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by Ronit
Gupt is a maze escape game, with a twist of puzzle solving. It's a mini game made by a solo developer in Unreal Engine4. Available on itch.io and Gamejolt.
rechronicle 1 year agoGame Graphics Controls
Ronit 1 year agoIf u don't mind can u tell me how far were u able to reach in terms of levels???
rechronicle 1 year agoHey there!
Ronit 1 year agoWell there is a button which slows down the lazer in that level
rechronicle 1 year agoMaybe showing if things are intractable when the player comes near it?
Ronit 1 year agoSay no more
Room To Grow started out as a competition between my friend and I, but escalated into a simple interesting puzzleish game. You start out extremely zoomed in and on level 1, with 0 points and 300 health. Your goal is to get the required score for the level and move on, but along the way you will need to attempt to collect zoomer orbs; purple orbs that zoom out the camera slightly. Currently I update the game ever 2~3 days, and with each updates usually comes a new level.
Shabda is an early prototype of a upcoming game that relies completely on spatial 3d(binaural) sound for navigation. There is a difference between 3d audio and spatial(binaural audio). Binaural audio uses special sounds to trick your brain to position sounds in 3d space as if they were real. With that, you can actually understand how close, far, up or down something is in relation to your head. A first prototype demo is also available with a simple demonstration of game mechanics. In order to run and play it with spatial sound, you need to enable HRTF in your sound system. Please note that without HRTF enabled, it will be close to impossible to play Shabda, as the sounds will just be regular stereo.
RePete is a 2D puzzle platformer about time travel and self-cooperation. You are Pete, a normal guy with abnormal abilities. Loop back in time and help your past self collect gems, solve puzzles, and reach new heights! RePete features four worlds, each introducing new gameplay elements, with over 60 challenging levels to test your ability to work with yourself. The demo version features the first two worlds so you can try before you buy!
Need feedback about demo.One_helluva_day - DemoSelf-centered CEO trying to save his company from bankruptcy forced to rescuing whole town from impending disaster. Funny, old school cartoon point and click adventure in the style of "Lucas Arts" and In the tradition of "The day of tentacle". Inspired by games like "Sam and max hit the road" and "Secret of the monkey island". The game is filled, a little crazy tasks. Some of them can be solved in different ways. The plot tells about the - One unfortunate day of the life ordinary directors of a small firm, full of mysticism, conspiracies, cults and the end of the world. Funny and crazy and twisted storyline will make you remember the point and click adventure in which you play as a child. During the passage you will meet fun and interesting characters who amuse you and hint how to get some of the tasks.


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Once a sleepy village, Selimiye has become a place to be seen, with an up market feel attracting well heeled Turks from far afield. It's kept it's charm though and with a good choice of restaurants and several mini markets one wonders how it ever coped before the quay was extended.
Selimiye has also welcomed home one of it's sons, the famous Osman with a mouth full of gold teeth who previously ran a restaurant in Bozburun. No-one really knows why he's famous (possibly himself included) but he keeps telling everyone he is! His current restaurant is near the quay and he's the sort who will happily help you out with anything else you need.
It's a far cry from when I first visited when even sourcing a loaf of bread was a challenge – the local bakery will now be happy to sort that for you. Showers are available near the quay and there's an ATM too on the edge of the village.

subset: commoncrawl


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Over the last few months, we have been preparing a social media strategy for N2Africa. The launch of this strategy will coincide with the November meeting, with a dedicated Facebook page as a central element. The Facebook page will promote N2Africa activity and link directly with partners and supporters, some of whom already have a social media presence. The N2Africa Facebook page will be updated regularly with videos, pictures and articles sourced from the Podcaster. If you have suggestions about what to feature, please let us know.
The N2Africa website will also be updated with a new interactive map. The map will pin videos and photos to project locations and is a means to demonstrate the geographic extent of N2Africa, as well as to illustrate and share our D2R successes and challenges. To begin with, the map will contain a select number of videos for each country, but will grow to include more videos as well as a library of photographs. Videos played in the map will be linked from new N2Africa 'channel', hosted on Vimeo. This dedicated video channel will host the N2Africa video archive of longer films and training materials.
The Facebook page, map and Vimeo channel will provide an extension to the Podcaster, to link, enhance and celebrate the work of a growing a federation of development and research partners. We look forward to connecting with you soon through Facebook.
Alastair Simmons


text: An anti-terrorism court (ATC) of Lahore on Wednesday extended interim bail of Hassan Niazi along with ten lawyers in Punjab Institute of Cardiology (PIC) attack case, till January 20. The hearing of the case was conducted by ATC judge Arshad Hussain Bhutta. The investigation officer failed to present Photographic test report in today's hearing. The hearing court extended interim bail of Prime Minister Imran Khan's nephew Hassan Niazi along with 10 lawyers till January 20.At the previous hearing of the case, the judge had extended Hassan Niazi and nine other lawyers' bail until Jan 15 (today) and directed all the accused to appear before the investigation officer for their photographic test. The investigation officer had told the court that a forensic lab has been provided with video clips and images of the lawyer's hooliganism outside the PIC for the test.A group of more than 200 lawyers, who had an ongoing 'tussle' with the doctors of the PIC, had stormed the hospital , vandalised property and damaged dozens of vehicles. They also set ablaze a police van during their assault. At least 52 lawyers were arrested after the attack and were presented before the court.Two FIRs were registered against over 250 lawyers who attacked Punjab Institute of Cardiology (PIC) in Lahore.


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Estuaries – which are the most seaward part of rivers under the influence of tides - are often strongly human managed. Their geographic location makes them prime candidates for harbor infrastructure, and large populations nearby need to be protected against flooding. Hence, management often encompasses deepening of the waterway to allow ship travel, and construction of dikes and/or a storm surge barrier to prevent flooding calamities. At the same time, estuaries provide unique ecosystems that deliver valuable ecosystem services to society.
The long-term, large scale character of human management interventions causes a need to assess the impacts on the natural environment. In a new study, Stijn Temmerman and Patrick Meire (Global Change Ecology Centre, Research Group Ecosystem Management), together with their Dutch and Belgian colleagues, predict the long-term impact of human management practices on the natural environment in the Westerschelde and the Oosterschelde. They specifically focused on macrozoobenthic organisms. These are tiny animals, such as shellfish and worms that live on and within the sediment of estuaries.
The common cockle (Cerastoderma edule) and the Annelid worm Heteromastus filiformis (picture by Hans Hillewaert) are two examples of benthic estuarine species.
The Oosterschelde and Westerschelde are particularly interesting, as they represent case-studies for the effects of two large scale management options: a storm surge barrier in the Oosterschelde, and strong deepening of the channel for shipping purposes in the Westerschelde.
Patrick Meire: "We integrated empirical and modelling data on landscape morphology, hydrodynamics and benthic organisms' ecology. Our analysis clearly shows the long-term implications of both forms of estuarine engineering. Deepening of the channel in the Westerschelde lead to an increase of tidal dynamics and stronger variations in salinity, which induces stress on the benthic community. In the Oosterschelde, the effect of the storm surge barrier was much more mixed. The Oosterschelde was transformed into a shallow, marine embayment. This actually induced an unexpected improvement of the environmental quality. The Oosterschelde now has a flourishing benthic community."
These results clearly show the importance of long-term assessments of large human interventions in estuaries. Before the implementation of both the storm surge barrier and the deepening, the Oosterschelde and Westerschelde had similar current velocities and tidal dynamics. Within 50 years, both systems now have an incomparable ecological functioning.
Stijn Temmerman: "Our results clearly show that we should not only focus on direct, short-term impact assessments. We should put much more emphasis on the long-term effects on the natural environment. The divergent human pressures on the Oosterschelde and Westerschelde could happen in the near future for many estuaries worldwide. Therefore our analysis is a valuable source of information for coastal managers around the world".
The results were published in the scientific journal Estuarine, Coastal and Shelf Science.
Aerial picture of the Oosterschelde and the Westerschelde. The storm surge barrier on the Oosterschelde is clearly visible, as is the port of Antwerp adjacent to the Westerschelde. Source: beeldbank.rws.nl, Rijkswaterstaat


