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Genome-wide antisense transcription drives mRNA processing in bacteria
Edited by Susan Gottesman, National Cancer Institute, Bethesda, MD, and approved November 8, 2011 (received for review August 19, 2011)
RNA deep sequencing technologies are revealing unexpected levels of complexity in bacterial transcriptomes with the discovery of abundant noncoding RNAs, antisense RNAs, long 5′ and 3′ untranslated regions, and alternative operon structures. Here, by applying deep RNA sequencing to both the long and short RNA fractions (<50 nucleotides) obtained from the major human pathogen Staphylococcus aureus, we have detected a collection of short RNAs that is generated genome-wide through the digestion of overlapping sense/antisense transcripts by RNase III endoribonuclease. At least 75% of sense RNAs from annotated genes are subject to this mechanism of antisense processing. Removal of RNase III activity reduces the amount of short RNAs and is accompanied by the accumulation of discrete antisense transcripts. These results suggest the production of pervasive but hidden antisense transcription used to process sense transcripts by means of creating double-stranded substrates. This process of RNase III-mediated digestion of overlapping transcripts can be observed in several evolutionarily diverse Gram-positive bacteria and is capable of providing a unique genome-wide posttranscriptional mechanism to adjust mRNA levels.
For many years, the catalog of transcripts (transcriptome) produced by bacterial cells was limited to the transcription products of known annotated genes (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA). In the past 10 years, the development of new approaches based on high-resolution tiling arrays and RNA deep sequencing (RNA-seq) has uncovered that a significant proportion (depending on the study, varying between 3% and >50%) of protein coding genes are also transcribed from the reverse complementary strand (1–17). In most cases, overlapping transcription generates a noncoding antisense transcript whose size can vary between various tens of nucleotides (cis-encoded small RNAs) to thousands of nucleotides (antisense RNAs). The antisense transcript can cover the 5′ end, 3′ end, middle, entire gene, or even various contiguous genes. Alternatively, overlapping transcription can also be due to the overlap between long 5′ or 3′ UTRs of mRNAs transcribed in the opposite direction. Independent of the mechanism by which it is generated, overlapping transcription has been proposed to affect the expression of the target gene at different levels [for review, see Thomason and Storz (18)]. These mechanisms include: (i) the overlapped transcript affects the stability of the target RNA by either promoting (RNA degradation) or blocking (RNA stabilization) cleavage by endoribonucleases or exoribonucleases; (ii) the overlapped transcript induces a change in the structure of the mRNA that affects transcription termination (transcription attenuation); (iii) the overlapped transcript prevents RNA polymerase from binding or extending the transcript encoded in the opposite strand (transcription interference); and (iv) the overlapping transcript affects protein synthesis either blocking or promoting ribosome binding (translational regulation). Although all these regulatory mechanisms have been proposed based on studies with specific sense–antisense partners, the presence of massive amounts of overlapping transcription strongly suggest that it might serve for a general purpose on bacterial gene expression (5, 18–24).
In this work, we used RNA sequencing to analyze both the long and short RNA fractions of the major human pathogen Staphylococcus aureus. S. aureus is a common asymptomatic colonizer of the skin, nasopharynx, and other mucosal surfaces of approximately one-fourth of the healthy human population. However, when S. aureus traverses the epithelial barrier, it becomes a leading cause of many diverse pathological syndromes, such as abscesses, bacteremia, endocarditis, osteomyelitis, and pneumonia (25). S. aureus has emerged as a model organism for the study of bacterial regulatory RNAs because key discoveries in bacterial regulatory RNAs have been achieved in this bacterium. In 1993, Novick and coworkers (26) identified the first example of a regulatory RNA (RNAIII) that controls the expression of virulence factors by pairing with the target mRNAs followed by degradation of the RNAIII–mRNA complex by the double-stranded specific RNase III (27). More recently, several studies using computational analysis of intergenic regions, microarray technology, and deep sequencing have allowed the identification of >140 small RNAs, including both trans- and cis-encoded antisense RNAs (10, 28–32). In this current study, we uncover the existence of an overlapping transcription process covering, in a genome-wide extent, the expressed protein coding genes. Base pairing between overlapping RNAs can create double-stranded substrates for RNase III endoribonuclease activity. Such duplex regions promote the cleavage of the double-stranded RNA and the generation of short RNAs (average size of 20 nt). Thus, a collection of stable small RNA molecules that symmetrically map both strands of every region with overlapping transcription is generated. The presence of an identical collection of short RNA molecules that symmetrically mapped both strands of annotated ORFs in Enterococcus faecalis, Listeria monocytogenes, and Bacillus subtilis indicated that this process is evolutionary conserved in Gram-positive bacteria.
Pervasive Antisense Transcription in S. aureus.
A systematic and hierarchical strategy (Fig. S1) to characterize both long and short RNA (<50 nt) fractions from log-phase growing S. aureus cells was developed. Long RNA sequencing was performed by using a cDNA synthesis procedure that preserves information about a transcript's direction based on the incorporation of deoxy-UTP during the second strand synthesis and subsequent destruction of the uridine-containing strand (33). The resulting 76-bp paired-end reads were mapped to the S. aureus NCTC 8325 reference genome. A total of 9.7 million uniquely mapped read pairs were identified (Fig. S1). 49.2% of the genome was covered by uniquely mapped reads on both strands, 40.4% by uniquely mapped reads on one of the strands, and 10.4% showed no coverage (Fig. 1A). Of the 2,653 annotated ORFs of the S. aureus genome, which covers ∼84% of the genome, we detected expression of 2,181 ORFs (coverage of >90%), of which 1,387 ORFs displayed 50% coverage on the antisense strand (Fig. 1B).
Naturally occurring short RNAs were also sequenced in a strand-aware fashion by using a two-step adaptor ligation procedure to the 3′ and 5′ ends of the RNA molecules (34). The reads were aligned by algorithmically clipping off the 3′ adapter, and the remaining sequences of each read were mapped to the genome by using STAR (http://gingeraslab.cshl.edu/STAR/). For alignments of 10–19 bases long, up to one mismatch was allowed; for alignments >20 bases, up to two mismatches were allowed. Alignments of <10 bases were discarded, and spliced alignments were prohibited. This process yielded a total of 7,778,726 million reads mapped to the genome (Fig. S1). The average length of short RNA molecules was 20 nt (Fig. S1). The uniquely mapped short RNA sequences covered, in at least 50% of their length, 2,268 and 1,981 ORF regions on the sense and antisense strands, respectively (Fig. 1C). Thus, the percentage of ORFs covered in at least 50% of their length by reads in the antisense strand was higher in the case of short RNA (75%) than in the case of long RNA (56%), suggesting that short RNA libraries may prove to be a more sensitive way to detect antisense transcripts. Overall, and given that long and short RNA libraries were generated independently—that is to say from two fractions coming from the same RNA sample—these results provide evidence of the existence of antisense transcription not seen in the long RNA sequence analysis.
Symmetric Distribution of Short RNA Reads in both Strands of ORF Regions.
We next sought to determine whether the distribution of short and long reads for a given ORF were linked. For that, we visualized normalized log2 values representing the number of mapped reads per nucleotide using the Integrated Genome Browser (IGB) (35). Fig. 2 illustrates a randomly selected 30-kb region of the genome of S. aureus that represents 1% of the genome and depicts the uniquely mapped long and short RNAs. The results revealed that short RNA sequences were symmetrically distributed in both strands of the ORFs, whereas long RNA transcripts follow the expected biased distribution toward the sense strand. Intriguingly, the regions with detectable overlapped transcription between long RNA transcripts, such as those regions corresponding to antisense transcripts to ORFs (00056, 00061, sirABC operon), were covered with higher numbers of short RNA reads in both strands. Similar symmetrical accumulation of high levels of short RNAs was detected in every region of the genome where noticeable overlapping transcription occurs, such as 5′ and 3′ overlapping UTRs, overlapping operons (ORFs that, being located in the middle of an operon, are transcribed in opposite direction to the other genes of the operon), and antisense transcripts (see Fig. 3 and Figs. S2–S4 for additional examples). To most accurately demonstrate that the distribution of short RNA reads was symmetric genome-wide, we quantified the number of long and short RNAs mapping to the sense and antisense strands of each ORF. In accordance with the images observed with the IGB browser, the results revealed very similar numbers of short RNA reads genome-wide in both strands of ORF regions and the expected biased distribution of long RNA reads in the sense strand (Fig. 4 A and B). In summary, these results show that the S. aureus transcriptome contains both long and very short RNA molecules. The amount of long RNAs is, as expected, higher in the sense strand of each ORF. In contrast, short RNAs are equally distributed in both strands of each ORF and specially enriched in those regions with detectable overlapped transcription between long RNAs.
RNase III Is Responsible for the Production of Symmetrically Distributed Short RNA Populations.
The fact that short RNAs display a symmetrical distribution in sense/antisense strands and accumulate in higher numbers in regions with noticeable overlapping transcription raised the possibility that short RNA molecules were derived from the cleavage of overlapping long sense/antisense primary transcripts. S. aureus genome has been reported to encode at least eight putative endoribonucleases and three exoribonucleases (32). Among them, the RNase III endoribonuclease is the only enzyme known to be able to degrade double-stranded RNA. Thus, we tested the possibility that RNase III might be responsible for processing the overlapping transcripts into symmetrically distributed sense and antisense short RNA populations. An RNase III mutant in the S. aureus 15981 background (S. aureus 15981 Δrnc) was constructed by using a described approach (36).
Analysis of the uniquely mapped reads from long RNA-seq of the RNase III mutant revealed that the percentage of the genome covered by reads on both strands increased up to 74.2% compared with wild-type strain (49.2%; Fig. 1A). This increase was mainly due to a significantly higher coverage of the antisense strand (82% ORFs displayed 50% coverage on the antisense strand; Fig. 1B). In contrast, the number of the short RNA reads was drastically reduced, especially in the antisense strand (Fig. 1C), reducing the percentage of the genome that was covered on both strands by short RNAs to only 6%. Of note, the median length of the short RNA molecules in RNase III mutant was 15 nt, suggesting the possibility that short RNAs detected in the RNase III mutant were produced by another RNA processing pathway (Fig. S1). Visualization of the distribution of mapped reads by using IGB confirmed that short RNA sequences had lost their symmetric distribution, whereas long RNA transcripts followed the expected biased distribution toward the sense strand (Fig. 2 and Figs. S2–S4). Accordingly, the correlation first observed in the wild-type strain between the numbers of short RNA reads in sense and antisense strands per annotated ORF region disappeared in the analysis of the RNase III mutant (Fig. 4 C and D). Together, these results indicate that a large majority of short RNA molecules present in the transcriptome of S. aureus are produced by the cleavage activity of double-stranded RNase III enzyme.
Because the pattern and cleavage frequency by RNase III is unknown, the short RNA molecules might be direct products of RNase III activity or processed products of larger RNA fragments generated by RNase III. Pnp is the most important 3′–5′ exoribonuclease activity in bacteria. S. aureus contains a gene (SAOUHSC_01251) encoding a protein that shares 66% identity with Pnp of B. subtilis. We produced libraries from short RNA fraction of S. aureus 15981 Δpnp. Analysis of the mapped reads from S. aureus 15981 Δpnp mutant revealed that the distribution and size of the short RNAs followed a pattern indistinguishable from that of the wild-type strain (Fig. S1), suggesting that Pnp activity is not required for subsequent processing of the short RNA molecules generated by RNase III activity.
Abundance of Short RNAs Correlates with the Levels of Double-Stranded Sense/Antisense Transcripts.
