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Use of Solvent Iodide Ions as an Effective In-house Tool for Crystallographic Phasing Michael R. Sawaya & Duilio Cascio January 17, 2002
Phasing by solvent halide ions promises to be a revolutionary new way to phase protein structures Quick soak in halide (30 seconds) Easy to perform Applicable to any protein Non-toxic, no heavy atom waste High quality phasing Acta Cryst (2000). D56, 232-237
Iodide is the choice of halide for in-house phasing Atomf" I-6.9 Pt7.0 Br-1.3 C0.01 N0.02 O0.03 S0.56 Anomalous scattering correction factors at CuK wavelength At CuK , f" is comparable to Pt, Hg, Au 54 electrons F-RD generator high flux, fine focus
How successful is phasing by iodide at UCLA? Original study performed with only 4 proteins using a synchrotron source. Will this method prove to be generally applicable in a real academic research lab with real proteins with real problems? What are the limitations? (e.g. resolution limits, lack of isomorphism, data quality, soaking conditions, number of iodide sites required for good phases) Will all proteins bind a sufficient number of iodides to generate good phases? Dauter et al., suggest that the number of iodides bound is simply proportional to the surface area of the protein. Is this always true?
Students, Post docs, and Staff from MBI generously donated their crystals to test the effectiveness of iodide soaks in phasing protein structure proteincontributor Rv1926cCelia Goulding Rv3697cCelia Goulding Rv2878cCelia Goulding DsbD-N-termCelia Goulding P51Chongwoo Kim SmAPCameron Mura NarLc complexAnn Maris RNase ds-trimerYanshun Liu Protein isoaspartyl methyl transferase Scott Griffith Daniel Boutz MyoglobinMaria Grzeskowiak Proteinase KHelty Adisetiyo ThaumatinHelty Adisetiyo XylanaseHelty Adisetiyo lysozymeStudents of M230B (2001) Thank You for the crystals!
Experimental methods flow chart SOAKING PROCEDURES Weigh 0.008g KI (one medium sized grain) Dissolve KI in 100 uL of reservoir solution Add appropriate % of glycerol (for cryoprotection) Soak crystal 30 seconds Mount on cold stream DATA COLLECTION PROCEDURES Collect data on F-RD when possible Collect 360 degrees of data Process with Denzo/Scalepack DETERMINE IODIDE SUBSTRUCTURE Import data with xprepx (Bruker) Calculate difference Patterson coefficients using SAS, SIRAS, SIR data Locate Iodide sites with ShelxD Verify quality of sites by overlapping predicted Patterson peaks on Patterson map PHASING CCP4 suite: scalepack2mtz, truncate, cad, scaleit, mlphare, dm MODEL BUILDING Arp/wArp
12/14 crystals soaked showed clear evidence of iodide binding
8/14 Iodide soaks led to complete structure determination 1 structure had not been previously determined
Example of electron density generated by SIRAS phasing based on 11 iodide sites DsbD N-term
Table 1. Data Statistics on Iodide Soaked Crystals Summary: Iodide is my first choice for derivatization. No other heavy atom as successful with so many proteins under so many different conditions Eight structures solved using phases based on iodide Clear iodide sites but poor phasing Non- Isomorph ous 3 13% 1.8A
Tips for successful phasing with iodide Poor peak heights in difference Patterson map? High redundancy of intensity measurements is crucial to locating heavy atom sites and phasing. Collect 360 degrees of data. Not just iodide, but any derivative would benefit. Iodide soak is non isomorphous with native? Non- isomorphorism can be reduced by a quick back-soak in cryo- conditions lacking iodide. (eg. Rv2878c) Iodide sites not convincing? ShelxD often succeeds at finding iodide sites based on anomalous differences alone. But, If the solution is not clear, try using isomorphous differences (SIR) or a combination of isomorphous and anomalous differences (SIRAS) output by xprepx.
Data collected using FR-D generator can produce better quality maps than RU200 generator I/ (2.0 A) 7.6 26.4 R sym (2.0 A) 37.3% 7.1%
Poor phasing is a direct consequence of too few iodides/surface area Need 1 iodide bound per 10-20 residues
Why do some proteins bind disproportionately fewer iodides/surface area? Two possibilities 1)Soaking conditions (e.g. pH, salt, buffer) disfavor or compete with iodide binding. If true then we could search for conditions that favor iodide binding. or 2) Residue composition of the protein surface disfavors iodide binding. Make predictions about iodide binding based on amino acid composition.
Iodide binding appears insensitive to the composition of the cryo-solvent Thaumatin 1.3M Na,K tartrate 35% glycerol Bis-Tris pH 6.5 0.5M KI 1 iodide/14 residues Rv1926c 0.1M (NH 4 ) 2 SO 4 30% PEG 4000 Tris pH 7.0 0.5M KI 1 iodide/47 residues Soaking a Rv1926c crystal in thaumatin's cryo-conditions did not increase the number of iodides bound. But, why expect conditions that are optimal for iodide binding to one protein to also be optimal for another protein? Thaumatin is a more basic protein (pI=8.5) than Rv1926c (pI=6.1). Perhaps if I tried a more substantial change in pH to change the electrostatic potential of the surface… Experiment to test effects of cryo-solvent on iodide substitution
Higher pH appears to weaken iodide binding Proteinase K 0.1M (NH 4 ) 2 SO 4 30% glycerol Cacodylate pH 6.5 0.5M KI Proteinase K 20% PEG 8000 20% glycerol CHES pH 9.5 0.5M KI Top 3 negative peaks in F obs(pH9.5) –F obs(pH6.5) difference Fourier map correspond to iodide sites. Experiment to test effects of pH on iodide substitution
Tally of side chains in contact with 102 iodide sites Note: Arginine and lysine are the two residues most frequently found in iodide binding sites.
The amino acid composition favored by iodide is significantly depleted in negatively charged side chains compared to the average amino acid composition on the surface of most proteins Red data points taken from The Atomic Structure of Protein-Protein Recognition Sites by Lo Conte, Chothia & Janin, J. Mol. Biol., 285,2177-2198 CC=0.77
Table 1. Data Statistics on Iodide Soaked Crystals Most successful iodide experiments were conducted at a pH below the pI with the exception of Rv2878c 3 13% 1.8A >>>>><>><<<<>>>>><>><<<<
A protein may still bind iodide even if pH > pI since iodide binding sites are often non-polar No consensus coordination geometry Polar & nonpolar Hydrogens at a radius 3.5-4 Angstroms Peptide planes Could involve any of the 20 amino acids
Conclusions SIRAS phasing from iodide soaks in-house is effective, quick, easy, and non-toxic. 8/14 structures could be determined at UCLA Even in cases where there are too few iodide sites to produce a good map, iodide sites could be used in combination with other derivatives (e.g. CsCl). High redundancy, high resolution, and a bright, focused x-ray source (F-RD) are important factors for success. Soaking at pH < pI improves chances of success Future: Lower the pH of cryo-conditions of Rv1926c or xylanase to increase iodide binding and solve another structure.
Acknowledgements Duilio Cascio- partner in experiments, advice, inspiration CRYSTALS Celia Goulding Chongwoo Kim Cam Mura Ann Maris Yanshun Liu Scott Griffith Daniel Boutz Maria Grzeskowiak Helty Adisetiyo SUPPORT David Eisenberg Todd Yeates Richard Dickerson James Bowie Zbigniew Dauter-advice on back-soaking, shelxD, xprepx. Peter Muller-xprep connections Kim Ma –X-ray maintenance STATISTICS Gary Kleiger Todd Norcross
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Gourmet cuisine provides insight as to what each culture considers the art of good living. For some cultures it's luxurious and expensive, for others it's sophisticated and rare. Conduct a search for the best gourmet restaurant in your city and enjoy!
