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In JoVE (1)
Other Publications (19)
- Antonie Van Leeuwenhoek
- Virology
- Genetics and Molecular Research : GMR
- FEMS Microbiology Reviews
- Biochemical and Biophysical Research Communications
- Nature Reviews. Microbiology
- Applied and Environmental Microbiology
- Applied and Environmental Microbiology
- PloS One
- Proceedings of the National Academy of Sciences of the United States of America
- Nature Biotechnology
- The Journal of Biological Chemistry
- Clinical Chemistry
- Journal of Bacteriology
- Glycobiology
- Analytical Biochemistry
- Applied and Environmental Microbiology
- Genes & Development
- Journal of Proteome Research
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Articles by Eric Johansen in JoVE
وهناك طريقة HPLC Lectin لإثراء هضميدات انتقائية الغليكوزيلاتي من العينات البيولوجية المعقدة
Eric Johansen1, Birgit Schilling2, Michael Lerch1, Richard K. Niles1, Haichuan Liu1, Bensheng Li2, Simon Allen1, Steven C. Hall1, H. Ewa Witkowska1, Fred E. Regnier3, Bradford W. Gibson2, Susan J. Fisher1, Penelope M. Drake1
1Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco - UCSF, 2Buck Institute for Age Research, 3Department of Chemistry, Purdue University
كانوا يعملون Lectin - مترافق الخرز بوروس عن HPLC. خدم معايير ببتيد سكري والضوابط الإيجابية والسلبية. MARS - 14 المستنفد ، كان chromatographed تربسين هضم البلازما البشرية والتدفق من خلال (FT) ، والكسور التي جمعت ملزمة لتحاليل ESI-LC-MS/MS. وقد أثرى Glycopeptides في جزء ملزمة مقارنة FT.
Other articles by Eric Johansen on PubMed
Product Development Strategies for Foods in the Era of Molecular Biotechnology
Antonie Van Leeuwenhoek. Aug, 2002 | Pubmed ID: 12369196
Breakthroughs in science and technology are accelerating development of new products that are impacting our regulatory systems. Genetically modified or bioengineering plant varieties have entered the food supply on a global basis, especially in the U.S. The U.S. Food and Drug Administration regulates on the premise of 'substantial equivalence' and has developed premarket notification procedures and voluntary labeling guidelines. Japan's Ministry of Health, Labor and Welfare regulates biotechnology products and has imposed biotechnology labeling regulations. However, the EU continues to be in a regulatory gridlock between member states and has proposed strict traceability and labeling guidelines. These requirements are currently restricting imports of bioengineered foods and are creating an international debate. In contrast to bioengineered plant varieties, to our knowledge, there are no strains of lactic acid bacterial starter cultures on the market that contain rDNA. The majority of strains have been improved via selection and mutagenesis. However, conjugation and electroporation have been used to transfer native lactococcal phage resistance plasmids to industrial strains. In addition, plasmids have been introduced to allow for selection of certain characteristics and then been eliminated by curing. The potential benefits of bioengineered foods are far reaching and are one of the most important opportunities of this century. However, bioengineered foods remain an emotional debate that is affecting world trade.
Identification of the Host Determinant of Two Prolate-headed Phages Infecting Lactococcus Lactis
Virology. Apr, 2003 | Pubmed ID: 12726722
A gene responsible for host determination was identified in two prolate-headed bacteriophages of the c2 species infecting strains of Lactococcus lactis. The identification of the host determinant gene was based on low DNA sequence homology in a specific open reading frame (ORF) between prolate-headed phages with different host ranges. When a host carrying this ORF from one phage on a plasmid was infected with another phage, we obtained phages with an altered host range at a frequency of 10(-6) to 10(-7). Sequencing of phage DNA originating from 10 independent single plaques confirmed that a genetic recombination had taken place at different positions between the ORF on the plasmid and the infecting phage. The adsorption of the recombinant phages to their bacterial hosts had also changed to match the phage origin of the ORF. Consequently, it is concluded that this ORF codes for the host range determinant.
Challenges when Transferring Technology from Lactococcus Laboratory Strains to Industrial Strains
Genetics and Molecular Research : GMR. 2003 | Pubmed ID: 12917807
Many genetically modified Lactococcus strains have been constructed in research laboratories around the world. Most of these have originated from laboratory strains and therefore there are several barriers to using them in an industrial setting. Laboratory strains are often plasmid-free and consequently Lac- and Prt-, rendering them unable to grow in milk. Many of the commonly used techniques have been optimised for laboratory strains and their application to industrial strains may require a great deal of effort. Often genetically modified organisms produced in the laboratory do not fit the published definition of 'food-grade' (Johansen, 1999, Encyclopedia of Food Microbiology, Academic Press, London, pp. 917-921) and a great deal of effort is required to eliminate undesirable DNA sequences. As a consequence, it is often necessary to recreate the strains in industrial backgrounds before the innovations described in the scientific literature can be applied to the real-world dairy industry.
The Long and Winding Road from the Research Laboratory to Industrial Applications of Lactic Acid Bacteria
FEMS Microbiology Reviews. Aug, 2005 | Pubmed ID: 15935510
Research innovations are constantly occurring in universities, research institutions and industrial research laboratories. These are reported in the scientific literature and presented to the scientific community in various congresses and symposia as well as through direct contacts and collaborations. Conversion of these research results to industrially useful innovations is, however, considerably more complex than generally appreciated. The long and winding road from the research laboratory to industrial applications will be illustrated with two recent examples from Chr. Hansen A/S: the implementation in industrial scale of a new production technology based on respiration by Lactococcus lactis and the introduction to the market of L. lactis strains constructed using recombinant DNA technology.
Characterization of Recombinant Camel Chymosin Reveals Superior Properties for the Coagulation of Bovine and Camel Milk
Biochemical and Biophysical Research Communications. Apr, 2006 | Pubmed ID: 16488399
Enzymatic milk coagulation for cheese manufacturing involves the cleavage of the scissile bond in kappa-casein by an aspartic acid protease. Bovine chymosin is the preferred enzyme, combining a strong clotting activity with a low general proteolytic activity. In the present study, we report expression and enzymatic properties of recombinant camel chymosin expressed in Aspergillus niger. Camel chymosin was shown to have different characteristics than bovine chymosin. Camel chymosin exhibits a 70% higher clotting activity for bovine milk and has only 20% of the unspecific protease activity for bovine chymosin. This results in a sevenfold higher ratio of clotting to general proteolytic activity. The enzyme is more thermostable than bovine chymosin. Kinetic analysis showed that half-saturation is achieved with less than 50% of the substrate required for bovine chymosin and turnover rates are lower. While raw camel milk cannot be clotted with bovine chymosin, a high clotting activity was found with camel chymosin.
Adaptation and Response of Bifidobacterium Animalis Subsp. Lactis to Bile: a Proteomic and Physiological Approach
Applied and Environmental Microbiology. Nov, 2007 | Pubmed ID: 17827318
Bile salts are natural detergents that facilitate the digestion and absorption of the hydrophobic components of the diet. However, their amphiphilic nature makes them very inhibitory for bacteria and strongly influences bacterial survival in the gastrointestinal tract. Adaptation to and tolerance of bile stress is therefore crucial for the persistence of bacteria in the human colonic niche. Bifidobacterium animalis subsp. lactis, a probiotic bacterium with documented health benefits, is applied largely in fermented dairy products. In this study, the effect of bile salts on proteomes of B. animalis subsp. lactis IPLA 4549 and its bile-resistant derivative B. animalis subsp. lactis 4549dOx was analyzed, leading to the identification of proteins which may represent the targets of bile salt response and adaptation in B. animalis subsp. lactis. The comparison of the wild-type and the bile-resistant strain responses allowed us to hypothesize about the resistance mechanisms acquired by the derivative resistant strain and about the bile salt response in B. animalis subsp. lactis. In addition, significant differences in the levels of metabolic end products of the bifid shunt and in the redox status of the cells were also detected, which correlate with some differences observed between the proteomes. These results indicate that adaptation and response to bile in B. animalis subsp. lactis involve several physiological mechanisms that are jointly dedicated to reduce the deleterious impact of bile on the cell's physiology.
Streptococcus Thermophilus Core Genome: Comparative Genome Hybridization Study of 47 Strains
Applied and Environmental Microbiology. Aug, 2008 | Pubmed ID: 18539806
A DNA microarray platform based on 2,200 genes from publicly available sequences was designed for Streptococcus thermophilus. We determined how single-nucleotide polymorphisms in the 65- to 75-mer oligonucleotide probe sequences affect the hybridization signals. The microarrays were then used for comparative genome hybridization (CGH) of 47 dairy S. thermophilus strains. An analysis of the exopolysaccharide genes in each strain confirmed previous findings that this class of genes is indeed highly variable. A phylogenetic tree based on the CGH data showed similar distances for most strains, indicating frequent recombination or gene transfer within S. thermophilus. By comparing genome sizes estimated from the microarrays and pulsed-field gel electrophoresis, the amount of unknown DNA in each strain was estimated. A core genome comprised of 1,271 genes detected in all 47 strains was identified. Likewise, a set of noncore genes detected in only some strains was identified. The concept of an industrial core genome is proposed. This is comprised of the genes in the core genome plus genes that are necessary in an applied industrial context.
Hemoglobin Cleavage Site-specificity of the Plasmodium Falciparum Cysteine Proteases Falcipain-2 and Falcipain-3
PloS One. 2009 | Pubmed ID: 19357776
The Plasmodium falciparum cysteine proteases falcipain-2 and falcipain-3 degrade host hemoglobin to provide free amino acids for parasite protein synthesis. Hemoglobin hydrolysis has been described as an ordered process initiated by aspartic proteases, but cysteine protease inhibitors completely block the process, suggesting that cysteine proteases can also initiate hemoglobin hydrolysis. To characterize the specific roles of falcipains, we used three approaches. First, using random P(1) - P(4) amino acid substrate libraries, falcipain-2 and falcipain-3 demonstrated strong preference for cleavage sites with Leu at the P(2) position. Second, with overlapping peptides spanning alpha and beta globin and proteolysis-dependent (18)O labeling, hydrolysis was seen at many cleavage sites. Third, with intact hemoglobin, numerous cleavage products were identified. Our results suggest that hemoglobin hydrolysis by malaria parasites is not a highly ordered process, but rather proceeds with rapid cleavage by falcipains at multiple sites. However, falcipain-2 and falcipain-3 show strong specificity for P(2) Leu in small peptide substrates, in agreement with the specificity in optimized small molecule inhibitors that was identified previously. These results are consistent with a principal role of falcipain-2 and falcipain-3 in the hydrolysis of hemoglobin by P. falciparum and with the possibility of developing small molecule inhibitors with optimized specificity as antimalarial agents.
7SK SnRNP/P-TEFb Couples Transcription Elongation with Alternative Splicing and is Essential for Vertebrate Development
Proceedings of the National Academy of Sciences of the United States of America. May, 2009 | Pubmed ID: 19416841
