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Stop! (Japanese pressing – with bonus tracks)
CD (Item 561994) Goontrax (Japan), 2011 — Condition: New Copy
A soulful new set from Victor Davies – maybe his best yet! Stop! finds Victor once again handling a good deal of the instrumentation himself – in a style that's more inspired by classic soul and R&B than some of his earlier work, which had a bit more folk soul touches. We loved that stuff, and we love this, too – the songs and Victor's vocals are as great as ever, with warmly percolating percussion, guitars and keys – mostly handled by Davies, with an accompanying horn section on most tunes, plus some additional assistance on drums and Rhodes on a couple. A brilliant set from one of our favorite modern soul voices on the international scene of the past decade or so! Includes "Be Your Man", "Best Thing", "Somebody" "I Want You Back", "Wasting My Time", "Words And A Song", "Stop" and more. Also includes the bonus "Somebody (Back In The Day Remix)" by Dimitri From Paris. © 1996-2013, Dusty Groove, Inc. |
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Green Bay Packers head coach Mike McCarthy announced after this morning's practice that safety Atari Bigby will be returning to North Carolina Friday to have a second surgery on his ankle. Bigby had microfracture surgery performed earlier in the spring and did his rehabilitative work on his own. In no hurry to sign his restricted free agent tender offer, the veteran stayed away from the team during organized team activities and earlier mini-camps. When he eventually signed his tender for 2010 and arrived at training camp last week, he failed his physical and was placed on the physically unable to peform list. Bigby was in North Carolina Wednesday to get another examination and the result of that will be more surgery. After practice, Bigby said it will be arthroscopic in nature to remove remaining scar tissue and he hopes it will only sideline him for about a month. He remains optimistic about starting the season on the active roster but that now looks like a real stretch. In the meantime, secondary coach Darren Perry told me today the loss of his starter will put pressure on the entire secondary group through the rest of camp. Third round draft choice Morgan Burnett has been getting nearly all the practice snaps with the number one defense alongside Pro Bowler Nick Collins. Perry feels they have adequate depth in Charlie Peprah, Will Blackmon and Derek Martin although Martin still isn't practicing because of a foot injury and Blackmon is only getting limited work early in camp since he's coming off reconstructive knee surgery from last fall. Perry said when the team was hit with injuries in the secondary last year, Martin had to step in with very little practice time with the team, as did Josh Bell later in the year, with very difficult results. He believes the team is better prepared to deal with Bigby's loss now. "It's not panic time around here", Perry told me, "but we've got to find players able to take advantage of the opportunity."
At this morning's workout under near ideal weather conditions, the Packers broke out the wet balls. Footballs dipped in large gatorade jugs were used in snapping drills with the centers and quarterabcks, along with ball security drills run by assistant coach Winston Moss. The team portion of the practice spent time on a two minute drill and the defense came up with a couple of big plays. Pat Lee intercepted Aaron Rodgers to end one drive and linebacker Robert Francois snared a deflected pass from Graham Harrell. The final posession did result in a TD toss from Rodgers.
A strong contingent of high school coaches attended practice. The Wisconsin High School Football Coaches Association Hall of Fame has a large display of enshrinees inside the Lambeau Field Atrium. Each summer before prep camps begin, coaches from all across the state are invited to watch practice and attend a luncheon at Curley's Pub.

subset: commoncrawl


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Went during the temputure being in the 100's. First room they gave us had not been cleaned. Next room had been cleaned but was not up to r standards. Soap dish dirtyand sheets and blankets old and did not smell fresh. 2nd day after being in hot temp came back & maid barely cleaned after we left a $10.00 tip.... More
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Orange-Glazed Turkey Breast
Orange marmalade and Dijon mustard combine to give a grilled Butterball Boneless Turkey Breast a unique flavor.
- Servings
- cook method
- prep
- cook
- ready
- 6
- Grill/Broil
- 10 minutes
- 1 - 2 hours
- Less than 1 1/2 hours
- no-stick cooking spray
- 1/2 cup orange marmalade
- 1 1/2 teaspoons spicy brown mustard
- 1/4 teaspoon salt
- 1 (3 pound) Butterball® Boneless Turkey Breast Roast
- Directions
- Nutrition Info
- Serving Suggestions
Spray cold grate of outdoor grill with cooking spray. Prepare grill for medium indirect heat.
Combine marmalade, mustard and salt in small bowl. Pour half of the mixture into another small bowl; set aside for serving with the cooked turkey.
Place turkey on grill grate over drip pan. Cover grill with lid. Grill 1-1/4 hours, or until meat thermometer inserted in center of thickest part of turkey reaches 170°F. Brush occasionally with the remaining glaze during the last 30 minutes of the grilling time. Carve. Serve with the heated reserved glaze.


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Chinese Taipei win mens doubles TT title
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Opposition marks Ma's 5th anniversary in office with warnings
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Reported in the press
Nothing will ever completely replace playing the game I love, however I feel like I'm starting a new adventure and I'm genuinely excited about what lies ahead. I'm fortunate to have been given many opportunities throughout my career and now I feel it's my time to give back.
David Beckham
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Intelligence- Definitely one of the most important things I look for. I'm passionate when it comes to this. If a woman cant stimulate my mind and make me think, if she cant hold an intellectual conversation with me… things are going to get dull real quick. I like a girl who is intellectually independent, I don't want to have to explain every simple thing to her, or make decisions for her because shes not smart enough to figure out what she wants. I look for a girl who can teach me things, someone I can learn from. Smart = Sexy.
Trust- This is prob the one that kills most relationships, and is why I think it's the most important to have. Trust goes both ways… I need a girl not only that I can trust, but who trusts me the same. And when I say trust, I'm not only taking about being faithful, I'm also taking about trusting my word.
Honesty - Definitely goes with trust but still needs its own section. Basically how I feel is, Dont ever lie to me… ever! Once you lie to me, and I find out, you loose all credibility. Never again will I have full confidence you are 100% truthful with me. Which in-turns results in lost of trust.
Respect - I hung out with a girl the other day who really opened my eyes when it came to this. Every time she answered one of her parent's question, she ended with a "yes sir" or "no ma'am" That right there says a lot. I'm not saying I need a girl who does that, but what Im saying is a girl who shows that much respect to her parents must give the same level of respect to a significant other. I feel a girl like that will also DEMAND that level of respect back, which is a plus cuz if you don't respect yourself, how can I expect you to respect me…
Class- I don't want a girl that's easy. If you're easy with me then you were prob easy with all the other dudes you only dated once. That shit aint cute ma. And since I'm not the type of dude just to date you to get in your pants, I'm not down with loose women. Nuff said.
Sense of Humor - pretty self-explanatory. Make me laugh, we good. Laugh at me, we good too haha ;)
Looks - Not the most important but nonetheless still important. Nobody wants to feel turned off just by looking at someone, at least I don't. I have to have some level of physical attraction, especially when getting intimate or showing affection. If I'm scared when looking at you in the morning when I wake up, it's not going to work haha
Hygiene - Thought about adding this the "Looks" section but I think its different. Looks is obvious, its how attractive i find you. Hygiene is more about the way to take care of yourself. A girl's nails, hair, make-up, scent, breath… all that, I feel a girl who doesn't take care of some as small as her hygiene, wont be too good at taking care of me.
The shit below this line doesn't really matter but deff is a plus
Cute Smile
Likes Sports
Good eye contact
Same taste in music
Doesn't smoke
Can dance
Nice ass
Freak in da bed
Does all my laundry ;) (jk)
(Source: ndye)