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Le Vieux LogisLe Bourg 24510 Trémolat
Average price €73.00"Menu "Balade EstTaxesle" Accord Mands and Vins 105€"
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La MetairieMauzac 24150 Mauzac-et-Grand-Castang
Average price €38.00
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Restaurant Les Merles - Château Les MerlesTuilières 24520 Mouleydier
Average price €44.00
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Nearby cities
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Near Kennesaw in Cobb County, Georgia — The American South (South Atlantic)
Durham House
Baird's, together with 1st and 2d divisions, were camped along Proctor's Cr., E. - a part of the concerted drive on Johnston's forces [CS] near Kennesaw. Heavy rains halted military movements for several days in this area.
Oct. 9. Baird revisted Durham house enroute to Gaylesville, Ala. in pursuit of Hood's forces [CS] withdrawing to Tennessee.
Erected 1953 by Georgia Historical Commission. (Marker Number 033-27.)
Marker series. This marker is included in the Georgia Historical Society/Commission marker series.
Location. 34° 1.097′ N, 84° 40.53′ W. Marker is near Kennesaw, Georgia, in Cobb County. Marker is on Acworth-Due West Road 0.2 miles north of Mars Hill Road, on the left when traveling north. Click for map. Marker is in this post office area: Kennesaw GA 30152, United States of America.
Other nearby markers. At least 8 other markers are within 3 miles of this marker, measured as the crow flies. Route of Polk's Corps (approx. 0.4 miles away); Peters-Davenport House (approx. 1.3 miles away); Stilesboro-Sandtown Crossroads (approx. 1.4 miles away); Site ~ Mason's Bridge (approx. 1.9 miles away); Site of Old Allatoona Church (approx. 2.3 miles away); General Leonidas Polk Memorial (approx. 2.6 miles away); Pine Mountain (approx. 2.7 miles away); Lt. General Leonidas Polk Killed at Pine Mountain (approx. 2.7 miles away). Click for a list of all markers in Kennesaw.
Regarding Durham House. The Durham House is no longer standing.
Categories. • War, US Civil •
Credits. This page originally submitted on , by David Seibert of Sandy Springs, Georgia. This page has been viewed 888 times since then and 17 times this year. Photo 1. submitted on , by David Seibert of Sandy Springs, Georgia. • Craig Swain was the editor who published this page. This page was last revised on June 16, 2016.


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Crazy Creatives Lead
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And this is not the actor's first film..
She could not have thanked the leads enough!
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Most interestingly, Zion Train (right) are perhaps symbolic of the fracturing of the Left consensus over the last 10 years. The Labour Party acquired corporate-businessmen smiles and lurched to the Right, while the under- 30s grasped counter-culture lifestyles, became everything from rave organisers to eco-warriors to cyber-industry anarchists. When the election comes around, the Labour Party makes young people some tantalising offers: 20- year student loans, the possibility of 10pm curfews and workfare in place of benefits. Once upon a time, Left dwellers like Zion Train would have lent full support. Nowadays, they will probably be otherwise engaged. This summer, doubtless, they'll be in a festival field near you.
Zion Train, Manchester Nia Centre (0161-227 9254) tomorrow; Plymouth Cooperage (01752 229 275) 10 Jun; Exeter Cavern (01392 495 370) 11 Jun; Leicester University (0116-255 5166) 13 JunReuse content


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Reminder: Physicals must be completed prior to practicing or trying out. Available: High School Office, Memphis Website, and on MHSAA.com
When school is canceled there will be no Junior High Athletics. High School Athletics will be decided on a day to day basis with every attempt to make the decision by noon.
If you are interested in being on a committee to discuss Football 2017, please e-mail Mr. Kujat @ firstname.lastname@example.com by Nov. 3rd, 2016.


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Foursquare © 2017 Lovingly made in NYC & SF
Lawlors Hotel
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Make sure your information is up to date. Plus use our free tools to find new customers.
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Use keyboard shortcuts to capture on-screen information
You can use keyboard shortcuts to digitally capture on-screen information such as a scanned image of a check that might appear on your financial institution's website.
For example, you can press ALT+PRINT SCREEN to make a copy of the active window, or press PRINT SCREEN to copy the entire screen as it appears on your monitor. This places the image in the Microsoft® Windows Clipboard. You can then use the Clipboard button in the Attachments Viewer to attach the image file to a Quicken transaction.


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A polio vaccine that has been given to millions of children and adults was withdrawn by the Government yesterday amid fears it could be contaminated by mad cow disease.
A polio vaccine that has been given to millions of children and adults was withdrawn by the Government yesterday amid fears it could be contaminated by mad cow disease.
The Medical Control Agency ordered GPs to return all unused doses after the finding that it had been produced using foetal calf serum from the UK.
Guidelines in 1989 banned the use of bovine material in medicinal products, but despite repeat assurances from Medeva, the maker, the Department of Health found it was in breach of the guidelines.
The company, which has made one-third of polio vaccines used in Britain since the early 1980s, stopped producing the vaccine last month.
Dr Peter Fellner, chief executive of Celltech, which bought Medeva from Wellcome and owned it from January to October this year, said records relating to the batch of vaccines in question did not "fully clarify" its BSE-free status. "We had assurances from Wellcome that there were no issues over the vaccine and passed on these assurances to the Department of Health. What Wellcome told us was found not to be accurate," he said.
Professor Liam Donaldson, the chief medical officer, said the risk of anyone being infected with vCJD, the human form of BSE, was "incalculably small". The recall comes as it was announced that a report into Britain's BSE crisis will be published next week after a 30-month inquiry.
Medical experts urged parents to continue getting their children immunised, warning that any fall in vaccination levels could result in polio returning to this country for the first time in decades. Worried parents and patients have been advised to see their GPs or contact NHS Direct.
Despite the obvious breach of the guidelines, which were updated in 1999, no action can be taken against any company as the guidance does not become legally binding until next year when it will be covered by an European Union directive.
Andrew Kemp, chief executive of the British Polio Fellowship, which supports victims, said: "What we would hope is that this does not deter anyone from being vaccinated. The vaccine has been extremely successful in combating polio."
Liam Fox, the shadow Health Secretary, said: "The immunisation programme is one of the most important parts of public health policy... It is therefore essential that all information is fully disclosed by the Government to help retain the confidence of an understandably sceptical public."Reuse content


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Martina McBride Hosts Baby Shower for Homeless Young Women
Martina McBride, along with her charitable initiative Team Music Is Love, recently carried out an extra special day for some New York City mothers and mothers-to-be.
Martina McBride, along with her charitable initiative Team Music Is Love, recently carried out an extra special day for some New York City mothers and mothers-to-be.
Martina McBride fronted the lineup for CMT's Next Women of Country Tour, with special guests Lauren Alaina, Post Monroe and Maggie Rose. The tour made a stop in the heart of New York City's Times Square, and McBride led a fully girl-powered show just in time for Women's Day, with Taste of Country on hand to capture some exclusive concert pictures.
These 20 country music albums are turning 20 years old in 2017.