Eukaryotic gene expression is commonly controlled at the level of RNA polymerase II (RNAPII) pausing subsequent to transcription initiation. Transcription elongation is stimulated by the positive transcription elongation factor b (P-TEFb) kinase, which is suppressed within the 7SK small nuclear ribonucleoprotein (7SK snRNP). However, the biogenesis and functional significance of 7SK snRNP remain poorly understood. Here, we report that LARP7, BCDIN3, and the noncoding 7SK small nuclear RNA (7SK) are vital for the formation and stability of a cell stress-resistant core 7SK snRNP. Our functional studies demonstrate that 7SK snRNP is not only critical for controlling transcription elongation, but also for regulating alternative splicing of pre-mRNAs. Using a transient expression splicing assay, we find that 7SK snRNP disintegration promotes inclusion of an alternative exon via the increased occupancy of P-TEFb, Ser2-phosphorylated (Ser2-P) RNAPII, and the splicing factor SF2/ASF at the minigene. Importantly, knockdown of larp7 or bcdin3 orthologues in zebrafish embryos destabilizes 7SK and causes severe developmental defects and aberrant splicing of analyzed transcripts. These findings reveal a key role for P-TEFb in coupling transcription elongation with alternative splicing, and suggest that maintaining core 7SK snRNP is essential for vertebrate development.
Multi-site Assessment of the Precision and Reproducibility of Multiple Reaction Monitoring-based Measurements of Proteins in Plasma
Nature Biotechnology. Jul, 2009 | Pubmed ID: 19561596
Verification of candidate biomarkers relies upon specific, quantitative assays optimized for selective detection of target proteins, and is increasingly viewed as a critical step in the discovery pipeline that bridges unbiased biomarker discovery to preclinical validation. Although individual laboratories have demonstrated that multiple reaction monitoring (MRM) coupled with isotope dilution mass spectrometry can quantify candidate protein biomarkers in plasma, reproducibility and transferability of these assays between laboratories have not been demonstrated. We describe a multilaboratory study to assess reproducibility, recovery, linear dynamic range and limits of detection and quantification of multiplexed, MRM-based assays, conducted by NCI-CPTAC. Using common materials and standardized protocols, we demonstrate that these assays can be highly reproducible within and across laboratories and instrument platforms, and are sensitive to low mug/ml protein concentrations in unfractionated plasma. We provide data and benchmarks against which individual laboratories can compare their performance and evaluate new technologies for biomarker verification in plasma.
Identification of the GATA Factor TRPS1 As a Repressor of the Osteocalcin Promoter
The Journal of Biological Chemistry. Nov, 2009 | Pubmed ID: 19759027
A proteomic analysis of proteins bound to the osteocalcin OSE2 sequence of the mouse osteocalcin promoter identified TRPS1 as a regulator of osteocalcin transcription. Mutations in the TRPS1 gene are responsible for human tricho-rhino-phalangeal syndrome, which is characterized by skeletal and craniofacial abnormalities. TRPS1 has been shown to bind regulatory promoter sequences containing GATA consensus binding sites and to repress transcription of genes involved in chondrocyte differentiation. Here we show that TRPS1 can directly bind the osteocalcin promoter in the presence or absence of Runx2. TRPS1 binds through a GATA binding sequence in the proximal promoter of the osteocalcin gene. The GATA binding site is conserved in mice, humans, and rats, although its location and orientation are not. Mutation of the mouse or human GATA binding sequence abrogates binding of TRPS1 to the osteocalcin promoter. We show that TRPS1 is expressed in osteosarcoma cells and upon induction of osteoblast differentiation in primary mouse bone marrow stromal cells and that TRPS1 regulates the expression of osteocalcin in both cell types. The expression of TRPS1 modulates mineralized bone matrix formation in differentiating osteoblast cells. These data suggest a role for TRPS1 in osteoblast differentiation, in addition to its previously described role in chondrogenesis.
Sweetening the Pot: Adding Glycosylation to the Biomarker Discovery Equation
Clinical Chemistry. Feb, 2010 | Pubmed ID: 19959616
Cancer has profound effects on gene expression, including a cell's glycosylation machinery. Thus, tumors produce glycoproteins that carry oligosaccharides with structures that are markedly different from the same protein produced by a normal cell. A single protein can have many glycosylation sites that greatly amplify the signals they generate compared with their protein backbones.
Complete Genome Sequence of Bifidobacterium Animalis Subsp. Lactis BB-12, a Widely Consumed Probiotic Strain
Journal of Bacteriology. May, 2010 | Pubmed ID: 20190051
Bifidobacterium animalis subsp. lactis BB-12 is a commercially available probiotic strain used throughout the world in a variety of functional foods and dietary supplements. The benefits of BB-12 have been documented in a number of independent clinical trials. Determination of the complete genome sequence reveals a single circular chromosome of 1,942,198 bp with 1,642 predicted protein-encoding genes, 4 rRNA operons, and 52 tRNA genes. Knowledge of this sequence will lead to insight into the specific features which give this strain its probiotic properties.
Utilizing the O-antigen Lipopolysaccharide Biosynthesis Pathway in Escherichia Coli to Interrogate the Substrate Specificities of Exogenous Glycosyltransferase Genes in a Combinatorial Approach
Glycobiology. Jun, 2010 | Pubmed ID: 20208062
In previous work, our laboratory generated novel chimeric lipopolysaccharides (LPS) in Escherichia coli transformed with a plasmid containing exogenous lipooligosaccharide synthesis genes (lsg) from Haemophilus influenzae. Analysis of these novel oligosaccharide-LPS chimeras allowed characterization of the carbohydrate structures generated by several putative glycosyltransferase genes within the lsg locus. Here, we adapted this strategy to construct a modular approach to study the synthetic properties of individual glycosyltransferases expressed alone and in combinations. To this end, a set of expression vectors containing one to four putative glycosyltransferase genes from the lsg locus, lsgC-F, were transformed into E. coli K12 (XL-1) which is defective in LPS O-antigen biosynthesis. This strategy relied on the inclusion of the H. influenzae gene product lsgG in every plasmid construct, which partially rescues the E. coli LPS biosynthesis defect by priming uridine diphosphate-undecaprenyl in the WecA-dependent O-antigen synthetic pathway with N-acetyl-glucosamine (GlcNAc). This GlcNAc-undecaprenyl then served as an acceptor substrate for further carbohydrate extension by transformed glycosyltransferases. The resultant LPS-linked chimeric glycans were isolated from their E. coli constructs and characterized by mass spectrometry, methylation analysis and enzyme-linked immunosorbent assays. These structural data allowed the specificity of various glycosyltransferases to be unambiguously assigned to individual genes. LsgF was found to transfer a galactose (Gal) to terminal GlcNAc. LsgE was found to transfer GlcNAc to Gal-GlcNAc, and both LsgF and LsgD were found to transfer Gal to GlcNAc-Gal-GlcNAc but with differing linkage specificities. This method can be generalized and readily adapted to study the substrate specificity of other putative or uncharacterized glycosyltransferases.
A Lectin Affinity Workflow Targeting Glycosite-specific, Cancer-related Carbohydrate Structures in Trypsin-digested Human Plasma
Analytical Biochemistry. Jan, 2011 | Pubmed ID: 20705048
Glycans are cell-type-specific, posttranslational protein modifications that are modulated during developmental and disease processes. As such, glycoproteins are attractive biomarker candidates. Here, we describe a mass spectrometry-based workflow that incorporates lectin affinity chromatography to enrich for proteins that carry specific glycan structures. As increases in sialylation and fucosylation are prominent among cancer-associated modifications, we focused on Sambucus nigra agglutinin (SNA) and Aleuria aurantia lectin (AAL), lectins which bind sialic acid- and fucose-containing structures, respectively. Fucosylated and sialylated glycopeptides from human lactoferrin served as positive controls, and high-mannose structures from yeast invertase served as negative controls. The standards were spiked into Multiple Affinity Removal System (MARS) 14-depleted, trypsin-digested human plasma from healthy donors. Samples were loaded onto lectin columns, separated by HPLC into flow-through and bound fractions, and treated with peptide: N-glycosidase F to remove N-linked glycans. The deglycosylated peptide fractions were interrogated by ESI HPLC-MS/MS. We identified a total of 122 human plasma glycoproteins containing 247 unique glycosites. Importantly, several of the observed glycoproteins (e.g., cadherin 5 and neutrophil gelatinase-associated lipocalin) typically circulate in plasma at low nanogram per milliliter levels. Together, these results provide mass spectrometry-based evidence of the utility of incorporating lectin-separation platforms into cancer biomarker discovery pipelines.
Screening for Antimicrobial Resistance Genes and Virulence Factors Via Genome Sequencing
Applied and Environmental Microbiology. Apr, 2011 | Pubmed ID: 21335393
Second-generation genome sequencing and alignment of the resulting reads to in silico genomes containing antimicrobial resistance and virulence factor genes were used to screen for undesirable genes in 28 strains which could be used in human nutrition. No virulence factor genes were detected, while several isolates contained antimicrobial resistance genes.
The Cyclin K/Cdk12 Complex Maintains Genomic Stability Via Regulation of Expression of DNA Damage Response Genes
Genes & Development. Oct, 2011 | Pubmed ID: 22012619
Various cyclin-dependent kinase (Cdk) complexes have been implicated in the regulation of transcription. In this study, we identified a 70-kDa Cyclin K (CycK) that binds Cdk12 and Cdk13 to form two different complexes (CycK/Cdk12 or CycK/Cdk13) in human cells. The CycK/Cdk12 complex regulates phosphorylation of Ser2 in the C-terminal domain of RNA polymerase II and expression of a small subset of human genes, as revealed in expression microarrays. Depletion of CycK/Cdk12 results in decreased expression of predominantly long genes with high numbers of exons. The most prominent group of down-regulated genes are the DNA damage response genes, including the critical regulators of genomic stability: BRCA1 (breast and ovarian cancer type 1 susceptibility protein 1), ATR (ataxia telangiectasia and Rad3-related), FANCI, and FANCD2. We show that CycK/Cdk12, rather than CycK/Cdk13, is necessary for their expression. Nuclear run-on assays and chromatin immunoprecipitations with RNA polymerase II on the BRCA1 and FANCI genes suggest a transcriptional defect in the absence of CycK/Cdk12. Consistent with these findings, cells without CycK/Cdk12 induce spontaneous DNA damage and are sensitive to a variety of DNA damage agents. We conclude that through regulation of expression of DNA damage response genes, CycK/Cdk12 protects cells from genomic instability. The essential role of CycK for organisms in vivo is further supported by the result that genetic inactivation of CycK in mice causes early embryonic lethality.
A Lectin Chromatography/mass Spectrometry Discovery Workflow Identifies Putative Biomarkers of Aggressive Breast Cancers
Journal of Proteome Research. Feb, 2012 | Pubmed ID: 22309216
We used a lectin chromatography/MS-based approach to screen conditioned medium from a panel of luminal (less aggressive) and triple negative (more aggressive) breast cancer cell lines (n = 5/subtype). The samples were fractionated using the lectins Aleuria aurantia (AAL) and Sambucus nigra agglutinin (SNA), which recognize fucose and sialic acid, respectively. The bound fractions were enzymatically N-deglycosylated and analyzed by LC-MS/MS. In total, we identified 533 glycoproteins, ~90% of which were components of the cell surface or extracellular matrix. We observed 1011 unique glycosites, 100 of which were solely detected in ≥3 triple negative lines. Statistical analyses suggested that a number of these glycosites were triple negative-specific and thus potential biomarkers for this tumor subtype. An analysis of RNAseq data revealed that approximately half of the mRNAs encoding the protein scaffolds that carried potential biomarker glycosites were upregulated in triple negative vs. luminal cell lines, and that a number of genes encoding fucosyl- or sialyltransferases were differentially expressed between the two subtypes, suggesting that alterations in glycosylation may also drive candidate identification. Notably, the glycoproteins from which these putative biomarker candidates were derived are involved in cancer-related processes. Thus, they may represent novel therapeutic targets for this aggressive tumor subtype.


