text:
How long does it take to fill a 6ft pool?
The time to fill with a standard hose was about 3 hours and need to lift it to smooth out the bottom as it fills.
What is a good size for a swimming pool?
If you're not sure how big of a pool you need, looking at what's popular is a good place to start. Most rectangular pools are about twice as long on one side as they are on the other, with an average depth of around 5.5 feet. Typical swimming pool dimensions are 10 x 20, 15 x 30, and 20 x 40.22 мая 2019 г.
How many feet does a pool need to be away from a house?
A swimming pool in residential zones or any zone used for residential purposes may occupy a portion of the required rear yard, but in no case shall the outer walls of the pool be less than five (5) feet from an interior side property line or rear property line or building or be less than ten (10) feet from any side …
Is it OK to fill a pool overnight?
Hi, with 12″ of water in the pool you should be fine to let it fill all night. The stretching should be done and the liner tight against the wall. If so, let it go. There is some air gap between the liner and the wall in a few areas.
How long does it take to fill up a swimming pool?
12-24 hours
Is 20×40 a big pool?
This is by no means the largest swimming pool size you can build or install, but it is among the most popular of the larger pool sizes. … A 20×40 concrete pool can cost you over $100,000, but this cost varies by project.
Is 18 ft pool big enough?
The 18′ was plenty enough room for 2-3 people floating around and good bit of people standing, but that's it, no diving or swimming laps.
How wide is a 25m swimming pool?
Main Pool Length – 25m plus 0.03m minus 0.00m. Width – 6 by 2m lanes with a preferred space of 0.5m (minimum 0.2m) outside the first and last lanes. Depth – 1.0m to 2.0m. A moveable floor is an option which may be provided to give a deep water pool of 1.8m to 2m all over depth.
How do I clean my pool naturally?
Baking soda, also known as sodium bicarbonate is naturally alkaline, with a pH of 8. When you add baking soda to your pool water, you will raise both the pH and the alkalinity, improving stability and clarity.
How long can water sit in pool without chlorine?
24-48 hours
How do I keep my 8ft pool clean?
Here, then, are the best ways to keep the kiddie pool water clean throughout the summer.
- Drain and refill. …
- Purchase a kiddie pool cover that fits. …
- Try the Aqua Broom. …
- Get a Scumbug. …
- Use the pool skimmer every day. …
- Use chemicals formulated for small pools.
9 мая 2018 г.
Which direction should a swimming pool face?
But where should I point them?
- Most people like to point the jets in a unified direction, clockwise or counterclockwise. …
- Conventional wisdom dictates that most pool owners should point the jets up to create vigorous ripples in the water. …
- Your skimmer(s) are designed to skim surface area.
18 мая 2016 г.
How do you know if you can put a pool in your backyard?
Your Ultimate Pool Planning Checklist
- Go outside and check for wires running over your backyard. …
- Check for utility lines running under your property. …
- Choose a spot that's at least 100 feet from any body of water. …
- Assess the terrain of your yard. …
- Check for any easements on your property.


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The Bachelorette viewers were so disheartened by the actions of contestant Karl Smith during the second episode of The Bachelorette that they took to Twitter to call him out for starting unnecessary drama between the men and series lead Katie Thurston.
Throughout the episode, fans felt that Karl had something up his sleeve. This became apparent when he was able to secure some one-on-one time with Katie and let her know some unfounded information about her potential suitors.
Karl's motivations for joining the show have come into question after he was seen in a trailer for this season where he claimed in a sit down with producers, in the quest for Katie's heart, "I didn't come her for the bromance I came here for the followers."
His intentions to get Katie's attention came early on in the episode when he was upset he didn't take home the trophy after a contest where the men proved to Katie why they were the best lover. Mike (the virgin) won that. Karl later vowed to win a group date rose but was edged out by Thomas.
At a cocktail party later in the episode, Katie asked the men to let her know if there was something going on in the house she should know about.
This gave Karl the opportunity to speculate to Katie that there were men on the series that did not have the best intentions for being there, stirring up the pot for drama.
Katie flipped out
During his time with Katie, Karl told her that there were men there who were not on the show for the right reasons.
When pressed by the brunette beauty for more information, Karl could not present any.
After their talk, Katie approached her suitors and asked them if they were there for her, as she had time and again made her intentions from what she wanted from The Bachelorette process crystal clear, using several expletives before leaving the room upset.
Karl later admitted to his castmates that he told Katie to stay vigilant, but still declined to name anyone he had warned her about because he wanted to give the people a chance to come forward.
The men were angered by Karl's deceptive move and told him so, believing he was not on the show with the best intentions either. The episode ended without a rose ceremony.
Fans reacted to the drama
Viewers reacted to The Bachelorette drama Karl incited on Twitter.
One fan used a photo depicting the cartoon cat Tom from Tom and Jerry to illustrate their statement which quoted Karl in a moment from the show where he spoke to Katie about the other contestants as seen below.
"Karl is the Victoria of this season," wrote a second viewer, who likened Karl to the actions of Bachelor "mean girl" Victoria from Matt James season.
A third Twitter user a meme featuring Jane Lynch from Glee which alluded to his intentions to create a toxic environment during the rose ceremony.
The Bachelorette airs Mondays at 8/7c on ABC.More: Bachelor, Bachelorette, The Bachelor, The Bachelorette