There are few things more adorable than a baby bump.
Martina McBride who has a mega voice will be in concert in Lake Charles in less than two weeks on January 14th of 2017.
Martina McBride, one of Country music's best singers ever, will be in concert on January 14th of 2017 in Lake Charles.
Martina McBride has been around the block in country music, watching the trends ebb and flow, and she agrees with other artists that songwriting in country music has fallen into a predictable pattern over the last few years. But she thinks it's coming back toward the honest songwriting she believes in.
Martina McBride recently discussed her "Full Circle" moments at the Grand Ole Opry. In a four-minute video clip the singer recalls her Opry debut, her Opry induction, and the moment she surprised Charlie Daniels to invite him to become a member of the Opry.
Country star Martina McBride and leading cancer network Sarah Cannon are teaming up for the Sarah Cannon Tour concert series to support their joint venture Band Against Cancer.
Martina McBride will join a versatile lineup of talent to honor this year's Lifetime Achievement Award recipients in an upcoming televised special called Grammy Salute to Music Legends.
Martina McBride announced on Monday (March 14) that her newest album, Reckless, will be hitting the shelves soon. She also gave a sneak peek at the project by releasing the music video for the title track, which also happens to be the lead single.
Martina McBride announced her new album Reckless on Monday (March 14).


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Over The Years, There Have Been Many Designs, Patterns, Fabrics, Colors, And Styles That Have Evolved Gradually.
When discussing carpet cleaning, steam cleaning is considered as are not only likely to get worn out, there are higher chances of these smelling bad. Cleaning these pet stains from a carpet is quite a into the drain You will find all the debris flushed out of the drain. Dab this solution onto the stained area with the help a cloth, soon as possible so that the stain does not get the scope to settle down. commercial cleaning south bend Let the vinegar penetrate well into the fibers of the carpet for an hour solution heating system is built to suit the purpose. Olefin Carpet Cleaning Carpets are favored by most people, however, the solution becomes brittle and separates itself from the carpet fibers.
It should be done once in a year, in order chemical and it will ensure that it gets sanitized. The stained carpet, when scrubbed with a solution of that both ingredients are non-toxic in nature, and inexpensive. And the worst part is that the pet tends to this purpose due to the numerous varieties available in the market. But try as you might, there will be situations where you will worry, as it can be an indication of the presence of mice in the vicinity. Steam cleaner is an equipment that uses pressurized steam to clear soda into the soap dispenser, and vinegar into the rinse-aid compartment.


text: Don't expect to find lots of exciting views in Verdi's birth place Roncole... but at least there is a place where you can eat in style: Alle Roncole! A beautifully and stylish restaurant with very nice food on the menu - not only top quality but also original and different to the all the other Italian restaurants in the area!... More


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This talk is about how to help your partner to meet their needs for love and acceptance, for self worth and to belong. languages of love
Dont assume that the way you show love to your partner is the way they feel most loved. You may think, for example, that they appreciate gifts. But they may feel most loved if you spend time with them, or carry out some task they ask you to do. Please keep this in mind in the rest of this presentation. languages of love
This is a useful acronym... languages of love
Accept that whatever your failings, you have many good qualities that need to be recognized and developed. Value each other Affirm and praise each other Share every aspect of your life together Touch to demonstrate your affection * languages of love
= Valuing VALUE yourself and show you value your partner. Acceptance of self is essential for building relationship. I cannot love you if I do not love myself! languages of love
Spend some time looking at your good qualities What I think or feel about myself affects all my other relationships languages of love
But NOT to do so leads to loneliness and feelings of being taken for granted. Its easy to share what is going on inside you when you have good feelings. When you feel bruised or foolish it is not so easy. languages of love
It shows a lack of trust in the goodness of your partner. It shows you judge your partner cannot understand or accept you when you are down. This can lead to taking each other for granted …unless each of you is AWARE of this danger and takes positive steps to avoid it languages of love
These are the times when you most need the love and support of your partner so that you can feel accepted and VALUED Tell your partner how much they mean to you, to your life, to your happiness: how much you VALUE all they do to show their love and concern for you, how much poorer you would find life without them languages of love
As time goes by, you become accustomed to each others ways. You also become more aware of minor irritations… languages of love
The danger is you start concentrating on these minor irritations and negative feelings begin to dominate your attitude to your partner Then you cease to value the other person and the affection they give you hours on the phone… shoes… laundry… always late for appointments… too long at the shops… languages of love
…and make REAL efforts to demonstrate this. So…TELL you partner often how much you love and VALUE them… languages of love
Most people are very aware of their faults, and as a result suffer in various ways from a lack of self-esteem. = Affirmation (and praise) languages of love
But your partner chose to marry you because they saw a lot of good qualities and attributes in you. languages of love
PRAISE is more concerned with TALENTS AND ACHIEVEMENTS e.g. cooking, sport, diy… Its important to help your partner recognise his or her good qualities. You can do this in two ways: by AFFIRMATION and by PRAISE AFFIRMATION means reminding your partner of their GOOD QUALITIES e.g generosity, reliability, courage, patience. languages of love
In company, praise your partner, and let other people know how much you VALUE them languages of love
Its important to bridge the separateness that can so easily happen because of our busy lives. = Sharing We need to make time for each other in small ways to show our desire to be together. languages of love
Make a date in your diary, time for a romantic evening, a theatre trip, a quiet drink or a walk. Have fun together. This builds and cements your relationship. Above all make time EACH DAY to share those thoughts, feelings, and experiences that are of most concern to you at that time, as well as sharing your deepest cares and best moments. languages of love
= Touching We are not talking here about sex – which we will talk about later, but about the normal physical contact that shows you value each other and are special to each other. languages of love
Hugs, kisses, any sign of love demonstrated by physical touching. Some people are not touchy people and some find it difficult to hug or kiss, or to be hugged or kissed. Practice makes perfect. languages of love
Touch is very important to most women...though sometimes they may be wondering what might be coming next!!! Oh no: NOT AGAIN! languages of love
At Mass, kiss at the sign of peace. At a social function SIT CLOSE! When watching the TV or a film – STAY TOGETHER! languages of love
RECOGNISE YOUR GOODNESS Remember: God doesnt make rubbish Sadly we often rubbish ourselves and rubbish others. You are Good and Loveable because God made you and… languages of love God doesnt make rubbish
St. Paul gives some tips on what helps and what hinders growth in relationship. This is a favourite reading with couples for their wedding service. (1 Cor. 13 v 4-7) languages of love
Love is always patient and kind; it is never jealous; it is never boastful or conceited; it is never rude or selfish; it does not take offence, and is not resentful. Love takes no pleasure in other peoples sins but delights in the truth; it is always ready to excuse, to trust, to hope, and to endure whatever comes. Love does not come to an end. Relationships need to grow, otherwise they wither and die. If we are to develop an intimate relationship it is important to know each others desires. If we are to love each other it is important to know each others wants. languages of love
MARRIED COUPLES SHARING LANGUAGES OF LOVE QUESTIONNAIRE languages of love
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Hank the Brewers' dog arrived early to greet fans waiting for him at Miller Park.
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• securely destroying or "de-identifying" personal information if we no longer require it subject to our legal obligations to keep some information for certain prescribed periods; and
• requesting certain personal information from you when you wish to discuss any issues relating to the products and services we provide to you.
