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|Topic Review (Newest First)
|Excellent reference Shaggy. This is top-notch information.
Please dont lump Pro-Topline into the mess because I have them and I know many others who are very happy with them. All the issues people had with topline were issues i had seen in many manufacture of heads but people felt the need to bash them. Now that they are run by RHS you shouldnt have a problem getting a quality product. Back on subject this is how I found out about the pro-comp. I was pretty shocked that they would cheap out on some serious materials they use on their products. Just a matter of time that hotrodders rides burn up in flames.
|Unknown Poster
|To experience either Peter or his son stand there and tell you their product is just the best, and hear how much profit you are going to glean off the sales as one of their "select" dealers.....is a real experience you really don't want to have. A real, first rate con job.
|sounds like the Walmart mentality
|Unknown Poster
It's no small coincidence that Pro-Comp is named that. Peter, a Czeckoslovakian whom livesd in Australia, whom also owns Sydney Speed and Marine, in Sydney, also owns Pro-Comp. I understand from a few Austrailian and new Zealander people I know, Sydney Speed and Peter are to be avoided, so same with Pro-Comp.
His main reasoning with naming his knockoff businesses Pro-Comp, was with product name association. Pro- is used in both Pro-Comp...and...Pro-Form. If yoiu were looking for an item made by a company you only knew started with the word "Pro", you'd have a 50/50 cjance to hit either one. So...this gave Peter a 50 percent chance of getting people looking for much better Pro-form products to his less than good parts.
I was approaced by him, personally, to handle and use his parts, and I declined. I had heard of many unhappy experiences/dealings with both him personally, and his parts, from other hot rod builders I know in the industry. I had also seen some of this parts, and came away with less than a confident feeling about them.
I understand Peter will make a dealer out of just about anyone, just ask him and, bango, you are an instant dealer. So much for protected sellers. I think this is the reason we see so many Pro-Comp new products for sale on E-bay, just too many sellers who are getting the message, the parts are not what they are advertized to be, and anybody and everybody are dealers.
From what I am told, with Peter, it is all about two facts, putting other manufactureres out of business if possible, by flooding the market with low cost, poor quality knockoffs, and the bottom line profit margin.
I was told, by the brodix dealer that the 'IK' heads cannot be ported out, they are the way they are, no room for opening them up. Thats why they are as cheap as they are. The 'RR's' have more meat, cost a bit more, but can be opened up with porting.
Thanks Larry3 for the info on the Brodix being totally made here in the good ol US of A.
Its bad enough that there taking our jobs from us but I'll be damned if I'll buy that junk and put it on my chevy. Everyone who uses that communist China crap will surely pay dearly in the end plus you'll have to go get an American made part to fix it.
Aluminum Heads from USA
Hey Guys,
Appreciate all the response and feedback to my little tirade!
I do know about Brodix...I have been in their building, they do their own casting (its a foundry) and machine work.They also have at least one Dyno (1993). My mistake was not looking at their street/strip heads, and the prices of the racing heads $1500--2000+ was more than I was willing to pay. The IK180 & IK200 series are $1000--1400 depending on options chosen. If I had spent that 2-300 more, I would already be through with all my head work!
Anyway, thanks again for all the feedback and Info.
What timing on this topic! Sorry to hear about your situation Larry3. I did a ton of checking looking for a steal of a price on a set of al heads. Saw those super flows from the same site, but they were supposed to come from Australia which raised some flags. I remembered some cheap imitaion aluminum parts coming from there a couple of years bak, so decided to steer clear. Glad I did now.
Ever notice how many of these 'new' cheap priced al heads have 'Pro' attached to their name? Makes a guy wonder when you then hear some of the names associatedwith the name, and ending up with a crappy result after tons of additional work to make them work right. I know just because they have 'Pro' attached to the name really doesn't tie them together, but when most seem like junk?, hmmmm.
I ended up buying a set of Brodix RR200's and they should be here next week. Bought them from tonybarkerracingengines.com out of Mendenhall, Ms. Got a great price on them, some Corteco head gaskets and Felpro intake gaskets. Hopefully they are 'bolt on ready' like they are supposed to be.
One thing I was told by a few different people and I cannot verify it, so take it with a grain of salt. Most al head castings are made in China with the name brands being machined and built here. I hope someone can prove that wrong because, like someone said earlier, we need to keep our manufacturing wizardry here and not overseas.
Please, prove this wrong, someone.........
|machine shop tom
I checked out one of the heads I have here. The Engine Pro part# is PFS191202-2A. The part# is a tag that is suprimposed over another part# tag that reads (in part)C3002S>
The heads don't look bad. The valve job itself is among the best I have seen on aftermarket heads. The head I checked is warped .012", not unlike other aftermarket heads I have seen. The spark plug holes are washer-seat, with a 7/16" reach. They are unported, need bowl and port matching and clean-up.
That's all I have on them.
|Unknown Poster
Look very closely at the valve seat areas, I have seen 4 heads sold by a friend that is a machinist, drop the seats right out of them, both intake and exhaust. No, the heads weren't ported or modified, they were right out of the box new and all 4 heads went less than a thousand miles before disaster hit.
And, the other areas mentioned in this topic as well.
Pro-Comp seems to be a company just bent on knocking off other's parts, for profit only. That is just what they appear to be. I agree, the bitterness of poor quality long remains after the sweetness of cheap price is forgotten. The main issue(s) with Pro-Comp seem to be that they exude that their parts are the greatest thing since holes in donuts, when they clearly aren't.
|machine shop tom
I am following this thread with interest because I have a set of Engine-Pro aluminum heads in my shop right now. I believe they are the same as the ones that Larry3 is talking about. I'll check them out and let you guys know what I find (when I get a little time )
No need to worry about the CHI-COMS. They need our money to pay Americans for stolen military secrets. Either the Arabs or illegal Hispanics will get to us first across open borders...
|Thanks Larry for sharing your expierence. I really hope that you get everything squared away. I have bought a few "knock offs" and luckily I have had good luck, but I am sure my day in comming. Appreciate you informing us, that is why this site is so important.
|Unknown Poster
|I am very glad to see that others are finding the same negative things out about Pro-Comp as I already have.
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Charleston, SC, USA < change city >
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Tides Folly Beach
1 Center St.
Folly Beach, SC 29439, USA
Reception Sites, Hotels/Acc...
Recommended in 28 weddings
1 description
Beach At Isle Of Palms
1202 Palm Boulevard
Isle of Palms, SC, United States
Beaches, Hotels/Accommodati...
Recommended in 9 weddings
3 descriptions
This beach area is right at the area called "Front Beach" - where there are souve...
Folly Beach
Folly Beach, SC
Folly Beach, South Carolina, US
Attractions/Entertainment, ...
Recommended in 8 weddings
3 descriptions
Folly Beach, Charleston, SC. The surf's up at Folly Beach. Six-mile long Folly Be...
Isle Of Palms Beach
1120 Ocean Boulevard
Isle of Palms, SC, United States
Beaches, Attractions/Entert...
Recommended in 5 weddings
4 descriptions
The 'other' beach in Charleston. Isle of Palms or IOP is less popular with colleg...
Folly Beach
Charleston County, SC, US
Recommended in 2 weddings
2 descriptions
A beach. Enough said. Plan to get here early, as traffic increases throughout ...
Sullivans Island Beaches
Atlantic Ave
Sullivan's Island, SC 29482
Beaches, Attractions/Entert...
Recommended in 2 weddings
2 descriptions
The "locals" beach. Important things to know: get there early (before 9) or late ...
Folly Beach
Beaches, Attractions/Entert...
Recommended in 2 weddings
2 descriptions
This beach has more of a grunge feel to it. It's very popular with college studen...
Folly Beach
Ceremony Sites, Beaches
Recommended in 2 weddings
0 descriptions
Folly Beach
Folly Beach, SC
Recommended in 1 wedding
1 description
Known as "the edge of America," Folly Beach is a seven-mile-long barrier reef loc...
Sullivans Island
Sullivan's Island, SC
Recommended in 1 wedding
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For the record, this is Laura's favorite beach; and here is why: casual, unhurrie...

