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Two years after an attempt to overhaul Nevada's public records law died in the Democratic-controlled Legislature, a similar attempt faces uncertain prospects in the final week of the session.
SB287, an omnibus public records overhaul sponsored by Democratic state Sen. David Parks, remains in limbo in the Senate Finance Committee with no hearing scheduled. Meanwhile, two bills that would increase confidentiality in public records — for public retirees and for certain metadata collected by cities — are moving forward.
"It's really disappointing that it's so difficult, especially when we have such a robust preamble to our public records law," ACLU lobbyist Holly Welborn said about efforts to increase transparency, pointing to a public records law that says it aims to "foster democratic principles" and "must be construed liberally to carry out this important purpose."
Parks' bill proposes making a number of changes to the state's public records law including requiring an agency to tell a requester the earliest date and time after which it believes the records will be available, to make a reasonable effort to assist the requester in focusing a request so records can be produced in as timely a fashion as possible, and to provide the name of the person who made the decision about what action will be taken with regard to a records request.
It also would specify that the cost of producing records should only include the cost of ink toner, paper, media and postage and not any labor costs and that the records should be produced electronically unless otherwise requested.
The initial version of the SB287, which carried fines of $1,000 to $250,000 per offense, attracted strong pushback from government agencies. That included an opposition letter that was 12 pages long, plus exhibits, from the Las Vegas Metropolitan Police Department.
"It does not increase transparency. Instead, it burdens the taxpayer by inflicting penalties and immunizing the media from ever having to consider the costs of producing voluminous records," the department wrote. "The bill burdens citizens who have privacy interests in the records we possess. This includes innocent victims of crime, who did not choose to be part of the criminal justice system."
Welborn said that a compromise amendment to the legislation will require that fines paid as a result of noncompliance go to the State Library and Archives and would impose liabilities on someone who's determined to be a nuisance requestor. It also drastically reduces the fine for non-compliance to $500 for a first offense.
"We thought we got the bill to a point where it should be palatable for the rest of the governmental entities," Welborn said. "We're just hoping to get it past that Finance hurdle. We have a lot of coalition members coming together to figure out where that's going and to see if we can get it to the other side as soon as possible."
Parks isn't sure what's going to happen with his bill, which was heard in the Senate Government Affairs Committee but then re-referred to Finance last month without recommendation.
"I'm hopeful that we'll have a hearing on it and move it out so we can get something passed this session," Parks said. "Unfortunately, I'm not, this session, in part of the core inner circle, so I'm just waiting."
Meanwhile, a bill that would close off more data to the public, SB388, got a hearing in the Senate Finance Committee on Monday. The bill proposes exempting certain personally identifiable information collected by government agencies, such as metadata, from the state's public records law.
Proponents of the legislation argue that citizens who are incidentally caught up in data-gathering efforts by municipalities, for instance, shouldn't have their private lives exposed for public consumption. Lobbyist Kelly Crompton testified that the city of Las Vegas has a Smart Cities initiative through which cars with special sensors — such as Audis — transmit data to city infrastructure that helps identify issues such as potholes in the road. The system identifies particular cars, and the city wants to keep those cars' movements private.
But critics take issue with the bill's language, including a portion that allows governments to declare information confidential if its release could cause embarrassment.
"The idea that government gets to use a balancing test to decide whether someone is going to be comfortable or uncomfortable or embarrassed or uneasy or whatever other ridiculous words are in there flies directly in the face of what public records are about," said Republican Sen. Ben Kieckhefer.
Democratic Sen. Mo Denis, the bill sponsor, said he's working to narrow the language.
"I've got an amendment coming. It's going to make the statement that it only applies to Smart City data," he said. "Really all we're talking about is the metadata."
Welborn said that the amendment alleviates some of the concerns the ACLU previously had about the legislation.
"We think consumers should have more privacy rights in that regard," Welborn said. "But it was very scary the way that it came out and was introduced."
Another bill that has made it through the legislative process is SB224, a measure sponsored by Democratic Sen. Julia Ratti that adds more confidentiality to records about the Public Employees Retirement System (PERS). The bill makes information about PERS participants generally confidential, but allows some exceptions.
Initially, the exceptions included an employee's ID number, the years of service and their last employer — notably leaving the person's name out. That drew outcry from groups including the Nevada Press Association.
"The names of those who receive government pensions are vital for detecting abusive practices like double dipping and fraudulent disability claims," wrote press association head Richard Karpel. "And at a fundamental level the taxpayers who serve as the ultimate backstop to the system deserve to know whose retirement they are funding."
After some amendments, the bill now specifies that PERS members' names and pension payouts are public, but information about their last employer and years of service are confidential.
The bill passed both houses on close votes and is now headed to Gov. Steve Sisolak's desk.
Proponents are trying to apply pressure to get SB287 moving, issuing a press release on Monday calling for Senate Majority Leader Nicole Cannizzaro to ask for a vote. The measure hasn't cleared its first committee vote and would still have to make it through the Assembly to pass.
"It does kind of seem like this could be a really great opportunity for leadership to show they are in favor of a transparent government, to strike that balance with some of the other things that are happening so we hope that that's what they decide to do," Welborn said.


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and Manuals We know it's difficult to keep track of your guides, so download any of your Avital® Owner's Guides here. (French), 10/21/, download. Avital Pdf User Manuals. View online or download Avital Owner's Manual. View and Download Avital Model owner's manual online. Avital Owner's Guide Model Model Car Alarm pdf manual download. Also for:
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If warranty service is necessary you must have a clear copy of your sales receipt containing all of the information shown on the following page.
After the first calendar year, from the date of purchase of this auto-security device, Directed Electronics, Avitap. This warranty contains the entire agreement relating to warranty and supersedes all previous and contemporaneous representations or understandings, whether written or oral.
This warranty is void if the product has been damaged by accident, unreasonable use, neglect, avitao service or other causes not arising out of defects in materials or construction. This warranty is nontransferable and does not apply to any unit that has been modified or used in a manner contrary to its intended purpose and does not cover batteries.
The unit 3400 question must be returned to the manufacturer, postage prepaid.
This warranty does not cover labor costs for the removal, diagnosis, troubleshooting or reinstallation of the unit. For service on an out-of-warranty product a flat rate fee by model is charged. Contact your authorized dealer to obtain the service charge for your unit. These systems are a deterrent against possible theft. Directed Electronics does not authorize any person to create for it any other obligation or liability in connection with this security system.
Some states do not allow limitations on how long an implied warranty will last or the exclusion or limitation of incidental or consequential damages. This warranty gives you specific legal rights, and you may also have other rights which vary from state to state. Make sure you have all of the following information from your avjtal A clear copy of the sales receipt, showing the following: Due to the complexity of this system, it must be installed by an authorized dealer only. Installation of this product by anyone other than an authorized dealer voids the warranty.
All dealers are provided with a preprinted dealer certificate to verify authorization. By carefully reading this Owner's Guide prior to using your system, you will maximize the use of this system and its features. Your remote is powered by a small, lightweight 3-volt lithium battery that will last approximately one year under normal use. When the battery begins to avifal, operating range will be reduced and the LED on the remote will dim.
Your product warranty will not be validated if your warranty registration is not returned. Make sure you receive the warranty registration from your dealer. It is also necessary to keep your proof of purchase, which reflects that the product was installed by an authorized dealer. Operation is subject to the following two conditions: Changes or modifications not expressly approved by the party responsible for compliance could void the aivtal authority to operate this device.
Use of this product in a standard transmission vehicle is dangerous and contrary the product's intended use.
This makes it possible to assign any remote transmitter button to any receiver function. The transmitter initially comes programmed with Standard Configuration, but may also be customized by an authorized dealer. The buttons in all of the instructions in this manual correspond to a Standard Configuration transmitter.
Button The disarming function is controlled by pressing this button for one second. An optional auxiliary function, such as trunk release, can be controlled by pressing this button for 1. Button The remote start function of your system is controlled by pressing this button twice within 3 seconds.
Therefore, never operate the system in an enclosed area or partially enclosed area without ventilation such as a garage.
When parking in an enclosed or partially enclosed area or when having the vehicle avita, the remote start system must be disabled using the installed toggle switch.
These precautions are the sole responsibility of the user. Zvital user must also have the neutral safety feature of the vehicle periodically checked, wherein the vehicle must not remotely start while the car is in gear. This testing should be performed by an authorized Directed dealer in accordance with the Safety Check outlined in the product installation guide. If the vehicle starts in gear, cease remote start operation immediately and consult with the authorized Directed dealer to fix the problem.
The remote start module must be removed or the installer must properly reinstall the remote start system so that the vehicle does not start in gear. All installations must be performed by an authorized Directed dealer. When the system arms, you will hear a short siren sound, or chirp, and see the parking lights flash once.
If the power avita locks are controlled by the system, the doors will also lock. While the system is armed, the status LED will flash approximately once per second, indicating that the system is actively protecting your vehicle. 4030
AVITAL MODEL OWNER'S MANUAL Pdf Download.
If you hear a second chirp after arming and note that the status LED is flashing in groups, see the Diagnostics Section of this guide. This extra chirp is called Bypass Notification. If the system is programmed for passive arming, it will automatically arm 30 seconds after the ignition is turned off and the system detects that you have left the vehicle by opening and closing a door.
Whenever the system is in its second passive arming countdown, the status LED will flash twice as fast as it does when the system is armed. At the second point of the countdown, the siren will chirp to indicate that the system is about to arm. At the second point, the parking lights will flash to indicate that the system is armed. If any protected entry point such as a door or a switch-protected trunk or hood is open, the system will not passively arm unless forced passive arming is programmed on.
See Programming Options section. When armed your vehicle is protected as follows: When triggered, the siren chirps and the parking lights flash for a few seconds. Heavy impacts trip a Triggered Sequence. The sequence consists of the siren sounding continuously and the parking lights flashing for a pre-programmed period, which can range in duration from 1 to seconds. If a door is opened, the system will immediately start chirping the siren and flashing the parking lights.
Three seconds later, the siren output changes to a continuous blast. Turning on the ignition key will trip the same progressive response as opening a door. See Table of Zones section of this guide. Pressing only in Standard Configuration again within five seconds of arming the system will activate the Multi-Level Security feature. Each time is pressed again, a different security level is selected. The different security levels are selected as follows: The siren chirps once.
The system is armed. The siren chirps twice followed by a long chirp.
Zone 2 is now bypassed. The siren chirps three times followed by a long chirp. Zone 4 is svital bypassed. The siren chirps four times followed by a long chirp.