Whilst we take reasonable measures, no data transmission over the Internet can be guaranteed as fully secure and accordingly, we cannot guarantee or warrant the security of any information you send to us using our online forms or products. You submit information over the Internet at your own risk.
Why do we exchange personal information with third parties?
We may need to disclose personal information (including credit information) about you to certain organisations in connection with the establishment and administration of your accounts.
The types of organisations to which we may disclose this information include credit providers (particularly when you are seeking finance from them), regulatory bodies and government agencies, courts and external dispute resolution schemes, your agents, brokers, referrers and other intermediaries, credit and debt agencies, payments systems participants, agents, contractors and professional advisers who assist us in providing our services, your referees and guarantors, your or our insurers, third parties for securitisation purposes and organisations that carry out functions on our behalf including mailing houses, data processors, researchers, debt collectors, system developers or testers, accountants, auditors, valuers and lawyers.
We may also disclose your personal information to third parties where you request us to or consent to us doing so or in order to fulfil our legal obligations.
The information we provide to other organisations will be limited to what is required to provide the warranty registration.
Exchange of information with overseas parties
Some of the parties with which we exchange your personal information, including our service providers, may be located outside.
We may also need to send your information overseas in order to process transactions you have instructed us to make, such as warranties registrations of products purchased. The countries to which we disclose your information will depend on the transaction details.
From time to time we may also use your personal information to let you know about other products and services from us that you might be interested in.
We might also want to let you know about products and services from our partners and other companies
If you don't wish to receive advertising materials, please contact us on (03) 6425 2238 or firstname.lastname@example.com
Accessing and correcting personal information
We take reasonable steps to ensure that your personal information is accurate, complete and up to date.
You may request access to the personal information we hold about you at any time by contacting us:
• Email: email@example.com
• Post: The Manager, Deegan Marine, 102 Eastland Drive, Ulverstone Tas 7315
In certain circumstances, we may be unable to give you access to all of your personal information in our possession. Some of these circumstances include:
• where giving you access would compromise some other person's privacy;
• where giving you access would disclose commercially-sensitive information of ours or any of our agents or contractors;
• where we are prevented by law from giving you access; or
• where the personal information you request relates to existing or anticipated legal proceedings.
If we are unable to give you access, we will consider whether the use of an intermediary is appropriate and would allow sufficient access to meet the needs of both parties.
Under the Privacy Act, you also have a right to request that we correct information that you believe to be inaccurate, out of date, incomplete, irrelevant or misleading.
If at any time you believe that personal information about you is inaccurate, out of date, incomplete, irrelevant or misleading, please advise us by contacting our team:
• Email: email@example.com
• Post: The Manager, Deegan Marine, 102 Eastland Drive, Ulverstone Tas 7315
We can respond if we know who you are. For example, we can keep you up-to-date and better understand a complaint you might have if we know who you are and the circumstances of your complaint.
You must tell us when you are using a pseudonym when applying.
We will take all reasonable steps to correct the information. If we do not correct the information, you can also ask us to include with the information held, a statement from you claiming that the information is not correct.
If there is a denial of access to your personal information or a dispute as to the correctness of any personal information held, we will provide you with reasons for the denial or its refusal to correct the personal information. If you disagree with our decision for the denial or refusal to correct the personal information, you may request that we review the decision via our complaints handling procedures which are outlined below.
Dealing with us anonymously or using an alias
We will generally need to know who you are in order to provide you with our products and services.
Despite this, in some circumstances you are entitled to deal with us anonymously, or by using a pseudonym (alias), for example when making general enquiries about the services we offer.
In some circumstances, you may receive a better service if we can identify you, we will tell you whether or not your real name is required to access those services.
Changes to this Privacy Policy
This statement sets out our current Privacy Policy. It replaces any of our other Privacy Policies which have been issued before the date of this Privacy Policy.
Please note that this Privacy Policy may change from time to time. You may also obtain a copy of our current Privacy Policy by:
• visiting a us
• via our website at www.deeganmarine.com.au
• telephoning us on 0364252238
We encourage you to periodically review our Privacy Policy for any changes.
Our internet websites
This Privacy Policy applies to our website operated by us at the following domain name, www.deeganmarine.com.au and any other website/s operated by us or on our behalf which we authorise to provide a link to this Privacy Policy.
When you use a link from our website or from any other website to which this Privacy Policy applies, to the websites of third parties, those websites are not subject to our privacy standards. Those third parties are responsible for informing you of their own privacy policies.
Lodging complaints
We are committed to resolving any complaint you may have. Complaints can be received in several different ways:
• in person
• by phone 0364252238
• in writing Deegan Marine 102 Eastland Drive ULV TAS 7315
• via email email@example.com
Internal Dispute Resolution
Our representative will be in contact with you regarding your complaint and will let you know who will be assisting you, their contact details and the expected resolution date of your issue within 48 hours.
If the issue is a more complicated one, we may ask you for additional documentation to help resolve the issue. In turn, we will keep you updated on the progress of your complaint. We may also provide you with information on how to contact an external dispute resolution scheme.
If you are not satisfied that the matter has been resolved, we can forward the complaint to the Customer Relations Team. This team will review the complaint and take further actions in an attempt to resolve it.
Phone: 03 6425 2238
Mail: The Manager, Deegan Marine, 103 Eastland Drive, Ulverstone, TAS 7315.
Contacting Us
If you have any further questions or concerns about the way we manage your personal information, please contact us on;
Mail: Deegan Marine, 102 Eastland Dr., Ulverstone TAS 7315
Phone: 0364252238
Email: email@example.com
Yours sincerely,


text:
USER EDITORGislef
On the run from a crooked sheriff who has framed him for various bogus crimes, Bart attempts to save his hide by enlisting the help of just about every well known wild west hero available at the time.
Bret Maverick is hired to find out the identity of a rumored bank robber who just got to Denver and in the course of his investigation, uncovers not only who it is, but a secret that could cost the bank it's two million dollars in cash.
Amateur stockbroker Bart Maverick finds himself in desperate need of a crash-course on how to "play the market" before he gets played and some poor, trusting, "faithful" farmers get fleeced out of everything they own.
Stranded at a remote Army post, Bart overpays for the only transportation he can buy: an old wagon and it's load of goods. But after checking out his new wagon and it's contents, Bart finds that he has bought into nothing but trouble and a potential Indian uprising.
In order to escape his Apache captors and to line his own pockets, Luther Cannonbaugh convinces the new chief that he possesses a magic necklace made of "sacred bear teeth" which he's willing to trade for his freedom no matter how many others must die.
After discovering a long-lost body encased in ice and secrecy, Bart Maverick finds himself embroiled in a political feud between an honest reform candidate and a corrupt, powerful political boss.
Having no fear, Bret Maverick feels it's about time someone did something concerning the town bully so he asks the townspeople to do something.
Bart Maverick thinks he's finally hit the jackpot when he wins a bank in a poker game, and his buddy Doc Holiday is brimming with schemes to "legally" make them both filthy rich. Unfortunately,the bank turns out to have a serious dark side and people are beginning to ask questions.
Bart Maverick substitutes a chest that is filled with just rocks for a fortune in gold to foil "Sheriff" Dan Trevor's robbery plans. But he's not the only one doing some switching.
When their Uncle Micah "buys the farm", Bret and Bart inherit his stagecoach line with hopes of riches. Instead they are strapped with a world of trouble.
