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Review ARTICLE
Transcriptomic and Proteomic Tools in the Study of Hg Toxicity: What Is Missing?
- 1Programa Pós-Graduação Stricto Sensu em Biotecnologia Aplicada a Saúde da Criança e do Adolescente, Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Brazil
- 2Faculdades Pequeno Príncipe, Curitiba, Brazil
- 3Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, Brazil
- 4Department of Molecular Pharmacology, Albert Einstein College of Medicine, New York, NY, United States
Mercury is a hazardous substance that has unique neurodevelopmental toxic effects in humans. However, the precise sequence of molecular events that culminate in Hg-induced neuropathology is still unknown. Though the omics studies have been generating an enormous amount of new data about Hg toxicity, our ability to interpret such a large quantity of information is still limited. In this opinion article, we will reinforce the necessity of new high throughput and accurate analytical proteomic methodologies, especially, thiol and selenol-proteome. Overall, we posit that improvements in thiol- and selenol-proteomic analyses will be pivotal in identifying the primary cellular targets of Hg. However, a better understanding of the complex cascades and molecular pathways involved in its toxicity will require extensive complementary studies in more complex systems.
It is well established that the chemical forms of Hg (Hg0, Hg2+, MeHg+, EtHg+) differ in their toxicokinetics and toxicodynamics (Syversen and Kaur, 2012). Moreover, the interaction of electrophilic forms of Hg with –SH or –SeH groups of the biomolecules can cause: (1) accelerated rate of inactivation of specific –SH– or –SeH–containing molecules (Oliveira et al., 2017, 2018; Kuras et al., 2018; Branco and Carvalho, 2019); (2) a decrease in the availability of Se for selenoprotein synthesis (Barbosa et al., 2017; Bjørklund et al., 2017; Oliveira et al., 2017, 2018; Ralston and Raymond, 2018; Nogara et al., 2019a,b); and (3) increased rate of Hg entry into cells (for example, MeHg-cysteine and cysteine-Hg-cysteine as a mimic of methionine and cystine, respectively) (Bridges and Zalups, 2010, 2017). Nonetheless, to better characterize the toxic effects of Hg, it is essential to understand its primary toxic target(s), which, in turn, will trigger a cascade of events culminating in cellular demise and pathophysiological changes. In this context, the advent of the omics approaches can be instrumental in unveiling primary toxic target(s) of Hg, and to identify the pathways activated in response to the interaction of Hg with these targets.
Based on the cationic Hg and –SH/–SeH chemical properties, it is well accepted that the primary Hg targets are the –SH/–SeH-containing proteins; however, our knowledge about which of them are the primary toxic target(s) and the precise molecular pathways involved in the toxicity of Hg remain elusive. In our opinion, both –SH and –SeH transcriptomic and proteomic analyses have to be taken into consideration in search of Hg's primary toxic target(s). To our knowledge, the –SH–transcriptomic approaches cannot identify yet which of the cysteine codons (UGC and UGU; Rocha et al., 2017) will be free -SH in the nascent and functional proteins. However, the presence of a codon for cysteine in the mRNA point to a potential site for cationic Hg species binding. Indeed, any transitory free –SH group in a nascent protein (cysteinyl residues that will form a disulfide bond in functional proteins) are potential targets for cationic Hg species. On the other hand, the –SeH-transcriptomic analyses can provide a more precise view of potential targets for Hg-induced selenoprotein disruption, once practically all the selenocysteine residues will be in the –SeH form (Hatfield et al., 2014), i.e., free to react with cationic Hg species.
Despite the instrumental role that omics approaches may have in deciphering the targets and pathways involved in the toxicity of Hg, only a limited number of studies have evaluated the effect of Hg on the –SH– or –SeH-transcriptome and/or proteome and fewer made use of in silico analysis or artificial intelligence (AI) approaches. Until now, the transcriptomic data have provided valuable information on altered gene expression and/or regulation, but have failed to delineate whether these alterations are the cause or effect of Hg toxicity. Moreover, detailed studies on the time course of Hg-induced changes in gene expression, which are essential to figure out the first toxic target(s), have been rarely performed (Robinson et al., 2010; Theunissen et al., 2011). Therefore, the availability of the raw data in public databases is not mandatory for transcriptomic studies and several researchers have not deposited their data publicly, making this another limiting factor in the study of Hg toxicity. In addition, the detection of differential expression of specific sets of RNAs after exposure to very low to low levels of Hg (10–21 to 10–13 M) is highly needed to identify possible "expression signatures" that might be used as early markers of Hg exposure, much earlier than physiological or biochemical changes can be detected. Here, particularly for the very low levels of exposure, we will face additional analytical constraints, for instance, the necessity of new ultrasensitive methodologies capable of detecting trace quantities of R-Hg-proteins adducts. These limitations conceivably will require complementary omics- and non-omics approaches to be achieved.
mRNA and protein expression levels cannot be expected to correlate perfectly due to post-transcriptional regulation. Consequently, proteomic analyses can provide a more realistic view of the protein content in a determined developmental stage or pathophysiological condition (Fu et al., 2009), for instance, in the cases of Hg intoxication. In this context, the development of new proteomic techniques able to detect the primary toxic target(s) of cationic Hg species is pivotal.
The human selenoproteome and cysteine proteome were already determined (Jones and Go, 2011; Labunskyy et al., 2014; Go et al., 2015). Nonetheless, the development of reliable high throughput thiol-omics and selenol-omics analytical methodologies are highly needed to determine which of the proteins containing reactive and biologically relevant –SH and –SeH will interact with cationic Hg species. This will be instrumental in distinguishing proteins that are effectively involved in the genesis of Hg pathology from those that bind Hg but did not change the cell, tissue, and/or organism biological functions.
In the proteomic context, analytical methods have been developed to identify the redox-sensing thiols. The standard redox-sensitizing thiol workflow is based on (1) the treatment of the proteins with electrophilic compounds and, then, with thiol markers (labeling step), (2) a 2D gel electrophoresis is carried out, and (3) the selected proteins are separated from the gel and digested (proteolysis) to further LC-MS/MS identification. Cationic Hg species bound to proteins can be quantified by gel electrophoresis through the fluorescent labeling assay (for example, the BPM/biotin-PEAC5-maleimide labeling assay), where a decrease in the fluorescence reflects a decrease in the number of free –SH groups, i.e., an increase in the number of Hg-protein adducts (protein-S–Hg-R) (Toyama et al., 2013). In addition, with LC-MS/MS analysis, it is possible to identify cysteine residues to which Hg species are bound. However, the steps vary, depending on the methodology selected (Leichert et al., 2008; Hill et al., 2009; García-Santamarina et al., 2011; Jones and Go, 2011; Karlsen et al., 2014; Zaccarin et al., 2014). Nevertheless, concerning the –SeH proteomics, there are still no defined techniques. In the case of –SeH groups, the methodologies will have to take into account the very low level of occurrence and the extreme reactivity of the –SeH/–Se– groups. The instability of the –C–Se– bond (Orian et al., 2015) in the Sec–Hg–R adducts is also of great analytical concern.
A persistent technical problem that compromises the identification of specific –SH groups targeted by Hg species is the necessity of denaturing the protein with detergents and reducing agents before the proteolysis, for instance, DTT or tris(2-carboxyethyl)phosphine (TCEP). This step is performed to unfold the protein and improve the fragmentation; however, the addition of high concentrations of –SH reducing agents can remove the cationic Hg species from proteins via an exchange reaction (protein-S-Hg-R + R-SH → protein-SH + R-S-Hg-R; Nogara et al., 2019b).
Of particular importance, the use of omics in toxicology can be limited by methodological uncertainties in interpreting and assessing data (Buesen et al., 2017). The use of transcriptomic and/or proteomic analyses is recent, and only a few studies have attempted to outline questions relevant to apical end-points of Hg toxicity. The majority of the studies that link differences in gene expression or protein activity/level with Hg toxicity have not used omics technologies. Indeed, omics technologies have been only employed in an exploratory way. The systematic application of omics analysis to simple relevant in vitro studies, followed by invertebrate models, will be crucial in generating more comprehensive and workable data capable of driving the elaboration of appropriate omics studies with vertebrates. A similar type of study to be followed as an example was carried out with manganese (Fernandes et al., 2019).
It will be crucial to explore different doses/concentrations of Hg, e.g., from very low (10–21 M) to high (10–6 M). As commented above, the analytical determination (R-Hg-protein adducts and/or total Hg) at very low Hg levels will be a challenge to be reached by the researchers; however, the cellular response can ideally be followed by cutting-edge cellular and molecular methodologies. Exposing cells to very low concentrations/doses of Hg for a short time will afford the opportunity to detect a possible compensatory hormetic mechanism, which can be interpreted as molecular responses resulting in cellular protection. With increased doses (concentrations) or exposure time, the compensatory mechanism can be exhausted, and the ensuing damage will manifest (Calabrese and Baldwin, 2001). These efforts will be pivotal in the understanding of the first signs of Hg toxicity and, consequently, will be helpful in the identification of the first target of cationic Hg species. A virtual protocol of exposure is depicted in Figure 1, using as an example methylmercury (MeHg+) the Hg organic chemical form ubiquitously present in fish.
Figure 1. Hypothetical toxicogenomic studies using simple in vitro models. Here relevant human cell types have to be exposed from very low (10–21 M) to high (10–6 M) concentrations of MeHg+. For instance, non-differentiated or differentiated neurons or glial cells have to be incubated with MeHg+ and physiologically relevant MeHg-S conjugates (cysteine, GSH, albumin or hemoglobin, as an example see Tan et al., 2019) for different periods. MeHg+ toxicity will have to be determined by proteomics, specifically by the –SH– and –SeH-omics, epigenomics, metabolomics, and transcriptomics. In short, even for a single type of cell, the number of systematic analyses to be performed is arduous. They will allow the construction of a causal relationship between gene regulation/expression with protein and metabolites levels. Using these data (nucleotide and amino acid sequences) and AI algorithms, particularly machine learning, could be possible to identify the tertiary protein structure (e.g., thiol- and selenoproteins) and predict the motifs that will be more likely disrupted by MeHg+ facilitating the understanding of potential proteins involved in MeHg+ toxicity and consequently the first toxic target(s).
To better understand the enormous amount of data that will be generated from the time course and dose/concentration variation studies, the in silico analyses will be necessary. These types of analyses have been sparsely explored in Hg studies. The analyses have to include interaction modeling and pattern searching using AI algorithms, particularly machine learning and deep learning. In the future, machine learning should be used to identify/predict the tertiary protein structure from transcriptome (nucleotide sequence) and proteome (amino acid sequence) studies. Considering the scarcity of detailed tertiary/quaternary protein structures availability, in silico prediction models, will be crucial to identifying the preferential Hg binding sites, i.e., to construct models that can reveal the electrostatic environment of solvent exposed –SH and –SeH moieties. The identification of accessible and preferential Hg binding sites in tertiary predicted structures will allow identify electronic and chemical motifs that will be more likely disrupted by Hg, and which amino acids are more likely around these regions. The knowledge of such sequences will facilitate the identification of potential proteins involved in Hg toxicity. Upon identifying potential Hg target proteins using specific proteomic techniques (e.g., selenoproteins and thiol-containing proteins that have CC, CXC, and CXXC motifs), the use of knockout models in a systematic way will be useful for directly analyzing the role of specific thiol- and selenoproteins in the toxicity of Hg.
Thus, it is possible to conclude that to understand better the Hg cascade of events that will generate symptoms of toxicity, for example, irreversible symptoms, as observed in the Minamata disease, the researchers from different areas (Toxicology, Molecular Biology, Genetics, Biochemistry, and Bioinformatics) will have to join efforts. Punctual studies have been instrumental in understanding specific end-points of toxicity caused by Hg; however, only multidisciplinary studies will be able to figure out the first toxic target(s) of cationic Hg species. In addition, it will also be necessary to advance the knowledge of pathways and activation cascades in a physiological situation to understand the deregulation caused by xenobiotics, especially Hg, in more complex systems.
Author Contributions
CO, AS, BP, and JR were responsible by the manuscript main idea. PN was responsible by the figure. ÉL, MA, and JR were responsible for the English grammar corrections. All the authors actively participated in manuscript writing.
MA was supported by grants from the National Institute of Environmental Health Sciences, NIEHS R01ES07331, NIEHS R01ES10563, and NIEHS R01ES020852. The authors would like to thank the financial support of the Brazilians' development agencies: CNPq, FAPERGS/DOCFIX 04/2018, CAPES/PROEX (23038.005848/2018-31; 0737/2018), CAPES/PRINT, and INCT-EN: For Cerebral Diseases, Excitotoxicity, and Neuroprotection.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Keywords: methylmercury, proteome, transcriptome, neurotoxicity, thiol, selenol
Citation: Oliveira CS, Segatto ALA, Nogara PA, Piccoli BC, Loreto ÉLS, Aschner M and Rocha JBT (2020) Transcriptomic and Proteomic Tools in the Study of Hg Toxicity: What Is Missing? Front. Genet. 11:425. doi: 10.3389/fgene.2020.00425
Received: 19 August 2019; Accepted: 06 April 2020;
Published: 05 May 2020.
Edited by:Sandra Ceccatelli, Karolinska Institutet (KI), Sweden
Reviewed by:Fusako Usuki, Kagoshima University, Japan
Matthew D. Rand, University of Rochester, United States
Joëlle Rüegg, Uppsala University, Sweden
Copyright © 2020 Oliveira, Segatto, Nogara, Piccoli, Loreto, Aschner and Rocha. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: João B. T. Rocha, firstname.lastname@example.com