subset: commoncrawl


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Gene therapy trials would benefit greatly from the use of noninvasive imaging to determine the location, magnitude, and time course of gene transfer. Somatostatin receptor subtype 2 (SSTR2) has been used as a reporter probe for γ-camera imaging of gene transfer in animal models. PET has greater sensitivity than γ-camera imaging and therefore would have an advantage for the imaging of SSTR2 gene transfer. Methods: An adenovirus (AdHASSTR2) carrying sstr2, which encodes an N-terminal hemagglutinin epitope, was used for evaluating SSTR2 gene transfer. The somatostatin analog Demotate 1 (Tyr3-octreotate conjugated to the 1,4,8,11-tetraazaundecane chelator) was used for chelation of the positron emitter 94mTc (half-life, 52 min) and targeting to SSTR2. Gene transfer was evaluated in vitro with A-427 non–small cell lung cancer cells after infection with AdHASSTR2 by 94mTc-Demotate 1 binding and internalization assays. In vivo biodistribution and microPET studies were conducted with mice bearing A-427 tumor xenografts directly injected with AdHASSTR2 to determine the tumor localization of 94mTc-Demotate 1. Results: 94mTc-Demotate 1 bound with high affinity and was internalized rapidly into AdHASSTR2-infected A-427 cells. Biodistribution studies showed uptake of 94mTc-Demotate 1 in tumors infected with AdHASSTR2 (4.0 percentage injected dose per gram [%ID/g] at 2 h) and background uptake in tumors infected with a control adenovirus (0.8 %ID/g at 2 h). The uptake of 94mTc-Demotate 1 in AdHASSTR2-infected tumors was greater than the uptake in all other tissues, except for the kidneys and the SSTR2-positive pancreas. MicroPET imaging showed similar results, with clear uptake of 94mTc-Demotate 1 in AdHASSTR2-infected tumors, background uptake in control tumors, and clearance through the kidneys. Conclusion: These studies show that the positron-emitting somatostatin analog 94mTc-Demotate 1 could be used to determine SSTR2 gene transfer by microPET imaging, a result that will improve the sensitivity of the SSTR2 reporter gene system.
In more than 600 clinical gene therapy trials worldwide in the past 15 y, approximately 3,500 patients have been treated (according to the Journal of Gene Medicine Web site, at www.wiley.co.uk/genetherapy/clinical). More than 500 of the trials have been conducted in the United States, and 400 have been carried out in the context of the treatment of cancer. Retroviral vectors were used as gene delivery vehicles in 34% of the protocols, whereas adenoviral vectors were used in 27%. It is clear that gene therapy will be evaluated clinically as an approach for the treatment of numerous human disorders despite obstacles that must be overcome before gene therapy can live up to its potential (1). One obstacle that must be overcome is the ability to determine whether gene transfer has occurred in an individual patient (2). Biopsies are taken to determine whether gene transfer has occurred, but this process is invasive and does not yield spatial or temporal patterns of gene expression. Therefore, noninvasive imaging of gene transfer would determine whether gene transfer has occurred in a patient, what tissues have been transduced, and the magnitude and time course of gene expression (2).
Several groups are developing methods for noninvasively imaging gene transfer. In general, these methods have focused on nuclear imaging, MRI, and optical imaging (3). Each imaging technology has various advantages and limitations. We have focused on nuclear imaging with the human somatostatin receptor subtype 2 (SSTR2) gene as a reporter gene, although many nuclear imaging studies of gene transfer have been carried out with the herpes simplex virus type 1 thymidine kinase (4,5), the dopamine 2 receptor (6,7), and the sodium iodide symporter (8,9) genes as reporter genes. Five somatostatin receptor subtypes (SSTR1–SSTR5) have been cloned, with alternate splicing of SSTR2 to yield SSTR2A and the C-terminally truncated form, SSTR2B (10). All of the receptor subtypes have high binding affinity for the natural somatostatin 14 or somatostatin 28 peptides. Somatostatin receptors are members of the G protein–coupled receptor family and have 7 transmembrane-spanning domains consisting of 3 extracellular loops, termed E1, E2, and E3 (11). The 5 receptor subtypes are highly homologous between mice and humans and are expressed in the brain, gastrointestinal tract, pancreas, kidneys, and spleen (12,13). Human SSTR2A (hereafter referred to as SSTR2) has been used for the imaging of gene transfer by use of γ-camera imaging and SPECT (14–17). These previous studies used the radiolabeled somatostatin analogs 111In-diethylenetriaminepentaacetic acid-octreotide (Octreoscan; Mallinckrodt), 99mTc-P829 (NeoTect; Diatide, Inc.), and 99mTc-P2045 (Diatide, Inc.) to demonstrate localization in a tumor after intratumoral injection with an adenovirus or intraperitoneal injection with a vaccinia virus carrying the sstr2 gene.
Because PET is at least 1 order of magnitude more sensitive than SPECT, in part because of the compromise between spatial resolution and sensitivity attributable to the required collimator for SPECT (18,19), it would be advantageous to image SSTR2 gene transfer by PET. In recent years, there has been interest in somatostatin analogs radiolabeled with PET radionuclides, such as 18F, 86Y, 66,68Ga, and 64Cu (20–24). All of these radionuclides have advantages and disadvantages for use in the labeling of somatostatin analogs. In this study, we used the positron emitter 94mTc (half-life, 52 min; β+, 72%; Eβ+max, 2.5 MeV) produced with a high specific activity on a biomedical cyclotron (25). The somatostatin analog Demotate 1 (Tyr3-octreotate conjugated to the 1,4,8,11-tetraazaundecane chelator) (Fig. 1) was used for chelation of 94mTc and targeting to SSTR2. This analog was previously radiolabeled with 99mTc for γ-camera imaging of SSTR2-positive tumors (26). The binding and internalization of 94mTc-Demotate 1 were evaluated in vitro after infection of cells with an adenovirus (AdHASSTR2) encoding SSTR2 that contains the influenza virus hemagglutinin (HA) sequence at the N terminus of the receptor. The expression of SSTR2 then was evaluated in vivo with tumor-bearing mice in biodistribution and microPET imaging studies. To our knowledge, this is the first study to use PET to evaluate SSTR2 gene transfer.
Cell Line and Adenovirus
The A-427 non–small cell lung cancer cell line was purchased from the American Type Culture Collection and maintained in Eagle minimum essential medium supplemented with 1% nonessential amino acids, 1% sodium pyruvate, and 10% heat-inactivated fetal bovine serum. All cell culture reagents were obtained from Invitrogen, except for fetal bovine serum, which was purchased from Sigma Chemical Co. The cells were maintained in a humidified atmosphere with 5% CO2 at 37°C. AdHASSTR2 was constructed and propagated and virus titers were determined as previously described (27), and the virus was stored at −80°C until use in cell culture and animal studies.
Production of 94mTc
94mTc was produced by a method similar to that previously described (25,28,29). Briefly, MoO3 (Isoflex USA) (∼60 mg), enriched with 91.0% 94Mo, was pressed into a platinum disk and covered with aluminum foil. The disk was irradiated (14.7 MeV, 4 μA, 45 min) on our biomedical cyclotron, and 94mTc was produced by the 94Mo(p,n)94mTc nuclear reaction. After irradiation, the 94mTc was separated by heating of the target in a quartz apparatus at 1,000°C and allowing the 94mTc to condense in the temperature region of 300°C–400°C. The 94mTc was removed in NaOH (0.1 mmol/L) and purified over an Alumina N Sep-Pak Light cartridge (Millipore) to yield the desired 94mTcO4− in saline. The product was >95% 94mTcO4−, as demonstrated by use of instant thin-layer chromatography (ITLC) silica gel plates (Pall Corp.) with acetone as the solvent. The yield of 94mTc was 1,295 MBq (35 mCi)/μA·h at the end of bombardment (EOB), and isotopic impurities were measured on a multichannel analyzing Ge γ-spectrometer (Canberra). The saline containing 94mTcO4− was used immediately after isolation for the radiolabeling of Demotate 1.
Synthesis and Radiolabeling of Demotate 1
Demotate 1 was synthesized as previously described (26). For radiolabeling, Demotate 1 was dissolved in acetic acid (10 mmol/L) at 2 mg/mL, and 15 μg were added to a vial containing 25 μL of phosphate buffer (0.5 mol/L), 3 μL of trisodium citrate buffer (0.1 mol/L), and 196 MBq (5.3 mCi) of 94mTcO4− (200 μL). To this solution, 20 μg of SnCl2 (dissolved in ethanol at 5 mg/mL) were added, and the reaction mixture was incubated for 15 min at room temperature. The reaction was stopped by the addition of 4 μL of HCl (1 mol/L) and 25 μL of ethanol. Radiochemical purity was determined by use of ITLC with acetone and 1:1 methanol:ammonium acetate (1 mol/L) as the solvents. The plates were analyzed by use of an imaging scanner (Bioscan), with 94mTc-Demotate 1 remaining at the origin in the acetone system and moving with the solvent front in the ammonium acetate system.
Saturation Binding Assays
A-427 cells were infected with AdHASSTR2 at 10 plaque-forming units (pfu) per cell and harvested 2 d later to prepare membrane homogenates as previously described (30). Twenty-five micrograms of membrane homogenate were added to each well of a 96-well Multiscreen Durapore filtration plate (Millipore) and rinsed twice with cold buffer [N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES) at 10 mmol/L, MgCl2 at 5 mmol/L, ethylenediaminetetraacetic acid at 1 mmol/L, and 0.1% bovine serum albumin (pH 7.4)]. Various concentrations (0.5−100 nmol/L) of 94mTc-Demotate 1 were added to the wells in triplicate in the presence or absence of Tyr1-somatostatin (Sigma Chemical Co.) as an inhibitor such that the final volume in each well was 110 μL. The plate was incubated at room temperature for 30 min with shaking, the wells were rinsed twice with buffer, and the filters were dried and removed. Counts in samples were determined by use of a model 8000 automated well-type γ-counter (Beckman). Data were analyzed by use of the 1-site binding equation in Prism software (GraphPad) to generate equilibrium dissociation constants (Kd) and maximum binding capacities.
Internalization Assays
