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More Insights and Less Reporting with Datorama
Join two of Lev's Datorama Solution Architects to learn the secrets behind cutting down on reporting hours, but increasing insights into your marketing data. Datorama is your one stop shop to analyze your business holistically, through seamless integrations to Google Analytics, Facebook, CRM, Marketing Cloud and more. Join us to learn more about the power of Datorama's cross-channel unification and visualizations.

subset: commoncrawl


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Holy Gainz Apparel BOX LOGO V2 Ladies Racerback Tank
Holy Gainz Apparel BOX LOGO V2 Ladies Racerback Tank
Sale Sold out
Regular price $18.98 USD
Regular priceUnit price per
Sale price
A portion per item gives back! Check "Giving Back" page for more info.
Care Instructions:
Wash garment inside out
Wash cold
Hang to dry for least amount of shrinking
Do not bleach/do not dry-clean
Do not iron directly on design uncovered
Colors may be different than reality because of different screen settings
The racerback cut looks good on any woman's shoulders.
.: 60% combed ringspun cotton, 40% polyester
.: Extra light fabric (3.9 oz/yd² (132 g/m²))
.: Slim fit
.: Runs smaller than usual
Giving Back
Each Purchase from Holy Gainz Apparel will give to childern and families in need all around the world and in local communities.


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I'm very excited to introduce the new Pink Belt Project range of ladies and girls fitness apparel.
Now all of us chicks who like to kick can celebrate the martial artist within every day – not just when we're in our uniform.
Wear it underneath your dobok/gi/work uniform/pjs, I don't MIND! Where it everywhere.
But the absolute kicker is that a percentage from every sale goes direct to my Pink Belt Scholarship fund, empowering women in need with free Taekwondo for a year.
And the full range (including youth tights) is available for easy online purchase. 🛍🛒🎁
I don't know about you but I am loving the little kicking girl on the back of the tights.
Flat rate shipping to US and UK. 📦
Shop now https://fitify.com.au/pink-belt-project/
What to know more about Pink Belt Project? Visit this dedicated blog page.


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Chinese payment platform Alipay will launch facial recognition payment services at 300 bakeries in Beijing, showing traditional retailers' resolve in adopting new technologies.
Popular bakery brand Wedome on Thursday said that all its customers would be able to pay their bills by scanning their faces when shopping at its Beijing stores. The so-called "Smile-to-Pay" solution, provided by Alipay, has so far been deployed at a number of branches in the city and will later be accessible in over 300 shops in the nation's capital.
Users are required to authenticate their identity via SMS when using the service for the first time. The recognition process takes up to 10 seconds, Alipay operator Ant Financial said in a statement.
However, a spokesperson from the company told TechNode that customers would be asked to verify their identity on their phones if the system detects "risky" surroundings—those that could pose a threat to a user's property and facial data.
"The system extracts the minimum amount of facial feature data necessary to verify the payment, and cannot be accessed by merchants," the spokesperson said.
This is not the first time Alibaba has teamed up retailers and restaurant to offer facial recognition payment services. Thai supermarket chain CP Lotus became its first partner utilizing the service in the Chinese market. It has also been introduced in all Alipay self-service point-of-sales terminals, including those in the Yum China's KPRO stores in Hangzhou.
Alipay's "Smile-to-Pay" solution debuted in 2015. The company announced a major upgrade earlier this month during its Open Day event in Shanghai, with the launch of a miniaturized version of the product.
Facial recognition technology is widely used in both for commercial and government purposes in China. In September, Tencent launched facial verification services to crack down on excessive gaming by minors. Last month, Shenzhen police "upgraded" their online WeChat services, allowing Chinese users to scan their faces rather than enter passwords to access public services.


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Very difficult!
I didn't realize you could throw the fish at first. If it was a longer game (also not a jam) I would advise ramping up a bit more gradually - for example the fish force field level doesn't really need the lasers to teach the player to throw the fish. It would still be puzzling at first without them, just less stressful.


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There comes a time when tough decisions such as creating a new website to replace the existing one have to be made. Luckily, Singapore is well known for having a plethora of professional web designers who have the skills and ability to make that happen for your business.
To help you make a decision on what to do with the current website that is not delivering the expected results, think of your site as a custom house. The total amount of money that you will need to fix and repair everything is mostly dependent on the quality of the materials, the age of the house, and complexity of the required update.
The same case applies to a website, if it can handle the current size of your business, it is worth refurbishing. However, it does not resonate with your business model; you should consider investing in a new website.
Here are two benefits of creating a new website instead of fixing the current one.
Saves Time and Money
One of the factors that web developers in Singapore consider when generating a quote for a customer is the amount of time that will be required to complete the site. From personal experience, trying to change another web developer's code is often tedious and time-consuming. This is the main reason why fixing a site is more expensive than creating a new one.
Access to Past Features
The statement "starting afresh" does not mean that everything on the current website should be thrown into the bin. Certain functions that are still relevant to your business can be retained or tweaked to make them more efficient and capable of scaling up your business. However, these incidents are very rare because technology has evolved tremendously over the years. It makes more economic sense to create a new website with new features instead of improving the old features that probably the target audience is accustomed to already.
The bottom line is; fixing an already broken website is usually expensive and does not yield significant positive results in most cases. If possible, consider hiring a Singapore web developer to create a whole new site for your business. If you wish to save costs – its possible to hire an reputable SEO service company to handle the error-fixing and re-optimise your site for higher rankings on the search engines.


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April 4th 2017
Come down, Frimousse!
I remember this picture too. Purrs to you on this day of sad memories.
Sringtime… Flowers growing over them… I'll show photographs when they're in full bloom, and when the sun shines.
These are great memories, Ah how we love them
Everytime I walk past the big organic shrimps in the supermarket… I nearly buy some for my Frimousse…
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9/11 is Charity Day
On September 11, 2001, 658 Cantor Fitzgerald and 61 Eurobrokers friends and colleagues perished in the terrorist attack on the World Trade Center.
Each and every September 11, we create a positive legacy for our loved ones by bringing together and redoubling our efforts. And this year, due to COVID-19, we are raising the money virtually.
On this special day, we invite our friends from the worlds of sports, entertainment, politics and business to join us on the trading floor. Cantor Fitzgerald and our affiliates, BGC Group and GFI Group, donate 100% of the day's global revenues to the Cantor Fitzgerald Disaster Relief Fund. The fund then distributes this money to over 150 charities. We lift lives by direct financial aid and assistance to hundreds of charities around the world.
To date, Charity Day has generated $192 million for people who need it – and we can't wait to raise more this year. You can join us by donating here.
Why it matters
From NBA stars to former Presidents, Oscar winners to supermodels, we have welcomed hundreds of celebrity volunteers into our offices to raise money and transform loss into hope. Learn more about why they believe in Charity Day.
View Charity Day images by year
"Charity Day is something which is unadulterated good. Everyone who comes in there, whatever walk of life they are, whether they are a politician or a sports star, you feel you're doing something worthwhile because you're raising money for good charities and there are thousands and thousands of people that are going to benefit from the work that you've done."
Tony Blair, Former UK Prime Minister
"Charity Day is about turning a tragic situation into a positive. I think that's what we do as Americans, that's what we do as New Yorkers when tragedy strikes, we find a way to be positive and what Cantor Fitzgerald does here today is such an important thing."
Jim Leyritz, Former MLB Player, NY Yankees
"To me, giving back means that you have been blessed, you have to do something to help those that need it. So on 9/11, people always remember a great tragedy and they redeem their losses by making it a day of hope for others."
President Bill Clinton, 42nd President of the United States