One prediction of the model that short RNAs are produced from the processing of genome-wide overlapping regions of transcription is that the abundance of short RNAs detected should be proportional to the abundance of either the sense/antisense transcripts, depending upon which transcribed strand is less abundant and available for processing. To explore this prediction, we analyzed the short and long RNA complements from a sigma B (sigB) mutant (S. aureus ΔsigB). The transcription factor Sigma B drives the transcription activity of genes under specific environmental conditions. We analyzed ORF regions for which the long antisense transcripts contained a consensus SigB promoter box (Fig. S5), and their expression was significantly suppressed in the sigB mutant (>50% reduction in the ΔsigB/WT antisense transcript ratio based on the long RNA libraries). Consistent with the hypothesis that the abundance of short RNAs depends on the levels of double-stranded RNA, knockdown of the antisense transcripts levels in sigB mutant correlates with a decrease in the amount of short RNAs produced from both strands (Fig. S5). These results indicated that the short RNA abundances at ORF regions are strongly correlated with the less abundant levels of overlapping long RNA capable of forming double-stranded RNA.
Detection of Occurrence and Abundance of Antisense Transcripts in RNase III Mutant.
Detection of antisense transcripts has been difficult in bacteria, and only the presence of a few antisense transcripts has been confirmed by Northern blot techniques. RNAse III cleavage of overlapping RNA transcripts into short RNA molecules could explain at least in part the paucity of antisense transcripts detected so far. Only minimal amounts of antisense transcripts would be maintained in the cell by RNAse III activity. To explore this hypothesis, we performed Northern hybridizations with strand-specific probes to interrogate sense and antisense transcripts of several individual genes in wild-type and RNase III mutant strains. The candidate genes were selected based on their relevance to different aspects of S. aureus virulence (sarA, agrBCDA, saePQRS, clpP) or biology (lexA, recF, yhcSR) (Fig. S6). The results of the Northern analyses indicated a specific absence and presumed degradation of most full-length antisense transcripts in the steady-state condition of the wild-type strain (Fig. 5), whereas the presence of discrete-size antisense transcripts was clearly detectable in the RNase III mutant for all genes tested. It is worth noting that these results confirm the existence of antisense transcripts for genes that have been thoroughly studied because of their impact on S. aureus virulence and antibiotic resistance. For some genes, these hybridizations showed that the RNA levels of the sense strand (lexA, clpP, saePQRS) increased in the RNase III mutant, suggesting that the absence of RNase III cleavage can slightly modulate the expression levels of sense transcripts (Fig. 5). To confirm that the presence of antisense RNA was restricted to those regions where short RNAs were detected, we selected two genes (SAOUHSC_00086 and SAOUHSC_00410) for which very few short RNAs were detectable in the wild-type strain (Fig. S7). In both cases, no transcript antisense to these genes was detectable in the RNase III mutant. Overall, these results uncover the existence of long antisense RNAs transcripts for most ORFs of the S. aureus genome. These long antisense transcripts are underrepresented in the wild-type strain because of the double-stranded RNase activity of RNase III.
Analysis of Short RNA Complement Present in Diverse Bacterial Species.
To investigate whether this genome-wide sense/antisense overlapping transcript processing mechanism is specific to S. aureus or is active in other bacterial species, we characterized the short RNA complement present in three representative Gram-positive (E. faecalis, L. monocytogenes, and B. subtilis) and one Gram-negative (Salmonella enterica serovar Enteritidis) bacteria (Fig. 4E). Short RNA libraries were produced, sequenced, and mapped by using the described protocol (Fig. S1). Analysis of the distribution of short RNAs in sense and antisense strands of ORF regions revealed a highly significant correlation between quantities of short RNAs in sense/antisense strands for the three low-GC content Gram-positive bacteria, mirroring the observations in S. aureus. In contrast, the results obtained from the analysis of Salmonella demonstrated the absence of such a correlation, indicating the existence of a different processing pattern of overlapping RNA pairs in Gram-negative bacteria. Previous transcriptome analysis has allowed the identification of antisense transcripts in L. monocytogenes (5), B. subtilis (17), and E. faecalis (37). Analysis of the distribution of short RNAs in those regions with recognized antisense transcription confirmed the accumulation of high amounts of short RNA in every region with overlapping transcription, indicating that genome-wide digestion of overlapping sense/antisense transcripts is conserved at least in Gram-positive bacteria (Fig. S8).
Development of RNA-seq technology is allowing the characterization of the multiple types of RNA molecules present in a living cell. The application of this technology in bacteria has primarily been restricted to the analysis of long RNA molecules because of the difficulty in removing highly abundant small-sized rRNA and tRNA molecules. Here, we have used two methods developed for microRNA analysis in eukaryotic cells to analyze the RNA fraction of <50 nt of the human pathogen S. aureus. The short RNA fraction was purified by size fractionation electrophoresis, and libraries for RNA-seq were generated by following a protocol that preserves the information about a transcript's direction developed for the direct cloning of microRNA in Drosophila (34).
The analysis of the distribution of short RNA molecules revealed several unexpected results. First, the sense strand of 2,268 ORFs and the antisense strand of 1,981 ORFs were covered with unique short RNA reads in at least 50% of their length, indicating the existence of antisense transcription from both strands of most ORFs in S. aureus genome. Second, similar numbers of short RNAs were mapping to sense and antisense strands of each ORF, irrespective of the transcription levels of the sense strand. Third, short RNA reads accumulated in higher numbers in regions with noticeable overlapping transcription between long RNA transcripts. The simplest interpretation for these observations was that short RNAs were products of the processing activity of a RNase on the double-stranded overlapping RNA transcripts. In support of this explanation, knockout of the rnc gene, which encodes for the only known double-stranded RNase (RNase III) contained in the S. aureus genome, caused a significant decrease in the number of short RNAs, the loss of the symmetric distribution of short RNAs in sense/antisense strands of each ORF, and the accumulation of long RNA molecules (Fig. 1) that, in the case of antisense transcripts, emerge as defined visible bands in Northern hybridizations. Other evidence supporting the hypothesis that short RNA molecules are generated by cleavage of overlapping RNA transcripts was obtained by the analysis of the short and long RNA fractions of the S. aureus ΔsigB mutant. Because the expression of some antisense transcripts requires the presence of the SigB transcription factor, the expression of these antisense transcripts decreases in the sigB mutant strain. The analysis of the distribution of short RNAs for those ORFs in which the expression of the antisense transcript is SigB-dependent revealed a decrease in the number of short RNAs that specifically mapped with the sense and antisense strands of these ORFs indicating that the levels of shorts RNAs is limited by the amount of double-stranded RNA. Notably, in most of these ORF regions, the decrease expression of the antisense RNA correlates with an increase in the expression level of the sense transcript, suggesting that RNase III-dependent sense/antisense cleavage process might serve to modulate the levels of the sense RNA.
Current RNA sequencing techniques needs microgram amounts of total RNA for analysis, which corresponds to millions of bacterial cells. The discovery of the existence of overlapping transcripts in a bacterial population does not mean that sense/antisense transcripts are simultaneously present in the same bacteria. Indeed, the transcriptome map will be identical if a subgroup in the bacterial population will synthesize the sense transcript and another subgroup will synthesize the antisense transcript. Our results imply that overlapping transcription occurs extensively in the same cell because RNase III can only produce short RNAs when both transcripts are present and emphasize that overlapping transcription plays a role in posttranscriptional regulation of RNA.
One question that emerges from these results concerns the biological role of the pervasive overlapping transcription and RNase III-mediated processing. At least two important biological consequences for this process are suggested from these results. First, antisense transcription and RNase III activity could provide a means for the removal of transcriptional noise. In this circumstance, RNase III would digest low-level expression of sense RNA transcription whose expression, if left unchecked, could unnecessarily compete with the processing and translation of required transcripts. When the transcription of the gene is increased in a regulated fashion, the sense RNA levels would exceed that of the antisense expression, leaving the unpaired sense transcripts impervious to RNase III activity and possible productive translation. Such a model predicts that the levels of expression of sense and antisense transcripts would be coordinated to achieve this threshold effect. Second, this mechanism would also permit the fine tuning of the sense transcript levels by adjusting the levels of antisense transcription to levels that allow for more or less final sense transcripts. By controlling which regions within multiple gene operons are subject to overlapping transcription, selection of which genes will be ultimately expressed at the protein level could be regulated. It is worth noting that implementation of this mechanism could be used to avoid simultaneous expression of 5′ or 3′ overlapping transcripts in the same cell.
Another interesting aspect raised by these results is the fate and functional role(s) of the stable short RNAs derived from the processing of overlapping long RNA transcripts. Although the answer to this question clearly requires additional studies, it is important to recall the processing of long RNA precursors into short microRNAs and into short interfering RNA molecules as guided by the eukaryotic RNase III-related ortholog enzymes and consider whether there are similar or related genome-wide regulatory mechanisms ongoing in eukaryotic cells.
Materials and Methods
The strains and oligonucleotides used in this study are listed in Table S1. Methods for bacterial growth, chromosomal gene deletion, RNA extraction, riboprobe synthesis, Northern blot assays, read mapping, and statistics analysis are described in detail in SI Materials and Methods. Short RNA libraries were prepared from an RNA fraction containing RNAs of <50 nt by adapting a described method (34). This fraction was obtained from total RNA with the flashPAGE fractionator (Ambion). Long RNA libraries were constructed by adapting the described procedure based on the incorporation of deoxy-UTP during the second strand cDNA synthesis (33). Detailed protocols for short and long RNA libraries construction are presented in SI Materials and Methods. Short and long RNA libraries were sequenced by using Illumina Genome Analyzer II at the Cold Spring Harbor Laboratory facilities.
We thank Pascale Romby for providing plasmid pLUG519; Philippe Batut, Carries Davis, and Johann Schlesinger for many fruitful discussions; and Juan Valcarcel for critical reading of the manuscript. During his sabbatical stay in Cold Spring Harbor Laboratory, I.L. was supported by a "Salvador Madariaga" fellowship from the Spanish Ministry of Science and Innovation. A.T.-A. and J.V. were supported by Spanish Ministry of Science and Innovation "Ramon y Cajal" contracts. M.V. was supported by a Consejo Superior de Investigaciones Científicas JAE Predoctoral research contract. This work was supported by Spanish Ministry of Science and Innovation Grants BIO2008-05284-C02-01 and ERA-NET Pathogenomics PIM2010EPA-00606.
↵1I.L. and A.T.-A contributed equally to this work.
- ↵2To whom correspondence may be addressed. E-mail: ilasa{at}unavarra.es or gingeras{at}cshl.edu.
Author contributions: I.L., A.T.-A., and T.R.G. designed research; I.L., A.T.-A., M.V., I.R.d.l.M., M.V.-I., J.R.P., J.V., and C.S. performed research; A.D. and D.F. contributed new reagents/analytic tools; I.L., A.T.-A., A.D., V.S., and T.R.G. analyzed data; I.L., A.T.-A., and T.R.G. wrote the paper.
The authors declare no conflict of interest.
Data deposition: The sequences reported in this paper have been deposited in the Short Read Archive (accession no. SRP003288.1).
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1113521108/-/DCSupplemental.
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Made to Be Broken
From the moment we wake up in the morning there are a trillion rules — big and little — governing our lives. But sometimes, we encounter one we just can't abide by. In a pitched moment of rule-questioning, a show about rules and the people who break them.View episode details
Recently AiredView archive
Nice White Parents
Producer Chana Joffe-Walt investigates the inordinate power of white parents at one ordinary public school.
Amusement Park
We head to some of the happiest places on earth: amusement parks!
How to Be Alone
In space, in the ocean, by ourselves, or with others—we're all just figuring out how to be apart.
New to This American Life?
"When I'm trying to explain our program to someone who doesn't know it, I stammer a bunch of words like 'entertaining,' 'funny,' 'surprising plot twists,' 'true stories but not boring I swear' … and then I just give them this list." — Ira GlassView this list
Yes we have videos! Animations, music videos, speeches, our live stage shows, and more.View this list


