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Backcountry Access Float 32
Where to Buy
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Retailers' Descriptions
Here's what other sites are saying:
Please note that the Float 2.0 Cylinder IS NOT INCLUDED with this pack, and must be purchased separately. Please download or print the Backcountry Access Avalanche Airbag 101 document, and review it in detail before using this avalanche pack. Features of the Backcountry Access Float 32 Airbag Pack 150-liter Airbag not only decreases your burial depth but also protects your head and neck from trauma in an avalanche Dedicated shovel/probe pocket Helmet and diagonal ski carry system Padded waist belt pockets Hydration compatible sleeve ice ax loops Load lifters Lined goggle pocket Please note that the Float 2.0 Cylinder IS NOT INCLUDED with this pack, and must be purchased separately.
- Moosejaw
The Backcountry Access Float 32 avalanche airbag is designed for pros, guides, and dedicated backcountry skiing and snowboarding junkies carrying the full quiver of essentials. Creating buoyancy and helping you float to the surface, the 150-liter airbag not only decreases your burial depth but also protects your head and neck from trauma in an avalanche.
- Mountain Gear
Skiing in the backcountry comes with the inherent risk of exposure to avalanche terrain. Should you find yourself caught in a slide, the BCA Float 32 Airbag Backpack is a tool that offers a drastically increased chance of survival. Pull a trigger on the shoulder strap, and a highly durable airbag quickly inflates behind your head, decreasing your odds of burial in avalanche debris by increasing your overall buoyancy. Even with this technology, the Float Backpack still offers all the storage you need for your shovel, probe, goggles, and other gear--here's to the evolution of safety beyond the rope.
- Backcountry.com
Backcountry Access Float 32 Avalanche Airbag Backpack. Designed for guides and pros, this Backcountry Access Float 32 Avalanche Airbag Backpack offers the exceptional protection of an integrated airbag system while delivering abundant features and space. The Backcountry Access Float 32 is designed for pros, guides, and dedicated backcountry skiing and snowboarding junkies carrying the full quiver of essentials. Creating buoyancy and helping you float to the surface, the 150-liter airbag not only decreases your burial depth but also protects your head and neck from trauma in an avalanche. The Float 32 will carry all your common winter backcountry tools while supplying ample space for a first-aid kit, glacier travel equipment, and even supplies for extended multi-day trips. The trigger can be set in either the left or right shoulder strap. Backcountry Access' backpack straps on this pack are hydration compatible and the airbag system is removable, making it the most versatile airbag backpack on the market. The BCA Float Cylinder is sold separately. BCA's 200 refill locations around the world make it the easiest refillable airbag system available. The Float Airbag system does not guarantee survival in an avalanche incident. Educate yourself, make good decisions, and know before you go. The Float airbags are the lightest and most affordable on the market. The pack features big volume, load lifters, new external shovel/probe pocket, waist belt pocket, lined goggle pocket, hydration sleeve, diagonal ski carry, ice axe carry, helmet carry and optional snowboard carry attachment (sold as accessory). The system uses a 2,700 psi compressed air cylinder, single-chamber airbag and patented super-efficient venturi system. BCA has a rapidly expanding network of convenient Float refill and cylinder exchange centers. Pack Materials: Mainly durable 330 Double Rip-Stop and 840 Ballistic Nylon. Pack Volume: 1,593 cubic inches / 32 liters. Weight: 6.6 pounds / 105.6 ounces / 2,993.7 grams (entire system including cylinder). 5.2 pounds / 83.2 ounces / 2,358.7 grams (pack, airbag, engine, no cylinder). 3.6 pounds / 57.6 ounces / 1,632.9 grams (pack only, no airbag, engine or cylinder). Warranty: 3 Years. Torso Length: 18-23" / 45.72 cm - 58.42 cm. Bag Height: 23" / 58.5 cm. Bag Width: 11.5" / 29 cm. Bag Depth: 6.5" / 16.5 cm
- Tahoe Mountain Sports
VERSATILE AND REVERSIBLE, THE CAMEO SCARF WILL WIND ITS WAY INTO YOUR DAILY ROUTINE WITH ITS TWO-IN-ONE STYLE. A FLORAL PATTERN IS FEATURED ON ONE SIDE, AND A PLAID PATTERN APPEARS ON THE OTHER. BRAIDED FRINGE ACCENTS EACH END. New double weave wool scarf with two distinct looks floral on one side and plaid on the reverse side Braided fringed edge Dimenisons: 24 x 71 ( 61 cm x 180 cm)100 Wool
- Bentgate.com
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$412.50 - $549.95


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Granite Bathroom Tiles
Buy Granite Tiles for Your Bathroom
Granite tiles are an excellent choice for bathrooms and we have plenty of premium options available at Tilesoutlet. These highly durable natural granite tiles are a great option for floors and walls in both domestic and commercial bathrooms. You can select from classic varieties like Absolute Black, Galaxy Granite, Multi Red and Kashmir Gold, plus many more. Granite provides a distinctive style in any bathroom interior.
Granite Bathroom Wall Tiles
Create a stunning bathroom wall design, shower surround or backsplash feature with high quality granite tiles from this section. Granite wall tiles combine well with popular bathroom features like chrome handles, glass screens and white panels. You can select from a variety of granite tile sizes including large format 600x600mm tiles and smaller 305x305 tiles.
Granite Bathroom Floor Tiles
Natural stone granite is extremely hardwearing and is recognised as a superb flooring material. Granite tiles from this collection add style to bathroom floors and are also low maintenance and easy to clean. As a result of its longevity and unique appearance, granite floor tiles are acquired for bathrooms within commercial properties like shops and restaurants.

subset: commoncrawl


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Find the best Garages and Car Repairs in Islington with Who Can Fix My Car.
Enter your registration to compare prices and reviews and find a great garage today.
Find the best Garages and Car Repairs in Islington with Who Can Fix My Car.
My son was ill on the morning I was scheduled to bring the car in. Called Ade and he kindly picked my car up from my house. Clutch replaced at agreed quote. Cannot fault the customer service.
Good work done on time for the quoted price, Recommended.
The quote was honoured, it took about as long as expected. I would definitely recommend this garage & will use it again!
very friendly and helpful
Good garage, honest and reliable owner. I will use this garage in the future. Thanks Ade
Absolutely brilliant service. Thoroughly professional, kept me updated throughout despite being during the Xmas period. Work was immaculate on the car. Would 100% recommend to anyone.
Great service. They kept me informed the whole way through. Cleaned inside and out. Would definitely recommend.
They did a great job and car looks like new. Pick up and collect too which was a big reason why I chose them. Very satisfied.
Great experience! I wanted to repair a stone chip and bonnet dent and Ray was a friendly chap answering any questions I had. My request was dealt with very fast and the repair process was explained very well and the job was complete within 4 days. I also had 3 other smaller stone chips which was also repaired too without me asking so now the bonnet looks completely new.
Very pleased with the job Ray and his team did. They had a very competitive price and the customer service was one of the best I have seen across several sectors. They work around your schedule rather than having you to move your schedule to their availability (something I have noticed with several other garages). Great job done with my car. They even delivered my car back to my house.
Great customar service very helpful , thankQ guyz
Great job 10/10!
Really happy with the service provided, the work was done faster then expected and the mechanics where very professional and honest. Highly recommend.
I received fantastic customer service. The mechanic had me up and running in no time.
Excellent service and response was immediate in 5 minutes once submitted the quotes. Some good advice my the mechanic
Experienced garage who dealt with a number of separate issues very well, including ordering in specialist parts. Returning to find the car washed and vacuumed was a nice touch. 8 stars rather than 10 purely for the fact that I was informed they would need to keep the car overnight and it was only when I put my foot down that the job was finished on the day. Normally I'd accept that but I had dropped the car off at 7am so clearly had hoped to be first in the queue!
Very good on the whole, the mechanic was able to fix the problem within half an hour. Branko very helpful and honest. Will definitely go again. They are a little pricey compared to the other estimates I received but you are guaranteed a very top service.
They were great, really nice people, very quick, would use again.
Very happy with service, done a decent job, would use again.
Pleased with the work done, would use again.
Very efficient, job perfectly & professionally executed in less then half an hour. And above all very polite. Worth every penny.
Emory came to my house and checked my keys - the spare one worked and he didn't charge me. Excellent service.
Hi, I would like to rate Emre, from Herts and London car keys as 5/5. The service and efficiency was excellent. To begin with, I got a prompt response from my internet enquiry, and when I called Emre, he came promptly, within the hour, he was working on my car. My car would not start at all and had an immobiliser problem and couldn't get it to start for the past 3 days. Emre understood the problem, was upfront and very honest about it, and knew exactly what to do. He charged me £90, exactly as his original quote, with no extra or hidden costs, and it was such a relief to see my car starting and running again. Thank you so much, Emre, anyone with an immobiliser problem, I would highly recommend Emre.
Great service. Came to the house on a Sunday and had fixed it within half hour. Lovely bloke very friendly and great communication. Wouldn't hesitate to recommend
I would definitely recommend Vagpro ! I am very pleased with the service I received from the engineer Khurrum, he was very polite, friendly & above all knowledgable solving the problem quite quickly once he had the car all set up on his diagnostics machine, talking through step by step so I could try to understand what was happening. This guy is very good at what he does and I feel was worth every penny ( considering other quotes) for the work he did to my car, also sorting a small problem with my reversing sensors at no extra cost. Vagpro done what they said ! Fix the roof on my A4 Audi Convertible.
Fantastic customer service from the moment Vagpro contacted me to the moment i took i left his garage. Very professional, courteous and refreshing to find a person that can finish the job with conviction. I had crazy qoutes to even look at my car and diagnose the faults but i got my car foced for less than those qoutes including the parts. At a time when i almost gave up tryig to find a reliable and honest person to fix my car Vagpro renewed my confidence in the car mechanic industry. Keep up the good work!
Took my car in at 4pm and was finished by 6 samd day. Highly recommended!
Great communication and service from Inderjit, - changed both headlight units and the number plate light unit on my 2006 Nissan Micra K12 sport+, so it then passed the MOT. Would recommend.
They were great, checked my car properly and they were very honest. It turned out to be just a fuse were they changed it for me instead of a new alternator were I was told from a different mechanic. I have recommended them to all my family and friends I'm very happy. :-)
4 fab wheels were a pleasure to work with! Billy was extremely friendly and helpful, explaining everything very clearly and taking very good care of the car. He contacted me periodically throughout the process to keep me updated on the progress of the repair and was always available to answer my questions. I would strongly recommend him and his team to anyone who wants a trustworthy and professional service! Thanks for everything, Billy!
The cost of servicing, MOT and repairs differ across the country. Labour rates will be higher or lower depending on where in the country you are. Take a look below at the average quote in Islington on WhoCanFixMyCar.com for some of our top service types.
|Service Type
|Average Price
|Get Quotes
|Full Service
|Full Service and MOT
|Clutch Replacement
|Brake Pads and Discs
|Cambelt Change
|Mobile Mechanic
|Clutch Repairs
|Head Gasket
|Aircon Regas
This table uses data from quotes provided on WhoCanFixMyCar.com between 25/10/2017 and 23/01/2018.