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Setting up to fail
Setting up to fail is a phrase denoting a no-win situation designed in such a way that the person in the situation cannot succeed at the task which they have been assigned. It is considered a form of workplace bullying.[1]
There are also situations in which an organization or project is set up to fail,[2][3] and where individuals set themselves up to fail.[4]
The first known documented use of "set up to fail" was in 1969 in the United States.[5]
In the workplace[edit]
Setting up to fail is a well-established workplace bullying tactic.[6][7][8] One technique is to overload with work, while denying the victim the authority to handle it and over-interfering;[9] another is the withholding of the information necessary to succeed.[10]
If a person puts another individual (usually a subordinate) in a stressful situation in which failure is almost certain, this may be an aspect of bullying wherein the outcome can then be used to discredit and blame the victim.[11] Sometimes, this may involve the bully covertly sabotaging and undermining an objective that may have otherwise been achievable. This type of bullying may be the result of the projection of the bully's own feelings of inadequacy onto the victim.[12]
There can be cases where an employee is set up to fail because the stated goals of the task are considered harmful to the organization; an internal investigation is one example. Institutions may protect themselves by "going through the motions" of a sham investigation in which the findings conveniently fail to find any evidence of wrongdoing by the authorities involved with setting up the investigation.
Another case where employees are set up to fail is one in which new employees, or redundant employees, are considered harmful or a threat to other employees, resulting in their efforts to sabotage others work to maintain their positions if a future reduction in force is anticipated.
Minorities seeking positions in society are often set up to fail in the face of covert institutional racism or sexism – something feared for example by the first Black US naval officers.[13] Concurrently, individuals from the larger group can be set up to fail during implementations of workplace programs in color consciousness.
Parents may have excessive expectations for their children's academic success for instance, thus setting them up for failure by hoping they may solve their parents' problems for them.[14] The result may be to create a self-destructive syndrome in the child – the so-called Divine Child complex.[15]
Therapy may be sabotaged by either the client or the provider. The client, both hoping for and fearing the possibility of real help, may impose conditions on the therapy that all but guarantee its failure.[16] Conversely, the helper, needing to keep clients in a state of dependency,[17] may be threatened by the prospect of success/closure, and undermine the therapy accordingly.[18]
Setting oneself up to fail[edit]
A person setting themselves up for failure may do so because they have a fear of failure, an unrealistic assessment of their own abilities, or because they are naive and uninformed regarding the abilities necessary to succeed. In some cases, an individual has an unjustified expectation that they will fail, a self-reinforcing negative spiral,[4] or failure neurosis[19] – perhaps driven by a sense of guilt,[20] or by the compulsion to repeat self-destructive behaviour.[21]
In television[edit]
It is a tactic used in reality television, where situations are engineered to produce certain results.[3] My Kitchen Rules contestant Emily Cheung told the reporter that "she believes the producers set them up for failure in the instant restaurant round when they were told at 6 o'clock the night before they had to cook a Chinese dish they weren't familiar with—smoked quail—and scored 2 out of 10".[3] The same article goes on to state that, "A former Apprentice contestant feels similarly manipulated, saying he believed producers had already decided who they wanted to win when he was eliminated."[3]
9/11 Commission[edit]
9/11 Commission member Lee H. Hamilton was quoted as saying that "the Commission was set up to fail"; some observers interpreted this as meaning that he was dissatisfied with the results of the 9/11 Commission Report, and conspiracy theory developed. The context of the interview transcript indicates that Hamilton said his reasoning was that "Tom Kean and I were substitutes—Henry Kissinger and George Mitchell were the first choices; we got started late; we had a very short time frame—indeed, we had to get it extended; we did not have enough money—3 million dollars to conduct an extensive investigation. We needed more, we got more, but it took us a while to get it."[2]
In popular culture[edit]
- In the 1967 film The Producers and its later adaptations, two Broadway producers try to set up a show to fail by intentionally selecting an offensive script.
- In the film The Hudsucker Proxy a corporation attempts to find a "dimwit, a proxy, a pawn, somebody we can really push around" for CEO, in order to manipulate the stock price to crash so that the board of directors can gain greater control of outstanding shares.
- Reginald Perrin tried to set himself up to fail by starting a shop called Grot, which only sold useless goods.
See also[edit]
- ^ Stambor, Zak."Bullying stems from fear, apathy." APA.com. Originally published in Monitor on Psychology. American Psychological Association. July/August 2006, vol. 37, no. 7. p. 72
- ^ a b Quotes - 9/11 Commission member said Commission was set up to fail.Skeptic Project. Accessed September 5, 2015.
- ^ a b c d Fenton, Andrew and Anna Brain. "Insiders say sleep deprivation and setting contestants up to fail are common tricks of the trade". The Daily Telegraph (Australia). September 5, 2015. Accessed September 5, 2015. Originally from news.com.au Archived 2020-06-20 at the Wayback Machine.
- ^ a b R. E. Boyatzis/A. McKee, Resonant Leadership (2005) p. 156
- ^ Economic Opportunity Amendments of 1969: Hearings, Ninety-first Congress, First Session, on H.R. 513
- ^ Peyton, PR. Dignity at Work: Eliminate Bullying and Create a Positive Working Environment (2003)
- ^ Rayner C, Hoel H A. "Summary Review of Literature Relating to Workplace Bullying", Journal of Community & Applied Social Psychology, volume 7, issue 3, pages 181–191, June 1997
- ^ Randle J Workplace Bullying in the NHS (2006)
- ^ J-F Manzoni/J-L Barsoux, The Set-up-to-fail Syndrome (2007) p. 5 and p. 12
- ^ Tim Field, Bully in Sight, Success Unlimited (1996) pp. 63–7 ISBN 978-0-9529121-0-1
- ^ Tim Field, Bully in Sight Success Unlimited (1996) p. 43 ISBN 978-0-9529121-0-1
- ^ S. White, An Introduction to the Psychodynamics of Workplace Bullying (2013) pp. 31–2
- ^ Paul Stillwell/Colin L Powell, The Golden Thirteen (2003) p. 98 and p. 86
- ^ Debra Wesselmann, The Whole Parent (2003) p. 104
- ^ Polly Young-Eisendrath, Women and Desire (London 2000) p. 107 and p. 113
- ^ Neville Symington, Narcissism (2003) p. 70
- ^ P. Casement, Further Learning from the Patient (1990) p. 144
- ^ Eric Berne, Games People Play (1966) pp. 126–7
- ^ De Mijolla, Alain. "Failure neurosis". Enotes. Retrieved 8 April 2013.
- ^ Otto Fenichel, The Psychoanalytic Theory of Neurosis (1946) p. 506
- ^ S, Freud, On Metapsychology (PFL 11) pp. 292–3


