text:
Emergency Contact by Mary H.K. Choi
Published by: Simon & Schuster Books for Young Readers
Release Date: March 27, 2018
Genre: YA Contemporary
Format: Paperback
For Penny Lee high school was a total nonevent. Her friends were okay, her grades were fine, and while she somehow managed to land a boyfriend, he doesn't actually know anything about her. When Penny heads to college in Austin, Texas, to learn how to become a writer, it's seventy-nine miles and a zillion light years away from everything she can't wait to leave behind.
Sam's stuck. Literally, figuratively, emotionally, financially. He works at a café and sleeps there too, on a mattress on the floor of an empty storage room upstairs. He knows that this is the god-awful chapter of his life that will serve as inspiration for when he's a famous movie director but right this second the seventeen bucks in his checking account and his dying laptop are really testing him.
When Sam and Penny cross paths it's less meet-cute and more a collision of unbearable awkwardness. Still, they swap numbers and stay in touch—via text—and soon become digitally inseparable, sharing their deepest anxieties and secret dreams without the humiliating weirdness of having to see each other.
I loved this book! It was really adorable and wonderful, and I am still upset that getting it signed at BookCon meant buying a second copy (especially since it's a debut and why would I be getting it signed if I didn't already own the book, but anyways).
First of all, Penny was hilarious! I really loved her and her sass, even if her cynicism probably would annoy me in real life. She's high-key a Slytherin (I'm sorry my brain is all Potter atm) and I absolutely love it. Sam was great too but he mostly made me sad, although not in a bad way. He really needs some love in his life.
I loved all the character development. Penny is a pretty judgemental person at first and Sam is in love with a girl who clearly does not deserve him and plays with his emotions a lot. But they both learn to be more open with other people, which is really nice. Plus their romance was A+ adorable, and they were friends first so no insta-love in sight.
This book also dealt with some much heavier subjects than I expected, even after having read some reviews. But they were very well done and didn't feel heavy-handed, which is the case pretty often. Anyway, I really loved it! Plus we had some Asian-American representation where the mom isn't a tiger mom! Also Penny has the same last name as me, and I really enjoyed that (even though I am 0% Korean).
Overall, this was a very solid contemporary and I'd definitely recommend it. This was a short review but I do not have much more to say other than this: go read it (& support WOC writers)! 🎉🎉
Have you read this? Have you also followed Mary H.K. Choi on everything and think she's the coolest person or is that just me? Let me know!


text:
Dallas Digital Marketing Agency |
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subset: commoncrawl


text: I am working with Jesse on the design of this GG1. He designed the beautiful body, and I designed a motorized chassis that would fit. We still have some work to do. Jesse is working on some scale pantographs, and other details, and I am making a few small improvements to the design.


text:
The Irish American Pub
Welcome Home.
The Irish American Pub
There are thousands of Irish American Pubs all over the world, and in the name of research - the founders of The Irish American Pub have done their best to visit as many as possible to see what makes them so loved. Their goal was to create THE Irish-American Pub.
The Irish American is a recently renovated bar located in the Financial District of Manhattan. With a wide range of draft and bottled beers you are sure to find something to quench your taste buds and enjoy.
Beers ranging from $4-$8, Liquor ranging from $6-$10. The Irish American offers Private Party packages with Sandard, Craft, and Premium drinks. Whether you want to enjoy the conversations at the bar, or sit in one of the many comfortable booths with your friends and loved ones, the Irish American will ensure that you have a great time.
Sláinte! Cheers!


text:
- Architecture Principles, Vision, and Requirements artifacts are intended to capture the surrounding context of for mal architecture models, including general architecture principles, strategic context that forms input for architecture modeling, and requirements generated from the architecture. The architecture context is typically collected in the Preliminary and Architecture Vision phases.
- Business Architecture artifacts capture architectural models of business operation, looking specifically at factors that motivate the enterprise, how the enterprise is organizationally structured, and also what functional capabilities the enterprise has.
- Information Systems Architecture artifacts capture architecture models of IT systems, looking at applications and data in line with the TOGAF ADM phases.
- Technology Architecture artifacts capture procured technology assets that are used to implement and realize information system solutions.
- Architecture Realization artifacts capture change roadmaps showing transition between architecture states and binding statements that are used to steer and govern an implementation of the architecture.
Refer to the following for the detail artifacts association.


text:
Orthopaedic Surgery
His training in spinal Surgery took place at Charing Cross Hospital and The Royal National Orthopaedic Hospital. He also trained in Sweden and Switzerland, with a short time spent in the United States.
He has a wide practice in sports related spinal problems treating many professional and world class sportsmen and women from athletics, swimming, rugby, cricket and professional football, working closely with teams and clubs.


text:
Day by day environment rapidly reacting towards the bad things of human,Trees are most important part of environment ,but bad thing is now a days we are using most of papers ,which are made from trees.So to save a tree many scientific peoples do many research and find out many alternate way for paper making.
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text:
One of the great things about living in London was being able to spend an afternoon snooping around its many fabulous (and famous) markets. One of my favourites was The Stables Market in Camden, which was chock full of the greatest antique and retro home furnishing and collectibles. I loved the fact that you could find the greatest deals on the greatest stuff, allowing you to fashionably kit out your flat without succumbing to exorbitant designer prices. Plus you were also guaranteed to find something completely original, rather than the same-old same-old cookie cutter merchandise.
ReMix Gallery and Warehouse, in West Kelowna, very much fits in with this ethos. It is chock-a-block full of interesting furniture from many decades, and includes some real treasures. In their own words: "At Remix we bring together a fun, curated, and interesting mix of furniture, accessories and art -- a mix that is dynamic and ever-changing… always mixing styles, eras and price-points. What unifies every item is the purpose and passion it was selected with, and the style and personality each item brings to a space."
My Sunday afternoon snoop turned up everything from the gorgeous leather sectional, pictured above, to the perfect 70's hanging basket chair, to a LeCorbusier-style leather loveseat and chair combo, pictured below. A current trawl on their facebook site today has also turned up a vintage Persian rug and a Norwalk ottamon/bench covered in ostrich leather, the latter which would look perfect in my front hall.
The other thing I love about this place is how they display their finds. Each room is a beautifully designed little vignette, so you feel more like you are mooching about in someone's beautifully designed home rather than a shop. If you are willing to do some work, there are also some great fixer-upper pieces to be had. I picked up a cutest 50's-modern bookcase, which as soon as it is repainted - black, white or Schiaparelli pink, I haven't quite decided yet - is going to add the greatest eclectic touch to our spare bedroom.
Remix Warehouse Gallery:
2049 Louie Drive, West Kelowna
Tel: 778-754-3390
Tel: 778-754-3390
Website: www.remixhomestore.com/
Open to the public Sundays from 11am to 4pm.
Open BY APPOINTMENT Mondays to Fridays
(Call 250-870-4280 to book your appointment).
Closed Saturdays.
Open BY APPOINTMENT Mondays to Fridays
(Call 250-870-4280 to book your appointment).
Closed Saturdays.


text:
TVA's Boone Reservoir is located on the South Fork Holston River in northeast Tennessee. The reservoir is named after frontiersman Daniel Boone, who played a major role in the history of the area. Legend holds that he escaped pursuing Native Americans by hiding under a waterfall located on Boone's Creek, a tributary of the Watauga River (which is a tributary to the South Fork Holston River).
In October 2014, a sinkhole was discovered near the base of the earthen embankment at Boone Dam; downhill from the sinkhole, muddy discharge was observed seeping out of the riverbank. TVA engineers are working on stopping the underground seepage caused by flowing groundwater -- project completion is scheduled to be by 2022. The reservoir lake level was lowered and must remain at its current level during the repair to help ensure the safety of downstream residents and workers onsite. For more information about Boone Dam and updates about the repairs visit TVA Dams or the Boone Dam Project webpage, respectively.
The Tennessee Valley Authority is a corporate agency of the United States that provides electricity for business customers and local power distributors serving more than 9 million people in parts of seven southeastern states. For more information about TVA and its mission of service to the Tennessee Valley, click here.


text:
Paulsen Clients Sound Off at NAFB Trade Talk
November 16, 2015
November 16, 2015
The National Association of Farm Broadcasting (NAFB) held its 72nd annual convention in Kansas City, November 11-13.
Trade Talk is a half-day component of the convention that brings farm broadcasters and agribusinesses together for interviews that are broadcast after the convention. Several Paulsen representatives were on hand to assist agency clients in connecting with radio and TV farm broadcasters to conduct interviews.
Paulsen clients participating in Trade Talk included Kubota Tractor Corporation, Exede Satellite Internet and Nutra-Flo fertilizers.
Matt Farr, Marketing Communications Director for Exede Internet expressed his thoughts on the annual Trade Talk event.
"This is our fifth year participating in Trade Talk, and we've found it to be a wonderful opportunity to share the Exede story with farm broadcasters throughout the country," he said. "It's a great way to reach the ag and rural lifestyle audience with information about new products and upgraded services we are now offering."
Marcus Squier, VP, director of client service for Paulsen, and Tori Toering, account specialist with Paulsen, assisted Kubota representatives David Palmer and Kent Brown in sharing new information about Kubota's enhanced M Series tractors and the full line of Kubota Hay Tools.
"Trade Talk is an excellent venue to introduce new products or share information regarding new upgrades on existing models of equipment. It's a very effective and efficient way to connect with the Kubota audience," said Squier.
This was the 15th consecutive year that Paulsen has assisted clients at the Trade Talk event.
Photos (left to right):
Kubota Tractor representatives Dave Palmer, senior product manager, Implements (left) and Kent Brown, senior product manager, Ag Tractors, (right) participated in the NAFB Trade Talk event. Tori Toering, Paulsen account specialist, helped coordinate interviews with numerous farm broadcasters.
Jordin Edwards, Paulsen account specialist, (center) worked with Nutra-Flo representatives Brian Banks (left) and Mark Samuelson (right) to conduct interviews with farm broadcasters during Trade Talk.
Exede Internet representatives Matt Farr, (left) and Alex Miller (right) were joined by Allyse Thaler, Paulsen account specialist, to discuss recent enhancements to the Exede High Speed Internet Service with farm broadcasters from throughout the country.


