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Schools & Colleges
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We accelerate and catalyze the translation of innovative science into improved health and patient care.
The Colorado Clinical & Translational Sciences Institute (CCTSI) is the academic home to help transform the clinical and translational research and training efforts at the University of Colorado Anschutz Medical Campus and affiliated institutions. The CCTSI was created in 2008 with funding from the Clinical and Translational Science Award (CTSA) initiative of the National Institutes of Health (NIH).View our CCTSI funded grant.
NIH Funding Acknowledgment: Any publications, patents, or projects that benefit from any CCTSI resources (including use of REDCap) must credit the CTSA Grant by including the NIH Funding acknowledgment.
CCTSI is supported in part by Colorado CTSA Grant UL1 TR002535, KL2 TR002534, & TL1 TR002533 from NCATS/NIH. An Institute at the University of Colorado Denver | cctsi.ucdenver.edu | 720-848-7100
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June 13, 2005
LOS ANGELES - C.K. Prahalad, the Harvey C. Fruehauf Professor of Business Administration at the University of Michigan Ross School of Business, brought his vision of world class products for the world's poorest consumers to UCLA Anderson in his keynote address at the 2005 SABA Conference. Developing countries, he said, may leapfrog developed countries with new products and services since the challenges are so complex that they require radical innovation, and change is not stymied by legacy systems.
The best-selling author described India as a laboratory for innovation in which a large number of poor and uneducated people co-exist with a growing number of highly-skilled scientists and engineers. Jumping from illiteracy to cutting-edge knowledge work, he said, is what innovation is all about. Prahalad presented examples of radical innovation in India, inviting the audience to imagine modern hotel rooms for $20 a night, artificial limbs for $25, cataract surgery for $30 and other revolutionary products and services designed for those at the bottom of the economic pyramid (BOP).
Prahalad challenged the audience to see five billion people in developing countries, 80 percent of the world's population, as a market opportunity. "How do you serve a consumer market when all you can see is abject poverty," he asked. Seeing a market at the bottom of the pyramid, said Prahalad, requires imagination rather than analytics. "If you use the analytical tools we teach you," he said, "you will not see a market."
Opening the market at the bottom of the pyramid fundamentally challenges every assumption we have made about how to serve the world's richest societies, said Prahalad. Entrepreneurs and researchers, he said, must learn to marry low cost, good quality, sustainability and profitability at the same time. The result, he predicted, could be a market as transformative as the Internet.
Watch a video of C.K. Prahalad's keynote address (Windows Media).
indiOne Reinvents the Business Hotel
"Indians travel. That's a fact," said Prahalad who noted that India's hospitality industry is largely undeveloped. "It's like all of Scandinavia, and then some, on the move every day." Prahalad described the conceptual process that led to the creation of indiOne Hotels. "You can't take a five-star hotel and start taking things away. That just produces a budget hotel." Ground rules included a minimum room size, affordable pricing, modern features, and scalability up to 100,000 rooms. The first indiOne Hotel is open in Bangalore, India and ten more are expected to open in the coming year. Each will offer clean rooms with LCD TVs, business centers, exercise equipment, ATM machines and pleasant surroundings for about $20 per night. Indian consumers want world class quality at local prices, said Prahalad. The first indiOne Hotel was profitable within a month, and Prahalad calls it a model for global expansion.
India to Challenge Automakers in Developed Countries
India's auto industry is growing, said Prahalad, with most new cars priced from four to five thousand dollars. Prahalad noted that India's manufacturers, recipients of many quality awards, have learned to produce automobile components for a fraction of what they cost in the United States. "So then, I ask whether there is a market for a $3000 car," he says. Given the gap between a $5,000 new car and a $1000 motorcycle, the market is obvious, he said. "If you go from $5000 to $3000, that represents a five to six times price advantage over the United States," he said, noting that American cars start from $15,000 to $20,000. "If you were an American automobile marketer, would that bother you?" he asked.
And something else is happening, he said. The three areas in India where automobiles are made are the same locations where India's technology development is taking place. Prahalad suggested that India could revolutionize the automotive industry by combining automotive knowledge, manufacturing quality, small batch capability, low cost and embedded software.
Doing Well and Doing Good
Prahalad turned to the large number of people in India and the developing world who have lost arms or legs in war or to landmines. "If people can afford shoes, they cannot wear them in a temple or kitchen, so an artificial foot must look like a foot," he said. "A patient must be able to squat on the floor and walk on uneven ground, and you must be able to provide custom fitting for a patient in a single visit.
"Overall specifications for prosthetics in India are more stringent than in the United States. So, if prosthetics cost $12,000 in this country, how much should one be in India?" A Jaipur Foot, he said, costs about $25 in India. Prahalad again urged listeners to think about the opportunity for extraordinary innovation in serving the bottom of the pyramid.
"I want you to think about innovative, high-tech solutions at new price and performance levels," he said. "Not five percent less, but five percent of what it costs here. This is the sandbox that the bottom of the pyramid forces you to play in ... But you can't do this unless you have imagination, passion, courage, deep sense of humanity and humility. We can do well and do good at the same time."
About C.K. Prahalad
Prahalad is a best-selling author as well as an influential management professor and consultant. He is the Chairman and Founder of The Next Practice, a strategic advisory firm that enables the world's leading companies to leverage the emerging trends that are reshaping the face of competition.
Professor Prahalad's books include Competing for the Future (1994), co-authored with Gary Hamel. The Future of Competition: Co-Creating Unique Value with Customers (2004), co-authored with Venkatram Ramaswamy. The Fortune at the Bottom of the Pyramid: Eradicating Poverty through Profit (2004) was selected as one of the best books of the year 2004 by the Economist, Fast Company and Amazon.com.
He is a member of the United Nations Commission on Private Sector and Development. Professor Prahalad has consulted with the top management of many of the world's foremost companies, such as Ahlstrom, AT&T, Cargill, Citicorp, Eastman Chemical, Oracle, Phillips, Quantum, Revlon, Steelcase and Unilever. He serves on the Board of Directors of NCR Corporation, Hindustan Lever Limited and the World Resources Institute.
About UCLA Anderson School of Management
UCLA Anderson School of Management is perennially ranked among the top-tier business schools in the world. Award-winning faculty renowned for their research and teaching, highly selective admissions, successful alumni and world-class facilities combine to provide an extraordinary learning environment. UCLA Anderson students are part of a culture that values individual vision, intellectual discipline and a sense of teamwork and collegiality.
Established in 1935, UCLA Anderson School of Management provides management education to more than 1,400 students enrolled in MBA and doctoral programs, and some 2,000 executives and managers enrolled annually in executive education programs. Recognizing that the school offers unparalleled expertise in management education, the world's business community turns to UCLA Anderson School of Management as a center of influence for the ideas, innovations, strategies and talent that will shape the future.

subset: commoncrawl


text: Young adults are among the most likely not to have health insurance. The high costs of insurance are often a reason for that. Unfortunately, not having insurance can also be very expensive and leave you with medical debt. Whether you're a student, working or looking for work, there will be new options to buy insurance starting on October 1. You may even be eligible for financial aid to help lower your costs. Whether you're a young adult looking for insurance or a parent looking for your child, we'll cover your options.


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"Reduce excesses, but not excessively." Argisle
If you are concerned about your drinking habits, consider keeping a "drink
diary" for the next month. Write down when, how much, and in what
circumstances you drink. How does it make you feel immediately and the
morning after? Honestly look at how drinking affects your life. There are
many healthy ways to celebrate life! Playing sports does this, as can
drumming and dancing with your friends. While we used to have rituals, now
people get drunk to deal with life, and then get in their "deadly" autos
risking lives on their way. And many also use painkillers and other drugs to
deal with their daily lives, which further affects their abilities. Or they
overuse their credit cards and mismanage their lives and have their families
suffer. Realize that drug problems of all kinds are diseases, and should be
dealt with as such.
2. In evaluating how alcohol affects your life, look at the following
issues: Do you drink more when you're sad or upset about something? Do you
mainly drink in social situations with friends? Does alcohol help you
express yourself? How can you address these emotional issues without relying
on drinking? Can you say NO when the time is not right for your personal
imbibing? Healthy use would be a social drink or two with preparation before
and balance after in terms of hydration (alcohol is dehydrating) with water
and the right nutrients.
"No is On Backwards." (Argisle)
3. Consider the negative effects alcohol might be having on your health. Is
drinking aggravating an existing condition, such as obesity, digestive
problems, joint pains, or depression? Do hangovers affect your performance
at work, or keep you from things that you want to do? Alcohol irritates the
liver and the intestinal lining. It also lowers immunity, dehydrates the
body, and impairs judgement and performance, as in operating machinery and
driving a car. It also alters healthful participation in relationships.
Alcohol is the number cause of traffic fatalities, especially of innocent
people. What is your awareness and responsibility for your own body as an
example to self, loved ones and friends?
4. Alcohol may have some positive attributes as well. Doctors may recommend
it to reduce heart disease risks, as it helps raise the good HDL
cholesterol. However, it also raises total cholesterol and has more negative
effects such that health conscious practitioners would not recommend regular
consumption. Alcohol is also addictive. Thus, it takes some work, both
physically and emotionally, to heal from this potentially destructive habit.
For example, a liver transplant, if you could get it, is quite costly and
5. In terms of reducing your alcohol consumption, if you notice that you
tend to drink at certain times of the day, plan other activities, such as
exercising, during that time period. Consider ordering water or other
natural drinks in restaurants, and during social occasions. Take a break for
a couple days; if this is very difficult, you likely have a problem and help
may be necessary. Contact your doctor, counselor, or look up the AA
(Alcoholics Anonymous) chapter in your area. Know that it is usually
difficult to give up something; it?s better to add something healthful, like
walking and breathing. It also helps to talk to others who have recovered
from their addictions and see what they did with their extra energy; helping
others often helps you as well.