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Note: Translated words like 'Heart' indicate unusual relationships between the character positions in the word(s) being translated and result of the translation.
God (UFO/ET) talks to us through the ET Corn Gods language translation of words.
H is 8+66 = 74, 7od, "GOD".
e is 5, book Dt = "ET". (d us 500, 5, "E")
e is five = i-f ve = "Cve".
v is v+66+66 … = "od".
Heart = "GOD ET (Code) Art".
Note: The Crop Circle formation consists of lots of Bars (Play, not serious). See: [HERE]
Visit: www.ETCornGods.com


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Barbados Is One Of The Caribbean Islands-Barbados Villa Rentals
Best time to visit Barbados
It is always a wonderful time to visit Barbados. This is because the weather is always spectacular throughout the year. January is a good month for a holiday. However, July is the busiest month in Barbados. The tourist season is from mid-December to mid-April. And so it is expected that housing will be more expensive even during this time.
On the other hand, hurricane season is from June to October and the highest risk of storm is from September to October. During this time you should take care of the weather while traveling.
Places to visit
Bathereba is the main destination for surfers. If you want to feel isolated, this is also the area. In Bathsheba you can enjoy long walks on the beaches. But you can also enjoy the mountains behind a long stretch of beach. At night, you will also enjoy the atmosphere as you only feel peace and quiet with a crowd of frogs and fireflies at night sharing your company.
To explore the city, you must visit the only city in Barbados which is Bridgetown. You can explore the surroundings of the city and the rum shops. While doing so you can also take a break to grab snacks and enjoy the constitution river café. You can also do some shopping. Just head to Broad St. and you will never be disappointed. You can also enjoy local culture at Swan St.
In Central Barbados, sightseeing is sports. Whether you want to see the natural beauty of Barbados or take a historical trip around the area, this is the place. The rolling hills would be a pleasure to explore. And the lush scenery can be breathtaking as well.
Eastern Barbados is truly a haven for surfers. With the Atlantic waters and strong winds, the beautiful vistas are quite fun to be on the bus. You will enjoy the never ending waves that also make the atmosphere and rugged beaches look attractive. That's why tourists fall in love with the place. And people actually keep coming back too.
The southern coast is where development is taking place. The long journey from the airport to Bridget is proof of this. Nevertheless, this is also the place where you will find most of the accommodation ranging from cheap to almost mid price. Away from the main road, you will also find the strip of Dover Beach and St Lawrence Gap quite attractive. If you want some isolation or surfing adventure, you can also visit the area of Silver Sand.
Southeast Barbados is also a bit quieter. But this is where you can find small resorts and small villages scattered all over the place. Enjoy the beautiful beaches in the region as you would in other parts of Barbados.
The west coast is where the rich keep their holiday homes. Although most beaches are public. Development is also taking place here, so you can expect the beaches to be hidden due to the structures built in this area.
All the things you can do in barbados
Like the rest of the Caribbean islands, visiting Barbados also means being surrounded by beaches. And so, people usually go to Barbados to enjoy the white sand and pristine beaches. However, with the influx of tourists in the region, a lot of development is happening every now and then. Because of this, you can also enjoy resorts and bars all over the place. When the mood suits you, you can either have some refreshments or have some coffee. Food will also be on demand for your palate. With hotels and resorts built there, a wide variety of cuisines are definitely available.
Of course, you should not forget the lush sceneries and rolling hills in Barbados as well. Because of this, you can also expect to find wildlife in the area. Such adventurers are also trying to complete their Barbados adventure.
Barbados Holiday Rentals Barbados Villa Rentals St. James La Paloma
Hotels eliminate Barbados. Vacation rentals such as Holiday Villas and Holiday Cottages are also present there. Whether you want to stay in an attractive and private holiday accommodation or in attractive resorts, you will definitely find it in Barbados. Affordable housing is available for groups and families. Wherever you live, you will still be able to explore and enjoy your entire Barbados vacation. All in all, whether you want to swim in the pristine beaches of Barbados or explore the island, it will always be best to have a time of relationships and adventures with family or friends.
Places of interest in Barbados
Graeme Hall Nature Sanctuary
Harrison's cave
Animal flower cave
Barbados Wildlife Reserve
Morgan Lewis Windmill
Queen's College (Barbados)
Andromeda Gardens
National heroes class
Francia Great House
Flower forest
Hey I'm Jai William. I started Villa Marketers in 2005 in light of the fact that I realized that there were many razed house owners like you, who had a terrible property, yet the appointments could not be measured to qualify.
- Mysore Tourist Places, Places To Visit In Mysore In One Day
- This Post All Image Cover Image Credit: Pixabay


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January 2013 Update
- Details
- Category: Background
- Published on Tuesday, 15 January 2013 13:05
- Written by Super User
- Hits: 2719
Update on Project
So far I have purchased several pieces of electronics and have a vague plan for the flight system. The raspberry pi has 2xUSB ports and a GPIO. The GPIO has supports one UART, one I2C and two SPI. For the flight system I require the ability to control servos and have knowledge of the helicopters orientation and how fast it is moving, accelerating. The helicopter also needs to be able to detect obstacles.
The address these I am going to develop three systems as detailed below.
Orientation & Position System
To detect the helicopters position I have decided to have a suit of 4 sensors. These are:
GPS (connected via USB initally)
Compass 3-D connected via I2C – Honeywell HMC5883 Module Three-Axis Digital Compass HMC5883L S...
Accelerometer 3-D connected via SPI Analog Devices ADXL345
Pressure Gauge connected via SPI on ATmega328 microcontroller. Note not sure how much one will be able to read form the pressure, considering pressure varies with weather and typically changes from 101.325kPa to 100.748kPa in 40 meters of altitude at around sea level. The sensor I have purchased is a MS5611 high-resolution atmospheric pressure module height sensor IIC SPI 24-bit
Obstacle Detection
Initially connected via the obstacles will be done solely using 6 (HC-SR04 range 4.5m) ultrasonic sensors measuring the distance to the obstacles. One will be mounted underneath to see if the helicopter has landed. The ultrasonic sensors with be connected to an Atmega328 microcontroller which will then be attached to the UART GPIO port on the raspberry pi. The Atmega328 SPI port will have the pressure sensor attached.
Flight Control System
The flight control system will adjust upto 7 servos that will control the blades and control surfaces of the helicopter. The 7 Servo Controller will be connected via one of the SPI ports.
SPI breakout board to control 7 servo motors,for AVR, arduino, PIC, Raspberry Pi Bitwizard.nl
Before tiring to run (fly) before I can walk (drive), I will start mount the system on a test buggy. The buggy I am going to use is an old toy I had and built as a kid. The buggy currently has some damage and needs a good clean and a bit of soldiering tlc etc. But should prove a good test platform before flying.


