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Storm Watch: Hurricane Sandy
Mon, Oct 29, 2012 - 01:49pm#21
robie robinson
Status Gold Member (Offline)
Joined: Aug 25 2009
Posts: 1020count placeholder
well i thought i was prepped
raised by my depression era G-parents we've always lived a "prepped" life, however, this am. the windshield wiper blade,drivers side,decompensated on my way into the office. I got soaked putting the passenger side where it would be useful. robie
Sager, hat tip to the Giants. How's this for resiliency: GoooOOO Lions!
Seriously now, I have been glued to this storm, I wish all well, and have mentally gone through all possible scenario's and the preps are done, duplicated and backed up. The greatest comfort I can think of is having installed a tri-fuel carburetor to my whole house generator. Gasoline, propane and natural gas can be used, are stored, and the system is portable.
Good Luck Folks
Mon, Oct 29, 2012 - 03:07pm#23
Adam Taggart
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Joined: May 25 2009
Posts: 5087count placeholder
Red Cross shelters
Google has posted an interactive map with all sorts of data on Hurriance Sandy. The red pins on the map represent Red Cross shelters where you/loved ones can ride out the storm if your current location becomes endangered.
Click on the map to zoom in/pull up information about a location:
The map also has a toggle that shows where the worst of the storm surge is expected to take place. If you live in a coastal area, play it safe.
Don't wait until things look bad to leave. If you wait to do so, beyond your own life, you end up endangering those who may be called in to help you (I am having this issue right now with an elderly family member who is resisting leaving her seaside home).
So far so good…just had 1 quick power outage, power came right back on. Definitely not as bad as Irene last year, so far. I think it has a lot to do with the lack of leaves on the trees – they are all blowing around my backyard.
I think landfall has been moved up to 5PM now. Since the storm has turned towards New Jersey and is probably at it's closest to point to me in West-Central CT, it may not get much worse than this…fingers crossed.
Very responsible Adam, and the best this community can offer. I pray your family member is moved to safety.
I can help (financially), would like to help, and if I can do this directly just let me know. Are there any PP members directly volunteering where I might be able to send a donation?
In the final analysis, the Great Lakes may be affected as badly as anywhere. I mentioned earlier a friend who lives on Lake Ontario. He has talked to a lot of people up there who are lifetime residents. None remember anything like the confluence of a tropical hurricane and a system coming in from the west. I did some searching online and found a study done by the Corps of Engineers around 1990 looking at historical weather events on L. Ontario. The summary mentioned a number of storm types, the directions they came from and the seasons when these types of storms occurred. There was nothing indicating a storm like this had occurred in the historical record. Then I called a friend who works for the Corps and has access to buoy readings. Wave heights on Ontario are now about 5 ft, but forecasts are predicting over 20 ft waves some time tonight. There are supposed to be 25 ft waves at the south end of Lake Michigan (Chicago). The houses along the stretch of shoreline where friend #1 lives are generally no more than 10 ft above the waterline.
The bottom line is that the Great Lakes may be very unprepared for what is currently brewing. Give a thought to friends and relatives who live near the Lakes, particularly the south sides/ends of those lakes.
Mon, Oct 29, 2012 - 07:32pm#27
Chris Martenson
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Joined: Jun 07 2007
Posts: 5220count placeholder
It's starting to blow pretty good here…
We're having our first 30+ mph gusts here in central MA. I've already had a power flicker event so I am not terribly sanguine about our chances of keeping it on over the next 48 hours (!!) while the storm is going to chug around in our region.
Yet another Halloween shaped by mother nature, last year's being affected by the freak snowstorm that took our power for 6 days.
I topped off our gasoline jugs because I do not like how Sandy is pointed right at a significant portion of our east coast refining capacity. All that has to happen to create real shortages around here is for one of those refineries to go off line for a couple of weeks.
Given that they are already going to be (at best) essentially off line for a few days because they went into preventative shut down in anticipation, I think fuel stands a decent chance of going in short supply as a result of this storm.
The early pictures out of NJ and lower NYC already tell a tale of significant flooding and damage and the main portion of the storm has yet to arrive right at high tide. I am certainly glad I am not 100% reliant on the NYC subway system as that might be offline for a while if it gets flooded as now seems likely.
I do tend to take such storms with a grain of salt because of all the hoopla that the media likes to generate, but this one…I don't know…it's that central pressure of only 940 mb … that's really, really low. I know she's not very well formed, which is an oddity given that central pressure, but I think the chance of organizing and strengthening is there given the fuel she's got and the low pressure reading.
At any rate, I will post this while I can using my main systems, but am prepared to have to switch over to my cell phone and laptop backup system. After that I have to resort to my local wifi coffee shop 'system' and if that's not working the library in the next large town system. 🙂
Sitting in my office in southeastern New England watching through the patio doors as the rain and wind pummel the trees below and on the ridge across the valley. Nothing too bad so far, just a little more intense than a normal fall or spirng rain noreaster. Schools are canceled and most everything is shut down. Very few calls or emails all day. Lights have flickered several times but so far so good. We are worried after last year, living through Irene, which didn't come withing 150 miles of here, but left downed trees, damaged builidngs and no electricity for a week.
Left our retreat in the northern New England woods yesterday to return to town home to be with kids, grandkids, elderly parents and to batten down the hatches. Place up there should be out of range. It felt strange looking for flashlights and going to the emptied market last night. In the woods we are all set for these events. Wood stove heats whole house (and can be used to cook) (Three cords piled up behind house, another cord on porch.) Propane cook stove for meals and hot water. Dug well and buckets provide water when drilled well's pump goes down. Battery backup to run computer, small lights,cd and dvd players, or to charge cell phones (has hand crank for use if stored charge runs down.) Enough canned and boxed food to last a week or two without problem. When we were going over our list of "to do" things for final preparations this morning I told my wife that next event we should stay up north where we are good to go when disaster hits.
Mon, Oct 29, 2012 - 07:59pm#29
Wendy S. Delmater
Status Diamond Member (Offline)
Joined: Dec 13 2009
Posts: 1434count placeholder
meanwhile, in NYC
…where I used to work as a safety manager, a tower crane is dangling off of the soon-to-be tallest residential building in Manhattan – 157 West 57th Street. Imagine trying to deal with a partially collapsed crane in this weather.
New York City officials have order evacuation of the upper floors of several nearby buildings. Those things can be lethal. Click on the link for another photo and the full story.
6:30 pm. So far, so good in Philly. Not as much rain (yet) as I expected. Didn't like the way the tree in our sidewalk out front was flapping against the power line that comes into the house, so we did some trimming this morning in the 25 mph gusts. Problem solved. Neighbor's massive maple out back (overhanging our deck and rear of house) doesn't seem to mind the wind at all. Wind is 32 mph steady with gusts above 40 mph. Electrical power hasn't even flickered. City was closed today and will be so again tomorrow.
"May the odds be ever in your favor."


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Zarb-e-Azb: Security forces kill 10 terrorists in Datta Khel, NWA
PESHAWAR (92 News) – In another successful raid on militant hideouts in the ongoing Operation Zarb-e-Azb, the security forces Thursday killed at least ten terrorists in the Datta Khel area of North Waziristan Agency.
According to the Inter Services Public Relations (ISPR), Pakistan Army gunned down as many as ten militants in an operation in Datta Khel area. The forces are continuing search operation in the area.