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I'm creating a site for events where people can subscribe themselves to a single one. Now, I'm using Node Registration module to allow users to subscribe. I would like to know if it is possible to enable comments of a particular event only to users who are members of it. Is there a module to do this? I tried with rules module but i couldn't...
do anonymous users allowed to register to events? – arpitr Nov 18 '13 at 12:12
No, only registered users can subscribe to an event...if you are already a site member you can add comments only to events where you are subscribed in.. – Luke Nov 18 '13 at 14:25
check the answer added below if that helps you – arpitr Nov 18 '13 at 14:27
Here is a programmatic way to do this, add this code to your custom module
In the following code my content type name is article, change it to yours.
Also this code can not handle functionality for anonymous user as I couldn find a way to track if anonymous user is already registered or not.
* Helper function to check if user is registered to a given event node or node.
function check_if_user_is_registered($node, $uid) {
$return = FALSE;
$registrations = node_registration_load_multiple(FALSE, array(
'uid' => $uid,
'nid' => $node->nid,
'cancelled' => 0,
if(is_array($registrations)) {
foreach($registrations as $registration) {
if($registration->author_uid == $uid) {
$return = TRUE;
return $return;
* Implements hook_node_load().
function MYMODULENAME_node_load($nodes, $types) {
global $user;
foreach ($nodes as $node) {
if($node->type == "article") {
$access = check_if_user_is_registered($node,$user->uid);
if(!$access) {
$node->comment = 0; // to close comments
Thanks! but I have only used existing modules (node registration modules), not custom ones...How can i do? Do you suggest me to create a new one by myself? – Luke Nov 18 '13 at 14:32
check this packtpub.com/article/… – arpitr Nov 18 '13 at 14:34
basically you need two files mymodulename.info which tells drupal about your custom module and mymodulename.module where your funcitonality lays down. remember "mymodulename" should be replaced by a standard name mostly the name should revolve around the functionality it adds.So the above code in the answer will go to the your .module file and the link shared in the comments can tell you what to add in the .info file – arpitr Nov 18 '13 at 14:38
Thanks for the answer! Now i'm going to read it. I hope to understand.. – Luke Nov 18 '13 at 14:39
sure let me know if you have issues – arpitr Nov 18 '13 at 14:45


