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Molecular Basis of Human Cardiac Troponin T Isoforms Expressed in the Developing, Adult, and Failing Heart
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Abstract Cardiac troponin T (cTnT), a protein essential for calcium-regulated myofibrillar ATPase activity, is expressed in the human heart as four isoforms (cTnT1 through cTnT4, numbered in the order of decreasing molecular size). The expression of these isoforms at the protein level has previously been found by us to differ in the normal and failing adult and fetal human heart. In the present study, we have cloned and sequenced four full-length cDNAs corresponding to the four native cTnT protein isoforms and have expressed these cDNAs in an in vitro transcription and translation system. The cDNAs differ by the variable inclusion of a 15- and a 30-nt exon in the 5′ half of the coding region. These cDNAs yielded proteins that comigrate with the native isoforms, cTnT1 through cTnT4. Polyclonal antisera, raised against a synthetic peptide corresponding to the 10-residue peptide encoded by the 30-nt exon, reacted with the two human isoforms largest in molecular size (cTnT1 and cTnT2) and the two largest cTnT isoforms of the rabbit and rat. The isoforms cTnT1 and cTnT2, containing either both peptides encoded by the 30- and 15-nt exons or the peptide encoded by the 30-nt exon alone, are expressed in the fetal heart, with cTnT2 being expressed at a very low level. cTnT4, lacking both of these sequences, is expressed in the fetal heart and is reexpressed in the failing adult heart, whereas cTnT3, containing the 5-residue peptide, is the dominant isoform in the adult heart. The identification and acquisition of these full-length clones that encode the four human cTnT isoforms should prove valuable in analyzing genomic DNA, identifying cTnT mutations in these alternatively spliced coding sequences and their splice sites, and studying the functional role of these isoform sequence differences in the normal and the diseased human heart.
Troponin T is a component of the troponin complex that regulates cardiac muscle contraction.1 2 The complex also contains troponin I, which inhibits thick- and thin-filament interaction, and troponin C, whose binding of calcium disinhibits this interaction. Troponin T binds the complex to tropomyosin and confers onto the myofilaments their calcium concentration–dependent ATPase activity.2 Multiple cardiac troponin T (cTnT) isoforms are expressed in the mammalian heart,3 4 5 6 7 8 9 10 whereas troponin C11 and troponin I12 are expressed as single isoforms in the adult heart. Interestingly, in the human heart four cTnT isoforms are expressed in a developmentally regulated manner.3 This expression in the human heart is affected by heart failure.3
The importance of troponin T in proper muscle function and myofibril formation has become evident through mutational analysis. In insect flight muscle, troponin T mutations produce abnormal myofibrillogenesis and function.13 In humans, Thierfelder et al14 have recently found that affected members of some families with hypertrophic cardiomyopathy have cTnT mutations.
The functional significance of expressing multiple cTnT isoforms is supported by a correlation between peak myofibrillar ATPase activity and isoform expression in donor and failing human left ventricular myocardium.3 This functional importance is further supported by the correlation between certain rabbit15 and bovine16 cTnT isoforms and the sensitivity of their myofilaments to calcium and between rabbit cTnT isoform expression and its myofilament calcium binding.17
As a first step toward understanding the functional significance of the diversity of cTnT isoform expression in the human heart, we have deduced from four full-length cDNAs the amino acid sequences of the four human cTnT isoforms and demonstrated that the products encoded by these four cDNAs comigrate with the four native isoforms. Based on the existence of a single cTnT gene,18 19 our sequence analysis demonstrates that combinatorial alternative splicing of two 5′ exons yields the four human isoforms and suggests that in the mammalian heart combinatorial alternative splicing of these two 5′ exons is the general mechanism for producing the four commonly found cTnT isoforms.3 6 20 In light of previous functional assessments, these sequence differences among the isoforms may play a role in modulating myofilament function.
Materials and Methods
Isolation and Characterization of Human cTnT cDNA
MAb 13-11, a mouse monoclonal antibody that recognizes a cardiac-specific troponin T epitope,6 was used to screen two λ Zap II adult human heart cDNA libraries. Two clones, ≈2 kb and 1 kb in length, were isolated and sequenced by the dideoxy chain termination method21 and automated fluorescent dideoxy chain termination (UNC DNA Sequencing Facility).
The 2-kb clone contained a 54-nt 5′ untranslated sequence and 639-nt coding sequence, whereas the 1-kb clone contained, except for the start codon, an entire cTnT coding sequence and the 3′ untranslated region including the polyadenylation signal. Polymerase chain reaction (PCR) was used to introduce the missing first 3 nt of the coding sequence, yielding a full-length cDNA from the 1-kb clone. A full-length cDNA with the same sequence and three additional full-length cDNAs were obtained by using reverse transcriptase (RT)–PCR with primers designed from the 5′ and 3′ noncoding regions of the consensus cDNA.
RT-PCR and Cloning
Total RNA was isolated from human heart tissue, as previously described.20 RNA was isolated from neonatal cardiac tissue that was removed to surgically repair a congenital heart defect after informed consent was obtained and from fetal heart tissue obtained at the time of therapeutic abortion under protocols approved by Brigham and Women's Hospital Institutional Committee for the Protection of Human Subjects. cDNA was synthesized with oligo dT and AMV reverse transcriptase (Promega), according to the manufacturer's directions. PCR was performed on the newly synthesized cDNAs with Taq DNA polymerase (Promega) and oligonucleotide primers designed from the original cDNA clones (see Fig 1⇓ legend). Samples were denatured at 94°C for 1 minute, annealed to the primers for 2 minutes at 58°C, and extended at 72°C for 3 minutes for a total of 25 cycles. RT-PCR products were separated on gels composed of 3% Nu-Sieve (FMC Bioproducts) and 1% agarose (United States Biochemicals).
To search for heterogeneity in the 5′ end of the troponin T coding region, primers o3150 and o3152 (Fig 1⇑ legend) were used to generate PCR products that were excised from an agarose gel, cleaved at the Xba I restriction site carried by o3150 and at an internal Sau3A restriction site, and cloned into pBluescript KS+ at the Xba I and BamHI sites (Stratagene). Primers o3153 and o2820 (Fig 1⇑ legend), designed from the 3′ region of the full-length clone, were used to generate RT-PCR products that were restricted at internal Sau3A sites and cloned into pBluescript KS+ at the BamHI site. Full-length RT-PCR products were generated by using primers corresponding to 5′ and 3′ untranslated sequences, o3150 and o3155, respectively. The resulting cDNAs were cleaved at Xba I and Xho I sites, carried by the primers, and cloned into Bluescript KS. Positive transformants of all constructs were identified by color selection and sequenced with Sequenase, following the manufacturer's recommendations (United States Biochemicals).
We found the in vitro translational efficiency of the cTnT3 cDNA, which was derived from the library screen and contained 185 bp of 3′ untranslated sequence, including a polyadenylation signal, to be greatly enhanced over the cDNAs generated by RT-PCR. For this reason, we used a Sac I restriction site (nucleotide 417), common to all the isoforms, to construct cDNAs that contained this additional 3′ untranslated sequence and used these cDNAs for in vitro translation reactions.
In Vitro Transcription and Translation
Full-length troponin T cDNAs, encoding proteins cTnT1 through cTnT4, were transcribed and translated in vitro (TnT lysate system, Promega) in the presence of [35S]methionine. The translation products were denatured in sodium dodecyl sulfate (SDS) loading buffer and resolved in a 7.5% polyacrylamide gel.
Western Blots
SDS–polyacrylamide gel electrophoresis was used to resolve the proteins of human fetal hearts, adult donor hearts that could not be used for transplantation, failing adult human ventricular myocardium, neonatal and adult rabbit ventricular myocardium, and fetal and adult rat ventricular myocardium.4 The conditions used in the present study were identical to those described previously,4 except that the polyacrylamide concentration was 7.5%. Proteins were transferred to polyvinylidene difluoride (PVDF) and reacted with MAb 13-11 or polyclonal antisera, as previously described.22 Polyclonal antisera were raised against the synthetic peptide KGGEEDWREDEDE, which includes the sequence encoded by the alternatively spliced 30-nt sequence found in human and rabbit cTnT1 and cTnT2 isoforms20 (Fig 2⇓). Immune complexes were detected on the blots by using alkaline phosphatase–linked goat anti-mouse immunoglobulin antibodies, nitro blue tetrazolium, and 5-bromo-4-chloro-3-indolyl phosphate.
