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Phoenix most often refers to: Phoenix (mythology), a legendary bird from ancient Greek folklore Phoenix, Arizona, a city in the United States Phoenix may also refer to: == Mythology == === Greek mythological figures === Phoenix (son of Amyntor), a Trojan War hero in Greek mythology Phoenix (son of Agenor), a Greek mythological figure Phoenix, a chieftain who came as Guardian of the young Hymenaeus when they joined Dionysus in his campaign against India (see Phoenix (Greek myth)) === Mythical birds called phoenix === Phoenix (mythology), a mythical bird from Egyptian, Greek, Roman and Persian legends Egyptian Bennu Hindu Garuda and Gandabherunda Firebird (Slavic folklore), in Polish Żar-ptak, Russian Zharptitsa, Serbian Žar ptica, and Slovak Vták Ohnivák Tűzmadár, in Hungarian mythology Persian Simurgh, in Arabian Anka, Turkish Zümrüdü Anka, and Georgian Paskunji Chinese Fenghuang, in Japanese Hō-ō, Tibetan Me Byi Karmo, Korean Bonghwang, and Vietnamese Phượng (hoàng) or Phụng (hoàng) East Asian Vermilion Bird in Chinese Zhū Què, Japanese Suzaku, Korean Jujak or Bulsajo, and Vietnamese Chu Tước Chol (bible), Milcham, Avarshina, Urshinah or other transliterations of אורשינה Nine-headed Bird, one of the earliest forms of the Chinese phoenix (Fenghuang) == Places == === Canada === Phoenix, Alberta Phoenix, British Columbia === United States === Phenix City, Alabama Phoenix, Arizona Phoenix metropolitan area, Arizona Phoenix, Georgia Phoenix, Illinois Phoenix, Louisiana Phoenix, Maryland Phoenix, Michigan Phoenix, Mississippi Phoenix, Edison, New Jersey Phoenix, Sayreville, New Jersey Phoenix, New York Phoenix, Oregon === Elsewhere === Phoenix (Caria), a town of ancient Caria, now in Turkey Phoenix (Crete), a town of ancient Crete mentioned in the Bible Phoenix (Lycia), a town of ancient Lycia, now in Turkey Phoenix Park, Dublin, Ireland Phoenix Islands, in the Republic of Kiribati Phoenix, KwaZulu-Natal, in South Africa Phoenix City, a nickname for Warsaw, the capital of Poland Phoenix, a river of Thessaly, Greece, that flowed at the ancient city of Anthela == Arts and entertainment == === Fictional entities === ==== Characters ==== Phoenix (comics), alias used by several comics characters Phoenix Force (comics), a Marvel Comics entity Jean Grey, also known as Phoenix and Dark Phoenix, an X-Men character Rachel Summers, a Marvel Comics character also known as Phoenix Phoenix (Transformers) Phoenix Hathaway, a character in the British soap opera Hollyoaks Phoenix Raynor, a Shortland Street character Phoenix Wright, an Ace Attorney character Aster Phoenix (or Edo Phoenix), a Yu-Gi-Oh! GX character Paul Phoenix (Tekken), a Tekken character Simon Phoenix, a Demolition Man character Stefano DiMera, also known as The Phoenix, a Days of our Lives character Phoenix, female protagonist of the film Phantom of the Paradise, played by Jessica Harper Phoenix Buchanan, a fictional actor and the main antagonist of Paddington 2 Phoenix Jackson, female protagonist of "A Worn Path" by Eudora Welty ==== Organizations ==== Phoenix Foundation (MacGyver) Phoenix Organization, an organization in John Doe Order of the Phoenix (fictional organisation), a secret society in Harry Potter ==== Vessels ==== Phoenix (Star Trek), a spacecraft === Film === Fushichō (English: Phoenix), a 1947 film by Keisuke Kinoshita The Phoenix (1959 film), by Robert Aldrich Phoenix (1998 film), a crime film by Danny Cannon Phoenix (2006 film), a gay-related film by Michael Akers Phoenix (2014 film), a film by Christian Petzold Phoenix (2023 film), a Malayalam film === Literature === ==== Books ==== Phoenix: The Posthumous Papers of D. H. Lawrence (1885–1930), an anthology of work by D. H. Lawrence Phoenix (novel), by Stephen Brust The Phoenix (novel), by Henning Boëtius Phoenix IV: The History of the Videogame Industry, by Leonard Herman ==== Comics ==== Phoenix (manga) (Hi no Tori), by Osamu Tezuka The Phoenix (comics), a weekly British comics anthology ==== Periodicals ==== The Phoenix (magazine), Ireland The Phoenix (newspaper), United States' Phoenix (classics journal), originally The Phoenix, a journal of the Classical Association of Canada Project Phoenix, codename of the aborted BBC Newsbrief magazine List of periodicals named Phoenix ==== Other literature ==== The Phoenix (play), by Thomas Middleton The Phoenix (Old English poem) The Phoenix, a play by Morgan Spurlock The Phoenix, a poem attributed to Lactantius === Music === ==== Musicians ==== Phoenix (band), a French