subset: commoncrawl


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Posttranscriptional regulation of interleukin-10 expression by hsa-miR-106a
Edited by David W. Talmage, University of Colorado Health Sciences Center, Denver, CO, and approved February 11, 2009 (received for review September 3, 2008)
This article has a Correction. Please see:
IL-10 is a key regulator of the immune system that critically determines health and disease. Its expression is finely tuned both at the transcriptional and posttranscriptional levels. Although the importance of posttranscriptional regulation of IL-10 has been previously shown, understanding the underlying mechanisms is still in its infancy. In this study, using a combination of bioinformatics and molecular approaches, we report that microRNA (hsa-miR-106a) regulates IL-10 expression. The hsa-miR-106a binding site in the 3′ UTR of IL10 has been identified by site-directed mutagenesis studies. Also, the involvement of transcription factors, Sp1 and Egr1, in the regulation of hsa-miR-106a expression and concomitant decrease in the IL-10 expression, has also been demonstrated. In summary, our results showed that IL-10 expression may be regulated by miR-106a, which is in turn transcriptionally regulated by Egr1 and Sp1.
IL-10 is a key orchestrator of the immune system that has been shown to be antiinflammatory in many model systems (1). It is secreted by various cell types like different T cell subsets, macrophages, dendritic cells (DC), B-cells, mast cells, and eosinophils. It mediates a plethora of immunoregulatory events such as maturation and activation of macrophages and DC, expression of MHC-II and B-7, activation of T cells, synthesis of cytokines, and antibody production (2, 3). Also, dysregulation of IL-10 leads to various immunological diseases, such as cancer, rheumatoid arthritis, asthma, infectious disorders, etc. (2). Therefore, it is likely that IL-10 expression is tightly regulated.
Regulation of IL-10 expression has been studied at the transcriptional and posttranscriptional levels. Although Sp1 and Sp3 have been found to regulate transcription, it has been shown that IL10 mRNA is constitutively transcribed in many cells; however, the availability of its protein level is significantly determined by posttranscriptional mechanisms (4, 5). AU-rich elements (ARE) in the 3′ UTR of mouse IL10 that lead to the degradation of its mRNA have been shown (6). Half life of IL10 mRNA in normal melanocytes is much shorter than in melanoma cell lines (7). Also, genetic variations in the 3′ UTR of IL10 have been shown to be associated with IL-10 levels that could lead to disease pathogenesis (8, 9). Therefore, a pellucid understanding of the posttranscriptional regulation of IL10 will be of scientific and clinical significance.
A growing class of noncoding RNAs called microRNAs (miRNAs) is involved in posttranscriptional regulation of genes (10). MicroRNAs have been reported to modulate hematopoietic lineage differentiation (11), angiogenesis (12), cell adhesion (13), etc., indicating that they could have important roles in numerous biological processes (14). There is also a growing body of literature supporting the potential role of miRNAs in regulation of inflammatory processes (15). The importance of IL-10 in the orchestration of the immune response, and the strong evidence for its posttranscriptional regulation, makes it an attractive candidate for miRNA mediated regulation of inflammation.
Here, we report the identification of a miRNA, hsa-miR-106a, that regulates IL-10 expression. We also find that Sp1 and Egr1 have an important role in hsa-miR-106a transcription and, thus, indirectly regulate the expression of IL-10 posttranscriptionally.
Prediction of miRNA Regulators of IL-10.
To predict the miRNA that may regulate IL-10, we used a consensus approach by employing 3 different miRNA target prediction software, as described in Materials and Methods. We found that 8 miRNAs have potential binding site in the 3′ UTR of IL10 (Table 1). Also, using miRex, we found that 5 miRNAs (namely hsa-miR-106a, miR-106b, miR-20a, miR-20b, and miR-93) were expressed in cells of lymphoid and myeloid origin, which are known to express IL-10. Because information regarding the expression of the remaining 3 miRNAs was not available, we focused our study on hsa-miR-106a, miR-106b, miR-20a, miR-20b, and miR-93.
Identification of hsa-miR-106a As a Regulator of IL-10 Expression.
To identify the miRNA(s) that may be involved in the regulation of IL-10, initially Raji cell line was chosen, because it constitutively expresses high level of IL-10 and, thus, presumably may have lower level of regulatory miRNA(s). When these cells were transfected with the 5 predicted miRNA oligonucleotides, hsa-miR-106a oligonucleotide significantly down-regulated the expression of IL-10 present in the culture supernatant (Fig. 1A). Interestingly, hsa-miR-106b, which differs from hsa-miR-106a in only 2 nucleotide positions, also affected IL-10 expression, albeit at a lower level. In contrast, transfection with other 3 miRNA or cel-miR-67, a miRNA from Caenorhabditis elegans having no sequence identity with the predicted human miRNAs (used as negative control), had no effect on the IL-10 expression (Fig. 1A). Also, when increasing concentration of hsa-miR-106a was used, IL-10 expression was found to be reduced in a dose-dependent manner (Fig. 1B).
To check whether hsa-miR-106a affects the stability of IL10 mRNA, RT-PCR was performed by using RNA prepared from Raji cells transfected with increasing concentration of hsa-miR-106a oligonucleotide. It was observed that it down-regulated IL10 mRNA in a concentration-dependent manner (Fig. 1C), whereas cel-miR-67 had no effect. Thus, it is likely that hsa-miR-106a decreases IL-10 expression by degrading its mRNA.
To confirm the involvement of miR-106a further, we chose Jurkat cells that produce low basal level of IL-10, and, thus, may produce higher level of hsa-miR-106a. When Jurkat cells were transfected with increasing concentration of anti-hsa-miR-106a oligonucleotide and IL-10 levels were measured in the culture supernatant, a dose-dependent increase in the levels of IL-10 was observed (Fig. 1D). In contrast, a random oligonucleotide had no effect on IL-10 expression. Thus, these experiments demonstrate that hsa-miR-106a regulates IL-10 expression.
Expression of hsa-miR-106a and Its Involvement in IL-10 Regulation in Different Cells.
To specifically detect the expression of hsa-miR-106a, we standardized Northern blotting conditions so that we specifically detect hsa-miR-106a, not hsa-miR-106b (Fig. 2A). Next, when blot was performed using RNA prepared from A549, Jurkat, Raji, HeLa, Hep-G2, and THP-1 cells, hsa-miR-106a was found to be expressed significantly in Jurkat, Raji, and THP-1 cells (Fig. 2B). However, Hep-G2, HeLa and A549 cells expressed lower amounts of hsa-miR-106a. To correlate the expression of hsa-miR-106a with 3′ UTR regulatory activity, Jurkat, Raji, THP-1, and A549 cells were transfected with pMIR-REPORT-IL10 3′ UTR (intact). A substantial fall in luciferase activity was observed in Jurkat, Raji, and THP-1 cells. In comparison, the decrease in A549 cells was not profound. Thus, the down-regulatory effect of IL10 3′ UTR was found to be correlated with the levels of mature hsa-miR-106a present in these cells (Fig. 2C).
Confirmation of Target Site for hsa-miR-106a in IL10 3′ UTR.
Our bioinformatics analysis predicted the binding of hsa-miR-106a to the 3′ UTR of IL10 in a region encompassing +4451 to +4478 bases (Fig. 3A). When luciferase reporter vector containing the intact 3′ UTR was transfected in Jurkat or Raji cells, a significant down-regulation in the luciferase expression in both cells with respect to control was observed (Fig. 3B). This down-regulation was not seen in mutant 3′ UTR construct lacking the predicted 27 base hsa-miR-106a binding site. Also, when A549 cells, which expresses minimal level of hsa-miR-106a, were cotransfected with hsa-miR-106a oligonucleotide and pMIR-REPORT-IL10 3′ UTR (intact), we observed >2-fold reduction in luciferase expression (Fig. 3C). However, cotransfection with cel-miR-67 did not have any appreciable effect on luciferase expression. Most importantly, when hsa-miR-106a was cotransfected along with pMIR-REPORT-IL10 3′ UTR (mutant), there was minimal reduction in the luciferase expression. Also, cotransfection of pMIR-REPORT-IL10 3′ UTR (mutant) with cel-miR-67 had no effect on luciferase expression (Fig. 3C). These experiments indicate that mature hsa-miR-106a regulates IL-10 expression by interacting with its 3′ UTR.
Transcriptional Regulation of hsa-miR-106a.
Because the level of hsa-miR-106a was found to vary in different cell lines (Fig. 2B), and this variation could be due to differential regulation of its expression, we sought to investigate the transcriptional regulation of hsa-miR-106a locus present on the X chromosome. Thus, a region encompassing 1 kilobase upstream of the putative transcription start site (TSS) of hsa-miR-106a was analyzed by using Transfac Alibaba prediction software (16). Two transcription factors, Egr1 and Sp1, were predicted to bind to this region (Fig. 4A). When this 1 kilobase putative promoter region was cloned upstream of the luciferase gene (pGL3 basic, a promoter less vector) and transfected in Jurkat cells, we observed a 4-fold induction in the luciferase expression (Fig. 4B); thus, indicating that the putative promoter sequence possessed regulatory activity.
To establish the binding of putative transcription factors Sp1 and Egr1, we determined the levels of these proteins using Western blotting of nuclear extracts prepared from unstimulated or stimulated Jurkat cells (PHA/PMA combination for 2 h). Although the basal level of Egr1 in Jurkat cells was undetectable, there was a significant increase after stimulation with PHA/PMA combination (panel 1 in Fig. 4C). However, the level of Sp1 was unaffected by stimulation (panel 2). To demonstrate the binding of Sp1 and Egr1, EMSAs were performed by using end-labeled oligonucleotide probe from hsa-miR-106a potential promoter region (−285 to −317 bases) and nuclear extract from unstimulated (for Sp1) Jurkat cells. Out of the shifted bands (Fig. 4D, lane 2), our cold chase experiments with miR-106a promoter oligonucleotide (lane 3), irrelevant oligonucleotides NF-κB (lane 4), and Sp1 oligonucleotide (lane 5) indicated the uppermost band (with an arrow) could be Sp1. The specificity of Sp1 band was further confirmed by antibody experiments, where anti-Sp1 antibody abolished this uppermost band (lane 6), whereas anti-Sp3 (lane 7) or normal goat IgG (lane 8) could not.
For Egr1, when nuclear extract from stimulated Jurkat cells was incubated with labeled hsa-miR-106a promoter oligonucleotide, only 1 shifted band was observed (Fig. 4E, lane 3). However, the band was absent when unstimulated nuclear extract was used (Fig. 4E, lane 2). Specificity of the shifted band was confirmed by cold chase with excess of Egr consensus oligonucleotide (lane 4), which abolished the specific band. However, cold chase with Egr mutant oligonucleotide was not able to chase the specific band (lane 5). This observation was further confirmed by antibody super shift experiments, where antibody against Egr1 was able to shift the band (lane 6), whereas non specific IgG did not (lane 7). These experiments indicate that both Sp1 and Egr1 bind at an overlapping sequence in the promoter region, and could regulate the transcription of hsa-miR-106a.
To determine the effect of induced Egr1 expression on hsa-miR-106a level, Jurkat cells were stimulated with PHA/PMA combination for different time intervals, and Northern blotting was performed. It showed that there was an increase in the levels of hsa-miR-106a up to 8 h (Fig. 4F), and its levels declined gradually with time (16 and 24 h). It was also observed that the levels of pre-hsa-miR-106a increased with time up to 4 h, and then slowly declines. Interestingly, the decline of pre-hsa-miR-106a preceded the decline of mature hsa-miR-106a (compare 4 versus 8 h). These results indicate that induced Egr1 increased the expression of hsa-miR-106a.
Activation of hsa-miR-106a Promoter Regulates IL-10 Expression.
To correlate Egr1 mediated activation of hsa-miR-106a promoter with IL-10 expression, Jurkat cells were stimulated with PMA, PHA, or PMA/PHA combination for 2 h, and expression of Egr1 was detected by Western blotting (Fig. 5A). It was observed that although PMA (50 ng/mL) alone did not affect Egr1 levels, PHA (5 μg/mL) was able to induce it. However, it was the combination of both PMA/PHA that was able to maximally induce Egr1 levels (Fig. 5A). Next, to determine the level of induced hsa-miR-106a, Jurkat cells were treated with PMA, PHA, or PMA/PHA combination for 8 h. Stimulation with PMA/PHA combination but not PMA alone induced hsa-miR-106a expression (Fig. 5B). This upregulation could be inhibited by specifically knocking down Egr1 expression by using Egr1 siRNA before stimulation (Fig. 5 C and D).
