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What do the both of you do to stay so healthy and strong? " Practice is taken by asking questions in a way that is flirtatious. The first couple of times you will goof it up but don't quit trying. Every single time you attempt it, you'll get better at exclusive sex dating what to say and just how to say it.
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If you work at these steps, you are likely to create significant improvements. All you need to lose is your poor view of yourself. Make this part of the trail an important facet of your growth. This rebuilding block will affect aspects of your general lifetime.
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Look back at a few of your past communications. Examine emails that have more than two paragraphs. Two paragraphs are a limit to set for yourself. This does not imply that you are able to write two paragraphs of one thousand words each! While nevertheless being vague exchanges should be informational and short. Adam will. . . " And you also go on for six- to- eight sentences. Let him find out more about you and your family.
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I waited for the next day when we were in the chow hall and just began talking to her. Inform her. Kept the dialogue. She knew nothing. For us to hang out later on that evening and I sort of forgot about the date I intended. ( Got caught up with buddies performing hood rat military shit) But she was there waiting for me. Realizing what I had done had caused her to be even more curious now later on in life. I left her wondering.
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Final Thoughts on Dating a Local escorts backpage Lac Baker New Brunswick Player I have never been someone who plays with games and have always been exceptionally straightforward. As a result of this strategy, I've discovered a hypocrisy in men in regards to players. I have heard my whole life how men simply can't handle girls who" cause drama" or" play games" As soon as they get together with somebody who has better backpage escorts to do with her time, suddenly they don't know how to act. They start playing with their games to the alternatives to backpage escorts Lac Baker NB in the relationship. Then, months later, you'll find them hooked up that everybody in town calls a psychopath.
There is A Natural not conscious of exactly what he is, and therefore is not aware of his potential. A non- Natural, on the other hand, has every facet of this Inner Game under control and can aim to enhance himself at any moment.
Evidently, you will not be able to embrace every addiction. We suggest that you read through the entire book and make notes about customs that are pertinent to your Lac Baker New Brunswick or relationship goals. Select one habit at a time to concentrate on for four to six months or before the behaviors that are new feel automatic and natural. You may also need to maintain a journal to record your progress as well as your thoughts and feelings.
Next time you are browsing your preferred dating site, take a peek at five guys you might have passed up because they didn't fit your" what I want in a guy" test. Give yourself the opportunity to revisit them and see whether there is. You may find yourself resisting these men.
You are a man; you can't help but appreciate women that are alluring, right? Yeah, but blatantly checking out other women before your backpage escorts is not only considered disrespectful but will get you labeled as a player when you are not.
Lists can be about the things whatever they do for you that you appreciate they make you feel inside they are and other very personal but attentive particulars.
We do not observe this backpage escorts bbw Queensport NS's culmination, of getting our self with somebody else. We get ashamed, and we revert back to our shame and blame we will go blind or where we are advised to not touch it.
It ends up that it's actually pretty easy: you just Lac Baker backpage all escorts the aforementioned emasculating behaviours, turning them into masculating behaviours( ta- da, only coined a new word) . Because that's what it seems like when a woman does this sort of thing for us I call them gifts. We will go over those next.
They left what looked to be a glob of oil jelly petrifying before my eyes when they did pop off. My penance for all the lip- marks my mom had needed to wash away was a good ten minutes of Windex and a razor blade to eliminate the traces of my reversion.
Do not fall for it. Her pussy will never be wet for you as you want or as once a woman knows even for a second that she can conquer you emotionally. All of my writing is intended to give you an insight into the way without requiring any shit, that his achievement can be maxed out by a player for casual sex.
She's being told: " You can't get a guy by chasing him. " You need to: Know she is told this and do not take lack of pursuit because lack or rejection of interest. You stalk her and also shouldn't invent an imaginary connection. You also ought to know that a lady who pursues you is likely to wish to have sex with you, but you will not be curious about her since again, it was simple.
Now, recall, SerialKiller isn't my title for this guy, it's Sienna's. I do not really believe he's harmful although I admit, there are a few things in his past. ( nor do I believe Sienna does- our names for all these online guys are pretty arbitrary) Some of the things that initially held me back from agreeing to him will be removed as hurdles very shortly.
HOW TO Backpage all escorts Lac Baker NB THAI LADIES? Coming close to a Thai girl truly isn't tough as a lot of are open and really pleasant, you will remain in truth surprised how swiftly they will certainly begin a good discussion with you the minute you satisfy them. Nevertheless, do not take their visibility to be an open invitation to the bed! Yes, they are pleasant but that does not imply that they want sleeping with you or anything. The Thai society places a substantial quantity of pressure on the women to show up chaste as well as moderate and Thai women go to wonderful lengths to preserve their social dignity.
There are times when you'll have to hold off securing a date since you've got too much on your plate. Hold communicating off and reopen, whenever you have the opportunity.


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TEDxGatewayWomen2015 comes to Mumbai on May 29th
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Momentum. (n) The strength or force that allows something to continue or to grow stronger or faster as time passes.
As human beings, we were made to move. Designed to be in a continuous state of motion, our minds and bodies are defined by ideas that create momentum in how we live, think, and work.
Exploring the theme that every change begins with momentum is the first-ever edition of TEDxGatewayWomen2015 being organized on May 29th, in Mumbai. Taking the cue from the global TEDWomen2015 program on May 27-29, in Montery, CA, TEDxGatewayWomen2015 will put the spotlight on the power of women as ambassadors of change, from small, local solutions to big, global movements.
YourStory is a Media Partner for TEDxGatewayWomen2015
Guided by the renowned global TED format, the event will bring together an impressive line up of women speakers on one platform for talks ranging from 12-14 minutes each. From the Canadian artist who will take the audience on a journey of past, present and future and the dancer whose moves cured her cancer, to the farmer seeking to empower low-income groups through economic activity, and the publisher who will talk about how one idea that comes out of curiosity can brighten up the world for many, the day-long conference will focus on the unique way in which women contribute to global change and thus help reshape the future.
Some of the speakers include Reemaben Nanavaty a Padma Shri awardee who will be sharing her stories of work, on empowering two million women to overcome poverty calling it 'Doosri Azadi'. Deepika Bharadwaj is another speaker who will discuss on the true sense of equality and Aditi Gupta of the Menstrupedia fame.
As staunch supporters of the idea that the world needs more platforms for inspiring women to be heard, and encouraged, we at YourStory are happy to partner with TEDxGatewayWomen2015 to bring our readers exclusive coverage of the event, and stories of the inspiring speakers.
We're also giving five of our readers an opportunity to win a free pass for the event. TEDxGatewayWomen curates its audience to select a diverse group of people who can contribute meaningfully to the conversation. Just like the selection of diverse speakers from across several disciplines, you could be part of an audience comprising change agents from the fields of politics, sciences, technology, creativity, banking & finance, industry, philanthropists, government, arts, and medicine, etc.
To win a pass, simply write in and tell us 'Which woman speaker are you looking forward to hearing the most?' In other words, from the speaker lineup, whose story inspires you the most?
Send your entries here.
For a complete list of the speakers, click here.
Other Details
Date and Time: May 29th, 2015 – 1PM onwards
Venue: Tata Theatre at NCPA, Mumbai
We are waiting to hear from you
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Microbiology and infection / T.J.J. Inglis.
By: Inglis, T. J. JMaterial type: TextSeries: Master medicinePublisher: Edinburgh : Churchill Livingstone, 2003Edition: 2nd edDescription: viii, 294 p. : ill. ; 27 cmISBN: 0443070954Subject(s): Infection | Medical microbiology | Communicable Diseases -- microbiology -- Examination Questions | Infection -- microbiology -- Examination QuestionsDDC classification: 616.01
|Item type
|Current location
|Call number
|Date due
|Item holds
University College of Medicine Library
Shelf No. 33, LBS
The library of the University College of Medicine & Dentistry was established in 2001 with the establishment of The University College of Medicine & Dentistry, the constituent College of The University of Lahore (UOL). The Library is housed at new campus of The University of Lahore, Defense Road Lahore. The Library has a rich up-to-date collection of books, CDs/DVDs, International Impact factor journals, national periodicals, newspapers, Digital Library and has access to the digital information resources subscribed by UOL.
The mission of the Medical Library is to be a highly effective member of the UOL learning community, providing leadership in information mastery, access to essential spaces, resources and services, and collaborating in the development and delivery of an innovative medical education.
|Not for loan and check out is restricted
|616.01 ING-M 2003 502851 (Browse shelf)
|Not For Loan (Restricted Access)
Includes index.


