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Review by Jacen Solo
Reviewed: 01/19/03 | Updated: 01/19/03
If you hear the name of the game,run and never look back
IF there's a genre that can be said that appeals to almost everybody,shooters are probably it.I mean,nearly everybody loves blasting stuff into oblivion right?And with the power of the current consoles,it's looking more and more beautiful.GunValkyrie is another entry in the genre that tries to draw you in with the premise of adrenaline pumping action.But does it truly deliver?
Gameplay:Since the advent of the 3D action/adventure genre by Tomb Raider,most(if not all) shooters nowadays take the route.There's nothing wrong with it actually,it's been proven to be successful for lots of games.And while GunValkyrie certainly is not the best title in the genre,it still is one of the most notable(although not as you'd might expect) for quite a while.While it certainly lives up to the premise of blowing stuff up,there is an undeniable monotony to it.Objectives are nothing more than kill everything in a single area,get to said location,elimate so and so or sometimes even all three all rolled into one.Ok,killing everything and enjoying mindless carnage IS fun.But after the fourth or fifth mission with the same objective,it starts to lose its fun factor and reverts to just plain boring.
By the fourth or fifth mission,you'll probably be yawning at the boring storyline and the lack of continuity that should accompany the gameplay.It's just mindless shooting.Sure,there's the pretense of having a storyline with Poe's report(some pages of backstory you get to read in the mission briefing)but it's not enough.There're barely any cutscenes in the game and most of those are grouped together near the end.And speaking of end,GunValkyrie is rather short.Just when you finally managed to get good at it,it ends.Sure,I understand everything has to end but only having 10 levels is just too short.
And to make matters worse,there're only 3 main types of guns with several different variations.You might think this is a case of quality vs quantity.Absolutely…WRONG!The guns suck for the most part and the variations are just subtle changes to the way damage is dealt.Sega COULD come up with creative weapons,take a look at Virtual On for some great weapon designs.In this case though,it's just laziness at work.There were supposed to be 8 different weapons to be included in the game when it was first announced…
There's not much character variety either.You only get two to choose from,Saburouta and the requisite ample bosomed female,Kelly.Saburouta's your basic strong but slow hero while Kelly plays the quick but weak heroine.Sure,they have different guns and attacks but it doesn't explain the lack of original characters.It's like the developers wanted to add in something good but time didn't permit it.Just take a lot at the trailers of the game and you'll see what I mean.The trailers had the characters doing fantastic stuff,running and dodging while firing furiously.The thing is,nearly everything is missing here.The promised 'revolutionary' control scheme is bull and the smooth combat moves are too hard to pull off.
Another thing that's weak is the A.I.To put it bluntly,it's dumb.The enemy will constantly get stuck behind walls,move around in circles or even ignore you as you blast them.Unless these guys actually like pain like the Yuuzhan Vong(give yourself a cookie if you know who they are),I think it's safe to say the artificial intelligence needs workIts especially noticeable in the early levels since they're littered with debris.The enemies will constantly get hung up on pieces of debris or even behind rock columns.
Gameplay rating:5/10
Graphics:For a first generation title,GunValkyrie looks good.Nicely detailed character models,great textures(although they tend to repeat quite a bit) and spectacular particle and lighting effects abound in the game.Everything moves at an even frame rate too.That's quite a feat since there's definitely no lack of action in this game.Some effects,like the bits and pieces that fly off an enemy as you fire at it is really satisfying to watch.There's no feeling better than moving down rows upon rows of bugs and watching them break apart as the hail of lead blows through their exoskeletons.It's such a rush,I tell you…
There're a couple of strange things though.While the outdoor levels,don't have any fog,the indoor levels sometimes do.It's especially noticeable if you drop down a deep hole in the indoor stages.If you look down while you're falling,you can see the fog just below your feet working quickly to render the floor you're dropping into.I'm no expert in programming but it looks to me that bad code is at work here.What's the harm in doing more polishing?Also,sometimes(VERY rarely),the collision detection goes out the window.I've once had four bugs go through a wall and staying there.Needless to say,I had to restart the mission because I had to kill them to complete it.It doesn't happen regularly enough that it becomes a fatal flaw but still it is a pain when it does.
Controls:Ok,before you guys(or girls)read any further,you best better prepare yourself.It's going to get ugly,really ugly.Done?I'll begin…
GunValkyrie is the WORST(I repeat,WORST!!!) controlling game I've played.Its controls are awkward and only makes an already hard game neigh impossible.There's NO way to change your controls(what game DOESN'T have a config option?!)so you'd better bear the pain if you want to play the game.The main culprit is the way boosting is handled.You need to press down on the right analog stick AND move it in the direction.Sure,it may sound simple to do on paper but its anything BUT simple.Try doing that when dodging multiple projectiles,fighting the god-awful camera AND jumping from platform to platform…at the same time.Only those with Jedi fingers or something can do stuff like that.Sure,you may get the hang of it in a while,but unless Smilebit only intended to appeal to people with lots of time and patience,it's safe to say most of us will be disgusted at the unfriendly controls.
And the sad thing is without the control problems,GunValkyrie would actually be GOOD.The controls are very responsive and precision jumping is not a problem.Its doing precision jumping and boosting that needs work.If only they had assigned the boosting duties to the unused "A" button(hold A and a direction to boost is how I think it should be done),everything would be fine.Wait…scratch that.ALMOST everything would be fine.What's the problem,you ask?It's the aiming.The cursor automatically autocenters itself after every shot.It's a pain in the unmentionables to keep realigning the reticule repeatedly at the same spot over and over.It's not so bad if you're playing as Kelly since her gun can lock on to multiple targets automatically but Saburouta is almost unplayable at long range since he relies on single shots(he can't lock on)to get the job done.Also,you can't circle strafe.Yup,for some strange reason,you cannot sidestep in a circle.I have no idea why that is since the control's are based on FPS games(left analog stick moves your character while right aims and moves the camera view).A game of this nature NEEDS circle strafing!Without circle strafing,the only ways to get out of harm's way is by repeatedly dashing and shooting in the air(a pain) or run backwards while shooting(also a pain) .
Control rating:1/10
Sound:I can say that GunValkyrie's rather good in this area.The sounds of the guns firing are great.Listen to the Drive Gun(picture an oversized sniper rifle but with a firing rate of a machine gun)mowing down the legions of advancing bugs and you'll definitely know what I mean.The 'blam blam blam' sound it makes plus the crazy vibration the controller makes as you fire it almost makes you believe you're really holding it in your hands.The music is good in some stages but in others it's just average.Naglfar's pit has some of the most haunting music I've heard.It's really nice if you're into that kind of thing(which I am).But if you like your stuff with a more upbeat…beat to it,there're levels with rock music sans lyrics of course.
The enemies' sound good…for the most part.The main enemies you'll meet sound adequate with their various battlecries and death screams.Each of the different types of enemies all have their own distinct sounds and that's definitely a plus in my book. Strangely,the bosses reuse some of the enemies' cries(although at a different pitch or tone).I'd understand if they were variations of the creatures themselves,but aren't bosses supposed to stand out?That's the whole point of being a boss,right?Recycling effects will make a boss stand out to be sure,just not in the way it's intended.
Sound rating:7/10
Summary:Needless to say,don't even bother picking up this game unless you're prepare to fight the controls as much as the enemies.Although the game looks and sounds good,the flaw of the controls drag it down to nearly unplayable.The lack of circle strafing also means lots of running back,stop,turn and shoot style tactics.
-Graphics are superb
-Sound quality excellent
-Weak storyline
-Uninspired characters
-Controls from the depths of hell itself!
-Mission objectives lack originality and diversity
Total score:4/10
Rating: 2.0 - Poor
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Gov. Jan Brewer threw a counterpunch at GOP lawmakers following the Monday release of their budget plan, ordering a halt to meetings between the two sides' budget staffs until the legislative budget plan is changed to address some of her key concerns.Read More »
House committee approves minimum tuition bill
A state House committee has approved a bill requiring full-time students pay generally $2,000 annually toward their tuition at a state university.Read More »
Cardon struggles to find a foothold
Cardon's been in the Senate race for six months, but it doesn't look like the needle has budged. A poll released today by Public Policy Polling shows Flake with a 56-7 lead among likely primary voters, and is virtually unchanged from November.Read More »
Feds identify 237,100 acres in Arizona for renewable energy projects
The Bureau of Land Management has recommended 237,100 acres of public land in Arizona are suitable for renewable energy development, part of an effort to speed up the process for clean-energy companies looking to set up shop in the state.Read More »
Mesa to be in national spotlight as it hosts GOP debate
In 2009, this city had so many foreclosures that President Barack Obama appeared at one of its high schools to speak about the national housing crisis. In 2011, a Mesa district recalled its state senator and anti-immigration leader Russell Pearce in a historic election. A month ago, Gov. Jan Brewer shook her finger at Obama at Phoenix-Mesa Gateway Airport.Read More »
Campus guns bill struggling in Senate
A proposal to allow guns on university and college campuses is stuck in the Senate.
The bill's chief sponsor, Sen. Ron Gould, R-Lake Havasu City, said he's so far only corralled 13 "yes" votes for the proposal.
Landrum Taylor's 14-year career was nearly over soon after it started
As Sen. Leah Landrum Taylor discusses her 14-year career in the House and Senate with pride in her accomplishments, she reveals that it all almost didn't happen. She nearly quit after her first term.Read More »
SRP's Greene shuns aggressiveness for solid arguments in lobbying Legislature
When Rep. Russ Jones moved into his office in the Arizona House of Representatives, his predecessor, Rep. Jim Carruthers, told him to beware of the "trains" that he could see, but perhaps would not hear coming.Read More »
'Parent empowerment' bill has roots in Democratic, Republican circles
The Goldwater Institute looked to a group of community organizers and Democratic operatives for inspiration for its latest school choice idea.
The institute helped draft and is advocating for SB1204, which would allow parents of students attending failing schools to close down the school, convert it to a charter school or remove the principal.
Uranium mining in the Grand Canyon region is unwise
I'm not an environmentalist and I don't understand all of the environmental issues, but I do understand that we get 5 million people coming to the Grand Canyon every year. Tourism is not compatible with mining or with the mining trucks transporting uranium to be processed in Utah, and generally running the risk of despoiling this national treasure.Read More »