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Make garden markers from Thrift store spoons
Old thrift store spoons, Mod collection and your favorite scrapbook papers, to garden markers, your neighbors will be envious of. These spoon plant markers are so inexpensive and look so cute!
I think, in the outdoor area an extension of the interiors, so that I can decorate, of course, like you in free as well. I remember from my childhood, used by my mother, to garden markers from old silver to try so I decided to make it myself (well, for some of my potted plants).
you used to etch the metal anyway, but my "skillz"'t that advanced, so I decided to just improvise, with Mod-smorgasbord in the open.
It's perfect for decoupage projects, the last in the sun and rain. So, what I'm saying is to keep this Marker for a long time. You can learn how below.
DIY spoon Plant markers
Collect These Supplies
Thrift store spoons
Mod hodgepodge Outdoor
Spray paint
Scrapbook paper and embellishments
This is a project that all of your neighbors is sure to love! You go outside and hammer to flatten the spoon (the round end). There was a loud Ping sound that is pleasant. Better yet, do this at 2 o'clock.
you're ready to spray paint – with a strong, free paint and for both sides.
Trace your garden markers on some scrapbook paper.
Cut out of the paper. It doesn't have to be perfect. I cut a little inside the pencil line so that some of the painted spoons could show to the edge.
Decoupage paper onto the spoon and let them dry.
Add any embellishments that you like – adhesive letters, in my case – and then paint a layer of Mod smorgasbord of Outdoor over-the-top. They give several layers, so that it's really - long-lasting! Let it dry for 24 hours before taking them outside.
you'll be surprised how this magnificent garden-is a Marker look like and how you keep. Maybe it's just me, but I think these are the best looking garden-Marker on the block. I can I say, all I paid for was the spoon. The rest of the consumables were purchased to dive from stash. Whee!!