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I was going to post a follow up entry or two on data science, but then a red-blooded American homophobic terrorist went and murdered fifty people in Orlando with a military weapon, because they were GLBTQ, or because they were Latinx, or both.
And another one got stopped on his way to Pride in LA, or he might have succeeded in doing the same.
Love your fellow humans. Love their love; love that they love; love that they love who they love.
Be humane. Be humble. Be human. Stop the killing.


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Download Bonsai Collection Pro apk 3.2 free for Android smartphone
Posted by MortyLabs in Tools | July 6, 2015 | 3 Comments
Apk file size: 1.8 MB
Bonsai Collection Pro is an application that allows you to manage and take care of your bonsais with ease.
- You can manage all your bonsais in one application.
- You can add tasks for each of your bonsais.
- You can add,delete or edit your bonsais or your favorite species.
- You can keep track of your bonsais with your own pictures record.
- You can add general tasks for the whole year.
With Bonsai Collection Free you can manage your bonsais with your Android device!
Share it!
Follow us on Facebook! https://www.facebook.com/BonsaiCollection
If you want you can try Free version of Bonsai Collection here: http://goo.gl/MmBzXK
Whats new
Solved crash problems.
MortyLabs part of our Tools and have average installs from 100 to 500. Last Update July 6, 2015. Google play rating is 85.3333. Current verison is 3.2. Actual size 1.8 MB.
Similar applications
3 Comments
Jason Horton
Corrupts my photos. Old version was better.
Best I have found This is the best Bonsai this is the best Bonsai database app I found to date. I had a previous app that does not work very well with the Galaxy S7 so I had to find a replacement for it.
Ell Stockill
Better than anything else Good but a few bugs with the image uploader bad for uploading multiple images for one tree. Also the species list should automatically reorder alphabetically when you add a new species. Other then that great


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I'd like Meagan to come live next door to me. Really.
It's my pleasure to introduce Meagan Francis, writer, mom of five and author of the new book, The Happiest Mom: 10 secrets to enjoying motherhood. Are you a little too hard on yourself? Do you grumble often about being a mom? Well, grab a cup of coffee and sit a spell. Meagan is warm, funny and has loads encouragement for you as a mom in HM. As an experienced mom, I was a little hesitant when I started reading it because what could she tell me that I don't know? Oh.My.Was.I.Wrong! Meagan has a heart for moms and fabulous ideas to enjoy your family today. Below is a brief interview I had with her:
Comparing and measuring ourselves with other moms is a large contributor to our own guilt. What simple steps can a mom do to stop this behavior?
The second thing we can do is not take part in cutting other moms down. Even reading judgmental blog posts makes me feel uncomfortable, because I start to wonder what people would say about me to their friends or blog readers if they saw me out with my kids or could peep into my windows. And when you feel judged–even potentially judged!–it starts that whole cycle of defensive judgment on your own part. It's a losing game, because none of us are all right and very few of us are all wrong.
The more we extend grace to others the easier it is to extend it to ourselves. And the more we extend grace to ourselves the easier it is to extend it to others.
One way to figure out if I'm unfairly finger-pointing is to ask myself questions like "Does this really matter?" "What role did I play in this?" "Am I being fair? "How could this go differently next time?" "What's the real issue here?"
I think in any situation where you're feeling uncomfortable, guilty, or angry, asking yourself questions like these before you react can help you get to the heart of the issue and gain perspective.
Here's HOW to WIN:
1. Leave a comment telling me how you can be a happier mom today.
2. Follow me on twitter and leave a comment telling me you did. (@JanUdlock)
{If you're already a follower, leave a comment. You guys get one too!}
3. Facebook about the giveaway, and leave a comment, telling me you did.
This counts as three entries and three chances to win. And as always, thank you again, dear mom, for stopping by.


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BouncyMC - 1.7.10/1.8
Now we have all seen those servers packed full of people, spamming the chat, stripmining and being a complete pain. the idea of bouncyMC is to be less crowded. we are also a non profit server. We dont beg for donations (Votes Are Nice Though!) as donations are a simple way of making someone OP also PVP is enabled so you better patch up quickly. We also have grief prevention that stops any damage caused to your creations and in the rare case that there is a grief we will happily rollback the griefers actions and server moderators are always happy to help.
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Okay my friends, I vow (get it? wedding theme?!) to write you some lower calorie treats after this week, but first I want to wrap up the recipes I promised you from my sister-in-law's bridal shower.
These sugar cookies were such a hit that one of the guests requested an order for a shower she's throwing next month!
When they first came out of the oven, I felt like an artist sitting in front of a canvas. With so many options right at my fingertips, I carefully decided on a vision that would be both beautiful and possible to execute.
I decorated the brides to match the dress in my red velvet cupcakes display, with white non-peril embellishments and a purple sparkly belt around the waist.
Each cake was done with delicate, edible pearls to signify the layers, and had two bright pink heart sprinkles on top.
The rings were a bit more playful than sophisticated, reminiscent of playing dress up as a child. I used bright yellow and blue writing icing to pipe the gold band and diamond, with blue glitter to show off some sparkly bling. For another variation using candy buttons (!) check out my diamond ring sugar cookies on CT Style News 8!
For an extra special touch, I cut out a heart-shaped cookie that I personalized just for the bride. This way if she were to call dibs on dessert and say, "that sugar cookie has my name on it," she would be speaking literally.
When researching recipes, I saw this one had rave reviews, and I can understand why. The dough was so malleable and easy to work with, making rolling out various designs that much more fun.
The cookies themselves are subtly sweet, balancing them ever so perfectly against the royal icing I will share with you tomorrow.
They are not only yummy to eat, but a lovely focal point for your next party!
*Makes 30 cookies*
3/4 butter, softened
1 cup white sugar
2 eggs
1/2 teaspoon vanilla extract
1 1/2 cups all-purpose flour
1 teaspoon baking powder
1/2 teaspoon salt
In a large bowl, cream together butter and sugar until smooth.
Beat in eggs and vanilla.
Stir in the flour, baking powder, and salt.
Cover, and chill dough for at least one hour (or overnight).
Preheat oven to 400°. Roll out dough on floured surface 1/4 to 1/2 inch thick.
Cut into shapes with cookie cutters of your choice and place 1 inch apart on ungreased or parchment lined cookie sheets.
Bake 6 to 8 minutes in preheated oven. Cool completely before frosting with royal icing (recipe tomorrow) and decorating.
Come back tomorrow and I'll show you how to decorate the cookies!


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What would this "trace / risk" appearing in the picture? It would be highlighting the object that is far away?
If there is a way to take?
Request ID R595401
Job ID J274764
Object Type COMET
Object ID C/2015 F4 (Jacques)
Object Name
Exposure Time 180000 ms
Filter Type Colour
Dark Frame Instant
Site Name Tenerife
Telescope Type Name Cluster
Telescope Name Cluster Camera
Request Time 17:55 on Thursday 17 September 2015 (16:55:25 UTC)
Completion Time 21:47 on Sunday 20 September 2015 (20:47:44 UTC)
Status Complete
Edinaldo Oliveira