text:
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Chubb is first global property and casualty insurer to invest in Africa's leading multilateral political risk and credit insurance agency NEW YORK and NAIROBI, Kenya, Dec. 10, 2019 /PRNewswire/ — Chubb and the African Trade Insurance Agency (ATI) today announced that Chubb
Elkay Interior Systems acquires European-based seating and décor businesses Schloffer GmbH, Designed2Work, and Designed2Work Intl.
Move expands the company's growing international branded retail design and builds capabilities OAK BROOK, Illinois, Dec. 10, 2019 /PRNewswire/ — Representatives of Milwaukee, WI-based Elkay Interior Systems (EIS) today announced the American company's acquisition of Schloffer GmbH, a global seating and
Dickey's Barbecue Pit Headed to Africa
CAIRO, Dec. 9, 2019 /PRNewswire/ — Dickey's Barbecue Pit is bringing authentic, Texas-style barbecue to Egypt with a planned opening in Cairo in late spring/early summer next year. The world's largest barbecue franchise signed with the Swinder Group to introduce
Foreign Minister and GCC Secretary General Hold Press Conference 2 Riyadh
For his part, the Secretary General of the Gulf Cooperation Council Dr. Abdullatif bin Rashid Al-Zayani, affirmed the strength, cohesion and strength of the GCC, and the unity of the class among its members due to the special relations and


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Thanks Decky. I'll keep this in mind as I troubleshoot.
@andyr, interesting. I really appreciate the offer. I have a friend nearby who has an isolating transformer. I'll see if i can borrow it and see how that goes.
I'm rather new to separates and I'm sure this is embarrassingly simple. When plugging headphones into my receiver (Marantz AV7005), there's no sound coming through. Not sure if this is related, but it's contrary to what I would expect. When I'm playing music via the receiver, on plugging in the headphones (with jack adapter) the sound doesn't cease playing through the speakers. It's leading me to suspect the headphone port is just not picking up any connection. Am I missing something obvious here?
Thanks. I've ordered one online. I'll see how this goes.
TV antenna cable causing hum
Pennines posted a topic in General AV DiscussionI was fiddling with my cables the other day and suddenly I've been punished with a high pitched buzz/hum. After unplugging everything and slowly putting things back together, it doesn't appear the noise is resolving itself. I've searched a number of older posts and I haven't been able to find a solution to the particular situation I've found myself. I've simplified my setup down to the TV, 2 speakers, amp + receiver and have identified the culprit is originating from the TV antenna cable. Everything is also plugged into the one outlet (via powerboard). The cable is a standard RF antenna cable I've purchased from JB and plugs into the antenna wall socket. The noise occurs even when only the amp is turned on (and the receiver and TV remain in standby). It's an integrated amp and the noise increases in volume by turning the amp volume up. Turning the receiver volume up has no impact beyond the master volume. What's also strange is it only affects one of my amps. I have a secondary amp placed into the same position and with the exact same cables which doesn't generate this noise. The noise will immediate cease as soon as either the antenna cable or (either end of) the ARC HDMI is unplugged. I've found keeping the amp volume at around 30% makes the background noise barely noticeable, and I just have to turn my receiver volume up much higher to get to my normal listening volume. Doing this, constantly removing the TV antenna, or substituting my secondary amp isn't ideal in the longer term. Before I run out and start purchasing new cables to experiment with, has anyone else experienced this particular issue or have any solutions to preventing the antenna/cable seemingly being behind this noise?
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MIDDLETOWN - Looking to volunteer in the city for a couple of hours, or maybe overnight?
The Greater Middletown Interfaith Council's Warming Station at 58 West Main Street is hosting an orientation and training session Tuesday at 7 p.m. There will be another training hosted Dec. 11.
Having begun Sunday and going through April 2, from 9 p.m. to 7 a.m., the warming station will be open to host up to 30 homeless adults living on the street during the coldest months in the region.
Volunteers, men and women, are encouraged to stay overnight to supervise those who stay at the overnight shelter. All guests who utilize the shelter are screened beforehand for suitability.
Visit the warming station's website at www.middletownwarmingstation.com for more information on volunteering.