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I'm so excited and I just can't hide it, nah, nah, nah, nah…… :)
Yes, yes, yes another expansion from Blizzard called World of Warcraft Cataclysm which brings us two new areas (kind of, Kalmidor and Eastern Kingdoms have been reformed) and two new races to play, one Horde (Goblins) and One Alliance (Worgen : werewolf like)!
See the latest World of Warcraft Cataclysm trailer video below:
Next step will be leveling from level 80 to level 85, can't wait to see the new quests and raids etc….
WoW Addict


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Science fiction and science both inspire wonder and awe, albeit in very different ways.
At its best, science fiction asks profound questions about the human condition. In contrast, science asks – and often answers – even more profound questions about the very nature of matter, space, and time.
Both science fiction and science fact explore the concept of journeying to other stars and finding life on other worlds. When it comes to interstellar travel, the truth may soon become stranger (and more amazing) than fiction.
In his February 5 public lecture webcast at Perimeter Institute, astronomer Bryan Gaensler will provide an overview of the latest thinking on interstellar travel and on the search for alien life – including why he believes the frontiers of current research may be more exciting and visionary than any fictional stories we can imagine.
Gaensler is the Director of the Dunlap Institute for Astronomy and Astrophysics at the University of Toronto, where he holds a Canada Research Chair. He has authored 400 scientific papers on cosmic magnetism, neutron stars, supernova explosions, and interstellar gas, and his popular science book, Extreme Cosmos, has been translated into six languages.
Waiting line experience
There will be a waiting line for last minute cancelled (or 'no show') seats on the night of the lecture. Doors open at 5:30pm. Come to Perimeter and pick-up a waiting line chit at the Waiting Line sign and then participate in pre-lecture activities - no need to wait in line. An announcement will be made in the Bistro at 6:45pm if theatre seats are available. Note: you must arrive in person to be part of the waiting line and be in the Bistro when the waiting line announcement is made.
No disappointments
Everyone who comes to Perimeter will be able to participate in the lecture. The public lecture will be shown simultaneously on closed circuit television in the quiet of the Time Room or the licensed comfort of the Black Hole Bistro for any members of the waiting line who are not able to get a theatre seat.
Live webcast online
Enjoy the live webcast of Perimeter Institute Public Lectures from the comfort of your own home. Join us at 7:00pm night of the lecture and be part of the ONLINE virtual audience.
For most lectures the on-demand playback will be online within 24 hours after the live event. Check our YouTube page for the playbacks.
Who can attend: Everyone
Fee: Attendance to the lecture is free, but advance tickets are required. Due to the overwhelming response to past lectures, tickets will be honoured until 6:45pm only. If you have not arrived by 6:45pm your reservation may be filled by guests in the waiting line, and you may be asked to join the end of the waiting line.
Tickets: Available online January 20 at 9:00am
Organized by: Perimeter Institute for Theoretical Physics
Location: Perimeter Institute, Mike Lazaridis Theatre of Ideas, 31 Caroline St. N., Waterloo, ON


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For Your Quarantine Sanity – May 20, 2020: Simplifying your life is so difficult.
May 20, 2020
|In Uncategorized
Slow down for twenty minutes to enjoy this beautiful film. When you come to this line … "Simplifying your life is so difficult" … I think you will feel and get something truly worthwhile. But I have to admit, I'm only now beginning to approach the maturity necessary to truly see it.


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Post Malone, G-Eazy, Kid Cudi Among 40 Hip-Hop Release Certified Gold/Platinum in One Week
Last week (March 11-19), Hip Hop's rejoiced with astounding news as 40 of the genre's releases received RIAA certifications of gold and platinum status within a single week. The list saw several a-list acts with multiple song entries including G-Eazy, Kid Cudi, Post Malone, Lil Baby among the 40 entries identified for both certified gold/platinum achievement or ascension to various levels of multi-platinum status, moving another one million equivalent album units.
Among the Hip Hop heavyweights, the list also includes well-deserved artists like Jay Rock, Rich the Kid, and Kap G with their breakout singles. The news comes on the heel of Jay Rock securing his first Grammy Award for his work with Kendrick Lamar and Future for the Black Panther soundtrack song, "King's Dead".
Post Malone's new single, "Wow" reaches Platinum status fresh off the remix release featuring Tyga and Roddy Richh.
See the complete list of the 40 certified Gold/Platinum Hip-Hop certifications below.
G-Eazy – "Been On"
G-Eazy Ft. Charlie Puth – "Sober"
Jay Rock – "Win"
Kayne West – "Violent Crimes"
Kap G Ft. Lil Uzi Vert & Gunna – "Marvelous Day"
Lil Baby – "Pure Cocaine"
Logic – "Midnight"
Shoreline Mafia – "Musty"
Shoreline Mafia – "Nun Major"
Rich the Kid – "Splashin"
G-Eazy – "Calm Down"
G-Eazy Ft. Tory Lanez & Chris Brown – "Drifting"
G-Eazy Ft. Cardi B & A$AP Rocky – "No Limit" (5x)
G-Eazy Ft. Lil Wayne & Yo Gotti – "Order More"
G-Eazy Ft. Christoph Andersson – "Tumblr Girls"
Kid Cudi Ft. MGMT & Ratatat – "Pursuit of Happiness (Nightmare)" (5x)
Kid Cudi – "Day 'N' Nite" (5x)
Kid Cudi – "Soundtrack 2 My Life" (2x)
Kid Cudi Ft. Kanye West – "Erase Me" (2x)
Lil Baby – "Freestyle" (2x)
Lil Baby Ft. Drake – "Yes Indeed" (3x)
Lil Baby & Gunna – "Close Friends"
Lil Baby & Gunna – "Drip Too Hard" (3x)
Lil Baby & Gunna Ft. Drake – "Never Recover"
Post Malone – Beerbongs & Bentleys (LP) (3x)
Post Malone – "Ball For Me"
Post Malone – "Better Now" (4x)
Post Malone – "Candy Paint" (2x)
Post Malone – "Over Now"
Post Malone – "Paranoid"
Post Malone – "Rich and Sad"
Post Malone – "Stay"
Post Malone – "Spoil My Night"
Post Malone Ft. Quavo – "Congratulations" (5x)
Post Malone – "I Fall Apart" (5x)
Post Malone Ft. Ty Dolla $ign – "Psycho" (5x)
Post Malone Ft. 21 Savage – "Rockstar" (8x)
Post Malone Ft. Swae Lee – "Sunflower" (3x)
Post Malone – "Wow"
Wiz Khalifa – Blacc Hollywood (LP)