Guides and Manuals
Zones 2 and 4 are now bypassed. The siren chirps five times followed by a long chirp. All input zones, except the ignition, are now bypassed. Multi-Level Security Arming only applies to a single arming cycle. Once the system avitla disarmed and then re-armed, all the zones will be active again. Simply press on the transmitter for two seconds while the vehicle is running. The siren will chirp once to indicate that the security system is armed, and then once more to indicate that the ignition is on.
The system will not respond to any input except the door triggers, and the starter kill relay if installed will not be activated. Once you have arrived at your destination, the system will disarm when the ignition is turned off.
The siren will chirp twice and the LED will then stop flashing. The system can also be disarmed at any time by pressing. You will hear two chirps, and the parking lights will flash twice. If the power locks are controlled by the system, xvital doors will also unlock.
The siren chirping either four or five times when disarming indicates Tamper Alert, which is described in the Diagnostics Section of this guide. If the system is triggered and the siren has been sounding for longer than six seconds, pressing on the avitao transmitter will stop the avitl and return the unit to the armed state. The system will not disarm, but rather reset. This prevents you from disabling the system should you wish to disarm it without avktal checking the vehicle.
Pressing after resetting the system will disarm the system; pressing this button during the first six seconds of the triggered sequence will disarm the security system immediately.
The six second timer is provided for your convenience, in case the system is accidentally triggered. Press the Valet button the preset number of times one to DRW five times within 15 seconds.
After five seconds, the system will disarm. If the system does not disarm, you may have waited too aavital turn the ignition off and on and try avitao. The Valet button can be programmed to respond to presses for the disarm function. You must check with the installer to verify the programming for your individual unit. Simply press for less than one second before arming or disarming, and the confirmation chirp s will be eliminated for that one operation only.
This ensures that no chirps will be emitted by the siren in an area you want chirp-free. The system is still fully capable of triggering. Just press for two seconds, and you will enter Panic Mode. The siren will sound and the parking lights will flash for the programmed siren duration.

subset: commoncrawl


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Starlight Suiten Hotel Renngasse
- Free WiFi
- Collect stamps
Very roomy although a bit dated. Good location walkable distance to shopping centres.
Nice hotel with spacious rooms a few blocks away from the main tourist attractions. Not…
Starlight Suiten Hotel Renngassefrom A$178
- Family Suite
- Duplex, 2 Bedrooms
- Classic Suite
- Prestige Suite
- Deluxe Suite, 1 Queen Bed
- Superior Suite
More great choices
What's around Starlight Suiten Hotel Renngasse
- In Innere Stadt
- St. Stephen's Cathedral - 10 min walk
- Spanish Riding School - 10 min walk
- Stephansplatz - 11 min walk
- Hofburg Imperial Palace - 12 min walk
- Albertina - 16 min walk
- Vienna State Opera - 17 min walk
- Naschmarkt - 24 min walk
Getting around
- Vienna Intl. Airport (VIE) - 26 min drive
- Wien Mitte Station - 19 min walk
- Vienna Franz-Josefs-Station - 21 min walk
- Vienna Praterstern Station - 27 min walk
- Schottentor U-Bahn - 5 min walk
- Herrengasse U-Bahn - 7 min walk
- Schottenring U-Bahn - 7 min walk
- Airport shuttle (surcharge)
- Area shuttle (surcharge)
At a glance
Key facts
Hotel size
- This hotel has 52 rooms
- This hotel is arranged over 6 floors
- Check-in time 3:00 PM-midnight
- Check-out time is noon
24-hour airport shuttle service is available on request. Fees may apply. Contact the property in advance to make arrangements.
Required at check-in
- Credit card, debit card, or cash deposit required for incidental charges
- Government-issued photo ID may be required
- Minimum check-in age is 18
Travelling with others
- Up to 2 children (5 years old and younger) stay free when occupying the parent or guardian's room, using existing bedding
- Pets allowed *
- 2 per room
- Free WiFi in public areas
- Free WiFi in rooms
- Airport shuttle (available 24 hours) *
- Self parking *
- Area shuttle *
Payment types at the property
- Cash
Other information
- Smoke-free property
In the hotel
- Buffet breakfast daily (surcharge)
- Bar/lounge
- Coffee shop/café
- Coffee/tea in a common area
- Fitness facilities
- Spa services on site
- One meeting room
- 24-hour front desk
- Concierge services
- Tours/ticket assistance
- Limo or Town Car service available
- Dry cleaning/laundry service
- Free newspapers in lobby
- Luggage storage
- Multilingual staff
- Number of buildings/towers - 1
- Lift
- Safe-deposit box at front desk
- Television in common areas
- Croatian
- English
- Filipino
- German
- Hungarian
- Italian
- Polish
- Romanian
- Russian
- Serbian
- Spanish
In the room
- In-room climate control (air conditioning)
- Minibar
- Hypo-allergenic bedding available
- Blackout drapes/curtains
- Premium bedding
- Number of living rooms 1
- Separate sitting area
- Private bathroom
- Bathtub or shower
- Free toiletries
- Hair dryer
- Desk
- Free WiFi
- Phone
- Daily housekeeping
- In-room safe
Recommended hotels
Starlight Suiten Hotel Renngasse's small print
Also known as
- Starlight Suiten Hotel Renngasse Wien Vienna
- Starlight Wien Renngasse
- Starlight Suiten Ii Renngasse Hotel Vienna
- Starlight Suiten Ii Renngasse Vienna
- Starlight Suiten Hotel Renngasse Vienna
- Starlight Suiten Renngasse Vienna
- Starlight Suiten Renngasse
- Starlight Suiten Hotel Renngasse Hotel
- Starlight Suiten Hotel Renngasse Vienna
- Starlight Suiten Hotel Renngasse Hotel Vienna
- Starlight Suiten Hotel Renngasse Wien
- Starlight Suiten Renngasse Wien Vienna
- Starlight Suiten Renngasse Wien
- Starlight Suiten Ii Renngasse Hotel Vienna
- Starlight Suiten Hotel Renngasse Vienna
- Starlight Suiten Renngasse Vienna
- Starlight Suiten Renngasse
- Starlight Wien Hotel
Only registered guests are allowed in the guestrooms.Guests can rest easy knowing there's a carbon monoxide detector, a fire extinguisher, a smoke detector, a security system, a first aid kit, and window guards on site.
This property advises that enhanced cleaning and guest safety measures are currently in place.
Disinfectant is used to clean the property; commonly-touched surfaces are cleaned with disinfectant between stays; bed sheets and towels are laundered at a temperature of at least 60°C/140°F.
Personal protective equipment, including masks and gloves, will be available to guests.
Social distancing measures are in place; staff at the property wear personal protective equipment; a shield is in place between staff and guests in main contact areas; periodic temperature checks are conducted on staff; guests are provided with hand sanitizer.
Individually-wrapped food options are available for breakfast.
Each guestroom is kept vacant for a minimum of 24 hours between bookings.
The property affirms that it adheres to the Intertek Cristal (3rd party expert - Global) sanitization guidelines and the Safe Travels (WTTC - Global) sanitization guidelines.
For transfers, guests must contact the property 72 hours prior to arrival at the number on the booking confirmation.
Optional extras
Self parking costs EUR 32 per day with in/out privileges
Rollaway beds are available for EUR 30.0 per night
Buffet breakfast is offered for an extra charge of EUR 18 per person (approximately)
Cribs/Infant beds are available for EUR 0 per night
Pets are allowed for an extra charge of EUR 15 per accommodation, per night
Airport shuttle service is offered for an extra charge of EUR 47 per vehicle (one way)
Area shuttle service is offered for an extra charge
We have included all charges provided to us by this property. However, charges can vary, for example, based on length of stay or the unit you book.
FAQs for Starlight Suiten Hotel Renngasse
Does Starlight Suiten Hotel Renngasse offer free cancellation for a full refund? Yes, Starlight Suiten Hotel Renngasse does have fully refundable rooms available to book on our site, which can be cancelled up to a few days before check-in. Just make sure to check this property's cancellation policy for the exact terms and conditions. Is parking offered at Starlight Suiten Hotel Renngasse? Yes. Self parking costs EUR 32 per day. Are pets allowed at Starlight Suiten Hotel Renngasse? Yes, pets are welcome, with a limit of 2 per room. There's a charge of EUR 15 per accommodation, per night. What are the check-in and check-out times at Starlight Suiten Hotel Renngasse? You can check in from 3:00 PM to midnight. Check-out time is noon. Is airport shuttle service offered by Starlight Suiten Hotel Renngasse? Yes, an airport shuttle is available. The charge is EUR 47 per vehicle one way.
Recent reviews
Very good 8.4 From 280 reviews
This is a dated apartment style accommodation that is much in need of a make-over. I specifically booked accommodation with air-conditioning yet upon arrival quickly discovered the hot room had no working cooling coming at all. Whilst the staff provided a fan, it made for an uncomfortable and disappointing experience.
The property has good location ,10 minutes by walking to city center. The suite room for 3 people has one double bed and another bed which is not comfortable at all. The sofa in salon was with holes. The view from the window room is to internal wall. The service staff is pleasent to guests.the cleaning is just ok.
We had a very lovely and comfortable stay at the hotel. The staff at the front desk were helpful and welcoming. The room was very clean, spacious and well decorated. I definitely will return to it should I visit Vienna again.
Great location. Room was spacious and clean. Comfortable bed.
Big rooms, comfortable beds, good location. Nice for family with kids. Really good buffet.
Really good location, clean, safe, fun, everything unless you need Internet for work. The Internet is as good as Fiji, Chad and the French countryside - basically 3rd world. If you're coming on vacation this is the place for you - leave the computer behind as it won't connect!
Lovely staff, large room to accommodate our family, nice area within walking distance to many sites.
5 day stay for business. The location and room were great but it was not possible to control the temperature satisfactorily as the room remained too warm (in February/March!) and I had to sleep with the windows open. The crockery in the room was not regularly replaced with clean crockery and had to be washed before use. I did mention this to the desk and they took it on board.
We came for the christmas markets for the third time and this hotel was great. We have stayed at higher rated hotels in the past but after seeing the room sizes and location we wanted to try this one. Staff was great, even helped arrange a doctor to see my father who got sick. Couldn't have asked for a better spot especially for the value. We highly recommend this hotel and we will be back! Rooms were great for a family given the size and they had all the things you need. There are two christmas markets within 6 minutes of the hotel and plenty of dining options too.