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My son's PT suggested just getting a cheap (or already owned old one) comforter cover and filling it with pillows and small couch cushions. Its a great challenge and is soft and safe built in!
Hi! My son is so eager to pull himself up to standing and my husband and I are trying to find quality yet affordable climbing toys for our son Max. The ones we've seen online are ridiculously priced!!! Any suggestions or alternatives are welcome. Thanks!!!
My son's PT suggested just getting a cheap (or already owned old one) comforter cover and filling it with pillows and small couch cushions. Its a great challenge and is soft and safe built in!
Hi Tamara... we bought our son a Step2 Kangaroo Climber when he was about that age, he was also very coordinated at 7 months... climbing up the couch and up on the furniture. He loved it, and now he's moved on to the regular playground down the street (he's 2 years 2 months). I'm going to sell climber, but if you're interested, just let me know. We bought it new for about $150 at Toys R' Us, we're gonna sell it for $50. Still in perfect condition.
Thanks! S.
Your appeal touched me enough to take a moment to write. Having studied with Magda Gerber, founder of Resources for Infant Educarers, RIE, and running my own programs for children under 5 for the past 30 years as well as raising my own two children I BELIEVE in simplicity want to share with you if you haven't heard of rie.org as a resource and guide.
You can create most everything your baby needs yourself and observation (which it sounds like you are good at already), respect, and allowing time for uninterrupted free will take you far. A friend and colleague of mine also has a site that might give you ideas or meet your needs.
Enjoy your Sunday and everyday of the journey ahead,
Baby Sitter's Club
Bueno Baby Furniture
Space-age highchairs and walkers with bells and whistles don't
sit well with you.Offer your munchkins the best seats in the
house with Bueno Baby furniture.
Resources for Infant Educarers instructor Hari Grebler started the company as an extension of the teaching method that believes children should develop at their own pace, but the stripped-down pine pieces have broad appeal.
Circular dining chairs are ideal for little tushies ready to sit on their own. Kidney-shaped tables let tots face teacher and one another with ease. An adorable village kitchen (available by special order) lets up to four cooks have at the soup.
Each piece is handcrafted and finished with nontoxic sealer.
In other words, it's all bueno.
Available by phone order ###-###-####) or e-mail [email protected]____.com see styles, go to harisriestudio.com.
I second the upside down laundry basket thing. A long ballet-like bar in a hallway can be fun for cruising. A leather ottoman (they have some a target) can be fun. We have an old trunk with leather handles that my young lady is enjoying. A well-secured bookcase with nothing on the bottom couple shelves (except things for baby) can be good. Step stools, benches, coffee table, building/carpeting something akin to a couple large steps... Have fun :)
I agree with the craigslist suggestion. Research the kinds you want then search craigslist.
Good luck,
Try Funandfunction.com
We bought a Kangaroo Climber for my grandkids at Sears. They are just an appliance store here in town but it's in their catalog and can get in in a few days. It was on sale for under $100.00 even with shipping. I had a similar one in my toddler room of my child care center and loved it. The kids could do different things on it at different ages. As young toddlers they could pull up to it and peek in the holes or crawl through the tunnels under it. They could play inside the little area, the bigger kids would use to climb up to the slide. Even older kids (4-5 yrs. old) still can use it because the weight limit is around 60 lbs.or more.
There is also the Koala Climber. Check out step2 stuff online and read the reveiws. It is a long lasting toy and we bought our Kangaroo Climber specifically to stay inside. But we also have Little Tykes stuff outside too, just bigger.
The kids in my daycare love this little car that they can stand up with. http://www.amazon.com/gp/product/B000NW3LZ6?ie=UTF8&t...
I have found it to be very durable and it has the added bonus of helping to walk.
Not quite sure about "climbing toys" but my lil girl loves to pull up on big boxes or an upside down laundry basket and she like it if i put a few toys or snacks on top so she gets all excited when she reaches them. use a big box though, like bigger than a diaper box, because if the base is small it may tip! good luck
Have you tried Craigslist? I see you live in LA. Parents often sell large items that are gently or barely used for a STEAL!! I know I do... :)
try thrift stores they are great if you live in the LA area I saw a great climbing toy at the Salvation Army in Santa Monica
Can you give some examples of what you mean by "climbing" toys?? When I think climbing toys I really think of things for children who are walking and climbing onto things like small toddler slides and such. Most babies learn to pull themselves to standing using chairs and couches first and then "cruise". The jumperoo was our favorite upright leg exercise type toy for our twins.
My kids loved the fisher price play wall & a activity table. They could pull up & cruise down it playing with different toys. Both my kids were early walkers at 8 months. Here's a link to a picture on Walmart's site.... http://www.walmart.com/ip/3933932 hope this helps you.
You don't need to have your child climb just yet (he will be climbing all over the furniture soon enough). You can create an obstacle course with things to climb under and over, a chair (under), a small box, a pillow, etc. We have those foam mats you can lay out on the ground, and we would put them together into triangle or square shape for my son to climb through (many fun games can be made up with those). He also loved to climb on/over us and his stuffed animals at that age.


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News Agencies | Updated on: 1 December 2017, 13:13 IST
The Supreme Court on 1 December decided to hear a plea seeking ban on the use and manufacture of firecrackers across the country.
The Apex Court issued a notice to the Centre and asked it to file its detailed response within four weeks.
Earlier on October 9, the apex court imposed a blanket ban on sale of firecrackers in the Delhi-NCR in the run-up of Diwali to check the alarming pollution level.
A three-judge bench of the apex court had restored its November 2016 order, banning sale of crackers in NCR and suspended its September 2017 order - which allowed limited sale but banned imports from other states.


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Post screenshots of your favorite MSGSapper routes in operation here in this thread. I would love to see what you have done with them and will be monitoring this thread!
Bob (MSGSapper)
Post screenshots of your favorite MSGSapper routes in operation here in this thread. I would love to see what you have done with them and will be monitoring this thread!
Bob (MSGSapper)
Hello Bob
I have all of your routes installed into TANE but, wainting for final SP1 release and installed it for posting screenshots from your wonderful routes.
Hi Bob, thanks for making some excellent routes. I have all but Watseka and Wilson Mills installed. I'd love to post screenshots but everytime I try to take one TANE (78667) crashes and screenie is corrupt. Maybe after SP1 is released it will work for me I love taking various trains on quickdrives and I am just starting to get into working with industries. Steep learning curve as I have only had TANE since September and not used a train sim since MSTS over 10 years ago.
The Loops
I love this route and thought I'd try substituting the billboard trees with some pay ware speed trees from UltraScenery, since I'd purchased the package. The following pictures are therefore of my personal 'doctored' (trees only) route. For information, substituting the speed trees had little if any impact on the frame rates on my mid-range system (iMac). Shadows are on 'low.'
Roscoe Snyder and Pacific Freight 1 Session
Last edited by Vostrail; February 1st, 2016 at 07:22 AM.
Cattaraugus Creek & Lake Erie
On this route, I again substituted the billboards with my payware UltraTrees. I won't lie; the fps did drop on this occasion - you've only got to look at the tree density to understand why. However, I still got playable frame rates and I think the autumn (fall) and winter textures look stunning. I have included a selection of screenshots from the seasonal freight sessions (split over 2 posts).
80413; Shadows 'Low'; DD 3000m
Here are screenshots from the Winter Freight session.
80413; Shadows 'Low'; DD 3000m
Very pleased to see you enjoy them and by all means ANY SPEEDTREE in that volume will hurt even a 980ti GPU.
The Hump Yard I am building has plenty of them and I am around 45 to 50fps until I fill the yards with heavy poly third party rolling stock then I hit the floor at 13fps
Last edited by mcguirel; February 9th, 2016 at 10:13 AM.


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July 16, 2019
Dear Upper School Families,
By this time, you should have received in the mail a list of scheduled classes for the upcoming school year. This is an announcement that Columbus Academy's
online bookstore is now open
. Please note that until midnight on
July 23
, all orders of $99 or more will receive free shipping.
If you are interested in purchasing used books, the sooner you go online, the more likely those books will be available to you. New books are guaranteed to be available by MBS Direct, our institution's official textbook provider.
To order textbooks and course materials with convenience and accuracy, follow these steps:
Access our Online Bookstore at
Select the courses in which your upper school student is enrolled.
Select new or used books. (Messages on the ordering site will indicate if the teacher requires the book to be new. Some messages will indicate that books will be provided by the instructor or that no book is required.)
Enter your mailing address and credit card information.
Please place your order by
August 12
to ensure that you will have your books for the opening day of school.
In keeping with the commitment to money-savings, MBS Direct has developed services designed specifically for our school:
on textbook orders of $99 or more during the
first week
of sales from
Tuesday, July 16, through Tuesday, July 23
(free shipping for "out of stock" items still applies if you order those items now but select for your entire order to be shipped together once all of it is ready).
Customer Loyalty Program
-- when you buy from CA's online bookstore and choose to sell your books back to MBS, you will receive top-dollar for each book that is in-demand, plus an additional buyback bonus for being a loyal customer.
MBS has once again partnered with
Barnes & Noble Marketplace
to make shopping for used books from third-party sellers more accessible and convenient.
Columbus Academy chose MBS Direct to provide you with competitive prices for textbooks, the highest level of service, and because they can guarantee you the correct book every time you order. You can choose other online ordering options, but you must check MBS to find out what books are needed for each class.
to access
Columbus Academy's Online Bookstore
Questions or concerns about the ordering process, or any other product and service MBS Direct offers, should be directed to the MBS Direct 24-7 customer service team at 1-800-325-3252 or
. If you prefer to speak with the person at Columbus Academy who handles the book-ordering process, you can reach
Jennifer Lange
at 614-509-2281.