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35 Followers
Explore 15+ million radio shows, DJ mixes and podcasts made by passionate creators.
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This upload features tracks from Summer Camp, Elite Gymnastics and more.
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Simon Patterson - Digital Showdown - 008
by Simon Patterson
Don't Stay In Mix of the Week Volume 075 - Claudia Cazacu (electro/techno/trance)
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The Feddie Reporting Award
Christina Jewett 2019
Kaiser Health News
Christina Jewett is a senior correspondent with the Kaiser Health News enterprise team. She spent seven years with the Center for Investigative Reporting, where she worked with a partner and CNN on a series that uncovered widespread graft in Medicaid-funded drug rehab centers, spurring the closure of scores of centers and 11 arrests. She and colleagues won a George Polk Award for medical reporting, writing about a hospital chain that billed for an outsized rate of rare and lucrative ailments. Her work on the Food and Drug Administration's hidden medical device database was also honored with a Barlett and Steele gold award. She previously worked at ProPublica and The Sacramento Bee. She is a graduate of Indiana University.


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In that video we name a few people who Gracie trained with. Anadrol has an anabolic presence 3 times more powerful than testosterone. Inpatient recovery: You can live at the facility, away from your using environment, in order to focus on your recovery. Updated by Paula Vauhkonen Licentiate of Medicine (Lic. Anabolic steroids may be addictive, and users may go through withdrawal. Boost in IGF-1 Insulin-like buy Melanotan 2 tanning injections Growth Factor (IGF-1) sends amino acids and glucose to the muscles so that they grow. The physiological direct effects of testosterone and AASs (AR-mediated) are well known. On the contrary, Mexican legislation is much softer with regard to anabolic steroids. Representatives of various sports are very quickly appreciated all the advantages of Oxan and began to apply it on a regular basis in their steroid cycles.
In exchange for wire money transfers to sources located in Qingdao, China, Bryan and April Wilson would receive raw steroid powder. On the protein front, my favorite thing to do is add 10 grams of branched chain amino acids to the mixture. Make no mistake, Primobolan has its place in a performance capacity, but most men will find the Depot version to be a better call. Pay attention to your urine frequency, and if it seems as if emptying your bladder becomes difficult or painful, stop using Sustanon steroids. High dosages are associated with increased risk of side effects.
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Expert film critics Erik Childress and Steve Prokopy round up what you should (and shouldn't) be watching.
This week, Erik and Steve bring you their thoughts on VOD releases of Scoob!, Castle in the Ground and Capone. Plus, catch up on some animated films that are great for the whole family and some just for the grownups.
To download this or any of Nick's podcast visit our iTunes page. Like us on Facebook (/nickdshow) and follow us on Twitter (@nickdshow.)


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A year ago the Twins were marching toward an eventual home run record, clubbing more bombas, 307, than any team in MLB history. It was clear early on in the year that the team was special, though it's tough to pinpoint when we collectively knew it was that Special.
Here's one column from On This Date, April 29, in 2019. With some added commentary. For context, the Twins had just finished a 3-game home sweep over the terrible Orioles. They were headed to Houston, where Jake Odorizzi would lock into a pitcher's duel with future Hall of Famer Justin Verlander.
MINNEAPOLIS – The Twins have clubbed 49 home runs so far this young season, and they'll probably get to 50 before they run out of games in the month of April. It's an incredible pace bested only by the world-beating Seattle Mariners in the American League. (Seattle has played 6 more games than Minnesota, so look out, M's.)
The home runs have come at such a frantic pace that the Twins have put themselves in position as one of the best offenses in the A.L., and the only hiccup on the schedule to date was a rocky 4-game series with the Toronto Blue Jays.
This winter there was some talk among a certain segment of the fanbase that the Twins were taking a risk, as they loaded up their roster with some new hitters that hung their hats on hitting it over the fence, rather than getting on base at a high clip. That list of hitters includes Nelson Cruz, C.J. Cron and Jonathan Schoop. In the early stretches anyway, the team's front office appears to have placed a good bet.
[Good point! Prescient, really.]
This column presents 5 thoughts on the home run parade currently being performed by the Twins' lineup:
Actually, those 11 home runs lead the American League, as of this writing.
Do I think Rosario will win the A.L. home run race? In a word, no. But let's not ignore the fact that Rosario already has reached double digits and we're only a month into a 6-month season. Here's the Twins' leaderboard after the weekend sweep of the hapless Orioles:
Eddie Rosario, 11
Max Kepler, 7
C.J. Cron: 5
Nelson Cruz: 5
Mitch Garver: 5
Jorge Polanco: 5
Jonathan Schoop: 4
I expect some jostling at the top of that list. By the end of the year, I think you'll see Cruz's and Cron's names at or near the top of Minnesota's leaderboard, and don't be surprised if Mitch Garver hangs around in that race, too. Rosario and Kepler will continue to appear on the Twins leaderboard, although this is a new level of power from Rosario that we have not seen to this point in his career.
[I think it was deemed a hot take at the time when I said Garver would hang around. Dude rakes.]
Kepler hit another home run on Sunday – on the first pitch thrown to the Twins, no less – which was the exclamation point on a power outburst for the newly extended right fielder. He hit 2 homers in a win on Saturday, and he also got on the board Friday against Baltimore. There's a guy who's probably sad to see the Orioles get on a plane out of town.
We'll have plenty more in the coming days and weeks about the bet that the Twins made on Kepler, "pre-breakout," but let's at least point out right now that after a month, the 26-year-old's batting line is far better than his career norms. That's especially true in the power department. Kepler is hitting .274/.340/.571, which is a nice jump in average and on-base percentage from last season, but it's a monumental increase in power production.
Kepler led off with the homer off Dylan Bundy on Sunday and I asked him about a topic that's near to his manager Rocco Baldelli's heart: quality at-bats. Every time Rocco is asked about the homer surge, he continues to underscore that he's more concerned about the consistent quality of the at-bat, rather than the result of the ball leaving the yard.
"Yeah, I've always said quality at-bats is key over anything, when it comes to hitting," Kepler said after his 4-homer weekend. "Whether it's working an at-bat – as the leadoff, working a good long at-bat – or hitting a good pitch, first pitch. There's multiple ways you can have a quality at-bat and I think this team's been having a lot so far."
The guy who leads the A.L. in doubles (12) found a way to put two together in one plate appearances Sunday against Orioles pitching. He left the park for the first time of the season, and the early signs offensively have been encouraging for the player with the widest variance in the organization.
The doubles, the loud contact, and the home run all register as checks on the good side of the lingering question, Is It For Real This Time?
To answer that, I would say that he's not out of the woods yet. You'd rather have this great start at the plate than not have it, it's just that for right now let's say he's had a good month and stop there.
He's hitting .270/.325/.500 with 12 doubles and a homer. That's a good batting line for a player who legitimately is on the short list of best defenders in baseball. He's also trending in the good direction with more advanced metrics, like his hard-hit rate, the speed he hits baseballs, and how often his swings find a pitch with the barrel of the bat.
For what it's worth, Buxton does rate in the top 10% in the Majors in average exit velocity, at about 93 mph. He's hit 20 different balls at a speed of at least 100 mph, including a string of 5 scorchers in a row two weeks ago against the Tigers and Blue Jays.
Let's hold off crowing him a superstar just yet. The encouraging thing for the Twins and their fans is that Buxton is looking much more fit for the part than he was six months ago. And now he's on the board.
[Relevant link from around that time: Byron Buxton leads the Majors in doubles, and that matters.]
José Berríos leads the staff with homers permitted (6), but he's also been the innings hog that the Twins want him to be this season. When you control for the numbers of frames pitched, Berríos and Michael Pineda are pretty close in overall effectiveness of shutting down long balls.
We like to complain about the pitching staff around here, and some of that is warranted. It's just worth noting that when the bats outpace your hurlers in a month with 49 homers to 29 for the staff, your club is doing something right.
[Runs are runs, is what I'm saying.]
Better than those two, Jake Odorizzi (Monday's starter) and Martin Pérez have kept the ball in the yard nicely, and if you're the Twins you'd hope those trends continue. In Pérez's case because everything looks good so far about their bet on his remaking himself as a starter in his late-20's; and in Odorizzi's case it's because his approach in the past has meant that when he struggles on the score sheet, it's often because those high fastballs turn into homers more often than he'd want. Good things from both of those starters through the first month of the season.
On the reliever front, homers help tell a story, too. On one hand you've got Chase De Jong and Tyler Duffey, who rate near the top of the Twins in homers-surrendered-per-batter-faced. Those long balls – and the circumstances of their promotions – helped to earn them a trip back to Triple-A Rochester. On the optimistic side of the Twins bullpen worries, the four guys that you need to be good have been that. Among the late-inning quartet, only Taylor Rogers has served up a gopher ball, and even that shouldn't have been on his books. That was the deep fly ball to center field that stayed in the park and all-world defender Byron Buxton turned into an inside-the-park home run. Blame Rogers for the loud contact, but after that he's not in control of whether that ball is an out, a double or a little league home run. Trevor May, Trevor Hildenberger and Blake Parker have yet to allow a home run this season, through Sunday.
[Whoops I guess I missed on Future Tyler Duffey blossoming into a relief ace.]
Home runs are up so much in the first month of the season, it's led some humans to speculate that the baseball is once again "juiced."
Here's a glance at the league-wide trend through April 27 each of the past 4 years:
|MLB Home runs
|Plate appearances
Source: FanGraphs.com (totals through April 27)
The total home run count will be topped this year, I'm guessing. Some are speculating that the single season home run record is in danger. (Note: Do you think MLB would like to take that belt from Barry Bonds and hand it over to a new heavyweight champion of the world?)
[Well, Bonds still holds the belt. But a new Home Run record was set, and it was set by the Twins.]
Bringing in Cruz at Cron at moderate price tags, and even Jonathan Schoop, relative to his peers at the position, looks a little bit like a bet on power over OBP.
[In hindsight, I'd probably amend this to say that the Twins made a value play on the power over OBP, but that's a minor nitpick with One Year Ago Derek Wetmore.]
Now, roster decisions are not made in a vacuum, and the goal of the Twins front office, I've been told, is more ambitious than to win the single-season home run crown. Still, it's fascinating to me that the Twins have morphed their roster in this way at a time the baseballs seems to be flying like never before.
Manager Rocco Baldelli said it doesn't change how they build a roster or manage an individual game.
"Not directly, I don't think [it changes anything]," Baldelli said. "We're just trying to put the best lineup out there today to win the game – looking small-picture at today, and looking big-picture – that's how we'll assemble our roster. We look at it a week out and try to figure out how it all fits together. But I don't think the home runs that either we're hitting or that are being hit throughout the league really change any of that."