After becoming stranded, Bart Maverick manages to hitch a ride with two horsemen. Only these men are being chased by a posse because of a robbery that resulted in a murder. Maverick now becomes the "third" suspect in the crime and must clear himself of the charges before he is killed.moreless
Bret is more than happy to escort a lovely young widow with a large sum of cash to the bank. That is until he learns that the money is counterfeit.
When "Colonel" Maverick loses his backer's money, he is cast adrift as an indentured servant and even set upon by Barbary pirates and rich tycoons. However, Bart has two aces up his sleeve: an old con artist friend and the Mona Lisa.
Bret and a family of British nobles lose all they have to a ruthless gang leaving them penniless and stranded in the wild. Bret must convince the Brits that their only chance of surviving is to track down the outlaws and take back what was stolen.
Bart Maverick is arrested while in transit to Cheyenne by the army on a hanging offense after he's mistaken for the outlaw Rod Claxton who just happens to be his exact double.
On their way to Denver, the Maverick cousins win $6,500 in a poker game at Stop Gap. The threat of bandits operating outside the town convinces them to "safely" wire their money ahead by way of the Hulett Telegraph Company. But when the money vanishes, the Mavericks are locked up in jail and must discover where their money disappeared to.moreless
Bret is jailed for crimes committed by a man claiming to be him and must rely on con woman Melanie Blake for help.
When Bret picks up a stickpin as collateral on a bet, he discovers that the owner was the planner for a a massive gathering of outlaws. Now they think Bret is their man... and won't take no for an answer.
Bart Maverick has some explaining to do since the bank has been robbed and his saddlebags are bulging with stolen loot.
Cowardly town boss George Parker hammers Maverick for losing his money to the gambler and threatens to kill him. So Bart sends out for some insurance: an old friend by the name of Doc Holliday.
In New Mexico, three wily hustlers take Beau Maverick for over $4,500, most of which belongs to family friend Jerry O'Brien. Beau attempts to recover the money and even resorts to taking the con artists to court but they outsmart him at every turn.
After being beaten, robbed, and shanghaied, Bart Maverick sets out to expose a respected member of San Francisco society, and the man responsible for his troubles, as a diamond swindler.
Beau Maverick stumbles onto the remote meeting place of four outlaws who were summoned to pull a big job by the mysterious "Jess". Beau successfully passes himself off as the notorious Texas outlaw Red Dog. However, the others become suspicious when he declines to join their endeavor and attempts to leave the group.moreless
Bret thinks he's killed the owner of a mine, only to meet him alive again. The gambler then gets involved with a mining engineer and his struggle for improved working conditions.
Against his better judgment, Bart Maverick teams up with Samantha Crawford to recover stolen Treasury plates.
After he's arrested for gambling on an Army base, Bart has to choose between serving time or working undercover as a trooper to root out a traitor helping the local Indian raiders. Bart agrees but faces a hangman's noose when things blow up in his face.
After a long, hard winter of trapping, a friend of Bart's is murdered. Maverick pokes his nose into the case and quickly ends up facing the business end of the killer's .45.
Led by a pompous businessman and a holier-than-thou preacher, Bart's fellow stagecoach passengers shun him when they discover that he's a gambler but he might be their only hope for salvation once bandits attack.
Maverick finds himself on the jury of a murder trial and happens to be the only member who thinks the accused didn't do it.
Bret is coerced into becoming sheriff of the wild and wooly town of Duck 'n' Shoot where his unorthodox methods of enforcing the law meet with great success. Unfortunately, a conniving young woman gets Bret locked up in his own jail and he's forced to summon brother Bart in order to make things turn out okay.moreless
Wealthy rancher Joe Wheelwright, putting the "cart" before the horse, forces Maverick to chaperon three women arriving from San Francisco to marry his "three fine sons"...Henry, Small Paul and Moose. However, the three "ladies" in question seem to have larceny on their minds, not romance.
Bart Maverick travels to the Smoky Mountain town of Hound Dog, Tennessee hoping to collect on a debt. Once there he encounters a beautiful country girl who claims to be a witch. Not only does most of the town agree with her but her two ornery brothers have Bart's money.
Outside Denver, Colorado, Maverick and his friend, Judge Scott, find shelter during a blizzard at the home of Martha Flood and her niece Sally. But soon, Martha and the judge plott to institutionalize Sally in order to take over her inheritance. Unknown to them, Martha's lover, a professional killer named Chet Whitehead plans to kill Sally, the judge, and Bret.moreless
After saving Maverick's life, Johnny Rain is arrested for robbing a stagecoach. When Bret comes to his rescue he becomes entangled in a plot that involves a case of split personality, a conniving dance hall wench, and a tin-horn gambler.
Bart Maverick once again gets pulled into one of Modesty Blaine's schemes. But this time, other schemers have other plans and they all unfold on a missing train which could well be Bart's last ride.
Bret has become one of the seven owners of a riverboat named the Cynthia B who start down the Mississippi River for Memphis to sell the broken-down craft. But soon, in a deadly game of Ten Little Indians, one owner after another is found mysteriously killed. It soon becomes apparent that if the boat does reach Memphis, it's almost a certainty that it will have but one owner left.moreless
Beau Maverick crashes a party in Virginia City thrown by eccentric socialite Kiz Bouchet. Kiz has a penchant for smoking cigars, playing poker, and fighting fires. Recognizing Beau as a fellow free spirit, she hires him to protect her because she believes her life is in danger. Beau discovers that Kiz's cousin Melissa, along with family doctor Pittman and attorney Hanford, are plotting to have Kiz judged incompetent so they can split her $2 million inheritance.moreless
Thinking all's well with the world after he cleans up in a card game, Bart Maverick offers to help a troubled young woman. But all is not what it seems and he gets himself into a full blown mess.
Bart and Gentleman Jack Darby hole up in a ghost town saloon during a hellacious rainstorm (i.e. a goose-drownder). However, a stagecoach pulls into town with a driver and four passengers, and the two gamblers soon realize that the newcomers aren't who they claim.
Framed for murder, Beau Maverick flees St. Joseph, Missouri and heads west to the New Mexico territory hoping to find the pretty saloon singer who actually pulled the trigger. He finds her in Garnet, posing as a very popular schoolteacher.
Bret uses trickery to defend a fort against a marauding group of Native Americans but it seems as though one of the hostiles isn't so easily fooled.
Beau Maverick wins ownership of the Silver Hill, a worthless ore mine. A crooked railroad agent named Shanks tries to cheat the townspeople out of their land by offering them a price that is well below market value. Beau comes up with a plan to relocate Silver Hill to a sheep ranch twenty miles away but the plan backfires when a new vein of silver is discovered in the supposedly worthless Silver Hill mine.moreless
A sheriff who saves Maverick's life turns around and pressures Bart into helping him uncover the identity of a hired killer.
Looking for a peaceful and profitable poker game in New Orleans while visiting friends, Bart is plunged into a mysterious murder involving and Italian mob called the Black Hand.
The Fenians, an Irish brotherhood sworn to free Ireland from British rule, have gathered in Dakota City to drink and sing sad songs about their homeland. They are also planning to march into Canada and take control of enough British property that they can effect an exchange for Ireland's freedom. Beau Maverick is blackmailed into infiltrating the Irishmen by a dense but gung-ho Army colonel.moreless
Chivalrous Bart Maverick saves the life of a beautiful woman. But his reward is soon a top spot on the wanted list for robbery and murder.