Human fetal ventricular myocardial proteins and the in vitro–transcribed 35S-translated products of the human cTnT cDNAs were resolved in SDS-polyacrylamide gels. After transfer of the proteins to PVDF and development of the color reaction, the membrane was exposed to autoradiographic film.
cDNA Isolation and Characterization
A 2-kb cDNA clone (clone 2.9) was isolated from an adult male human heart λ cDNA library by using MAb 13-11.6 The clone contained 58 nt of the 5′ noncoding sequence and 639 nt of the coding sequence (residues 1 to 213 of cTnT). The remainder of the clone corresponded to an unrelated sequence. A 1-kb cDNA clone was isolated from an adult female human heart λ cDNA library by using MAb 13-11. This second clone encoded residues 2 to 288 of a cTnT isoform (Fig 1⇑) and 185 nt of the 3′ untranslated sequence, including the polyadenylation signal. As anticipated, both isolated clones contained the highly conserved sequence (PRSFMPNLVPPKI), which is the epitope of MAb 13-116 (Fig 1⇑). The consensus between the two cDNA clones was an 867-nt coding region that encodes a protein of 288 amino acids (Fig 1⇑), which matches one and is similar to another previously reported human cTnT cDNA.23 24
To ensure the accuracy of the coding sequence and the deduced amino acid sequence, RT-PCR was used to obtain full-length cDNAs. These full-length clones (see below for identification of other isoforms) confirmed that our consensus sequence and that of Townsend et al23 encode an identical protein while differing by two codons from the sequence reported by Mesnard et al.24 In our sequence and that of Townsend et al, codon 129 is AGA and encodes an arginine, whereas in the sequence of Mesnard et al, the codon is GGA and encodes a lysine. At position 239, AGC encodes a serine in our sequence and that of Townsend et al, whereas Mesnard et al report ACG, which encodes a threonine. RT-PCR, using primer pairs from the 5′ and 3′ untranslated regions, reproducibly yielded cDNA that encoded an arginine at 129 and a serine at 239, as did our clones obtained from library screening.
RT-PCR Identification of Additional Isoforms
Five full-length cDNAs were isolated by RT-PCR. Oligonucleotide primers positioned in the 5′ and 3′ untranslated regions (Fig 1⇑ legend) were used to generate the full-length cDNAs, which were subsequently cloned. Sequencing these cDNAs demonstrated that their length difference was a result of combinatorial alternative inclusion of a 30- and a 15-nt sequence in the coding region. Two clones contained both of the sequences, two contained the 15-nt sequence alone, and one contained neither sequence.
RT-PCR, using primers flanking the 5′ half of the coding sequence, was performed to confirm the heterogeneity that was observed in the isolated full-length cDNAs. Four PCR products of different sizes were obtained. A Pvu II site within the alternatively spliced 15-nt sequence provided a convenient method for the assay of length differences among the PCR products. Over 200 cloned PCR products from five RT-PCR reactions were assayed in this manner: the majority comigrated with the Pvu II–cleaved products obtained from the full-length clone containing only the 15-nt exon. The identity of several clones was confirmed by sequencing. Eight clones contained both the 15- and 30-nt exons, 12 clones contained the 15-nt exon alone, 5 clones contained neither exon, and a single clone contained the 30-nt exon alone. It is apparent by Western blot (Fig 4⇓) that the cTnT isoform containing only the 30-nt exon is rare. A full-length cDNA containing the 30-nt exon alone was generated by ligating this 5′ PCR product to the remainder of the coding sequence from one of the full-length clones. The sequence differences among the four cDNAs are shown in Fig 2⇑.
The 15-nt exon encodes a five-residue peptide, EAAVE, that corresponds to residues 18 to 22 of the predicted amino acid sequence of the consensus cTnT cDNA (Fig 1⇑). This peptide sequence is present in sheep cTnT25 and is similar to the sequences present in cow and rabbit isoforms10 20 (Fig 3⇓).
The 30-nt sequence encodes a peptide, EEEDWREDED, falling between residues 22 and 23 deduced from the consensus sequence (Fig 1⇑). A previously reported RT-PCR fragment of human cTnT contained a 27-nt insertion in the same position.23 In our nine sequenced 5′ RT-PCR clones that contained an additional sequence in this position, the sequence was always 30 nt in length. This cTnT 30-nt sequence encodes a 10-residue peptide that is identical to a peptide encoded by a 5′ alternatively spliced 30-nt exon reported in rabbit20 and similar to the 10-residue peptides encoded by rat cTnT exon 47 19 and chicken cTnT exon 518 (Fig 3⇑, and see below). From these data, we concluded that in human cTnT cDNA the sequence variably included in this position was 30 nt in length.
PCR reactions over the remainder of the coding sequence revealed no length heterogeneity. This differs from the rabbit and the rat, in which variable expression of a 9-nt exon, encoding QAQ and identified as exon 12 in the rat gene, yields QAQ, AQ, or complete exclusion of the exon.19 20 Sequencing 12 products from two fetal and newborn RT-PCR reactions revealed they all contained the 9-nt region, encoding QAQ. In five full-length cDNAs from RT-PCR and the two clones from the adult libraries, the 9-nt exon was always present. Thus, we found no variability in the splicing pattern of this exon in human cTnT cDNA. Although variable expression was not found, its presence in the rabbit and rat suggest that variable expression of this sequence may occur in the human heart, albeit with a low frequency.
In Vitro Expression of the Full-Length cDNAs
The four full-length cDNAs were expressed in vitro. The comigration of the native proteins with the translated products of the cDNAs is illustrated in Fig 4⇓. The full-length cDNAs containing the 30- and 15-nt sequences, the 30-nt sequence alone, the 15-nt sequence alone, and neither of the two sequences yielded cTnT1, cTnT2, cTnT3, and cTnT4, respectively (Figs 2⇑ and 4⇓). cTnT3 is the dominantly expressed isoform in the adult heart3 (Fig 4⇓). cTnT4, which contains neither the 30-nt nor the 15-nt sequence, is a fetal isoform whose expression is increased in the failing human heart (Fig 4⇓). The fetal isoform cTnT1 appears at a similar level with cTnT4 in the fetal heart (Fig 4⇓), whereas cTnT2 appears at a very low level (Fig 4⇓).
Polyclonal antisera, raised against the 10-residue peptide, EEDWREDEDE, reacted with the two largest isoforms in Western blots of immature human, rabbit, and rat myocardium. We were not able to test immunologically for the presence of the five-residue peptide, encoded by the 15-nt exon (Fig 2⇑), because of the apparent lack of antigenicity of this short peptide.20 On the other hand, the cardiac-specific MAb 13-11 recognized all the isoforms in the human, rabbit, and rat myocardium, as the epitope is encoded by a portion of the coding sequence shared across several vertebrate species.
Analysis of the Rat cTnT Gene
Previous analysis of the rat cTnT gene19 provided an exonic pattern that differed from that of the chicken gene by the absence of an exon homologous to chicken exon 4. The chicken sequence encodes a six-residue peptide, EEYVEE, which is similar to five- and six-residue peptides found in the same position in cow, sheep, and rabbit cTnT isoform sequences (Fig 3⇑). Our finding an alternatively spliced 15-nt sequence in human cTnT cDNA that encoded a peptide similar or identical to five- or six-residue peptides found in the same position in cow, sheep, and rabbit cTnT suggested that such an exon may be a common feature of vertebrate cTnTs. Searching of the intronic sequences between exons 4 and 5 of the published rat gene sequence19 revealed the potential for an additional exon that encodes a five-residue peptide, AEAVE. The putative coding sequence was flanked by the canonical 3′ splice donor site (AG) and 5′ splice donor site (GT) sequences26 as well as a polypyrimidine tract and a natural branch-point sequence for lariat formation. Finding all of these sequence features suggests that alternative combinatorial splicing of this novel exon and the previously recognized rat 30-nt coding sequence will yield four rat cTnT isoforms, the two largest containing the 10-residue peptide, consistent with our Western blot analysis of fetal and adult rat myocardial proteins (Fig 4⇑).
The sequences of the four human cTnT isoforms that are differentially expressed in the fetal and normal and failing adult hearts are identified in the present study. The functional significance of the altered cTnT isoform expression and decreased myofibrillar ATPase activity in the failing human heart3 27 can now be considered in context of the sequence differences among the isoforms. These cDNAs and their translational products will be useful in future tests of these functional relations.