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Your search "fifteen minutes of fame" resulted in 1 Article
From Silverpoint to Silver Screen: Early Andy Warhol Drawings
An collection of Andy Warhol's early drawings from the 50s is currently in exhibition in the Louisiana Museum of Modern Art in Denmark.
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Introducing My Talented Friend, The Amazing Molla Mills! If you are interested in creative people, then you should pay attention to this article. I interviewed the all-rounder artist Molla Mills, a Finnish girl who will leave you open-mouthed! She has recently published her first book about crochet, VIRKKURI. From Metal Bands logos to the most exciting jewelry, Molla is always full of surprises!
Lomography & Stars Present The 'Theory of Relativity' Competition Winners Announcement! We had a blast looking through photos of all your relatives for the Lomography + Stars "Theory of Relativity" rumble! Now that they're back from their tour, we're ready to announce our winners!
StereoKino: The Mission To Make A 3D Analogue Movie Yes, you read this title correctly. And yes, this post is about what you think this title means: using two Lomokinos to produce 3D analogue movies. Or at least I tried.
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Colorado Guide and Deals
If Colorado had a magic number, it might be 14,000. The state boasts 53 snow-capped mountains––known as 'fourteeners'––whose summits reach 14,000 feet. One such monstrosity–-and a must-see on any Colorado travel guide––is Long's Peak. This fourteener punctures the cloudscape at 14,259 feet and marks the northern end of the Peak to Peak Scenic Byway, which offers a tour of... More
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Naturalists accompany participants on a zipline tour that traverses the canopies of ponderosa pines and affords majestic mountain views
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A lodge and spa surrounded by the Rocky Mountains; mountain biking and hiking close by
COURAGE. b
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With a touch of timeless European flair, Courage. b's designers fabricate all their pieces, including sweaters, jackets, and purses
Cheryl Greiner at A'La Mode Salon & Day Spa
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The licensed manicurist deftly shapes nails, gently cares for cuticles, and finishes with buffing or decorates with choice of color polish
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The rubato tag has no wiki summary.
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How does the performance of "rubato" differ in the classical and romantic styles?
In the movie Amadeus, there's a scene of a chamber orchestra where the conductor uses a large pole and pounds the floor to indicate downbeats in a strict time. And in earlier music, such as Bach ...


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The Future of Superhero Films
After the enormous success of Marvel's Cinematic Universe films (Avengers, Iron Man etc) and Nolan's Batman series, comic book movies have now proven themselves not only as a great source of revenue, but also being of genuine artistic worth. Now that the most well known superheroes have been used, has the genre already peaked?
Nolan redefined the superhero genre, showing the depth and humanity of the man in the cape and cowl. Gone was the camp crusader of the 90′s, with Batman & Robin being one of, if not the worst superhero film's of all time, most notably in one scene where Batman brandishes a Batman-branded credit card, and the declaration that he 'never leaves the Batcave without it.' The Dark Knight Rises inclusion of Catwoman eradicated Warner's abysmal outing, replacing the Wrestlemania-ready 'brought back to life by a cat' vision and instead alluded towards Bob Kane's original creation of a mysterious burglar and jewel thief. This is where DC has truly succeeded, realising the importance of character exploration. Next summer marks the return of Superman, with DC undoubtedly hoping that they can build a new critical and commercial reboot which can go one of two ways:
1) A second attempt-Hulk like flop, similarly to the Brandon Routh failed reboot just a few years ago.
2) Nolan's successful in presenting Superman in a modern context, leading to universal critical acclaim and a sizable box office performance, paving the way for future DC films.
DC will be hoping that the latter is the case, with a Justice League film in the pipeline with a 2014 release date being considered, any chance of getting the green light will almost certainly be riding on the success of Superman. One big problem with such an intended release date is bringing Superman, Batman, Wonder Woman, The Flash and Green Lantern together without the stable support of individual lead in films as Marvel achieved so well with The Avengers. Zach Snyder has said that Henry Cavill's Superman won't be part of Justice League, which will 'have it's own Superman and Batman.' With no foundations being built prior to its potential release, it's hard to see a Justice League film performing anywhere near as well as The Avengers.
With a Hulk TV series in the pipeline, could this be the next step for superheroes? Going from the big screen to the small screen allows for much more character exploration than any 2 hour film would allow. With Marvel ever-expanding its Universe, TV series could easily strengthen any future films with cameos to keep the Universe a constant.
The Avengers and the five films that lead into it—both Iron Man films, The Incredible Hulk, Captain America, and Thor have earned Marvel Entertainment almost $4 billion dollars, making it the fourth-highest-grossing film franchise of all time. With the announcement that Avengers 2 is due for a 2015 release date, and sequels to Thor, Iron Man and Captain America due prior to this, as well as a new entry based on Guardians of the Galaxy, it's hard to imagine Marvel not making a ton of money from these films. The Marvel Cinematic Universe is building an insular world that can hold any number of heroes and story arcs, and the more success it's current franchises gain, the more likely they are to transfer lesser known comic book heroes to screen. Reboots are inevitable, regardless of whether a film performs well or not, allowing for fresh vision as proven with The Amazing Spiderman. With the Superman insignia being just as recognisable as the American flag, there will always be a place for reboots whether it be on TV or the big screen, it's just a matter of when.


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Beyblade Official Handbook
Does the sound of a spinning top make you cheer? Do you dream of Beyblade battles in your sleep? Is your Beyblade collection the most organized thing in your bedroom?
If you answered "yes" to any of these questions, then you've got the true heart of a Beyblade Blader. We've got the perfect book for you.
In the Beyblade Official Handbook, you'll meet the heroes and rivals of Beyblade. You'll get info on your favorite Beys — and maybe even meet some you didn't know existed. You'll also get advice on how to become a master competitor, just like Japan's number one Blader: Gingka Hagane.
So what are you waiting for? Check out this sneak peek, and you'll be ready to let it rip!


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Sir Paul McCartney rocks Austin
Photos from Paul McCartney's debut performance at the Frank Erwin Center in Austin, Texas.
Who won "Dancing With the Stars" Tuesday night?
This season's champ was crowned last night on ABC and WSB-TV. Here's a hint, it wasn't the dancer with the highest score this week.
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5/24 7:00 p.m. – RECESS at Draft Global @ 333 West Church Street Orlando, Florida 32801
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5/29 1:00 p.m. – McDonald's Van Hit @ 1028 Lee Road Orlando FL
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Kerry makes sub-Saharan Africa visit
ADDIS ABABA, Ethiopia (AP) — Making his first official trip to sub-Saharan Africa, U.S. Secretary of State John Kerry on Saturday demanded that Nigeria respect human rights as it cracks down on Islamist extremists and pledged to work hard in the coming months to ease tensions between Sudan and South Sudan.
French help Niger to kill last jihadist bombers
NIAMEY, Niger (AP) — With the help of French special forces, Niger's military on Friday killed the last two jihadists holed up inside a dormitory on the grounds of a military garrison in the desert town of Agadez, and freed at least two soldiers who had been held hostage by the extremists, according to French and Nigerien officials.
Uganda president ousts army boss amid dispute
KAMPALA, Uganda (AP) — Ugandan President Yoweri Museveni fired his top military commander Friday, the ouster apparently linked to turmoil over Museveni's alleged plan to have his son succeed him as head of state.
Niger defense minister: Hostages freed in Agadez
NIAMEY, Niger (AP) — Hours after denying that jihadists in the city of Agadez had taken several soldiers hostage, Niger's minister of defense corrected the record in an interview with The Associated Press, saying that in fact two hostages had been found and have now been released.