subset: commoncrawl


text:
Submitted by JobUrban • February 24, 2014 www.joburban.com
IT part of India is one of the fastest growing industries which holds a major part of the economy of the country. Every company wants to grow and what can be the best place than Noida. Search your jobs in Noida and get the best.
- Category: Jobs
- Tags: job, jobs, jobs in noida


text:
Blessed are those whose strength is in you,
in whose heart are the highways to Zion.
As they go through the Valley of Baca
they make it a place of springs;
the early rain also covers it with pools.
They go from strength to strength;
each one appears before God in Zion.
Psalm 84:5-7
I remember one of the first times I read Psalm 84:5-7, I chided myself, "You don't go from 'strength to strength'; you go from 'worry to worry'.
You see, even as a youngster, I recognized the fact that I essentially "sought after" things to worry about. Once one worry didn't come to pass, I'd move on to the next one... In my early 20s, you could say I had legitimate concerns to worry me which ramped up my anxieties to a new fevered pitch. It was as if the only normal I knew was to dwell in the "Valley of Baca"; a valley I created by living from worry to worry instead of from strength to strength...
Where should my strength come from? Trusting in God whose strength provides spiritual rest as it carries me along on the highway to Zion...
But I must let Him...
Happy are those whose minds are wholly set on pilgrimage to Zion...
...those who in the strength of God, surmounting every obstacle,
appear in His Presence and offer their prayers.
Cambridge Bible for Schools and Colleges
I've definitely come a long way from my worry wort roots. You can read about those here.
But, that's not to say I still don't struggle with worry and anxiety...
The reality is, one of my biggest besetting sins is falling into the temptation of
When I look back at my almost 57 years of worry, I see all the time that was wasted worrying - because I'm still here!! You guessed it, my worries, more times than not, were focused on health concerns.
Since my 20s when my worries about health were for the most part unfounded, the Lord, in His lovingkindness, sent big tests in the area of my health... Creating even bigger opportunities to trust Him and kick temptation to the curb as I went from...
strength to strength...
A mystery illness that left me dizzy and incapacitated for six weeks; doctors unable to truly diagnose what was going on...
- Although I was practically debilitated from anxiety through this illness, the Lord provided a friend who was going through a similar phenomenon which encouraged me in a very discouraging situation
A baby step in trusting God as I experienced God's strength through the encouragement of a friend - strength I would need for the next time...
An unusual EKG that alarmed the doctor...
- It was then I felt my first sense of true peace in the midst of a scary finding. I remember handing the situation over to God right in the exam room and saying, "Lord you know exactly what is going on with my heart. This is too big for me to worry about, I leave it with you..." {It turned out my heart was just fine. In fact, the unusual EKG reading was a result of how God designed the tilt of my heart.}
The discovery of a thyroid nodule with the possibility of thyroid cancer...
- My immediate response was to storm the gates of Heaven, through the prayers of the Saints, for a clear biopsy. I believed God would answer, and He did!
All paving the way for the peace I would feel the next time...
And now we come to last week when I went for my routine dermatology full body skin exam. I was not concerned about anything, and as the doctor began her exam, she said she wasn't finding anything concerning either. Until she got to the very end of the exam, and I heard her say, "Hmm, what's this?". She said it is probably a lichenoid keratosis which will clear up with a six week course of cortisone cream. But then she said, or it could be a squamous cell carcinoma...
- I can honestly say that I stayed very upbeat and didn't even become alarmed. I even joked about it when my husband came home. I truly feel totally at peace about this.
The means by which the Lord used to strengthen me through the situations above, were certainly quite pivotal, but did not work alone. As I grew in God's strength to fight those particular temptations, I was also growing in the Lord, through daily Bible reading, Bible study, prayer and not forsaking gathering together with the body of Believers.
A firm foundation to stand on, and the Sword of the Spirit to employ.
Jesus set the example of how to effectively use of the Sword of the Spirit when fighting temptation...
Then Jesus was led up by the Spirit into the wilderness to be tempted by the devil. And after fasting forty days and forty nights, he was hungry. And the tempter came and said to him, "If you are the Son of God, command these stones to become loaves of bread."
But he answered, "It is written,
"'Man shall not live by bread alone,
but by every word that comes from the mouth of God.'"
Then the devil took him to the holy city and set him on the pinnacle of the temple and said to him, "If you are the Son of God, throw yourself down, for it is written, "'He will command his angels concerning you,' and "'On their hands they will bear you up,
lest you strike your foot against a stone.'"
Jesus said to him, "Again it is written, 'You shall not put the Lord your God to the test.'"
Again, the devil took him to a very high mountain and showed him all the
kingdoms of the world and their glory. And he said to him, "All these I will give you,
if you will fall down and worship me."
Then Jesus said to him, "Be gone, Satan! For it is written,
"'You shall worship the Lord your God
and him only shall you serve.'"
Then the devil left him, and behold, angels came and were ministering to him.
Matthew 4:1-11
When the Word of God is hidden in our hearts, we are able to discern the lies of the Devil when he tempts us, and to stand firmly on the Truth.
God promises that He is faithful, and He will not let you be tempted beyond your ability, but with the temptation he will also provide the way of escape, that you may be able to endure it.
1 Corinthians 10:13b
The Word of God directs our thoughts and actions in the ways of righteousness.
Your word is a lamp to my feet and a light to my path.
Psalm 119:105
The more we memorize Scripture, the more we know God. The more we know God, the more we love and trust Him. The more we love and trust God, the more of His strength we build up to break the temptation cycle.
...that your love may abound more and more, with knowledge and all discernment, so that you may approve what is excellent, and so be pure and blameless for the day of Christ,
Philippians 1:9-10
You might say, but Karen I am in the Word of God daily and I have memorized Scripture, but I still seem to fall short and succumb to the same temptation over and over again. I would respond, I do too...
But there is great encouragement to be found for our dilemma in the following excerpt from Streams in the Desert...
Temptation is necessary to settle and confirm us in the spiritual life.
It is like the fire which burns in the colors of mineral painting, or like winds that cause
the mighty cedars of the mountain to strike more deeply into the soil.
Our spiritual conflicts are among our choicest blessings,
and our great adversary is used to train us for his ultimate defeat...
So temptation victoriously met doubles our spiritual strength and equipment.
Streams in the Desert
"Every temptation victoriously met doubles our spiritual strength and equipment!"
Think about a diet. In the beginning it is hard not to be tempted to have sugary junk food, but we persevere keeping our eye on the goal of reaching a smaller size once again. It certainly is hard at first and can be down right uncomfortable when the hunger pangs hit us, especially at night if you're like me, but each time we resist the temptation to snack on junk food, it inevitably gets easier and easier to pass up that sweet treat to the point that there isn't a struggle anymore, but almost a resolve not to have desert - you have lost your desire for excessively sweet food.
We have the strength to fight the temptation to eat sweets through the establishment of a new habit; a good habit!
Why do I bring up the example of a diet? Because when we fall into the same temptation repeatedly, we have formed a sin habit.
Wikipedia describes a habit as "a routine of behavior that is repeated regularly and tends to occur subconsciously.[1][2][3]
"In the American Journal of Psychology (1903) it is defined in this way: "A habit, from the standpoint of psychology, is a more or less fixed way of thinking, willing, or feeling acquired through previous repetition of a mental experience."[4] Habitual behavior often goes unnoticed in persons exhibiting it, because a person does not need to engage in self-analysis when undertaking routine tasks. Habits are sometimes compulsory.[3][5] The process by which new behaviours become automatic is habit formation. Old habits are hard to break and new habits are hard to form because the behavioural patterns we repeat are imprinted in our neural pathways,[6] but it is possible to form new habits through repetition.[7]
As the habit is forming, it can be analysed in three parts: the cue, the behavior, and the reward. The cue is the thing that causes the habit to come about, the trigger of the habitual behavior. This could be anything that one's mind associates with that habit and one will automatically let a habit come to the surface. The behavior is the actual habit that one exhibits, and the reward, a positive feeling, therefore continues the "habit loop".[13] A habit may initially be triggered by a goal, but over time that goal becomes less necessary and the habit becomes more automatic."
I found this explanation quite sobering applied to the context of temptation to sin.
Instead of the temporary feel good sensation of sin which causes us to continually fall into the same temptation, let us make Jesus our reward and turn our sin habit into a Jesus habit. Every time temptation knocks at our door, we must pray and ask God to bring to our remembrance the very Truth that banishes Satan's lie. Each time we say no to temptation, we will become stronger for the next time, until finally, the temptation no longer has power over us.
Let us break the temptation cycle and go from strength to strength.
The strength of a man
consists in finding out
the way in which God is going,
and going in that way too.
~ Henry Ward Beecher
You might find me on these link-ups:
Grace & Truth Link-up, Woman to Woman Ministries, Growing in Grace Link-up, 3DLessonsLif4e, Inspire Me Monday Titus 2 Tuesday, A Wise Women Builds Her Home, A Look at the Book, Sunday Stillness, The Weekend Brew, Spiritual Sundays, Soul Survival, Give Me Grace, Testimony Tuesday, Women with Intention Wednesdays, Still Saturday, Good Morning Mondays, Tell His Story, Faith & Fellowship Blog Hop, Motivate and Rejuvenate Mondays, Be Salty, Monday's Musings, The Art of Home-Making Monday, Tuesday Talk, Waiting on Wednesday, Reflect His Love and Glory Words of Comfort Link-up, Wholehearted Wednesdays. Tell it to Me Tuesday, Faithful at Home Fridays, Faith 'N Friends, TGI Saturdays, Sitting Among Friends Encourage Me Monday Words with Winter Essential Fridays, So Much at Home Family, Friendship & Faith, House to Home ,Wonderful Wednesday , Monday of Many Blessings, Literacy Musings Mondays,Imparting Grace, SHINE Blog Hop Dance with Jesus, Give Me Grace, Booknificent Thursdays UNITE Cozy Reading Spot, Making Your Home Sing Monday Social Butterfly Sundays Let Us Grow Small Wonder Link-up Winsome Wednesday Growing in Grace Thursday After My Coffee, Rewind Friday Fresh Market Friday Moments of Hope Wonderful Wednesdays Christian Blogger Link-Up Tea & Word Tuesday Heart Encouragement Thursday Hearts for Home


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All quotes tagged So True
(2738 quotes found)
"A day will come where you won't be alone anymore. When you will finally find that one person you've been looking for all this time. That one person that means more to you that opens their heart and soul to you and is meant to last from this day forward. The one that will make you happy and loves you with everything they have. Stay strong and don't give up. I promise you that day will come."
"When you said forever, you meant a few months. When I said forever, I meant every day until I died. When you said always, you meant until you couldn't handle it anymore. When I said always, I meant until time ended. When you said you loved me, you meant I was no different from any other guy. When I said I loved you, I meant I had never felt what I felt for you."
"You did something to me, I don't know what it was but I do know that I don't want to lose you. You always keep a smile upon my face and I can act like myself because you accept me. You mean so much to me, the way you make me feel is just unexplainable. This is the happiest I've been in a awhile and I have to thank you for that. You just make it so easy to fall for you and believe me, I'm falling harder with each passing day."
"Even if it's true, it's three guys and unfortunately, this is going to be a label the team is going to carry."
"Updating yourself has never been so easy, but this fact holds true as always, you are already outdated."
"Be the architect of your dreams. Be the Michelangelo of your life. Go after what you want, make you dreams come true. And if it doesn't exist, go out there, design it and make it happen! But don't settle down, till you have reach what you came here for!..."