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8 Eligibility Requirements for HARP (Home Affordable Refinance. – As such, if you have missed one or more mortgage payments, you will not be able to qualify for a HARP loan. To be eligible for a HARP loan, you must be.
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Harp 3.0 – Part of the problem, according to Donavan, is that under HARP 2.0 some government-backed loans could not be refinanced due to loan-to-value ratio (LTV) requirements. Since HUD is already "on the hook," easing LTV requirements makes sense. Part of a solution is an automated appraisal system using Fannie Mae and Freddie Mac data.
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Agency Gossip; HARP Chatter from Lenders and MI Companies; – Here are some recent underwriting happenings: Guidelines on HARP 2.0 have been released by Fannie Mae. deductibles, carrier eligibility, and allowable reimbursable expenses. Additional guidance is.
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Cannabis Companies
One of the Most Rapidly Growing Sectors in the US
Cannabis is one of the most rapidly growing sectors in the United States. Sachs Capital Group has teamed up with early pioneers creating investable solutions to seize this market. Experience is critical when investing in this competitive new horizon. Secured partnerships have mastered the complexities of cannabis, requiring thorough understanding of all aspects of the business from Federal and State regulations, to managing cultivating facilities, to product development and branding through retail dispensaries.


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The sounds of screaming riders on a roller coaster fill the air. Or maybe it was just the screeches from gulls along the beach. The smells of hot dogs cooking and peanuts roasting waft across the dunes.
On June 16,a fire destroyed the only bridge leading from the mainland to a peninsular barrier beach jutting between Long Island Sound and the salt marshes of Bridgeport and Stratford, Connecticut. Access to the peninsula became limited to either hiking from a parking lot in Stratford or by boating from Bridgeport. The line dividing the two towns runs through the peninsula.
Jump to. Sections of this page. Accessibility Help.
If so, please consider making a modest contribution that will support our efforts to bring new Connecticut stories to you! Entrance to Steeplechase Island, Bridgeport, ca. When Bridgeport annexed the borough of West Stratford inthe acquisition came with a a small acre parcel of land on a barrier island at the mouth of Bridgeport Harbor. The island, thanks to its promotion as a mysterious site tied to pirate folklore, became a popular tourist destination.
Just got an e-mail from Jackson Kuhlwith whom I've worked on a few occasions in regard to abandoned properties and decaying historical sites. In response to a recent story in the Connecticut Post about artists making over the abandoned buildings on the stretch of Connecticut barrier beach that is called Long Beach West in Stratford and Pleasure Beach in Bridgeport, he went out and investigated. From Jackson's blog :.
Father's Day of was a bad time to visit Bridgeport's Pleasure Beach. Sometime during the course of the warm summer afternoon, a motorist passing over the wooden bridge connecting the acre island to the city's east side didn't think twice before tossing a lit cigarette out his or her window. The already decaying bridge ignited and feet of its span was burnt so badly that visitors relaxing at the beach or at the Polka Dot Playhouse had to be brought back to the mainland by ferry.
A bridge connecting the peninsula to the East End burned incutting off access. And what has Mayor Joe Ganim done with that little slice of coastline since he ousted Finch and took over City Hall in ? Beginning this week, the Seaview Avenue lot, leased by the city since from the Julian family of contractors, is closed to the public.
This post was contributed by a community member. The views expressed here are the author's own. Folks of the surrounding communities sure can recall the pleasure that this beach provided. I remember so well the wooden bridge that took us across the water to the island of great entertainment.
Awesome beach for small children, just keep them off the rocks as unfortunately the fishermen bring Woke up 1 morning and asked google for nearby beaches, i live in Massachusetts, this beach was only Awesome beach for small children, just keep them off the rocks as unfortunately the fishermen bring glass bottles that often get broken so there is frequently glass on the rocks.
Hot summers, cold winters. It is one of connecticuts newer ghost towns and it is fairly sized streching about 2 miles long. It was home to a small community of beachside homes, an ammusement park, and the WICC radio tower all of which are now abandonned except for the radio tower which is accessed for maintainence by WICC via hover craft. Pleasure beach was a thriving seaside community unitll when its only connection to the mainland a wooden bridge burnt down.


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Ferguson Marine shipyard nationalised by Scottish Government amid bitter dispute with tycoon owner – and all jobs are safe
NATS ministers today nationalised a struggling shipyard amid a bitter dispute with its tycoon owner.
Finance and Economy Secretary Derek Mackay made the announcement as he visited Ferguson Marine, which is building two ferries for public-owned CalMac.
The Port Glasgow yard - which employed around 350 staff until last week - was facing administration due to costs of the new ships spiralling from the £97million agreed with CMAL.
The Scottish Government also loaned Ferguson - owned by industrialist Jim McColl - a further £45million to build ferries for the Ardrossan-Brodick and Uig-Tarbert-Lochmaddy routes.
As taxpayers are the biggest creditor, ministers are now effectively seizing control of the firm - with permanent takeover in around a month if no commercial buyer can be found.
Alex Salmond paid more than £500,000 by Scottish Government over botched sexual harassment probe
Mr Mackay said: "Public control will provide much-needed continuity of employment now and ensure the completion of the Caledonian Maritime Assets Ltd ferry contracts at the lowest possible cost to the taxpayer.
"It is absolutely essential that the outstanding contracts to build these two ferries are completed in order to sustain the Clyde and Hebrides Ferry Services network and provide vital support for the economies of our island communities.
"The alternative was for the government to stand aside while the company went into administration, resulting in the jobs being lost and the vessels not being completed. That was not an outcome I was willing to consider."
Company sources have previously said the £97million cost had doubled, mainly due to the complexity of innovative hybrid engines.
It#'s unclear how much more taxpayers will have to pay out as a result of the nationalisation, with the Scottish Government saying the firm would now undertake a "revised cost analysis to establish the actions required to complete the two CMAL vessels".
The yard was saved from collapse by Mr McColl in 2014 in a deal brokered by then First Minister Alex Salmond.
It was later handed the £97million CalMac contract, amid pressure on the ferry operator over its ageing fleet.
The Scottish Government refused to cough up a further £61 million wanted by bosses due to spiralling costs, and last week it emerged it was facing administration.
On Sunday, Mr McColl said there was "no economic sense" in the idea of nationalising the yard and claimed it would "damage the economy". He also accused SNP ministers of "abusing their power".
GMB Scotland organiser Gary Cook said: "Nationalisation secures the immediate future of the yard and that is a very welcome development, particularly after all the recent uncertainty.
"Our members were caught in the middle of a situation that had nothing to do with them and their relief will be palpable. It is five years since the yard went bust and the Scottish Government has prevented that from happening again.
"We must be clear that nationalisation will not be a quick-fix and there will be challenges. There will for example be limits to the amount of private sector work for which the yard can compete but the alternative to nationalisation was closure and that was no choice at all."
Unite union Scottish Secretary Pat Rafferty said: "Unite fully supports the actions of the Scottish Government to intervene and nationalise Ferguson Marine shipyards. It's clear at this stage that nationalisation was the only serious option on the table to save the yard and more than 350 highly skilled jobs.
"The Scottish Government have rightly acknowledged their obligations to the workforce, and the people the ferries would serve which must be acknowledged."
We pay for your stories and videos! Do you have a story or video for The Scottish Sun? Email us at firstname.lastname@example.com or call 0141 420 5200


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How to Protect Your Eyes When Using Digital Devices
Many people use digital devices like mobile phones and computers on a regular basis. For some people, their entire jobs are done on digital platforms, and this means that they are always staring at their screens. This can be very harmful to the eyes. That is why it is advisable for people who have a lot of screen time to make sure that their eyes are protected.
Tips on Protecting Eyes From Damage When Using Digital Devices
- Invest in an antiglare coating on the screen so that you avoid straining your eyes as you look at the glare that you get from the devices.
- Master the 20/20/20 rule. It means after looking at your screen for 20 minutes, you should look at something that is 20 feet from you for twenty seconds before you go back to looking at the screen again.
- When it comes to the care of eyes, it is advisable that you do not have your device so close to your face. Have it as far as possible, as long as you can read the words. Increase the fonts if necessary, to avoid straining the eye muscles as you struggle to read.
- Make sure that the brightness of your screen matches the light of your surrounding. Do not have such a sharp contrast that your eyes start feeling the pain and strain whenever you access your digital device.
- Take breaks from your device. Do not spend hours on end staring at your device that your eyes have to strain and get overwhelmed by the artificial light that is presented by the device.
- Go for regular eye check-ups to identify any eye problem before it becomes a serious issue.
- Make it deliberate to blink more often since blinking helps in producing tears for eye cleansing.
- The source of light should be behind you, and not behind the device that you are using. Placing the light source behind the device causes you to strain.