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We recently identified intersectin, a protein containing two EH and five SH3 domains, as a component of the endocytic machinery. The N‐terminal SH3 domain (SH3A), unlike other SH3 domains from intersectin or various endocytic proteins, specifically inhibits intermediate events leading to the formation of clathrin‐coated pits. We have now identified a brain‐enriched, 170 kDa protein (p170) that interacts specifically with SH3A. Screening of combinatorial peptides reveals the optimal ligand for SH3A as Pp(V/I)PPR, and the 170 kDa mammalian son‐of‐sevenless (mSos1) protein, a guanine‐nucleotide exchange factor for Ras, contains two copies of the matching sequence, PPVPPR. Immunodepletion studies confirm that p170 is mSos1. Intersectin and mSos1 are co‐enriched in nerve terminals and are co‐immunoprecipitated from brain extracts. SH3A competes with the SH3 domains of Grb2 in binding to mSos1, and the intersectin–mSos1 complex can be separated from Grb2 by sucrose gradient centrifugation. Overexpression of the SH3 domains of intersectin blocks epidermal growth factor‐mediated Ras activation. These results suggest that intersectin functions in cell signaling in addition to its role in endocytosis and may link these cellular processes.
Src homology 3 (SH3) domains, 50–70 amino acid modules, mediate protein–protein interactions by binding specific proline‐rich peptide sequences within cellular ligands. A well studied example of an SH3 domain‐mediated interaction is the recruitment of the Ras guanine‐nucleotide exchange factor, mammalian son‐of‐sevenless (mSos), to the plasma membrane by the adaptor Grb2 (Baltensperger et al., 1993; Buday and Downward, 1993; Chardin et al., 1993; Egan et al., 1993; Li et al., 1993; Rozakis‐Adcock et al., 1993). mSos contains a proline‐rich C‐terminus that mediates its stable association with the SH3 domains of Grb2, a protein composed of two SH3 domains flanking a central SH2 domain (Lowenstein et al., 1992). Through its SH2 domain, Grb2 interacts with activated growth factor receptors either directly or through the adaptor protein SHC (Ceresa and Pessin, 1998), thereby recruiting mSos to the membrane where it, in turn, activates Ras (Aronheim et al., 1994; Quilliam et al., 1994). However, recent evidence suggests that mSos can be recruited to the membrane by additional mechanisms. Specifically, activation of Ras by mSos can occur in the absence of Grb–mSos interactions (Karlovich et al., 1995; McCollam et al., 1995; Wang et al., 1995), suggesting that other domains of mSos, such as its Dbl homology (DH) or pleckstrin homology (PH) domains, play important roles in mSos membrane targeting and activity (Chen et al., 1997; Qian et al., 1998), or that targeting of mSos to the membrane can be mediated through the actions of other adaptor proteins.
SH3 domain‐mediated protein–protein interactions also function in vesicular trafficking, particularly in endocytosis (McPherson, 1999). For example, intersectin, a novel protein involved in clathrin‐mediated endocytosis, contains two N‐terminal Eps15 homology (EH) domains, a central helical region and five C‐terminal SH3 domains (termed SH3A–E) (Guipponi et al., 1998; Yamabhai et al., 1998). Intersectin is homologous to Dap160 in Drosophila (Roos and Kelly, 1998), as well as to Ese1 in mouse (Sengar et al., 1999) and EHSH1 in rat (Okamoto et al., 1999). An alternatively spliced variant of intersectin, referred to as intersectin‐long (intersectin‐l), contains a C‐terminal extension with DH, PH and C2 domains (Guipponi et al., 1998). Via its EH domains, intersectin interacts with epsin, a protein that binds to clathrin and the clathrin adaptor complex, AP‐2 (Chen et al., 1998; Hussain et al., 1999), and through its SH3 domains (Yamabhai et al., 1998; Okamoto et al., 1999; Sengar et al., 1999), intersectin interacts with synaptojanin and dynamin, two proteins with critical enzymatic activities involved in the formation of clathrin‐coated vesicles (Cremona et al., 1999; Sever et al., 1999). In addition, intersectin heterodimerizes through the formation of coiled‐coils with Eps15 (Sengar et al., 1999), another accessory protein in endocytosis (Salcini et al., 1999). Thus, intersectin is believed to function as a scaffolding protein that assembles certain proteins at sites of clathrin‐coated pit formation.
To study the role further of the SH3 domains of intersectin in endocytosis, we tested their ability to block transferrin receptor uptake using a permeabilized cell assay (Simpson et al., 1999). Intriguingly, while the SH3C and SH3E domains of intersectin, as well as the SH3 domains from the endocytic proteins endophilin I (de Heuvel et al., 1997; Ringstad et al., 1997), amphiphysin II (Ramjaun et al., 1997) and syndapin I (Qualmann et al., 1999), selectively inhibit late events of clathrin‐coated vesicle formation involving membrane fission, intersectin SH3A was unique in its ability to block earlier stages (Simpson et al., 1999).
To follow up on this observation, we performed overlay assays with a glutathione S‐transferase (GST)–SH3A fusion protein and identified an SH3A domain‐specific binding partner of 170 kDa (p170). Analysis of the optimal ligand preference of the SH3A domain suggested that p170 was mSos1, a result confirmed by a variety of methods. Like intersectin, mSos1 is enriched in nerve terminals, and the two proteins strongly co‐immunoprecipitate from embryonic brain extracts. Intersectin and Grb2 compete for binding to mSos1, and sucrose‐density gradient analysis indicates that in brain, intersectin and mSos1 form a stable complex that primarily excludes Grb2. Interestingly, overexpression of the SH3 domains of intersectin strongly attenuates epidermal growth factor (EGF)‐dependent GTP loading of Ras. Thus, it appears that intersectin may function as a scaffold molecule for protein components of both the endocytic machinery and signal transduction pathways.
Identification of an intersectin SH3A domain‐specific binding partner
In previous collaborative studies, we determined that the SH3A domain of intersectin is unique among a wide variety of SH3 domains from endocytic proteins, including the other SH3 domains of intersectin, in its ability to block intermediate steps leading to the formation of a sequestered clathrin‐coated pit (Simpson et al., 1999). We thus performed overlay assays using a GST–SH3A fusion protein in order to identify specific SH3A‐binding partners. The intersectin SH3A domain, as well as the SH3 domains from amphiphysin I and II and endophilin I, II and III, each interact with bands at 100 and 145 kDa (Figure 1). The 100 and 145 kDa bands are most likely to be dynamin and synaptojanin, respectively, which have been observed to interact with the SH3 domains used here in several studies (Micheva et al., 1997; Ramjaun et al., 1997; Yamabhai et al., 1998). Interestingly, SH3A also interacted with an additional band at 170 kDa (Figure 1). The 170 kDa band (p170) was readily detectable at concentrations of fusion protein as low as 100 ng/ml, but was not detected in overlay assays with the other four SH3 domains of intersectin nor with the SH3 domains from the amphiphysins or the endophilins, at the same concentrations (Figure 1 and data not shown).
GST–SH3A overlays of tissue extracts revealed that p170 was most enriched in brain, although significant levels were detected in all tissues tested, and in several tissues p170 was the only protein species identified (Figure 2A). Within the brain, subcellular fractionation revealed that p170 was present in both soluble and particulate fractions. Its overall distribution was similar to that of dynamin and synaptojanin (Figure 2B), proteins that are enriched in pre‐synaptic nerve terminals (McPherson et al., 1994a), although a small pool of the protein was found in the P1 fraction, possibly in association with nuclei.
Identification of p170 as mSos1
SH3 domains interact with proline‐rich peptides with the core sequence PXXP (where X is any amino acid). To define the ligand specificity of the intersectin SH3A domain, peptides were affinity selected from a phage‐displayed X6PXXPX6 peptide library (Sparks et al., 1996a). The peptides that were selected from the library (Figure 3A) share a six amino acid motif with the consensus Pp(V/I)PPR, where p is typically proline. This motif represents a class II ligand for SH3 domains (Feng et al., 1994; Lim et al., 1994) and overlaps with the specificity of the Src SH3 domain (Sparks et al., 1996a). A computer search of DDBJ/EMBL/GenBank revealed two matching sequences (PPVPPR) in mSos1 (Figure 3B), a protein of 170 kDa (Chardin et al., 1993; Li et al., 1993). To determine if these two sites serve as peptide ligands for the SH3A domain of intersectin, the two sequences, along with a third highly related sequence from mSos1 (Figure 3B), were tested for binding to the five SH3 domains of intersectin expressed individually as GST fusion proteins. As seen in Figure 3C, only the proline‐rich sequences within mSos1 that matched the defined consensus bound to the SH3A domain, whereas none of the peptides bound to the other four SH3 domains.
To determine if p170 is indeed mSos1, we performed GST–SH3A overlays on proteins immunoprecipitated from embryonic day 18 (E18) rat brain extracts with a polyclonal mSos1 antibody (C23). C23 efficiently immunoprecipitates mSos1 (Figure 4, top panel) and the immunoprecipitated protein is strongly reactive in a GST–SH3A domain overlay (Figure 4, middle panel). Furthermore, immunodepletion of mSos1 from rat brain extracts depletes p170 (Figure 4, bottom panel), confirming that this interacting protein is mSos1. Moreover, the tissue distribution of mSos1 as determined by Western blotting (data not shown) closely parallels that of p170 (demonstrated in Figure 2A), suggesting that mSos1 is equivalent to p170 in all tissues tested.
Intersectin and mSos1 interact in neurons
To explore the potential interaction between mSos1 and intersectin in situ, we first performed immunoperoxidase staining to examine the regional distributions of the two proteins. Intersectin and mSos1 were expressed in discrete and highly overlapping neuronal populations in rat brain cortex, caudate and ventral pallidum (data not shown). Previously, it was demonstrated that Dap160, the Drosophila homologue of intersectin, was present in nerve terminals from third instar larvae (Roos and Kelly, 1998). Staining of 1‐day‐old neuron cultures from the CA3 region of hippocampus revealed that intersectin is broadly distributed in neurons including an enriched pool in growth cones (Hussain et al., 1999; data not shown). Interestingly, like intersectin, mSos1 is present throughout the hippocampal neurons and is enriched at the tip of their growth cones (Figure 5A). This enrichment is due to an increase in the density of mSos1 positive puncta in the growth cone area (Figure 5B) as well as an increase in the content of mSos1 per puncta (Figure 5C).
Next we performed co‐immunoprecipitation experiments using a cytosolic fraction of E18 rat brain extracts. Two different antibodies against intersectin (2173, 2174) immunoprecipitate both the long (intersectin‐l) and the short (intersectin‐s) forms of intersectin and lead to a strong co‐immunoprecipitation of mSos1 (Figure 5D; note the enrichment of mSos1 in the intersectin immunoprecipitated samples relative to the starting material). mSos1 also strongly co‐immunoprecipitates with intersectin from a Triton X‐100 solubilized particulate fraction, suggesting that the proteins also interact at the membrane (Figure 5E).
Intersectin competes with Grb2 for binding to mSos1
To explore the relationship between mSos1, intersectin and Grb2, we immunoprecipitated mSos1 from soluble E18 rat brain extracts and performed Western blots with antibodies against the various proteins. Intersectin‐l, intersectin‐s and Grb2 all co‐immunoprecipitate with mSos1 (Figure 6). However, whereas intersectin‐l was enriched in the immunoprecipitate relative to the starting material, Grb2 was not (Figure 6), even though in overlay assays, GST–Grb2 and GST–SH3A fusion proteins interact equally well with blotted mSos1 (data not shown). One plausible explanation is that intersectin and Grb2 compete for binding to mSos1, and the greater co‐immunoprecipitation of intersectin versus Grb2 is due to intersectin being more abundant than Grb2 in the brain.
mSos1 contains many SH3 domain‐binding consensus sequences (i.e. PXXP). To determine if Grb2 and SH3A compete for binding to mSos1, GST–SH3A was used in overlay assays on E18 rat brain extracts in the presence of increasing concentrations of His6‐tagged Grb2. Addition of Grb2 significantly reduced SH3A binding to mSos1 in a concentration‐dependent manner, with binding completely eliminated at a molar ratio of 5:1 (Figure 7). Thus, Grb2 and SH3A may compete for binding to the same or overlapping site(s) in mSos1 although steric hindrance cannot be excluded. Previous mapping experiments have demonstrated that Grb2 binds to human Sos1 at three sites (PXXP cores at amino acids 1151–1154, 1212–1215 and 1290–1293) (Li et al., 1993; Rozakis‐Adcock et al., 1993). The first and third of these sites are the same as those predicted by phage‐display experiments to be ligand sites for the SH3A domain (Figure 3).
To explore this issue further, we infected undifferentiated PC12 cells with an adenovirus construct encoding the five tandom SH3 domains of intersectin fused at their N‐terminus to green fluorescent protein (GFP) (GFP–SH3A–E). Following expression of the construct, soluble cell extracts were prepared and processed for anti‐GFP immunoprecipitation followed by blotting for co‐immunoprecipitating mSos1. The anti‐GFP antibody immunoprecipitates the GFP–SH3A–E fusion protein (which migrates with the expected molecular mass of 85 kDa) (Figure 8, middle panel) and leads to a strong co‐immunoprecipitation of mSos1 (Figure 8, top panel). However, Grb2 is not detected in the immunoprecipitated samples (Figure 8, bottom panel), consistent with the observation that intersectin and Grb2 bind competitively to mSos1.
We next investigated the association of intersectin with mSos1 by subjecting a soluble rat brain protein extract to sucrose‐density gradient centrifugation and analyzing the distribution of the proteins by Western blotting (Figure 9, top panels; the gradient was fractionated from the bottom such that lower fraction numbers correspond to a greater density of sucrose). Intersectin‐l was most abundant in fraction 9, but was detectable even in the densest gradient fractions. Interestingly, mSos1 migrated on the gradients with a molecular mass that was similar, but not identical, to that of intersectin‐l. Both intersectin‐l and mSos1 exhibited higher native molecular masses than either clathrin triskelia (∼650 kDa) or the AP‐2 heterotetramer (∼300 kDa), the migratory positions of which were revealed by Western blots of the clathrin heavy chain or the α‐subunit of AP‐2, respectively. Intriguingly, Grb2 remained at the top of the gradient and was effectively separated from mSos1.
To confirm that the extremely large native molecular mass for mSos1 was due, at least in part, to its interactions with intersectin, we performed co‐immunoprecipitation experiments from the gradient fractions (Figure 9, bottom panels). mSos1 could be co‐immunoprecipitated with intersectin from each of the gradient fractions containing the two proteins. Overall, the distribution of mSos1 seemed to parallel more closely that of intersectin‐l than intersectin‐s. Whether this reflects a relatively higher affinity of intersectin‐l versus intersectin‐s for mSos1 or is due to the apparently greater abundance of intersectin‐l in brain is not known. Regardless, these data suggest that in brain, a pool of the mSos1 protein is in a stable, large molecular weight complex that includes intersectin but that predominantly excludes Grb2.
Overexpression of the SH3 domains of intersectin blocks Ras activation
To determine if the SH3 domain‐mediated interactions of intersectin with mSos1 might affect Ras activation, we measured the levels of Ras–GTP in cells with a GST fusion protein encoding the Ras‐binding domain of Raf1. This fusion protein binds to Ras–GTP but has significantly weaker affinity for Ras in its GDP‐bound form (de Rooij and Bos, 1997; Marais et al., 1998). In HEK‐293 cells treated with EGF for 2 min, a substantial pool of the total cellular Ras binds to GST–Raf1 beads as detected by Western blots with a Ras monoclonal antibody, whereas little binding is detected in the absence of EGF stimulation (Figure 10, top panel). Overexpression of GFP has little effect on the ability of EGF to stimulate GTP loading on Ras (Figure 10, bottom panel; note that the ratio of Ras bound to the beads relative to that in the starting material is similar to that seen in the EGF‐challenged non‐infected cells in the top panel). In contrast, overexpression of the GFP–SH3A–E construct sharply attenuates EGF‐activated GTP loading on Ras (Figure 10, bottom panel). There is no effect of the overexpression of either construct on the basal activity of Ras measured in the absence of EGF (data not shown).
The identification and characterization of accessory proteins in endocytosis has received a great deal of attention in recent years. One of these proteins has been identified in Drosophila, Xenopus and mammals and is variously referred to as Dap160, intersectin, Ese and EHSH (Guipponi et al., 1998; Roos and Kelly, 1998; Yamabhai et al., 1998; Hussain et al., 1999; Okamoto et al., 1999; Sengar et al., 1999). This family of proteins contains two EH domains, followed by a putative helical region and four to five tandem SH3 domains. Intersectin is localized in part to clathrin‐coated pits and interacts in vivo through its EH and SH3 domains with several proteins involved in clathrin‐mediated endocytosis. In addition, intersectin interacts with another accessory component of the endocytic machinery, Eps15, through its coiled‐coil region (Sengar et al., 1999). Thus, intersectin may function as a scaffolding protein in the assembly of clathrin‐coated pits.
We have now identified mSos1 as a protein that interacts with the SH3A domain of intersectin. Previously, Leprince et al. (1997) had isolated amphiphysin II in a two‐hybrid screen using the proline‐rich C‐terminus of human Sos1. In experiments reported here, we have found in a side‐by‐side comparison using overlay assays that the SH3A domain of intersectin can bind mSos1 better than the other four SH3 domains from intersectin or than the SH3 domains from amphiphysin I and II and endophilin I, II and III. These data suggest that intersectin can bind mSos1 with higher affinity than other SH3 domain‐containing endocytic proteins but do not rule out a role for amphiphysin II in mSos1 function. Intersectin and mSos1 are both expressed at high levels in growth cones of developing neurons, and immunoprecipitation analysis from embryonic brain extracts confirms that the two proteins are associated in situ. In fact, sucrose‐density gradients, coupled with co‐immunoprecipitation analysis, suggest that in brain, intersectin and mSos1 are components of a large molecular mass protein complex that primarily excludes Grb2. Thus, the interaction of the SH3A domain with mSos1 appears specific and is likely to be biologically relevant.
Given that intersectin is involved in the formation of clathrin‐coated pits, and that the intersectin SH3A domain interacts specifically with cellular targets that function early in the formation of a clathrin‐coated bud (Simpson et al., 1999), it is interesting to speculate that mSos1 may also play a role in clathrin‐coated pit formation, possibly through activation of Ras. Many vesicular budding events that are mediated by coat proteins are initiated by the activation of small GTP‐binding proteins through the actions of guanine‐nucleotide exchange factors (Schekman and Orci, 1996). Very recently, Nakashima et al. (1999) have demonstrated that overexpression of mutant forms of Ras, as well as the small GTP‐binding protein Ral, which plays a major role in mediating downstream Ras function (Feig et al., 1996), blocks the internalization of the EGF receptor. Furthermore, mSos1 can activate Rac (Nimnual et al., 1998; Scita et al., 1999), which has been implicated in transferrin receptor endocytosis (Lamaze et al., 1996). Finally, it should be noted that the long form of intersectin, which is generated by alternative splicing in neuronal tissues, contains DH, PH and C2 domains (Guipponi et al., 1998; Hussain et al., 1999; Okamoto et al., 1999; Sengar et al., 1999). Comparison of the primary structure of the DH and PH domains with other proteins suggests that the long form of intersectin may be a guanine‐nucleotide exchange factor for Rho. Further work is necessary to clarify the involvement of GTP‐binding proteins in clathrin‐mediated endocytosis.
Another possible function of the intersectin–mSos1 complex is to couple the molecular machineries for endocytosis and signal transduction. For example, it has been demonstrated that dynamin‐dependent endocytosis of the EGF receptor is necessary for EGF‐dependent activation of the MAP‐kinase pathway (Vieira et al., 1996). The ability of insulin‐like growth factor‐1 (IGF‐1) to activate the SHC/MAP‐kinase pathway, but not the insulin receptor substrate 1 pathway, is also dependent on clathrin‐mediated endocytosis of the IGF receptor (Chow et al., 1998). Furthermore, endocytosis of the β2‐adrenergic receptor is necessary for coupling to MAP‐kinase activation (Daaka et al., 1998; Luttrell et al., 1999). Specifically, overexpression of a mutant form of β‐arrestin, which prevents the β2‐adrenergic receptor from targeting to clathrin‐coated pits, blocks agonist activation of MAP kinase. Thus, it is possible that the clathrin‐coated pit can function as a membrane microdomain, directing the assembly of signaling complexes, much as has been proposed for caveoli (Anderson, 1998). In fact, activation of the EGF receptor can lead to the formation of signaling complexes that include mSos1 and which are localized largely in endosomes (Di Guglielmo et al., 1994).
Given the evidence for a link between endocytosis and signaling, it is interesting to speculate that intersectin could play an important role in bringing together endocytic proteins such as dynamin, with signaling molecules such as mSos1. In fact, our data demonstrating that overexpression of the SH3 domains of intersectin functions in a dominant‐negative manner to block EGF‐dependent Ras activation strongly support a role for intersectin in cell signaling. Moreover, human intersectin has been found to interact by yeast two‐hybrid screening with the proto‐oncogene product, c‐Cbl (Robertson et al., 1997), a tyrosine kinase substrate with ubiquitin ligase activity (Joazeiro et al., 1999), and transfection experiments have revealed that full‐length intersectin functions in cell signaling pathways leading to activation of the Elk‐1 transcription factor (J.O'Bryan, NIEHS, personal communication). Finally, in a genetic screen in Drosophila, Dap160, the Drosophila homologue of intersectin (Roos and Kelly, 1998), was selected as a negatively regulating component of the Sevenless receptor tyrosine kinase/MAP‐kinase pathway (F.Rintelen and E.Hafen, University of Zurich, personal communication). Thus, intersectin appears to have a dual function in both endocytosis as well as signal transduction pathways, and it may play a role as an interface between these two important cellular processes.
Materials and methods
Polyclonal antibodies against intersectin were described previously (Hussain et al., 1999). Antibodies against clathrin (Simpson et al., 1996) and α‐adaptin (Robinson, 1987) were a generous gift of Dr Margaret Robinson (Cambridge University). Polyclonal antibodies against mSos1 (C23) and GFP were purchased from Santa Cruz Biotechnology and Molecular Probes, respectively. Monoclonal antibodies against Grb2 and Ha‐Ras were purchased from Transduction Laboratories.
Generation of fusion protein constructs
GST fusion protein constructs encoding the SH3 domains of amphiphysin I (David et al., 1994), amphiphysin II (Ramjaun et al., 1997), endophilin I (Micheva et al., 1997), the individual SH3 domains of intersectin (Yamabhai et al., 1998) and full length Grb2 (McPherson et al., 1994b) were described previously. The SH3 domains of endophilin II and III were generated by PCR using full length cDNAs (Sparks et al., 1996b) as templates in PCR with Vent DNA Polymerase (New England Biolabs) and the following primer pairs: endophilin II, forward primer 5′‐GCGGGATCCGACCAGCCAAGCTGCAAG (nucleotides 919–936) and reverse primer 5′‐GCGGAATTCTCACTGAGGCAGAGGCACCAG (nucleotides 1104–1087); endophilin III, forward primer 5′‐GCGGGATCCGACCAGCCCTGCTGTCG (nucleotides 856–872) and reverse primer 5′‐GCGCCCGGGTCACGGAGGTAAAGGCACAATG (nucleotides 1041–1023). The resulting PCR products were subcloned into the corresponding BamHI–EcoRI and BamHI–SmaI sites of pGEX‐2T (Pharmacia Biotech Inc.), respectively. For His6‐tagged Grb2, the GST–Grb2 construct in pGEX2T was digested with BamHI plus EcoRI enzymes and the liberated insert was subcloned into pTrcHisA (Invitrogen) using the same enzymes.
Generation of recombinant adenovirus
A recombinant adenovirus encoding the five tandem SH3 domains of intersectin with an N‐terminal GFP tag was produced using the system of He et al. (1998). First, the appropriate region of intersectin was amplified by PCR using a full length cDNA (Yamabhai et al., 1998) with Vent DNA Polymerase (New England Biolabs) and with the forward primer 5′‐GCGCTCGAGGGAGAAAGCCCCTCTAACG (nucleotides 2352–2370) and the reverse primer 5′‐GCGGAATTCCTACGGTATTCCACCTGCTGG (nucleotides 3840–3823). The resulting PCR product was digested with XhoI and EcoRI and subcloned, in‐frame, into the corresponding sites of pEGFP‐C2 (Clontech) adding the N‐terminal GFP tag. The resulting fusion protein construct was liberated with NheI and SmaI and subcloned into the compatible KpnI and EcoRV sites of pShuttle‐CMV (He et al., 1998). The resulting plasmid was recombined with pAdEasy‐1 in BJ5183 cells and recombinant plasmids were selected on kanamycin and identified by EcoRI and BstXI digests (He et al., 1998). Recombinant adenovirus was produced and amplified in HEK‐293 cells and purified on CsCl2 gradients as described (He et al., 1998).
Tissue and subcellular distribution experiments
Various rat tissues were homogenized in buffer A (10 mM HEPES–OH pH 7.4, 0.83 mM benzamidine, 0.23 mM phenylmethylsulfonylfluoride, 0.5 μg/ml aprotinin and 0.5 μg/ml leupeptin). Post‐nuclear supernatants were obtained by centrifugation for 5 min at 800 gmax and were then separated into cytosolic and membrane fractions by ultracentrifugation at 205 000 gmax for 1 h at 4°C. Differential centrifugation of rat brain extracts leading to the defined subcellular fractions in Figure 2B was as described previously (McPherson et al., 1994a). Dissociated cell cultures were prepared from the CA3 and dentate regions of hippocampi from P1 rat pups as described (Hussain et al., 1999).
Overlay assays
Overlay assays with GST fusion proteins were performed as described (McPherson et al., 1994b). For competition experiments, GST–SH3A was mixed with increasing concentrations of His6‐tagged Grb2 immediately prior to addition to the nitrocellulose transfers.
Immunoprecipitation analysis
Cytosolic extracts, prepared as described above, were made to 1% in Triton X‐100 and precleared by incubation with protein A–Sepharose. The precleared samples were then incubated with various antibodies precoupled to protein A–Sepharose, and following an overnight incubation at 4°C, were washed three times in buffer A containing 1% Triton X‐100 before the proteins specifically bound to the beads were eluted and processed for SDS–PAGE. In some cases, membrane fractions, generated as described above, were incubated for 30 min at 4°C in buffer A containing 1% Triton X‐100, the samples were centrifuged at 205 000 gmax for 1 h at 4°C, and the soluble supernatants were used for immunoprecipitation. For immunoprecipitations from infected cells, PC12 cells were incubated with recombinant adenovirus encoding GFP–SH3A–E in 6‐well plates at a multiplicity of infection of 100. The medium was changed the next day, and the cells were left for an additional 48 h. The cells were then washed, scraped off the plate in buffer B (buffer A with 5 mM EGTA, 5 mM EDTA, 50 mM sodium fluoride, 20 mM sodium pyrophosphate and 1 mM sodium vanadate), lysed, Triton X‐100 was added to 1%, and the extracts were centrifuged in a Beckman TLA 100.2 rotor at 75 000 r.p.m. for 15 min. The solubilized extracts were precleared with protein A–Sepharose before the addition of anti‐GFP antibody precoupled to protein A–Sepharose. Following a 2 h incubation, the samples were washed three times in buffer B containing 1% Triton X‐100, and the proteins specifically bound to the beads were eluted and processed for SDS–PAGE.
Sucrose‐density gradient centrifugation
Proteins from a rat brain cytosolic fraction were layered onto linear gradients of 5–20% sucrose prepared in buffer A. The gradients were centrifuged in a STEPSAVER 50 V39 vertical rotor (Sorvall) at 195 000 g for 2.5 h and were then collected into 19 fractions of 2 ml each from the bottom. Aliquots of each fraction were analyzed by SDS–PAGE and Western blotting. For immunoprecipitations, 750 μl of each fraction were made to 1% in Triton X‐100 before the addition of protein A–Sepharose beads that had been precoupled to intersectin antibody. Following an overnight incubation at 4°C, the samples were washed three times in buffer A containing 1% Triton X‐100, and the proteins specifically bound to the beads were eluted and processed for SDS–PAGE.
Determination of SH3A binding specificity by phage display
GST–SH3A was used to affinity select phage from a library of bacteriophage M13 displaying combinatorial peptides at the N‐terminus of mature protein III. The library displayed peptides of the form X6PXXPX6, where X represents any of the 20 naturally occurring amino acids and P represents invariant proline residues (Sparks et al., 1996a). Three different 14mer peptides from mSos1 were fused to the N‐terminus of secreted bacterial alkaline phosphatase (Yamabhai and Kay, 1997) and their binding to various GST–SH3 domains, which had been immobilized on the surface of microtiter dishes, was monitored with the substrate p‐nitrophenyl phosphate. The optical absorbance (OD) of duplicate wells was determined with a plate spectrophotometer at 405 nm wavelength.
Ras assays
Recombinant adenoviruses encoding GFP–SH3A–E or GFP alone were added to HEK‐293 cells plated on poly‐l‐lysine substrate in 10‐cm2 dishes at a multiplicity of infection of 100. The medium was changed after 2 h and the cells were left for an additional 48 h. The cells were then transferred into serum‐free medium (along with non‐infected controls), and following an overnight incubation, were treated with 100 ng/ml EGF for 2 min at 37°C or were left untreated. The cells were then washed and immediately processed for Ras assays as described (de Rooij and Bos, 1997; Marais et al., 1998).
We would like to thank Dr Channing Der (University of North Carolina) for analysis of the intersectin‐l‐specific sequences, Dr Dengshun Miao (McGill University) for assistance with immunostaining of brain sections, Dr Margaret Robinson (Cambridge University) for the generous gift of antibodies to clathrin and AP‐2, and Dr David Kaplan and Stephen Morris (Montreal Neurological Institute) for gifts of PC12 cells and recombinant GFP adenovirus, respectively. We would also like to thank Drs Felix Rintelen and Ernst Hafen (University of Zurich) and Dr John O'Bryan (National Institute for Environmental Health Sciences) for discussion and for sharing unpublished results. Finally, we thank Drs Wayne Sossin, John O'Bryan and Ted Fon for advice and discussion. This work was supported by Medical Research Council of Canada Operating Grants MT‐13461 to P.S.M. and MOP‐36413 to D.B., and by grants from The Leukemia Society of America (New York, NY) and Novalon Pharmaceutical Corporation (Research Triangle Park, NC) to B.K.K. The work was also supported by a research contract from BiochemPharma (Laval, QC) to P.S.M. C.C.Q. is supported by NINDS grant #NS22807 to Dr Susan Hockfield of Yale University. N.K.H. is supported by a McGill University Faculty of Graduate Studies and Research Fellowship and P.S.M. is a Scholar of the Medical Research Council of Canada.
- Copyright © 2000 European Molecular Biology Organization