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This post was sponsored by Nature Made® as a part of a Blog Blast for Influence Central.
Staying on top of health and fitness is important, especially in my career as a web designer and blogger, I tend to sit at my computer way too much and forget the outside world exists sometimes! Well, that was last year, this year I have been getting out a lot more and making sure that I do. There is nothing more important than health, well being and making time for yourself, even when you have kids and work way too much!
Drinking Water
I used to drink water like a fish when I was a kid, no joke. When I started getting older, that turned into soda, coffee and much less water. This year I have been making sure I get at least half a gallon a day, I have noticed a difference in my skin and generally just feeling more hydrated. Water is so important and it's often forgotten, especially when you are busy, you forget to drink water and look after yourself, set an alert on your phone to drink a glass of water every hour or two during the day!
This really goes without saying, exercise does you the world of good, to some people that may be something that you dread, I used to feel like that. Now I get up and go to the gym once a day and it not only makes me feel better about myself, it releases stress, tension and makes me feel accomplished at the end of it. I am lucky enough to have someone to watch the kids whilst I go to the gym, but even if that's not an option, take the stroller outside and go for a 30 minute walk around the neighborhood every day, it's worth it!
Get your Vitamins
I used to feel like it was impossible to get my daily amount of recommended vitamins through food alone and it might well be, because that's a lot of food to intake! I now take Nature Made® Vitamins on a daily basis and I find that these help me immensely. Getting your daily nutrition is important for your overall health and I feel like I am making better progress at meeting those goals whilst taking Nature Made®.
Nature Made® is the nation's #1 best-selling brand for quality vitamins, minerals and supplements* to help support nutritional gaps and is the is the number 1 Pharmacist recommended supplement brand in 9 categories.
Get outside!
Fresh air will do you the world of good, even if it's not delightfully sunny or you don't live near a beach, just go outside for a while and take in some fresh air. Whenever I am feeling not too great, I step outside and it helps a lot. It helps me refresh and clear my mind.
Eat Healthier
Last year I ate way too much junk food and this year I have actually stuck to my goals, I've been juicing for the past few weeks, I have been eating healthier since January and I feel a heck of a lot better for it! Now, I am no angel, I have had a few bad days but I always try and remind myself why I am doing this, it's to be healthier, I ask myself, do I want to feel horrible after I eat this burger? Or do I want to stay feeling great right now and eat something healthy?
Keep Track of your Goals
Nature Made has a great Daily Boost Program and can give you lots of inspiration! Nature Made® products can be purchased at Walmart, it's a great destination to purchase Nature Made vitamins, minerals and supplements at great prices!
Join me at the Nature Made Total Wellness Twitter Party
Date: Tuesday, April 5th at 1 – 2 pm ET
Hashtag: #NatureMade
Moderator: @MomCentral
Focus: We'll chat about the new health routines we have built in 2016 and our goal towards total wellness. Whether taking regular yoga classes or spending time relaxing and reading a book, your total wellness focuses on the body and the mind. Nature Made's Daily Boost program is here to help give inspiration and keep you going with your goals!
Prizes: 2 pre-party winners and 6 party winners will receive a $50 Walmart gift card.
Giveaway – Ends April 12th
I-C is hosting a contest and you can enter to win one of 20 $50 Walmart gift cards!
Nature Made Wellness 5*I-C will randomly select 20 winners from all program entries and will handle fulfillment of the winning prizes. **Based on US News & World Report – Pharmacy Times Survey, Nature Made is the #1 Pharmacist Recommended Brand in Nine Categories – Letter Vitamins, Omega-3/Fish Oil, Coenzyme Q10, Flaxseed Oil, Herbal Supplements, Cholesterol Management-Natural, Garlic, Diabetic Multivitamins and Mood Health Supplements *Based in part on data reported by Nielsen through its Scantrack Service for the Vitamins Category for 52-week period ending 1/02/2016, for the xAOC Market. Copyright © 2016 Nielsen.


text:
Is it ok to just stop looking for a girlfriend
Most women already have boyfriends already and I'm horrible at reading signs for example had a girl last semester run into me on campus she said she was happy to see me and gave me Tim
Hortons, this was just being friendly but I took it as interest
Most Helpful Girl
It is ok to stop looking, but it is not ok to give up (as in, to have the giving-up attitude). If something good comes along, take it :)00|00|0
Why not give up a girl isn't entitled to me
hey thanks for choosing this as MHO :) no one is entitled to anyone, lol :)) that is not how you are supposed to look at it. You also benefit from a healthy functional relationship, not just her, and I think it is worth leaving a little window open for that possibility one you have closed all other doors :) just my opinion.


text:
Starcraft (okay, we made that last one up) plans to invest ₩80 billion ($64 million) in serious games. The Korea IT Times reports that the ministry is trying to grow the market to ₩500 billion ($400 million) by 2012. Minister Yu In-chon notes that the serious games market is at an early stage and is an "emerging blue ocean." Oh, look who's jumping on the hot topic business bandwagon.
The "Blue Ocean Strategy" is a concept where a company sets up shop in an unknown space, rather than compete in a cluttered market. For gamers, obvious examples of applied Blue Ocean Strategy would be Nintendo's entire marketing of the Wii, and developers who jumped on the iPhone app store. Currently, Korea has no specific serious games projects announced, but likely applications are based in the medical, educational and military fields.
[Via GI.biz]


text:
Hedge Fund Creator Decries SEC's Attempt At D&O Ban
The U.S. Securities and Exchange Commission wants to punish Patrick G. Rooney — who allegedly didn't inform investors that he had used $3.6 million from his Solaris Opportunity Fund LP to prop up his struggling microcap company, Positron Corp. — with a director-and-officer bar....
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Aloe Heat Lotion
When we're sick, a common symptom can be sore, achy muscles. Rather than endure the discomfort, why not ease it with Aloe Heat Lotion?
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An ideal massage lotion, it also provides soothing relief to tired, aching muscles. Aloe Heat Lotion is an emollient, oil-in-water lotion that contains warming agents, making it the best possible massage companion to relax your strained, aching muscles. It is also excellent for hydrating dry skin. Plus, it contains aloe vera, which assists the deep, penetrating power of Aloe Heat Lotion to bring you fast relief whenever your muscles need it! So be proactive when it comes to your health. Strive for winter wellness, and reach for the products that you trust the most!!!
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My husband is a goal keeper and he's been using Aloe Heat Lotion every time he plays soccer. Aloe Heat lotion is an excellent sports crème before and after exercise. Heat lotion will help stimulate healthy blood flow. He's been also using it if he has a headache, simple massage it on the forehead and a few minutes later he has no more headache.
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- Youth Restoring Forever Marine Mask - May 26, 2016
- Soothe away your Cares with Aloe Bath Gelee! - May 25, 2016
- Million's Favorite Product Over 38 Years: Aloe Vera Gelly - May 12, 2016


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I'm not really into white guys especially with blond hair and blue eyes but my boyfriend is like that snd I am very attracted to him and I thinkhe is really cute. So do you find it attractive also?
Blond hair and blue eyes, is it attractive?
Have an opinion?
What Guys Said 3
No I usually don't like blond hair. It makes them look dumb but there are very pretty (and smart) blond girls out there. Still not my usual preference.00|00|0
Proof positive that white guys aren't attractive to girls anymore, and lighter hair is the lowest on the totem pole. Sucks for them.10|11|0
Keep telling yourself that
White guys can also have dark hair
They're still considered the cream of the crop, especially in third world countries
Learn to read. I didn't say that white guys couldn't have dark hair. I said that white guys aren't attractive to girls, which is true. I don't believe your third world country comment. I don't anyone considers girls from those places to be cream of the crop. We all know who is considered to be that.
And I said that lighter hair is the lowest on the totem pole, which means that they are even less attractive to women than other white males.
What Girls Said 5
That is least attractive guy for me but I can still find some attractive.
I am not a huge fan of blue eyes and I think blond hair is the least attractive of all colors(for me, I know it's some peoples favorite).
Well unless you have long blonde hair...lol that always made me look twice :p
If you are attracted to him that is all that matters.
I didn't think it was attractive either until I met him haha.
I'm usually attracted to guys with dark hair , but I've seen some guys with blond hair and blue eyes and I found them attractive.00|00|0
but I prefer dark hair like black with brown/blue or green eyes
+3 - good question00|00|0
In guy form? Not to me.00|00|0
Yes beautiful00|00|0
Learn more
Select as Most Helpful Opinion?
You cannot undo this action. The opinion owner is going to be notified and earn 7 XPER points.


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There are several things to keep in mind when preparing for the summer months ahead. Here are some tips from the Beach Bum's "Pros":
- BRING WATER! You are going to playing for hours, it's very important to stay hydrated!
- Pack sunscreen. And reapply. Lots.
- Lots people like to play in sand socks, it helps keep your feet from burning/freezing, a great investment for year-round play. See our "GEAR" page for links.
- Bring an extra set of clothes. You may end up wanting to change for the after party or even half way through the day if you are playing hard. Come prepared!
- Speaking of clothes, many of us girls aren't sure what to wear to play in in the summer. Most girls are playing in bikinis during the day. Its much cooler, easy to rinse off in, and hey, you can work on your tan
- It's pretty well known that we do a charity raffle each month. 100% of that money goes to a local person or charity in need, BRING CASH to donate. Even if it's $1, every cent helps! Through these raffles we have helped raise over $6000 since we started, a little over a year ago.