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The presence of microplastics in the environment and our food-chain is of growing concern. This has led to increased testing for the presence of microplastics in a variety of samples including bottled, ocean and fresh water, which has brought about tougher legislation to limit the amount of plastics entering the ecosystem. Fourier Transform Infrared (FTIR) and Raman spectroscopies have long been used for the analysis of polymers, and it is, therefore, natural that they be the de facto techniques to identify microplastics. This note provides an overview of FTIR and Raman techniques as applied to the identification of microplastics.
Fill out the form to download the Guide to the Identification of Microplastics by FTIR and Raman Spectroscopy.
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Extracellular vesicles (EVs) are a diverse group of particles secreted by all living cells. Numerous different therapeutic applications of both native and engineered EVs are currently in different stages of clinical development. Nevertheless, considerable challenges are still present in the manufacturing, purification and analytics of EVs. Many factors can influence the final product, therefore an all-inclusive approach to development of the bioprocess is needed. Cell culture parameters and production platform selection might alter the number and composition of EVs. Furthermore, raw materials used in upstream production, such as media and supplements, can greatly impact the chromatographic purification. In this study, we evaluated EV production in different HEK293-derived cell lines. Separation on a strong anion exchange column CIMmultus®-EV was used to assess the abundance of different EV populations. Multi-detector PATfix® SEC analytics coupled with antibody labeling was then used to analyze chromatographic fractions. Furthermore, the analytical methods and performance in downstream processing were applied in the optimization of the upstream process.
Cells release extracellular vesicles (EVs) of different sizes and intracellular origin. Due to their heterogenicity, the isolation of the target EV population from a mixture of supernatant-derived particles can be challenging. Anion exchange chromatography (AEX) exploits the negative charge on EV surface molecules for binding to the positively charged solid phase. CIMmultus® EV, an AEX chromatography monolith column, can separate EVs in subpopulations based on charge and offers insight into the heterogenicity of particles. Besides the availability of preparative tools for separation, combining multiple orthogonal and complementary characterization tools is crucial for defining the EV product of interest. In this work, we used a multiple-detector PATfix® system for the analysis of CIMmultus EV-fractionated samples. Samples were analyzed for the presence of EV-related tetraspanins using the fluorescence detector. PATfix MALS 3609 detector was used for the analysis of particle-containing samples and calculation of particle sizes.
mRNA has been at the forefront of both scientific and general public interests from the start of the COVID-19 pandemic. However, there are still limited options available for rapid characterization of mRNA containing samples. For precise characterization of an mRNA sample, first the presence and concentration of mRNA molecules in the sample needs to be identified. In the second step, any contaminants in the sample coming from the IVT reaction need to be identified and quantified. All major components of the IVT reaction; nucleotides, capping reagent, enzymes and DNA template may be present in the mRNA sample. In addition, impurities such as shorter, incomplete RNA fragments, and in particular, dsRNA may also be present. Contaminants may also come from the mRNA in vitro instability, caused by spontaneous hydrolyzation of the mRNA backbone. These issues can be mitigated using appropriate analytical tools throughout the mRNA production and purification steps.
Adenovirus is well-know gene therapy tool that gained attention as a promising vaccine delivery vehicle, specially during Covid-19 pandemics, where it was used to deliver sequence for protein S (S). Multiple serotypes have been tested in clinical trials for various applications, the most common one being human adenovirus serotype 5 (Ad5). With this in mind, we chose Ad5-S construct with GFP tag as a model vector to develop upstream process (USP) and supporting analytical tools.
Recombinant adeno-associated viral (rAAV) vectors are the leading gene delivery tool for treatment of a variety of diseases. While several rAAV mediated therapies have been approved so far, and many more are in clinical trials, rAAV production still faces many challenges. Key goal of rAAV upstream process development is achievieng high viral titer together with a sufficient percentage of full capsids. Moreover, analysis of complex upstream harvest samples can be challenging. Classical analytical methods such as ddPCR/ELISA offer limited information due to differences in sample preparation and basic principles for detecting empty and full capsids. Method is also time consuming and therefore less useful for following rAAV production process in real time. To overcome these limitations, we developed a PATfix Valve Switch analytical method that is based on ion exchange biochromatography and can be successfully applied for analysis of empty/full ratios in crude upstream samples.
Extracellular vesicles (EV) are lipid bound products secreted by cells. Among them, exosomes have great potential for clinical applications. Animal and human-derived components used in cell culture, such as fetal bovine serum (FBS), naturally contain exosomes that can cross-contaminate the desired product. In order to study exosomes derived from cells of interest, multiple producers have come up with exosome-depleted FBS (EV (-) FBS) generated using different approaches. In this work we evaluated commercially available EV (-) FBS supplements for residual exosome content and tested their performance in upstream exosome production process. The analysis was performed with PATfix high pressure liquid chromatography system using PATfix size exclusion (SEC) analytical method.
The IVT reaction is one of the most expensive steps in mRNA production process and its optimization to reach high mRNA yield is of key importance Standard mRNA quantification techniques like absorbance and fluorescence based assays are time consuming and cannot be performed at line as the IVT reaction progresses In addition, other reaction components like nucleotides and pDNA interfere in the analytical results and reduce the method's accuracy A new approach shown here uses CIMac PrimaS™ analytical HPLC column to separate and quantify several key IVT components with a very short run time, enabling fast "at line" tracking
Density gradient ultracentrifugation (DGUC) is a well established tool for Empty/ Full AAV capsid separation based on density differences between AVV sub-populations. However DGUC practice is laborious and lacks any detection options, therefore fractions must be collected manually and analyzed later. Both of these shortcomings can be addressed by coupling post DGUC workflow to PATfix analytical system. BIA Separations PATfix platform provides sufficient tools for liquid extraction and fractionation as well as a comprehensive detector suite for precise fraction characterization. Baseline separation of capsid species was achieved in a density gradient of CsCl, producing a centrifugram that reveals information traditional DGUC and anion exchange chromatography cannot.
Optimizing processing steps in sc pDNA isolation is critical for obtaining good process yields as well as high product purity. PATfix platform with convective chromatography media (e.g. monolith) offers a rapid analytical method to characterize complex biomolecular mixtures and gives immediate feedback during process development. E coli lysis represents such a challenging step, where multiple critical quality attributes need to be identified and critical processing parameters optimized. This approach leads to better yields and product purity, allowing for simplified downstream steps. A new PATfix analytical platform presented here uses CIMac pDNA column, to separate and characterize plasmid from impurities, allowing for easy optimization of key parameters such as RNA removal.
Removal of host cell DNA is essential for all human-injectable biologics. This poster shows a method for achieving low host cell levels in preparations of exosomes. Purified exosome samples were prepared with anion exchange chromatography (AEC) and pre-treated with tangetial flow filtration (TFF) and nuclease treatment. Results are compared with an experimental control using TFF and size exclusion chromatohraphy (SEC).
The steps in purification process are illustrated by analytical size exclusion chromatography (SEC) on PATfix system with in-line UV, MALS and fluorescence detectors and by staining with Picogreen reagent. This technique visualizes sample composition by size, UV, light scattering and fluorescent properties.
AAV vector lots are generally a heterogeneous mixture of empty particles (i e do not contain DNA) and full particles (i.e. contain DNA). Different spectrometric based methods can be used to establish the ratio between full and empty AAV particles, but accurate evaluation of empty/full ratio is often obstructed due to complex spectroscopic behavior of empty and full AAV particles, such as poor separation and impurity overlapping. An approach that takes difference in physical chemical properties between empty and full capsids into account overcomes limitations of spectrometric based evaluation of empty and full AAV particle ratio.
Chromatographic separation of empty and full AAV 2 8 capsids was achieved on the CIMac AAV full/empty analytical column (strong anion exchanger, QA quaternary amine chemistry) with the PATfix™ system using a linear NaCl gradient at pH 9.0 Signal response from three different detectors connected in series was analyzed fluorescence (excitation 280 nm emission 348 nm), light scattering 90 angle, LS) and UV absorbance 260 nm and 280 nm).
Exosomes fulfill a critical role as communicators among cells, with targeting and message content depending on their surface receptors and payload. This makes them obvious candidates for an extensive range of diagnostic, therapeutic applications and a need for a fast, robust and scalable purification procedure.
CIMmultus™ monolithic columns are designed to meet the special fractionation needs of very large biologics like exosomes.
We show examples of exosome purification from cell culture with CORNERSTONE Exosome Process Development Pack and analysis of exosomal vesicle populations by Image stream flow cytometry.
This poster shows how Multi-Angle Light Scattering detector and Fluorescence detector couppled to PATfix analytical system can be used to track extracellular vesicles through purification process. Samples were analyzed by analytical size exclusion chromatography (SEC). On SEC cell culture components diffuze into pores of chromatographic media and are separated (mostly) based on size. Particles larger than the media pore size are excluded in the void peak. This peak represents extracellular vesicles including apoptosomes, microvesicles and exosomes as well as cell debris and aggregates.
Production of high value biological therapeutics usually involves complex manufacturing processes with high process variability. Additionally, development of robust and reliable bioprocesses can be challenging. PAT aims to enhance bioprocess understanding and implies a holistic approach to ensure that quality is built into products by design. Efficient PAT therefore calls for fast and robust analytical techniques which enables to asses high quality information about critical quality attributes and key performance indicators as parallel as possible to the manufacturing process. PATfix™ is unique analytical system for routine gradient separations that enables every analytical task. Equipped with bio-inert ceramic pump heads is deliberately tailored to meet the demands of analytical applications covering wide range of biomolecules. Highly sensitive and fast multi-wavelength detector enables to detect component peaks even in very fast gradients.
Productivity of the downstream bioprocessing depends among others on the efficiency of chromatographic step. One of the crucial chromatographic parameters is dynamic binding capacity (DBC) for certain biomolecule. DBC could be tailored with changing the surface area of convective pores by tailoring the surface of pre-polymerized monoliths using graft or block polymerization of polymer brushes. Grafted CIM monoliths have already been prepared via Radical Polymerization (RP) and successfully characterized (1).
Recently, the implementation and optimization of Controlled Radical Polymerization (CRP) for grafting of large pore monoliths (average diameter 6 μm ) resulted in polymethacrylate-based ionic exchanger with at least 5 times higher DBC compared to non-grafted 6 μm monoliths, while preserving high permeability. The main goal of our study was to chromatographically characterize novel grafted ion-exchanging monoliths (CIM gDEAE and CIM gSO3) to see whether novel columns still retain flow independent chromatographic properties of non-grafted monoliths.
To ensure the desired chromatographic characteristics of the CIM® monolithic column at large scales, monolith microstructure morphology, pore size distribution, porosity and surface ligand density should be uniform. To demonstrate the uniformity of large chromatographic monoliths we have developed new testing procedures. By fabricating smaller columns (disks) from different random positions of larger monolith, non-cGMP compliant chromatographic testing can be applied on the same polymerization batch without affecting the cGMP compliance of large-scale chromatographic monolith. Each individual disk was thoroughly tested and the results were compared to the properties of the large monolith.
There are many cases, where a single protein needs to be purified from a complex sample. Such proteins manifest themselves as impurities, which can affect further analysis, either by causing specific equipment malfunction or lower yield in the products. In other cases the specific protein is our molecule of interest, for example in glycomics analysis. In both cases high specificity for proteins, reproducibility and reliability is necessary. We have developed a model immunoaffinity column and 96-well plate based on an anti-fibrinogen monoclonal antibody, covalently immobilized onto CIMac™ analytical chromatographic monolith.
There are many cases, where a single protein needs to be purified from a complex sample. Such proteins manifest themselves as impurities, which can affect further analysis, either by causing specific equipment malfunction or lower yield in the products. In other cases the specific protein is our molecule of interest, for example in glycomics analysis. In both cases high specificity for proteins, reproducibility and reliability is necessary. We have developed a model immunoaffinity column and 96-well plate based on an anti-fibrinogen monoclonal antibody, covalently immobilized onto CIMac™ HDZ analytical chromatographic monolith.
Methacrylate monoliths (CIM® monolithic columns) allow for very fast and efficient separations and exhibit very high binding capacities for extremely large bio-particles due to their large inner channel diameters and enhanced mass transfer characteristics.
Additionally, the ability to manufacture polymer monolithic materials ranging from analytical to large scale preparative/industrial columns has tremendous advantages. By ensuring the chromatographic properties are consistent over the whole size range, one can easily design and optimize a purification method on laboratory scale and transfer it to a production line with minimal to no additional modifications.
Until now the largest monolithic column had a volume of 8 L, which was large enough to serve the biopharmaceutics' market's needs. Now however, the capacity of that column is already at its upper limit.
By successfully employing the knowledge and experience from almost two decades of monolith production we have managed to overcome the size limitations and polymerize the largest convective chromatographic support made from one piece of material, a 40 L monolithic column.
Immunoaffinity columns using antibodies as ligands against mammalian membrane proteins could be used for different applications in protein expresion control and, if a standard available, for concentration determination. Additionally these columns are ideal for polishing step of Fc fusion proteins of mammalian receptors.
Most importantly such columns could extract a significant amount of a pure membrane mammalian protein suitable for structural analyses, such as mass spec analysis of their glycans. Immunoaffinity chromatographic monoliths against MULT-1 transmembrane and RAE-1 GPI anchored glycoproteins were developed as a part of Glycomet project with the main goal to analyze the antigen glycan parts.
Two different preactivated support were used: hydrazide (HDZ) and carboxy imidazole (CDI).


