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Do you like to be manipulated? No one does, but I'm paying to be manipulated on December 22nd. Well, elbow manipulated anyway. Don't elbow manipulate me, or do, because I need to be fixed. Ever see a manual elbow manipulation?
If you've been following along at all, you probably know that I'm quite broken. But the thing is, although I've been going on with my life as if I didn't have all of this hardware in my broken fin, I'm really not getting a whole lot better. In fact, I've kind of stopped swimming in a proverbial sense.
And now, in a literal sense in more ways than one, I'm frozen. Progress is no longer a thing. My supronation is at 0º and pronation at about 5. I can bend my elbow at about a 40, straighten at about a 60. And that's it. Not cool.
I've kind of stopped swimming altogether and to be honest, my psyche is beginning to completely drown with my broken fin. Typing is a challenge, photography is a huge challenge, cooking's a challenge, driving is a challenge, even grocery shopping and putting away said groceries is a challenge. Life…is a challenge and it totally sucks.
I know I don't have it as bad as many people do, I don't mean to sound whiny (although I know I totally do). It's just that I've grown tired of this broken fin and I'm ready for it to be healed. It should be healed. A badass reconstructive surgery and three month of intensive physical therapy, it should be healed dammit.
But alas, all of that hardware in my arm, the necessity for the placement of that hardware, has caused my body and my brain to decide that my arm just shouldn't heal. That tendon is so tight, it could probably hold a small child as they zip line. I exaggerate not. It's just…so…frustrating!
On December 9th I headed to my ortho appointment for my 12-week follow up. The physical therapists recommended a brace that would surely begin my transformation into an official robocop, or robomom, or something. Whatever. I was ready for the bad news and hopeful that the ridiculously cumbersome apparatus would let me gain at least a little of that supronation and pronation back. I really need those things…badly.
In a strange turn of events, my ortho looked at me calmly and said, "We need to do a manipulation. We need to knock you out and manually manipulate your arm and elbow. We need to get that thing moving again.".
Of course we do! But, wait. WHAT does that mean? Knock me out? Manipulate? Like move this broken bitch around, manipulate? And so, the outside voice questions started pouring out of my mouth…
"So, you're going to put me under and move my arm ridiculously in places it doesn't move? Is that what you're saying?"
"That's going to be painful, right?"
Well, you'll be asleep, but yes, it would hurt like a bitch if you were awake. Like you'd be throwing up, crapping your pants, and passing out if you were awake, but you won't be."
"What about when I wake up?"
There will be plenty of narcotics involved. And it's super important that you're back in therapy the next day to keep it moving so it doesn't freeze back up.
"In therapy? The next day?"
Yes, with narcotics. It will be painful for sure, but you'll be moving again.
"OK, sounds good. But the risks? What are the risks? There's always a risk."
About 5% of a re-break. Well…in the perfect world…with someone with strong, adult-sized bones (<~~~none of these apply to my tiny, fragile bones)…it's about 5%. But we'll be monitoring that and we'd know right away. It'll be fine and you'll be moving again.
"I really do want to move again. It'll be fine. I'll be swimming again in no time."
And so, this manual elbow manipulation was scheduled for the week of Christmas. Joy to the world. I can't say I'm overly excited, or overly anxious, or overly scared, or overly anything in fact. Possibly overwhelmed, but not overly emotional in general. What will be, will be, and I'm hopeful that I'll get a good bit of my range of motion back. I'm hopeful that I'll get my life back!
If you're curious about the surgery, watch the video below. It's not in English, Bug and I have no clue why they are laughing, but I've probably watched it 100 times
because I'm sick like that because I need to feel like I have some sort of control over the situation, at least by being in the know. Shit looks like it's going to hurt considering I can't bend it at all, but as they say…No Pain, No Gain! PS – I'm certain my doctor is way better at it than this.
Wish me luck, send sparkles, talk to the man upstairs, or whatever it is you do please and thank you. Here's hoping I'll be doing swimmingly in the coming weeks. Hold the narcotics.


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There is new legislation that has farmers and tailgate markets across North Carolina very concerned. Though there is very little information on the legislation at this point, ASAP is hearing from markets that they are already losing vendors and managers because they cannot or do not want to be responsible for this new regulatory burden. N.C.G.S. § 66-255 has the potential to create an insurmountable barrier for many new markets, it threatens the national leadership that North Carolina has shown in providing outlets for farmers to direct sell their products, and it undermines the great progress the state has made in expanding access to food insecure communities.
ASAP | From Here | Appalachian Sustainable Agriculture Project
Thousands of miles fresher
Comment. Share. Connect. Join ASAP in an ongoing conversation about local food FROM HERE in the Southern Appalachians.
Jan 27, 2014
New NC Legislation (N.C.G.S. § 66-255) to Impact Farmers Markets
Jan 22, 2014
Fresh at (Winter) Markets This Week
Despite snow on the ground, shoppers are getting out to winter farmers tailgate markets! Word on the street is that if you want to get kale, you need to get there early as produce vendors are selling out fast! In addition to kale, look for turnips, carrots, Kohlrabi, sweet potatoes, Daikon radishes, winter squash, baked goods, meats, cheeses, eggs, body care products, and more.
Jan 14, 2014
Fresh at (Winter) Markets This Week
It's like spring in the winter! Not necessarily for temperatures, but certainly if you're shopping at winter farmers tailgate markets. Lettuce, kale, turnips, winter squash, and Daikon radishes were all spotted at markets this weekend. In addition, you could also find market staples such as local meat, cheeses, potatoes, eggs, sweet potatoes, and more!
Jan 07, 2014
Fresh at (Winter) Markets This Week
Winter farmers tailgate markets are heating up! This Saturday marks the second Woodfin YMCA Indoor Winter Tailgate Market (10 am-12:30 pm). The first market was a big success. New Moon Herbs Farm's produce included sweet potatoes, kale, and lettuce. Ivy Creek Family Farm had turnips, mushrooms, arugula, and more. Cheese vendors like Three Graces Dairy and Blue Ridge Mountain Creamery offered up their specialties. There were apples, ginger, greens, carrots, sprouts/microgreens, meats, crafts, cheese, and baked goods.
Aug 08, 2013
Program Post: A Look at Asheville City Market
Each Thursday, ASAP's programs take you behind the scenes of their work. This week, our Local Food Campaign talks about Asheville City Market (did you know it's an ASAP program?!) and their excitement about Saturday's Tomato Festival.
Jun 13, 2013
Program Post: Go West! An ASAP Road Trip
Each Thursday, ASAP's programs take you behind the scenes of their work. This week, our Local Food Campaign talks about their recent trip out west—the far west of WNC, that is—to spread the ASAP gospel!
May 07, 2013
Follow the (Local) Cheese…
…to area Appalachian Grown™ partner tailgates and restaurants this May!
Apr 29, 2013
Fresh at Farmers Markets This Week
Adore asparagus? Then get thee to a farmers tailgate market this week and weekend! Although stalks have been trickling in for a bit, markets are moving toward asparagus abundance thanks to vendors like McConnell Farms (Asheville City Market, Asheville City Market South, North Asheville Tailgate Market, West Asheville Tailgate Market).
Apr 16, 2013
Local Food On Film!
ASAP's spring communications intern, Casey Toth, is at it again! She has created another great video. This time, she combined still images with video interviews to tell the story of local food in Western North Carolina and the Southern Appalachians—particularly in Asheville—as she's experienced the movement so far through her internship. The interviews feature folks behind artisan food products and Appalachian Grown™ partner restaurants, including Green River Picklers, HomeGrown, and Posana Cafe. If you see her out snapping photos or filming at spring tailgate markets, be sure to say hello, comment, and, of course, smile!
Yes, it is spring. One way to know for sure, even with this week's confusing cold and snow? Tailgate tents are going up, and area farmers markets are opening outdoors for the harvest season!

subset: commoncrawl


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Hat Silk Plum
US$29.02 Inc VAT
Beautiful! Matches my base layer perfectly! My horse has the pad and fly hood, and I'll be wearing this. Bring on summer!
Date Added: 07/02/2020 by Karen Barker
Nice silk, quality product, bobble is great, recommend
Date Added: 22/10/2018 by Kendra Clemo
Very pleased with cover, good quality and very smart
Date Added: 02/05/2018 by amanda derrick
I have bought a few of le mieux items inplum for my granddaughter. I am impressed with the quality of the goods and feel that I have received value...
Date Added: 05/01/2018 by Karen Bury


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Two bodies found
LODHRAN: Bodies of two men were found in a room near Dunyapur.
According to a police spokesman, bodies of two unidentified men were found on Saturday in a room in the Kotla Hassan Khan area of Dunyapur.
District Police Officer Malik Jameel Zafar, SP Investigations Syed Muhammad Abbas reached on the spot along with forensic team.
According to police, both the bodies were about a week old. One of the deceased was later identified as Ayub Bhutta, a drug addict, while the other body was still unidentifiable. Police are unsure of the cause of death.
Published in Dawn, December 9th, 2019


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Well done to this week's Mentoring learners for attending the Introduction to Mentoring Skills workshop here at Topps Training, funded by The National Lottery Community Fund.
Thank you for your lovely comments and feedback:
"I loved the knowledge I have gained from this course"
"The course was delivered in a relaxed but professional manor – great atmosphere"
"I loved the feedback we gained throughout, the mix of learning, group work and having the opportunity to work outside"
"It gave me the opportunity to meet some like minded people"
"The key thing I learnt by doing this course was the requirements expected of a mentor"
"Key thing I learnt on this course was the importance of safeguarding"
"Really good communication before the course"
"I loved the range of methods used to teach the group – lovely setting"
"This course will help me within my future role"
"This course encouraged me to speak to new people and to be more confident"
"The key thing I learnt was Mentoring is used every day in different ways"
Want to explore Mentoring? Learn new skills? Develop your confidence? then contact the Topps team today on 01902 652 307 to find our more about our workshops.