subset: commoncrawl


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I see ghost flowers...
The petals are so delicate, just beautiful.
skatimmy737 wrote:I see ghost flowers...
At first glance, I thought they were jelly fish. but this is a good shirt to bring in this year's early spring
Are they reaching for sun or rain? Or a rag to clean off the window you're looking through. Conveys a haunting sense of fragility/delicacy though.
Lovely design. Congrats. *Ach-oo!!!*
Ah, I love springtime when the haunted flowers are in bloom...
Blue into the bloom. Bloom in the blue.
Condition: Similar to an onion.
So the shirt's an ogre?
Gorgeous. Absolutely stunning. That has to be made into a poster.
tbwulf wrote:Condition: Similar to an onion.
So the shirt's an ogre?
A parody newspaper.
Interestingly, the blur/out-of-focus effect on this shirt is similar to the style of the music videos directed by Samuel Bayer, who did many of the videos from the In Bloom era, including Smells Like Teen Spirit (but not In Bloom itself, unfortunately).
The flowers almost look as if they are a night blooming variety. Very cool.
So if this is how bees see the world with their red-blind vision, how would real bees see this shirt? More importantly, would they try to pollinate my shirt?
I sneezed just looking at it. Blahh
This shirt tells the world: "Show me your stamen."
Haha! Gross.
hey. mi is black! i wooted!!
If there's one thing I don't need, it's another shirt. But what would be the point of being alive without having this shirt? Buy I did.
Brings back memories of Avatar!
Nirvana reference?
+9001 points for Woot
I wish it was printed across the entire shirt.
The best description for a shirt on woot.
AWW, I thought it was jellyfish. =(
I said it before ~ I'll say it again...this is beautiful. First shirt I paid to have overnight delivery ~ can't wait.
It almost looks like a photograph.
It's a beautiful shirt. Unfortunately, my first Anvil women's shirt arrived the other day, and That will be my last shirt.woot. It somehow manages to be both snug in the chest and enormous around my waist and hips.
That's lovely!
I love the write up.
It's so dark, I'm concerned the ink won't contrast very much... but it's a very pretty design.
So what flowers are these? At first I thought maybe California poppies, which are orange during the daytime, but I think there are perhaps too many leaves.
This write-up is full of win.
Photoshop blur filter?
Depth of field. Literally!
Congrats on a handsome shirt, kdeuce.
Beautiful design! Is the shirt for men in AA?
I want this shirt sooo bad but the new women's shirts are so scratchy and irritating in the armpits that I can't wear them. I tried, I really did.
Does anyone know if the kid's sizes or the men's shirts are any better quality, as far as softness goes?
Seriously woot, I want to throw money in your pockets but I just can't with the low quality blanks you're now using. I voted for this one hoping you'd still have some of the old AA blanks for this design!
Beautiful shirt. Woot please bring back good quality comfortable shirts. We love the designs and want to give you our money. Heck, raise the cost another dollar if that is what it takes to get shirts that we can wear in comfort. It does no good to buy a shirt if we won't wear it. Thanks.
If I want some flowers on my shirt, I'll make some biscuts.
Love the ephemeral look of the flowers, wonderful job! And awesome write-up, Woot!
looks like Lord of The Rings.
A Elbereth Gilthoniel!
View All →


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The Mojo Makers have some beautiful inpsiration for you this week. Check out their takes on this week's challenge below...
|Julee Tilman
Poetic Artistry
|Maria Gurnsey
Scraps by M
|Mary Fish
Stampin' Pretty
|Teresa Kline
Enjoy the VA Sunshine
|Wendy Bond
Bond Girl Bliss
Click the link at the bottom of this post for the entry form. Please type your name or screen name in the Caption or Title field, then choose the link and photo you'd like to enter. Please link directly to your specific blog post with your card in it, and not your general blog url.
Note: If you have problems linking your photo (especially from SCS), try entering the link to your card, then choosing From File instead of From Web. Then just browse to where your picture is saved on your computer to get your thumbnail.


text:
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A VGA port for projector connections in conference rooms is still a business must-have, and a native VGA means no dongles to buy/lose/replace.
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TrackPoint® and 5-button glass TouchPad
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Offering premium performance and sophisticated simplicity, the Lenovo Ultraslim Plus Wireless Keyboard & Mouse offers easy-access controls and high-performance laser precision to help you get more done, comfortably.
Expect nothing short of an outstanding value. Completely re-designed for a new generation of ThinkPads, snap into the ThinkPad Pro Dock with an included ThinkPad AC Adapter and get connected to power, your Local Area Network/Internet and workplace accessories such as up two external displays, keyboard, mouse and printer so you can get more done faster.
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The Casual line of ThinkPad carrying cases offers a look that is relaxed and carefree, yet undeniably professional. The line includes a tote, messenger, backpack and a topload each made of high-quality twill that is soft-to-touch and durable enough for everyday use. These light weight cases have protective compartments to safely store your ThinkPad computer and tablet, as well as additional pockets and pen loops to organize all your travel essentials.
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It's the most personal part of your PC. The spacing and height of our keys are specially designed to be comfortable to use and to increase accuracy.SEE MORE REASONS


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Celebrate the best of humanity.
Welcome, guest!
by Isabella from Loveland
Clayton 'Peg Leg Bates' (https://myupstatenewyork.wordpress.com/2012/08/02/famous-upstaters-peg-leg-bates-vaudevilles-greatest-star/)
Although I have many heroes one that is truly inspiring is Clayton Bates, otherwise known as "Peg Leg Bates," the famous one-legged tap dancer. Clayton lost his leg at age 12 in a cotton gin accident and he wanted to be in the army like his uncle but his disability stopped him..but it didn't stop his faith.
Being an African American born in 1907 he faced a lot of racism, but he kept his head in the stars and his "foot" on the ground and through this act he showed great faith and self confidence.
Sculpture of Peg Leg Bates by Joe Thompson (Photo by Mike Nice (www.greenvilledailyphoto.com))
By teaching HIMSELF to tap dance Clayton showed undeniable perseverance. I know - I am learning to tap dance myself and I know that it is hard, and I could never imagine how hard it would be to teach myself, let alone with one leg!
Tap Talk (www.melbasdance.com)
Now he not only was a tap dancer but he also was a very busy man. He owned Peg Leg Bates Country Club, performed for the Ed Sullivan show 58 times, and he even performed for the King and Queen of England 2 times!
U.S. Route 209, New York (roadsideamerica.com)
U.S. Route 209 in New York has been named the "Clayton Peg Leg Bates Memorial Highway" after him. This shows that I'm not the only one who thinks he is a hero, and that he is a man worth remembering.
Written by
Isabella from Loveland
Last changed on: 3/12/2015
Clayton Bates
American Tap Dance Foundation
Clayton Bates
MY HERO story by Maya from Midlothian
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established the Sundance Film Festival to effect change in the world.
Robert Shetterly
paints portraits of Americans who have acted with courage to do the right thing.
Robert Wise
was a filmmaker, teacher and mentor.
Roberto Arevalo
of The Mirror Project
teaches kids TV production.
Robin Altman
is an active participant in the arts community in the Laguna Beach area.
Robin Williams
gave love, laughter and joy to all.
Ron Kovic
makes art an affirmation of life.
Ron Kovic
has worked for peace for over four decades.
Rudolph Nureyev
was a legendary Russian ballet dancer.
Ruth Posner
'I don't think of all the misery but of the beauty that still remains.'
Salvador Dali
one of the most recognized Spanish surrealist artists
Scott Joplin
composed operatic and ragtime music and influenced early jazz.
September McGee
is an artist and a teacher.
Simon Velez
is a prize winning architect who uses sustainable materials.
Stephen Colbert
uses his connections and wit to help others.
Stephen Joseph
uses art to protect the land.
Stephen Sondheim
composed popular Broadway musicals.
Steven Spielberg
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Stevie Wonder
a musician who has overcome great obstacles
Tadao Ando
is a world-famous Japanese architect.
Taron Lexton
applies his filmmaking talents to advocate for human rights.
The Mirror Project
The Mirror Project continues to teach kids cinematography.
Tom Shadyac
is a gifted and accomplished writer and filmmaker who gave up everything to understand the human spirit.
Tomihiro Hoshino
creates captivating art and poetry despite paralysis.
Veronica Tennant, the Prima Ballerina
is an accomplished Canadian dance film producer and director.
Vincent Van Gogh
remains one of the world's most renowned artists.
Violence plunged after he brought the arts to a Tijuana neighborhood
Vojko Gasperut
creates beautiful paintings despite his paralysis.
Walt Disney
was both a visionary artist and an entrepreneur.
Wang Yani
has been painting since
she was three
years old.
Will Smith
is a musician and actor whose roles are often heroic and funny.
Wilson Bentley
was the first person to photograph a single snowflake.
is an internationally recognized artist and marine life conservationist.
is a chorus of senior citizens that sings classic rock and pop songs.
Youssef Chahine
Argentine painter, sculptor, writer, and inventor of imaginary languages
Yuri Mason
shared her love of life and peace through the power of her paintings.
Zaha Hadid
is the first woman to be awarded the Pritzker Architecture Prize.
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Vincent was nominated in 2008 for a SAG Award along with his Mad Men costars for Outstanding Performance by an Ensemble in a Drama Series.
His first movie role was a minor part in the 1993 film Untamed Heart.
Vincent has worked with his favorite actress, Monica Keena, in two films. They were both in Crime and Punishment in Suburbia and Strike!.
Vincent had said he enjoys rock climbing.
Some of Vincent's movie roles include: The character Pick in the film Alpha Dog and Thomas Caffey in the movie The Unsaid.
Vincent loves books and poetry. He enjoys George Orwell, Ranier Maria Rilke, Charles Dickens, and John Steinbeck.
Vincent dated actress Rachael Leigh Cook.
Since the age of nine, Vincent has been a chain smoker.
Vincent appeared as Connor in 27 episodes of Angel, 22 as a show regular and 5 as a guest star.
Vincent has stated that he enjoys being lazy.
Vincent is a fan of William Shakespeare.
His major in college was History.
Vincent is a fan of the band "Tool."
Vincent prefers watching 'The Discovery Channel' over regular scripted programs.
Vincent was named after the artist, Vincent Van Gogh.
Vincent is a fan of the band "Alice in Chains."
Vincent was trained in fencing.
Vincent is 5' 11" tall.
Vincent rarely watches television.
Vincent attended UCLA.
His favorite actress is Monica Keena.
His favorite board game is Monopoly.
Vincent failed the 9th grade.
Vincent has four sisters and one brother; he is the youngest child of six.
Vincent trained in martial arts in preparation for the part of Connor on TV show Angel.
Vincent: Yeah, I mean there's some of me in that character, but no I'm a very light hearted person in most circumstances. I should say, on the exterior, I'm a very light person. I tend to like to make jokes and I'm sarcastic and I'd like to think I'm witty and funny and I don't take myself seriously. But I also have that other side of me when I feel I've been improperly treated or if I feel I'm not happy with something I've done and I'm not happy with myself. I think everyone has more than one side to them.
Vincent: Oh my god! The people are great and I was terrified that I was going to show up and meet this bunch of nerdy people, but they're great! They're regular people, they're lovely people. You know, 99% of them were completely respectful of our time and personal space and are really good people who like the show a lot, and that's terrific. It's just that its not something I encounter a lot. You know, I live a life of anonymity and to be in a situation where thousands of people know who you are by sight, it's a unique experience, and quite a rush at times and quite a burden at others so it's a mixed blessing.
Vincent: I'm watching my first episode, and they're all talking about what's to come, something very dangerous--the destroyer. And then I appear, this little Peter Pan gone bad. And even Amy Acker is like, 'Yeah, I don't look so thin now, do I?'.
Vincent Kartheiser: For some reason I think words don't quite work when you're trying to explain the facts of life and what it means to be a suffering human.
Vincent Kartheiser: I'm with my mom so much that it's like if we go out to eat somewhere, we probably say ten sentences to each other. We're like an old couple. There's nothing that we have to say. It's okay to sit there and just be.
Vincent Kartheiser: I was such a nerd when I was younger and I may look different now and girls may like me, but I still feel like that nerd.
Vincent: I think it's healthy to hate. I think it's extremely healthy, because without hate, you cannot see love.