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THIS FRIDAY: Filmgate Kick~off featuring Kodiak Fur (Live) + Tusk @ The Vagabond – 02/01/13
Tuesday January 29, 2013 | 10:37 am
The Vagabond Presents a FILMGATE Kick-Off Party Feburary 1st featuring the highly anticipated Miami debut of KODIAK FUR (Live) with a special opening set by TUSK. For More information, click here. Hope to see you guys there! Read More


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Volume 40 No. 19 Your hometow^n newspaper Thursday May 11,2000 © 2000 Press-Republican Newspapers Offie's Tap to close because of technicality By Shelby Becker For the Farmside-Press Located a stones' throw from the Huntley Village Hall is an establishment by the name of Offie's Tap. Offie's has been a familiar face for nearly five decades to longtime Huntley residents, but that familiar face may disappear in the near future. Owned and operated by the Offenheiser family since 1955, Offie's is home to local resi¬ dents as well as to a faithful following of patrons whom Caroline Offenheiser calls "family." Third generation and daughter of the owner, Marlene Offenheiser, "Caroline works along side her mother in the family-owned business. The clientele range from regulars who knew the founder to those with a fondness for road bikes. It is not unusual to see an impressive lineup of Harley Davidsons, Hondas and choppers of all varieties out¬ side of Offie's Tap on any given day. Nor is it unusual to see those same bikes vying for parking spaces when the church across the street holds a service - thus the problem. Established in 1852, the First Congregational Church of Huntley has maintained a peaceful coexistence with Offie's Tap for the past 50 years. Reverend Ron Woodruff said, "Sometimes there's noise, but I have no great problem with it [the tavern]." Illinois State law, however, states that no liquor establish¬ ment can be within 500 feet of a church. The village of Huntley went one step further in 1992 by declaring that within the vil¬ lage, the local ordinance would require 200 feet of distance. Offie's Tap was then - and is now - in violation of both of those laws, though the exact distance is currently in ques¬ tion. Offie's has continued to conduct business in such close proximity due to a clause known as 'grandfathering.' Basically, this clause allows for the fact that Offie's Tap' was established before this ordi¬ nance was created and is there¬ fore exempt from it. Until recently, Offie's Tap was owned and operated by Jack and Marlene Offenheiser, sec¬ ond generation in the family business. Recent personal issues have led to the owners' decision to obtain a divorce. In the inter¬ est of protecting her livelihood, Marlene found an attorney that set in motion the transfer of Jack's stock, leaving Marlene Offenheiser as sole owner of Offie's Tap. It is that change, according to the village, that invalidates the grandfather clause. Mike Coppedge, Attorney for the village, said, "The village is legally bound not to reissue a license." According to Marlene, Mayor Charlie Becker stated that because of this change of corporate interest, the liquor Itcense for Offie's Tap would be denied. "They told me [at the Village Board meeting] that they were setting a precedent with my situation." Marlene recalls. "They would shut me down on a technicality." The issue appears to be the question of this corporate change. According to Title 11, subtitle A, Chapter 1, Section 100.100 the Illinois State Liquor Commission, a corpora¬ tion is a legal entity and it is that entity that is licensed. A change in the corporate offi¬ cers [or corporate stock] does not affect the corporations' claim to that license, and only proper notification of the changes are required. "This is my living," Offenheiser said of her tavern. "We don't have problems with anyone and we give back to the town." Offenheiser was speak¬ ing of the Barney-Manningly Scholarship Fund that Offie's Tap has sponsored for nine years. Students from the high school who may be ineligible for traditional scholarships or simply ijave limited means to attend college are given oppor¬ tunity through this fund. Offie's Tap will be holding a rally on June 3 for its support¬ ers. The State Liquor Commission will hold a hearing concerning the tap at 10:30 a.m., June 14. Huntley High School students survive prom week By Susan Bohme City Editor Prom week at Huntley High School began somber note and ended with a party. The Huntley Police Department in conjunction with the Huntley Fire Department, the McHenry County Coroners Office and Flight for Life presented a DUI accident reen¬ actment at the school last week as a grim reminder of what can happen to people who drink and drive. The emergency call went out as an accident scene involving a van and a car was unveiled on the football field Tliesday morning. Emergency crews used the jaws of life to pry the top off the car to get Sara Banks, the passenger in the car, out of the wreckage. She was then airlifted to the hospital. Her date. Rich Funk, was taken away in an ambulance. Veronica Fitzsimmons, the passenger in the van, was not so lucky. Her parents were brought in to identify, her and she was taken away by the coroner in a body bag. Matt Sopchyk the driver of the van was arrested and led away in handcuffs. "My daughter was really affected by it. She told me not to go out Friday night because so many schools were hav¬ ing prom that night," said Laraine Smith, whose daughter Alison watched the reenactment. The week ended without serious incident. School offi¬ cials said about 220 students attended a dinner and dance at the Boulder Ridge Country Club in Lake in the Hills, and crowned Prom King Kyle Williams and Prom Queen Marion Hansen. About 160 of the prom-goers attended a post prom party at the high school from midnight to 4 a!m. Saturday. Farmside-Press photo by Pat Kolle Huntiey Fire Department rescue workers free victims of a drunk driver during a reenactment of a drunk driving accident at Huntley High School last week.
Object Description
Title | The Huntley Farmside |
Date | 2000-05-11 |
Month | 05 |
Day | 11 |
Year | 2000 |
Volume | 40 |
Issue | 19 |
Decade | 2000-2009 |
Creator | The Huntley Farmside |
Coverage | Huntley, Illinois, United States |
Description | Weekly Newspaper from the Huntley Area Public Library Collection |
Subject | Newspaper Archives |
Rights | This material may be protected by U.S. Copyright Law (Title 17 U.S. Code). |
Publisher | This Collection was digitized and loaded into CONTENTdm by OCLC Preservation Service Center (Bethlehem, PA) for the Huntley Area Public Library. |
Source | Reproduction of library's print newspaper archives |


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Football is a tradition here in the south for many reasons. Some love the game because they grew up playing it; others love it because they like to analyze the schematics behind each play, and then there's the people who can't get enough of those surprise hits.
While I am guilty as charged in regards to the latter, I admit that sometimes players go overboard when attempting to tackle, particularly when they lead with their helmet. Sometimes the player doing the tackling suffers an injury, and sometimes the player being tackled suffers an injury. Besides orthopedic mishaps, one of the most common injuries sustained by a football player is a concussion. So whether you just want to know more about concussions or you have a child or loved one that plays this game, let's review what a concussion is, symptoms and treatment options.
A concussion is one of many types of TBI's (traumatic brain injuries) that can occur when someone's brain is basically shaken inside their skull. Although most football helmets offer several different types of padding, the brain can still be shaken inside the skull when a player hits the ground hard, or is hit directly in the helmet area. Symptoms of a concussion can be very subtle, but more often than not a person will experience a mild to severe headache, foggy or blurred vision, vomiting or loss of consciousness. If you are with someone who has hit their head and suspect a concussion, look for these symptoms and have them checked out by a medical doctor; remember it's always best to be safe rather than sorry.
Treatment for mild concussions is usually just to rest and take it easy. This is not as easy as it seems for most football players because of the competitive drive they have, but it is nonetheless in their best interest. A doctor may suggest an over-the-counter medication to help with headaches, as well as drinking plenty of fluids.
If you have a child or loved one who plays this fun, ever-popular sport, please remember to always be on the lookout for any adverse behavior they may exhibit after an especially hard hit. Worrying too much about potential injuries can easily take the fun out of the sport, but on the other hand, it's always good to be aware of what could happen.
For more information on concussions, the symptoms of concussions or what you or your family can do to prevent them, check out the National Institute of Neurological Disorders and Stroke at ninds.nih.gov or talk with your family doctor.
Justin Glaze is an LPN and contributing columnist for the Walker County Messenger. He can be reached at 678-988-1011 or email@example.com.


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Glamour Magazine Body Size Stereotypes Survey:
What the Glamour Magazine poll shows about the assumptions women hold
Heavy women are pegged as…
"lazy" 11 times as often as thin women; "sloppy" nine times; "undisciplined" seven times; "slow" six times as often.
While thin women are seen as…
"conceited" or "superficial" about eight times as often as heavy women; "vain" or "self-centered" four times as often; and "bitchy," "mean," or "controlling" more than twice as often.
Even the "good" labels are unfair.
An overweight woman may be five times as likely to be perceived as "giving" as a skinny one. "But it just fits into the stereotype that thin women are not that way," explains Ann Kearney-Cooke, Ph.D. "It's still putting women in a box based on their body size."
This is so interesting… and really sad. The fact that heavy women ALSO judge heavy women and thin women judge other thin women is so disheartening.
Hopefully places like Stop Hating Your Body can help change this even a little bit at a time…
(click on the image for the entire article, it is worth the read!)
body image is a huge deal to me. i have struggled with it for a long time, but now i realize that it was never my fault,...