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Article Side
Online Article Directory!
Use of Biological Samples in Medical Research
The science of human body is very complicated. People across the world suffer from different diseases and astonishingly the symptoms, diagnosis and treatment of many of them are yet to be discovered. Hence medical research plays a key role in developing medicines, formulating different methods of treatment and predicting the symptoms for diseases. In order to conduct this research human specimens are required. Research of human biological samples constitutes blood, urine, tissues, organs, hair, nail clippings, or any other cells or fluids. These samples are obtained from healthy volunteers or from patients who have expired.
Donation of such samples lends a helping hand for scientists to carry out their research work but at certain times, ethical and practical considerations may cause difference depending on circumstances. Blood samples possess the risk of bacterial infection at the sampling site and hence care and precaution must be strictly adhered to. Cleaning the site prior to sampling is very much necessary for this reason. The biological sample bank aims at storing different types of biological specimens at low temperature for future research in the fields of genetics, biochemistry and molecular biochemistry and is managed in accordance with international recommendations and ethical guidelines.
To avoid wastage of biological material after unfreezing, the samples are stored into aliquots of small volume, which can be used for different laboratory analyses. biological samples are usually processed using materials resistant to low temperature in order to prevent them from outside contamination. Samples are them stored into liquid nitrogen containers. Specially installed softwares keep track of all the stored samples and their location in the containers to match the biological bank information with the data bank information. The samples which are stored can be used for the purpose of research only on donor's consent.
James Blee - About Author:
For more information on biological samples, check out the info available online; these will help you learn to find the biological samples!
Article Source:
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text: Helloooo! I am a huge fan of eating...I know...NOT GOOD. LOL I had greek food for the first time the other week and looooved the hummus they had there!!! I went grocery last nite and picked up 2 containers of hummus... So I was wondering...WHAT CAN U DIP IN HUMMUS? ush: :shame: Please do share!


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I apologized for not writing as much as I had been doing. The month of May came and all of a sudden - Life happens. Everybody hibernating in the winter months are now very awake and out partying. The following projects kept me busy for the month of May.
Thank you very much for all the orders. Greatly appreciated and I hope you guys love them.
These musical soaps were giveaways from a music therapist to her students.
Pink cupcakes with butterflies were a little girls birthday giveaways for her classmates.
These full size cupcakes in yellow and black are graduation party giveaways. They travelled all the way to Missouri.
Butterflies for a 90 year old birthday party giveaways.
Graduation open house giveaways.
A nephew and a friend graduated college this year.
These are mini cupcakes.
.Hope everybody who received these giveaways enjoy the soaps. Again, thank you very much.
Congratulations to all the graduates!
Congratulations to all the graduates!


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To make the conference a success, WBENC relies on the support of capable volunteers. Those who work a four hour shift will receive a free Four Hour Pass to attend the conference and network with dynamic entrepreneurs as part of an event with tremendous economic growth impact.
Volunteer shifts are four hours in length. Some of the volunteer assignments include:
- Greeting and directing attendees to Conference activities
- Assisting at Conference Registration, or distributing conference bags and tee shirts
- Assisting with the Silent Auction or other evening program activities
To learn more about volunteering and what to expect, please review the Volunteer FAQs.
Volunteer Registration
Online registration is now closed, however, you can still volunteer by contacting:
Allison Gibson, Volunteer Coordinator
(626) 219-5360


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Philippians 3:14, I press on toward the goal to win the prize for which God has called me heavenward in Christ Jesus. Pressing on is a learned behavior! Amazing stories have unfolded in Sochi as athletes who have spent the majority of their lives training to press on towards the prize in the specific competition for which they have earned a spot. To see men and women collapse at the finish line in total exhaustion, having left nothing on the table, have triggered within us the emotions that accompany the human struggle. Be encouraged today that God is with you in your pressing on, in times good and times tough God is there in the presence of the Holy Spirit if you will only take his hand and by faith follow the Lord as a disciple focused on God's glory.
The good news of the gospel of Jesus Christ is that God is with us in our struggle and one day we will indeed by faith in Christ land on the platform on victory. Better than a gold medal we will receive the crown on life and glory forever and hear these words, "Well done good and faithful servant, come and enter my rest." Spending our lives like fuel for a cause is how we are wired by divine design, but that can only be accomplished as we surrender our strength and plan, and then receive God's strength and plan in an authentic relationship with Jesus Christ as Lord of our lives. God bless you today in your pressing on, know that the prize is guaranteed through the cross for you.
Press on!


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Thank you for your interest. REGISTRATION IS FULL for this year, but we look forward to seeing you next year!
Send your kids on an imaginative, impacting adventure this summer! Children ages 3 through rising 5th grade are invited to join us for Maker Fun Factory, the week of July 17-21, from 6:30-8:30 p.m.


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Monday evening was cold and dark as I drove to town bearing fresh sage, dried cherries and small onions. I was early because Darren and I were doing the main course that night, Roasted Duck with Cherry Madeira Reduction, and we had to get a head start if we wanted dinner before 9 p.m.!
We chopped and stuffed and got that baby in the oven and within 45 minutes the house smelled divine.
Kat arrived with a bag of oranges and a big ol' bag of whole cloves for us to decorate them with. I've always wanted to do that, but never had the chance. Even the guys got into the holiday spirit and we had grand fun clustered around the new dining room table (thanks, Jon!!) visiting happily as we attempted to make recognizable designs on our respective oranges.
Don made the evening truly festive with the best cocktail I've had in ages: a divine concoction of Coffee Liqueur, Vodka and cold milk. Delicious!!
At last the duck was ready and everyone gathered in the kitchen to fill their plates with Cameron and Robin's sprightly Spinach Salad with Mandarins and Almonds…
Jon's fabulous mashed potatoes infused with cumin, mustard, and pistachio mustard sauce…
Kat's savory Spanish Rice studded with peppers and herbs…
And the succulent duck that was tender and flavorful with marvelously crispy skin. I'm not usually a huge fan of duck, but this dish rocked my world. 🙂
Hope you have a lovely day! 🙂
Robin and Cameron's Spinach Salad
1 bag spinach
1 can mandarin orange segments or 1 mandarin orange, segmented
1/2 cup toasted, slivered almonds
1 part olive oil
1 part balsamic vinegar
herbes de Provence
splash of milk
- Place spinach, mandarin orange segments and almonds in bowl.
- In small bowl wisk together olive oil, vinegar, herbes de Provence and milk until emulsified.
- Toss with spinach and serve immediately.
Kat's Spanish Rice
(From Epicurious)
1/4 cup olive oil
2 onions, finely chopped
2 green bell peppers, seeded, ribs removed, and diced
3 cloves garlic, minced
1 cup long-grain white rice
One 8-ounce can tomato sauce
2 cups water
1 teaspoon celery salt
1/4 teaspoon black pepper
1 teaspoon cumin (optional)
- Heat the olive oil in a large frying pan over medium heat, then add the onions, green peppers, and celery and sauté until soft, about 3 minutes.
- Add the garlic and cook for another minute while stirring.
- Add the rice and stir together to mix.
- Add the tomato sauce and the water carefully.
- Season with the salt and pepper, and add the cumin, if using.
- Bring to a boil, lower the heat, cover, and simmer for 20 to 25 minutes, until the rice is done, stirring once or twice.
Jon's Mashed Potatoes
6 potatoes
1 onion, chopped
olive oil
2 Tbsp pistachio yogurt sauce
2 Tbsp creme fraiche
2 tsp cumin
salt to taste
1 Tbsp garlic powder
1/2 tsp mustard powder
1 tsp dried cilantro
2 Tbsp butter
1/2 cup milk
- Place potatoes in saucepan, cover with salted water, bring to boil, reduce heat slightly and cook 15-20 minutes until tender.
- Saute onion in olive oil until transparent and soft.
- Drain potatoes, add all remaining ingredients and blend together until they reach mashed potato consistency. Serve warm.
Darren and Krista's Roast Duck with Cherry Madeira Reduction
1 duck, washed, dried, innards removed
1 small onion or shallot, minced
2 Tbsp butter
2 onions, cut in quarters
handful fresh sage leaves
1.5-2 cups dried cherries
1 cup madeira or port wine
salt and pepper
- Preheat oven to 350 degrees F.
- Set roasting rack/cooling rack in roasting pan, place duck on top and set aside.
- In saucepan melt butter and saute onion until translucent. Add 1/2 cup wine and cook 1-2 minutes until mixture reduces and becomes saucy instead of watery.
- Stuff cavity of duck with quartered onions, sage leaves and 1/4 cup cherries. Return to roasting pan.
- Brush with onion wine sauce, sprinkle with salt and pepper, and roast for 3 hours, basting occasionally.
- Remove from oven and place duck on serving platter and let rest while you make sauce.
- Set roasting pan with juices on burner, add remaining wine and cherries, bring to boil, stirring constantly to scrape bits off pan. Let simmer until liquid reduces and cherries are soft.
- Slice duck and drizzle with Cherry Madeira Reduction.