text:
By Joe Brewin
Confidence is key to the success of Andrey Arshavin, according to Arsène Wenger.
Arshavin has found himself in and out of the Arsenal first team this season as his manager rotates his strike force.
The Russian hit the winner in the Gunners' Premier League victory over Swansea two weeks ago and Wenger has backed the diminutive forward to continue in the same vein.
"I think he's had a good start to the season," said Wenger. "Gervinho was a little bit in front and he did very well, and he [Arshavin] is a bit in and out. Maybe you will see him tomorrow.
"I rotate more the strikers than anybody else and Andrey, you cannot fault."
Wenger believes the Russia captain has an unfair reputation in certain quarters and remains adamant his No 23 is a model professional.
"There is a difference between the image that Andrey has and who he is really is," said the boss. "He is a very conscientious hard-worker in training.
"Sometimes he has an image a little bit of a non-caring person and that is not at all [the case]. He is 100 per cent a professional player.
"I think what he needs more is confidence. He had a difficult end of season last year and I believe since a few weeks now, he is in very good form."Copyright 2017 The Arsenal Football Club plc. Permission to use quotations from this article is granted subject to appropriate credit being given to www.arsenal.com as the source


text:
Irwin, you took some really nice pictures there man! I'm not sure what some of those are, do you? One almost looks like golden rain, another red bud, but I don't know, could be some type of fruit tree or ornamental....JP
Fantastic blooms! Are those all nectar producers? The unusually cold weather here has knocked back the tropical blooms, but lots of pollen is coming in from native trees.Scott


text:
Fine Arts
Online Bachelor's Degree in Humanities from an Accredited Leader in Distance Learning
Next steps:
The Bachelor of Arts in Humanities (Fine Arts concentration) offers you the opportunity to focus on creative disciplines such as art, music, and theatre.
Fine arts classes are offered as part of a well-rounded humanities degree that fosters skills you can market in almost any industry. As one of the nation's few all-online humanities degree programs, Excelsior College provides the flexibility and convenience that working adults need.
A national leader in online education, Excelsior addresses the challenges that often prevent working adults and nontraditional students from completing a fine arts education. Here's how it works:
- We offer online courses on a rotating eight-week schedule, so you can schedule humanities and fine arts classes to fit within your work and family routines.
- You get maximum transfer credit for previously completed college courses, reducing your Excelsior course load and enabling you to graduate in less time.
- You can get additional transfer credit for eligible forms of military education and professional training.
- We help you save money via affordable tuition and fees. (Visit our tuition page to get the hard numbers.)
- Our outstanding academic advisors will provide you with all the support you may need.
Learn more about the special benefits for working adults in Excelsior's BA in Humanities degree program. (links to portal page, to be created by Noel Levitz, that outlines transfer policies, credit by exam, etc.)
An Online Humanities Degree from an Accredited Institution
Excelsior College is accredited by the Middle States Commission on Higher Education. (Learn more about the MSCHE accreditation and programs specialized accreditations on our accreditation page.)
Our online humanities degree program and fine art classes adhere to high academic standards. Courses are taught by experienced faculty from leading institutions around the country. Although you'll work independently, Excelsior's online portal enables you to collaborate with classmates in the humanities degree program.
The versatile humanities degree carries value among employers in every sector. It can help you advance your career in a wide range of fields, including business, law, government, journalism, education, and nonprofit work.
Minimum of 120 credits: 60 credits (21 in upper level) in arts and sciences and 60 credits (9 in upper level) in applied professional courses, additional arts and sciences courses or a combination of both. Concentration: minimum of 33 credits, with at least 18 at the upper level including a 3 credit capstone course and a minimum 2.0 GPA required. See the credit requirements in chart form.
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text:
Sick at home with a sinus infection..finally got around to posting this up. It's an awesome camp recipie.
This recipie was adapted from Paula Deans Fritters. We use mild jalapenoes instead of bell peppers.
3 cans Black-eyed peas thoroughly drained and mashed
1 egg beaten
1 lb. bacon diced and crisped (reserve bacon fat)
1 cup (jar not fresh) jalapenoes finely diced
2-4 tbsp. flour
2-3 cups flour
First, drain the peas. use a wire mesh collander and gently tap it on the edge of the sink or a bowl.. you want to remove as much liquid as possible so the fritters will not fall apart.
dice bacon and crisp,
Next, mash up the peas,
Add in the egg,
dice the jalapenoes,
Add the bacon and the peppers & mix it up.
Add 2-4 tbsp. of flour 1 at a time until it sticks and you can form small 3" patties.
once each patty is formed, place in a shallow bowl of flour and dust each side.
Fry in bacon grease, 2-3 min on each side; med.-high heat.
Enjoy! These little fritters are AWESOME!! goes well with cajun duck kabobs as a side dish at the camp.


text:
Hi there,
I'm a new user and have been on here for a few hours now and soooooo many of my questions have been answered already, so thank you for that already!
However, for the sake of my current circumstance, and upon noticing that there aren't any posts out there asking about this already so may be of help to others on the forum, I thought I would ask the question.
After completing the online application forms for the CELTA or Trinity course, which asks grammar questions, class room scenario questions, personal passions, etc, you get invited in for a pre-course interview, and after that the course give you a 'yay' or 'nay'.
My question is this: What is the format of these interviews?
...and what do they specifically ask during the interview? Is there a common format? How is one best to prepare for it?
My interview is next week at an establishment in Oxford that runs the Trinity TESOL course.
Help would be sincerely appreciated!