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E-mail this story
Super Bowl 2013: What will Beyonce sing?
With pretty much every news outlet still buzzing about Beyonce's Inauguration Day lip sync, the pop diva is finally offering some clues as to what her headlining spectacle at Sunday's Super Bowl halftime show will look like.
By Gerrick D. Kennedy
February 1, 2013


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Q&A with Aaron Rose
We sat down with Aaron Rose to ask him a few questions about the making of his short film Kreuzberg which was shot entirely on an iPhone 4. Here's what he had to say.
When filming Kreuzberg, you told us that you used a tripod. How did you alter the tripod to meet your needs?
I actually didn't have to alter the tripod at all. The big problem was how to attach an iPhone to an existing tripod.
What materials did you use?
I rigged a contraption using a basic steel "T" bracket from the hardware store and attached it to the tripod using a simple wing nut. It was a pretty DIY setup, but worked well for my needs (lock shots, simple pans, etc.).
How did you attach the iPhone 4 Slider Case to your tripod?
I attached the "T" bracket to the iPhone case by punching holes in it and using nylon plugs to keep it in place.
What was your experience like filming and shooting photos with your iPhone 4?
It was actually quite fun. My biggest problem was holding the camera steady when shooting hand-held. Other than that, it worked great. A zoom mechanism would have been handy, but I quickly got around it.
Do you feel that iPhone 4 will spark an increase in DIY filmmaking? If so, how?
I would hope so. Again, I don't really think a camera makes a good film. The person looking through it has to have good ideas. That said though, being able to just pull an iPhone camera out of your pocket with a few seconds notice was a really nice plus. A lot of my favorite shots in Kreuzberg were taken that way. I would see something and within seconds be shooting. That's hard to do with even the simplest SLR or video camera.
What kinds of projects would you like to see other people create using iPhone 4?
The phone/camera hybrid offers an amazing intimacy when shooting. Subjects aren't intimidated by a camera on a phone. It somehow seems less serious. This is really helpful in getting subjects to relax. I would hope that people would continue to explore this intimacy and make very personal films on the iPhone 4 that couldn't be obtained any other way.
And now, a few questions for you…
- Do you find that you take more photos and videos with your iPhone 4 than with your camera?
- How has the iPhone 4 changed the way you make and edit video?
- Has there ever been an unexpected situation when your iPhone 4 came in handy?
Leave us a comment with your answers. If you leave one of our favorite comments, we'll send you an iPhone 4 case.
11 Responses to "Q&A with Aaron Rose"
I pretty much only take photos with my iPhone 4. I rarely have my camera with me, so the iPhone has become my camera.
I am not really a video maker or editor, so it has not changed that in any way.
The iPhone has been invaluable when I get lost, which happens frequently. I really dont know what I would do without it.
I feel that DIY is making a comeback just like Aaron. The best camera is the one you have on you and that typically is my iPhone. You cant beat HD Video recording in seconds. I also hope that movie editing also move over to the mobile world just like iMovie for iPhone.
PS – I cant lie that rigged up tripod with the Slider is amazing! I want to do that sooo bad!
I live in San Francisco, so my iPhone 4 camera comes in handy all the time.. I can't just pull out a camera to take pics of crazy people in the city or on the BART, but when I see a lady dressed completely in turqouise (hair and all) or a gentleman who's busting out a drum solo on the Bart seats, the iPhone is a much more incognito way to share the fun with my friends.
1) Not only do I take more photos and videos with my iPhone 4, I take BETTER photos and videos.
2) The iPhone 4, with it's compact size, allows me to be more 'stealth-like' with my video making. It's perfect for taking candids – because of it's small and discreet size, most people don't even know I'm taking a video! And the ability to edit right on the phone is a dream-come-true! No more waiting until I get home.
3) The handiest my iPhone 4 has been: I was shopping for a leaf blower and the store was trying to gouge the price – thanks to the speed of the iPhone 4, I was able to show them the REAL price. I guess now I need a video of me blowing leaves… -
1. The camera you use the most is the one you always have with you, so I'm definitely using my iPhone 4 more than my Canon Rebel XTi.
2. As far as changing the way I make and edit video, the iPhone 4 hasn't changed it that much yet, however I do plan on making several edits with it in the near future.
3. Animal shots are always the most sporadic to get and I've found that having my iPhone 4 around always makes these easy to capture. I also have captured many photos in my car on trips or on my bike rides around town. It's very handy to have it and definitely integrates well with online sites such as twitter and facebook.
Ever since I got the iPhone 4 I started using it more as a production tool rather than just a fun gadget. With the higher end camera I've felt more confident in taking pictures with my phone and using them on my blog and flickr.
As for the video, I've been shooting what ever I can on it. From location scouting to shooting short films(just one scene stuff). And to my surprise it's really good!
The best random video recording moment I've captured was my god daughter scoring her first goal in soccer. In 720 hd glory.
Seriously, the iPhone has been the best tool to capture stunning video and photos. I still love my 7D.
1. Not sure if I take more photos with it, but I sure use it more than my DSLR! I'm taking part in a photo blog and I have found probably at least half of my photos are iPhone shots. I also find, quite frequently, that I like them better, too. The iPhone makes me think more about the shot I'm taking and as a result, I guess, they turn out better. I love that it's always available to capture the moment.
2. I'm taking more video and sharing it more.
3. Wherever I am I just hit up Google maps for directions, local businesses, etc. I love the ease of use and integration, such as how easy it is to call something found within Google maps. But, really, there's been too many times and ways to list when the iPhone has come through in a pinch.
1. I do tend to take more photos with my phone than my camera just since it's handy
2. It has not changed the way I edit though since I always use iMovie to edit on my computer
3. There's been many situations. Mostly at parties with my friends or if something funny happens while we are hanging out
1. The iPhone is the the first phone I like useing for photos but still use my DSLR mostly I do take quite a bit of video of my kids
2. It sort of introduced me to video I'm really a stills persons but I'm increasingly interested in what the iPhone can do
3. I have the worst memory so I take a lot a reference shots stills mostly… A as landscape photographer the iPhone is great I have weather, tide times, dof calcs and all sorts of apps that really help me out. -
Yes, I have taken more photos with my iPhone 4 than with my regular camera, but sometimes
I still feel as though the pictures aren't worth saving because they are just camera phone pictures. I have to reset my thinking so I will take more with it and keep them around. Also, I am more willing to share my photos on Facebook or twitter because one can actually see a beautifully taken picture I stead if trying to figure out what I've taken a photo of.I definitely think to myself wow I can take video with the phone too, so I'll end up video recording something rather than taking a photo of it. I haven't really changed my editing style yet but I am more adapt to taking video of anything that might come in handy to add to a storyline or collage if video later.
Nothing unexpected yet, but more of people around me wishing they had their camera around to get a good picture of something and I pull out my iPhone and take a picture and send it to them without knowing what I did and hearing later of the amazing picture that was taken.
Leave us a comment with your answers. If you leave one of our favorite comments, we'll send you an iPhone 4 case. -
Thanks for the comments people. This week we will be handpicking a select few and contact you so we can send you an iPhone case.