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Fairfield, Conn. - Dr. Mark Schenker will visit to discuss the 2020 One Book One Town (OBOT) selection, Running with Sherman: The Donkey with the Heart of a Hero by Christopher McDougall, on Tuesday, March 3 at 7:00 pm at the Fairfield Public Library, 1080 Old Post Road. This lively analysis and discussion is always a favorite OBOT event. Dr. Schenker has led book and film discussions in Connecticut for more than 30 years and has given numerous lectures on writers and their works. Many of those lectures have been here at the Fairfield Public Library where he is one of our most popular presenters. Join us and share your perspective, too. Registration is requested.
Dr. Mark Schenker is a Senior Associate Dean of Yale College and Dean of Academic Affairs.
If you haven't already, start reading Running with Sherman, the story of a rescue donkey, his owner and the friends they make along the way. For more details about One Book One Town events including book discussions, lectures and the author visit, please visit the Library's website at fairfieldpubliclibrary.org/OBOT/.
This is the twelfth One Book One Town celebration in Fairfield. One Book One Town is made possible through the sponsorship of the Friends of the Fairfield Public Library, The Junior Women's Club of Fairfield, the Fairfield Public Library, the Pequot Library and Fairfield University.
All programs at the Fairfield Public Library are free. To register, or for more information, visit us online at: fairfieldpubliclibrary.org or call 203-256-3160. Follow the Fairfield Public Library on Twitter @fairfieldpublib and Facebook @fairfieldlibrary.


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Car Buying Guide for eBay Motors Scams
[amazon_link asins='0596101783′ template='ProductLink' store='retite-20′ marketplace='US' link_id='0918754f-4b73-11e8-bb40-db9c7a2551ec']When buying a used car you'll need to find a good Car Buying Guide that prevents you from making mistakes. Many people in the past few years have been scammed when buying cars online from sites like eBay Motors. Phishing and Wire Fraud scams plague eBay Motors daily to prey on unsuspecting new buyers that promise such things as Vehicle Purchase Protection, and buyer protection when making payments using PayPal but really there is no protection at all. Buyers shouldn't shop on eBay Motors for vehicles because scammers will list "too good to be true" exotic or popular vehicles at low prices to lour or prey on multiple bidders by offering "Last Chance" opportunities or offers to hold the vehicle if a Bank Wire transfer is made within a cretin amount of time. The seller may also phish your information to create or obtain access to your eBay account to list more vehicles. Don't fall victim to these scams!
- eBay Motors offers no protection for your vehicle or money.
- PayPal will refund deposits on titled property giving buyers the vehicle and money back without investigation.
- Don't be mislead by eBay Motors sellers, take your business to legit sites like AutoTrader.
- Google "eBay Motors Sucks" for 90 days of Phishing Scams reported by the Car Doctor.
- Scammers try to get you to send them money in advance for whatever reason.
CrowdHunters gets Scammed on eBay Motors
[amazon_link asins='0596101783,0143038885,0973532831′ template='ProductCarousel' store='retite-20′ marketplace='US' link_id='64cb575e-4b73-11e8-89ad-6dad0e58fa32′]
eBay Motors Abusive Seller Hubcapjoes
eBay Motors has been touted by their number one fanboy Hubcapjoes as being one of the best places to shop online for vehicles and automotive parts but if you look into the past of this fanboy you'll see that he is nothing more than a shady promoter working for eBay Inc as a paid spokesman. Hubcapjoes, and other eBay Inc employees use YouTube to promote new buyers to the site so they can be scammed. We recommend that if you're selling your vehicles online please use an honest site like AutoTrader or do the deal yourself from Craigslist. You'll save yourself a world of hurt to avoid car buying fraud on eBay.
eBay Activist Protects You – Car Buying Guide
In the following video below Meet the Car Doctor Ed Koon, he's an eBay Motors Activists that has been protecting consumers from eBay fraud for nearly 15 years online. His website shows people how to buy used cars and gives them advice on how to spot fraudulent listings. In this video he captures eBay Inc spying on his auto-blog and trying to plant cookies on his computer in order to SQL inject his blog with malicious script. This is nothing new because we have been watching eBay's team of hacks do this to our sites for over five years now. They typcially try to DDOS or SQL inject our sites because they don't want the truth getting out about the fraud on eBay. Over 90 days of phishing fraud was documented by Doc in 2013 to protect consumers from buying on eBay Motors. If you are unsure of a listing on eBay Motors please download this Vehicle Buying Guide.
There are indeed some very good used car deals on the net. Unfortunately that good deal is usually several states away. If the cars price seems unrealistically low chances are there is a good reason for it. Don't be taken advantage of!
Used Car Buying and Selling Internet Guide – Internet Car Buying Tips Click Here
4 Ways to Spot a Fraudulent Vehicle Listing on eBay Motors
- Newly listed vehicle by a zero feedback seller lists a late model vehicle with a too good to be true price tag.
- Contact information placed in the listing to get you to call and make a wire deposit to hold the vehicle.
- By googling the VIN number to see if it shows up anywhere else online under another seller.
- Using Google Images to see if the images in the listing appear anywhere else online.


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2812 Midwestern Parkway,
Wichita Falls, TX 76308
Phone: (940) 692-1913
Click here for directions
Alexander M. Hanson
September 16, 1944 - July 30, 2020
Alexander M. Hanson, 75, formerly of Wichita Falls, passed away on Wednesday, July 30, 2020, in Big Spring, Texas.The family will receive friends at a visitation from 6:00 until 7:00 p.m. on Tuesday, August 4th at Lunns. Graveside services with military honors will be at 10:00 a.m. on Wednesday, August 5th at Hillcrest Memorial Park in Ardmore, Oklahoma, with Pastor Jim McCool officiating. Arrangements are under the direction of Lunn's Colonial Funeral Home. A son of the late Helen (Morton) and L.W. Hanson, Alexander was born on September 16, 1944, in Cheyenne, Wyoming. He served his country proudly in the United States Air Force. He enjoyed fishing, camping and watching westerns on television.Along with his parents, Alexander was also preceded in death by a granddaughter, Rhonda Irene Hanson; and his favorite dog, Buddy.He is survived by his sons, Gayland Hanson of Newalla, OK; Garland Hanson of Ada, OK; Randall Hanson and wife, Linda of Big Spring, TX; and Glendall Hanson and wife, Jean of Wichita Falls; sister, Marion Sadler of Ardmore, OK; grandchildren, Carolyn Hanson of Paragould, AR; Richard Hanson of Amarillo, TX; David Gray of Mulberry, AR; Randall Wayne Hanson of Lawton, OK; MiKayla Hanson of Big Spring, TX; Jason Hanson of Big Spring, TX; April Smith of Randlett, OK; Christopher Smith of Wichita Falls, TX; Angel Gray of Wichita Falls, TX; Crystal Wolf of Moore, OK; and Christopher Gray of Wichita Falls, TX; and numerous great-grandchildren; and nieces and nephews.For those desiring, memorial contributions may be sent to the Veterans Administration.
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March 21, 2020
Peter Sidwell
We've been back in the test kitchen and we have been getting creative with California Prunes to reduce fat within recipes. We have captured these prune Korean meatballs in our studio this week. It's all about keeping amazing flavours whilst reducing fats in recipes. Le...
March 19, 2020
Experimental baking is key part of our working day. Our development kitchen has been busy working on alternative approaches to baking both in fat and sugar reduction. Both these elements are key to flavour, mouth feel, shelf life and texture. This is quite a challengin...
November 3, 2019
Well I have now launched my latest new business www.jam-eater.com its a platform that allows businesses to subscribe to my food content library. I have been creating lots of new and exciting recipes for both myself and my many clients from all around the world. Click h...
July 4, 2019
Lavender and lemon curd shortbreads
Prep time 20 minutes
Bake time 15 minutes
Makes 8-10 biscuits
Chocolate and orange sables
250g butter (room temperature)
125g icing sugar
1 medium egg yolks
1 tbsp of lemon curd
375g plain flour
1 tbsp vanilla extract
1 tbsp of...
May 23, 2019
I cooked this dish recently during one of my Symphohny Group kitchen demonstrations in Perth Scotland, it when down a storm with lots of requests for the recipe so here it is!
Chimichuri Steak
Serves 4
4 6oz steaks of your choice, I suggest sirloin, hanger st...