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A State Divided On Gun Control: Ilion Vs. New York City
By: Rachel Polansky
Updated: February 7, 2013
New York City may only be 4 hours away, but when it comes to political issues, some say it may as well be another state. We've spoken with Remington Arms employees, officials, even gun shop owners. But tonight, we're digging deeper and talking with YOU - the people of New York.
I started my journey in Ilion. As you can see the whole village bears the name Remington; a school, a federal credit union, and of course the giant factory in the center, the largest employer in Ilion. If the gun maker was to leave...
"The biggest concern is we that live here in Ilion and how are we gonna survive if they leave?" says Howard Mowers, Ilion resident.
"We don't have anything else but Remington and hospitals and human services agencies, and that's all we really consist of. People wouldn't really have any jobs," says Phyllis Spohn, Ilion resident.
"Frankfort, Mohawk, Ilion, Little Falls, everybody.. I mean growing up, I can't even tell you how many people I know that work there, my friends, my family. So many people work in this area that I think it would probably sink numerous towns right around here," says Kathryn Fischer, Ilion resident.
The tragedy at Newtown, at Sandy Hook Elementary, if that was to have happened at Ilion High School, do you think people would think differently?
"No. No, I think people would still have their feelings on gun control," says Spohn.
"It would be terrible but it still wasn't the guns," says Mowers.
"It's the people behind the guns that kill. It's not the gun themselves that do it." says Sally Preston, Ilion resident.
When it comes to issues like gun control, the political breakdown shows that Upstate and Downstate New York have very different views. But I wanted to know what do the people think? So I traveled to New York City to find out.
"I am so for gun control in every way, shape, and form," says Terry Steiner, Manhattan resident.
"I think that in terms of security, it makes sense," says Ayanna Colon, Brooklyn resident.
"I think it's great hes trying to stop the assault weapons ya know, as far as how much ammunition to use, because that's not needed in the city," says Barbara Pires, Bronx resident.
"I agree with the governor as far as an assault rifle is concerned, ya know, you don't need that to kill a deer," says Ralph Green, Harlem resident.
"To put it in perspective for you, Ilion's only a town of 8,000, so a lot of people are worried now that this gun manufacturer is going to leave the state which would devastate the town. People say it would be a ghost town, it would be dead, destroyed, does that change you perspective at all?" asks Rachel Polansky, reporter.
"Yeah I think that, when you put it that way, it's something that shouldn't be done," says Colon.
"That's a big concern, Remington is a huge, huge company for that area," says Ryan Simmons, Brooklyn resident.
"A large manufacturer leave a small town, from an economic perspective, it would devastate it," says Tim McCue, Manhattan resident.
"It's not just the 1400 jobs, its the entire town, it's the economy, they say it would be dead, it would be a ghostown. Does that change anything for you?" asks Rachel Polansky, reporter.
"No, no it doesn't," says Pires.
"1400 jobs, yes that's very difficult, but that's not enough to justify the deaths that have occurred over the past 10-15 years," says Marie O'Brien, Manhattan resident.
"And I feel sorry for these people if they do lose their jobs. There are so many people out of work right now but worth it, I'm sorry," says Steiner.
"I think the town needs to talk to Remington and not turn it on to gun laws. Does Remington have the best thoughts about their employees? Or are they just gonna take a reactionary stance and say ya know, screw you guys, we don't like the law and we're gonna leave. So what kind of reaction is that? Is it really a problem of the law, problem of the governor, or a company that really only cares about profits?" says Victor Sanchez, Harlem resident.
Some people bring up money, others bring up the Second Amendment: Their right to bear arms. But one man says over the years, amendments can get amended.
"It's like slavery. That wasn't fun either, so you gotta get rid of it. I mean slavery was big business, but its not right. And I'm not saying that they shouldn't make guns, they should make guns, but you can't have it rampant like that; people are dying. I guess it doesn't bother you until it gets to your doorstep, until it's your children," says Otis Sallid, Harlem resident.
Are we a state divided? That's for you to decide. And what will be the fate of Remington Arms and Ilion? That's for them to decide.