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Little mental case tyranny promoter can go straight to hell.
Clearly a death threat. Ban her from the USA, even if she wants to visit the UN.
[Time's "Scold of the Year," Greta Thunberg, told a crowd in Italy that "we will make sure we put world leaders against the wall" if they do not do what she tells them to do.]
Imagine how many followers Greta would have if she had a large round Kardashian buttocks?


text: I was at REI in Missoula, MT, one day and noticed several lights outside pointed downwards towards some tall grasses planted against the wall. Intrigued, I returned after dark to take some photos. Notice the downward facing lights along the wall. The lamp in the parking lot is extremely bright, but faces downward. The interior


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Daily Campus Tours
Campus Tour and Admissions Overview:
Join a small group of other visiting families and take a walking tour of campus with one of our student Rambassadors. You will also have the opportunity to individually meet with one of our Admissions Counselors and learn more about Jefferson.
Campus tours are available Monday through Friday at the below times. Depending on the number of families visiting each day, we may have a small Group Admissions Information Session rather than individual meetings with an Admissions Counselor.
Monday through Friday:
9:30 am
12:30 pm
2:30 pm


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4 SEO tools for all companies
Knowing all the SEO entries and exits is very important if you want to increase your rating and increase search performance for your website. Knowing the basics of SEO, and the tools that industry professionals use, enable you to grow your business to a great degree. Here the best SEO Company in Canada and the… Read More »


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Roll over image to zoom
Double tap image to zoom
Blue Buffalo Blue Freedom Grain-Free Indoor Weight Control Chicken Recipe Dry Cat Food
Sold in stores only. Selection varies by store. Check your local store for availability.
Blue Buffalo Blue Freedom Grain-Free Indoor W...
35% off your first order & free shipping on $35 when you select Repeat Delivery in cart!
10% off – See Price in Cart! Curbside pickup now available in most locations.
35% off your first order!
- 5% off and free shipping on all subsequent orders.
- FREE shipping on $35 on your first order.
- No commitments. Pause or cancel at anytime.
Attributes / Specifications
Ingredients & Guaranteed Analysis
Additional Details
For pet parents who want to feed their furry friend a diet without grains, there's nothing better than Blue Freedom. It's free from the grains and glutens that can trigger allergic reactions in some cats. Made with the finest natural ingredients, Blue Freedom features:
- Delicious, protein-rich, Deboned Chicken
- Whole carrots, sweet potatoes and peas
- Blueberries and cranberries
- NO chicken (or poultry) by-product meals, NO corn, wheat or soy and nothing artificial
- The ingredients in Blue Freedom are chosen with great care by our animal nutritionists, and formulated to help your cat achieve and maintain a healthy body weight.
- Primary BrandBlue Buffalo
- Primary FlavorChicken
- Weight11 LBS
- LifestageAdult
- Days to ShipShips Next Business Day
Some cats just don't do well with grain-based foods. That's why we created Blue Freedom dry food for indoor cats. Blue Freedom contains no grains, so it has no glutens. Glutens can trigger allergic reactions in some cats. Blue Freedom Weight Control for indoor cats is formulated to meet their special needs:The carefully balanced calories in our proteins and fat help maintain an ideal, healthy body weight The right blend of beneficial natural dietary fibers help push hairballs through the digestion process and minimize litter box odor High-quality protein from delicious deboned chicken, plus L-Carnitine, helps cats stay lean


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Vincennes • Cabinet de curiosité
The augmented reality serving the Architectural Heritage.
Objectives :
Show to the visitors a 3D reconstruction of the office's organization of Charles V in the fourteenth century, in augmented reality.
Solution :
Augmented reality device running on Android tablet and available to the public in the Château de Vincennes.
The compass and the accelerometer are used in conjunction with the Augmented Reality to allow reliable positioning and reduce the number of markers.
Technologies :
The application developed with Unity 3D uses Vuforia SDK for augmented reality and runs on an Android tablet.
Demo / Vidéo :
Video presentation : Click here


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630-922-5799 | 555 Weston Ridge Dr
Wheels and Tires
Wheels and Tires, Naperville, IL
High-Performance Tires and Wheels
Stop by Frankie & Johnnie's Garage at 555 Weston Ridge Dr for new tires and wheels and go from ordinary to extraordinary in seconds. If you like cruising or racing, a new set of tires for your high-performance car or truck will keep you going mile after mile. Grip the turns on Naperville roads, or win on the track with the right tire and wheel set.
From all-season radial tires to super sticky drag slicks to monster truck tires, we have the right tires for your driving application. Add wheels in a variety of finishes from chrome to black matte and you have a winning combination. Get your new tires wheel-mounted, balanced, and ready to race at Frankie & Johnnie's Garage in Naperville. Give us a call at 630-922-5799 and we'll keep you rolling!
Top Tire Brands
Top Wheel Brands
- Weld Racing
- American Force Wheels
- RC Components
- Rolling Big Power
- American Racing
- Fuel Off-Road
- Aero Race Wheels
- U.S. Wheel
- Vision Wheel
- Billet Specialties
- Forgeline Motorsports
$5.00 OFF Oil Changeview coupon
Mention our website and bring in this coupon to save on your next oil change.
Cathy A.
Excellent work! My whole family goes to get their cars repaired. They are reliable and reasonable. I recommend them!