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Evoke emotions, stir the senses. Built in 1839 and then restored as a tourist destination in 1972, the Old Melbourne Gaol recently won the National Tourism Award for Best Cultural and Historical site.
Marekai Events have produced events in the Gaol, Former City Watch House and Old Magistrates for the past 10 years, and today it is one of the most sought after event spaces in Melbourne.
Events are conducted with a catalogue of tours, performers and themes. A series of cameos performers throughout events set a style and mood, and interactive and professionally produced shows - such as the 'Kelly Gang Riot' tap show- provide a level of entertainment produced specifically for events at the venue. Above and beyond the jail-house concepts, Marekai Events have consistently evolved the space to take on a myriad of backdrops transforming the spaces into an alley, devils lair, enchanted castle and hawker style market.
Marekai Events
Ph: +61 (3) 9663 4218


text: Easycare is a breed of sheep developed in the 1960s in the United Kingdom for meat. It was derived from the Nelson Welsh Mountain (a Welsh Mountain and Cheviot cross) which were crossed twice to the Wiltshire Horn, which is a wool-shedding breed. The progeny were selected for the following characteristics: wool shedding; no horns; and easy care traits. Most animals in the wild thicken up their coats in winter and then moult when spring comes, but many modern sheep breeds were selectively bred for thick coats which are retained for shearing. With the decline in the worldwide wool markets, a thick coat is now less desirable especially where the cost of shearing is higher than the value of the wool produced. In this respect, the Easycare breed is an interesting reversal of centuries of practice in sheep breed development as the sheep naturally shed their coats in spring, a process called rooing. Like lots of other sheep, easycare sheep can not live without the necessary grooming that is shearing every now and then.


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2015 A year of house sitting in review
Today, I have looked back on our year of house sitting in 2015. It has been a year of learning how house sitting works for us. We have enjoyed so many aspects, but we have missed out on some of our own activities as well. Has it all been worth it, absolutely. We have met great people, pets and housesit the most wonderful homes. We feel privilaged that people have allowed us into their homes, and all we can say is a big thank you.
House sitting statistics
14 house sits, 8 clients so some sits were repeat clients
262 days or 72% of the year house sitting
Shortest sit – 3 days
Longest sit – 58 days
Pets – dogs, chickens, snakes, fish, birds and a goose
Locations – Darwin, Palmerston, Magnetic Island
Budget 2015
Budget $200 per week between the two of us, served us well most of the time
$1,587 in accommodation costs for the year
Great things about the year
House sitting beautiful well designed properties for the tropics
Meeting great people
Living is lovely locations
Learning to love dishwashers
Developing our website
Developing our Facebook page
What we missed out on this year
Going to the gym
Cycle touring in Japan


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Also a friendly reminder, it is the season of hand watering. As you play Minor Park please be on the lookout for my staff as they fight the fires of summer. Before you strike your ball please make sure that there is some sort of contact between my staff and yourself . My guys will do there best to keep an eye out for you guys, but if need be feel free to holler or wave at them to acknowledge you are there before you hurl that torpedo rocket at them. Here is a small video that will show you why we water the way we do.
Thank you and look forward to seeing you out at Minor Park Golf Course.
Monday, June 20, 2016
Friday, June 10, 2016
The Rough.... is not rough!
Since the heat is upon us the last few days the rough has began its summer survival mode and has slowed down growing. If you have played Minor recently you may have noticed that it is becoming easier to find your ball. The course in in great shape so come out and enjoy.
Thursday, June 2, 2016
The Hazards of the Job
Last week after we were able to dry out for a day or two, Assistant Superintendent David Carlson went to mow outside the fence along Holmes road. Everything was going smoothly until his last pass.... That's when the trouble started. The Jacobsen turfcat started to slip on the hill, it was stuck. He turned off the blades, and proceeded to raise the deck, at this moment he could tell he was in serious trouble. The mower started leaning and at that moment he had to decide to bail and run for the fence. As he got to the fence he turned around in time to see the mower begin its trip down the hill. He told me that it never hit the ground when it flipped down the hill. Luckily everyone was safe and we were able to push the mower back on its side. All we had to do was get the rear tire filled with air, put some much needed oil into the motor that was lost. And then with about 5 mins of blowing out pure white smoke this piece of machinery was back to normal. Something like this doesn't happen everyday but we always have to be on our toes in this profession because you never know when something like this can cause you problems.
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Lesson Tee Protection
Due to a high volume of golfers hitting horrible errant shots off the Driving Range Tee, we decided the Lesson Tee needed more safety protec...
We were fortunate to receive an emergency grant from the city to have our sinkhole issue on #9 bridge repaired. As you saw in my previous b...
Sorry for the large gap between post, with the temps heating up and the irrigation upgrade, myself and the staff has been very busy keeping ...
As all of you know, the weather this year has been crazy! This month we broke the record in KC for the most rain ever in May. We have recei...


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Yaw Berk – Anything Else ft. Fameye. Ghanaian Prolific sensational singer Yaw Berk serves us this sweet melody captioned ANYTHING ELSE featuring the 2019 most featured Act Fameye.
Production credit goes to Paris Beatz. Download and share your thoughts with us below. DOWNLOAD MP3
DOWNLOAD MP3: Anything Else (Prod By ParisBeatz)
- Dada Hafco – Our Story ft. Fameye
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- Darkovibes X Mugeez – Y3 B3 Sei Wiase ft. Medikal (Prod By Unklebeatz)
- Mugeez (R2bees) – Ogye Me Din (Prod. By GuiltyBeatz)
- B4bonah x Mugeez (R2Bees) – Kpem