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Bochum, 7 March 2013 - With its JiveX version 4.5, which was launched last year, VISUS not only offers an update of its tried and tested PACS architecture but also introduced an optimized system equipped with innovative functions to facilitate and streamline imaging diagnostics. Image Registration, 3D Volume Rendering and Vessel Analysis: these additional image processing options have become indispensable tools in modern diagnostics.
Previously in charge of sales in Germany Mr. Bötticher joins Klaus Kleber and Jörg Holstein as third Managing Director of the Bochum-based image management company
Long-term goals: Increased coordination of national and international sales efforts and harmonization of the sales organizations
VISUS is entering the world of apps: JiveX Mobile offers image and clinical data based on HTML 5. The solution runs on all mobile platforms and integrates seamlessly with the HIS systems designed for mobile devices.
Bochum, 14 December 2012 - The past few months have clearly shown that PACS in non-radiology departments is here to stay: JiveX Integrated Imaging (PACS-II) marks the beginning of a new era in hospital-wide image data management and has already transformed many healthcare institutions both in Germany and in Switzerland. The trend to integrate DICOM and non-DICOM data in a single system is unbroken as the stream of interested visitors at the VISUS booth at Medica and RSNA 2012 indicated.
Bochum, Germany, 05 December 2012 - In the Netherlands, hospital-wide integration of image and clinical data into the PACS is almost standard procedure. Maasstad Hospital in Rotterdam has also chosen the PACS-II approach and equipped the entire facility with JiveX Integrated Imaging by VISUS. This decision is a major step towards the paperless hospital. After the successful implementation of JiveX Integrated Imaging in 26 clinical departments in 2011, among them surgery and cardiology, the hospital management decided to purchase the VISUS system also for the radiology department.
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I used this to recognize student workers at a university. I loved the design and the size of the award. It wasn't huge, but wasn't too small either. I wished it had been a little larger, but that's not a huge deal to me. The engraving plate makes the ordering and shipping extremely fast and Successories worked to get my product to me by the date of my event!
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Asbury Park Press Looking To Close Neptune Office
Office was considered state-of-the-art newspaper facility when it opened in 1985
The Asbury Park Press is putting its Neptune office building on the market and will begin to look for a new location, the newspaper reported Thursday.
According to the newspaper's website, "The company's manufacturing facility moved to Neptune from Asbury Park in 1980, followed by its news and business office operations in 1985."
The Route 66 office was considered state-of-the-art when the newspaper moved from its much smaller facility at Press Plaza in Asbury Park. The newspaper went beyond the warehouse look that was typical of media offices, and created a high-tech, modern facility with a cafeteria, a nurse's office and a running trail.
More information can be found here: http://www.app.com/article/20130110/NJNEWS/301100032/Asbury-Park-Press-puts-Neptune-building-up-sale
Doris Cauda
7:18 pm on Thursday, January 10, 2013
Who cares? Let them move and take their bias with them. It used to be a good paper, now there's nothing in it. It's become a propaganda rag for the Democratic Party. I cancelled my subscription a long time ago.
Itchy Foot Moe
7:23 am on Friday, January 11, 2013
And ironically enough Doris, you choose to read and post here, a web paper practically run by a wing of the DNC itself, the Huffington Post, where 90% of the comments posted here are about all those "eeevil Republicans" or whichever slurring variation of the week they choose to replace that word.. Just take a gander in the comments below in this article alone.
7:45 pm on Thursday, January 10, 2013
Shame on you for putting that crap anywhere near a living creature
7:48 pm on Thursday, January 10, 2013
Newspapers might have been "state of the art" in 1985. But they and their philosophy of liberalism are as old as communism and the Berlin Wall. Congratulations to the Patch for cleaning the Press' clock!!
8:06 pm on Thursday, January 10, 2013
The Asbury Park Press was nothing really until around The late 90's early 2000 when it was probing the "House of Cards real estate corruption". Before that the "Ocean County Observer" was a small competitor but lost out to the Press. I think the Press may have bought it. Not sure. Good research and reporting went into the Corruptions of more than one scam since then by the "Press".
Jennifer cordi
8:31 pm on Thursday, January 10, 2013
Folks - they aren't closing thier doors- just moving to a new facility .... so you'll still have it to clean up after the dog - LOL
Nj Ghost
8:52 pm on Thursday, January 10, 2013
Seems there isn't a left wing rag that makes any money...You'd think that all the strange people that decided to keep the dork of darkness in for a second term would certainly be enough to keep the ass press from closing one little office.
right wing nut
10:26 pm on Thursday, January 10, 2013
you mean the magic underwear guy didnt win?
Mitt the guy who said fema was immoral?
the guy who flip flopped on wanting to be president
the guy who called out republicons who are the 47% who are too stupid to know it
you mean the party who supports vaginal probes and legit rape?
The party that supported Bush who lied about Iraq and didnt put the cost of the wars on the books leaving Black Jesus to do it?
Damn I wanted Mittens to win
now tear up your fema claims and support the orange weeper of the house
Nj Ghost
10:37 pm on Thursday, January 10, 2013
Right nut, You'll have to redirect those mussing to those who voted for mittens...Not me.
The Liberal Crusader
11:25 am on Friday, January 11, 2013
Have you actually read the Press? Nowhere is there a left wing bias. Clearly you either think every piece of media except Faux News is left wing, or you can't read.
I have spoken
6:07 am on Saturday, January 12, 2013
@right wing nut
And what were you smoking beforee you posted. You sound like a fool.
8:53 pm on Thursday, January 10, 2013
typical 'classy' comments from Ocen-Monmouth below-the-Mason-Dixon rednecks.
apparently none of you are up on your biblical teachings - you're not supposed to take joy in another's misfortune....
more 'compassionate conservatism' ill feelings
in truth, the Press - and the Observer - broke a lot of important stories (Ciba-Giegy dumping toxins in the Toms River, Ritacco on the take, etc.)
Nj Ghost
9:57 pm on Thursday, January 10, 2013
Joe, Here's another classy comment from a consevative, not a Republican, They still suck....If I were a redneck I'd be proud of it....read your history.
Mr. ?
10:08 pm on Thursday, January 10, 2013
Wow JoeS, I think biblical teachings also dont profess calling individuals like "redneck" me names! Eh Joe? And misfortune? Did we "rednecks" (plural) miss that in the article? i will agree with you on there reporting the news - Ciba Geigy and Ritacco - and keping the pulic informed but "breaking the news"? That would take investigative reporting and not just publishing it. if it was not for Jean Mickleson and Don Bennett those papers would have had little substance. Opps, and forgot Helen Fitzsimons! Another point you did present is the teachings of the Bible, and now thats real news I will always read "classy" Joe. Sorry!
William J Moss
10:16 pm on Thursday, January 10, 2013
They should really close . For the past few years the really have sucked . Look at the Beacons they destroyed a good paper there for the locals . To them it is all about the all mighty dollar.
right wing nut
10:29 pm on Thursday, January 10, 2013
Hey Bill
what do you think about the accounting in Lacey?
Thomas A. Blasi
11:17 pm on Thursday, January 10, 2013
A newspaper is a reflection of the community; it prints stories germane to the intellect or lack of intellect of the community. The Press must appeal to the downtrodden, the Fox news clientele, the low hanging fruit on the social tree; that is the only way it can make a profit in this area. If you think I am exaggerating, merely read some of the comments.
Mr. ?
12:04 am on Friday, January 11, 2013
Another Guru of the medsus intellect and purveyor of profoundness and an insult to the "Fox news Clientel" too (as in also) to boot! You would think those superior individuals would not do as we "low hanging fruiters on the social tree" do. Did Tom insult us posters or the community of Ocean and Monmouth? Where do you live Tomas A. ?
The Liberal Crusader
11:28 am on Friday, January 11, 2013
He probably insulted both, with good reason.
I have spoken
6:11 am on Saturday, January 12, 2013
The APP has been a rag for a long time....since Gannett took over. Now it's value is comparable to The Enquirer, The Star and other rags that report about 3 headed babies and I was impregnanted by an alien creature. Since Hollis Towns took the reins it got even worst. I'm seeing more reliable and honest news reporting on The Patch.
Mr. Marlboro
1:27 am on Friday, January 11, 2013
In my opinion, the "Asbury Park Press" peak some years ago and has been going downhill precipitously ever since it was purchased by Gannett. For those that don't know, Gannett also owns "The Press Journal", that's the newspaper in Westchester County, NY, that is publishing the names and addresses of LEGAL gun owners' up-there. I'm not a gun owner, member of the NRA or any of that stuff; I just think it's wrong for the newspaper to be doing it and am personally appalled by it. I am now boycotting all Gannett publications and other media, and am thinking of boycotting its advertisers and letting them know why I'm doing it. If you feel likewise, spread the word!
richard nicoletti
9:44 am on Friday, January 11, 2013
You're right, Mr. Marlboro. Those big conglomerates ruin everything they touch. I read the Press for 30 years; had to quit when it decided to go ultra-liberal.
Dame Bridgid
7:24 pm on Friday, January 25, 2013
Do not forget they publish USA Today too. They also own a lot of ABC, NBC, & CBS affiliate stations around the country.