To correlate the level of Egr1 induction with the levels of IL-10, Jurkat cells were treated with PHA, PMA, or PHA/PMA together for 24 h, and IL-10 was measured in the culture supernatant by ELISA (Fig. 5E). It was observed that Jurkat cells induced with PMA alone resulted in 3.5-fold increase in IL-10 expression (Fig. 5E, lane 3), whereas PHA alone or PMA/PHA together significantly reduced IL-10 levels (Fig. 5E, lanes 2 and 4). Thus, the level of IL-10 was inversely correlated with the level of Egr1-induced hsa-miR-106a expression.
The posttranscriptional regulation of IL-10 is a subject of paramount clinical interest because of the crucial role of IL-10 as a modulator of inflammation. In this study, we found that hsa-miR-106a has a key role in regulating IL-10 expression. We provide a combination of in silico and in vitro evidence including a series of transfection experiments with various potential miRNAs in support of this conclusion.
Our finding of differential level of hsa-miR-106a (Fig. 2B) in various cells supports miRNA mediated control of IL-10 is operational in cells that actively produce it. Recently, it has been demonstrated that regulatory T (Treg) cells have a miRNA profile distinct from conventional CD4 T cells, and some miRNAs, including hsa-miR-106a, were found to be down-regulated (17). In our study, the identification of hsa-miR-106a that regulates IL-10 expression further supports this observation. It can also be seen that there is a thin line between regulation and dysregulation of IL-10 expression. It is observed that in 46% of human T cell leukemias, pri-miR-106–363 cluster is overexpressed (18), presumably modulating IL-10 expression that could promote clonal expansion and leukemic cell survival. Our current results with the promoter revealed the presence of overlapping binding sites for Sp1 and Egr1 that may have potential regulatory function for hsa-miR-106a, a member of this cluster.
Sp1 is a well characterized ubiquitous transcription factor that regulates a vast array of genes, including IL-10 (4, 5). The early growth response gene product (Egr1) is a zinc finger transcription factor with a characteristic brisk kinetics of induction. It can either positively or negatively regulate gene transcription in response to stimulation, because Egr1 contains both transactivation and repression domains (19). It has been proposed that Egr1 might be up-regulated in response to environmental challenge. Overlapping binding sites for Egr1 and Sp1 have been previously seen in the promoter of several genes. For example, it has been previously demonstrated that under quiescent conditions the promoters of PDGF-A chain (A) and β-(1)-adrenergic receptor are occupied by Sp1, which regulates basal expression of these genes (20, 21). However, on stimulation, levels of Egr1 rise, allowing Egr1 to displace Sp1 from this region; thus, inducing the expression of PDGF-A as a response to the induction (21). Physical and functional cooperation between Sp1 and Egr1 has also been seen at IL-2Rβ promoter in T cells (22). Here, constitutive level of IL-2Rβ is maintained by Sp1, whereas on induction, Egr1 physically interacts with Sp1 to maximize transcription. Similarly, in our study, Egr1 expression in Jurkat cells resulted in the induction of pre-hsa-miR-106a followed by mature hsa-miR-106a. This finding seems to be in consonance with the observation that Sp1 is present in Jurkat cells at basal levels and may be responsible for basal transcription of this miRNA. Perturbation of this equilibrium by Egr1 would then up-regulate hsa-miR-106a; thus, negatively regulating IL-10. This notion was further supported by our observation that Egr1 stimulated hsa-miR-106a expression was negatively correlated with IL-10 levels. However, the mechanism by which Sp1 and Egr1 cooperate for regulation of hsa-miR-106a expression remains to be elucidated.
PHA and PMA have been known to induce vast array of genes by activating p38 (23), p42/44 ERK (24), and JNK MAP kinases (25). These 3 types of MAP kinases can be activated individually or simultaneously; thus, suggesting their independent signaling roles. Although PMA alone is known to induce the activation of Sp1 in T cells (4), both PMA and PHA have been shown to activate Egr1 in CEM cells (T cell line) (26). IL-10 has been previously shown to negatively regulate LPS-stimulated Egr1 expression in mouse macrophages (27). Thus, it would be interesting to study the effect IL-10 on Egr1 induced expression of hsa-miR-106a.
The regulatory loops and feedback mechanism for miRNA mediated posttranscriptional regulation of IL10 may be critical in maintaining immune homeostasis, and its dysregulation may lead to disease. It seems plausible that 3 regulatory mechanisms like transcriptional regulation, ARE mediated 3′ UTR regulation, and hsa-miR-106a mediated 3′ UTR regulation may critically regulate IL-10 expression. In fact, recently, it has been shown that inhibitory function of NKp46+CD49b+CD3− NK cells recruited in the hepatic granulomas in experimental model of visceral leishmaniasis was correlated with high IL-10 production as a result of increased stability of IL10 mRNA (28). It is very likely that hsa-miR-106a mediated posttranscriptional control could potentially be involved in fine tuning the critical level of IL-10 expression in a context-dependent manner, and hence dictates the outcome of immune response to specific external stimuli.
In summary, our results suggest that IL-10 expression is regulated by hsa-miR-106a, which is in turn transcriptionally regulated by Egr1 and Sp1. Further studies examining the relevance of this regulatory mechanism to human disease are warranted. It would also be interesting to investigate the effect of pathological conditions (like rheumatoid arthritis, cancer, and viral infections, where an altered IL-10 expression is an important aspect of disease pathogenesis) on this regulatory pathway involving IL-10, hsa-miR-106a, and Egr1. Also, identifying other targets of hsa-miR-106a and the effect of activation of Egr1 on other members of the miRNA cluster would be of considerable interest.
Materials and Methods
In Silico Identification of miRNA Binding Sites.
Based on experimental observations of miRNA binding to the 3′ UTR, several target prediction software have been developed. Although each of them has been used extensively for target site detection, they are associated with many false positive results due to lack of specificity of prediction. To avoid the overprediction, we used a consensus approach employing 3 widely-used software to perform the target prediction. Only those miRNA target pairs were identified that were detected by all 3 software to bind to the same location in the target 3′ UTR sequence (29). All known 470 human miRNAs (miRBase v9) were queried against human IL10 3′ UTR using miRanda (30), RNAhybrid (31), and TargetScan (32).
Analysis of miRNA Expression Profiles.
To check which among the 8 miRNAs, predicted to target IL10, were expressed in cells of lymphoid and myeloid origin, we collected data from the miRNA expression resource (miRex; http://miracle.igib.res.in/mirex). This database allows cross-comparison of miRNA expression profile data across various platforms collected from public repositories like Gene Expression Omnibus (National Center for Biotechnology Information-GEO) and ArrayExpress. The prenormalized data retrieved from these repositories are internally normalized to a standard score (z-score) by using an in-house developed platform (miRex; http://miracle.igib.res.in/mirex). Expression of each miRNA in a particular tissue is represented as a fold change. The database currently contains a total set of 1,507 experiments spanning 18 datasets.
Plasmids pMIR-REPORT-IL10 3′ UTR (intact), pMIR-REPORT-IL10 3′ UTR (mutant) and hsa-miR-106a promoter in pGL3 (basic) constructs were prepared as described in detail in SI Materials and Methods.
Cell Culture and Transfection.
A549, Raji, Jurkat, and THP-1 cells were grown and transfected as described in SI Materials and Methods.
Because IL-10 is a secretory cytokine, its levels were detected from culture supernatant of transfected cells by performing ELISA (R&D Systems) following the manufacturer's manual.
RNA Extraction and RT-PCR.
RNA was prepared from cells treated with or without PHA (5 μg/mL), PMA (50 ng/mL), or combination of PHA/PMA (5 μg/mL and 50 ng/mL) for different time intervals by using TRIzol reagent (Invitrogen) following the product manual. RNA was stored at −70 °C until further use. RT-PCR was performed by using specific primers for IL10 and GAPDH following the standard protocol.
The hsa-miR-106a Northern Blotting.
RNA isolated from A549, Jurkat, Raji, THP-1, HeLa, and HepG2 cells were resolved on acrylamide gel, transferred on nylon membrane (Gene screen plus; PerkinElmer), and hybridized with Gamma 32P-labeled hsa-miR-106a oligonucleotide probe, as described in SI Materials and Methods.
Nuclear extracts were prepared from Jurkat cells as previously described (33). These extracts were incubated with radio-labeled double stranded oligonucleotide probes for hsa-miR-106a potential promoter region. Protein-DNA complexes were separated on acrylamide gels. Super shift experiments were performed as described in SI Materials and Methods.
Western Blotting.
Nuclear protein was prepared as described previously (33), and Western blotting was performed for Egr1, Sp1, and Lamin, as described in SI Materials and Methods.
Statistical Analysis.
Results are given as mean of 3 independent experiments ± SEM. An independent 2-tailed Student's t test was performed. Differences were considered statistically significant for P ≤ 0.05. For normalization of transfection efficiency, cells were cotransfected with vector pMIR-REPORT beta-Gal as indicated.
We thank Mr. Nomesh Goplani for technical help. A.S., M.K., and M.H. are supported by fellowships from the Council of Scientific and Industrial Research (CSIR). This work is supported by a grant from CSIR (Task Force Project Grant NWP0033) India.
- 1To whom correspondence should be addressed. E-mail: bghosh{at}igib.res.in
Author contributions: A.S., S.K.B., and B.G. designed research; A.S., M.K., J.A., and M.H. performed research; A.S., M.K., A.A., and B.G. analyzed data; and A.S., A.A., and B.G. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/cgi/content/full/0808743106/DCSupplemental.
Freely available online through the PNAS open access option.
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- Asadullah K,
- Sterry W,
- Volk HD
- ↵
- ↵
- ↵
- Tone M,
- Powell MJ,
- Tone Y,
- Thompson SA,
- Waldmann H
- ↵
- Ma W,
- et al.
- ↵
- Powell MJ,
- Thompson SA,
- Tone Y,
- Waldmann H,
- Tone M
- ↵
- ↵
- ↵
- Kurreeman FA,
- Schonkeren JJ,
- Heijmans BT,
- Toes RE,
- Huizinga TW
- ↵
- ↵
- Chen CZ,
- Li L,
- Lodish HF,
- Bartel DP
- ↵
- Chen Y,
- Gorski DH
- ↵
- Harris TA,
- Yamakuchi M,
- Ferlito M,
- Mendell JT,
- Lowenstein CJ
- ↵
- varez-Garcia I,
- Miska EA
- ↵
- ↵
- ↵
- Cobb BS,
- et al.
- ↵
- Landais S,
- Landry S,
- Legault P,
- Rassart E
- ↵
- ↵
- Bahouth SW,
- Beauchamp MJ,
- Vu KN
- ↵
- Khachigian LM,
- Williams AJ,
- Collins T
- ↵
- Lin JX,
- Leonard WJ
- ↵
- Loza MJ,
- Foster S,
- Peters SP,
- Penn RB
- ↵
- ↵
- ↵
- Cogswell PC,
- Mayo MW,
- Baldwin AS, Jr
- ↵
- Kamimura M,
- et al.
- ↵
- ↵
- ↵
- ↵
- Rehmsmeier M,
- Steffen P,
- Hochsmann M,
- Giegerich R
- ↵
- ↵
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New Zealand is preparing to welcome one of the hottest artists of 2019 for what is sure to be an outrageous live show.
Scottish singer-songwriter Tom Walker is preparing to land in New Zealand for the very first time in August when he will be performing at Auckland's PowerStation for a host of lucky fans.
Walker has had an incredible year so far, performing a sell-out show tour in the UK, winning a BRIT Award for Best Breakthrough Act and reaching platinum status for two of his hit singles. The performer's Kiwi fans will be over the moon to be catching a glimpse of this legendary musician at the top of his game.
Additionally, Walker will be joined by a local artist, Dunedin's Abby Wolfe. The self-taught singer-songwriter has been turning heads around the country with hits such as Win You Over and Change My Mind.
Walker will be performing in Auckland on Tuesday 20th of August and tickets are on sale now, so make sure you don't miss out!