text:
Choose the living option that works best for you!
Take a virtual tour of Perry Hall to see 360° panoramic views of the suites and lounges.
Double Suite
- two bedrooms
- two students per bedroom
- shared living room
- $3,100 per student per semester
Single Suite
- four bedrooms
- one student per bedroom
- shared living room
- $3,900 per student per semester
Super Single Suite
- two bedrooms
- one student per room
- shared living room
- $4,100 per student per semester
Required Meal Plan
- Students living in campus housing are required to purchase a meal plan.
Security Deposit
- $250
When you live on campus you can
- Stay in shape with our state-of-the-art Fitness Center.
- Walk or jog the peaceful wooded trails of Spencer Crest Nature Center.
- Have easy access to all of our student clubs and organizations.
- Get involved with Red Barons athletics (varsity or intramural).
- Enjoy the convenience of meals served next door in the Commons!
- Breathe easy in our tobacco-free environment.
Dorm? Residence Hall? Student Housing?
Whatever you choose to call it, living on campus in Perry Hall gives you many advantages.


text:
Centercal Properties, LLC Brand - hours and store locations
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Students serve public interest through fellowships
July 05, 2012
DETROIT – Fifteen Wayne Law students are spending the summer working with a variety of agencies and legal clinics in Michigan and beyond, thanks to the support of the 2012 Public Interest Law Fellowships.
The Wayne State University Law School created the fellowships in 2009 to give students experiential education before graduation; to help ease their financial stresses; and to help the organizations where the students are doing public interest jobs. Fellowship recipients were selected by a committee of Wayne law faculty members, staff and alumni.
Assistant Professor Brandon Hofmeister directs the PILF program, which serves several goals, he said.
"First, in public interest careers in particular, past work experience and networking is crucially important for students hoping to land fulltime jobs after graduation," he said. "Because many public interest non-profits and government agencies have very strapped budgets, they often cannot afford to pay summer legal interns. Providing funding allows our students to take these otherwise non-paying jobs without incurring more debts to pay their summer living expenses. The internships give our students valuable work experience and connections, which help them ultimately to meet their goals of obtaining full-time public interest jobs.
"Second, Wayne State University Law School is a public, urban law school which is committed to broadly serving the public interest. The PILF program enables our outstanding students to give voice and legal expertise to populations and causes that are often underserved by the legal community."
The financial aid that goes with the fellowship varies each year, and is applied toward recipients' winter semester financial aid. For 2012, the amount is $4,000 per student.
"So far, the work is great," fellow Lynn Bartkowiak said. The Livonia resident is working at Lakeshore Legal Aid in Southfield. "In the past few weeks, I've been able to work directly with over 30 clients, assisting them alongside the attorneys on staff. I've spoken with opposing parties and counsel, corresponded with other parties and agencies and performed a variety of legal research.
"My favorite part of the work is speaking with the clients and having the opportunity to provide some comfort in light of their legal issues. The most difficult part of the job is bearing bad news or telling clients that we are unable to assist them with their problem," Bartkowiak said. "Some of the situations are heartbreaking to me."
She plans to pursue some type of public interest work in Detroit after graduation.
Working with the lawyers on the Sexual Assault Unit at the Wayne County Prosecutor's Office has been a major learning experience for Ana Stutler, who lives in Detroit. "I have definitely been able to learn a lot about what it takes for a case to go from receiving a warrant request from the police all the way up until sentencing," she said. "I've been able to help attorneys with details every step of the process."
But it's not easy.
"One of the greatest challenges has been learning the balance of seeking justice and fairness for both the defendant and the victim, and dealing with all of the human emotions that are associated with the trial process for both the defendant and the victims," Stutler said.
She chose to work on the sexual assault legal team "because I have a passion for helping those affected by sexual crimes, and also wanted to explore criminal prosecution to see if it would be an area that would suit me in the long term," she said. "I'm not sure specifically what I'll be doing (after graduation), but I know I want to be working in public interest law.
"I chose Wayne Law because I wanted to be in Detroit, and when evaluating my options, Wayne Law provided the best education, financial aid, opportunities for students, and has specific programs — like this fellowship — to encourage public interest work."
Kanika Suri, who lives in Royal Oak, is excited about her summer work with the Michigan Immigrant Rights Center in Ann Arbor.
"MIRC is a wonderful organization to work for," Suri said. "I have been able to deepen my understanding of immigration law, have direct contact with clients within the system and strengthen my Spanish-speaking skills. It is a very hands-on approach to learning. This is a unique skill that one necessarily does not get attending lectures and reading case law. It has cemented the fact that I do want to pursue a career in this field."
She enjoys meeting and talking to clients, and her ability to speak Spanish has made her an asset to the center, where many of the clients are Spanish-speaking.
"Most of our clients are victims of domestic violence, and it is very difficult to hear detailed stories of the physical, mental and economic abuse some have suffered," Suri said. "However, I remind myself that we are helping them escape an awful past and giving them a chance of a safer, freer future."
Fellow Robert Johns of Detroit, who plans a career in environmental law, is serving his internship with the Michigan Attorney General's Environment, Natural Resources and Agriculture Division, and he's found the work to be "exciting" as well as educational.
"So far, I've worked on cases involving invasive species, air pollution, wetlands, hazardous chemical dumping and even a Native American matter," he said. "The attorneys in my office are extremely helpful and willing to share their years of experience in everything from trial strategies to the politics of working for a state department."
2012 Public Interest Law Fellowship recipients:
· Lynn Bartkowiak, Livonia, Lakeshore Legal Aid
· Sarah Douglas, Ann Arbor, Washtenaw County Public Defender
· Tasnim Hassanali, Ferndale, Advocates for Informed Choice
· Robert Johns, Detroit, Michigan Attorney General's Office
· Melissa Knox, Lincoln Park, U.S. House Judiciary Committee, U.S. Attorney's Office, Eastern District, Michigan
· Nicholas Leonard, Detroit, Michigan Children's Law Center
· Margaret Lukshaitis, Royal Oak, Michigan Poverty Law Program
· Lisa Maskill, Ypsilanti, Lakeshore Legal Aid
· Katie Okonowski, Dearborn, U.S. Environmental Protection Agency
· Nick Ranke, Bloomfield Hills, Michigan Department of Environmental Quality
· Zachary Steffes, Westland, Washtenaw County Public Defender
· Ana Stutler, Detroit, Wayne County Prosecutor-Sexual Assault Unit
· Kanika Suri, Rochester Hills, Michigan Immigrant Rights Center
· Patrick Tully, West Bloomfield, Massachusetts Department of Recreation and Conservation
· Jessica Wayne, Novi, Farmworker Legal Services of Michigan
Wayne State University is a premier urban research institution offering more than 400 academic programs through 13 schools and colleges to nearly 31,000 students.


text:
Following on from our request in the Friday 9th March edition of Cycleclips, it is our pleasure to present the local bike shops that our CTC supporters highly recommend.
Helen Royan from Leeds had this to say about her local bike shop - Dave Rayner Cycles:
"The owner Graham Stubbs is extremely helpful. You can ask the most 'stupid' question and get treated with the same respect as someone who knows what they are talking about. It is a family run business – all cyclists – who only want you to be happy as a cyclist. Help and advice is always available for anything from as small as buying some energy gels or bars to getting the most suitable bike for you. Nothing is too much trouble.
You might think this treatment is standard from bike shops but Graham and his family go above and beyond the realms of being a 'standard' shop. I cannot praise them highly enough. They certainly keep my interest in cycling all year round".
If you want to have your local bike shop represented please leave a comment below.
Did you know that CTC friendly shops offer our supporters a discount of up to 10 per cent?
Please note: remember to check that the bike shop still offers the discount before you visit as terms and conditions may apply.
To view the latest lists and comments, please click on the PDFs below.