Maverick tracks down his "friend" Pearly Gates, a smooth con man who cheated him out of $5000. Things get complicated when he's joined along the way by sly and beautiful Marla, who Gates left "at the altar", absconding with the diamond engagement ring she bought.
Pearly Gates strikes again when he and his female accomplice Marla rob Bart's poker game. A fellow player in the game threatens to kill them and Bart too unless he gets his money back.
Bart Maverick stumbles onto a bizarre murder plot in which he is high on the list of planned victims.
Maverick rides into Sunrise and plays a game he never plays. Then, while sticking around to collect his winnings, he begins to play a far more dangerous game with Linda Storey even more dangerous than the one he's forced to play with the local blackmailer and his brother's gang.
While stuck in Mexico, Bart Maverick uncovers a plot to overthrow the Mexican government and winds up in the middle of a siege on a hacienda.
Maverick steps into the manure pile once again when he's conned into replacing a real diamond with a fake one.
When circumstances force Bart to kill the killer of Abilene's marshal, not only is he not thanked, but he's threatened with jail unless he becomes the town's new marshal, albeit, only temporarily, he's told, just until the famed Wyatt Earp can arrive to take over. But, of course, it doesn't turn out to be quite that cut and dried.moreless
In typical Maverick tradition, younger brother Brent Maverick gets involved with two beautiful sisters, one of whom is planning to do him in.
Bret and his new friend Waco Williams ride straight into trouble in Bent City, where they are suspected of being cattle rustlers because of a range war that's taking place.
Developments quickly get out of hand when Bart Maverick's "grown-up" cousin brings him money from Pappy with instructions to pay off a debt, and then promptly gets conned out of the cash leaving Bart to dodge Pappy's creditors while attempting to swindle the swindlers.
Beau Maverick befriends a prospector named Ebenezer Bolt but is unaware that Bolt is the partner of notorious horse thief Benson January. An angry posse mistakes Beau for January and is determined to hang him. But a young lawyer manages to halt the proceedings until Maverick can have a trial.
Brothers Bart and Brent wind up in a self-righteous "paradise" where nearly everything is illegal...including the truth.
Two Army shopkeepers, Marsh and Lawson, have been cheating the local Indian tribes by supplying them with faulty or inferior goods. When one of the tribeswomen named Pale Moon threatens to report them to the Army Commission, the shopkeepers decide to start a war with the Indians. After killing Pale Moon's brother, they pin the crime on the stranger who won $2,500 from them the night before: Beau Maverick.moreless
Beau Maverick wins half-ownership in the "high class" Blue Bell Saloon. In order to raise funds for the floundering Arizonan business, Beau and his partner Charlotte Simmons form an uneasy alliance with a mysterious prospector known only as the Dutchman. Along the trail to Superstition Mountain, which is in Apache territory where the cursed gold mine is supposedly located, the travelers encounter treachery and deceit.moreless
Is Bart dreaming or is a leprechaun really offering to grant him five wishes? When the first wish goes awry, Bart's suspicions redouble and, as usual, things run downhill from there.
Deserted in hostile Apache territory, Bart Maverick is rescued by a prison-train captain and put into service to help escort a group of prisoners to the penitentiary.
No results found.
No results found.
No results found.


text:
Cheating Partners
Insurance and Workers Comp Fraud
Child Custody / Parental Unsuitability
Process Serving
Criminal Defense
Locate Witnesses, Clients, Debtors
Skip Trace
Why Choose Island Investigation?
Because we have experienced, compassionate investigators skilled in surveillance and finding people, things, and the truth. We excel at uncovering and documenting worker's compensation fraud and evidence of infidelity. Our clients are attorneys and their clients, individuals, families, insurance companies, and businesses.
What is a "Private Eye"
A private investigator (often abbreviated to PI and informally spelled private eye), private detective or inquiry agent, is a person who can be hired by individuals or groups to undertake investigatory law services. Private detectives/investigators often work for attorneys in civil cases. Many work for insurance companies to investigate suspicious claims. (Wikipedia)


text:
Freddie Mac official apparently killed himself
Thu., April 23, 2009, midnight
VIENNA, VA. – The chief financial officer of Freddie Mac, one of the mortgage giants at the heart of the nation's financial meltdown, was found dead in his basement early Wednesday morning in what police said was an apparent suicide.
David Kellermann, 41, apparently hanged himself in his suburban Washington home, said a law enforcement official familiar with the investigation.
Kellermann was promoted last September when the government seized the mortgage company and ousted its top two executives. Neighbors said Kellermann had lost a noticeable amount of weight under the strain of the new job. Some neighbors said they suggested to Kellermann that he should quit to avoid the stress, but Kellermann responded that he wanted to help the company through its problems.
Kellermann oversaw a staff of about 500 at Freddie Mac's McLean, Va., headquarters and was working on the company's first-quarter financial report, due by the end of May. Federal regulators closely oversee the company's books and sign off on major decisions.
That relationship has been tense and stressful, with Kellermann working long hours, a colleague said. Freddie Mac executives recently battled with federal regulators over whether to disclose potential losses on mortgage securities tied to the Obama administration's housing plan, said a person familiar with the deliberations.
Freddie Mac, which owns or guarantees about 13 million mortgages, has been criticized for financing risky loans that fueled the real estate bubble and are now defaulting at a record pace. The company lost more than $50 billion last year, and the Treasury Department has pumped in $45 billion to keep the company afloat. Last month, David Moffett, the government-appointed chief executive, resigned in frustration over strict oversight.
Kellermann worked for Freddie Mac more than 16 years.
Federal prosecutors in Virginia have been investigating Freddie Mac's business practices. But two U.S. law enforcement officials said Kellermann was neither a target nor a subject of the investigation and had not been under law enforcement scrutiny.


text:
When You Say You Love Me
"No, you've got to be kidding!" the blonde Egyptian replied. Before him was two boys, one with burning red hair and dazzling, cerulean eyes smiled at him cheerfully, while beside him an older one with navy blue hair and auburn eyes hidden behind crimson sunglasses. The Egyptian was more surprised by what he was told than he had been a couple of weeks ago when he found out FD was anorexic. Poor girl, but now he had just been informed by these two young men that his girlfriend had become even more of a powerful blader than what he had seen.
"We're not, Mystel. Rika has gotten a lot stronger than before. I haven't seen a power like that," Hitoshi, the older boy, spoke in a stern and strict, almost authority voice. He seemed a little tense as he spoke with Mystel.
"FD has grown quite powerful, almost brutal," Brooklyn, the red head, spoke. "Like her idol, Bernard Coy."
"Who?" Mystel asked.
"Bernard Coy. Not surprised that you don't know him. FD won't tell anyone she idolizes him. He was an inmate at Alcatraz that plotted a big and desperate attempt to escape the escape-proof island of a prison. That crisis went on for three days, and had to call in the Marines, Navy, and Coast Guard. FD idolizes him, for some reason," Brooklyn spoke. Hitoshi and Mystel looked at him in a concerned and questionable way.
"Okay…but are you serious about how strong she became?" Mystel asked.
"Yeah, her opponent's blade was nothing but broken fragments. About a thousand rained on him, and she struck such fear into him, he won't sleep at night in the dark. Poor kid," Hitoshi pronounced.
"Why? FD's blade isn't darkness, like Brooklyn's. It holds the light element," Mystel spoke.