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Audio-Produktion Jobs
Audio production generally denotes all the stages of production right from the actual recording of the audio in the studio to the perfection of the master recording. Audio production involves audio mixing, sound editing, sound design, and the addition of effects. It normally involves several processes, which include: ADR (Automated Dialogue Replacement or Looping), Foley Mixing and Editing, Mixing (also called Re-Recording), Music Composition and Editing, Production Dialogue Editing, Sound Effects Design and Editing, etc. An audio production may require all or some of all these processes in order for it to be complete.
Audio production is used by music producers, video producers, video game producer etc.Audio Producers anheuern
Hello there, I'm working on a project that provides clients with a broad library of simple songs and background soundtracks and I am in need of artists. This is an excellent opportunity for beginning musicians who want to explore their craft, learn their software and experiment with ideas while making a few bucks. -All genres of music welcome: as long as it sounds pleasing to the ear. -Liv...
I am looking for a voiceover artist who has an American or British accent who can do 3 - 5 minute voiceovers. The script will be sent to you.
Animation Sound Design. We need a "natural" sounding music track, on brand for a South African Bank. FX need to be mild and natural sounding to accentuate the animation.
Looking for a male Russia voice talent to record a 30 second commercial. Script has some Russia words so the talent must have a reasonable Russia pronounciation skills. Recordings must be in a very high quality.
We need an American accent voiceover for a small video of 15 seconds, providing us with a voice sample would be helpful thank you
Do you have music that's figuratively collecting dust on your hard drive? Why not sell them to us, and turn them into some $$$ in your pocket? If you want to retain the rights to your tracks, we can discuss a revenue split - the only way for us to get paid, is for YOU to get paid. Let me know if any of this sounds interesting to you. Would be great to hear samples of what you have availa...
We are looking for a voice-over pro to record the English voice-over version for short videos, including the Video Introduction at our [Zur Anzeige der URL Anmelden] web page. We will then adjust the video to match the pace of the voice-over.
edit any interruptions , spaces in between convo, any glitches , any sounds concerns, enhance any issues, delete spaces and empty gaps, correct any echo or sound concern, crop any beg space or ending space .. etc . Clarify the audio to the best possible way .
I am looking for a talent with proven track record to compose a Corporate Music with Vocal Singing. It is for a Thai Food concept in Singapore. Objective of music is to emphasis 1) Authentic. 2) Embody 100 years of history story. 3) Restaurant - Experience. 4) Home Self-Cook Sauce Available that is - Simple, Affordable and Fast.
We have a sad hindi song. which is needed to be composed. Will send you the vocal sample of singer there are 90% chances that you already know him. Approached the label as well.(will send you the proof also.) Here is the requirement 1-: Must be Indian 2- must know hindi well 3-:must mention his/her age and city 4-: must mention his/charge 5-:music should have atleast one live instrument(we ca...
We are a residential redevelopment firm. We buy distressed properties, rehab and sell them. Looking a serious but 'nice guy' delivery. Friendly guy next door ... best I can describe is maybe a sound like the Home Depot voice. Will provide a link to the sample video and copy, once the voice talent has been selected.
need a songwriter to write a song for our company
Looking for a male Russia voice talent to record a 30 second commercial. Script has some Russia words so the talent must have a reasonable Russia pronounciation skills. Recordings must be in a very high quality.
We have 3 x 45 min audio recordings that need to be smoothed, edited to remove gaps and re-statement corrections, add intro/outro music jingle and be production ready for release as a podcast.
Create a lyrics and a melody for an instrumental
artist in search of a musical producer for the creation and edition of instrumental songs Trap/Rap
I am looking for someone to make any future vocal recordings to sound like a perfect finished project. I am a novice to how recording, I have everything to record my song on GarageBand but do not know how all the controls and effects work to make it the perfect finish
[Zur Anzeige der URL Anmelden] need that instrumental (with that loop) to be sounds more like that [Zur Anzeige der URL Anmelden] send me demo version of the song
I have a set of audio files (20 files) which are in .wav format. The recording quality is not that great to use it in a movie. Those audio files are dialogues. I would like someone who has the right skill to clean and improve the audio quality. I am attaching a sample file to look at. Please use that file and improve the quality. If theoutput looks good then I'll award the project.
Looking for a male Russia voice talent to record a 30 second commercial. Script has some Russia words so the talent must have a reasonable Russia pronounciation skills. Recordings must be in a very high quality.
We are looking for a voice actress for a narrative short-film voice-over (290 words script). We are looking for a voice which resembles an age range of between 30 to 40 years old. From an educated background and calm. (character profile) Please send me a link to your portfolio or audio samples through a chat message
Need an experienced audio technician to clean multiple audio files join these files to make one audio file for PODCAST. It might require skills for audio file production. Add royalty free audio to the PODCAST to make it more professional. Remove any background noise etc. If you have produced audio files for PODCAST will be very helpful. I prefer if anyone based in Australia. This will be ongoing w...
i need lyrics writer and i need beautiful songh hindi lyrics writer
You will need to do voice over for a video, we would be taking in samples from you for a video, If it matches with the tone of our fundraiser video, we will be paying you 10$ and will be using your voice for this video which is 3:35 minutes long. But before you bid, you need to write "I accept to provide a free sample voice over: in the bid before you are sleected for the bid conversation. S...
I'm looking to complete Audio Production work. I anticipate the project will last 1 to 3 months. The producer will receive two 3-minute files and will need to mix the files according to a script that will be provided. Sound designer must be able to complete the mixing within two hours from receiving the files.
I need a native English speaker with a native American or British accent (not asian, latino accent) who can work as a voice actor for a Boxing Video Game. A total of 2000 words document will be provided.
We are producing a marketing video and we need a native English speaker with a clear and neutral accent
Solo personas que puedan hablar en español nada de intermediarios
A new song of my mine just released and I need it to be promoted and more listeners audience to the sound track. An expert should send offer of possible solution.
need a female vocals/singing for few projects
Hello, I have recorded my song, I have 3 different audio tracks, one for the vocals, one for the beat and one for the sounds. I need Someone expert with audio songs mixing Wich can help me to have a better result by using autotune and all vocal effects that make the song sound good. I don't have specific questions and guidelines, I trust on the experience of You! thank you
looking for a voiceover talent for youtube videos
Hi There, Im in a project of music production, i would like someone who can produce music tracks to produce some music track for myself, i have the vocals ,must be familiar with up to date styles of music ie arabic style music , production price includes mastering and mixing. Must be a fast and professional worker and be in frequent communication during the production.
I am shifting home and need by Bose Audio to be wall mounted
Create / copy music files. I have 2 music files i like to have created without song. MIDI or MP3 so i can adjust tempo, put up volume on tracks. The sound have to be authentic sound. Please note my budget! Please DONT write if you cant match the budget.
I need a cool track in the style of(Future house , Bass house Midtempo hexagon records style) (Don diablo label) Examples/References [Zur Anzeige der URL Anmelden]
Those interested in the project can send sound samples to me. I'll send the text privately to get the fee.
UK Ascent only. Please dont forget to share your portfolio
I have a few pages of voice over script. I need a confident, good pronunciation and nice voice over recording in a good mic setting. I will send a script and i need in mp3 or other audio formats
I need someone to make unedited song covers sound studio professional and make the voice sound more appealing. Please provide examples of work to get the job
I need someone to take song files and make them sound studio professional. Please provide examples to get the job.
I need audio and podcast expert who can help with the editing and mixing the audio and intro for my upcoming podcast. I would like a concept and music that is both upbeat (preferably electronic) but more so than the specific style I'm more interested in how this is mixed as an intro piece. I've included a few examples of podcast intros that I like as a reference: Podcast ref 1: [Zur ...
I am a 3d animator, working on a 20 seconds clip of dinosaur fighting. I need sound design done for that. It should include a background music + sound effects of dinosaur footsteps, falling on ground, growling, roaring etc.
I'm a piano player, composer and an arranger. Been playing and mastering piano for almost 14 years and I would like to take orders for any of your preferred songs to transcript them into a piano notation, Compose and arrange piano melodies for your favourite songs
I would like someone to do voice overs for a series of videos. This will be an ongoing series of many small videos that require a Greek male or female voice over artist. Samples required
I am looking for an expert voice editor who can fix approximately 30 very short voice clips (most are only a few words long). Currently there is a lot of noise, delays, and other issues.
Im looking for a voice over artist for a crowd funding video
I have an employee who is retiring. One of our traditions for staff retirements before COVID was to sing a farewell song to them at a luncheon. Now with COVID we want to create an online version. I'm thinking we can each film ourselves lip syncing the song (Eddie Money's "2 tickets to paradise") and then have a video editor splice together with music.
I want someone who can get isolated midi files for primary instruments in a song
I would like to find a song writer. I will write words mu self, just need the beat. Beat should be 128 RMP and dance music. The artis who will sing that song is a woman and full of energy. Song should be suitable for live stageses, club and pub.


