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Fight All California Traffic Tickets
- Get It Dismissed Or 100% Money Back
- No Court Appearance Necessary
- No Hidden Fees Whatsoever
- Get It Done Fast & Easy / Risk Free
CA Cell Phone Ticket
- Get It Dismissed Or 100% Money Back
- No Court Appearance Necessary
- No Hidden Fees Whatsoever
- Get It Done Fast & Easy / Risk Free
Are Red Light Camera Tickets Legal?
After people get over the initial surprise and the anger that follows the opening of an envelope containing a red light camera ticket the first question that comes to mind is: ARE RED LIGHT CAMERA TICKETS LEGAL?
In California, automated red light camera tickets are legal. Basically, any jurisdiction may choose to use automated red light camera enforcement system as long as certain rules are followed. Sections 21455.5 vc , 21455.6 vc and 21455.7 vc govern the rules for the red light camera enforcement systems.
Some of the rules for the red light camera systems have been changes to make the presence of the enforcement system more visible to the public so that people are aware of the system in place. Once such requirement is the placement of red light camera enforcement signs within 200 feet of an intersection.
There are also rules and requirements regarding the minimum yellow clearance time at an intersection. The automated to make red light camera tickets legal the operating agencies must ensure that the minimum yellow clearance time is adequate to allow a driver to safely come to a stop when traveling at the prevailing speed of the roadway.
There are also many other requirements that the cities and the counties are supposed to follow in order to issue red light camera tickets legally.
If you were have a red light camera ticket and you would like to contest it using our service, you can sign up by clicking on the red button on this page and completing our simple sign up process. Then, just fax us a copy of your red light ticket.
Remember red light camera tickets legal or not carry a hefty fine of approximately $500. If you choose to throw in the towel instead of fighting it you will have to pay the red light ticket fine. Also, even if you decide to do traffic school to keep the points off your DMV record you would still have to pay the fine and the traffic school fee to the court and the traffic school itself.
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B.H. Question
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August 15th, 2006, 10:20 AM
Super Mommy
Join Date: Sep 2005
Posts: 653
I know ther has probably been a million questions about this but....
I am 33 weeks pregnant right now, the past week and a half I have been having serious B.H. Strong ones at that, and sometimes with pressure. They do tend to go away when I sit and relax.
My question is.. would I have to be worried about dialating already? Is this something to be concerned about. What would be the % of me having an early labor?
If anyone knows it would be helpful thankyou.
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According to a new Yahoo! study, 56% of people say they share information via social networks like Facebook and Twitter on a daily basis or even more frequently.
According to Y!'s Global Product Insights team and Brand and Consumer Marketing insights team that conducted the study, ""Power Sharers" tend to be younger, male, and more liberal (both socially and politically). Although they're a minority (they make up 15% of all social network users), they tend to be more passionate, aggressive and opinionated in how they use social networking sites," informs Yahoo!.
In addition to the typical sharing motivations (wanting to share content that's entertaining, informative and helps people express themselves), Power Sharers gravitate toward more controversial content. Yahoo!'s researchers found that they're motivated by the need to start and develop provocative digital dialogues.
Check the infographic below:
[Source:Yahoo Advertising blog]


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Mediaeval Miniatures and Illuminations, Catalogue No. 169, Indo Persian Paintings
1960 · New York
New York: Philip C. Duschnes, 1960. Very Good. Very good in wrappers. Pamphlet lightly rubbed at spine ends and corners. Please Note: This book has been transferred to Between the Covers from another database and might not be described to our usual standards. Please inquire for more detailed condition information. (Inventory #: 143459)


