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I bought 5 variations of these shoes in different colours, sizes and styles (ankle boot and court), but these are the only pair I have left now. They are pretty impossible to walk in and ache after about 1 minute, so I couldn't very well justify owning so many pairs that I would probably not get use out of. I love the bold colour of these and because my dress today is white and black, I felt it needed a splash of colour and between my hair and these shoes, I've certainly achieved that!
Green suede courts with statement heel Topshop Unique
Tuesday, 29 June 2010
Day Two Hundred & Fifty Seven - Brighten Up
Posted by Pink Haired Princess at 6:18 pm
2 comments :
Thank you for your shoeaholic love!!
Unfortunately I've had to disable anonymous comments for now, due to a high volume of spam.
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Risk factors:
1. Age (not seen in men < 40 years old; incidence increases with age; 60% of men >.80 have prostate cancer)
Patients present late, because early prostate cancer is asymptomatic. Look, for symptoms suggestive of benign prostatic hypertrophy (hesitancy, dysuria, frequency) with hematuria and/or elevated prostate-specific antigen (PSA) or acid phosphatase. Acid phosphatase is elevated only when the cancer has broken through the capsule; for this reason, it was replaced with the more sensitive PSA as a screening tool. Look for prostate irregularities (nodule) on rectal exam. Patients also commonly present with back pain from vertebral metastases (osteoblastic).
Local prostate cancer is treated with surgery (prostatectomy). Patients with metastases have several options for hormonal therapy: orchiectomy, gonadotropin-releasing hormone agonist (leuprolide), androgen-receptor antagonist (flutamide), estrogen (diethyistilbestrol), and others (e.g., cyproterone). Chemotherapy does not work, and radiation therapy is used for local disease or pain from bony metastases.
Was this article helpful?
Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

subset: commoncrawl


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Importers and the Lagos Chamber of Commerce and Industry (LCCI) have faulted the revenue targets given by the Federal government to the Nigeria Customs Service (NCS), Federal Inland Revenue Service (FIRS) and other government agencies at ports.
One of the importers and a member of the group, Chief Michael Adejare said there were negative manifestations in the manner of import valuation by the Customs.
"Reports reaching the chamber indicate many instances of upward review of values of imports in complete disregard to the values of invoices of such imports.
"Importers have been made to pay import duty and other charges that are far beyond what they ordinarily should have paid. Many investors have suffered untold hardship as a result of this practice, especially when there is no effective dispute resolution system in place," he said.
Adejare said the idea of giving targets to revenue-generating agencies could result in some unintended consequences.
In view of dwindling crude oil revenue, The NCS and the FIRS, have been given targets.
Another importer, Dr Badmus Solomon said there is a risk that best practice principles will be compromised by the agencies in their desperation to meet targets.
He noted that the downward trend of oil prices in the international market is a setback for the economy.
Solomon said the scenario of sliding oil price has implications for the capacity of the government to meet their statutory obligations.
He said: "On top of that, we are struggling with the problem of crude oil theft, which is taking its toll on output. For an economy that is 95 per cent dependent on oil for its foreign exchange earnings and 85 per cent dependent for revenue, this development should be a cause for concern," he said.


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Health Professionals for Clean Air and Climate Action
Medical professionals are on the front lines taking care of patients experiencing the direct impacts of air pollution and climate change. From higher ozone and particle pollution levels due to increased heat and wildfires—to threats from severe storms and the spread of vector-borne diseases—the science is clear; climate change poses one of the greatest threats to lung health.
Thank you for joining us in our mission to protect the air we breathe and ensure a safe climate for future generations. Here are some important ways you can make a difference:
Sign up for our health professionals' newsletter
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Get opportunities and updates in the fight for healthy air in our newsletter specifically for health professionals.
Thanks for joining us in the fight for healthy air and healthy lungs
We look forward to sharing the latest lung health news with you.
Stop Censoring Science
The U.S. EPA has proposed to censor the science it uses in its decisions—and ignore research that shows that air pollution harms health. Tell the Trump Administration not to censor science.Sign the petition
Declaration on Climate Change
Join thousands of health professionals nationwide by signing this statement in support of urgent action to protect the health of patients and communities from the impacts of climate change.Sign the declaration
We're collecting stories from health professionals and patients on the impact of air pollution and climate change on health.Share your story
Health Professionals in Action
Health professionals are stepping up to support urgent action to address air pollution and climate change.Learn more
Climate Change and Health
Climate change seriously threatens our wellness—especially our lung health.Learn more
The resources below can assist health professionals with opportunities to take action and advocate for patients, health and climate health.
Health Organization Climate Toolkits
- Alliance of Nurses for Healthy Environments – Nurses Climate Challenge
- American Academy of Pediatrics: Climate Change and Children's Health
- American College of Physicians – Climate Change Toolkit
- American Public Health Association – Climate Change
- ecoAmerica – Research Resources
- Health Care Without Harm – Health Care Climate Challenge
- Medical Society Consortium on Climate Change and Health
- National Association of County and City Health Officials – Climate Change
- Public Health Institute Center for Climate Change & Health – A Physician'''s Guide to Climate Change, Health and Equity
- US Climate and Health Alliance – Advocacy Tools for Health Professionals


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E-Commerce as we can understand by the word itself that the business which is done completely online where sellers can sell their products and customer can purchase a product according to their needs and requirement without any paper work that is called E-Commerce.
In E-commerce solution DVS Web Infotech mastered their employees to work on according to latest technologies. We are providing a wide range of E-Commerce Solutions. You can have your own E-commerce portal to sell out your product or anything online without any problem. We are using latest technologies to secure your portal at any cost. We provide integrated Pay-Pal system for online transaction


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GREENSBORO, N.C. – The Franklin & Marshall baseball team opened its 2014 season with a back-and-forth 14-9 victory on Saturday afternoon in non-conference action. The Diplomats sit at 1-0, while Guilford fell to 6-4.
The Quakers jumped out in front with an early 4-0 lead after tagging F&M for four hits in the first, which included a pair of doubles in the frame. The Diplomats answered in resounding fashion, putting eight runs on the board off of eight hits in the top of the second.
Will Benenson and Joe Gallitto opened the inning with back-to-back singles before Pat Andrews reached on an error to load the bases. Luke Seib followed with a two RBI single before Brett Poniros stepped up to the plate and laced a two RBI single of his own to center.
Rob Anderson and Matt Mezansky continued the onslaught with RBI singles before Benenson doubled to left field to plate his second runner of the inning and Gallitto wrapped up the scoring with his second hit of the stanza, an RBI single to right field that scored Benenson.
Guilford cut into the deficit with an RBI single in the bottom of the second to make it an 8-5 game and continued the comeback bid with a bases-clearing grand slam in the bottom of the fourth to take the 9-8 lead.
However, F&M responded once again when Anderson scored two off a single up the middle in the top of the fifth to put the score in favor of the Diplomats at 10-9. F&M held onto the lead from there on out, adding three more runs in the top of the seventh off an RBI single from Casey Bulik and a two RBI double from Mezansky, before tacking on another run in the ninth to cap off the scoring.
F&M tallied 19 hits on the afternoon, compared to 17 from the Quakers. Gallitto led the way with a 4-for-6 effort, while reigning Centennial Conference (CC) Rookie of the Year Mezansky went 3-for-6 with four RBIs. Anderson went 3-for-5 from the leadoff position and scored three runs while tallying three RBIs.
Alex Guerra (1-0) picked up the win in relief of starter Sean Cosgriff. Guerra entered the action in the fifth and shutdown Guildford, allowing seven hits and no runs in four innings of work. John Todd (1-1) was charged with the loss after surrendering three runs on five hits in 3.1 innings on the mound.
The Diplomats return to the field tomorrow for an 11:00 a.m. meeting with Case Western Reserve. The non-conference tilt once again takes place in Greensboro, N.C., hosted by Guilford.