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RainMeter is the best dps I've even seen.
But it cannot be used in Chine.
Can Chinese Client be support?
Sorry for my pool English.
Chinese support
Post Reply
8 posts • Page 1 of 1
Re: Chinese support
Hi, China or Taiwan? Taiwan client is already working.
Re: Chinese support
It's a private server? I'm planning to add a feature soon so players can manually add any private server to ARM. What's the folder inside L10N?
Re: Chinese support
yes,it's a private server in russia.
L10N have four folders:
but,the name of the skill is in Chinese,
I can supply all the documents you want.
Re: Chinese support
Hmm then they mixed the English data.paks with Chinese, it's a mess to support a modified client like that.
Re: Chinese support
I could work if they use NA English data.pak in L10N/ENG folder and select EuroAion in serverlist
Post Reply
8 posts • Page 1 of 1


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City of Cornwall, Ontario, endorses basic income
By Roderick Benns
Publisher of Leaders and Legacies, a social purpose news site
Often called the poorest city in Ontario, the City of Cornwall in eastern Ontario has passed a motion endorsing basic income guarantee policy.
Less than a month ago, the City of Kingston became the first municipality in Canada to call for the development of a basic income guarantee for all Canadians. Its council unanimously passed a motion calling for a national discussion on the issue, hoping this will lead the provinces and federal government to work together to "consider, investigate, and develop a Basic Income Guarantee for all Canadians."Read more


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Noosa Longboards | Fins | GD6 6" Single Fin
Surf. Aloha. Rock 'n' Roll.
Notify me when this product is available:
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Small centre fin for 2 + 1 set up with large sides, allows you to throw your longboard around and go verticle, also ideal for mini mals with centre box setup. To thrusterize your mal or mini mal. Available in one or two tab base.
(Recommended Side Fins: GPL- 41/2")


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There is a gentleman that works with me at my part-time job in Mortgage that is that rare type of person who can uplift you even on your worst day. He is genuine to the core. It doesn't matter your age, position, background, color etc, he treats everyone with the highest level of respect. He greets passerbys with a courteous "Hello" and sends them off with a "Good day to you". He engages people in conversation, taking a whole hearted interest in their stories. He inspires without the expectation of reciprocation. What an incredible human being.
I can honestly say that I have no complaints about my life lately. I feel incredible. Everything seems to be moving in the right direction for me and my only wish now is that everyone could be just as inspired as I am.
I tip my hat to my coworker and can only hope to embody some of the qualities that he possesses. (Of course I had to pull him aside today and share my opinion with him. I don't know if anyone ever tells him how he makes people feel everyday, but someone has to!)
I know this was incredibly random but I'm feeling inspired and I'm hoping you will to. If there is anyone that touches your life in a positive way, no matter who they are, make sure you tell them!