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In his El Heraldo newspaper column, WBC President Mauricio Sulaiman announced that from now on only world championship and silver championship fights will be sanctioned for the twelve round distance. Fights for other WBC titles will be contested over ten rounds or even eight rounds. This was approved unanimously by the WBC Board of Directors, based on the recommendation of the WBC Medical Committee.
In 1983, the WBC under the guidance of Mauricio's father José Sulaiman reduced the length of world championship fights from fifteen rounds to twelve rounds. Now 37 years later, the WBC has taken the next step to protect boxers.


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Intellectual Property Law: Size Matters for TVs on Super Bowl Sunday
A familiar question dependably re-emerges at about this time each year. It essentially asks "can I get in copyright trouble for having a Super Bowl party with a big screen TV?" This post gives you some ground rules in order to spare everyone from the possibility of being arrested by the copyright police . (Disclaimer:…Read More
Intellectual Property Law: An Overview of Custom Software Ownership
Companies sometimes pay large sums of money to independent contractors to create a custom software program and mistakenly assume that they own the result. Many companies have valuable intellectual property developed within their business, one of which is rights in copyrighted materials. Copyrights do not involve just novels and artwork; they can include software programs,…Read More
University of Alabama seeing Crimson over recent ruling?
In University of Alabama v New Life Art, the 11th Circuit Court of Appeals resolved a portion of a long standing dispute over the protection afforded authentic reproductions of college uniforms. A painter made paintings (and calendars) accurately depicting University of Alabama games. For many years this practice was unlicensed, but done with the knowledge…Read More
Recent court decision in Oracle v Google raises serious copyright questions in certain types of software
In a decision in the Oracle v Google case, the court held that APIs – application program interfaces – small amounts of human readable source code, are not sufficiently original to qualify as copyrights. This decision can impact API licenses, which most likely are based on copyrights. What Google did. Google decided that to construct…Read More