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I got quite surprised after reading all these reviews. We liked this hostel as much as we liked the town of Mersing. Its a charming and comfortable place. When we arrived, Omar was not at home but we saw a sign, that we should accommodate by ourselves. So we did. It was a simple but very clean room. When we... More
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The Fastest Selling Lei--Style and Comfort All in OneThe White Flower Lei is an attractive addition to your Luau party at an affordable price. This White Flower Lei is a step above the standard, value-priced, poly lei, but still priced-right to keep your check book as happy as your Luau guests. And the classic white style of this lei will match just about any party theme.
Surprise your guests with a unique flower lei like they have never seen before. These Luau Leis make a great addition to any Hula Halloween costume. Plus, they are a great accessory for tropical theme events like Hawaiian Luaus, corporate parties, weddings, and more. |
White Wedding -
WeddingBelle, Long Beach, NY I got marry on a beach front Hotel. I wanted the guest to feel like they left NY and went to a far warm place. I was worried the Lei was going to make the wedding look cheap and cheesey, but I am glad I bought them. Everyone in the ceremony and party wore it the entire time and it shows on all the pictures, and it looks great! even my father wore it to give me away at the altar. It was a fun touch to add to the wedding, I wish you could see the pictures, even I put one one to cut the cake and it made the dress stand out even more! No regrests at all. |
Pretty In White -
Crejun, Riverside, CA I used this white lei on the table to surround a tropical salad at each place. The theme was a whitewear island party and the leis helped to carry out the theme. They were beautiful and looked very nice...unlike plastic ones. people wore them once they got to the place assignment. My guest really looked like they were in the islands! Thanks for helping me to make the luau memorable! |
Ehh.. -
Mandy, Brooklyn Not too crazy about these, If I knew I wouldn't have paid more then 50 cents for these. Not too many flowers on it, and the very thin and cheap. Probably will fall apart before the end of the party, Glad I only bought 1 to try. |
Very Lovely White Leis -
Megan M, Austin, TX We purchased these leis for a high school production set in Hawaii. We used these for the women during the wedding scene and they look fantastic. They especially stand out when paired with other colored leis for a fuller look. |
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WASHINGTON — The Pentagon on Friday grounded its fleet of F-35 fighter jets after discovering a cracked engine blade in one plane.
The problem was discovered during what the Pentagon called a routine inspection at Edwards Air Force Base, Calif., of an F-35A, the Air Force version of the sleek new plane. The Navy and the Marine Corps are buying other versions of the F-35, which is intended to replace older fighters like the Air Force F-16 and the Navy F/A-18.
All versions — a total of 51 planes — were grounded Friday pending a more in-depth evaluation of the problem discovered at Edwards. None of the planes have been fielded for combat operations; all are undergoing testing.
In a brief written statement, the Pentagon said it is too early to know the full impact of the newly discovered problem.
The F-35 is the Pentagon's most expensive weapons program at a total estimated cost of nearly $400 billion. The Pentagon envisions buying more than 2,400 F-35s, but some members of Congress are balking at the price tag.
Friday's suspension of flight operations will remain in effect until an investigation of the problem's root cause is determined.
The Pentagon said the engine in which the problem was discovered is being shipped to a Pratt & Whitney facility in Connecticut for more thorough evaluation.MORE IN This Just InCOLCHESTER Two year after Lake Champlain flooding destroyed part of it, the Colchester Causeway... Full StoryCOLCHESTER A Massachusetts developer is hoping to carry renewable electricity from northern New... Full Story -
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Sweet Jane
Wes Craven Presents Mind Ripper
The Hills Have Eyes III
Warm Summer Rain
After appearing in family movies like Planet 51 and Tooth Fairy, Dwayne "The Rock" Johnson is jumping back into action (movies). With Fast Five opening next year, Johnson's first foray into action is Faster, which sees him as a man released from prison and seeking revenge on the men that killed his brother. The script, written by screenwriting brothers Joe and Tony Gayton, was one that Johnson hand-picked for his action movie renaissance.
"We heard that he didn't just like the script, but that he loved it," Tony told ComingSoon. "He got it... he got the 70's sort of vibe. Once we heard his passion for it, we were just thrilled. I think some actors would be nervous playing a part like this because there's not a lot of dialogue. He never asked for more dialogue. The studio asked for it a few times, but Dwayne never asked. I think that's a guy who feels comfortable with his screen presence. I don't think he minded that — in fact, I think he embraced it."
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Consumer Reviews of LENNOX furnaces
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Years owned: 6
Satisfaction Rating:
"HM30 Pice of crap!"
I will never, ever purchase another Lennox product, even if the new ones are better than the very poorly thought out hm30 Complete Heat I have in my house. I bought the house in fall of 2002. The hm30 had been installed in June 95. EVERY single year I have had a failure of $500 or more with this peice of crap. Now today, I have yet another $477 bill for a simple flow pump on the heat coil, that jammed because the plastic impeller broke apart. The impeller cannot be replaced, only the entire pump. What should be a $10 impeller is permanently attached to the $375+ pump. I have had a water tank failure, another flow pump failure, copper corrosion problems, a relay failed on the control board (which of course means a WHOLE new control board, there is corrosion on every steel, metal, aluminum and die cast part in the burner area of the water tank. Yet with every failure, the local Lennox shop has the part in stock! Even the stainless steel water tank! If you have a Lennox HM30 in your house, GET RID OF IT as soon as you can. Even one of the Lennox repairmen said if it was his house, he'd get rid of it! I have a form from Lennox to file a complaint.
Years owned: 1
Satisfaction Rating:
"Lennox High Efficiency Furnace"
High efficiency furnaces in older houses are a big mistake!! My house is now full of moisture, thick ice on all the windows in winter, and mold is forming on the walls. Furnace does not take any moisture out of the house, it adds moisture in. I have heard dozens of horror stories from others too. If you want to install a High Efficiency furnace, first replace all your windows and doors, seal up all cracks, reinsulate ceiling and walls and then install an air exchanger and a dehumidifier. Then install the new high efficiency furnace. Wish I had known that before.
Years owned: 20
Satisfaction Rating:
"What to do with Canadian Govt Grant"
I have a 3600 square foot home, I have a Lennox unit that is not giving me any problems, however I have installed a $30.00 part available at home depot for igniting the gas, I installed it myself about 7 years ago and everything works correctly. We have just signed up for the Retro Grant that gives you money back for home improvements such as windows etc... I was thinking to go to an upgraded furnace and ac unit as our bills are high and will get approx $3000.00 back from govt for having an afue of 95% +
Location: Jones, MI
Years owned: 9
Satisfaction Rating:
"Lou Simmons"
Purchased a complete System, Heat Pump/Air and a propane high efficiency g26q3-75-5 furnace. I have a four zone system. Heat exchanger failed this past winter. Serviceman switched power off to furnace in mid Jan. Lennox dealer ordered replacement but wanted $510.00 for labor. Lennox warranty states free replacement for the heat exchanger but will not supply heat exchanger to home owner nor will the Lennox dealer who has my heat exchanger supply it to another licensed installer. Lennox is protecting the Lennox dealers. Never buy Lennox again. Wish I new of a unit that would be a direct replacement and get rid of the Lennox junk.
Location: Walla Walla, WA
Years owned: 9
Satisfaction Rating:
"My Worst Nightmare"
This Lennox hm30 Complete Heat system has cost me over $6,498 in repairs over the past 9 years and now it is irrepairable. I have replaced a thermistor, a pump, a valve, a thermostat, combustion blowers x2, a gas valve, an electrode, a control board, a regulator, a combustion air orifice, and a tank. It broke down while we were gone and the pipes froze and cracked costing me another $1000 in repairs. Now Lennox offers me just $1800 toward the replacement system which costs $3000. How pitiful! We have gone without heat and hot water for many days and paid for the equivalent of a new system. This is unbelievable!
Location: Princeton, NJ
Years owned: 4
Satisfaction Rating:
"Lennox Furnace"
We purchased the Lennox complete Heat about 10 years ago. While it worked fine, Lennox offered to replace it suggesting that it would go bad soon. We took them up on their offer and installed a traditional Lennox high efficiency furnace. Since that time we have a problem every year. The ignition switch, the safety switch, controller panel, ... Now the aluminum boiler has cracked. Lucky I noticed it only a few hours after it happened since water was spraying everywhere (including all over the electronics). Lennox said the boiler is warrantied but not the electronics (which were ruined by spraying water from the cracked aluminum boiler) or the installation costs of the new boiler. It turns out it will be cheaper to put in a brand new furnace than fixing the old one. My installer recommends not touching the Lennox as he knows that they are trouble.
Years owned: 4
Satisfaction Rating:
Better off with a window fan
Location: Middleville, MI
Satisfaction Rating:
"Lennox g43"
I just bought a g43 merit. 8hr removal/installation and is very quiet. No problems and no reviews of this model. I am looking forward to saving some money with this unit (90-92% efficiency) but I am a little concerned about negative feed-back of other units. I found this sight after purchase. Replaced Ruud because of 2 cracks in heat exchanger (25 y/old). I will keep up with maintainance schedule and keep you posted.
Location: Nebraska
Years owned: 10
Satisfaction Rating:
"1997 Pulse G21"
Unit was new when I bought house. g21 Pulse Furnace. Flawless until two weeks ago. Replaced flapper when installing a/c back in 2001 just as a precaution. Unit stopped heating a couple weeks back. By shutting off/on power would force it to try to refire. Sometimes would run for a few minutes, other times wouldn't even get to pulse stage. Replaced flapper again. Ran for a week. This time trying the control box. Service tech tried for a long time (on free repeat labor to boot) to find something else wrong with it because the box is so expensive and obsolete. Tested heat exchanger for leaks. Everything checks solid. Outside of the vibration typical of pulses, couldn't be happier. Just a shame that an obsolete control box may force me to prematurely retired this furance if it fails again in a few years. The rest of the furance runs well.
Location: Pittsburgh, PA
Years owned: 2
Satisfaction Rating:
"Lennox...Never again"
Had a g61 mpv furnace and a xp15 Heat pump installed in June of '07. Have had at least 12 service calls on a variety of problems. I had to have a circuit board replace 2 years in a row. It took 20 months and a Regional Tech to find out why my "Heat Pump" wasn't heating. Was told by the salesman that replacing the filter "now was a thing of the past". After 20 months this new electronic air filter needs replaced as a cost of $235...what a mistake!!!!!!!!
The opinions expressed in these reviews are the opinions of consumers that submitted reviews to FurnaceCompare.com. They do not necessarily represent the opinions of FurnaceCompare.com.