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In Sight - Italy for iPhone
$3.99 Hvard Iversen iOS Version 2.1 Full Specs
Publisher's Description
From Hvard Iversen:
What is the name of the mountain you can see in the distance? What are the lakes you see in front of you? Point there with In Sight Italy and you'll see on the screen what they are called."App of the Week" in Norway's largest newspaper Aftenposten on May 12th, 2012: "It's as simple as it is brilliant!" * Offline Augmented Reality in Italy * See what's near you where you travel in Italy: Mountains, Lakes, Places, Buildings, etc. You don't even have to be nearby Italy: You can manually set your location to a place in Italy to pretend you are there. Decide if you want to see places nearby, or famous cities around the world. Works everywhere: By the sea, in the mountains, in the city. Without the internet, without coverage. Turn the phone sideways to view on the camera screen the places that are nearby. Take a picture of what you see, and send it by email, or post it on facebook or twitter, directly from the App! You can also add your own places! In Sight Italy is also an altimeter, speedometer and also estimates the distance you've traveled. Choose between metric (km, meter) or imperial (miles, feet) units. All data is built into the App, so you don't need internet. You only need internet when you use the map or send a picture on email or post it on facebook and twitter.Select between the following map types: Standard, Satellite and Hybrid.In Sight Italy relies on the compass and GPS of your device. Best results are achieved if you are outside.The facebook integration requires iOS 6 or later. Select settings on the home screen, and look for the facebook icon.The twitter integration requires iOS 6 or later. Select settings on the home screen, and look for the Twitter icon.Note: If your device don't have a built-in compass, you cannot see the places as camera overlays (3rd generation iPod touch, iPhone 3G or older models). You can still see the places in lists and on the map. Source: ws.geonames.org under the Creative Commons Attribution 3.0 License (http://creativecommons.org/licenses/by/3.0/)


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K1S1 Extreme Knitting Yarn in Fawn 5kg bump
K1S1 Extreme Knitting Yarn in Fawn 5kg bump
Price is in Australian dollars (ex. GST).
GST will be added to Australian orders at checkout.
K1S1 is Little Dandelion's DIY extreme knitting yarn. Made in New Zealand by a specialist Mill to my specifications from fine grade merino wool, K1S1 replicates the same scale I create using unspun wool without requiring the same time consuming processes. K1S1 is not a rug yarn. It is a high grade luxurious fully felted technical knitting yarn specifically designed for textile use. Unlike unspun wool, K1S1 will not shed, has a high degree of functionality and a low pilling quotient.
K1S1 is sold in 5 kilogram bumps so that you can knit a throw without joins.
Before purchasing a bump, please read my Hot Tips and the Disclaimer on the K1S1 page. Knitting on such a large scale is not for the faint hearted so you need to be sure you are up for the physical challenge.
100% felted fine merino wool tops
Made in New Zealand
Throw Dimensions:
A bump this size will make a throw approximately 130cm x 170cm if knitted with a regular knitter's tension. My standard throw typically measures around 135cm x 185cm using a loose tension.
Approximately 5 kgs.
A 5kg bump contains approximately over 200m of yarn.
Before knitting, I recommend unwinding lengths of yarn at a time and allowing it to recover its shape before knitting. This process will naturally continue over time.
Infrequent Dry Clean Delicate recommended. A gentle hand wash in lukewarm water using wool wash is possible although the size of the made up throw makes this a big and wet project. If you are up for it, remove as much as the excess water as you can before drying. A gentle spin cycle in your washing machine is effective if you think the throw will fit. Lay flat to dry. Drying in the shade is recommended as exposure to direct sunlight may cause the wool to fade.
Handle and store with care. Due to the delicate nature of the wool any contact with rough surfaces and sharp objects may result in pulls and damage.
K1S1 has been specially designed to have a low pilling quotient. However, pilling may also occur as it is a natural result of friction. It can be easily removed by snipping off any "balls" of wool. Pilling does not constitute a defect.


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Child Proofing
Child Proofing
Infants and young children have insatiable curiosity, and a boundless sense of wonder. Unfortunately, this can serve as a source of tremendous stress for parents. You must constantly keep an eye on your little ones, in order to ensure that they aren't getting friendly with the electrical outlets or exploring the brightly colored liquids beneath the sink. At HomeSecurityStore.com, we know how daunting it can be to child-proof your home, and that's why we offer a wide array of effective, easy-to-install childproofing devices for every household. We believe that the simplest changes can make all the difference in the world during those pivotal moments when your child is most vulnerable.
Child Proofing 101
Childproofing isn't difficult; it just takes a bit of foresight. Take a close look at all of your cabinets and drawers, and consider whether each one contains potentially hazardous substances, including chemicals, medications, sharp objects and anything that a child can potentially choke on. We recommend installing cabinet locks on all cabinets, and we also recommend installing door latches to prevent your kids from wandering off into dangerous areas inside—or outside—the house. Finally, if you own a swimming pool, you'll want to invest in a reliable pool alarm. You'll find it all here at HomeSecurityStore.com.
Save Money; Improve Your Peace of Mind
In recent years, childproofing has become an enterprise unto itself. Some parents are now opting to hire a professional "child proofer" to assess and modify their homes, but this is costly and unnecessary. You can easily childproof your own home with a bit of common sense and planning, and all it takes is a bit of equipment and a few household tools. At HomeSecurityStore.com, we make the process easy and effective, even if you're short on time and don't consider yourself to be much of a DIY-er.
Order Now
Browse our wide selection of child proofing essentials, including pool alarms, door latches, cabinet locks, windows bars, Velcro straps and more. Stock up on the tools you need to protect your precious little ones and renew your peace of mind. HomeSecurityStore.com offers the best deals, the highest-quality merchandise, and top notch customer service. Most orders ship same business day, and we are committed to your satisfaction. Order now.
Minimal Price: $19.99


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These days, wearing a giant robot on your shirt just isn't enough to be ostracized from the party. Step up your game with the the Swarovski crystal hand-stitched Gundam tee. Not only is it a worthy homage to your favorite battle robot, but to Swarovski-covered tech everywhere. (On a side note, when will manufacturers tire of the same old, cover it in crystal method of making gadgets more expensive. How about just manufacturing more amazing tech?)
So just how much is too much to spend on geek fashion? I'd wager $1,690 is, at minimum, a starting point for the discussion. [plasticbamboo]