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Traveling north to our Northern Appalachian garden
- I've largely been posting on Places of the Spirit, if you're interested. This was my latest post, about visiting Ellis Island in NYC, with a loop around the Statue of Liberty.
Observations on nature and gardening
|view out the front door
|Heuchera reflecting sunset light
|pollinator garden
Labels: front door views, gardening for pollinators, pocket meadow


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Find and book unique accommodation on Airbnb
Guests agree: these stays are highly rated for location, cleanliness, and more.
Great apartment for providence as a family and business holiday. It is located in the very center of Rishon Letsion, in the best place with a lot of entertainment: excellent restaurants, bars, supermarkets, trade center ... Size 45 sq.m. with fully equipped kitchen and bathroom. The apartment has a large bed and another sofa that can be opened and transformed into a bed for 2 more people. The beach is 7 km away. Tel Aviv is located in 12 km away.
"I want to start with the most important thing! These are the owners of Roma and Natalia. We were greeted as relatives. In the apartment we were waiting for fruit and a bottle of wine. But this is the smallest thing that awaited us. In the three weeks spent at Roma and Natasha, we learned a lot about the culture of Israel, saw Tel Aviv and Jerusalem. We knew all the goodies of Friday dinner on the eve of Shabbat. And all thanks to them! In general, there are no words to describe all the hospitality that we experienced during our stay. Now about housing. The apartment is comfortable and clean. The four of us stayed. Me, wife and two daughters. We had enough space. The apartment has absolutely everything for a long stay. Even there is a Smart TV and very fast WiFi. Each room has air conditioning. Location of the apartment in the city center. Very convenient location for walking and shopping. There are cafes and bakeries with delicious bread at every step. 5 minutes walk there is a pleasant park for children, a Russian store, supermarkets, shopping centers, pharmacies and McDonald's. A 10-15 minute walk is the central bus station with direct buses to Jerusalem and Tel Aviv. From the airport 25 minutes without traffic jams."
Modern apartment with separate entrance in a private villa in prestigious district of Rishon Lezion. Completely equipped at the highest level. Sea beach in 15 min by car, Tel Aviv - in 15 min. Restaurants area, 10-15 min walk and 5 by car. Direct access to the highways. 20 min to the Ben Gurion airport, 40 min to Jerusalem. Taxis can be obtained via the phone application GETT or UBER with the arrival within 1-3 min to any point in Israel. Free parking is available 30 meters away. SmartTV+NETFLIX
"מיכאל היה מאוד נחמד ואדיב. כשביקשנו דברים שונים הוא ענה מיד ונתן מענה. התמונות באתר משקפות בדיוק את איך שהדירה נראית. יש מעט ברחשים אך אני מניח שדבר זה ייפתר כשיעשה ריסוס לקיץ. בנוסף, יש לו כלבה חמודה וחתולים. לפעמים הכלבה משוחררת בחוץ ואירחה לנו חברה כשישבנו למעלה. היא נובחת לפעמים במהלך היום וזה מעט הפריע כשדיברנו בטלפון לצורך שיחות עבודה, אך לדקה או שתיים. יש רווח קטן בדלת למטה ויכול להיות שמשם נכנסו קצת נמלות. בכל אופן, האירוח היה מצוין והדברים הפחות טובים מינוריים."
Apartment suitable for couples and families, spacious and comfortable apartment very pleasant. The apartment is in the best place in a prestigious area that has lots of green gardens and lots of shopping centers, cafes and restaurants in Rishon Lezion. 15 minutes from the city of Tel Aviv. The apartment is a 5-minute drive from the beautiful seaside promenade of Rishon LeZion. The apartment is a 30 minute drive From Jerusalem.
"Excellent apartment in the suburbs of Tel Aviv (on the South Side, so suited us as we have family in the area and in the South of Israel) Korin and Moshe are superb hosts. They waited for us in the apartment when we arrived. Explained all about the apartment and the neighbourhood. The apartment was sparkling clean and there were some basic food items to begin with. Plenty of shower gel, shampoo and conditioners, no need to carry from home. For the ladies - there was also a powerful. hairdryer. The apartment is within driving distance to a beautiful beach, free of charge, just need to pay parking. If you are religious, there are plenty of synagogues of all affiliations within walking distance. Building is quite new and has a parking spot and an elevator. It has 2 bed rooms and plenty of beds and sofas to be able to fit comfortably 6 guests of the same family. Small, but well equipped kitchen with ability to cook meals (we mostly ate with family and outside) with lots of shopping malls around to buy groceries and prepared meals. Overall - great stay and we will be back again for sure!"


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Did you know that there are actually 40 different species of seahorse? Which one is shown on this collection of bathroom decals, I hear you ask. Well, it doesn´t really matter - Because each seahorse is as magnificent as the next, so whether your favourite species is the dwarf, the longsnout, or even the cape - This collection of fish stickers is perfect for you! Stickers arrive on a sheet so they can be applied to your own liking.
Available in a variety of sizes. Like all of our decals, it is easy to apply and extremely long-lasting. Made using bubble-proof vinyl and leaves no damage or residue upon removal.
Wall color
Information about Seahorses Wall Stickers
Reference: A13325
Get your custom stickers with your images or personal text using the exclusive services offered through our website.


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The Editor: Can you answer some questions. LL ?
Answer Cat: I sure can.
CNN readers want to know why some of your information is a couple of days old ?
AC: Too many stories are taken from the " social media " smart phones and other electronic devices. They are often one-sided and show a limited view of the subject. The MSM takes the video of a few seconds of action as fact. They don't verify the accuracy. Here is a good example. I hope this kid wins.
Sometimes it takes time to sort through the basic facts. Here is another example.
A bunch of public figures make comments on Facebook, Twitter, etc. and later the truth comes out. People like Nancy Pelosi take down their comment with no explanation, after the fake firestorm has passed.
Who do you trust, AC ?
Almost no one in the news business, politics, late night comedians, or Hollywood.
Reflections on vdB 9
Image Credit & Copyright: Guenter Kerschhuber
Explanation: Centered in a well-composed celestial still life, pretty, blue vdB 9 is the 9th object in Sidney van den Bergh's 1966 catalog of reflection nebulae. It shares this telescopic field of view, about twice the size of a full moon on the sky, with stars and dark, obscuring dust clouds in the northerly constellation Cassiopeia. Cosmic dust is preferentially reflecting blue starlight from embedded, hot star SU Cassiopeiae, giving vdB 9 the characteristic bluish tint associated with a classical reflection nebula. SU Cas is a Cepheid variable star, though even at its brightest it is just too faint to be seen with the unaided eye. Still Cepheids play an important role in determining distances in our galaxy and beyond. At the star's well-known distance of 1,540 light-years, this cosmic canvas would be about 24 light-years across.
Tomorrow's picture: pixels in space


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Flexa Cabby Cupboard 2 Doors
Regular Price: £302.00
Special Price £256.70
Need help? Get in touch or chat now
Found it cheaper? Then contact us about our price match
- Description
Product Description:
FLEXA Cabby cupboard can be combined, placed sideways or on top of other Cabby building blocks.
It allows you to create a customised storage solution to best fit the needs of the child.
All Cabby system building blocks are made of high quality MDF, has the same depth and width to make storage combination process even easier.
Cupboard with 2 doors incl. adjustable feet.
Dimensions: Height: 51.2cm x Width: 101cm x Depth: 56.5cm
- Images
- Reviews


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Nelli 22 y.o. (Kazakhstan)
Name: Nelli
Profile number: w634
Birth date: 28 June
Age: 22
Height: 163 cm 5' 4"
Weight: 52 kg 114.7 lbs
Country: Kazakhstan
City: Almaty
Education: University
Profession: Student
Marital Status: Single
Languages: Upper intermediate English, Russian, Kazakh
Hobbies: Cinema, meeting my friends, reading books, listening to classical music. From time to time I like making experiments in the kitchen.
Self Description: I am loyal, honest and very friendly girl but with a strong character and good sense of humor. I am also very sociable and I love people as much as I love myself.
Seeks Partner: 25-32 y.o. Educated, decent, honest, ready financially and morally for serious relationship and marriage.
E-mail: Yes
Phone: -
Postal address: Yes
To get the contact address of this lady and to correspond with her directly:
- For Members, please Click Here
- If you are not a member yet, Please go to Become a Member section.


text:
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- Secure Storage Containers in Fremont, NE.
- All Steel Container Products.
- Rent or Purchase.
Buy & Rent Steel Storage Containers in Fremont
When you need storage containers in Fremont you can count on Cork'd to get the best prices, selection and customization you need. Whether you are looking to rent or need a used storage container for sale in Fremont you don't need to go anywhere else. Cork'd helps you quickly research Fremont storage container pricing faster than ever before. Whether you have some excess inventory during a busy season, or you want to keep equipment and tools safe and secure on site, buying or renting a steel storage container in Fremont can be the perfect solution to your problem.
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Fremont Steel Storage Containers Offered:
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Give us a call or request a quote to start comparing prices from the best Fremont storage container dealers serving your area. You won't find more secure conex storage containers or lower prices anywhere else!
Non-Verified Storage Containers Companies
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2611 Kimco Dr
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Storage Containers USA Lincoln
319 B St
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Omaha Storage Container HQ
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Storage Containers USA Omaha
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Total Tool Supply
4515 S 90th St
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40 Ft Container Rental
$ 189/unit per month
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$ 189/unit per month
What is minimum monthly payment on a forty ft storage container for about four months?
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Construction site needs a container for 2 months for the whole of the building project.
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What does it cost for a few 30 ft. storage containers dropped off in Fremont?
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Job site needs a container for less than a month all through building.
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$ 90/unit per month
How much is a twenty ft. storage container for 3 months?
Courtney S.
Mead, NE, 68041
20 Ft Container Rental
$ 3505/unit