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NO SILENT JUSTICE Gorsuch breaks mold, asks numerous questions in Supreme Court debut
by Fox News
WASHINGTON – An upbeat Justice Neil Gorsuch wasted little time getting to work in his first public session Monday as the 113th member of the Supreme Court.
Sitting at the far right end of the nine-member bench, Gorsuch spent the morning hearing three oral arguments, each lasting about an hour. In his first case, considering a federal workplace discrimination claim, the newest justice was among the most active of questioners -- unusual for the court "rookie."
At the start of the morning session, Chief Justice John Roberts publicly acknowledged his new colleague in the crowded courtroom, wishing him a "long and happy career in our common calling."
Gorsuch responded by thanking the other justices for giving him a "warm welcome."
The 49-year-old Colorado native paid close attention to the arguments, sitting straight up and resting his hand occasionally on his chin.
He remained focused -- not even chatting with his "bench neighbor," Justice Sonia Sotomayor -- as he asked a number of questions of counsel. The back-and-forth exchanges lasted more than 10 minutes of the first 60-minute argument.
The first case out of the gate for Gorsuch was not a blockbuster, but the justice repeatedly pressed lawyers from both sides with his positions.
When one attorney admitted he tended to agree with the justice on one point, Gorsuch dryly replied, "I hope so."
At one point, he even apologized for the amount of questions, saying, "Sorry for taking up so much time."
The other cases being argued separately Monday deal with a property rights dispute and securities class-action lawsuits.
Settling In
Even before Monday's arguments, Gorsuch had begun settling in at the court, arranging his chambers to create a comfortable, efficient workplace. Reminders of his roots in Colorado and the West will grace his offices, along with plenty of photos of his family and friends.
He is allowed to hire secretaries, a messenger, and four law clerks -- who typically serve for one year.
Those clerks will be especially important helping the justice get up to speed on his caseload, since joining the court in the midst of the term is not standard. It will be a nonstop whirl of activity until the term effectively ends in late June.
All four of the law clerks brought on in recent days served previously for then-Judge Gorsuch, and are all experienced litigators or academics. Two of them later went on to clerk for Antonin Scalia (the late justice whose seat Gorsuch is now occupying) and Justice Sonia Sotomayor.
His colleagues are welcoming their newest member.
"We hope we're serving with Justice Gorsuch for the next 25 years," Roberts said last week before a university audience in New York. "It's kind of like a marriage. If you're going to be with someone that long, you can't have knock-down, drag-out fights over a case."
Lunch Is Served
Food for thought for the newest member of the Supreme Court: being the junior justice has its benefits and challenges.
For Gorsuch, it will mean being assigned to the court's internal Cafeteria Committee, where dessert toppings and silverware choices will compete for his time with constitutional issues big and small -- all part of the dizzying first few weeks for the justice.
Justice Elena Kagan, who had been the court "newbie" since 2010, unwittingly gave her future colleague some personal advice on managing the job. She appeared last September at a Colorado legal conference with Gorsuch and spoke to what it was like to have the least seniority.
"I think this is a way to kind of humble people," she said about her stint as one of the office lunch monitors. "You think you're kind of hot stuff. You're an important person. You've just been confirmed to the United States Supreme Court. And now you are going to monthly cafeteria committee meetings where literally the agenda is what happened to the good recipe for the chocolate chip cookies."
And the rookie hears about it when the food doesn't rate. One tradition of the court is the justices eat together privately after oral arguments.
"Somebody will say, 'Who's our representative to the cafeteria committee again?'" she told Gorsuch. "Like they don't know, right? And then they'll say, 'This soup is very salty.' And I'm like supposed to go fix it myself?"
Kagan recalled her proudest moment was getting a frozen yogurt machine installed in the dining area, which is open to the public.
She had been on the internal committee for seven years, with Justice Stephen Breyer in the job 11 years before that.
"It's a way of bringing them back down to Earth after the excitement of confirmation and appointment," Roberts said in 2011. Roberts' role as "first among equals," though, meant he never had to endure any of the "new guy" responsibilities.
Another duty for the "junior" justice is to answer the door when the members meet privately for their weekly closed-door conferences -- voting on cases and deciding which petitions get added to the docket. His first such conference will be this Thursday.
Gorsuch will also take notes at the conferences, and will vote last when cases get decided.
It is a learning curve that many on the court admit can be baffling and often overwhelming.
Justice Samuel Alito said he frequently got lost in the marbled halls of the court when he joined in 2006, especially since the building was undergoing a massive internal renovation at the time.
Breyer said it took him years to feel fully comfortable in the job.
And Justice Clarence Thomas recalled what Justice Byron White told him when he donned the robes in 1991. White, whose clerks included Gorsuch, said, "Well, Clarence, in your first five years you wonder how you got here. After that you wonder how your colleagues got here."
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Daniel Stalder, Trinh Cung, Steffen Gloekler1, Pascal Meier1, Evelyn Schlappritzi, André Haeberli, Santica Marcovina2, Christian Seiler1, Manfred Heller*
Laboratory of Thrombosis Research, Department of Clinical Research, University of Bern, 3010 Bern, Switzerland
1Department of Cardiology, University Hospital, Bern, Switzerland
2Northwest Lipid Metabolism and Diabetes Research Laboratory, University of Washington, Seattle, WA, USA
Received Date: August 20, 2008; Accepted Date: October 17, 2008; Published Date: November 05, 2008
Citation: Stalder D, Cung T, Gloekler S, Meier P, Schlappritzi E, et al. (2008) Apolipoprotein(a) Size and Lipoprotein(a) Concentrations in Patients with Good and Poor Coronary Collateral Flow-an Interrelation Discovered by Proteomic Screening of Pooled Plasma Samples. J Proteomics Bioinform 1: 389-400. doi: 10.4172/jpb.1000048
Copyright: © 2008 Stalder D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Visit for more related articles at Journal of Proteomics & Bioinformatics
We discovered and validated medium sized
(a) as a marker for good myocardial collaterization. A total of 80 subjects were investigated in two serial studies: a discovery study (n=14) applying a pooling strategy to a gel and label free proteomics platform followed by a validation study (n=80) measuring apolipoprotein(a) isoforms and concentration in individual subjects. Degree of myocardial collaterization as well as apolipoprotein(a) concentration and isoform determination were performed by state-of-the-art methodologies. As apolipoprotein(a) concentration negatively correlates with isoform size (variable number of Kringle-IV type 2 repeats in human population), subjects were grouped into patients with small, medium and large apolipoprotein(a) isoforms for the statistical analysis. Among the 70 subjects with medium and large apolipoprotein(a) isoforms (>17 Kringle-IV type 2 repeats), subjects with insufficient collaterization (n=57) had a median apolipoprotein(a) concentration of 11.9 nmol/L, while patients with sufficient collaterization (n=13) had a median concentration of 31.3 nmol/L (p=0.033, Mann-Whitney U-test). Among the 52 subjects with medium sized apolipoprotein(a) isoforms (30< Kringle IV type 2 repeats >17) the difference in concentration was even more significant (13.4 vs 33.5 nmol/L, p=0.008).
%RSD: Relative Standard Deviation; apo(a): Apolipoprotein(a); CAD: Coronary Artery Disease; CFI: Collateral Flow Index; CID: Collision-induced Dissociation; FWHM: Full Width at Half Maximum (peak intensity); GlcNAc: N-acetylglucosamine; HCII: Heparin Cofactor II; IEF: Isoelectric Focusing; IPG: Immobilized pH Gradient; LACB: Beta-lactoglobulin; Lp(a): Lipoprotein(a); OGE: Off GelTM Electrophoresis; PMSS: Peptide Match Score Summation; RP-HPLC: Reversed Phase HPLC
Blood vessels connecting left and right coronary arteries are referred to as "coronary collateral vessels" or "coronary anastomoses". In the presence of obstructive or occlusive coronary artery disease, anastomoses between different vascular regions may serve as natural bypass for blood to reach myocardial territories distal to an arterial obstruction or occlusion, thereby preventing or mitigating myocardial infarction. The therapeutic promotion of collaterals would offer an alternative treatment strategy for coronary artery disease (CAD) patients. Today, the presence of sufficient collaterals to prevent ischemia during coronary occlusion can only be determined by invasive methods. The collateral flow index (CFI) determination, using ultra thin Doppler and pressure angioplasty guide wires, is currently the only quantitative method for the determination of coronary collateral flow in human patients. It has been determined empirically that a CFI 0.25 is sufficient to prevent myocardial ischemia in the event of a one-minute coronary occlusion (Pohl et al., 2001). Furthermore it has been shown that sufficient collateral arterioles occur more frequently in the presence of stenotic lesions in coronary arteries (Urban et al., 1987; Wustmann et al., 2003). However the response of collateral development to a certain obstructive stimulus (higher flow rates and high shear stress in preexisting interconnecting arterioles) is highly variable and it is adequate only in about 1/3 of patients with CAD to prevent myocardial ischemia during occlusion (Pohl et al., 2001). Both, angiogenesis and arteriogenesis are involved in enhancing the degree of collaterization. The latter is thought of being the more important factor, as increasing the diameters of already existing arterioles contributes more to the blood supply than the formation of new capillaries. That is why any supporting remodeling agent or therapy which improves revascularization is of great interest. Most insight on the molecular biology of arteriogenesis in context with collateral growth has come from animal models. In short, increased shear stress in preexisting arterioles induces inflammation of the endothelium. The attracted mononuclear blood cells together with the endothelium then orchestrate the remodeling process, which includes cell proliferation, degradation and rebuilding of extra cellular matrix (Heil and Schaper, 2007).
Current therapeutic approaches to obstructive CAD include pharmacologic reduction of myocardial oxygen demand and mechanical restoration of blood flow to regions lacking sufficient supply. No agents stimulating arteriogenesis or angiogenesis are currently used in therapy, although some studies indicate that certain angiogenic factors do enhance collateral flow in the short term (Seiler et al., 2001; Zbinden et al., 2005), but are not applicable because most angiogenic factors are also atherogenic. Recently, a clinical study testing a 3-month physical endurance exercise program has shown to augment coronary collateral supply to healthy and stenotic coronary arteries (Zbinden et al., 2007).
In our study we searched for plasma protein biomarkers correlating with the degree of myocardial collaterization by comparing pooled plasma samples of patients with very well developed collaterals (CFI>0.25) to patients with very badly developed collaterals (CFI<0.1). The comparison was performed with our recently developed gel and label free proteomic screening platform (Stalder et al., 2008) employing immunodepletion of the twelve most abundant plasma proteins followed by protein fractionation prior to LC-MS/ MS based protein identification of the tryptically digested fractions. We used the probability based peptide match score summation (PMSS) as relative protein abundance indicator, an approach first shown by Allet and Colinge (Allet et al., 2004; Colinge et al., 2005) and adapted in our laboratory (Heller et al., 2007; Stalder et al., 2008). PMSS is based on spectrum sampling (Liu et al., 2004), and is based on the assumption that the frequency of detecting peptides of a certain protein correlates with its relative concentration. While spectrum counting counts the frequency of identified peptides, PMSS uses the quality (z-score) of the peptide identification as a weighting factor. The finding of differential apo(a) expression discovered with the proteomics study was then successfully validated by measuring specific protein concentrations in all 80 subjects.
Patient Samples
Patients undergoing coronary angiography because of chest pain or a positive treadmill exercise test were enrolled for this study after written consent of the patients and with approval of the local ethical committee. Blood samples of 80 patients (age 61±11 years, 15 women) referred for diagnostic coronary angiography and undergoing invasive collateral assessment were drawn into EDTA containing tubes. Clinical parameters of all subjects are given in Appendix C and no statistical significance was apparent between the two groups with sufficient or non-sufficient collateralization based on a two-tailed students t-test assuming equal variance. After blood cells were depleted by centrifugation at 2,000 g (20°C) for 20 minutes, protease inhibitor cocktail CompleteTM from Roche was added according to manufacturers recommendations. Aliquots of 250μL and 1 mL were stored at -80°C. After all samples were collected, seven aliquots of patients with very high and seven patients with very low collateral flow index (CFI>0.25: Pool A, CFI<0.1: Pool B) were thawed at 37°C for 5 minutes, pooled into two pools, divided into 100 μL aliquots and stored at -80°C.
Assessment of Collateral Flow
Invasive assessment of coronary collateral flow index was performed by simultaneous measurement of distal coronary occlusive pressure (Poccl), aortic pressure (Pao) and central venous pressure (CVP) during a 1-minute coronary balloon occlusion. CFI was calculated with the following formula: CFI=( Poccl-CVP)/( Pao-CVP) (Seiler et al., 1998).
Fractionation of Plasma Proteins
Plasma pool samples (100 μL) were thawed at 37°C for 5 minutes and 32 μL of plasma was depleted of the 12 most abundant plasma proteins (Albumin, IgG, Transferrin, Haptoglobin, Alpha-1-Antitrypsin, Alpha-2-Macroglobulin, IgA, IgM, Orosomucoid, ApoA-I, ApoA-II, Fibrinogen) through immunodepletion using SepproTM IgY-12 depletion kit (GenWay, San Diego, CA, USA) according to the provided standard protocol. Depleted plasma proteins (~250 μg) were immediately fractionated into 51 fractions according to their hydrophobicity by RP-HPLC on a Summit HPLC System (Dionex, Idstein, Germany) using a GRACE VIDAC C4 column (4.5 mm i.d. x 150 mm length), which was kept at 58°C, and a Foxy fraction collector under the control of Chromeleon Software (Dionex, Idstein, Germany). The samples were acidified by addition of TFA to the final concentration of 0.1% (v/v), incubated, centrifuged and loaded onto the column by two consecutive injections of 200 microL at a flow rate of 0.75 mL/min of solvent A (0.1% TFA in water/acetonitrile 97:3). Columns were developed by a sigmoid acetonitrile gradient of 3 to 100% solvent B (0.1% TFA in water/acetonitrile 3:97) in 54 min at a flow rate of 0.75 mL/min. Fractions of 0.75 mL were collected each minute. The UV trace at 214 nm and 280 nm as well as the back pressure of the column was recorded by the software. Fractions were collected in 1.5 mL Eppendorf tubes which were closed, labeled, and pierced in the cap with a needle before storage at -80°C. The frozen fractions were then lyophilized overnight in a Christ Alpha 2-4 LSC freeze dryer (Kühner AG, Birsfelden, Switzerland). The lyophilized protein fractions were kept at -20°C until further use.
Protein Digestion, LC-MS/MS and Protein Identification
Sequencing grade modified trypsin was obtained from Promega (Catalys, Wallisellen, Switzerland). Formic acid, LC-MS grade acetonitrile, Tris buffer and ammonium bicarbonate, were purchased from Fluka (Buchs, Switzerland). Lyophilized RP-HPLC samples were reconstituted and digested by adding 14 microL of 0.1 M Tris pH 8, 5 mM DTT, 50 ng/microL trypsin and snapping the tube with the index finger, in order to dissolve all of the lyophilized proteins. The liquid was spun down and incubated overnight at room temperature. In order to maximize reproducibility, proteins were not alkylated prior to digestion, but constantly kept in a reducing environment. The digestions were stopped by adding 2 microL of concentrated formic acid. Digests were analyzed by LC-MS/MS by loading 95% of the sample on a homemade 0.15 x 50 mm C8 RP column, applying a 40-minutes acetonitrile gradient on an Esquire3000 ion trap mass spectrometer (Bruker Daltonics, Bremen, Germany) essentially as described elsewhere (Heller et al., 2007).
Peak lists from the raw data were created by Data Analysis version 3.1 (Bruker Daltonics, Bremen, Germany) using the following parameters. MS/MS compounds exceeding a total ion chromatogram intensity of 4000 ion counts were exported and all spectra from the same precursor eluting within a retention time window of 0.5 minutes were compiled to one MS/MS peak list. MS/MS peak detection was made with the Apex peak finder algorithm using a peak width at half maximum (FWHM) of 0.1 m/z, a signal-to-noise ratio (S/N) of one, a relative to base peak intensity threshold of 2%, and an absolute intensity threshold of 10 ion counts as parameters. A mixed list of deconvoluted and nondeconvoluted MS and MS/MS signals, with an allowance for only the 200 most abundant peaks from non-deconvoluted MS/MS signals of each spectrum, were exported into Mascot generic file format text (mgf) files. MS signal deconvolution was set to "Auto" for resolved isotope, and a maximum charge of four with minimally three peaks in set and a molecular weight agreement of 0.05% for related ion deconvolution, respectively. MS/MS peak deconvolutions were allowed for a maximum charge of one only. S/N and FWHM values were also exported into the mgf files.