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Words containing enturist
Found 6 words containing enturist. Browse our Scrabble Word Finder, Words With Friends cheat dictionary, and WordHub word solver to find words that contain enturist. Or use our Unscramble word solver to find your best possible play! Related: Words that end in enturist
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Emilia Spivak, Russian actress
Emilia Spivak is a Russian theater and film actress.
Emilia was born on November 18, 1981 in Leningrad (now St. Petersburg). She is a daughter of theater director, People's Artist of Russia – Semyon (Solomon) Spivak.
In 2003 Emilia graduated from St. Petersburg Dramatic Academy. The girl took part in academic performances Older Brother, Kabare.
Emilia worked in Youth Theater on Fontanka and the Moscow Art Theatre named after Anton Chekhov.
In 2007 Spivak was the winner of the on-line voting and became The Actress 2007 for her role of Laura Wingfield in the play Blue Rose.
In 2009 Emilia received a silver medal of Federal service of punishment implementation for the contribution to the development of the penal system of Russia for her work in the TV series Gonchie (Trackhounds).
In 2010 the actress received theater prize Golden Soffit in the nomination The best dramatic actress for her role in the play Late Love.
Emilia starred in many films such as Secrets of the investigation (2001), Game on-line (2004), Streets of broken lamps (2006), Bridges of Love (2006), Three and Snowflake (2007), My dear man (2011), Sea Devils. Tornado (2013) and many others.
Personal life
There were rumors that Spivak had a love affair with Russian actor and TV presenter Ivan Urgant. But according to Emilia they were just partners in the film The Three and Snowflake.
The actress had a romantic relationship with actor Daniil Spivakovsky.
They say Emilia is married now, but who is her husband?
Her official site: эмилияспивак.рф