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Holiday Fun
Published On: May 31 2011 08:22:11 PM CDT
- How much do winter sports cost?
- How to throw a fun New Year's Eve party
- Entertain your children over winter break
- Help Rudolph deliver gifts
- Top holiday DVD picks
- 5 ways to celebrate family during holidays
- Which holiday character are you?
- How much do holiday lights cost?
- 10 ideas for Thanksgiving leftovers
- How to avoid overeating at holiday dinner


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The average American family's net worth dropped almost 40% between 2007 and 2010, according to a triennial study released Monday by the Federal Reserve.
The stunning drop in median net worth -- from $126,400 in 2007 to $77,300 in 2010 -- indicates that the recession wiped away 18 years of savings and investment by families.
The Fed study, called the Survey of Consumer Finances, offers details on savings, income, debt, as well as assets and investments owned by American families.
The results, though more than a year old, highlight the marked deterioration in household finances brought on by the financial crisis and ensuing recession.
Much of the drop off in net worth -- to levels not seen since 1992 -- was attributable to a sharp decline in housing values, the Fed said.
In 2007, the median homeowner had a net worth of $246,000. Three years later that number had fallen to $174,500, a loss of more than $70,000 on average.
Families who reside in the west and south, where the housing market was especially hard hit by the recession, were worse off than their peers in the rest of the country.
Making matters worse, income levels also fell during the tumultuous three-year period, with median pre-tax income falling 7.7% as earnings from capital gains all but disappeared.
The loss of income and net worth appears to have impacted savings rates, as the number of Americans who said they saved in the prior year fell from 56.4% in 2007 to 52.0% in 2010 -- the lowest level recorded since the early 1990s.
At the same time, some families were able to escape from debt, as the share of families with debt decreased slightly to 74.9% over the three-year period. Credit card use was down, and the median account balance fell 16.1%.


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Clean pet friendly room - outdated and simple, but pet friendly with nice location to walk dog to 2 school grounds, and staff are very nice re anything we need. Yes - tiles are missing, drapes are off hangers and windows don't lock (need to use nail as jam), but large dog is accommodated without issue. Location is central and... More
- Also Known As:
- Driftwood Hotel
- Driftwood Hotel Banff
- Driftwood Motel Banff
- Official Description (provided by the hotel):
- Perfect for the budget conscious traveler looking for an Inn with a cozy mountain feel. All of the Inns rooms are finished with pine furniture and accents, and offer free wireless Internet access. The Driftwood Inn's convenient location and ample amenities make it one of Banff's best values. The Driftwood Inn is a pet friendly hotel. Located only a block from the downtown area. ... more less
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- TripAdvisor is proud to partner with Hotels.com, Booking.com, Travelocity, Expedia, Cheap Tickets, HostelBookers, Venere, EasyToBook, Olotels, Priceline, Tingo and HotelsClick so you can book your Driftwood Inn reservations with confidence. We help millions of travellers each month to find the perfect hotel for both holiday and business trips, always with the best discounts and special offers.


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The nonmarking sole on this twilight blue sneaker is perfect for the weight room, basketball court, or kickboxing class. From the January 2009 issue of O, The Oprah Magazine
Related ResourcesKeep ReadingNew Year, New RulesCristina Ferrare talks about how she is going to change her eating habits in 2010. Fitness for MenOprah Radio' host Dr. Mehmet Oz talks with John Gennaro, who has developed low-key gyms for men who are new to exercise and fitness. Blackboard Wars centers on a high school tarnished by failing academic performance and stigmatized by a culture of violence. Granted unprecedented access, cameras go behind the scenes to follow education maverick Steve Barr and no-nonsense principal Dr. Marvin Thompson as they embark together on an unpredictable mission to reinvent and revive the struggling school.

 In January 2012, the New Orleans state-run Recovery School District announced the selection of charter organization Future is Now (FIN) Schools: New Orleans to operate a Type 5 Charter of John McDonogh High School starting with the 2012-13 school year. FIN CEO Steve Barr, who had previously overseen the transition of Locke High School in the Watts neighborhood of Los Angeles, hired a new staff led by principal Dr. Marvin Thompson. Together, the new John McDonogh staff lay out clear expectations for student behavior and daily attendance while working to create a safe environment for students as they foster school pride and unity.

 Throughout the series, viewers will witness the daily challenges the staff face as their students struggle with a range of emotionally demanding obstacles including homelessness, violence, drug use, and teenage pregnancy. Tensions mount as teachers struggle to earn the trust of the students amidst intense scrutiny from the community. The staff must pull together as they strive to establish the new vision for the high school and provide a better future for their students.

 Tune in for the series premiere of Blackboard Wars, Saturday, March 2nd at 9/8c, only on OWN.