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A show of confidence or a sign of desperation? For now, it seems to be both. In order to counteract the severe impact that the Galaxy Note 7 had on its image, Samsung is believed to be pulling all the stops in marketing the Galaxy S8. Apparently, that includes offering a 3-month refund policy, where dissatisfied owners can return the (undamaged) device, no questions asked. The unorthodox policy is supposedly a way for Samsung to give its Bixby assistant time to mature.
Samsung has already confirmed that Bixby is not only real but will also make its way to its next flagship, the Galaxy S8. But the Bixby that will debut next week is not the Bixby that has been speculated for the past months. Specifically, the personal assistant will not yet incorporate technology and software from Samsung's newly acquired Viv Labs.
It will eventually be smarter, but not immediately at launch. Samsung apparently thinks that it can roll out a major update that will significantly improve Bixby within three months of the Galaxy S8's launch. Hence, owners who are less than satisfied with their Bixby experience can return the device within that time.
The refund policy, however, also touches on the OEM's attempts to bring back confidence in its brand and most especially its smartphones. The rather wide refund window could be interpreted as Samsung showing confidence that, this time around, the Galaxy S8 won't be a fire hazard. Reports of the Galaxy Note 7's explosive tendency started pouring in within three months of its launch.
The refund policy, if true, is definitely a bold move for Samsung, though it probably won't be as straightforward as Samsung might make it out to be. "No questions asked" is probably just a dream, and there will most likely be hoops to go through to get a refund.
VIA: Chosun Ilbo


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Trailing twelve-month data shows us that CYCLIQ Group Limited's (ASX:CYQ) earnings loss has accumulated to -AU$4.31M. Although some investors expected this, their belief in the path to profitability for CYCLIQ Group may be wavering. Savvy investors should always reassess the situation of loss-making companies frequently, and keep informed about whether or not these businesses are in a strong cash position. This is because new equity from additional capital raising can thin out the value of current shareholders' stake in the company. Given that CYCLIQ Group is spending more money than it earns, it will need to fund its expenses via external sources of capital. Looking at CYCLIQ Group's latest financial data, I will gauge when the company may run out of cash and need to raise more money. Check out our latest analysis for CYCLIQ Group
What is cash burn?
CYCLIQ Group's expenses are currently higher than the money it makes from its day-to-day operations, which means it is funding its overhead with equity capital a.k.a. its cash. With a negative operating cash flow of -AU$3.38M, CYCLIQ Group is chipping away at its AU$910.11K cash reserves in order to run its business. The measure of how fast CYCLIQ Group goes through its cash reserves over time is called the cash burn rate. Companies with high cash burn rates can eventually turn into ashes, which makes it the biggest risk an investor in loss-making companies face. CYCLIQ Group operates in the leisure products industry, which on average generates a positive earnings per share, meaning the majority of its peers are profitable. CYCLIQ Group faces the trade-off between running the risk of depleting its cash reserves too fast, or risk falling behind its profitable competitors by investing too slowly.
When will CYCLIQ Group need to raise more cash?
Operational expenses, or opex for short, are the bare minimum expenses for CYCLIQ Group to continue its operations. In this case I've only accounted for sales, general and admin (SG&A) expenses, and basic R&D expenses incurred within this year. Opex (excluding one-offs) grew by 25.00% over the past year, which is considerably high. My cash burn analysis suggests that, if CYCLIQ Group continues to spend its cash reserves at this current high rate, it'll have to raise capital within the next 3 months, which may be a surprise to some shareholders. Moreover, even if CYCLIQ Group kept its opex level at AU$4.51M, it will still have to come to market within the next year. Although this is a relatively simplistic calculation, and CYCLIQ Group may reduce its costs or open a new line of credit instead of issuing new equity shares, the analysis still helps us understand how sustainable the CYCLIQ Group's operation is, and when things may have to change.
Next Steps:The risks involved in investing in loss-making CYCLIQ Group means you should think twice before diving into the stock. However, this should not prevent you from further researching it as an investment potential. The cash burn analysis result indicates a cash constraint for the company, due to its high opex growth and its level of cash reserves. This suggests an opportunity to enter into the stock, potentially at an attractive price, should CYCLIQ Group come to market to fund its growth. I admit this is a fairly basic analysis for CYQ's financial health. Other important fundamentals need to be considered alongside. You should continue to research CYCLIQ Group to get a better picture of the company by looking at:
- Historical Performance: What has CYQ's returns been like over the past? Go into more detail in the past track record analysis and take a look at the free visual representations of our analysis for more clarity.
- Management Team: An experienced management team on the helm increases our confidence in the business – take a look at who sits on CYCLIQ Group's board and the CEO's back ground.
- Other High-Performing Stocks: If you believe you should cushion your portfolio with something less risky, scroll through our free list of these great stocks here.


text: ngale65 0 Report post Posted December 12, 2011 http://www.ebay.com/itm/Allis-Chalmers-586-Sno-Bee-Snowblower-1968-/260914928608?pt=LH_DefaultDomain_0&hash=item3cbfbddbe0#ht_500wt_1363 was looking at ebay and saw this. thats just awesome. i wish it were in indiana. hope someone gets it in the club. thought everyone would at least like the pictures nathan Share this post Link to post Share on other sites


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Developing Services for Older Women Who Are Victims of Domestic Violence or Sexual Assault
Author: Lori Stiegel, J.D..; Candace Heisler, J.D..; Bonnie Brandl, M.S.W..; Amy Judy, J.D..
Source: Volume 02, Number 02, Fall 2009 , pp.183-191(9)
next article > |return to table of contents
Abstract:Older women who are victims of domestic violence and sexual assault often need specialized services. This article provides an overview of the challenges of working with this population of victims and the types of services they need. While older and younger victims of domestic violence share many common needs, older women often face additional challenges (e.g., issues related to collecting Social Security). Further, shelters are frequently not set up to deal with the needs of battered elderly women. The author offers suggestions for enhancing the response to the needs of older victims, including developing rapid response teams, advocates who specialize in elderly victims, and cross training of domestic violence/aging services providers.
Affiliations: 1: American Bar Association Commission on Law and Aging; 2: University of California at Hastings; 3: Wisconsin Coalition Against Domestic Violence; 4: Disability Rights Wisconsin.


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NAMI Connection Support Group in Ellsworth
- This event has passed.
December 2, 2019 @ 12:00 pm - 1:30 pm
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NAMI Connection peer support group is planned for Mon Dec 2 from noon to 1:30 pm. This is a free and confidential support group for adults who have mental health challenges or mental illness. Light refreshments are provided. Meetings take place in the Moore Community Center Conference Room, 133 State St., Ellsworth, every first Monday of the month. For more information, call Betsy Rose, 866-3307.


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This was my beautiful sister's Baby Shower and of course, I was very excited to do it! Especially at this amazing rooftop venue located at the Star Tower Condominiums in Downtown Orlando. … [Read more...]
Woodland Christmas Rum Cake Bar
Are you one of those people who buy rum cakes as gifts every time you go on a tropical vacation? I used to be, until my husband perfected his recipe. Now, I just ask him to make … [Read more...]
A Mother's Day Brunch for the special moms in my life
Happy Mother's Day to all mamas out there, including pet mamas and mamas to be!!! Mother's Day is always so special because I have all the moms in my family close to me! Each year … [Read more...]
Be my Valen-corn Party
Remember when unicorn cakes were everything? I'm glad I had the opportunity to do one for this super cute Valentine's Day Party before we send them back to the magical forest where they came from! … [Read more...]


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His incoherent ramblings
I have to say a big thanks to all at the .NET Developers Network in Bristol for inviting me down to talk about Virtualisation for Developers. Chris and Guy were great hosts, and it was an impressive turnout considering many had already attended the 'extra' event on the Monday when Oren Eini came to visit.
Chatting with the audience at the half time break and after the presentation raised areas I hadn't researched. I only hope I can answer some of the questions that were posed. This subject seems to get bigger and bigger – stripping it down to 60 minutes for DDD7 is going to be a challenge.
It was also great to head down to the Student Union bar for a couple of pints of Doom Bar. The only shame was leaving early to catch the train from Bristol Parkway (thanks to Guy for the lift in the car) to discover my train was 40 minutes late. Curse you! First Great Western - that was beer drinking time you stole from me.
Thanks again to Guy and Chris.
Print | posted on Thursday, October 16, 2008 11:20 PM
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Peaceful, beautiful, WET!
I visited this campground in October of 2018. The campground itself is beautiful, but recently part of it had been submerged in water which was problematic for us tent campers. Sunset Point is beautiful, but I'd recommend it only in the summer and on weekends when there is no chance of rain. We were soaked all weekend. I'd definitely return because the park has nice trails, great views, and a serene lake setting, but I'd make sure I'm in a different campground than Sunset Point.


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SageBrush wrote: ↑Tue Jul 23, 2019 4:22 pmFair enough, thanks for tracking this down.danrjones wrote:I actually looked and according to the warranty, under "what is NOT covered" includes exposing the car to "ambient temperatures above 120F (49C) for 24 hours"
Owners better hope that the sensor is not near the battery.
it's not clear if the 24 hours is consecutive or cumulative?
????Is the 24 hours consecutive????
???? Is the 24 hours total during the warranty period???
???? Would 12 hours at 120F then 12 hours at 110F then 12 at 120F again be considered 12 hours for warranty issue????