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STEVE INSKEEP, host:
Turning now to tweets - that is, of the instant messaging variety. Twitter is all the rage in social networking, although it has MORNING EDITION commentator John Ridley twittering with rage.
JOHN RIDLEY: At the risk of sounding like that old guy in "Gran Torino" telling those young punks to get off my lawn, it's gotten to the point whenever I hear somebody talking about Twitter or twittering or tweeting, it just makes my little tummy want to hurl. I haven't tweeted once in my life, but I'm sick of hearing about it already. What once may have been the cool way of letting a hundred people know you're about to go mow your lawn now has the feel of a used-to-be-fresh means of communication, so yesterday, like two-way pagers and AOL.
To be honest, I think Twittering jumped the shark long before ultra-hip CNN got into a Twitter match against super-down Ashton Kutcher, back when politicians started live-tweeting responses to the president's demi-State of the Union address, Twitter had already taken on all the cool of your mom getting a tattoo. I imagine, I hope, Twitterers are ultimately headed for the social networking retirement home that's the current residence of Second Life and MySpace. But my real issue with social networking sites isn't their faddishness. It's the hypocrisy that goes with them.
We claim to be a nation of people who take our privacy very seriously. Just mention the idea of warrantless wiretaps and expect to get hit up with a congressional investigation. But give somebody an avatar and a URL, and they can't tweet, post or hyperlink enough personal information about themselves to as many people as possible. Seriously, does valuable broadband space need to be taken up with announcements in that creepy Facebook third-person-ese John is enjoying two-for-one margaritas with the rest of the IT Team at TGI Fridays?
Where is the expectation of privacy anymore? Or, more correctly, where is the expectation that people will keep their private nonsense to themselves so that those of us who still like to communicate personal information with one person at a time don't have to get caught up in somebody else's email circles or listen to their one-sided cell phone conversations? No, I don't know what's hipper, to Facebook or to Twitter. I just know for me, personally, discretion never went out of style.
INSKEEP: Commentator John Ridley is founding editor of thatminoritything.com. And after ranting about tweets there for a little while, we need to mention that he has a blog called Visible Man at npr.org.
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INSKEEP: It's MORNING EDITION from NPR News.
Copyright © 2009 NPR. All rights reserved. Visit our website terms of use and permissions pages at www.npr.org for further information.
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I had been to have my hair done, and after I got home I realized it was fried!! (I won't go into my later converstion with my stylist!!) Anywaaaay, I drove over to Sally's Beauty Supply to see what they had to rescue my hair.
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I would only recommend this product for extremely dry and damaged hair, but it sure did restore the shine and managability to my over processed tresses! I highly recommend it for damaged hair.
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Download Hospital Escape apk 2.0.0 free for Android smartphone
Posted by funny games in Puzzle | March 9, 2015 | 15 Comments
Apk file size: 2.6 MB
A doctor mistake me and locked me inside the mental hospital
I don't know how to get out from this hospital
You are the only to save me
Help me to come out from here.
Need Adobe Air Plugin to play this game.
Whats new
Revised as per Google play policy terms
Improved User Interface
Enhanced Ads Enable
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funny games part of our Puzzle and have average installs from 50000 to 100000. Last Update March 9, 2015. Google play rating is 70.104. Current verison is 2.0.0. Actual size 2.6 MB.
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Runwell Mental Hospital Escape472 Comments
15 Comments
Ian Walker
Far too simple and short. Clever initial idea but never really got going, all those doors to explore but only one fuse box needs investigating. What a waste of graphics.
Gina Anderson
Not my fave I was disappointed in this escape game. No challenge and kind of a let down....sorry. I'm a big fan and so far I've only disliked this one.
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Can't open NEED Adobe air
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Soooo short But still luv funny games escape games! Plz keep'em coming
Christina Hunt
too easy too short and i was unable to enter correct code to escape...digits too small
Adore McKinnie
Not Bad At All This game is awesome...
Enoki Me
Very short but not bad. One glitch that tacked on some time is the wire wouldn't attach to the fuse panel till after a few attempts.
Tina Coronado
I love this game Its awesome
Valerie Edwards
Very easy
D Williams
Fast and fun Fast and fun
Tara-Louise Aiello
WTH! I usually love all the games from this developer, despite how short they are. But that was the worst game out of all of the ones I've played so far. It was so short, I wonder what the point was in wasting the time to make it. I don't mean to be horrible, but it would have been nice to add a couple more rooms, or, at least, more puzzles. Xx
Lindsay Davenport
Ok game.... Game was too short. Still enjoyed playing. Please fix other games though...dog house escape, cake room escape, escape the world, emi's magical escape 2 and 3, and joyful new year escape..please fix those.
janice hastings
Awesome The whole thing is awesome
Marcellious Robinson
Easy didn't like it as much.