 Watch a First Look now. Jambalaya with Chicken, Shrimp, and Andouille Sausage RecipeJambalaya is the Cajun version of paella or chicken with rice. This bayou favorite from the Deep South is a mainstay at church suppers, celebrations, and wakes for a good reason: jambalaya has amazing rejuvenating powers. Whether in a giant pot to feed a hundred or a skillet to feed two, hot rice mingled with spicy juicy tomatoes, vegetables, shrimp, sausage, and chicken is the meal that holds us all together. Cue the accordion...Jambalaya and a crawfish pie, and a file gumbo... son of a gun, we'll have big fun on the bayou. Advertisement
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How to Benchmark Your Gaming PC
By: Rajeev Kuruppu
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Where Will EA Go with Star Wars?
Disney and Electronic Arts just announced a multi-year licensing deal in which the gaming behemoth will have exclusive rights to make games in the Star Wars universe. Disney wont be lett...
Maybe its because electronics are so seamlessly integrated into our daily lives, but only a few people really give their PCs a second thought when it comes to maintenance. Many have neve...
In the age of belt-tightening, it makes sense to be smart about your gaming obsession and save some cash wherever you can: switch to a free-to-play MMO, try out that homemade Mountain Dew...
The war between consoles and PCs continues, the attitude coming out of Microsoft seems pretty defiant regarding the public rumblings about possible DRM on their new system. Adam Orth, cre...
Gamers have always been enjoying games that reach literary heights with storytelling. Im referring to titles like Myst that just pull you in and completely change the way you lo...
Im just telling you right now that you need to stop reading this and go sign up for the ESO beta right now! Then come back and finish this post to find out what youre in for. Ill wait
How-To Take Apart and Clean Your PC in Under 30 Minutes
Why Building a Custom Gaming PC Yourself is Not Worth the Hassle
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Chess is a narrow way. I leave all pleasure behind. It is a time without pleasure, yes.
--Jan Timman, Dutch Grandmaster
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See, I follow his [Nakamura's] games on the Internet....He's capable of losing thirty games in a row. He plays against a strong computer. He's losing games, and he's learning. Now when he sits and plays against a human, you don't have a chance...H... | Read More
I have a new Twitter account for the gaming moments of my life. Follow my life as I battle game addiction and extreme attraction to male geeks. Bwhahahaha https://twitter.com/BackyardPawn | Read More
Found this on deviantArt. | Read More
Thanks to Jen Shahade for tipping me off to this ad: | Read More
Among other devices to kill time, during the frequent calms, Long Ghost hit upon the game of chess. With a jackknife, we carved the pieces quite tastefully out of bits of wood, and our board was the middle of a chest-lid, chalked into squares, w... | Read More
I remember one particular problem I had been trying to compose for months. There came a night when I managed at last to express that particular theme. It was meant for the delectation of the expert solver. The unsophisticated might miss the ... | Read More
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I am a Reading Specialist at the Elementary level and have had the chance to teach at all levels, K - 5. I have been teaching for over 20 years and have been teaching Reading for 15 years.
My style is to motivate my students to love and appreciate the power of reading and learning.
I have a BA in History with a minor in Archaeology from the College of Wooster (Ohio). I went back to get my teaching certification at the Elementary level from the University of Akron. I have a MA in Elementary Education with a minor in Reading from Catawba College (North Carolina). I have Reading certification in Grades K - 12.
I come from a family of educators. My grandfather, father, mother, sister, two aunts, an uncle, and a couple of cousins are, or have been, educators.


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Sage advice for the class of 2012
Randy Blaser
Updated: May 16, 2012 4:27PM
So I'm sitting at my daughter's graduation at the University of Illinois, listening to the commencement address, and all I can think of is John Lennon.
The professor is talking about the oneness of humanity. She has already quoted John Donne (you know, "No man is an island"), William Blake, Einstein, Neitzche and others on how we are all connected.
Somewhere along the line, I start to lose my focus. I think it was when she was advising that sacrifice is a gift to humanity and gave, as an example, the Hindu holy men who sleep in a 5-by-5 space so someone else can have space.
That may be a wonderful thing, but after four years of college and thousands of dollars already sacrificed, I'm poised for a little bit different message.
That's when I began hearing in my mind: "I am he as you are he as you are me and we are all together, goo goo g'joob."
Is that terrible? Have I become that jaded over the years?
Seriously, what should one say at a commencement?
Mary Schmich, the Chicago Tribune's Pulitzer Prize-winning columnist, probably said it all when she advised graduates to "wear sunscreen" in her famous column that somehow became attributed to Kurt Vonnegut.
Good advice, certainly. But by now, we've trained kids pretty well to wear sunscreen.
So what else is there?
Lessons from Tennyson
On this type of occasion, I think of a quote from Alfred, Lord Tennyson's poem "Ulysses": "I will drink Life to the lees: all times I have enjoy'd/Greatly, have suffer'd greatly, both with those/That loved me, and alone."
Graduates are embarking on their life's journey. Who knows where it will take them, where it will lead. My wish is that they will enjoy all times of life greatly, as Tennyson says, even suffering greatly because that is part of life.
May they spend those times with those they love, but if they must be alone, so be it. Sometimes there is greatness in striving alone.
Tennyson advises not to waste time or our talent:
"How dull it is to pause, to make an end,/To rust unburnish'd, not to shine in use!"
He encourages us never to give up, to always carry on:
"To strive, to seek, to find, and not to yield."
I find the poem quite inspiring. I would advise you to go read it. I like the idea of not giving up, not settling in life.
And if you decide on a life of sacrifice so the rest of humanity can have more, then go out and do that, too. But give it everything you've got. Don't contribute to the Audobon Society when you've got two cars in the garage and think you've done all you can for the environment.
Because when you drink life to the lees, no matter what you wish to make of life, you will know what it means to be human.


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Great Barrier Reef
The Great Barrier Reef is Australia's greatest natural treasure, and the world's largest coral reef. This underwater wonderland stretches for 2,300 km (1,426 miles) from Bundaberg to Australia's ... Read more
Cairns Skyrail
The Skyrail Rainforest Cableway is one of the most scenic ways to travel from Cairns, climbing up above the rainforest canopy to the charming mountain town of Kuranda. Swinging out across the ... Read more
Daintree Rainforest
Gorges, tumbling rivers, pristine rainforest, and rugged mountains – the Daintree Rainforest is a pristine environment with a World Heritage listing. Stretching from the Lower Daintree south of ... Read more
Green Island
Lapped by the sea 27 km (16.5 miles) from the mainland, Green Island is one of the most popular island day-trip destinations from Cairns. A true coral cay, the island is covered in rainforest and ... Read more
Tjapukai Aboriginal Cultural Park
Experience the vibrant music, dance, legends and food of Australia's indigenous people at the Tjapukai Aboriginal Cultural Park. There's a choice of full-day and half-day tours, or you can visit ... Read more
Cairns Zoo
Meet Australia's weird and wonderful native animals at Cairns Zoo, a spectacular coastal drive from Cairns at Palm Cove. From crocodiles and alligators to koalas, dingoes and red pandas, all ... Read more
Cape Tribulation
Tropical rainforest, coral ocean and wild rivers come together at Cape Tribulation, a couple of hours' drive north of Cairns and Port Douglas. This mountainous Wet Tropics World Heritage Area ... Read more
Mossman Gorge
Beautiful Mossman Gorge is a special place for a swim on a hot day, with crystal-clear water tumbling over the rocks and boulders of the Mossman River. The gorge is also popular with bushwalkers ... Read more
Cairns Cruise Port
Cairns Cruise Port One of Australia's busiest cruise ports, Cairns may be best known for its close access to the Daintree tropical rainforest and the Great Barrier Reef. Once the site of mangrove ... Read more


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By Andrew Jonathan S. Bagaoisan
I will remember where I was when I learned we lost Dolphy.
The big story that day was the extreme traffic wrought by keeping the Metro Manila buses along one lane of EDSA. Our van was at a concrete island on the turn to Quezon Avenue from EDSA.
After we aired a live report for TV Patrol, the news desk told us to stay put while deciding if we would do another for the 11 p.m. newscast.
It was nearly 9 and raining. A crew mate and I were already settling down from dinner, shut in our crew cab.
The desk editor on duty called. "Who's on standby at Makati Med?"
I gave the name. "Okay. You get ready too," he said, and hanged up. I called our guy at Makati Medical Center.
"Nag-tweet na si Ruffa," he said. "Nag-aabangan na dito."
We read Ruffa Gutierrez's post via a workmate's Blackberry: "R.I.P Ninong Dolphy."
The Net was already abuzz, but no one was yet confirming it.
Commentators on DZMM radio were still bantering about the traffic, cryptically telling listeners who texted queries, "Please wait. We still don't know."
By then, we were told to pack up, pick up some hardware at the base, and proceed to Makati Med. Another crew watching traffic elsewhere in EDSA was diverted there too.
The TV news break greeted us when we got to ABS-CBN. Dolphy's partner, Zsa Zsa Padilla, confirmed that Dolphy had indeed passed away.
And just like that, our headlines quickly shifted gears from commuting to the loss of a showbiz great.