A-427 cells were infected with AdHASSTR2 at 10 pfu per cell and then used to seed 6-well plates 24 h later. One day after seeding (2 d after adenovirus infection), 94mTc-Demotate 1 (1 nmol/L) in 1 mL of internalization medium (Dulbecco minimum essential medium, HEPES at 30 mmol/L, l-glutamine at 2 mmol/L, sodium pyruvate at 1 mmol/L, and 1% bovine serum albumin) was added to each well and incubated at 37°C for 5, 15, 30, 60, and 120 min. An excess (>1,000 nmol/L) of Tyr1-somatostatin was added to half of the wells to demonstrate specific binding and internalization. The cells were rinsed twice with phosphate-buffered saline and then with Hanks balanced salt solution containing sodium acetate at 20 mmol/L (pH 4.0) to remove surface-bound radioactivity. The cells then were harvested with 1 mL of sodium borate (10 mmol/L) containing 10% sodium dodecyl sulfate and counted in the γ-counter along with the acid washes to determine the internalized radioactivity and the cell surface radioactivity. After 24 h, the amount of protein in each well was determined by use of a bicinchoninic acid (BCA) protein assay kit (Pierce). The data are presented as the specific amount of peptide (Tyr1-somatostatin–blocked wells subtracted from nonblocked wells) bound to the cell surface or internalized, normalized to the amount of protein per well.
Animal Biodistribution Studies
All animal studies were performed in compliance with guidelines for the care and use of research animals established by the Washington University Animal Studies Committee. Homozygous 6- to 8-wk-old female nude (nu/nu) mice (Charles River Laboratories, Inc.) were implanted subcutaneously in each rear flank with 107 A-427 cells mixed 1:1 with Matrigel (Becton Dickinson) such that each mouse had 2 tumors. Three weeks after tumor cell inoculation, tumors on 1 side of the mice were injected directly with 109 pfu (30 μL) of AdHASSTR2, and the other tumors were injected with 109 pfu (30 μL) of a control adenovirus (AdGRPR, encoding the gastrin-releasing peptide receptor), as previously described (31). Two days after the adenovirus injections, 0.6 MBq (17 μCi; 100 ng) of 94mTc-Demotate 1 was injected via the tail vein. The mice (n = 6) were sacrificed by cervical dislocation 1 and 2 h later. In another experiment, the same procedures were followed, except that 1 group of mice was injected in the tail vein with 94mTc-Demotate 1 and another group was coinjected with 50 μg of unlabeled Demotate 1 as an inhibitor. Both groups (n = 5) were sacrificed 1 h after injection. The blood, lungs, liver, spleen, kidneys, muscle, bone, pancreas, and tumors were harvested, weighed, and counted in the γ-counter. Samples were corrected for radioactive decay to calculate the percentage injected dose per gram (% ID/g) of tissue by comparison with a standard representing the injected dose.
MicroPET Imaging
MicroPET imaging was performed with an R4 microPET scanner (Concorde Microsystems Inc.). The R4 microPET scanner has a field of view of 8 cm axially by 11 cm transaxially and is capable of a spatial resolution of 2.3 mm and an absolute sensitivity of 1,020 counts per second per 0.037 MBq in the middle of the field of view. The same animal model as that described above was used for microPET imaging, except that the tumors were implanted in the axillary thorax. In these studies, the mice (n = 3) were injected via the tail vein with 18.5 MBq (500 μCi; 3.4 μg) of 94mTc-Demotate 1, anesthetized 1 and 2 h later with 1%–2% isoflurane, positioned supine, immobilized, and imaged. The tumor and kidney standard uptake values (SUVs) were generated as previously described (32). Briefly, the regions of interest encompassing the entire tumor or tissue on the microPET images were measured to generate the radioactivity concentration, which was decay corrected to the time of injection. This value then was multiplied by the mouse body weight (grams) and divided by the injected dose (Bq) to yield the tissue uptake normalized to injected dose per gram of body weight: (Bq/mL) × (weight [g]/injected dose [Bq]).
Statistical Analysis
All of the data are presented as the mean ± SEM. The Student t test (2 tailed) was performed with Prism software to determine statistical significance at the 95% confidence level, with P < 0.05 being considered significantly different.
Production of 94mTc and Radiolabeling of Demotate 1
The major radionuclide impurity of <7% was from 94gTc (half-life, 293 min; β+, 10.5%; Eβ+max, 811 keV), with all other radionuclide impurities constituting <0.4%. The positron contribution of 94gTc was not greater than 10% up to 4 h after EOB. The processing time from EOB until 94mTcO4− was ready for radiolabeling of Demotate 1 was typically 45 min. This process resulted in a recovery of radioactivity that ranged from 81 to 274 MBq (2.2–7.4 mCi) and an effective specific activity for 94mTc-Demotate 1 of 14,874 ± 4,884 MBq/μmol (402 ± 132 mCi/μmol; n = 8). The radiochemical purity of 94mTc-Demotate 1, as determined with the ITLC system described above, was >95.5%. The total time from EOB until 94mTc-Demotate 1 was ready for in vitro or in vivo studies was 75 min, and it should be noted that unlabeled Demotate 1 was not separated from 94mTc-Demotate 1 before use.
Saturation Binding Assays
A representative saturation binding curve and Scatchard transformation are shown in Figures 2A and 2B, respectively. The data show that 94mTc-Demotate 1 bound to a single class of binding sites with a Kd of 9.6 ± 2.8 nmol/L and a maximum binding capacity of 9,200 ± 2,700 fmol/mg of protein, as determined from 3 independent experiments. Because 94mTc-Demotate 1 consists of a mixture of radiolabeled Demotate 1 and unlabeled Demotate 1, the Kd represents a composite of these 2 species.
Internalization Assays
A-427 cells infected with AdHASSTR2 at 10 pfu per cell demonstrated time-dependent internalization of 94mTc-Demotate 1 (Fig. 3). The amount of specifically internalized 94mTc-Demotate 1 was 285 fmol/mg of protein at 5 min and increased to 1,022, 1,558, 1,842, and 2,407 fmol/mg at 15, 30, 60, and 120 min, respectively. The amount of internalized 94mTc-Demotate 1 was significantly greater (P < 0.0001) at all time points than the amount of surface-bound 94mTc-Demotate 1 (<182 fmol/mg at all time points), except for the 5-min time point.
Animal Biodistribution Studies
The tissue uptake of 94mTc-Demotate 1 in tumor-bearing mice receiving intratumoral injections of AdHASSTR2 is shown in Figure 4A. The uptake of 94mTc-Demotate 1 in tumors injected with AdHASSTR2 was 3.5 ± 0.8 %ID/g and 4.0 ± 0.6 %ID/g at 1 and 2 h after the administration of 94mTc-Demotate 1, respectively. This uptake was significantly higher (P < 0.03) than the uptake at both time points in all other tissues, except for the kidneys, the SSTR2-positive pancreas, and the liver at 1 h. There was rapid blood clearance of 94mTc-Demotate 1, with 1.2 ± 0.1 %ID/g remaining at 1 h and 0.8 ± 0.1 %ID/g remaining at 2 h. The uptake of 94mTc-Demotate 1 was <1.8 %ID/g in all other tissues, except for the pancreas, kidneys, and SSTR2-positive tumors at both time points. The ratios for the AdHASSTR2-injected tumors and other tissues at 1 and 2 h are shown in Figure 4B. The data show that all of the ratios were >2 at both time points, except for the kidney and pancreas ratios, which were both <1. The ratios also all increased from 1 h to 2 h in all tissues.
SSTR2-mediated uptake of 94mTc-Demotate 1 in tumors injected with AdHASSTR2 was demonstrated by coinjection of an excess of unlabeled Demotate 1 and by comparison with a tumor injected with AdGRPR as a control adenovirus (Table 1). The blocking study showed a significant decrease (P < 0.003) in the SSTR2-positive tumor uptake of 94mTc-Demotate 1 at 1 h (5.4 ± 0.8 %ID/g) with coinjection of the blocking agent (1.6 ± 0.3 %ID/g). The tumor injected with AdGRPR showed 94mTc-Demotate 1 uptake that ranged from 0.8 ± 0.1 %ID/g (Table 1) to 1.5 ± 0.3 %ID/g (Fig. 4A) at 1 h and was 0.8 ± 0.1 %ID/g at 2 h; these uptake values were significantly lower (P < 0.03) than the uptake values at those time points for the AdHASSTR2-injected tumors. The uptake of 94mTc-Demotate 1 also significantly decreased (P < 0.0001) from 21.3 ± 1.5 %ID/g without the blocking agent to 0.2 ± 0.03 %ID/g with the blocking agent in the pancreas. The only other tissues to show a significant decrease after administration of the blocking agent were the liver (P < 0.02) and the kidneys (P = 0.045). In addition, the radioactivity in the pancreas decreased 37% from 1 h to 2 h compared with no decrease in SSTR2-positive tumors. This finding likely was attributable to the high enzymatic activity in the pancreas, which led to higher tumor-to-pancreas uptake ratios at later time points. The only significant differences in the uptake of 94mTc-Demotate 1 between the 2 experiments conducted at 1 h (Fig. 4A; Table 1) were found in the blood, pancreas, and AdGRPR-injected tumor.
MicroPET Imaging
Tumor-bearing mice were imaged 1 and 2 h after the injection of 94mTc-Demotate 1, and 1-h coronal and transaxial microPET images are shown in Figures 5A and 5B, respectively. The data show clear uptake of 94mTc-Demotate 1 in the axillary tumor injected with AdHASSTR2 and background uptake in the tumor injected with AdGRPR. Also, as in the biodistribution studies, the clearance of radioactivity through the kidneys and excretion through the bladder were clearly visualized. The pancreas was not observed in Figure 5 because of the slice of the microPET image shown. SUVs also were determined for both tumors and the kidneys (Fig. 5C). The data show that there was significantly greater (P < 0.007) uptake of 94mTc-Demotate 1 in the AdHASSTR2-injected tumor than in the AdGRPR-injected tumor at both time points and that the uptake in the kidneys was significantly greater (P < 0.02) than the uptake in the AdHASSTR2-injected tumor at both time points. Comparison of the uptake in the AdHASSTR2-injected tumor with that in the AdGRPR-injected tumor and with that in the kidneys (by SUV and biodistribution analyses) is shown in Figure 5D. There was good agreement between the SUV analysis and the biodistribution analysis at 1 and 2 h for the ratio of the AdHASSTR2-injected tumor uptake to the AdGRPR-injected tumor uptake. These values differed by <12% at each time point. The differences between the SUV and biodistribution analyses were greater when the AdHASSTR2-injected tumor uptake was compared with the kidney uptake. A 35% difference between the values was observed at 1 h, whereas a 65% difference was observed at 2 h.
Initial studies of imaging of gene transfer with the SSTR2 gene as a reporter gene were carried out with probes radiolabeled with γ-emitters for γ-camera or SPECT analysis (14–17). These studies were carried out with 99mTc- or 111In-labeled probes for evaluating the tumor expression of SSTR2 after animals were administered a gene therapy vector carrying the sstr2 gene. SSTR2-specific uptake of these probes was observed in the tumors after direct injection of adenovirus vectors into the tumors or intraperitoneal injection of a vaccinia virus. In addition, Rogers et al. evaluated an adenovirus that contains SSTR2 with the 9-amino-acid HA sequence inserted in the N terminus of the receptor (27). That study demonstrated that insertion of the HA sequence into the N terminus of SSTR2 did not have an adverse effect on ligand binding to SSTR2 and that the HA sequence could serve as an alternative epitope for the binding of anti-HA antibodies. However, because PET has better sensitivity than SPECT at a given resolution, this system could be improved through the use of probes radiolabeled with positron emitters.