text:
she rides the green cloud
across the land, a blue heron
casting lines toward astral shoulders
no one told her that those lights
were far away
blue stars,
they look like those wet places
from mother's lore on nested evenings
when hunger was fireless
and her young beak had yet
to show holes
who wants to leave home . . .
but . . . ?
her ankles, no longer deep
in lagoonish brack, her toes
no longer scalded against mud
now full of frogcorpse,
they rise to cloudfloat
on a dare for survival,
refusing at last to eat death
where gills sizzle and fins flip
in the burn of dragonfly bones
in our pond of once-fond memories
that she is leaving in lonely exodus
as twilight paves the sky
toward her only possible future
© J. Celan Smith


text:
Hello. I have a doi number (10.1111/jopr.12650) related to my accepted paper. I want to look my manuscript, but I can't see. When can I see my paper?
Hello Hatice,
Please, reach out to the Wiley Journals teams regarding your manuscript--you can find their contact information here:
Tom with Wiley


text:
New 2019 GMC Sierra 1500 SLT
We'll contact you shortly.
Vehicle Summary
- Condition: New
- Certified: Yes
- Mileage: 7
- Engine: Gas V8 5.3L
- Transmission: 8-Speed Automatic
- Drivetrain: 4WD
- Exterior Color: Onyx Black
- Interior Color:
- City MPG:
- Highway MPG:
Based on EPA mileage ratings. Use for comparison purposes only. Your mileage will vary depending on driving conditions, how you drive and maintain your vehicle, battery-pack age/condition, and other factors.
Vehicle Highlights
Vehicle Details
Max Trailering Package!
New Year - New Ride Sales Event is on now!
Sharper, more capable, intelligent, and innovative, this all new 2019 GMC Sierra 1500 is unlike any truck you know.
This redesigned Next Generation Sierra 1500 stands out against all other light-duty pickup trucks pickups with sharper, more powerful proportions that create a commanding stance on or off the road. Next generation comfort and technology is matched to outstanding performance capability on every trim and configuration. Get ready to live like a pro in the all new 2019 GMC Sierra 1500.
This onyx black Crew Cab 4X4 pickup has an automatic transmission and is powered by a 5.3L V8 16V GDI OHV engine. Given that strapping motor it gets 15.3 L/100 km in the city and 11.2 L/100 km out on the highway according to Transport Canada.
Our Sierra 1500's trim level is SLT. Stepping up to this SLT shows you what GMC means by professional grade. The interior is probably cooler than your office with 8 inch touchscreen, 4G LTE WiFi, Apple CarPlay, Android Auto, SiriusXM, Bluetooth, 4.2 inch customizable Driver Information display, Teen Driver technology, leather seating, heated power front seats, memory settings, dual zone automatic climate control, GMC Connected Access, leather heated steering, remote start and keyless entry, and rear view camera. This luxury is only made better with legendary capability, efficiency, and utility from a ProGrade Trailering system, aluminum wheels, full size spare, cornerstep rear bumper, LED lighting with daytime running lamps fog lights, heated power side mirrors with power folding and auto dimming, GMC MultiPro tailgate with 6 functions designed to help with loading and unloading the cargo box, and EZ lift and lower tailgate function. This vehicle has been upgraded with the following features: Max Trailering Package.
To view and download a brochure open this url https://www.gmccanada.ca/content/dam/gmc/na/ca/en/index/download-a-brochure/02-pdfs/gctr19ct200_2019%20gmc%20sierra%20ld%20catalog_cdn_en_aoda.pdf.
To apply right now for financing use this link : https://gmcldealersecureforms.cdk.com/marshall-en/FinancePreQualForm?originalDomain=http%3A%2F%2Fwww.mightypeacegm.ca%2F&visitorId=340034C00EAD2869F57A75DA7FB8FB81&sessionId=8C4C95B58CDE3B01059E3338A35D8986
Total cash rebate of $6050 is reflected in the price. Credit includes $2650 Delivery Allowance and $3400 Non-Stackable Cash Purchase Assist. Credit is available in lieu of subvented financing rates of 0% for up to 72 months plus $2,650 Delivery Allowance.
Buy this vehicle now for the lowest bi-weekly payment of $394.41 with $0 down for 84 months @ 5.99% APR O.A.C. ( Plus applicable taxes - Plus applicable fees ). Incentives expire 2020-01-31. See dealer for details.
Our dealership is conveniently located at 7501 100th Avenue in Peace River, AB, and we feature a wide array of models to choose from. At Mighty Peace Chevrolet Buick GMC Ltd., not only do we offer a wide selection of new vehicles to choose from, we also provide our customers with a GM Certified service and repair center. Book your test drive with one of our knowledgeable sales staff by calling (780) 624-3681.
Come by and check out our fleet of 30+ used cars and trucks and 70+ new cars and trucks for sale in Peace River. o~o
- Air conditioning, dual-zone automatic climate control
- Assist handles front A-pillar mounted for driver and passenger, rear B-pillar mounted
- Audio system, Premium GMC Infotainment System with 8" diagonal color touch-screen, includes multi-touch display, AM/FM stereo Bluetooth streaming audio for music and most phones; featuring Android Auto and Apple CarPlay capability for compatible phones, advanced voice recognition, in-vehicle apps, personalized profiles for infotainment and vehicle settings
- Brake lining wear indicator
- Cruise control, steering wheel-mounted
- Door locks, power
- Driver memory recalls driver "presets" for power driver seat and outside mirrors
- Floor covering, color-keyed carpeting
- Floor mats, carpeted rear (Deleted when (RIA) All-weather floor liner, LPO is ordered.)
- In-vehicle Trailering App includes checklist, trailer maintenance reminders, trailer security alerts, trailer mileage, tow/haul reminder and trailer electrical diagnostics (Includes trailer tire pressure monitoring alert ONLY when (PTT) trailer tire pressure monitor system is factory ordered and sensors are installed)
- Keyless Open and Start
- Power outlet, box mounted, 120-volt (400 watts shared with (KI4) instrument panel mounted power outlet)
- Power outlet, instrument panel, 120-volt (400 watts shared with (KC9) bed mounted power outlet)
- Radio, HD
- Seat adjuster, driver 10-way power including lumbar
- Seat, Up-level Rear with Storage Package, 60/40 folding bench for Crew Cab models, includes full-length bench seat, seatback storage on left and right side, center fold out armrest with 2 cupholders, full cab width under-seat storage, (includes child seat top tether anchor)
- Seats, front 40/20/40 split-bench with covered armrest storage and under-seat storage (lockable) (Not available with (PCY) SLT Convenience Package, (PDB) SLT Premium Package, (PDT) SLT Premium Plus Package or (PDW) Texas Edition SLT Premium Plus Package.)
- Steering column, lock control, electrical
- Steering wheel audio controls
- Steering wheel, leather-wrapped
- USB Ports (Instrument Panel with bench seat), 2 also includes 1 SD card reader (first row) With bucket seats, USB Ports are located inside console and includes auxiliary jack
- Window, power front, passenger express up/down
- Windows, power rear, express down
- Air vents, rear
- Audio system feature, 6-speaker system
- Bluetooth for phone connectivity to vehicle infotainment system
- Compass, located in instrument cluster
- Defogger, rear-window electric
- Driver Information Center, 4.2" diagonal color display includes driver personalization
- Exterior Temperature Display, located in radio display
- Floor mats, carpeted front (Deleted when (RIA) All-weather floor liner, LPO is ordered.)
- GMC 4G LTE and available built-in Wi-Fi hotspot offers a fast and reliable Internet connection for up to 7 devices; includes data trial for 1 month or 3GB (whichever comes first) (Available Wi-Fi requires compatible mobile device, active OnStar service and data plan. Data plans provided by AT&T. Visit onstar.com for details and system limitations.)
- Instrument cluster, 6-gauge cluster featuring speedometer, fuel level, engine temperature, tachometer, voltage and oil pressure
- Mirror, inside rearview auto-dimming
- Power outlet, front auxiliary, 12-volt
- Power outlet, rear auxiliary, 12-volt
- Remote vehicle starter system
- Seat adjuster, passenger 10-way power including lumbar
- Seating, heated driver and front passenger
- SiriusXM Radio Enjoy a 3-month All Access trial subscription with over 150 channels including commercial-free music, plus sports, news and entertainment. Plus listening on the app and online is included, so you'll hear the best SiriusXM has to offer, anywhere life takes you. Welcome to the world of SiriusXM. (IMPORTANT: The SiriusXM radio trial package is not provided on vehicles that are ordered for Fleet Daily Rental ("FDR") use. If you decide to continue service after your trial, the subscription plan you choose will automatically renew thereafter and you will be charged according to your chosen payment method at then-current rates. Fees and taxes apply. To cancel you must call us at 1-866-635-2349. See our Customer Agreement for complete terms at www.siriusxm.com. All fees and programming subject to change.)
- Steering column, manual Tilt-Wheel and telescoping
- Steering wheel, heated
- Theft-deterrent system, unauthorized entry
- USB ports, dual, charge-only (2nd row)
- Windows, power front, drivers express up/down
- Airbags, dual-stage frontal airbags for driver and front outboard passenger; Seat-mounted side-impact airbags for driver and front outboard passenger; Head-curtain airbags for front and rear outboard seating positions; Includes front outboard Passenger Sensing System for frontal outboard passenger airbag (Always use seat belts and child restraints. Children are safer when properly secured in a rear seat in the appropriate child restraint. See the Owner's Manual for more information.)
- GMC Connected Access with 10 years of standard connectivity which enables services such as, Vehicle Diagnostics, Dealer Maintenance Notification, GMC Smart Driver, Marketplace and more (Limitations apply. Not transferable. Standard connectivity available to original purchaser for ten years from the date of initial vehicle purchase for model year 2018 or newer GMC vehicles. See onstar.com for details and further plan limitations. Connected Access does not include emergency or security services. Availability and additional services enabled by Connected Access are subject to change.)
- Hitch Guidance
- OnStar and GMC connected services capable (Fleet orders receive a 3-month trial. Visit onstar.com for coverage map, details and system limitations. Services vary by model.)
- StabiliTrak, stability control system with Proactive Roll Avoidance and traction control, includes electronic trailer sway control and hill start assist
- Tire Pressure Monitoring System with Tire Fill Alert (does not apply to spare tire)
- Daytime Running Lamps LED signature lighting
- HD Rear Vision Camera
- Hitch Guidance with Hitch View with image adjustment, Pan/Zoom/Tilt
- Rear Seat Reminder
- Teen Driver mode configurable feature that lets you activate customizable vehicle settings associated with a key fob, to encourage safe driving behavior. It can limit certain vehicle features, and it prevents certain safety systems from being turned off. An in-vehicle report card gives you information on driving habits and helps you to continue to coach your new driver
- Alternator, 170 amps (Included and only available with (L84) 5.3L EcoTec3 V8 engine or (L87) 6.2L EcoTec3 V8 engine. Not available with (NHT) Max Trailering Package, (LM2) Duramax 3.0L Turbo-Diesel I6 engine or (VYU) Snow Plow Prep Package.)
- Battery, heavy-duty 730 cold-cranking amps/80 Amp-hr, maintenance-free with rundown protection and retained accessory power (Included and only available with (L84) 5.3L EcoTec3 V8 engine, (L3B) 2.7L Turbo engine and (L87) 6.2L EcoTec3 V8 engine.)
- Capless Fuel Fill
- Cooling, auxiliary external transmission oil cooler
- Differential, heavy-duty locking rear
- Exhaust, single outlet
- Frame, fully-boxed, hydroformed front section
- Pickup bed
- Recovery hooks, front, frame-mounted, Black (Included with 4WD models. Included and only available on 2WD models with (PQB) Driver Alert Package I.)
- Trailer brake controller, integrated
- Transmission, 8-speed automatic, electronically controlled with overdrive and tow/haul mode. Includes Cruise Grade Braking and Powertrain Grade Braking (Included and only available with (L84) 5.3L EcoTec3 V8 engine.)
- Automatic Stop/Start
- Brakes, 4-wheel antilock, 4-wheel disc with DURALIFE rotors
- Cargo tie downs (12), fixed, rated at 500 lbs per corner
- Cooling, external engine oil cooler (Not available with (LM2) Duramax 3.0L Turbo-Diesel I6 engine.)
- Exhaust, aluminized stainless-steel muffler and tailpipe
- Four wheel drive
- GVWR, 7100 lbs. (3221 kg) (Requires Crew Cab 4WD model with (L84) 5.3L EcoTec3 V8 engine or (L87) 6.2L EcoTec3 V8 engine without (NHT) Max Trailering Package. Requires Crew Cab or Double Cab 2WD model with (L84) 5.3L EcoTec3 V8 engine and (NHT) Max Trailering Package. Requires 4WD Double Cab models and (LM2) Duramax 3.0L Turbo-Diesel I6 engine.)
- Rear axle, 3.23 ratio (Not available with (NHT) Max Trailering Package.)
- Steering, Electric Power Steering (EPS) assist, rack-and-pinion
- Transfer case, single speed, electronic Autotrac with push button control (4WD models only)
- Bumper, front chrome lower
- CornerStep, rear bumper
- Fog lamps, LED
- Grille (Chrome surround with high gloss Black mesh and Chrome inserts.)
- Lamps, cargo area, cab mounted integrated with center high mount stop lamp, with switch in bank on left side of steering wheel
- Mirror caps, chrome
- Moldings, window surround, Chrome
- Tailgate, gate function manual with EZ Lift includes power lock and release, includes hitch area light
- Taillamps, LED signature taillight with LED stop and reverse light
- Tire, spare 265/70R17SL all-season, blackwall
- Wheel, 17" x 8" (43.2 cm x 20.3 cm) full-size, steel spare
- Wheels, 18" x 8.5" (45.7 cm x 21.6 cm) 6-spoke machined aluminum with Dark Grey Metallic accents
- Bumper, rear chrome with bumper CornerSteps
- Door handles, chrome
- Glass, deep-tinted
- Headlamps, LED reflector (high intensity) with LED turn signals and LED signature Daytime Running Lamps
- LED Cargo Area Lighting located in cargo box activated with switch on center switch bank or key fob
- Mirrors, outside heated power-adjustable, power-folding and driver-side auto-dimming puddle lamps, side perimeter lighting and memory
- Tailgate and bed rail protection caps, top
- Tailgate, GMC MultiPro Tailgate with six functional load/access features
- Tire carrier lock keyed cylinder lock that utilizes same key as ignition and door
- Tires, 265/65R18SL all-season, blackwall
- Wheelhouse liners, rear
Non-Ontario Residents: Prices do not include additional fees and costs of closing, including government fees and taxes, any finance charges, any dealer documentation fees, any emissions testing fees or other fees. All prices, specifications and availability subject to change without notice. Contact dealer for most current information. Ontario Residents: Prices include freight & PDI, registration, air and tire levies and OMVIC and dealer fees. Prices do not include insurance, license, PPSA and applicable taxes.
Fuel consumption ratings and estimates based on GM testing in accordance with Government of Canada approved test methods. Refer to vehicles.nrcan.gc.ca for details.Your actual fuel consumption may vary.