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For his first feature film in America, we think Keiynan Londsale is doing alright.
The Australian native has always been a natural when it came to performing, with dance as his first love. Since then he's picked up more talents like singing, writing and acting. Keiynan's U.S. feature debut will be the highly-anticipated film Insurgent, in which he takes on the roll of Uriah, who is also a divergent.
C: How was your auditioning process? Did you fly to the states for it?
KL: "I did it on self tape back in Australia. It was pretty lucky that it worked that well. I sent my original tape and about a month later they said they wanted me to Skype with the director and casting director and I was lucky enough to get the call that I got the role. I was over the moon!"
C: The Divergent trilogy already had success and a big following by the time you got on board. Did you feel extra pressure being in this movie?
KL: "No, not necessarily. I was just excited to step onto a project with such a strong cast. I was a fan of the original movie. If anything I felt a little bit of pressure doing him [Uriah] justice because I knew fans would be really excited to see him in the second installment."
C: Tell us a little more about Uriah. What's your favorite thing about him?
KL: "Uriah is also a divergent, so that's why he and Tris develop a really great friendship where they get to share this trait about themselves. He's not just a fighter or the funny guy, he's also the guy who's kind of a deep thinker, even if he doesn't like to admit it. He just sees the world in a different light than most people. He's a good guy, a bit of a charmer, cool, confident and calm."
C: How was it working so closely with Shailene Woodley? As awesome as we'd imagine?
KL: "It's pretty amazing. She's got such a calm energy around her that makes you feel a little more confident and comfortable with what you're doing. My first scene was just me and her so I was pretty scared at first, but she's so great and her work on and off set is unbelievable."
C: Other than your accent training, what was the most challenging part of this experience?
KL: "I think it's just being comfortable in your own skin. For me, this was such a big project to walk into and it was so daunting to step into a world with such a big cast in a Hollywood film. I'm just not used to that world so it took me a while to find that air and energy to relax into it. It was hard to realize how confident I should be and just relax."
C: No spoilers, but will we be seeing Uriah in the next installment?
KL: "I believe so. You never know for sure, but he's definitely a big part of the last installment in the book. I want to continue to grow and I hope I get to do that. That's a great part of being a part of a franchise, you get to develop a character."
Our fingers are crossed for Uriah! Don't forget to check out Insurgent in theaters on March 20.


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brought to you by:
Q: What are the show dates and times?
A: Fall Atlanta Home Show & Outdoor Living Expo dates and times are as follows:
Friday, Sept. 8 from 10am-6pm
Saturday, Sept. 9 from 10am-6pm
Sunday, Sept. 10 from 11am-5pm
Q: Where is the show located?
A: The Fall Atlanta Home Show & Outdoor Living Expo is located at:
Cobb Galleria Centre
Two Galleria Parkway
Atlanta, GA 30339
24-hour information line: 770.798.1997
Cobb Galleria is located at the intersection of Cobb Parkway/US Hwy 41 and I-285, across from Cumberland Mall
Q: How much do tickets cost?
A: Ticket prices are as follows:
General Admission - $10
Children 12 and under - FREE
Military and First Responders BUY ONE GET ONE FREE
Senior Citizens 65+ with ID FREE
Multi-day pass $15
Be on the lookout for a $2 off coupon on the cover of the AJC courtesy of Window World!
Q: Where can I purchase tickets?
A: Tickets are now available to purchase online, and will also be available for purchase at the Show box office located at the top of the escalator on each day of the show.
Q: Is the Show handicap accessible?
A: Yes, there are elevators and wheelchairs are available.
Q: What can I expect at the Fall Atlanta Home Show & Outdoor Living Expo?
A: The Fall Atlanta Home Show & Outdoor Living Expo has over 300 exhibiting companies displaying a variety home improvement products and services including landscaping and lawn care, kitchen and bath fixtures, flooring, appliances, roofing, siding, concrete repair, windows and doors, heating and air conditioning, sunrooms and patios, insulation, and a whole lot more to save you time and money on your next remodeling project! Don't miss a wide range of topics on the Reliable Heating & Air Home Show stage presented by local and national experts too!
Q: Do I need to purchase a seperate ticket to gain access to the Outdoor Living Expo?
A: No, The Fall Atlanta Home Show and the Outdoor Living Expo are two great shows in one! Your General admission ticket will allow you into all of the exhibit halls at the Cobb Galleria Centre.
SEMCO Productions produced event.
Atlanta based and family owned since 1959.


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To 9th A EAS.
It's a game.
1.Enter and play .Use the key : PRINT SCREEN that copies what you see on your screen and paste it on a Word document. Describe it using the new words .Then send it to me to show the result.
If you prefer, you can leave it as a comment.
2. Optional. Design your own room and describe it. Use the same technique and send it to me.
You're going to have fun and get extra points.
3. I discovered another thing. If you play the game till the end, I receive your score in my e-mail !!! Fantastic. Agustina Bartolomé did it in 5.2 minutes. A good record. How long did you take?
A hug.
Your teacher
Sorry, it doesn't work anymore...I'll keep it anyway , to save my students' comments.


text:
Analysts spar over Flake's Stalin comparison
Former Trump spokesman Jason Miller and former Bill Clinton adviser Paul Begala argue over the comparison Sen. Flake (R-AZ) made of President Trump to dictator Stalin.
Illegal immigrant alleged cop killer wishes he…
Luis Enrique Monroy Bracamontes accused of killing two California police officers interrupts first day of trial with profane outbursts.
Truck veers off highway, crashes into school
The impact of the semi caused it to slam through the brick wall, stopping inside a classroom.
Prosecutors to announce charges in college…
Prosecutors plan to announce charges in the killing of a University of Pennsylvania student whose stabbed body found buried in a Southern California park
Threat of e-school closure adds to adversity for…
Students, parents and teachers from Ohio's massive Electronic Classroom of Tomorrow are considering backup plans in case the giant online charter school abruptly closes
Jury reaches verdict in drug smuggler's auto…
A jury has reached a verdict in an auto fraud case against a pilot who once flew loads of drugs for Colombian cartels during Miami's "cocaine cowboys" era in the 1980s
Pope denounces indigenous violence in symbolic…
Pope Francis has denounced the use of violence to achieve political gains in his visit to the heart of Chile's centuries-old conflict with indigenous peoples
Across the South, it's snow, ice and…
The South awoke Wednesday to a two-part Arctic mess: A blanket of snow and ice, and record-breaking temperatures
Million dollar idea: The zipper
The BBC's Aaron Heslehurst explains how the zipper became a million dollar idea.
The New York Times - Coming Soon: Conception
From The New York Times: There is no singular experience of becoming a mother. Our video series brings you first-person accounts of love, regret, doubt and strength.. Watch the original video on Times Video: https://nyti.ms/2ELQ7ic
A Sloth Was Rescued On A Beach In Costa Rica By A…
A lot of things wash up on beaches, and a sloth usually isn't one of them. Josh King has the story (@abridgetoland).
GOP senator denounces Trump attacks, recalls Stalin rhetoric
A Republican senator is denouncing President Donald Trump's use of the terms "fake news" and "enemy of the people" to describe the news media and stories he doesn't like
Teacher fired after telling student he'd be kicked out of US
A Maine substitute teacher has been fired after telling a student he would get kicked out of the country if the proposed U.S.-Mexico border wall is built.
Wall Street falls with oil prices
The Dow briefly surged above 26,000 for the first time Tuesday before lower oil prices dragged the index into the red. Fred Katayama reports.
Group pinpoints riches hidden in Europe's mountains of waste
Researchers have completed the first survey of valuable materials they say are waiting to be mined from Europe's vast landfills and scrapyards
Police: Man arrested for setting up spy cam in mall restroom
Police say they've arrested a man who set up a spy camera that recorded both children and adults in the family restroom of a Baltimore-area mall.
North And South Korea Will March Together At The…
After days of talks, North and South Korea have agreed to march together at the 2018 Winter Olympics under a unified flag.
Sen. Flake compares Trump's rhetoric to Stalin
Sen. Jeff Flake (R-AZ) condemns President Trump on the Senate floor, comparing the President's attacks on the media to the tactics of Soviet dictator Joseph Stalin.
Humans will review video from most popular YouTube creators
Google says humans will now review video from its most popular YouTube creators after recent complaints
Bannon to be interviewed by Mueller
Former White House chief strategist Steve Bannon is expected to cooperate and talk openly with special counsel Robert Mueller's team in lieu of testifying before a grand jury, a person familiar with the process said. CNN's Shimon Prokupecz reports.


text:
We are publishing this special update since there are so many special events and activities happening in Ixtapa Zihuatanejo during March! New events have been added!!!!
CARNAVAL ZIHUATANEJO! Started yesterday – Sunday, March 17 at Cancha Municipal (basketball court) in Zihuatanejo: We just verified with the Office of Visitors and Conventions that the activities will all be along the waterfront at Cancha Municipal (basketball court) in Zihuatanejo. The days and times of activities have changed since first announced. Enjoy!
- Today, Wednesday, March 13, 6:00 p.m.: "Carnaval Night" with an international ballet, clowns, singers and musical groups.
- Thursday, March 14, 6:00 p.m.: "Burning of Bad Humor and Opening Ceremonies officiated by Mayor Eric Fernández with ballet and music.
- Friday, March 15, 6:00 p.m.: "Coronation of the Queen of the Carnaval," international artists and dancing with an international ballet from the Gay Community
- Saturday, March 16, 6:00 p.m.: "Floats and Parades," through Zihuatanejo, then a ceremony at Cancha Municipal with international ballet and music. Mayor Eric Fernández is scheduled to participate at 9:00 p.m.
- Sunday, March 17, 8:00 p.m.: "Closing Ceremonies" with a ballet from "Casa de la Cultura" (Culture House), dancing and singing.
Saturday, March 16, 8:00 a.m., CLEAN THE BEACH in front of Azul Ixtapa Beach Resort. Let's keep our beaches clean! Come out and help clean and maintain our pristine beaches.
Monday, March 18, 2:00p.m. – 5:00 p.m., HANDMADE ART FAIR at "Enramada Rosita" Restaurant in Barra de Potosi. The Women's Co-op of Barra de Potosi invites you to come and enjoy the day in beautiful Barra de Potosi. Join them for snacks and shop for a handmade treasures at their Second Art Fair! Barra de Potosi is located about 20 minutes south of Zihuatanejo on Interstate 200. Go to the "Archotes" turn-off and proceed to the beach. "Enramada Rosita" Restaurant is located in Barra de Potosi, almost at the end of the road. Drive or stroll past the village and keep going around the curve along the front of the lagoon…there is lots of parking.
Monday, March 25 – Saturday, April 6, PETATLÁN FAIR: Live music, regional food, rodeo, Flying Men from Papantla, crafts, comedians, singers, dancing, flea market, cockfights, rides and many fun activities!
All Month, GERALD SHAW EXHIBITION OF WOOD CARVINGS at Galaria Coco Cabaña (next door to Coconut's Restaurant) in Zihuatanejo. A must to see! Visit the gallery during this month to see and purchase this spectacular collection. See our Culture Page: Gerald Shaw Exhibition of Wood Carvings Opens in Zihuatanejo | imagine-mexico.com.
All Month, Tuesday thru Friday, 6:00 p.m. to 8:00 p.m., ART, CULTURE AND TRADITION FESTIVAL: Enjoy spectacular shows, music, dancing, art, food and crafts at this annual festival. Admission is free!
- Tuesday and Thursday in Zihuatanejo at the Municipal Basketball Court (Cancha Municipal)
- Wednesday and Friday in Ixtapa on the main boulevard in across from Emporio Hotel
For more information about the Ixtapa Zihuatanejo area, see:
Local Attractions & Activities