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IRCU Organizes National Thanksgiving Prayer at State House Entebbeint
On 13th December 2019, the Inter-Religious Council of Uganda (IRCU) organised National Thanksgiving Prayers that were hosted at State House Entebbe under the theme ""Where the other Nine" from Luke 17:17 and Quran 14:7, Q 27:38-40.
The Prayers aimed at thanking God and offer prayers for continued peace in the country and for completing the year 2019.
The event was largely graced by over six hundred guests among which were, the IRCU council of presidents, religious men and women, youth and religious leaders, members of government leadership and members of the diplomatic corp. The Chairperson IRCU council of presidents was represented by the Deputy Mufti of Uganda.
The First Lady and Minister of Education and Sports, Janet Museveni, who led the congregation in a family prayer, thanked God for the nation and asked the Almighty to create a new awareness of His presence in our lives. She also prayed for people to see the light of Christ in this Christmas season.
In his remarks, as the chief host President Museveni noted that it was important to have the custodians of religion to organize the thanks giving service unlike in the past where it had been organized by the "resistance Council" inside state house. He added that Ugandans are better off intoxicated in religion than alcohol.
He highly commended the Inter-Religious Council of Uganda (IRCU) for fostering oneness among the different faiths in the country.
In his sermon, the outgoing Church of Uganda Archbishop, the Most Rev. Stanley Ntagali who was the main celebrant, prayed to God to cleanse Uganda from corruption. He appealed to the media to be agents of development and seek to change people's lives. He further thanked the organizers who thought and gave him a chance to speak to the nation as his tenure of service comes to end.


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Farmers' Market, Wed., July 30th
This week the Dickinson College Farm stand will be overflowing with fresh, certified organic produce. Eggplant, beets, garlic scape vinaigrette dressings, slicing tomatoes, carrots, cabbage, chard, kale, squash, onions, cucumbers, bell pepper, salad mix, herbs, cherry tomatoes… and that's just the start of the list. See you there!
3-7pm at 2 N. Hanover St., Carlisle, PA Check out the Farmers on the Square Facebook page for a weekly list of vendors and market goodies!


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UPDATE: Thursday is the final day to vote for Shackles in the "America's Favorite Crossing Guard" contest. You can vote here.
AUSTIN (KXAN) — Lonnell Shackles sets up shop on a crosswalk near Harris Elementary School in northeast Austin. He's been helping children, parents and passersby safely cross the street for 7 years. Shackles knows the familiar faces by name.
I want to be counted as a positive peg along the road of their life.Shackles
A road he once walked.
"Looking back at my own life when I was growing up… I've always had someone that had a positive effect on my life as I was coming along as a little kid," Shackles said. "I want to be a good representative to all that I meet in the morning and the afternoon."
A representative that could become "America's Favorite Crossing Guard."
He was nominated "for always going the extra mile" to keep people safe, according to a release from the City of Austin.
Shackles said of the nomination:
"Just to have the opportunity to be involved with something like that, I never thought of it. Never suspected it. Didn't even cross my mind," Shackles said. "But for the staff to think enough of me to nominate me to be involved in something like that… (pauses)."
Shackles lost his words but then remembered a story from his past.
I was always the bench person. I didn't mind sitting on the bench, cause I knew at some point in time… I was never on the first team cause I knew some point in time somebody was going to come limping out of the game. The coach was going to look down the bench and say, 'Shackles get in there!' Then I was gonna have my chance, and that's what they gave me. So when they hired me… I'm gonna make the best of it.Shackles
His peers and supervisors agree.
"Lonnell is always keeping an eye out for problems both big and small. Neighbors and families often see him clearing broken glass and debris out of the street — and thanks to his suggestions, students walking to Harris Elementary in the early morning hours received backpack lights to help them get to school more safely," the city wrote in a release.
Shackles is among the 230 crossing guards under Austin Public Works' Safe Routes to School Program, which provides and trains crossing guards who post up around 97 elementary schools in Austin ISD and six nearby school districts.
Safe Kids Worldwide sponsors the "America's Favorite Crossing Guard" contest. The winner will receive $500 worth of new crossing guard gear and accessories and will be recognized with a pep rally at their school.
Last year, two local crossing guards competed in the contest: Thelma James, who monitored an intersection near Andrews Elementary; and Max Poss, who worked an intersection near three Pflugerville ISD schools.


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Are you the kind of person who could go through your closet and find piles and piles of clothes just to complain that you have nothing to wear? We've all been there. What a funny predicament to be in, especially when you still have clothes in your closet that you've never worn. Luckily for you, this is a common issue that has a simple solution — the capsule wardrobe. Creating a capsule wardrobe can seem intimidating, but it's a lot easier than you think! Try these tips from CoverGirl Shapewear to create the most flexible wardrobe to fit all of your fashion needs.
First things first, what is a capsule wardrobe? Well. in short, a capsule wardrobe is a small collection of outfits that mix and match while giving you the most versatile selection of outfits for any daily outing. Keep in mind that this doesn't include your pajamas or workout clothes, but rather the outfits that you can wear to school, work, or really any time that you go out!
Having a capsule wardrobe has a few benefits that make the time and effort spent planning it all worth the work! First, since capsule wardrobes are made with mixing and matching in mind, a few items of clothing can go further in completing an outfit. Because of this, you're sure to spend a lot less money on impulse buys that don't serve at least a few roles within the wardrobe.
Just follow these easy steps and you'll be well on your way to creating a capsule wardrobe.
The first step in creating a wardrobe that is designed to match relies on picking a base color. This base color will tie all of the pieces of clothing together, so choose a common color that you love to wear. Black, Navy, and Beige are all perfect colors to base a wardrobe on. If you're just starting out, you will want to stick to one base color just so you can get the hang of things.
Once you have a base color to go off of, you should focus on getting the essentials down. For the sake of this article, "the essentials" will refer to any article of clothing that is timeless or never goes out of fashion. White button-up shirts, grey t-shirts, jeans, your favorite shapewear, and so on. These are the stables of your wardrobe and they should make up the core of your outfits.
To make things easier, set a number for each kind of item that will go into your wardrobe. Try to keep something like 10 tops, 5 bottoms (pants, skirts, etc.), and 4 or 5 pairs of shoes. This will make your life easier when it comes to shopping and you know just what to purchase.
A basic capsule wardrobe could look like this:
White button up
Blue button
Grey t-shirt
Black t-shirt
White t-shirt
Grey long sleeve t-shirt
Black long sleeve t-shirt
White long sleeve t-shirt
White blouse
Black blouse
Blue Denim Jeans
Black Denim Jeans
Black or Blue Denim Skirt
Black Dress Pants
Finally, update your wardrobe seasonally. Keep the essentials as the core of your wardrobe but update the layers as the seasons change. You can add sweaters, cardigans, and jackets as the weather gets cool.


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November 20, 2019
Medical Devices: a complete support to be in compliance with regulations
Answering the need of Manufacturers and Dealers of medical devices and support them in the…Read More
Thoughts, cool links, inspiration, news and more relating to JSB Solutions activity or pharmaceutical, biotechnology and industrial world. Here you find some relevant updates from our experts and a selection of stuff that professionals would want to know about. If you appreciate what you read, please feel free to share it with your contacts and colleagues via LinkedIn (and make sure to include us via @jsbsolutions).


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Working closely with the buying, packaging and CSR teams, you will have the exciting opportunity to build and shape a strong packaging strategy to deliver best value packaging solutions and plastic commitments. You will be engaging with almost every area of the business, as well as building powerful relationships externally.
The Role
Your Profile
What you can expect
At Wallace Myers International Recruitment Agency we specialise in sourcing professionals for key industries and services. See our website for more details.
Privacy Policy: By applying for this role you are agreeing that your details will be stored in our database and may be considered for similar future positions. For more info see wallacemyers.ie/privacy-policy


text: Research Resources Project Lifecycle PreAward Toolkit Limited Submission Limited SubmissionWhat is limited submission?When a grant sponsor only allows a limited number of applications to be submitted from our institution.What do I do if I want to apply for a grant that has limited submission?Notify your PreAward analyst as soon as possible. If more than one person wants to apply for the same grant, your PreAward analyst will ask you to submit an one-page abstract and a biosketch to Ericia Huff. The Grant Research and Oversight Group will select an abstract and ask that PI to submit an application. Right-Side Nav UTMB Research Experts Network COVID-19 Research at UTMB Grants Management Resources Educational Resources & Presentations PreAward Toolkit PostAward Toolkit Working with the IRB Toolkit Forms Research Blog UTMB Research ListServ Right-Side Nav ECRT eSirius3G Login Funding Institutional infoEd REDCap PeopleSoft Velos TriNetX