Location was very good for Christmas Markets,room was very spacious and clean,buffet breakfast was good with a good choice.
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This tour has been prepared for the whole people who wants to enjoy interacting with diffrents cultures. Our company possesses (relies on) everything the necessary equipment to satisfy this adventure. You will know beautiful destinations such as the city of Lima with its tourist attractions such as the Arzobispado de Lima, the Cathedral of Lima, the Plaza San Martin, the Palace of Justice among others.
The Ballestas Islands will give you a beautiful picture when they enjoy a whole day in the natural habitat of sea lions and guaneras birds that show that in Peru these species can live in harmony with humans. In addition we will know the mysterious Nazca lines, you will have the pleasure of flying over them and delighting yourself with the beautiful view of the pampas where the gigantic Nazca lines are drawn. We will advance further south in the direction of the white city of Arequipa where we will take a break, then go on to an experiential experience in the Uros Islands, we will continue in the direction of the imperial city, Cusco.
This city awaits us with gratitude and with various attractions to which we can access and enrich ourselves with the culture of this region of the country, for having been in ancient times the capital of the Tahuantinsuyo Empire. We will meet the living community of Willoq, a community a few kilometers from the Ollantaytambo train station, which will take us to enjoy a beautiful day in the majestic and impressive Lost city of the Inca and Modern Wonder of the World, Machupicchu.
Without a doubt, this tour will leave you in love with the culture, traditions and customs of Peru as it contains the most beautiful tourist destinations in the country.
Transfer in Lima
Free day in Lima.
Full day excursion in the Ballestas Islands
Overflight of the Nazca lines
Free day in Arequipa.
Transfer out Arequipa.
Transfer in Puno.
Time in Puno to acclimatize.
Excursion to the floating islands Uros.
Overnight at Uros Lodge.
Transfer out Puno and Transfer in Cusco.
City tour Cusco + 4 archaeological parks.
Visit to the Willoq community.
Excursion to the citadel of Machu Picchu.
Return to the city of Cusco
Transfer out Cusco.
Transfer to the airport.
Observations for the trip:
Accommodation: Hotel in Lima.
Places: Lima
Highest Elevation: 161 masl / 528 feet ASL
Welcome to Peru! Being the only capital city of America on the seafront, Lima is populous (more than 8 million inhabitants) and full of world-famous attractions and restaurants. It is the gastronomic capital of America and only for that reason, one of the many that it has, becomes a pleasant tourist center. Its beaches also offer us the beauty of its women and its waves. When you arrive to the hospital.
We will continue our tour to the Parque del Olivar and the Financial Center then we will go to the Historic Center of Lima, declared by UNESCO in 1991 Cultural Heritage of Humanity, where the center of the Spanish colony was established in the 16th century and they were erected the first buildings. We will know the Plaza San Martín, its balconies and old houses, the Plaza Mayor and its most important buildings such as the Government Palace, the Cathedral of Lima, the Archbishop and Municipal Palace, the Station of Desamparados, among others
We will enter one of the most important convents of the 17th century. Where the relics of the three Peruvian saints Santa Rosa de Lima, San Martin de Porres and San Juan Macías are preserved. At the end of our tour you will have the option to stay in the Indian market, in Larcomar or return to your hotel.
Accommodation: Hotel in Lima.
Places: Lima
Highest Elevation: 161 masl / 528 feet ASL
This day you will have enough time to carry out activities on your own.
It is necessary that you acclimatize since in the following days we will pass through different cities with different climates.
You can visit other attractions on your own, you can make availability inquiries and more.
Accommodation: Hotel in Nazca.
Places: Paracas, Ica.
Highest Elevation (Ica): 406 masl / 1,332.02 feet ASL
Very early in the morning between 2:50 am -3: 00 am, you will be picked up from the hotel to be taken to the bus station and transferred to the bus station. After picking up our passengers from Lima to the Paracas in Cruz del Sur. We will arrive at the city of Paracas, we will be received for a specialized staff and transferred to the tourist pier. Our tour will start at 08:00 am to the Ballestas Islands, while we sail the Pacific Ocean we will appreciate a gigantic and peculiar figure that resembles a chandelier. It is an enigmatic figure that exists in the dunes facing the sea. Then we will continue the tour and we will be able to observe different sea birds that produce the guano of the islands, we will appreciate the ecosystem of the Ballestas Islands, we will see Humbolt Penguins and the sea lions in their natural habitat. After enjoying this beautiful landscape we will return to the Port.
Accommodation: Hotel in Arequipa.
Places:Ica, Nazca, Nazca aerodrome, from Arequipa.
Highest elevation (Arequipa) : 2,335 meters ASL / 7,660 feet ASL
Early in the morning we will pick you up from the hotel and leave for the aerodrome where we will board a light plane for a memorable air excursion over the Nazca Lines. A bilingual guide (Spanish / English) will assist you at all times until you board the plane.
This overflight over the Nazca Lines will cover the extension of the Nazca desert stone, whose surface is marked by gigantic drawings and geometric figures.
The overflight to the Nazca lines lasts approximately 35 minutes and is the best way to see these fascinating lines and drawings that have intrigued people for years. Here, you will be able to see the different figures traced on the stone surface of the Nazca desert, representing stylized animals and plants, distributed in a huge maze of lines, trapezoids, triangles and spirals that have been preserved for almost 2000 years. It is one of the most incredible legacies that a culture has left behind and that can only be seen from the sky at an altitude of 1500 feet.
After the overflight we will have time to have lunch and take a bus to Arequipa at 02:30 pm.
We will be assisted upon arrival in Arequipa and transferred to the hotel where we will spend the night.
Places: Arequipa.
Accommodation: Hotel in Arequipa
The Highest Elevation: 2,335 masl / 7,661 feet ASL
After breakfast, you will have the day off for the opportunity to go out on the town.
You can choose to do activities of your choice or walk around the city a little, taste its gastronomy or do some shopping, this day is yours, take advantage of it to enjoy it on your own and thus be able to acclimatize yourself better.
Meals: Breakfast
Accomodation: Hotel in Puno
Places: Arequipa, Puno
This morning after breakfast we have time to walk and acclimatize or make a personal activities, but since at approximately 12:30 pm, we will board the bus to move to the city of Puno. Let's take advantage of buying water to hydrate ourselves a little. At 19:30 pm approximately we will arrive to the city of Puno, We will receive you at the bus station and will be transferred to the hotel. Today our overnight will be in Puno.
Meals: Breakfast in a hotel in Puno, lunch and dinner in the Uros.
Accommodation: Lodge in the Uros.
After taking our breakfast in the morning. Our experience begins with the transfer from your hotel to the populated center of Uros-Chulluni where we will embark with destination to the floating islands of the Uros received by a local family. Pleasant place to sleep in Uros, free of pollution, acoustic noise and relaxing located 30 minutes from the city of Puno.The rooms are builtas that the island made of cattails with a view of the lake, firm wooden floor and decorated and implemented with fabrics and crafts, to sleep in Uros with comfortable mattresses, cushions decorated with textiles. Our native rural accommodation to sleep in Uros has all you need to rest in any accomodation, they take us to their rural family accommodation in their traditional boats 15 minutes from the indicated port, then we will settle in the properly equipped rooms so that we can rest, once installed we will taste a delicious lunch offered by the family, then we can appreciate its different activities in the Uros, such as artisanal fishing, Corte de la Totora, its Dress in typical native costumes, kayak in Totora boats and visits to the surrounding communities. Guests can also learn traditional crafts and weaving techniques.
Meals: Breakfast
Accommodation: Hotel in Cusco
Elevation:3,999 masl / 11,151 ft ASL
In the morning, we will wake up in the Uros islands, we will taste an exquisite breakfast. Then at the appropriate time, we will transfer you to the city of Puno. Upon arrival at the bus station to move to the city of Cusco. We will be able to observe beautiful landscapes, little by little we will be leaving the city of Puno and entering the city of Cusco.
Meals: Breakfast.
Accommodation: Hotel in Cusco.
Places: Cusco, Plaza de Armas, Qoricancha, Sacsayhuaman, Qenqo, Puca Pucara, Tambomachay.
The Highest Elevation (Sacsayhuaman) : 3,700 masl / 12,139 feet ASL
You will wake up in the beautiful city of the Incas, Cusco. Depending on the season, keep yourself warm. After breakfast, you will have the morning free to be able to walk a little through the beautiful streets of the city center on your own, so that you can carry out your personal activities and get acclimatized a little.
The tour begins with the pick up of our travelers from their respective hotels, panoramic visit of the Plaza de Armas and the Cathedral. We will also visit the Inca Temple of the Qoricancha, the maximum expression of Inca architecture and engineering. Then you will visit the four nearby ruins of the city located 15 minutes away: Sacsayhuamán, Qenqo, Puca Pucara and Tambomachay at the end of the return to the hotel.
Meals: Breakfast lunch.
Accommodation: Hotel in Aguas Calientes.
Places: Cusco, Willoq, Ollantaytambo.
Willoq's Elevation: 3,754 masl / 12,316 feet ASL
The tour begins at 06:00 in Cusco and ends at 7:00 p.m. (full day) in Willoq, then We will head towards to Aguas Calientes.
We will pick you up at your hotel in Cusco at 6:00 am in the direction of Ollantaytambo (living Inca city), there we will make a brief stop to visit the city, then we will go to the native community of Willoq located 14 km from Ollantaytambo, the town of Willoq, is an indigenous community of the Ande, located in the valley of the Patacancha river, belonging to the Ollantaytambo district, Urubamba province, in Cusco.
It is rich in flora and fauna, being able to find 60 species of birds and more than 700 species of plants. In Willoq there is still a direct descent from the last Incas that can be corroborated by the current surnames of the local people, such as Yupanqui, Sinchi, Pukha, Huamán, Cusipáucar, Tupha, among others. Once in Willoq our local friends will welcome us with a welcome drink (mate de coca or muña) and their folk dance, they will immediately show us and share their customs and traditions and daily activities (Meet, interact, share the customs and traditions Incas of this Community, plant, sew, cook and have fun with their typical dances. The approximate duration of the activity is about 3 to 4 hours, then we will have a typical lunch of the place and after a break. We will move to the Ollantaytambo train station. We will take the train service that will take us to the beautiful town of Aguas Calientes, where we can rest at the hotel. So that the next day we go to the majestic citadel of Machupicchu.
Meals: Breakfast, lunch.
Accommodation: Hotel in Cusco.