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People who are blind or have low vision, with the right training and accommodations, can do any job that fits their particular interests, skills and abilities.
If you are blind or have low vision and would like assistance in finding or keeping a job, we can provide you with:
- Job Counseling
- Skills of Blindness Training
- Assistive Technology
- School-to-Work Transition (Age 14 to 21)
- Request Services
Job Counseling
What types of jobs can people who are blind or have low vision do?
People who are blind or have low vision, with the right training and accommodations, can do any job that fits their particular interests, skills and abilities.
How can DSB help me find or keep a job?
We are experts in providing services for people who are blind or have low vision to help them find or keep employment.
Our counselors and staff:
- Assist participants in exploring a wide range of possibilities for employment.
- Empower and teach participants to educate themselves about careers and the job market.
- Help participants identify what tools or skills they may need to prepare for the career of their choice.
- Support and encourage participants as they learn skills to overcome barriers, regain self-confidence and obtain or maintain employment.
In addition, we can work with you to explore opportunities for self-employment through either your own business or through our Business Enterprise Program (BEP).
Skills of Blindness Training
People who are blind and have low vision use adaptive techniques and skills to accommodate vision loss. These "skills of blindness" include using Braille and specialized computer software, traveling with a white cane, and cooking, cleaning and sewing techniques. Adaptive skills enable people with vision loss to be independent and successful in the home, in school, on the job, and in their communities. We provide training in the skills of blindness:
At Home
We provide daily living skills training to all adults, both those interested and not interested in employment, in their homes and in their communities.
If you are an adult who is blind or has low vision, and would like to learn adaptive skills in your home and in your community, DSB can provide assessments and training at no cost to you.
What can DSB do for me in my home and community?
We can:
- Provide counseling regarding living and/or working with vision loss.
- Determine what skills, technology and resources you can use to help maximize your strengths.
- Provide information on national, state, or community resources.
- Teach adaptive skills for reading and writing, home management, computer use, and the ability to get from place to place.
- Recommend adaptive devices for use at home, in the community, and/or on the job.
Is there a difference between the services provided for people who are looking for employment and those who are not?
We plan and provide services based on the individual. People who are blind or have low vision and looking for employment will typically receive more in-depth counseling, assessments and training, which includes vocational planning, to determine how they can enter or continue to thrive in the workforce. People not interested in employment also receive services but typically within the scope of daily living.
At an Intensive Training Center
If you are an adult interested in employment, you may qualify for intensive learning at our Orientation and Training Center (OTC).
Assistive Technology
What is Assistive Technology and Accommodation?
Examples of typical AT devices or accommodations for someone who is blind or has low vision are:
- Software to magnify a computer-screen view or to have what appears on the computer screen voiced or Brailled
- Handheld or desktop electronic magnifiers to see small print
- Devices to scan and voice a printed document, flier, or book
- Changes in lighting to reduce glare or increase visibility
- Adjustments to a workstation layout to improve workflow & ergonomics
What can DSB do for my AT needs?
DSB's AT Specialists help people who are blind or visually impaired acquire and use appropriate AT to help them participate in activities of daily living, employment and education.
DSB clients, along with their teams of VRCs, Rehab Techs, Rehab Teachers and Low Vision Specialists, and AT Specialists, decide which type of assistive technology would be most helpful for the client's career goals. The team is trained to match particular technologies to specific needs to help the person function better or more independently.
Our AT Specialists
- Offer with assistance in the selection, acquisition, and use of assistive technology devices
- Provide training or technical assistance
- Our AT Specialists also stay up to date on the latest products on the market that will benefit their clients.
What if I'm not going back to work?
The Department of Services for the Blind's Independent Living (IL) Services IL Services can help clients find the right combination of AT aids, devices, and accommodations to help clients regain the desired level of independence in their homes or communities.
Learn more about DSB Independent Living Services and available aids and devices.
School-to-Work Transition (Age 14 to 21)
School-to-Work Transition helps students, as young as age 14, and their families think about and plan for life after high school. DSB counselors assist with this transition by:
- Consulting on the student's vocational activities including part-time employment and internships
- Participating in Individual Education Plan meetings
- Providing information regarding our services for adults, including job counseling and training in the adaptive skills of blindness
- Working in collaboration with students, parents, and school staff on post-school activities
What are the parents and students' responsibilities?
Until the student is 18 years old, a parent or legal guardian will sign to authorize services. Parental involvement and support is very important, especially in the early part of the process. Our goal is to move the student toward greater independence. Parents and students are asked to attend meetings with the DSB counselor and school staff to work in collaboration on post-school activities.
How long do DSB services last?
There is no set time limit. Time varies depending on the individual needs of the client and the services provided. Once the student has reached the age of 18, he/she can continue to receive services in:
- Job Counseling and Placement
- Skills of Blindness Training
- Assistive Technology
What can DSB do for children, their families and their service providers?
Department of Services for the Blind (DSB) serves children and youth who are blind or have low vision from birth through age 21.
We provide:
- Information and referral to the appropriate community resources.
- Training in understanding the specialized developmental needs and parenting techniques for a child who is blind or has low vision.
- Assistance in understanding the nature and potential impact of the child's visual disability.
- Counseling towards building the foundation of a productive, independent and successful childhood, youth, and adulthood.
- Assistance in advocating for access needs through the K-through-12 school system.
- Training for the child and their family with daily living skills to help the child become more independent at home and participate in household chores.
Request Services
Who is eligible for services?
If you want to be employed and are blind or have low vision, you may be eligible for services. Read about service eligibility.
Are there any fees for your services?
No. We maximize federal and state resources to keep our services at no-cost to you.
How do I apply?
Request services online for yourself or a loved one. Or contact us at 800-552-7103 or email@example.com for more information.


text:
Communication - brochure
Intense mediterranean rains : 8 measures to adopt
intense rains - 8 measures to adopt (format pdf - 1.5 Mo - 14/12/2018)
Floods : safety precautions while driving
Be careful, your car could be a dangerous trap !
safety precautions while driving (format pdf - 647.1 ko - 27/12/2018)
Floods : be careful while the basement is flooded
On the Mediterranean arc, the phenomena of intense rain and the associated floods invite us to anticipate and better prepare ourselves to cope with ever more frequent and faster episodes.
In the Alpes-Maritimes, in October 2015, about ten people drowned in underground car parks. These major events have highlighted that in case of flood, an underground car park can be a deadly trap.
Based on this observation, recommendations for users and managers of underground car parks have been formalized through a dedicated brochure developed by the interregional mission for the coordination of flood risk prevention on the Mediterranean arc. DREAL PACA. This project was carried out in collaboration with Cerema and Entente de Valabre in association with the key players of the "Mediterranean arc" territory.
The Mediterranean arc being a particularly tourist territory, that's why this English version of the brochure was produced. A poster was also created.
poster parkings EN (format pdf - 1.4 Mo - 14/12/2018)
Special video effects applied for flood prevention
The report shows us that the common population does not have a very clear but an abstract idea while dealing the risks of flood with maps and general informations.
The need to develop new tools such as photos, virtual reality … is strong. Such devices would be usefull to help them to better unterstand the real risk and the possibilities of various catastrophic situations (for example : the height, the rapidity, the direction and the effects of the floods).
The simulators could be an effective tool to feel the physical sensation in case of flood. Some simulation can involve them to become aware of risks and to adopt good behaviors and measures in case of flood.
Another way is to create virtual reality in using every day life situations. It was the aim of the partenarial project which was put up by the interregional mission for flood prevention in the Mediterranean Arc, the river syndicate of Argens and Pierre SCHOLL.
The project included the experimentation of the application of special video effects to a film produced to a normal time (without floods).
The results of the project are very realistic and usefull for an efficient sensibilisation of the population.
Even if this technique presents some constraints (cost, duration…) and cannot be used for all situations, the use of special video effects is very appropriate and efficient for the territories where :
- the last floods have an historical date,
- the risk awareness of the population has to be developed,
- the water flows are complex and difficult to apprehend,
- the work project needs to be accompanied with efficient communication system,
- …
You can see the result on this page.
Tsunami Risk
The Mediterranean arc, coastal region, could be exposed to tsunami generated by strong earthquakes off the coast of France, Italy and Maghreb. The beaches, the ports and the urban zones closest to the sea could be impacted.
The downloadable document below gives some advice for the exposed population to react quickly.
Tsunami risk : good manners to adopt (format pdf - 501.4 ko - 26/11/2018)
Télécharger :
- FLOODING - BE CAREFUL WHILE THE UNDERGROUND PARKING IS FLOODED (format pdf - 858.3 ko - 14/12/2018)