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Working with Peace Corps volunteer Joan Mutinda, together we have completed a huge number of projects in Mtae that have supported the local health centre, the secondary school and the primary school.Read more →
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- © 2020 American Society of Plant Biologists. All Rights Reserved.
In previous work, we identified a triple mutant of the castor (Ricinus communis) stearoyl-Acyl Carrier Protein desaturase (T117R/G188L/D280K) that, in addition to introducing a double bond into stearate to produce oleate, performed an additional round of oxidation to convert oleate to a trans allylic alcohol acid. To determine the contributions of each mutation, in this work we generated individual castor desaturase mutants carrying residue changes corresponding to those in the triple mutant and investigated their catalytic activities. We observed that T117R, and to a lesser extent D280K, accumulated a novel product, namely erythro-9,10-dihydroxystearate, that we identified via its methyl ester through gas chromatography–mass spectrometry and comparison with authentic standards. The use of 18O2 labeling showed that the oxygens of both hydroxyl moieties originate from molecular oxygen rather than water. Incubation with an equimolar mixture of 18O2 and 16O2 demonstrated that both hydroxyl oxygens originate from a single molecule of O2, proving the product is the result of dioxygenase catalysis. Using prolonged incubation, we discovered that wild-type castor desaturase is also capable of forming erythro-9,10-dihydroxystearate, which presents a likely explanation for its accumulation to ∼0.7% in castor oil, the biosynthetic origin of which had remained enigmatic for decades. In summary, the findings presented here expand the documented constellation of di-iron enzyme catalysis to include a dioxygenase reactivity in which an unactivated alkene is converted to a vicinal diol.
Di-iron clusters within the active sites of enzymes facilitate the binding of molecular oxygen and its derivatives and are able to perform redox chemistry, which results in a range of chemical outcomes (Edmondson and Juynh, 1996). All di-iron enzymes characterized to date belong to one of two separate classes, one soluble and the other membrane-bound (Shanklin and Somerville, 1991). Both classes have the ability to catalyze the oxidation of unactivated C–H bonds to give a range of chemical outcomes (Shanklin and Cahoon, 1998; Fox et al., 2004). For instance, both soluble and membrane di-iron enzyme classes contain desaturase enzymes that perform the stereo- and regioselective introduction of Z- (cis) double bonds into unactivated lipid acyl chains. The reactions are thought to proceed via a radical mechanism initiated by abstraction of a specific hydrogen from substrate (Buist, 2004). Double bond formation ensues via the abstraction of a second neighboring hydrogen. As predicted by Bloch (1969) and subsequently confirmed by x-ray crystallography (Lindqvist et al., 1996; Bai et al., 2015), the boomerang shape of the substrate binding channel within the desaturase drives the formation of the (Z)-olefinic fatty acids.
There is a diverse constellation of chemical outcomes performed by variant enzymes that are structurally related to the prototypical desaturase. The membrane-bound di-iron–containing plant fatty acid desaturase (FAD) family of FAD2 variant enzymes perform a variety of chemical transformations. Using oleate as substrate, either desaturated or hydroxylated products are obtained; using linoleate as a substrate, the corresponding epoxide, a conjugated double bond, or an acetylenic bond can be produced. Changes in chemoselectivity are based on a relatively small number of amino acid sequence differences that presumably alter the relative orientation of the substrate with respect to the active site oxidant (Bhar et al., 2012). For instance, changes to only four amino acid side chains was sufficient to predominantly convert a FAD2 into a hydroxylase and vice versa (Broun et al., 1998; Broadwater et al., 2002). Despite our increasing understanding of specificity determining residues within the FAD2-related di-iron enzymes, further interpretation has been hindered by the lack of structural information for these enzymes. Recently published structures of several mammalian membrane-bound desaturases suggest it will be possible to solve one of the plant FAD2 class at some point and we will be able to correlate changes to the enzyme structure with distinct functional outcomes (Bai et al., 2015; Wang et al., 2015).
The soluble class of desaturase enzymes exemplified by the castor (Ricinus communis) Δ918:0-ACP desaturase (Lindqvist, 2001) has been shown to contain members that display a variety of chain-length specificities and regioselectivities (Shanklin et al., 2009). Mechanisms have been proposed for both chain length specificity (Cahoon et al., 1997; Whittle and Shanklin, 2001) and for regioselectivity (Guy et al., 2011). During our studies on regioselectivity, we engineered a triple mutant of the castor acyl-ACP desaturase (T117R/G188L/D280K) that converts stearoyl-ACP into an allylic alcohol transisomer (E)-10-18:1-9-OH via a (Z)-9-18:1 intermediate (Whittle et al., 2008). This work described a soluble desaturase acting as an olefin oxygenase similar in behavior to that displayed by another soluble di-iron protein, methane monooxygenase (Gherman et al., 2004). We showed that the conversion of (Z)-9-18:1 substrate to (E)-10-18:1-9-OH product by castor desaturase T117R/G188L/D280K proceeds via hydrogen abstraction at C-11 and highly regioselective hydroxylation (>97%) at C-9 (Whittle et al., 2008). The 18O-labeling studies show that the hydroxyl oxygen in the reaction product is exclusively derived from molecular oxygen.
This work was initially designed to evaluate the individual contributions of T117R, G188L, and D280K in castor desaturase to allylic alcohol formation. During these experiments, we discovered a novel dioxygenase reactivity of the soluble desaturase that results in the conversion of oleoyl-ACP to erythro-9,10-dihydroxystearate. The same product was found in TMS-derivatized methyl esters from castor seed where it constitutes ∼0.7% of the total fatty acids
As part of our continuing structure–function analysis of di-iron enzymes, we analyzed the contributions of each of the mutations within the castor desaturase T117R/G188L/D280K triple mutant that converts oleoyl-ACP into (E)-10-18:1-9-OH (Whittle et al., 2008). Each of the individual mutants was constructed and tested for its activity using oleoyl-ACP as substrate. In each case, the product profiles were determined by gas chromatography–mass spectrometry (GC–MS) analysis. The results are shown in Figure 1. The GC elution profile of substrate is shown in Figure 1A and features a peak corresponding to 18:1Δ9 methyl ester (peak 1). A minor shoulder peak can be attributed to 18:1Δ11 (peak 2) and is a well-known artifact of the expression system. As shown in Figure 1B, the triple mutant T117R/G188L/D280K converted most of the oleoyl-ACP substrate into a mixture of the Z(cis)18:1Δ10 9OH (peak 3) and E(trans) 18:1Δ10 9OH allylic alcohol (peak 4) isomers, with the E form predominating by ∼3-fold over the Z form.
Reactivity of the Castor Desaturase Single Mutants T117R, G188L, and D280K
Each of the single mutants was active with respect to the oleoyl-ACP substrate (Fig. 1, C–E). The T117R mutant produced ∼15-fold more of the E 18:1Δ10 9OH isomer than the corresponding Z isomer. However, a new peak (labeled "5" in Fig. 1C) became apparent at an elution time that was not characteristic of the silylated derivatives of commonly occurring fatty acid methyl esters. The G188L mutant produced an ∼1:1 mixture of E and Z isomers of 18:1Δ10 9OH (Fig. 1D), but no detectable trace of the novel fatty acid species (peak 5). The D280K mutant was less active than T117R and G188L, producing only a small amount of the E isomer of 18:1Δ10 9OH (Fig. 1E), along with a small amount of the novel fatty acid (peak 5). As expected, the wild-type desaturase showed very little activity with its natural product oleoyl-ACP, but close inspection revealed the production of a trace of novel species (peak 5) based on its elution time and mass spectra (Fig. 1F).
The Novel Fatty Acid Product (Peak 5) Is 9,10-Dihydroxystearate
Mass-spectral analysis of the peak-5 product produced by the T117R mutant (Fig. 1C) revealed a molecular ion of 474 AMU, consistent with an 18C fatty acid methyl ester containing two silylated hydroxyl groups (Fig. 2A). Fragmentation of the product between the two silyl groups produced fragments of 259 AMU for the carboxyl-containing fragment and 215 AMU for the methyl-containing fragment (diagrammed in Fig. 2B), consistent with the presence of vicinal hydroxyl groups at C9 and C10. The identity of the peak-5 product was confirmed by comparison of its fragmentation pattern with that of a silylated authentic commercial standard of erythro-methyl 9,10-dihydroxy stearate (Fig. 2C). Analysis of the peak-5 product from the D280K mutant also showed the same fragmentation pattern.
9,10-Dihydroxystearate Produced by the T117R Mutant Is Solely in the erythro Configuration
Fatty acids containing vicinal midchain hydroxy groups may exist as threo or erythro diastereoisomers (Fig. 3). To distinguish between these possibilities, we compared the GC elution times of the novel product from T117R with those of authentic threo- and erythro-9,10-dihydroxystearate standards (Fig. 4, A–C, respectively). The T117R product eluted as a single defined peak without any detectable shoulders (Fig. 3A) and coeluted with authentic erythro standard (Fig. 4C). The authentic threo standard (Fig. 4B) eluted ahead of that of the T117R product (Fig. 4A). When a small amount of the T117R product was mixed with either the threo standard (Fig. 4D) or the erythro standard (Fig. 4E), two peaks were seen for the sample spiked with threo standard whereas a single coeluting peak was seen for the sample spiked with erythro standard. These results confirm the assignment of the T117R product as erythro-9,10-dihydroxystearate.
The Hydroxyl Oxygens at Both C9 and C10 Are Derived from Molecular Oxygen
The oxygen atoms in either of the two hydroxyl groups could in principle arise from water or molecular oxygen (Fig. 5). To distinguish between these possibilities, T117R, oleoyl-ACP, and all assay components were first degassed by multiple gas exchange cycles employing vacuum and O2-free argon with the use of a Schlenk line (Arnold and Bohle, 1996) to remove residual atmospheric 16O2 from the sealed reaction vials. Assay reactions were subsequently incubated in the presence of 16O2 or 18O2. We used mass-labeled 18:1 d2-11,11 oleoyl-ACP for these assays to ensure the product we observed was derived from the enzymatic reaction rather than from endogenous oleate contaminant. Analysis of the methylated silylated products from reaction under air yielded the expected 217 and 259 AMU products (the methyl fragment increased by 2 AMU relative to unlabeled product results from the substitution for the two hydrogens at C11 for deuterons; Fig. 6). The same experiment performed under 18O2 resulted in the production of fragments of 219 and 261 AMU, consistent with the incorporation of one 18O at each of the hydroxyl positions.
The Formation of 9,10-Dihyroxystearate from Oleate Is the Result of a Dioxygenase Reaction
The incorporation of molecular oxygen at the 9 and 10 positions of oleate could in principle result from a single dioxygenase reaction, or from two sequential monooxygenase reactions. To distinguish between these possibilities, we degassed the samples as described above and performed a reaction under an atmosphere containing an equimolar fraction of 16O2 and 18O2 (Fig. 7B) and performed MS on methylated acetonide derivatives of the product (Fig. 7E). Acetonide derivatives were used because they protect vicinal hydroxy groups while maximizing the detectable mass ion of the product. If the reaction operates via a dioxygenase mechanism, then the oxygen atoms at both hydroxyl positions should derive exclusively from either 16O2 or 18O2, resulting in either M or M+4 species. Alternatively, if the mechanism employs two sequential monooxygenase reactions, a 1:2:1 pattern of M:M+2:M+4 would be expected by random incorporation of either 16O or 18O at each hydroxyl position. Consistent with a dioxygenase mechanism, reactions performed under an equimolar mix of 16O2 and 18O2 yielded only M and M+4 peaks (355 and 359), with no detectable 357 species (Fig. 7B). Individual control 16O2 and 18O2 reactions only showed the expected 355 and 359 major species accompanied by minor peaks at M+1 and M+2 that approximate the natural abundance of 13C (Fig. 7, A and C, respectively). That M+1 and M+2 peaks originate from natural 13C was confirmed by the fragmentation of equivalent derivatives of an authentic erythro-9,10-dihydroxystearate, which showed the same proportions of M, M+1, and M+2 species (Fig. 7D).