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|Members of the ethnic minority arrive in Thailand from Myanmar and Bangladesh in hope of better lives, but enter a tangled human trafficking web where local officials, complicitous members of their own group and fixers make them pay dearly for their dreams.
Muh, a 43-year-old Rohingya living in Bangkok for over a decade, has intimate knowledge of trafficking operations originating in either Bangladesh or Myanmar's Rakhine state, where stateless Rohingya have been subjected to violent persecution in recent months.
Before the raid on 400 Rohingya near the Thai-Malaysian border in Songkhla's Sadao district on Thursday, Muh, real name withheld, told Bangkok Post Spectrum his connections had informed him that security officials were preparing to pounce on a ''safe house'' there.
He painted a complicated picture of the smuggling racket, involving complicitous Rohingya in both countries, corrupt Thai officials, fixers throughout the whole process and safe houses where the Rohingya are kept until they can be smuggled across the border.
While smugglers can demand up to 50,000 baht [US $1,1667] for passage to another country, costs can go up considerably for Rohingya detained on Thai soil.
Muh says in some cases Rohingya who are reportedly deported back to Myanmar are in fact taken to safe houses in southern Thailand.
The smuggling boats can carry 50-80 people per trip and the captain is usually fluent in Thai, Myanmar and Bengali.
''These people want to be arrested by Thai officials because it is part of the plan,'' Muh said. ''Once they are arrested, they go through the immigration process.
The trafficker gets involved during the deportation process. This is the point when they have to pay another sum so that they can go to their preferred destinations. Those who can afford to continue their journey will get to go to Malaysia or Indonesia. But those who remain here will be sold to the fishing industry where they will be forced to work for 20 hours a day with no days off. I can't reveal where, but sometimes Rohingya are sold to fishing boats in the middle of the sea.''
Surapong Kongchantuk from the Lawyers Council of Thailand chairs the human rights subcommittee on ethnic minorities, stateless people, migrant workers and displaced persons and has been documenting and working on cases involving smuggled Rohingya for over a decade.
In the past, the Rohingya had come to Thailand by themselves to escape poverty and persecution, but trafficking has become big business in the last four to five years.
''A Rohingya agent will go around and tell them about coming to Thailand and then going on to Malaysia for work for good pay,'' he said.
''The agents are Rohingya who either live in Thailand or Malaysia. Once people in the village see them having a good life in other countries, they want the same.''
Mr Surapong said the Rohingya coming from Myanmar pay 300,000-700,000 kyat (10,600-24,800 baht) to the smugglers and those from Bangladesh 20,000-28,000 taka (7,600-10,600 baht) [30 baht = US $1]. The journey to Thailand usually takes two weeks by boat.
''They used to destroy the boat and run away, but now they surrender to police,'' Mr Surapong said. ''The trafficker takes care of them from that point on.''
''If they want to go to Malaysia, they will have to pay 1-1.5 million kyat or 50,000-80,000 taka. If they don't have the money up front, they can choose to have it deducted from their monthly salaries.''
Mr Surapong said according to interviews with Rohingya, some had their passage paid by relatives living in Thailand, but those who didn't have enough money were sold into slavery, sometimes, as Muh pointed out, in the middle of the sea.
Those who can afford the pay-offs are taken to the safe houses where additional payments determine how quickly they can be moved across the border. Sometimes relatives again come to the rescue; at other times the Rohingya are expected to work to pay off their debts.
''Safe houses are located in many locations, especially in Hat Yai district and Padang Besar district of Songkhla province and Sungai Kolok district of Narathiwat province,'' Mr Surapong said.
''All of the safe houses are located in the jungle where no one can easily see them. The safe houses are protected and taken care of by people in 'coloured uniforms'.''
Department of Special Investigation anti-human trafficking centre chief Pol Maj Jatuporn Arunreukthawil said the involvement of Thailand-based Rohingya in the trafficking of their own people had become a concern.
''For the entire time I have been monitoring the issue, I have noticed the majority of Rohingya who came into Thailand are male. But lately, there are some females and children coming in,'' he said.
Pol Maj Jatuporn said by law it was an issue for immigration police to handle, not the military.
''I have heard of many suspicious things going on down South. I have all the information, but I can't reveal it because it will interrupt cases that we are working on at the moment.''
Mr Surapong said despite the best efforts of the Thai government to deal with the issue in a humane way, more and more Rohingya were coming. ''Part of it is because they are treated well once they arrive here,'' he said. ''They get food, water, gas and other things that they cannot get from Myanmar.''
Records detailing the number of Rohingya arrested or detained upon arrival in Thailand date back to 1998. That year, 104 were detained upon arrival after crossing overland into Tak province. That number climbed to 1,123 in 2006, 2,763 in 2007 and 4,880 the following year.
In 2009, the number of arrests dropped to 93 following international publicity about Thai officials pushing the Rohingya's boats out to sea. However, the following year that number rose dramatically to 2,350.
''These numbers reflect the number of people arrested; there are still many of them who were not on the record,'' Mr Surapong said.
The military's Internal Security Operations Command (Isoc) is responsible for national security issues, including Rohingya who try to enter the country illegally. In 2008, Isoc set out guidelines in its Standard Operating Procedures (SOP) to handle the illegal immigrant problem along Thailand's borders.
Spectrum contacted Isoc Region 4 officials in Pattani and Ranong provinces, but both declined to comment on the Rohingya issue, and referred us to Isoc's main office in Bangkok. No one in Isoc's Bangkok office was available for comment.
Another problem for Thai officials is whether to designate Rohingya as Myanmar nationals when they are deported.
On Jan 1, 73 Rohingya travelling by boat were detained at Koh Bon and taken to Phuket immigration office. They were designated as Myanmar nationals and deported across the border at Ranong the following day.
''The reason that we have to document them as Myanmar is because we are afraid that we won't be able to deport them at the border if we document them as Rohingya since they are not recognised as citizens by the Myanmar government,'' said one immigration police officer.
Phuket Governor Maitri Inthusut, who claimed he has full authorisation to deal with illegal immigrants, said Rohingya were treated in a gentle, humanitarian way because of the difficult situation they were in.
''Basically, I just gave them what they needed,'' he said in reference to the Bon island group. ''They had travelled for almost two weeks. They had no food left by the time they arrived, so we offered them some. They said they wanted to travel further. We thought about giving them the petrol to continue their journey to Malaysia, but the sea was really rough on that day. Moreover, their boat was not in good enough condition for them to travel that far.
''Letting them continue would be the same as trying to kill them, so I ordered the officials to take them to land and follow the standard procedure. For this case, we had no choice but to deport them. I understand that they are seeking asylum, but we are not in the position to handle that.''
The governor said while it may appear to be a big national security issue involving human rights, Phuket had to consider its image as a tourist town.
''There is no way that we would keep a warship along the coast to guard our border, instead we are acting as a good housekeeper who keeps the whole town clean and organised,'' he said.
Nightmare without borders
After a week-long trek, Muh arrived in Thailand from Myanmar 13 years ago hoping his ordeal under the repressive state regime was finally over.
Originally from Rakhine state, Muh, who unlike many Rohingya come by boat, arrived on foot via the Mae Sot border in Tak province with two friends. A new chapter in his difficult life was about to begin. ''I ran away from my village and headed towards Yangon. The only safe place I could think of was Thailand,'' said 43-year-old Muh.
''Once we crossed the Thai border, we felt safe and didn't have any further plans of where we wanted to go. The only thing that scared us was being deported back to Myanmar, because I knew in my heart that we would end up back in prison.
''Our worst fears came true when we were arrested by Thai police in Tak province. We were sent to a prison that held many others from Myanmar. We were mixed in with them for a couple of day before three big trucks—not police trucks—took us somewhere we didn't recognise.
''There were 30 people on each truck. I was separated from my friends, and scared of where we'd end up. Then we arrived at a safe house where a man explained that if we didn't want to be deported back to Myanmar, we would have to pay them money. Then they would take us to work in Thailand.
''I ended up paying around 700 baht. It was a lot of money for me at the time—I had almost nothing. Then they brought me to a buffalo farm in Tak province. I watched and fed the animals, and slept in a small shack. I got paid 200 to 300 baht a month with food and a place to sleep.
''After a while I ran away. I just took any bus and got off at Nakhon Sawan, where I found a family who helped me. I got a job as a construction worker before I went to Nakhon Ratchasima province. I saved some money and decided to come to Bangkok with a little help from my Rohingya friends.''
In Bangkok, Muh made a living selling roti on the street. Soon he was able to communicate in Thai fluently. His language abilities and connections make him an ideal interpreter when Rohingya arrive in Thailand through human traffickers.
Muh says he declines to get involved with traffickers who smuggle Rohingya to sell in the labour market, but he admits that such smugglers exist all over Thailand. He knows about much of the movements of Rohingya into Thailand.
''None of the Rohingya want to come to Thailand. Their main target is to make it to Malaysia or Indonesia. Thailand is just the passageway for them to get to their desired destination.
''When we see news of Rohingya coming to Thailand by boat, most are younger males. That's because the traffickers want to sell people for labour.
''Each of them contacts a local agent from Myanmar or Bangladesh. Then the Rohingya have to pay the agent to get them on a boat sailing to the Thai border. It usually takes 10 to 14 days, and they normally end up in Ranong, Phangnga or Phuket. It depends on where the agent wants them to be.''
Still the memories of the oppression his people face at home still hang heavily on Muh.
''The threat of being raped, robbed or killed were some of the worst nightmares we faced. Maybe that's why I don't know what a bad dream is because we faced it on a daily basis, every waking moment of our lives.
''The Rohingya are treated so unfairly. We have a long history of having roots in Myanmar, but we've never been included as part of the country. The military treats us as less than human. They can just come to our village and demand to take some women for their own pleasure. If we fight back, they'll hurt us. They're fully armed. So we have to trade our food, oil or money to save the women.
''So you can see why Rohingya want to escape from that nightmare and find a better place. They'll do anything to get away so they don't have to suffer.
''Some Rohingya travel by foot and sneak into Thailand across the border but most of them don't make it. Other popular options are to pay for someone to get them out of there or to sell themselves to a human trafficker.''
This article was first published in The Bangkok Post on January 13, 2013.


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I have been doing a lot of thinking these past couple months… well, more like weeks about the ability to change yourself in an attempt to "help" others change. Sound weird? Ya, I know. Here's the deal: You can't change how someone treats you. You really can't change anything about someone else. What you CAN do, is change who YOU are as a person, and that itself, in return allows others to treat you differently/better etc…
Lemme give you an example. Say there is someone in your life who is constantly making digs at you or just cuts you down all the time. There is a multitude of ways to handle this. One, you can do as I have in the past and return all of the snide remarks and digs with even more of my own to further escalate the bullshit. Which, in my personal opinion, after much experience with it, SUCKS ass. However, I believe I have "seen the light" if you will, about how to handle people in my life. I have made personal changes within myself to assist others in how to treat me. First, I can see that it has helped a lot, BUT it has helped me (directly) as well. I am slowly learning to control my OWN temper and not have such a short fuse. This is very good for me. I am also learning to hold my tongue. THAT is huge.. apparently, not everyone wants to know my opinion… and what I feel I need to say doesn't always matter.
So, I've noticed in people around me, that since I am trying to change me… that in turn is slowly changing them as well, and how they treat me. It's not fun to make digs at someone and not get anything back from them.. that gets old and boring, and thus they stop. I know, rather elementary school-ish, but hey.. it's working and I'm happy.
"Live the life you love…. "


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Are you sure you want to change country and/or language?
When you change country, all the products will be removed from your shopping cart.
U kunt dit product eventueel wel online bestellen.
Eradicate aphids? HGX spray against aphids is a unique product against aphids. It is a fast-working anti-aphid spray for eradicating aphids on houseplants and potted plants. Eradicate aphids quickly and effectively with HGX spray against aphids!