CID spectra interpretation was performed with the PHENYX v2.1 search engine on the vital-it.ch server operated by GeneBio (Geneva, Switzerland) against the latest human UniProt-SwissProt 51.0 (295981 individual entries) protein database allowing variable modifications of Met oxidation, Asn/Gln deamidation and formylation of free amino groups. Parent and fragment mass tolerances were set to 2 and 0.8 Da. Up to two missed cleavages and half tryptic peptides were allowed. Peptide identifications with z-scores>8 were accepted, but reconsidered if a protein identification was based on a single peptide. Each CID spectrum was allocated to only one peptide identification, thus preventing identification redundancy.
Protein Identification Acceptance Criteria
False positive rates (FPR) were determined by applying five representative vivid peak lists from different regions of the fractionations to the human UniProt-SwissProt 51.0 (295981 individual entries) database and to the reversed decoy database of equal size. The FPR are shown in appendix A. A FPR of zero was observed with z-scores above 9.2, which corresponds to p-value <1016. A false positive rate of less than 3% was observed at z-scores >8. Thus protein identifications were automatically accepted when at least two different peptides with z-scores >8 were found (FPR: 0.032). Protein identifications were considered reproduced when found in identical fractions across all runs. A shift of one RP-HPLC fraction was tolerated.
Data Processing and Peptide Match Score Summation (PMSS)
Several Perl scripts were developed and used in this study. They are available upon request from the authors. We hereby offer a short description of the dataflow and the involved scripts:CID spectra interpretation was performed on the PHENYX web server, which is based on the probabilistic OLAV scoring algorithm (Colinge et al., 2003; Magnin et al., 2004; Colinge et al., 2004). The resulting xml files (one result file per protein fraction) containing peptide identifications and their probabilistic identification scores, were downloaded from the server. Semi quantitative protein abundance scores and abundance distributions were calculated within individual fractions and the complete analysis by summing peptide match scores (z-scores >7), a technique called peptide match score summation (PMSS) (Colinge et al ., 2005; Heller et al ., 2007). This resulted in comparable PMSS based protein abundance scores and abundance distribution patterns for every protein identification. Some details: In case a peptide could be assigned to two different protein identifications, the score of the peptide was only added to the one with the higher sequence coverage. If a peptide was identified several times, all z-scores were added to the PMSS score, as long as it was derived from a different MS/MS spectrum. Peptide identification acceptance criteria (zscores>8) were more stringent than criteria allowing peptides to contribute to PMSS based abundance scores (zscores>7). The Phenyx z-score is a true probabilistic score without bias by peptide length or charge. Z-score based PMSS abundance representation therefore correlates extremely well with the spectrum sampling approach (Colinge et al ., 2005; Heller et a ., 2007).
Apolipoprotein(a) Size and Molar Lp(a) Concentration Determinations
The levels of Lp(a) in plasma were measured in all 80 subjects by a double monoclonal antibody-based ELISA. The detecting monoclonal antibody in this assay is specific to a unique epitope in apo(a), namely Kringle 4 (K4) type 9 and therefore, the accuracy of the assay is not affected by apo(a) size polymorphism (Marcovina et a l., 1995). Lp(a) concentrations were reported in nmol/L. The apo(a) isoform size was determined in all 80 subjects by high-resolution sodium dodecyl sulfate-agarose gel electrophoreses followed by immunoblotting as previously reported (Marcovina et al., 1993). Briefly, individuals with two apo(a) isoforms well defined in the gel were immediately assigned the relative kringle number to each isoform. Analysis of all samples with only one apparent isoform were repeated using an increased amount of plasma to verify whether or not it was possible to detect a second isoform present in a very small amount. Samples with no clear separation between two closely migrating bands were also reanalyzed. It has been determined that the logarithm of the number of K4 obtained by genotyping is highly correlated with the mobility of the isoforms on agarose gel (Marcovina et al., 1996b). Therefore, the apo(a) isoforms are designated by the relative number of Kringle 4 type 2 domains.
Statistical Analysis
For the relative protein abundance assessment from the proteomics screen, a random ratio analysis (Stalder et al., 2008) using PMSS values of protein identification was performed. Proteins standing out from the normal noise level were considered as potential biomarkers for myocardial collateralization (Table 1). Due to reasons of availability only concentration levels of apo(a) was chosen for validation and measured in all 80 patient samples by immuno-affinity methods and tested for significant differences in median protein concentrations between patients with sufficient and insufficient collateral flow index by the non-parametrical Mann-Whitney U-test.
Proteome Screening of Plasma Pools
The comparative protein profile analysis of the two pools was performed twice, which allowed for a rough estimation of the reproducibility of the comparison. Furthermore the two pooled samples were compared in a preliminary analysis using a different RP-HPLC column (mRP-C18, Agilent Technologies, Waldbronn, Germany). The results are not shown, but were used to confirm the results from the presented screen. The PMSS based abundance profiles of protein identifications were well reproduced in the four runs, with abundance profiles from replicates matching better than the ones from differing pool samples (Figure 1). In the four runs a total of 1601 different peptides were identified, whereof 1505 possessed one or more sibling peptide describing the same parent protein. In total, 121 different proteins were identified by at least two non-redundant peptides.
Figure 1: PMSS profiles of proteins identified in all four samples. This figure demonstrates the high reproducibility of the profiling platform and gives an impression over the fractionation power of the system. Each line on the y-axis represents an identified protein of a non redundant protein list, which was sorted by fraction of maximal identification, whereas white pixels stand for high PMSS scores.
The additional 94 one peptide protein identifications (all with a z-score >8) were not used for the screen, but might still be of interest with respect to the characterization of the human plasma proteome. From the 121 different protein identifications based on two or more peptides, 84 identifications were found in all four runs and nine identifications were only found with pool A (CFI>0.25), six proteins were found solely in pool B (CFI<0.1) and eight identifications were not reproduced in pool A or pool B, despite possessing two peptide identifications (Appendix B). Furthermore fourteen protein identifications could be accredited to keratin and IgG chains. A non-redundant list of peptide identifications is shown in Appendix A and a non-redundant list of all protein identifications and their four varying abundance indicators, the peptide match score summations (PMSS), are shown in Appendix B.
Spectrum sampling methods, like PMSS, as well as peak area based approaches require several reproducible peptide identifications per protein in order to produce accurate abundance ratios, thus no significant statements can be made of the numerous protein identifications near the detection limit. Therefore, we considered protein identifications as potential markers discriminating sufficient from poor CFI when one of the following conditions was observed: 1) protein identifications based on at least two peptides found in both samples of one pool but not in the samples of the other, 2) protein identifications found in both samples of both pools with strongly differing abundance indicators between the samples of the two; 3) qualitative difference represented by differing abundance distribution based on HPLC retention time. Furthermore, all findings from the doubly performed comparison had to be confirmed by the third stand alone comparison using a different RP-HPLC column. Protein identifications which met those conditions and were considered for validation are listed in Table 1 and the underlying peptide identifications are listed in Table 2.
The three times higher abundance indicator of apolipoprotein(a) in the pooled samples of patients with very good myocardial collaterization represents the most solid result of the screen, mainly because it was identified in all three comparative studies and because the abundance indicator ratios were very well reproduced. One apolipoprotein(a) protein is covalently linked to one LDL molecule via ApoB-100 through a single disulfide bond connecting their C-terminal regions. This dimeric macro protein forms lipoprotein(a) (Lp(a)), a lipid transporting particle in plasma. ApoB-100 was also identified in the HPLC fractions 44 and 45 where apo(a) was detected. However, ApoB-100 exists in many isoforms of variable molecular size. Consistently, ApoB-100 eluted as a dispersed peak from HPLC fraction 42 to the end of fractionation therefore confounding the real ratio of Lp(a) derived ApoB-100. Thus, it was not possible to confirm the apo(a) ratio with the ApoB- 100 ratio.
Lp(a) is a very interesting particle for several reasons. First of all its physiological function is unknown but as it independently evolved in primates and hedgehogs it must have a specific function. Secondly, Lp(a) is known as risk factor for cardiovascular disease and has shown atherogenic properties. As atherogenesis and arteriogenesis, which is responsible for collateral growth, are based on similar mechanisms, it is conceivable that apo(a) is involved in the promotion of atherosclerosis and arteriogenesis. The high heterogeneity in protein size (300-800 kDa) in the human population makes apo(a) a very special protein. The heterogeneity is based on a varying number of kringle 4 type 2 (K4 type 2) repeats (2-40) coding region in the apolipoprotein(a) gene. This large variation was caused by neutral evolution in the absence of any selection advantage. It has been shown, that individuals with short apo(a) isoforms have higher concentrations of lipoprotein(a) and a higher risk for CAD or preclinical vascular changes (Berglund et al., 2004). On the other hand, elevated Lp(a) levels have been found in centenarians (Thillet et al., 1998). It has been shown that apo(a), especially the variable K4 type 9 repeats at the N-terminus of the protein, may be physiologically operative in modulating angiogenesis, a process involved in the early formation of collateral vessels (Morishita et al., 2001; Schulter et al., 2001). Furthermore apo(a) competitively inhibits fibrinolysis due to its homology to plasminogen (Hajjar et al., 1989) and contributions to plaque and stenosis formation due to interference with transforming growth factor-b activation (Grainger et al., 1994). It is also worth mentioning that Lp(a) levels are particularly affected by apo(a) synthetic rate, which is subject to strong genetic regulation. Thus Lp(a) plasma levels are affected only to a minor extent by age, sex and environmental factors (Utermann, 1989). However, there is a published study which showed that the serum level of apo(a) is inversely associated with the development of the coronary collateral circulation (Aras et al., 2006), which completely contradicts our findings.
Apolipoprotein(a) Isoform and Concentration Determination in All 80 Samples
Apolipoprotein(a) concentration was determined with a state-of-the-art ELISA assay, using antibodies targeting a non-repetitive section of apo(a), the only proven assay to determine isoform independent concentrations (Marcovina et al., 1995). The apo(a) size was determined with pulsedfield electrophoresis (Marcovina et al., 1996a). The immunoassays confirmed the result of our proteomics screen. The seven patients with sufficient CFI from pool A had significantly higher apo(a) concentrations than the seven patients with very poor CFI values used for pool B (97 and 46 nmol/L). Including all 80 patient samples the result was confirmed too, but was not significant when applying the Mann- Whitney-U-Test. The known negative correlation between apo(a) size and Lp(a) concentration (Rifai et al., 2004) was also detected with our samples (Figure 2).
Figure 2: Apolipoprotein(a) size and concentration in relation to sufficient and insufficient myocardial collaterization (CFI value). The obvious negative correlation between apo(a) size and concentration lead to the formation of three groups of patients (small, medium and large sized apo(a) isoforms) and their testing for differences in apo(a) concentration between patients with sufficient and insufficient CFI values.
We concluded that the strong negative correlation between apo(a) size and apo(a) concentration could overshadow the less strong correlation between apo(a) concentration and the degree of myocardial collaterization, we were testing for as described by Marcovina and colleagues testing Lp(a) concentration differences between black and white Americans (Marcovina et al., 1996b). Based on this assumption, we grouped the 80 patients according to their apo(a) size: patients with less than 18 K4 type 2 repeats, patients with 18 to 29 K4 type 2 repeats and patients with more than 29 K4 type 2 repeats. Within the group with medium sized apo(a) isoforms (52 of 80 patients) a significantly higher apo(a) concentration was found in patients with sufficient CFI levels (33.5 and 13.4 nmol/L, p=0.009, Mann-Whitney-U-Test). Even when combining the groups with medium and large sized isoforms (70 of 80 patients) the correlation between apo(a) concentration and CFI value remained significant (31.3 and 11.9 nmol/ L, p=0.033). Within the patient groups with very short and very long isoforms on the other hand, no significant correlation between apo(a) size and CFI value was observed. The complete dataset is available in Appendix C and the results are summarized in Figure 3. Receiver operating characteristic curves revealed that Lp(a) concentrations are not sensitive or specific enough to predict myocardial collaterization by its own (Figure 4). Our results are based on an opportunistic study with a limited data set. Additionally, there is a considerable variation in Lp(a) levels across individuals for a given apo(a) size. These factors might blur somewhat statistical significance. From other studies it is already known that Lp(a) has some, but still ill defined functional implications in vessel biology as reviewed by (Berglund and Ramakrishnan, 2004). One explanation being that apo(a) is only one factor in a system where many factors are needed in order to tip the balance on one side or the other, e.g. atherogenesis or arteriogenesis.
Figure 3: Box plots of Lp(a) concentration as a function of CFI. Median Lp(a) concentration, the number of patients per group (n) and the possibility of the hypothesis being confirmed by coincidence (p, Mann-Whitney-U-Test) are added in the figure. Hypothesis: Patients with sufficient CFI (>0.25) possess higher Lp(a) concentrations than patients with insufficient CFI values. A: All 80 patients B: 70 patients with medium and big sized apo(a) isoforms (K4 type 2 repeats >17) C: 52 patients with medium sized apo(a) isoforms (30< K4 type 2 repeats >17)
Figure 5: Receiver operating characteristic curves (ROC) of Lp(a) concentration as a function of CFI. ROC curves were calculated with apo(a) having more than 17 K4 type 2 repeats (left panel) and medium size with more than 17 but less than 30 repeats (right panel). The area under the curve were 0.668 and 0.771, respectively. The gray lines represent the lower and upper 95% confidence interval. ROC curves were calculated with a web-based, publicly available tool (Eng J. ROC analysis: web-based calculator for ROC curves. Baltimore: Johns Hopkins University. Available from: http://www.jrocfit.org).
Interestingly our results do not confirm a recently published study on this subject (Aras et al., 2006), in fact they are contradictory. In this study the authors did not distinguish between apo(a) isoforms and the apo(a) concentrations measurements. Especially, the apo(a) concentration determination used was dependant on apo(a) isoform size which is not very accurate in a population containing different isoforms. Furthermore, the degree of collaterization was assessed by a qualitative interpretation by echocardiograms rather than an absolute and quantitative measurement as done in our study.
Results show that the recently developed screening platform (Stalder et al., 2008) is applicable to clinical questions and is capable of delivering preliminary results. With the equipment used, the approach is limited to the ~120 most abundant proteins and thus is not capable to detect lower abundant proteins.
The screen suggests a correlation between the degree of myocardial collaterization, represented by the CFI value, and the concentration of apo(a) in blood plasma. While validating this result by determining apo(a) concentration and apo(a) size in the 80 patient samples, the negative correlation between apo(a) concentration and apo(a) size was confirmed as well as the result from the proteomics screen. We hypothesized that the strong negative correlation between apo(a) size and concentration overshadows the less strong correlation between apo(a) concentration and the degree of myocardial collaterization, thus grouped patients according to their apo(a) size isoform. Within the group of patients with medium sized apo(a) isoforms a significant difference in apo(a) concentration (33.5 and 13.4 nmol/L, p=0.009, Mann-Whitney-U-Test) between patients with sufficient and insufficient myocardial collateralization was detected. The mass spectrometry response for any substance is dependent on the amount of moles ionized. The correlation of apo(a) concentration measured by immunoaffinity means and mass spectrometry as done in our study suggests that mass spectrometric analysis of apo(a) could be a promising alternative for antibody-based apo(a) concentration determination in serum. In combination with spiking of isotopically labeled, kringle specific peptides (AQUA) it would even become possible to determine the isoform of apo(a) (Gerber et al., 2003).