The Native Castor Desaturase Can Convert Oleoyl-ACP to 9,10-Dihydroxystearate
The formation of dihydroxystearate with selected mutated desaturases prompted us to probe for the formation of this compound by the wild-type enzyme. Interestingly, using a prolonged time of incubation (240 min) with oleoyl-ACP as substrate, we were able to identify production of 9,10-dihydroxystearate (peak 5) at low levels (Fig. 8). This compound was accompanied by lesser amounts of E 18:1Δ10 9 OH (peak 4).
Castor Oil Contains Erythro-9,10-Dihydroxystearate
The observation that the native castor desaturase can produce small amounts of 9,10-dihydroxystearate correlates well with an early report by King (1942), who isolated a small amount of 9,10-dihydroxystearate from castor oil. We sought to confirm this observation and analyzed a fatty acid extract of castor seeds by GC–MS after methylation and silylation. Chromatograms of castor seed fatty acid derivatives (Fig. 9A) showed the expected common C16 and C18 fatty acids, along with a major peak of ricinoleic acid that is followed by a small discrete peak (labeled "8" in Fig. 9A, inset) of ∼0.7% (of total fatty acids), which corresponds to the elution time of disilylated methyl 9,10-dihydroxystearate. Mass spectral analysis of this peak revealed fragments of 215 and 259 AMU confirming its assignment as 9,10-dihydroxystearate (compare Fig. 9B with Fig. 2, A and C). Based on the in vitro assays using purified enzyme reported above, we hypothesize that this 9,10 dihydroxystearate arises from the dioxygenation of oleoyl-ACP product of the stearoyl-ACP desaturase. If this were the case, the 9,10-dihydroxystearate would be in the erythro form as originally proposed (Morris and Crouchman, 1972). We therefore conducted coelution studies with authentic threo or erythro standards (Fig. 9, C–E). The 9,10 dihydroxystearate isolated from castor eluted as a single peak (Fig. 9C) with the same mobility as that of the authentic erythro standard (Fig. 9E). By contrast, two peaks were seen in the spiking experiment using the threo standard (Fig. 9D).
Stereoselective dihydroxylation reactions are important to the chemical industry (Borrell and Costas, 2017) because diols serve as valuable synthons. The osmium-based asymmetric dihydroxylation reaction (Crispino and Sharpless, 1993) is a prominent example of controlled olefin oxidation and was (in part) recognized by the award of the 2001 Nobel Prize in Chemistry to its inventor, Karl B. Sharpless. In addition, biocatalytic diol formation from aromatics by whole-cell mutant Pseudomonas cultures has furnished the synthetic chemist with a variety of enantiomerically pure cyclohexadiene-cis-diols. (Hudlicky and Thorpe, 1996). Much effort has also been expended to develop iron-based biomimetic catalytic methodology for this reaction (Oloo and Que, 2015). Herein, we report the details of our investigation into a "green chemical approach": the castor Δ918:0-ACP desaturase-mediated syn-dihydroxylation of an unactivated alkene in the form of oleoyl-ACP to erythro-9,10-dihydroxystearoyl-ACP.
Stearoyl-ACP desaturase belongs to the nonheme di-iron subclass of oxidative enzymes that have been shown to mediate a variety of chemical transformations including dehydrogenation and mono-oxygenation. Typical products include primary, secondary, and allylic alcohols in addition to the conversion of double bonds to epoxides (Wallar and Lipscomb, 1996). However, a di-iron center performing dioxygen chemistry to convert a double bond to a vicinal diol as reported here is without precedent. The closest comparable example we are aware of is arylamine oxygenase (CmlI) from the chloramphenicol biosynthesis pathway, which incorporates two oxygens from O2 into the aryl‐nitro product; however, this occurs in two consecutive mono-oxygenations (Komor et al., 2017). We envision the conversion of alkene to vicinal erythro-diol in this work to be mechanistically related (Fig. 4) to that described for Rieske cis-diol-forming dioxygenases (Ensley et al., 1982; Karlsson et al., 2003). More specifically, we envision involvement of a bridged hydroperoxo-di-iron species similar to that proposed by Solomon and Srnec (Chalupský et al., 2014) for the conversion of stearate to oleate by two consecutive hydrogen atom abstractions: "-CH2-CH2-" to "-CH=CH-." When presented with an alkene moiety, the vinyl hydrogens are unavailable for abstraction for steric reasons and this same species is forced to transfer two oxygen atoms to substrate as shown in Fig. 4 (Pathway 1). Our oxygen-labelling experiments rule out an epoxidation/hydrolysis route (Pathway 2). It is possible that our T117R mutant may change the molecular architecture of the substrate binding cavity, altering the relative orientation of the substrate with respect to the hydroperoxo-di-iron group and facilitating deoxygenation relative to the wild-type enzyme. That the diol is produced as the erythro diastereoisomer, in which both hydroxy groups occur on one face (Fig. 3), is consistent with the geometry of the active site substrate-binding cavity with respect to the di-iron active site oxidant (Lindqvist et al., 1996), in which stearate binds in a quasi-eclipsed conformation at C9 and C10, projecting the pro-(R) hydrogens toward the active site oxidant (Behrouzian et al., 2002). Future availability of a crystal structure of the T117R mutant in complex with bound oleoyl-ACP, or of the T117R mutant alone or with substrate bound as previously modeled (Whittle et al., 2008), would be useful starting points for probing mechanistic models using computational methods such as density functional theory. Indeed, homology modeling was recently shown to be a useful approach for elucidating selectivity mechanisms of desaturase enzymes such as FAD2 and FAD3 (Cai et al., 2018).
The low levels of 9,10-dihydroxystearate in castor suggests that this system is not optimized to produce this particular product. Higher levels of the diol may accumulate via enzymes with active site geometries that permit more efficient dioxygenation. Cardamine impatiens is an example of a plant that accumulates ∼25% of 9,10-dihydroxystearate (and its chain-elongation products) in its seed oil (Mikolajczak et al., 1964). It is tempting to speculate that it contains a desaturase that has undergone mutation/selection to optimize the production of the diol from the initial alkene product. Examples of desaturases with multiple sequential oxidation activity include English ivy (Hedera helix) that can perform Δ9- followed by Δ4 desaturation on stearoyl-ACP (Guy et al., 2007); FM1, a fungal membrane desaturases that sequentially inserts a Δ12 followed by a Δ15 double bond into oleoyl-phosphatidyl ethanolamine (Cai et al., 2018); and an insect multifunctional enzyme that functions as a Δ11 desaturase, Δ11 acetylenase, and Δ13 desaturase (Serra et al., 2007).
Major oxygenated fatty acids such as ricinoleic- and vernolic acids are typically produced in the endoplasmic reticulum by variant FAD2 membrane-bound desaturases (van de Loo et al., 1995; Lee et al., 1998). On the other hand, fatty acids with unusual double bond positions such as 16:1Δ4, 16:1Δ9, and 18:1Δ6 are synthesized within the plastid (Shanklin and Cahoon, 1998). Thus, the production of oxygenated fatty acids such as the erythro-9,10-dihydroxystearate in the plastid as reported here is very unusual if not unique. It is likely that in species with high levels of accumulation such as C. impatiens, there exists a variant acyl-ACP thioesterase that cleaves the vicinal diol fatty acid from its ACP adduct in addition to specialized acyltransferases and other components that facilitate its transfer from the plastid to triglyceride storage lipids.
More than 70 years ago, 9,10-dihydroxystearate was reported as a component of castor oil (King, 1942) at ∼1% of the total fatty acids (Sreenivasan et al., 1956). The stereochemistry of the diol was later determined to be the erythro configuration (Morris and Crouchman, 1972). Consistent with these earlier reports, castor oil samples evaluated in this work contained ∼0.7% of erythro-9,10-dihydroxystearate. That the wild-type castor desaturase can produce this compound was an entirely unanticipated result and resolves a long-standing mystery. In addition, our results underscore the remarkable plasticity of the nonheme di-iron catalytic center found in the desaturase family of enzymes. It appears that subtle changes in the active site architecture found in these versatile oxidants can allow new reaction pathways to emerge. Further detailed mechanistic work is needed to understand the relationship between reaction outcome and details of the active site architecture.
Mutant Construction
Synthesis of the castor (Ricinus communis) desaturase triple mutant T117R/G188L/D280K and D280K single mutants were as described in Whittle et al. (2008) and Guy et al. (2011). The single mutants T117R and G188L were identified by mutagenesis-selection experiments (Whittle and Shanklin, 2001). The open reading frames were introduced into pET9d using XbaI and EcoRI restriction sites and the resulting clones were validated by sequencing.
Mutant Analysis
Desaturases, and variants thereof, were overexpressed in Escherichia coli BL21(DE3) with the use of pET9d. Recombinant desaturase was enriched to >90% purity by 20CM cation exchange chromatography (Applied Biosystems). Desaturation reactions (600 μL; Cahoon and Shanklin, 2000) were performed by incubation of the desaturase with 18:0- and 18:1-ACP substrates in the presence of recombinant spinach ACP-I (Beremand et al., 1987). Uniformly deuterated stearate was obtained from Cambridge Isotope Laboratories and 9,10 d2 oleate and 11,11 d2 oleate was obtained from the collection of Tulloch (1983). Experiments reported herein were replicated three or more times and representative results are presented.
Fatty Acid Analysis
Fatty acid methyl esters (FAMEs) were prepared by addition of 2 mL of 1% (v/v) NaOCH3 in methanol and incubated for 60 min at 50°C. Fatty acid methyl esters were extracted twice into 2 mL of hexane after acidification with 100 μL of glacial acetic acid. Hexane was evaporated to dryness under a stream of N2, and samples were resuspended in hexane for GC analysis. FAMEs were dried and resuspended in 100 μL of n,o-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and trimethylchlorosilane (TMCS; Supelco) for 45 min at 60°C to create trimethyl silyl derivatives. Samples were analyzed with an HP5890 gas chromatograph (Agilent) fitted with a 60-m × 250-µm SP-2340 capillary column (Supelco). The oven temperature was raised from 100°C to 160°C at a rate of 25°C min−1 and from 160°C to 240°C at a rate of 10°C min−1 with a flow rate of 1.1 mL min−1. Mass spectra were analyzed using an HP5973 mass selective detector (Agilent). For 18O experiments, oxygen was removed from the sample cell by repeated evacuation and purging of the cell with O2-free argon using a Schlenk line. Two mixtures were prepared—one containing desaturase enzyme, buffer, ferredoxin NADPH+ reductase, and substrate, and the other containing ferredoxin and NADPH. The two anaerobic mixtures were transferred to sealed reaction vials containing an atmosphere composed of either 16O2, 18O2 (Cambridge Isotope Laboratories), or an equimolar mixture of 16O2 and 18O2. Reactions were terminated by the addition of toluene, and fatty acids were esterified and silylated as described above for experiments designed to fragment the fatty acid to reveal the position of the vicinal hydroxyl groups. Alternatively, for the labeled oxygen experiments designed to determine the reaction mechanism, fatty acids were converted to methyl esters after which vicinal hydroxy groups were converted to their acetonide derivatives (Singh et al., 2008). To achieve this, methyl ester samples were dried under nitrogen and resuspended in 40 μL of 4 mm of ZrCl4 catalyst in diethyl ether, 200 μL of dichloromethane (CH2Cl2), and 5 μL of dimethoxypropane. The mixture was incubated with shaking at 22°C for 2 h The mixture was extracted with 3 mL of chloroform (CHCl3) and 1 mL of water, separated by centrifugation (at 1,500g for 5 min) and the lower phase was collected and dried under nitrogen before resuspension in hexane for GC–MS analysis. Samples were analyzed on an HP6890/5973 GC–MS equipped with a 30-m × 250-μm HP 5MS capillary column (Supelco). Oven temperature was held at 100°C for 2 min, raised to 300°C at the rate of 20°C min−1, and held for 2 min.
Accession Numbers
Sequence data from this article can be found in the GenBank/EMBL data libraries under accession number M59857.
We thank Dr. John Lipscomb, Dr. Lawrence Que, Dr. Brian Fox, Dr. Diane Cabelli, and Dr. Xio-Hong Yu for helpful discussion and Dr. Pat Covello for providing some of the deuterated compounds prepared by Tulloch (1983).
↵1 This work was supported by the United States Department of Energy, Office of Science, Office of Basic Energy Sciences (contract no. DE-SC0012704 to J.S.).
↵[OPEN] Articles can be viewed without a subscription.
- Received September 10, 2019.
- Accepted November 20, 2019.
- Published December 5, 2019.