We have determined that the four cTnT isoforms observed in human heart are generated by combinatorial alternative splicing of two 5′ exons, a 15- and a 30-nt 5′ sequence. Both exons encode highly acidic peptides; the inclusion of either peptide would add overall negative charge to the cTnT protein. A consensus full-length cDNA, which we obtained from adult heart cDNA libraries, and a full length cDNA, obtained from RT-PCR, contain the five-residue peptide encoded by the 15-nt exon and confirm the sequence of an adult cTnT isoform deduced from previously published sequences.23 24 This isoform, which we have named cTnT3, is the dominant isoform expressed in the adult human heart, on the basis of Western blot analysis. We have confirmed that this 15-nt exon is alternatively spliced; it is excluded from two isoforms (cTnT2 and cTnT4) that are expressed in the fetal heart, with cTnT4 being reexpressed in the failing adult heart. Although Townsend et al23 previously described a 27-nt sequence obtained from an RT-PCR product generated from the 5′ region, our 30-nt sequence is consistent with the length of the alternatively spliced exon found in rabbit, rat, and chicken cDNAs and in the rat and chicken cTnT gene.7 18 19 20 The 30-nt sequence is most likely the actual size, in that it encodes the identical peptide deduced from an alternatively spliced rabbit 30-nt sequence20 and that the two largest human cTnT isoforms that are expressed in the human fetal heart (cTnT1 and cTnT2) are recognized by a polyclonal antiserum raised against the deduced 10-residue peptide.
The human cTnT isoform cDNA sequences, the previously analyzed rabbit and rat cTnT cDNAs,19 20 and the results of reexamining the sequence of the rat cTnT gene demonstrate in the mammal a general pattern of combinatorial alternative splicing of the primary cTnT transcript. Fig 2⇑ illustrates the combinatorial alternative splicing of a 15-nt sequence (in the rabbit, an 18-nt sequence; Fig 3⇑) and a 30-nt sequence in the amino-terminal region. We have not identified in the human cTnT molecule any other regions in which alternative splicing occurs. A 3′ sequence encoding QAQ has been shown in rat and rabbit cDNA to be variably expressed as QAQ, AQ, or not at all.19 20 We searched for this polymorphism in the human cTnT mRNA population and consistently found only the 9-nt exon encoding QAQ. The possibility of a variable expression of QAQ must be considered when human cTnT mutants are sought.
The finding of Thierfelder et al,14 who reported that mutations in the cTnT gene are present in affected members of some families with hypertrophic cardiomyopathy, makes it important to know the sequences that are alternatively spliced as well as allelic differences when screening for further mutations in human cTnT.14 The identification of alternatively spliced sequences will be useful in analyzing genomic DNA to establish gene organization and RNA to search for mutations. If screening approaches based on RNA analysis are used, the sequence variability identified in the present study could lead to results that suggest cTnT mutations. Additionally, the identification of these exons suggests that nonsense mutations in these alternatively expressed sequences could result in truncated translational products and loss of an isoform or loss of isoform-specific function and produce the hypertrophic cardiomyopathy phenotype. In addition, recognizing the presence of these exons allows mutations to be sought in their splice donor or acceptor sites that could yield incorrectly spliced transcripts and protein products.14 28 29
The functional significance of the variable inclusion of the 5- and 10-residue peptides in the various cTnT isoforms is suggested by previous studies. The previous studies of Nassar et al15 and McAuliffe et al17 suggest that the presence of isoforms containing the 10-residue peptide increases myofilament sensitivity to calcium. In the human heart, cTnT2, which contains the alternatively spliced 10-residue peptide, is found in very small amounts throughout development. However, cTnT1, which contains both the 10- and 5-residue peptide, is expressed at a high level in the fetal human heart. This high expression of an isoform containing the 10-residue peptide may be functionally important in the immature myocardium, where the peak cytosolic calcium concentration transient is significantly less than that of the adult.30
The predominantly expressed adult human isoform, cTnT3, contains the 5-residue peptide and lacks the 10-residue peptide, whereas the fetal isoform, cTnT4, which is reexpressed in the presence of heart failure, lacks both peptides (Fig 2⇑).3 A study using a protein assay reconstituted with either of the two bovine cTnT isoforms10 demonstrated that an amino-terminal 5-residue peptide similar in charge and position to that of the human 5-residue peptide altered the sensitivity of ATPase activity to calcium.16 We have found that in human left ventricular myocardium, peak myofibrillar ATPase activity is correlated with cTnT4 expression.3 Altogether, these results are consistent with the disease-associated shift in cTnT isoform expression in the human heart altering myofilament function. Moreover, these data identify specific sequences whose effects on myofilament function should be tested.
Given the role of troponin T in myofibril function, myofibrillogenesis, and hypertrophic cardiomyopathy and the effects of development and disease on human cTnT expression, the data provided by the present study should prove useful in testing how cTnT isoform expression affects hearts of patients of different ages, hearts with different cardiac defects, and hearts exposed to abnormal hemodynamic states. The cDNAs of these human cTnT isoforms will prove useful in searching for cTnT mutations among patients, and the recombinant proteins generated from these cDNAs will prove useful in testing the functional significance of these isoforms.
This study was supported in part by the Gustavus and Louise Pfeiffer Research Foundation and the National Institutes of Health (grants HL-42250 and HL-20749). The authors thank Dr Nils B. Adey for his technical advice on cloning.
- Received October 24, 1994.
- Accepted December 13, 1994.
- © 1995 American Heart Association, Inc.
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Anderson PAW, Malouf NN, Oakeley AE, Pagani ED, Allen PD. Troponin T isoform expression in humans: a comparison among normal and failing adult heart, fetal heart, and adult and fetal skeletal muscle. Circ Res. 1991;69:1226-1233.
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Anderson PAW, Moore GE, Nassar RN. Developmental changes in the expression of rabbit left ventricular troponin T. Circ Res. 1988;63:742-747.
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Sabry MA, Dhoot GK. Identification of and changes in the expression of troponin T isoforms in developing avian and mammalian heart. J Mol Cell Cardiol. 1989;21:85-91.
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Malouf NN, McMahon D, Oakeley AE, Anderson PAW. A cardiac troponin T epitope conserved across phyla. J Biol Chem. 1992;267:9269-9274.
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Jin J-P, Lin JJ-C. Isolation and characterization of cDNA clones encoding embryonic and adult isoforms of rat cardiac troponin T. J Biol Chem. 1989;264:14471-14477.
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Westfall MV, Reiser PJ, Schiaffino S, Solaro RJ. Developmental isoform switching of TnT and TnI in rat ventricle and atria. Biophys J. 1992;61:A20. Abstract.
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Toyota N, Shimada Y, Bader D. Molecular cloning and expression of chicken cardiac troponin C. Circ Res. 1989;65:1241-1246.
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Nassar R, Malouf NN, Kelly MB, Oakeley AE, Anderson PAW. Force–pCa relation and troponin T isoforms of rabbit myocardium. Circ Res. 1991;69:1470-1475.
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Tobacman LS, Lee R. Isolation and functional comparison of bovine cardiac troponin T isoforms. J Biol Chem. 1987;262:4059-4064.
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Cooper TA, Ordahl CP. A single cardiac troponin T gene generates embryonic and adult isoforms via developmentally regulated alternate splicing. J Biol Chem. 1985;260:11140-11148.
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- Molecular Basis of Human Cardiac Troponin T Isoforms Expressed in the Developing, Adult, and Failing HeartPage A. W. Anderson, Ann Greig, Trisha M. Mark, Nadia N. Malouf, Annette E. Oakeley, Ross M. Ungerleider, Paul D. Allen and Brian K. KayCirculation Research. 1995;76:681-686, originally published April 1, 1995https://doi.org/10.1161/01.RES.76.4.681
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- Molecular Basis of Human Cardiac Troponin T Isoforms Expressed in the Developing, Adult, and Failing HeartPage A. W. Anderson, Ann Greig, Trisha M. Mark, Nadia N. Malouf, Annette E. Oakeley, Ross M. Ungerleider, Paul D. Allen and Brian K. KayCirculation Research. 1995;76:681-686, originally published April 1, 1995https://doi.org/10.1161/01.RES.76.4.681Permalink:


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Welcome to Rescue Me! Rescue Me has two parts. The first part involves featuring a local animal welfare organization and what they are doing for the community, including adoptable animals. To learn more about this month's featured agency, please click here.
The second part is a weekly video segment featuring adoptable dogs from Gateway Pet Guardians. Each week, I feature a new dog up for adoption and in the video, you will see how they interact with others, what their personality is like, and other important information about the featured dog.
This week, I would like to introduce you to Ron Ron! He is just a fun ball of energy that wants to entertain you all day!
Related Link: Have you met Jackie? Learn more about her story here!
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Well, i'm off again to my very first ship I ever worked, the elation. (which actually was only 2 months ago) Hope to see everyone there, i believe we have a new cruise director, and if any "frequent boaters" are back on board, please come say hi.
I'm leaving monday for progresso and cozumel again. Love diving there with my fabulous divemaster Alejandro Moldenado (Alex) from Cozumelbesdives http://www.cozumelbestdive.com/eng/about_us.cfm thanks so much for the fun!
Here i am all geared up!
And here are a couple of hitchhikers in the middle of the ocean, how they got here, i have no idea….but boy, are their wings tired!!!! Check please, try the veal, we'll be here all night!!
and a few of my new freinds… dead or alive!


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Before you know it, 2014 Summer will reach its final moments. You have been to so many different places, doing many great things, one after the other. But you probably failed to spend a night eating great food and dancing to great spanish songs. Nevertheless, the opportunity to have one of those epic night has just arrived. Go ahead and pay a visit to one of the top five Latin clubs in New York City. Have an amazing night at the magnificent Iguana restaurant. If you love to dance and have fun, than the Iguana restaurant is the right place for you.
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From The Controller To The Big Screen: What It Takes To Make A Great Video Game Movie
To date, there are only 29 theatrically released American films based on video game properties, yet the highest aggregate score for any of them has been a 58/100 (according to Metacritic.com). With so many quality original stories available from the medium, why has there not been a single one that is generally accepted to be a high-quality movie?
As an intense lover of all things video games, this fact is especially hard for me to swallow. I've always wanted non-gamers to understand just how interesting the stories in video games can be. Since you would be hard-pressed to find anyone who doesn't have at least a minor interest watching an occasional movie, film has always seemed like the easiest method to get that point across. Yet every time I walk into a theater hopeful that this will be the one that will finally stands out as an excellent adaptation of one of my beloved franchises, I walk out of that same theater just as disappointed.
That's not to say that I haven't enjoyed any of these movies. On the contrary, I've had a lot of fun seeing familiar characters and settings brought to life on the silver screen. But I can count on one hand a number of titles that I would legitimately recommend to anyone. There are plenty of opinions out there as to why we haven't had what many would consider an excellent video game movie, but I wanted to take a shot at it from the perspective of someone whose first love was gaming instead of movies.
Video games can have mature stories
Despite the fact that the average age of gamers is 35 years old and that only 25% of those who play video games are under the age of 18, so many people look at this hobby as an activity for children. To many people, it's nothing more than a fancy toy and nothing more. This stigma causes a lot of people to shy away from these stories, probably because they're expecting nothing deeper than a mustachioed plumber saving a princess from an evil turtle king. But there's so much more available when it comes to original plots.
Unfortunately, the first attempts at bringing video game characters to life took place when story wasn't the forefront of importance in video games. When the infamous "Super Mario Bros." hit the screen in 1993, video games were still somewhat gaining their footing. Games were primarily about mechanics as people we still getting used to interacting with their media instead of merely watching it. This trend continued with movies like "Mortal Kombat", "Double Dragon", and "Street Fighter", all of which were games with very little in terms of narrative. Times have changed and games much more intricate storylines than ever before, but it seems like only those who play them know this. I'm of the belief that people have to get over this mindset and be a bit more open to stories that come from a place they are not familiar.
Not only that, but filmmakers need to do a better job of picking better source material. It seems to me that the majority of the intellectual properties adapted from games to film are ones that the suits assume will appeal to a broad audience. People crave something fresh and exciting, not always just another mindless action flick with eye candy and a couple recognizable characters. Instead of choosing "Bloodrayne", "Dead or Alive", or "Doom", why not tackle the underwater utopia of "Bioshock" or post-apocalyptic wasteland of "Fallout"? Those are two examples of an incredible list of games with bold, interesting storylines that would translate very well to film.
Film adaptations need more fan service
I completely understand that movies and video games are not the same thing, nor should they look identical to each other. But in order to win over an audience of gamers (an audience that is growing larger by the day), there needs to be more familiarity to the original source material. Earlier I mentioned "Super Mario Bros.", a film that pretty much only had character names in common with the original game. It's considered to be one of the most bizarre realizations of an imaginary world. The closest I've seen a movie get to what the game looked like was "Silent Hill", which had a few almost exact camera angles from that were very recognizable from the game. But it wasn't enough. The film eventually strayed away from its roots and focused on other things than what made the game series so good.
The easy comparison here is comic book movies. Do they follow the comics exactly to the letter? No. Are the characters and situations instantly recognized by those who read them and loved them as kids? Definitely. Video games need the same treatment to win those fans, otherwise the "genre" will never be looked at anything other than a joke.
Fans of video games need to get to the theater
Although it's not based on a video game property, a movie called "Scott Pilgrim vs. the World" came out in 2010 and gave me hope for a spark for video game movies. While it wasn't the best movie I saw that year, it was one of the most fun and gave me a ton of nostalgia. Sadly, my theater was quite empty on opening weekend and the film fell over $10 million short of its budget. How can we expect studio executives to green light more films of this nature if the audience for whom the movie was made doesn't show up to see it?
It begs the question: "Where are the fans?" How can we complain about these movies not getting any better, when we're not willing to contribute to the ones that are just "OK"? Also, why are the only successful adaptations ones that barely resemble the games on which they are based? "Prince of Persia" arguably had nothing to do with the game other than its setting, yet its one of only three movies based on a video game to break the $250 million mark.
This isn't to say that I'm not hopeful for the future of video game movies. This Christmas, we're getting "Assassin's Creed", a game franchise with a very intriguing storyline that actually ignited my interest in history. Fans of archeology should also be excited about the film adaptation of "Uncharted", which is due to come out in 2017. But if these stories are ever going to get heard by a broader audience, something has to change. I only hope that change is already in motion, and that this summer will be the start of something great for gamers.
Because we deserve it.