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Congratulations to Andrew Vander Muelen, the 2019 recipient of the Curtis Clark Achievement Award.
(Full Write-up) Clark Press Release 2019 Winner – Andrew Vander Meulen
Vander Meulen Wins Curtis Clark Achievement Award
Highly regarded Jersey dairy cattle breeder, Andrew Vander Meulen, 49, of Avonlea Genetics Inc., Brighton, Ontario, won this year's Curtis Clark Achievement Award at the Royal Agricultural Winter Fair in Toronto, Ont., on November 8th.
Andrew Vander Meulen and his wife Jennifer, along with their children, Taylor and Will, and Jen's parents, Jim and Liz Livock, manage Avonlea Genetics, a fourth generation family farm which has earned two Master Breeder awards. The Avonlea herd revolves around balanced, productive animals from deep-pedigreed cow families and currently consists of 20 Excellent, 33 Very Good and four Good Plus cows. The herd has earned over 300 All-Canadian nominations, resulting in 55 All-Canadian and 42 Reserve All-Canadian awards, and has exhibited grand champion cows at both the Royal Winter Fair and World Dairy Expo. The farm also boards and shows cattle for breeders and partners from five different countries. Avonlea has exported embryos to over 30 foreign countries and sells many animals privately and through consignment sales each year. Andrew and Jen also have a successful sales management business and have managed some of the highest averaging Jersey sales in North America, including their own Summer Splash series. An accomplished judge, Andrew has judged National Jersey Shows in five different countries. He has also served on numerous breed and industry committees and is a past president of Jersey Canada.
Vander Meulen is the 33rd recipient of the Clark award which recognizes a Canadian dairy cattle exhibitor who is respected by his peers for his ability, sportsmanship and dedication. The Alberta Holstein Branch established this award in 1988 to honour the late Curtis Clark of Acme Holsteins, a noted Alberta Holstein breeder, cowman and showman.
For additional information, contact Bonnie Cooper
secretary, Curtis Clark Achievement Award Committee,
904 – 12 Rockford Road, North York, ON M2R 3A2;
tel: 416-663-8515; cell: 416-579-6572; e-mail: email@example.com.
Congratulations to the breeders & exhibitors at the 2019 Westerner Dairy Showcase. 151 Holsteins were judged by Jeff Stephens, ON.
Pictures @cowsmo
HM Grand Champion
Donfield Doorman Bridget, 1st Sr 3 Yr Old, Fred Fornwald & Sons, Harold & Miriam Sweetnam, Mark Sweetnam, MB
R&W Grand Champion
Stranshome Heztry Nali-Red, 1st 5 Yr Old, Fred Fornwald & Sons, MB
Reserve R&W Grand Champion
Kozak Aussie Destiny Red, 2nd Sr 2 Yr Old, Kozak Holsteins, AB
HM R&W Grand Champion
Westcoast Action Regina, 5th 5 Yr Old, Westcoast Holsteins, BC
Intermediate Champion
Donfield Doorman Bridget, 1st Sr 3 Yr Old, Fred Fornwald & Sons, Harold & Miriam Sweetnam, Mark Sweetnam, MB
Reserve Intermediate Champion
Mosnang Solomon Liquify, 1st Jr 2 Yr Old, Mosnang Holsteins, AB & Stanhope Wedgwood, BC
HM Intermediate Champion
Benbie High Octane Ciara, 1st Jr 3 Yr Old, Benbie Holsteins, SK
Junior Champion
Trinal Love to Win, 1st Jr Yr, Westcoast Holsteins, BC
Reserve Junior Champion
Wedgwood Avalanche Rosie, 1st Summer Yr, Wedgewood Holsteins, BC
HM Junior Champion
Roselane Unstopabull Hailey, 1st Jr Calf, Wendon Holsteins, AB
R&W Junior Champion
Roselane Unstopabull Hailey, 1st Jr Calf, Wendon Holsteins, AB
R&W Reserve Junior Champion
Lavender Jordy Razzle Red, 1st Sr Calf, Lavender Farms, BC
HM R&W Junior Champion
Mosnang Ammo Whisky 7 P, 1st Int Yr, Mosnang Holsteins, Rimbey, AB
Wendon Holsteins, AB
Wendon Holsteins, AB
Premier Breeder & Exhibitor
Benbie Holsteins, SK
Herdsman Award
Mazylake Holsteins, AB
Congratulations to the exhibitors at the Youth Show!
Conformation Results
Showmanship Results
Grand Champion heifer went to Johannes Renecker (ON) with Kozak Solomon Divine (1st Junior Calf) and Reserve Champion, Hannah Vanderlinde (AB) with Lucky Meihaven Jordy Rien (1st Senior Calf). HM Champion was Jasmine Lemke (BC) with Triple S Trouble Joyride (2nd Senior Calf).
1st in Junior Showmanship was Jasmine Lemke (BC) with Triple S Trouble Joyride, 1st Intermediate and Grand Champion Showman was Ethan Nienhuis (SK) with Robella Avalanche Barbie and 1st Senior and Reserve Champion was Alice Hehli (AB) with Mosnang Undenied Access.
Thank you to Select Sires Genervations for sponsoring the youth show.,
Alberta Bred! Congrats to Skycrest Holsteins, Athabasca, AB on breeding this great cow! Skycrest Mincio Prickles EX-94 was 5th in the 5 Year Old class at World Dairy Expo 2019, she is owned by Oakfield Corners Dairy, Oakfield NY.
Photo @Ella Wright
Congrats to all of the exhibitors, the organizing committee and volunteers on another great Western Canadian Classic!
Highlights for Team Alberta were
Full results are up on the WCC website!
WCC 2020 will be held in Brandon, MB.


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Interactive VideoVideo for learning & development
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How often do you view a YouTube video to learn something that helps you do your job? Once a month? Once a week? Daily?
Research has shown that half of all employees view YouTube videos for work-related information at least once a week. Even more strikingly, nearly a quarter of them do so daily. The cognitive science behind the benefits of video learning isn't new; people tend to learn best when concepts are conveyed visually, and most of us comprehend abstract concepts better by watching demonstrations."
"Video Killed the Radio Star"
We know that video outperforms other content but doesn't replace the human element in training.
"Learning and development, human resources, corporate communications, and other leaders responsible for improving learning and communication within their organizations have been using video for years because it's a proven, powerful medium. And with video tools and technologies that now make it easier to produce, share, and search video content, a majority of companies are using video to support learning and communications more than ever before".
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Interactive Videos
VR / 360 Video
Live shoot
Property Showcase
Fashion Promo Style
Green Screen
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in Studio
3D Animation
Whiteboard Animation
2D Animation
3D Animation – Template Based


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Diesel Haze
breed by Zenseeds
Here you can find all info about Diesel Haze from Zenseeds. If you are searching for information about Diesel Haze from Zenseeds, check out our Basic Infos, Gallery or Lineage / Genealogy for this cannabis variety here at this page and follow the links to get even more information - or list all Diesel Haze Strains (12) to find a different version. If you have any personal experiences with growing or consuming this cannabis variety, please use the upload links to add them to the database!
Basic / Breeders Info
Diesel Haze is a mostly sativa variety from Zenseeds and can be cultivated indoors (where the plants will need a flowering time of ±73 days) and outdoors. Zenseeds' Diesel Haze is a THC dominant variety and is/was never available as feminized seeds.
Zenseeds' Diesel Haze Description
Limiited release in these cup days we give you last years icmag cup winner "SSSDH" crossed with our selected diesel fathers.
Oh foxy silver foxtails, that will cut through even the most hazed mind like butter.
Super value for your money. Don't miss this, you will keep it.
10-11 weeks flowering, sativa dominant hybrid.
Recommended indoors or way south of 55°N.
Genetics: ojd's SSSDH x Dansk Diesel
Diesel Haze Gallery
Here you see the latest Diesel Haze photos, uploaded from our users! Altogether we've collected 8 pictures from Zenseeds' Diesel Haze, check out our Diesel Haze gallery to view them all.
Diesel Haze Lineage / Genealogy
- Diesel Haze »»» SSSDH OJD Cut x Dansk Diesel
- SSSDH OJD Cut
- »»» Super Silver Haze x Sour Diesel
- Super Silver Haze
- »»» {(Haze x Haze) x Skunk #1} x {(Haze x Haze) x NL #5}
- (Haze x Haze) x Skunk #1
- Haze x Haze
- Haze
- Haze (specified above)
- Haze x Haze
- (Haze x Haze) x NL #5
- Sour Diesel
- Sour Diesel
- »»» Original Diesel x DNL
- Original Diesel
- »»» Chemdawg x {MassSuperSkunk x SensiNL}
- MassSuperSkunk x SensiNL
- MassSuperSkunk
- Super Skunk Probably
- »»» Skunk #1 x Afghanistan
- Skunk #1
- Skunk #1 (specified above)
- Afghanistan »»» Indica
- Super Skunk Probably
- SensiNL
- »»» NL #1 x NL #2 x NL #5
- Afghanistan »»» Indica
- Northern Lights
- Afghanistan Indica Probably »»» Indica
- Northern Lights
- NL #5 (specified above)
- MassSuperSkunk
- Chemdawg
- Unknown Indica »»» Indica
- »»» {RFK Skunk x Hawaiian} x Northern Lights
- RFK Skunk x Hawaiian
- RFK Skunk
- Unknown Skunk
- Skunk #1 (specified above)
- Unknown Skunk
- RFK Skunk
- Northern Lights (specified above)
- Sour Diesel
- Dansk Diesel
- Sour Diesel IBL (specified above)
Map of the Diesel Haze Family Tree
Click to show all parents of Diesel Haze in our dynamic family tree map.
Upload your info about this strain here:
Do you know something more about Zenseeds' Diesel Haze? Please help to make this database better and upload/connect your information here!
Pictures speak louder than words! Upload your "Diesel Haze" Photos here and help other growers to get a better impression of this variety.
You have grown Diesel Haze together with another variety? Please fill out our Strain VS. Strain direct comparisation form!
User Reviews
Our strain reviews are multilingual, searchable and can be very detailed - including data about the grow, aroma, effects and taste! Please upload your Diesel Haze Review here to help the other seedfinder users!
Medical Values
You have experience with the medical qualities of Diesel Haze? Sharing your information here maybe can help other people!
You've stumbled upon a Diesel Haze related thread into a forum or growers community? Connect it here and enable othe users to find this information fast and easy!
You found a related video with additional information or grow-infos about Diesel Haze on YouTube? Please connect it here to the strain info page!