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All you need is love and a dog. – Unknown green landscape background,wallpaper water nature
Dogs are better than human beings because they know but do not tell. high resolution nature background, 2560×1440 nature wallpaper
Even the tiniest Poodle or Chihuahua is still a wolf at heart. – Dorothy Hinshaw black wallpaper nature, nature laptop backgrounds
Love is a four-legged word. – Unknown portrait and landscape wallpaper, spring flowers wallpaper hd
I think dogs are the most amazing creatures. The give unconditional love. For me they are the role model for being alive. – Gilda Radner cool nature wallpapers hd, iphone xr hd wallpaper nature
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Yep, Kingdom Hearts has hit me HARD. I guess the hype has been penting up for 12 years and now… here we are.
Because I didn't like the pace, symbolism and what happened at the end of Kingdom Hearts 3, I decided I should go ahead and get rid of this obsession by making a fix-it comic that will also evolve into a SoRiku story with Sora as the King of Kingdom Hearts (can that happen?), Kairi being his High Magician/Priestess and Counselor, and Riku his Knight in shining armor (or maybe in a dark one). Here are the first 8 pages (I hope I can keep on drawing these, because TIME)
A comic in which Sora actually grieves and accepts his sadness as part of himself.
Also, the Destiny Trio will be travelling together and spend some quality time. Also, Kairi will stop being a plot device/damsel in distress and will be a badass. FINALLY.
Another 3.5?
via Tumblr https://ift.tt/2U5osVE


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The Benedictine Rule
Ever since the New Testament epistles were written, Christians have received advice on how to live the Christian life. How much should we pray? What progress can we expect to make in achieving biblical holiness in this life? Is perfection an attainable goal? Is Christianity best lived out in normal circumstances of family, marriage, and vocation, or in hermit-like isolation from others or in communities specially formed for the purpose of cultivating prayer, worship, and work? As persecution of the early church died out and Christians gained freedom of worship in the Roman Empire, the monastic life originated as a … View Resource
Bernard of Clairvaux and Mysticism
One has to appreciate a medieval figure whom Martin Luther and John Calvin looked on with favor and, to a certain degree, approval. The figure in question is Bernard of Clairvaux, a Cistercian monk, abbot, mild mystic, and formidable theologian. It's an understatement to call him an abbot. His monastery eventually founded a daughter institution, then another, then another. By the time of his death, seventy monasteries had been directly planted or started by him, with those institutions responsible for establishing hundreds more. So revered was Bernard that Dante left his faithful Beatrice behind as his guide and had Bernard … View Resource
Boethius: The Philosopher Theologian
One of the least known but most significant Christian thinkers of antiquity was a sixth-century layman called Anicius Manlius Torquatus Severinus Boethius, or simply Boethius for short. The son of an old senatorial family, he lived between 480 and 524, being consul (a largely ceremonial political position) in 510, and then Master of the Offices at the Ostrogothic court in Ravenna in 522. It was while serving in this latter capacity that Boethius was accused of treason, imprisoned, tried, and executed. It remains unclear to this day whether he was actually guilty of treason or, as seems more likely, was … View Resource
Church History in Christ
By the end of the eighteenth century, the church of Geneva had become a mere shadow of its former glory. The pulpit of John Calvin no longer thundered with the bold truths of the Protestant Reformation. It no longer broadcast the Good News of the Scriptures. Instead, it whimpered with the uncertain themes of the Enlightenment. Somehow, it had been mesmerized by the tired and monotonous tones of unitarianism and liberalism. Even the famed Genevan Academy, founded by Theodore Beza, had abandoned the teaching of the Scriptures opting instead for the more fashionable philosophies of Plato, Aristotle, and Seneca. As … View Resource
The Definition of Orthodoxy
The Arian controversy in the fourth century was arguably the greatest theological controversy in the history of the church. As Protestants, we might think that the Reformation controversies of the sixteenth century were the most momentous. Without wishing to minimize their importance, however, the Arian controversy was greater, because it went deeper. The Reformers were arguing about how we receive the benefits of Christ; the men of the fourth century were arguing about something even more basic — who Christ is. Unless a right foundation is laid in the person of the Redeemer, little is gained in disputing about His … View Resource
Faithful Vigilance
Paul warned the elders of the church in Ephesus about the critical need for them to be vigilant: "Pay careful attention to yourselves and to all the flock, in which the Holy Spirit has made you overseers, to care for the church of God, which he obtained with his own blood. I know that after my departure fierce wolves will come in among you, not sparing the flock; and from among your own selves will arise men speaking twisted things, to draw away the disciples after them. Therefore be alert…" (Acts 20:28–31). This apostolic warning was not just for the … View Resource
Fallacious History
One of the most pressing but invisible threats to Christian thinking at the present time is that of fallacious history. Like carbon monoxide, it can kill; you just do not notice it is happening until it is too late. Fallacious history comes in numerous forms. The most obvious and influential are those pushed by popular culture. Movies are the primary culprits here. So powerful are the aesthetics of modern cinema that the stories the movies tell can be compelling for no other reason than that they seem so real. Thus, if there is a movie in which Americans crack the … View Resource
Gospel Footprints
One of the cultural plagues of the twenty-first century is our historical illiteracy. The comedian Jay Leno capitalizes on this when he asks random questions to people. Leno's "Jaywalking" skits demonstrate that regular Americans are not up to speed with the basics of U.S. and world history. In one memorable scene, Leno asked someone to name one of the Ten Commandments. The reply: "Freedom of speech." Enough said. I wonder how well church members would do if someone asked them questions about church history. Would they know the key players, dates, and issues? Does it even matter? Church history does … View Resource
High Crimes and Misdemeanors
Some years ago I caused no little consternation when I was invited to speak at a church on the nature of ministry and started my lecture by declaring that it really did not matter if the pastor was an adulterer or not. As you may imagine, this was not something the congregation had heard before, and my guess is that more than a handful of those present probably thought the speaker had either gone mad or was simply ignorant of the most basic aspects of biblical teaching on the nature of church leadership. View Resource
Historical and Theological Foundations
According to some estimates, there are more than one hundred thousand distinct parachurch organizations in the United States alone. Parachurch organizations are typically defined as Christian organizations that exist alongside (para) the local church without being under the oversight of any specific local church. They are often focused on one particular mission or purpose. There are parachurch organizations devoted to missions (Africa Inland Mission, U.S. Center for World Mission), to Bible distribution (Gideons, Bibles for the World), to discipleship and evangelism (Navigators, Young Life), to publishing (Crossway, Banner of Truth), to various social services (crisis pregnancy centers, homeless … View Resource
Holding the Line
American Protestantism split in two during the 1920s and has not been the same since. In denominational controversies, especially among Presbyterians and Baptists, and in courtroom debates over teaching evolution in public schools, the once unified front of mainline Protestantism, a constituency that included Congregationalists, Presbyterians, Episcopalians, Baptists, Methodists, Disciples, with some Lutherans on the fringes, divided into evangelical and liberal halves. Not until the 1940s, with the formation of the National Association of Evangelicals, would the more conservative side achieve the institutional coherence that characterized the mainline through the Federal Council of Churches (which in 1951 became the National … View Resource
How the Scots Changed the World
The sixteenth-century Scottish divines (pastors and theologians) who labored to build a national church characterized by sound doctrine and biblical worship never realized how far their influence would reach. They aimed, after all, to reform the Kirk, not to change the world. Ultimately, they did both. Their efforts bore fruit not only in a redefined church for the Scots, but in theological commitments, liturgical patterns, social customs, and political persuasions for people around the globe. The extensive impact that the Scottish Reformers had was not due to any real novelty in their beliefs. The men who engineered the reformation of … View Resource
The Lone Monk
The seventh century is something of a forgotten epoch for most Protestants. But it is well worth knowing. The creative heart of its theology lay in the East — the Byzantine Empire, centered on Constantinople. Here the Christological controversies of the fifth century were still bubbling away. As a result of the councils of Chalcedon (451) and of Second Constantinople (553), the Eastern church and empire were bitterly divided between two great parties. These were the Chalcedonians, loyal to the orthodox creed of Chalcedon, that Christ is one person in two natures; and the Monophysites, numerous in Egypt and Syria, … View Resource
Machen's God-Centered Vision
J. Gresham Machen wielded his powers against modernism as an historian and as a student of the New Testament. He argued on historical grounds that from the beginning the church was a witnessing church (Acts 1:8) and a church devoted to the apostles' teaching. In other words, her life was built on events without which there would be no Christianity. These events demanded faithful witnesses who tell the objective truth about the events, since they are essential. Moreover, the life of the church was built on the apostles' teaching (Acts 2:42), the authoritative interpretation of the events. Thus Machen's response … View Resource
The New Mendicant Orders
From the earliest days of the medieval period, bishops were expected to preach regularly as they visited congregations throughout their dioceses, and in their absence, there was broad support for ordained presbyters (elders) to fill the vacancy. As in our own day, however, there was unevenness in the quality and commitment to preaching such that the success of training pastors and keeping them accountable to a high standard had mixed results. What was needed in Europe by the thirteenth century was a fresh infusion of vigor to the preaching of the Word both inside and outside of the walls of … View Resource