text:
Furniture units add the much needed value addition and convenience to space. But Furniture fittings are what rightly add value and aesthetic beauty to a piece of furniture.
Furniture Fittings generally includes hardware items such as drawer channels, furniture profiles, furniture hinges, carvings, bed fittings, catchers, shelf supports, wheels, legs, office fittings, key holes, table extension, connecting fittings, and some miscellaneous mix fittings.
Some of the furniture fittings may share the same name as door hardware such as hinges, key holes etc but one should always keep it in mind that these are two separate hardware types for separate products.
Some of the furniture fittings such as furniture profiles, furniture hinges, catchers etc are mainly for the furniture door or cabinet shutter. Some furniture fittings are specific to their use and the product for which they are made such as bed fittings and drawer channels. Furniture fittings such as connecting fittings are generally used for construction of cabinets or furniture. Similarly office furniture fittings are used in offices and study rooms in houses. Table extension furniture fitting helps to save space by increasing the length of the table as and when required.
Furniture fittings help evolve each piece of furniture as a beautifully crafted and created sculpture.
Furniture units add the much needed value addition and convenience to space. But Furniture fittings are what rightly add value and aesthetic beauty to a piece of furniture.
To support this sort of office culture, one can take the help of office fittings. These include cable managers, table extensions, LCD lift ups, drawer organizers, locks etc to name a few.
Legs are generally used to elevate the furniture from the floor to allow for cleaning and also to let the doors of the furniture open and close without hampering or scratching the floor.
Table extension allows increasing the length of the table. A normal table has a fixed size and at times it can become difficult to adjust every person especially during parties and other official meetings.
LCD lifting system is an electrically powered mechanical system which lifts or moves televisions vertically or horizontally out of furniture, ceilings or partition walls.
A bed designed to save space which is otherwise given to a standing bed. This bed gets folded in the wall when not in use. The bed can be folded horizontally or vertically depending on the planning and design of the room.
Drawer Slides - Everything Slides, nothing stay still.
Shelf Support