94mTc has the same chemistry as the γ-emitter 99mTc (the most important γ-emitting radionuclide) but is a positron emitter that can be used for PET studies. It is readily produced on a biomedical cyclotron at high specific activities for the radiolabeling of various biomolecules. Nickles et al. showed that 94mTc could be used in myocardial perfusion studies to obtain results that correlated well with those of standard PET perfusion studies (33). Griffiths et al. showed that an Fab′ fragment of an anti–carcinoembryonic antigen monoclonal antibody could be readily radiolabeled with 94mTc for potential PET of carcinoembryonic antigen–positive tumors (34). Luyt et al. used 94mTc to radiolabel an estrogen derivative to evaluate estrogen receptor expression in rats by microPET imaging (25). The advantages of using 94mTc over other positron emitters are that it has a relatively simple chemistry for the labeling of peptides and antibodies (compared with 18F, 76Br, or 124I), imaging studies must be conducted on the same day as radiopharmaceutical administration because of the short half-life (this is not necessarily true for longer-lived isotopes), and the short half-life should lead to radiation doses lower than those observed with longer-lived isotopes. However, 94mTc requires a biomedical cyclotron for production and has less-than-ideal physical properties for PET because of the high positron energy. This high positron energy leads to an intrinsic spatial resolution loss of 3.3 mm (35) but is still expected to provide resolution superior to that of corresponding studies with 99mTc and SPECT (34). Thus, 94mTc-labeled biomolecules can be used to model tracer kinetics, measure dosimetry, and determine structure–activity relationships by PET before their wider application with 99mTc and SPECT.
In this study, 94mTc was used to radiolabel the somatostatin analog Demotate 1. Demotate 1 was previously radiolabeled with 99mTc and has demonstrated good SSTR2-mediated tumor uptake in preclinical and clinical studies (26,36). These studies showed that tumor uptake could be observed within 15 min of 99mTc-Demotate 1 injection and that maximum tumor uptake and tumor/organ uptake ratios could be observed by 1 h after injection. These data are important for the radiolabeling of Demotate 1 with 94mTc because its 52-min half-life requires rapid target tissue uptake and clearance from nontarget organs. Radiolabeling of Demotate 1 with 94mTc was accomplished with high radiochemical purity in 15 min with an effective specific activity of 14,800 MBq/μmol (400 mCi/μmol). The effective specific activity was not optimized in our studies because we used 15 μg of Demotate 1 for all of the radiolabelings and never more than 273.8 MBq (7.4 mCi) of 94mTc. Therefore, it is expected that higher effective specific activities can be achieved by optimizing these parameters.
The saturation studies (Fig. 2) showed that 94mTc-Demotate 1 binds with a high affinity (9.6 nmol/L) to A-427 cells infected with AdHASSTR2 at 10 pfu per cell. Maina et al. showed that 99mTc-Demotate bound to SSTR2-expressing AR42J rat pancreatic carcinoma cells with a Kd that was approximately 100 times higher (70 pmol/L) (26). This result likely was attributable to 99mTc-Demotate having a higher affinity for SSTR2 than unlabeled Demotate. This finding is similar to the findings of studies showing that somatostatin analogs complexed with Tc/Re have a 20- to 30-fold higher affinity than noncomplexed analogs (37). In these saturation studies, Maina et al. ensured that all of the Demotate contained a Tc atom by using 99gTc as a carrier so that the final product was 99m/99gTc-Demotate. Because of the short half-life of 94mTc, we chose not to add 99gTc as a carrier and performed the studies with a mixture of 94mTc-Demotate 1 and unlabeled Demotate 1. This mixture is similar to many 99mTc "kit" formulations of radiopharmaceutical agents that contain an excess of the biomolecule. The expression of SSTR2 in A-427 cells infected with AdHASSTR2 at 10 pfu per cell in this study (∼9,200 fmol/mg) was similar to that previously described (∼33,000 fmol/mg) (27). These values for SSTR2 expression are 50–100 times higher than those reported with the commonly used AR42J cell line.
Rapid internalization of 94mTc-Demotate 1 was observed (Fig. 3), with 285 fmol/mg being internalized by 5 min. This internalization increased over the time course of the experiment, with 2,407 fmol/mg being internalized by 2 h. Nonlinear curve fitting showed that the curve reached a maximum at ∼3,300 fmol/mg. The initial velocity of internalization at this concentration of 94mTc-Demotate 1 was ∼49.7 fmol/mg/min, as determined by linear regression (r2 = 0.96) over the first 30 min. The amount internalized was greater than the amount surface bound, which remained constant over the course of the experiment. These results are similar to those reported previously for the internalization of 99mTc-Demotate 1 into AR42J cells, which showed rapid internalization that started to plateau by 15 min and reached its maximum by 30 min. Rapid internalization of radiolabeled peptides has been shown to be important for them to be used as diagnostic or therapeutic agents.
Biodistribution studies showed that there was good uptake of 94mTc-Demotate 1 in tumors injected with AdHASSTR2, with values of 3.5–5.4 %ID/g at 1 h after injection and 4.0 %ID/g at 2 h. This tumor uptake was comparable to that in other studies with the same A-427 tumor model and an intratumoral injection of adenovirus. Zinn et al. showed that at 48 h after the intratumoral injection of an adenovirus expressing SSTR2, tumor uptake at 3 h after the injection of 99mTc-P829 was 2.5 %ID/g (14). In another study by Zinn et al., 10.2 %ID/g was observed in tumors at 26 h after the injection of 99mTc-P2045 (15). Maina et al. (26) showed greater uptake of 99mTc-Demotate in AR42J cells at 1 h after injection (∼25 %ID/g) than was found in AdHASSTR2-infected A-427 cells in this study. This difference in uptake may be explained by the fact that only ∼10% of A-427 cells express SSTR2 after adenovirus infection compared with all natively expressing AR42J cells (14). Also, as discussed earlier, the specific activity of 94mTc-Demotate 1 was not optimized in our studies, a factor that may have led to lower tumor uptake, and was ∼2.5-fold lower than the specific activity described for 99mTc-Demotate 1. It is also clear from these studies that tumor uptake was SSTR2 mediated. The reduction in the liver and kidneys after coinjection of the blocking agent may have been attributable to low levels of expression of SSTR2 in these tissues. SSTR2 messenger RNA has been detected in mouse kidneys and liver, with SSTR2 protein being detected in the glomeruli of the kidneys (38,39).
As discussed above, several somatostatin analogs have been evaluated for γ-imaging of SSTR2-mediated gene transfer. This is the first study to use a somatostatin analog radiolabeled with a positron emitter to monitor SSTR2-mediated gene transfer by PET. Figures 5A and 5B clearly show the accumulation of 94mTc-Demotate 1 in tumors infected with AdHASSTR2 and background radioactivity in tumors infected with the control, AdGRPR. As in the biodistribution studies, the kidneys also were observed, with subsequent clearance through the bladder. The pancreas was not observed in these particular slices of the microPET images, but a strong pancreatic signal was observed in others (data not shown), a finding also consistent with the findings of the biodistribution studies. Analysis of the microPET images showed that there was good agreement between the SUVs obtained from microPET studies and the % ID/g values obtained from biodistribution studies. The SUV relationship between AdHASSTR2-injected tumor and the kidney is not as consistent with the biodistribution as the relationship with the control tumor, possibly because of the high levels of radioactivity in the kidneys and the high positron energy of 94mTc making the SUV measurements in the kidneys less accurate.
It is anticipated that these studies will be expanded to use somatostatin analogs radiolabeled with other positron emitters. Because 94mTc has a short half-life and is not commonly produced on most cyclotrons, other positron emitters will have more widespread applicability. The 18F-labeled somatostatin analogs could be used at most institutions with cyclotrons but still suffer from a relatively complex synthesis strategy (23,40). Somatostatin analogs radiolabeled with 86Y or 64Cu have the advantage of the long half-life of these isotopes for regional shipping and imaging at later time points, whereas 68Ga has the advantage of being generator produced for use at many institutions. Nevertheless, these studies demonstrate that 94mTc-Demotate 1 can be used for PET of SSTR2-mediated gene transfer and that 94mTc-Demotate 1 could be used to model gene transfer characteristics with PET before radiolabeling with 99mTc for additional SPECT studies.
This study shows that SSTR2-mediated gene transfer can be monitored by PET. Specific tumor localization of 94mTc-Demotate 1 in mice was visualized by microPET imaging after intratumoral injection of AdHASSTR2 but not after intratumoral injection of a control adenovirus. This investigation represents a first step in demonstrating that SSTR2 can serve as a reporter probe for PET gene transfer. The use of somatostatin analogs radiolabeled with other positron emitters and the development of better ligands that target the HA epitope may improve this system further.
This work was supported by Department of Energy grant DE-FG02-02ER63476 and the Department of Radiation Oncology, Washington University School of Medicine. The production of 94mTc at Washington University School of Medicine was supported by NCI grant R24 CA86307. Small-animal imaging at Washington University School of Medicine was supported by NIH grant 5 R24 CA83060. The authors gratefully acknowledge Dr. Petros Sotiriou for his contribution to the synthesis of Demotate 1, Deborah Sultan for the production of 94mTc, Nicole Fettig for the biodistribution studies, Ron Chen for tumor cell implantation, and Jerrel Rutlin and Lori Strong for the microPET imaging and data analysis. Heather Bigott is also thanked for helpful discussions on the production of 94mTc, and Carolyn Anderson is thanked for critical reading of the manuscript.
Received Jun. 8, 2005; revision accepted Aug. 11, 2005.
For correspondence or reprints contact: Buck E. Rogers, PhD, Department of Radiation Oncology, Washington University School of Medicine, 4511 Forest Park Blvd., Suite 411, St. Louis, MO 63108.
E-mail: rogers{at}radonc.wustl.edu