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Senators demand NLRB resignations
Sen. John Barrasso sounds off
- Duration 4:23
- Date Feb 3, 2013
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Sen. John Barrasso sounds off
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Appeals court recently ruled that President Obama overstepped his constitutional authority when he appoint three members to the National Labor Relations Board or NLRB.
While the senate was in recess last year Republican senator John Grasso says that means all the NLRB decision since those appointments are no good -- Earlier I asked him about his plans to block -- And National Labor Relations Board Shannon who were illegally and unconstitutionally.
Appointed by the president and made over 200 rulings.
And I want to make sure they don't make any additional rulings now the president has -- -- -- they're just gonna keep on going until the Supreme Court.
Finally -- -- that could be a year from now -- I wanna make sure there are no new rulings and no additional rulings go forward and that's the legislation I'm introducing.
And certainly no guarantee that the court will -- to decide to take up this case the Supreme Court that is.
In the meantime how does this affect business owners.
Union groups others out there who are going to be the beneficiaries or.
In some cases not be so happy about the decisions they get.
What happens to them.
Well it it absolutely creates a cloud of uncertainty.
Overall of these individuals and they're they're not gonna know whether to -- listen to or not listen to the ruins of the NLRB because what it.
One court has ruled is that those people are there illegally and unconstitutionally.
You know Shannon it's time for the president to work with congress not try to sidestep.
Congress in his responsibilities as president to work.
In the role of of just putting up -- name and in the senate has an obligation.
And an opportunity to advise and consent that's the way this ought to be done not the president saying gee I'm gonna wait and try to decide when the congress is in recess for the court ruled -- that's not up for the president to decide.
The senate gets decides when they're in session when they're not.
The -- also and the role of advise and consent is now considering the nomination former senator.
-- -- -- to become the Defense Secretary he's been grilled by some of your colleagues on the hill.
What are your greatest concerns I know you've outlined a number let's start with the Ronald.
Well in terms of Iran I think it does it Chuck Hagel is not really.
To making sure Iran does not have nuclear weapons when you take a look at some of the things that he's written.
With his.
Global zero policy eliminating some of our abilities as America maybe even to unilaterally.
I think that there that he is not really right for this job and that's where I'm coming out against him.
And of course those concerns go hand in hand with Israel.
Our ally in that region.
I he's taken a lot of tough questioning about that and -- -- former senator has said.
That he absolutely supports Israel unequivocally that would always be his position as Defense Secretary that he is explained a number of his previous comments.
Why isn't not good enough to convince you.
Well here's a long history of his time in the senate things he's written including the book that he's written since leaving the senate and when you look at his history and his record.
You have to decide do you believe all of those things are you decide.
What he is presenting just in one day in testimony and watching that testimony and -- to -- it -- -- and -- very unconvincing and it seems to me that former senator hagel was much easier when he was on the other side of the panel asking the questions and he is now -- broad enough.
On the other side having to answer them.
Defense Secretary Leon Panetta has been very clear that he's very concerned about pending defense cuts through the sequester and through other means.
He talks about the fact that it will -- be devastating those are his words are you convinced.
That senator hagel shares his concerns for that and would do what he could to protect the Pentagon and the military.
-- not convinced of that at all -- because what secretary Panetta said is what I think is the truth and previously go Chuck Hagel has said that he thought that there was so you know a lot of load didn't in the defense budget and he could take a lot of that.
-- I've just gotten back from Afghanistan visiting with our men and women in the field our troops and -- -- make sure that they have -- what they need.
During the draw down would need to make sure that there are enough troops there to continue the job that needs to be done.
And I would be very concerned if if the secretary hey golf is making recommendations.
To the president that would take out all of the troops immediately.
-- any of a busy time there on the hill with domestic and international issues senator brass at thanks for your time.
Thanks revolution and.


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News Corp. 1211 Avenue of the Americas New York, NY 10036 United States - Map Phone: 212-852-7000 Fax: 212-852-7145 Website: http://www.newscorp.com
News Corporation operates as a diversified media company worldwide. Its Cable Network Programming segment produces and licenses news, business news, sports, general entertainment, and movie programming for distribution through cable television systems and direct broadcast satellite operators primarily in the United States, Latin America, Europe, and Asia. The companys Filmed Entertainment segment produces and acquires live-action and animated motion pictures for distribution and licensing in entertainment media, as well as produces and licenses television programming worldwide. Its Television segment operates 27 broadcast television stations in the United States. The companys Direct Broadcast Satellite Television segment distributes basic and premium programming services via satellite and broadband directly to subscribers in Italy. Its Publishing segment provides newspapers and information services, such as publishing national newspapers in the United Kingdom, approximately 146 newspapers in Australia, and a metropolitan and a national newspaper in the United States; book publishing services, including the publishing of English language books worldwide; and integrated marketing services comprising the publishing of free-standing inserts, which are marketing booklets containing coupons, rebates, and other consumer offers, as well as provides in-store marketing products and services, primarily to consumer packaged goods manufacturers in the United States and Canada. The company also provides data systems and professional services that enable teachers to use data to assess student progress and deliver individualized instructions. News Corporation was founded in 1922 and is headquartered in New York, New York.


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January 12 - Eric Gordon talks after the Hornets' win over the T-Wolves
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The Ticker
KB Home rolls out energy efficient models in D.C.
Calling it home to the future, KB Home is now selling its first model ZeroHouse 2.0 home design in the Washington D.C. area to help homeowners reduce their net energy expenses.
The energy-efficient model is now being sold in the Middletown Woods community located in Waldorf, MD. Eventually, the builder will offer net-zero energy home plans at all of its communities in the greater Washington D.C. area.
KB Home says the home can save owners on 50,000 gallons of water each year and cut annual energy expenses by $6,000.
Electric bills also can be monitored and controlled from the homeowner's iPhone.


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Stayed in the off season and what a great location....right at the beach. The hotel staff was beond comparison. After 16 years of being in the hospitality industry myself, this would be the example i set to my staff should I ever own or run a hotel. Plush surroundings in the rooms, your own private patio for breakfast or wine... More
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About Us
Conference Center
Getting Here
Farm, Garden, & Watershed
Way of Tea
Workshops, Retreats, Events
Year-Long Programs
Zen Practice at Green Gulch
Daily Schedule
Guest Teachers
Central Abbot Myogen Steve Stücky
Sojun Mel Weitsman
Edward Brown
Laura Burges
Seido Lee deBarros
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Senior Dharma Teacher Zoketsu Norman Fischer
Chris Fortin
Bruce and Chris Fortin
Gary Gach
Shinchi Linda Galijan, Ph.D.
Catherine Gammon
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Alan Hawkins
Kokyo "Luminous Owl" Henkel
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Related Information
Beekeeping for Experienced Beekeepers, GGF 2/16
Alan Hawkins
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This is why I carry a gun everywhere I go.
By Doug Giles / / 1 comment
Doug Giles is the man behind ClashDaily.com. In addition to driving ClashDaily.com, Giles is a popular columnist on Townhall.com and the author of the book Raising Righteous & Rowdy Girls.
The Sandy Hook Massacre
The Sandy Hook Massacre: When Seconds Count, the Police are Minutes Away:
"Doug Giles and his team over at ClashDaily.com cut through the crap to expose what have become modern day human slaughter houses: gun free zones. Their candid arguments about allowing teachers to protect themselves and the students in their care through concealed carry should be taken seriously. As a result maybe, just maybe, we'll be able to save some lives someday rather than call the cops to report the body count." - Katie Pavlich. News Editor,Townhall.com and NYT's Best-selling Author, Fast and Furious: Barack Obama's Bloodiest Scandal and Its Shameless Cover-Up
"There were many lessons to draw from the horrific Newtown, CT school shooting. Unfortunately, most of the media and political Left came up with the wrong ones. But not Doug Giles and his writers over at ClashDaily.com. In the wake of this horror, they offered a robust defense of American citizens' right to bear arms and common sense answers to societal violence. The Sandy Hook Massacre: When Seconds Count, the Police are Minutes Away is a great resource for those interested in this vital, Constitutional issue." - S.E. Cupp, MSNBC Host and The Blaze Contributor
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Students in grades 6–8 may sign up for three sports per year, one each in the fall, winter and spring. St. Francis offers the following sports in each respective season:
More detailed information about sports and seasons is available by clicking here.
In forming teams, St. Francis uses an innovative method that helps ensure a balance between remaining competitive and giving each student playing time. Click here for details on the school's team selection process.
Click on the following links to view this season's team schedules:


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We make hiring Guitarists in Sioux City IA a snap!
Browse and book the perfect Sioux City Guitarist for your wedding, corporate event or private party on Gig Salad, the event planner's booking destination.
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Browse and compare 97 Sioux City Guitarists (and those who serve Sioux City, Iowa)
24 miles from Sioux City
Singing Guitarist / Guitarist Sioux City
Country Singer/Songwriter with over 40 years experience in music
219 miles from Sioux City
Acoustic Band / Guitarist
Greg's Music is the perfect complement to any party. Playing over 500 acoustic folk songs from artists like James Taylor, Jack Johnson, jimmy Buffet and many more. Greg will create a sing along atmosphere for any event. Greg can be seen playing the Minneapolis…
238 miles from Sioux City
Rose Music Services offers a variety of Live Music options for your wedding and/or special event. Whether you're looking for ceremony music, dinner/reception music, a dance band, or any other ensemble, Rose Music will help you create unique and personalized…
Serves Sioux City
Classical Guitarist / Guitarist
Dana Starkell's blend of talent, extensive performance experience, and unique musical skill will lend a touch of elegance to your special day. You can rest assured your event will be handled with professionalism and graced with music performed by one…
Serves Sioux City
I am a delta and hillcountry blues soloist i also use a harmonica player and djembe player if requested, presently i do blues festivals in miss. 5 this summer . All authentic delta and hillcountry blues ripping slide and trance material, Patton, Johnson,…
Serves Sioux City
Singer/Songwriter / Guitarist
Winner of the 2012 Springfield's Got Talent, a music and performance competition in Springfield, MO along with close friend Emmett Franz. Currently performing his solo act, Colin's music has the feel of a favorite album with lyrics that pull listeners…
Serves Sioux City
Country Band / Guitarist
The Mark Chapman Band formed in 2006, is a high energy alternative country group based out of SW MO. Our goal is simple; utilize outstanding musicianship, incredible song writing, and an original sound and show to the fullest extent in order to share…
Serves Sioux City
Singing Guitarist / Guitarist
Daniel Jens is the singing soldier from NBC's America's Got Talent, season 3, and founding member of the Sony Music, combat veteran singing group 4Troops. Daniel is currently available for gigs, private parties, artist development and producing.
Serves Sioux City
Banjo Player / Guitarist
5-String Banjo Player with extensive background in Bluegrass, Fiddle tunes, Jazz and New Acoustic Music.
Serves Sioux City
Jazz Guitarist / Guitarist
Dave has received the "Best of 2012" award. He has been playing guitar and performing all over the U.S., Canada, South America and Europe for various events for over 30 years. His blazing speed and pleasing tones captivates audiences of all…
Serves Sioux City
Singing Guitarist / Guitarist
Including songs by Israel Kamakawiwo'ole "Somewhere Over The Rainbow" Jason Maraz, James Taylor, Jimmy Buffet, Louis Armstrong, Cat Stevens, Howie Day, Frank Sinatra, Dylan, Bill Withers, Johnny Cash,Clapton, Sade, Neil Diamond, Bob Marley,…
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Acoustic Band / Guitarist
Mandi is a vocalist that has a great sound for most styles of music. John has been performing most of his life. He has played in numerous bands of all genres from jazz to classical, rock to pop. Together they have a repertoire that will satisfy almost…
Serves Sioux City
Singing Guitarist / Guitarist
DAVID'S BAZAAR is a veteran solo vocal-and-acoustic-guitar act, specializing in popular music from the past 60 years. Balancing older tunes with modern material, DAVID'S BAZAAR mixes instantly familiar tunes with off-the-beaten-path gems for a unique…
Serves Sioux City
One Man Band / Guitarist
Singer/12-String Guitarist Back pedal in time and hear those great 60's & 70's songs! Victor promises that not only will you be more than satisfied with his performance but you will also receive great feedback about him from your guests at your event.…
Serves Sioux City
One Man Band / Guitarist
Mister "D" ( David Simmerly ) is a one man band that provides dance music for all occasions. From Classic Rock, Blues, New Country, Old Country, Pop and Southern Rock I can entertain your party all night long. I have been singing and performing…


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Hit a home run in style when you rock this Striker tee from Jedidiah. Featuring a row of baseball bats printed on the front with the brand, this tee will have you scoring major points with the ladies. Plus, proceeds from the sale of this tee benefit StandUp For Kids, a national non-profit committed to improving the lives of homeless teenagers and at risk youth.- 100% Cotton fabric
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Over the dining room table, a field day sausage, a shareholder dinner or a yarn over the phone, shareholders know their Ravensdown team is there for them.Through our Hugh Williams scholarship we also help to support the leaders emerging from agricultural studies at Lincoln and Massey universities. We also run scholarships with Parininihi Ki Waitotara, Atihau and Mangatu Blocks Incorporation.For more information on Ravensdown-supported scholarships visit our careers website.
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9 Comments
October 18, 2010 at 3:53 pm
[...] seeing cells that can be folded up into paper airplanes! Recently revealed, MIT's paper solar cells feature five layers of solid material layered on a paper substrate. When combined, the [...]
September 9, 2010 at 3:01 pm
i think its beautiful the whole thing is greeeeeeeeeeeeeeeeeen
September 2, 2010 at 4:17 pm
[...] ready for super-charged solar cells! MIT researchers announced this week that they have developed self-assembling solar cells that can [...]
August 27, 2010 at 11:02 pm
Cost vs return on investment is important. If the house cost a million$ to build and save 200$ a month in electric bills, then it will take several times the lifetime of the building to pay off the extra $800,000 it cost to build this rotating house
August 24, 2010 at 8:19 pm
Give credit where it is due. This design looks very much like Buckminster Fuller's original Dymaxion house which rotated to follow the sun, or rotated away from the sun to keep cool. I'm sure the architect must have been inspired by Fuller. It is good to see his ideas finally getting built.
August 22, 2010 at 9:48 pm
A way to take the energy of the sun, put it to great use, and use the downfall of the amount of heat the panels capture to heat the water… Genius. =]
August 22, 2010 at 4:03 pm
Make it affordable like an average home and then it will be news. It's gorgeous, but still not available to the masses where it would have the biggest effect.
August 22, 2010 at 3:50 pm
OMG! That's so awesome. Inhabitat found yet another Photoshop fabricated "vision of the future" that is sure to save the unicorns from the evil carbon monster.
I know that outrageous headlines with no semblance of truth or reality are de rigueur on Inhabitat but, come one! People have been building real life energy positive solar homes sine the 1970′s. This is NOT a new concept. This is NOT a vision of the future. This is NOT a big deal.
August 22, 2010 at 3:38 pm
Oh wow, thats just way to cool. Nice pad!


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Diet Alternative- Proactol Plus (Ads)
| Home :: South Beach Diet |
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Moderators: Magna, Kimboroni, SBMike, RonniRoo, bethy
atlmom1155 wrote:People are going to have misconceptions and obsess about their diets and make poor choices no matter what I say, obviously, since this is something that happens repeatedly before I ever came here. It won't be my fault if someone does it again. Pop culture ideas of what "low carb" means doesn't mean we should cater to that ignorance. I think it would be better to educate people about how important it is that they maintain the diet as it is written out and not skip or reduce it any lower. Perhaps telling people that Phase 1 has "plenty" of carbs and therefore leading people to think they can skip them without harm is also dangerous.
buttbegone wrote:Sounds like eveyones been doing their research. I did a 20mi trainging run today and ended up not feeling well and light headed afterwards so I did have a brown rice english muffin. Although i cheated i still plan to P1 hopefully no more long runs until I get into P2. I really fell i need something when doing these higher miles. Any ideas for a good carb pre/post long run. Max- I use Lemon flaxseed oil. It really livens it up!
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Whale Today, Gone to Marrow
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The Layover: Singapore Pictures
Check out exclusive behind-the-scenes photos from the first episode of Anthony Bourdain's new series The Layover, premiering Monday, November 21, at 9 E/P. Tony's first stop? Singapore.
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Monisha Bruner is a news reporter with KTVO.
MACON, MO. -- Ed Walker's job is to shovel snow, and he said he gets joy from making sure others are safe.
KTVO caught up with Walker in Macon who said he's been shoveling snow since 6 a.m. Friday morning and did not finish until 6 p.m. Friday night.


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Riesenrad / 23.11.2011
The giant ferris wheel
Many major cities have high-rising architectural landmarks: Paris has the Eiffel Tower, Berlin the Fernsehturm, London the Big Ben, and Vienna the Riesenrad—a giant ferris wheel that towers over the city. Built in 1897 in honor of Emperor Franz Josef I by the English engineer Walter Basset, the Riesenrad has a dramatic history in line with its city. Open all year round, it is one of the most visited sights in Vienna. As a cultural landmark, the wheel has been featured in many films, including the city's pride and joy, The Third Man (1949).
The red cabs bring visitors high over the city—an indescribable view of Vienna and the surrounding hills of the Viennese woods is guaranteed. With 15 restored cabins, two luxury cabins and two dining cabins (though we let them stick to what they're best at: the view), the Riesenrad has cornered the market—that's because it is one of the only city landmarks that is privately owned.
The City of Vienna commissioned the renovation of the Riesenrad's square for the 2008 Football European Cup. The catastrophic result: a monstrously kitschy pseudo-historical construction and the impending insolvency of numerous subcontractors. Regardless, the Riesenrad still offers an unparalleled view of our beloved city.
source: http://vienna.unlike.at/locations/302470-Riesenrad
by: Julian&Raphi