I stopped buying the Asbury Park Press years ago when I was unable to get any news on the rare "subway series" that Autumn. In my paper they omitted details for The World Series from their sport section because I was not in that precise area of the Gannet distributorship allowed to receive it(without paying more money, of course!). The wanted me to purchase a second paper just to read that story alone. The local town news can be gotten on line now, making them obsolete.
Goodby Press dinosaur, heading for the La Brea tar pits! About time!
Dame Bridgid
7:26 pm on Friday, January 25, 2013
Sorry for the typo, that sentence should have read "They wanted me to purchase a second paper, just to read that story alone.".
6:36 am on Friday, January 11, 2013
The Asbury Park Press, to me, will always be a special paper. One always tucked under my grandfathers arm when he made the trek to visit us, was a gift for my homesick mother. My father was a serviceman which gave us chance to read a variety of local papers, but the Asbury Park Press was always our slice of home. To me it is the smell of coffee and raisin toast on a Sunday while cutting coupons. It is the wonderful memory of a grandpop that always wore a suit, hat and a smile. If it were bias, than so be it. I choose not to tarnish the memory. And like many folk, my fireplace burns, my bird cage collects and i just might add it to the use of the doggie poop for the back yard after reading the ingenious commentary here.
8:13 am on Friday, January 11, 2013
All papers are failing. Electronic media like this is to blame plus here we go...liberals.
Liberals run the papers but only conservatives are mostly news junkies and readers of them. Most young liberals or democrats will not support a print paper yet they are written for them.
8:44 am on Friday, January 11, 2013
So according to your reasoning, we have the conservatives to blame for supporting the liberal press. How about both sides are showing a preference for quicker access without having to bring in a rain soaked lump to put in the oven.
1:31 pm on Saturday, January 12, 2013
That doesn't seem fair to the Patch. I have had a couple of instances where I complained and I must say the editor of the Berkeley Patch is straight shooting and just trying very hard to cover this complex story of a storm called Sandy. She tells you up front what her political leanings are and yet most of us read the stories and jump to the comments which are written by folks who hold all sorts of views.
To me, the Patch is a breath of fresh air. The Editor is if anything more than willing to let all sorts of opinions fly around and it's a shame if we who write those comments don't understand that more than politics are involved in this very complex mess we are in. Yes, there certainly could be streamlining of the administration of the programs and towns and committees and it would help if more towns supplied the Patch with in depth information about just what the rules of these many games are.
But there is a time for specific finger pointing if you know someone is not doing his or her job but this storm was neither a gift from the democrats or the republicans and when the chips are down it is going to be up to New Jerseyans to pitch in and help any and everyone in need. The more we can do for our communities, the less we will need government help.
And Governments on all levels are flat out broke. So when you finish typing here, head for a troubled spot and donate and hour or so of your time to helping things get better. We will all be better off for it.
10:35 am on Friday, January 11, 2013
Exactly, it was actually listed as "Breaking News" last night and when I asked why this was considered breaking news, my comment was deleted.
11:01 am on Friday, January 11, 2013
Everything is breaking news on Patch. They get a little crazy around here with that tag line. Kind of annoying.
12:58 pm on Friday, January 11, 2013
AAP may be liberal but don't think you'll do better at Patch. Patch was launched by AOL which is now together with the Huffington Post.
1:38 pm on Saturday, January 12, 2013
I disagree with that. Patch seems to let all sorts of ideas through and only censors things most of us would agree are not fit for print. The Patch articles seem to be straight forward and are the product of journalism: yes, they can make mistakes but they are willing to correct them and they invite community participation via the comments section which is a whole lot larger than the space devoted to the articles printed which tend to be objective.
HuffPo also tends to let ideas float from folks from left to right but that can't be said for say The New York Times which seems to cherry pick responses from readers so that they primarily fall in line with that paper's editorial outlook. The Patch isn't doing that. Thank God.
It's time to be grateful for the few things that are working and it would help if people would think through what they are writing and not just sling mud. We have enough of that to clean up!
Thomas A. Blasi
3:20 pm on Friday, January 11, 2013
For your information the Asbury Park Press ran a story in today's issue of their own paper (Friday) on the subject. According to the article, the Press Building on Rt. 66 will be sold, the Press will then rent space in a smaller facility and also they are closing their Toms Office. Not mentioned in the article but word has it that they are encouraging employees to take an early retirement package. First the Fort, now the Press, the handwriting is on the wall. This has nothing to do with liberals or conservatives, the patch or the Huffington Post, it is a sign of the times.
Thomas A. Blasi
3:22 pm on Friday, January 11, 2013
Correction to the above posting: " . . . they are closing their Toms RIVER Office."
4:42 pm on Friday, January 11, 2013
How does this article bring up such stupid comments from you people?? Does anyone have a job, or something else to worry about.. Every friggin time I read an article then the comments it's all nasty people with nasty things to say...
Mr. Marlboro
3:48 pm on Saturday, January 12, 2013
Hey, since you are commenting, I guess you are a member of the group too!
Dame Bridgid
7:40 pm on Friday, January 25, 2013
The Asbury Park Press did little to keep my readership. I commented on the fact that they lost my custom, & why. Closing their offices indicates they have a financial difficulty. My comment is appropriately linked to the topic under discussion. Your rant, however, is not. Please feel free to exit, if we bother you as your name indicates.
7:20 pm on Friday, January 11, 2013
I enjoy reading it for the local news and happenings but more and more often I find myself going to the Patch or pretty much anywhere on the internet. The Daily Mail in London actually has better coverage of many stories here in the U.S. than we do...
1:44 pm on Saturday, January 12, 2013
It's not just the Daily Mail. Newspapers from Canada to Europe and yes, Russia, are publishing articles that we need to read. The Drudge report lists so many world newspapers in its left hand column that it makes it easy to get news from other nations but with the understanding that those papers, just like ours, tend to have their own agendas. US papers are awful when it comes to covering stories that are taking place in other countries which in fact will affect us as well.
Give Drudge's left hand column a try because it's quick and certainly helps me try to understand things from other nations' points of view. And there are liberals and conservatives in their streams of journalism but at least they try to cover more than their own backyards.
8:13 pm on Friday, January 11, 2013
It is amusing to see our Press defenders resort to name-calling because they defend an antiquated news outlet whose ideas and beliefs like their own have been relegated to the trash bin of history. Their editorials sound more like the whiny ravings of a petulent child than a responsible news outlet. Again like the Press defenders sound here. In desperation, they resort to publishing the toxic words of racists, like Eugene Robinson to boost sales. Pathetic. Maybe they can hire the racist from ESPN. It seems he's looking for a job. The Press stopped reflecting any community a long time ago. When the Charms Building was being demolished, where was the APP? Asbury Park made a deal to keep that place standing with the developer. There were a lot of qustions about the condition of the structure. Not only was the APP silent, I personally called them to see if they would cover the razing. They asked me for directions to the site! Now that's sad.
8:33 pm on Friday, January 11, 2013
Now here is a "lovely" tale of how news agencies, like the APP ignore when one of their own (BBC) has a scandal. No one seems to understand how such behavior goes unchecked. Try the word "cover-up". foxnews.com/world/2013/01/11/report-details-depths-bbc-monster-child-sex-assaults/
type writer
9:23 pm on Friday, January 11, 2013
White, Christian , married, heterosexual males with moral values and a job that believe America is great and the founding Fathers were patriots, were ridiculed and mocked every day in the Asbury Park Press. We are called racists and bigots and homophobes and neanderthals by the editors and columnists. Icancelled my subscription years ago because of their liberal bias. Good bye and good riddence.
Mr. ?
10:39 pm on Friday, January 11, 2013
Hey I'm one of those people! Your right! Maybe I will cancel my subscription too. Then when all of us "nasties" cancel, the APP can move into a storage unit!
Thomas A. Blasi
11:19 pm on Friday, January 11, 2013
Gannett, the parent corporation of the Asbury Park Press cannot be hurt even if the Press goes out of business. Gannett owns USA Today and a host of other newspapers.
Thomas A. Blasi
11:21 pm on Friday, January 11, 2013
Yesterday the fort, today the Asbury Park Press, tomorrow the race track OMG!
Thomas A. Blasi
12:06 pm on Saturday, January 12, 2013
AND CONSIDER THIS: When one goes to their favorite fast food store or wherever they purchase the Press, there are so many OTHER newspapers to choose from including the Star Ledger, USA Today etc including on line newspapers. Clearly we have a choice. Unless one works for the Press and his or her job is in danger, this should not be a problem.