Places: Cusco, Ollantaytambo, Machupicchu.
Machupicchu Elevation: 2430 masl / 7972 ft ASL
At a fixed time, we will transfer to the bus station, to board the bus from Machu Picchu town to Machu Picchu the Inca City. We will have a visit in the city with a professional bilingual guide, after 02 hours, we will be able to visit the Inca city on our own. You will able to take pictures of your fascinating journey.
At the appropriate time we will return to the town of Aguas Calientes to enjoy a delicious buffet lunch.
Then we will transfer to the train station to return to Ollantaytambo and then to Cusco.
We will take you to the airport at the scheduled time so that you can board your plane to the city of Lima. Where you must wait for your international flight to return home. We hope that your visit to Peru is a reason to return. We will be happy to take you through multiple experiences.
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Destiny: Cusco - Perú
William Escalante Taype
Cellphone: +51-084-253617
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Name: Inka Trail Expeditions Peru
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Adress: San Agustin Street 317
City: Cusco - Perú
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Cyclin-dependent kinase inhibitor p27Kip1 functions at the nuclear level by binding to cyclin E/cyclin-dependent kinase-2. It was shown that Akt or protein kinase B (Akt/PKB)-dependent phosphorylation of p27Kip1 led to the cytoplasmic mislocalization of p27Kip1, suggesting the potential abrogation of its activity. Here, we evaluated the localization of p27Kip1 protein in leukemic blasts in relation to Akt/PKB phosphorylation and clinical outcomes in acute myelogenous leukemia (AML). Western blot analysis of the nuclear and cytoplasmic fractions revealed a heterogenous localization pattern of p27Kip1 in AML. Cytoplasmic mislocalization of p27Kip1 was significantly associated with the constitutive serine473 Akt/PKB phosphorylation in AML cells (P < 0.05). Transfection of U937 cells with an expression construct encoding the constitutively active form of Akt/PKB resulted in a remarkable increase in the levels of cytoplasmic p27Kip1. Whereas the transfection of U937 cells with a construct encoding dominant-negative Akt/PKB resulted in a recovery of nuclear localization of p27Kip1. Both the disease-free survival and overall survival are significantly shorter in AML cases with high cytoplasmic to nuclear ratio of p27Kip1 localization compared with the cases with low cytoplasmic to nuclear ratio (P = 0.0353, P = 0.0023, respectively). Multivariate analysis indicated that the cytoplasmic to nuclear ratio of p27Kip1 localization was an independent prognostic variable for both disease-free survival and overall survival (P = 0.043, P = 0.008, respectively). These findings additionally extend our understanding of the role of p27Kip1 in AML, and buttress the case of p27Kip1 mislocalization as a prognostic indicator and Akt/PKB/p27Kip1 pathway as a ready target for antileukemia therapy.
The cyclin-dependent kinase (CDK) inhibitor p27Kip1 is an important regulator of G1 progression and negatively regulates cell proliferation. It is expressed highly in G0, where it binds tightly and inhibits cyclin E/CDK-2 (1, 2, 3, 4) . The p27Kip1 protein levels are regulated predominantly by ubiquitin/proteasome-mediated proteolysis (4 , 5) in a process that requires its association with cyclin/CDK complexes (6 , 7) and phosphorylation of the threonine (Thr)187 of the p27Kip1 protein (6 , 8 , 9) . The expression of S-phase kinase-associated protein 2, a member of the F-box family of the specific substrate-recognition subunit of Skp1/Cull/F-box (SCF) ubiquitin-protein ligase complexes (10) is required for the ubiquitination and subsequent degradation of p27Kip1 (11, 12, 13, 14, 15) .
Although p27Kip1 is rarely mutated in human cancers, there are considerable evidences that the inactivation of p27Kip1 is a fundamental step for the development of malignancies (16, 17, 18, 19) . The reduced expression of p27Kip1, because of increased protein degradation, correlates with the poor prognosis of patients with various types of cancer (16 , 17 , 19, 20, 21, 22, 23) . Paradoxically, some tumors may contain elevated levels of p27Kip1 protein (17 , 20 , 23) , suggesting that they have developed other mechanisms to circumvent p27Kip1 growth inhibition. High levels of p27Kip1 might inhibit the efficacy of chemotherapeutic agents, which rely on the interfering with the cell proliferation to trigger a cytotoxic response (18 , 24) . This opposing effect of p27Kip1 as a prognostic relevance prompts additional efforts to understand the mechanisms involved in the modulation of the p27Kip1 activity and its consequences on the therapeutic outcomes in specific tumors.
In human tumors, p27Kip1 protein itself, or its activity, appears to be lost by increased cytoplasmic mislocalization or sequestration as well as increased degradation (25) . In tumors with abundant p27Kip1 expression, the protein is often mislocalized to the cytoplasm (26, 27, 28) . Because the growth-restraining activity of p27Kip1 depends on its nuclear localization, the cytoplasmic mislocalization can effectively inactivate the p27Kip1 inhibitory activity (26 , 29, 30, 31, 32) . Cytoplasmic p27Kip1 appears to directly correlate with a poor prognosis and advanced tumor grade of esophagus and breast carcinoma (28 , 33 , 34) . These findings suggest that the elucidation of the mechanisms implicated in the regulation of p27Kip1 mislocalization can provide the insight into the aberrant p27Kip1 regulatory pathway and its role in tumor cells.
Akt or protein kinase B (Akt/PKB) is a central component of the phosphatidylinositol 3′-kinase pathway (35, 36, 37) , a pathway replete with oncogenetically relevant molecules (38) . Akt/PKB can down-regulate the p27Kip1 by increasing its proteolysis (39) or repressing its expression through Akt/PKB-mediated phosphorylation of the forkhead transcription factor (40) . Recently, a novel mechanism of Akt/PKB-mediated p27Kip1 regulation was demonstrated (34 , 41) . The p27Kip1 is phosphorylated at Thr157 by Akt/PKB both in vitro and in vivo (33 , 41) . This Akt/PKB-dependent phosphorylation results in an impairing of nuclear import of p27Kip1 protein, leading to its cytoplasmic mislocalization and abrogation of its cyclin E/CDK2 inhibitory activity (33 , 41) . The Akt/PKB phosphorylation has been shown to correlate statistically with the cytosolic mislocalization of p27Kip1 in tumor cells (33 , 41) . These data indicate that Akt/PKB activation may contribute to the tumor-cell proliferation via phosphorylation and cytoplasmic retention of p27Kip1, thus, relieving CDK2 from the p27Kip1-induced inhibition.
We demonstrated previously that the constitutive phosphorylation of Akt/PKB was observed in a substantial proportion of acute myelogenous leukemia (AML) and associated with a poor prognosis (42) . However, the cytoplasmic mislocalization of p27Kip1 protein in relation to Akt/PKB phosphorylation and clinical outcomes has not been evaluated in AML. In this study, the constitutive phosphorylation of Akt/PKB was correlated highly with the cytoplasmic mislocalization of p27Kip1 protein in AML. The induced overexpression of Akt/PKB activity in leukemia cells resulted in a markedly increased localization of p27Kip1 to the cytoplasm. The pronounced cytoplasmic mislocalization of p27Kip1 was significantly associated with the reduced survival in AML and remained an independent prognostic factor in a multivariate analysis. These findings additionally extend our understanding of the role of p27Kip1 in AML and support the case of p27Kip1 mislocalization as a prognostic indicator and the Akt/PKB/p27Kip1 pathway as a ready target for antileukemia therapy.
Patients and Treatment.
A total of 99 consecutive adults with de novo AML who had not received treatment were included in the study. According to the French-American-British classification, 4 patients had M0, 20 M1, 33 M2, 19 M4, 21 M5, and 2 M6 subtype. Patients with AML (M3) were excluded from the study. Eighty patients received induction chemotherapy, comprising of cytarabine (100 mg/m2/d by continuous infusion for 7 days) and idarubicin (12 mg/m2/d IV bolus for 3 days). Complete remission was defined as the normalization of blood counts and bone marrow morphology and the disappearance of all signs of leukemia, for at least 4 weeks or longer, in accordance with the recommendations of the National Cancer Institute-sponsored Workshop (43) . All of the patients achieving complete remission then received the same two courses of consolidation chemotherapy consisting of cytarabine (1g/m2/d IV infusion for 2 h every 12 h for 4 days), mitoxantrone (12 mg/m2/d for 3 days), and VP-16 (100 mg/m2/d for 2 days) as described previously (44) .
Isolation of Leukemic Cells.
In conjunction with the institutional review board-approved treatment protocol, bone marrow aspirates were prepared prospectively from the patients before the initiation of chemotherapy. Marrows were sedimented on a Ficoll-Hypaque (Pharmacia Biotech, Uppsala, Sweden) density gradient. After washing the mononuclear cells collected from the upper interface, T-cell depletion was performed using a high-gradient magnetic cell separation system/anti-CD3 monoclonal antibody (Miltenyi Biotech, Auburn, CA) according to the manufacturer's instructions. A morphological evaluation indicated that >95% of the isolated cells were leukemic blasts.
Cell Line.
The U937 human leukemia cell line was purchased from the American Type Culture Collection (Manassas, VA). The cells were grown in RPMI 1640 (Life Technologies, Inc., Rockville, MD) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone Laboratories, Logan, UT), 1% penicillin/streptomycin, sodium pyruvate, and 2 mm l-glutamine (Life Technologies, Inc.).
Antibodies and Reagents.
The rabbit polyclonal antibodies against total Akt/PKB and phospho-serine (Ser)473 Akt/PKB were purchased from Cell Signaling Technology (Beverly, MA). The mouse monoclonal antibody against p27Kip1 was obtained from Zymed (San Francisco, CA). The rabbit polyclonal antibody against p27Kip1, HRP-conjugated goat-antimouse IgG and HRP-conjugated goat-antirabbit IgG were obtained from Transduction Laboratories (Lexington, KY). The antihuman α-tubulin monoclonal antibody was from Cedarlane (Ontario, Canada). The protein A-agarose bead was from Upstate Biotechnologies (Lake Placid, NY). All of the other culture reagents were purchased from Life Technologies, Inc. unless indicated otherwise.
Cytogenetic Analysis.
Cytogenetic G-banding analysis was performed on pretreated bone marrow cells. The definition of the cytogenetic clone and descriptions of karyotypes followed the International System for Human Cytogenetic Nomenclature. Patients were divided into three prognostic groups based on their karyotype: (a) prognostic groups were favorable [t(8;21), inv (16)] ; (b) intermediate (normal cytogenetics); and (c) unfavorable (all other abnormalities).