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CheckPoint Implied Rules
Implied Rules:
Apart from rules explicitly defined by an administrator, the Security Gateway also creates implied rules,
which are derived from Global Properties definitions. Implied rules enable certain connections to occur to
and from the gateway using a variety of different services. The firewall places implied rules either first, last,
or immediately before last rule in the Rule Base.
Examples of implied rules include rules that enable Security Gateway control connections and outgoing
packets originating from the Security Gateway.
To view implied rules:
1. Add at least one rule to the rule base.
2. Click View > Implied Rules.
The Firewall tab displays the Implied Rules in addition to the user-defined rules.
Order of Rule Enforcement:
The Security Gateway inspects packets and applies rules in a sequential manner. When a Security Gateway
receives a packet from a connection, it inspects the packet and applies the first rule in the Rule Base, then
the second rule and so on.
Once all elements in a given rule match the information contained the packet (source, destination, service,
etc.), the Security Gateway stops the inspection and immediately applies that rule. If no applicable rule is
found in the Rule Base, the traffic is automatically blocked.
It is essential that you understand the concept of rule processing. The firewall always enforces the first
matching rule to any given packet. This may not necessarily the rule that best applies to the traffic.
It is important to carefully plan your Rule Base and place rules in the appropriate order. The best practice is
to put rules that apply to very specific conditions at the beginning of the Rule Base. General rules should be
put toward the end of the Rule Base.
Rules are processed in the following order:
1. First Implied Rule: This rule cannot be modified or overwritten in the Rule Base. No rules can be
placed before it.
2. Explicit Rules: These are administrator-defined rules, which may be located anywhere between the first
and the next to last implied rules.
3. Next to Last Implied Rules: These are more specific implied rules that are applied before the last
implied rule is enforced.
4. Last Implied Rule: This is the default rule, which typically rejects all packets without logging.
Example Access Control Rule:
The following screen shot shows a typical access control rule, as seen in the Firewall tab of
SmartDashboard. This rule states that HTTP connections that originate from the branch office that are
directed to any destination, will be accepted and logged.
Figure 1-2 Typical access control rule
Special Considerations for Access Control:
This section describes Access Control scenarios.
The key to effective firewall protection is a simple Rule Base. One of the greatest dangers to the security of
your organization is misconfiguration. For example, a user may try to sneak spoofed, fragmented packets
past your firewall if you have accidentally allowed unrestricted messaging protocols. To keep your Rule
Base simple, ensure that it is concise and therefore easy to understand and maintain. The more rules you
have, the more likely you are to make a mistake.
Basic Rules:
When creating rules, ensure that you allow only traffic that you want. Consider traffic initiated and crossing
the firewall from both the protected and unprotected sides of the firewall.
The following basic access control rules are recommended for every Rule Base:
- A Stealth Rule to prevent direct access to the Security Gateway.
- A Cleanup Rule to drop all traffic that is not permitted by the previous rules. There is an implied rule that
does this, but the Cleanup Rule allows you to log such access attempts.
Remember that the fundamental concept behind the Rule Base is that actions that are not explicitly
permitted are prohibited.
Rule Order:
Rule order is a critical aspect of an effective Rule Base. Having the same rules, but putting them in a
different order, can radically alter the effectiveness of your firewall. It is best to place more specific rules first
and more general rules last. This order prevents a general rule from being applied before a more specific
rule and protects your firewall from misconfigurations.
Topology Considerations: DMZ:
If you have servers that are externally accessible from the Internet, it is recommended to create a
demilitarized zone (DMZ). The DMZ isolates all servers that are accessible from untrusted sources, such as
the Internet, so that if one of those servers is compromised, the intruder only has limited access to other
externally accessible servers. Servers in the DMZ are accessible from any network, and all externally
accessible servers should be located in the DMZ. Servers in the DMZ should be as secure as possible. Do
not allow the DMZ to initiate connections into the internal network, other than for specific applications such as UserAuthority.
X11 Service:
The X11 (X Window System Version 11) graphics display system is the standard graphics system for the
Unix environment. To enable X11, you must create a specific rule using the X11 service. If you select Any
as the Source or Destination, the X11 service is not included because when using the X11 service, the
GUI application acts as the server rather than the client.


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News Archive
ZESTRON Integrates a Video Teleconferencing System as Part of its
Manassas, VA - April 13, 2010 As an expansion of ZESTRON's previous substantial investments in its customer support infrastructure, the worldwide leader in precision cleaning products and services, has recently installed a high definition video teleconferencing system.
In January 2010, ZESTRON announced a comprehensive "Global Link" initiative focusing on the creation and seamless integration of the company's global knowledge database and technical expertise. With the goal of creating a borderless engineering environment, this initiative will allow customers to benefit from enhanced customer access - globally and locally.
The top-of-the-line Tandberg video teleconferencing system features full HD video quality at 1080p and opens the doors to cutting-edge visual communication. It is designed to offer ZESTRON's customers live and "hands-on" access to any of the company's worldwide engineering teams, especially in critical situations when travel is not an option.
"Our comprehensive approach to analyzing and solving high precision cleaning challenges, combined with our global technical expertise, allows us to deliver great value to all current and future customers," says Dr. Harald Wack, President, ZESTRON.


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[ vee-din ]
/ ˈvi dɪn /
a city in NW Bulgaria, on the Danube River.
Dictionary.com Unabridged Based on the Random House Unabridged Dictionary, © Random House, Inc. 2020
Examples from the Web for vidin
Prince Charles himself fired the first shot at Vidin, and his gallant troops followed him on to victory.The Passing of the Turkish Empire in Europe|B. Granville Baker
Therefore the outraged Sultan sent against him three armies in succession, and each of them went back from Vidin vanquished.The Birth of Yugoslavia, Volume 1|Henry Baerlein
He adorned Vidin with handsome buildings, maintained order, levied taxes and issued a separate coinage.
The fall of Turnovo was followed by the fall of Vidin and Dobrudzha.Area Handbook for Bulgaria|Eugene K. Keefe, Violeta D. Baluyut, William Giloane, Anne K. Long, James M. Moore, and Neda A. Walpole


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Add to history
Santa Ruiz was once voted the most desirable place to live in America. That was before the murder and kidnapping of twin high school girls. Now the town's secrets will be exposed. There are 3 powerful families: 1) Steele- Bethany Steele Tisha Steele 2) Wilson- Dr. Tom Wilson Grant Wilson Chad Wilson 3) Rhodes- Brenda Rhodes Russell Rhodes Kayla Rhodes Peter Rhodes


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United States Agriculture Secretary Sonny Perdue has branded the UK's decision to maintain tariffs in a no-deal Brexit 'disappointing'.
Abi Kay and John Wilkes report...
Defra Secretary Michael Gove told NFU conference this week the Government would keep tariffs on food imports such as sheepmeat, beef, poultry, dairy and pigmeat if the UK leaves the EU without a deal.
The decision, which is due to be formally announced on Monday when the rates are published, was made in spite of pressure to unilaterally drop tariffs from Brexiteers, think tanks and, according to reports, some Cabinet Ministers.
Responding to a question from Farmers Guardian at a Washington press conference, Mr Perdue said: "I have not heard [the announcement] or seen [it]. We were hoping to have a good relationship with the UK, assuming they Brexit.
"We have worked on that, we have had conversations about that, it would be very disappointing if that comes about.
"I think [Mr Gove] would follow the EU model, which I do not think is very helpful to the UK or the international market place."
Mr Perdue's comments follow a visit to the US by former Brexit Secretary David Davis, ex-Defra Secretary Owen Paterson and director of international trade at the Institute of Economic Affairs, Shanker Singham, late last year.
The trio shocked the UK agricultural community by using their trip to lobby for a trade deal which would be, according to Mr Davis, 'beneficial to American farmers'.
Vicki Hird, food and farming campaign co-ordinator at Sustain, described Mr Davis' comments at the time as 'an outrage'.
"David Davis was a Government Minister until this summer and yet is so desperate for a hard Brexit that he would throw hardworking UK farmers under a bus in favour of improving trade conditions for their American counterparts," she said.


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Hy members,
i think it's a shame that people could get community reward points for posting useless things in the forum like a smiley or something else.
We should enable a admin only for proofing posts.
So that it is impossible to get points for doing nothing.
We should act asap.
Sincerly, bitsurfer


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Crosses, Shadows, St. Francis Church
Crosses and shadows at the St. Francis Church, Ranchos de Taos, NM. Finding the light and darks at any religious landmarks, to me represents the symbolism of the eternal fight between the two. I mostly enjoy the architecture of the buildings and the people who built them. Thanks for looking. G
Red, Shadows, St. Francis Church
Red, Shadows, St. Francis Church. A lady in red leaving church in the shade of the morning shadow from the bell tower. The things and sites we see on a photo tour around Taos, the Gorge and beyond. Thanks for looking. G
Reflections At The San Francisco de Asis Church
Reflections at the San Francisco de Asis (St. Francis) Church, Ranchos de Taos New Mexico. I made this image on a photo tour this weekend with my wonderful guests from Minnesota. Early in the morning, before the groundsman/caretaker swept up the puddles following this Fridays rain, the reflections in the water amongst the flagstones were plentiful and offered up a multitude of photo opportunities. Thanks for looking. G