text:
Antibody tools to deplete or isolate teratogenic, cardiac, and blood stem cells from hESCs
Antibody tools to deplete or isolate teratogenic, cardiac, and blood stem cells from hESCs
Funding Type:
Tools and Technologies II
Grant Number:
Funds Committed:
Disease Focus:
Blood Disorders
Stem Cell Use:
Embryonic Stem Cell
Public Abstract:
Purity is as important for cell-based therapies as it is for treatments based on small-molecule drugs or biologics. Pluripotent stem cells possess two properties: they are capable of self regeneration and they can differentiate to all different tissue types (i.e. muscle, brain, heart, etc.). Despite the promise of pluripotent stem cells as a tool for regenerative medicine, these cells cannot be directly transplanted into patients. In their undifferentiated state they harbor the potential to develop into tumors. Thus, tissue-specific stem cells as they exist in the body or as derived from pluripotent cells are the true targets of stem cell-based therapeutic research, and the cell types most likely to be used clinically. Existing protocols for the generation of these target cells involve large scale differentiation cultures of pluripotent cells that often produce a mixture of different cell types, only a small fraction of which may possess therapeutic potential. Furthermore, there remains the real danger that a small number of these cells remains undifferentiated and retains tumor-forming potential. The ideal pluripotent stem cell-based therapeutic would be a pure population of tissue specific stem cells, devoid of impurities such as undifferentiated or aberrantly-differentiated cells.
We propose to develop antibody-based tools and protocols to purify therapeutic stem cells from heterogeneous cultures. We offer two general strategies to achieve this goal. The first is to develop antibodies and protocols to identify undifferentiated tumor-forming cells and remove them from cultures. The second strategy is to develop antibodies that can identify and isolate heart stem cells, and blood-forming stem cells capable of engraftment from cultures of pluripotent stem cells. The biological underpinning of our approach is that each cell type can be identified by a signature surface marker expression profile.
Antibodies that are specific to cell surface markers can be used to identify and isolate stem cells using flow cytometry. We can detect and isolate rare tissue stem cells by using combinations of antibodies that correspond to the surface marker signature for the given tissue stem cell. We can then functionally characterize the potential of these cells for use in regenerative medicine.
Our proposal aims to speed the clinical application of therapies derived from pluripotent cell products by reducing the risk of transplanting the wrong cell type - whether it is a tumor-causing residual pluripotent cell or a cell that is not native to the site of transplant - into patients. Antibodies, which exhibit exquisitely high sensitivity and specificity to target cellular populations, are the cornerstone of our proposal. The antibodies (and other technologies and reagents) identified and generated as a result of our experiments will greatly increase the safety of pluripotent stem cell-derived cellular therapies.
Statement of Benefit to California:
Starting with human embryonic stem cells (hESC), which are capable of generating all cell types in the body, we aim to identify and isolate two tissue-specific stem cells – those that can make the heart and the blood – and remove cells that could cause tumors. Heart disease remains the leading cause of mortality and morbidity in the West. In California, 70,000 people die annually from cardiovascular diseases, and the cost exceeded $48 billion in 2006. Despite major advancement in treatments for patients with heart failure, which is mainly due to cellular loss upon myocardial injury, the mortality rate remains high. Similarly, diseases of the blood-forming system, e.g. leukemias, remain a major health problem in our state.
hESC and induced pluripotent stem cells (collectively, pluripotent stem cells, or PSC) could provide an attractive therapeutic option to treat patients with damaged or defective organs. PCS can differentiate into, and may represent a major source of regenerating, cells for these organs. However, the major issues that delay the clinical translation of PSC derivatives include lack of purification technologies for heart- or blood-specific stem cells from PSC cultures and persistence of pluripotent cells that develop into teratomas. We propose to develop reagents that can prospectively identify and isolate heart and blood stem cells, and to test their functional benefit upon engraftment in mice. We will develop reagents to identify and remove residual PSC, which give rise to teratomas. These reagents will enable us to purify patient-specific stem cells, which lack cancer-initiating potential, to replenish defective or damaged tissue.
The reagents generated in these studies can be patented forming an intellectual property portfolio shared by the state and the institutions where the research is carried out. The funds generated from the licensing of these technologies will provide revenue for the state, will help increase hiring of faculty and staff (many of whom will bring in other, out-of-state funds to support their research) and could be used to ameliorate the costs of clinical trials – the final step in translation of basic science research to clinical use. Only California businesses are likely to be able to license these reagents and to develop them into diagnostic and therapeutic entities; such businesses are at the heart of the CIRM strategy to enhance the California economy. Most importantly, this research will set the platform for future stem cell-based therapies. Because tissue stem cells are capable of lifelong self-renewal, stem cell therapies have the potential to be a single, curative treatment. Such therapies will address chronic diseases with no cure that cause considerable disability, leading to substantial medical expense. We expect that California hospitals and health care entities will be first in line for trials and therapies. Thus, California will benefit economically and it will help advance novel medical care.
Progress Report:
Year 1
Our program is focused on improving methods that can be used to purify stem cells so that they can be used safely and effectively for therapy. A significant limitation in translating laboratory discoveries into clinical practice remains our inability to separate specific stem cells that generate one type of desired tissue from a mixture of 'pluripotent' stem cells, which generate various types of tissue. An ideal transplant would then consist of only tissue-specific stem cells that retain a robust regenerative potential. Pluripotent cells, on the other hand, pose the risk, when transplanted, of generating normal tissue in the wrong location, abnormal tissue, or cancer. Thus, we have concentrated our efforts to devise strategies to either make pluripotent cells develop into desired tissue-specific stem cells or to separate these desired cells from a mixture of many types of cells. Our approach to separating tissue-specific stem cells from mixed cultures is based on the theory that every type of cell has a very specific set of molecules on its surface that can act as a signature. Once this signature is known, antibodies (molecules that specifically bind to these surface markers) can be used to tag all the cells of a desired type and remove them from a mixed population. To improve stem cell therapy, our aim is to identify the signature markers on: (1) the stem cells that are pluripotent or especially likely to generate tumors; and (2) the tissue-specific stem cells. By then developing antibodies to the pluripotent or tumor-causing cells, we can exclude them from a group of cells to be transplanted. By developing antibodies to the tissue-specific stem cells, we can remove them from a mixture to select them for transplantation. For the second approach, we are particularly interested in targeting stem cells that develop into heart (cardiac) tissue and cells that develop into mature blood cells. As we develop ways to isolate the desired cells, we test them by transplanting them into animals and observing how they grow. Thus, the first goal of our program is to develop tools to isolate pluripotent stem cells, especially those that can generate tumors in transplant recipients. To this end, we tested an antibody aimed at a pluripotent cell marker (stage-specific embryonic antigen-5 [SSEA-5]) that we previously identified. We transplanted into animals a population of stem cells that either had the SSEA-5-expressing cells removed or did not have them removed. The animals that received the transplants lacking the SSEA-5-expressing cells developed smaller and fewer teratomas (tumors consisting of an abnormal mixture of many tissues). Approaching the problem from another angle, we analyzed teratomas in animals that had received stem cell transplants. We found SSEA-5 on a small group of cells we believe to be responsible for generating the entire tumor. The second goal of the program is to develop methods to selectively culture cardiac stem cells or isolate them from mixed cultures. Thus, in the last year we tested procedures for culturing pluripotent stem cells under conditions that cause them to develop into cardiac stem cells. We also tested a combination of four markers that we hypothesized would tag cardiac stem cells for separation. When these cells were grown under the proper conditions, they began to 'beat' and had electrical activity similar to that seen in normal heart cells. When we transplanted the cells with the four markers into mice with normal or damaged hearts, they seemed to develop into mature heart cells. However, these (human) cells did not integrate with the native (mouse) heart cells, perhaps because of the species difference. So we varied the approach and transplanted the human heart stem cells into human heart tissue that had been previously implanted in mice. In this case, we found some evidence that the transplanted cells differentiated into mature heart cells and integrated with the surrounding human cells. The third goal of our project is to culture stem cells that give rise only to blood cells and test them for transplantation. In the past year, we developed a new procedure for treating cultures of pluripotent stem cells so that they differentiate into specific stem cells that generate blood cells and blood vessels. We are now working to refine our understanding and methods so that we end up with a culture of differentiated stem cells that generates only blood cells and not vessels. In summary, we have discovered markers and tested combinations of antibodies for these markers that may select unwanted cells for removal or wanted cells for inclusion in stem cell transplants. We have also begun to develop techniques for taking a group of stem cells that can generate many tissue types, and growing them under conditions that cause them to develop into tissue-specific stem cells that can be used safely for transplantation.Publications
- Chem Commun (Camb) (2013) Synthesis of a photocaged tamoxifen for light-dependent activation of Cre-ER recombinase-driven gene modification. (PubMed: 23612712)
- Nat Biotechnol (2012) Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. (PubMed: 22634564)