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Locatelli-Hoops, S., Sheen, Fangmin C., Zoubak, L., Gawrisch, K. and Yeliseev, A.A.
Notes: The HaloTag® Protein Tag was used to tag, immobilize and purify functional cannabinoid receptor CB2, a GPCR, after induction of expression in E. coli. (4263)
Expand Full Notes »
pFC20K HaloTag® T7 SP6 Flexi® Vector
pFN18K HaloTag® T7 Flexi® Vector
pFN19K HaloTag® T7 SP6 Flexi® Vector
Expand Products »
Deplus, R., Delatte, B., Schwinn, M.K., Defrance, M., Méndez, J., Murphy, N., Dawson, M.A., Volkmar, M., Putmans, P., Calonne, E., Shih, A.H., Levine, R.L., Bernard, O., Mercher, T., Solary, E., Urh, M. Daniels, D. and Fuks, F.
Notes: These authors set out to determine how TET2 and TET3 proteins are involved in epigenetic regulation. To characterize proteins that interact with TET, the authors expressed full-length TET1, TET2 and TET3 as HaloTag® fusion proteins and performed protein pull-downs. They identified novel interactions between all three TET proteins and O-GlcNAc transferase (OGT), which catalyzes the addition of N-acetylglucosamine (GlcNAc) to numerous transcription factors, regulatory proteins and histones to activate or inhibit the target protein or recruit additional proteins. In this paper, they focused on TET2 and TET3, which showed the strongest interaction with OGT. They mapped TET2, TET3 and OGT binding throughout the genome by expressing these proteins as HaloTag® fusion proteins in HEK293T cells, crosslinking the proteins and DNA, then capturing the fusion proteins and associated DNA fragments and performing high-throughput sequencing to show that TET2/3 and OGT colocalize at active gene promoters and were tightly clustered near transcription start sites.
For expression of HaloTag® fusion proteins and controls, HEK-293 cells were plated at 12 ×106 cells in a 150mm dish and grown to 70–80% confluency before transfection with 30µg of plasmid using the FuGENE® HD Transfection Reagent.
To assess whether TET2/3-OGT activity affects the interaction of SET1/COMPASS with chromatin, the authors used bioluminescence resonance energy transfer (BRET). They created a fusion protein consisting of the H3K4 methyltransferase SETD1A and NanoLuc® luciferase as the energy donor and a fluorescently labeled histone H3.3-HaloTag® fusion protein as the energy acceptor. These BRET experiments confirmed that TET2/3-OGT activity is necessary for SET1/COMPASS complex function and showed that TET and OGT activities promote binding of SETD1A, a component of the SET1/COMPASS complex, to chromatin. This binding increases H3K4me3 levels. Thus, the authors' data support a TET2/3-OGT-mediated mechanism for regulating the SET1/COMPASS complex and thus H3K4me3. (4262)
HaloTag® Protein Purification System
FuGENE® HD Transfection Reagent
Protease Inhibitor Cocktail, 50X
Black, J.C., Manning, A.L., Van Rechem, C., Kim, J., Ladd, B., Cho, J., Pineda, C.M., Murphy, N., Daniels, D.L., Montagna, C., Lewis, P.W., Glass, K., Allis, C.D., Dyson, N.J., Getz, G. and Whetstine, J.R.
Notes: The HaloTag® protein tag was used in experiments to identify protein partners of KDM4A that involved in site-specific copy number gain in tumors, specifically at the 1q12h region. Expression constructs were transfected into HEK293T cells using the FuGENE® HD Transfection Reagent. The HaloTag-KDM4A (Cat.# FHC00602) and HaloTag-Suv39h1 (Cat.# FHC09879) expression constructs were obtained from the Kazusa DNA Research Institute (Kisarazu, Japan). Interacting proteins identified included DNA polymerase subunits and members of the minichromosome maintenance (MCM) complex. (4408)
pHTN HaloTag® CMV-neo Vector
HaloLink™ Resin
RQ1 RNase-Free DNase
Galbraith, M., Allen, M., Bensard, C., Wang, X., Schwinn, M., Qin, B., Long, H., Daniels, D., Hahn, W., Dowell, R., and Espinosa, J.
Notes: These authors identified a previously unknown interaction between the transcription factor HIF1A and the cyclin-dependent kinase CDK8 (a component of the Mediator complex) in the regulation of genes associated with cellular survival under low-oxygen conditions. As part of the study, HaloTag technology was used to identify proteins interacting with CDK8 in a colorectal cancer cell line. Specifically, cells were transfected with CDK8 and CDK19 HaloTag fusion constructs obtained from Kazusa Institute. The cell lysates were then used in pulldown assays to capture interacting proteins. The results showed that CDK8 and CDK19 are present in mutually exclusive Mediator complexes. Details of the transfection are as follows: HCT116 cells were plated in 150 mm dishes and grown to 70%–80% confluence before transfection with 30 μg of plasmid DNA using FuGENE HD Transfection Reagent. (4355)
HaloTag® Mammalian Pull-Down System
Urh, M., and Rosenberg, M.
Notes: This paper provides an overview of the many applications of HaloTag® Technology. The authors describe the development of the technology, focusing on it's multifunctional utility for protein imaging, protein isolation and display, and in the study of protein complexes and interactions. They also discuss it's potential to facilitate proteomics research studies across complex biological systems at the biochemical, cell-based and whole animal level. (4325)
HaloTag® Protein Purification System Sample Pack
HaloTag® Mammalian Protein Detection and Purification System
HaloTag® Mammalian Pull-Down and Labeling System
HaloTag® TMR Ligand
HaloTag® diAcFAM Ligand
HaloTag® Biotin Ligand
Neunuebel, M.R., Mohammadi, S., Jarnik, M., and Machner, M.P.
Notes: In this study, the L. pneumophila protein Lem3 was expressed as a HaloTag® fusion protein and purified using HaloLink™ Resin. Lem3 was first cloned into the pFN22K HaloTag® Vector and the resultant HaloTag-Lem3 protein was expressed in Single-Step (KRX) competent cells before purification using the HaloTag® Protein Purification System. Lem3 was cleaved from the HaloLink™ Resin using TEV protease.
HaloTEV Protease
pFN22K HaloTag® CMVd1 Flexi® Vector
Single Step (KRX) Competent Cells
Yuanzheng, H., Xu, Y., Zhang, C., Gao, X., Dykema, K.J., martin, K.R., Ke, J., Hudson, E.A., Khoo, S.K., Resau, J.H., Alberts, A.S., Mackeigan, J.P., Furge, K.A. and Xu, H.E.
Notes: Yuangheng He and colleagues asked how the weak alkaline compound chloroquine (CQ) enhances the anti-inflammatory effects of synthetic glucocorticoids like dexamethasone, which are used to treat a host of inflammatory and autoimmune diseases. In the process they explored the intersection of lysosomal degradation pathways and glucocorticoid receptor signaling. They used Dual-Glo® Luciferase Assay System to look at glucocorticoid receptor-mediated (GR) activation and repression of reporters in AD293 cells under a variety of conditions (presence or absence of CQ; stripped serum, loss of lysosome synthesis, inhibition of V-ATPase, etc). They also used HaloTag® protein fusions to observe the fate of GR populations in the presence or absence of CQ and in the presence or absence of compounds that impair proteasome function. Live-cell imaging of GR-HaloTag® protein fusions revealed a dynamic association of the GR with lysosomes. The authors showed that glucocorticoid signaling is regulated by lysosomes. (4203)
Dual-Glo® Luciferase Assay System
Maruyama, A., Nishikawa, K., Kawatani, Y., Mimura, J., Hosoya, T., Harada, N., Yamamato, M. and Itoh, K.
Notes: These authors first used a FLAG-tagged protein (nfr2) with a HeLa Nuclear extract and captured interacting proteins via SDS-PAGE and in-gel digests of bands to identify (Krüppel-associated box)-associated protein 1 (KAP1) as a potential interacting partner. Human KAP1 was purchased as a HaloTag® CMV Flexi® Vector from Kazusa and used in a Mammalian PullDown scenario (with HaloLink™ Resin) to demonstrate interaction between the two proteins. A reporter assay was used to show that KAP1 facilitates Nrf2 transactivation in a dose-dependent manner. The authors defined the interaction sites using GST-tagged nrf2 and various forms of KAP1-HaloTag® Fusions expressed in TNT® SP6 High-Yield Wheat Germ Extract. GST-tagged proteins were expressed in E. coli and bound to glutathione-Sepharose beads. These bound proteins were mixed with the KAP1 from the cell-free expression system, incubated for 4 hours at 4°C, washed and stained with the HaloTag® TMR Ligand for 30 minutes. The proteins from the pull-down assay were subjected to SDS-PAGE and the HaloTag® proteins detected by phosphorimaging and the GST proteins by Coomassie Brilliant Blue Staining. A two-hybrid system consisting of the pRL-TK Vector with a firefly luciferase reporter with Gal4 UAS, mouse Nrf-2 N-terminal domain and KAP1 was also used. The vectors were transfected into Nrf2 knockout MEFs for 4 hours then incubated for 36 hours before luciferase expression was determined using the Dual-Luciferase® Reporter Assay System. (4123)
Anti-HaloTag® pAb
Dual-Luciferase® Reporter Assay System
pFN21A HaloTag® CMV Flexi® Vector
pGEM®-T Easy Vector System I
pGEM®-T Easy Vector System II
pGL3-Basic Vector
pRL-TK Vector
Neklesa, T.K., Tae, H.S., Schneekloth, A.R., Stulberg, M.J., Corson, T.W., Sundberg, T.B., Raina, K., Holley, S.A., and Crews, C.M.
Notes: These authors investigated whether HaloTag® technology could be used to specifically target and degrade intracellular proteins. They developed a method to degrade specific proteins of interest using a small molecule by tagging the HaloTag® protein with an adamantyl moiety. The hypothesis was that appending a hydrophobic residue to a protein surface domain would mimic partial denaturation and induce proteasomal degradation. They first used a HaloTag®-luciferase fusion protein to determine the biological activity of various small molecules that enhanced hydrophobicity. After selecting the small molecule that reduced luciferase activity in HEK293 cells, they went on to test their hypothesis in various in vitro and in vivo models. They were able to demonstrate degradation of various HaloTag®-linked transmembrane proteins expressed in HEK293 cells, to show degradations of target proteins in Zebrafish, and to show degradation of the HRAS oncogene product both in NIH3T3 cells and in a mouse tumor model. (4125)
Steady-Glo® Luciferase Assay System
Ohana, R.F., Hurst, R., Vidugiriene, J., Slater, M.R., Wood, K.V. and Urh, M.
Notes: The authors of this paper demonstrate the utility of the HaloTag® protein purification system for purifying functional proteins from mammalian cells. To this end five kinases were cloned into HaloTag® vectors, expressed in and purified from HEK293T cells. To demonstrate functionality of the purified recombinant kinases, activity was measured using the appropriate ADP-Glo™ Assay Kinase Enzyme Systems. (4145)
ADP-Glo™ Kinase Assay
ADP-Glo™ Kinase Assay + EGFR Kinase Enzyme System
ADP-Glo™ Kinase Assay + PKCγ Kinase Enzyme System
ADP-Glo™ Kinase Assay + SRC Kinase Enzyme System
Hattori, T., Müller, C., Gebhard, S., Bauer, E., Pausch, F., Schlund, B., Bösl, M.R., Hess, A., Surmann-Schmitt, C., von der Mark, H., de Crombrugghe, B. and von der Mark, K.
Notes: To study the role of the transcription factor Sox9 in the transition from cartilage to bone in newborn mice, the authors performed chromatin immunoprecipitation using the HaloCHIP™ System. Primary rib chondrocytes were transfected with a vector expressing full-length Sox9 with a HaloTag® protein tag, then proteins and DNA were cross-linked. DNA was isolated and sonicated, and the Sox9:DNA complexes were precipitated using the HaloLink™ Resin. The precipitated DNA then was amplified by PCR to determine that SOX9 binds to the SRY sites in the Vegfa gene. (4054)