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KM-HN-1, MAGE-C1, MAGE-A3/6/12, MAGE-A5, MORC, DDX43, SPACA3, SSX-4, GAGE-1–8, and MAGE-C2, a combination of at least three CT genes—desirable for circumventing tumor escape mechanisms—is obtained in the MMC of 67% of the patients. Provided that the immunogenicity of the products of these 10 CT genes is confirmed, gene expression profiling could be useful in identifying which CT Ags could be used to vaccinate a given patient.
Multiple myeloma (MM)3 is a B cell neoplasia characterized by the accumulation of malignant plasma cells in the bone marrow. Although complete remission could be achieved in ∼25–50% of newly diagnosed patients treated with high-dose melphalan and autologous peripheral blood stem cell transplantation (ABSCT), almost all patients will relapse with a median of 2 to 3 years of event-free survival (EFS) (1). There is ample evidence that a small fraction of MM cells (MMC) escape chemotherapy and remain present even despite a "complete remission" (2). The remaining MMC are promising targets for active or passive immunotherapy because: 1) MMC can be lysed by CTL in vitro (3, 4, 5, 6); and 2) allograft strategies have shown that donor T cells can efficiently lyse MMC in some patients in vivo, provided that an efficient immune response is mounted (7, 8, 9). The clonal Ig produced by MMC seemed to be an ideal and specific target, but several vaccination trials using it failed to prove a clinical benefit for patients with MM (10, 11, 12), contrary to its promise in patients with B cell lymphoma (13). An explanation suggested by murine models (14) could be a deletion of the T cell repertoire related to the tumor Ig due to the large amounts of circulating tumor Ig.
In melanoma and epithelial cancers, several tumor-associated Ags have been identified (15, 16, 17). These Ags may be shared with normal cells, as MART-1, gp100, or tyrosinase. Another category, the cancer-testis (CT) Ags, is expressed by testis cells and malignant cells mainly from patients with melanoma, small cell lung cancer, hepatocellular carcinoma, or bladder cancer (18). To date, 47 CT gene families that include 93 genes have been described (19, 20, 21, 22). Strictly testis-restricted genes that are expressed only by male germ cells and malignant cells, represent about one-half of CT genes (19). These testis-restricted genes, in particular genes belonging to the MAGE, GAGE, and SSX families, are mainly located on chromosome X. Because of their restricted expression to germ cells and malignant tissues, there should be no deletion of a high-affinity T cell repertoire and theoretically a low risk of preexisting immune tolerance. Thus, the testis-restricted CT Ags are being used as targets in several vaccination trials (23). In particular, NY-ESO-1 and CT Ags belonging to the MAGE and GAGE families have been shown to elicit spontaneous cellular and/or humoral immune responses in cancer patients (24, 25, 26, 27, 28). According to the definition of Scanlan et al. (19), about one-fourth of CT genes are tissue restricted, i.e., expressed in two or fewer nongametogenic tissues, one-fourth are differentially expressed, i.e., expressed in 3–6 nongametogenic tissues but at a low level compared with testis, and five genes initially reported as CT genes are ubiquitously expressed.
No study has assessed the expression of CT genes on a pangenomic base in MM until now. Using DNA microarrays, we and others have shown that genes belonging to the MAGE and SSX families and NY-ESO-1 were aberrantly expressed in a certain percentage of primary MMC (29, 30). The expression of NY-ESO-1 has been correlated with a poor prognosis and its frequency is increased in patients displaying genetic abnormalities determined by conventional cytogenetic analyses (31). CT7/MAGE-C1 and MAGE-A3/6 protein expression correlated with elevated MMC proliferation (32). A recent study (28) reported the presence of functional CD8 T lymphocytes against NY-ESO-1, LAGE-1, and MAGE-A1,-A2, -A3, and -A4 in the peripheral blood of MM patients. Their frequency was 3-fold increased compared with that in healthy donors, supporting an immunogenicity of CT Ags in MM patients (28). In addition, van Rhee et al. have shown that anti-NY-ESO-1 CD8 T cells of MM patients were able to kill primary myeloma cells (31).
The aims of the present study are: 1) to assess the expression of CT genes on a pangenomic base in MM; 2) to estimate their prognosis value; and 3) to provide appropriate selection strategies for clinical vaccination trials.
Materials and Methods
Patients and cell samples
MMC were purified from two independent series of 64 patients with newly diagnosed MM (median age, 59 years) after written informed consent was given in agreement with French or German laws. Forty-seven of 64 patients and 64 of 64 of the first and second series, respectively, were treated with high-dose chemotherapy (HDC) and ABSCT. According to Durie-Salmon classification, in the first series 11 patients were of stage IA, 11 of stage IIA, 39 of stage IIIA and 3 of stage IIIB. Twelve patients had IgAκ MM, seven IgAλ MM, 24 IgGκ MM, 10 IgGλ MM, six Bence-Jones κ MM, three Bence-Jones λ MM, and two nonsecreting MM. In the second series, four patients were of stage IA, 11 of stage IIA, 45 of stage IIIA, and four of stage IIIB. Nine patients had IgAκ MM, three IgAλ MM, 20 IgGκ MM, 19 IgGλ MM, seven Bence-Jones κ MM, four Bence-Jones λ MM, and two nonsecreting MM.
XG-1, XG-2, XG-3, XG-4, XG-5, XG-6, XG-7, XG-10, XG-11, XG-12, XG-13, XG-14, XG-16, XG-19, and XG-20 human myeloma cell lines (HMCL) were obtained and characterized in our laboratory (3, 33, 34, 35). SKMM, OPM2, LP1, U266, and RPMI8226 HMCL were purchased from American Type Culture Collection (distributed by LGC Promochem). Normal testis RNA samples were purchased from CliniSciences. Bone marrow samples were obtained from healthy donors and patients after informed consent was given in agreement with French or German laws. Normal bone marrow plasma cells (BMPC) and primary MMC were purified using anti-CD138 MACS microbeads. Briefly, mononuclear cells were collected by centrifugation on a Ficoll-Hypaque cushion, washed twice, and incubated for 30 min at 4°C with B-B4 anti-CD138 MACS microbeads (Miltenyi Biotec). Cells were then washed and purified using the autoMACS device (Miltenyi Biotec) according to the manufacturer's instructions. The purity of plasma cells in the sorted population was determined by labeling autoMACS-sorted cells with PE-conjugated anti-CD138 mAb (Beckman Coulter) and FACS analysis (FACScan; BD Biosciences). This sorting procedure generally insures a high plasma cell purity (≥95%). Sorted MMC were discarded if the purity was >95%. For the isolation of peripheral blood memory B (MB) cells, monocytes, NK, and T cells were first removed using anti-CD14, anti-CD16, and anti-CD3 magnetic beads (Dynal), and MB cells were then positively selected using anti-CD27 MACS microbeads (Miltenyi Biotec). Polyclonal plasmablasts (PPC) were generated from purified peripheral blood CD19+ B cells in vitro as described (36).
Preparation of cRNA and microarray hybridization
Microarray experiments were performed in Institute of Research in Biotherapy at the Montpellier University Hospital, Montpellier, France (http://irb.chu-montpellier.fr/en/laboratories_microarray.html).
Gene expression profiling analysis
37). We also used the gene expression profiling (GEP) from 72 human normal tissue samples available from Hogenech and coworkers on a public database (38
Real-time RT-PCR
MAGE-C1, MAGE-A3, SSX1, MORC, KM-HN-1, and DDX43 real-time RT-PCR analysis was done as previously described (39GAPDH for each sample, and compared with the values obtained for a positive control (i.e., the testis sample no. 3 used in microarray hybridization) using the following formula in which Ct is cycle threshold: 100/2ΔΔCt, where ΔΔCt = ΔCt unknown − ΔCt positive control.
The expression of CT proteins was assessed by immunocytochemistry on cytospin smears of nine HMCL and of tumor samples from three patients with stage III MM. The following primary Abs were used: the mouse anti-NY-ESO-1 E978 (2.5 μg/ml), the mouse anti-MAGE-A 6C1 (4 μg/ml) mAbs (Zymed Laboratories), the mouse anti-GAGE-7 mAb clone 26 (diluted to 1/250) (BD Biosciences), and the goat polyclonal anti-SSX N-18 Ab (10 μg/ml) (Santa Cruz Biotechnology). Cytospin slides were air-dried at room temperature, fixed in glacial acetone for 10 min, and stored at −20°C until use. The thawed slides were fixed again and endogenous peroxidase activity was quenched with 3% hydrogen peroxide in methanol. The slides were then incubated for 30 min in a blocking solution containing human or goat serum followed by a 15-min incubation in CAS Block™ (Zymed Laboratories). Primary and secondary staining were performed with the NEXES Ventana Medical Systems automaton using the iVIEW diaminobenzidine detection kit (Ventana Medical Systems) containing goat anti-mouse secondary Abs. Primary staining with the goat polyclonal Ab to SSX was performed manually, i.e., a 30-min incubation at room temperature and then the slides were submitted to secondary staining with the automaton using a mouse anti-Goat IgG (Fc-specific) mAb (5 μg/ml; Interchim). Sections of a formalin-fixed, paraffin-embedded normal testis were used as a positive control. Appropriate negative controls omitting the primary Ab were included for each case.
Statistical analysis
37).2 test. The correlations of gene expression signals determined with HG-U133 set arrays and real-time RT-PCR were performed with a Spearman correlation test. The prognostic values of the CT gene expression were analyzed with the Kaplan-Meier method using SPSS software. Hierarchical clustering was performed with the Cluster and TreeView software from Eisen (
We used the list of CT genes available on the CT Gene Database (http://www.cancerimmunity.org/Ctdatabase; Ref. 19) updated with three recently reported CT genes, i.e., CT45 (22), SLLP1/SPACA3 (20), and KM-HN-1 (2119). Furthermore, eight CT genes were not expressed ("absent call") in all samples, including the 10 testes samples. These eight genes were deleted. Among the remaining 46 CT genes with a tissue-restricted expression pattern, all had a "present call" in at least three of five testis samples, but 11 (24%) were not expressed in any of the 64 MMC-samples probed with HG-U133 set arrays. Thus, 35 CT genes, listed in Table I⇓, had a "present call" in at least one of 64 MMC samples. Fig. 1⇓A shows the "present call" frequency curve of the 35 CT genes in MMC. Of note, no CT gene was expressed in all of the 64 MMC samples and 11 CT genes were expressed in <5% of the MMC samples. The expression level of each transcript was also evaluated. Fig. 1⇓B shows the distribution curve of the ratios of the median values of the 35 CT genes in MMC samples with a "present" call to that in the testis. The median of the MMC-to-testis ratios is 0.7 and that of the HMCL-to-testis ratios is 1.2 (see Table I⇓). Fig. 2⇓ shows the HG-U133 set array signal levels in the different groups of cells (testis, MB cells, PPC, BMPC, MGUS, MMC, and HMCL) of the 10 CT genes that were the more frequently expressed in MMC. MAGE-C1 and KM-HN-1 were the two most often expressed genes (66 and 56% of MMC, respectively). MORC, DDX43/HAGE, MAGE-A3, MAGE-A5, MAGE-A6, MAGE-A12, SPACA3, SSX-1, and SSX-4 are expressed in >20% of the patients (Table I⇓). CTAG1B/NY-ESO-1, known to encode for a highly immunogenic Ag, showed the highest median signal intensity relative to testes but were expressed in only 13% of patients with MM. Eleven CT genes were expressed in MMC with a median signal level at least equal to the median expression in testes samples (R = 1). Twenty-seven CT genes were not expressed in plasma cells from patients with MGUS and eight were expressed in at least one of 12 samples: SPANXB, CT45, GAGE1–8, SPACA3, DDX43/HAGE, MORC, MAGE-C1, and KM-HN-1.
Seven CT genes were expressed in some normal B cells, plasmablasts, or normal bone marrow plasma cells, including the following three expressed in >5% of MMC: KM-HN1, SPACA3, and DDX43. Analysis of their expression in the publically available GEP database of 72 normal tissues indicates that KM-HN-1 and SPACA3 are expressed in no normal tissues except testis and thus belong to the tissue-restricted CT category (19). DDX43 is expressed in hemopoietic cells only (i.e., CD14+ and CD33+ cells). Twenty-five of the 35 CT genes are located on the chromosome X (CT-X genes), including 18 genes with a frequency in MMC of >5%. HMCL express CT-X more frequently than primary MMC (median 50 vs 11%; p < 0.001) and with a higher expression level (median ratio 2 vs 0.8; p < 0.001). This is not the case for the 10 non-CT-X genes.
Validation of CT gene microarray expression by real-time RT-PCR