text: here is a quote from the Times on line (link below to entire article) Note those provisos, because in time all classics become predictable and have to be rethought. Even the Hermès Birkin is becoming ubiquitous to the point where fastidious status-hunters are putting theirs in storage in favour of Bottega Venetas equally luxurious, but more anonymous, bags. http://women.timesonline.co.uk/tol/life_and_style/women/fashion/article2900969.ece


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Heidi is approved to conduct lessons through Wyzant Online. Wyzant Online allows students and tutors to work remotely via video, audio, and collaborative whiteboard tools. For more information about how online tutoring works, check out Wyzant Online.
If you're interested in online lessons, message Heidi to get started.
Send a message explaining your needs and Heidi will reply soon.
Response time: 2 hours
University of Liverpool (Chemistry)
University of Bath (PhD)
Arizona State University (Other)
Hello, my name is Heidi. I have taught as adjunct in the Community College. I have taught various chemistry courses including general chemistry and organic chemistry.
I hold the CRLA (College Reading and learning association) regular tutoring certificate.
I graduated with a first class bachelor's degree in Chemistry with honors from the University of Liverpool, England. During my degree I spent a year working in industry for Zeneca. During this year I gained practical experience in Ion exchange chemistry.
After graduating I gained another years experience in industry concentrating on the field of combinatorial chemistry. I became a mentor for junior team members.
I then returned to my studies and was awarded a PhD in medicinal chemistry from the University of Bath in England. My field of expertise was sugar chemistry. During my 4-year course, I tutored many undergraduates in lab work.
I have post doctoral research experience in Cancer research from Arizona State University.
I have chosen to exclusively tutor chemistry as I have a lot of experience in the subject.
My strength lies in the ability to explain organic chemistry into simple language. This includes first and second semester college organic chemistry. I studied inorganic, physical and organic chemistry for 6 semesters in England. I specialized in organic chemistry in my final year.
I am also confident in explaining general chemistry both at high school and college level. Example of topics that I cover are stoichiometry, gas laws, thermodynamics, chemical bonding and VSPER theory, acid/base chemistry, kinetics, equilibrium etc.
My style changes with each individual student. As I was educated in England, a very often have different methods of working out calculations which students find much more simple.
Many students get very confused by stoichiometry. This is an extremely important concept and if a student is confused by this topic they will continue to struggle with chemistry. Most of my students will understand stoichiometry within their first lesson with me.
I am willing to answer questions via email in between regular sessions. Hello, my name is Heidi. I have taught as adjunct in the Community College. I have taught various chemistry courses including general chemistry and organic chemistry.
I hold the CRLA (College Reading and learning association) regular tutoring certificate.
I graduated with a first class bachelor's degree in Chemistry with
Rates quoted are daytime rates at Westbank Community library or Starbucks (Beecave Road). Evening and weekend rates are more. Group of 2 or more add $20 per person, up to 3 people.
Heidi is approved to conduct lessons through Wyzant Online. Wyzant Online allows students and tutors to work remotely via video, audio, and collaborative whiteboard tools. For more information about how online tutoring works, check out Wyzant Online.
If you're interested in online lessons, message Heidi to get started.
Heidi is an excellent tutor for organic chemistry. She came on time. I find it useful to me that she suggests using a flash card to memorize the multiple reactions. She answered all my questions and summarized the concept for me in an easy way that I can understand. Thanks for your time and for helping me understand the materials.
We talked a lot about what our tutoring sessions would include. She named a few topics in OChem that I might want to review before we started, just to make sure things go smoothly. She was very helpful and I look forward to working with her.
Heidi is a extremely knowledgeable tutor - she assisted me in Chemistry II, Organic Chemistry I and Organic Chemistry II. She is very patient and explains in a clear and concise manner.
We've had great luck finding Mickie B, our Honors Chemistry Tutor. We scan our child's study guide, and Mickie reviews it prior to the session. Our daughter really appreciates Mickie, and gets along great with her.
The one time we could not schedule with Mickie, we found a terrific substitute, literally at the last minute. That was Heidi. Heidi emailed me within the hour with a great location to meet, and she accommodated our last minute need for a tutor. I am grateful for this service. We would definitely contact Heidi again, and appreciate her skill at explaining the topic matter.
She started working with Heidi part way through Organic Chemistry. My daughter was sure she was going to fail, but I said take the whole class anyway, so if you have to take it again it's familiar to you. She passed! She was one of the only students who had not taken chemistry in high school, so she was struggling. She told me that when Heidi explained things she suddenly understood them. She also encountered some other students who had been tutored by Heidi and their experiences were also very good. Heidi was very flexible and was able to see my daughter right away when we contacted her for help. (It was a chemistry crisis!) She was also very flexible about working with my daughter's schedule and meeting her near campus. I most highly recommend Heidi R as a tutor! This was a 100% positive experience for us!
Heidi is the only reason I was able to pass my 8 week organic chemistry course. She is patient and has an extremely flexible schedule. I was able to meet with her weekly. She walked me through my homework problems and answered all of my questions in great detail. I would recommend her to anyone who is struggling with organic chemistry.
Heidi contacted me within minutes of my initial request. We were lucky enough to be able to schedule a hour within a couple of days, just in time for a Chemistry test. She is quite knowledgeable and was able to answer all my daughter's questions quickly and clearly. My daughter appreciated her patience. I appreciated her flexibility. The results are in the performance: my daughter reported the test was easy thanks to Heidi's coaching.
Heidi was a great tutor for my daughter in Chemistry. She was always accommodating. Heidi's schedule was flexible to work with my daughter's schedule. Heidi had a great way of explaining the material so my daughter was able to achieve success.
Chemistry is not my strong suit and I was struggling when I got in contact with Heidi. She was able to simplify and clarify the structures and mechanisms in a way that made sense to me. She is the only reason I was able to pass this course and I greatly appreciate her availability and knowledge.
Heidi is really awesome. She's so patient and was very willing to work with my schedule. She was also really easy to get into contact with via email or by phone. She definitely helped me grasp concepts in a way I could never have accomplished on my own. Thank you Heidi!!
Heidi is very knowledgeable and came prepared to work on the Chemistry topics my son needed to focus on. She was patient when explaining concepts, even if she had explained the same concepts the session before. She left homework expectations after each session and also left the answer keys with me so I could help in between. She took the time to provide session summaries for me so I could help ensure my son was getting everything he could from the tutoring time. I'd recommend Heidi as a great tutor for focused and driven students who want to exceed!
I would meet with Heidi about one to two times a week and it was always a great learning experience. I was determined to work hard and came prepared with questions for her, which she gladly helped me through and worked with me on. I ended up getting a good grade in the class because of her help! Definitely getting tutored by Heidi next semester and would recommend her to ANYONE wanting to succeed! Thanks Heidi!
I really don't like or understand chemistry. I had to take the first part of Ochem twice and wouldn't have passed it the second time without the help from Heidi! I only wish I had found her earlier, maybe I wouldn't have had to take the class again! I felt like I didn't know anything and after only two sessions with Heidi I was ready to take my ACS final! I highly recommend her to anyone looking for help in Organic Chemistry!
Heidi is an amazing tutor. She is a General Chemistry and Organic Chemistry Whiz. She genuinely cares about her students and really tests your knowledge. She also helps simulate testing conditions so you really know where you stand for any type of major test. I would highly recommend her for all your chemistry needs.
Heidi is a great tutor. She will take the time to work with you through the problems that you need help with. She will also provide worksheets of her own to help you get more practice. Heidi has worked with my schedule very easily, and has found times to help me that worked for both of us. She is very patient and understanding of your situation. I found that my test grades improved quite a bit after working with her. Definitely worth the price!
Heidi was amazing! She was able to help me pass Organic Chemistry. If it wasn't for her, I would have had to repeat the class. She was able to reword the information into easier terms which allowed me to understand. She was always available for my schedule and would return all my emails and phone call quickly. If you want to pass the first time, I would choose Heidi.
Heidi is extremely well versed in Chemistry. She seems to be best suited for students that find it easy to work following the text book in a structured way and willing to do homework to keep up with the instructions. Overall I think she is a very good tutor. Since the tutoring is in a library versus home, it can be inconvenient for folks not having a vehicle.
I always like to meet my students in a public place such as a library for the first lesson or so.
Heidi made her tutoring style match my learning style, which led to a very effective tutoring session. She explains concepts thoroughly and clearly, doesn't move too fast, and made sure I worked out problems on my own.
I've been going to Heidi for the past year with my O-chem and she's always very helpful. Very flexible schedule and explains things very well. Great tutor!
Heidi is a very helpful, accommodating and knowledgeable tutor. My only regret is that I didn't get Heidi to tutor me earlier. It would have saved me a lot of frustration and I am sure I would have gotten an A instead of a B. Nevertheless, Heidi helped me bring my near failing grade up to a B...that I am really proud of. Thanks Heidi.
She knows her material hands down and her schedule is flexible. I would not of trusted anyone else to tutor me in this subject, because I feel that no one else is as qualified as she is! She breaks down the mechanisms and reactions so it is more understandable. HIGHLY recommend!
Heidi is a very knowledgeable and patient tutor. My daughter is in high school and had a very hard time with chemistry, and Heidi was able to help her understand better than her teacher. My daughter's grades improved as a result. Heidi is obviously a wonderful tutor.
Heidi has a true passion for the subjects she tutors which translates into superior quality learning for the student. She has the ability to work at the student's pace and explain concepts in ways the student can understand. She is amazing and truly a gift! Thanks Heidi! :)
Great tutor! Heidi has been very helpful to me and she is very patient with me.
Very Good Tutor
Heidi is a very patient tutor, she explains things very clearly, she writes down good examples. After her tutorial I got 98% in my homework.
Heidi is a great tutor! I appreciate the time she spends preparing for our sessions and she is extremely responsive. Heidi is patient and always willing to help explain concepts several times if needed.
A very special thanks to Heidi!!
Approved subjects are in bold.
In most cases, tutors gain approval in a subject by passing a proficiency exam. For some subject areas, like music and art, tutors submit written requests to demonstrate their proficiency to potential students. If a tutor is interested but not yet approved in a subject, the subject will appear in non-bold font. Tutors need to be approved in a subject prior to beginning lessons.
Chemistry-I teach all levels of general chemistry and organic chemistry. As my degree is in Chemistry from England I studied chemistry for 6 semesters. Having problems with the calculations, I can make them easier.
I have been teaching the MCAT chemistry section for the last two years. I have helped a number of students achieve their goal in this area.
I believe I can help people increase their scores on the chemistry sections of the MCAT. There is a lot of information to remember when taking the MCAT from the 4 semesters of chemistry. There is also new information to learn such as doing the math without the calculator.
I have studied organic chemistry as an undergraduate for 6 semesters in an English University. In England particular attention is given for understanding mechanisms. I also did a specific course for retrosynthetic analysis (this is where you are given a product and asked what starting materials were required).
In addition my PhD is in Medicinal organic chemistry. I have a lot of synthetic experience including post doctoral lab work.
Experienced tutor and organic synthetic chemist (adjunct professor)


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I first saw these when I was at the Biltmore Estate gift shop in Asheville, NC. I fell in love with them immediately and bought one for the studio. Well, so many clients loved the one we had that I wanted to get another. Turns out they weren't so easy to find. After some online sleuthing I found the company that distributes them in CA. I contacted them and was able to secure some for resale. The Illuminated Silver Dollar Branch is perfect in centerpieces for weddings, special events, or holiday parties. Electric lighted branch will even make a statement if displayed alone. Bendable metal twigs have clear incandescent bulbs with synthetic, white silver dollar florals. The 20"H has 1 stem at the base; 40"H has 3 stems at the base. Each stem has multiple twigs. Simply unplug the electric branch when not in use. UL listed. Indoor only. Measurements are approximate.
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Obituary: Statler, Eugene Donald
Sun., July 14, 2013
He is survived by his wife of 54 years Lois Statler a sister, Virgie Mays and 9 children, Donna Wood, Kate Stang, Joyce Nelson, Nina Cornwell, Harvey Bowers, Edward Bowers, Janelle Statler Larson, Duane Statler and Kenneth Statler.
He was a grandfather to 16 and a Great-Grandfather to 25.
He was a millwright at ADM/Centennial Mills and a Sergeant in the US Army.
An avid CB and HAM radio operator a love he shared with his son.
After retiring he spent several years as a snowbird traveling to and from Arizona with his wife.
He loved to fish and camp.
Recent years were spent at home with family and friends.
He was ill but never complained.
We Love You Daddy…A Graveside Service will be held on Tuesday, July 16 at 10:00 am at Greenwood Memorial Terrace.
A Celebration of Gene's life will follow, at noon on the same day at the family home.


text: "That's what it really is if you look hard. A forest of large stumps." Farmer Greg stood up against one of them so I could take a photo for size comparison. I looked up at the sky through the branches of trees that looked pretty big all the same and imagined a landscape of larger trees with dense understory of moss and ferns. I must have been an amazing thing to see.