text:
Copywriting Samples
Need an experienced health and medical writer to produce your next lot of health content?
I can help you with SEO optimised blogs or web copy, well-researched and credible health and medical articles, patient information sheets, health content planning or business branding for health organisations.
But don't just take my word for it. Have a look at some of my copywriting samples.
Health and medical writing
Immunotherapy cancer treatments: the European perspective, published in The Mandarin, 10 October 2018
Laser eye surgery – what you need to know, NewVision Clinics
Blog writing
Over-the-counter pain medication: everything you need to know, Bupa, October 2016
What does your family medical tree look like?, Defence Health, July 2017
How does climate change affect life's luxuries?, The Clean Collective, April 2019
Web Copy
Sarah Hamilton Birth and Postpartum Support
Online Magazine
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text: Aktivehate - My Own God (CYGNOSIC vs AKTIVEHATE MIX) by aktivehate published on 2013-04-02T18:51:07Z - Right before going away to the Cygnate (Cygnosic + aktivehate) Russian tour, the aktivehate facebook page hit 2000 likes and I said I would find something to "celebrate" that. - During the aforementioned tour, Georg (Cygnosic) and me messed around with My Own God for fun, and it ended up sounding pretty cool. Ok, so I decided to do 1+1 and just release this great remix/rework/collab for free download to celebrate breaking the 2000 likes barrier. Remember to show some love to my bro Georg too, over at www.facebook.com/cygnosic <3 Genre Aggrotech / Electro-industrial Comment by Ellen Phipps This is such a lovely track 2017-03-07T09:58:07Z Comment by Virtual Walken Great!!! 2014-11-10T06:29:56Z Comment by eDGe ...I'd just ordered the album... great !!!! 2013-09-29T13:52:05Z Comment by eskarina evilredhood verynice ♥♥♥ 2013-04-04T06:15:03Z Comment by lezsbika Nice feeling 2013-04-03T20:31:33Z Comment by Devils Breed [Voxar] amazing track in a great remix, impressive match! 2013-04-02T22:38:10Z


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The Pubwatch Star Award, sponsored by Licensing Defence Barristers, has been awarded to PC Rob Sparkes.
Thanks to a project conceived by PC Rob Sparkes, offenders who have assaulted or abused people working in the night-time economy, such as taxi marshals and door now face the prospect of being banned by the licensees participating in the Bedsafe scheme.
Rob was joined by his family at a private ceremony in, and was presented his certificate by Chief Constable Garry Forsyth and the regional representative for the National Pubwatch, Steve Evans. PC Sparkes was recognised for his sterling service and outstanding contribution to the success of the Bedford Pubwatch scheme.
PC Sparkes said: "I am proud to have received this award; it was very much a team effort and I would like to thank my BedSafe colleagues for supporting the initiative.
"I would also like to thank the force and the Police and Crime Commissioner for their support. The initiative means that night time workers in Bedford are protected from those individuals who commit offences against them."
Stephen McCaffrey, lead barrister at Licensing Defence Barristers, congratulated PC Sparkes saying:
"Licensing Defence Barristers is pleased to be sponsors of the National Pubwatch Regional Star Award. Pubwatch makes a valuable contribution to maintaining high standards in the sector by promoting best practice around the country.
"I would like to personally congratulate PC Sparkes on receiving the award which is well deserved."
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General Description
Ideally located on the beach front of Torremolinos in the Costa del Sol area of southern Spain, Hotel Puente Real brings you the best of Andalusian hospitality with top-class services and amenities. The hotel has 371 ultra-comfortable rooms and 102 Andalusian-styled apartments with gorgeous sea views. The apartments are well-appointed with spacious bedrooms, living quarters, en-suite bathrooms. The in-house restaurants of the hotel also offer stunning sea-views, alongside scrumptious buffet spreads and live cooking shows. There are two expansive swimming pools to cool off, while separate swimming areas for kids keep them happily busy. With pool bars, mini-clubs, indoor cafeterias, TV areas and daily day and night entertainment for adults and children, the hotel will make sure that you have a great time on your holiday. There are also private parking and WiFi, both of which can be used with a small fee. The resort is great for outdoor sports such as windsurfing, sailing, kite surfing and diving. If you are looking for family-friendly fun and affordable luxury in Costa del Sol, Hotel Puente Real is your go-to place.
The hotel is located right at the beach front of Torremolinos in Costa del Sol. It is quite near the Malaga airport in Costa del Sol, which is located at a distance of 4.2 km. It takes around 8 minutes to drive down to the hotel from the airport. The famous beach of Playa de Guadalmar is located at a distance of 8.6 km.
Room Types
All the rooms in hotel are well-appointed with a cool Mediterranean vibe. Decorated in the shades of blue and white, the rooms come in three types: superior rooms, double rooms and apartments. The superior and double rooms have stunning sea-views with a host of facilities such as en-suite bathroom, TV, hair dryer, safe, refrigerator and WiFi facilities. Guests are also served with water and wine on arrival. The apartments have, in addition, sofa-beds, living area, and plenty of other features.
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The main restaurant of the hotel serves lavish buffet spreads featuring an array of local dishes and international fare. Restaurant Italiano La Farfalla offers the best of Italian cuisine, while the pool bar serves delicious cocktails right at the pool area. Hall Café serves freshly brewed coffee and tea during the afternoon.
Sports, Games and Entertainment
The beach side is definitely the main attraction of the hotel. The beachfront of Torremolinos has a great tourist vibe and there are small shacks, famous for serving delicious snacks. While the adults are having all the fun, the hotel makes sure that the children are busy as well. There are regular shows and a kids' club for your little ones. For the active souls there is a large fitness centre with cardio machines and free weights. The hotel also has two adult pools and a children's pool. Sport lovers can practice golf and enjoy a range of water sports at the beach front. The hotel features tennis courts, multi-sports court, petanque, football, archery, volleyball, darts, aerobics and aqua gym. The neighborhood of Marbella offers plenty of shopping and dining options. The old town of Malaga has great museums, old buildings and art exhibitions that can be enjoyed at leisure
Check-in from 2 pm
Check-out until 12 pm
Children are welcome
Pets not allowed
All major credit cards are accepted
Other Useful Info
Distance from Malaga airport: 4.2 km
Distance from Playa de Guadalmar: 8.6 km
Please take note of following link to be sent to all our mutual guests so they can proceed to the check in online :