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2724 North Federal Boulevard 5, Denver, Colorado
$474,9002 Beds1+ BathsActive
$474,9002 Beds1+ BathsActive
2724 North Federal Boulevard 5, Denver, Colorado 80211 - Coveted end, rear unit in desirable Jefferson Park! Live the ideal urban lifestyle with incredible city and mountain views in this immaculate 2 bed, 4 bath townhome just minutes from Jefferson Park! Buyer's choice of 3 designer finish packages which boast quartz countertops, unique backsplashes, and custom tile work. 1378 SQFT of meticulously designed living space make up this modern masterpiece. In close proximity to the best of Denver, including LoHi, Sloans Lake, and Larimer Square, this unit, with an oversized 427 sqft rooftop deck, is not to be missed. Contact our Sales Team today for more information or to schedule a tour.
Listing Courtesy of REDT LLC
"Bob Maiocco exceeded our expectations on every level. Not only does Bob have a very solid knowledge of the real estate market, his negotiating skills are second to none. Bob has a calm and even demeanor when dealing with sometimes stressful situations that can arise when buying or selling real estate. We could not have asked for a more strategic partner to help us navigate the process of making our largest financial purchase."


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Tuesday, February 7, 2017
{Book Review} Confrontation with Evil: An In-Depth Review of the 1949 Possession That Inspired the Exorcist by Steven A LaChance
by Steven A LaChance
Publisher: Llewellyn Publications
Release: February 8th 2017
Genre: Non-Fiction-Adult: Paranormal
How I got it: Received a copy from the Publisher via NetGalley
Known as the 1949 St. Louis Exorcism, the story of possessed child Roland Doe was immortalized in the groundbreaking novel and film The Exorcist. Much has been written about the case, but the truth has been shrouded in secrecy...until now.
Join Steven A. LaChance, as he shares the shocking evidence for how a family's grief over the death of an aunt progressed into a full-blown demonic possession. While the conventional story is that Roland Doe brought the demonic infestation upon himself, LaChance convincingly suggests an alternative interpretation, and provides new insights into the nature of possession itself.
The events of 1949 culminated in grueling exorcism rites, but the story doesn't end there as LaChance guides readers through the stunning aftermath that forever changed the Catholic church and the city of St. Louis.
Review: Having read Mr LaChance's other books and knowing some of his history with the paranormal, I have to say I am surprised that Mr LaChance even thinks about things like this case much less researches and writes about them. With that said, I do think that Mr LaChance offered a lot of insight and provide new view points to this case. I was already familiar with the 1949 St Louis Exorcism but this book offered much more in depth background to the case. I enjoyed Mr LaChance's writing style and how he interjected his own opinions based on his experience and beliefs.
This book pushes passed just the exorcism case, and goes beyond telling us about the repercussions that followed this case. Not just in St Louis but in the Church too. I feel Mr LaChance did a wonderful job digging into and researching this case. Over all an interesting look at an old case.
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Charitable organizations need help and often ask for money around Christmas and at the year's end. You may be familiar with the bell ringers of the Salvation Army and their red buckets near store entrances.
The Salvation Army, the Red Cross, police and firefighter organizations, local food pantries, and local homeless shelters are all worthy causes that ask for contributions this time of year.
Will you or your family be giving money to a charity this year?
Now it's your turn: If you were giving money to a charitable organization this week, which one would you choose? Explain why you would choose that particular organization. For instance, do they accomplish something that's important to you? Do they reach a type of person that you care about?
You may be interested in these other Christmas prompts:
Dylan Thomas: His Christmas Memories and Yours
Write Your Own Christmas Carol
That First Christmas: You Are There
Write the Christmas Account from a Different Perspective
Copyright © 2015 by Sharon Watson
Photo: St. Louis Temple Corps and Community Center
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Are your writers struggling? Do you wish you could figure out why your children won't write? Would you love to have a peaceful writing class experience?
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|Check out the innovative The Power in Your Hands: Writing Nonfiction in High School for your complete high school writing curriculum needs. If you have a storyteller at home, try Writing Fiction [in High School] with hundreds of examples from popular fiction and classical literature.
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From Civil to Human Rights
Dialogues on Law and Humanities in the United States and Europe
Helle Porsdam
Chapter 3: Transatlantic dialogues, past and present
Helle Porsdam
It was not until shortly before he died in 1916 that the American writer Henry James became a British citizen. And if it had not been for the refusal of the United States to enter World War One until late in the game, he most probably would have remained an American citizen. James had been a traveller all his life and had written extensively about it. In his travel writing, as in his novels, the theme that critics came to refer to in a rather Euro-centric manner as 'the international theme' – that is a comparison between Europe and the United States – is all-pervasive. 'Europe', as a physical entity but also a concept, had been very important in the Jamesian household, Henry James tells us in his Autobiography. Brought up by 'parents homesick, as I conceived, for the ancient order', whose 'theory of our better living was from an early time that we should renew the quest of the ancient', he was aware from a very early age of the existence of two different worlds, the Old and the New.1 During his childhood and adolescence, the James family travelled extensively in Europe, and going back and forth between Europe and the US became for young Henry a very natural thing to do. Constant exposure to two different sets of values and lifestyles helped create in him an awareness of 'otherness'. James's attitude toward Europe – the ancient – was by no means unambiguous, however. As a concept of the ancient, Europe possessed a...
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Are you a Road Warrior or a Cyberwarrior?
For Indie book writers, only two avenues lead to getting your work into the hands of readers: Road Warrior, or Cyber Warrior. You do not have to take your pick. They combine nicely. But you only have these two choices. It simplifies things. Road Warriors enjoy eye-to-eye contact with their readers. Road Warriors meet their… Continue reading Are you a Road Warrior or a Cyberwarrior?