Cell Cycle Analysis.
Leukemic cells were pelleted, fixed in 70% ethanol on ice for 1 h, and resuspended in 1 ml of cell cycle buffer (0.38 mm sodium citrate, 0.5 mg/ml RNase A, and 0.01 mg/ml propidium iodide) at a concentration of 106 cells/ml. Cell cycle analysis was carried out using a FACSCalibur flow cytometer equipped with CellQuest software (Becton Dickinson, San Jose, CA).
Transient Transfection.
Cells were plated in 12-well plates containing RPMI 1640 supplemented with 10% (v/v) fetal bovine serum and 1% penicillin/streptomycin at a density of 2 × 106 cells/well. The next day, a transfection mixture was prepared by mixing 5 μg of DNA (expression vectors) with 100 μl of human Nucleofector solution (Amaxa Biosystems, Gaithersburg, MD), which was then used to transfect 2 × 106 leukemia cells. Cells were transfected with expression constructs encoding the constitutively active form of the Akt/PKB (pcDNA3-myristoylated-Akt/PKB) or dominant negative (DN)-Akt/PKB (pcDNA3-DN-Akt/PKB), which were kindly provided by Dr. Young-Guen Kwon (Yonsei University, Seoul, Korea). The cell suspension was immediately electroporated by a Nucleofector instrument (Amaxa Biosystems), according to the manufacturer's instructions. Immediately after electroporation, the cells were suspended in the complete medium and incubated in a humidified 37°C/5% CO2 incubator. The cells were harvested 48 h after transfection and used for the experiments. The experiments were repeated at least three times.
Confocal Microscopy and Image Analysis.
Confocal microscopy was used to examine the localization of the p27Kip1 protein in AML cells. Cells were fixed, permeabilized, and blocked with 2% BSA. Cells were incubated with the primary antibody against p27Kip1 (1:100 dilution) for 1 h at 37°C and then incubated with the corresponding FITC-conjugated secondary antibody (1:200 dilution). After the cells had been spun down on a slide, the nuclei were counterstained by 4′,6-diamidino-2-phenylindole (Molecular Probes, Eugene, OR) for 1 min. After washing, the slides were mounted in a drop of antifade mounting medium (Vectashield; Vector Laboratories, Burlingame, CA). Antibody labeling was examined by a Bio-Rad 1024-UV confocal system attached to a laser scanning confocal microscope (Leica Lasertechnick GmbH, Heidelberg, Germany). Control experiments, with the omission of the primary antibody, showed negative staining in all of the experiments. Simultaneous FITC or 4′,6-diamidino-2-phenylindole images were captured from the same optical section. The captured images were then pseudocolored as follows: (a) blue for 4′,6-diamidino-2-phenylindole; and (b) green for FITC. Regions of colocalization appear in cyan, reflecting the additive effect of superimposing the green and blue pixels. Image analysis was performed using the standard system operating software provided with the confocal microscope. All of the illustrations were assembled and processed digitally (Adobe PhotoShop, version 6.0; Adobe Systems Inc., San Diego, CA).
Preparation of Nuclear and Cytoplasmic Fractions.
Cells were subfractionated, as described previously (45) , with minor modifications. Briefly, cells were pelleted by centrifugation (5 min, 12,000 rpm, 4°C) and incubated in a hypotonic buffer [10 mm HEPES (pH 7.2), 10 mm KCl, 1.5 mm MgCl2, 0.1 mm EGTA, 20 mm NaF, 100 μm Na3VO4, and a 0.1% protease inhibitor cocktail; Sigma Chemical, St. Louis, MO] for 30 min at 4°C, with rocking. Cells were broken using a Dounce homogenizer (30 strokes), after which the nuclei were pelleted by centrifugation (10 min, 3,500 rpm, 4°C). The nuclei-free supernatant was subjected to a second 10,000 × g centrifugation for 45 min at 4°C to separate the membranes from the cytosolic fractions. The nuclear pellets were resuspended in nuclear lysis buffer [10 nm Tris-HCl (pH 7.5), 150 mm NaCl, 5 mm EDTA, and 1% Triton X-100], incubated for 1 min in a sonicating water bath, then incubated for 30 min at 4°C, with rocking. Twenty μg of total cytosolic and nuclear proteins were analyzed by Western blotting.
Western Blotting.
The cells were dissolved in 100 μl of SDS-PAGE sample buffer containing β-mercaptoethanol to a final concentration of 3 × 106 cells. Lysates were sonicated for 15 s with a Vibra Cell Sonicator, boiled for 10 min, and additionally analyzed by Western blotting. The protein yields were quantified using the Bio-Rad Dc protein assay kit (Bio-Rad, Hercules, CA) and equivalent amounts of protein applied to 15% acrylamide gels. The proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Amersham Biosciences, Sunnyvale, CA). The membranes were blocked with 3% BSA in Tris-buffered saline-Tween (TBST; 1 × Tris-buffered saline, 0.1% Tween 20) for 2 h. After washing twice in TBST, the membranes were incubated with the primary antibodies for 2 h at room temperature. The membranes were then washed four times in TBST and incubated with the relevant horseradish peroxidase-conjugated secondary antibodies (1:3000 dilution with 3% BSA in TBST) for 30 min. After washing four times in TBST, the reactive proteins were visualized with an enhanced chemiluminescence detection system (Amersham Biosciences). Densitometry was performed by the Molecular Dynamics Imaging system and ImageQuant 3.3 software (Amersham Biosciences) to quantify relative amounts of protein detected on the Western blots.
Statistical Analysis.
The patients were divided into two groups (high and low) in relation to the median value of the cytoplasmic to nuclear ratio (C:N) of the p27Kip1 protein localization. Comparisons among the characteristics of the groups were made using a chi-square test for the binary variables and a Mann-Whitney test for the continuous variables. The disease-free survival (DFS) and overall survival (OS) probabilities were calculated using the Kaplan-Meier method. The log-rank statistic was used to test for the difference in survival times between the groups. In addition to the p27Kip1 protein localization, the WBC count, age, and cytogenetics were analyzed in the univariate and multivariate analysis. A multivariate analysis was used to test for the independent prognostic significance of the variables using the Cox proportional hazards regression model. Patients alive and still in remission at their last follow-up examination were censored in the analysis. A P < 0.05 was used to indicate statistical significance. All of the calculations were performed using the SPSS software, version 11.0.1 (SPSS Inc., Chicago, IL).
The p27Kip1 Protein Expression in AML Cells.
Western blot analysis demonstrated that the p27Kip1 protein was expressed to a variable degree in 88 (88.9%) of 99 AML cases (Fig. 1A) ⇓ . To perform the quantitative analysis, the expression level of p27Kip1 protein was evaluated in the AML cells (L) by normalization as follows: Lc = p27Kip1 (L)/α-tubulin (L) as determined by Western blot analysis. In this study, the Lc ranged from 0 to 68.3 (median, 8.08). There was no association between the cellular levels of the p27Kip1 protein and the various clinical parameters such as age, French-American-British classification, WBC count, lactic dehydrogenase level, cell-cycle distribution, cytogenetics, complete remission rate, DFS, and OS (data not shown).
Subcellular Localization of p27Kip1 Protein in AML Cells.
Next, the subcellular localization of the p27Kip1 protein was examined in the leukemia cells obtained from 88 AML patients. Western blot analysis of the fractionated cell lysates demonstrated that the subcellular localization of the p27Kip1 protein was different, according to the patients (Fig. 1B) ⇓ . To perform the quantitative analysis, the relative levels of the p27Kip1 expression at the subcellular compartment were represented as the C:N ratio of the p27Kip1 protein levels as shown in the following data: C:N = p27Kip1(C)/p27Kip1(N) as determined by the Western blot analysis. In this study, the C:N ratios ranged from 0 to 127.21 (median, 1.97). For the practical evaluations, the median value was used as a cutoff value for the levels of subcellular localization of the p27Kip1 in the AML cells. Using this value, the AML cases were classified into two groups; the high and low C:N ratio groups (n = 44 for both; Table 1 ⇓ ). Next, the association between the C:N ratio of the p27Kip1 localization and various clinical parameters in AML were analyzed. As shown in Table 1 ⇓ , the C:N ratio of the p27Kip1 localization was not correlated with age, gender, WBC count, lactic dehydrogenase level, French-American-British classification, or cytogenetics. Cell cycle analysis demonstrated that the fraction of cells in the G0/G1 phase were not different between the AML cells, showing either a high or low C:N ratio of the p27Kip1 localization (86.4 ± 11.9 and 79.4 ± 27.0%, respectively; Table 2 ⇓ ). Likewise, the C:N ratio of the p27Kip1 localization was not correlated with the fractions of the cells in the G2-M or S-phase (Table 2) ⇓ .
Correlation between Constitutive Akt/PKB Phosphorylation and p27Kip1 Localization.
The constitutive phosphorylation of Ser473 Akt/PKB was demonstrated in 58 (58.6%) of 99 cases (Fig. 2A) ⇓ . Cell cycle analysis showed that neither the fraction of cells in the G0/G1 phase, G2-M phase, or S phase were different, according to the phosphorylation of Akt/PKB (data not shown). Next, the association of the phosphorylation of Akt/PKB and localization of the p27Kip1 protein was examined in the AML cells. Akt/PKB phosphorylation was observed more frequently in the high C:N ratio group compared with the low C:N ratio group (77.3% versus 54.5%, P = 0.042; Fig. 2B ⇓ ). The mean value of the C:N ratio of the p27Kip1 localization was significantly higher in the pAkt/PKB-positive AML cases compared with the pAkt/PKB-negative AML cases (18.15 ± 4.2 versus 9.2 ± 3.3, P = 0.043; Fig. 2A ⇓ ). The confocal microscopic analysis revealed the p27Kip1 protein to be preferentially localized to the cytoplasm in a pAkt/PKB-positive AML case (Fig. 3A) ⇓ . In contrast, the nuclear staining of the p27Kip1 protein was evident in a pAkt/PKB-negative AML case (Fig. 3B) ⇓ .
The p27Kip1 Mislocalization Induced by Akt/PKB Overexpression in Leukemia Cells.