Microarray data for six frequently expressed CT genes were validated by real-time RT-PCR using RNA of purified MMC from 10 patients. Data are shown in Fig. 3⇓ and indicate that real-time RT-PCR data correlated well with microarray signals (p ≤ 0.05).
Validation of CT gene expression by immunochemistry
⇓A⇓A). GAGE-7, MAGE-A, and SSX protein expression were also evidenced on primary MMC from patients (Fig. 4⇓B). Of note, CT protein expression in primary MMC was heterogeneous, because strongly stained MMC close to negative MMC could be observed in the tumor sample of the same patient (Fig. 4⇓B).
Focus on regularly coexpressed CT genes
⇓A shows the percentage of patients' MMC expressing different numbers of CT genes.
Because proteins encoded by CT genes are rarely homogeneously expressed within tumors and to circumvent possible immune-escape mechanisms, it would be advisable to use several different CT Ags in a vaccine design. To focus on a limited set of CT genes, we have explored the combinations of three CT genes, making it possible to cover the MMC expressing at least three CT genes. MAGE-A3, -A6, and -A12 were considered as a single Ag because they encode proteins that are cleaved similarly to generate one common HLA-A2-restricted peptide (cancerimmunity.org/peptidedatabase). The 10 CT genes depicted in Fig. 5⇑B permit us to obtain a combination (actually 15 combinations) of at least three CT genes that are coexpressed in 67% of the patients. The remaining patients expressed one or two genes from this list.
Prognosis value of CT gene expression
⇓). We investigated a correlation of CT gene expression with other conventional prognostic parameters. The MAGE-A3- and MAGE-A6-positive groups contained significantly more patients with a β2-microglobulin level ≥3.5 mg/L (p = 0.04 and p = 0.03, respectively), and the MAGE-A6-positive group contained significantly more patients with a serum albumin level <35 g/L (p = 0.04). Among the CTAG1B- or CTAG2-positive patients, 90% had a chromosome 13 deletion compared with 46% in CTAG1B- or CTAG2-negative patients (p = 0.008). Considering that only the 25 CT genes encoded by chromosome X, a CT-Xhigh⇓A). Patients in the CT-Xhigh cluster had a shorter EFS (median 16 mo) compared with patients in the CT-Xlow cluster (median 32 mo; p = 0.003) (Fig. 7⇓B). Eighty-nine percent of the patients included in the CT-Xhigh group had a stage III disease compared with 71% of the patients in the CT-Xlow group (p = 0.004). Also, the CT-Xhigh group contained 51% of the patients with a β2-microglobulin level ≥ 3.5 mg/L, whereas the CT-Xlow group contained 33% (p = 0.06). Finally, 55% of the patients included in the CT-Xhigh group had a gain of chromosome band 1q21 compared with 27% in the CT-Xlow group (p = 0.008). No differences in hemoglobin level, albumin level, bone lesions, κ L chain isotype, age, C-reactive protein, lactate dehydrogenase, chromosome 13q deletion, chromosome 17p deletion, and t(11;14) or t(4;14) translocations were found between the CT-Xhigh and the CT-Xlow groups.CTAG1B, CTAG2, MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A6 (Fig. 6
One aim of this study was to provide an overview of the expression of known CT genes in primary MMC from newly diagnosed patients. CT genes have previously been classified by Scanlan et al. (19) into four categories according to their expression profile measured by RT-PCR in 13 somatic tissues: 1) testis-restricted (expression in testis and tumor samples only); 2) "tissue restricted" (mRNA detected in two or fewer nongametogenic tissues); 3) "differentially expressed" (mRNA detected in 3–6 nongametogenic tissues); and 4) "ubiquitously expressed". To classify accordingly, we looked for the expression of CT genes among 72 normal (including gametogenic) tissues samples whose GEP were available from Su et al. (38CSAGE2, FTHL17, MAGE-A10, MAGE-C3, SAGE-1, SSX-5, TFDP3, and TSP50), probe sets were likely inefficient because no present call were found in any of the testes samples (10 from Su et al. (38BAGE/CT2, MAGEB2/CT3.2, SYCP1/CT8, NA88A pseudogene/CT18, CTAGE-1/CT21, AF15Q14/CT29, and TPTE/CT44) have previously not been reported as "ubiquitously expressed". We removed these 10 genes from the current analysis. Thus, 46 CT genes answering the criteria defined by Scanlan et al. (19), i.e., testis restricted, tissue restricted, or differentially expressed, were retained.
40). We also confirmed that SPACA3/SLLP1, SPANXB, and the MAGE-A, GAGE, CTAG, and SSX gene families are expressed by MMC (20, 29, 31, 32, 41, 42, 43, 44). The CT Ag CTAG1B/NY-ESO-1, known to be highly immunogenic (27, 45, 46), shows the highest median signal in MMC compared to the testis with a frequency of presence of 13% in MM patients, which is lower than the frequency observed by van Rhee et al. (31).MAGE-C1 is the most frequently expressed CT gene (66%) by MMC of newly diagnosed MM-patients as previously observed in a small group of 29 patients by Dhodapkar et al. (
SPA17 was proposed as a target for immunotherapy in MM (47). However, Scanlan et al. reported it to be expressed ubiquitously (19), and this was confirmed by the present study. In addition, SPA17 was expressed in MMC of 4% of patients with MM only (results not shown).
Ten CT genes whose expression was not presently reported in MMC were identified: KM-HN-1 in 56% of the patients, MORC in 36%, DDX43 in 34%, MAGE-C2 in 13%, PAGE1/GAGE-9 in 11%, XAGE-1 in 11%, SPO11 in 8%, CT45, SPANXC in 6%, and CRISP2 in 5%. For six CT genes—MAGE-C1, KM-HN-1, MAGE-A3, MORC, DDX43, and SSX132, 41). It will be necessary to further extend this protein validation for each of the 35 CT genes expressed in MMC when the specific Abs become available.
The KM-HN-1, SPACA3, MORC, DDX43, and SPO11 genes are also expressed by normal memory B cells, normal plasmablasts, or bone marrow plasma cells. KM-HN-1 could be a B cell differentiation Ag as it is expressed by B or plasma cells only, unlike 72 other normal tissues. Whereas CT genes encoded by chromosome X (except MAGE-C1) are rarely expressed in early disease (zero to one of 11 patients with stage I MM in this study), it is noteworthy that MORC, KM-HN-1, DDX43, and SPACA3 are expressed in MMC from 27 to 36% of the patients with stage I disease. The function of the products of these genes in normal or cancer biology is presently unknown. As vaccination strategies could be of interest for early-stage patients, the identification of these CT genes is of importance and needs further confirmation at the protein level when the Ab will be available. In the same view, KM-HN-1 was also expressed in purified plasma cells from 83% of the patients with MGUS, MAGE-C1 in 33%, DDX43 in 33%, and SPACA3, MORC, GAGE1–8, CT45, and SPANXB in 8%. Patients with MGUS continuously transform into MM with an annual rate of ∼1% (48) and these data may suggest initiating vaccination strategies early in these patients, i.e., when there is still an efficient T cell repertoire against the plasma cell Ags (49).
It is interesting to note that CT gene expression is associated with shorter EFS, significantly in the case of six of the 35 CT genes, all located on chromosome X, and in a "cluster" of patients driven by 25 CT-X genes. No difference in overall survival was found, but the median follow-up of these patients' series was too short to conclude that point. This is in agreement with recent findings in other cancers showing that CT-X gene expression is often coordinated due to hypomethylation of their promoters (50, 51). In addition, CT-X gene expression in patients with epithelial cancers is often associated with a poor patient outcome (52, 53, 54). Our data are also in agreement with recent studies emphasizing that the MMC of patients that express MAGE-C1, MAGE-A3, and/or NY-ESO-1 had an increased proliferative activity and are obtained mainly from patients with stage III MM (32, 40). These gene products might have a functional role in the pathogenesis of cancer leading to a bad evolution. Such a bad prognosis value was recently published for one of the six genes reported here, NY-ESO-1/CTAG1B, in which expression was correlated to abnormal cytogenetics (31). We also found an association between CTAG1B expression and chromosome 13 deletion.
The fact that ultimately all patients with MM relapse, even after HDC and ABSCT, and the concomitant observation that even in the case of a "complete remission" residual MMC remain detectable emphasize the interest in vaccination strategies that might allow the eradication of residual MMC. Vaccination could be initiated directly after HDC to use the "lymphodepletion window" associated with the effect of HDC and the concomitant endogenous production of several cytokines, as reported in a murine melanoma model and in patients with melanoma (55, 56). This strategy is further promoted by the fact that in patients with MM, the efficacy of T cells to pneumococcus Ags was stronger when they were injected 14 days after HDC rather than 3 months (57).
We provide here a strategy for how to choose three CT genes that are frequently coexpressed. To this end, Ag mixtures are envisioned to be superior to single Ag to avoid tumor selection and escape. We show that a combination of at least three Ags could be designed in 67% of the patients using only 10 CT genes. However, additional studies are required before such information could be helpful in the design of vaccination strategies. In particular, the immunogenicity of the gene products of each of these 10 CT genes must be proved, showing either the presence of T cells recognizing antigenic peptides and/or the presence of specific Abs to the gene product in the serum of patients with MM. According to the information available from the CT Gene Database (web site address is given in Results), T cell reactivity has been shown in other cancers for seven of these 10 CT gene products: KM-HN-1, MAGE-C1, MAGEA3/6, SSX4, SPACA3, MAGE-C2, and GAGE1–8. For three of these 10 CT genes, immunogenicity was also shown in patients with MM: a MAGE-C1-specific CD8 T cell detected in patients' bone marrow (58), a MAGE-A3 specific CD8 T cell detected in patients' blood (28), and SPACA3 Abs found in a patient's sera (20). In an allogenic transplantation setting, Atanackovic et al. recently showed that at least four CT Ags expressed by MMC were immunogenic in vivo. Indeed in nine of 35 patients, an allograft resulted in the emergence of anti-SSX2, anti-MAGE-A3, and/or anti-NY-ESO-1 Abs in recipients' sera (59). In the same study, a humoral response against CT Ags was detected in the serum of only one patient of 22 treated with HDC and ABSCT. It is of note that autograft leukapheresis products contain a low number of lymphocytes with an increased proportion of regulatory T cells (60). Given the current data, it is now of major interest to demonstrate that CTL directed against currently identified immunogenic peptides from KM-HN-1, SSX4, SPACA3, MAGE-C2, and GAGE1–8 can be elicited in patients with MM. In addition, we will need to identify immunogenic peptides for MORC, DDX43, and MAGE-A5 gene products for which T cell reactivity is not presently demonstrated. Once this immunological work done, i.e., by associating these CT genes peptides with other tumor-associated Ag peptides reported in MM, e.g., MUC-1 or HM-1.24 (61, 62), one can envision covering almost all patients in a population with a set of 3–5 individually selected immunogenic peptides.
In conclusion, the determination of GEP with pangenomic microarrays could be very useful in defining an optimal combination of patient-specific CT Ags that could overcome tumor escape mechanisms as well as prophylactic vaccination against Ags that will occur in late stage myeloma.
The authors have no financial conflict of interest.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
↵1 This work was supported by grants from the Ligue Nationale Contre Le Cancer (équipe labellisée), Paris, France and from Guillaume Espoir (St. Genis Laval, France). It is part of the national program called "Carte d'Identité des Tumeurs" program (CIT) or Tumor Identity Card developed and funded by the Ligue Nationale Contre le Cancer (http://www.ligue-cancer.net).
↵2 Address correspondence and reprint requests to Dr. Bernard Klein, Institut National de la Santé et de la Recherche Médicale U475, 99 Rue Puech Villa, Montpellier, France. E-mail address: klein{at}montp.inserm.fr
↵3 Abbreviations used in this paper: MM, multiple myeloma; ABSCT, autologous blood stem cell transplantation; BMPC, bone marrow plasma cell; CT, cancer-testis; CT-X, a CT gene located on chromosome X; EFS, event-free survival; GEP, gene expressing profiling; HDC, high-dose chemotherapy; HMCL, human myeloma cell line; MB, peripheral blood memory B cell; MGUS, monoclonal gammopathy of unknown significance; MMC, multiple myeloma cell; PPC, polyclonal plasmablast.
- Received June 15, 2006.
- Accepted December 1, 2006.
- Copyright © 2007 by The American Association of Immunologists