text:
Fatal parasail accidents renew calls for rules
MIAMI BEACH, Fla. (AP) — Soaring high above the ocean, tethered to a boat, a parasail ride is at once exhilarating and peaceful, even quiet. But every year, there are accidents.
The Parasail Safety Council, which tracks injuries and deaths nationwide, reports more than 70 people have been killed and at least 1,600 injured between 1982 and 2012, out of an estimated 150 million parasail rides during those 30 years.
That's a casualty rate of about one per 90,000 rides. In comparison, the chance of being seriously injured at an amusement park is about one in 9 million rides, according 2010 data from the International Association of Amusement Parks and Attractions.
Despite parasailing's inherent risk, few federal or state safety regulations exist for it. In Florida, which has by far the largest number of parasail operators at about 120, repeated efforts to enact new rules following fatal accidents have gone nowhere. Florida is seen by safety proponents as a national bellwether because of parasailing's popularity in a state highly dependent on tourist dollars.
The lack of safety regulations frustrates Shannon Kraus, mother of two girls who crashed into a Pompano Beach hotel roof in 2007 when their parasail line snapped during a storm. One of the girls, 15-year-old Amber May White, later died of her injuries, while her sister Crystal, then 16, has had a long road to recovery from head injuries.
"Nobody has listened to me from day No. 1," Kraus said. "I've just been shoved aside. I've kind of been ignored and I'm pretty angry about that."
Crystal White, now a mother of two herself and a massage therapist, said most people who sign up to parasail have little idea it's less regulated than the average carnival ride.
"They just need to know that if they go up, and something bad happens, there's nothing they can do about it, because there are no laws, or rules, or regulations," she said.
Indeed, five years after the girls' accident just off the same beach, a Connecticut woman died when her harness gave way and she fell about 150 feet into the ocean. The Aug. 15 death of 28-year-old Kathleen Miskell has prompted the first National Transportation Safety Board investigation into a parasailing fatality. Safety advocates hope Florida lawmakers will give the issue a fresh look.
Mark McCulloh, who runs the Parasail Safety Council, said in the U.S. and its territories about 620 vessels offer parasail rides to about 4 million people a year. He said only New Jersey and Virginia have relatively comprehensive regulations, while the Federal Aviation Administration and U.S. Coast Guard oversee some limited aspects of the activity. Continued...
In Florida, state Sen. Gwen Margolis, a Democrat, said the proposed regulations would include inspections of parasailing equipment, new rules restricting rides during certain weather conditions and prohibitions against parasailing near fixed objects such as power lines. The proposal would also require operators to buy insurance.
Margolis said past efforts have run into opposition by parasail operators and a general anti-regulation attitude among many lawmakers in the Republican-controlled Florida Legislature.
"When you get onto anything that's recreational, you assume that somebody's inspected it and everything's OK. And you can't assume that," she said.
Most parasail operators, however, are willing to submit to safety regulations as long as they are sensible, said Dan Breitenstein, who runs Miami Beach Parasail.
"If you have someone who has never been on a parasail boat or doesn't know the workings of it, if they're making up rules that would have little effect on what we do" it wouldn't make sense, Breitenstein said. "There's definitely things we can do."
People who sign up for Miami Beach Parasail generally pay about $75 for a 15-minute ride that can take them as high as 350 feet in the air. They sit in swing-like harnesses attached to a tow bar, which is connected by ropes to the kite-like parasail above and a winch in the boat below.
As the 33-foot "High Anxiety" boat moves across the water, the parasail passengers are slowly lifted into the air and the line is let out. After the ride, the boat is slowed gradually as the line is brought in so parasailors land back on the boat on their feet.
It all seems fairly safe, and you can't beat the view, which on one recent day featured an umbrella-dotted South Beach and, beyond, its famed Art Deco hotel and entertainment district, with the greenish-blue Atlantic Ocean below.
The lack of regulation, however, means no one is looking over the operator's shoulder to make sure ropes damaged by sun and salt water are replaced. There are also little or no rules regarding age limits or experience for "spotters" who observe the riders.
And there's nothing beyond common sense to prevent an operator for taking people up windy weather, which many people say is the one variable that can most often lead to an accident. Continued...
Breitenstein, who has radars on his boats, said he maintains a five-mile buffer from any nearby bad weather. But he said in Florida, storms can develop quickly offshore — and sometimes operators might be tempted to take a little risk to make more money.
"Weather is our biggest, toughest thing," he said. "Taking chances, that's a bad habit to get into."
Follow Curt Anderson on Twitter: http://twitter.com/Miamicurt .
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text:
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text:
This article was originally distributed via PRWeb. PRWeb, WorldNow and this Site make no warranties or representations in connection therewith.
SOURCE: KettleX
After the publication of a physiological correlation of weight loss between aerobic training and resistance training, Lorna Kleidman, kettlebells fitness expert and three time kettlebell champion, advises those who want to lose weight to apply kettlebells to maximize aerobic training.
Bohemia, NY (PRWEB) January 24, 2013
On January 24, following the publication of a physiological correlation of weight loss between aerobic training and resistance training, Lorna Kleidman, kettlebells fitness expert and three time kettlebell champion, recommends applying kettlebells to maximize aerobic training.
A recent study published by the Journal of Applied Physiology comparing aerobic training to resistance training found that "when it comes to weight loss and weight maintenance, aerobic training comes out on top. If the goal is to increase lean body mass, however, resistance training is the way to go."
According to the Journal of Applied Physiology, "researchers found that the groups assigned to aerobic training and aerobic plus resistance training lost more weight than those doing resistance training only." In addition, the resistance-only group had gained weight because of an increase in lean body mass and "waist circumference decreased significantly in both the aerobic training and aerobic plus resistance training groups, but it remained steady in the resistance training-only group," reports the Journal of Applied Physiology.
"While requiring double the time commitment, a program of combined aerobic training and resistance training did not result in a greater loss of fat mass or body mass over aerobic training," the researchers wrote in the conclusion. "If increasing muscle mass and strength is the goal, a program including resistance training is required. However, balancing time commitments against health benefits accrued, it appears that aerobic training alone is the optimal mode of exercise for reducing fat mass and total body mass."
Lorna Kleidman, creator of the popular KettleX program, affirms the benefits of combining resistance and aerobic training. Kleidman states, "While aerobics focuses on losing weight and resistance on increasing lean muscle, kettlebells are designed to incorporate aerobic movements with a weight for body sculpting."
Kleidman's reputation as a [kettlebell exercises fitness instructor and three time kettlebells champion has been the backbone to the KettleX program's success. "Those who are seriously considering losing weight and feeling good about themselves should incorporate kettlebells into a twenty minute aerobics session and they will see results almost instantly."
Lorna Kleidman is a Three-Time World Champion and World Record holder in kettlebell sport and the most decorated kettlebell athlete in the country. She developed the innovative methods used in KettleX as a way to bring the benefits of the bells to everyone in an easy-to-use, comprehensive and fun format. Lorna has been teaching individuals and group classes for the past six years.
For the original version on PRWeb visit: http://www.prweb.com/releases/prweb2013/1/prweb10338392.htm
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