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West Shallowford farmstead shown on 19th century maps. Shallowford was mentioned in 1400
Devon County Council, 1838-1848, Tithe Mosaic, approximately 1838-1848 (Cartographic). SDV349431.
Several buildings shown on 19th century Tithe Map on both sides of a track. The Apportionment for 'West Sholleford' lists Field Number 1584 as 'House & Garden'.
Ordnance Survey, 1880-1899, First Edition Ordnance 25 inch map (Cartographic). SDV336179.
'West Shallowford' shown on 19th century map as a group of buildings on either side of a long irregular shaped yard.
Gover, J. E. B. + Mawer, A. + Stenton, F. M., 1932, The Place-Names of Devon: Part Two, 530 (Monograph). SDV337894.
Shallowford was mentioned as 'Sholeford' in 1400.
White, P., 2013, Previously Unsurveyed Dartmoor Historic Farmsteads, West Shallowford (Un-published). SDV352501.
West Shallowford farmstead shown on the Tithe Map.
Ordnance Survey, 2015, MasterMap (Cartographic). SDV357601.
West Shallowford shown on modern mapping.
Search results generated by the HBSMR Gateway from exeGesIS SDM Ltd.


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Pather Panchali not available on your favorite video service? Find something else you'll like!
Impoverished priest Harihar Ray, dreaming of a better life for himself and his family, leaves his rural Bengal village in search of work. Alone, his wife, Sarbojaya, looks after her rebellious daughter, Durga, and her young son, Apu, as well as Harihar's elderly aunt Indir. The children enjoy the small pleasures of their difficult life, while their parents suffer the daily indignities heaped upon them.
Pather Panchali streaming: where to watch online?
Currently you are able to watch "Pather Panchali" streaming on Kanopy, Criterion Channel, Eros Now, HBO Max. It is also possible to buy "Pather Panchali" on Apple iTunes, Vudu, Amazon Video, Google Play Movies, YouTube as download or rent it on Apple iTunes, Vudu, Amazon Video, Google Play Movies, YouTube online.


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Labour's shadow chancellor has refused to rule out her party's support for a "wealth tax" on assets to aid the country's coronavirus recovery.
Anneliese Dodds refused seven times to discuss the detail of a policy that could involve taxes on savings or property ownership in the UK to boost the country's public finances.
Speaking on the BBC's Andrew Marr programme and Sky's Sophy Ridge on Sunday, Ms Dodds said the burden of higher taxation ought to fall on those with the "broadest shoulders".
But pressed on what a Labour wealth tax might involve, she said a debate over the policy would take place only if the UK has a severe Covid-19 recession.
"I think, where there needs to be additional funds going into the system, they should be coming from those with the broadest shoulders, and we have seen an increase in income and wealth inequality over recent years," she said.
"I think there's strong public support for where there needs to be that additional contribution, that coming from those with the broadest shoulders."
Her comments come in advance of an economic statement from Rishi Sunak, the Chancellor, on Wednesday.
The "mini-Budget" will allow the Government to set out policy for a coronavirus recovery.
Asked if she was a socialist, Ms Dodds said she wanted to see a "fairer society".
"We need to change to that," she said.
"Some people would call that socialism; some people would call it fairness.
"What's most important to me is that we actually get that changed that we don't ever enter a crisis again, with so many families in our country struggling."
Ms Dodds has previously said the Government "does need to look at" the idea of a wealth tax, which could see the Treasury raid accounts for taxpayers' savings.
Basic rate taxpayers can currently earn £1,000 of interest on their savings tax-free, and higher-rate taxpayers can earn £500.
Speaking to the BBC's Andrew Marr on Sunday, Ms Dodds said the wealth tax was a "complicated area".
"There's a large number of academics who are looking at this actually, [there are] currently a variety of different proposals," she said.
"And I would really encourage Government to engage with that rather than looking at tax rises, which would affect everyone equally. I don't think that would be fair."
Ms Dodds, who first entered Parliament in the 2017 election, took over the job of Shadow Chancellor from John McDonnell when Sir Keir Starmer entered office as Labour leader.
Labour is widely expected to abandon much of the economic policy developed under Jeremy Corbyn, which called for free broadband, a pensions tax and significant nationalisation of industry.