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Have you ever heard it said that a woman should be a help meet (or helpmate) to her husband? How did that make you feel? Today, my guest Elle E. Kay shares her perspective on what, exactly, being your spouse's help meet actually means. But first, a caveat–Elle is not saying a wife must be a doormat, or that she should completely lose who God uniquely created her to be. Instead, she is expressing how she adapts her behaviors so that they have the greatest impact in conveying love and support. Hopefully, her husband is doing the same, but she has no control over that. All she can do is love her husband and love him well.
Being His Help Meet
by Elle E. Kay
Some may think that there is something degrading about being a man's help meet. If you explore it from a biblical perspective, you realize that it is an honor. God made man. He then set out to get man a help meet (Gen 2:18-20). In the process of choosing a help meet, Adam was shown that there were no creatures suitable for his needs. God made Eve from Adam's own rib bone (Gen 2:21). She was a precious gift. A woman who was "meet" (suitable, proper, fitting) to satisfy his needs.
When I think about it, I realize that in agreeing to wed my spouse, I agreed to be the woman who would meet his needs. To be a suitable mate in every way. If I set out to do that in our daily lives, we are both happy.
Every man is different and has different needs. My husband is a strong independent male. He wouldn't be happy with me fussing over him all the time. There are some men who want just that and there are some women who are happy to provide that. My husband wants a partner who will handle the things he doesn't like to do and who will depend on him to do "manly" things. He's a carpenter. He likes to build things. It made him happy to make me a pool shed, a barn, and a chicken coop. He's also a gentleman and enjoys opening doors for me. That doesn't mean that he doesn't need or want my help. He does. He may never vocalize his needs, but if I pay attention, I'll know them. He doesn't like to put away laundry. I do that. He enjoys a good meal, I enjoy cooking for him.
The point is, I fill in where he needs me. I don't try to fit some ideal of a perfect wife. I simply do what makes him happy. My house is rarely perfectly clean and dust free, but the things that need to be done are done. The things that drive him crazy like a sink full of dirty dishes are avoided (most of the time). In turn, I get the satisfied feeling of knowing I've met his needs. We've all heard the expression "happy wife, happy life." It works just as well in reverse. If we spouses rise up to the challenge and do the things that make our husband's lives easier, they will be happier. If they are happy, we are happy.
I didn't say anything about a proper help meet staying home cooking and cleaning. A proper help meet can only be defined by the needs of her spouse. Barring that I would look to Proverbs 31. A Proverbs 31 woman does a lot more than dust and vacuum her home.
I would be remiss if I didn't mention one other small thing. The things I do for my spouse, I must do without complaint. It wouldn't make anyone happy if I walked around the house mumbling and grumbling as I went about my business.
"Do all things without murmurings and disputings" (Philippians 2:14, KJV).
We are designed to help our husbands, but that doesn't mean we must agree on every matter. How much help would we be if we simply nod our ascent as our husbands drive our families off the edge of a cliff? Sometimes we need to speak up. Help comes in many forms and may not always be easy. God designed us to be up for the challenge.
Abandoned by her dearest friend, Stella is running-scared. Life and death decisions force her to re-examine her faith, as well as her priorities. The handsome, Jason, only exacerbates her anxiety. Should she trust him? Something is amiss in the quiet town of Edinsville. How will Stella fare as her world gets turned upside down?
Elle E. Kay lives on a farmette in the Back Mountain region of Pennsylvania. An introvert, she surrounds herself with farm animals rather than people most of the time. But once you break down her initial walls, she can be quite talkative.
Connect with Elle on Facebook, Amazon, Twitter, and her website.
Let's talk about this: So often, society puts a negative connotation on something God creates to be beautiful. Have you experienced this? How do you strive to be a help meet to your spouse? How has that blessed you and your marriage? Share your thoughts in the comments below or over on Living by Grace. I'd love to hear them!


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primary colors table lamp
Metal table lamp with primary color palette. Stunning on a desk.
sourced in: Belgium
dimensions: 32 cm x 13 cm x 13 cm
weight: 734 gr
This is a vintage item. It may show wear consistent with age and use, and are in good vintage condition without major damage unless otherwise stated.


text: Simplify the Clinical operations in the most effective way with the Clinic Chain Management Software. Doctors don't have to hunt through the medical health records any more as this software shows you the digitized format of the records. It even helps you track the inventory and distribute them accordingly. You can even send the reports via email and SMSs.


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Application Note
3D analysis and morphometric characterization of compound effects on cancer spheroid cultures
- Perform high-throughput screening of spheroids for efficient and consistent acquisition
- Quantify and measure characteristics of more physiologically-relevant models
- Reduce analysis time using an integrated software solution
Cellular transformation/tumorigenicity assays using cultures of cells in semi-solid media (soft agar or Matrigel) has been well established for cancer research1,2,5. The assay requires cells to grow in an anchorage independent manner, which is a hallmark of cancer cells. Compared with adherent cells grown in 2D monolayers, 3D growth conditions are believed to more accurately reflect the natural environment of cancer cells and span the gap between 2D cultures and animal studies3,5. Importantly, 3D assays correlated better with tumorigenicity in animals, e.g. mouse xenografts5. The growing interest in developing methods for personalized medicine, in which tumor cells from individual patients are tested for sensitivity to a panel of drugs4, has led to a need for more relevant, time-sensitive studies.
3D assays were formerly labor-intensive and inconsistent due to the subjective definitions of colonies and, non-suitable for high-throughput screening. In this study, we describe a method compatible with highthroughput screening, in which compound effects on cell growth in semi-solid media were measured and quantitated using high-content imaging and 3D analysis.
Experimental protocol amenable for screening
To prepare spheroid cultures in semi-solid media, human colon carcinoma HCT116 cells were pre-mixed with a solution of 4.25 mg/mL cold Matrigel (Corning) diluted in growth media and plated into 96-well half-area plates (Greiner) at a density of \~500 cells/well for a total volume of 25 μL. The plate was incubated for 30 minutes at 37°C to solidify the gel, followed by the addition of 25 μL of media. Within 24-48 hours, spheroid colonies formed in each well.
The resulting spheroid cultures were treated with anti-cancer compounds for 5 days. Compounds were diluted in media at 2X concentrations and 25 μL of a 6 point dilution series was added to triplicate wells. On day 3, we added fresh compound by exchanging half the media with 1X compound dilutions. At the conclusion of treatment with test compounds, cells were stained with a mixture of two dyes: final concentration 1 μM calcein AM and 5 μM Hoechst 33342 (Life Technologies). Dye solutions were prepared at 3X concentration and added directly to media without aspiration and incubated for 2 hours prior to imaging. In an alternative assay, cells were fixed for 1 hour using a 4% formaldehyde solution, washed 3x with PBS by removing half of the volume each time. Then the cells were stained with 5 μM Hoechst 33342 and 0.06 μM AlexaFluor-488 Phalloidin (Life Technologies).
Integrated confocal imaging and 3D analysis
Images were acquired using ImageXpress® Micro Confocal High-Content Imaging System, with a 20× Plan Fluor or 10× Plan Fluor objectives (Figure 1). A z-stack range of 100-150 μm was acquired with a series of 11-20 z-slices separated by 5-10 μm (for 10x magnification) or 2-5 μm (for 20x magnification). Two sites were imaged per well and all individual z-slices, as well as the 2D maximum projection image for each z-stack, were saved for 3D analysis (Figure 2).
Figure 1. Maximum projection image of spheroids in Matrigel stained with Hoechst and AF488-phalloidin.
Figure 2. Z-planes of spheroids in Matrigel. The spheroids were stained with Hoechst and AF488- Phalloidin. Distance from the bottom of the well is indicated.
Images were analyzed using the 3D analysis module within the Custom Module Editor of MetaXpress® High- Content Image Acquisition and Analysis Software. Spheroids and individual cells were defined based on size and intensity. Then, spheroid size and phenotype were characterized by volume, diameter, and fluorescence intensities of cellular markers. Multi-parametric outputs were generated using a customized analysis module (Figure 3) and the readouts for each individual spheroid included measurements for nuclear count, size, volume, intensity, live cells per spheroid, whole cell volume and diameter, spheroid diameter, and average intensities of cellular markers (calcein AM, Phalloidin, or Hoechst). The readouts described above can be reported per individual object (spheroid) or averaged per well. Additional sites per well can be acquired to increase the number of cells scored. IC50 values were determined using a 4-parameter curve fit (Figure 4).
***Figure 3. Definition of spheroids using MetaXpress Custom Module Editor. (A)*The Count Nuclei and (B) Cell Scoring modules defined the spheroid count and individual cells were scored for each marker of interest.
Figure 4. Concentration-responses for selected compounds. Measurements of (A) number of spheroids/well, (B) number of live cells/well, and (C) the sum volume of live cells/spheroid were calculated within the 3D volume.
Multiple 3D parameters show morphological effects of compound treatments
We observed significant changes after compound treatment. In general, there was a reduction in the number of spheroids, as well as the number of cells per spheroid consistent with expected anti-proliferative effects. We also observed a concentrationdependent decrease in the sum volume of live cells within each spheroid, the spheroid size, and the number of viable cells. The intensities for calcein AM or Phalloidin staining were dramatically reduced for the spheroid and also for individual cells. Measurements of the number of spheroids in the 3D volume were used to calculate the IC50 values showing the response to the anti-cancer compounds (Table 1).
, μM
Table 1. IC50 values measured for selected compounds using number of spheroid per well as a readout.
3D spheroid cell models combined with high-content 3D assays show promise as a screening tool for assessing compound effects, proliferation, and viability of cancer cells. Assays can be used for drug development, drug re-purposing, and personalized medicine.
- Grenman, R., Burk, D., Virolainen, E., Buick, R.N.; Church, J.; Schwartz, D.R.; and Carey, T.E. (1989). Clonogenic cell assay for anchorage-dependent squamous carcinoma cell lines using limiting dilution. International Journal of Cancer, 44(1), 131–136.
- MacPherson, I., & Montagnier, L. (1964). Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology, 23, 291–294. Retrieved from Pubmed.
- Pampaloni, F., Reynaud, E. G., & Stelzer, E. H. (2007). The third dimension bridges the gap between cell culture and live tissue. Nature Reviews Molecular Cell Biology, 8(10), 839-845. Retrieved from Pubmed.
- Rotem, A., Janzer, A., Izar, B., Ji, Z., Doench, J. G., Garraway, L. A., & Struhl, K. (2015). Alternative to the soft-agar assay that permits high-throughput drug and genetic screens for cellular transformation. Proceedings of the National Academy of Sciences, 112(18), 5708-5713.
- Shin, SI., Freedman, VH., Risser, R., & Pollack, R. (1975). Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro. Proceedings of the National Academy of Sciences, 72(11), 4435–4439. Retrieved from Pubmed.
Learn more about the ImageXpress Micro Confocal High-Content Imaging System >>