HaloCHIP™ System
Kei Yamaguchi, Shinichi Inoue, Osama Ohara and Takahiro Nagase
Notes: The authors used the Halotag® Labeling Technology in pulse-chase experiments. They pulse labeled proteins in cultured mammalian cells. Using the HaloTag® Technology, they were able to monitor the degradation of the labeled protein, Smad1, that was induced by coexpression of Smurf1. They conclude that the HaloTag® Technology could be used to monitor the regulation of SMAD1 degradation. (4055)
HaloTag® Succinimidyl Ester (O4) Ligand
HaloTag® TMRDirect™ Ligand
Ohana, R.F., Encel, L.P., Zhao, K., Simpson, D., Slater, M.R., Urh, M., Wood, K.V.
Notes: The authors note that while many protein fusion tags are available to assist in purifying functional, recombinant proteins in soluble form and adequate amounts, none of the available tags are ideal when applied to the diversity of proteins studied. Available tags are often best-suited to specific aspects of the overall process, be it expression, solubilization, protein capture, etc. HaloTag was designed to address multiple features. The authors demonstrated that HaloTag provided enhanced expression and solubility in E. coli, with efficient protein purification and labeling for screening and quantitation. Enhanced solubility coupled with covalent capture gives HaloTag advantages over conventional affinity fusion tags, demonstrated by the successful purification of a wide variety of proteins, including low expressers, with higher yield and purity compared to the other tested tags. Covalent capture coupled with efficient tag removal to release the target protein, provides higher purity by eliminating tag contaminants. Using full-length cDNAs encoding 23 human proteins of varying size and function, the authors compared the efficacy of HaloTag as an expression and purification tag to the frequently used solubility enhancers MBP and GST. As reported previously, the three larger tags (HaloTag, MBP and GST) provided higher total and soluble expression compared to the smaller His6tag. In comparing soluble protein expression levels for the three larger tags, it was found that HaloTag solubilized 74% of the 23 human proteins examined, compared to 52% for MBP and 39% for GST. G2681 was one of the T7, bacterial promoter-based Flexi vectors used. (4005)
Schröder, J. Benink, H., Dyba, M. and Los, G.V.
Notes: Traditionally light microscopy resolution has been limited by the diffraction of light. However several new technologies have emerged that partially overcome that limitation. One of these stimulated emission depletion (STED) microscopy is now commercially available and has been integrated into confocal microscope platforms. Because STED depends on fluorescent markers that fulfill specific spectroscopic needs, its uses have been limited. The authors of this paper demonstrate successful high resolution of β1-integrin-Halotag®-fusion protein distribution using STED microscopy. Use of the HaloTag® technology allows researchers to create a reporter that can be labeled with STED-optimized fluorescent tags. (3955)
HaloTag® Alexa Fluor® 488 Ligand
Hurst, R., Hook, B., Slater, M.R., Hatrnett, J., Storts, D.R., and Nath, N.
Notes: These authors compared 6 different detection strategies for protein-protein interactions on protein arrays. They expressed HaloTag® labeled bait proteins in a cell-free expression system, and captured these bait proteins onto coated glass slides using the HaloLink™ Array System. They then compared detection strategies using prey proteins labeled as follows: 1)35S methionine, 2) fluorescence (BODIPY-FL) and 3) biotin labeling of lysine residues using modified Lys tRNA, 4) chemical labeling after expression, 5) HaloTag® fusion, and 6) N-terminal FLAG tag. The authors evaluated signal:background ratios, adaptability to high-throughput screening, and ease of use. (3999)
pF1A T7 Flexi® Vector
HaloLink™ Array (TnT® SP6 Wheat Germ) Two Slide System
TnT® SP6 Quick Coupled Transcription/Translation System, Trial Size
FluoroTect™ GreenLys in vitro Translation Labeling System
Transcend™ tRNA
Kardon, J.R., Reck-Peterson, S.L. and Vale, R.D.
Notes: These authors expressed recombinant dynactin and dynein in Saccharomyces cerevisiae and investigated their interactions in motility assays. They created a c-terminal Halotag-Dynactin fusion, and were able to site-specifically label the fusion protein with the fluorescent dye tetramethylrhodamine (TMR). They studied the effect of the purified dynactin fusion protein on the motility of dynein complexes using total internal reflection fluorescence microscopy. Dynactin alone did not interact with microtubules. However, when coincubated with recombinant dynein, the TMR-labeled dynactin moved processively along microtubules. The authors then used truncation mutants of dynactin to identify the region of the dynactin molecule required for localization and enhanced processivity of dynein. (3960)
Hasegawa, Y., Honda, A., Umezawa, K., Niino, Y., Oka, K., Chiba, T., Aiso, S., Tanimoto, A., Citterio, D., and Suzuki, K.
Notes: These authors describe a method for cell-surface labeling with two genetically expressed tags, a biotin acceptor peptide (BAP) and HaloTag (HT). BAP and HT were fused with the N-terminus of the mouse Ig κ-chain leader sequence to direct them to the secretory pathway, and the c-terminus of the platelet-derived growth factor receptor transmembrane domain to anchor them to the plasma membrane. Fluorescent proteins were fused with BAP and HT to create the two distinct fluorescent transmembrance proteins BAP-YFP-TM and HT-CFP-TM. The constructs were introduced into HeLa cells and imaged simultaneously. The authors then demonstrated in vivo imaging by injecting the doubly-tagged HeLa cells into nude mice and imaging with near infrared fluorescent probes, and with magnetic resonance probes. (3998)
Yamakawa, Hisashi
Notes: The authors use the Flexi® Cloning System to convert their cDNA clones to expression-ready clones. They wanted clones that could be used for comprehensive analysis with the HaloTag® Technology. They also describe a method of transferring ORFs between Flexi® Vectors in a 96-well plate format. They also used Wizard® SV 96 Plasmid DNA Purification, Wizard® SV PCR Clean-Up, and Wizard® SV Gel and PCR Clean-Up Systems. (4056)
Flexi® System, Entry/Transfer
Flexi® System, Transfer
Wizard® SV 96 PCR Clean-Up Start-Up Kit, 110V Electrical (8 x 96 preps, manifold and free vacuum pump)
Wizard® SV 96 PCR Clean-Up Start-Up Kit, 220V Electrical (8 x 96 preps, manifold and free vacuum pump)
Wizard® SV 96 PCR Clean-Up Start-Up Kit, 100V Electrical (8 x 96 preps, manifold and free vacuum pump)
Wizard® SV 96 PCR Clean-Up System
Wizard® SV 96 Plasmid DNA Purification System
Wizard® SV Gel and PCR Clean-Up Start-Up Kit, 110V Electrical (500 preps, manifold and free vacuum pump)
Wizard® SV Gel and PCR Clean-Up Start-Up Kit, 220V Electrical (500 preps, manifold and free vacuum pump)
Wizard® SV Gel and PCR Clean-Up Start-Up Kit, 100V Electrical (500 preps, manifold and free vacuum pump)
Wizard® SV Gel and PCR Clean-Up System
Danette D Hartzell, Nathan D Trinklein, Jacqui Mendez,
Nancy Murphy, Shelley F Aldred, Keith Wood and Marjeta Urh
Notes: The HaloCHIP System was used to measure CREB binding as an alternative to the ChIp method. (4057)
Du, X., Rosenfield, R. and Qin, K.
Notes: The authors used a HaloTag® vector and the HaloCHIP™ System to identify a KLF15 binding site in the HSD17B5 promoter. (4059)
Yung Ming Lin, Chia Ling Chung, and Yu Sheng Cheng
Notes: Human boule (BOLL) gene was subcloned into the HaloTag® pHT2 Vector and transformed into JM109 and HeLa cells. (4060)
Svendsen, S., Zimprich, C., McDougall, M.G., Klaubert, D.H., and Los, G.V.
Notes: This paper demonstrates use of HaloTag® technology to study expression, trafficking and translocation of an integrin-HaloTag® fusion protein. The authors fused the Halotag reporter protein to truncated integrin. They then labeled live cells with different cell-permeant and impermeant ligands and followed spatial separation of plasma membrane and internal pools of the integrin-HaloTag® protein. (3912)
HaloTag® PEG-Biotin Ligand
Nagase, T., Yamakawa, H., Tadokoro, S., Nakajima, D., Inoue, S., Yamaguchi, K., Itokawa, Y., Kikuno, R.F., Koga, H. and Ohara, O.
Notes: These authors used the Flexi® Vector System to prepare ORF clones encoding 1929 human genes and to transfer a subset of these clones to various expression vectors for further analysis. They created HaloTag® fusion proteins and examined expression of these proteins in vitro and in COS7 and HEK293 cells. They also performed comparisons between the Flexi® System and Gateway® cloning system, specifically examining the effects of flanking sequences on protein expression in in vitro translation systems and confirming that the cellular localization of the HaloTag® fusion proteins was consistent with results obtained using GFP-fusions. (3800)
GloMax® 96 Microplate Luminometer w/Dual Injectors
Monster Green® Fluorescent Protein phMGFP Vector
pF1K T7 Flexi® Vector
pGL4.10[luc2] Vector
T7 RiboMAX™ Express Large Scale RNA Production System
TnT® SP6 High-Yield Wheat Germ Protein Expression System
Tatematsu, K., Yoshimoto, N., Okajima, T., Tanizawa, K., and Kuroda, S.
Notes: RBCK1 is a RING-IBR (ring in between ring fingers) protein previously shown to have transcriptional activity and to bind protein Kinase Cβ. This paper demonstrates that RBCK1 also possesses ubiquitin ligase E3 activity. Both FLAG-tag and HaloTag® labeled proteins were used to demonstrate this activity. To demonstrate the interaction between RBCK1 and ubiquitinated proteins, HaloTag®-ubiquitin and FLAG-RBCK1 were coexpressed in HEK293 cells in the presence or absence of a proteasome inhibitor. The anti-FLAG immunoprecipitates isolated from these cells were analyzed by SDS-PAGE and Western blotting using anti-HaloTag® and anti-FLAG antibodies and self-ubiquitination of RBCK1 was demonstrated. RBCK2, a splice variant of RBCK1 lacking the RING domain showed no self ubiquitination activity but was demonstrated to interact with RBCK1 in vivo and in vitro, and to inhibit the self-ubiquitination activity of RBCK1 in a FLAG-tag assay. Pulse-chase experiments, using HEK293 cells expressing HaloTag®-RBCK1 with or without RBCK2 and treated with HaloTag®-TMR ligand, were used to show that the half-life of RBCK1 was extended by overexpression of RBCK2. (3874)
Ogawa, M., Shinohara, H., Sakagami, Y. and Matsubayashi, Y.
Notes: The authors of this study investigated the role of the genes CLV1 and CLV3 stem cell renewal and differentiation in self-renewing shoot apical meristem (SAM) in Arabidopsis. CLV1 encodes a receptor kinase that is a negative regulator of cell growth, and therefore cannot be overexpressed in plant cells. CLV3, which encodes a protein that is processed to a 12-amino acid peptide, is believed to be a ligand for CLV1. In this study, the authors made a construct in which the CLV1 kinase domain was replaced with the HaloTag™ protein and expressed the fusion protein in tobacco BY-2 cells. The fusion protein was detected using the HaloTag™ TMR ligand. They next incubated membrane extracts from wildtype and transgenic BY-2 cells (expressing the HaloTag™-CLV1 fusion) with tritium-labeled CLV3. The transgenic cells showed significantly higher CLV3 binding than wildtype, and the authors show that CLV3 specifically labels a 130-kd band that corresponds to the HaloTag™-CLV1 fusion protein, indicating that CLV3 binds the ectodomain of CLV1. (3763)