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There are allegations that Prince Harry and Meghan Markle using the Queen for their own benefits. They attempted to abuse their link with Queen Elizabeth for the purpose of personal advantage. These allegations have drawn criticism.
According to reports, Angela Levin writes about the royal family and allegedly disclosed this information. She started by bringing up the Prince Harry and Meghan Markle's alleged efforts of using their link with the Queen in the beginning of her speech.
She went on to add, "You see, the title pretty much says it all. But Meghan didn't want the title. However, she and Harry don't want to be royals, they didn't want to do anything royal. The only thing they have interested in was exploiting the title so that they could earn a lot of money."
"And I believe that it's extremely interesting actually because you can't simply utilise people for your own goals. You actually have to prove things. I think that it's quite intriguing actually because of the fact that."
Before drawing to a close, the expert praised Netflix for being patient with Meghan Markle and Prince Harry. She also noted that when a lot of money has concerned with a huge organisation, they're not foolish. They're only going to wait so long. I believe that they have shown an extraordinary amount of patience.
Read Also: More than 170 obsessive stalkers become threat for Royal Family


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You Put Our Town On The Map!
you put our town on the map!
There seems to be an unspoken agreement between the freeks and the pigs in the community, In which we leave them alone and they leave us alone. Of course, there re occaisonal busts (see this issue), but on the whole, the repression scene is very light – at least for white people. lts a totally different scene in the Black community. The incedent a couple of weeks ago in West Park where the pigs vamped on some thirteen year old Black kid was no isolated case. The Black community has presented fourteen demands to Ann Arbor Shitty Council. none of which have been responded to. Among the demands are the dismissal of the officers involved in the West Park incident, dismissal of all charges against those arrested there, and turning West Park into a Black cultural center. This last was already been happening and is the reason for continuous pig harassment at the park – somehow the men can't relate to a lot of Black people getting together...
Anyway, the whole thing has recieved virtually no support from the freek community, despite publicity in the Argus. Why, we say as we take another toke off our joint, should we worry about it? After all, the pigs don't bother US that much. . . A couple of things . One, is that our lives and the lives of Black people in this country are totally tied together. The Black liberation struggle has always inspired and given leadership to white youth. The pigs respond to Black energy with brutal racist repression, and, a couple of weeks ago, the people responded with a righteous ripoff/kidnapping of a pig judge and jurors in Marin, California. Bringing it all back home to Ann Arbor, the people of the Black community are really getting together and moving on the honks that run this town. They deserve our total support in this. The reason the freek community hasn't felt some of the same repression is because we've sat by and let the pigs shit all over black people, we've kept in or place and not really made any trouble for them. If we keep on in this manner, we shouldn't be surprised if the righteuos wrath of Black people starts coming down on us too.
"The best way to support a revolution is to make your own" – Abbie Hoffman. We have to create a new society, a real community in this town, defining our own lives and terms of struggle instead of just existing in the space set aside for us by the pigs. We must create a new society out of the ruins of the old – the dialectic between creation and destruction of the pigsytem must be constant. We have to remember that the pigs are our enemies, are the enemies of ALL the people, however nice they may come on. In fact, the nicest ones are the worst, people like Staudemeir who get next to you, smiling all the time, telling you how much they love you, while all the time gathering information for files and future busts. The University of Michigan sits intact in the middle of our territory, a center of imperialism where they make neat little things like the infra red tracing device that killed Che Geuvera. Our brothers and sisters are turning to smack instead of revolution, maybe because the concept of revolution is still abstract to them. What we have to do is make it a reality on all levels, starting with things like the tribal councils and making them really a basis for the community, and going on from there. We should learn from our sisters and brothers in places like Berkley, Isla Vista, Boston, who have followed the example of the Blacks and the Vietcong and are taking the struggle to a higher level- moving into the streets and beyond into firebombing pig and pig institutions and businesses, all the time building togetherness and tightness with each other as the fight grows, and the need for that love of each other grows. It's time we injected some of that same love and energy into our own lives, its time we started figuring out where we should move – its time to put our town in the map! !
Freeing John Sinclair
Old News
Ann Arbor Sun


text:
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text:
A person was hit by passenger train heading from Nyíregyháza to Cegléd. The accident happened between Újfehértó and Hajdúhadház on Tuesday morning, Mávinform announced.
Máv wrote on their website: during the site investigation, trains can only run on one of the tracks, so delays of 10-15 minutes are to be expected.
According to the information of the disaster management: a person was hit by a train in Téglás, near Kurucz street. The professional firefighters from Hajdúböszörmény and the municipal firefighters from Újfehértó were alerted to the accident. The units, under the direction of the Disaster Response Operations Service, helped about fifty passengers on the train to transfer to the relief flight.


text:
Nature and Empire in Ottoman Egypt
An Environmental History
Part of Studies in Environment and History
- Author: Alan Mikhail, Yale University, Connecticut
- Date Published: January 2013
- availability: Available
- format: Paperback
- isbn: 9781107640184
Other available formats:
Hardback, eBook
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In one of the first ever environmental histories of the Ottoman Empire, Alan Mikhail examines relations between the empire and its most lucrative province of Egypt. Based on both the local records of various towns and villages in rural Egypt and the imperial orders of the Ottoman state, this book charts how changes in the control of natural resources fundamentally altered the nature of Ottoman imperial sovereignty in Egypt and throughout the empire. In revealing how Egyptian peasants were able to use their knowledge and experience of local environments to force the hand of the imperial state, Nature and Empire in Ottoman Egypt tells a story of the connections of empire stretching from canals in the Egyptian countryside to the palace in Istanbul, from the forests of Anatolia to the shores of the Red Sea, and from a plague flea's bite to the fortunes of one of the most powerful states of the early modern world.Read more
- First ever environmental history of the Middle East
- Uses both Ottoman Turkish and Arabic archival materials from over a dozen collections in Turkey and Egypt
- Elucidates the importance of Egypt in the Ottoman Empire
- Winner, 2011 Samuel and Ronnie Heyman Prize for Outstanding Scholarly Publication, Yale University
Reviews & endorsements
Review of the hardback: 'This book adds an important new dimension to the historiography of Ottoman Egypt. The author makes very intelligent use of Ottoman administrative documents and Muslim court records from a variety of Egyptian locales in order to situate this critical region within the new cutting-edge scholarship on the role of the environment and natural resource management in history.' Jane Hathaway, Ohio State University and author of The Arab Lands under Ottoman Rule, 1516–1800See more reviews
Review of the hardback: 'Alan Mikhail deploys an impressive array of environmental history insights. He asks new questions and comes up with startling answers.' Richard W. Bulliet, Columbia University
Review of the hardback: 'Nature and Empire in Ottoman Egypt offers a history of the Ottoman world like no other. The force of environmental processes, the lived detail of peasant life, and the emergent forms of modern governmental power interact in this highly original account of early modern Egypt.' Timothy Mitchell, Columbia University
Review of the hardback: 'Through admirable and painstaking research, Mikhail has explored a new and fascinating aspect of Ottoman Egypt, using a timeframe that spans a transitional period, which allowed him to draw comparisons and provide original comments and provocative opinions that will stimulate future debate. This book is highly recommended.' Doris Behrens-Abouseif, Journal of the Economic and Social History of the Orient
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Product details
- Date Published: January 2013
- format: Paperback
- isbn: 9781107640184
- length: 382 pages
- dimensions: 229 x 152 x 22 mm
- weight: 0.56kg
- contains: 7 b/w illus. 9 maps 7 tables
- availability: Available
Table of Contents
Introduction: an empire by nature
1. Watering the Earth
2. The food chain
3. The framework of empire
4. In working order
5. From nature to disease
6. Another Nile
Conclusion: the imagination and reality of public works
Appendix: citations for cases enumerated in Tables 1 through 4.
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text:
Paige Spiranac reduced to tears after 'golf Karen' criticized her 'highly inappropriate outfit' on course
PAIGE SPIRANAC was reduced to tears after a 'golf Karen' ripped into her "highly inappropriate outfit".
The ex-golf pro, 29, told followers of her Playing A Round podcast she was left shaken by the dressing down from a moody player.
Paige was visiting a new course and had read up on their dress code, only for the woman to confront her about her threads on the range.
The influencer has 3.6m Instagram followers and recently told fans she dresses a certain way because she has 'an image to uphold'.
Spiranac maintains she is always respectful to the wishes of whichever course she might be playing on.
But that didn't seem a good enough excuse for the complaining player, who reduced a "shaking" Paige to tears.
Spiranac revealed: "I wore something that I knew I wouldn't get in trouble with.
"I had a collar, I zipped the zipper up so there was no cleavage showing, it came about mid-thigh, and I felt like it was a very appropriate outfit.
"This lady goes up to one of the workers and I hear her talking to this guy, and I can't quite make it out. Then all of a sudden I hear, 'what she's wearing is inappropriate, we have rules, I'm going to talk to her'.
"I tee off and I'm shaking because I never want to be in a position where I'm making a bad first impression.
"I [like to] look inappropriate because I know the misconceptions on me to begin with so I really try to dress as appropriately as possible."
Paige revealed that she made it successfully through the round after a shaky start - but was then ambushed in the car park.
She continued: "She comes straight up to me, stops me in the parking lot, and she goes, 'woman to woman, you need to hear this.
"You look cute but that is highly inappropriate, we have rules here and you cannot wear that outfit," she remembered.
"She was so condescending and so rude to me.
"She was talking to me like I'd never been on a golf course before and like I wasn't rich enough to know these rules, and I'm just a stupid young girl that looks s****y on the golf course.
"I was just out there minding my own business and she makes a point to make me feel so small.
Most read in Golf
"It was one of the most traumatizing experiences that's happened to me on a golf course in a long time.
"I literally cried all the way home."
Paige blasted Phil Mickelson and Dustin Johnson this summer over their decision to join the Saudi-backed LIV Series.
And she whipped fans into a frenzy by showing them how to make a Memorial Tournament milkshake.
Spiranac has a huge global following and regularly does Q&A's, revealing her most personal secrets.