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jacket & dress: Smart Set
scarf: Market?
leggings: LuLu Lemon
boots: Shoe party
Today I'm thankful for my yoga class. I so look forward to it.
Wonderful colors, nice outfit. And your joyful poses are inspiring.
You always come up with great color combos! Love this outfit!
So great! Would love some red boots.
I LOVE the red boots! I need a pair of those myself ;)


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Bestselling Author | Minister | Empowerment Coach | Social Worker | Certified Life Coach | Psyd Candidate | Clinical Therapist
Andrea Monie Davis CEO and Founder of Life Movement Empowering Ministries, L.L.C. which serves to empower women, men, children, and families. LMEM was developed out of Andrea's desire to serve individuals that desire to do better and progress through life, but seem to remain stagnant. LMEM teaches people to progressively move through life, set and accomplish goals, and live life with an empowered mindset. She will be receiving her Doctorate in Psychology with a specialization in Mental Health Administration in 2016. Her first book now available on amazon 'Deputy for Christ: A Working Journal & Guide for Spiritual Warfare by Andrea M. Davis Link: https://amzn.com/B01IUECUJ6


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Recent medical advancements in oncology care are enhancing outcomes for cancer patients. Optune, a new, leading-edge treatment for glioblastomas (GBM), is helping to pave the way, resulting in minimal side effects and better quality of life for those who receive it.
A group of Cancer Care Northwest (CCNW) physicians, Medical Oncologists Dr. Robert Gersh and Dr. Srivalli Gopaluni, and Radiation Oncologists Dr. Christopher Lee, Dr. Jason Call, Dr. Stephen Thatcher, Dr. Aaron Wagner, Dr. Robert Fairbanks, and Dr. Wayne Lamoreaux, are certified providers. Offering this new cutting-edge technology will enhance the quality of local medical care, and add another component to the cancer-fighting treatment array CCNW provides.
"Optune is a milestone for patients with GBM, a challenging and aggressive form of cancer. CCNW has established a multidisciplinary team approach, closely working with neurosurgeons, radiation oncologists and medical oncologists to bring the latest advancements to the Inland Northwest. Although Optune is not a cure in treatment, it has shown to improve a patient's overall survival by several months," says Dr. Gopaluni.
Optune is a portable device that delivers treatment through ceramic discs within an adhesive bandage called "transducer arrays." These discs are placed directly on the scalp, allowing the arrays to directly target the brain tumor. Optune then creates alternating "wave-like" electrical fields called Tumor Treating Fields (TTF) that travel across the upper part of the brain in different directions to help slow or stop GBM cancer cells from dividing.
"Optune has shown to improve cognitive function, quality of life, and helps patients establish a daily lifestyle outside of their illness. As it is patient operated, patients gain a sense of control over their illness and feel motivated to use it. It is always nice to see the field of oncology advance to a point where the latest technology, and its application, enables patients live a life as close to normal as possible .We are very excited for our patients," says Dr. Gopaluni.
Cancer Care Northwest is currently the only oncology practice in the Inland Northwest to offer both the prescribing and planning of Optune for GBM's.
For more information on Optune, please visit www.optune.com.


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MGM China Holdings says the Jan. 29 opening of its MGM Cotai resort will kick off a series of celebrations designed to showcase the HK$27 billion property.
Grant Bowie is the chief executive officer and an executive director of MGM China Holdings Limited. He has been the president of MGM Grand Paradise since August 1, 2008.
With over two decades of experience working in the hospitality industry, Mr. Bowie joined our Company after approximately four years as the president and general manager of Wynn Macau. Prior to that, he contributed to the growth and development of Park Place Entertainment Corporation's Jupiter operations in Australia from 1987 to 2003. In his 16 years with Park Place Entertainment, he held senior positions in casino, general finance and hotel operations before being appointed General Manager of both Park Place Entertainment's properties in Australia. Mr. Bowie is also an Adjunct Professor in Tourism and Leisure Management at the University of Queensland. Mr. Bowie holds a Bachelor's degree in commerce from the University of Otago in New Zealand.


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This is a CyanogenMod 9 build for the U8150.
Initial release
Turn off animations in developer settings for improved performance.
FLASH THIS RECOVERY BEORE FLASHING THE ROM - Thanks to alanorth http://minus.com/dbl0vdTlBJcEk4.img
GAPPS - market etc - thanks to TeamICS - download
GO Keyboard (temporary measure while I get keyboard the correct size) - download


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O. Henry Biography
1916 · Garden City, NY
by SMITH, C. Alphonso
Garden City, NY: Doubleday, 1916. Very Good. Later. Very good Hardcover, later printing, no dustwrapper. Book has light wear of spine ends, cover edges and corners, light fading covers, cracking hinges. Clean text. Gold gilt top foredge. Please Note: This book has been transferred to Between the Covers from another database and might not be described to our usual standards. Please inquire for more detailed condition information. (Inventory #: 170981)