text:
Original research
Peer reviewed
Effects of two direct-fed microbials on the ability of pigs to resist an infection with Salmonella enterica serovar Typhimurium
Efectos de dos microbianos suministrados directamente en la habilidad de los cerdos para resistir una infección con Salmonella entérica serovar Typhimurium
Effets de l'administration directe de deux préparations bactériennes sur la capacité des porcs à résister à une infection par Salmonella enterica serovar Typhimurium
Mindy J. Spiehs, MS, PhD; Gerald C. Shurson, MS, PhD; Lee J. Johnston, MS, PhD
MJS, GCS: University of Minnesota Department of Animal Science, St Paul, Minnesota. LJJ: University of Minnesota West Central Research and Outreach Center, Morris, Minnesota. Corresponding author: Dr Mindy J. Spiehs, USDA-ARS-NPA US, Meat Animal Research Center, PO Box 166, Clay Center, NE 68933; Tel: 402-762-4271; Fax: 402-762-4273; E-mail: firstname.lastname@example.com.
Cite as: Spiehs MJ, Shurson GC, Johnston LJ. Effects of two direct-fed microbials on the ability of pigs to resist an infection with Salmonella enterica serovar Typhimurium. J Swine Health Prod. 2008;16(1):27–36.
Also available as a PDF.
Objective: To evaluate the effects of direct-fed microbials (DFMs) containing either Bacillus licheniformis and Bacillus subtilis or Enterococcus faecium on the ability of finisher pigs to resist a Salmonella serovar Typhimurium infection.
Materials and methods: Forty finishing pigs were used in a 12-day disease challenge study. Dietary treatments included no DFM (Control; 20 pigs), DFM provided in drinking water (E faecium), or DFM provided in feed (Bacillus). Ten Control pigs were not challenged with Salmonella Typhimurium; the remaining 30 pigs were inoculated on Day 0. Fecal samples were cultured for Salmonella Typhimurium on Days 0 to 5, 9, and 12. Serum haptoglobin (Hp), α1-acid glycoprotein (AGP), IgG, and IgM concentrations were measured. Pigs were euthanized on Day 12, and tissue and digesta were tested for presence and numbers of salmonellae.
Results: Pigs challenged with Salmonella Typhimurium had looser stools (P < .05), greater fecal shedding of salmonellae on Days 2, 3, 4, and 5 (P < .05), and higher serum Hp concentrations on Day 7 (P < .05) than nonchallenged pigs. Serum AGP, IgG, and IgM concentrations were similar between challenged and nonchallenged pigs on all days. Fecal and tissue concentrations of Salmonella Typhimurium and serum Hp, AGP, IgG, and IgM concentrations were similar among challenged groups fed the three dietary treatments.
Implications: Under the conditions of this study, DFMs are not effective in reducing prevalence of Salmonella Typhimurium in feces, gastrointestinal contents, or tissues, or decreasing the number of salmonellae shed.
Objetivo: Evaluar los efectos de los microbianos alimentos directamente (DFMs por sus siglas en inglés) conteniendo o Bacillus licheniformis y Bacillus subtilis o Enterococcus faecium en la habilidad de cerdos de finalización para resistir una infección de Salmonella serovar Typhimurium.
Materiales y métodos: Se utilizaron cuarenta cerdos de finalización en un estudio de enfermedad de 12 días. Los tratamientos de dieta incluyeron no DFM (Control; 20 cerdos), DFM provisto en el agua de bebida (E faecium), o DFM provisto en alimento (Bacillus). Diez cerdos Control no fueron retados con Salmonella Typhimurium; los 30 cerdos restantes fueron inoculados en el Día 0. Se cultivaron muestras fecales en busca de Salmonella Typhimurium los Días 0 a 5, 9, y 12. Se midieron las concentraciones de haptoglobina sérica (Hp por sus siglas en inglés), glicoproteína ácida α1 (AGP por sus siglas en inglés), IgG, e IgM. Los cerdos se sacrificaron el Día 12 y se analizaron tejido y digesta en busca de la presencia y números de salmonellae.
Resultados: Los cerdos retados con Salmonella Typhimurium presentaron el excremento más flojo (P < .05), mayor excreción fecal de salmonellae en los Días 2, 3, 4, y 5 (P < .05), y mayores concentraciones de Hp sérica en el Día 7 (P < .05) que los cerdos no retados. Las concentraciones de AGP, IgG, y IgM séricas fueron similares entre los cerdos retados y los no retados en todos los días de prueba. Las concentraciones fecales y de tejido de Salmonella Typhimurium y de Hp, AGP, IgG, e IgM en suero fueron similares entre los grupos retados que recibieron los diferentes tratamientos.
Implicaciones: Bajo las condiciones de este estudio, los DFMs no son efectivos en la reducción de la prevalencia de Salmonella Typhimurium en heces, contenido gastrointestinal o tejidos ni en la disminución del número de salmonellae excretada.
Objectif: Évaluer les effets de l'administration directe de préparations bactériennes (DFM) contenant soit Bacillus licheniformis et Bacillus subtilis ou Enterococcus faecium sur la capacité de porcs en période de finition à résister à une infection par Salmonella serovar Typhimurium.
Matériel et méthodes: Quarante porcs ont été utilisés pour une infection expérimentale d'une durée de 12 jours. Les traitements alimentaires incluaient aucun DFM (témoin; 20 porcs), DFM dans l'eau de boisson (E faecium), ou DFM dans la nourriture (Bacillus). Dix animaux témoins n'ont pas été inoculés avec Salmonella Typhimurium; les 30 autres animaux ont été inoculés au Jour 0. Des échantillons de fèces ont été cultivés aux Jours 0, 5, 9, et 12 pour vérifier la présence de Salmonella Typhimurium. Les concentrations sériques d'haptoglobine (Hp), de glycoprotéine α1-acide (AGP), d'IgG, et d'IgM ont été mesurées. Les porcs ont été euthanasiés au Jour 12 et des tissus et du contenu intestinal analysés pour détecter la présence et dénombrer les salmonelles.
Résultats: Comparativement aux porcs non-inoculés, les porcs inoculés avec Salmonella Typhimurium avaient des fèces plus molles (P < .05), une excrétion fécale de salmonelles plus importante aux Jours 2, 3, 4, et 5 (P < .05), et des concentrations sériques de Hp plus élevées au Jour 7 (P < .05). Les concentrations sériques d'AGP, d'IgG, et d'IgM étaient similaires pour les animaux inoculés et non-inoculés et ce pour tous les jours de prélèvement. Parmi les groupes inoculés, les concentrations fécales et tissulaires de Salmonella Typhimurium et les concentrations sériques de Hp, d'AGP, d'IgG, et d'IgM étaient similaires pour les animaux des trois traitements alimentaires.
Implications: Dans les conditions expérimentales de la présente étude, les DFM étaient inefficaces à réduire la prévalence de Salmonella Typhimurium dans les fèces, le contenu intestinal, ou les tissus, ou à réduire la quantité de salmonellae excrétée.
Keywords: swine, Salmonella enterica serovar
Typhimurium, direct-fed microbials, food safety, acute phase proteins
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Received: February
13, 2007
Accepted: September 14, 2007
Salmonella is one of the leading causes of foodborne illness in the United States,1 and contaminated pork is a significant source of human salmonellosis.2 Pigs exposed to Salmonella organisms early in life may not appear sick at market age, but can shed salmonellae when stressed and infect other market-aged pigs during transport or lairage.3 Infected pigs can harbor Salmonella organisms in the gastrointestinal tract and lymph nodes.4 Bacteria from tissues and organs of infected pigs have been shown to contaminate the carcass.5,6 Therefore, any significant reduction in prevalence of Salmonella-positive pigs entering the slaughter facility may lessen the risk of carcass contamination.
Production procedures aimed at reducing foodborne pathogens at the farm level may reduce contamination in pork slaughter facilities and throughout the food chain. Dietary manipulation is one pre-harvest management technique that has potential to reduce salmonellae contamination of pork. Altering the diet changes the microbial population in the gastrointestinal tract of the pig7,8 and has been shown to decrease the numbers of salmonellae in the gastrointestinal tract9,10 and feces7,9 of pigs.
Direct-fed microbials (DFMs) are live, naturally occurring microorganisms, fed as supplements, which improve the balance between beneficial and pathogenic microorganisms in the host's intestinal bacterial ecosystem.11,12 Past research on DFMs in swine nutrition focused on growth performance in early-weaned pigs and use of DFMs as alternatives to growth-promoting antimicrobials.13 More recently, DFMs have been evaluated for their potential to improve pig health and food safety through competitive exclusion, immunomodulation, or production of antimicrobials which prevent the fecal shedding and horizontal transmission of Salmonella serovar Typhimurium9,14,15 and Salmonella serovar Choleraesuis.7
Multiple Bacillus and Enterococcus strains may be effective in reducing shedding of salmonellae in swine. Diarrhea was significantly reduced in piglets supplemented with Bacillus cereus,16-18 Bacillus licheniformis,17 or Enterococcus faecium.19 Preventative treatment with E faecium has been shown to prevent Salmonella serovar Pullorum infection in experimentally infected poultry.20 Therefore, a study was conducted to evaluate the effect of DFMs containing either B licheniformis and B subtilis or E faecium strains SF-273 and SF-301 on the ability of finisher pigs to resist an infection with Salmonella Typhimurium, as measured by culture of Salmonella Typhimurium in feces of exposed pigs.
Materials and methods
Experimental design
The experimental protocol used in this study was approved by the University of Minnesota Institutional Animal Care and Use Committee. The study included a 14-day pre-trial period to allow pigs sufficient time to acclimate to diets, and a 12-day disease-challenge study. A total of 40 crossbred, mixed-gender, late-finishing pigs (initial bodyweight = 110 � 0.5 kg) from the University of Minnesota's West Central Research and Outreach Center (WCROC) in Morris, Minnesota, were used in this study.
At the beginning of the 14-day pre-trial period, pigs were weighed individually on an electronic scale accurate to 0.1 kg and allotted randomly to three dietary treatments designated as Control, E faecium, and Bacillus (Table 1).21 Twenty pigs were fed the Control diet, 10 pigs were fed the E faecium diet, and 10 pigs were fed the Bacillus diet. Pigs remained in the wean-to-finish barn for the 14-day pre-trial period.
At the end of the pre-trial period, all 40 pigs were transported to the University of Minnesota Veterinary Medicine isolation facility on the St Paul Campus for the disease-challenge portion of the study. Twelve hours before transport, all feed was removed from the feeders to simulate the commercial practice of withholding feed from slaughter pigs before transport. Immediately after arrival at the isolation facility (Day 0), 30 pigs (10 Control, 10 E faecium, and 10 Bacillus) were inoculated intranasally with a 2-mL suspension (109 colony forming units [CFU] per mL) of a Salmonella Typhimurium strain obtained from the University of Minnesota Veterinary PathoBiology Department. The remaining 10 pigs received a similar inoculation with sterile tryptic soy broth and served as the negative control group (NC).
Room temperature in the isolation facility was recorded once daily between 8:00 am and 10:00 am. Blood samples were collected on Days 0, 7, and 12. Beginning on Day 0, pigs were scored daily for behavior and abdominal distension-gauntness. Fecal scores were determined on samples collected on Days 0, 1, 2, 3, 4, 5, 9, and 12. Daily rectal temperatures were recorded for each pig as a measure of the nonspecific immune response to a Salmonella Typhimurium challenge.
Pigs were euthanized with a barbiturate on Days 12 and 13, and necropsies were performed in the University of Minnesota Veterinary Medicine Diagnostic Laboratory. At necropsy, tonsil, mandibular lymph nodes, liver, spleen, kidney, ileal-cecal lymph nodes, cecum, and a section of the distal ileum were collected aseptically. Additionally, samples of digesta from the ileum and cecum, and fecal samples from the rectum, were collected.
To monitor potential Salmonella contamination of pigs from the feed source, random feed samples were collected from each feeder at the beginning of the pre-trial period and on Days 0 and 12. Samples were pooled by treatment, and 1-g subsamples were cultured for Salmonella at the University of Minnesota St Paul Campus.
Pigs were housed 10 per pen at the WCROC. Treatment groups were maintained separately during transport from the WCROC to the isolation facilities on the St Paul Campus. Upon arrival, pigs were assigned randomly within treatment to 20 isolation pens (two pigs per pen) located in 10 similar, individually ventilated rooms. Each pen provided 1.86 m2 of floor space. Pens were constructed of concrete block and polyvinyl chloride (PVC) planking, and contained solid floors with a center drain in each room. To prevent cross-contamination between pens, the center drain was blocked off, and the space between the floor and PVC planking was sealed completely with water-proof caulk.
Each pen contained one nipple waterer and a two-hole self-feeder. Nipple waterers for pigs given E faecium in the drinking water were attached to two 19-L PVC water bags (The Coleman Company, Wichita, Kansas), with a flow rate of 0.9 L per minute. Bags were filled with water to which E faecium was added at the designated concentration. Water bags were drained and refilled with freshly treated water each morning. Pigs had ad libitum access to water and their respective diets at all times while in the isolation facility.
Dietary treatments
Dietary treatments included a corn-soybean meal diet that contained no DFM (Control); the same corn-soybean meal diet with a DFM given via drinking water (5 � 109 CFU E faecium per pig per day, assuming water consumption of 10 L per day22) (E faecium); and the same corn-soybean meal diet with a DFM included in the feed (1 � 106 CFU of Bacillus organisms per gram of feed) (Bacillus). Probios FS (Chris Hansen Inc, Milwaukee, Wisconsin) was used to provide E faecium SF-273 and SF-301 in the drinking water of pigs on the E faecium treatment at sufficient levels to achieve the manufacturer's recommended dose of 5 � 109 CFU E faecium per pig per day. Inclusion of 0.05% Bioplus 2B (Chris Hansen Inc) in the feed supplied B licheniformis and B subtilis to pigs on the Bacillus treatment. Bioplus 2B, which is approved for use in pelleted diets, was included as per manufacturer's recommendations to achieve 1 � 106 CFU Bacillus organisms per g of feed. All diets were mixed and pelleted at a commercial feed mill and fed in pelleted form.
Table 1: Composition and nutrient content (as-fed) of experimental diets fed to finishing pigs, with direct-fed microbials included either in the prepared feed or in the drinking water
* Premix contained vitamin A, 3,528,000 IU/kg; vitamin D3,661,500 IU/kg; vitamin E, 13,230 IU/kg; vitamin K, 1764 mg/kg; riboflavin, 2646 mg/kg; pantothenic acid, 8820 mg/kg; vitamin B12,17.64 mg/kg; iodine, 441 mg/kg; selenium, 119 mg/kg; zinc, 24,553 mg/kg; iron, 14,553 mg/kg; manganese, 4851 mg/kg; niacin, 15,435 mg/kg; and copper, 1455 mg/kg
† Bioplus 2B (Chris Hansen Inc, Milwaukee, Wisconsin) contains Bacillus licheniformis and Bacillus subtilis and provided a total microbial activity of 6.1 × 105 colony forming units (CFU) (SD, 1.4 × 105 CFU) Bacillus organisms per g of feed. Probios FS (Chris Hansen Inc) contains Enterococcus faecium SF-273 and SF-301. Probios FS was included in the drinking water to provide total microbial activity of 4.2 × 105 CFU/mL (SD, 1.0 × 105 CFU/mL).
‡ Metabolizable energy (ME) was calculated using the method of Noblet and Perez.21
A 20-mL sample of E faecium-treated water from the WCROC and a representative sample of the feed containing Bacillus were collected and submitted to Chris Hansen Inc to test for microbial activity prior to initiation of the study. A sample of E faecium-treated water from the isolation facility was also collected on Day 12 and submitted to Chris Hansen Inc to test for microbial activity. Enterococcus faecium-treated water samples from each facility were tested because the source of the water differed between the two facilities. The source of the Bacillus-treated feed was the same for both facilities.
Diets were formulated to contain equal metabolizable energy (ME), apparent digestible lysine, total calcium, and available phosphorus. All diets were formulated to meet or exceed the National Research Council23 recommendations for ME, total calcium, available phosphorus, and apparent digestible lysine, methionine, threonine, and tryptophan for mixed-gender pigs weighing 100 kg with an expected carcass lean-tissue gain of 300 g per day.
The strain of Salmonella Typhimurium used in this study had been used in previous swine disease-challenge studies to successfully infect nursery pigs, using an oral dose of 2 � 108 CFU per mL.3,23 This strain's resistance to nalidixic acid was used as a marker to distinguish the challenge strain from "wild" strains that may have been present in the environment.