text:
I can rap Pap.
Wear a backward cap.
Make you clap.
Even make you toe-tap.
Act like a stone-cold sap.
My woman she was a mantrap.
She was full of crap a hell brat.
And made me lose my map.
To the gap.
I was not a bit hap.
Then she sat on Fat's lap.
Soon she take a perm nap.
You doubt that then stand pat.
You'll see you snitch rat.
Shut up, you another mother or you be next at bat.
By the way, your ass be fat.
You Hillbilly cat
Get the fuck out or get got gnat.
Can you dig that maggot.
I'm just like 2pac an uncapped cat.


text:
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text:
Patil and Medvedev watched the 90 minute classical and folk arts performance held at the world famous Bolshoi Theatre last night organised as part of the ongoing "Year of India in Russia" festival.
The concert titled 'Sahasra Patra', which means a lotus of thousand petals, was performed in a kalideoscopic backdrop and kept the audience spell-bound receiving spontaneous response.
"It was one and a half hour complete immersion in the Indian culture," Medvedev told Patil at the end of the performance.
He said the friendly ties with New Delhi had stood the test of time and would never be overshadowed by differences and conflicts, and the relationship with India was always one the most important foreign policy priorities of Moscow.
Patil said: "we are assured that India lives in the hearts of every Russian. Similarly, I can assure you that Russia lives in our hearts as a land and a people who share our feelings of mutual respect and abiding friendship".
The event started with noted Hindustani classical musician Shruti Sadolikar Katkar rendering a composition in praise of God.
This was followed by Nadeswaram from Tamil Nadu, Talavadhyam and Kathakali from Kerala, Shanka Vadyam from Orissa, Flower and Thunchem from Jammu and Kashmir, Bisukamsale from Karnataka and Kinnauri Nati from Himachal Pradesh among others.
"Very innovative and a sheer magnificient show. All kudos go to Bansi Kaul, renowned director and choreographer, who helped put together the show," well known lyricist Gulzar said.
The festival was organised by the Indian Council of Cultural Relations (ICCR) and came as part of the year-long celebration.
The performance was telecast live by Russia's federal television channel Kultura (culture). The Year of Russia festival was held in India last year.
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text:
HTML5 Live New York City, NY November 1, 2011
Jonas Jacobi, TweetCEO and Co-Founder, Kaazing
Biography: Jonas Jacobi
As co-founder and chief executive officer of Kaazing Corporation, Jonas Jacobi sets the company's business strategy and oversees all aspects of Kaazing's operations and mission to become the world-wide leader in real-time software.
Before co-founding Kaazing Jonas Jacobi worked as VP of Product Management responsible for the product management and marketing strategy for Brane Corporation, a startup company in Silicon Valley. A native of Sweden, Mr. Jacobi has worked in the software industry for more than sixteen years. Prior to his appointment as vice president for Brane, he worked 8 years for Oracle as a Java EE and open source Evangelist, and product manager responsible for the product management of JavaServer Faces, Oracle ADF Faces, and Oracle ADF Faces Rich Client in the Oracle JDeveloper team.
Mr. Jacobi is a frequent speaker at international conferences and has written numerous articles for leading IT magazines such as Java Developer's Journal, JavaPro, AjaxWorld, and Oracle Magazine. Jonas is co-author of the recently published book Pro JSF and Ajax: Building Rich Internet Components, (Apress).
Blog: http://www.jroller.com/jonasjacobi/
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Book: Pro HTML5 Programming: Powerful APIs for Richer Internet Application Development
Twitter: http://twitter.com/jonasjacobi
Presentation: TweetOpening Keynote - HTML5 Is Here. Are You?
Abstract will be announced soon


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The fact that it's next week! Ooooh I cannot wait!
im excited to see the Heroes of the Storm finals and the wowhead party again this year!
I'm most excited for the lovely ladies hosting it. :)Also the prizes.
Winning a frostwolf cub! One Christmas gift out of the way.
If I was going, I would be most excited about getting a blanket! Those look sooo comfy, and perfect for snoogling up with while I play!
This all sounds exciting! Its nice that you guys do these things for us. The claw machine is my favourite, even though I have no talent with the thing hahah. The plushies are absolutely adorable too. And those funko pop figures look awesome (illidan, I am definitely prepared)! The hype is real (*^ω^*) !
the claw machine
I'm excited for all the stuff animals! Got to love the plush!
Giant plushies!
The claw machine of course!
I wish I could attend to stuff like that, everything sounds fun. I want to claw that pepe up!
really wanna be there but too far away, u guys have fun for me too ^_^excited to see a great community... imma be on Twitch with foodies and drinks!
Definitely the giant plushes. I have a small version of that gryphon, time to meet the big guy.
Claw machines sounds great, also I'm happy to check the stream, since I live too far. :)
Well, not gonna be there so... I'm excited for you guys telling us about it... I guess.
I'm most exited about the stream, that way I can at least catch some of the party :D
Im excited for the livestream! (Since I sadly wont be able to make it to the party :/ )
What am I looking forward to? THE CAKE!Question: are all the pins prize wheel only? Hoping to collect them all >.>
The giant plushies, claw machine and pins sound awesome, wish I could go!
I'm most excited to see which beverage names made the cut. That was a pretty fun challenge to tackle.


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Headstart activities
Discover Pinterest's 10 best ideas and inspiration for Headstart activities. Get inspired and try out new things.
Want to learn how to homeschool preschool? This post will help you get started! Sharing tips, ideas, and favorite reources to help you out!
These are great portable sensory activities for autism! They are small enough to take in your purse, and provide great sensory input...
dorothy jones saved to Headboard
Christian craft projects for kids: ideas for Sunday school, vacation Bible school, CCD classes and home school.
Anita Kralj saved to Sunday school crafts
Back to School Activity Binder (Preschool)- fun busy bag ideas for toddlers and preschoolers! Download the PDFs to make a interactive binder. Great for classrooms or at home! Shapes, colors, tall to short, abc, alphabet learning activities. #backtoschool #classroomideas #activitybinder #binder #preschooleducation #preschoolfun #craftymorning
A great way to slowly teach your kid(s) how to spell. Start with small work and slowly go for bigger words.
It's Always Autumn saved to Everything for kids
Who are your helpers? It's easy to see with this cheerful show of hands. Encourages teamwork and self-esteem as students take responsibility for their classroom Features 10 class job cards (pledge leader, postmaster general, tech spec
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Mar 16, 2020 - ❤ 11 + Simple Preschool Activities for 3 Year Olds Lesson Plans Ideas ☞ What are your favorite activ
simple crafts A simple season trees q-tip painting activity for preschoolers simple crafts
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Shower Mist from Plant Oil
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Refresh your shower experience with an Aromatic In-Shower Spa Experience. Start your day or night feeling centered and relaxed.
If you are looking for the aroma in your shower to be a bit stronger than the shower steamer squares, the Shower Mist Oil is the perfect option for you. The aroma will linger well after you've finished your shower.
Hand-blended in house to ensure its quality and performance.
Soon to be a daily ritual in every shower, this mist is the perfect way to provoke an uplifted mood, all you have to do is breathe.
Lasts for 200+ pumps.
Therapeutic Essential Oil
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The Foundations of New India
Foundations of New India, cultivating her own ethical, spiritual values are the ideals India is working today. It is a revolution undertaken through democratic processes based upon her own historical foundations.
The author of this book on the foundations of new India was widely traveled, well read and eminent historian.
The subject has been examined in proper historical perspective. It is an essential reading for historians, political scientists and policy makers.
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The Evolution of British Policy Towards Indian States 1774-18588.00 $ 8.89 $Add to cart
Asia And Western Dominance16.20 $ 18.00 $Add to cart
India and China: A Study of Cultural Relations35.10 $ 39.00 $Add to cart
A Survey of Indian History42.30 $ 47.00 $Add to cart
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The Cambridge Economic History of India: C. 1200-C.1750 (Volume 1)14.40 $ 16.00 $Add to cart
Syamukapu: The Lhasa Newars of Kalipong and Kathmandu45.00 $ 50.00 $Add to cart
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Bibliographic information
Title The Foundations of New India
Author K.M. Panikkar
Format Hardcover
Date published: 02.05.2019
Edition 1st ed.
Publisher Life Span Publishers and Distributors
Language: English
isbn 9788193909447
length viii+259p.
Subjects History


text: The Texas Governor's Committee on People with Disabilities congratulates the Texas Special Olympics athletes for their outstanding performance at this year's Games. The 2018 Special Olympic USA Games were held in Seattle July 1-6. A complete list of the 2018 Texas Team can be found at Special Olympics Texas. Continue reading "Special Olympics Celebrates 50 Years"