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By LARRY VAUGHT
Just remember that even though I won't be on Sunday Morning Sports Talk after Tuesday's decision by iHeart Media to discontinue the show I did with Mark Buerger and Anthony White, I will still be on radio.
I am on WPBK (102.9 FM, wpbkfm.com) every other Thursday from 7:15-8 a.m. with Tim Estes talking UK sports. This week's guest is Sporting News columnist Mike DeCourcy. And I am not going away on WKYB (107.5 FM, wkybfm.com) with Jayme Phillips on Thursdays from 9-10 a.m. This week's guest include Lee Cruse and Hayley Harmon talking about their new show starting next month on WTVQ-TV (Channel 36).
I also will continue to be a guest on the Leach Report with Tom Leach each Tuesday between 9-10 a.m. and hopefully will get to guest host occasionally like I have for the last few years. Who knows, Michael Bennett might even have me on some and now I can do guest spots with Larry Glover on WVLK (590 AM) also.
But not being on Sunday morning on WLAP is going to be different. I have been doing the show 17 or 18 years I think, including the last 11 with Buerger. Before the hosts I worked with were Leland Conway and Jim Tirey (ironically iHeart Media also cut Conway loose Tuesday). Remember at one time All-American Kyle Macy was part of the show and then White, a former all-SEC player at UK and NFL player, joined the crew.
I went years without missing a show, including calling in from vacations. Because of a terrific deal with Verizon, I even called the show from Switzerland and Italy to do shows. Why do that? Because I liked what I was doing and did not consider it work.
Fortunately, WLAP execs always let me get off air about 10:45 a.m. so I could head to church and not worry about what my late father would be thinking as he looked down if I skipped church to do a radio show.
I cannot tell you how much I enjoyed the calls or meeting folks who listened when I was at games or anywhere else. And did the folks love the Buerger rants — and so did Anthony and I.
My biggest regret is that we didn't know last week was our last show and didn't get a chance to say thanks on air to all who listened even though I understand now it is customary in radio not to let those being relieved of their jobs to have a final show.
What helped a lot Tuesday was hearing from so many listeners and friends. I lost count of the text messages and calls and there was no way to keep track of the mentions on Twitter. Here are a few I pulled off and really liked I wanted to share just to give you an idea.
Sarah White: "Listened to you every Sunday on the way to church. They must have blindsided everyone. I am sorry for each of you, and I am sorry for me."
Patrick McWhorter: "A regular listener in south Alabama. Sure am going to miss y'all on the way to church."
Chris Adams: "Never called, but enjoyed listening and communicating through here with all of you. Sundays will be quite a bit slower around here now."
Robin Huffman: "I hate that the show is ending. Always looked forward to the witty banter every Sunday morning"
Dave Hopewell: "I do hate to hear this Larry. I couldn't listen in all the time but when I could I always enjoyed it. Wonder what the next chapter will bring?"
Susan Tyler Witten: "So bummed. I streamed the show from Louisville every Sunday while getting ready for church (& I occasionally was late due to an interesting segment!). Lots of my friends (& husband!) were affected by the changes. Wanted you to know I will really miss the show."
Robert Hill: "Loved listening to you guys, every Sunday morning in the fall after a big football game while setting around the family breakfast table before Church. We are sad to hear of this news."
Randy Webb: "I have listened and read your stuff for years. Growing up in Winchester, going to miss the whole gang."
And perhaps my favorite social media message of them all was from Jon Kelly Johnson.
"Will miss getting ready for church, drinking coffee and listening to you guys on Sunday morning. It something I really enjoyed. My wife would listen too in our bathroom … and make comments. What a shame. I'll miss hearing you Larry."
And I will miss talking to all of you on Sundays … and wondering just what Johnson's wife and others might have been saying and thinking.


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Thursday October 10, 2013 - 7:00 pm
Center for Urban Horticulture
UW Northwest Horticultural Society Hall
3501 NE 41st St, Seattle WA
6:30 pm Refreshments
7:00 pm Plant Sale
7:30 pm Program and Meeting
Daniel Winkler "Plants of the Tibetan Plateau (and a Few Mushrooms)"
Author, geographer and ethno-mycologist Daniel Winkler has spent 15 years working and traveling in Tibet and the Himalayas.
He is the author of field guides to Edible Mushrooms of the Pacific Northwest and of California. He grew up collecting and eating wild mushrooms in the Alps and has been foraging 15 years in the PNW and working as mushroom educator and guide. Daniel trained as a geographer and ecologist and works as researcher and NGO consultant on environmental issues of the Tibetan Plateau and Himalayas, including forestry, conservation and revegetation.


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Each home, loft, apartment suite, level and so on needs little kitchen apparatuses to meet the day by day needs of singles, couples and families over the world. Where might we be without little kitchen machines we depend on regular? How great is the inclination to go to your kitchen in the first part of the day, evening or night to make breakfast, lunch, supper or simply making little tidbits or light dinners realizing that you have the apparatuses and machines to make readiness simple?
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Air Fryer for nearly oil free cooking
Blenders - variable speed for beverages and smoothies and inundation style for cooking and preparing
Espresso creators - multi-cup and single cup assortments
Stewing pot/Slow Cookers - incredible for occupied families w/restricted time to make dinners!!
Profound Fryer - wings, seared finger nourishments, and so forth.
Electric blade for simple cutting
Coffee Machine in the event that you like that additional shock toward the beginning of the day!
Level top Grill - incredible for steaks, chicken, cleaves and so forth when it's too cold to even think about grilling outside
Nourishment Processor - observe any cooking show and they generally have a processor for sauces, dressings, salsa, and so forth. Extraordinary item to consolidate fixings in next to no time.
Juicers for solid beverages
Microwave - that's all there is to it?
Blenders - stand up assortments for hands free blending on your heating/baked good ventures and hand-held styles for littler blending needs
Weight Cookers - extraordinary for across the board dinners with next to zero oil/oil.
Rice Cooker - no compelling reason to continue checking the rice for dishes and natively constructed sushi. Set it and overlook it!
Toasters and Toaster Ovens - magnificent machine for warming remaining nourishments
Waffle Irons - who doesn't care for waffles, extraordinary for the children's as well!
The little kitchen apparatuses recorded above just as a lot more can be purchased online at any number of destinations @ various costs. Ensure you do a little research on your specific apparatuses.
Purchasing little kitchen machines online can be simple and fun. It will spare you the issue of heading to the store, checking every specific apparatus against the contender, sitting around and gas, and so on. Most stores online will offer free transporting and comes back to make your buy streamlined and bother free! Search for authentic destinations (your antivirus program on your PC ought to have the option to check locales as you click on them), do a few correlations on what sort of little kitchen machine you need and away you go!!


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