text:
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text:
Condition: 9/10
Known Problems: There are no problems with this gun. However the two piece barrel is stuck together. im pretty sure you can get it apart if it really bugs you
Asking Price: 350
Shipping Options: Whatever you pay for Unless its a trade
Trades of interest: Not looking for anything in particular just don't want to downgrade
Location of item: Canada, Ontario, N0B2T0
What is Included: Eclipse gun oil, Hex key set, Manual, Planet eclipse Zip case, Outer cardboard box, Spare parts kit, Barrel, and the marker
Edited by KLuvSpeedball, 17 October 2012 - 01:10 PM.


text:
by John Ryan
Probably the least-reported pleasure of professional cooking is the conversation.
Despite the patina of glamour and sophistication that food magazines and cookbooks strive for, the fact is, cooking is a pretty tedious chore. But it's one thing to chop an onion and mince a few cloves of garlic for dinner at home. It's quite another to set up for a crowd.
Everyday, professional cooks punch in several hours before serving time and start slicing, dicing, pounding...and talking with anyone in earshot. These conversations are sometimes as stupid and juvenile as any drive-time radio talk show. But not always. First dates, politics, books, hobbies, vacations...they're great conversational ingredients. And over the weeks, months, and even years that a staff works together, these conversations become an almost tangible mosaic.
Day to day cooking at home leaves very little room to start these kinds of conversations. Too often the object is to get dinner on the table as fast as possible. Or perhaps you can't talk because you are trying a new recipe and need to concentrate.
But another problem is our century's incredibly successful war on chores. The ideal situation for conversation to bloom is to have a long, tedious chore in front of you, such as making ravioli, picking basil leaves for pesto, or rolling tamales. When such chores are done for us, idle talk doesn't even get started.
A true tamale story. Last year I was getting ready to teach a class on tamales. A week or so before the class I went to the butcher a couple blocks away and picked up a pork butt to test my recipe. I wasn't quite happy with the results, so a few days later I got another butt to test some changes. And then, a day or two after that, I went in to pick up two butts for the class.
This time the butcher couldn't resist: "What are you doing with these?" and his buddy chimed in: "Yeah, anyone who buys more than one butt a week... we have to report them to a federal agency."
So I told them about the class. I didn't expect that they'd be interested, but the clerk who'd asked lit up and told me about his aunts who got together every year around Christmas and made tamales. He said that it was a tradition with them. They would spend the day in the kitchen making tamales, talking, and laughing.
And how he'd always go home with a dozen fresh tamales.
Normally I don't write about something as labor-intensive as tamales, but they've become an annual tradition with Margaret and me. Instead of throwing a dress up Christmas party, we'll invite a few friends over, tell them to wear something that'll go with cornmeal, then spend the afternoon and evening making tamales, drinking beers, and talking.
Feliz Navidad!
John & Margaret
John Ryan
Both chef and musician, John Ryan wrote the Just Good Food blog from 1996 through 2001.
This page modified December 2000
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text:
There are so many food blogging events, but so little time. The small hand full of marvelous events that we already enjoy participating in has us scrambling till the very last wee hours of the night to submit our contribution. Procrastinating might be the evil force hiding behind our 2am post writings, but some minds work better under pressure. In our case, it's two. But between two very busy jobs and continuous projects in the works, we blog when we can and that means late into the night. And by the way, make that a double cocktail at 1:23 am to alleviate any writers block!
Ironically, as much as we enjoy drinking and pairing our foods with wine, we've yet to participate in any event focused on wine. Luckily, there are some mighty fine folks out there in the blogosphere who are looking out for our inebriating interests. They are Denise and Lenny, the power couple behind the beautiful and delicious blog, Chez Us. They informed us of Wine Blogging Wednesday, a great event that has wine lovers finding a wine that is fits the theme for that month, then write about their tasting notes. This month's theme has everyone reveling in old world Riesling, which is hosted by Tim over at Wine Cast. A monthly event like this that requires drinking wine is too good to pass up, so we're joining in on the festivities and sharing our riesling notes on a German 2005 Veldenzer Elisenberg Spatlese by Weingut Max Ferd Richter.
Old World Riesling is an exciting theme for us this month because we were planning to pair this old world spatlese with some Vietnamese stuffed squid in spicy fish sauce. Todd has been craving this wickedly delicious, but rather time consuming, squid dish, which has been a staple and favorite on our Viet family-side for so many years. But the time in making our pork stuffed Vietnamese squid is worth every slimy, gut cleaning, ink-sac squirting effort. So we headed down the coastSan Pedro'svarious seafood vendors at the San Pedro Fish Market, searching for fresh, baby squid. A major port town in Los Angeles County, San Pedro is a busy shipping port and harbor that is a great source for fresh, local and international seafood. Here, fresh fish, crab, lobster are brought in daily and to our delight, there was plenty of fresh squid at our stuffing disposal.
This Spatlese was quite tasty! It had a moderate nose of green apple, peach, and a bit of honeysuckle forecasting what was about to be tasted. Upon drinking, those elements were confirmed, along with a moderate sweetness that is typically found in a Spatlese Riesling. The fruit was luscious with juicy peach now dominating the palate, but being complimented by the flora notes 1000 as well as the mildly tartness of the green apple characteristics. The wine was delicious on it's own, but our main intent was to see how it would hold up to the challenging pair of this Viet Stuffed Squid and it's palette dominating dipping sauce of Nuoc Cham. The Nouc Cham has a collage of salty, sour, spicy and sweet flavors, with the spicy being felt long and hard. With a fish sauce this spicy, it's sometimes hard to find a wine that isn't too harsh or clashing, or that just gets buried under the fish sauce's power.
How did this Riesling do? When we eat the squid, we'll wrap it in lettuce, fresh Viet herbs, then dip it in the Nuoc Cham. The mouth is left tingling and salivating for more. This Riesling married perfectly. The juicy fruit and sweetness of the wine drew out most all of the heat as well as rounding out the flavors. The fresh herbs were reflected back into the wine and more flora notes were discovered in the mouth. With the spice on the palate nearly extinguished by the wine, each bite and drink became nearly a virginal taste every time, with only subtle remembrances of the passing nibble lingering on the palate. As with any good pairing, both the wine and the food developed new qualities that were heightened in the dining experience.
As mentioned, these stuffed squids never fail to please, so we decided to share our ways of preparing and cooking these Vietnamese delicacies with a video! One of the challenges of cooking stuffed squid is to thoroughly cook the filling, without drying out the squid. We prepared the squid in two ways, grilling it on the pan and deep frying them with the a panko bread crust coating. For the pan method, we suggest covering the pan with a lid for a minute or two to help cook the filling without rubberizing the squid. Deep frying them with the outer layer of egg wash and panko bread crumbs keeps the squid moist during this quicky fry. Both are two great options for you to play with. Also, you don't have to burn in hell like we did with all these chili's, just add enough to fit your level of heat and spice!
Vietnamese Stuffed Squid Recipe
Yield: Serves 4-6
Total Time: 30 Minutes
One of the challenges of cooking stuffed squid is to thoroughly cook the filling, without drying out the squid. Overcooked, rubbery squid SUCKS!! We prepared the squid in two ways, grilling it on the pan and deep frying them with the a panko bread crust coating. For the pan method, we suggest covering the pan with a lid for a minute or two to help cook the filling without rubberizing the squid. Deep frying them with the outer layer of egg wash and panko bread crumbs keeps the squid moist during this quicky fry. Both are two great options for you to play with. Wrap these squids with some fresh Viet herbs, cool cucumber, lettuce and dip it all in a spicy (optional) fish dipping sauce!
- About 20 baby squid, cleaned & gutted
- 1/2 pound ground pork
- 2 cloves of crushed garlic
- 2 T finely minced shallots
- 1 T fish sauce or soy sauce
- 1 t freshly cracked black pepper
- 1 t sugar
- Oil for pan fry or deep fry
- 1 Egg, beaten ( for Deep fry)
- 2 cups Panko Bread Crumbs ( for Deep Fry)
- Clean squid by removing all innards and the cuttlebone (quill). Set on paper towels to remove excess water.
- In separate bowl, combine pork, garlic, shallots, fish sauce, black pepper and sugar.
- Keep the opening of the squid as wide as possible. Start by stuffing about 1 T of the pork stuffing inside body of squid. Fill to only about 1/2 to 3/4 full of the squid.
- "Sew" a thread through the opening of the squid to hold it together. Using another toothpick, poke holes in the squid body to create "vent" holes during cooking.
Do not OVERSTUFF! The filling will expand during cooking and the squid will shrink as it cooks. You don't want any squid explosions in your pan!
Pan Fry-
- Heat about 2 T of oil in pan to low/medium heat. Place squid on hot pan and as squid starts to cook, slowly rotate the cook so that it cooks evenly on all sides. Cook for about 10-20 minutes, depending on how big your squid is and how much you stuffed it. Continue to poke more holes in squid with a tooth pick to release air and juices trapped inside the squid.
- Make sure the filling is cooked thoroughly. If needed, place a lid on pan for about 1-2 minutes to help keep the steam in to fully cook the squid filling. Remove the lid for the last 5-7 minutes of the cooking process to create a more grilled exterior textures for the squid. Remove from pan and enjoy!
Panko Deep Fry-
- Heat oil to medium heat for deep frying. In small bowl, mix egg. In separate bowl, add panko bread crumbs.
- Dip squid in egg wash, then dredge in panko.
- Gently drop in oil. Fry squid on both sides till filling is thoroughly cooked. Continue to poke more holes in squid with a tooth pick to release air and juices trapped inside the squid. The squid will cook quickly, so be ready to pull it out of hot oil immediately. Blot on paper towel.
Hello! All images & content are copyright protected. Please do not use our images without prior permission and do not re-publish this recipe. Simply link back to this post for the recipe. Thank you.
Recipe Note for Salt: All recipes containing salt are based on kosher or sea salt amounts, not table salt. If using table salt, reduce the amount used to taste.
Serve these squids with some fresh Vietnamese herbs, cool cucumber, lettuce and dip it all in a spicy (optional) fish dipping sauce