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Tasks Before Apps: Designing Rigorous Learning in a Tech-Rich Classroom
With such a dizzying array of mechanical and virtual help at our disposal, it can be a challenge for educators to know where to start. Educator and technology consultant Monica Burns can relate. Monica wrote this book to share strategies, tools, and insights that teachers can use, regardless of subject or grade level. Monica will teach you to effectively incorporate technology in the classroom focusing on the "three Cs" of technology implementation—creation, curiosity, and collaboration.
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Praise for Tasks Before Apps:
"Monica Burns is one of the leading authorities on the effective use of educational technology. Her latest book does not disappoint, as she compels educators to be more purposeful when it comes to digital tasks. She skillfully leads readers through a thoughtful process that emphasizes a doctrine of pedagogy first, technology second. If you want to get more out of technology, then this book is for you." —Eric Sheninger, Senior Fellow, International Center for Leadership in Education
"Whether you are eager to integrate even more digital tools or are feeling over- whelmed by all the apps and options, Monica Burns is here with practical, classroom- tested advice. In Tasks Before Apps, she wisely keeps the focus on learning goals while showing us how tech tools can scaffold and strengthen students' curiosity, collaboration, and creative potential." —Suzie Boss, author of Reinventing Project-Based Learning, Real-World Projects, and Bringing Innovation to School
Click here to order Tasks Before Apps: Designing Rigorous Learning in a Tech-Rich Classroom
40 Ways to Inject Creativity into Your Classroom with Adobe Spark
Adobe Spark is a fun, free content-creation tool that anyone can use. Easily accessible on web browsers, Chromebooks, and mobile devices, Adobe Spark's intuitive and easy-to-use design makes it the perfect tool for learners of all ages to create . . .
- Video book reports
- Modern, responsive webpage "papers"
- Video journals
- Science posters and videos
- Web newsletters . . . and so much more!
In the hands of creative educators, Adobe Spark provides students with a fun way to embrace critical communication and creativity skills. But what if you don't consider yourself a "creative educator" or just aren't sure where to start?
That's where 40 Ways to Inject Creativity into Your Classroom with Adobe Spark comes to the rescue! Experienced educators Ben Forta and Monica Burns offer step-by-step guidance on how to incorporate this powerful tool into your classroom in ways that are meaningful and relevant. They present 40 fun and practical lesson plans suitable for a variety of ages and subjects as well as 15 graphic organizers to get you started. With the tips, suggestions, and encouragement in this book, you'll find everything you need to inject creativity into your classroom using Adobe Spark.
Praise for 40 Ways to Inject Creativity into Your Classroom with Adobe Spark:
"Adobe Spark is a digital creation game changer and educators Burns and Forta have created the resource to empower any teacher to make students thinking visible and their learning come alive with this simple, yet powerful, tool. 40 Ways to Inject Creativity into your Classroom with Adobe Spark is a clear and practical guide to take student communication,reflection and creation to the next level. Burns and Forta don't give us a 'how to use AdobeSpark,' instead they focus on "how to make student creation the driver of learning." This book is standards-driven keeping the focus on the learning and not on the tool." — Rebecca Hare, Art and Design Educator and Author of The Space: A Guide for Educators
"This comprehensive collection of innovative Adobe Spark projects is a creative shot-in-the- arm for anyone seeking to diversify the digital learning artifacts produced in their classrooms. Ben and Monica provide proper insight and inspiration to get learners of all ages creating and communicating original media content. Their detailed lessons are easy-to-follow and ready-to-use tomorrow." — Andy Leiser, Elementary Integration Specialist, Hastings, Minnesota
Click here to order 40 Ways to Inject Creativity into Your Classroom with Adobe Spark
#FormativeTech: Meaningful, Sustainable, and Scalable Formative Assessment With Technology
The forces of technology and formative assessment combined! Formative assessment is a must for educators, but it can be difficult to juggle with all the demands on a busy teacher's to-do list. #FormativeTech shows how technology tools can energize teacher practice by providing easy ways to implement formative assessment every day. In #FormativeTech, you'll find:
- Practical tips to use #FormativeTech with the whole class, small groups, and individual students
- Ideas for assessing project-based and inquiry-based approached
- How to evaluate data and communicate results with families
In this book you'll find tips to make formative assessment meaningful, sustainable, and scalable.
Praise for #FormativeTech:
"This book is an ideal read for educators looking to grow more agile in their use of data to individualize and personalize instruction." — Dr. Robert Dillon, Director of Innovative Learning
"#FormativeTech is approachable and full of 'use it on Monday' ideas. I can't wait to share this with my colleagues!" — Jennie Magiera, Educator and Author, Courageous Edventures
Click here to order #FormativeTech: Meaningful, Sustainable, and Scalable Formative Assessment With Technology
Taming the Wild Text: Literacy Strategies for Today's Reader
Taming the Wild Text: Literacy Strategies for Today's Reader provides teachers with the best practices for teaching reading to 21st century students. This resource guides teachers in how to meet the changing needs of today's readers while still meeting today's rigorous standards. The big ideas and actionable lessons can be tailored to different grade levels, different types of devices, and different technology plans. Students are reading multiple genres on multiple platforms, and this resource emphasizes integrating technology and finding a balance in the literacy classroom between print and digital texts.
Each chapter is devoted to helping students develop the Five Habits for Reading: reading widely, reading critically, reading deeply, reading closely, and reading purposefully will enable students to tackle texts across all genres and platforms. Each section provides descriptions of key reading skills and strategies that teachers need to introduce and practice in their classrooms, and these strategies are closely tied to today's college and career readiness standards. By providing structures and techniques that support the growing reader, teachers can launch their students into the world of 21st century reading.
Taming the Wild Text: Literacy Strategies for Today's Reader includes:
- 21st Century Reading Tips
- Resource Sidebars
- Checklists
- Graphic Organizers
- Shareable Parent Letters
- Classroom Examples
- EdTech Spotlights
- Technology Tips
Click here to order Taming the Wild Text: Literacy Strategies for Today's Reader
Deeper Learning With QR Codes and Augmented Reality: A Scannable Solution for Your Classroom
Discover scannable technology and how to use it in your classroom, including QR codes and augmented reality. An easy read packed with useful information, examples, and exercises to create engaging and differentiated learning experiences with QR codes and augmented reality, this handbook is a resource for educators and administrators. Through Monica's ACES Framework, teachers learn how to create learning experiences that focus on educational value rather than technological "wow" factor.
What if your students' mobile devices became an instructional asset rather than a distraction? Discover how free, scannable technology can enrich learning, while captivating students. Best of all, these technologies are easy to quickly implement within your classroom.
- Learn about QR codes and Augmented Reality (AR)
- Reach each student with new, hands-on learning opportunities
- Embrace the ACES Framework for teaching with scannable technologies: Access, Curate, Engage, and Share>
- Promote self-directed learning and showcase students' creations
- Leverage technology to connect classroom activities with students' families and the broader community
Praise for Deeper Learning With QR Codes and Augmented Reality:
"This is a book that you will not want to put down. I found the examples and suggestions so exciting that I wanted to try them out immediately. At the same time, I couldn't wait to see what valuable prompts for learning the next page turn would reveal. This is an easy read packed with practical applications." –Debra Las, Science Teacher
"Monica Burns provides educators with simple, practical ideas for integrating QR and AR in the classroom. As AR, QR, and VR continue to play a bigger role in education, this book is a great starting point for teachers who want to integrate engaging, powerful tools and strategies in their classrooms." –Zachary Walker, Professor and Educational Consultant
Click here to order Deeper Learning With QR Codes and Augmented Reality: A Scannable Solution for Your Classroom.
Ready to make this the best year yet?
Sign up for my weekly newsletter and periodic special messages...
and you'll get INSTANT access to my special guide:
12 EdTech Tips for The Most Successful Year Yet!
I can't wait to share my favorite EdTech tips, tools and lesson ideas with you!
Yours in Learning, Monica :)