To prepare the inoculation suspension, colonies of Salmonella Typhimurium were grown on XLT-4 agar with nalidixic acid. Ten mL of sterile tryptic soy broth was inoculated with 10 μL of Salmonella Typhimurium and allowed to incubate at 37�C for 24 hours. To determine the number of salmonellae present in the inoculation suspension, subsamples (10 μL) of the inoculation suspension were diluted to 10-5 using sterile peptone water and plated directly on XLT-4 agar containing nalidixic acid (50 μg per mL). Plates were incubated at 37�C for 24 hours, and black Salmonella colonies were counted to estimate the number of salmonellae in the inoculation suspension. The suspension was then diluted using sterile tryptic soy broth to achieve the desired concentration of 109 CFU Salmonella Typhimurium per mL.
Each pig was inoculated with 2 mL of tryptic soy broth suspension. On Day 0, 1 mL of suspension was sprayed into each nostril of each pig receiving the challenge (n = 30). Control pigs received a similar inoculation with sterile tryptic soy broth. Pigs were returned to their respective diets immediately after inoculation. The inoculation suspension was refrigerated overnight. The following day, the inoculation was repeated using only 1 mL tryptic soy broth suspension per pig.
Behavior, abdominal distension, and fecal scoring
Pig behavior and abdominal distension-gauntness was monitored using a subjective scale of 1 to 3. A score of 1 in both categories indicated a healthy, alert pig that appeared to be eating, while a score of 3 in both categories indicated a pig that was not able to stand or appeared severely gaunt. Fecal scores ranged from 1 to 5, with a score of 1 indicating a normal stool and no diarrhea and a score of 5 representing profuse bloody diarrhea. The same person, not blinded to treatment, assessed behavior, abdominal distension-gauntness, and fecal scores each day measurements were recorded.
Serum collection and analyses
Blood from the jugular vein was collected into glass tubes and allowed to clot overnight at a temperature of 4�C. Serum was harvested by centrifugation the following day and assayed for haptoglobin (Hp), α1-acid glycoprotein (AGP), IgM, and IgG concentrations. Serum Hp and AGP were measured via a radial immunodiffusion assay (Cardiotech Services, Louisville, Kentucky). Haptoglobin samples were diluted 1:2 with sterile distilled water before plating, while AGP samples were plated without dilution. Serum IgG concentrations were assayed using a Pig IgG ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, Texas). Serum was diluted 1:100,000 with an assay buffer, and conjugate antibodies used in the ELISA were diluted 1:100,000. Serum IgM concentrations were analyzed using a Pig IgM ELISA Quantitation Kit (Bethyl Laboratories). Serum was diluted 1:100,000 with an assay buffer, and conjugate antibodies used in the ELISA were diluted 1:30,000.
Processing of fecal, feed, tissue, and digesta samples
Fecal samples were collected directly from the rectum of each pig before the initial inoculation on Day 0 and plated on XLT-4 agar plates to ensure that no pig was shedding Salmonella organisms at the onset of the disease challenge. Feed and fecal samples were plated for qualitative and quantitative analysis using 1-g samples. For the qualitative analysis, an enrichment method was used, similar to the procedure used by Isaacson et al24 and Anderson et al,10 except that XLT-4 agar containing nalidixic acid replaced brilliant green agar. Quantitative analysis was accomplished through serial dilutions in sterile peptone water. Samples were diluted to 10-5 and plated directly on XLT-4 agar containing nalidixic acid (50 μg per mL). Plates were incubated at 37�C for 24 hours and black Salmonella colonies were counted to estimate the number of salmonellae shed by each pig. Salmonellae were detectable at a level of ≥ 100 CFU per mL using this method.
Tissue samples were collected aseptically at necropsy, placed in plastic bags containing peptone water, and stored at 4�C for 24 hours before being cultured for Salmonella Typhimurium using a method similar to that described by Hurd et al25 on XLT-4 agar containing naladixic acid (50 μg per mL). Digesta samples from the ileum and cecum and fecal samples from the colon were also stored at 4�C for 24 hours and cultured for Salmonella Typhimurium. Quantitative and qualitative analysis were performed as described for feed and fecal samples, using approximately 1 mL for liquid samples or 1 g for solid samples.
Statistical analysis
All data were collected and analyzed for individual pigs. Results from each pig were then pooled by pen for statistical analysis, with pen the experimental unit. Means of the nonchallenged pens were compared to means of challenged pens (Control, E faecium, and Bacillus) using a Dunnett's contrast to determine the effect of disease challenge. Within challenged pigs, Hp, AGP, IgM, and IgG concentrations, quantitative fecal data, rectal temperature, fecal scores, behavior scores, and abdominal scores were analyzed using a completely randomized design with repeated measures in time using the general linear model procedure of SAS (SAS Institute Inc, Cary, North Carolina). Day post challenge was considered the time factor in the repeated measures analysis. Means separation at each time period was achieved using Fisher's least significant difference of all possible pairwise comparisons. Qualitative analysis of fecal salmonellae shedding and presence of Salmonella Typhimurium in tissue was analyzed using logistic regression (Statistical Program R; Free Software Foundation, Boston, Massachusetts).
A split-plot design was used to analyze the number of salmonellae in tissues and gastrointestinal-tract contents of challenged pigs. Diet served as the whole-plot effect and tissue was the split-plot effect. Whole-plot error was the diet-by-pen interaction. Tissue-by-diet interactions were also evaluated. All means are reported as least squares means. Numbers of salmonellae in feces, tissue, and gastrointestinal-tract contents were analyzed using logarithmic values in order that ANOVA assumptions would not be violated. Least squares means were transformed and reported as actual values; however, pooled standard errors are reported for the logarithmic values.
Effect of disease challenge on clinical signs and mortality
Pigs inoculated with Salmonella Typhimurium developed acute signs of salmonellosis, as measured by higher fecal scores (Table 2) and increased levels of fecal shedding (Table 3) on Days 2, 3, 4, and 5, indicating a successful challenge. As the disease progressed, diarrhea subsided and fecal scores were once again similar between challenged and nonchallenged pigs on Days 9 and 12. Likewise, fecal shedding of Salmonella Typhimurium also decreased by Day 9.
Table 2: Effect of Salmonella Typhimurium challenge and dietary treatment on fecal score from Day 0 to 12*
* Late-finisher pigs were inoculated intranasally with Salmonella Typhimurium on Day 0. Data are least squares means (n = 5 pens/ treatment, 2 pigs/pen). Fecal scores: 1 = firm stool, 2 = semi-solid feces with no blood in stool, 3 = watery diarrhea with no blood in stool, 4 = blood-tinged stool (either loose or formed), 5 = profuse bloody diarrhea.
† NC (negative control): fed control diet, not challenged with Salmonella serovar Typhimurium; Control: fed control diet, challenged with Salmonella Typhimurium; E faecium: fed Control diet, drinking water contained E faecium (approximately 5 × 109 CFU/pig/day), challenged with Salmonella Typhimurium; Bacillus: fed Control diet containing Bacillus organisms (1 × 106 CFU/g/feed), challenged with Salmonella Typhimurium. Dietary treatments were applied from Day -14 to Day 12.
‡ Determined by analysis of variance; P < .05 considered significant.
§ Pre-planned comparison of NC versus challenged pigs (ie, data pooled from the three challenged groups); pooled SEM = 0.47
¶ Pre-planned comparison of Control vs E faecium vs Bacillus;
treatment (P = .02); time (P <
.001); time × treatment (P = .01); pooled
ab Values with no common superscript within a row differ among challenged pigs (P < .05, analysis of variance).
Table 3: Effect of Salmonella Typhimurium challenge and dietary treatment on fecal shedding (CFU/g of feces/day) from Day 0 to 12*
* Pigs were inoculated with Salmonella Typhimurium on Day 0. Data are least squares means (n = 5 pens/treatment, 2 pigs/pen); fecal shedding is the measure of the number of Salmonella serovar Typhimurium in a 1-g or 1-mL fecal sample collected directly from the rectum of the infected pigs. Laboratory techniques were accurate at > 100 colony forming units (CFU) /g of feces; therefore, samples testing positive for Salmonella organisms at < 100 organisms/g of feces are reported as < 100 CFU/g.
† Dietary treatments described in Table 2.
‡ Determined by analysis of variance using logarithmic values; P < .05 considered significant.
§ Pre-planned comparison of NC vs challenged pigs (ie, data pooled from the three challenged groups); pooled SEM = 0.04.
¶ Pre-planned comparison, control vs E faecium vs Bacillus; treatment (P = .72); time (P < .001); time × treatment (P = .53); pooled SEM = 0.05 CFU/g of feces/day.
Seven challenged pigs died suddenly during this study (one Bacillus, two E faecium, and four Control). No nonchallenged pigs died. Four pigs were found dead on the morning of Day 3 and an additional three pigs died on Day 4. All dead pigs were submitted to the University of Minnesota Veterinary Diagnostic Laboratory for postmortem examination. Because the pigs died during a weekend when diagnosticians were not available, postmortem condition of the first four pigs that died was unsuitable for complete examination. Samples were collected from rectum and colon content of all seven pigs. Salmonella Typhimurium was isolated from rectal and colon samples from all pigs at high concentrations (7.9 � 104 to 1.0 � 107 CFU per mL). Additionally, Salmonella Typhimurium was isolated from the lung, intestine, and colon of each of the three pigs that underwent complete postmortem examination. The histopathological diagnosis for all three pigs was septicemia caused by Salmonella Typhimurium.
Despite the high mortality rate of the challenged pigs, abdominal and behavior scores were similar between challenged and nonchallenged pigs (data not shown). Rectal temperatures were also similar between challenged and nonchallenged pigs except on Day 2, when the average temperatures of the challenged and nonchallenged pigs were 40�C and 39.2�C, respectively (P < .05; SEM = 0.01�C).
Serum immunoglobin concentrations and acute phase proteins
Serum IgM (pooled SEM = 0.73 g per L) and IgG (pooled SEM = 2.50 g per L) concentrations were similar between the challenged and nonchallenged pigs. Serum IgM concentrations of the nonchallenged pigs ranged from 8.69 to 11.33 g per L and serum IgM concentrations of the challenged pigs ranged from 9.43 to 12.29 g per L. Serum IgG concentrations were lowest on Day 7 (26.27 g per L) and highest on Day 12 (33.20 g per L) for the nonchallenged pigs. Average serum IgG concentrations for challenged pigs were lowest on Day 0 (29.99 g per L) and highest on Day 12 (41.72 g per L).
Serum Hp concentrations were higher (P < .05; pooled SEM = 0.168 g per L) for challenged pigs (2.64 g per L) than for nonchallenged pigs (1.74 g per L) on Day 7, but were similar on Day 0 (1.06 and 0.91 g per L, respectively, for challenged and nonchallenged pigs) and Day 12 (1.58 and 1.37 g per L, respectively, for challenged and nonchallenged pigs).
Serum AGP concentrations did not differ between the challenged and nonchallenged pigs (P > .05; pooled SEM = 20.0 μg per mL) on Day 0 (300 and 299 μg per mL, respectively), Day 7 (396 and 412 μg per mL, respectively), or Day 12 (330 and 420 μg per mL, respectively).
Serum IgM, IgG, Hp, and AGP concentrations of challenged and nonchallenged pigs increased between Day 0 and Day 12, resulting in a significant time effect (P < .05).
Effect of disease challenge on tissue and digesta cultures
No digesta or tissue samples from the nonchallenged pigs tested positive for Salmonella Typhimurium (data not shown). Overall prevalence of infection in challenged pigs was very high. Additionally, in 100% of challenged pigs, at least one tissue tested positive for Salmonella Typhimurium at necropsy. Among all treatments, tonsils averaged 7.6 � 104 CFU per g Salmonella Typhimurium, which was higher (P < .01) than the concentrations found in the cecum, ileal-cecal lymph nodes, ileum, kidney, liver, mandibular lymph, or spleen of challenged pigs (Table 4).
Table 4: Comparison of number of salmonellae in tissues at 12 days post challenge in pigs inoculated with Salmonella Typhimurium*
* Thirty late-finisher pigs challenged intranasally with Salmonella serovar
Typhimurium, with treatments described in Table 2; treatment (P =
.13); tissue (P < .001); treatment
abcd Values with no common superscript differ significantly (P < .05; analysis of variance performed on logarithmic values).
CFU = colony forming units; LN = lymph node.
Microbial activity of E faecium and Bacillus treatments
The E faecium-treated water sample collected from the WCROC at the beginning of the pre-trial period contained both E faecium SF-273 and E faecium SF-301. Total microbial activity was 4.2 � 1.0 � 105 CFU per mL of drinking water. This was within the acceptable range of microbial activity according to the manufacturer's recommendations. However, the laboratory detected no significant microbial activity in the E faecium-treated water sample collected from the isolation facility on Day 12.
Total microbial activity in the Bacillus-treated feed sample was 6.1 � 1.4 � 105 CFU per g of feed, which adequately achieved the manufacturer's recommendations. Active cultures of B licheniformis and B subtilis were present in the feed sample. Microbial assays confirmed no cross-contamination between the dietary treatments. No detectable levels (ie, > 100 CFU per g) of Salmonella Typhimurium were found in feed samples from any dietary treatment.
Effects of direct-fed microbials on clinical signs and mortality of challenged pigs
Pigs fed the Control diet had higher fecal scores (P < .05) than pigs fed the Bacillus diet on Days 2 and 3, with pigs fed the E faecium diets being intermediate (Table 2). On Day 4, pigs fed diets containing either DFM had firmer stools than pigs fed the Control diet (P < .05). Fecal scores increased immediately after inoculation and returned to pre-inoculation levels by Day 12, resulting in a significant time effect (P < .01). Despite differences in fecal scores, dietary treatment had no effect on the numbers of salmonellae shed in feces of pigs inoculated with Salmonella Typhimurium (Table 3). The numbers of salmonellae shed in the feces increased following inoculation, and then declined by Day 12, resulting in a significant time effect (P < .01).
Average rectal temperatures of all challenged pigs were similar
throughout the study, ranging from a low of 39.1�C on Day 1 to a
high of 40�C on Day 2. There was no effect of dietary treatment on
rectal temperature among the challenged pigs, except on Day 9, when
pigs fed the Control diet had lower mean rectal temperature
(38.9�C) than the pigs fed E faecium (39.7�C) (P <
.05; pooled SEM = 0.02�C). Average temperature of pigs fed the
Bacillus diet was 39.1�C on Day 9, similar to those of pigs
fed the Control and E faecium diets. Throughout the course
of the 12-day study, there was a significant time effect
(P < .01), but no time-by-treatment interaction. Average rectal temperature peaked at Day 2 and returned to normal by Day 4.
Effect of DFM on acute phase protein and immunoglobin concentrations of challenged pigs
Serum concentrations of IgM (pooled SEM = 1.68 g per L) and IgG (pooled SEM = 5.53 g per L) were not affected by dietary treatment. Over the 12-day period, serum IgM concentrations were similar in Control pigs (7.94 to 11.35 g per L), in pigs on the E faecium treatment (9.14 to 13.15 g per L), and in pigs on the Bacillus treatment (10.14 to 12.29 g per L). During the same period, serum IgG concentrations were similar in Control pigs (28.30 to 45.25 g per L), in the E faecium-treated pigs (21.11 to 29.93 g per L), and in the Bacillus-treated pigs (30.50 to 42.65 g per L).
Dietary treatment had no effect on serum Hp concentrations (pooled SEM = 0.30 g per L) or AGP concentrations (pooled SEM = 43.9 μg per L) in pigs challenged with Salmonella Typhimurium. Serum Hp concentrations were similar in pigs fed the Control diet (0.910 to 2.87 g per L), in pigs given the E faecium treatment (1.15 to 2.69 g per L), and in pigs fed the Bacillus treatment (1.11 to 2.43 g per L). Serum AGP concentrations were similar in pigs fed the Control diet (248 to 421 μg per mL), in pigs given the E faecium treatment (301 to 435 μg per mL), and in pigs fed the Bacillus diet (254 to 455 μg per mL).
Serum IgM, IgG, Hp, and AGP concentrations increased between Day
0 and Day 12 in all three challenged treatment groups. This
resulted in a significant time effect
(P < .05) for each blood parameter.
Effect of DFM on tissue and digesta cultures of challenged pigs