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Organic Produce Guide
Martha talks about how to weigh your produce choices at the grocery store.
These days, picking out a head of lettuce can make you feel as though you need advanced degrees in agriculture, chemistry, economics, and nutrition.
Among the questions you may have: What am I getting when I pay for organic, and is it worth it? Do seasonal and local foods taste better, and are they better for me? When is frozen or canned produce a good alternative? And how can I balance these issues with the needs of my family?
That's a lot to consider when you're just trying to get dinner on the table. To help you thread your way through the thicket, here's the most up-to-date information on the most important issues. Your answers will depend on where you live, your values, your budget, what's available in your local markets, and, of course, what you like to eat.
What Is Organic?
No synthetic fertilizers or pesticides. That's the main thing to know about how organic produce is grown. Organic farmers instead use compost to enrich the soil and beneficial insects to kill plant-destroying pests. To use the word organic on their labels, growers must be certified under federal rules.
Once easiest to find at farmers' markets, organic fruits and vegetables are now standard fare alongside conventional foods in supermarkets and big-box stores. But farmers' markets and community-supported agriculture programs (CSAs -- produce subscriptions bought directly from farms) remain the purist's choice. Their goods come from small farms that stick most closely to the original organic ideal.
Since organic regulations took effect in 2002, gigantic organic farms have emerged and sometimes drawn criticism for using some of the methods of mass-scale agriculture, such as growing immense fields of just one crop for national distribution. But they also have slashed organic-food prices and brought organic produce to neighborhood supermarkets. Both big and small organic farms mean fewer chemicals in the environment.
Pesticide-free has no legal definition, but it may signify an operation that's in the three-year transition to organic status, or one that functions organically but chooses not to be certified. (Because organic certification is costly, some farmers don't bother, even though they practice organic methods.) Sustainable is a broad term that means farming in ways that build the soil (instead of depleting it) and don't cause pollution. Shopping at farmers' markets lets you ask the farmers directly how they grow their crops.
How Organic Stacks Up
Organic farmers like to say their strawberries or carrots taste sweeter (the food editors of Martha Stewart Living tend to agree). But there are multiple variables that can affect taste, including plant variety, sun, soil, farming practices, and ripeness. Small-scale organic farmers do often plant tastier varieties -- the kinds that won't stand up to shipping but are perfect for local markets -- and pick their fruits and vegetables when they're riper (that is, sweeter).
Food Safety
Pesticide residue is part of the equation, especially if you're feeding young children, whose systems are less developed and may be more sensitive to toxins than adults'. The EWG, a nonprofit environmental research organization, calls the 12 fruits and vegetables that carry the most pesticide residue "the dirty dozen." These include (in order of most residue to least): peaches, apples, bell peppers, celery, nectarines, strawberries, cherries, kale, lettuce, imported grapes, carrots, and pears.
The EWG's "clean 15," the fruits and vegetables with the least pesticide residue, are (in order of least residue to most): onions, avocados, sweet corn, pineapples, mangoes, asparagus, sweet peas, kiwifruits, cabbages, eggplants, papayas, watermelons, broccoli, tomatoes, and sweet potatoes. Grapefruit is number 16. Oranges and tangerines, staples of midwinter, fall midway on the list of the 47 fruits and vegetables tested.
The U.S. Department of Agriculture (USDA) says organic is cleaner but takes care to point out that "measured residues on most products, both organic and nonorganic, do not exceed government-defined thresholds for safe consumption."
When it comes to foodborne illness, organic isn't immune, as the 2006 outbreak of E. coli in organic bagged spinach proved. Contamination is all about who or what touches the produce along the way. Distribution of mass-produced foods will spread a tainted batch far and wide, while small farms have a narrower reach. Organic food can't be irradiated, a controversial technique approved to kill pathogens in some foods, or genetically modified, a practice whose long-term safety is unknown.
Organic is still more expensive than conventional produce, for the most part because many of the farms are small and the farming methods are labor-intensive. Wholesale prices tracked by the Rodale Institute, a nonprofit organic-research group, vary widely from market to market. Organic acorn squash, for example, costs 20 percent more than conventional in Seattle but about 200 percent more in Boston.
If you have limited dollars to spend on organics, devote them to organic varieties of the EWG's "dirty dozen." You'll get the most pesticide-free bang for your buck. You can also save by buying conventional varieties of "the clean 15." (The lists are available at foodnews.org/walletguide.php and can be downloaded as an iPhone application.)
Hands down, organic is the winner here: By definition, buying organic means fewer chemicals on the land and in the water and air. Runoff from chemical fertilizers used on Midwestern corn and soy fields, for example, has created a 3,000- square-mile "dead zone" in the Gulf of Mexico, where algae grab all oxygen out of the water, starving any other life.
Whether organic produce contains more nutrients than conventional is a matter of dispute, with many scientific studies backing both sides. The jury is still out.
Bottom Line
Buy organic whenever you can; it's best for the environment. Exceptions: if it's flown in from across the world and you have a beautiful, local -- but not organic -- carrot right under your nose, or if it's mass-produced and you have the option to buy from a reliable small farmer instead.
What Is Local?
Locavores, people who seek out locally grown foods, want to cut energy use by reducing the distance their broccoli or lemon wedges travel to their plates. The rule of thumb is that most produce is transported 1,500 miles to market; local foods travel fewer than 100 miles. Those figures come from a study of Midwestern markets, and numbers depend on where you live and the season. Locavores also want to support farmers in their area. (Author Barbara Kingsolver wrote about her experiment with growing her own food and eating locally in the 2007 book "Animal, Vegetable, Miracle.")
The concept works best for people who live in places with warm climates and lots of farms, such as California. For residents of most other states, going local takes more effort. In winter, root vegetables, some hothouse-grown foods, and canned and frozen produce have to suffice. Banana lovers would be pretty much out of luck in any state but Hawaii.
How Local Stacks Up
Local has a leg up because fruits and vegetables that won't be shipped far can be picked riper -- and the riper they are, the better they taste. This is especially true of sweet summer crops, including tomatoes, peaches, and nectarines, whose sugars develop late and fade fast. (Citrus, apples, avocados, and beets and other root vegetables store and travel better.) In addition, fruit and vegetable varieties grown for shipping are usually bred for strength, not flavor.
Food and Safety
Eating food from farmers you know lets you ask them what chemicals and growing methods they do or don't use. Ideally, the farmers will be less likely to cut corners to drive down prices if they have a relationship with their consumers.
A mixed bag that depends entirely on what's grown near you and what season it is. Theoretically, cutting transportation costs would mean lower prices. But farmers who sell small amounts at farmers' markets have higher costs per pound than giant farms that send boxed and bagged spring mix or carrots all over the country. So you won't always find bargains. The money you spend on local foods, though, helps the surrounding community.
Buying locally means fewer "food miles," the measure used to count how far your dinner traveled to get to your table. This can mean less energy used and less pollution. It's worth noting that food miles don't take into account the energy used to produce and harvest food (by operating farming machinery, for example), which can equal or exceed the energy needed to transport it. So miles don't always tell the whole story.
Also on the plus side: Supporting local farmers makes small farms more economically viable, creates carbon-absorbing greenbelts, and helps teach children where their food comes from.
Local foods are consumed closer to the time they're harvested, before nutrients dwindle. They also spend less time in transit; long periods of travel can sap produce of nutrients.
Bottom Line
Support small farms near your home. That can mean buying conventional produce locally over organic from far away. Get to know the people growing your food. Create a relationship of mutual trust. Your purchases speak volumes at the register. Localharvest.org provides listings of local markets, CSA programs, and more, searchable by ZIP code.
What Is Seasonal?
Ideally, we'd always buy fresh fruits and vegetables at the height of their growing season because they're better and cheaper. Think about the pricey, pinkish tomatoes in stores right now, compared with the red, juicy bargains of late August.
But it's not always easy, especially in winter. One approach is to make menus seasonal whenever possible and then augment them with preserved goods. High-quality frozen, canned, and jarred produce is increasingly available in stores, and home canning is making a comeback. In addition, more local growers are preserving and freezing peak produce and selling it at winter farmers' markets. Many vegetables and fruits -- green beans, corn, peas, edamame, berries, and mangoes, to name just a few -- freeze well. Tomatoes, peaches, nectarines, and pears are canning stars.
But even in winter, there are many seasonal delights to be had: oranges, grapefruits, pomegranates, winter squashes, spinach, cabbages, broccoli, carrots, potatoes, kale, parsnips, beets, Swiss chard, and more.
How Seasonal Stacks Up
In-season, fresh produce tastes best -- if it's grown well and consumed soon after picking. But out of season, the argument can be made that fruits and vegetables frozen at the height of their season are more flavorful than those shipped from far away. Freezing maintains flavor better than canning, but it can damage texture. Frozen berries make good smoothies, however, and frozen vegetables work well in stews, braises, and other hearty winter fare.
Food Safety
One consideration is where produce is grown. In general, U.S. growers use lots of pesticides, but tests show that imported produce has more residue. Imported produce tends to fill seasonal gaps here (grapes, for example, which are high on the residue list). Check packages of frozen fruits and vegetables for their country of origin. Lots of processed food is imported. China, which increasingly exports fresh and processed fruits and vegetables, including organic ones, has had major problems with food contamination and lax production practices in the past few years.
One interesting benefit for frozen and canned goods: According to Organic Center, an advocacy group supported by organic-food companies, freezing or canning can actually reduce pesticide residue in some fruits and vegetables, via washing, peeling, or cooking. Apple juice and applesauce, for example, contain less residue than fresh apples. Processed green beans have only a tiny proportion of the pesticides found in fresh, and canned peas and tomatoes are cleaner than fresh. But processed strawberries show little difference. Presumably, thorough washing and peeling of fresh produce would achieve the same results at home, though the study doesn't say.
Come January and February, farmers almost can't give away all the beautiful kale; in summer, it's tomatoes and zucchini. In general, fresh fruit and vegetables cost less than frozen or canned, according to USDA analyses. But that varies by season and region, so it's best to check for yourself.
Buying South American asparagus or grapes in winter requires more fossil fuels, which can generate more pollution, than buying foods grown closer to home. Out-of-season hothouse and hydroponic foods, such as tomatoes and lettuce, may also require significant amounts of energy if growers are not using energy-efficient methods.
Seasonal means better nutrition because many out-of-season vegetables and fruits -- say, winter peaches from South America or avocados from Mexico -- have to travel long distances and lose nutrients along the way.
In deep winter, frozen and canned vegetables are good options; they tend to be picked fully ripe at the height of the season, their nutritional peak. Canning without cooking can preserve most nutrients. Watch out for salt in canned vegetables, however, and sugar added to canned fruit and some frozen berries.
Bottom Line
We've become spoiled by out-of-season produce, but it's time to get back to basics. Buying with the season saves money, helps you stay local, and makes for the best-tasting food. Canning and freezing can help. Think of how incredible that first fresh strawberry will taste if you've had to wait all winter for it.
And What About. . .?
If you're wondering what fair trade means when it comes to your bananas, whether GMO foods are a cause for concern, or what it takes to grow hothouse tomatoes, here are the facts behind these and other food trends.
Genetically modified organisms, also called genetically engineered (GE) or biotech foods, are bred using techniques that allow scientists to introduce genes from other plant varieties, animals, bacteria, or viruses into a plant to give it certain traits. The technology is used widely to create crops resistant to pests or able to withstand chemical bug and weed killers.
Corn, soy, and canola are some of the largest GMO crops, but other vegetables and fruits aren't far behind. According to the True Food Network, a project of the anti-GMO Center for Food Safety, biotech versions of 32 common fruits and vegetables, including grapefruits, apples, carrots, and cabbages, are being field-tested. Corn, papayas, radicchio, and yellow crookneck squashes are already on the market.
Advocates say biotechnology is the way to create superfoods to feed the world. Critics are concerned about the potential for environmental effects (because GMO traits can spread widely) and health problems down the line (because the foods are so new, their long-term effects on humans aren't known).
How can you tell if you're buying a fresh GMO fruit or vegetable? If the PLU (price look-up) code on the sticker has five digits, and the first one is eight, it is. (Four-digit numbers starting with eight are not.) The labeling system, however, is voluntary.
Using radiation (gamma rays, X-rays, or an electron beam) to kill diseasecausing bacteria and other microbes has been practiced for years on many foods, including herbs, spices, meat, and produce. Government and industry groups see the method as a way to improve food safety, because it destroys pathogens such as salmonella and E. coli. But consumers have resisted, wary of any technique that uses radiation.
Irradiation doesn't make food radioactive. But depending on the dose, it can change a food's texture and taste. Organic foods cannot be irradiated, and many organicfood advocates prefer other methods of getting pathogens out of the environment, such as keeping cattle-feedlot manure out of streams.
Irradiated food must be labeled with a flowerlike symbol that reads "treated with irradiation," but the food industry would like to remove any labeling requirement.
Fair Trade
Mainly seen on bananas and coffee, this label indicates that the farmers who grew the food received a fair price for it. Fair-trade crops often cost more, but not always. There is no clear effect on flavor, nutrition, and safety.
Hothouse & Hydroponic
Growing produce in hothouses makes it possible to buy fresh even in winter, with varying effects on flavor. Hydroponic produce -- often tomatoes and greens -- are raised indoors, under lights, in nutrient-packed water, or in a growing medium instead of in soil. Yields are high, but energy and water use can be, too. The good news is that growers are turning more and more to "green" options, including solar and geothermal technology, to make these methods more energy-efficient.
We've all seen it: chopped fresh produce, including celery sticks, broccoli florets, and melon balls, sold neatly wrapped in plastic and ready to go. But it is not a good buy. Flavor is compromised in the processing (the produce is usually treated to keep it from browning or spoiling), precut produce is expensive, and the plastic packaging is wasteful and can clog landfills. Plus, more processing means your food is handled by more people before it reaches your plate.