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Support a Model, click the image below
The heart and soul of the event are the fundraising ambassadors who agree to raise a minimum of $1,500 by participating in a friendly fundraising competition.
Why Support Pooches on Parade Fashion for Paws benefiting the Fuzzy Friends Rescue Angel Heart Medical Fund? • The Angel Heart Medical Fund helps to provide "intensive" medical care for injured or severely neglected animals needing life-saving medical treatment, surgical procedures, and rehabilitation. • Fuzzy Friends Rescue treats more than 50 dogs per year for heartworms. • Fuzzy Friends Rescue saves life – more than 17,000 animals have been saved since becoming incorporated as a non-profit in 1997.
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Rheb, a Ras-like small GTPase conserved from human to yeast, controls Tor kinase and plays a central role in the regulation of cell growth depending on extracellular conditions. Rhb1 (a fission yeast homolog of Rheb) regulates amino acid uptake as well as response to nitrogen starvation. In this study, we generated two mutants, rhb1-DA4 and rhb1-DA8, and characterized them genetically. The V17A mutation within the G1 box defined for the Ras-like GTPases was responsible for rhb1-DA4 and Q52R I76F within the switch II domain for rhb1-DA8. In fission yeast, two events—the induction of the meiosis-initiating gene mei2+ and cell division without cell growth—are a typical response to nitrogen starvation. Under nitrogen-rich conditions, Rheb stimulates Tor kinase, which, in turn, suppresses the response to nitrogen starvation. While amino acid uptake was prevented by both rhb1-DA4 and rhb1-DA8 in a dominant fashion, the response to nitrogen starvation was prevented only by rhb1-DA4. rhb1-DA8 thereby allowed genetic dissection of the Rheb-dependent signaling cascade. We postulate that the signaling cascade may branch below Rhb1 or Tor2 and regulate the amino acid uptake and response to nitrogen starvation independently.
SIGNALING involving mTOR kinase is evolutionally conserved from yeast to human. In vertebrates, it regulates cell growth depending on the availability of nutrients, energy source, and growth factors (Hay and Sonenberg 2004; Wullschleger et al. 2006; Guertin and Sabatini 2007). The mTOR signaling involves a Ras-like GTPase, Rheb that promotes protein synthesis and thereby cell growth via mTOR (Saucedo et al. 2003; Stocker et al. 2003). When the environment surrounding the cell is not favorable for growth and proliferation, the protein complex of TSC1 and TSC2, which has a GAP GTPase-activating protein (GAP) activity, converts Rheb into a GDP-bound form (Inoki et al. 2003; Tee et al. 2003; Zhang et al. 2003; Li et al. 2004b). A loss of TSC1/2 would thereby result in constitutive upregulation of the Rheb GTPase and of mTOR and, in humans, allow the wide spread of benign tumors termed "hamartomas" in different organs including the brain, eyes, heart, kidney, skin, and lungs (Manning and Cantley 2003; Li et al. 2004a; Pan et al. 2004).
In fission yeast, a prototype of the TOR-signaling system consists of Tsc1/2, Rhb1 GTPase (a homolog of Rheb GTPase), and Tor2 kinase (a homolog of mTOR), as in the mTOR-signaling system in higher eukaryotes (Otsubo and Yamamato 2008). It has been demonstrated by a number of studies that the Tor signaling in fission yeast controls various biological responses to starvation of nitrogen. Upon starvation of nitrogen, fission yeast cells are arrested at G1 with small- and round-cell morphology following two rounds of rapid cell cycling without net growth (Young and Fantes 1987). They also induce expression of the mei2+ gene that is required for initiation of meiosis (Watanabe et al. 1988). Upon a loss of function of tor2+ gene, these events are induced even under a nitrogen-rich condition, indicating that Tor2 is responsible for suppression of the events that are normally induced upon nitrogen starvation (Alvarez and Moreno 2006; Uritani et al. 2006; Hayashi et al. 2007; Weisman et al. 2007; Matsuo et al. 2007). The events adaptive to nitrogen starvation are important for the preservation and survival of the organism, but they are deleterious for actively growing cells. Thus, it is critical for the cell to strictly control the activity of the Tor2 kinase depending on the nutrient condition.
Rheb/Rhb1 GTPase plays a central role as a signal carrier in the Tor signaling. In Drosophila, it has been shown that Rheb promotes cell growth via the Tor-signaling system (Saucedo et al. 2003; Stocker et al. 2003). In fission yeast, a loss of rhb1+ causes a cell cycle arrest at G1 and induction of fnx1+ and mei2+ genes, both of which are normally suppressed by Tor2 (Mach et al. 2000; Matsuo et al. 2007). These genetic studies have indicated that Rheb/Rhb1 positively regulates Tor kinase. Biochemical studies have also demonstrated close interaction between Rheb/Rhb1 GTPase and Tor kinase. It has been shown that when Rheb is ectopically expressed, it binds to mTOR (Long et al. 2005). Subsequently, it has been demonstrated that purified Rheb loaded with GTP, but not with GDP, activates mTOR kinase in vitro (Sancak et al. 2007). Ras-like small GTPases, in general, bind to and stimulate their targets only when they are loaded with GTP. The above two studies, however, have reported that binding between Rheb and mTOR is not influenced by the guanine-nucleotide binding state of Rheb. In contrast, Rhb1 in a GTP-bound form binds to Tor2 kinase in fission yeast (Urano et al. 2005; Uritani et al. 2006). Another study has demonstrated that GTP-bound Rheb directly binds to FKBP38, a member of the FK506-binding protein, and protects mTOR from inhibition by FKBP38 (Bai et al. 2007), suggesting that Rheb activates mTOR rather indirectly, although this model has been disputed by a recent study (Wang et al. 2008). Obviously, further investigation is needed to formulate a general view of a molecular mechanism by which Rheb/Rhb1 GTPase interacts with Tor as well as with other components.
In our previous studies, we showed that deletion of the tsc2+ gene encoding a GAP for Rhb1 GTPase confers a defect in uptake of amino acids (Matsumoto et al. 2002) and prevents induction of the mei2+ gene upon nitrogen starvation (Nakase et al. 2006). Rhb1 GTPase, which is assumed to be in a GTP-bound form in cells lacking Tsc2 (Δtsc2), is likely a cause of these defects. In this study, we generated two dominant active rhb1 mutants and found that they distinctively respond to nitrogen starvation. One mutant (rhb1-DA4) exhibits the phenotypes identical to that of Δtsc2. The other one (rhb1-DA8), however, is defective in uptake of amino acids, but allows induction of the mei2+ gene upon nitrogen starvation. Remarkably, rhb1-DA8 also allows induction of the mei2+ gene in the Δtsc2 background. On the basis of these results, we propose a model for Rhb1 signaling in fission yeast.
Yeast strains, media, and transformation:
The Schizosaccharomyces pombe strains used in this study are listed in Table 1. The yeast cells were grown in yeast extract with supplements (YES) media and EMM synthetic minimal media with appropriate nutrient supplements as described by Moreno et al. (1991). All yeast transformations were carried out by lithium acetate methods (Okazaki et al. 1990; Gietz et al. 1992).
Mutagenesis of rhb1+:
The rhb1+ gene was amplified by PCR using the forward primer 5′-rhb1 [5′-GGGGGGGGGTCGAC(SalI)ATGGCTCCTATTAAATCTCGTAGA-3′] and the reverse primer 3′-rhb1 [5′-CCCCCCCCGGATCC(BamHI)TTAGGCGATAACACAACCCTTTCC-3′]. To introduce mutations, PCR was performed in the presence of 0.5 mm Mn2+ and 1 mm Mg2+. The resulting fragments were digested with BamHI and SalI and then cloned into pREP81 to construct a pool of rhb1 mutants.
Deletion of rhb1+ gene:
A plasmid to delete the rhb1+ gene, pBS-Δrhb1, was constructed by PCR amplification of a 1.1-kbp DNA fragment upstream of the start codon of the rhb1+ gene and a 1.4-kbp DNA fragment downstream of the stop codon. The primer sequences used in the PCR were as follows: for amplification of the upstream sequence, the forward primer was rhb1-NotI-1 [5′-CCCCCCGCGGCCGC(NotI)GTGTAAAGGGAGCCGTTCAAG-3′] and the reverse primer was rhb1-BamHI-2[5′-CCCCCCGGATCC(BamHI)GGCAAATTATTAACTATAGAG-3′]; for the downstream sequence, the forward primer was rhb1-BamHI-3 [5′-CCCCCCGGATCC(BamHI)GCTTCTGCTTGAATTTATC-3′] and the reverse primer was rhb1-SalI-4 [5′-CCCCCCGTCGAC(SalI)GTACGTTCAATTCCTATTC-3′]. The resulting DNA fragments were digested with combinations of appropriate restriction enzymes and then ligated into pBS plasmids to create pBS-rhb1-up and pBS-rhb1-down. The pBS-rhb1-down plasmid was digested with NotI and BamHI and ligated with a NotI-BamHI fragment that contained the rhb1 upstream sequence isolated from pBS-rhb1-up to create pBS-rhb1-up-down. pBS-rhb1-up-down was digested with BamHI and ligated with a DNA fragment that contained the ura4+ gene to create pBS-Δrhb1. Digestion of pBS-Δrhb1 with NotI and SalI generated a 4.3-kbp DNA fragment containing the ura4+ gene flanked by the 1.1-kbp upstream and the 1.4-kbp downstream sequences of the rhb1+ gene, which was used for transformation to delete the rhb1+ gene in a diploid strain.
Spot test:
Cells were cultured in liquid YES or EMM medium at a concentration of 1 × 107 cells/ml, and each culture was diluted by a factor of 10. Five microliters of each suspension was spotted onto appropriate media.
Western blotting:
Total cell lysates were prepared as follows. Cells were lysed with glass beads in 1× PBS (140 mm NaCl, 2.7 mm KCl, 1.5 mm KH2PO4, and 8.1 mm Na2HPO4) containing 1 mm MgCl2, 0.5% Triton X-100, and 0.5% deoxycholate. The following protease inhibitors were added to the cell extracts: 1 mm phenylmethylsulfonyl fluoride and 1× protease inhibitor cocktail (Nacalai Tesque). Equal amounts of total proteins were then loaded onto a 15% polyacrylamide gel and transferred to nitrocellulose membranes. Filters were probed with anti-Rhb1 rabbit polyclonal antibodies (Nakase et al. 2006) at a 1:2000 dilution. Blots were also probed with an anti-α-tubulin monoclonal antibody, TAT-1 (a gift from K. Gull, University of Manchester, Manchester, UK), to normalize protein loading. Other procedures used were Northern blotting and microarray analysis, which were performed as described previously (Nakase et al. 2006; Chikashige et al. 2007).
Screen for rhb1 mutants:
We and other groups previously showed that a loss of Tsc2 (GTPase-activating protein for Rhb1 GTPase) causes a reduction in the uptake of amino acids and resistance to canavanine, a toxic analog of arginine (van Slegtenhorst et al. 2004; Nakase et al. 2006). Rheb GTPase, which would predominantly remain as a GTP-bound form in the cells lacking the Tsc2 activity, is likely a cause of the low uptake of amino acids and the resistance to canavanine. We reasoned that a mutation on the rhb1+ gene, which can stably maintain bound GTP or abnormally activate a downstream component, might confer a phenotype similar to that observed in cells lacking Tsc2 (Δtsc2). To obtain such a mutation, a pool of the rhb1 mutants was generated by error-prone PCR. Individual PCR products were cloned into a plasmid, pREP81, which allowed expression of a cloned gene from an inducible promoter, nmt1, and transformed into a wild-type strain. We screened for transformants, which became resistant to canavanine upon expression of the cloned rhb1 mutant. Among ∼40,000 transformants, we found 3 exhibiting strong resistance to canavanine upon expression of the rhb1 gene, which presumably carried a mutation.
Construction of rhb1-DA4 and rhb1-DA8:
The plasmids were recovered from the three transformants, and the nucleotide sequence of the rhb1+ gene (designated rhb1-DA4, -DA5, and -DA8, respectively) was analyzed. Because we found that the rhb1 gene isolated from each transformant carried multiple mutations, we determined the mutation sites responsible for conferring the resistance to canavanine. As shown in Figure 1A, the mutation of rhb1-DA4 responsible for conferring the resistance to canavanine is valine at position 17 replaced with alanine (V17A). The rhb1-DA5 carries the responsible mutation at the same position, but replaced with aspartic acid (V17D). The rhb1-DA8 carries two mutations replacing glutamine at position 52 with arginine (Q52R) and isoleucine at position 76 with phenylalanine (I76F), both of which are necessary for conferring the resistance to canavanine (Figure 1A).
A previous report (Urano et al. 2005) described several hyperactive mutations of the fission yeast rhb1 gene, including V17A (identical to rhb1-DA4) and V17G within the G1 box, a domain important for guanine nucleotide binding (Figure 1B and see discussion). Biochemical analysis indicated that the V17G mutation inhibits both GTP and GDP binding. It is thereby plausible that Rhb1 GTPase stimulates its effector regardless of the guanine-nucleotide-binding status if valine at position 17 is mutated to glycine or alanine. The rhb1-DA8 contains mutations at Q52 and I76, both of which are not conserved in other small GTPases and thus its biochemical character is less predictable.
We introduced the rhb1-DA4 and rhb1-DA8 to the native rhb1 locus. In the heterozygous diploid strain rhb1+/Δrhb1, the deletion allele (Δrhb1) was replaced with either the rhb1-DA4 allele or the rhb1-DA8 allele. The resulting heterozygous diploids were sporulated, and each of them was found to successfully produce four viable spores. We determined the genotype of each cell by sequencing the rhb1 locus and confirmed that each diploid produced two wild-type cells and two rhb1 mutants.
Genetic analysis of rhb1-DA4 and rhb1-DA8:
We examined the rhb1-DA4 and rhb1-DA8 mutants for their resistance to canavanine. As shown in Figure 2A, the two mutants were resistant to canavanine, although the rhb1-DA4 mutant was resistant to a lesser extent. We also found that these mutants were defective in uptake of leucine (Figure 2B). The heterozygous diploids rhb1+/rhb1-DA4 and rhb1+/rhb1-DA8 were also resistant to canavanine (Figure 2C), indicating that these two mutations are dominant.
We previously showed that a loss of the cpp1+ gene (cpp1-1) suppresses defects associated with Δtsc2 (Nakase et al. 2006). The cpp1+ gene encodes an enzyme that farnesylates the C terminus of Rhb1 GTPase. We speculate that a failure in farnesylation results in a partial loss of the Rhb1 function, which contributes to the suppression of Δtsc2. To test whether the cpp1-1 mutation could suppress rhb1-DA4 and rhb1-DA8, it was introduced into each of the rhb1 mutants. As shown in Figure 2D, the cpp1-1 mutation fully suppressed the resistance to canavanine in rhb1-DA4. In contrast, the suppression of the resistance by the cpp1-1 mutation was only partial in the rhb1-DA8 mutant. We also examined Rhb1-DA4 and Rhb1-DA8 for their modification by Cpp1. It was shown previously that Rhb1 GTPase in fission yeast (Yang et al. 2000) migrates faster on SDS–PAGE if properly modified. As shown in Figure 2E, Rhb1, Rhb1-DA4, and Rhb1-DA8 were detected as a doublet in cell extracts prepared from the cpp1-1 mutant grown at 26°. Three hours after the shift to the restrictive temperature 36°, the slower-migrating form of Rhb1 increased. In cell extracts prepared from the wild-type background, only faster-migrating forms of Rhb1-DA4 and Rhb1-DA8 were detected. These results indicated that Rhb1-DA8 was not fully modified with a farnesyl group in the cpp1-1 mutant even at 26°. It is thus likely that Rhb1-DA8 GTPase is less dependent on the modification by Cpp1 to cause the resistance to canavanine.
Response to nitrogen starvation:
Fission yeast cells, when grown in nitrogen-free media, undergo stimulated rates of division mostly due to a shortened G2 phase, a period important for cell growth for this organism (Young and Fantes 1987). As a result, cells become small and round. They also allow induction of the meiosis-initiating gene mei2+. These two events are a hallmark of the response to nitrogen starvation in fission yeast. In this study, the two dominant active rhb1 mutants, along with Δtsc2, were examined for their response to nitrogen starvation in detail.
We first examined cell morphology and rates of cell division after nitrogen starvation. As shown in Figure 3, 4 hr after the shift to the nitrogen-free media, the wild-type cells became round and small. Its growth rate was stimulated upon nitrogen starvation (Figure 4 ). In contrast, Δtsc2 and the rhb1-DA4 mutant responded minimally to nitrogen starvation. Their morphology did not change dramatically (Figure 3), and their rates of division were lower in the absence of a nitrogen source (Figure 4). We also examined the rhb1-DA8 mutant and unexpectedly found that it behaved just like the wild-type strain in the nitrogen-free media. As shown in Figures 3 and 4, it exhibited shorter and round morphology after stimulated cell division upon the shift to the nitrogen-free media.
We next examined expression of three genes, which are abnormally expressed in Δtsc2 upon the shift to the nitrogen-free media. The mei2+ gene that is induced in the wild-type strain is not induced in Δtsc2. On the other hand, the inv1+ gene and Tf2 (a transposable element) that are not induced in the wild-type strain are induced in Δtsc2 (Nakase et al. 2006).
As shown in Figure 5A, in the rhb1-DA4 mutant, the mei2+ gene was not induced and the inv1+ gene and Tf2 were induced. In addition, we found that introduction of cpp1-1, a suppressor of Δtsc2, also suppressed the phenotype of rhb1-DA4 (Figure 5B). These results indicated that the rhb1-DA4 mutation causes phenotypes indistinguishable from that of Δtsc2. In contrast, expression of the three genes was normal in rhb1-DA8. Upon nitrogen starvation, the mei2+ gene was induced and the two genes, inv1+ and Tf2, were not (Figure 5A). We also analyzed the gene expression profile in a genomewide scale by microarrays. As shown in Table 2, while a number of genes were abnormally expressed in Δtsc2 and the rhb1-DA4 mutant, they were normally expressed in the rhb1-DA8 mutant.
Suppression of Δtsc2 by rhb1-DA8:
The results described above uncovered a very unique feature of the rhb1-DA8 mutation. Although it strongly confers the resistance to canavanine in a dominant manner, the rhb1-DA8 mutation does not affect the responses to nitrogen starvation that are induction of the mei2+ gene and stimulated cell division with no net growth. Because these two events are regulated by the Tor2 kinase (Alvarez and Moreno 2006; Uritani et al. 2006; Hayashi et al. 2007; Matsuo et al. 2007; Weisman et al. 2007), we speculated that (1) the rhb1-signaling cascade branches into two pathways, the Tor2-dependent pathway and an unknown one, and (2) the Rhb1-DA8 GTPase might preferentially stimulate the unknown target that is responsible for conferring the resistance to canavanine. To further understand the nature of the rhb1-DA8 mutation, we constructed a double mutant, Δtsc2 rhb1-DA8, and examined it for the response to nitrogen starvation.
As shown in Figure 4 and Figure 6A, upon the shift to the nitrogen-free media, the double mutant became shorter and round following a stimulated division. It also allowed induction of the mei2+ gene (Figure 6B). These results suggested that the Rhb1 GTPase, which would remain in a GTP-bound form in Δtsc2, cannot stimulate the Tor2 kinase in the rhb1-DA8 background.
Interaction between Tor and Rhb1-DA:
To further explore the functional relationship between Tor2 and Rhb1, we attempted to examine the effect of the rhb1-DA4 and rhb1-DA8 mutations on a temperature-sensitive allele of tor2, tor2-ts6, or tor2-ts10 (Matsuo et al. 2007). As shown in Figure 7A, the tor2-ts6 mutant was resistant to canavanine, but to a lesser extent compared with the rhb1-DA4 or Δtsc2 single mutants. We introduced rhb1-DA4, a mutation previously characterized as hyperactive (Urano et al. 2005), into tor2-ts6 and found that the double mutant exhibited a canavanine resistance similar to that of the rhb1-DA4 single mutant (Figure 7A). We also constructed the double mutant tor2-ts6 Δtsc2 and found that it exhibited a resistance to canavanine similar to that of the Δtsc2 mutant. These results thereby indicated that the resistance to the drug conferred by rhb1-DA4 or Δtsc2 was not influenced by tor2-ts6. The resistance to canavanine conferred by tor2-ts6 was likely due to a defect in uptake because the tor2-ts6 leu1-32 mutant, whose growth was dependent on leucine supplemented in synthetic media, grew poorly on EMM media containing leucine at a concentration of 40 μg/ml (Y. Nakase, unpublished result).
The crosses between the tor2-ts6 mutant and the rhb1-DA8 mutant produced tetrads in which only two or three spores were viable (Figure 7B). We determined the genotype of each viable spore by sequence analysis to identify the rhb1-DA8 mutation and by testing the temperature sensitivity to identify the tor2-ts6 mutation. We could thereby deduce the genotype of the nonviable spores. The results indicated that all the nonviable spores were double mutants (tor2-ts6 rhb1-DA8). Other tetrads produced four viable spores, two of which were tor2-ts6 and the other two of which were rhb1-DA8 (not shown). We thus concluded that the rhb1-DA8 mutation caused a synthetic lethality with the tor2-ts6 mutation. The crosses between the tor2-ts10 mutant and the rhb1-DA8 mutant produced double mutants (tor2-ts10 rhb1-DA8), which could occasionally form tiny colonies after incubation for >10 days. We, however, failed to isolate a double-mutant strain due to spontaneous reversion (not shown). These results indicated that the rhb1-DA8 allele produced a synthetic lethal or sick phenotype when combined with the tor2-ts alleles. Rhb1-DA8 could not normally stimulate Tor2.