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The Nerdy Point of View Podcast is hosted by Brian Penaloza, Kerry Lyons, and Martin Franklin. A weekly podcast where the hosts discuss random topics for your entertainment and explore the nerdier side of life. Available on your favorite Podcast App.
Designed by Matty @SoMattyGameZ
Losing your keys is both scary and frustrating. Keep them organized while making them easily identifiable with this unique keychain. This versatile double-sided keychain is made of heavy-duty metal with a shiny chrome finish which gives it a very sharp & polished look.
• Size: 1.25" x 3"
• Double-Sided Print
• Metal Construction
• Chrome Finish
• Clear Acrylic Insert Protector


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Like any major holiday, Easter comes with its own food traditions — and the chance to tweak those traditions ever so slightly to create something new.
In my family, Easter, which is this Sunday, usually meant a big ham, and trimmings like scalloped potatoes and warm applesauce.
This year, I wanted to mix it up with a different protein. I turned to lamb, a very traditional Easter main course that came from the Jewish food traditions practiced during Passover.
But cooking lamb at home can be difficult if you're not used to it. Do you go with a whole roast? A rack of chops? Chops are the most luxurious cut of lamb, often the most expensive but super tender when cooked properly. You could marinate a lamb leg and throw it on the grill, the lean part of the animal offering nice steaks. Ground lamb makes for sturdy meatballs, especially when mixed with another meat like beef or turkey.
A roast may be the most straightforward option. Try the shoulder, a marbled cut best purchased boneless that is perfect for roasting. Season it with dried herbs like rosemary, salt and pepper, then let it cook for a couple of hours.
I went with something slightly different, equally appealing to those in my family who love lamb and those who merely tolerate it: stew!
Lamb is a natural choice for a stew with Moroccan flavors like coriander, fennel and cumin. It cooks much like beef in this form, imparting a slightly gamier flavor that gives this dish some personality.
You could definitely use beef instead, if you want.
I recommend serving this stew with some pita bread or na'an, and a big, fresh salad made up of lettuce and lots of fresh herbs. Mint goes beautifully with lamb dishes. Throw some citrus in there, too.
This is a good anytime stew, but it's especially nice for a celebratory family meal, the warm spices and hearty lamb putting a twist on the standard holiday roast. And cleanup is easier, too.
Moroccan Lamb Stew
1 tablespoon ground cumin
2 teaspoons ground coriander
1 ½ teaspoons salt
1 teaspoon fennel seeds
½ teaspoon cayenne pepper
½ teaspoon ground black pepper
2 ½ pounds trimmed boned lamb shoulder, cut into 1 ½- to 2-inch pieces (you can also use stew beef)
4 tablespoons olive oil, divided
1 large onion, finely chopped
2 large carrots, cut into ¼-inch-thick rounds
1 tablespoon tomato paste
1 (28-ounce) can whole, peeled tomatoes
2 cups low-salt chicken broth
1 (15 ½-ounce) can chickpeas, drained
A pinch of cinnamon
2 teaspoons grated lemon peel
2 tablespoons chopped fresh mint, for serving
Greek yogurt, for serving
Mix first six ingredients in a large bowl. Add lamb and toss to coat. Heat 2 tablespoons oil in heavy large pot over medium-high heat. Add lamb to pot and cook until browned on all sides, turning occasionally and adding more oil if needed to keep the pot from drying out.
Add onion, carrots and tomato paste. Reduce heat to medium; saute until onion is soft, about 5 minutes. Add tomatoes, broth, chickpeas, cinnamon and lemon peel and bring to a boil, scraping up browned bits. Bring to a boil.
Reduce heat to low, cover and simmer until lamb is just tender, about 1 hour. Uncover and simmer until sauce thickens enough to coat spoon, about 20 minutes. Use a spoon to break up whole tomatoes a bit. Taste stew and season with salt and pepper as needed.
Transfer to serving bowls, sprinkle with just a bit of mint and top with a dollop of yogurt.
Serves about 6.
Source: Adapted from Epicurious