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The big Samsung vs. Apple trial is only part of the patent infringement war between the two companies. Bloomberg has reported on the progress of another case involving the two leading smartphone manufacturers, which concerns an Apple patent on unified search. Apple wants to secure a ban on the Galaxy Nexus being sold in the United States. Samsung, naturally, disagrees, arguing that it shouldn't be banned. The trial isn't scheduled to start until March 2014 so the importance of deciding on a ban now is clear.
Samsung has already started removing the unified search feature from some of its products, including the Galaxy S3, although it's far from clear whether Apple can really prove the validity of this patent. How can Apple lay claim to the idea that you can search multiple sources, such as the Internet and contacts simultaneously, on a phone? Even if it can, should a smartphone be banned from sale because of one small feature?
Apple argues loss of sales
Apple's lawyer Perry spoke against the Nexus, contending that it was Samsung's big attempt to take down the iPhone. "This was the top of the line, Cadillac phone they trotted out to compete with the iPhone."
He went on to suggest that anyone choosing the Galaxy Nexus over the iPhone could be lost to Apple as a customer because people tend to stick with the same operating system on their next phone. He also spoke about the loss of Apple revenue for things such as applications and accessories.
Samsung argues "minuscule" sales
Samsung lawyer, John Quinn, argues that the idea that the Galaxy Nexus has put a dent in iPhone sales is ridiculous. He said, "This is a product that, at most, captured 0.5 percent of the market."
With sales of $250 million from its first two quarters on the market, the Galaxy Nexus is hardly an iPhone beater. That compares to sales of $16.2 billion for the iPhone over the same period.
Google reportedly weighed in by saying the patent relates, "at best, to a very minor aspect of the Galaxy Nexus phone's overall functionality which was never shown to drive sales."
Sprint also got in on the act, calling itself an "unwitting victim" and pointing out that the ban would harm its reputation.
Central argument is hard to swallow
Apple is essentially saying that Samsung copied the unified search feature, which is apparently what makes Siri so compelling. Can it prove that it lost market share to the Galaxy Nexus, or that the unified search feature stimulated sales of the iPhone or the Galaxy Nexus? It seems unlikely, but then again, it seems unlikely that a company could be granted a patent for this in the first place.
As Google went on to point out, smartphones are packed with features, and if patent owners block sales of devices based on single features then "the design and manufacture of similarly complex technological marvels would become economically infeasible."
But Google may not be listening to its own advice. Google's Motorola filed a new patent lawsuit against Apple to block imports of the iPhone, iPad, and Mac computers into the U.S. — a signal that the gloves are coming off. It looks like Google vs. Apple is kicking off; even before the Samsung trial is settled.
There's no end to this patent madness in sight and, as we discussed before, it's consumers who will end up paying for it, one way or another.


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1) Review the Upgrading Guide. It will have some general information about upgrading our products that is useful.
2) Additional articles you might want to review are the Install Guide and the Visual Studio Binding article.
3) Back up your sgvault, sgmaster, and sgdragnet databases.
4) Uninstall the Fortress server, but choose the option to keep the databases.
5) Install Vault Professional 5.1.2 server. Then go in steps through the major versions as instructed in the Upgrading Guide.
6) Users will uninstall the Fortress client and then install the Vault Professional client.
7) If using integration with other software such as Visual Studio, follow the rebinding instructions found in this article: Binding and Rebinding.
8_) Make sure you make a new database backup of the Vault databases after the upgrade is completed. Depending on what version you are going to, you might have additional databases to backup. For example, in Vault 7.x, you will backup the sgvault, sgmaster, sgdragnet, sgvaultindex, and sgnotify databases.
Moving from Fortress to Vault Professional
A collection of information about Vault Professional, including solutions to common problems.
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Story highlights
- Security agency says 20 servicemen, 7 crew members were on the AN-72 aircraft
- The acting border patrol chief was aboard
- A Russian news outlet said it crashed in southern Kazakhstan
A Kazakhstan border patrol plane with 27 people on board has crashed, the country's National Security Committee said Tuesday.
Twenty servicemen and seven crew members were on the AN-72 plane when it crashed in southern Kazakhstan, in central Asia, the committee said in a statement.
Turganbek Stambekov, the temporary director of Kazakhstan's border patrol service, was among those on board, the committee said.
The statement was reported by Russian and Kazakh news outlets.
The head of the regional emergency services department, Valikhan Derezhepov, said the plane went off radar screens about 5 p.m. Moscow time and a resident called rescuers within minutes to say he saw a plane crashing, Russian state-run RIA Novosti reported.
First responders raced to the scene, near the city of Chimkent. Kazakh authorities are investigating.