Overall concentration of Salmonella Typhimurium in digesta of pigs fed Control (111 CFU per mL), E faecium (182 CFU per mL), and Bacillus diets (161 CFU per mL) were similar (P > .05; SEM = 0.67 CFU per mL). Additionally, there were no differences in concentration of Salmonella Typhimurium among digesta collected from the ileum (< 100 CFU per mL), cecum (551 CFU per mL), or rectum (< 100 CFU per mL) of challenged pigs. Qualitative and quantitative analysis of tissue samples revealed no significant differences among finishing pigs fed the Control, E faecium, and Bacillus dietary treatments (data not shown).
The challenge model used in this study resulted in a Salmonella Typhimurium infection. Higher fecal scores and increased fecal shedding on Days 2, 3, 4, and 5, a febrile response on Day 2, and increased serum Hp concentrations on Day 7 for pigs challenged with Salmonella Typhimurium compared to nonchallenged pigs are evidence of a successful challenge.
The Salmonella Typhimurium infections resulting from our challenge model were more severe than anticipated. Seven challenged pigs died. The most difficult aspect of conducting a disease challenge study is perfecting the challenge model. A previous Salmonella Typhimurium challenge by our research group used oral inoculations of a strain of Salmonella Typhimurium obtained from the Minnesota Department of Health. Challenges using 1 � 106 and 1 � 109 CFU per mL of this organism were unsuccessful, as measured by a lack of salmonellae shed in the feces or any signs of salmonellosis in challenged pigs, eg, elevated body temperature or loose stools.26 Intranasal inoculation elicits more severe clinical disease than intragastric inoculation.27 Previous research28 demonstrated that low and moderate doses of Salmonella Choleraesuis (103 and 106 CFU per mL, respectively) were not sufficient to induce an acute Salmonella Choleraesuis infection in 2- to 8-week-old pigs, but that 109 CFU per mL would achieve clinical salmonellosis Therefore, a dose of 109 CFU per mL was administered via the intranasal route in the current study. A strain of Salmonella Typhimurium that had been previously used for oral inoculations of nursery pigs3,24 was obtained from the Minnesota Department of Health to replace the less virulent strain. The combination of a high challenge dose (109 CFU per mL), intranasal inoculation, and a more virulent strain of Salmonella Typhimurium likely resulted in the more severe disease observed in this study than in other studies using the same strain,3,24 route of administration,27 or challenge dose.28
We expected to see a prolonged febrile response (eg, 3 to 4 days) similar to that noted by other researchers29-32 who inoculated 4- to 5-week-old pigs with intragastric doses of Salmonella Typhimurium ranging from 5 � 108 to 109 CFU. Instead, rectal temperatures were higher for the challenged pigs than for the nonchallenged pigs only on Day 2. However, the pigs in this study were inoculated intranasally. Therefore, acute salmonellosis developed rapidly in these pigs. Because body temperatures were recorded only once daily, and pigs with systemic salmonellosis died suddenly, differences in body temperature and in abdominal and behavior scores were not detected.
The immune system involves two primary types of immunity: innate and acquired. Innate immunity is a nonspecific response that occurs immediately after detection of an immune stimulus. It acts as the first line of defense against pathogens and includes neutrophils, macrophages, natural killer cells, complement, interferons, and acute phase proteins.33 Acquired immunity is a highly specific, inducible humoral and cell-mediated response dependent upon lymphocytes and antibodies.33 In an effort to conduct a comprehensive evaluation of the immune response to Salmonella Typhimurium in the current study, we measured blood parameters that would indicate an innate response (ie, Hp and AGP) and an acquired response (ie, IgM and IgG) of finishing pigs to a challenge with Salmonella Typhimurium.
Serum Hp appears to be the best indicator of immune response in pigs challenged with Salmonella Typhimurium. The increase in serum Hp concentration at 7 days post challenge is consistent with the results of Turner et al,30,31 who also reported a significant increase in serum Hp concentration 7 days after a Salmonella Typhimurium challenge, and a return to postchallenge concentrations 14 days after challenge. Previous studies have demonstrated a positive correlation between immune challenge and serum AGP concentrations.30,31,34,35 However, Neiwold et al36 reported that AGP was only a moderate indicator of immune response in pigs, which may explain why we did not see a difference in serum AGP concentration between challenged and nonchallenged pigs.
Given the higher number of salmonellae shed in feces of challenged pigs than in nonchallenged pigs on Days 2 to 5 and a febrile response in the challenged pigs on Day 2, we expected to see higher serum IgG and IgM concentrations in pigs inoculated with Salmonella Typhimurium than in nonchallenged pigs. Others have demonstrated that serum IgM concentrations increase to a peak level 7 to 21 days following a disease challenge with Salmonella Choleraesuis,27,28 Toxoplasma gondii,37 or Salmonella Typhimurium38 and then begin a steady decline. Therefore, we expected to see a peak in serum IgM concentrations by Day 12. Serum IgG concentrations peak 21 to 49 days after infection with Salmonella Choleraesuis27,28 or Salmonella Typhimurium38 and remain elevated for as long as 3 to 4 weeks after exposure to bacterial pathogens.37 Serum IgG is the most abundant antibody in the body;39 therefore, it was expected that sufficient concentrations of IgG would be present in challenged pigs to detect differences in serum IgG concentrations between challenged and nonchallenged pigs even before the peak levels were reached at 21 days post challenge. Similarly, Turner et al31 also found no response of serum IgG and IgM concentrations to a Salmonella Typhimurium challenge in a 14-day study. They concluded that the enteric infection was most likely contained within the gut via phagocytic cells in the intestinal wall or through an immunoglobulin A-mediated response, resulting in no detectable increase in serum IgG and IgM concentrations. In the current study, it appeared that serum IgG and IgM levels were continuing to increase and may not have peaked by Day 12, which may explain why we were not able to detect differences in serum IgG and IgM concentrations between challenged and nonchallenged pigs.
No tissue or ileal, cecal, or rectal content of nonchallenged pigs tested positive for Salmonella, indicating that biosecurity measures were effective in preventing transfer of the disease from challenged to nonchallenged pigs.
Concentrations of Salmonella organisms in the cecal contents of pigs in this study were higher than the value (< 1 CFU per g) reported at 2 weeks post challenge following an intranasal inoculation with 109 CFU per mL Salmonella Choleraesuis,27 but similar to the concentration in cecal contents (501 CFU per g) of pigs orally infected with 108 Salmonella Cholerasuis.40 Concentration of Salmonella organisms in ileal and rectal contents were not reported in either of these studies.
The overall high number of salmonellae in collected tissues of challenged pigs is consistent with the results of others,4 who found high prevalence of positive cultures from tonsils and from mandibular lymph nodes, which receive lymph drainage from the tonsils. Concentrations of 1.3 � 104 and 5.9 � 104 CFU per g Salmonella Choleraesuis in tonsil have been reported.27,28 High concentrations of Salmonella organisms in cecum, ileum, and ileocecal lymph nodes were also reported.3,41-43 Two weeks after an intranasal inoculation with 109 CFU per mL Salmonella Choleraesuis, Gray et al27 reported tissue concentrations of 2.5 � 105 CFU per g in the ileal-cecal lymph nodes, which was slightly higher than in our study (1.8 � 103 CFU per g). Concentrations of Salmonella Choleraesuis in tonsil ranged from too low to quantify to 1.3 � 104 CFU per g with an intranasal inoculation, but quantitative data was not presented for tissue from the spleen, liver, ileum, or cecum.27 Gitter and Kidd42 conducted necropsies on 4.5- to 7-month-old specific-pathogen-free pigs that were accidentally exposed to Salmonella Typhimurium at 4 weeks of age. Salmonella were isolated most often from the tonsil (10 of 12 pigs) and cecal contents (eight of 12 pigs), with isolation less frequently from small intestinal and rectal contents.
Presence of Salmonella Typhimurium in tissues and the gastrointestinal tract of the challenged pigs has implications for contamination at slaughter. High concentrations of Salmonella Typhimurium in the mesenteric lymph nodes indicate that the organism had spread beyond the gastrointestinal tract, resulting in systemic infection and potential contamination of multiple tissues throughout the body. Mandibular lymph nodes are often incised and inspected for gross lesions by federal meat inspectors at slaughter plants. When lesions are apparent, the lymph nodes are trimmed from the carcass. However, mandibular lymph nodes infected with Salmonella Typhimurium may have a normal appearance;4 therefore, they may not be trimmed from the carcass and could contaminate surfaces throughout the processing line. Preferential persistence of Salmonella Typhimurium in the gastrointestinal tract of swine might result in contamination during evisceration. Surface contamination during evisceration is well documented.5 Bacteria present in the gastrointestinal tract, mouth, and tonsils have been shown to contaminate the carcass.5,6
Dietary treatment had no effect on incidence and level of Salmonella Typhimurium in the challenged pigs, as indicated by similar numbers of salmonellae shed in the feces and similar rectal temperatures. The initial increase in fecal shedding, fecal scores, and rectal temperature, followed by a decline in these parameters at Day 12, are a typical response to an acute Salmonella Typhimurium infection, and the febrile response (eg, 3 to 4 days) is similar to that observed by others.29,32
Previous research evaluating the effects of DFMs on prevalence of Salmonella infection in pigs has produced variable results. Anderson et al7 observed that fewer pigs challenged with Salmonella Choleraesuis and treated with cultures of microbes shed salmonellae in their feces (15% to 18%) than untreated, challenged control pigs (51%), and tonsils were culture-positive in fewer treated pigs (50%) than in controls (83%). Pigs in Anderson's study were inoculated orally with 106 to 107 CFU of Salmonella Choleraesuis. Nisbet et al9 found that pigs challenged orally with 107 CFU of Salmonella Typhimurium and treated with competitive-exclusion microbes were less likely to shed salmonellae in feces (44% ) than those not receiving a treatment (77%), and the cecum was culture-positive for Salmonella Typhimurium in fewer treated pigs (56%) than untreated pigs (100%).9 However, in two studies by Letellier et al,14,15 treatment with a combination of microorganisms in diets did not affect fecal shedding in 12-day old pigs inoculated with 107 CFU of Salmonella Typhimurium.14,15 Only a slightly smaller proportion of carrier pigs, as determined by culture of salmonellae from tissues, was noted.14,15
Viability of the E faecium and Bacillus organisms would influence the ability of the DFMs to effectively compete with Salmonella organisms in the gastrointestinal tracts of the pigs. Lack of viable microorganisms in the E faecium-treated water sample from the isolation facility on Day 12 indicates a possible error in water preparation or laboratory procedures, or decreased viability of bacteria in the water sample, which may have contributed to the lack of response to the E faecium product. The water source for the isolation facility was chlorinated, which may have decreased the viability of the E faecium.44 Viable B licheniformis and B subtilis were present in the Bacillus-treated feed sample, indicating that the heat of pelleting did not alter the viability of the Bacillus organisms. Therefore, it does not appear that a deficiency of viable organisms contributed to the lack of response to the Bacillus product. The overall high numbers of salmonellae in feces, gastrointestinal tract contents, and tissue samples, together with the high mortality rate, indicate a severe disease challenge. Direct-fed microbials must be able to compete with other microorganisms for nutrients and resist inhibition by pathogenic bacteria in the intestine to be effective against an invasion from a pathogen. It is possible that the DFMs were overwhelmed by Salmonella Typhimurium in the gastrointestinal tract. A dose-response study would be necessary to determine the maximum level of Salmonella Typhimurium exposure at which the DFMs would be effective.
Even though fecal shedding of Salmonella Typhimurium was
not affected by dietary treatment, the use of DFMs appeared to
reduce the severity of diarrhea, as indicated by fecal scores. This
may indicate a quicker recovery from the disease challenge for pigs
treated with a DFM. Pigs fed the Bacillus diet had lower
fecal scores than pigs fed the control diet on Days 2 to 4, and
pigs provided with drinking water containing
E faecium had lower fecal scores than control pigs on Day 4. These results are consistent with previous research that has demonstrated less diarrhea when DFMs were fed to weaned pigs.16-19 Pigs supplemented with 107 viable spores of B licheniformis per g of feed had significantly lower diarrhea scores for up to 28 days post weaning than pigs receiving no DFM.17 Others16 reported diarrhea in 36.2% of pigs given no DFM compared to only 18% of pigs receiving a DFM supplement containing 1012 viable spores of B cereus per kg of feed. Incidence of postweaning diarrhea was also lower in pigs supplemented with B cereus (27.4%) than in pigs receiving no DFM supplementation (67.5%)18 and in pigs supplemented with E faecium (21%) than in pigs receiving no DFM supplementation (38%).19
It is not surprising that differences in serum IgM and IgG concentrations were not detected among challenged pigs fed the three dietary treatments, since no differences were detected in serum IgM and IgG concentrations between challenged and nonchallenged pigs. Serum IgM and IgG concentrations of pigs fed the three dietary treatments continued to increase throughout the study, and peaked at Day 12. The lack of a measurable response in serum IgM and IgG concentrations of challenged and unchallenged pigs in this study, the inability to detect differences in IgM and IgG concentrations among challenged pigs, and results reported by other researchers,30 who found no difference in serum IgM and IgG concentrations in a Salmonella Typhimurium challenge model, suggest that serum IgM and IgG concentrations may not be good indicators of clinical Salmonella Typhimurium infection in a 12-day challenge study.
Serum AGP and Hp concentrations of pigs fed the dietary treatments were lowest before challenge, peaked at Day 7, and were beginning to decline by Day 12, which reflects the clinical signs of salmonellosis in our pigs. Fecal shedding and fecal scores also recovered to prechallenge levels by Days 9 to 12. Niewold et al36 found serum Hp concentration to be a good indicator of an acute phase response in pigs. Therefore, it is likely that the pigs in our study were beginning to recover from the acute Salmonella Typhimurium infection by Day 12.
- Under the conditions of this study, treatment with the DFMs Bacillus licheniformis, Bacillus subtilis, and Enterococcus faecium SF-273 and SF-301 does not affect prevalence of Salmonella Typhimurium in feces, gastrointestinal contents, or tissues, or the numbers of organisms cultured from these sites.
- Under the conditions of this study, treatment with DFMs may lessen severity of diarrhea due to Salmonella Typhimurium.
- As tissues of apparently healthy pigs exposed to Salmonella Typhimurium are likely to contain high concentrations of bacteria, care should be taken to prevent exposure of edible lean tissue, equipment, or surfaces at the processing plant to tonsil, mandibular lymph nodes, and gastrointestinal tract contents.
The authors acknowledge financial support of this project by Chris Hansen, Inc.
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13. Nousiainen J, Setala J. Lactic acid bacteria as animal probiotics. In: von Wright S, von Wright A, eds. Lactic Acid Bacteria. New York: Marcel Dekker; 1993:315–356.
14. Letellier A, Messier S, Lessard L, Quessy S. Assessment of different treatments to reduce carriage of Salmonella in swine. Can J Vet Res. 2000; 64:27–31.
*15. Letellier A, Messier S, Lessard L, Quessy S. Host response to different treatments to reduce Salmonella infection in swine. Proc 3rd Int Symp Epidemiol Control Salm Pork. Washington, DC. 1999;317–320.
16. Zani JL, Weykamp da Cruz F, Freitas dos Santos A, Gil-Turnes C. Effect of probiotic CenBiot on control of diarrhea and feed efficiency in pigs. J Appl Microbiol 1998;84:68–71.
17. Kyriakis SC, Tsiloyiannis VK, Vlemmas J, Sarris K, Tsinas AC, Alexopoulos C, Jansegers L. The effect of probiotic LSP 122 on the control of post-weaning diarrhea syndrome in piglets. Res Vet Sci. 1999;67:223–228.
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* Non-refereed references.