Because we could not construct a double mutant (rhb1-DA8 tor2-ts6), the rhb1-DA8 mutation was introduced ectopically into the tor2-ts6 mutant by transformation. As shown in Figure 7C, upon introduction of the rhb1-DA8 gene into the tor2-ts6 mutant, the mutant became more resistant to canavanine. Similarly, the rhb1-DA4 gene conferred a stronger resistance to the drug when it was introduced ectopically into the tor2-ts6 mutant.
We finally examined the functional relationship between Tor1 and Rhb1. As shown in Figure 7D, the resistance to canavanine conferred by rhb1-DA4, rhb1-DA8, or Δtsc2 was not fully suppressed by Δtor1, indicating that Tor1 does not directly interact with Rhb1 to cause the resistance to the drug.
In this study, we generated two dominant active rhb1 mutants, rhb1-DA4 and rhb1-DA8, and characterized them genetically in fission yeast. Although the two mutants were isolated through the same genetic screen, they responded to nitrogen starvation quite differently.
Two dominant active mutants, rhb1-DA4 and rhb1-DA8:
In the rhb1-DA4 mutant, valine at position 17 is replaced with alanine. Ras and other small GTPases are built with several conserved functional domains, namely, G1, G2, G3, G4, and G5 boxes (Bourne et al. 1991). The V17A mutation is located in the G1 box (Figure 1B) that contacts phosphates of GTP or GDP. This mutation (V17A) and a similar one (V17G) in fission yeast Rhb1 GTPase were also identified previously in a screen similar to that reported in this study. It was demonstrated that the mutation of V17G abolishes the guanine nucleotide binding (Urano et al. 2005). Activating mutations in budding yeast Ras2 at an analogous position such as V21D and V21G were also shown to abolish the nucleotide binding (Dalley and Cannon 1996). Our analysis in this study indicated that the rhb1-DA4 mutation mimics Δtsc2, in which Rhb1 GTPase would remain as a GTP-bound form due to loss of the Tsc2-dependent GAP activity. Taken together, we speculate that rhb1-DA4 can stimulate its downstream elements regardless of the guanine-nucleotide-binding status.
The rhb1-DA8 carries two mutations replacing glutamine at position 52 with arginine (Q52R) and isoleucine at position 76 with phenylalanine (I76F). The amino acids at these positions are conserved in human Rheb (Figure 1B). Of the two mutations in rhb1-DA8, I76F is located within switch II, a domain considered to be important for interaction with GAP and effectors (Vetter and Wittinghofer 2001; Yu et al. 2005). A recent study of human Rheb has demonstrated that a double mutation of I76A D77A almost completely abolishes the ability to stimulate its effector, mTOR, although the mutation does not affect binding to mTOR (Long et al. 2007). These results would suggest that the I76F mutation in rhb1-DA8 might affect the ability of Rhb1 GTPase to stimulate the Tor2 kinase. While the rhb1-DA8 mutant exhibits strong resistance to canavanine, it allows response to nitrogen starvation. The mutant divides without net growth and allows induction of the mei2+ gene upon the shift to the nitrogen-free media. Because these events that are adaptive to nitrogen starvation are normally suppressed by the Tor2 kinase, the phenotype of the rhb1-DA8 mutant would also suggest a weak activity of Rhb1-DA8 GTPase to stimulate Tor2. More directly, the rhb1-DA8 mutation causes a synthetic lethality with the tor2-ts6 mutation (Figure 7B). On the basis of the results from our genetic analysis and the preceding biochemical and structural studies of human Rheb, it is likely that the Rhb1-DA8 GTPase is partially defective in activating the Tor2 kinase.
Roles of the Tor2 kinase in uptake:
It was previously shown that overexpression of tor2+ caused a resistance to canavanine in a wild-type strain, but not in a strain deleted for the tor1+ gene (Weisman et al. 2007). Activation of Tor2 kinase may thereby inhibit uptake of amino acids in a tor1+-dependent manner. In this study, we found that a temperature-sensitive allele of tor2+, tor2-ts6, was defective in uptake of leucine and conferred a resistance to canavanine. The two results are seemingly inconsistent.
In the fission yeast genome, ∼20 amino acid permeases are encoded, of which 11 permeases are activated at the transcriptional level upon nitrogen starvation. On the other hand, 5 are unchanged or even inactivated (Sanger Center Gene DB http://www.genedb.org/genedb/pombe/index.jsp and Nakase et al. 2006). It is likely that some permeases are required when the cell is starved for nitrogen. Other permeases, whose expression is not influenced by the availability of nitrogen, are responsible for uptake under actively growing conditions. The Tor2 kinase, as an essential component for growth, may be responsible for activating a set of permeases under actively growing conditions. A partial loss of function of tor2+ would thereby result in reduction of a total activity of uptake and consequently confer a resistance to canavanine. It is also possible that the Tor2 kinase may be responsible for regulation of a set of permeases, which are expressed at a basal level under actively growing conditions, but derepressed upon nitrogen starvation. Overexpression of tor2+ would result in suppression of the activity of these permeases and thereby confer a resistance to canavanine. Indeed, expression two permeases, Isp5 and 7G5.06, have been shown to be repressed in Δtsc2, a genetic background in which Tor2 kinase would be activated (Weisman et al. 2007).
The mechanism to control the uptake of amino acids is still to be elucidated on the molecular basis. In addition to regulation at the transcriptional level, the total activity of the uptake is likely regulated by stability and/or localization of individual amino acid permeases according to the nutrient condition. It was demonstrated recently that a loss of tsc2+ (Δtsc2) caused mislocalization of Cat1, a cationic amino acid permease, and that a mutation in the pub1+ gene coding a ubiquitin ligase suppressed the mislocalization in Δtsc2 (Aspuria and Tamanoi 2008). Pub1 would be a key component in determining the activity of the uptake at the post-translational level. Its functional relationship with Rhb1 and Tor2 is to be elucidated in the future.
Signaling by Rhb1 GTPase:
While amino acid uptake was prevented by both rhb1-DA4 and rhb1-DA8 in a dominant fashion, the response to nitrogen starvation was prevented only by rhb1-DA4. rhb1-DA8 thereby allows genetic dissection of the Rheb-dependent signaling cascade.
Although both overexpression of tor2+ and the rhb1-DA8 mutant causes resistance to canavanine, we speculate that they cause the resistance to the drug by independent mechanisms for the following reasons: (1) the synthetic lethality of the rhb1-DA8 tor2-ts6 double mutant suggests that the rhb1-DA8 mutant activates the Tor2 kinase only partially, and (2) the rhb1-DA8 mutant, unlike overexpression of the tor2+ gene, confers the resistance to canavanine independently from the function of Tor1. In addition, resistance to canavanine exhibited by a particular mutant could indicate that the mutant is tolerant to non-native proteins that have misincorporated canavanine. In this respect, we should not uniformly consider mutants resistant to the drug to be defective in uptake of amino acids.
The signaling cascade would branch below the Rhb1 GTPase or the Tor2 kinase. If it branches just below the Rhb1, the Rhb1 GTPase may regulate Tor2 and an element yet to be identified. Tor2, when stimulated by the Rhb1 GTPase, is responsible for suppressing the events adaptive to nitrogen starvation. The other one would be responsible for controlling amino acid uptake. While Rhb1-DA4 would constitutively stimulate both of these elements, Rhb1-DA8 would largely stimulate the one controlling the uptake. We demonstrated in this study that the Rhb1-DA8 GTPase could not stimulate Tor2 even in the Δtsc2 background, a condition under which the Rhb1-GTPase would exist as a GTP-bound form. Thus, the ability of the Rhb1-DA8 GTPase to stimulate its downstream elements may not be influenced by the guanine-nucleotide-binding status. It is rather likely that the Rhb1-DA8 GTPase may have an altered specificity toward its downstream elements and preferentially stimulate the one controlling the uptake of amino acids.
It is equally possible that the signal cascade branches below Tor2. The Tor2 kinase might exist as at least two different forms: one to support cell growth and suppress the response to nitrogen starvation and the other one to regulate the activity of a particular set of amino acid permeases at the transcriptional, translational, or post-translational level. Rhb1-DA8 may specifically stimulate the later form of Tor2 and reduce a total activity of the amino acid uptake. A partial loss of Tor2 suppresses a defect in the uptake of adenine caused by Δtsc2 (Matsuo et al. 2007). Another form of Tor2 might be involved in the uptake of nucleotides.
Tuberous sclerosis complex pathology:
Mutations in the human genes TSC1 and TSC2 are predisposing to a disease, tuberous sclerosis complex (TSC) (European Chromosome 16 Tuberous Sclerosis Consortium 1993; van Slegtenhorst et al. 1997). Because we showed that the rhb1-DA4 mutation mimics deletion of the tsc2+ gene in fission yeast, it is plausible that an analogous mutation in human Rheb would cause a symptom similar to that found in patients with TSC. Human Rheb could be a third TSC gene. Because our model study in fission yeast demonstrated that the phenotypes significantly varied depending on the allele, the symptoms of the Rheb-induced TSC could also be variable.
We thank M. Yamamoto (University of Tokyo, Tokyo, Japan) for strains. This work was supported by a grant from U. S. Army Medical Research and Material Command "Tuberous Sclerosis Complex Research Program" (to T. Matsumoto), by a grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to Y.H.), and by a grant from the Japan Society for the Promotion of Science (JSPS) (to Y.C.). T. Murai was a recipient of a scholarship from Teijin (Osaka, Japan) and a predoctoral fellowship from JSPS. Y.N. was supported by an exploratory research grant from JSPS.
↵1 Present address: Yamada Bee Farm, 194 Ichiba, Kagamino-cho Tomada-gun, Okayama 708-0393, Japan.
Communicating editor: O. Cohen-Fix
- Received May 22, 2009.
- Accepted July 16, 2009.
- Copyright © 2009 by the Genetics Society of America