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|2012-07-25, 03:13
|Link #1
Senior Member
Join Date: Dec 2005
One Piece - Chapter 675 [manga]
Welcome to the weekly manga discussion thread for One Piece.
Thread Guidelines
Well, the break's over (for now), AND we've got Aohige and T bringing out spoilers this week! Let's see what happens next:
-Chapter title is "The name is Shinokuni". As for the new ministory, it does indeed seem to center around Caribou, as this chapter involves him terrorizing innocent mermaids once again.
-Caesar's slave children are begging for candy, and he tells them that it's in the Biscuit Room. He then has his men lock the door, just as one of the kids regains their senses, remembers Nami and Usopp's warnings, and is now willing to escape. Meanwhile, Nami has regained consciousness due to being in Sanji's stronger body, and wakes up Usopp.
-It's revealed that Law made a deal with Caesar that involves the shichibukai trading hearts with Monet, though the former's heart is currently in Vergo's hands. It also turns out that Monet shapeshifted and was stalking Law under his nose, which is why CC is aware of his treachery. It seems the scientist returns to the lab and punches Law's heart (unsure if this means Vergo's still around, though). Smoker's heart is in the scientist's hands, as well. Monet then says that she prepared the video cameras for the new experiment.....
-Criminals from all over the world are watching the video footage, which first shows a huge piece of candy lying in the middle of the icy half of the island. We then shift to the hot side of the island, where Smiley (the slime) is still reforming himself. Caesar explains that the slime itself is the poison gas bomb "H2S". Apparently, the previous incarnation of the bomb could weaken people, but didn't kill them instantly. However, all the new slime has to do now is eat giant food and it will become a deadly weapon of mass destruction. And thus, Caesar has decided to dub the weapon "Shinokuni" (or Land of Death). During the speech, Zoro's group, who are still running from the slime, come across the giant candy and some of Caesar's men. The servants are meant to be sacrifices for CC's experiment, though he pretends to look out for their well-being. However, as soon as Smiley appears, he just eats the giant candy and seems to be ignoring his master's orders.....
Heh, so we've already got signs that the poison salamander is going to betray CC. Though I do fine it interesting to learn that Law and Monet traded hearts. I wonder where Happy Harpy's heart is being contained, in that case? And I'm also still unsure if Vergo is in the same room as CC when he returns to the lab, or he went to hide. Guess I'll wait until the full chapter's release before I make any corrections on that part.....
I also wonder how Caribou's story will go down. Apparently, he's kidnapping mermaids again, but I highly doubt that guys like jinbei are going to sit idly by and let him do as he pleases. So maybe this ministory will involve him escaping the island so he can deliver the info of Shirahoshi's secret to that info broker he was talking about a while back.....
Last edited by marvelB; 2012-07-25 at 09:20.
|2012-07-25, 06:46
|Link #2
lost ronin
Join Date: Oct 2008
Location: in the recesses of my convoluted mind...
so i guess now we know why law suddenly fell "ill" when he saw vergo, the guy had his heart! but why exchange hearts with monet when he could have exchanged hearts with ceasar himself? he was that desperate that he was willing to risk it?
|2012-07-25, 07:22
|Link #4
Kana Hanazawa ♥
Join Date: Jun 2007
Location: France
Age: 32
I don't get why Law traded his heart for Monet's either. It's not like Ceaser cares about his subordinates, so having Monet's heart doesn't give Law any kind of leverage against him. He totally screwed himself. He must have been really desperate to get his hands on... whatever it is he wants.
Monet has a shape-shifting power? Kind of confused about that line.
|2012-07-25, 08:22
|Link #5
Senior Guest
Join Date: Jan 2009
Location: Athens (GMT+2)
Age: 30
From what I gather, Law was hoping to use the possession of Monet's heart to exert a degree of influence on her without forcibly making her his ally. Too bad she didn't care as much. The odd part is that Law was definitely aware of her ability, whatever it is, when he asked her to follow him, so how did she stalk him without being spotted? Heartless Law is in quite a pickle, he can do nothing until Vergo is taken care of - which will be pretty hard, considering he holds Law hostage in any case.
So, the chapter goes from real serious, what with the giant kids, imprisonment and massive B.O.W.s to cookie jokes (which was curiously funny) and smiley rampage? Well, that works...so far
|2012-07-25, 08:49
|Link #6
Senior Member
Join Date: Dec 2005
Interesting that you mention this, because Aohige mentions in the spoiler thread that CC implies that Law is actually STRONGER than him (though I think the current chapter translation implies the opposite). So the heart exchange thing pretty much IS insurance in case Law betrayed him (which he most certainly did).....
Anyways, fun chapter. I'm loving those new goofy expressions from CC. In fact, he's pretty much becoming the most expressive OP villain since Moria. And is it just me, or does one of the NW pirates shown this chapter sorta look like Basil Hawkins with a cowboy hat.....?
And yeah, looks like Vergo's in cahoots with CC after all. The fact that he's in possession of Law's heart instantly explains how he made Law collapse when he first appeared. Though I'm now wondering where exactly Law is keeping Monet's heart (BTW, I found it rather odd how easily she agreed to give up such a vital organ in the first place)....
|2012-07-25, 11:56
|Link #7
Senior Member
Join Date: Dec 2007
Law being critically weakened when confronting Vergo makes a lot of sense now. Whatever it is that Law wants, it must be absolutely imperative that he gets it if he had to sacrifice his heart. And if CC has to resort to stealing Law's heart as an "ace in the hole" in case anything goes wrong, it means that under normal circumstances Law is very much a big threat to him (and presumably, Vergo as well).
Interesting development in this chapter.
|2012-07-25, 14:10
|Link #9
Senior Member
Join Date: Nov 2011
not really a big fan of the heart stealings... i thought it made much more sense for vergo to have some sort of gravity ability which would be a good counter to law's ability. it also would have explained the food on his face to a degree. this development actually undermines vergo's coolness, since he didnt really do anything himself to pin down law, he was just carrying around his heart... i dunno. doesn't really do it for me
|2012-07-25, 14:17
|Link #11
Senior Member
Join Date: Dec 2005
I think a bird (even if it's small) would be a little too conspicuous in a location like Punk Hazard. My guess is that she can transform into something else entirely separate from a bird. I mean heck, Monet could even be an amoeba zoan for all we know!
BTW, kinda unrelated, but the cover for volume 67 has been revealed:
Nice! So we've got Kinemon, Monet, AND CC in color (at least in his gas form as far as the last one is concerned). But there's a couple things I find odd here: First, Law isn't on the cover for some reason (unless Oda's saving an epic pic of him for the NEXT volume's cover), and.... do I see human legs on Monet's harpy form?! I find that odd since the past chapters showed her legs were talons. Either this is a mistake on Oda's part, or we just got another hint that the harpy body is indeed her zoan form (meaning I'd have to disregard my above joke about her being an amoeba woman)......
Also, I suppose some of you may be wondering about that One Piece White book I mentioned a few threads back. Well..... it turns out that the book focuses on the anime version, so it doesn't look like we can expect any new manga info, unfortunately. But on the flipside, volume 67 is released next week, which of course means the possibility of some sweet SBS info.
Dunno about you, but I think the mere fact that CC entrusted the heart to Vergo in the first place has a lot of significance. And besides, it wouldn't make sense for him to have a gravity-based ability when that power was already taken by Blackbeard, don't you think?
|2012-07-25, 15:00
|Link #12
Senior Member
Join Date: Jul 2008
Location: 1431 Highland Drive
The main highlight for me is that the slime is mentioned as the weapon of destruction. Does the H2S mean 2 parts Hydrogen 1 part Sulfur?
|2012-07-25, 15:50
|Link #13
Join Date: May 2010
Location: Unspecified
it was kinda disappointing chapter. i blame partly because of there is breaks before and after this chapter.
|2012-07-25, 15:50
|Link #14
Senior Member
Join Date: Dec 2007
|2012-07-25, 15:51
|Link #15
Senior Member
Join Date: Dec 2005
Also about Monet if you look at a manga pic of her
Spoiler for image:
You can see that her talons actually start below the midpoint of her shin, to close to where her human ankles might be; so the volume pic is actually cutting her off right before the talons... granted looking at that manga pic in this context i can't help but think that her human thighs are actually a little long; before i did not mind since i assumed her legs were ALL bird and not just the bottom portion
|2012-07-25, 16:42
|Link #16
ANEGO Worshiper
Join Date: Jun 2011
Location: By the vending machine, drinking tea.
An Oda drawn image that proves Nami is in love with Luffy.
Any fact that counters this will easily be disregarded by the LuMi fans.
|2012-07-26, 02:07
|Link #18
Senior Member
Join Date: Nov 2011
|2012-07-26, 09:35
|Link #19
Senior Member
Join Date: Dec 2005
The whole "altering weight" power was already taken by Ms. Valentine, though. And technically, the power you had in mind for Vergo should be feasible in Teach's case as well, since he now has both gravity AND earthquake powers. I'm also unsure about telekinesis like BDK mentioned before, since I think that sort of strikes me as something that could be achieved without the aid of a devil fruit (after all, excluding haki users, we've actually seen non-DF users who had powerful hypnotic abilities like Ms. Goldenweek (and even Jango to some extent)).
But still, just because he didn't weaken Law with his own abilities doesn't mean that Vergo isn't dangerous in his own right, as was already mentioned. The dude's a VA-level marine who ALSO doubles as a close confident of Doflamingo's. It would be flat-out insane to think that he'd be a pushover at this point.....
You know, now that you brought this up, this makes me curious: I wonder how many LawxMonet doujins are going to pop up now due to the combined effect of this heart-trading business and their brief interaction before Vergo's arrival?
Lastly.... this is a bit random, but seeing as Smiley gets stronger by consuming candy, I think it would've be the most awesome thing ever if Smiley got his ultimate power-up when CC feeds him some super-sized Chick-O-Sticks. Again, I know this sounds random, but I was snacking on some last night when this thought suddenly popped in my head, and I just couldn't help but voice it!
|2012-07-26, 15:10
|Link #20
Annie Leonhart
Join Date: Jul 2007
Location: Indiana
Chapter 675
Okay chapter. Now there is Luffy's crew and the alliance, Smoker and G-5 Marines, Blob, Ceaser and Vergo team up. It is a four way death match by the looks of it. Ceaser is turning out to be a huge coward. He sounds like the weakest out of Luffy, Law, Smoker, and Vergo.
|Thread Tools