text:
New Roofing Insulation Product Reduces Material and Labor Costs
-- Flat Roof Insulation -- Tapered Roof Insulation -- Nailbase Roof Insulation -- SIPs for Roof ApplicationsACH EPS roof insulation is recyclable. Any post-industrial scrap or recovered product can be ground and turned into new EPS products or thermally processed into a resin to make other products such as garden furniture, coat hangers and disposable cameras. EPS contains no CFCs, HFCs, HCFCs or formaldehyde. Unlike polyisocyanurate (ISO) and extruded polystyrene (XPS), EPS has no thermal drift and is less costly, making it more affordable to the roofing market.
Contact Information: CONTACT: Sue Rose Rose Public Relations 303.674.3459


text:
Have a laundry problem-free days with Dirt-O, our new natural liquid cleaner!
No more skin irritation caused by residues that detergent leaves behind (SLS, Enzymes, etc) which is one of the triggers for allergies.
Laundry detergent often caused problems such as skin irritation and allergies.
We have developed our own laundry cleaners and softener like no other products is the market.
Dirt-O a gentle laundry cleaner that you can trust.
This product was tried, tested and rated by concerned moms.
Only Beauty Barn provides the safest natural solutions for your family.
On a particular day, our founder was approached by a very despaired father showing his childs skin conditions. It was really heart breaking as the little girl was suffering from very bad dermatitis atopic, and then she decided to provide not just mere skincare solutions, but also household care solutions in order to help this little girl.
Price: Rp250,000
Reviews (0)
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text: How do I take off the back of the phone? Navid Ghomeshi Updated August 18, 2015 18:48 Follow Face the back of the phone towards you On the bottom left corner, pull on the tab. Related articles How do I insert my SIM card? My phone is frozen, what do I do? How do I insert my SD card? How do I improve my battery life? Comments 0 comments Please sign in to leave a comment.


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CBS implement UMS Campus Alert
As part of their endeavour to upgrade safety and security for students and staff, Copenhagen Business School becomes the first Danish university to implement the warning system UMS Campus Alert.
The system, which will be subscription based initially, will allow CBS to send customised SMS-messages and automated calls to fixed-line phones to students and staff in the event of an emergency situation.
The main reason behind the implementation of a warning system is the safety of students and staff, whilst the Danish Ministry for Children, Education and Gender Equality have also stressed that education institutions must have emergency plans and warning systems to support a positive and safe environment.
The story was first published on the website cbsobserver.dk and according to Lars Bergø – CBS' safety inspector – "It is about time that CBS implemented a system that can alert not only employees but also students at CBS".
Learn more about UMS Campus Alert


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"Dear Janelle,
Thank you so much for being part of our special day and also for the beautiful service you gave, I was so relieved everything finally started working out on the day. We were more then happy with the ceremony and how beautiful the day turned out. We will be sure to highly recommend your service to anyone we know intending to marry, we couldn't have asked for a better ceremony and we couldn't have found anyone better to do it then you. So again Thankyou for sharing our day with us and Thankyou for making it so beautiful :)"
"Hi Janelle,
Thankyou for helping us plan the perfect ceremony. Neither of us have been to many weddings and were after something that was uniquely us and that is exactly what you helped us create. You were always there if we had any questions or were after suggestions. The ceremony flowed perfectly and despite some hiccups (a sick bridesmaid who needed to sit down) you continued calmly and professionally after checking she was okay. We could not be happier with our choice in you as a celebrant."
- :) Thankyou Ashleigh and Mitchell - 2015
Thank you for being there for us and making our ceremony so very special. Its no secret that we are a very unorganised couple, your guidance during the lead up to the big day ensured that the day itself was effortless. You were a calming presence during the nerves of the ceremony. I was glad to have you up there with us. And I was grateful for all of your advice, like keeping the bouquet with me. It looks beautiful in the photos. Our ceremony was exactly as we imagined, with some tears, some laughter and a whole lot of love. We have so many very special memories to look back on and thankfully some beautiful photos to help us remember the details."
- Love Mae and Dave - Sept 7th 2014 Xoxox
"Janelle was awesome - so professional, as well as personable, from beginning to end."
- Kimberley - Dec 2014
"She was fabulous with communication and helped tailor a ceremony to both mine and my husbands tastes. She had a handy little book which made it all the more easier as well."
- Ellesha and Michael
"Janelle was great, very caring and very warm. And just made sure that whatever we wanted was in the ceremony."
- Sam - Nov 2014
"Hi Janelle,
We just wanted to say s massive thank you to you for making our ceremony run so smoothly. You spoke beautifully and we felt at ease in your capable hands. Here is a photo one of the guests took, I'm sure Jason's will be better but I thought you might like one of these as well. Have a lovely weekend."
- Rach and Michael Xxxx
"Dear Janelle,
A cheerful hello from us and where has the last year gone? We both have such happy memories of the perfectly simple ceremony you created especially for us. It was just perfect, thank you.
We had always planned on a simple wedding with both Mums, all our siblings, children and grandchildren but unfortunately we didn't consider life's unexpected cruel twists that sadly changed everything. However we both agree the memory you and your family created for us is one we truly treasure.
We both knew that I'd have trouble getting through the vows without a vivid image of Rowan Atkinson as the bumbling minister performing the wedding in 'Love Actually', so we thank you for your patience when I unfortunately laughed and cried while trying to say my vows. We think you are just amazing.
Once again Janelle our deep thanks to you, for making our day so special. "
- J and D xx - Feb 2015
"Dear Janelle,
We had the most amazing day. Thank you for all of your help to making our ceremony personal and special to Luke and I. "
- Mandy and Luke - Oct 2014
"Dear Janelle,
I want to thank you so very much for being the celebrant at our wedding. The whole day went by so quickly that I didn't even get a chance to thank you on the day. You are a professional of the highest standard and a beautiful and caring person and I am so glad that we picked you to be our celebrant. You allowed us the freedom to adapt our own ceremony while still providing us with guidance. You took care of everything so that we had no stress in regards to paperwork and you were ready to meet with us and talk whenever we needed. Thank you so much."
- Lots of love, Mackenzie - April 2014
"Hi Janelle,
Pete and I would like to thank you very much for playing a very important role on our wedding day. Everything flowed beautifullyand the energy in the chapel was just perfect.
Thanks for guiding us and at the same time listening to what it was that we both wanted on our special day. Your professional and friendly manner really allowed us to relax and enjoy every moment of our ceremony. All our guest commented on how lovely the ceremony was and your friendly nature.
Thank you again for being a part of our very special day.
All the very best to you and your family."
- Susie & Pete
"Our special day was made all the more so, by your genuine pleasure and happiness at being our Celebrant.
You have given us such wonderful memories that we will treasure forever, thank you!"
- Nikki & Wes
"Hi Janelle,
We are finally back from cloud nine, it's our first day back at work after our magical 4 weeks off. I just wanted to thank you ... I felt safe in your hands through out the whole wedding ordeal and I thought you did a beautiful job.
I wish I could go back and do it all again!
Take care ..."
- James & Renaee
"Dear Janelle,
Thankyou very much for sharing our special day with is, it meant a lot. Janelle, thankyou for giving us such a special and beautiful service that captured our love perfectly. It will stay in our memories always."
- Love Lance & Jill
"Dear Janelle
We would like to thank you so much for the service you gave us on our special day. Our wedding ceremony at Montville was just want we wanted - romantic, private and relaxed all thanks to you.
We now class you as a close friend of ours seeing we have kept in touch since our wedding day last year. We would recommend you to anyone who would be looking for a celebrant.
Thank you again.
With so much love,"
- Leasa & Dean
"Dear Janelle,
We cannot say enough to show our gratitude for your services on our special day.
Your advice and assistance in the lead up to the wedding eased many of our pre-wedding nerves and we had no doubt in our mind that you were the right celebrant for us.
Your willingness to incorporate two cultures in our ceremony helped is in having the ceremony that we had been talking about for a couple of years.
Your friendly nature had such a great impact on our day that we will remember forever.
Much Love"
- John and Trang
"Dear Janelle,
Thank you so much for making our ceremony all that we dreamed of.
Thank you for your professional and superior service not only leading up to the day but of course on the day and after.
With best wishes and thoughts for good health."
- Kathryn and Lee
"Dear Janelle,
Thankyou so much for your caring and professional service on our wedding day.
Brett and I felt much more relaxed knowing that you had everything under control. You have a lovely manner.
We couldnt have asked for a better celebrant to share our special day.
Best Wishes"
- Love Jane and Brett
"Dear Janelle,
Just a quick not say thank you for being part of Jacks naming day/1st Birthday.
It was great to see you again and as always you did a wonderful job.
- Karen and Mark
"Hi Janelle,
We would like to thank you so much for giving us such a beautiful wedding ceremony. We had many comments about how lovely you were."
- Chloe x
"Hi Janelle,
We just got back from our honeymoon in Tahiti, so beautiful and so lovely to relax after everything!
Thank you so so much for your kind words and helping us to celebrate our marriage. Your calm and relaxed nature helped me to relax and enjoy the ceremony when I was so nervous and on the brink of tears. Couldn't have asked for more!
Once again thank you for making our day so perfect!!"
- Lauren & Darren
We would like to thank you for being our celebrant, but also more than that.. You made the whole process easy and a fantastic experience.
It was a pleasure to have you as a part of our special day and a pleasure to meet you. We will have to catch up next time we are in paradise."
- Love M and V