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Quick Details
- Usage: Garment, clothes
- Clothing Type: Shirts, kids clothes, suit, coat,
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Manufacturers Clothing Pant Skirt Hangers for Clothing Shop
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text:
Sam McKegney
Associate Professor
PhD Queen's, CV/Bio (PDF 261 KB), Website
613-533-6000, ext. 74388
Office: Watson Hall, Room 435
Office Hours: Tues. 1:00–2:30 pm, or by appointment
2017-18 Courses
- ENGL 290 003/3.0 Seminar in Literary Interpretation – Eden Robinson's Monkey Beach
- ENGL 489 001-3/3.0 Group III: Special Topics II – Hockey[,] Literature and Canadian Myths of Nation
Research Interests
- Indigenous literatures
- contemporary Canadian literature (as well as its precursors)
- masculinity theory
- Indigenous governance and its pursuit through art
- carceral composition
- multiculturalism as an ideal and in practice
- hockey culture
- literary activism
Recent Publications
Masculindians: Conversations about Indigenous Manhood. Winnipeg: University of Manitoba Press, February 2014.
"'pain, pleasure, shame. Shame'—Masculine Embodiment, Kinship, and Indigenous Reterritorialization." Canadian Literature 216, Spring 2014. 12-33. (Winner of the Canadian Literature Award for Best Essay 2014.)
"Writer-Reader Reciprocity and the Pursuit of Alliance in Indigenous Poetry." Sounding Out: Indigenous Poetics. Eds. Neal McLeod and Natasha Beeds. Waterloo: Wilfrid Laurier University Press, March 2014. (Winner of the Gabrielle Roy Award for best work of Canadian Literary Criticism.)
"Strong Men Stories: A Roundtable on Indigenous Masculinities with Richard Van Camp, Warren Cariou, Gregory Scofield, and Daniel Heath Justice." In Indigenous Masculinities Reader. Eds. Kimberly Anderson, Robert Innes, and Jonathan Swift. Winnipeg: University of Manitoba Press, 2015.
Current Supervisions
Trevor Phillips, Catherine André, Andrew Law, Sarah Kent, Jamieson Ryan (about students)


text:
Princeless: Encore Edition #3 (Preview)
in Comic Previews Comment
- Story by
- Jeremy Whitley
- Art by
- M. Goodwin
- Cover by
- M. Goodwin
- Publisher
- Action Lab Comics
Read for yourself the issue that was nominated for the Eisner for Best Single Issue! Adrienne has escaped her father with only the armor on her back…and that doesn't even fit. Now she must face the most trying moment in a young heroines life…finding real armor for a girl! Will she ever escape the sea of leather corsets and chain mail bikinis?


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More information about the markets that we regulate.
Business retail market
The largest competitive water retail market in the world is open for business, bringing the biggest change to the water sector since privatisation.
New Appointments and Variations (NAVs)
The NAV markets involves one company replacing another as the appointee for a specific geographic area.
Connections market
This market includes all new connections to the water and/or sewerage network for both household and non-household purposes.
Bioresources market
Changing how the sector transports, treats, recycles and disposes of wastewater sludge.
Water trading
A market where water companies purchase water from elsewhere rather than developing its own resources.


text: Rods are photoreceptor cells in the retina manage our scoptic vision. They help our eyes adjust to darkness after 30 minutes. Rods see black, white and shades of gray. They also help us identify the shape of an object. Because I don't have information on the type of rods you are referring to, I help my answer helps you with what are rods. For more information see here:


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Report Copyright Infringement View in OSM UK View in OSM NZ
Make a Japanese Fan, learn about the traditions behind the Japanese fans
Lolly sticks
Decorate paper, cut into strip and fold, stick to lolly sticks (see attached photo)
Info about traditional Japanese fans:
Japanese fans are made of paper on a bamboo frame, usually with a design painted on them.
The number of strips of wood differed according to the person's rank.
They are used today by Shinto priests in formal costume and in the formal costume of the Japanese court (they can be seen used by the Emperor and Empress during coronation and marriage) and are brightly painted with long tassels.
Simple Japanese paper fans are sometimes known as harisen
Even today, Geisha of all types (but maiko most often) use folding fans in their fan dances as well.
The fan is primarily used for fanning oneself in hot weather.
Rainbow, Brownie, Guide and Senior Section leaders should visit Online Guide Manager.
© 2011, 2018 Online Youth Manager Ltd.


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When I started to write this article on cutting the cord and getting all my TV via the Internet instead of cable or satellite I quickly realized that given the technology convergence, it wasn't at all clear what I meant by "TV".
It used to be very easy, TV was the device in your living room that received broadcast radio waves through an antenna either on top of the unit as "rabbit ears" or a bigger array on your roof. Can you imagine having one of those today? But with the plethora of video content on the web and the devices by which we can consume that content, the lines have been blurred to the extent of confusion.
So let me share my confusion with you. Is TV a device, or is it content, or is it delivery? What does it mean these days to say "I'm going to watch TV."?
Can I "watch TV" on my tablet? If your answer is yes, then "TV" isn't really a device.
Can go to the store and buy a TV? I think we all know the answer to that one, so that means TV is a device… right? OK, that's a tricky one. Let's ignore it for now.
If I "watch TV" on my tablet then clearly I'm referring to content, but is it only content from broadcast and cable channel providers meaning something you could watch on your device called a TV. This would imply that watching on demand video's such as Netflix would not be "watching TV"? And if I am watching Netflix through my Roku or tablet streamed onto my device called a TV, am I watching TV?
Now, back to the concept of defining TV as being from broadcast and cable channel providers… what about web only content from a cable channel? I can watch Food Network's "Chopped" on their channel, but if I want to watch "Chopped After Hours" I can only stream it from their website. So if I watch both of those on my tablet back to back, did I go from "watching TV" to not "watching TV"?
What if I'm watching a "TV show" such as NBC's "Friday Night Lights" on Netflix… on my phone? Is that "watching TV"?
This can go on forever. Basically what I'm saying is that the term "TV" is meaningless today. It's all about consuming video content. And for me I think it is time to cut the cord (specifically dump the satellite dish – and associated monthly bill) and join the throng moving to web only. And why not? Why am I paying $100 per month for 600 channels of which we watch two or three? And if it weren't for DVR so that I can do it on my schedule and skip commercials, I wouldn't even watch that much. But with the web, it's all on demand.
The cable and satellite companies have to be scrambling to figure out how to remain relevant. The only thing they'll have left to offer is bandwidth onto the Internet, and the satellite companies have always struggled with providing that as a service. Oh well, enough for today. I think I'll go consume some video content.


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Actor Prabhas underwent noticeable physical transformation for portraying the two generations of the fictional character Baahubali, father Amarendra and son Mahendra, in SS Rajamouli's series of epic filmsBaahubali: The Beginning and Baahubali: The Conclusion.
Prabhas has been trained by professional bodybuilder Lakshman Reddy, who hails from Hyderabad and is the winner of Mr World 2010, reported mid-day.
Mr Reddy reveals Prabhas weighed some 100 kilos for playing the part of Amarendra Baahubali but his role as Shivudu, as seen in Baahubali 1, required him to sport a toned body.
"As Baahubali, Prabhas had to build a lot of muscle and for the son's character, Shivudu, he had to look lean. His physique fluctuated for more than four years; that was difficult.
Prabhas weighed almost 100 kgs with his body fat percentage staying in the range of 9-10. For the younger character, he had to build a toned body with minimal muscles," Mr Reddy told mid-day.
Prabhas' training regime, shooting schedule and diet for Baahubali 1 and its sequel were an absolute contrast, revealed Mr Reddy.
"He would have six meals with egg whites, chicken, nuts, almonds, fish and vegetables thrown in. For Baahubali prep, he had eight meals a day, carb-heavy with cheese and mutton. In the evenings, we had weight training sessions, which included dead lifts, squats, bench press and other rigorous exercises," mid-day quoted Mr Reddy as saying.
Prabhas, who loves biryani and often craved for junk food, was watched very, very closely.
"He loves biryani, so he was allowed that. At times, he'd ask for junk food. I was strict, but understood his cravings. I was continuously monitoring his food intake and workouts," Mr Reddy told mid-day.
"He would shoot for hard-core stunts and by the end of it, be tired, but never missed workout. There were days when we would start exercising at midnight. His dedication was amazing," Mr Reddy mentioned in his interview with mid-day.
Baahubali: The Conclusion will release in Tamil, Telugu and Hindi on April 28.


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All out, sisters
Despite Rachel Sylvester's assertions of a macho culture, the three education trade unions that have voted to strike are all led by women
Sir, While writing about the macho "brothers" running the public sector workers' trade unions, Rachel Sylvester ("Sound and fury will get the unions nothing", Opinion, June 21) seems to have forgotten that the general secretaries of the three largest teacher trade unions: Margaret Bousted, ATL; Christine Blower, NUT (both unions will be taking strike action on June 30) and Chris Keates, NASUWT (the largest teacher trade union in the UK, whose members will not be striking on June 30) are in fact all women.
Mick Robinson
Willaston, Merseyside
Sir, Rachel Sylvester sees hard men playing hard ball on behalf of the workers. The three education trade unions that have voted to strike are all led by women. When women constitute up to three quarters of…


text: Thanx to all who sent advice. It was the cable, which is actually solid. It is apparently called the Bowden Cable(sp?) and is common to many models. It broke about 2" from where is connects to the interior handle. I have never had the door panel off so it was a learning process but it was not too tough of a fix. Dealer charged a little over $20, which was surprising. My biggest issue was the previous owners wiring setup for the aftermarket speakers. All together and working fine. Thanx again. andy