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From last Saturday's game between the Dodgers and Mets in Citi Field. 8th inning and the Dodgers do a pitching change.
Matt Kemp needs to relax since he knows the bullpen might blow this up.
Video -
Monday, May 9, 2011
Matt Kemp Dancing @ Citi Field
Posted by Roberto Baly at 1:30 PM
2 comments:
- nymets_fan said...
It was right after this that Kemp misplayed a ball hit by Justin Turner into a game winning double.
- May 11, 2011 at 1:52 PM
- vinnie said...
On the bright side, if the Dodgers don't make payroll, he can get a side job as on Dancing With The Stars
- May 11, 2011 at 6:23 PM
Subscribe to: Post Comments (Atom)


text:
Lawmakers have made some valuable changes to the law covering public charter schools but have punted on the most important issue.
Charters are independently operated publicly funded schools. Gov. Tom Corbett and legislative Republicans have pushed hard for the expansion of charter schools, believing that they will improve education and lower costs by making conventional schools compete for students.
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Two new wallpapers
Posted Aug 3, 2009. Leave a comment?
Finally, the wallpapers for July and August 2009 (the first a bit late) are up!
As always you find the wallpaper with the calender for the individual month and without the text in the sizes 1280×1024, 1024×768 and 1280×800!
Later on I have to check the music video section—as it seems YouTube had removed a lot of videos *sighs*


text:
In this issue
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|Developing sensitive chemiluminescence ELISAs—DynaLight™ Substrate with RapidGlow™ Enhancer
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|Accurate microRNA quantitation—MyQubit miRNA Assay
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|Enzyme-mediated methodology for the site-specific radiolabeling of antibodies based on catalyst-free click chemistry
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Find product information, science news articles, special offers, and more
On the web
Model systems for Parkinson's disease, and
flow cytometric assays for apoptosis
Imaging corner
Reducing endogenous background with the Endogenous Biotin-Blocking Kit
Highlight from BioProbes® Journal
Live-cell staining of pluripotent stem cells: Alkaline Phosphatase Live Stain for early identification of induced pluripotent stem cells
Molecular Probes® webinar series
Fluorophore selection, and
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6th Annual Great Plains Analytical Cytometry Association (GPACA)
September 13
University of Iowa, Iowa City, Iowa, USA
Great Lakes International Imaging and Flow Cytometry Association (GLIIFCA) 2013
September 27–29
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Ohio River Valley Cytometry Association Annual Meeting
September 18
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BioProbes® Journal of Cell Biology Applications
The Molecular Probes® Handbook
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ABfinity™ antibodies are produced by transfecting mammalian cells with high-level expression vectors containing immunogen-specific heavy- and light-chain rabbit antibody cDNA. This highly reproducible process results in superb consistency in lot-to-lot antibody performance.
ABfinity™ oligoclonal antibodies are mixtures of recombinant monoclonal antibodies. These combine the improved signal strength that can come from using polyclonal antibodies, with the highly reproducible results you get from ABfinity™ monoclonal antibodies.
Immunohistochemistry analysis of human uterine leiomyoma tissue section probed with ABfinity™ smooth muscle actin recombinant rabbit oligoclonal antibody. Horseradish peroxidase (HRP)-conjugated goat anti–rabbit IgG was used as secondary antibody. Diaminobenzidine (DAB) was used to reveal the HRP, and hematoxylin was used to stain the nucleus.
|Cat. No.
|Smooth Muscle Actin Oligoclonal Anitbody, Rabbit Recombinant
|100 µg
|Smooth Muscle Actin Monoclonal Anitbody, Rabbit Recombinant
|100 µg
MyQubit miRNA Assay
What it is
The MyQubit miRNA Assay for use with the Qubit® 2.0 Fluorometer allows easy and accurate quantitation of small amounts of miRNA even in the presence of ribosomal RNA, using a combination of existing Life Technologies reagents, common buffers, and the MyQubit miRNA Assay file. The MyQubit miRNA Assay is based on the Quant-iT™ OliGreen® ssDNA Reagent, which exhibits a large increase in fluorescence upon binding to nucleic acids.
What it offers
- Sensitivity—accurately detect as little as 0.5 ng miRNA, even in the presence of ribosomal RNA
- Simplicity—add your sample (in any volume between 1 μL and 20 μL), then read the concentration using the Qubit® 2.0 Fluorometer.
How it works
The MyQubit miRNA Assay is selective for small RNA, unlike other RNA detection methods such as the NanoDrop® UV-VIS spectrophotometer A260 assay or even the Qubit® RNA assays (see figure). The MyQubit miRNA Assay has a dynamic range of 5 to 500 ng/mL miRNA. The MyQubit miRNA Assay file can be downloaded from the bottom of the Qubit® 2.0 Fluorometer web page and permanently uploaded to your Qubit® 2.0 Fluorometer. In addition to the MyQubit miRNA Assay, the MyQubit firmware preloaded on all new Qubit® 2.0 instruments allows you to create other assays for your Qubit® 2.0 Fluorometer. Because the instrument is operated by simple commands, creating additional applications can be as straightforward as matching the spectral characteristics of the assay with the right LEDs and emission filters.
- Get instructions for downloading and installing the .qbt assay file from the MyQubit microRNA Assay manual (pdf)
- Download the MyQubit miRNA Assay .qbt file
Comparison of detection techniques for accurate quantitation of small RNA in the presence of ribosomal RNA. rRNA at the concentrations listed was spiked into solutions containing 2 ng/µL siRNA, then read using the MyQubit miRNA assay, the Qubit® RNA assay, or by 260 nm absorbance (A260) on the NanoDrop® spectrophotometer.
|Cat. No.
|Qubit® 2.0 Fluorometer
|1 instrument
|Quant-iT™ OliGreen® ssDNA Reagent
|1 mL
|Silencer® Select GAPDH Positive Control siRNA (Hs, Mm, Rn)
|20X TE Buffer, RNase-free
|100 mL
|5 g
|Qubit® Assay Tubes
|1 set
New anti–human CD33 conjugates for flow cytometry
What it is
The Molecular Probes® portfolio of over 1,000 highly specific primary antibodies for flow cytometry is expanding to include more Research Use Only (RUO) selections. The anti–human CD33 monoclonal antibody (mouse-derived clone WM53) has been conjugated to a variety of fluorophores to expand your research options.
What it offers
- Trusted—Molecular Probes® brand
- Validated—all antibodies are tested in flow cytometry applications
- Selection—expanded offerings of primary antibody conjugates for flow cytometry
How it works
CD33 is a type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after the appearance of CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent from granulocytes.
|Cat. No.
|CD33 Mouse Anti-Human mAb (clone WM53) PerCP-Cy®5.5 Conjugate
|0.1 mg
|CD33 Mouse Anti-Human mAb (clone WM53) APC Conjugate
|100 tests
|CD33 Mouse Anti-Human mAb (clone WM53) Fluorescein (FITC) Conjugate
|100 tests
|CD33 Mouse Anti-Human mAb (clone WM53) PE Conjugate
|100 tests
|CD33 Mouse Anti-Human mAb (clone WM53) PerCP Conjugate
|100 tests
Enzyme-mediated methodology for the site-specific radiolabeling of antibodies based on catalyst-free click chemistry
Zeglis BM, Davis CB, Aggeler R et al. (2013) Bioconjug Chem 24(6):1057–1067.
Antibodies are selective enough in their binding to offer the promise of an effective drug- and radioisotope-delivery system targeted at tumors. Researchers have long tried to exploit antibodies to do just that, and some have been successful. However, the lack of site selectivity when radiolabeling antibodies by most methods can result in inactivation of the antigen-binding domains, and this has slowed progress in the development of these potentially powerful tools.
Zeglis et al. have recently published a method for using catalyst-free click chemistry to radiolabel antibodies exclusively in the heavy-chain Fc domain, well separated from the antigen-binding domains. The method relies on the fact that IgG antibodies have a conserved N-linked glycosylation site on the CH2 domains of the heavy-chain Fc regions. The biantennary glycans that decorate those glycosylation sites are amenable to modification, and the method described is a stepwise enzymatic modification of the glycans, followed by a click chemistry process that results in radiolabels linked only to the glycosylation sites, well away from the antigen-binding sites.
We have taken this site-specific antibody labeling strategy and made it available in our SiteClick™ Antibody Labeling Kits. The SiteClick™ Antibody Labeling Kits make it easy for you to specifically label the Fc regions of IgG antibodies with R-phycoerythrin, any of an assortment of our most popular Alexa Fluor® dyes, or bright and photostable Qdot® nanocrystals.
- Watch a video about easy and site-specific labeling of an antibody using click chemistry
|Figure 1. The SiteClick™ antibody labeling system. The first step in the SiteClick™ antibody labeling process involves removal of terminal galactose residues from the heavy chain N-linked glycans using β-galactosidase, exposing essentially all possible modifiable GlcNAc residues. Second, the free terminal GlcNAc residues are activated with azide tags by enzymatic attachment of GalNAz to the terminal GlcNAc residues using the GalT(Y289L) enzyme. In the third step, the azide residues are reacted with the dibenzocyclooctyne (DIBO)-functionalized probe of choice (e.g., Alexa Fluor® 488 DIBO alkyne). The average degree of labeling is 3–3.5 labels per antibody.
Select the right tools for oxidative stress detection
Generation of reactive oxygen species (ROS) is inevitable for aerobic organisms, but it occurs at a controlled rate in healthy cells. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemia reperfusion injury, and neurodegenerative disorders.
Reagent kits are optimized for flow cytometry or for fluorescence microscopy and microplate analysis.
Imaging lipid peroxidation with the Image-iT® Lipid Peroxidation Kit. Human osteosarcoma (U2OS) cells were treated with 100 µM cumene hydroperoxide for 2 hr. A stain solution containing 10 µM Image-iT® Lipid Peroxidation Sensor and 2 drops/mL of NucBlue® Live ReadyProbes™ Reagent was applied for 30 min at 37°C. Cells were washed and imaged with Live Cell Imaging Solution.
Model systems for Parkinson's disease
Recently, Life Technologies has partnered with the Parkinson's Institute in Sunnyvale, California, to develop PD model systems using donor fibroblasts that have been collected at the Institute.
Flow cytometric assays for apoptosis
The mechanisms and controls of programmed cell death—apoptosis—are under intense scrutiny, because defects in apoptosis appear to be involved in a wide variety of human disease processes.
Reducing endogenous background with the Endogenous Biotin-Blocking Kit
A549 cells were fixed and permeabilized, treated (left) or not (right) with the Endogenous Biotin-Blocking Kit, labeled with a primary antibody against mouseanti-golgin-97 followed by DSB-X biotin–goat–anti-mouse IgG, then stained with Alexa Fluor ®488 streptavidin (green), Alexa Fluor® 647 phalloidin (magenta), andNucBlue® Fixed Cell Stain (blue). The image was acquired at 60x magnification on a Nikon® E800 upright microscope.
Highlight from BioProbes® Journal †
Live-cell staining of pluripotent stem cells: Alkaline Phosphatase Live Stain for early identification of induced pluripotent stem cells
In the Cell Signaling section of BioProbes 69, you will find the article "Live-cell staining of pluripotent stem cells". This article focuses on the Molecular Probes® Alkaline Phosphatase Live Stain, a fluorogenic alkaline phosphatase (AP) substrate that enables live-cell labeling of induced pluripotent stem cell (iPSC) colonies early in the reprogramming process, before immunocytochemical analysis is possible. When enzymatically turned over, this cell-permeant AP substrate produces a bright green-fluorescent product that then diffuses out of the cell, leaving behind no footprint.
AP activity has been shown to be up-regulated in pluripotent stem cells, including undifferentiated embryonic stem cells (ESCs), embryonic germ cells (EGCs), and iPSCs. Unlike other AP stains, neither the Alkaline Phosphatase Live Stain nor its enzymatic product accumulates in the cell. Alkaline Phosphatase Live Stain can be used to identify emerging iPSCs via robust fluorescent staining of the colonies as early as 14 days post-transduction, as well as to select iPSC colonies for expansion at 21 days post-transduction. Importantly, Alkaline Phosphatase Live Stain is nontoxic to emerging iPSCs and therefore can be used directly on master reprogramming plates to identify colonies for further propagation.
|Emerging iPSCs generated using the CytoTune®-iPS Sendai Reprogramming Kit. BJ human fibroblasts (ATCC) were transduced overnight using the CytoTune®-iPS Sendai Reprogramming Kit, and culture medium was replaced the next day. One week post-transduction, the cells were seeded onto inactivated MEF feeder cells in human PSC medium (DMEM/F-12 containing 20% KnockOut™ Serum Replacement and 4 ng/mL bFGF). At 14 days post-transduction, emerging iPSCs were analyzed for alkaline phosphatase activity using the Alkaline Phosphatase Live Stain, and images were collected on a Zeiss® Axiovert® microscope using a 10x objective: (A) phase-contrast image, (B) fluorescence image (using FITC optical filters), and (C) merged images..
† What's new with the BioProbes® Journal?
We are bringing our award-winning BioProbes® articles to you sooner. We will be publishing new BioProbes® articles online every month and highlighting those articles here. That way, we can keep you up-to-date on new fluorescence technologies and cell biology applications. Check back frequently and watch BioProbes 69 take shape!
Fluorophore selection in experimental design
Original broadcast: April 26, 2012
Choosing a fluorophore is one of the first important decisions to make when developing an experiment. A fluorophore is a compound that emits light at a specific wavelength when it has been excited at a lower wavelength. View the free webinar and explore:
- How to choose the best organic dye for an assay
- Quantum dots and how they compare to other dyes
- When to use a phycobiliprotein like R-PE or APC
- When to use a fluorescent protein like GFP
- How to choose a suitable dye to match your instrument
In addition, we explore the basic characteristics, strengths, and weaknesses of various fluorophores to help you develop the best assay for your needs.
An introduction to flow cytometric analysis using Molecular Probes® reagents, part I: Cell proliferation analysis
Original broadcast: November 21, 2011
In this free webinar, we discuss flow cytometric analysis of cell proliferation using CellTrace™ Violet and CellTrace™ CSFE, Click-iT® EdU, dual pulse labeling with EdU and BrdU, Vybrant® DyeCycle™ stains for live cell cycle analysis, and FxCycle™ stains for fixed cell cycle analysis.
© 2013 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. NanoDrop is a registered trademark of Thermo Fisher Scientific, Inc. Nikon is a registered trademark of Nikon Corporation. Zeiss and Axiovert are registered trademarks of Carl Zeiss AG Corp. Feel free to distribute ProbesOnline™ newsletter to friends and colleagues, but please keep this copyright statement intact.
* No purchase necessary. This promotion is only available to life science professionals 21 years or older in the US and Canada. Offer valid on requests received by Life Technologies no later than September 30, 2013, or until promotional supplies are depleted, whichever comes first. Limit 1 free copy per customer. Please allow 4–6 weeks for delivery. By accepting the Handbook, customers represent that they are not prohibited by law, regulation, or agency or institutional policy from receiving the Handbook from Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.