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We take pride in our Canadian heritage and in the communities where we live, work and play. We're honoured to support and partner with Canada's leading sports teams across the country.
Team Gushue, Official Sponsor of Boost National Grand Slam of Curling
We proudly support Team Gushue at the Boost National Grand Slam of Curling. With the support of brand ambassador Brad Gushue and team, we hope to expand critical community hunger relief causes in Newfoundland and Atlantic Canada. Help support Purolator Tackle Hunger and donate to the Community Food Sharing Association and help us tackle hunger!
Canadian Football League (CFL)
We are a proud long-time sponsor of the CFL, including the CFL Playoffs and Grey Cup since 2003.
We are also a sponsor of each CFL team: the Montreal Alouettes, Ottawa REDBLACKS, Toronto Argonauts, Hamilton Tiger-Cats, Winnipeg Blue Bombers, Saskatchewan Roughriders, Edmonton Eskimos, Calgary Stampeders and the BC Lions.
These sponsorships provide us the foundation to run the award winning Purolator Tackle Hunger® program.


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Bleeding Cool gave you a little rundown of how Doctor Who had been preparing the way for a female incarnation of the character. And indeed, new showrunner Chris Chibnall has stated that this was always going to be the way.
Chibnall said:
"I always knew I wanted the 13th Doctor to be a woman and we're thrilled to have secured our number one choice. Her audition for the Doctor simply blew us all away. Jodie is an in-demand, funny, inspiring, super-smart force of nature and will bring loads of wit, strength and warmth to the role. The 13th Doctor is on her way."
The BBC quotes 35-year old Jodie Whittaker, the 13th Doctor, as saying:
"I'm beyond excited to begin this epic journey – with Chris and with every Whovian on this planet. It's more than an honour to play the Doctor. It means remembering everyone I used to be, while stepping forward to embrace everything the Doctor stands for: hope. I can't wait."
Meanwhile, the incumbent Peter Capaldi said of the new Doctor:
"Anyone who has seen Jodie Whittaker's work will know that she is a wonderful actress of great individuality and charm. She has above all the huge heart to play this most special part. She's going to be a fantastic Doctor."
Here's a look at that reveal moment again…just please, yes, can we avoid this predictable route?


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An easy peasy roast chicken recipe, just throw in all the ingredients…and its ready for roasting!
Rapid Roast Spicy Chicken
1 (3 pound) whole chicken
1 tablespoon olive oil
1/4 teaspoon salt
1/4 teaspoon ground black pepper
1/4 teaspoon dried oregano
1/4 teaspoon dried basil
1/4 teaspoon paprika
1/8 teaspoon cayenne pepper
Preheat oven to 450°F (230°C).
Then, rinse chicken thoroughly inside and out under cold running water and remove all fat. Pat dry with paper towels.
Transfer chicken into a small baking pan. Then rub it with olive oil. In a small bowl, mix the salt, pepper, oregano, basil, paprika and cayenne pepper together. Sprinkle and rub it all over the chicken.
Roast the chicken in the preheated oven for 20 minutes.
Adjust the oven heat to 400°F (205°C) and continue roasting to a minimum internal temperature of 165°F (74°C), about 40 minutes more. Let cool 10 to 15 minutes and serve.
Share and enjoy!
This recipe goes well with:
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Photo by Johanna Reams


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Sankatmochan Mahabali Hanuman 25th January 2017 Written Episode Update
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Sankatmochan Mahabali Hanuman 25th January 2017 Written Episode, Written Update on TellyUpdates.com
The episode starts with ram saying ravana today this war will be over and the decision will be made that religion will win and iniquity will lose. Ram says today I promise if my and hanuman's leg goes back even 1 step I will accept defeat. Ravana laughs and says doesn't matter if you step back but you will die that's for sure and from my hands. Hanuman says the one you are calling a forest liver, your sons and brothers and army died from his and his warriors hands so today you will die from the hands of this forest resident and your body will be at his feet because ego is what will defeat you. ravana laughs and says I will destroy your army and you too monkey and your forest lord. Ravana takes his bow and then prays and says indra dev's agniastra appear. It appears and ravana takes and attacks the agni astra which removes huge storms and rain and lightning from which the enemy will die. Lightning falls and storms come.
Lord ram removes the vayu astra which removes huge storms of wind and tornadoes that destroy the enemy and his attack. The vayu astra removes tornadoes and the attack is tackled and neutralized. Ravana is shocked. Lord ram says today you can fight with iniquity ravana and even tara devi is not here to stop you. Ravana now removes the amrit astra which was made in amrit by devi and it goes toards the army. Ravana says this will destroy the entire army of monkeys and you itself. Lord ram removes chatur astra and attacks the weapon, it stops, ravana laughs and says it wont stop so easily. The weapon starts rotating and ravana says its been made from amrit so it cannot be destroyed. The weapon attacks bolts towards the army of monkeys and they get injured. Everyone is scared and sugreeva says soldiers stay alert and its still attacking us. Lord ram attacks another arrow but nothing happens and the bolts still fall on the army. Laxman says if brother am did not make a promise I would have used my weapons and power, vibhishan says but that weapon has been made from amrit and it cannot be easily destroyed. Hanuman looks in the sky and sees it is moving and thinks ravana has definitely called someone for his help and something is coming and I have to do something. Ravana then attacks a bolt from his powers toards lord ram and the amrit astra goes towards the army, lord ram says hanuman go and protect the army and I am fine here. Hanuman says no lord I cannot leave you alone without a chariot and ravana will again take advantage of the height and injure you. hanuman says lord I feel something is coming from the sky and ravana has called help. Lord ram says don't worry and protect the army and I will take ravana. Hanuman thinks what should I do? And how shall I leave lord ram alone? And I have to protect my army too and then there is something coming from the skies and vibhishan, sugreeva, etc are in trouble. Lord ram says go hanuman. The weapons heads towards the army and bolt towards lord ram. The amrit astra goes and vibhisha holds it for protecting the army.
In heaven, Narayan says everyone in lord ram's army is sacrificing their life and even vibhishan is holding back the amrit astra, vayu dev says but vibhishan cannot hold it back for much long and hanuman has to help them. Indra dev says I have a solution for hanuman's problem.
Hanuman there thinks what should I do? Ravana's bolt is coming toards lord ram and the army, he says this bolt will destroy everyone. Lord ram removes a weapon and then tells hanuman that you have to go hanuman as vibhishan cannot hold it back for much long and don't worry hanuman today I wont let ravana and his power win. Lord ram attacks his arrow and it hits the bolt. The weapon is destroyed. Suddenly a white chariot comes towards lord ram, everyone says what is that? It is the divya rath and everyone say it's the divya rath of lord indra. Hanuman says so lord indra sent his chariot so that I can help the army and you can use the chariot and battle ravana. Ravana thinks I have to teach a lesson to indra dev too and I will destroy this chariot before it is used by lord ram. Ravana removes a bolt. The one who flies the chariot says lord ram, indra dev has sent this chariot for your help but he wont help in the war but his chariot is been offered. Lord ram says I accept the offer. Vibhishan says I cannot hold this weapon any longer and its powerful, vibhishan throws it back, weapons come back and attacks bolts towards the army and more are injured. Lord ram tells hanuman go now and they need your help. Ravana throws the bolt towards the divya rath of lord indra.
Precap: the army of ugradrishtam and his brother is there and vibhishan says this is the army of demon brother ugradrishtam. Ravana laughs and says now this army will kill everyone of you. hanuman throws the amrit astra towards the demon army.
Update Credit to: Tanaya
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text:
4.5kW Pramac
Power COP: 4.5kW 5kVA
Power LPT: 5.4kW 6kVA
Engine: Pramac AP190F
Fuel: Petrol
Phase: 1
Voltage: 230V
Frequency: 50Hz
Controller: Auto Startup
5kW 6kVA Pramac PX8000 Portable Generator
The Pramac UK PX8000 is a compact generator ready to supply power where it's required, its neatly packaged in a strong yet compact frame with integrated handle and wheels as part of the unit, making it easy to transport, the Pramac Super Silent PX8000 petrol portable generator powered by the Pramac AP190F engine is highly-portable, light-weight and compact, making this small generator suitable for plenty of applications such as home, business, power tools, catering and caravaning it's small and with it's integrated wheels has the portability capability to be used anywhere, anytime.
PX8000 Pramac 5kW 6kVA Super Silent Portable Generator
The Pramac PX8000 Diesel Generator efficient, portable and a reliable genset. The ideal small generator for use in residential or business areas without causing a disturbance. As usual from Pramac UK it comes equipped with a reliable petrol engine built with grade A components.
- Pramac (key start-up) engine, AVR
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- Pramac auto start engine, AVR
Pramac PX8000 Portable Generator
The PX8000 Portable Super Silent Diesel Generator fitted with Pramac Engine is perfect for a wide-range of applications;0
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Pramac PX8000 5.4kW Diesel Generator
Call: +44 (0) 1977 657 989 or email: firstname.lastname@example.com for more information
|Pramac AP190F
|27 l
|727 x 515 x 670 mm (L,W,H)
|Electric Starting System
Diesel Generator Help Centre
With so many different diesel generators to choose from, selecting the right set for your requirements can be difficult. With our buyers guide you can confidently select a diesel generator that fit your purpose.
Worldwide delivery can be arranged however it is not included in the price.
If you would like us to supply you with a delivered price then please let us know the final destination of your generator
Please Note: Generators can be picked up from our factory in Yorkshire by yourself or your own choice of transportation, please get in touch to arrange.