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|Concealed Firearms (L-R) CVG & SAT
- A 380. caliber firearm loaded with five rounds and one chambered was discovered strapped to a passenger's ankle after walking through a metal detector at Cincinnati (CVG).
- A loaded 380. caliber firearm with a round chambered was discovered in the rear pocket of a San Antonio (SAT) passenger during Advanced Imaging Technology (AIT) screening.
- A 380. caliber firearm loaded with five rounds was discovered after a passenger walked through a metal detector at Dallas – Ft. Worth (DFW).
|Seal Deterrent (ADQ)
|Burning Book (PHX)
- Two inert artillery shells were discovered in a checked bag at Chicago O'Hare (ORD). Two minors were returning from Europe and had found the shells at a French WWI era artillery range.
- Four inert/replica/novelty grenades were discovered in carry-on bags this week. Two were discovered in Atlanta (ATL), and the others were found at Baltimore (BWI), and Oklahoma City (OKC).
- Two razor blades were detected inside the shoe and wallet of a Cincinnati (CVG) passenger.
- A 6-inch saw blade was detected, concealed inside a bible at Orlando (MCO).
- Three box cutter blades were detected concealed in a shoe inside the carry-on bag of a Baton Rouge passenger.
- A cell phone stun gun was discovered at Omaha (OMA).
- A comb knife was discovered at John Wayne (SNA).
- A lipstick stun gun was discovered at Detroit (DTW).
- A shocking device was detected inside a cane at Billings (BIL).
|Saw in Bible (MCO), Razors in Shoe (BTR), Stun Cane (BIL), Comb Knife (SNA)
|Unfolded Credit Card Knife (MCI)
|Stun Guns Discovered at (L-R) OMA, STT, DTW
|Ammunition (PHX)