text:
I feel like I haven't posted in ages! I've had so much happening on the business side of my life, with lots of custom orders and my first experience with consignment in a brick-and-mortar shop. I also have been trying to stock my Etsy shop with new items.
But, "real" life goes on just the same. For example, the boys have been sick with the awful norovirus this past week. I've never done so much laundry, blech! On a funner note, our Spring Break occurred mid-April and my mom came to visit for a week! It was so wonderful to spend some quality time with her. The kids loved hanging out with Yaya and we sat down for some craft projects.
I had been wanting to paint some square wood panels to decorate our bathroom for a while. I took the opportunity of some rainy day crafting with my mom to get the paints out. Our bathroom walls are a nice shade of turquoise, while the floors are tiled in black in white. I thought some modern triangles featuring those colors along with others might be fun.
I first tried out some large triangles with my watercolors. I love how vibrant the colors turned out, but despite a couple of layers, you can see every brush stroke -- not sure I like that.
Before I went any further, I tried smaller triangles and gouache paint on my other wood panel.
What do you think?
I don't know whether to keep going, maybe add some additional colors -- a pop of red or fuchsia perhaps? -- or just paint over it all with a coat of white and start from scratch.


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Exhausted from three days of being on the run from police and wrapped up in a sheet of carpet, Jonathan Elbers was submissive when police confronted him in a hunting blind in northeast Chesterfield Township on Sunday afternoon.
'He just gave up,' Chesterfield Township Police Chief Bruce Smith said. 'He said something like 'I've had it, I give up.' I think we wore him out.'
Elbers, 23, of Tuscola County, was taken into custody without incident at 12:45 p.m. by Officer Frank Unger who found him hiding in the in the blind in a heavily wooded area north of Washington Road and east of Interstate 94. After being checked by medical personnel, he was questioned by police and then transported to the Macomb County Jail.
Elbers had been on the run since about 1 a.m. Friday after he allegedly broke into the home of a former girlfriend on Nicolette Street, bound her and tried to kill her.
Her parents, who were on vacation in Florida, called police to check on the 20-year-old woman because they were concerned about her safety after learning she was having trouble with Elbers after the two broke up.
Officers conducting a welfare check at the house were let inside by her sister. They walked through the dark house calling her name and heard the victim yell 'Help me' from the basement.
As the officers began walking down the stairs, a bleeding Elbers appeared. At some point, the woman was able to strike him in the head with a porcelain figurine, opening a cut on his head.
Elbers refused with the officers' commands and a struggle took place. Elbers was able to break free and fled outside on foot. They found the woman had been assaulted and bound, and Elbers was in the process of placing a large bag designed to cover a Christmas tree over her when police arrived.
Investigators found a rifle and duct tape inside of Elbers' truck parked near the house. The victim told police he had threatened to shoot her and then turn the gun on himself.
Officers searched throughout the night but were not able to locate the suspect. As daylight broke, they teamed up with a number of other law enforcement agencies to search the wooded areas north of the Nicolette Street residence on Friday, using canine teams and helicopters.
On Saturday, police received word about a home invasion in the area of 25 Mile Road and New Haven Road. The homeowners, who had been on vacation, returned home to find blood in the residence and it appeared someone had been sleeping in one of the beds. Police searched the area but found no sign of the intruder.
But Unger, the arresting officer, returned to the property on Sunday afternoon to check out a hunting blind. That's when he found the suspect, using the carpeting to either hide in or to keep warm as he slept overnight.
'The officer who found him just wouldn't give up,' Smith said. 'He started thinking about the deer blind and went back and asked the homeowner for permission to check the property again.'
Chesterfield Township police Sgt. Brad Kersten said the strong law enforcement presence -- which included the Macomb County Sheriff's Office, Michigan State Police and federal agencies -- kept the suspect pinned down in the area and made it difficult for him to escape over the weekend.
'All of that manpower gave us the ability to flood the area immediately,' Kersten said. 'I think if you could ask the suspect, he would say he couldn't leave because every time he turned around, there was a police car or a uniformed officer traipsing the neighborhood. He couldn't move. From the day we started chasing him, he didn't leave that wooded area.'
Word of the manhunt was publicized in television, radio, newspaper, and online news reports as residents were warned that the suspect was on the loose and considered to be highly dangerous.
As news of the arrest spread on Sunday, residents were able to breathe a sigh of relief, said Brian DeMuynck, a township trustee and retired sheriff's deputy.
'It's good for the community to know this subject is off the streets and we don't have to worry about him anymore,' DeMuynck said.
The Macomb County Prosecutor's Office has issued a warrant charging Elbers with assault with intent to murder, a life felony; first-degree home invasion, a 20-year felony; unlawful imprisonment, a 15-year felony; and assaulting a police officer, a 4-year felony.
He is expected to be arraigned on those charges on Monday.