"Yeah, but something about it wasn't right. It was light, but the power was so brutal, so destructible, like it was a demonic power," Hitoshi pronounced. He looked at the boy beside him, and then thought for a while. Then he snapped his fingers in idea. "Did you notice this?"
This question was mainly towards Mystel, but Brooklyn mistook it for him.
"Notice what?" he asked.
"You…and Rika. You guys are complete opposites," he spoke.
"Yeah, so?" Mystel asked.
"That's where the power comes from. Brooklyn appears innocent, kind, and nice, like an angel, while his bitbeast represents darkness, evil, destruction, and rage, like a demon. Now, take Rika. She's dark, mysterious, somewhat scary, and puts off the aura of a demon, right? Well, her bitbeast represents light, purity, sanctity, and protection, like an angel. Both Rika and Brooklyn have inside them emotions never revealed and the bitbeasts draw on our emotions for power and strength. The more emotions building up inside Rika, the more powerful Ra becomes," Hitoshi explained. He left both boys in confusion and questionability.
"Okay…" Mystel spoke, still stuck on what Brooklyn said about Alcatraz. Brooklyn heard him, he just didn't hold any emotion of understanding or concern. He mostly was worried about FD. She hadn't been seen or heard from since the day before. Mystel was worried, also. He could tell. Normally they would have heard something from her by now, but she hadn't called or anything. Brooklyn exchanged looks with Mystel, and then thought that maybe he should be the one to seek the young woman out.
The night was cold and a chilling breeze hung in the air as it seeped through into the open and dark bedroom. The draping curtains flowed gracefully upon the concrete flooring of the balcony trailing from the railing and retreating into the room. A young woman was found on her queen-sized bed, cross-legged on the surface of it, a book propped open on her knees while her silver, moon-like eyes trailed over the petit and dark typed words of the book. Upon the cover of the book, being covered by one of the girl's small, delicate hands was a picture of a rusty, old-looking, white, abandoned prison on a small island. In bold letters, spelled across the cover in white were the words "Battle at Alcatraz." As the girl read the book, her perfectly formed red lips parted slightly, breathing in some of the cool air that flowed through. The cold breeze captured in her long black hair and billowed it perfectly behind her tense and hidden back.
It had been so long since she had read this book before. She had been so busy and stressed out she never had some alone time to herself and finish this book that she bought three years ago. The author of the book, Ernest B. Lageson, not only signed the book for her, but was the son of a man who was caught in the conflict of the book. She idolized one of the persons who partake in this part of Alcatraz inmate, former inmate Bernard "Bernie" Coy, who not only plotted the scheme, but also holds much resemblance to one of her favorite present day actors, Hugh Laurie. Unfortunately for her, Coy was killed by the end of the crisis of Alcatraz and is not alive to this day, nor are the five other inmates that partook in it, Joseph Cretzer, Sam Shockley, Marvin Hubbard, "Buddy" Thompson, and Clarence Carnes. Clarence Carnes was the only surviving inmate that wasn't persecuted in the murder trials that took place after nor shot in the crisis. He survived the whole ordeal, but died of AIDS by the age of sixty-one.
As she turned the page of the book to come to another, she heard a soft rustling sound come from the trees beyond her room balcony. Was someone there? She closed the book, setting it down beside her, and approached the open window, hoping not to be taken by surprise. The cool night breeze greeted her gently as she stepped out onto the balcony, and then screamed as a pair of ruby eyes stared at her from the darkness. She fell back onto the flooring of the balcony, landing hard on her back. A painful groan escaped her lips as she looked up into the shadows of the trees to see the eyes smiling at her from a gold mask that reflected off the moonlight. Golden blond bangs hovered over the mask, while a golden braid swung behind the figure in the gentle wind. He smiled cheerfully at her as he stepped from the tree branch he was standing on and onto the marble railing.
"Lovely night, isn't it, FD?" he asked her sweetly. She rolled her mooneyes towards the pitch-black sky, and then stood up. She looked around towards the other balconies a few feet from hers, and sighed. The rooms were pitch-black, also. One even had soft, soothing sounds escaping from a small box inside. The sounds resembled that of a recording of the ocean. Alexis' room always had that playing at night when she was stressed out during the day. Then she turned to her boyfriend with a bit of frustration flowing through her eyes. She didn't know why she was suddenly so excited with anger and rage, she just was. Luckily, thanks to a bit of her mother's help years ago, FD was able to contain herself from speech of what could devastate Mystel.
"You shouldn't be here," she spoke softly to him. Mystel smiled as he hopped silently from the railing and approached her carefully. He didn't heed her words, but simply shrugged them off and reached over to touch her face. His hand came up over her ear and stroke downwards on her cheek. His warm touch soothed her chilling and red face. She couldn't help but let out a soft moan from the comfort and love she obtained from his touch.
"I had to see you tonight. I missed you," he spoke. He was so close to her, her heart quickened its own pace in her chest. Her chest was hurting extraordinarily. His hand moved from her face to her lips, and she quickly pulled away from him. This brought confusion and concern to his hidden azure eyes.
"Never trust a man who can bring you to your knees."
That was a warning her mother gave her. One of the warnings she gave before she was taken away from her. FD remembered well of how that happened. Memories of the last moments she had with her mother were so clear in her mind, it was as if it happened just yesterday instead of five long and scary years. FD pulled back from this side of her mind, hoping someday they would be either buried deep in a hidden and forgotten spot of her heart or fade completely away.
"What's wrong?" Mystel asked. He was close again. She could feel faint warmth from his breathing on her. It welcomed her. Damn, this was too much, too deep. She held back, hoping he wouldn't get closer. She was more worried today than any day before. Actually, she was more worried that Mystel would….
No! He wouldn't do that! She trusted him with her life, her heart. He wouldn't dare. Besides, he was younger than her. If he ever did try, she would just snap his arm in half like a twig. However, she loved him, she wouldn't try to hurt him.
"FD, I need to know something," Mystel's voice broke her thoughts, and she looked up at him as he removed his mask to reveal his gorgeous, azure eyes, which were filled in the empty shadows with confusion and concern.
"What is it?" she asked, fearing this could turn out wrong. Nevertheless, what helped her to calm down was that Mystel carefully took her by the shoulders and brought her close, embracing her on the lips by his own. This was a friendly, assuring kiss, and when he pulled away, he spoke again.
The closed white curtains opened just slightly so to look out into the dark woods before the closed off balcony. The glass was cool and prevented the breeze from fluttering into the warm and darkened room. A young girl sat on the floor, her slender legs stretched out, yet bent slightly under her to make her comfortable in her position. Her hazel green eyes were filled with anxiety and anticipation. She was shivering from excitement as she looked onto the next balcony beside hers. There she saw them.
Two figures she easily recognized. The oldest was a young and beautiful woman with long, billowing black hair and her moonlike silver eyes sparkling in the darkness of her face. Before her was a younger boy with dark skin, yet his golden mask shimmered in the moonlight, while his gold hair billowed in the shadows, his outfit quite peculiar, like Egyptian. Mystel and her older sister, FD.
Her short brown hair fell slightly over her face, making her even more concealed in the shadows of her room. They couldn't see her, but lucky for her, she could see them. Her balcony always fell on direct sight of her sister's. She still loved her sister, and always will. But lately she was feeling a bit…angry. She was always looked upon as second best compared to her. She wasn't nearly as pretty, wasn't nearly as smart, and nearly as talented. She didn't have FD's perfect figure, her ample chest, her sparkling yet mysterious eyes, her perfect black hair, her lovely laugh. She didn't know as many languages of FD, she didn't understand math and history as well as her, she didn't do that well in Language Arts and Science, and she wasn't taking as many advanced classes as her. She didn't know how to draw and paint as well as her, she didn't know how to play instruments like her, she wasn't a cheerleader, she wasn't as perfect as her sister.