text:
SEO Court Directory Recreation Sports
The Golden Baseball League is the premier independent minor league in North America. Teams are in Canada,Mexico,California,Arizona, and Utah. Major League players, college stars, and minor leaguers make up the rosters that play at a AA level. - [Read more]
Are you searching for high quality discount golf equipment? At our site, we assure you that we offer the best deals for your hobby. What kind of golf carts do you want? The golf bags we have – stand carry bags, cart bags, tour bags, and travel bags. Golf balls you can get – new, used, recycled, and Titleist deals. We have wedges, putters, chippers, and complete sets. So come to our site and learn how you can get the best golf equipment. - [Read more]
A huge collection of pictures of WWE Divas. Find information about your favorite WWE Divas. Watch WWE Diva Videos, and get wwe diva wallpapers from torrie wilson to stacy keibler. Watch wrestling matches of WWE Divas and interviews. - [Read more]
One of the leading adventure travel companies today is the Aurora Expeditions. The specialty of Aurora lies in its small team expeditions to the poles. Being family-owned, this company offers a friendly travel experience. They take it on themselves to enlightening tourists about these new territories. Their staff is experienced and has on board historians and naturalists as well. - [Read more]
Learn to Dance Videos, Exercise Videos, Fitness Videos and Sports Videos, DVDs and Books. - [Read more]
UK Parachuting are a sky diving drop zone based in Old Buckenham, Norfolk, UK. UK Parachuting specialise in tandem jumps for charity in the Norfolk and London area. In a tandem skydive you jump from 2 miles up and are securely harnessed to an experienced skydiving instructor. A tandem skydive is the ultimate gift for any jumper in Norfolk. - [Read more]
Quality corporate awards and sports trophies at great prices. Trophies, acrylic awards, crystal trophies, award plaques, wall plaques and more. Free engraving and friendly service. - [Read more]
Slane Cycles. Online bike shop supplying quality lightweight bikes and bike parts for racing and sports cyclists. - [Read more]
HorseRacing.co.uk is a horse racing news and betting portal catering specifically to British racing. All festivals, courses and race types are featured. - [Read more]
Paddlepro.com has been featured several times in Platform Tennis Magazine, and several leagues have their websites hosted on paddlepro.com's website, bringing Platform Tennis players to the site again and again. Paddlepro.com's homepage also offers daily news, dynamic content making this the greatest site for Platform Tennis. - [Read more]
Athlete Assessments has developed the only Sports Specific DISC Personality Assessments available today. To fully experience the benefits of Athlete Assessments, take your Athlete, Coach or Manager Online DISC Assessment now. - [Read more]
Offering golf holiday packages for Algarve Portugal. Package includes algarve golf tee times, car hire and hotel bookings. - [Read more]
NBA Blog and Information site featuring NBA news, standings, scores, stats, player and team information. - [Read more]
Our products cover the following: volleyball coaching and officiating equipment and many other volleyball products. If you want to be the best than shop at the best VolleyballProShop.com for all your volleyball equipment and volleyball gear. - [Read more]
A fan's site dedicated to the popular WWE Superstar John Cena. A collection of photographs, videos, clips of John Cena. Watch John Cena Videos. John Cena Pictures, John Cena Wallpapers. Watch the wwe superstar wrestle in the WWE Ring. - [Read more]
A fan's site dedicated to the popular WWE Diva Trish Stratus. A collection of photographs, videos, clips of trish stratus. Watch Trish Stratus Videos. Trish Stratus Pictures, Trish Stratus Wallpapers. Watch the wwe diva wrestle in the WWE Ring. - [Read more]
The Farmers Inn is a traditional 16th century hotel set in the heart of the Somerset countryside. We offer individually designed luxury rooms, restaurant and bar with � la carte menu and log fire. - [Read more]
Sports, pop culture, and attempts at humor - [Read more]
Offering a wide variety of products and gear for you to enhance yourself and your bike. - [Read more]
MMAstop your Source for MMA Fighters Gear, MMA Clothing and Fightwear from TapouT, UFC (Ultimate Fighting Championship) Clothing , mixed martial arts shorts. - [Read more]


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Sunday, June 26, 2005
Snappy Entry
Just came back from Sea World after spending a very hectic but thoroughly enjoyable day with Arnab (school time pal), Puja (Arnab's wonderful better half) and Amit (post-school friend). A million thanks to the wonderful couple who made me realize how wonderful love can be :) An equal number of thanks to Aunty (Vipul's mom) who made me realize what Indian food actually tastes like :)
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enjoyed a lot of fishy food, too? i thought people of regresive age went ther.... but the place looks mighty nice.
@sayantani - oh something i think i have mentioned on my blog earlier too - I don't eat fish :) and yes, the pace is really nice :)
@toe-knee - that's my line! that's my line :)
@toe-knee - that's my line! that's my line :)
"I don't eat fish :)" - Isnt that stmt kinda blasphemy? Especially since it originates from a Bong? I've known bongs who'd issue a fatwa and all for such remarks! :)
@sayantani - yes, i figured that from ur blog title and did find it quite fishy :)Post a Comment
@ajay - not just that - i also don't eat "sandesh" :(
@ajay - not just that - i also don't eat "sandesh" :(
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Any info if anyone is working on porting webOS for tge nexus 4?
Please share ur knolage
Sent from my Nexus 4 using Tapatalk 2
Please share ur knolage
Sent from my Nexus 4 using Tapatalk 2
I haven't kept up with WebOS development. It seems to have been abandoned. Which is such a shame for such an elegant OS.
Last I heard, WebOS was being ported to run on top of Linux, meaning anything Linux supports, it will support.
So if there was still any kind of activity behind WebOS, It would be trivial to port it, just like Jolla Sailfish, Ubuntu Touch and Firefox OS.
Sent from my Nexus 4 using xda premium
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Ryan Harris has snared a couple of wickets to help roll Kent for 280 on day three of Australia's opening Ashes tour game.
The visitors boasted a 227-run lead at lunch with the likes of Shane Watson and Mitch Marsh keen for some time in the middle in the afternoon.
Mitchell Johnson grabbed the headlines on Friday after terrorising the locals with pace and bounce in Canterbury.
"There is no way I get paid enough to be facing him – and I do alright for myself, don't get me wrong," Kent veteran Rob Key quipped.
Harris was relatively subdued but that changed when Kent resumed at 5-203 on Saturday.
The 35-year-old bowled tighter lines and was rewarded with the scalp of Sam Billings in his third over on day three.
Former England under-19 captain Adam Ball handed Harris another wicket soon after when he picked out Peter Siddle on the rope at fine leg.
"He can probably go up a notch," Key said of Harris after scoring 87 on day two.
"But he's quick. He's on you and he gets the ball to move probably more than the other Australian bowlers.
"The more he bowls, it won't be long before he finds rhythm. He's a very good bowler."
Harris, Australia's leading wicket-taker in the 2013 Ashes, is no certainty for the first Test in Cardiff given the form of incumbent pacemen Johnson, Mitchell Starc and Josh Hazlewood.
Mitchell Claydon was dropped on nine by Brad Haddin but added one run before Mitch Marsh completed a sharp caught-and-bowled chance to dismiss him.
Tailenders Matt Hunn and Adam Riley kept the visitors at bay for 10 overs but Johnson broke the partnership.
The left-armer made an instant impact when he started his second spell of the day, uprooting Riley's off stump.
Johnson finished with figures of 4-56 from 18 overs, having cranked up the pace on day two.
"I felt like I had dropped a little of pace throughout the West Indies and in the IPL," Johnson told Fox Sports.
"It wasn't too much of a concern for me.
"I was really excited about getting over here and playing in these conditions.
"I know the ball swings here in overcast conditions. It was nice to get out there and let a few rip."
Johnson's Ashes redemption came in 2013-14, when he snagged a staggering 37 wickets and terrorised England's batsmen.
However, the 33-year-old will be desperate to show he can do it in English conditions after a miserable tour in 2009.


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Scallops and bacon are a classic combination.
How can you go wrong with bacon anything?
The only thing that can top bacon in my book is prosciutto. That's exactly what I used to take this Prosciutto-Wrapped Scallops recipe to the next level. That, and truffle balsamic.
As you may have seen, Vegas and I are in Italy right now. It's my favorite place in the world, filled with my favorite food. Luckily we live right near the Little Italy neighborhood in San Diego, so we get a taste of heaven pretty regularly.
There is a massive weekly market in Little Italy where you can get local produce, peanut butter, live sea urchin… and fresh prosciutto.
For this recipe, I used my favorite Sizzlefish scallops. Vegas actually commented about how fresh they taste after taking a bite of this. They're flash frozen to ensure a fresh taste, then individually portioned… perfect for spur-of-the-moment diners like us!
Yes, this was a quickie dinner. It only looks fancy! I had a bit of the prosciutto left over and was looking for a good use for it. I'm not kidding when I tell you this was done in under 20 minutes.
If you're someone who's leery of searing scallops at home, don't be. They don't make a huge mess, and you can avoid over cooking them just by touch. If the scallops bounce back slightly, they're done. If they feel mushy, cook them longer. They also turn from translucent to opaque; just make sure to watch them closely.
One important tip for this recipe: make sure to dry the scallops off before wrapping them. It helps the buttery prosciutto stick to them better.
I served this with a parsley-arugula salad and cherry tomatoes. Any sort of greens would be a great compliment to these flavorful scallops!
Prosciutto-Wrapped Scallops with Balsamic
- 8 sea scallops (I used Sizzlefish)
- 8 slices of prosciutto
- 1 T olive oil
- 1 fresh lemon, halved
- Freshly ground pepper, to taste
- Balsamic reduction, to finish (I used this truffle balsamic)
Fold prosciutto slices in half lengthwise. Wrap one piece around the outside of each scallop, overlapping prosciutto ends and securing with a toothpick.
Heat pan to med-high and drizzle pan with the olive oil.
Sprinkle salt and pepper over each scallop, squeeze lemon over them, and place in pan. Cook 6-7 minutes, turning once, until just opaque. They are done when the prosciutto is a light brown crisp and the scallops are slightly golden.
Plate and drizzle with high-quality balsamic.
Makes 2 servings.
I wish I could bring a whole chunk of prosciutto back fro Italy. Guess I'll settle for a case of wine…
Have you ever made scallops at home?
Would you rather have bacon or prosciutto? Or vegan bacon?