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Performer: Devolution
Title: Self-Made Monster
Released: 1999
Style: Death Metal, Industrial
Rating: 4.5
Votes: 776
Other formats: XM ASF MP2 MP4 APE MP1 AAC
Band Name Devolution (USA). 1. Self Made Monster. 2. Crack Rocks in the Engine Block. 5. Dance of the Dead. 7. Mistriss of Desire.
Devolution (4) – Self-Made Monster. Label: Wickedisc Records – none. Country: US. Released: 1999. Self-Made Monster.
Self Made Vol. 1 is a compilation album by MMG. The album was released on May 23, 2011, by Maybach Music Group and Warner Bros.
Self Made Monsters Music, Lyrics, Songs, and Videos by Self Made Monsters at ReverbNation. Album Sweet Sixteen Anniversary Show. Self Made Monsters added a new photo to the album Sweet Sixteen Anniversary Show - with Steven Johnson. 14 June 2011 ·. Jeff Clayton announcing the band. Self Made Monsters added 19 new photos to the album Sweet Sixteen Anniversary Show. 13 June 2011 ·. Sweet Sixteen Anniversary Show.
A Self-Made Monster book. By turns violent and funny, A Self-Made Monster is one of the most unusual vampire novels ever published. It strips away the tired cliches and purple prose of the vampire story and replaces them with dead-on characterizations, a galloping plot, and a new conception of the vampire itself.
However, there are also many differences. The most important of these is that the UK Parliament remains sovereign in law and can still legislate, in theory and in fact, for Scotland, Wales and Northern Ireland.
|Self-Made Monster
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Perspective ARTICLE
A New Design for the Fixed Primer Regions in an Oligonucleotide Library for SELEX Aptamer Screening
- Department of Chemistry, Marshall University, Huntington, WV, United States
Single-stranded DNA or RNA oligonucleotides, often called aptamers, can bind to a molecular target with both high affinity and selectivity due to their distinct three-dimensional structures. A technique called systematic evolution of ligands by exponential enrichment (SELEX) is used to screen aptamers from a random DNA or RNA pool, or library. The traditionally-designed oligonucleotides in libraries contain a randomized central region along with a fixed primer region at each end for amplifying target-bound central sequences. The single-stranded forward and reverse primer sequences may interfere with target-binding to the central region, resulting in a partial or complete loss of high-affinity aptamers during the SELEX process. To address this issue, researchers have modified the traditional oligonucleotide libraries and developed new types of oligonucleotide libraries; however, these approaches come with various limitations. The author proposes a new design that uses a conformation-changeable sequence as primers, which may open a new avenue for developing an optimized aptamer sequence with both high affinity for, and selective binding to, a particular target via SELEX.
Aptamers are single-stranded DNA or RNA oligonucleotides that bind to a molecular target with both high affinity and selectivity due to their distinct three-dimensional structures. Target-specific aptamers are mostly screened from a random DNA or RNA pool using a technique called systematic evolution of ligands by exponential enrichment (SELEX), which involves repetitive rounds of (1) partitioning target-bound aptamers from those not bound to the target, and (2) amplifying target-bound aptamers by the polymerase chain reaction (PCR).
Since its emergence in the 1990s, the conventional SELEX technique has undergone substantial modifications and improvements to allow the aptamer screening process to be both simpler and more efficient (Berezovski and Krylov, 2002; Mendonsa and Bowser, 2004; Berezovski et al., 2005). For example, coupling the high resolving power of capillary electrophoresis (CE) to traditional SELEX produced a substantial improvement (Mendonsa and Bowser, 2005; Mosing et al., 2005). The CE-SELEX process occurs in free solution (i.e., no need for a solid support medium), isolates high-affinity aptamers in just two to four rounds, and does not require negative screening. This flexible and high-resolution approach has allowed the successful screening of aptamers capable of targeting both macromolecules such as proteins, and small compounds such as porphyrin (Yang and Bowser, 2013; Dong et al., 2015).
The traditionally-designed DNA or RNA oligonucleotides in libraries contain a randomized central region of 30–60 nucleotides, and a fixed primer region of ~20 nucleotides at each end. The target molecule tightly binds to the central region of some of the library's fragments. However, the single-stranded forward and reverse primer sequences, which are used to amplify target-bound central sequences via PCR, may also interact with/anneal to complementary sequences in the central region, thus interfering with target-binding and resulting in a partial or complete loss of high-affinity aptamers. Furthermore, the primer sequences can participate in non-specific selection by binding to the target themselves, thus leading to false-positive results since the screened aptamers (i.e., the central sequences) do not actually bind to the target molecule (Pan and Clawson, 2009). The fixed primer regions of the oligonucleotides in libraries thus play a central role in limiting the efficiency of SELEX aptamer screening.
To address this issue, researchers have attempted to modify the traditional oligonucleotide libraries or develop new types of oligonucleotide libraries. For example, Haynes' group has modified the standard nucleic acid library by introducing nucleotides that are complementary to the fixed primer regions of the library's fragments, thus eliminating the possibility of the single-stranded primer regions becoming involved in forming any aptamer-like structures (Ouellet et al., 2014). This strategy, however, has a drawback: Since some protein families (e.g., transcription factors) preferentially bind to duplexed nucleic acids, the now double-stranded primer regions in Haynes' design create binding sites for those proteins.
Other strategies include replacing the fixed primer regions with different sequences after each round of screening or after a certain number of rounds (Shtatland et al., 2000), completely removing the fixed regions and then regenerating these regions after each round of screening (Wen and Gray, 2004; Jarosch et al., 2006; Pan et al., 2010; Lai and DeStefano, 2011), or minimizing the length of the fixed primer regions (Pan et al., 2008). These approaches have drawbacks as well, such as the involvement of many steps of cleavage and re-ligation, which is time-consuming and labor-intensive, and often causes a significant loss of the library material; or the apparent decrease of the PCR efficiency due to the reduced length of the forward and reverse primer sequences, which causes the loss of the screened aptamers.
To the author's knowledge, no study has investigated the use of highly structured, self-folded forward and reverse primers to replace the standard single-stranded primers. This may be because the self-folded primer regions are aptamer-like structures themselves, and are more likely to bind to target molecules than single-stranded primer sequences. However, the use of primers that change their conformation under different conditions may actually lead to a higher selection of the target molecule. In this study, the author proposes this new idea for primer design to be used during SELEX aptamer screening.
First, as illustrated in Figure 1, the binding buffer is adjusted to Condition 1, so that the forward and reverse primer regions can self-fold into a highly structured conformation. Previous studies have demonstrated that, unlike in the conventional SELEX method, the target may bind to the randomized central region of the oligonucleotide library and/or to the folded primer regions. This is because there is little or no sequence homology among the aptamers identified using the CE-SELEX approach (Mosing et al., 2005; Yang and Bowser, 2013). Next, the target-bound fraction is separated from the unbound library, which is achieved on a CE system; the bound fraction is then collected into a vial. Since the bound fraction includes unwanted aptamers (i.e., those in which only the primer regions bind to the target), the buffer in the collecting vial is then adjusted to Condition 2, so that the self-folded primer structures unwind and the bound target is dislodged. Aptamers that use their central regions to bind to the target are not affected under Condition 2 if their central motifs do not undergo a conformation change that would release the target. The third step is to re-inject the collected solution onto the CE system, and further separate the target-bound aptamers from the unbound ones under Condition 2. This final screening produces only aptamers that have the target bound to their central regions.
Figure 1. Schematic illustration of the new design for the fixed primer regions in an oligonucleotide library to be used for SELEX aptamer screening.