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- Worldwide Rank #204,499,496
- Follower/Following Ratio 0.13
- Daily New Followers 0
Pequeña Lulu Twitter Stats
@0101_clrock - Tracking Pequeña Lulu Twitter profile since February 21, 2013
Viajera y aventurera
Pequeña Lulu's story
Pequeña Lulu, also known as @0101_clrock has a novice's presence on Twitter and is ranked by us in the 20% percentile for account strength. Active on Twitter since January 2013, Pequeña Lulu made it to having a respectable 8 Twitter followers and to being ranked 204,499,496 for number of followers among all Twitter users.
The plot thickens when considering Pequeña Lulu's follower-to-following ratio, which is 0.13. Which is not surprising given this account's audience size.
From a tweeting perspective, @0101_clrock is a casual user, with an average of 0 tweet(s) per day in the past 30 days. That's pretty consistent with a total of 124 since @0101_clrock joined Twitter.
This account is tracked by us for quite some time now, actually since February 2013. As of January 17 2018 we track 285350849 Twitter accounts, so feel free to search for other accounts you're interested in and reveal their Twitter story!
Pequeña Lulu Tweets
@0101_clrock has posted 0 tweets in the last 8 days, which translates to an average of 0 tweets per day.
Pequeña Lulu Twitter Followers
@0101_clrock has 8 followers on Twitter.
This account is #204,499,496 in the worldwide rank of the most popular Twitter users.
Pequeña Lulu Following on Twitter
@0101_clrock is following 60 Twitter accounts.
Last month this account followed 44 users.
Pequeña Lulu Predictions & Milestones
@0101_clrock will hit 8 followers in the next 3 months, and 8 in one year.
You are on Pequeña Lulu's Twitter stats page
We track these Twitter stats since February 21, 2013 . You can see how many followers Pequeña Lulu lost or gained and what the prediction is for tomorrow or the next 15 days, together with all kinds of other stats like rank compared to all Twitter users, tweets etc.
Why don't you give it a try and compare Pequeña Lulu to yourself ? Or if you are Pequeña Lulu get even more feature rich & up-to-date stats with our premium Twitter Stats.
Twitter Counter is the #1 Twitter stats site powered by Twitter. It all started in June 12, 2008 and we grew rather rapidly because of our Twitter Counter badge. The button is a nice small image that shows your visitors how many followers you have on Twitter. Within a year the button was on many many blogs around the world and we displayed more than 100 million buttons in one month. We track follower stats for over 285.350.849 users now.


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Dorchester Towers Convertible Two Bedroom #1204 For Sale | 155 West 68th Street #1204 New York NY 10023 For Sale
(THIS IS NOW SOLD and CLOSED)
Highly desirable and Rarely Available Spacious convertible Two Bedrooms and One Bathroom is now on market at the Dorchester Towers Condominium.
Dorchester unit #1204 is a corner unit with South and West Exposures brings in lots of sunlight from this 12th Floor unit.
This Dorchester corner unit has a windowed Bathroom, ample Closet Space (total of 6 closets) Throughout. There is a dining alcove ready to be converted to a Second Bedroom.
155 West 68th Street, the Dorchester Towers Condominium Street is one of the most well established Lincoln Center Condominium Buildings. Dorchester Towers Condominium has 24 hours doorman and concierge, a convenient circular driveway, laundry on every floor, roof top deck, bike and personal storage, on-site management and live in superintendent, pet and investor friendly.
(THIS IS NOW SOLD and CLOSED)
If you are a Dorchester Tower condo owner and would like to know the comparable sales in the building, we are happy to discuss all in details with you. Call us today at (917)837-8869 or email us email@example.com and firstname.lastname@example.com , we will be sure to respond promptly.


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"7 Reasons to Submit Your Manuscript! Pub Sub" by Joan Y. Edwards
- It leads you 100 steps closer to publication and has 100% more successful track record for publication than not submitting.
It gives you a wonderful reason to celebrate by going out to eat supper, call a friend you haven't talked with in ages, buy a new craft book on writing, or buy a best-selling book in the genre you write.
It's one step closer to meeting the world's need to hear your voice. The world needs to hear and read the voice of every writer. Do your part to have your voice heard, submit your manuscript.
It's a step closer to receiving a return of money for all your hard work. Never pay a publisher to publish your story. Traditional publishers never charge you money. They pay you a royalty from sales. If you choose to self-publish your work, scout around and find a good deal. It does not cost an arm and a leg to self-publish today when you use print-on-demand. Submit your manuscript to at least 10 publishers/agents before you self-publish.
It leaves you with a great feeling of satisfaction and well-being no other experience can give you. The feeling of knowing you are a professional writer because you submitted your work.
It adds excitement to your world. Now you have the anticipation with wondering what the publisher or agent's response will be. Be ready for either answer, but keep saying the answer is YES.
It feels good to finally reach a goal and submit your work. It feels good all the way from the top of your head down to the soles of your feet.
So drop your excuses. Click on the Pub Subber links below for steps to get your manuscript ready for submission. They are also at the top of my blog. Take days, weeks, or months but, get the necessary critiques, final proofs, queries, cover letters, proposals, or other documents ready. Submit your manuscript. The links have detailed steps. I listed a few of the main parts to help you visualize the process.
Week 1 Send manuscript off for final critique before submission. Choose publisher or agent. Print Guidelines.
Week 2 Write pitch, query, cover letter, proposal, etc. to make a good impression.
Week 3 Proof read everything. Submit this week.
Week 4 Celebrate life. Write another story.
Good luck in your publication. Believe in you and your writing.
Celebrate you.
Never Give Up
Joan Y. Edwards
Copyright © 2014 Joan Y. Edwards
Wanted: 300 Subscribers
Please subscribe to Joan's Never Give Up blog by email from the left-hand column.
You'll receive new blog posts filled with inspiration and information in your inbox as soon as she uploads them.
Never Give Up image
12 Affirmations for Writers.
Filed under: Pub Sub, Writing | Tagged: a wonderful reason to celebrate, adds excitement, closer to being paid money, closer to the world hearing your voice, feeling of satisfaction, feels good, leads closer to publication, Pub Sub, submission |


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From The Associated Press
Obama: No new gun legislation
WASHINGTON — Even as the issue of guns shifts to the forefront of the presidential campaign, the White House and the Senate's top Democrat made it clear Thursday that new gun legislation will not be on the political agenda this year. Instead, President Barack Obama intends to focus on other ways to combat gun violence — a position not unlike that of his rival, Mitt Romney.
The audition for the Missoula Children's Theatre production of Beauty Lou and the Country Beast will be held at 10 a.m. on Aug. 6 at Ilfeld Auditorium on the New Mexico Highlands University campus.
• Seventh Annual Rough Rider Motorcycle Rally, on Friday, July 27 to Sunday, July 29. For a schedule of events see page C8 in today's Optic.
• Vado de Juan Paiz Acequia Association meeting, 1 to 4 p.m. on Sunday, July 29 at the old fire department in Anton Chico, N.M. Fore more information, contact Lisa Gallegos at 429-2079.
Submit your calendar items and notices to firstname.lastname@example.com
• Las Vegas Juvenile Justice Continuum Board meets at 3:30 p.m. on the 4th Thursday of the month at the CYFD office on Ridgerunner Road. The meeting is open to the public. For more information, call Yolanda at 617-0483 or Teresa at 425-3543.
• San Miguel County Family and Community Health Council meets at 10 a.m. on the 4th Tuesday of the month at the Public Health Office on Gallegos Road. The meeting is open to the public. For more information, call Yolanda at 617-0483.
THUMBS UP! UNDER BUDGET. Good news for Las Vegas and its City Council — Finance Director Pamela Marrujo told councilors last week that the city ended its fiscal year in the black. General fund expenses alone came in about $800,000 under budget, while general fund revenues were $351,000 over budget. That's the kind of year-end report we like to see.
In June 1926, exactly three years after his celebrated trial for criminal libel, Carl Magee again stood trial in Las Vegas, for killing John Lassetter in the lobby of the Meadows (El Fidel) Hotel 10 months earlier.
Charette Lakes: No reports from anglers this week.
Cimarron River: Trout fishing was good using tricos, brassies, elk hair caddis, Cimarron specials, red copper John Barrs, small gold Panther Martins and worms. Water flow below Eagle Nest Lake as of Monday was 22 cfs. Fishing at the Gravel Pit lakes was fair using salmon eggs.
"We were close enough to grab it, but we just didn't grab it," said team manager Rick Armijo of his Las Vegas Little League 11-12 All-Stars' run at the state title this past week in Taos.
Las Vegas reached the final four of the tournament, which brought the best majors division teams in nine districts from around the state.
The run ended Wednesday with a 14-2 defeat to Carlsbad.
"I'm real proud of the kids," Armijo said. "We had a great run. We were part of the last 48 players (in the age division) to be playing in the state."
Copyright © 2018
The Las Vegas Optic is your source for local news, sports, events, and information in San Miguel County and Las Vegas, NM, and the surrounding area.