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Seven Oaks Kennel
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Canvas On Demand transforms your pictures, digital photo enlargements, traditional photo enlargements, family portraits, senior portraits and pet portraits into beautiful giclee style canvas prints. Choose from our Photorealistic or Light Brush Strokes techniques as well as various levels of retouching. With regular and express shipping, a photo on canvas makes for a unique birthday gift, anniversary gift, Christmas gift, Valentines Day gift, Mothers Day gift or Fathers Day gift.
Canvas on Demand LLC


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An archaic bronze style squat cloisonné vase decorated to the central section with stylised entwined guei dragons, beneath a band of open lappets to the neck, the ground a mottled hue of various shades of green and brown (simulating a buried Han dynasty bronze vase) The silver rims with impressed silver mark, and signed Ando Jubei.
Taisho period.
12 x 10in (30 x 26cm)
« Return to Winter Collection 2020


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Today, I have gathered together ten books which I read quite some time ago, but which I rarely see written or spoken about. The books here are a mixture of fiction and non-fiction from different periods. I wanted to bring a little more attention to these quite excellent tomes, and really hope that you find something which takes your fancy.
1. Basil and Josephine by F. Scott Fitzgerald
'Basil and Josephine charts the coming of age of two privileged youths from quiet Midwestern towns, Basil Duke Lee and Josephine Perry – based on Fitzgerald himself and a combination of his first love Ginevra King and his wife Zelda. As one struggles to gain the acceptance of his peers and becomes consumed by ambition, the other finds herself obsessed by teenage crushes and has to confront the pitfalls of popularity.
Written for the Saturday Evening Post while the author was working on Tender Is the Night, these stories form a realistic and entertaining portrait of two young adults in the 1910s, fascinating both for the autobiographical insights they provide and the timeless satire that Fitzgerald's fiction has become synonymous with.'
2. Mad, Bad and Sad: A History of Women and the Mind Doctors from 1800 by Lisa Appignanesi
'Mad, bad and sad. From the depression suffered by Virginia Woolf and Sylvia Plath to the mental anguish and addictions of iconic beauties Zelda Fitzgerald and Marilyn Monroe. From Freud and Jung and the radical breakthroughs of psychoanalysis to Lacan's construction of a modern movement and the new women-centred therapies. This is the story of how we have understood mental disorders and extreme states of mind in women over the last two hundred years and how we conceive of them today, when more and more of our inner life and emotions have become a matter for medics and therapists.'
3. Dinosaurs on Other Planets by Danielle McLaughlin
'A woman battles bluebottles as she plots an ill-judged encounter with a stranger; a young husband commutes a treacherous route to his job in the city, fearful for the wife and small daughter he has left behind; a mother struggles to understand her nine-year-old son's obsession with dead birds and the apocalypse. In Danielle McLaughlin's stories, the world is both beautiful and alien. Men and women negotiate their surroundings as a tourist might navigate a distant country: watchfully, with a mixture of wonder and apprehension. Here are characters living lives in translation, ever at the mercy of distortions and misunderstandings, striving to make sense both of the spaces they inhabit and of the people they share them with.'
4. Rain: Four Walks in English Weather by Melissa Harrison
I chose Melissa Harrison's Rain: Four Walks in English Weather to read during my final Dewey's 24-Hour Readathon. It is truly lovely; within its pages, Harrison takes four countryside walks around various parts of England, and in different seasons. Her writing is lovely, and she makes the most of discussing the ways in which rain affects particular landscapes, and how the animals which live within them have adapted – or not, as the case may be. Rain is geographically, geologically, historically, and biologically interesting, and provides several nods to works of literature throughout. Charming, thought-provoking, and lovely, particularly when one considers it in tandem with its glossary, which provides one hundred words for different kinds of rain around the United Kingdom.
5. The High Places: Stories by Fiona McFarlane
'What a terrible thing at a time like this: to own a house, and the trees around it. Janet sat rigid in her seat. The plane lifted from the city and her house fell away, consumed by the other houses. Janet worried about her own particular garden and her emptied refrigerator and her lamps that had been timed to come on at six.
So begins "Mycenae," a story in The High Places, Fiona McFarlane's first story collection. Her stories skip across continents, eras, and genres to chart the borderlands of emotional life. In "Mycenae," she describes a middle-aged couple's disastrous vacation with old friends. In "Good News for Modern Man," a scientist lives on a small island with only a colossal squid and the ghost of Charles Darwin for company. And in the title story, an Australian farmer turns to Old Testament methods to relieve a fatal drought. Each story explores what Flannery O'Connor called "mystery and manners." The collection dissects the feelings–longing, contempt, love, fear–that animate our existence and hints at a reality beyond the smallness of our lives.
Salon's Laura Miller called McFarlane's The Night Guest "a novel of uncanny emotional penetration . . . How could anyone so young portray so persuasively what it feels like to look back on a lot more life than you can see in front of you?" The High Places is further evidence of McFarlane's preternatural talent, a debut collection that reads like the selected works of a literary great.'
6. A Little Love, A Little Learning by Nina Bawden
'It is 1953 and Joanna, Kate and Poll, who are eighteen, twelve and six, are living in a riverside suburb of London with their mother Ellen and their stepfather Boyd, the local doctor. Then the past arrives to upset the present in the person of Aunt Hat, a gossipy old friend whose husband has been imprisoned for assulting her, and who seems to bring news from a different world of chaos and drama. The real danger, however, comes not from Aunt Hat's indiscretions but from the girls themselves.'
7. Portrait of a Family by Richmal Crompton
'Happily married for thirty years with three children that have long since grown up, Christopher Mainwaring finds himself at a total loss following the death of his beloved wife, Susan. Yet the joyful marriage he remembers may not have been all it seemed, for no one in the family knows of the troubling words his wife uttered to him from her death bed . . .
Alluding to a possible affair that took place many years ago with a close family friend, the grieving widower is haunted by visions of Susan's infidelity and seeks to find out the truth. In his quest to unearth his wife's potential duplicity, Christopher finds himself looking to his children's complex lives for answers: Joy who is now married with children and concerns of her own, the professionally inept but kind-hearted Frank and his neurotic wife Rachel, and Derek, whose delusions of grandeur with his struggling business causes much distress for his long-suffering wife, Olivia.
Portrait of a Family by Richmal Crompton provides universal reflections and intimate insights into the dynamics of family life with a startling clarity that will stay with the reader long after the final page has been turned.'
8. Heating and Cooling: 52 Micro-Memoirs by Beth Ann Fennelly
'The 52 micro-memoirs in the genre-defying Heating & Cooling offer bright glimpses into a richly lived life. They build on one another to arrive at a portrait of Beth Ann Fennelly as a wife, mother, writer, and deeply original observer of life's challenges and joys. Some pieces are wistful, some poignant, and many of them reveal the humor buried below the surface of everyday interactions. Heating & Cooling shapes a life from unexpectedly illuminating moments, and awakens us to these moments as they appear in the margins of our lives.'
I had not heard of Beth Ann Fennelly's Heating and Cooling before, but stumbled across it on my online library catalogue and borrowed it immediately. I love fragmented memoirs, and this is a particularly interesting one. Through each of these 'micro-memoirs', Fennelly reveals herself little by little. The entries are amusing, and sometimes quite touching; Fennelly's approach is fresh and enjoyable. There is such depth and consideration to the writing, and I will definitely be looking out for Fennelly's books in future.
9. Undying: A Love Story by Michel Faber
'In Undying Michel Faber honours the memory of his wife, who died after a six-year battle with cancer. Bright, tragic, candid and true, these poems are an exceptional chronicle of what it means to find the love of your life. And what it is like to have to say goodbye.
All I can do, in what remains of my brief time,
is mention, to whoever cares to listen,
that a woman once existed, who was kind
and beautiful and brave, and I will not forget
how the world was altered, beyond recognition,
when we met.'
10. The Gigantic Beard That Was Evil by Stephen Collins
'On the buttoned-down island of Here, all is well. By which we mean: orderly, neat, contained and, moreover, beardless.
Or at least it is until one famous day, when Dave, bald but for a single hair, finds himself assailed by a terrifying, unstoppable… monster*!
Where did it come from? How should the islanders deal with it? And what, most importantly, are they going to do with Dave?
The first book from a new leading light of UK comics, The Gigantic Beard That Was Evil is an off-beat fable worthy of Roald Dahl. It is about life, death and the meaning of beards.
(*We mean a gigantic beard, basically.)'


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Our clients are indeed one of a kind! Together we raised $500 for Kidz Harbor, giving children in our area the opportunity for a better future. We want to thank you all again for your endless support and commitment to our community!
Thank you very much to Sharon Beard for swinging by our office to pick up the donation! We appreciate all that you and your incredible organization do for our local community.