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"…we have taken the time that was necessary for development and testing to be able to offer a perfect product in the end. We aren't entering a new segment to finish second."Stefan Wendl, head of Mercedes-AMG Customer Racing
With the new race car, Mercedes-AMG GT4 is entering another GT racing category. Race series all over the world provide an attractive mix of demanding sprint races and legendary endurance races that allow for an increasing number of opportunities to compete with GT4 machinery.
As with the introduction of the SLS AMG GT3 and the Mercedes-AMG GT3, aspirations for the new race car are high. The Mercedes-AMG GT4 is entering the scene as a fully matured and proven competition car. It arrives to set new benchmarks in its class relating to safety, ergonomics, driveability, performance as well as service- and user-friendliness.
As Test and Development Drivers, Bernd Schneider, Thomas Jäger and Jan Seyffarth are largely contributing to the maturity of the Mercedes-AMG GT4. Like with the SLS AMG GT3 and the Mercedes-AMG GT3, the co-operation between the experienced drivers and the engineers from Affalterbach is an important element in the complex vehicle development process. The close interaction between the experts results into excellent driveability, a characteristic that is typical for the Mercedes-AMG Customer Racing cars.
The Mercedes-AMG GT4 is powered by the 4.0 litre V8 biturbo engine with direct fuel injection, known from the road cars from Affalterbach. The proven production technology of the front-mid engine allows for particularly long service intervals and also provides sufficient performance reserves for the future. Depending on the balance of performance, this results into an output of up to 510 hp and a maximum torque of over 440 lb-ft.
To achieve maximum performance at the race track, the Mercedes-AMG GT4 has been weight-optimised in every area. An intelligent mix of aluminium and carbon has been used for the chassis and the bodywork. The structure of the GT4 consists of a very light and extremely rigid aluminium spaceframe that can also be found in the Mercedes-AMG GT R and that has been honoured with the 'Leichtbau Award 2017', an innovation award from the German engineers' association VDI. As a part of the AMG Lightweight Performance Strategy, further measures and optimisations of details contribute to the weight reduction. Thus, racing-specific bodywork parts such as the bonnet, the front apron or the front splitter have been made of a particularly lightweight composite material. In the production version of the GT R, carbon is already used as a material, for instance for the front wings and the torque tube between the engine and the gearbox.
The main areas of competition for the Mercdes-AMG GT4 The boom of the GT4 class was initiated in Europe and is reflected in a rapidly increasing number of opportunities for competition, be it with exclusive GT4 formats or as separate vehicle categories within various series or events. Currently, the following series can be considered as the most important 'hunting grounds' for the new Mercedes-AMG GT4: the IMSA Continental Tire Sportscar Challenge and the Pirelli World Challenge in the US, the Blancpain GT Series Asia in Asia and the Australian GT Trophy in Australia. In Germany, the VLN endurance racing championship and the Nürburgring 24-hours are the designated highlights for the new race car from Affalterbach. The GT4 European Series Northern Cup and the GT4 European Series Southern Cup are dedicated international GT4 series. The 24H Endurance Series also provides a large international platform for GT4 competition. Therefore, the new Mercedes-AMG GT4 is eligible to race at a large international platform where it can compete for victories and titles in the hands of the AMG Customer Racing Teams.


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Mobile app Shazam announced its latest milestone yesterday: 10bn tags. Lady Gaga's 'Applause' got the nod as the 10 billionth tag on the service, which launched in 2002 as a way for people to identify songs playing in the real world using their mobile phones.
Shazam also said yesterday that it's now processing 100m tags a week, up 150% on this time last year, while reminding us that its 70m active users currently buy more than $300m of digital music a year through the app – around 10% of all downloads.
"It took ten years for the company to reach its first billion, ten months to reach its second billion and now, the company has seen user activity accelerate so rapidly that it only took two months to go from nine to ten billion Shazams," said CEO Rich Riley in a statement.
But yesterday's figures also gave an insight into the relative importance of music and TV/advertising to Shazam, as part of this rapid growth. Earlier this summer, Shazam's executive chairman Andrew Fisher said that 54% of Shazam's users currently tag something on TV once a week. That's just under 38m people, indicating that at least 38% of those 100m weekly tags are TV-related.
And that tallies with another stat in yesterday's press release: that 100 songs were tagged in the same second as 'Applause' as the 10bn milestone was reached. Do the maths, and 101 songs a second scales up to just over 61m song tags a week.
A 60/40 split between music and TV tags would show how rapidly Shazam's TV and advertising business has grown, even though the company continues to invest in its music business: the music-tagging features have been the easiest way to get people using Shazam in the first place, so they're primed to pull out their phones when they see a Shazam logo on their TV screen, after all.
But thinking about iTunes affiliate fees versus payments from broadcasters and brands, it wouldn't be surprising at all to see Shazam's TV revenues overtake its music revenues sooner rather than later – if they haven't already.
Tools: platforms to help you reach new audiences
Tools: Kaiber
In the year or so since its launch, AI startup Kaiber has been making waves,…


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I updated my list of Bollywood movies on Netflix with more than a dozen new additions to the catalog. Ten of those films are Malayalam flicks from the last year or so, as well as the 2015 Hindi movie P Se PM Tak and the cartoon Mahabharat. I'm especially excited that Tikli and Laxmi Bomb is finally available for streaming. It had been on the festival circuit for the last year, and it's really thought-provoking. I recommend it.
I also updated my list of Bollywood movies on Amazon Prime with ten new Indian films. The 2018 Tamil theatrical release Thaanaa Serndha Koottam is now available for streaming, along with six Telugu movies, and the Bollywood flicks Amit Sahni Ki List, Main Aisa Hi Hoon, and 8×10 Tasveer (which is not very good).