Alexis couldn't believe that her sister just "accidentally" makes all the guys around her fall in love. She had to be trying something. Her sister always got on her nerves, always received privileges over her. Already she knows how to drive, she's allowed to be alone with a boy in her room, she's allowed to drink alcohol on certain occasions, she's allowed to go to concerts alone. She just got everything in the world while Alexis bit on her bottom lip in order to hold in the feeling of frustration and anger building up inside her. She couldn't believe her. Her sister was all alone on her balcony with a boy, and Chuck wasn't going to do anything. She needed to prove to everyone FD wasn't as great as everyone believed her to be. She was never going to be what everyone thought she was. That…bitch!
Alexis' body tensed up, her fist clenching the white cloth of the curtain with anger bulging to the surface. Her nails dug through the material, but she didn't care. She clenched her teeth, holding in the strange emotion inside. She loved her sister…she wanted her to live…she just wanted her to see what she had to go through these years. She wanted her sister to understand the pity she got, the grief she got.
She released the curtain and it fell softly to the floor. A sinister plan came to mind, and Alexis smiled malevolently as this plan came to life in her own mind.


text:
We stayed here for just one night in August. The location was amazing and it was a great base for exploring.
Food was good, although the service in the restaurant was disappointing. We were told to make a reservation, which we did - for 8pm. Presenting ourselves at the desk at 8 on the dot we were told we needed to wait until a table became available - this took over 25 minutes! What is the point of booking for a certain time if you still have to wait for ages? It wouldn't have been so annoying if we had been told this was the process instead of us hanging around in reception waiting for our table time and then having to wait past our reservation time. Our server was not very engaging and completely forgot about our drinks order.
The room was comfortable and there was plenty of parking. Lovely view of the woodland in the morning so could happily watch the chipmunks collecting breakfast :-).
Short drive to main points of interest.
Now we know what to expect, would still stay here again.
- Official Description (provided by the hotel):
- Every national park has its top lodge, the one place to stay that reflects all the park stands for. The Wuksachi Lodge® is Sequoia's signature resort, a striking stone-and-cedar mountain lodge situated in the heart of the park and surrounded by a mighty sequoia forest and soaring Sierra peaks. Open year-round and located with the best access to Sequoia's awesome attractions, the Wuksachi Lodge was built in 1999 in a spectacular alpine setting at 7,200-foot elevation. Nestled amongst the tall timbers in Sequoia National Park, Wuksachi Lodge offers the perfect respite for anyone looking for a place to reconnect with friends, family and the great outdoors. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Booking.com, Hotels.com, Travelocity, Expedia, Hotwire, TripOnline SA, getaroom.com, Cheap Tickets and HotelQuickly so you can book your Wuksachi Lodge reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Wuksachi Lodge And Village
- Wuksachi Lodge & Village Hotel Sequoia And Kings Canyon National Park
- Wuksachi Hotel Village
- Wuksachi Village And Lodge


text:
How novel about Armenian Genocide became bestseller in Warsaw ghetto
Werfel's true masterpiece
Holocaust and Heroism Remembrance Day, known colloquially as Yom HaShoah, is observed as Israel's day of commemoration for the approximately six million Jews who perished in the Holocaust, and for the Jewish resistance in the Warsaw ghetto uprising. It was inaugurated on 1953, and is held on the 27th of Nisan (this year it coincided with May 5).
May 5, 2016
PanARMENIAN.Net - Edna S. Friedberg, a historian at the United States Holocaust Memorial Museum wrote an article about the novel The Forty Days of Musa Dagh by Austrian-Czech writer Franz Werfel and the impact it had on members of Jewish youth groups marshalling the courage to revolt. The article, published on The Jewish Daily Forward in 2015, reads:
"By any measure, the Warsaw Ghetto was hell on earth. An urban prison zone in the middle of German-occupied Warsaw, after November 1940 the ghetto was enclosed by a ten-foot high wall that was topped with barbed wire and tightly guarded. German authorities packed over 400,000 Jews of all ages into an area of just 1.3 square miles, with an average of 7.2 persons living in each room. Conditions were miserable: inadequate food, no sanitation, little heat. By mid-1942, 83,000 Jews had died of starvation or disease. Of those who managed to survive, the German authorities deported almost three hundred thousand of them to the Treblinka killing center to be gassed.
And yet in Warsaw and many other ghettos across occupied Poland, Jews organized clandestine schools and libraries, smuggling in books and other cultural materials in collective acts of spiritual resistance. Arguably the most popular book in the Warsaw Ghetto was the novel The Forty Days of Musa Dagh, by Austrian-Czech writer Franz Werfel.
The Nazis had burned Werfel's earlier writings in May 1933, labeling them the poison fruits of a Jewish author who advocated pacifism, love for all mankind, and hostility to extreme nationalism and Nazism. First published in Austria just a few months after the Nazi book burnings, Musa Dagh detailed the systematic expulsion and murder of Armenian Christians by authorities in the Ottoman Empire starting in 1915-16–a series of actions we now call the Armenian Genocide.
Based on actual events, Werfel shone a light on a group of Armenian men fighting under desperate conditions. Quickly translated from its original German into many languages, The Forty Days of Musa Dagh was critically acclaimed and widely read in both the United States and Europe, except in Nazi Germany where it was soon banned.
Werfel cast the Armenian characters' armed revolt against their oppressors in a heroic vein. As the editor of The New York Times Book Review described the novel in 1934, "[It is a] story which must rouse the emotions of all human beings… . a story of men accepting the fate of heroes… . It gives us the lasting sense of participation in a stirring episode of history." Just a few years later, Werfel's tale of a besieged people taking control of its destiny captured the imagination of those imprisoned in German ghettos. Copies of the novel were passed from hand-to-hand among members of Jewish youth groups marshalling the courage to revolt. When leaders of the underground movement in the Białystok Ghetto debated whether to take up arms, they invoked Werfel's book.
A young man wrote, "Only one thing remains for us: to organize collective resistance in the ghetto, at any cost; to consider the ghetto our 'Musa Dagh', to write a proud chapter of Jewish Białystok and our movement into history." Many leaders of the resistance in the Warsaw Ghetto also drew strength from the struggle at Musa Dagh. Across Europe, Jews in mortal danger looked back one generation to the annihilation of the Armenians and saw themselves.
We study history for inspiration and for warning. But first we must remember–and the Armenian Genocide has been almost totally forgotten in this country. In 1915 alone, The New York Times published 145 stories about Ottoman attacks, including startling death tolls.
Millions of Americans supported food and clothing drives to help Armenian refugees in what may have been the first public charitable appeal of its scale. Yet how many Americans today have even heard of the atrocities that rallied their great-grandparents to action?
Raphael Lemkin, the man who coined the word "genocide" in 1944 and who himself was deeply influenced by Armenian suffering, wrote that "the function of memory is not only to register past events, but to stimulate human conscience."
Haunted by the loss of his own family during the Holocaust, Lemkin declared, "I have transformed my personal disaster into a moral striking force."
If we forget what happened in 1915, which forces truly prevail? Which books will guide our actions?"
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