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The Glitter Guide | Fashion Feature with Krystin Lee
Wednesday, October 2, 2013
8 comments:
I am loving these pictures! What a dream to be featured on The Glitter Guide and be photographed by you!ReplyDelete
Five Minute Style
Krystin is beautiful and you took amazing photos! I can't wait to work with you!ReplyDelete
Anna you are such a talent! MUST work with you one day soon. What a gorgeous feature for both you and Krystin! xoReplyDelete
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Such beautiful pictures!ReplyDelete
Amazing photos Anna! You are so talented.ReplyDelete
Something like come within reach of fashion style sense transform to fine design sense.ReplyDelete
Adam Lambert leather Jacket
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Wiescher Design Standard LicenseBy buying this font you are agreeing to be bound by the terms of this Agreement. This Agreement, in conjunction with the receipt that accompanies each purchase from Wiescher-Design, constitutes the complete agreement between you and Wiescher-Design.1. Allowed usesYou may use the licensed fonts to create images on any surface such as computer screens, paper, web pages, photographs, movie credits, printed material, T-shirts, and other surfaces where the image is a fixed size.You may use the licensed fonts to create EPS files or other scalable drawings provided that such files are only used by the household or company licensing the font.2. Number of usersThe maximum number of simultaneous users is specified in the applicable receipt. All users must belong to the same company or household purchasing the font.3. Third partiesYou may provide the font to a graphic designer, printer or other service bureau that is working on your behalf only if they agree to use the font exclusively for your work, agree to the terms of this license, and retain no copies of the font on completion of the work.You may not provide the font or make it accessible to any other third parties.4. EmbeddingYou may embed the licensed fonts into any document you send to third parties provided such embedding is restricted to viewing and printing that document.You may not embed the licensed fonts into software or hardware products in which the fonts will be used by the purchasers of such products. Such use requires a different license (OEM). Please contact firstname.lastname@example.com for information on OEM licenses.5. ModificationsYou may import characters from the font as graphical objects into a drawing program and modify such graphical objects.You may not modify, adapt, translate, reverse engineer, decompile, disassemble, or create derivative works based on the licensed font itself without Wiescher-Design' prior written consent.6. CopyrightThe font and the accompanying materials are copyrighted and contain proprietary information and trade secrets belonging to the foundry owning the font. Unauthorized copying of the Product even if modified, merged, or included with other software, or of the written materials, is expressly forbidden. You may be held legally responsible for any infringement of the foundrys intellectual property rights that is caused or encouraged by your failure to abide by the terms of this Agreement.7. TerminationThis Agreement is effective until terminated. This Agreement will terminate automatically without notice from Wiescher-Design if you fail to comply with any provision contained herein. Upon termination, you must destroy the written materials, the Product, and all copies of them, in part and in whole, including modified copies, if any.8. Product UpgradesWiescher-Design may, from time to time, update the Product. Product upgrade pricing may apply.9. Disclaimer and Limited WarrantyWiescher-Design warrants the Product to be free from defects in materials and workmanship under normal use for a period of twenty one (21) days from the date of delivery as shown on your receipt. Wiescher-Design' entire liability and your exclusive remedy as to a defective product shall be, at Wiescher-Design' option, either return of purchase price or replacement of any such product that is returned to Wiescher-Design with a copy of the invoice. Wiescher-Design shall have no responsibility to replace the product or refund the purchase price if failure results from accident, abuse or misapplication, or if any product is lost or damaged due to theft, fire, or negligence. Any replacement product will be warranted for twenty one (21) days. This warranty gives you specific legal rights. You may have other rights, which vary from state to state.EXCEPT AS EXPRESSLY PROVIDED ABOVE, THE PRODUCT, IS PROVIDED AS IS. WIESCHER-DESIGN DOES NOT MAKE ANY WARRANTY OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE.The entire risk as to the quality and performance of the Product rests upon you. Wiescher-Design does not warrant that the functions contained in the Product will meet your requirements or that the operation of the software will be uninterrupted or error free.WIESCHER-DESIGN SHALL NOT BE LIABLE FOR ANY DIRECT, INDIRECT, CONSEQUENTIAL, OR INCIDENTAL DAMAGES (INCLUDING DAMAGES FROM LOSS OF BUSINESS PROFITS, BUSINESS INTERRUPTION, LOSS OF BUSINESS INFORMATION, AND THE LIKE) ARISING OUT OF THE USE OF OR INABILITY TO USE THE PRODUCT EVEN IF WIESCHER-DESIGN HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES.Because some states do not allow the exclusion or limitation of liability for consequential or incidental damages, the above limitation may not apply to you.10. Governing LawThis agreement is governed by the laws of Germany.
Nasalization W00 3D
Ray LarabieAuthor
Ray LarabieTrademark
Nasalization is a trademark of Typodermic FontsCopyright
Copyright (c) 1999 Typodermic Fonts. This font is not freely distributable. Visit typodermic.com for more info.Description
Please read the current Typodermic Fonts license agreement at http://www.typodermic.com/license.html
Tumbletype No2 W01 Regular
Greater Albion TypefoundersAuthor
Greater Albion TypefoundersTrademark
Tumbletype No2 is a trademark of Greater Albion Typefounders.Copyright
Copyright (c) 2008 by Greater Albion Typefounders. All rights reserved.Description
Copyright (c) 2008 by Greater Albion Typefounders. All rights reservedLicense
License in accompanying file
DJB I Love Me Some Brook V2
DJB I Love Me Some BrookStyle
1.00 October 5, 2012, initial releaseCopyright
Typeface (your company). 2012. All Rights ReservedDescription
This font was created using FontCreator 5.6 from High-Logic.com
Grota Sans W00 ExtraBold
Eli Hernandez & Daniel HernandezTrademark
Grota Sans ExtraBold is a trademark of Eli Hernandez & Daniel Hernandez.Copyright
Copyright (c) 2014 by Eli Hernandez & Daniel Hernandez. All rights reserved.Description
Copyright (c) 2014 by Eli Hernandez & daniel Hernandez. All rights reserved.


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0 ThanksSay
Thanks! Thanked!Front Of 46 Rose Avenue Great Neck, New YorkStreet light has been out for several days. Reported the problem twice before in the past to 311. Problem has not been resolved. Light comes on and than goes out for several days. It gets very dark at night and it gets very difficult to see. Accident waiting to happen.· Reported by Afridi · Flag Flagged
311 call center (Registered User)
The Town of North Hempstead has reviewed your complaint. Service Request number 5747 was submitted to the Town's Street Light Division for repair.
Please feel free to call the Town's 311 Call Center for future assistance by dialing 311 if within the Town or (516) 869-6311 if outside the Town. North Hempstead's 311 call center is open from 7:30 am to 7:30 pm Monday to Friday, Saturday from 10:00am to 6:00pm and Sunday from 10:00am-5:00pm.
Closed Afridi (Registered User)Light has been repaired. Thank you


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Royal Bank Of Scotland To Pay £29M Price-Fixing Fine
The U.K. Office of Fair Trading said Tuesday that the fine was reduced from £33.6 million ($50.6 million) because RBS admitted to the conduct, which occurred between October 2007 and February or March 2008, and agreed to cooperate with the investigation.
"Any company that discloses confidential future pricing information to its competitors risks a substantial...
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Are you looking for an apartment in Las Vegas? Next month, one of the units in our fourplex property will be available for rent. The unit is located in the second floor with two bedrooms and two baths. Water, garbage and sewer fees are free or waived. The requirements? Well, we believe that ... [ Read More ]
Archives for August 2012
Aunt Leony's Visit
Last week, my hubby's aunt who lives in Indianapolis visited us and stayed with us for a week. Despite my bulging tummy, I'm so glad that I was still able to keep up with the whirlwind of activities during Aunt Leony's vacation here in Las Vegas. We visited the Las Vegas Strip a couple of times ... [ Read More ]
Still Preggy!
Thanks to all my FB and blogger friends for checking on me for not being able to post for more than a week now. I'm still very much preggy and Georyl's little sister still wants to stay a little bit longer in the comfort of her mom's womb (that's me, of course!). What's keeping me busy? Well, ... [ Read More ]
Belated Retirement Greetings
I can't believe that it's been more than a year since my husband retired from the service. I've always wanted to give him something special as a way to celebrate his retirement but I wanted to buy something from my own income - and for a stay at home wife and mother, it's not that easy. Last ... [ Read More ]
Have You Ever Worn Contacts?
Have you ever worn or considered wearing contact lenses? Some people wore contact lenses for fashion purposes (changing their eye color) but I had to use them before because of my poor vision. It wasn't continuous though and the periods when I wore them mostly depended on my schedule or routine, ... [ Read More ]
Real Estate Shopping
Ever since we I came across Robert Kiyosaki's books and his principles regarding real estate investment, I've been interested in the status of the real estate market. I knew when the real estate market was down since there were lots of properties being sold at really low prices but I've noticed that ... [ Read More ]
Famous Footwear Back to School Promo
This post brought to you by Famous Footwear. All opinions are 100% mine. It's almost time for school opening! Are you ready? For children, back-to-school also means new shoes, school supplies, bags and other school stuff. Yes, it's time to go shopping, Momma! If your pupil or student needs new ... [ Read More ]