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The man whose body was pulled Sunday afternoon from the Schuylkill River in Exeter Township has been identified.
James F. Hegedus, 65, of Exeter Township was pronounced dead at the scene near Gibraltar Road, said Berks County Assistant Chief Deputy Coroner Jonn M. Hollenbach.
An autopsy Monday at Reading Hospital determined he drowned, Hollenbach said. The manner of death was ruled a suicide.
Hegedus� body was recovered about one-half mile east of the Gibraltar Road Bridge over the river.
The body found by kayakers searching for Bradley Verret, a Kenhorst man, 26, last seen in the area of Trooper Thorn�s restaurant on Feb. 7.
Hollenbach said it did not appear that a missing person report had been filed for Hegedus.


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How gratifying it is when our nation's youth are celebrated for a change. If only they could reverse the growing stigma that is gradually being placed upon them and garner some well needed applause. At the Youth Awards, hosted last week, amongst all others was the highly anticipated Youth of Excellence or Youth of the Year Award. The nominees for this category were all commendable, but there was to be only one truly worthy of the accolade; Tevin Shepherd.
Tevin Shepherd is a national youth advocate and leader. Born and raised in Canaries, the 20-year-old has centered his life around community service and youth work. Here is his story.
How did it all start for you?
TS: It basically started from my community. I longed to see some sort of development in my area, to see the youth go forward and to make a positive impact on their lives. At what age did you get into community work? TS: I was between the ages of 14 and 15, and it began with a group called Project Can (which is short for Project Canaries). With them I engaged in a lot of community activities, and then I moved to the national level while supporting the local groups in my community. That then led to me becoming a member of the National Youth Council and then representing Saint Lucia through CARICOM youth forums. So it started from grassroots and now I'm here.
You've worked with the youth on all levels; how has the experience been for you?
TS: I always say that youth work has made me who I am. The rewards are not monetary, but you acquire new skills, you learn how to speak to others and work in teams. So it's personal development while helping develop others in the process, so it's a win-win situation. You begin to network, to meet amazing and well respected people so you get better opportunities in life. You were successful in obtaining the Youth of the Year Award.
How confident were you that you would win?
TS: I was very confident. Last year I was nominated for three categories including Youth of the Year, but I didn't win any. This year, I only got nominated for the Youth of Excellence Award and I won. My supporters and the people that I work with were confident and I guess this year they put me in that category to encompass all that I do.
Which of your achievements do you think helped win you the Youth of the Year Award?
TS: Well, in terms of my community, I teamed up with David Henry to organize the Under 14 Football Against Racism (FARE) programme. On a national level, I was a member of the Youth Month planning committee, where I assisted the planning for Youth Parliament, Youth Explosion and Youth Ecumenical service. Regionally, I was Saint Lucia's Youth Representative to the United Nations. Through their Development Programme, I worked with other young persons across CARICOM on initiatives such as the International Youth Federation Conference.
Explain your feelings when you received the Award.
TS: I was very humble; it was wonderful for me. And I wasn't only proud of myself but my community Canaries, as we got the award for best religious organization. So I was also proud of them because I wouldn't have been Youth of the Year without their support, being involved with them and getting the opportunity to develop them and contribute to their lives.
Do you do any work in your community singlehandedly?
TS: No. Everything I have done is in conjunction with the groups in the community; because that's what youth work is about. You cannot develop people without working with them. I have no personal projects. I may have initiated a project but I always work with people.
What is your opinion in terms of the level of youth development in your community, having worked with the youngsters of Canaries for the past five to six years?
TS: In Canaries I know some people are trying, and the challenge is getting people to come on board and to get involved. People want to see things happening before they decide to join the initiative. So the challenge is getting people to buy-in and to get on board. But I think we have a lot of people who are interested in the movement and who want to see themselves succeed and their community move forward. Tevin informed me that this Youth of the Year award is not to justify his work and to culminate his determined efforts in the community and across the island. He says that this award assures him that his deeds are appreciated and that he will continue his passionate endeavor of being a 'shepherd' for the youth.