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WilmerHale Latest To Abandon Lockstep
In a Tuesday memo the firm announced that the new system — which it says will be highly competitive and reflect individual skill level — will be implemented in all its U.S. offices over two to three years. The memo said the firm has not...
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Joe Danger The Movie to film next week
By News Division On October 6, 2012 · In News, Playstation 3, Retail, Systems
Hello Games this week said it will release Joe Danger: The Movie to the Playstation Network division of Sony Corp.'s Playstation 3 Oct. 9.
Joe Danger: The Movie for PSN will include 10 hours of exclusive content.
The title includes a total of six acts (levels) to allow the veteran stuntman to recreate action-based movie scenes from mine cart racing to skiing from an avalanche, or jet-packing to safety.
Follow us on Twitter @PunchJump, @WeLoveHotDeals, @PreOrderBonus, @Punchissimo, and visit us at Facebook and Google+.
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[Kartenspiels der berühmten Wahrsagerin Mlle Lenormand in Paris]
mid-to-late 19th century
by [German Fortune-Telling Cards]
B. Dondorf, mid-to-late 19th century. Ephemera. Very Good. Deck of 36 cards, complete; 86x53mm; full-color lithograph illustrations, beneath German text, Arabic numerals in upper-left corner, the manufacturer's seal and initials in upper-right corner; ornate design and manufacturer's initials on the verso. Some rubbing and very light dust-smudging, but still attractive. Scarce. (Inventory #: D14080)


text: Were having a bad snowstorm. Heard nothing if our day sort was running or not its a 30 min drive for me às im out in the country side. Got to work to only have a employee tell me no work its been sent to another hub and no supervisors came in... wtf thats crap. I risked my car being ditched me spinning out into someone just to go in and turn around Angry!!