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Bringing Out the Dead
Paramount Home Video
Starring Nicolas Cage, Patricia Arquette, John Goodman,
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Review by D. K. Holm
Context is everything. If Bringing Out the Dead had been the debut film of, say, Quentin Tarantino, it would have been heralded by reviewers as the start of a brilliant career. They would have exclaimed over its masterly command of the camera, its solid story structure, and its wise, scuffed-shoe knowledge of the street. But instead it was the 34th film, including short subjects and documentaries, by Martin Scorsese, and his fourth collaboration with screenwriter Paul Schrader (though doesn't it seems as if they've worked on many more films than that?). As such, the film was deemed a comedown, another deflection from the true trajectory of Scorsese's career toward that ultimate iconic gangster film to end all gangster films, which may indeed come to us some day in the form of a biopic about Dean Martin.
We demand more from a Scorsese film. We look to his work for solace in a field of otherwise mediocre, unimaginative, and visually impoverished mere "product" from Hollywood. In fact, we may demand too much, so much that we sometimes can't see the value of what is before our eyes, so that disappointment often evolves over the passing years into a certain regret that we didn't appreciate the genius of a given film at the time it was released (call it the Stanley Kubrick Syndrome). But if Dead failed to tickle the critics, it really failed to draw audiences. Bringing Out the Dead, released by Touchstone on the 24th of October, 1999, cost $32 million dollars, and in its initial run grossed a disastrous $16 million dollars, according to the IMDB. (By the way, if you think that the reception of Bringing Out the Dead is a bummer, consider the fate of Broken Vessels, Scott Ziehl's black comedy about drug addicted Los Angeles EMS service drivers starring Todd Field. Though it was made before Dead, it came out afterwards, and died in its toxic wake).
Seeing the film again on DVD confirms my initial impression that Dead is alive with emotion, action, comedy, and passion, and that it's one of Scorsese's best films focused, felt, funny in years. And he derives such an outstanding performance from Nicolas Cage that it's possible to forsee a long term collaboration between the two of them to rival Scorsese's association with Robert De Niro. This usually over the top actor was for once (for me), a joy to watch. One of the funniest moment occurs in Chapter 15 on the disc, when Marcus (Ving Rhames) asks Cage what the ghosts Cage sees tell him, and Cage, as he turns in his seat to face his partner, replies slowly with mounting comic mock craziness, "They say KILL MARCUS, OK?" And the way he assures John Goodman that he is not off his rocker enough to starve himself ("I eat. Larry. I eat") is beautifully delivered and filled with raw yet modulated emotion. Also, Cage proves to be an excellent physical actor. There is a simple beauty in the movements with which he kneels down to "bring out the dead" from their ghostly haunts below the street during one dream sequence.
The movie is based on an autobiographical novel by Joe Connelly. It comprises a delineated few days in the life of a EMS medic named Frank Pierce (Cage), who spent his early childhood in the very neighborhood of New York City where he now cruises for bruises and much worse. In keeping with a general "threefold" approach of the film, or "trinity" given that the pious Schrader is the writer, Pierce rides in succession with three partners, each one adopting a different means of dealing with the horror and insanity they face each night. First there's Larry (John Goodman), whose stomach dictates his behavior; then there's Marcus (Ving Rhames), whose blend of religious fervor and sexual seductiveness keeps his mind occupied and off the street; and finally there is Tom (Tom Sizemore), who takes the tack of acting as crazy as the people he helps. Meanwhile, Pierce has fallen for Mary Burke (Patricia Arquette), the daughter of a heart attack victim whose life he has prolonged. He follows her around the city when he is off duty, learning about her troubled past and helping her out of scrapes. Throughout all this, the haunted, increasingly cadaverous Cage sees the ghost of Rose (Cynthia Roman), a pregnant girl he failed to save one night. Pierce is seeking redemption or peace, and though there isn't a lot of suspense as to whether he is going to find it, the trip is powerful. This is one of the richest stories that Scorsese has brought to the screen in years, abundant in incident and subtle in nuance, and the cast is, at least to one viewer, brilliant at bringing out the passion.
If the film has a conceptual flaw it is the source of Cage's depression. Just what is its cause? He says it's because he's lost it, hasn't saved a life in months, or at least that is how his situation and mental state are accounted for narratively. But he is so depressed, can his lowspirits be caused solely by a a few incidents, especially when everyone else around him seems to be coping? Is he a burnt out case, or is there some larger moral or philosophical basis for his state of mind. Both Scorsese and Schrader embrace religious images and themes, and the fact that his sorrow is a prelude to Pierce becoming a God-like taker and giver of life is perhaps not as clearly delineated as it could be.
The 2.35:1 widescreen transfer to DVD is clean and sharp (though on the DVD I watched there were two moments of scrambling, at the end of Chapter 17 when Cage climbs out of a crashed ambulance, and a little later in Chapter 18 ). Robert Richardson's cinematography brings a cutting harshness to the city where the emotional Cage, a "grief mop," lives with his nerves frayed and searching for quietude and salvation. Richardson's custom of planting direct overhead lights that temporarily overexpose the actors as they move along their paths, as he has done in the many Oliver Stone films he has shot, both rhymes with the street-lamp illuminated world where the characters dwell, and also serves to brutalize them just a little more. His imagery survives well the transfer to DVD. A CGI moment in Chapter 28, when Cage sees muliple duplications of Rose in a group of women loitering on the street from his ambulance, is one of the rare instances where trick computer imagery actually contributes to the theme and tone of the film it is in.
Additional features of this DVD are limited, but not unhelpful. Scene selection is useful for film students. And a short interview documentary shows Scorsese and Connelly articulate and comfortable about the film, while Cage gropes as he tries to come to grips with what he is bringing to the film and what it demands from him. Two trailers are almost identical yet the second one somehow manages to convey more excitement about the film.
D. K. Holm
- Color
- Anamorphic widescreen (2.35:1)
- Single-sided, single-layered disc (SS-SL)
- Dolby Digital 5.1 (English)
- Closed-captioned
- Cast interview documentary
- Trailers
- Keep-case
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More Than Just Print On Paper
We are an environmentally friendly printers in Sussex and we are very proud of the quality of our traditional sustainable printing. We also offer a wide range of other print solutions including point of sale printing, roller banners, printed magnets, stickers and outdoor banners. We can even print custom wallpaper.
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A selection of pieces showing litho and digital Print, as well as some of our great finishing and binding techniques.
How To Use Our Online Print On Demand Service
Either simply upload your own print ready artwork, change simple variable data, or design your product from scratch.
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Here's the latest article from the African Culture site at BellaOnline.com.
Madagascar Hissing Cockroach Facts
Madagascar Hissing Cockroaches are only found in Madagascar. These roaches are kept as pets, adorned with jewels, and are simply very interesting bugs.
Please visit http://AfricanCulture.BellaOnline.com for even more great content about African Culture.
This Week's African Proverb - The laughter of a child lights up the house. ~Swahili proverb
To find this and other fascinating African Proverbs along with up to the minute updates on the African Culture site, go to http://Twitter.com/AfricanCulture
To participate in free, fun online discussions, this site has a community forum all about African Culture located here -
I hope to hear from you sometime soon, either in the forum or in response to this email message. I thrive on your feedback!
Have fun passing this message along to family and friends, because we all love free knowledge!
Jeanne Egbosiuba Ukwendu, African Culture Editor
Follow African Culture on Twitter at http://Twitter.com/AfricanCulture or become a fan on Facebook at http://tinyurl.com/AfricanCultureFB
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Feel inspired by the modern and contemporary design of our Sky Bar, Bar Mezanino and Restaurant. At Sky Bar you can enjoy our exotic cocktails with a unique view of Maputo's Bay. Continue your gastronomic experience in our restaurant Mercado da Baixa where you can taste a special selection of local and international dishes. Don't forget to explore our Bar Mezanino area where you can relax or even having an informal meeting.


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Monday, September 12th, 2016 7:10PM EST
Television: ESPN
Radio: Sirius
Online: Game Pass
Tickets: Ticketmaster (3,548 available of as this entry)
Kickoff Temperature: 75 degrees, 5 mph winds
Line: Pittsburgh -3
Over / Under: 50
Injury Report
DE Kendall Reyes (groin)—The story here is not so much about Reyes but what the Redskins will do on the defensive line. There are only six defensive linemen on the 53-man roster. Twice this week Jay Gruden said that there was no concern if Reyes is out because they might go with only five active defensive linemen even if Reyes is able to go. This would be a departure from previous seasons when the Redskins would normally go with six active on the D-line. This would create another spot for perhaps a defensive back or a special teams player.
DE Kedric Golston(back)—This injury just popped up today. Golston was held out of practice due to the injury. We did not get any details from Jay Gruden as he did not talk to the media after practice. In the locker room, however, Golston said that he would have been "probable" under the old injury designation. He also said, "I'm playing".
WR Josh Doctson (Achilles)—He was on the PUP list from the start of training camp up until a week ago and he has just five practices under his belt and he was limited in those. It would not be surprising if it is another week or two until he makes his NFL debut.
RB Matt Jones (shoulder)—He declared himself to be 95 percent yesterday and vowed he would be a full go on Monday. It will be interesting to see how he holds up if he lands on that left shoulder again.
G Spencer Long (ankle)—It's possible that the injury cost him his last shot at holding on to the starting job at left guard. The best guess is that he will be active and the first player off the bench if they need a guard or a center.
All of these players except Golston were full participants in practice on Saturday. Golston did not practice. OT Ty Nsekhe, who is listed as
The Steelers are starting out somewhat banged, with OLB Bud Dupree on injured reserve and TE Lararius Green on the PUP list.
WR Markus Wheaton (shoulder)—The fourth-year receiver would be the choice start opposite Antonio Brown if he was healthy but the shoulder has been a concern throughout the preseason. This leaves Sammie Coates s the likely starter.
FB Roosevelt Nix (back)—He is the only fullback on the roster but the Steelers will work around his absence.
CB Senquez Golson (foot)—The fifth of five cornerbacks on the depth chart, Golson perhaps would have been inactive even if he's healthy enough to play.
C/G Cody Wallace (knee)—Wallace is listed as the top backup at all three interior line positions so the Steelers will have to hope that the starters can make it through the game.
On the practice participation report this week but not listed on the injury report: Starters DE Cameron Heyward (ankle), DT Javon Hargrave (Quad), and reserves RT Marcus Gilbert (elbow), and DT Daniel McCullers (knee).


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Evoked potential EEG research makes even firmer the connection between severity of autism (ASD) and problems in auditory processing.
Tag Archives: asd
Differentiating Autism from Sensory Processing Disorders
Diffusion Tensor Imaging shows how Sensory Processing Disorders (SPD) are separate from Autistic Spectrum Disorders and Williams Syndrome.
Neurofeedback WORKS–How?
Neurofeedback efficacy is established. Mechanism of Action is complex and not well understood.


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by Erin Richardson
It is absolutely real! I am so thankful that I was able to recognize my son's SPD at such a young age. He has been receiving OT since he was 3years old and his symptoms are improving. I am literally in tears as I am reading the things on this website and the experiences of others. I wondered why my son always fell out of his chair, dropped his cup, and ran into the wall! I am excited to try some of the suggestions on this website to help my son improve.
Join in and write your own page! It's easy to do. How? Simply click here to return to Is SPD Real? You Decide!.