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Business interruption coverage varies from policy to policy, but some of the expenses or losses not generally covered by such insurance may include:
Exclusions will be largely dependent on your specific policy and the business interruption-specific language within.
Business owners who have seen their revenues dry up amidst the COVID-19 (coronavirus) economic shutdown may be more interested in what their business interruption insurance may cover more so than what it may not cover.
According to the Insurance Information Institute, it is possible that your business interruption insurance will cover:
Business interruption insurance may be included as part of your property and casualty insurance, which may cover any damage that caused your business to be temporarily shut down, but business interruption insurance could be included in a business owner's policy (BOP).
If your business has been impacted by the mandates stemming from COVID-19, you may have considered what insurance coverage could qualify you for financial assistance. If you have not already, you should:
It may not be immediately clear what your business interruption insurance does or does not cover, even after speaking with a lawyer. There is also a chance that any claim you have filed or will file could be denied. Do not worry, as the laws and insurance precedents surrounding coronavirus and its impact on businesses is far from final.
Business owners have no shortage of headaches and responsibilities during this confusing, uncertain time. As somebody responsible for a business, you may be:
Each of these responsibilities have likely added stress to your plate and consumed an abnormal amount of your time. Calling a lawyer may allow you to:
A lawyer can help you wade through the difficulty of this period.
Most lawyers are familiar with long, carefully-worded documents. This may make them a helpful resource when it comes to interpreting your insurance policies. You may expect a lawyer to:
Many business owners are facing a critical juncture in the future of their company, and you may be in the same boat. A lawyer can help you.
The law surrounding COVID-19-related business losses is far from settled, and precedent for business interruption insurance and the novel coronavirus also remains unclear. It may be in your interest to file a business interruption insurance claim and (perhaps) to take legal action in pursuit of coverage, if such a step is necessary.
Call our team at The Landau Law Group today at (866) 703-4878 to discuss how we may be able to help you fight for your business.


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Boots Shot Glasses - Set of 6
Price is exclusive of GST.
1) Pay with PayPal and get 50% cashback voucher up to Rs. 200 on your eligible purchases
2) Offer is valid only for first time users' first transaction on PayPal and can be availed once per PayPal user
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4) Offer period till 31st of January 2020.
5) Cashback voucher will be credited in user's PayPal account, within 5 days after successful transaction with PayPal.
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Boots take you to good places!
Category: Creative gifts
Sub-Category: Home and Lifestyle
Add style to your boring parties with the Boots Shot Glasses in the same manner as the boots do to your attire. Flaunt these glasses in your circle and become the talk of the town. The distinct design of the glasses will make them the HERO of your parties. With a capacity of 30 ml each, the shot glasses come in a set of 6 pieces styled as boots, a perfect gift for the host of a celebration or a "groom-to-be" or a girl who loves her boots.
- Design: Boot Shaped Glass
- Material: Glass
- Set of 6 glasses
- Capacity: 30 ml each
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1 x Boots Shot Glasses (Set of 6)
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Boots Shot Glasses - Set of 6


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Has a Death Occurred? We Are Available 24/7 (517) 481-3792
Obituary of Gerald F. "Jerry" Boyson
Gerald F. "Jerry" Boyson
Age 88, our loving husband, father, grandfather, and great-grandfather was at home surrounded by family when he was called to his Lord on January 25, 2020. Born January 29, 1931 in Free Soil, MI. Jerry was a meat department manager at A & P Super Markets for 38 years. He was a veteran of the U.S. Marines during the Korean War. He was a member of St. Gerard Church and was a life member of the Knights of Columbus Richard Council #788 where he achieved the 4th degree. He enjoyed gardening, golfing, bowling and watching sports and news on the TV and loved the company of his dogs.
Surviving are his wife of 68 years, Audrey M. Boyson; 5 children Sheryl Hillyer, Roberta (Robert) Fredricks, Gerald "Tom" (Holly) Boyson, Pamela Kintner (Jeff Marketich), and Lynne (Richard) Tosi; 16 grandchildren and 26 great-grandchildren; and many other family members and friends. Jerry was preceded in death by his 2 great-granddaughters; parents, Marie & Fordyce Boyson; 2 sisters, June (Harold) Jacobson, and Mary Jane (Donald) Konicki.
Visitation is from 5:00 – 8:00 P.M. Tuesday with a 7:00 P.M. Rosary and K of C 4th degree Chalice Service in the Tiffany Funeral Home, 3232 W. Saginaw, Lansing. Funeral Liturgy is 10:30 A.M. Wednesday, January 29, 2020 at St. Gerard Catholic Church, 4437 W. Willow, Lansing with Fr. John P. Klein presiding. Viewing will be 9:30 – 10:30 A.M. Wednesday at church. Rite of Comital St. Joseph Cemetery. Military Honors will be provided by Morley S. Oates V.F.W. Post #701. Contributions may be made to St. Jude's Children's Hospital or St. Gerard Church in memory of Jerry. Friends may visit the guest book at www.tiffanyfuneralhome.comTo send flowers to the family or plant a tree in memory of Gerald Boyson, please visit Tribute Store
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The mission of the Sundance Family Foundation is to accelerate youth development and strengthen family stability.
Sundance has had a strong interest in Youth Social Entrepreneurship (YSE) programs—defined as youth-centric, youth-led nonprofit enterprises that include community engagement, social-emotional learning (SEL), and workforce readiness. About 50+ YSE programs currently operate in the greater Twin Cities metro area. Youth are learning social and emotional skills, and community engagement along with 21st Century workforce skills that will lead to their prosperity and independence.
Yet, youth living in low income areas, and youth in communities of color are facing 16-23% unemployment while the average unemployment in Minnesota is 3.2%.
It is now time to focus on creating Earn & Learn pathways. We must no help create a youth focused workforce development strategy that brings all of our youth into exciting careers and livable wages that can both support their families and help them thrive.
Sundance celebrates the 85 Honorees who are currently earning and learning with 65 Community Champions: employers who are hiring new employees at livable wages and paying for their training as they acquire Nationally recognized certificates and degrees. This effort takes a village, one that is being created by many visionary partners.
Sundance is instrumental in:
1. Building Data Capacity: A Completed a 36-month project with Wilder Research support a YSE model to increase personal agency, build social capital and the workforce readiness skills needed for 21st Century InDemand careers. Nonprofit cohort members developed logic models, evaluation plans, installed or refined databases, and collect verifiable evidence-based data. As a result of this work, cohort members could gain access to state and federal dollars that they were not able to access.
2. Bridges and Pipelines: Sundance is helping YSE organizations, the Minneapolis Foundation and GreaterMSP track YSE alumni to ensure that these students find meaningful career paths.
3. Innovative Supports: Sundance is working with scores of public and private sector employers who are helping youth gain and retain employment or early entrepreneurial ventures. Supports include specialized training programs like the one at Hennepin Tech and St. Paul Police Department, Internships and Apprenticeships by Genesys Works and Upturnships, and community engagement activities by organizations like Elpis Enterprise, Urban Roots and Dream of Wild Health.
4. Communicating Bold Innovations: Starting in 2016 Sundance began convening teams of manufacturers, trades, journey, and union representatives with community and academic leaders to build new networks. In 2019 Sundance began creating a documentary on the Earn & Learn efforts of dozens of organizations in the Twin Cities. This film is scheduled to be released in the fall of 2020. Keep an eye on this and social media channels.
5. Wunderkammers: Community Tech and Training Exploratoriums, have been providing unique hands-on career exploration for youth. Wunderkammers were produced in Minneapolis, St. Paul, Vadnais Heights, and Maplewood. Youth engaged with technician in the private and governmental sectors which a focus on jobs available in governments and cities.
Sundance is changing its grantmaking process and dates:
Click HERE for Further Details on Our Application Page
Grants requests are not accepted for International Projects.
Contact Information
Phone: (612) 822-8580
Sundance Family Foundation
944 Grand Ave
St. Paul, MN 55105
Click HERE to download the White Paper outlining the Youth Social Entrepreneurship (YSE) model: combining workforce readiness with social/emotional and community engagement that helps youth and young adults also develop personal agency and social capital.
Sundance Supports an evidence-based approach to sustainable investing. Read the white paper here!
Youth Social Entrepreneurship: Advancing the Field