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Accelerō® Bioanalytics - Good Laboratory Practice (GLP) Compliant
The Ligand Binding Assay
When pharmaceutical companies are developing new drugs, it has become a common practice to outsource the necessary bioanalytical work to Contract Research Organizations (CROs) that are Good Laboratory Practice (GLP) compliant, such as Accelerō® Bioanalytics. Ligand binding assay work represents one of the key quantitative methods in immunogenicity and biomarker studies for new microRNA therapies.
A ligand binding assay depends upon the binding of an analyte to an assay reagent, which facilitates the quantification of such macromolecules as proteins and olginucleotides in a given sample. While it is certainly possible for pharmacology firms to perform a ligand binding assay in-house, several factors make it more economical to outsource ligand binding assay work to an FDA Good Laboratory Practice (GLP) compliant CRO.
Outsourcing Ligand Binding Assay Work to a Good Laboratory Practice GLP Facility
Different levels of outsourcing are possible depending on the groundwork already done by the pharmaceutical concern. Ligand binding assay outsourcing may simply be a transfer of a firm's pre-developed procedure that a Good Laboratory Practice (GLP) compliant contract lab adopts and carries out according to the ligand binding assay client's specifications.
In other cases, the CRO may start with the pharmaceutical development company's protocol and optimize the ligand binding assay method to fit a different species matrix. The Good Laboratory Practice (GLP) mandates may cause a CRO to modify a client's ligand binding assay methodology to ensure compliance.
Another option for a drug development company that has built a working relationship with a Good Laboratory Practice (GLP) compliant contract research laboratory, such as Accelerō® Bioanalytics, is to entrust the entire ligand binding assay process to the expertise of lab personnel. Plenty of experience plus a track record of delivering accurate and well-documented ligand binding assay results, according to Good Laboratory Practice (GLP) standards, are two decisive factors in the client's decision to outsource a ligand binding assay series.
Advantages of Ligand Binding Assay Outsourcing
CROs can provide an on-call personnel forces for pharmaceutical concerns that do not have the resources to employ ligand binding assay technicians full-time. A Good Laboratory Practice (GLP) compliant contract lab, like Accelerō® Bioanalytics, has already invested the extra time and cost to ensure that every ligand binding assay it does for a client yields accurate, reproducible results, thus saving clients the expense of creating their own Good Laboratory Practice (GLP) facility.
Another important advantage of outsourcing ligand binding assay development, analysis and validation is that, by entrusting the work to an experienced, Good Laboratory Practice (GLP) CRO, a drug development firm can focus on those aspects of the bioanalytical process that staff members are well qualified to do. The end result will be more efficient development of microRNA and other therapies within a shorter time frame, and within the specifications of Good Laboratory Practice( GLP).
By establishing a strong working relationship with a Good Laboratory Practice (GLP) lab such as Accelerō® Bioanalytics, a drug development company is cultivating an important resource that can step in to provide backup support for any in-house lab backlogs as well as deliver the Good Laboratory Practice (GLP) data critical to moving forward towards drug approval.
The Ligand Binding Assay
A ligand is a substance that binds with a target biomolecule. In the Good Laboratory Practice (GLP) facility, a ligand serves as a signal triggering molecule that binds to a protein, revealing specific test results. Various ligand binding assay applications may utilize ligands that are substrates, inhibitors, activators, or neurotransmitters. Their binding property, or the strength of the bond formed, is called its affinity.
A ligand binding assay, carried out under the strict standards of Good Laboratory Practice (GLP), can determine the binding affinity between a ligand and its receptor molecule. The strength of the affinity becomes relevant when the energy created by a high affinity ligand bond can be directed toward causing a change in reception conformation, which alters the behavior of an associated enzyme.
A ligand that behaves in this way, changing its receptor and triggering a behavioral response, is called an agonist. By helping researchers to determine the level of affinity between a ligand and its receptor, a Good Laboratory Practice (GLP) compliant ligand binding assay reveals the optimum concentration of the agonist for triggering the intended physiological response, thus providing data important to determining dosages of microRNA drugs.
>A ligand binding assay can also determine ligand efficacy -- the biological effect and the magnitude of the effect when the ligand binds to its receptor. Efficacy is another critical factor in dosage determination. Only a Good Laboratory Practice (GLP) compliant CRO, such as Accelerō® Bioanalytics, is fully qualified to perform a ligand binding assay that provides the most accurate, reproducible, and meticulously tracked results.
By carrying out ligand binding assay support in an FDA certified, Good Laboratory Practice (GLP) environment, Accelerō® Bioanalytics produces indispensable, reproducible data for pharmacokinetic assessment purposes in the development of biomarkers and detection of immunogenicity, accelerating the progress of essential new drug therapies towards FDA approval.
Ligand Binding Assay Basics
Different types of the ligand binding assay performed in a Good Laboratory Practice (GLP) environment include the sandwich hybridization assay, ligation-based hybridization, and Real-Time RT-qPCR. With the exponential growth of nucleic acid therapies, many developers of pharmacokinetic and pharmacodynamic drugs order ligand binding assay work for microRNA and siRNA therapeutics.
Liquid phase ligand binding assay formats include Real-Time RT-qPCR and immunoprecipitation. Solid phase ligand binding assay formats include multiwall plate assays, on-column assays, on-bead assays, filter assays, and membrane assays. With several different formats that each are applicable to specific nucleic acid therapies, the importance of selecting a CRO experienced in all types of ligand binding assay formats, that is Good Laboratory Practice (GLP) compliant, become evident.
Recognizing the need for CROs compliant with Good Laboratory Practice (GLP) to carry out ligand binding assay procedures that focus on developing new nucleic acid drugs, Accelerō® Bioanalytics has devoted several years to developing this specialty. Accelerō® Bioanalytics provides the best possible Good Laboratory Practice (GLP) compliant ligand binding assay work for pharmaceutical research in this field of study.
In general, the CRO will perform the following steps in a ligand binding assay (2), although some Good Laboratory Practice (GLP) facilities, such as Accelerō® Bioanalytics, are able to customize a ligand binding assay to a client's specifications:
• Reagent assemblage – an experienced Good Laboratory Practice (GLP) facilities can create custom reagents upon request
• Assay format selection
• Optimization of experimental design
• Establishment of ligand binding assay parameters
• Testing of known agonists
• Validation of results
During the pre-study validation phase of the ligand binding assay, a Good Laboratory Practice (GLP) contract research lab will select the appropriate assay format from a variety of commercially-available kits, or will customize the ligand binding assay according to client requirements. Such considerations as the correct selection of reagents with the right stability and specificity for the assay, and the appropriate selection of fluorescent ligand marker labels can be critical to the success of the ligand binding assay.
During the ligand binding assay, validation as required by Good Laboratory Practice (GLP) must be ongoing to ensure that the methods selected for the study remain effective throughout the process. Good Laboratory Practice (GLP) activities, such as monitoring control samples to check the performance of the ligand binding assay, help to ensure high quality results.
1. De Jong,L.A., et. al. Receptor-ligand Binding Assays: Technologies and Applications. December 2005.
Available online at: http://www.ncbi.nlm.nih.gov/pubmed/16253574. 2011.
2. Assay Guidance Manual Version 5.0, 2008, Eli Lilly and Company and NIH Chemical Genomics Center.
Available online at: http://www.ncgc.nih.gov/guidance/manual_toc.html. 2008.
Applications for the Ligand Binding Assay
As a quantitative tool, the ligand binding assay centers around the binding of an analyte to an assay reagent. When it comes time to quantify macromolecules like oglinucleotides within a biological matrix, an organization will most likely turn over the ligand binding assay work to a Good Laboratory Practice (GLP) contract research lab such as Accelerō® Bioanalytics.
Before selecting the laboratory to carry out a ligand binding assay, a pharmaceutical development company should verify that, like Accelerō® Bioanalytics, the CRO is fully Good Laboratory Practices (GLP) compliant, ensuring the most accurate and verifiable ligand binding assay results. While many labs may offer the ligand binding assay in their portfolio of services, only a Good Laboratory Practices (GLP) facility complies with FDA standards to do such testing.
The ligand binding assay is often used to quantify specific biomarkers for pharmacodynamic or toxicokinetic purposes. A ligand binding assay may be used to study biomarkers for experimental therapies. Any data resulting from the study must be recorded in compliance with Good Laboratory Practice (GLP) guidelines to meet the highest standards for accuracy and reproducibility.
Macromolecule therapies designed to act against specific target proteins, or receptors, can be combined with one or more other drugs to present a united front against such multifaceted diseases as cancer. A ligand binding assay can measure the efficacy of protein-targeting therapies. The resulting data, subject to the meticulous record-keeping controls of the Good Laboratory Practice (GLP) compliant CRO, such as Accelerō® Bioanalytics, will help pharmaceutical developers evaluate the specificity and selectivity of an experimental nucleic acid therapy.
Good Laboratory Practice (GLP) compliant labs, such as Accelerō® Bioanalytics, are uniquely qualified to perform ligand binding assay studies, both from a Good Laboratory Practice (GLP) standpoint and also by virtue of their experienced staff of immunochemistry experts.
Radioactive and Non-radioactive Ligand Binding Assay
The receptor ligand binding assay determines the nature of receptor-ligand interactions in biological systems and provides valuable quantitative data, gleaned and recorded according to Good Laboratory Practice (GLP) standards, that can be crucial to pharmaceutical progress. The two basic receptor ligand binding assay formats available are radioactive and non-radioactive assays.
By nature, the radioactive ligand binding assay format can be hazardous, posing a danger to the health of the technicians in the laboratory as well as producing radioactive waste. While a lab may be Good Laboratory Practice (GLP) compliant, it does not necessarily possess the facilities to deal with the extra expense of a radioactive ligand binding assay format.
That is why, despite the accurate, reproducible results a radioactive ligand binding assay can produce, Good Laboratory Practice (GLP) CROs often utilize the safer, non-radioactive ligand binding assay format to deliver similarly reliable data.
The non-radioactive ligand binding assay involves the use of fluorescent polarization or fluorescence resonance energy transfer technology that optimizes the ability for the ligand binding assay technician to quantify results. A ligand binding assay that utilizes fluorescent markers is applicable to receptor ligand binding assay formats for the biological screening and quantification of pharmaceuticals (1).
The Importance of Good Laboratory Practice GLP
In a contract research facility, Good Laboratory Practice (GLP) outlines the standards for designing and implementing a ligand binding assay as well as reporting ligand binding assay results. Good Laboratory Practice (GLP) standards are not only applicable to a single study, such as a ligand binding assay, but help shape the infrastructure of the laboratory itself, informing all activities within the lab.
One reason Good Laboratory Practice (GLP) compliance is so important for reputable CROs, such as Accelerō® Bioanalytics, is that pharmaceutical clients can rely upon these standards to assure correct results that can be exactly reproduced in a duplicate ligand binding assay or other Good Laboratory Practice (GLP) study.
CROs like Accelerō® Bioanalytics, which are Good Laboratory Practice (GLP) compliant in all of the work they perform, adhere to the highest industry standards. Clients seeking the most professionally run CRO for the highest caliber of ligand binding assay and other testing results acknowledge the importance of Good Laboratory Practice (GLP) certification.
Elements of Good Laboratory Practice (GLP) Guidelines
Some of the standards set by Good Laboratory Practice (GLP) include:
• Establishment of written Standard Operating Procedures (SOP) within the facility
• Meticulous record keeping that stands the test of time
• Space and management divisions that ensure the integrity of each ligand binding assay or other study, eliminating the chance of cross-contamination
• High-quality instruments that are calibrated and well maintained
• Data retention standards that protect the integrity of test results
• Archive procedural guidelines
History of Good Laboratory Practice GLP
Prior to the mid-1970s, before the creation of Good Laboratory Practice (GLP) guidelines, the U.S. Food and Drug Administration relied on the sponsor – manufacturer – of a drug submitted for FDA approval to verify and present accurate, verifiable data as needed for the approval process. When whistle blowers called the test data of several labs into question, FDA laboratory inspections revealed falsification of results and defects in the design and reporting of studies meant to substantiate claims of candidate drug efficacy and safety.
The FDA established Good Laboratory Practice (GLP) regulations in 1976 as a result of these revelations. Several years later, the U.S. Environmental Protection agency adopted the Good Laboratory Practice (GLP) regulations to govern testing procedures for industrial and agricultural chemicals.
Enforcement of Good Laboratory Practice GLP
Once a CRO like Accelerō® Bioanalytics earns the Good Laboratory Practice (GLP) designation, it must maintain the stringent practices that the guidelines proscribe. The FDA routinely carries out bi-annual facility inspections to verify continued Good Laboratory Practice (GLP) compliance in each designated laboratory.
The FDA inspector identifies, notes and discusses deviations, if any, from Good Laboratory Practice (GLP) standards with laboratory personnel. The CRO must then design a corrective action plan. The FDA must approve this plan in order for the CRO to retain its Good Laboratory Practice (GLP) designation. This inspection procedure ensures uniform quality and compliance with FDA guidelines, assuring clients that the testing, such as a ligand binding assay, performed in a Good Laboratory Practice (GLP) facility, will be done under the highest possible standards.
Why Every CRO is Not a Good Laboratory Practice GLP Facility
Because a contract research organization, such as Accelerō® Bioanalytics, gains stature and a reputation for reliability when it is Good Laboratory Practice (GLP) compliant, pharmaceutical firms that need ligand binding assay work and other essential laboratory studies will actively seek it out. Therefore, it makes sense that all CROs would strive for the Good Laboratory Practice (GLP) designation.
The truth, however, is that earning a Good Laboratory Practice (GLP) certification is a time-consuming, meticulous process that puts a CRO to greater expense than a non-Good Laboratory Practice (GLP) compliant facility. More highly specialized training and divisions of responsibility among the staff in a Good Laboratory Practice (GLP) means more expense. Safeguarding against cross-contamination, according to Good Laboratory Practice (GLP), when performing more than one ligand binding assay simultaneously means additional laboratory space is necessary, requiring a more expensive facility.
When a contract lab, such as Accelerō® Bioanalytics, is willing to invest the time and money it takes to comply with Good Laboratory Practice (GLP) guidelines, that means that the staff values those ideals set forth by the FDA to ensure that they provide only the most accurate and reproducible data from a ligand binding assay or other microRNA study to pharmaceutical development firms. While the difficulties presented by Good Laboratory Practice (GLP) compliance may prove too exhaustive for some CROs to overcome, Accelerō® Bioanalytics goes the extra mile on a daily basis to comply with Good Laboratory Practice (GLP) protocols.
Putting the Pieces Together:
Good Laboratory Practice GLP and the Ligand Binding Assay
The laboratory analysis of a ligand binding assay is only one aspect of the bioanalytical process governed by Good Laboratory Practice (GLP). The pre-assay collection of the biological sample to be used in the procedure, as well as the post-assay documentation that supports the ligand binding assay are equally important.
While sample collection and processing is often done in off-site facilities, making it impossible for the Good Laboratory Practice (GLP) contract research organization to ensure uniformity of ligand binding assay materials, adherence to Good Laboratory Practice (GLP) procedures by other entities carrying out the processing ensures the uniformity of the result.
Good Laboratory Practice (GLP) standard guide the labeling, documentation, and storage of samples once they reach the CRO. Good Laboratory Practice (GLP) also proscribes the detailed record keeping that tracks and catalogs all ligand binding assay materials, eliminating the possibility of mix-ups and safeguarding the integrity of the supplies.
Each area of the Good Laboratory Practice (GLP) facility will have an SOP that informs receipt and handling of test items and equipment. Guidelines for laboratory operations for the ligand binding assay procedure as well as regulations for documentation ensure that, from receiving the supplies to recording the results, technicians complete all aspects of the ligand binding assay according to Good Laboratory Practice (GLP) standards.
Accelerō® Bioanalytics is a Good Laboratory Practice (GLP) facility with the experience and standards to undertake any type of ligand binding assay on a contract basis, providing the high quality, reproducible results critical to the drug approval process.
Accelerō® is a registered trademark of Accelero Bioanalytics, Berlin.
More details on bioanalytical ligand binding assays in drug development are kindly presented in our ligand binding assay album.


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Diamond Petroleum Introduces New Products to Thermal Line
Diamond Petroleum Ventures (DPV), a thermal unit provider based in Lafayette, La., has developed the Diamond TH 450 and TH 650 to take care of client thermal heating requirements, announced company President Rusty Lamb.
Both units have been designed to fit in tight spaces and are easy to transport. These units weigh between 13,800 and 16,500 pounds and have redundant fluid transfer pumps. As Class 1 Div. 2 compliant, these flameless heaters can heat 100 barrels of diesel 100 degrees over ambient in 80 and 60 minutes with no contact with corrosive exhaust gases.
Also available are the Diamond High Pressure Fluid Pump Skid and Thermal Tank System. The pump weighs 11,500 pounds, has a Gardner Denver TEE pump and was designed for use by a single operator. The Diamond Thermal Tank System weighs 9,500 pounds, has multi-directional valving and has a capacity of 88 barrels.


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Located on the shores of Silver Lake in Western New York, Asbury is a premier retreat center that offers a variety of facilities to meet everyones needs. Our site boasts over 60 acres and over a quarter mile of shoreline on Silver Lake. In addition to providing facilities for groups to conduct their own retreats, meetings, worship services, and programs, we can assist with program planning, guide groups through our low ropes course, conduct retreats and workshops, lead singing and games, and provide supervision for recreational activities.
Asbury also offers year round retreats for families, children and youth, clergy, and adults.
Our 2018 Retreat Registration will be open soon! To search all of our programs click HERE.
Come and see what Asbury has to offer. Meet the staff, sample our food service specialties, or take a swim in the pool. Experience the beautiful surroundings of Asbury as you enjoy a pontoon boat ride on Silver Lake or just relax in a rocker on the porch of our Manor. We can't wait to meet you! A delicious chicken BBQ dinner is available for purchase. Reservations encouraged, but not required. All ages welcome… MORE
The Fall edition of our newsletter, Xenia News, is now available online. Click here to read about what's been going… MORE
We want to hear from you. Help tell our story, by telling us yours. How did Asbury affect you or your family?Share Your Asbury Story