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Puducherry, Aug 2 (PTI) Puducherry Chief Minister V Narayanasamy on Sunday said the territorial government would soon open a hospital to offer treatment under the Siddha system of medicine for coronavirus patients.
The Siddha method of treatment has been in vogue in neighbouring Tamil Nadu and a number of patients were being treated and cured of the infection, he said addressing a virtual press conference.
'The Puducherry government too would introduce the Siddha system of treatment and those willing to take treatment under this indigenous medical system could opt for it,' he said.
A team of doctors from Puducherry would visit Siddha hospitals in Tamil Nadu to acquaint themselves with the various modalities and features to offer the treatment under Siddha system to patients here, he added.
Stating that the occurrence of the COVID-19 in East Godavari district in Andhra Pradesh was impacting the Yanam region, an enclave of Puducherry, the Chief Minister said Health Minister Malladi Krishna Rao, the local MLA, and Health Secretary T Arun had been camping there to strengthen the infrastructure and look into other issues.
The chief minister said the government had increased testing in rural areas and AYUSH care hospitals in the Central and state sectors here would also strengthen the medical care facilities for COVID-19 patients.
Narayanasamy had earlier visited various vegetable and fish markets and shopping centres in and around Puducherry and advised merchants and shoppers to adhere to safety norms particularly to maintain social distance and wear masks.PTI CORR SS PTI PTI


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Let's just clear something from the oft; I've never really been a fan of the Arctic Monkeys. It's not them as a band whatsoever (I mean I Bet You Look Good On The Dancefloor was actually a pretty solid track), I've just never really 'got' the rock/indie genre. When the group were headlining the Pyramid Stage at Glastonbury earlier this year, I was shovelling the funk and getting into the disco spirit with Chic over at West Holts; there was just very little interest from my side to focus on guitar bands (even though, ironically, part of Chic/Nile Rodger's prowess lies on the quality of the strings).
However, over the past few months, my ears have been opened and I've started to warm with what the rock bands are doing now, especially their trial of fusing different genres. When I'd heard that the inspiration for the Arctic Monkeys' new album AM was driven by Dr Dre and Aaliyah, I thought I'd throw my ignorance out the window, take a sit down and listen. I'm happy I did.
Recent single Why'd You Only Call Me When You're High? is one of the clear contenders of Dre's influence. Arguably the catchiest song on the album, not only does the track seem to pay homage to the 90's 'G-funk era', but there's clear weighting toward the Doctor's What's the Difference?.
Opener Do I Wanna Know? soothes the listener into the album and introduces this fairly new direction from the band; guitarist Jamie Cook's slow, melancholic riffs massively compliment Turner's eerie vocals in the bridge – at 4:32 it's by no means a short track, but you'll definitely wish it continued on for longer.
R U Mine, the track originally leaked to confusion (yet fanfare) over 18 months ago, makes a welcome appearance on AM, and will particularly please Arctic Monkeys' fans of old, whilst there's something intriguing by One For The Road – you could pick out some form of The Weeknd influences here, and by the end of the track you'll be 'crowing' and 'woo-woo'ing' akin to the Rolling Stones' Sympathy For The Devil.
Arabella seems to take strong hues from AC/DC's Back In Black, whilst Turner's lyrics show no holding back here with "….and a helter skelter 'round her little finger and I ride it endlessly…". It's glam rock, with 70's funk and just seems to work effortlessly. Meanwhile, the melancholic No. 1 Party Anthem (which is as much a number one party anthem as One Direction's Best Song Ever is the best song ever) seems to dip in to Radiohead's No Surprises instrumental; it's by no means a tub-thumping track, moreso a poignant ballad dominated by Turner's array of pitches.
Snap Out Of It, whilst by no means the strongest song on AM, will get the crowds moving, with Turner's muffled tones along with a wondrous beat providing the perfect toe-tapping background that is sure to help the British band win over new fans.
With only 12 songs, AM is a fairly short album, but its influences from the 'history-of-good-music' are apparent. It seems Turner and his cohorts understand the values of cross-genre, and whilst they will always be a band with their roots sown in to rock, the group aren't afraid of trialling new avenues. It's an incredibly satisfying album, and one which not only captures upon first listen but also grows after play number 2, 3 and 4.
Will I forego Chic for Arctic Monkeys next time there's a festival clash? Probably not….
Will I be an eager fanboy when the band support the album on tour? Again, probably not.
Will I be listening to this album again and preordering the follow up? At this rate, definitely.
I may not have been a fan to begin with, but the Arctic Monkeys have definitely won me over with AM. It's been an experience I couldn't have prepared for, and I'm now left wanting more.


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The citation requiring your bank to have a Bank Secrecy Act training program is FDIC Regs, 12 CFR 326.8(c). It calls for "training for appropriate personnel."
There is no regulatory language that defines "appropriate personnel" as including lenders. However, I suggest you read some of the background information on the AmSouth civil penalties. The lending function in that bank was implicated.
In addition, the material put out by FinCEN in its SAR Reviews shows that a fair amount of suspicious activity involves money laundering via loans, as well as increased instances of mortgage fraud.
No one is suggesting that lenders should have to sit through classes on completing CTRs! That would be the height of folly. But lenders often know and see things about bank customers that no one else does. When those things are suspicious, the lenders need to be aware of the requirement for filing SARs, and the bank's processes for getting that done.
As for your Compliance Officer, this wake-up of your lenders should make the CO an ally, rather than an enemy.
For what it's worth, I'd recommend writing your finding up as a strong recommendation, and include it in the report (not just the exit interview). Make certain that senior management gets put on notice. You don't want your audit of BSA compliance to be found wanting by your examiners when they next arrive.
First published on BankersOnline.com 1/31/05
BSA Training Is For Everyone!
The citation requiring your bank to have a Bank Secrecy Act training program is FDIC Regs, 12 CFR 326.8(c). It calls for "training for appropriate personnel."


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On Sunday the 1st of December 2019, Nash took to Twitter to announce the release of the Nash App on Android Devices. The Nash Mobile App will now allow Users to manage their account similarly to how they manage it on the web version, featuring the ability to send, receive and manage their digital assets on the go with full access to the Ethereum, NEO and Bitcoin products offered on the platform.
Nash has been quietly building out and updating their Nash Exchange since it launched back in September of 2019. With Bitcoin and over 1,200 Ethereum ERC20 tokens now supported on the platform, the Team is building what in our opinion is one of the most user-friendly products built on the blockchain today. As more states in the US have more access to Nash with the most recent addition being California, the gateway for institutional money to flow into the industry is opening wider every day. Certain US states are extremely strict with what they'll allow their citizens to invest in or access when it comes to financial services and products, but Nash appears to be playing by the rule book and taking those small steps to ensure they're compliant with state regulations and available to as many Customers as possible.
The Nash Mobile App handles just as expected with a clean, fluid experience allowing you to keep track of your digital assets and any assets you may be interested in, as well as allow you to send and receive funds with near-instant transaction speeds thanks to the NEO Blockchain Nash is built on. Nash announced the Android version alongside a "coming soon" announcement for the iOS version. The Apple App Store has been a pain in the side for a lot of blockchain projects due to their strict approval process which has caused other projects like BOLT and their Pegasus Wallet to be delayed on iOS for several months as a result. Users may not have to wait long though as Nash is clearly working with regulators all the time and tweaking their product to adhere to strict guidelines with every update.
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Chris and his helper did a good job of getting the yard ready for a regular mowing. However... it took the two of them 35 minutes to complete and my bill is $243. I know I approved it ahead of time but that's a pretty high hourly rate. Can we cut that in half? Chris mentioned as he was leaving that he thought the yard was larger than it turned out to be and to see if I could work something out. Thanks!
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