This new primer design requires the use of conformation-changeable DNA or RNA sequences as primers. A potential choice for such a primer is an intercalated motif (i-motif). The i-motif is a four-stranded quadruplex structure formed by cytosine-rich DNA and RNA sequences. A cytosine nucleobase may protonate at its N3 position under slightly acidic conditions, thus allowing the formation of a C+:C base pair between one protonated and one unprotonated cytosine via three hydrogen bonds. The C+:C base pairs hold two parallel cytosine tracts together, which intercalate in an antiparallel-oriented C+:C duplex to form a four-stranded i-motif (Abou Assi et al., 2018). The stability of an i-motif depends on factors such as the pH and ionic strength of the solution, the DNA or RNA sequence, the length of the cytosine tract, the length and nucleobase composition in the loop regions (i.e., the nucleotides that connect the cytosine tracts in an i-motif), and the temperature. By adjusting the ionic compositions and the ionic strength of both the binding and separation buffers used in the SELEX process, modifying the length of the four cytosine tracts in an i-motif, and altering the length and/or the nucleobase composition in the loop region, an i-motif can stably fold at physiological pH (~7.2) (Fujii and Sugimoto, 2015; Gurung et al., 2015; Reilly et al., 2015; Benabou et al., 2016; Wright et al., 2017). The folded i-motif structure is disrupted and becomes single-stranded when exposed to a higher pH (e.g., ~8.0). The use of aptamers designed to include i-motifs with somewhat different sequences as the forward and reverse primers in an oligonucleotide library allows the differentiation of the 5′ and 3′ ends of the oligonucleotides for PCR purposes. With this design, the target-binding process first occurs at Condition 1 (i.e., pH ~7.2); the self-folded i-motif primers do not interrupt the binding of a target molecule to the central region of the library's fragments. The bound aptamers are then separated from the unbound library fragments by CE and collected into a vial containing a buffer at approximately pH 8.0 (Condition 2). The high pH causes any target molecules that bound to the i-motif primer regions in the previous step to be dislodged due to the pH-mediated conformational change of the i-motif. The collected solution is then re-injected onto the CE system, and the bound aptamers are separated from the unbound ones at Condition 2 (i.e., pH ~8.0). Phosphate buffer is a candidate for the CE running/separation buffer, as it can be adjusted to both pH 7.2 (Condition 1) and 8.0 (Condition 2), without losing essential buffering characteristics; an uncoated fused-silica capillary is sufficient to run the entire experiment under both pH conditions. The aptamers screened using this new approach will have a high affinity to the target molecule at both physiological pH and a higher pH (~8.0).
Studies have demonstrated that C-rich i-motifs and G-rich G-quadruplexes can co-exist if one or both are stably folded (Phan and Mergny, 2002; Kumar et al., 2008; Bucek et al., 2009). In the new design described above, the self-folded i-motif primers that exist under Condition 1 will not interact with any G-rich sequences in the randomized central region since the base-pairing energy for the C+:C pair in an i-motif (169.7 kJ/mol) is much higher than that of canonical Watson-Crick GC pair (96.6 kJ/mol) (Yang and Rodgers, 2014). Under Condition 2, the i-motif primer regions become single-stranded; however, the likelihood that they would interrupt the identification of G-quadruplex aptamers in the central region is low because G-quadruplex motifs can stably fold under higher pH (~8.0). In addition, the target of interest has already bound to the central region of certain library fragments under Condition 1. If, under Condition 2, some of these potential aptamers lose their affinity for the target due to pH-induced conformational changes or interaction with now single-stranded primer sequences, our goal of only screening aptamers with high affinity to the target molecule at both physiological pH and a higher pH (~8.0) is achieved.
In addition to the i-motif that responds to a change in pH, other potential primers include DNA or RNA motifs that change their conformation in response to changes in temperature, or to changes in the composition of the binding buffer, such as a change in the concentration of either Mg2+ or a monovalent ion. The author asserts that this new type of conformation-changeable primer may render more flexibility for developing an optimized aptamer sequence with both a high affinity for and selective binding to a particular target under different conditions, and could thus be the future direction for the construction of libraries developed for SELEX aptamer screening.
Data Availability Statement
All datasets generated for this study are included in the article/supplementary material.
Author Contributions
The author confirms being the sole contributor of this work and has approved it for publication.
This work was supported by the National Science Foundation under Award No. OIA-1458952. Any opinions, findings, and conclusions expressed in this material are those of the author and do not necessarily reflect the views of the National Science Foundation.
Conflict of Interest
The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Abou Assi, H., Garavis, M., Gonzalez, C., and Damha, M. J. (2018). i-Motif DNA: structural features and significance to cell biology. Nucleic Acids Res. 46, 8038–8056. doi: 10.1093/nar/gky735
Benabou, S., Garavis, M., Lyonnais, S., Eritja, R., Gonzalez, C., and Gargallo, R. (2016). Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure. Phys. Chem. Chem. Phys. 18, 7997–8004. doi: 10.1039/c5cp07428b
Berezovski, M., Drabovich, A., Krylova, S. M., Musheev, M., Okhonin, V., Petrov, A., et al. (2005). Nonequilibrium capillary electrophoresis of equilibrium mixtures: a universal tool for development of aptamers. J. Am. Chem. Soc. 127, 3165–3171. doi: 10.1021/ja042394q
Berezovski, M., and Krylov, S. N. (2002). Nonequilibrium capillary electrophoresis of equilibrium mixtures–a single experiment reveals equilibrium and kinetic parameters of protein-DNA interactions. J. Am. Chem. Soc. 124, 13674–13675. doi: 10.1021/ja028212e
Bucek, P., Jaumot, J., Avino, A., Eritja, R., and Gargallo, R. (2009). pH-Modulated Watson-Crick duplex-quadruplex equilibria of guanine-rich and cytosine-rich DNA sequences 140 base pairs upstream of the c-kit transcription initiation site. Chemistry 15, 12663–12671. doi: 10.1002/chem.200901631
Dong, L., Tan, Q., Ye, W., Liu, D., Chen, H., Hu, H., et al. (2015). Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein using CE-SELEX. Sci. Rep. 5:15552. doi: 10.1038/srep15552
Fujii, T., and Sugimoto, N. (2015). Loop nucleotides impact the stability of intrastrand i-motif structures at neutral pH. Phys. Chem. Chem. Phys. 17, 16719–16722. doi: 10.1039/c5cp02794b
Gurung, S. P., Schwarz, C., Hall, J. P., Cardin, C. J., and Brazier, J. A. (2015). The importance of loop length on the stability of i-motif structures. Chem. Commun. 51, 5630–5632. doi: 10.1039/c4cc07279k
Jarosch, F., Buchner, K., and Klussmann, S. (2006). In vitro selection using a dual RNA library that allows primerless selection. Nucleic Acids Res. 34:e86. doi: 10.1093/nar/gkl463
Kumar, N., Sahoo, B., Varun, K. A., Maiti, S., and Maiti, S. (2008). Effect of loop length variation on quadruplex-Watson Crick duplex competition. Nucleic Acids Res, 36, 4433–4442. doi: 10.1093/nar/gkn402
Lai, Y. T., and DeStefano, J. J. (2011). A primer-free method that selects high-affinity single-stranded DNA aptamers using thermostable RNA ligase. Anal Biochem, 414, 246–253. doi: 10.1016/j.ab.2011.03.018
Mendonsa, S. D., and Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Anal. Chem. 76, 5387–5392. doi: 10.1021/ac049857v
Mendonsa, S. D., and Bowser, M. T. (2005). In vitro selection of aptamers with affinity for neuropeptide Y using capillary electrophoresis. J. Am. Chem. Soc. 127, 9382–9383. doi: 10.1021/ja052406n
Mosing, R. K., Mendonsa, S. D., and Bowser, M. T. (2005). Capillary electrophoresis-SELEX selection of aptamers with affinity for HIV-1 reverse transcriptase. Anal. Chem. 77, 6107–6112. doi: 10.1021/ac050836q
Ouellet, E., Lagally, E. T., Cheung, K. C., and Haynes, C. A. (2014). A simple method for eliminating fixed-region interference of aptamer binding during SELEX. Biotechnol. Bioeng. 111, 2265–2279. doi: 10.1002/bit.25294
Pan, W., and Clawson, G. A. (2009). The shorter the better: reducing fixed primer regions of oligonucleotide libraries for aptamer selection. Molecules 14, 1353–1369. doi: 10.3390/molecules14041353
Pan, W., Xin, P., and Clawson, G. A. (2008). Minimal primer and primer-free SELEX protocols for selection of aptamers from random DNA libraries. Biotechniques 44, 351–360. doi: 10.2144/000112689
Pan, W., Xin, P., Patrick, S., Dean, S., Keating, C., and Clawson, G. (2010). Primer-free aptamer selection using a random DNA library. J. Vis. Exp. 41:2039. doi: 10.3791/2039
Phan, A. T., and Mergny, J. L. (2002). Human telomeric DNA: G-quadruplex, i-motif and Watson-Crick double helix. Nucleic Acids Res. 30, 4618–4625. doi: 10.1093/nar/gkf597
Reilly, S. M., Morgan, R. K., Brooks, T. A., and Wadkins, R. M. (2015). Effect of interior loop length on the thermal stability and pKa of i-motif DNA. Biochemistry 54, 1364–1370. doi: 10.1021/bi5014722
Shtatland, T., Gill, S. C., Javornik, B. E., Johansson, H. E., Singer, B. S., Uhlenbeck, O. C., et al. (2000). Interactions of Escherichia coli RNA with bacteriophage MS2 coat protein: genomic SELEX. Nucleic Acids Res. 28:E93. doi: 10.1093/nar/28.21.e93
Wen, J. D., and Gray, D. M. (2004). Selection of genomic sequences that bind tightly to Ff gene 5 protein: primer-free genomic SELEX. Nucleic Acids Res. 32:e182. doi: 10.1093/nar/gnh179
Wright, E. P., Huppert, J. L., and Waller, Z. A. E. (2017). Identification of multiple genomic DNA sequences which form i-motif structures at neutral pH. Nucleic Acids Res. 45, 2951–2959. doi: 10.1093/nar/gkx090
Yang, B., and Rodgers, M. T. (2014). Base-pairing energies of proton-bound heterodimers of cytosine and modified cytosines: implications for the stability of DNA i-motif conformations. J. Am. Chem. Soc. 136, 282–290. doi: 10.1021/ja409515v
Yang, J., and Bowser, M. T. (2013). Capillary electrophoresis-SELEX selection of catalytic DNA aptamers for a small-molecule porphyrin target. Anal. Chem. 85, 1525–1530. doi: 10.1021/ac302721j
Keywords: systematic evolution of ligands by exponential enrichment (SELEX), aptamer screening, oligonucleotide library, fixed primer regions, i-motif
Citation: Wang B (2020) A New Design for the Fixed Primer Regions in an Oligonucleotide Library for SELEX Aptamer Screening. Front. Chem. 8:475. doi: 10.3389/fchem.2020.00475
Received: 10 January 2020; Accepted: 07 May 2020;
Published: 05 June 2020.
Edited by:John D. Wade, University of Melbourne, Australia
Reviewed by:Taiichi Sakamoto, Chiba Institute of Technology, Japan
Yu Xia, Jiangnan University, China
Copyright © 2020 Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Bin Wang, email@example.com