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desktop | iPad | iPhone/iPod | iPhone 5 /iPod
Hope you will enjoy this new calendar with a warm cup of coffee/tea/chocolate! Have a beautiful November everyone! :)
"What is real is not the external form, but the essence of things… it is impossible for anyone to express anything essentially real by imitating its exterior surface."
~ Brancusi ~


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Its brushed metal frame and Gorilla Glass 4 screen might lend the Samsung Galaxy S®6 edge and S®6 an all-business look, but the phone doesn't skimp on the multimedia front. If music is your thing, this phone is game for anything from a personal serenade to a life-of-the-party concert. To up your game from ukelele to double-necked guitar, keep an ear out for just the right accessories.
1. Zone Out with Earphones
Samsung's Level Over headphones play nicely with Galaxy S6 Bluetooth connectivity, pumping out full, dynamic sound with hi-def 50mm drivers, natural-sounding Neodymium magnets and no wires to speak of. If you prefer to listen on the sly, go for a Samsung Level In in-ear headset. The balanced armature driver tweeters make acoustic guitars sing while the dynamic driver woofers transport you from the grocery store to the dance floor.
2. Fill Your Space
Some music demands a heightened presence, whether its a Berlioz opera or '60s psychedelia. Smart home theater systems, such as the Samsung HT-J5500W, balance sonic force with forward-thinking connectivity. In addition to 1,000 watts of Crystal Amplifier-powered clarity and 5.1-channel sound, this system syncs with your Galaxy S6 via the Samsung Shape app, putting multi-room speaker control and screen-sharing features in the palm of your hand (and making for one less remote to misplace).
3. Share the Music
Music brings us together, but it doesn't always wait for you to plan the perfect party. When you need to curate the vibe at the beach or barbecue, turn to a Samsung Level Box Mini with easy NFC pairing and up to 25 hours of playback on a full charge.
Photo Credits: Samsung
We Recommend
Samsung is a registered trademark of Samsung Electronics Co., Ltd. All other brands, products and services, and their respective trademarks, names and logos, are the property of their respective owners. All apps referenced, except where otherwise noted, are available in the Samsung GALAXY Apps store. For more information on any referenced apps, products or services, see the respective websites.
The above content is provided for entertainment and information purposes only. All information included herein is subject to change without notice. Samsung Electronics is not responsible for any direct or indirect damages, arising from or related to use or reliance of the above content.


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MP says Navy base push about long-term planning
A north Queensland MP says bringing part of the Navy to the region is a realistic goal for the next five years.
Senator Ian Macdonald is calling on the Prime Minister to move Sydney's naval base to Townsville or Cairns.
Herbert MP Ewan Jones says the idea is about long-term planning.
Mr Jones says it does not necessarily mean Sydney's base should be shut down.
"I don't think Ian is talking here about shutting down the Navy base in Sydney," he said.
"What he is talking about is if we are to develop the north of Australia it is not a three to five-year plan, it is for the next 200 years."
Topics: navy, cairns-4870, townsville-4810


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From WunderBlog (Bob Henson):
Data for July 2016 make it clear that this summer is worming its way into the nation's warmest batch on record, thanks in large part to consistently sultry nights in many areas. Meteorological summer so far–June plus July–has been the fifth warmest for the contiguous U.S. in 122 years of recordkeeping, according to the July climate report released on Thursday by NOAA's National Centers for Environmental Information (NCEI). Ahead of 2016 at this point are the scorching Dust Bowl summers of 1934 (#4) and 1936 (#1) along with the recent 2006 (#3) and 2012 (#2). The toasty summer so far is the result of the nation's warmest June on record followed by its 14th warmest July. Last month's warmth was focused across the nation's southern and eastern halves (see Figure 1), with New Mexico and Florida each recording their hottest July on record. Fourteen other states made it into their top ten warmest for July.
Warmest nights on record for June-July
It's the muggy nights that are imprinting themselves on the psyche of millions of Americans this summer. The average daily minimum for the contiguous U.S. was the warmest on record for June and July combined: 60.57°F, beating out 2015, 2010, 2002, and 2006. (See Figure 2.) Warmer nights are a hallmark of a climate being heated by added greenhouse gases, and it's long been recognized that nights should generally warm more than days, and winters more than summers, as climate change proceeds. Of the eight June-July periods with the warmest average daily temperatures (including both highs and lows), four are from Dust Bowl years. However, the eight years with the warmest average daily minimum temperatures are all from the 21st century. Urban heat islands are no doubt helping to increase overnight lows in large metropolitan areas; however, the nationwide extent of the trend toward warm nights goes well beyond this effect.
For the year to date through July, the U.S. has seen 15,061 daily record highs and just 2709 record daily lows, according to data compiled by meteorologist Guy Walton. In an email, Walton told me that "2016 is in a race with 2012 for that dubious distinction of having the highest ratio of record daily highs to record daily lows since 1920."
Wet to the north, dry to the south
After an unusually dry June across the contiguous U.S., July produced generous rains across much of the nation's northern tier, while leaving most of the Northeast and the nation's southern half on the dry side. It was the second-driest July on record for Georgia and the third-driest for Florida, with Wyoming and New Mexico also coming in among their top-ten driest. July was among the ten wettest on record in five states: Illinois, Kentucky, Minnesota, Missouri, and North Dakota. The weekly U.S. Drought Monitor issued on Thursday morning shows that more than 40% of California remains in extreme or exceptional drought, a situation that is unlikely to change before the 2016-17 wet season arrives (if then). Much of the state remains extremely vulnerable to fast-growing wildfire, especially over the next several months. Wildland fire potential is also expected to increase across the South this autumn, according to the latest outlooks from the National Interagency Coordination Center. (Torrential rains scattered over parts of the upper Gulf Coast this week will help tamp down the immediate drought and fire risk in those areas.)
The wet-north/dry-south tendency evident in July may be a foreshadowing of La Niña influence to come (see Figure 4 below). The strong El Niño of 2015-16 is giving way to borderline La Niña conditions, with the weekly index of sea-surface temperatures in the Niño3.4 region hanging near the -0.5°C threshold of La Niña over the past month. NOAA maintained a La Niña Watch in its monthly ENSO discussion issued Thursday, although the event is projected to be relatively weak if it does take shape. NOAA is giving a 55-60% chance of La Niña being present this fall and winter and a negligible chance of El Niño (below 10%). Because La Niña typically leads to a more consolidated jet stream, it often leaves southern parts of the 48 states on the dry side of upper-level flow as storm systems whip across the nation's heartland, keeping northern areas more moist.


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[slideshow id=56]
African-American alumni from the classes of 1980-1989 at local area high schools reunited Sunday in downtown Los Angeles at Club Tatou (formerly Prince's Grand Slam) to relive the early days of Los Angeles' hip-hop and dance crew scene and the Ultra Wave Party. Ultra Wave was a group of Los Angeles dance promoters who were often dubbed as the Westside version of Uncle Jam's Army, a hip-hop dance crew in the 1980s. Ultra Wave left their indelible mark on L.A.'s hip-hop scene with their unique dance styles, wardrobe, and dance crew battles at parties held for a time in Culver City at Veteran's Auditorium to rap, electro beat, nu-wave, ska, funk, R & B, and club music.
In the house that night included everyone from Egyptian Lover to legendary deejays Mark Luv, Chris Brazil, Vicious Lee, and General Lee. And the dance crews…off the chain!
Word had it, Ultra Wave is back as a monthly event and if that's the case, I think I know where my 32nd birthday is going down in October, lol.
For more information on the history of Ultra Wave and Sunday's party, head over to www.ultrawaveentertainment.com.
Big ups to my boy Gregory for bringing back the best damn party of all times! Ain't no party like an 80s party and California, yeah, we know how to party!
H/t: Hiltron Bailey for the photos!


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text:
Q&A With Morgan Lewis' Howard S. Beltzer
Beltzer has participated in recent significant Chapter 11 proceedings and multinational bankruptcies and restructurings, including the representation as co-counsel of Linens 'n Things Inc., the largest retailer to file in 2008.
Q: What is the most challenging case you've worked on, and why?
A: Perhaps the most challenging...
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text:
Discussion in 'UPS Discussions' started by grgrcr88, Dec 23, 2009.
Ha, gotcha!! Lets see everyones Christmas trees this holiday season!!
I don't put up a tree. I do have a wreath on the front door and tape the Christmas cards on the wall in my living room but that is the extent of my decorations.
My grandson 9 yrs old and I made this Christmas tree out of stuff in the pool house. A towel stand, a floating basketball hoop and lots of pool noodles.
It was fun and we enjoyed ourselves in good quality time spent together.
grgr......Beautiful tree!
Here's hubby with our Christmas tree decorated with apples........
That's one creative and interesting tree Hoax.
Aren't those Webkins perching in the tree?
My daughter has quite a few of those.
More, I hate to rat anyone out, but I SAW this guy hanging out with a redhead at a bookstore!
Just thought you should know......
I know.....He's a wild & crazy guy !!
my tree !!!
Alright, alright, I'll show you mine!
Looks better in real life....!
Kooks good Ovah.
I can almost smell it through the screen.
I think all the trees look better "in person" because the lights don't come across so well. It's like trying to photograph your outside Christmas lights...they never look as good as what you see.
Over....your tree is real and probably smells great. Mine is fake and I spray a pine scented room spray to try to capture the smell.
Thanks, Mr Jingles! And welcome to Brown Cafe.
No tree and no decorations at my house. When your kids are up and grown then you don't have to do it any more so I don't!
Our family tree. Merry Christmas everyone!
(Hope this works. I couldn't find any directions on the proper way to upload a photo)
Nice tree! What's hanging from the ceiling?
(Don't you hate people who analyze everything in your picture???)
Looks like some sort of candelabra or somethin'. No wait a minute, I see what you're talking about. That thing over the grand piano, right?
That's the tassle pull chain end for the ceiling fan.
(Thank's Hoax' for fixing my photo.)
Uhhhh....what exactly are you looking at????
Separate names with a comma.