To investigate whether the active Akt/PKB directly affects the localization of the p27Kip1 protein in leukemia cells, the U937 leukemia cells were transfected using the pcDNA3-myristoylated-Akt/PKB or pcDNA3-DN-Akt/PKB expression vectors (Fig. 4A) ⇓ . As shown in Fig. 4B ⇓ , the expression of the p27Kip1 protein was preferentially localized to the cytoplasm of the myristoylated-Akt/PKB-transfected U937 cells. In contrast, the DN-Akt/PKB-transfected U937 cells revealed a reduced level of the p27Kip1 in the cytoplasm, but increased the p27Kip1 levels in the nuclei.
The p27Kip1 Localization as a Prognostic Variable in AML.
The complete remission rate of the patients with the high C:N ratio of the p27Kip1 localization was lower than that with the low C:N ratio (75% versus 95%), but without statistical significance (P = 0.116; Table 1 ⇓ ). A survival analysis using the Kaplan-Meier method demonstrated that the high C:N ratio group had a significantly shorter DFS than the low C:N ratio group (Fig. 5A ⇓ ; P = 0.0353 by log-rank test). The OS rate was also significantly lower in the high C:N ratio group compared with the low C:N ratio group (Fig. 5B ⇓ ; P = 0.0023). The DFS estimates at 5 years for patients with a high or low C:N ratio of p27Kip1 localization were 19.0 (SE = 14.6%) and 58.2% (SE = 12.4%), respectively (P = 0.0353). The OS estimates at 5 years for patients with a high or low C:N ratio were 10.8 (SE = 8.4%) and 52.6% (SE = 11.2%), respectively (P = 0.0023). The univariate analysis revealed that the C:N ratio of the p27Kip1 localization was a strong prognostic factor both of the DFS and OS (Table 3) ⇓ . A multivariate analysis of the covariates in the Cox regression model demonstrated that the C:N ratio of the p27Kip1 protein localization remained as an independent prognostic factor of the DFS [relative risk (95% confidence interval) = 2.326 (0.989–5.466), P = 0.043] and the OS [relative risk (95% confidence interval) = 2.399 (1.255–4.585), P = 0.008] (Table 4) ⇓ .
In this study, the cytoplasmic mislocalization of the p27Kip1 protein was demonstrated in a substantial proportion of the AML cases. The p27Kip1 mislocalization to the cytoplasm was highly correlated with the constitutive phosphorylation of Akt/PKB in the leukemic cells and with a poor prognosis in AML. However, the mere cellular levels of the p27Kip1 protein were not correlated with the clinical outcome. This study supports a growing body of evidences suggesting that the cytoplasmic p27Kip1 may play a role as an oncoprotein with antiapoptotic properties (26, 27, 28 ,, 46) .
Much less is known about the localization of p27Kip1 protein during the cell cycle progression. To inhibit cyclin E/CDK2, p27Kip1 needs to be imported into the nucleus (3) . As cells progress along the cell cycle, the p27Kip1 protein shuttles between the nucleus and cytoplasm (47) . The cytoplasmic redistribution of p27Kip1 induced by mitogenic stimulation is dependent on the phosphorylation of the Ser10 residue (47) . Recently, it was demonstrated that p27Kip1 phosphorylation induced by the oncogenically activated kinase Akt/PKB disables the nuclear localization capacity of p27Kip1 (33 , 34 , 41) . Akt/PKB can directly phosphorylate p27Kip1 at a Thr157 residue within the nuclear localization signal both in vitro and in vivo (33 , 41) . This Akt/PKB-dependent phosphorylation results in an impairing of its nuclear import, leading to cytoplasmic retention of the p27Kip1, abrogation of its CDK2 inhibitory activity, and cell cycle progression (41) . The Akt/PKB phosphorylation in primary human breast cancer statistically correlated with the cytoplasmic localization of the p27Kip1 protein (41) . An inhibition of the Akt/PKB activation with the phosphatidylinositol 3′-kinase inhibitor LY294002 or by the overexpression of DN Akt/PKB allele allows return of the p27Kip1 to the nucleus, resulting in an inhibition of CDK2 activity (33 , 34 , 41) . As with Ser10, Thr157-phosphorylated p27Kip1 is detected almost exclusively in the cytoplasmic compartment (34 , 41) . Whereas Ser10 phosphorylation promotes the nuclear export of the p27Kip1, Thr157 phosphorylation seems to impair its import into the nuclei (33) . The complex relationship between the p27Kip1 localization and degradation still remains to be addressed. Previous experimental evidence suggests that at least in breast cancer cells, the proteolysis and mislocalization of p27Kip1 occur through separate pathways (33) . Although the p27Kip1 phosphorylation at the Ser10 or Thr157 residue was not evaluated in this study, the close correlation between the cytoplasmic mislocalization of p27Kip1 and the constitutive Akt/PKB phosphorylation suggests that an Akt/PKB-mediated Thr157 phosphorylation mechanism is present in a substantial proportion of AML cases. In this study, the induced Akt/PKB activation resulted in a remarkable increase in the levels of cytoplasmic p27Kip1 in the U937 leukemia cells. Transfection of the U937 cells with a DN Akt/PKB construct resulted in a recovery of the nuclear localization of the p27Kip1 protein. These findings strongly suggest that Akt/PKB activation is one of the crucial mechanisms determining the localization of the p27Kip1 protein in leukemia cells. However, because the Akt/PKB phosphorylation was not observed in a certain proportion of AML cases showing the cytoplasmic mislocalization of p27Kip1 in this study, the contribution of other intracellular oncogenic pathways to the cytoplasmic mislocalization and dysregulation of p27Kip1 protein cannot be excluded.
Whatever the mechanism is, it is important to understand the biological significance of the cytoplasmic mislocalization of p27Kip1 in AML cells. The export of p27Kip1 from the nucleus to the cytoplasm serves to remove the inhibitory activity of p27Kip1 against cyclin E/CDK2 (34 , 41) . This allows an activation of an increasing number of cyclin E/CDK2 complexes, which are then free to phosphorylate the p27Kip1. The transfection studies suggested that the relocalization of the p27Kip1 from the nucleus to the cytoplasm was sufficient to sustain the cellular proliferation (34 , 41 , 48) . Another issue that needs to be addressed is whether the cytoplasmic mislocalization of the p27Kip1 observed in cancers merely contributes to abrogate its function or induces the p27Kip1 to acquire new cytoplasmic functions. The p27Kip1 was demonstrated to inhibit the drug-induced apoptosis (49) , although it is uncertain whether the nuclear and cytoplasmic p27Kip1 differ in their efficiencies to protect the cells from apoptosis.
Only recently, the cytoplasmic mislocalization of p27Kip1 in tumor cells has been identified as a mechanism whereby cancer cells promote carcinogenesis in humans (28) . Displacement of p27Kip1 into the cytoplasm contributes to the anchorage-independent growth of human transformed fibroblasts by maintaining high cyclin/CDK activity in the nucleus (31) . Cytoplasmic mislocalization of p27Kip1 has been reported for a number of human malignancies (26, 27, 28 ,, 33 , 34 , 41) . It appears that the preferential location of p27Kip1 in the cytoplasm of tumor cells is predictive of a more aggressive clinical behavior (33) . It was shown that the patients with Barrett's adenocarcinoma or breast carcinoma, which presented the cytoplasmic p27Kip1, showed decreased overall survivals compared with the patients who present with nuclear p27Kip1 (28 , 41) . In this study, the WBC count or cytogenetics, which had been generally considered as a prognostic factor in adult AML, was not significantly associated with prognosis. These findings were in agreement with the previous report, which revealed that neither WBC nor cytogenetics was an independent prognostic factor in adult AML (50) . Instead, we demonstrate for the first time that the p27Kip1 mislocalization is the strong prognostic factor in AML cases, whereas the total cellular levels of p27Kip1 do not have any prognostic value. The patients with high C:N ratio of the p27Kip1 localization showed a significantly shorter DFS and OS compared with the patients with low C:N ratio of p27Kip1 localization. The multivariate analysis revealed that the C:N ratio of the p27Kip1 localization remained an independent prognostic factor both the DFS and OS.
In conclusion, the cytoplasmic mislocalization of the p27Kip1 protein was observed in a substantial proportion of the AML cases. The mislocalization, not the cellular levels, of the p27Kip1 protein was highly associated with the constitutive phosphorylation of Akt/PKB and a poor prognosis in AML. Although the biological functions of cytoplasmic p27Kip1 protein and the genetic alterations that cause its mislocalization to the cytoplasm should be evaluated additionally, these findings suggest that the cytoplasmic mislocalization of p27Kip1 is an independent prognostic factor in AML, and the Akt/PKB-p27Kip1 pathway may be a ready target for antileukemia therapy.
We thank Dr. Y. G. Kwon (Yonsei University, Seoul, Korea) for providing the pcDNA3-myristoylated-Akt/PKB and pcDNA3-DN-Akt/PKB expression vectors.
Grant support: The 2001 Research Grant of the Institute of Genetic Science provided by Choongwae (Y. Min).
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Requests for reprints: Yoo Hong Min, Department of Internal Medicine, Yonsei University College of Medicine, Seodaemun-ku Shinchon-dong 134, Seoul 120-752, Korea. Phone: 82-2-361-5438; Fax: 82-2-393-6884; E-mail: minbrmmd{at}yumc.yonsei.ac.kr
- Received January 19, 2004.
- Revision received May 5, 2004.
- Accepted May 20, 2004.
- Published first August 2, 2004.
- ©2004 American Association for Cancer Research.


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Due to COVID-19 restrictions, our group classes are limited to 5 people per class per session.
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|1st Meeting Minutes
|2nd Meeting Minutes
|1st Meeting Minutes
|2nd Meeting Minutes
|1st Meeting Minutes
|2nd Meeting Minutes
|6th Newsletter
Lion Year 2016 - 2017 under King Lion Chris Stank
May 2017
Thanks to Lion John Behel and Lion Pete Sandys for the work completed on our club's road sign leading into Mechanicsburg! Once again, our sign is standing tall and proud communicating the club that serves!
May 16th Meeting
We welcomed our newest Lion Foss this evening!
Lion John and Lion Tim have a talk with our Lion Mascot Lion Foss
May 6, 2017
The Mechanicsburg Lions Club participated in the Mechanicsburg School District's "Literacy under the Lights" program on Friday, May 5th. Four Lions managed a "Free Vision Screening" service and "Color a LION" table for attendees. 30 children were screened at the 2 hour event with one child being referred for follow up eye care. Participating Lions were Susan Holder, Tawyna Kling, Chris and Cathy Stank.
May 5, 2017 (see write up and photos above)
The Mechanicsburg Lions Club will conduct a VISION SCREENING program at the Mechanicsburg School District's LITERACY NIGHT UNDER THE LIGHTS program on May 5th.