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The toughest two-week stretch on the schedule is in the rearview mirror, and Ohio State remains unbeaten.
A rocky nonconference slate made the opening month of the season something of an adventure for Michigan, but the Wolverines survived without a blemish on their record as well.
Both programs point toward arguably the most heated rivalry in the country as the most important matchup all season regardless of rankings or what's at stake in the Big Ten or nationally, and that won't change in late November. But so far, everything appears to be lining up for what could be an epic confrontation with far-reaching implications aside from just bragging rights.
And if the Buckeyes and Wolverines stay on track heading into the second half of the season, there might even be an encore to The Game one week later in Indianapolis in the Big Ten championship game.
Game: Ohio State-Michigan
What's at stake: Michigan hasn't done much to put itself in the national title conversation just yet with some unimpressive victories over Akron and Connecticut, but if the Wolverines keep that record unblemished, they ultimately would have to be in the discussion. Ohio State is already a prominent part of the championship picture now that it has knocked off Wisconsin and Northwestern to push its winning streak to 18 games overall, and the Buckeyes have long circled the trip to Ann Arbor, Mich., as one of the biggest hurdles in "The Chase" for a trophy.
But just in terms of the feud between the rivals, Urban Meyer got a leg up on Brady Hoke by winning the first head-to-head matchup between the coaches last year at the Horseshoe. And in the battle for public perception and in the recruiting wars, no victory means more than one against "Ohio" or "That Team Up North."
Roadblocks/derailment opportunities: The Wolverines have made life difficult for themselves with costly turnovers, and they spent their first bye week making sure they weren't tripping over their own feet heading into conference play. Quarterback Devin Gardner showed marked improvement as Michigan opened up Big Ten action with an easy victory over Minnesota, operating the offense with the type of efficiency that was expected during training camp and appearing to right the offensive ship. The Wolverines will have to keep playing with that sharpness during a brutal three-game stretch that starts after its second second bye week, visiting Michigan State on Nov. 2, hosting Nebraska and then going back on the road to take on Northwestern.
Ohio State, though, is mostly playing against itself and Meyer's high standards until it faces Michigan. With a favorable Big Ten draw that didn't include Nebraska or Michigan State this season, the Buckeyes will be heavy favorites in each of their next five games, particularly now that the offense is starting to look more like Meyer envisions thanks to the return of Braxton Miller from injury and Carlos Hyde from suspension. A visit from Indiana's high-powered offense Nov. 23, a week before The Game, might be the toughest remaining test.
How it unfolds: With the spread offense clicking, the front seven developing ahead of schedule and a couple of bye weeks to potentially tweak the retooled secondary following Christian Bryant's injury, everything appears to be in place for Ohio State to hold up its end of the deal to set up a memorable meeting in Michigan. The Wolverines figure to have the tougher time staying unbeaten into late November, and they've also shown more vulnerability, thanks to those narrow wins outside of conference play.
The focus will remain on Gardner and his ability to cut down on mistakes as the race in the Legends Division heats up, though the return of linebacker Jake Ryan could provide a lift on the other side of the ball and maybe offer a bit more breathing room for the offense if he regains his all-conference form after tearing his ACL in the spring. But if the turnover issue pops up again, particularly on the road, knocking their rivals out of the national title race might end up being the biggest motivator for the Wolverines.


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Quality Products + Quality Service = Quality Results
When you've been around as long as we have, you see companies come and go. That's why we've selected the highest quality and most reliable brands to offer our customers. We're proud to recommend companies recognized for innovative technology and backed up by longstanding reputations for quality and dependability. Plus, you can save BIG on qualifying high-efficiency equipment through NJ's Home Performance with Energy Star Program. Now you can afford to get the best for less!
Here are some of the great products we offer. If you don't see what you are looking for, just send an email or give us a call.
|Mitsubishi Electric Cooling & Heating
|Bradford White
|American Standard
|and more...
Trane – Trane is a leading
global provider of indoor comfort systems and comprehensive facility solutions. Its
offerings include energy efficient heating, ventilating and air conditioning systems,
service and parts support and advanced building controls. Each Trane system is designed to
meet the specific needs of customers who want heating, cooling, dehumidifying and air
cleaning systems for residential, commercial, institutional and industrial
- Carrier – continues to lead through innovation, especially with their new energy-saving Hybrid Heat system and their Greenspeed Intelligence. Redding's consistently meets Carrier's high standards of technical expertise, business practices and customer service, which include NATE certification, the industry's recognized certification for service technicians.
- Payne – is a nationwide brand that provides home comfort equipment that offers homeowners consistent, affordable and hassle-free indoor comfort. Their solid lineup of heating and air conditioning equipment provides great products at the right price.
- Burnham by U.S. Boiler Company - is a leading manufacturer of boilers, furnaces, radiators, air conditioning systems, and related accessories for residential, commercial, and industrial applications. The Burnham group provides high value, high quality products backed by superior service.
- Weil-McLain - offers a full range of comfort heating products to meet the unique needs of your home and lifestyle, including gas-fired boilers for the best combination of gas heating and energy efficiency and oil-fired boilers for maximum efficiency and reliability in oil heating products.
Electric Cooling & Heating - Mitsubishi Electric Cooling & Heating
(Mitsubishi Electric) is America's #1 selling brand of ductless (mini-split) cooling and
heating technology and leads the market of intelligent air-conditioning and heating
technology, including ductless air conditioners and heat pumps.
Mitsubishi Diamond Contractor
Redding's is proud to be a Mitsubishi Diamond Contractor in Greater Princeton and surrounding Mercer County, New Jersey areas. This means we're an independent dealer that has reached the highest level of accreditation from Mitsubishi Electric Cooling & Heating. Factory trained in their ductless air conditioners and heat pumps products, Redding's has invested the time to develop personalized, hands-on expertise in sales, installation and service at one of Mitsubishi Electric's approved training centers.
- Unico - provides homeowners, business owners, and commercial operations with high performance and superior comfort no matter when your home or building was built or what its physical configuration. With Unico installing heating and air conditioning is always a possibility - without compromising design or architectural integrity.
- Rinnai - offers a tankless natural or propane gas-fired water heater that replaces traditional hot water storage tanks.
- Rheem - has been a trusted brand since 1930, offering high quality water heating products and reliable customer service for your lifestyle and peace of mind.
- Bradford White - offers an extensive line of residential, commercial and industrial water heaters and also manufactures combination space heating equipment based on a tank-type design. Bradford White's expertise in water heating includes gas, electric, oil, solar and indirectly powered models.
- Honeywell - provides a variety of air purification products. Ultraviolet (UV) Air Treatment Systems zaps surface mold and airborne bacteria passing by the UV lamp with proven technology used in hospitals and water treatment for several years. Honeywell's Whole-House Electronic Air Cleaner offers a superior level of filtration by using advanced, 3-stage filtration technology to trap and filter airborne particles like dust, cat dander and smoke.
- Aprilaire - provides a variety of air purification products. Ultraviolet (UV) Air Treatment Systems zaps surface mold and airborne bacteria passing by the UV lamp with proven technology used in hospitals and water treatment for several years. Honeywell's Whole-House Electronic Air Cleaner offers a superior level of filtration by using advanced, 3-stage filtration technology to trap and filter airborne particles like dust, cat dander and smoke.
Redding's also supplies a range of quality plumbing products, including world leaders like:
American Standard -
Bathroom & kitchen products
- Moen - Bathroom & kitchen products
- Kohler - Bathroom & kitchen products
- Grohe - Bathroom & kitchen products
- Toto - Bathroom products
- Aqua Pure - Water purification products
Call us today to ask about our products. Selecting products that match your home and your needs will provide superior comfort and increased energy efficiency for years to come. We'll show you how.


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We offer a turn key, hands off solution for your business's Digital Signage needs
Software SaaS
MCA will design a complete, customized and web based learning management system which your employees can access and learn on their own time. Our system is designed using WordPress and the popular Sensei LMS plugin. All features that you need for your situation are included so that the system runs as you need with the modules in place and ready to use.
You'll work with MCA's script development experts in putting together concise and effective scripts to be used in each module of your program. We have developed a system that allows you to relay your expertise on the lesson subject, and our script writers to transfer that over to an effective presentation that your employees will get maximum results from.
Whether it is in the field shooting procedures and techniques or in our studio shooting a demonstration, we have the ability and video production experience to put together compelling training videos. We've worked with customers ranging from a regional carrier truck line all the way to a Fortune 500 Grocery Chain, in producing video training presentations that have the ability to produce results. View Examples
Our photography department uses high end camera equipment and years of photography experience to take the exact picture needed to accentuate specific procedures and techniques mentioned in a training lesson. All photos are expertly edited using high end industry software to give maximum resolution to enhance the viewing experience. View Examples
MCA will train and work with the people you designate as administrators for your program, so that they can add and delete employees, follow progress of individuals and change out specific items like quiz questions and downloadable documents. We also offer a monthly management program for a small monthly fee that will provide hands free updates and management.
Nothing works in a good training program without good analytics and data. And with our programs, we include easy to understand and access information that helps you understand both how well the system is performing in providing the right training to the right audience. We set up the reporting part of the system to give a customized set of results that you need to go forward.
With a Digital Signage System from MCA, Content is King!
With Dyna-View, content is king. It's what makes your message stand out from the crowd, compels your customers to take action, and what drives a good digital signage system. It's why we include content with every player out of the box!
And when it comes to content, not just some PowerPoint templates that just puts something on your screen. We're talking beautiful, fully customized, high definition content that puts your message out there in an engaging, pleasing manner. All expertly edited and put together by a video production team with over 40 years of combined video editing experience!
Your logo, your colors, your content (be it video, images, graphics and information), along with things like live weather feeds, news, sports, stocks and business info and 4K product video.
We're so committed to provide the best Digital Signage Experience for our clients, we developed our own brand
Our experience with out of the box signage players and software is that for the most part they would not perform to the standards we wanted to run on our boards. So we developed our own brand, Dyna-View.