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Rumours of on-premises software's demise greatly exaggerated
Microsoft commits to another version of SharePoint for your bit barn
Microsoft has let it be known that it will create another version of SharePoint, and release a version of it for on-premises operations.
"We know that the move to cloud doesn't happen all at once," Microsoft writes , adding "While we've seen growing demand for SharePoint Online, we recognize that our customers have a range of requirements that make maintaining existing SharePoint Server deployments the right decision for some."
Wherever it runs, Microsoft promises SharePoint will offer a new feature called "Delve" that sounds like an enhanced search facility. There's also an enhanced Portals offering in the works, the first of which will focus on knowledge management. Redmond's also flagging more APIs, so that SharePoint can suck it and see with the rest of Office365 and plenty of other data sources and destinations.
There's also a statement of intention that this will all be delivered in a hybrid model, a non-surprise of a position given Office365 is herding productivity suite user to the cloud.
It's no surprise that Microsoft's persisting with on-premises SharePoint, as many users have lots of data – often large files - managed by the application. Going cloud-only would likely make for an inferior user experience for those sucking down 20MB files a few times a day.
Microsoft is, of course, keen to turn users into locked-in subscribers of as many services as possible, rather than mere licensees who can front up every two years to ask sir for less. Hybridising SharePoint means Microsoft has a chance to pull that off while also retaining its bit barn presence, a neat double act if ever there was one. ®
Bootnote: First commentard to figure out the image is clever.
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London, May 21 (ANI): British tennis player Andy Murray, a Scot by birth, has said that he wouldn't mind his kids having English accents.
"I don't think it would bother me at all if my kids grew up with English accents. A lot of my best friends are English and I've spent a lot of time here. My home is in London now and I love living here. I'm sure it would be different if my girlfriend Kim was Scottish - but she is not," The Sun quoted Murray, as saying.
Murray also said that he would take a game of dominoes with his grandmother as seriously as a Grand Slam tennis final against Rafa Nadal. (ANI)


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You can trace the deregulation process in financial markets as far back as 1986 after so called 'big bang'. London capital markets prior to 'big bang' were non competitive in the increasingly global market. Restrictions and fail safe mechanisms trusted for centuries were swept away in the manic pursuit of profit.
It's interesting to note that the few European countries that did not take part in this deregulation to the same extent have since suffered least.
In the US fundamental and pragmatic banking regulations, which arose from the devastating financial collapses of the Great Depression, for decades strengthened U.S. banks and capital markets, making them the twin engines of American growth and the envy of the world. They were gradually removed in the decades after 'big bang' and finally abolished altogether by the Securities and Exchange commission in 2004.
I maintain that these moves contributed in principal to the eventual debacle we are mired in right now. A new breed of investment banker was born that lacked the traditional caution and restraint of history. Institutions such as pensions funds and insurance companies needed to produce ever increasing returns from investment to satisfy their own shareholders.
The growth this new order created over decades was truly astonishing yet it was in reality nothing more than a giant Ponsi scheme doomed to eventual collapse.


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I am sitting on a couple months of raw photos and video and an incredible couple of stories and blogs from this summers foray into the Wind River Range and then City of Rocks in Idaho.
The only problem is, my life is a series of plane flights and frantic back and forths–fragmented into cardboard boxes, backpacks and bags. That doesn't lend itself to processing and rolling out media. Hell, just managing my blood sugar day to day on the road–stressed out from being so disorganized–is a monumental task.
This dichotomy has been fueled by the fact that I am still embedded in Utah while Stefanie and the baby are still embedded in Boston.
Trying to split time has been too much effort and it's not good for us personally, nor is it good for LivingVertical. So we are refocusing and rolling with the punches in order to keep moving forward, not straightforward with type1 diabetes–because we have a lot of exciting developments, media, projects and events that are in the hopper for the upcoming year.
Long story short, I am heading back to the east coast to consolidate the family and take the next steps with LV from that point. As we refocus, here are some of the next steps under consideration: Maximizing the exposure of the Project365 documentary, making
team LivingVertical more of an adventure based diabetes community than a distinct "team", diabetic climbing events/trips, SweetestSummit programs, publishing a book, selling photo prints and tutorial/educational blogs and YouTube releases–as well as the creation of an awesome short film of the Wind River Project and lots of blogs and photos! These times of transition are important because they help us figure out what's working and what's not–and where our heart really is moving forward.
See something you like in this list? Tell us about it! Don't see what you'd like here? Tell us!


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I was treated to lunch this afternoon as a belated birthday present. We tried Ethiopian food at Ethiopian Love restaurant in Chinatown.
Based on reviews, we tried the vegetarian sampler, sambussas, and my carnivore friends had a lamb dish. The sampler plate came with Misir Wot, a spicy red lentil stew, Kik, a yellow split pea stew, and potatoes.
The sambussas were similar to samosas, with a filling of cooked lentils. It was served with a mildly spicy sauce made with red wine, spices, and chilies.
All of that was served with injera, a flatbread made of fermented teff traditionally, which is a grass-like grain. Their version was made of a mixture of teff and wheat.
Injera is sour and spongy, like a flat pancake. The tanginess goes well with the other food.
When I got home, I was so inspired, and I had all the ingredients I would need, that I attempted a copycat recipe of the Misir Wot. Mine is definitely not as dark or as richly spiced as the one we ate, but taste-wise, it comes pretty close. I'll play with the recipe for now, but until then, this one is still a tasty dish.
Red lentils are tiny and cook up in a flash. Most Ethiopian recipes call for Berbere spice, which is usually pretty hot, but I used the spices you'd find and added them separately, so I could control the heat. This is a mild version. Adjust the cayenne to your taste.
The list of ingredients is long, but this is just a little more than a dump-and-boil dish. You can serve it with my fake injera, which isn't fermented. It cheats, using lemon juice to give it tang. Here's a link to my fake injera recipe (which is also on this site.)
Vegan Recipe: Misir Wot
Spice Paste: (all spices are ground, dried)
- 1 onion, chopped
- 4 cloves garlic, chopped
- 1 TABLESPOON minced fresh ginger
- 4-1/2 teaspoons paprika
- 2 teaspoons cumin
- 1/2 teaspoon coriander
- 1/2 teaspoon cardamom
- 1/2 teaspoon turmeric
- 1/2 teaspoon fenugreek (you can use whole or ground)
- 1/4 teaspoon cloves
- 1/4 teaspoon allspice
- 1/4 teaspoon ginger
- 1/4 teaspoon cinnamon
- 1/2 teaspoon cayenne
- 1/2 cup water
- 3 TABLESPOONS oil
- 1 pound red lentils (2-1/2 cups)
- 5 cups water
- 1 can (14.5 ounces, 411 grams) diced tomatoes
- 2 teaspoons salt
- 5 TABLESPOONS red wine vinegar
Place all the spice paste ingredients in a blender to make the spice paste. Stop the blender often and scrape down the sides. Blend until you have a relatively smooth paste.
In a large pot on medium-high heat, cook spice paste in oil 5 minutes, stirring often so you don't burn it.
Add lentils, water, tomatoes, salt.
Cover, bring to a boil. Cook, stirring often, until lentils are done and you get a thick stew, about 20-30 minutes. Stir more often near the end of the cooking period, scraping the bottom of the pot, so the stew does not scorch.
Stir in vinegar.
Be careful at the end, because the stew will spit hot gobs of burning stew at you. Once it's done, pull it from the heat and keep it covered until it stops spitting.


text: BAH Share: Name: BAH Date: March 21, 2018 Time: 5:30 PM - 7:00 PM MDT Website: http://www.palisadecoc.com Event Description: BAH @ Palisade Fire Station presented by the Town of Palisade and partnered with Pattie's Tiny Cakes To Current Calendar Location: 341 W 7th Street, Palisade CO Date/Time Information: 5:30-7:00 PM Contact Information: Palisade Chamber of Commerce 970-464-7458 Send an Email Fees/Admission: $6 RSVP or $8 at the door. Set a Reminder: Enter your email address below to receive a reminder message. -- Select Days Before Event -- 1 Day 2 Days 3 Days 1 Week To Current Calendar Business Directory News Releases Events Calendar Hot Deals Member To Member Deals Job Postings Contact Us Information & Brochures Weather Join The Chamber


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- Calories 804
- Fat 43g
- Sat fat 9g
- Cholesterol 36mg
- Sodium 1,002mg
- Protein 34g
- Carbohydrate 71g
- Sugar 2g
- Fiber 7g
- Iron 7mg
- Calcium 308mg
Pecan Pesto Shells With Sausage
Marcus Nilsson
How to Make It
Step 1
Cook the pasta according to the package directions, reserving ½ cup of the cooking water; drain the pasta and return it to the pot.
Step 2
Pulse together the parsley, pecans, Parmesan, ½ teaspoon salt, and ¼ teaspoon pepper in a food processor until finely chopped. With the machine running, add 5 tablespoons of the oil and process until smooth.
Step 3
Heat the remaining tablespoon of oil in a large skillet over medium-high heat. Add the sausage and cook, breaking it up with a spoon, until browned, 6 to 8 minutes.
Step 4
Add the pesto, sausage, and ¼ cup of the reserved cooking water to the pasta and toss to coat (adding more cooking water as needed to loosen the sauce). Sprinkle with Parmesan.