May 2nd Meeting Highlights
Guest speaker Lion Roy Davis, CEO & Chief Operating Officer at Lions Eyeglass Recycling Center of Northern Virginia made his presentation & answered many questions regarding eyeglass recycling. Thank you Lion Susan Holder for arranging this informative guest speaker.
King Lion Chris awards our thank you to guest speaker Roy Davis. Roy's wife is sitting on the right!
Demonstration pictures of the use of the New Digital Vision Screening Equipment
King Lion Stank on left ( observer ) Lion Diane Hartzell greeting Lion Tim Jr. ( Lion Tim Hooven ) and his father Lion Tim ( Lion Tim McCaulley) to the vision screening area ( Demonstration)
Lion Tim and Lion Tim Jr. await questions by Lion Jim who inputs the data into the Vision Screening equipment.
Lion Jim Miller adjusts vision screening equipment as father Tim looks on. In a few seconds the vision screening is completed and a print out of the results is available for the parent. Thank you Lion Tim Hooven for assisting in this demonstration, and also Lion Tim McCaulley and all others involved!
April 22, 2017
Check King Lion on TV Fox 43: http://fox43.com/2017/04/23/mechanicsburg-earth-day-festival-offers-interactive-learning-for-families/?share=email&nb=1
Several members of the Mechanicsburg Lions Club participated in 2 Earth Day events this week. In the focus category of ENVIRONMENT, the programs included a tree seedling wrapping event and Earthday downtown Mechanicsburg. The seedling wrapping was conducted at Shillelaghs farm in Mechanicsburg. The Lions assisted in the wrapping of 6000 white pine seedlings to be distributed to local schools as well as handed out at the Earth Day event in downtown Mechanicsburg. The Earth Day activity took place on Saturday and was the 2nd year that our club participated. We managed a booth where kids and adults were encouraged to write Earth Day focused messages and pictures on paper grocery bags from Giant Food stores. The bags were collected and will go back to Giant stores and will be used for customers groceries. They get a bag with an Earth Day message and artwork! The Lions who participated in these programs, donating over 30 service hours ,were: Tim McCaully, Leroy Weaver, Susan Holden, Bob Smith, Tawyna Kling, Cathy Stank and Chris Stank.
Photos from Tree Seedling Wrapping
Photos from Earth Day 2017
Members of the Mechanicsburg Lions Club participated in the annual Spring Clean up Day at the Lion's Club's Beacon Lodge, a camp for blind and disabled children and Veteran. The Mechanicsburg Lions who joined volunteers from several other Lions Clubs were: Leroy Weaver, Charlie Rixey, Pete Sandys, and Chris Stank. The project that the 4 Lions worked on was to repair a creek bridge that was damaged by a fallen tree. The project was completed on Saturday, April 8. Please visit the Beacon Lodge Website to learn more about the facility!
The Mechanicsburg Lions club has conducted a Kidsight Vision Screening program at the Mechanicsburg Kindergarten Academy registration. Over the 3 day registration this week, the Lions, using state of the art equipment, performed vision scanning on 238 children. Of those scanned, 30 children received referrals for follow up eye exams.
The Lions who worked the 3 day scanning program were: Terry Hartzell, Dianne Hartzell, Jim Miller, Sue Miller, Susan Holder, CeCe Viti, Cathy Stank and Chris Stank. Assisting the Team were Lions Gloria and George Hilsinger
April 4, 2017 Open house featuring guest speaker Barbara Landis from the Cumberland County Historical Society, giving her presentation on the Carlisle Industrial Indian School.
Lions and guests at dinner
PDG Fred Lamason, wife Sue and guest speaker Barbara Landis from the Cumberland County Historical Society getting ready to present her talk on the Carlisle Industrial Indian School.
Lion Secretary Ted presenting Lion Dr. Erica Voss-Meloy with her 20 year Monarch certificate and pin.
Posters of Lionism
King Lion Chris presenting Lions' gift to Barbara Landis of the Cumberland County Historical Society
March 20, 2017
In celebration of the Lions Clubs 100th anniversary, the Mechanicsburg Lions Club partnered with residents of Traditions of America, Silver Spring, on a special project to help Pink Hands of Hope. The project, led by Lion Cathy Stank, made 100 "Port and Comfort" pillows for Women in the fight. The pillows were presented to Brian Gaughen at the Pink Hands of Hope Location on Monday, March 20.Lions Cathy Stank, Lion Sue Miller, Lion Susan Holder and Lion Lana McCaulley. TOA volunteers included Nancy Sandys, Cindy Cramer, Jeanne Dippel, Elaine Hellen
Club members who worked on the project making the pillows were:
Our March 14th meeting is CANCELLED due to snow storm!
Give From the Heart program held today at Upper Allen Elementary School.
A first this year was the attendance of District Governor Kerry McKnight & a
Another new for this year was the donation of special pins to commemorate the event -
Many thanks for Lions Tim & Lana McCaulley for designing the pins & donating them
so every student got a very nice keepsake!
Outstanding effort by our Lions helping our youth realize that helping others is an important aspect of our lives!
Great work Lions!
2-18-2017 King Lion Chris taking glasses collected for recycling .
Our additional helpers at the Bon-Ton! Seeing Eye Puppies in training! February 17, 2017
2-11-2017 Lion Duke Brenneman on left and Lion Tom Kerstetter selling Community Days Coupon books at the Bon-Ton.
Our condolences to PDG Noah E Krout's family on his passing from our Lions Club family of Mechanicsubrg.
Special Message from Lion Secretary Ted:
ATTENTION Lions: March is Diabetes Awareness Month, and we have a Diabetes Awareness Speaker for you at our March 14th make-up meeting. Barb Ruth, a Pinnacle Health Diabetes Educator will be visiting us on March 14th with important information about Diabetes. Many of us are effected and many of us don't know. Please plan to attend.
King Lion Chris Stank presenting a check to Jeanne Troy from New Hope Ministries. at our first meeting 1/03/17 of the new year!
Christmas Party Dec. 20th, 2016
Winners of the "Ugly Christmas Sweater" Contest
(From left: Lions Terry Hartzell ( Ugliest) & wife Diane Hartzell (Rash Inducing), Mary Lou Kerstetter (Most Flamable) and Linda Fenicle (most creative). Congratulations to all contestants.
A Little business below:
PDG Fred Lamason Installs our Three New Members
From the left: Lion Sam Robertson, sponsor of new Lion Tim Hooven. Lion Jim Miller, sponsor of new Lion, his wife Sue Miller. And, Lion PCC Terry Hartzell, sponsor of new Lion Diane Hartzell, his wife.
And presents were enjoyed by all !
Our new member Tim, and his wife to be, Stephanie!
Our Lions and guests at their Tables Getting Presents from our Chinese Auction
Click here for our Facebook Christmas Party photos
December 15, 2016
For the second year, the Mechanicsburg Lions Club has sponsored a program with Fifth Grade classes of Upper Allen Elementary school . The program, "Give from the Heart" , introduces the kids to the concept of being charitable and being helpful to others. Each of the 4 Fifth grade classes are given the opportunity to donate $100 to a cause that they choose. The teachers manage the program and the kids team up and do research to determine the recipient of the donation. They make presentations to their classmates and vote on the recipient. The choosing of recipients takes place from November to December. The recipients have just been announced and they are: Pink Hands of Hope, ASPCA, Hope for Paws, and Charity-Waters. An assembly will be held in February and the kids will present the checks to a representative of the charity they chose. Each of the 5th graders will receive a special commemorative pin that was designed by Lion Tim McCaulley. The pin, depicting a fun Lion with a Lions Logo and the Mechanicsburg Lions Club on a heart will serve to remind the kids of this experience and further communicate the message of being charitable.
December 15, 2016
The Mechancisburg Lions Club answered the call for assistance from the "Angel Tree Project" for the Cumberland Valley School District by providing vehicles and Club volunteers to deliver donated Christmas gifts to 4 schools. The donated gifts will go to over 200 needy children for Christmas. The volunteers from the Mechanicsburg Lions Club included Lion Ted Cramer, Lion Sam Robertson, Lions Tim and Lana McCaulley, and Lions Chris and Cathy Stank.
December 1, 2016
Mechanicsburg Lions Club volunteers (King Lion Chris, Lions CeCe, John, and Jim ) at the Wildcat Foundation community Day held Saturday, Nov 19th.
Lion Weaver Reminds Us:
We will collect items for New Hope Ministries again this holiday season. Members should bring items to either meeting in December. Any non-food items like toilet paper, paper towels, etc. plus food items such as P-Nut Butter, Macaroni & Cheese and Hamburger Helper.
We have had a very busy schedule this fall:
The Downtown Mechanicsburg Fall Clean Up was held on Sunday November 13th at 2 PM. Volunteers from our club were Lion CeCe and Lion Susan and husband Dallas! Thank you for your participation!
November 5th at "Touch of the Valley"
King Lion Chris setting up at "Touch of the Valley" for the Eagle Foundation
"Touch of the Valley"
From Left: Lions Sam, Tim, Lana, Cathy, and President Chris
At our Nov 1st meeting we had as guest speaker PDG and current 14C LCIF Chair Noah Krout and his wife Becky Krout .
PDG and Current LCIF Chairperson Noah Krout on your left with our King Lion Chris Stank.
Becky Krout and PDG and Current LCIF Chairperson Noah Krout with our King Lion Chris Stank.
Thanks to the funds the Mechanicsburg Lion's Club contributed, I finished the bulk of my Eagle Scout Project today. Over the past two weekends, I held work days to complete the concrete pad for the grill and the split rail fence. I will send you an invitation to my Court of Honor in the spring as soon as it is planned. So you can see some of the work, I have attached a few pictures of the work days. Again, thank you so much for your generous donation.
Cam Morgan
BSA Troop 51
Life Scout
The Mechanicsburg Lions Club is selling BonTon shopping passes at the Camp Hill BonTon store. November 9,10,11,&12!
People can also purchase these passes on BonTon's website and direct the purchase to the Lions Club.
Mechanicsburg Lions President Chris Stank
Friday Night, October 14th at CV vs Carlisle Football Game
Lion Sam and Seeing Eye Dog with trainers at CV vs Carlisle Football Game
Boscov's Fund Raising 2016
Lion CeCe at our Boscov's Fund Raiser Oct 15th 2016
On Saturday, October 15th the Mechanicsburg Lions Club will meet in front of Ritter's Hardware Store on Main Street from 8AM - 4 PM to collect contributions for the blind and to participate in "Streets of Treats Day" in Mechanicsburg. We look forward to as much Lion participation as possible. Come and enjoy the fun of spreading the meaning Lionism.