text:
A lawyer obsessed with a curse that he believes has been cast over him and his family by the spectre of a Woman in Black engages a sceptical young actor to help him tell his terrifying story...
Continuing its record breaking run in London's West End, THE WOMAN IN BLACK now embarks on a major UK Tour Susan Hill's acclaimed ghost story comes dramatically alive in Stephen Mallatratt's ingenious stage adaptation. This gripping production, directed by Robin Herford, is a brilliantly successful study in atmosphere, illusion and controlled horror.
Captioned in house by Sheffield Theatres
Phone: 0114 249 6000
Email: firstname.lastname@example.com
Website: Sheffield Theatres


text:
Lauren Vidal Faux Leather Jacket
This variant is currently sold out.
Short-sleeved jacket in faux leather with a tie collar to tie and a metal zip in the front.
Fabric 1: 100% Polyurethane Fabric 3: 100% Polyester
Machine wash 30 ° c Chlorination forbidden Mechanical drying forbidden Iron at max 110 ° c Dry clean


text:
Temporary Closure - Shrine of Remembrance
In response to the spread of COVID-19, the Chief Executive Officer of Victoria's Shrine of Remembrance, Mr Dean Lee, has announced that the Shrine has closed its doors to public access - effective immediately and until further notice.
"Heightened anxiety and perceptions of risk amongst our patrons and personnel, reinforced by decisions by Museums Victoria, the State Library and NGV, have caused us to conclude that closure is in the best interests of the Shrine, our staff, volunteers and patrons." said Mr Lee.
The 2020 Anzac Day Dawn Service will proceed in some form however it is likely Victorians will be asked to commemorate on this important day by watching the Service on television, or via the Shrine's Facebook or Youtube channel.
Further information will be provided in the coming weeks. Updates will also be posted to the Shrine's Facebook page. or please visit the Shrine website: https://www.shrine.org.au/Home.
The Shrine of Remembrance will be closed to the public until further notice.


text:
While I was riveted by this big blooming beauty –
– pretty right? – it's called "4 o'clock," but I have no idea why because I've seen it blooming at all times – the twitch of an ear, perhaps, caused me to focus in on this, just in front of that:
Holy Jack, rabbit! Can you believe that eyeball?!
I was "just driving along when," by the way, and I took these pix right out the Jeep window. I'm glad I saw Jack before I opened the door and scared him away! (Or is it Jacqueline?) S/he did finally streak away – long ears flat back along his/her body in a way that reminded me, oddly, of a racehorse – but wow. I just can't get over that eyeball!
Not 10 minutes later, I was walking out to see a band when I saw Jack's cousin – Peter:
Or is it Petra? 🙂 And yes, the hole is home. See the one right in front of him/her under/behind the greasewood roots/branches? That's apparently the "front door." It was in the side of a shallow arroyo. Not too worried about flash floods, I guess, eh?
It's fun to see some of the basin's smaller residents from time to time!


text:
Guest Report
Posted: Sat Aug 16, 2014 1:28 pm
My mom and i stayed in the one bedroom apartment last December. The place is a tad bit small but cosy. The kitchen has all appliances and cutlery you will need, should you decide to cook. The bedroom has two full size beds, iron and ironing board, as well as cable TV. You also have WIFI access. Each apartment has its own private back porch, with a breathtaking view of the bucco reef. The yard also has designated sitting areas should you decide to eat or chill outdoors. There is a jetty just a stone throw away, where you can get fresh fish at around 10am. There is also a nice restaurant. Sunday school (a street party that takes place every Sunday in Buccoo) is within walking distance. Even though there is no security, the environment feels safe and the owners are right on the compound. Patsy and Sonny are very helpful and friendly. I highly recommend this place to anyone who wants to relax. I do advise that you have your own transportation, as buccoo is little out of the way for new comers. It is however worth it.


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Tag: half
Spain's first outdoor skydiving simulator opens in Malaga
The acting Mayor of Campillos Francisco Guerrero, the acting Councillor for Sports Miguel Ángel Herrera, Deputy Mayor Maria Antonia Dominguez, ... Read More
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Hotel History: Shepheard's Hotel in Cairo known for opulence, famous guests, and the military
Shepheard's Hotel history goes back 178 years when it was originally built by Englishman Samuel Shepheard in Cairo. It was ... Read More
Horror at fun fair: 28 people injured in merry-go-round collapse in Spain
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Top travel destinations: US trends for summer 2019
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Trump's regime rolls out new Cuba travel restrictions , bans educational, cultural trips
US Government announced new extensive restrictions on travel to Cuba by United States citizens, effectively banning most of educational and ... Read More
Hotel Bella Grace under new management
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Travel Trends Index: Steady April growth, but international outlook alarms
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Luxury Cruising: Silversea celebrates silver jubilee
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Jamaica Tourism Minister Bartlett new cooperation with President Clinton on Tourism Resilience
Alongside President and Secretary Clinton, Jamaica's Minister for Tourism, the Hon. Edmund Bartlett spoke today the ongoing 4th meeting of ... Read More
Research into child sexual exploitation urgently needed says NGO
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ILTM Asia Pacific 2019 confirmed as premier 'one stop' opportunity to meet region's top decision making agents
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text: This group is for all the people who love the beautiful Asian girls who are strong, fit, athletics, muscular, tough, rough and fighters. If at your turn you have some nice photos featuring beautiful Asian girls who are athletics, muscular, fit, strong, tough, rough and fighters you are invited to send these photos on the group. You are all welcome on this group and enjoy!


text:
Modern college students use their computers for just about everything — research, studying, entertainment, procrastination. Keeping this habit of use in mind, Velocity Micro has been designing PC's that are optimized for college life in every way. On the inside, it's got all the specs a student would ever need: Intel Core i5-3570K processor, 8GB of RAM, and an NVIDIA GTX 660 Ti video card built by EVGA. This powerful setup performed well in our benchmark tests, and should be sufficient for the needs of most students.
Its guts are great, but what really makes this PC a good choice for college students is its design. The enclosure is made out of machined aluminum, which makes it extremely light and easy to transport. Velocity Micro also put a lot of consideration into how the PC's components are arranged, and positioned them in a way that makes them easily accessible and simple to upgrade or repair.
Our review unit rang up at around $1300, but the standard model starts at $749, which makes it quite affordable when you consider its specs. Check out our full Velocity Micro Vector Campus Edition review for further details.