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The Story: Don't eat the fruit in the garden, Eden,, It wasn't in God's natural plan., You were only a rib,, And look at what you did,, To Adam, the father of Man.
The Story: You smell like goat, I'll see you in hell
The Story: All the b***h had said, all been washed in black
The Story: And my blunt is my ho now
The Story: At home, drawing pictures, Of mounds of tots, With ham on top
I just looked up the song because I thought, What the hell is "yubby"? and I thought maybe the juke of crush was a dance.
Submitted by
Submitted on
11/29/2012 12:41 pm
Age it happened
Better than original?
Convinced others you were right?
Was partying involved?
Have a comment? Log in for free today so you can post it!
I'm not sure, maybe alot of bumping and grinding and...crushing. sounds painful! haha!
steph24 Jan 14, 2013 11:12 am
That's too funny. I wonder how you would do the juke of crush if it really was a dance.
Seikatsu5 Jan 13, 2013 4:52 pm


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Deputy PM supports issuance of Hanoi Declaration at transport forum
Deputy Prime Minister Trinh Dinh Dung on Monday expressed his support for the issuance of the Hanoi Declaration at the 12th Intergovernmental Regional Environmentally Sustainable Transport (EST) Forum in Asia that is underway in Hanoi until Wednesday.
Deputy PM Dung said gaps in the development of transportation infrastructure and urban infrastructure hinder Vietnam's sustainable development. He said he hopes the participants will discuss ways to improve socio-economic and environmental efficiency to boost smart transportation solutions by integrating an effective traffic management system and public-private partnerships. Mr. Dung said: "Asia is home to large economies and has great potential to develop policies and investment in transportation. The forum allows policy makers and sponsors to discuss ways to enhance a sustainable transportation infrastructure system. I support the issuance of the Hanoi Declaration to realize smart cities in Asia."
The forum includes sessions on policies for a sustainable transportation infrastructure system, decreasing air pollution in urban areas, and upgrading transportation services and infrastructure. Participants also discussed ways Asia can contribute to the 2030 UN Agenda for Sustainable Development.
Source: VOV5


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*Packaging may vary
North Peak Wanderer Session IPA
Session IPA /4.2% ABV / Michigan, United States
- Category
- Session IPA
- Region
- Michigan, United States
- Type
- Craft
- 4.2%
- Suggested Glassware
- Pint Glass
- Suggested Serving Temperature
Product description
North Peak Wanderer is a Session IPA. This exciting new style is balanced with malt and hop characters and has a clean finish. The combination of full hop flavor and lower alcohol gives Wanderer a a wonderful drinkability! Wanderer has a light, clean and refreshing body, and is hopped with Perle, Willamette, Centennial and Citra hops that gives Wanderer a Citrus and Pine finish. Wanderer is rounded out with a generous amount of dry-hopping with Citra hops to fill the nose. It is brewed in Traverse City, MI, on Old Mission Peninsula incorporating locally grown hops.View all products by North PeakCalifornia Residents: Click here for Proposition 65 WARNING


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You can find a lot of important and intereseting product information on our website:
- Download of installation manuals by using our search function (search for the order id of your E-Kit) or selecting your vehicle
- Detailied product information about new and innovations by using our Newsletter or News. Our News-Area is also available as a RSS-Feed
- Downloads and information about a lot of our products
- Detailed definitions in our Glossary, which is continuously developed by us
Steadily growing collection of frequently asked questions and answers in our FAQ
Quick selection:
- Installation manuals by using our vehicle selection or search
- Newsletter
- News
- Downloads
- Glossary
We are at your service!
If you do not find the needed information on our website, please feel free to contact us. The easiest way to get in contact with our company is by writing us an e-mail firstname.lastname@example.com
If you have an questions about installation of our E-Kits, please feel free to contact us vie email email@example.com or phone.
You can call our Helpline via Tel: +49 (0) 6031 - 79 4444. There you can reach a lot of qualified employees who will help you with all their knowledge.


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Big Pimpin'
What do Ariane's space rockets, Porsche's racing cars, Liebherr's largest industrial cranes and some of the world's most technically advanced suspension bridges have in common with Ultime Trimarans, Maxi 72s and most of the recent contenders in the America's Cup? They all rely on cutting-edge composite solutions from Carbo-link to solve extremely complex structural engineering challenges and gain a competitive advantage.
Carbo-Link's roots are in hi-tech civil and industrial engineering – the company is a spin-off from the highly regarded Swiss Federal Laboratories for Materials Science and Technology (EMPA) – but while it remains a world leader in these fields, working on large-scale infrastructure projects, its technology has migrated into other industries including aerospace, motor sport and high-performance sailing. Since the turn of the millennium, a major focus for Carbo-Link's innovation has been the development and refinement of new, often groundbreaking, rigging solutions for high-performance racing and superyachts. Read on.
top shelf
Big Pimpin'


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A giftset is a package in which multiple Transformers toys (usually, members of the same combiner team) are sold together. Generally, the term "giftset" is not used to refer to multi-packs of toys from the smallest size classes, such as Micromasters or Mini-Cons.
Giftsets by country
Japan was the only country where the G1 Predacons were sold as a giftset, while the Combaticon and Terrorcon giftsets were only available in Japan and Italy. Additionally, Italy was also the only place where Nautilator was actually included in the Seacon giftset.
In Greece, El Greco's locally manufactured versions of the Constructicons and Protectobots were sold exclusively in giftsets.
In Japan, Sunstreaker, Skids and Buzzsaw were only available in the VS-Z giftset.
You left a piece out!
This article is a stub and is missing information. You can help Teletraan I: The Transformers Wiki by expanding it.


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Kittens can grow very large
Werewolves, Vampires and Demons, Oh My #3
Eve Langlais
(15 April 2018)
I'm a witch. Not a good one or a bad one, although according to the snooty wizards, I'm a stain upon their robes.
But who cares what those snobs say?
So what do you do if your newly rescued kitten gets out and doesn't come home? Especially if there is a good chance its body has been taken over by a demon? Why tickle it under the chin and hug it of course!
Yes its demons versus witches – and sorcerers – and all sorcerers are male misogynists – or are they?
A feisty witch (human and not having magic blood – or so the sorcerers claim) sets out to show the males just how demons should be fought. And how you can love some, and they will be faithful to you. See kitty!
Great fun story. Lots of humour and snark. And yes, the males get shown up by the females!


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About This Listing
The kit is finished in Tobacco Burst Gloss Lacquer FinishPlyTM. As is the case with all Design SeriesTMåÊdrums, the shells consist solely of North American maple with no reinforcement hoops.
FREQUENT FLYERTM: GO PLACES Peter Erskine approached the DW design team with a novel question,"What if there was a travel kit that didn't sound like a travel kit?" Seems obvious enough, but the minds at DW had never tackled the quandary head-on, as it wasn't a simple task. So they went to work on a kit that was just small enough to be travel-friendly, but also substantial enough to sound like a 'real' drum set should. After, literally, years of R&D and discussion, the sizes were agreed upon: a 12x20" bass drum with low-mass spurs and lightweight ratchet bass drum mount, an 11x14" floor tom with aluminum legs, a traditional 8x12" rack tom with STM, and a matching 5x14" snare drum. The kit also comes standard with True-PitchTMåÊtension rods, MAGTMåÊthrow-off, True-ToneTMåÊsnare wires, and DW Heads by Remoå¨.
- 8x12" Rack Tom
- 11x14" Floor Tom
- 12x20" Bass Drum
- 5x14" Snare Drum
Product Specs
|Brand New (New)
Brand New items are sold by an authorized dealer or original builder and include all original packaging.learn more