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It is becoming an Easter tradition. For the 3rd time in the last few years we went to Alsace for Easter. Another tradition? It rains whenever we are there. Still the weather mostly held and we enjoyed two big hikes in the Vosges mountains up to 3 huge castle ruins. Imbetween we cycled through wine country.
Click below to enlarge. Lots of old buildings have storks nests. The third picture below is a 15th century tower with a stork on top.


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Your LinkedIn profile picture is your opportunity to let people know that you are happy, confident and approachable. If you post a profile photo that portrays you as negative, threatening or grumpy it is unlikely that people will want to employ you. And you probably won't get the chance to overcome this belief in a job interview.
Research shows that even when exposed to a photograph of a stranger for just 100 milliseconds (or one-tenth of a second), people make judgements on factors including likeability, attractiveness, trustworthiness, competence and aggressiveness.
The first impressions people form can become a self-fulfilling prophecy. They often look for evidence that confirms what they already think so these first impressions tend to remain over time.
Create a positive impression with a LinkedIn profile picture
The eyes have it
Avoid blocking your eyes, for example with sunglasses. This can result in people rating you as less likeable.
Similarly, obstructing your eyes with hair or shadow may make them perceive you as less competent or influential.
Smile and look happy
A LinkedIn profile photo in which the subject is smiling and their teeth are visible has been found to be more than 10% more likeable. For profiles with closed mouth smiles, the impact for likability is about half as much.
But what if you hate your smile? A neutral expression is fine as long as there are no other indicators of negativity in the photo.
This type of expression is much better than a half smile. This is likely to be perceived as showing contempt, superiority or disbelief.
Dress with care
A subjects dressed in formal clothes (eg a dark coloured suit, shirt and tie for men) was rated up to 10% more competent and over 10% more influential than a similar LinkedIn profile picture with the subject dressed in 'neutral' or informal apparel.
Your profession will also influence your choice. Creative types can have more flexibility in their choice of attire. However professionals such as lawyers and accountants should create a solid and trustworthy impression with their clothes.
How much is enough?
Head and shoulders or from the waist up shots are best. Close-ups of the face and full body photos are marked slightly down for likability, competence and influence.
Research shows the background or setting of a LinkedIn profile picture has no statistically significant impact on viewers. However a light-coloured background can be more flattering. If you want to resonate with potential employers, use a physical location or setting that means something to your industry.
Black and white photos have been found to have no significant impact on ratings. Subjects in dark or poorly lit photos are rated slightly lower for likeability.
Trying to make a colour photo look more professional by switching it to black and white can backfire. Avoid unless you know what you're doing with your photo editing software.
Make it look professional
If you take your own LinkedIn profile picture, don't make it look like you took it from your laptop or by holding your arm out. If you can't stretch to using a professional photographer, use the timer setting on your phone or camera. Stand the camera on something solid to take a professionally looking photo.
Avoid placing the camera below your eye level, looking down your nose with your forehead back and chin forward or looking down to see the image. Otherwise the viewer may perceive that you are looking down on them and will consider you as contemptuous or arrogant.
Position the phone or camera at roughly eye level. If you are looking up, you introduce lines in your forehead that could be interpreted as tension or worry. And if the camera angle is significantly above you, you could be perceived as servile or sycophantic.
Hopefully the picture you end up using for your online profile shows you relaxed and comfortable, smiling and projecting positive vibes.
Want help with LinkedIn?
Career coaching can help if you'd like more information on using LinkedIn for job searching or, if you have access to outplacement services, ask your consultant for advice.


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What I saw: "The Light Years," a play by The Debate Society, at Playwright's Horizons, 416 West 42nd Street, in Manhattan, on the south side of the street after the scaffolding ends but before the Hudson River, on that weird off-Broadway strip of theaters I can't keep from confusing with each other.
What I did beforehand: the first year we lived in New York, I thought that coming into the city on a MetroNorth train was like riding an futuristic satellite elevator from an orbiting space station to the surface of the planet. The atmosphere was different. And the gravity. The conductors needed shiny silver suits, of course, but I used my imagination Five years later, I don't feel like a prisoner here as much as I did then. Still, the way the train dives under the streets just south of Harlem means the commuters have to emerge from under the city's skin, like parasites hatching. I brought homemade beer, anyway.
What I wore: Chinese-made Australian boots, new James skinny jeans, black Brooks Brothers fitted cotton blouse, too long Eileen Fisher cardigan, hoop earrings, gold bead necklace, black parka, favorite rag & bone scarf.
Who went with me: The Graduate and his gf S; she liked my jewelry.
How I got tickets: about a week ago, online. They were the last three seat available.
Why I saw this show: it was billed as a "spectacular tribute to man's indomitable spirit of invention."
Where I sat: Row B, Seat 5, next to two unoccupied seats on one side and a woman who laughed too much on the other side. I, also, laughed too much.
Things that were sad: [spoilers]
Things that were funny: lightbulbs, songs, monologues, promises, and a bucket.
Things that were not funny: this one time I was brushing my teeth and I went to put the toothpaste back in the medicine cabinet and got shocked by it. This is the primary memory I have of the place we lived in Salt Lake City in the mid-80s.
Something I ate: a bag of peanuts in the lobby
What it is: an unusual play about the creators of the 12,000-seat theater called The Spectatorium for the Chicago World's Fair of 1893.
Who should see it: electricians, Chicago aficionados, history buffs, aluminum evangelists, love story bugs, theater nerds, devotees of the Depression, bicycle enthusiasts, folding attic stairs fanatics, dirigible fanciers, soliloquy fiends, junk junkies, lovers of lightbulbs, milk maniacs, World's Fair nuts, suckers for jingles, impresario connoisseurs, and anyone who's ever wondered if there's an inventor living in their attic
What I saw on the way home: the ceiling of Grand Central Terminal, with its light-bulb constellations .


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US diplomat apologizes to thousands of Afghans stuck in UAE
ABU DHABI, United Arab Emirates (AP) — A senior U.S. diplomat apologized Friday to thousands of Afghans stranded in the United Arab Emirates months after the fall of Kabul to the Taliban, promising to speed repatriation for some to America while acknowledging that authorities still do not know who some of them are.
The visit comes as America still struggles with how to handle the tens of thousands who piled into planes in the hectic final days of Afghanistan's U.S.-backed government, capping the end of a 20-year experiment in replacing hard-line Taliban rule with a nascent democracy.
While vowing to do all they could, the diplomats who spoke to journalists afterward acknowledged still not being able to answer what would happen to some — and that the journalists, prosecutors and others who made up civil society in Afghanistan might never get U.S. visas. Meanwhile, Emirati officials still must deal with thousands of angry Afghans who already have held protests over the uncertainty.
"I told them that I was really sorry it was taking so long and I was as frustrated as they were, frankly," the senior U.S. official said. "But I also asked for their understanding of how hard we've been working to get the systems going."
The State Department officials all spoke on condition of anonymity to a group of journalists at the U.S. Embassy in Abu Dhabi, citing the "sensitive and ongoing natures of the issues discussed." But the uncertainty over whether some would ever get to the U.S. — and their fear that somehow they could end up back in Afghanistan — remains.
"The problem is nobody knows what's going on," said Ahmad Shah Mohibi, the founder of a group called Rise to Peace, which has been trying to help Afghans stuck there. "The U.S. has a moral obligation" to help them.
Around 12,000 Afghans remain stuck in Abu Dhabi, with some 10,000 at Emirates Humanitarian City and another 2,000 at Tasameem Workers City in the capital, Mohibi said. They represent a cross-section of those who fled the Taliban's lightning advance in August, including journalists, judges, prosecutors, LGBT rights activists and religious and ethnic minorities, he said.
Eight American citizens, as well as three permanent residents, also remain in the housing as they don't want to leave their family members who do not have U.S. visas, Mohibi said. The U.S. officials did not dispute the figures or the descriptions offered by Mohibi.
Perhaps more problematic, some 2,500 former soldiers now in Abu Dhabi identify themselves as belonging to so-called "Zero" Units, Mohibi said.
"Zero" Units — or "Counterterrorism Pursuit Teams" as the Americans called them — had been organized and run by the CIA in the long war against the Taliban. They conducted what Human Rights Watch described as "abusive" nighttime raids that involved extrajudicial killings and airstrikes that sowed anger in Afghanistan.
However, the "Zero" Units served as a bulwark as American and allied forces mounted their chaotic evacuations from Kabul International Airport ahead of the final U.S. withdrawal on Aug. 30.
Asked about the "Zero" Unit personnel in Abu Dhabi, the senior U.S. diplomat said: "Nobody, nobody comes into the United States without full vetting. I don't care who they worked for or what they did or didn't do. That's the best answer I have." However, the vetting that the official described only involves biometric scans and medical checks, not checks on service records.
The official declined to discuss any possible communications with the CIA. The CIA did not immediately respond to a request for comment Friday.
Evacuation flights had been going on until November, but then suddenly stopped, leaving the thousands stranded in Abu Dhabi without any clear route out. Then there are tens of thousands more still stuck in Afghanistan, whom the Taliban initially promised could leave but now may be changing their mind.
The senior official also said that two measles outbreaks among the refugees, concerns over coronavirus vaccinations and later, CDC demands for full medical examinations had slowed down the process of getting Afghans to the U.S.
The official also stressed the UAE controls the two sites in Abu Dhabi and that a full survey of those here still has not been finished. The official also said that private flights approved by the White House in the months after the withdrawal also added more people to the Abu Dhabi facilities.
State media have shown images of Abu Dhabi's powerful crown prince and the country's de facto ruler, Sheikh Mohammed bin Zayed Al Nahyan, visiting the site last year, though visits by journalists have been few.
The senior official also repeatedly praised the Emiratis for their help.
"Frankly, the Emiratis have been generous," Mohibi said. "The problem is the U.S. government is making the UAE look bad. They offered a hand to help … (but now the U.S.) are not helping their ally take the Afghans out."
Also, the Emiratis may be getting concerned. At least two protests have broken out at Emirates Humanitarian City in recent months over Afghans being upset about being stuck. They've been asking for jobs to earn money to send back home and to move on, the senior official said.
But the senior official did choke up when discussing efforts to reunite a child separated from his parents and six siblings in the chaos of the Kabul airport after an Islamic State suicide bomber killed more than 170 Afghans and 13 U.S. service members. The official said she'd like to have refugees in the U.S. by August, but it remained unclear how that could happen.
"But honestly, I can't tell you," she added. "I mean, let me put it this way: The U.S. process is complicated and difficult and time consuming. We are doing our best to speed it up."
Follow Jon Gambrell on Twitter at www.twitter.com/jongambrellAP.