text:
Here are some scenes from Off-Ramp's trip to Dodger Stadium Tuesday for their home opener. They lost 4-2 to the Diamondbacks, but we had a blast anyway.
Listen to the audio for all of these segments and more ... including the ritual tasting of the Dodger Dog.
1. Ushering in a new usher
Listen to the audio to hear Andrea, a veteran fan services worker at the stadium, quizzing Andrew, who was working his first game as an usher. Andrew got "nearest bathrooms," "escalator," and "smoking area," but he didn't know where to go to find the "bacon stretcher."
2. The Dodgers' season and the mystery of micheladas
Matt "Money" Smith from the Petros and Money Show on AM570 gives us his take on the Dodgers chances this season, and wonders with me how people can drink micheladas, as in who ever thought, "Hey, let's pep up this bland light beer with clam juice!"
3. Up in the Nosebleeds
I went way up in the left field reserve section to talk with Mark and Shanna, father and daughter Dodgers fans who took their seats around 1030am ... even though first pitch wasn't until after 1pm. Turns out they were late last year and didn't want to risk it.
4. Dixieland Dodgers
The Angel City Dixieland Band has been doing the home opener for half a dozen years. They give us "Blue Skies" and "Take Me Out to the Ball Game."
5. Will the Dodgers ever be on TV?
LA Times columnist Chris Erskine says 60% of fans still can't watch the Dodgers on TV, but he optimistically thinks they'll nail down a deal in the next five years.
6. The The National National Anthem Anthem Anthem Anthem...
Sam Harris, lead singer for the Brooklyn-based X Ambassadors, nailed the National Anthem at the Dodgers Home Opener. He tells us how to beat the echo: good earphones.


text:
Enjoy and get this Maybelline and save up to 50% Off discount Second Buy straight from new York collection mix and match new & only available at watsons.hurry grab this now!
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• Age rewind dark eye circle eraser, erases all dark circle instantly $19.90
Promotion item must be of equal or lower value. Promotion valid till 1 July 2015 new York collection is only available at the following watsons outlets: bedok mall, braedtalk IHQ, bugis junction, city plaza, cleminti mall, compass point, Eastpoint, far east plaza, funan IT mall, hARbour front centre, houngang mall, heartland mall, hougang st 21, international plaza, ION orchard, jem, jurong point, jurong west BLK 960, marina square, NGee ann city, North point, Orchard gateway, parkway parade, people park, raffles city, revervale mall, tampines mall, tampines mart, tampines one, Toa payoh central, United square, vivo city, west gate, westmall.


text:
EVA SPEZIAL SANDWICH ® I Sport
- Product Number: 22668
For the efficient manufacturing of adapted foot beds for diabetics and rheumatics, to combine with an individual bedding cover layer.
Total Thickness
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Oven Settings
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Activation Time
4 min.
Cooling time
8 min.


text:
People with high cholesterol or those at high risk for heart disease and heart attack, making major lifestyle change may have probably been advised by the doctors in order to lower the level of cholesterol. Even with the changes in lifestyle, some still needed statin drugs which lowers cholesterol level. Dietary supplements are also recommended.
Top cardiologists do agree that supplements can be very good to keep a healthy heart. Some of the supplements which benefit the heart are:
- Plant sterols
- Fish oil
- Fiber (psyllium)
- Niacin
- Red yeast rice
- B-Complex vitamins (B6, B12, folic acid)
- Policosanol
- Green tea extract
- Coenzyme Q10
It is a must to check with your doctor before taking any supplements since may have contra indications with the other drugs you are taking. Pregnant women for instance are not allowed to take supplements as these may have effect on the pregnancy or breastfeeding. Make sure that the supplements are approved by the United States Pharmacopoeai or USP, which means that the active ingredients contained range from 95% to 100%.
Omega 3 Fatty acids, which are derived from fish oil, flaxseed oil and algae oil, significantly reduce triglyceride level and increase HDL (good cholesterol). Omega 3 has consistently shown to enhance the health condition of the heart. They have anti-inflammatory agents which can prevent diseases like Alzheimer's and heart diseases when inflammation is controlled. Numerous research studies have shown that regular eating of oily fish like salmon or taking of fish oil supplements for people with a history of heart attack have helped them reduce heart rhythm problems, heart attack and sudden death. Chest pains are also greatly reduced.
Fish oil supplements reduce triglycerides by 25% – 50%. The only thing to be cautious about is its mercury content. Only fish oil supplements tested free of mercury should be taken.
- Vitamin B3, nicotinic acid or niacin, is an effective supplement to counter high cholesterol. Proven to increase HDL and decrease triglycerides, this is a common prescription given to patients with blood pressure problems. Studies show that niacin can act better than statins in improving HDL cholesterol, increasing it by 15% – 30% and reducing triglycerides by 20% – 50%. Those who take niacin have shown no heart rhythm problems.
- Psyllium or ispaghula is derived from Plantago ovate husk seeds. Psyllium in supplement form or high-fiber foods can reduce total and LDL cholesterol. The effect of fibers on HDL or good cholesterol is not definitive although there are reasons to suggest that it may have helped improve HDL. 15 grams of Psyllium daily will reduce LDL by up to 9%. It enhances the effects of statins in lowering LDL cholesterol. Psyllium tends to affect the absorption of other medications so it is important that your doctor is consulted before taking this supplement.
- Red Yeast Rice is a specific yeast derived from rice. This is known to lower total and LDL cholesterol and triglyceride levels. It is also known to help in the prevention of heart attack and improve blood circulation. Red yeast rice contains monacolin K, a substance that has identical function to the active ingredient of statin drug Mevacor. Red yeast rice supplements work best with the right formulation. A beginning dose of 2400 mg a day should be tried for two months, afterwhich the cholesterol level should be checked for any changes. Your doctor needs to know the results after two months so he can recommend if there's any need to change dosage or formulation.
- Green Tea Extract, made from dried leaves of a perennial shrub Camellia sinensis, is a Chinese traditional medicine which is said to decrease LDL by 16%. A beginning dose of 375 mg of theaflavin-enriched green tea extract every day is recommended.
- Vitamin B6, B12 and Folic Acids are known as the B vitamins. They are known to lower blood level of homocysteine, an amino acid which may be linked to blood clots, heart disease, strokes and heart attacks. A doctor who has specialized understanding of lowering homocysteine with B vitamins should be consulted to avoid any complication concerning osteoporosis and cognitive decline which are prevented by homocysteine.
- Coenzyme Q10 is produced by the body to help with the cells basic functioning. Studies showed that CoQ10 (coenzyme Q10) reduce angina or chest pains and help people with blocked blood vessel. Some doctors would recommend CoQ10 for people taking statin drugs to help prevent muscle pains or damage to the liver which are direct results of taking statins.
Supplements are not the cure-all treatment for diseases. Given that they help in improving one's health, changes in one's lifestyle. A well balanced diet and a healthy lifestyle will all have positive effects on one's heart health.


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How I Optimised My Game In Unity
Last year I was working on a big RPG project as part of my studies. It looked great but didn't run so well on the ps4 dev kit. After a lot of tweaking I got it from a pretty stuttery frame rate to something smooth. This guide will tell you how I did it.


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- 1 night
1 Room, 1 Adult, 0 Children
Crowne Plaza Hong Kong Kowloon East
Hotel Reviews
2,821 reviews
Wi-Fi in designated areas
Airport pickup service
Hotel Location
- Tseung Kwan O450 m
- Tiu Keng Leng960 m
- Hong Kong International Airport46.94 km
- Hung Hom Station13.3 km
- Hong Kong West Kowloon Railway Station14.53 km
- City centre15.77 km
View Map
- 1 night
1 Room, 1 Adult, 0 Children
Hotel Description
- Opened: 2012
- Number of Rooms: 359
Services & Amenities
- Wi-Fi in designated areasFree
- Airport pickup service
- Car rental
- Luggage storageFree
- Front desk safe
- Valet parking
- Taxi booking
- Concierge service
- Attraction ticket service
- Wake-up call
- Credit cards accepted
- Express check-in/checkout
- Currency exchange
- Front desk (24 hours)
- Tourist mapFree
- Postal service
- Restaurant
- Café
- Room service
- Bar
- Elevator
- Executive floor
- Executive lounge
- Lobby bar
- Accessible rooms
- Non-smoking floor
- CCTV in public areas
- Meeting rooms
- Business services
- Fax/copying service
- Butler service
- Multilingual staff
- Wedding service
- Dry cleaning
- Ironing service
- Laundry service
- Outdoor swimming poolFree
- Mini shopping mall
- Spa
- Childcare Service
- Kids' club
Need to Know
Hotel Policy
- Check-in from 15:00
- Check-out before 12:00
Children are welcome at this hotel.
For each room, 1 child between 2-12 years old can use existing beds.
- Children between 2-12 years oldBreakfastNot includedFeeFree
Extra beds cannot be added
Max. 1 crib per room.
- Children 1 year old and underBreakfastNot includedFeeFree
Children aged under 18 years old must be accompanied by a parent or legal guardian. Children aged over 12 years old will be charged as adults.
Child and extra bed policies vary by room, each room rate is for a specific number of guests. Additional fees may be charged for accompanying children and additional guests. For details, please confirm with the hotel.
- MenuBuffet
- FeeHK$251.00 (Approximately AU$45.14) /person
- Parking is available (additional charge).
No pets allowed.
The following payment methods are accepted by the hotel:
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A set of 30 modern illustration kit.
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A set of 30 modern illustration kit.
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The Red Sox pitchers and catchers all officially reported to Ft. Myers yesterday, but there was one noticeable absence: Jason Varitek.
Speculation is running wild now that Tek will go the same route Tim Wakefield went last Friday: he will shortly announce his retirement from MLB.
And I hope he does. He has an invite to camp on a minor league deal, but the fact that he's not in right now tells me Tek's ready to hang it up.
He might just be waiting for some time to pass after Wake's announcement to make one of his own. We shall see.
Both Josh Beckett and Jon Lester spoke to the media yesterday about the terrible events of last September. Frankly, it's time to move on from 2011 and turn the page. I don't care about "apologies" or "mea culpas." It's a new season and I know the media will harp on this all spring and into the new season. They're the Boston media. They are expected to harp on the Red Sox failures.
My friend Chris Jaffe has another of those cool columns about a player's career highlights at The Hardball Times, and this one is about Tim Wakefield. Check it out here.