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Welcome to our Classroom Website
My name is Mr. Kohnen This is my nineteenth year in the Anoka-Hennepin school district. I am looking forward to teaching the first 3rd grade class ever at Sunrise Elementary. Thank you for supporting your student's education! I am excited to work with your child and watch their growth. Together we can help your child achieve success!
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Romans 3:27-30
J.B. Phillips New Testament
Romans 3:27-30
J.B. Phillips New Testament
Faith, not pride of achievement
27-28 What happens now to human pride of achievement? There is no more room for it. Why, because failure to keep the Law has killed it? Not at all, but because the whole matter is now on a different plane—believing instead of achieving. We see now that a man is justified before God by the fact of his faith in God's appointed Saviour and not by what he has managed to achieve under the Law.
29-30 And God is God of both Jews and Gentiles, let us be quite clear about that! The same God is ready to justify the circumcised by faith and the uncircumcised by faith also.Read full chapter
J.B. Phillips New Testament (PHILLIPS)
The New Testament in Modern English by J.B Phillips copyright © 1960, 1972 J. B. Phillips. Administered by The Archbishops' Council of the Church of England. Used by Permission.


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Korff Wealth
Complete Rebrand
the brief
We were thrilled when we received the brief to work on the rebrand and name change for the former, Yellow Brick Road Wealth and Management, Northern Rivers, which is now operating under Korff Wealth. The business saw an opportunity to restructure and rebrand during the current COVID-19 crisis. With the current economic climate, Australians need expert financial advice they can trust, more than ever. And so, it was the perfect time to strategically review the business and position for future growth.
our response
It was time for Yellow Brick Road Wealth Management to rebrand and restructure and position themselves in the market as Korff Wealth. With Barefruit's client services and branding expertise, we made this happen with a complete rebrand. The team worked with the client on the strategic direction for the brand's future as well as the visual aspects, including a new brand name, brand image, and brand logo and brand-new website which was designed by our creative lead and put together by our digital team who assisted in organising, uploading and optimising content. As well as completely rebranded both offices Ballina and Lismore.
To inform the public of Korff Wealth's rebrand, Barefruit created an integrated campaign, that combined both online and traditional advertising. We placed online ads in GIF format to capture the attention of people searching for financial advice. This was complimented with a newspaper article and press ads that were scheduled in the local Northern Star and The Ballina Advocate over a period of four weeks as well as other collateral.
the fruits
The result is a slick, and professional rebrand that the client is very happy with. Korff Wealth, with its new name, new logo, brand-new website, new social media pages, and signage for the Ballina and Lismore offices will certainly be attracting old and new customers looking for an expert, objective financial advice across wealth management, insurance and lending.
The newspaper article about the Korff Wealth rebrand was immediately picked up by Newscorp (one of Australia's largest media conglomerates) which ran the article across nine of their publications from Bundaberg to Gladstone; Stanthorpe to the Whitsundays; Byron to Lismore and in between.


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Our Showroom is closed to the public from 6 August COVID-19 UPDATE >
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845 N Las Palmas Avenue
845 N Las Palmas Avenue, Los Angeles, California 90038 United States
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- Beds: 3
- Baths: 4.00
- sqft building: 2652
- sqft lot: 7287
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Belgian Organic Blonde (5,6°)
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Yet another sociological concepts is David Colquhoun's. Colquhoun felt that the important cause of someone's behaviour was his sanity. He also believed the surroundings where he grew up influenced how he participates.
Still another famous sociological theory is John Rawls'. This sociological theory deals with the differences between men and women, as well as between the rich and the poor. It is very much related to the issue of wealth inequality. People who are wealthy generally believe that their wealth should be used to improve the lives of the poor, but others believe that wealth, because of its importance in society, should remain within the hands of the rich.
There are still a number of sociological theories that are being devised. One case is Steven Pinker's. Pinker's idea says that our intelligence is the consequence of many distinct genes, but not just one, so letting us understand precisely the notion of their"nature vs. http://www.bu.edu/info/admissions-overview/graduate-admissions/ nurture" debate.
Many people are still studying the question of the sociological theories. When one begins to study sociology, there are so many questions that one will wonder about. For example, if every interaction between two people is viewed as a competition, then why is there such a lack of competition between people? Sociologists also try to answer questions like, why do we have families, why do we spend so much time together, and why do people get married?
A amazing instance of sociology in nursing is just one of the many very great books on psychology by Suzy Bednarczuk, Ph.D.. She is a professor of nursing in Portland State University. She focuses on the question of if breastfeeding is excellent for child ladies, or if it's awful for infant boys. She discovered, in some instances, breast feeding was beneficial for babies of both sexes.
While they're work, she found that, in most cultures, females are encouraged to stay in house with their kids, and often times, their husbands move out of their home out. The simple fact she identified breastfeeding to become very theraputic to both boys and girls is really a superior illustration of a theory in nursing can be useful.
Since you may observe, the area of sociology has lots of vital places, including criminal justice, cultural diversity, and even gender differences. Sociological theories in nursing will permit you to acquire insight into your field of attention. Think of it for a back up want to bettering yourself.
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Tue 22 May 2007
MassMoca Tarps
Posted by eph under MassMoca, North Adams at 7:28 am
Great NYTimes article on the resolution of a hotly disputed exhibit in the enormous gallery at MassMoca. Even before I reached the last few paragraphs of this article, I was guessing this dispute was fabricated, all part of the experience. Maybe I've just seen too much contemporary art. I wouldn't be shocked if MassMoca was in on it: the tarps practically scream of conceptual art, particularly given the themes of this exhibit. And even if unplanned, the tarps will undoubtedly come to be viewed as part of the art by critics.
Speaking of North Adams, the Mohawk will be opening sooner rather than later: should provide another huge boost for downtown. For a great summary of recent changes to downtown North Adams, including photos, read this recent Berkshire Eagle article.Print • Email
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2 Responses to "MassMoca Tarps"
outed anon says:
North Adams! Ah, the memories. Back in the days of Beacon st and tending bar at Grannies. The Aces, quite a place to hang out!
Great to hear that the old cinema is going to be back online. Hard to believe that the porches on river st is a top notch motel. Those that invested ten years ago are now getting their just rewards. Cudos to the mayor. Always controversial, always enforcing his will, and always looking out for North Adams!
Anonymous says:
Actually this is a very very real dispute. I got to see the exhibit and met with the curator for a class. It's also a damn good exhibit, even if the artist was an egomaniac.
You can follow this conversation by subscribing to the comment feed for this post
If a comment you submitted does not show up, please email us at eph at ephblog dot com. Please note that commenters are required to use a valid email address when submitting comments.