text:
The authors explain that we should seriously consider ways of including whole-grain wheat and oat-based recipes in our Christmas holiday meals.
The researchers, from The Rowett Institute of Nutrition and Health, College of Life Sciences and Medicine, University of Aberdeen, Scotland, wanted to test the theory in "a well-designed clinical intervention trial" which points to growing evidence of the benefits of whole-grain foods when looking at the diets and health of various populations worldwide.
This latest study, which included over 200 volunteers, demonstrated that if you ingest three portions of whole-grain foods per day, your risk of high blood pressure is considerably reduced, which in turn lowers your chances of developing diseases linked to hypertension.
Some of the study-participants were given three servings of whole-grain foods each day, in the form of wheat, or both wheat with oats, while the rest received refined cereals and white bread (made of refined flour).
In both the whole-grain and refined-grain groups, the participants were encouraged to eat what they normally ate apart from consuming their apportioned servings.
Study leader, Dr Frank Thies, said:
- "We observed a decrease in systolic blood pressure of 5-6 mm Hg in the volunteers who ate the whole-grain foods, and this effect is similar to that you might expect to get from using blood pressure-lowering drugs.
This drop in systolic blood pressure could potentially decrease the incidence of heart attack and stroke disease by at least 15 and 25% respectively.
It's also good news for the food industry and especially for Scottish food producers."
"Effect of increased consumption of whole-grain foods on blood pressure and other cardiovascular risk markers in healthy middle-aged persons: a randomized controlled trial"
Paula Tighe, Garry Duthie, Nicholas Vaughan, Julie Brittenden, William G Simpson, Susan Duthie, William Mutch, Klaus Wahle, Graham Horgan, and Frank Thies
Am J Clin Nutr vol. 92 no. 4 733-740 doi: 10.3945/ajcn.2010.29417
Written by Christian Nordqvist