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|Reviews for Not With a Bang but a Whimper
| Corianna15 chapter 3 . 12/26/2014
loving this story...not loving that it ends there...after two years I'm not optomistic about an update. However if you're still out there...please?
| WhisperingFantasies chapter 3 . 3/31/2013
Oh my gosh, Moriarty is evil and I could feel Sherlock's pain as he watched his best friend, the man he loves (platonically, of course) being tortured! I cannot WAIT for the next chapter!
| Servant05 chapter 3 . 1/11/2012
Please, oh please, OH PLEASE come out with another chapter! I have to know what's going to happen!
This is such a good premise and is written fantastically! I have recently became a fan of this particular fandom and love hurt!John. You are actually the first that I have reviewed after spending countless hours reading multiple stories.
Please *big puppy eye* Please, let me know what happens soon?
Thanks for the great read!
| Noremac1 chapter 3 . 11/29/2011
This looks pretty good. More!
| charliebrown1234 chapter 3 . 11/11/2011
I was not expecting this from the title of the story. Because of that, I like this story even more. You have Moriarty acceptably crazy, Sherlock perfectly brilliant and John as John the BAMF. Wonderful, fantastic stuff. Please update soon. :)
| Jodi2011 chapter 3 . 11/3/2011
Poor John suffers while Sherlock plays Moriarty's game. What if Sherlock said no and refused to play? Could John possibly be any worse off?
I really don't like Moriarty, especially when he hurts John. I hope John still has the strength and the will to escape after this.
Update soon please!
| Skyfullofstars chapter 2 . 10/29/2011
Okay, I am definitely waiting for the next chapter to be up.
Please post more soon.
And thanks for posting!
| Electryone chapter 2 . 10/25/2011
This is really interesting so far. I'm dreading seeing what Moriarty does to John. That being said, I hope for an update soon!
| dianaj2w chapter 2 . 10/12/2011
OMFG... great story... poor John, ... please conti soon
| Xenitha chapter 2 . 10/12/2011
Okay, now where is the next chapter? Please?
| xjessicafaithx chapter 2 . 10/12/2011
This fic has just begun...and oh the amount of love I already have for it!
Pins and needles here.
| constantlycold chapter 2 . 10/12/2011
Oh! I'm loving it so far! Keep up the great work! I don't mind a little darkness... It's Moriaty!
Good job!


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Title: In the Flesh by Portia DaCosta
The Ladies Sewing Circle Book 5
Publisher: HQN Books
Genre: Historical, Erotic, Romance
Length: 382 pages
Book Rating: C+
Review Copy Obtained from Publisher Through NetGalley
Society already believes she's a scarlet woman. Why not become one?
Posing nude to appease her now ex-fiancé perhaps wasn't the most prudent idea Beatrice Weatherly has ever had. With the photographs scrutinized up and down the ton and her brother running them into debt, Beatrice's hopes of making a respectable marriage are dashed.
After one glance at Beatrice's infamous racy cabinet cards, wealthy, powerful Edward Ellsworth Richie is soon obsessed with Beatrice's voluptuous figure. His indecent proposal—one month of hedonistic servitude in exchange for enough money to pay her brother's debts—is one she can hardly refuse.
Determined not to let the rogue best her, Beatrice sets out for the infamous House of Madame Chamfleur to learn how to appease Edward's well-known appetite. Soon the couple is playing out exquisite fantasies…and feeling emotion that goes deeper than flesh. But Edward harbors a shocking secret, and Beatrice must decide if she's prepared to give up everything for a man who can offer her nothing, but who means everything.
The Review:
Portia Da Costa's newest historical romance In the Flesh is an enjoyable romance that is exquisitely sensual and highly erotic. Having obtained copies of her risqué cabinet cards, Edward Ritchie is immediately intrigued by Beatrice Weatherly and quickly arranges a meeting with the infamous beauty.
In the Flesh is a fast paced and sexy romp. The characters are immensely appealing and delightfully well-developed. Beatrice is extremely likable and rather forward thinking for the time period. No nonsense and pragmatic, Beatrice agrees to Ritchie's terms and unashamedly embraces her sexuality.
Ritchie is a complex character with a complicated past. He attempts to keep his relationship with Beatrice strictly physical with few emotional ties. Much to Beatrice's dismay, he does not rush their sexual relationship. Instead he gently tutors and encourages her to explore her sexuality with and without him.
A secondary subplot involving Beatrice's brother and maid and Ritchie's servant provide added interest to In the Flesh. This storyline is fairly low key yet very tantalizing. Their scenes were quite entertaining and very, very sexy.
While the overall story was quite enjoyable, the romance between Beatrice and Ritchie felt rushed. Their time together was limited and mostly spent in the bedroom. The conflict between Beatrice and her ex-fiancé was predictable and added little to the plot. The storyline involving Ritchie's past is also quickly resolved and it is wrapped up a little too neatly.
In the Flesh by Portia Da Costa is a fast paced novel with engaging characters. The sex scenes are plentiful and quite titillating. If you are a fan of historical erotic romance then you are sure to enjoy this deliciously sensual story.


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To adapt to changing times, the JTB Group is expanding beyond its present tourism and travel business, and developing "cultural exchange" as a core business for creating and promoting opportunities for people to come together and interact.
In particular, we have recently been rolling out initiatives in the food and agriculture tourism business, given the current worldwide interest in Japanese cuisine and Japan-made products.
As part of these efforts, we are working to publicize the specialty products of different regions of Japan, and have held a series of overseas exhibitions (focused on the Asia region) since 2014 under the name: Japanese Foods "Premium" Trade Fair.
This year, the 4th Japanese Foods "Premium" Trade Fair will be held at five regions, Taiwan, Hong Kong, and Singapore as well as Malaysia and Japan for the first time.
The Japanese Foods "Premium" Trade Fair in Malaysia looks to help buyers from Malaysia recognize and discover products from Japanese suppliers, with the aim of building new distribution channels for exports from Japan.
Today, Japanese products are popular in the various regions of Asia, and are becoming firmly established. However, there are still many products that have yet to gain the recognition that they deserve.
We hope you will participate in this fair, and take the opportunity to explore the new possibilities offered by agriculture, forestry and fisheries products from Japan.
Please look over the details provided in this document (Participation Invitation and Instructions), and then apply.


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I consider it a win that I did not eat Halloween candy for breakfast today. Unfortunately, I'm sure my continual snacking on it throughout the day is just as worse. Those mini kit-kats are just so addicting!! Tomorrow I will try to be better... You see, I suffer from Severe Sweet Tooth and Zero Will Power Syndrome. Sufferers from SSTZWPS always want something sweet and whenever faced with sweets, have a hard time saying no. We can never eat just one cookie, we'll go back and nibble until they … [Read more...]
M&M Cookies
I love dessert but I don't usually eat candy. I'll get Junior Mints at the movies and occasionally I'll crave a Snicker's or a Reese's Fastbreak or just a plain Reese's. But if I had to choose one candy to snack on, I'd choose M&Ms. So when I as at Hobby Lobby the other day on my lunch break, a bad habit that is affecting my paycheck, I saw these harvest colored M&Ms and couldn't resist buying them. They also happen to sort of be USC colors so they're perfect for game … [Read more...]
4th Of July Popcorn Party Mix
"We hold these truths to be self-evident, that all men are created equal, that they are endowed by their Creator with certain unalienable Rights, that among these are Life, Liberty, and the pursuit of Happiness." -The Declaration of Independence I love the 4th of July. Not because of the barbeque, or the fireworks, or because it's an excuse to obnoxiously sing country songs (I do that year round...). No, I love the 4th of July because I love America and I love history and I have a hefty … [Read more...]
Easter Blondies
I dropped the ball on posting recipes in advance for Christmas. And Valentine's Day. And even St. Patrick's Day. But I will not be behind for Easter. Thus, I bring you Easter Blondies. A good few weeks before Easter. You're welcome :) … [Read more...]
Valentine's Day Puppy Chow
Well Stephanie, I guess I take the cake on baking procrastination... In my defense, I made this specifically for Valentine's Day at the office but just haven't blogged about it until now. The beauty of puppy chow, however, is that it is not holiday specific. You can make it for Easter or 4th of July or tomorrow, just because you feel like it! … [Read more...]


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Indeed, the Red Devils' home form in Europe is something of a worry considering they slipped up at home to Benfica and Basel in the Champions League and Sir Alex Ferguson will want his team to steamroller the opposition so he can give several first-teamers the night off for the trip to Spain next week.
United are a best price 8/15 (Coral free bets) to claim victory and their performance at White Hart Lane on Sunday was pretty impressive, with the visitors running out 3-1 winners including two goals for Ashley Young.
The winger is hitting goalscoring form as the season approaches the business stage and can be backed at 9/1 (Ladbrokes bookmaker) to break the deadlock, while Wayne Rooney is the 4/1 favourite with the same firm although the England forward might be given a breather ahead of Sunday's Premier League clash with West Brom.
Athletic Bilbao recovered from a 2-1 defeat away to Lokomotiv Moscow to beat the Russian side 1-0 and go through on away goals, while their recent domestic form has been suitably impressive.
Home wins over Malaga and Sociedad have sandwiched a 2-2 draw at Villarreal and the Spanish side are a 6/1 poke (bet365 £200 free bets) to claim an away win which could put them on the verge of a big shock.
Fernando Llorente is the class act for Athletic and he can be backed at 17/2 (Unibet) to score the first goal of the game.


text: A swung rhythm is a rhythm where each beat is divided into two parts, and the first part becomes longer and more accented than the second part. Performers of swing play notes that are equally notated with unequal durations, and repeat the unequal patterns continuously. Swing is also used to describe a sense of rhythmic feel often found in Jazz music. Swing is also a genre of music in itself.