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Concept 058
Share of Wallet
The wallets in this series were developed specifically to illustrate the share-of-wallet concept, but they can be used to tell other stories as well: payment made and received, reduction in disposable income, etc. The fat wallet and skinny wallet can also be used to help tell parts of the concept. Alternatively, the emphasis can be off the cash and on the credit cards. There are two credit cards in each pouch in the wallet that can be formatted to look like whatever credit card, identification card, or membership card you wish.
The wallet and currency are drawn in layers so the wallet can be empty or have the dollar bills appear to be within the wallet. It's all about layering, and that's why only PowerPoint downloads are offered for this series. The download has the back of the wallet layered to the back. The currency is just above the back of the wallet. This means that you can move the stack of currencies into the wallet easily.
This concept works well with animation. Download the example animation and see one possible way of using animation to underscore the message.
The download has both a realistically colored wallet and also a tone-on-tone version. Tone on tone works well if you want to minimize the presence of the wallet on the page or if you want to keep to your template's color scheme. Refer to the tutorial for more formatting tips.
See to012_v02 for a gauge that illustrates a share-of-wallet concept as well.
Key search words: share of wallet, expenses, income, fat wallet, skinny wallet, spending
Tutorial for Interactivity with One Object
Keywords: share of wallet, expenses, income, fat wallet, skinny wallet, spending
Categories: Concepts
Links to instructions for getting the framework into your presentation
- Determine the best file type for your needs
- Download the file
- Import the framework into your presentation
Frameworks available in this series
Click on a name for more information.
Click on a thumbnail to view a larger image


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Author has written 3 stories for War, Nature, and General.
Hello world of Fiction Press!
I'm Andy Devoe, an aspiring writer that loves books, music, and coffee. I am a college student, so I don't have much free time to write, but take every opportunity when I have the chance. I love to read fantasy, horror, and most fiction. Ray Bradbury, Stephen King, and Brandon Sanderson our my favorite authors.
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Thanks for dropping by!


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Close-up of the upper part of the shingled wall. The new piece of decorative wood has also been installed.


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Before you hit submit, check out the Submission Policies page for answers to questions you may have. Continue reading
Tag Archives: applications
RCDeCade: A Case Study to Show NIH Support Trends in an Emerging Scientific Field
A few weeks ago, we touted the value of the NIH's Research, Condition, and Disease Classification (RCDC) system to give us consistent annual reporting on official research budget categories and the ability to see trends in spending over time. RCDC's robust scientific validation process, which allows for such consistency, provides public transparency into over 280 different NIH budget categories.
RCDC categories do not encompass all types of biomedical research. So, how can we get this type of data for other research areas that are not encompassed in RCDC categories, especially those which are newly emerging fields? Are we able to use the same thesaurus-based classification system to explore other research trends?
Continue reading
"Cover Letters and their Appropriate Use" Podcast Now Available
Ever wonder what you should and shouldn't put in a grant application cover letter? Dr. Cathleen Cooper, director of the Division of Receipt and Referral in NIH's Center for Scientific Review, explains just that in the latest addition to our "All About Grants" podcast series – "Cover Letters and Their Appropriate Use" (MP3, Transcript). Continue reading
Do Reviewers Read References? And If So, Does It Impact Their Scores?
In March 2017, we wrote about federal funders' policies on interim research products, including preprints. We encouraged applicants and awardees include citations to preprints in their grant applications and progress reports. Some of your feedback pointed to the potential impact of this new policy on the peer review process. Continue reading
Be Careful to Pick the Right Funding Opportunity Announcement (FOA)
Recent policy changes requiring clinical trial applications to be submitted to FOAs that specifically allow clinical trials, first announced in fall of 2016, impact how all NIH applicants choose a FOA, whether you are submitting a clinical trial or not. Continue reading
Applications, Resubmissions, and the Relative Citation Ratio
Measuring the impact of NIH grants is an important input in our stewardship of research funding. One metric we can use to look at impact, discussed previously on this blog, is the relative citation ratio (or RCR). This measure – which NIH has made freely available through the iCite tool – aims to go further than just raw numbers of published research findings or citations, by quantifying the impact and influence of a research article both within the context of its research field and benchmarked against publications resulting from NIH R01 awards.
In light of our more recent posts on applications and resubmissions, we'd like to go a step further by looking at long-term bibliometric outcomes as a function of submission number. In other words, are there any observable trends in the impact of publications resulting from an NIH grant funded as an A0, versus those funded as an A1 or A2? And does that answer change when we take into account how much funding each grant received? …. Continue reading
Outcomes of Amended ("A1") Applications
In a previous blog, we described the outcomes of grant applications according to the initial peer review score. Some of you have wondered about the peer review scores of amended ("A1") applications. More specifically, some of you have asked about … Continue reading
Resubmissions Revisited: Funded Resubmission Applications and Their Initial Peer Review Scores
"My first submission got an overall impact score of 30. Is that good enough? What's the likelihood I'll eventually get this funded?", or, "My first submission was not even discussed. Now what? Does anyone with an undiscussed grant bother to … Continue reading
R01 and R21 Applications & Awards: Trends and Relationships Across NIH
As described on our grants page, the R21 activity code "is intended to encourage exploratory/developmental research by providing support for the early and conceptual stages of project development." NIH seeks applications for "exploratory, novel studies that break new ground," for "high-risk, high-reward studies," and for projects that are distinct from those that would be funded by the traditional R01. R21 grants are short duration (project period for up to 2 years) and lower in budget than most R01s (combined budget over two years cannot exceed $275,000 in direct costs). NIH institutes and centers (ICs) approach the R21 mechanism in variable ways: 18 ICs accept investigator-initiated R21 applications in response to the parent R21 funding opportunity, while 7 ICs only accept R21 applications in response to specific funding opportunity announcements. As mentioned in a 2015 Rock Talk blog, we at NIH are interested in trends in R01s in comparison to other research project grants, so today I'd like to continue and expand on looking at R01 and R21 trends across NIH's extramural research program. …. Continue reading
Are You On the Fence About Whether to Resubmit?
When applicants receive their summary statement resulting from the review of an application that was assigned a score outside of the ICs funding range, there are important decisions to be made that, ideally, should be based upon evidence. What is the likelihood that an application like this one will be funded? If I resubmit the application, what changes might improve the chances for a successful resubmission?
Recall that in 2014, NIH relaxed its resubmission policy (OD-14-074) to allow applicants to submit a new (A0) application following an unsuccessful resubmission application. Also, we recently posted a piece showing that review outcomes for new applications submitted following an unsuccessful resubmission had about the same funding success as other new applications. But some applicants may wonder, what is the funding success for a resubmission application? …. Continue reading


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This is the 954-796 subcode (NPA-NXX) prefix page. Follow a prefix to find the number you are looking for. We divide our pages up to make them more user friendly to navigate!
The 954-796 subcode mainly covers Coral Springs in Broward county. But other numbers maybe included as well.
We have a database of information derived from the public phone records of Florida state. Many of the numbers in this subcode are Regular Landline registered to At&t Southeast. We hear they employ a large number of people in the Florida office.
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