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Now that my Warrior is hitting Naxx on a regular basis, is exalted with Hodir and has nearly all the goodies out of Heroic Instances that I had on his shopping list, I've been feeling the call of the wild, urging me to return to my Feral roots and level my Druid, Urago, who has been sitting idle in Shattrath City for many moons now. I wanted to be able to provide Bear Tank insights here anyway, and the best way to do that is to experience it first hand, right? So I've been hitting Howling Fjord the past couple of days, gathering Goldclover and killing Vrykul along with my Resto druid buddy Rivanu. So far we've not run into any major challenges with my AoE tanking abilities and his HoTs, we just keep hitting and healing until things sparkle. I've been doing a lot of experimenting with builds and here's what I've finally landed on:
I'll go through each of my choices here as well…
BALANCE TREE: There's really nothing beneficial for a Feral Druid to be found in here. Sorry Boomkins. Keep on dancin' on those mailboxes though.
FERAL TREE (60 pts): Go figure, the bulk of your points are going to be spent in this tree, whether you go for more of a Bear build, a Cat build or even hybrid between the two (as I've half-heartedly tried to do, though I admittedly slant far more toward Bear.)
Ferocity (5/5): Maul, Swipe, Mangle. These are your 3 biggest weapons when tanking, particularly when tanking multiple targets. Saving 5 rage each is a huge benefit, especially while levelling. In an end-game tanking setting where you're more likely facing infinite rage scenarios, I might not go this route. You also save energy on your Cat Mangle, which is great for DPS mode.
Feral Aggression (0/5): My standard build philosophy is Survivability > Rage Management, so why Ferocity over FA? I'm building for 5-man content mostly. If I ever raid, I might be more inclined to respec FA as given the 2 choices, FA clearly provides more survivability. But for my purposes, I would rather conserve Rage on my 3 most used abilities for the time being.
Feral Instinct (3/3): +30% Swipe Damage? Hellz yeah! AoE tank away baby.
Savage Fury (2/2): +20% Mangle Damage? Woot! Great for single target tanking OR DPS'ing, and combine w/ Berserk for AoE happiness. Mangle is our Shield Slam and we must give it love accordingly. You give a Warrior +20% to SS and they'll wet themselves with glee.
Thick Hide (3/3): No-brainer. You don't get block, you don't get parry, you don't get plate. Dodge, Armor and Health are all you have. Your armor multiplier no longer applies to bonus armor or rings/trinkets/etc. This is a bear-form must have.
Feral Swiftness (3/3): Remember what I just said about Dodge?
Survival Instincts (1/1): Bear Stand. Warriors are loaded with "OH S#1T!" buttons. We get one. Take it.
Sharpened Claws (3/3): Aside from being a prereq for a talent or two in tier 4 you might want, 6% added crit is great for threat and will be even greater for Mitigation (What? Yes!) if the current 3.1 PTR changes end up making the live 3.1 release.
Shredding Attacks (0/2) – Not so hot for Bear. If you're wanting to specialize in Kitty DPS then by all means (but you're on the wrong site, my fine feline friend.)
Predatory Strikes (3/3) YES PLEASE! It's also nice when they make 1 must have talent a pre-req for an even better must have talent. I wish whoever created the Destrolock tree felt the same way (Soul Leech Fail!)
Primal Fury (0/2) OH! Controversy abounds! No Primal Fury? Well, like I said, my build priority is Survivability > Threat Gen > Rage mgmt. Sure it could be argued that Rage mgmt. and Threat Gen. go hand in hand, but I think I'll get more bang for my buck out of where I spent these 2 points, which I'll get to much much later.
Primal Precision (2/2): 10 Expertise. NOT 10 Expertise Rating. 10 Expertise. I haven't looked.. I don't know how much Expertise we'll have available from gear, but if I can drop 2 talent points to give myself over 1/3rd of the Expertise I'll need at 80 to hit the Dodge cap and really cut down on parries? Sign me up.
Brutal Impact (2/2): More controversy! Some say this is a pvp talent. I say Bash now carries a SEPERATE interrupt component from the STUN component. So even if a mob is immune to Stun, they can still be affected by the interrupt. Interrupting spells is a crucial skill to have in my tanking opinion, and knocking 30 sec. off that cooldown is a big help.
Feral Charge (1/1): Gotta have it for those pulls where you want to keep the mob tanked right where he/she is. Plus.. you can use it in combat w/out the need for an additional Warbringer like talent, and you can fly by kicking it off in mid-air, which is great on knockback boss fights.
Nurturing Instinct (0/2): A cat-form only talent and even then I'm not sure I'd recommend it. MAYBE if you're a heavy pvp'er.
TIER 6 (The greatest tier in all of Feral Beardom!)
Natural Reaction (3/3): 6% MORE dodge. For those keeping track at home, that's a 10% Dodge increase through talents alone. Duh.
Heart of the Wild (5/5): 20% more STAMINA. That's not Health. That's STAMINA… the kind that scales with cool things like Blessing of Kings. There are no excuses for not having this.
Survival of the Fittest (3/3): Even with the upcoming 3.1 nerf, this is still a must have. If you don't know, we'll likely be losing a bit of the armor bonus here but getting it back in the form of a crit. based "shield" effect elsewhere. Even w/out the armor bonus, this is how we get to be uncrittable in 0 +DEF gear, so you have to have it anyway.
Leader of the Pack (1/1): Your DPS will love you. The aura from this is a really great boost and you'll be a hit in all your parties.
Improved Leader of the Pack (2/2): Now your healers will love you too. Think of it as a Replenishment buff for Green bars. You're going to want to crit on mobs anyway… why not get some health back? The mana gain is inconsequential except maybe in PVP.
Primal Tenacity (0/3): This USED to be really good in PvE when Fear was a massively devastating (and rather often utilized) mechanic. Now this is really more of a pvp talent and not worth picking up compared to what else is available.
Protector of the Pack (3/3) Survivability! Also note, it's no longer based on the number of people in your party, which was a wierd mechanic anyway.
Predatory Instincts (0/3): Again, a Cat thing. If I'd had more points, sure. It's nothing personal.
Infected Wounds (3/3): Nasty! It turns your Maul, Mangle or Shred (cat) into a sort of mini single target Thunderclap in addition to the damage they're already inflicting. Great debuff especially on those wacky hard-hitting melee boss types.
King of the Jungle (3/3): Here's where I spent those 2 points that I didn't put into Primal Fury. The damage boost while Enraged is 15%. That's really nice for when you're in a DPS race, getting a strong lead on Threat, or are just tired of staring at Boss Face and want to make it die quicker. Make sure your healer can keep up w/ the increased damage you take while enraged and you'll be fine.
Mangle: Dur. It's our Shield Slam. You're going to need this and you're going to want to keep its cooldown constantly spinning. Whatever rotation you use, this is your DPS AND Threat cornerstone.
Improved Mangle: I'm puzzled by the number of Druid Builds I see that exclude this. Even discounting the energy savings in Cat form(which is a nice bonus for those times when you have to DPS) you're taking one entire GCD out of your rotation in between Mangles. More Mangle uptime means more threat for you and more bleed debuff on baddies which is win all the way around. I can't imagine not wanting this.
Rend and Tear (5/5): 20% more Maul damage on a bleeding target. Here's the deal, unless you just ignore Lacerate altogether (which you shouldn't cause keeping 5 stacks of it going at all times w/ the Mangle debuff always on the mob really adds up fast) chances are REALLY good that somebody in your party is going to make the mob bleed, even if it's not you, even though it SHOULD BE. Maul is our Heroic Strike. In an infinite rage scenario, all your normal swings should be Mauls. If you glyph your Maul (which you SHOULD) to hit 2 targets, this becomes an even better tanking tool because it helps both single target and AoE.
Berserk (1/1): Mangle hits 3 targets and has no cooldown (GCD still applies) for 15 seconds. I don't think I need to explain how good this is.
Improved Mark of the Wild (0/2): Unless you're the only druid in your party, Restos nearly always pick this one up so you won't ever need to cast yours. If you ARE the only Druid in your party, it might be worth it.
Nature's Focus (0/3): Why are you healing? No. Bad Druid.
Furor (5/5): The days of powershifting may be gone but you've got to get in 5 points in this tier somewhere. Might as well be on something you'll actually use.
Naturalist (5/5): +10% Damage in Bear or Cat form. WIN!
Subtlety (0/3): Doesn't help you at all
Natural Shapeshifter (0/3): If you PVP a lot or are just so in love with Master Shapeshifter that you can't live without it, OK. Otherwise, pass.
Intensity (0/3): Used to be an OK pickup to boost your Enrage, but now your points are needed elsewhere.
Omen of Clarity (1/1): Still great for Feral despite the fact that they actually now made it work for Resto and Boomkin, too. If you're a MT Bear in an end-game, infinite-rage scenario, then you don't need this and you can put this point elsewhere. Otherwise, it's great and you want it.
Master Shapeshifter (0/3) I tested this one a lot. In the end, I was not blown away by the +4% Bear Damage increase. If you are focusing heavily on Cat DPS, then the 4% Crit booster is very appealing and you might want to go 15 into Restoration to get this talent along with OoC.
And there you have it, my thoughts on building a Feral Druid Tank (with some DPS thrown in for utility) in the world of 3.08. I'll discuss any 3.1 changes in the forums once they're official. Next up, my thoughts on Feral Tank Gear for lvl 80