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Wikipedia's Jimmy Wales creates news service Wikitribune
Wikipedia's co-founder Jimmy Wales is planning a news service that combines the work of professional journalists and volunteers. His goal is for Wikitribune to offer "factual and neutral" articles that help combat the problem of "fake news". The service is intended to be both ad-free and free-to-read, so will rely on supporters making regular donations. One expert said it had the potential to become a trusted site, but suggested its influence might be limited. Wikitribune shares many of the features already found in Mr Wales's online encyclopaedia, including the need…Read More


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Everyone knows I've been a bit frustrated with business media coverage, and this morning CNBC put the one and only Donald Trump on for more than just political talk. Trump came on to talk about stocks he he's been buying (which included the much-maligned Bank of America ( BAC )). I figured he would have at least mentioned REITs (as he has said in the past, he only puts his money in what he knows, which is real estate), but that wasn't the case. We all know Donald Trump means ratings, and that is what business television is all about these days.
We heard lots of chatter today that Europe may come together and ban short-selling (traders betting against the market) to stem the recent declines. This is eerily similar to what happened in the U.S. markets prior to the collapse of several financial institutions, as many market observers cried out for investigations into short-selling. In my opinion, all this talk is once again ignoring the true problem at hand: banks using too much leverage and begging for help when their bets go bad. My guess is bailout talk could be next. How will this scenario affect U.S. financial institutions? Well, that remains to be seen.
Before we dig into today's dividend movers, I noticed AOL ( AOL ) shares bounced up 12% today to close at $11.47. The stock had recently plummeted down from $16 following its subpar earnings results. So, what is moving shares back up today? The company announced it intends to buy back $250 million worth of its own stock. This process will reduce the float (number of shares outstanding), but once again, a company is ignoring the true problem at hand. AOL needs to figure out how to grow their revenues and profits of its business instead, either through acquisitions or developing innovative web properties in-house. You can mask weakness easily on Wall Street, but at the end of the day, results are what keep the lights on!
Getting back to the dividend scene today, the markets have taken back part of yesterday's heavy losses. Despite these gains, we are still working through our "Best Dividend Stocks" List and we did remove two names from our list this morning, so be sure to read this post detailing the latest downgrades if you didn't notice the e-mail alert we sent out earlier this morning.
Today's trading was a great time for aggressive investors to get out of high-beta (usually low-yield) plays they may have been getting buried in. As a former trader, it was days like today that I would look to take advantage of higher prices and cut my losses whenever I had positions misplayed.
Earnings results pushed up shares of Cisco Systems ( CSCO ) (still hard to see where new business will come from to bring this former tech titan back), News Corp ( NWS ) (despite the recent troubles the company surprised with results, also raised its dividend but it is still too small to be taken seriously), and Advance Auto Parts ( AAP ) (automakers may want to get out of the manufacturing business and just sell parts!). Wall Street upgrades were also running rampant once again, lifting up shares of Simon Property Group ( SPG ) (REIT yields remain on the low side, and we would love to see a 4-handle in front of the yields), Anadarko Petroleum ( APC ) (with oil prices approaching bear market territory it will be interesting to see if the natural gas stocks can get their mojo back - I'm not so sure about this one), Blackrock ( BLK ) (yield is looking better, but technically asset managers have been breaking down). Another huge batch of Wall Street upgrades comes despite the economic turbulence we are feeling. A negative to possibly take away from today's rally was the fall-off in volume from yesterday's sell-off. We would prefer the opposite effect to be the case, where volume picks up on the big up days and pulls back on lesser volume. As I've been saying, now is not the time to be super-aggressive, although analysts would have you believe differently.
Lastly, gold prices ( GLD ) saw a bit of a pullback as margin requirements got a bit more expensive for traders buying gold futures. Some are saying this could put the brakes on the recent meteoric gold rise, as it was when margin requirements were raised for silver futures.
Just a reminder for all Dividend.com Premium subscribers: with all the recent dividend recommendation changes, unless we specifically issue a "Sell" call on any of the individual names we remove (very rare), it is simply a call to not add any new capital into those particular stocks. The market is clearly undergoing some major turbulence and we want to make sure the names that remain on our list are the best-positioned in a volatile market. That said, some of the names we have removed could find themselves back on the recommended list if we see better entry points or if the worst of the downside is behind us. On that note, be sure to check out our ever-popular weekly "Top 50 Watchlist Names" post that is out today (some recently-downgraded names appear on this list), only for Dividend.com Premium members. Our service isn't about trading, but when the volatility rises, we tend to be more active in our ratings changes. Unlike most of the exuberance and almost always "bullish" Wall Street calls you may be accustomed to, we don't like to take chances with shares that have considerable downside risk. This strategy worked extremely well for us back in 2008/2009 during the financial crisis and subsequent market meltdown, so rest assured, we'll keep you on top of things!
And here's one final note that I like to stress to investors: if you lack consistency when it comes to putting money to work, or are still contemplating your first move, it's time to get busy (regardless of what the market is doing day-to-day, month-to-month, or year-to year). The hardest job isn't finding the right stocks to buy (Dividend.com has that covered), but rather it's the individual's ability to allocate funds and put them to work. Be relentless! No one will ever care more about your money than YOU!
Thanks for reading, and I'll see you tomorrow! P.S. Please pass this e-mail on to someone you think can use some financial motivation as well as being kept in thefinancial newsloop that could affect them.
Be sure to visit our complete recommended list of the Best Dividend Stocks , as well as a detailed explanation of our ratings system here .
The views and opinions expressed herein are the views and opinions of the author and do not necessarily reflect those of Nasdaq, Inc.
Created by Dividend.com
The views and opinions expressed herein are the views and opinions of the author and do not necessarily reflect those of Nasdaq, Inc.


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Eva Gonzales art links
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Art Institute of Chicago NEW!
Musée d'Orsay, ParisArtworks in the museum's collections database
Musée d'Orsay, Paris"Works in focus:" A Box at the Theatre des Italiens
National Gallery of Art, Washington D.C. NEW!Most but not all artists in the NGA database have works online
Bristol Museum and Art Gallery, UKThe Donkey Ride
Minneapolis Institute of Arts, Minnesota Lady with a Fan
Museum Collection Database for the Region of Haute-Normandie, France
National Art Databases and Museum Inventories:Joconde Database of French Museum Collections (in French)
UK National Inventory of Continental European Paintings (NICE)
Christie's Past Sale Archive(database goes as far back as 1991; images go about as far back as 1999)
Sotheby's Sold Lot Archive(database goes as far back as 1998; images where permitted by copyright go about as far back as 2001)
Eva Gonzales at The Art Renewal Center
Eva Gonzales at The Athenaeum 11 works online by Gonzales
Wikimedia Commons Image Database
Eva Gonzales at CGFA
Ciudad de la Pintura (in Spanish)
Olga's Gallery
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The Modernist Journals Project(Click "Retrieve Images" to see artworks)
Women Artists from the Medieval Period to the Present
Union List of Artist Names (Getty Museum) Reference sheet with basic information about the artist and pointers to other references.
Search Amazon for books related to Eva Gonzales
We cannot be responsible for the content of external web sites.All images and text on this Eva Gonzales page are copyright 1999-2015 by John Malyon/Specifica, Inc., unless otherwise noted. Note that the listings on this site are a unique compilation of information and are protected by copyright worldwide.


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Nan Emmett of Spirited Earth. She's contributed one of her handcrafted, ceramic Spirit Rattles. The piece, measuring 4"X3"X2", is hand-painted and makes a musical tapping when you shake it.
Would you like to call this beautiful piece your own?
Please leave a comment on this post for this Spirit Rattle made by Nan Emmett with your bid. If you are the winning bidder, you will receive an invoice after the close of the auction. The auction closes at midnight EST, Wednesday, December 28th! There must be a way to contact you; please leave your email address if your email preferences have been turned off in your profile.
Don't forget, you can also bid on a necklace by me by CLICKING HERE and a necklace by Maria Richmond by CLICK HERE.
And while you're at it... please visit and bid on the pieces by these amazing artists:
Thursday, December 22, 2011
Artists Helping Artists...
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