Here are the Lions having fun at White Cane Day Oct 15th
Lions Ted and John
Lions Tim, Lana, John & Tom enjoying the cool morning
Ho Ho says the jolly old pirate at White Cane Day & Streets of Treats Day
Lion Tim with Halloween Oriole on White Cane Day
Super Girl Visits our stand on White Cane Day
Princess rides the wagon on White Cane Day and Streets for Treats Day in Mechanicburg
From the left, Lion Deb McKnight, our Lion Treasurer Lana, our Zone Chairman Mike, DG Kerry Mcknight, King Lion Chris, and Lion Treasurer Ted.
King Lion Chris on right presenting our check to DG Kerry.
DG Kerry McKnight presenting Past President John Behel with membership key award for sponsoring 2 members.
14-C Membership Growth Meeting at the Jacobus Lions Club.
August Meeting - Special Location! - Club Field trip
Our Club had a special off site location for the August 9 meeting!
The Mechanicsburg Lions Club conducted a "Field Trip" to The Vision Resources headquarters, in Harrisburg, where we conducted our August Summer meeting. After a catered dinner, we received a presentation and tour by Danette Blank , Executive Director, and Cheryl Cuddy, Director of Community Relations. A productive meeting and a very educational tour!
"The Mission of Vision Resources of Central Pennsylvania is to support and promote the interests of people who are blind, visually impaired and otherwise disabled, and to provide blindness prevention services."
On Saturday, July 23rd, 8 members from the Mechanicsburg Lions Club volunteered to assist at the Tri4Kids at Memorial Park in Mechanicsburg. The volunteers assisted the Mechanicsburg Recreation department at this youth focus program. Pictures include the following Lions:
Cece Viti
Steve & Danielle Harriman
Terry Hartzell
Tim McCaulley
Bob Smith
Chris & Cathy Stank
Our Lions in Action
Pink Hands of Hope needed quick response for assistance due to being short handed because an employee had a serious family emergency. Lions Susan, Cathy , and King Lion Chris pitched in to help the non profit get caught up on inventory sorting.
Lions Cathy Stank on left. King Lion Chris Stank center, and Lion Susan Holder on right.
Installation Night 2016 pictures:
New King Lion Chris Stank receiving gavel from King Lion John Behel
Lion Secretary Ted presenting the Melvin Jones Fellowship Award 2016
to Lion Mark Basehore
Lion President John Behel left, and Lion Secretary Ted Cramer sponsor of new Lion Darla Romberger and New King Lion Chris Stank sponsor of new Lion Cathy Stank.
(Not present: Pete Sandys sponsored by King Lion Chris; and Sam Robertson sponsored by Lion Tom Kerstetter)
Mechanicsburg Little League honors our club for 64 years of sponsorship.
Mechanicsburg Little Leage coach, George Spangler and two of his players present the thank you plaque for 64 years of Mechanicsburg Lions Club sponsorship!
Please click below to view more photos on our Facebook page.
** thank you Jeannette Behel and Mary Lou Kerstetter for most of the installation photos!


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Cops: Known gang members steal deputy's car, uniforms, rifles
Barbara Hijek (Sun Sentinel) | January 6, 2011
Palm Beach County Sheriff's Deputy Luis Colon was on vacation Sunday night when two teens, who police say are known gang members, burglarized his home in Port St. Lucie, reports TCPalm.com.
Police on Sunday went to Colon's home. The garage door was open, as well as a door leading from the garage into the home. Another rear glass door was smashed, reports TCPalm.com.
A 2006 Toyota Sequoia, which is equipped with the LoJack tracking system, was missing. So were two rifles and some law enforcement uniforms, according to TCPalm.com.
A St. Lucie County sheriff's deputy tracked the Toyota's LoJack signal to an abandoned home used to store the stolen uniforms, a surround sound system, ammunition and other items, reports TCPalm.com.
More at: http://weblogs.sun-sentinel.com/news/specials/weirdflorida/blog/2011/01/cops_known_gang_members_steal.html
Image: Peter Henry Harris / St. Lucie County Sheriff's Office


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Swedish House Mafia will play Tomorrowland 2019 "by any means necessary", according to Steve Angello
Swedish House Mafia will play Tomorrowland 2019 "by any means necessary", according to Steve Angello.
The EDM star – and one third of the chart-topping trio – revealed the news in a new interview with Dutch newspaper, De Telegraaf.
Tomorrowland's Sunday weekend two streams are now live: watch
Tomorrowland's annual stream has now gone live for the final day of its weekend two celebrations, with the mega festival set to stream music from Martin Garrix, Steve Angello, Nina Kraviz, Richie Hawtin, Solomun and many more.
The festival, which is based in Boom, Belgium, has been broadcasting again across four channels for three consecutive days this weekend (27th to 29th July), after doing so at last weekend's event (22nd to 24th July).
Tomorrowland's Sunday streams are now live: watch
Tomorrowland's annual stream has now gone live for its weekend one celebrations, with the mega festival set to stream music from Steve Angello, Waff, Kolsch, Solardo, Camelphat and many more.
The festival, which is based in Boom, Belgium, is set to broadcast across four channels for three consecutive days this weekend (20th to 22nd July), as well as next weekend (27th to 29th July).
Today's highlights include sets from Timmy Trumpet and Steve Angello on the Main Stage, plus Jax Jones and Martin Solveig on the My House stage.
Steve Angello: 'Touring is tough, taxing and unhealthy for artists'
Steve Angello has spoken about the pressures of touring and the effects it can have on even the most successful artist's mental health.
In a recent interview with Swedish publication Aftonbladet's digital radio segment, the Swedish House Mafia member was asked about how the recent death of his friend and contemporary Avicii aged just 28 had affected him.
Swedish House Mafia return teased with hint on advertising billboard
Axwell Λ Ingrosso dropped a new track, titled 'Dancing Alone', last week (29th June).
The duo have now used a billboard advertising the release to seemingly tease the return of Swedish House Mafia – the trio made up of Axwell, Sebastian Ingrosso and Steve Angello.
The billboard, which you can see below, first shows the 'Dancing Alone' artwork, followed by an error message along with the word "loading", before Swedish House Mafia's trademark white dots are shown.
Steve Angello & Axwell Λ Ingrosso announce new music out Friday
Steve Angello and Axwell Λ Ingrosso all reportedly have new music landing this Friday 29th June.
Kryder revealed that his Angello collaboration, titled 'Romani', lands via his new label Kryteria Records on Twitter last night (26th June).
EDM Sauce also reports that Axwell Λ Ingrosso's long-awaited 'Dancing Alone' will also land on Friday. This is yet to be confirmed officially.
Martin Garrix and Steve Angello are playing a huge stadium show together this summer
Martin Garrix and Swedish House Mafia's Steve Angello are teaming up for a late-summer blowout at Belfast Vital music festival.
The 25th August show will take place at Boucher Road Playing Fields and features Dutch EDM star Garrix as headliner. But some very special guests will also join him on the last Saturday of the season.
Swedish House Mafia's Steve Angello, Swedish pop star Zara Larsson and fast-rising talent Loop have all been confirmed as support for the night.
Swedish House Mafia 2019 tour confirmed by Steve Angello
Steve Angello has confirmed that Swedish House Mafia will embark on a tour next year.
Swedish House Mafia tease 2019 tour dates
Swedish House Mafia look set to be going on tour next year after Axwell ^ Ingrosso teased fans at a show in New York City's Brooklyn Mirage on Memorial Day (28th May)
Toward the end of the set the pair, who form two thirds of Swedish House Mafia, stopped the music and announced that there are indeed gigs lined up for the trio next week.
Ultra Singapore locks Above & Beyond, DJ Snake, Loco Dice, more
Ultra Singapore has locked trance giants Above & Beyond, big room big guns like DJ Snake, Afrojack and Steve Angello, and techno heads such as Loco Dice and Nastia for the two-day festival, which runs on 15th and 16th June.
The event, which is held in the fittingly-named Ultra Park, also boasts Fedde Le Grand, Nervo, Illenium, Nicky Romero and Coyu. 2018 marks its third edition, and will see the talent on offer spread across four main rigs— Mainstage, Resistance, Worldwide and Ultra Angels.


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This site uses cookies. Sexually explicit or offensive language. This thread is locked. This site in other languages x. I could never get the 3C to work with Vista, let alone Win 7.
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Forums New posts Search forums. That hardware compatibility website is based on user-reported results, which are prone to error. I tried this and made Vista search drivers again from the DVD, but again it did not find anything.
You can follow the question or vote as helpful, but you cannot reply to this thread.
3C905 driver for 64 bit win 7 or Vista
Click on the following links for the driver package readme info: You can help protect yourself from scammers by verifying that the contact is a Microsoft Agent or Microsoft Employee and that the phone number is an official Microsoft global customer service number. Thanks for marking this as the answer. After that you can go and check the driver version and it looks like this: Promote cracked software, or other illegal content.
I've seen several peripherals that were erroneously reported as 'compatible' as well as 'incompatible' devices that work just fine. Home Windows Drivers Network Drivers 3cc. Windods reported, our staff will be notified and the comment will be reviewed.
Solution: 3COM 3CTX drivers for Vista | Vista Forums
Click here to review our site terms of use. Just used this same solution on the Windows 7 Beta.
How satisfied are you with this response? So this was only for 3C adapter, now there is only I again found nothing Vista related from there. Malke Jan 13, Login or 3c905-fx an account to post a review. I soon found serveral others looking 3COM Vista drivers also.
This site uses cookies. I could never get the 3C to work with Vista, let alone Win 7.
I'm from Brazil and was looking for a solution, with the driver for Vista. The 3C is at least 12 years old, BTW. Alysson May 19, The driver will be generic at best and will not enable best performance assuming ttat you get it to work.
Solution: 3COM 3C905-TX drivers for Vista
Sorry this didn't help. I have the same 3cim Advertisements or commercial links. But once you windlws got to the web you can make Vista to look even newer driver, it even seems to find something and installs this kind of driver "File version: How satisfied are you with this reply? But its so difficult to find these drivers yet.
I'll try your tip Since you've already submitted a review for this product, this submission will be added as an update to your original review.
Time to start Googling to get this sluggishly slow Vista up and networked.