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The Play Company present
I took in this fluidness around me and decided to create art that reflected it. I began imagining art that not only existed to disappear, but art that never existed in the tangible world at all. I know what you are thinking. But stop and think of the possibilities.
In this irreverent new genre-bending piece, theatre makers Christopher Chen and Lee Sunday Evans apply their playful imaginations to the work of a Chinese dissident artist. Their hybrid work invites you to navigate a labyrinthine trail between truth and perception, authority and authenticity, illusionary art and real jeopardy.
Photo Courtesy of Liu Bolin and Klein Sun Gallery
"Christopher Chen's intricately constructed, unrelentingly destabilizing puzzle of a play about the anatomy of truth and the provocative power of illusion"
~Laura Collins-Hughes, The New York Times
Louis Ozawa Changchien
Leslie Fray
Murphy Guyer
Jennifer Lim
Set: Arnulfo Maldonado
Costume: Junghyun Georgia Lee
Light: Barbara Samuels
Sound: Jeremy S. Bloom
Stage Manager:
Megan Schwarz Dickert
Production Manager:
Ian Paul Guzzone


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Police identify man found dead in downtown Nashville
Ariana Sawyer and Natalie Neysa Alund, The Tennessean
A man found dead inside a car parked in a lot on Sixth Avenue South and Mulberry Street downtown Wednesday night has been identified as Jason McGill, Metro police said Thursday.
McGill of Murfreesboro was found in his Audi sedan on Sixth Avenue South, police said.
"He appears to have died from a gunshot wound to the head," Metro police spokesman Don Aaron said.
According to Aaron, an employee of the nearby business whose vehicle was parked next to the victim's saw McGill slumped over and notified police Wednesday evening.
Police are still investigating a motive in the killing.
Check back for updates.
Reach Ariana Sawyer at 615-815-5933 or on Twitter @a_maia_sawyer.


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FILA North America's "Royalty" Pack Release Celebrates the Lineage Of Two Iconic Silhouettes
(FILA North America) - FILA's SS2016 season is in full swing and the upcoming "Royalty" pack will be a crowning moment for the Heritage category. On the heels of the January "Under The Lights"...
Feb 01, 2016 - Americas-Latest News
New Volcanic Pack by FILA USA Features the MB and Mindbender
(FILA USA) -- In addition to the already much talked about MB, FILA walks the Mindbender out of the vault for the first time since its debut in 1994. This month alone - FILA has seen tremendous...
Apr 27, 2015 - Americas-Latest News
FILA Brings Back An On Court Classic With The Return Of The MB
(FILA USA) –FILA RETROS ANOTHER ON COURT CLASSIC WITH THE RETURN OF THE MBTake a look at the FILA "Turning Tide" pack(New York, NY) - FILA's lineage in the basketball world is extensive, to say...
Apr 06, 2015 - Latest News
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Contacts information which You will receive: Regular address / /
In love like in dreams there are no impossibilities and I hope that my dream of being absolutely in love with a compatible man will find an embodiment soon; and this love and our relationship will unfold, develop and grow, change its phases but become only stronger, mature, more intimate and harmonious with time and will last for the lifetime... I would like my future soul mate to discover my warm, loving, sweet nature and feel delight in the recognition of how wonderful and exciting I am.. :) I enjoy being affectionate with my man, showing him my devotion, surrounding him with care, hearted warmth and gentleness. I am able to be playful, humorous, romantic and passionate to bring additional colors to the relationship. Well, I am the most positive and easy-going person I know and my future partner will surely love my company. :) I am optimistic and sociable, self-confident and tolerant, I can handle myself well no matter where I am and I am able to hold the reins of the conversation under any circumstances. I never give up and I always try to solve any problem which appears in a calm, productive and flexible way. I have enough patience and understanding to find a compromise and great life stamina to be ready for life changes. I treat people the way I want to be treated, I am sincere and honest, faithful and genuine. I keep my promises and I am responsible and hard-working, my future man can always rely on me.
I help children in the orphanages. In winter I like to ski and to skate. On weekends I go to the swimming pool, which helps to remain in a good form. In summer I like to roller-blade. I enjoy camping. I can do cross stitching and beads, I like reading, getting to know something new and captivating. I enjoy cooking, especially European dishes.
Seek for age: 30 to 53
I desire to find my kindred soul and together we will be able to build the relationship based on love, trust, respect and mutual understanding. I hope that he will be decent, caring, affectionate, life-loving, reliable and confident, the one who knows what he really wants from life and respects family values.


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The Maverick
The Maverick is a newly-constructed upscale wedding venue located in Kansas City, Missouri. This contemporary space has been purpose-built to provide an ergonomic space for weddings and events. The minimalist design enables couples to tailor the space to their needs for the big day. With get-ready-suites, two event floors, and a trendy bar and dining area, The Maverick welcomes couples to celebrate their entire day in one convenient location. The modern industrial design was developed by leading local architects to create a welcoming space in an ideal location with the potential to transform to suit any unique couple's style.
Facilities and Capacity
Couples can celebrate their day with up to 250 guests for a seated meal or up to 500 for a cocktail-style event. The chic bar can be fully stocked with everything from house beers to an indulgent signature cocktail. The skilled bartenders know how to create incredible infusions for every guest. Prior to the ceremony, nearly-weds can relax and prepare for the day ahead in either of the get-ready-suites. The Broadway Suite has six beauty stations complete with backlit mirrors while the Boulevard Lounge is decadently furnished and has a pool table for entertainment. The two floors of the venue allow couples and their guests a ceremony-to-reception transition as smooth as the drinks awaiting them at the bar.
Services Offered
The Maverick provides couples with a list of outside vendors to choose from to enhance their big day. Glassware for the served bar is provided onsite, as well as tables and chairs for the meal service. Setup and clean up for the event are also looked after by venue staff, allowing the couple to relax in luxury both before and after their special day. The venue is ideally located in Kansas City and has ample parking for guests arriving by car. Couples can take advantage of the opulent space for every chapter of their wedding journey, from the engagement party to the rehearsal dinner before the main event.
Frequently Asked Questions
Do you have a site fee for wedding receptions at your venue?
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What kind of settings are available?
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Real Weddings
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LAKE TAHOE, Nev. — The popular East Shoe Express shuttle service returns this summer, starting June 25. The free transit line provides beachgoers and trail users with recreational access from Incline Village, along SR28 to Sand Harbor Lake Tahoe State Park, and back.
Last year, TTD had to suspend the East Shore Express due to staff shortages following service suspensions amid the COVID-19 pandemic.
The service runs a continuous loop daily, including holidays, from the former site of the Incline Village School at 771 Southwood Boulevard to Sand Harbor State Park on State Route 28.
"The East Shore Express was one of our fastest growing routes before the pandemic and we are excited to be returning this summer," said TTD District Manager Carl Hasty. "Highway 28 is one of the most dangerous roads in Lake Tahoe and we're proud to be part of the solution to help people navigate safer."
Since its debut in 2012, the course has grown exponentially, with nearly 40,000 runners in 2019.
There are 13 partner agencies working together to implement transportation solutions and manage the SR28 corridor, a popular recreation area.
"Our primary goal is to achieve the basin's collective environmental goals and improve lake clarity by improving transportation options at Lake Tahoe and throughout the region," Hasty said. "We are currently working to provide services such as the East Shore Express which continue to ease congestion in the area during peak months."
TTD encourages all visitors to Lake Tahoe to consider leaving their car and using public transportation to better enjoy the basin safely. In coordination with the Nevada Division of State Parks and the Washoe County Regional Transportation Commission (Washoe RTC), as well as Tahoe Truckee Area Regional Transit (TART) and the Truckee North Tahoe Transportation Management Association (TNT-TMA) , TTD asks everyone to download and use Transit app to access Tahoe public transit.
Timetables and additional information for TART services can be found at TahoeTruckeeTransit.com. Additionally, passengers can download the TART Connect app for seamless curb-to-curb rides and transfers to the main bus system on the North Rim or set Google Maps settings to the public transport setting this summer and enjoy free bus services in and across the Lake Tahoe Basin.
Before planning a day trip to Sand Harbor, TTD and Nevada State Parks ask potential visitors to check the State Parks Facebook page to see if parking is available. Over the past few years, lots have filled up quickly causing traffic jams on the already congested narrow highway. For frequent updates, you can visit their Facebook page.
Next year, Nevada State Parks will add a reservation system for parking at Sand Harbor as another tool to reduce traffic on SR28.


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Warwick Applefest
Application Fee $0.00
Website www.warwickapplefest.com
Promoter Warwick Applefest
Warwick Applefest takes place in the small picturesque Upstate NY Village of Warwick New York. It features food and craft vendors, live music performances, local food and beverages, restaurants, small shops, amusement rides, games, a carnival area, and nearby agricultural operations such as orchards, wineries, resorts, and sports activities. Come for the day. Stay for a week!


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We have moved to GitHub Issues
You are viewing the read-only archive of Umbraco's issue tracker. To create new issues, please head over to GitHub Issues.
Make sure to read the blog posts announcing the move for more information.
Created by Rasmus Eeg Møller 20 May 2014, 06:39:42 Updated by Shannon Deminick 19 Jun 2014, 08:02:07
Is required for: U4-3471
Type a tag, press enter, click outside tag field, will add the last text to fake input. This is a default behaviour by typeahead.
Priority: Normal
Type: Bug
State: Fixed
Assignee: Shannon Deminick
Difficulty: Normal
Backwards Compatible: True
Fix Submitted:
Affected versions: 7.1.3
Due in version: 7.1.5
Story Points: