay and the homemade breakfast!Alice2016-08-24T00:00:00Z
Halyna was friendly and made us a delicious breakfast.Linda2016-07-25T00:00:00Z
A large clean apartment with lots of equipment. Very helpful and accommodating host.Kate2016-08-10T00:00:00Z
Lovely house with a lovely view. Halyna is a very nice host with delicious breakfastsStuart2016-06-27T00:00:00Z
After a week of staying in motels, we spent a comfortable night in this spacious apartment. Halyna was very flexible with our booking (a storm was coming and when booking our stay we weren't sure we would make it to Lillooet that day but fortunately we did). Everything was as advertised. Couldn't have been happier.Teemu2016-10-16T00:00:00Z
Very clean and inviting. Thank you, Halyna for a great stay. Your breakfast was delicious!Khai & Steve2016-08-06T00:00:00Z
Our stay with Halyna was great. The perfect place to explore Lillooet. Highly recommend this space!York by the beach2016-10-02T00:00:00Z
Art der Unterkunft
Ganze Unterkunft
Unterkunft für
Lillooet, British Columbia, Kanada
We really enjoyed our weekend in d'Arcy area. The apartment was as advised: clean, nice and cosy. Romy was very kind and was there to welcome us at our arrival. Thanks!!Amélie2016-01-24T00:00:00Z
Shiloh Works is a fantastic place- wonderfully peaceful with great facilities. Beautiful countryside and hiking in the area. This place is a gem!Ed2016-07-29T00:00:00Z
We had a great time! Really enjoyed the comfy, well appointed apartment and the beautiful scenery. Highly recommended!Allison2016-02-14T00:00:00Z
The hot tub with the amazing view!! They also have an awesome shared rec room with lots of entertainment. It's a really nice place to stay. Romy and Dani are super friendly and they respond to your message very quickly.Christia2016-12-29T00:00:00Z
Very clean and cozy thanks for hosting us , we enjoyedGlenn2016-02-13T00:00:00Z
Romi and Dani welcome us very well even if we arrived at souper time. Their little farm is very cute and I think it's a great place for kidsWilliam2016-08-20T00:00:00Z
great stay ! beautiful area... thank you !Simon2016-06-20T00:00:00Z
A little bit out of the way but central enough for most day trips. Facility was clean and comfortable and a good price for a large group.Miles2017-02-20T00:00:00Z
Art der Unterkunft
Ganze Unterkunft
Unterkunft für
D'Arcy, British Columbia, Kanada
The house has an absolutely stunning view, with a huge rec room with a pool table, Foosball table, and sound system that can easily accommodate 10+ people at a time. Romi and her family was very welcoming and helpful in our stay, including some wood chopping tips that came in handy. The living area unfortunately on had one bathroom, which meant we had to do a bit of planning for shower time, but it was not too much hassle compared to what was offered. Definitely recommend for a group getaway!Simon2015-12-24T00:00:00Z
Perfect for an incredible getaway with friends! It has every ingredient for a beautiful, relaxing but still fun-filled weekend away from the city!Danae 2017-03-12T00:00:00Z
Vi blev modtaget af en hjertelig og venlig Romy, som viste os tilrette og gav os kort og info om steder vi kunne vandre i nabolaget. Lejligheden var ren og pæn og alt var velfungerende. Dog gik strømmen en enkelt gang, men Romy var straks på pletten med hjælp. Stedet har en hyggelig frugthave med udsigt til bjergene. Her kan man slappe af i fred og ro.Else2015-08-23T00:00:00Z
I planned a birthday trip for my girlfriend inviting her close friends to Whistler and I couldn't have done it if it wasn't for this place. The place had everything we needed. It was clean, the kitchen was fully equipped, and had plenty of room for everyone. The hot tub was amazing!! Thank you Romy and Dani for being such a wonderful host!!Andrew2015-12-31T00:00:00Z
We enjoyed staying at the Shiloh-Works. The private suite for 8 was clean, comfy and quiet. Nothing too fancy, but exactly what you need for a getaway. The orchard surrounding was beautiful and so were the views. We had a great time spending a birthday weekend there with 7 people and 2 dogs.Lena2014-08-31T00:00:00Z
It's a great place for family and kids. It's so beautiful and peaceful there. The pool, hot tub and trampoline provide the kids with countless hours of fun. The accommodation is basic but perfectly functional and clean. Romy is also such a warm and friendly host.Yeng2016-08-15T00:00:00Z
Wonderful place, stunning location! Great place for anyone looking to be out of the city and close to lots of great nature to explore!Bryan2017-01-08T00:00:00Z
Excellent weekend getaway! Felt secluded up in the beautiful mountains. We stayed as a group of 8 and the hosts were friendly and very accommodating! Loved the animals on the property.Shannon2016-08-22T00:00:00Z
Art der Unterkunft
Ganze Unterkunft
Unterkunft für
Poole Creek, British Columbia, Kanada