text:
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Nil Values
Nil Values
In Lua,
nil is used to represent non-existence or nothingness. It's frequently used to remove a value in a table or destroy a variable in a script. For instance:
In Roblox,
nil can also be used to clear an
Instance object's
Instance/Parent|Parent value and effectively remove it from the game, although it may be possible to bring the object back. Consider this example:
-- Create a new brick local part = Instance.new("Part") -- Parent new part to the workspace, making it viewable part.Parent = workspace wait(1) -- Remove the part from view, but not from memory part.Parent = nil wait(1) -- Part still exists because it's referenced by the variable 'part', so it can be returned to view part.Parent = workspace wait(1) -- Remove the part from view again part.Parent = nil -- Clear part reference so it gets picked up by the garbage collector part = nil


text:
Summer is just around the corner, and you home needs a fresh redecoration!
Summer equals freshness, clean lines and light colors! If you're looking for ideas of how you should redecorate your home, look no more, because we're about to inspire you! So, continue scrolling and see the best lighting fixture for you mid-century environment!
Clean lines, neutral colors, and an amazing dining room set, so you can have the best summer dinners with your family and friends! This set has mid-century furnishing from the mid-century brand, Essential Home, and the lighting fixture couldn't be more well chosen! Vaughan lighting piece belongs to DelightFULL's Heritage Collection, and it's here to enlighten your evenings!
Boho sophisticated dining room décors are very trendy, and the trick is to mix different terracota colors, textures, and, of course, add a golden element to it!
Another great example of how boho chic can rule your house décor! This time, this mid-century retro living room décor is being illuminated by Carter ceiling, a new lighting piece from the portuguese lighting brand, that was presented at iSaloni del Mobile Milano, 2019.
Carter is handmade in brass and aluminum by talented Portuguese artisans that still use ancient techniques to produce these master pieces. Since it's handmade, you can customize the piece, and change the color, finish, size and number of shades.
Summer equals color, and you shouldn't be afraid to be a little bit bold! Don't forget to add a peculiar lighting design, like Cannonball, for example!
Textures, and more textures! Remember that the outside is going to get in your house, sou don't forget to use some plants as décor! This is a way that design uses to make people rethink about their actions towards Nature.
Abbey floor lamp is a mid-century design, handmade in brass and aluminum and it has perforating details on the shades, typical from the fifties! The shades are adjustable, so you can change the direction of the light. This means that this floor lamp is going to be one of your best friends, when you decide to stay in, and enjoy a nice book.
We hope you liked our article! Feel free to pin all the images to your favorite Pinterest board or to print it and use then on your mood board. Don't forget to explore our Twitter and Instagram for more Contemporary Lighting Ideas.
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