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First Tutors helps you to find Channel Islands spanish teachers in your local area. Our service showcases Channel Islands spanish tutors offering popular subjects such as English, Maths and German, but also less common subjects like Psychology. Entrance exams, SATs, GCSEs and A-Levels local spanish tuition are all catered for throughout Channel Islands. Please use our service to search for Channel Islands home spanish tutors in your area and select which most fits your requirements.
This is the only place to find, through one search, the most suitable Channel Islands spanish tutor for your needs, whether you are looking for local spanish tuition to boost your child's spanish confidence, your own online spanish education or you are seeking adult / casual spanish education.
Our search will find Channel Islands spanish tutors in your area according to their charges and distance from you. Our subjects include English, Maths and Chemistry from primary level right through to GCSE, A-Level and on to degree level tuition.
If you are a Channel Islands spanish teacher wishing to offer your private spanish teaching services in Channel Islands or anywhere else across the UK, please register with First Tutors or find out more about what we offer here.


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For example, the closest Chevy's is now an hour away. :( I loved that place.
Why do chains I like completely vacate entire areas?
Have an opinion?
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Be the first girl to share an opinion
and earn 1 more Xper point!
What Guys Said 1
It is all about profit, if they make it they stay, if they don't they leave.00|00|0
Why is the food in Britain awful?
- Show AllShow Less
Yes @ sour cream and onion, but not wavy ranch.
Don't know anyone who works for them.
Why not?
Oh. What do you do?
And it's ridiculous that it's "conservative" to support gay marriage and abortion, and they follow this guy who did sick things just to fit in at University. UKIP should take over, or the Tories should get a better leader.
Click "Show More" for your mentions
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Cecelia "Red" Dulin
- 69 years old
- Date of birth: Apr 25, 1946
- Date of passing: Jul 30, 2015
|Let the memory of Cecelia be with us forever
This memorial website was created in memory of our loved one, Cecelia Dulin, 69, born on April 25, 1946 and passed away on July 30, 2015. We will remember her forever.
Memorial Tributes
I don't even know what to say to be honest. I think I said it all already. But since I can't sleep even though I'm exhausted, I just wanted to talk to you. Like old times. But now I'm too sad to write much so I'll just tell you how badly I miss you, how sorry I am for ever being rude or dismissive to you and I love you.
If you see dad, you know what to do.
Love you <3
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What we do
Our aim is to bring Australians truly exciting, innovating and outstanding products.
We think presentation is important, however, it is the liquid inside our fancy packaging that we pride ourselves on.
Every product that we find undergoes immense scrutiny and only the finest quality products will enter our portfolio.
Call us+61 419 871 607
Mail email@example.com


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Does that first look in the mirror make or break your entire day?
It hurts my heart to say that you're definitely not alone. Body image can carry enormous weight on our sense of self-worth and, therefore, our mood and satisfaction levels in life.
What if I told you that body image actually has less to do with the shape of your body than you think?
The trigger that drives us to think that we're worth more one day than the last – when our weight is more or less the same – has nothing at all to do with our body's shape.
What I've learned to be true is that much of the time, our bodies actually look the same as they did yesterday to outside eyes. We, however, see something different because our minds are trying to protect us.
The Distorted Cycle
Throughout our lives, we're taught that there is an ideal way to look. Through subliminal and straight-forward messaging from media and society, we learn that people who don't look this way are everything we don't want to be. Maybe a few of these descriptions resonate with you: lazy, messy, disgusting, no willpower, weak, sad, unlovable, unhealthy, careless, etc. In a word: bad.
Our minds are complex things, but when we're fed that kind of information day in and day out for our entire lives, one belief becomes simple: if you are not the ideal body type, you are bad.
We've spent so much of our lives trying to make sure that we never ever fit the horrible descriptions we've been taught about those who have body types different than the ideal. We devote countless hours and energy to creating the perfect body. Many of us become obsessive about it. Because we've have focused so heavily on "fixing" our bodies, our perspective automatically shifts towards that when things go wrong.
We've learned to blame our bodies when we can't control what's wrong. It's a coping mechanism.
Here's how it works:
- We have a stressful day, week or year because of things outside of our control.
- We wake up one day (or many), see ourselves in the mirror and are aghast at the way our bodies look.
- We blame our bodies for this helplessness we feel.
- Now we have a reason for why we feel out of control: our bodies are obviously to blame.
When we feel out of control in our lives, we default to this simple explanation so we have something to control; it's called "displacement." It's a term from Freudian psychology that means: "an unconscious defense mechanism whereby the mind substitutes either a new aim or a new object for goals felt in their original form to be dangerous or unacceptable." [source]
Being out of control for many of us is a completely unacceptable way to be. We've just never learned how to deal with the parts of life that we can't fix. If you're a do-er, this might be one of the most uncomfortable sensations for you.
In an attempt to give you something to control, your mind displaces your helplessness onto poor body image.
Another "bonus" is the shame that comes along with it because our minds know that this shame is comfortable in its familiarity. Many of us have never lived without it! Our minds determine that giving us both this digestible reason for our helplessness (our bodies), and the shame that we're so familiar with is a protective measure.
While it seems a twisted way to care, that's exactly what our minds are doing for us: taking care of us. It's sweet, really. Now that you're aware of how this cycle works, however, you can shift it to serve you.
How To Break The Cycle
So how do we heal this? It all starts with awareness. If you notice that you have more negative body image thoughts than usual, take a moment to explore what else has been going on in your life:
- Are you stressed?
- In what areas of your life do you feel chaotic or helpless?
- Are you dealing with a lot of emotion you just don't know how to feel?
Any of these experiences could lead you into feelings of helplessness and, therefore, worsening body image. Acknowledging that issues outside of your control could be leading you to have negative body image might just help alleviate the burden. Here are a few prompts to consider when you become aware of helplessness:
What if it isn't your fault? What if it doesn't mean anything about you as a person and your ability to exist? What if it's just completely outside of your control?
Allow yourself to find some relief by considering those questions.
Then, explore some ways to get comfortable with being out of control. It's the scariest thing if you've never done it which is why I always recommend working with a licensed therapist for this kind of work. If you want to read more about how I recommend beginning to release control, check out this post and if you're feeling uncomfortable emotions, this one.
What If Your Body Really Is Changing?
Before I answer this, I have another question for you: Have you ever gotten happier in life by shaming yourself into results?
I'm going to venture a guess and say, "NOPE."
Out of all my clients over time, I've never experienced one that shamed herself into long-term positive results. Whether that's weight loss, muscle gain or even just balancing out her diet. There's nothing good to be gained from shame and guilt.
By accepting our mind's interpretation that our bodies are "bad" as they are, we envelope ourselves in a cloud of shame that negatively affects every action we take towards our goals.
That's when resistance starts to crop up, desperation takes over and everything feels heavy and difficult. It's almost impossible to get healthier when you're surrounded by this shame cloud.
Instead of defaulting into this shame, how would it feel to acknowledge your body image, then explore the idea that this is where you are today and that's ok? Telling yourself that how you look today isn't resignation to being this shape forever. That's a common misconception. Finding forgiveness for yourself where you are now allows you to take the next aligned step forward without all the junk that makes it difficult (like resistance, desperation and shame).
Today doesn't determine your forever. How you look has nothing at all to do with who you are as a person. So you're not where you'd like to be yet; what's one thing you can do to truly love yourself today so you might feel better? Because when it comes down to it, that's all we really crave by wanting to be more "in shape" anyway: more Love. For ourselves and from others. Why wait until you look a certain way to receive it?
If you notice that your body has consistently been changing in a way that doesn't feel healthy, it might benefit you to try out mindful eating. It's a great way to get in touch with your actual hunger and bring more Love into the relationship you have with your body without depriving and restricting yourself (which leads directly to overeating). For a few immediate tools, check out my free Emotional Eating Toolkit.
To Wrap It Up…
In conclusion, your distorted body image is not your fault. You haven't done anything wrong. Your mind has just learned to support you in a really weird way… but she can learn how to do differently from now on.
While I understand that this displacement phenomenon is not the only cause of negative body image, it can be helpful to recognize that it does happen in many of us.
I hope this brought you some relief today. If you have any questions, please don't hesitate to reach out to me on Twitter, Facebook or Instagram.
Stay strong,


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Food just tastes better when it's fun to look at doesn't it?!
Mouse (but they say it's an alligator) from Healthy Snacks for Kids 😉
Birdies in a Tree from Meet the Dubiens
Angry Birds Bento from My Mealbox
Cute little chicks from just JENN
Author: Allison Waken
Allison Waken is a wife, mom of boys and Phoenix, AZ native. She has been creating inspiring content for All for the Boys since 2011. Allison loves travel, movies and spending as much time as possible with her family while she can!


text: > After all its "just" maintaining a state and rules for possible transitions. I've implemented my own web framework (just like every other Python developer ;-) and I've done my own finite-state-machines for workflows. Web Frameworks are large, error-prone and have a lot of nuanced details. Workflows done with FSMs are much more transparent and require far less work. Each time I've created an FSM, it's been a custom solution exactly matching the problem-domain, and taking less than an afternoon to implement (both coding the solution, and populating the state & transition tables). Thus I'd say a web framework is multiple orders of magnitude more complex than a custom rule engine. -tkc