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Jason stared out through the cold glass porthole of his containment module, confused and disorientated, feeling as if he had just woken up from a nightmare.
"Where am I?" he wondered, his lethargic and foggy mind racing to make sense of his surroundings.
"Hello!" he tried to call out loudly hoping that someone would come to his aide, but the words refused to take form adding to his sensation of panic and fear.
"I can't feel my body!" he suddenly realised with alarm while attempting to look around, the panic now giving way to sheer terror.
"Think Jason, think!"
He scolded himself for allowing his fear to cloud his rational mind. He was a scientist after all, one of the very best in his field who prided himself on his ability to reason things out.
He drew from his deep reserves of sheer willpower calming himself down, but he felt so tired and sleepy from the effort. Exhaustion set in and he felt himself slowly slipping away into blissful slumber, succumbing to the encroaching darkness.
The Equinox sailed along placidly in geostationary orbit precisely 35900 kilometres above the Earth's equator, her position matching that of the Earth Space Agency's headquarters far down below, her name proudly emblazoned in red letters on both sides of her reinforced hull.
She was a massive ship by any comparison, almost two kilometres in length and close to six hundred metres wide, and a real work of art from an engineering perspective.
This deep space exploration and mining vessel, the largest ever built, was the epitome of mankind's progress and ingenuity when she first sailed out of space dock fifteen years ago, ready to explore the solar system and haul back a fortune in valuable metals, a commodity long exhausted on Earth from centuries of mining.
But that was fifteen years ago.
The Equinox was now a prison ship, home to those who were sentenced to life imprisonment for crimes against the tyrannical and all powerful United Earth Government. The hapless prisoners, many of whom had spoken out against the regime, were removed from society, suspended, frozen and locked away forever, never to be heard from again.
Jason eventually drifted back into consciousness from a dreamless sleep. He had no idea how much of time had passed since he was last awake but at least he was much calmer this time around and his mind was focussed. He was ready to deal with his situation rationally.
His vision was still quite distorted and hazy as if he had on very strong prescription spectacles, but it was enough for him to be able to see the row of modules directly across the room causing a glimmer of realisation to surface.
"Those are suspension modules!"
The realisation brought his memory flooding back in an instant. He was a prisoner, sentenced to life imprisonment aboard the Equinox!
The sensation of panic which he was starting to feel again quickly gave way to curiosity.
"I'm frozen, so logically, I shouldn't even be conscious," he marvelled. "How is this even possible?"
He focussed his attention on the view in front of him while trying to supplement his restricted visibility with his memory of the room from when he was first brought in and placed in his containment module.
The little square metallic room housed fifteen steel containers which resembled coffins standing upright. A multitude of pipes and cables emerged from them and snaked off towards the ceiling.
Cold, icy, frozen faces with sightless blank eyes stared out the glass portholes at the front of each module, some appearing calm with closed eyes while other faces held expressions of terror permanently etched onto them.
Directly below each glass porthole was a little touch screen indicating the health status of the occupant. The mostly red and green light emanating from the little screens illuminated the lifeless looking faces of the occupants with an eerie glow.
The steel containers were arranged against three of the four walls, five per wall, with the forth wall housing the door and various mounted controls and instruments showing off dials and flashing lights.
Thinking back to the day he was imprisoned, Jason remembered that his colleague and friend, Vince, occupied the module next to his.
"It's all my fault that he's in there," thought Jason remorsefully as he drifted into blissful slumber once more.
Meanwhile, beyond the reinforced hull, far down below on the planet's surface, a new dawn was breaking. The Earth appeared to be a serene mix of dark blue oceans and wispy white clouds contrasted with distinct land masses. The major cities and population centres were bright enough to be seen from this altitude, illuminated by millions of light bulbs. It was truly a breathtaking sight, if anyone had bothered to notice.
Jason woke up yet again from another dreamless sleep. His perception of time had improved somewhat since the first time he had awakened inside his module.
This was all still a rather strange and disconcerting experience for him, being fully aware yet completely paralysed. After three weeks of enduring this he doubted that he would ever get used to it. His moments of wakefulness had grown significantly longer now giving him plenty of time to marvel at the helplessness of his situation.
Today Jason thought about his parents back on Earth. Feelings of sadness and nostalgia overtook him. He thought about how devastated they had been when he and his friend Vince were arrested. They stood by him and had been supportive throughout the short and decisive criminal trial which followed even though the end result was a foregone conclusion. He had drawn on their strength to endure the process.
"I wonder if everyone else here is also experiencing consciousness during suspension?" he wondered to himself, his thoughts going off on a tangent, "Or am I an isolated case?"
"I hope mum and dad are ok."
A movement in his field of vision interrupted his thoughts quickly bringing him back into the present. It was the guard on his rounds again, checking on the status of every module and its prisoner.
Jason watched as the guard walked towards his module and examined the little screen below his porthole. The guard was close enough for Jason to read the name imprinted on the tag which was neatly pinned onto the flap of his shirt pocket. It read Salves.
"Let me out of here!" screamed Jason in his mind.
It was a thought born out of the sheer frustration and the helplessness he felt from being immobile and fully conscious for so long.
The guard suddenly turned around startled, as if he had heard a sound. A look of surprise and shock crossed his face while his eyes fearfully searched the room for the source of the disturbance. Finding no rational explanation he hastily fled the room.


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Why Are Roller Shades In Edmonds Selected By So Many People For Their Windows?
Are you looking into the different types of window treatments to help you find the right one for your windows, so you can get them all covered? Have you noticed that roller shades in Edmonds seems to be one that is selected by a lot of people for the windows of their home, and you are wondering why? There are multiple reasons why these window coverings are chosen by so many people everywhere.
Once you have been informed about these reasons, you will not hesitate to get them up on all the windows of your house as well. Below are the reasons that are the most imperative for you to learn about right now.
One: Light control – Many people like to choose how much light gets into each room of their house. With many types of window coverings this is not possible, but with these window shades it is easy to control exactly how much light gets into every room. There are different opacity levels that will provide you with a wide range of light filtering options.
This means that you can easily control the light that gets into every single room of your house by selecting the perfect opacity level for each room.
Two: Excellent UV protection – Another thing that many people like to control is how much UV gets into their home. These window coverings offer different openness factors that range from 1% to 25%, which will help to provide the perfect UV protection in each room.
This will help you to protect the belongings in every room of your house easily. It will also help you control the sun in every room, so you can make sure that each room of the house is comfortable for every family member all the time.
For example, you will be able to keep the sun completely out of the bedrooms when they shades are closed so that everyone can get better sleep.
Three: Put your own personal style on each window – When you decorated your home, you carefully chose the home décor in each room so that you have put your own personal style into each room, and so you really enjoy and are comfortable in every room of your house. You want to do the same thing with the window treatments that you choose for each room, so you enjoy the look of every window as well.
There are more than 240 decorative fabric choices with different patterns, colors and other options to choose from. This helps you easily find what you like for each window and that fits well with the home décor you have chosen for each room. Just make sure that you choose the window coverings that complements the home décor as well.
Now that you know the reasons why roller shades in Edmonds are selected by so many people everywhere for the windows of their home, you can understand why these are the smart choice for every window in your house as well. Make the smart decision and get them up on each window now so you can begin enjoying all that they offer as quickly as possible in your own home.


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Early 50s Kodak slides of a modernist home under constructio
Posted: Tue Mar 25, 2008 10:26 am
Recently, I was sent a CD full of digitized Kodak slides from the early 1950s, showing a Wrightian home during, and shortly after, construction. While time has taken its toll on some of these images, I thought a few members might like to see the vehicles, tools, construction methods, and day-to-day artifacts and attire of an earlier time. In some of the views, the Redwood looks so fresh one can almost smell it.
The slides can be seen .
The slides can be seen .


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Back to the list of resources in Civ4
Pigs provide food and health to the city that works them.
Civilopedia entryEdit
The pig has been domesticated for about 5,000 to 7,000 years and the animal is found across Europe, the Middle East, and extends in Asia as far as Indonesia and Japan. Pigs are one of the most intelligent animals, and some are even kept as pets. Pigs are reportedly more trainable than dogs and cats.
Community content is available under CC-BY-SA unless otherwise noted.


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As a special New Years gift to its loving fans Arsenal.com is offering a FREE video on the Arsenal Player- the Arsenal Insider series starring Johan Djourou
Each week Johan presents his personal diary from the training ground. It's a chance to get a player's view of recent games and all the big talking points. The Swiss centre back holds court in the boot room and beyond, taking you behind the scenes at London Colney.
http://www.arsenal.com/news/news-archiv ... al-insider
The (not so) secret diary of Johann Djourou aged 24 3/4
As we're unlikely to see terraces again at football, this is the virtual equivalent where you can chat to your hearts content about all football matters and, obviously, Arsenal in particular. This forum encourages all Gooners to visit and contribute so please keep it respectful, clean and topical.
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Keywords: OroCRM - Windows - How to - Email configuration (SMTP)Description: How to configure OROCRM Ver 3.0.0-1 Windows Localhost Gmail SMTP?
Hi @jeevarathinam,
You can find how to configure SMTP for the Bitnami OroCRM Windows installer in this link to our documentation.
Best regards,Alejandro
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