alternative rock band Transsylvania Phoenix, also known as Phoenix, a Romanian rock band Dave Farrell, American bass guitarist in the band Linkin Park ==== Albums ==== Phoenix (Agathodaimon album) Phoenix (Asia album) Phoenix (Vince Bell album) Phoenix, a 2003 EP by Breaking Pangaea Phoenix (Charlotte Cardin album) Phoenix (Carpark North album) The Phoenix (CKY album) Phoenix (Clan of Xymox album) Phoenix (Classic Crime album) Phoenix (Dreamtale album) Phoenix (Emil Bulls album) Phoenix (Everything in Slow Motion album) The Phoenix (EP), an EP by Flipsyde Phoenix (Dan Fogelberg album) Phoenix (Grand Funk Railroad album) Phoenix: The Very Best of InMe, a 2010 greatest hits collection The Phoenix (Lyfe Jennings album) Phoenix (Just Surrender album) Phoenix (Labelle album) The Phoenix (Mastercastle album) Phoenix (Nocturnal Rites album) Phoenix (Rita Ora album) Phoenix, an album by Pink Turns Blue The Phoenix (Raghav album) Phoenix (Warlocks album) Phoenix (EP), by the Warlocks Phoenix (Zebrahead album) === Songs === List of songs named for the phoenix === Television === The Phoenix (1982 TV series), an American science fiction series Phoenix (Australian TV series), an Australian police drama Phoenix (South Korean TV series), a 2004 Korean drama Phoenix (anime), a 2004 Japanese series based on the manga "Phoenix", the 1986 premiere episode of The Adventures of the Galaxy Rangers "The Phoenix", a 1995 episode of Lois & Clark: The New Adventures of Superman "Phoenix", a 2003 episode of Smallville "Phoenix" (Breaking Bad), a 2009 episode of Breaking Bad "Phoenix" (NCIS), a 2012 episode of NCIS === Video gaming === Phoenix (1980 video game), a shoot 'em up arcade game Phoenix (1987 video game), a space combat simulation developed by ERE Informatique Phoenix Games (American company), a video game company Phoenix1, a League of Legends team === Other uses in arts and entertainment === Atlanta from the Ashes (The Phoenix), an Atlanta, Georgia, monument Phoenix Art Museum, the Southwest United States' largest art museum for visual art Phoenix (chess), a fairy chess piece Phoenix (roller coaster) Phoenix, a Looping Starship ride at Busch Gardens Tampa Bay == Business == In business, generally: Phoenix company, a commercial entity which has emerged from the collapse of another through insolvency Specific businesses named "Phoenix" include: === Airlines === Phoenix Air, an airline operating from Georgia, United States Phoenix Aviation, a UAE-Kyrgyzstan airline === Finance companies === The Phoenix Companies, a Hartford-based financial services company Phoenix Finance, a financial company which attempted to enter into Formula One racing Phoenix Fire Office, a former British insurance company === Media companies === Phoenix (German TV station) Phoenix (St.


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subset: commoncrawl


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Darryl M. White is a former vice president for advancement for the Mackinac Center for Public Policy. In this role, White led the Center's fundraising efforts by coordinating relations with individual contributors, organizing events and managing grant requests to foundations and businesses.
White has extensive experience in the management of nonprofit organizations, particularly in efforts to address social needs through the voluntary actions of private citizens. For 12 years, White was executive director of the Voluntary Action Center of Midland County, where he oversaw the recruitment of thousands of volunteers on behalf of nonprofit agencies in the area. White has also been extensively involved in private-sector workforce development: He served first as a consultant to, and later as executive director of, MITECH+ Inc., and he worked for two years as executive director of the Michigan Center for Construction Education and Research, a nonprofit founded by the Saginaw Valley Chapter of Associated Builders and Contractors.
In addition, White served for seven years as executive director of the Pregnancy Resource Center of Mid-Michigan, where he was responsible for fundraising, program development and facility management. Prior to joining the Mackinac Center in January 2010, White was president and founder of a successful, privately held firm that specialized in consulting, training, career counseling and human resources support services.
White holds a master's degree in public administration from Central Michigan University and a bachelor's degree in sociology from the University of Wisconsin at Stevens Point. He lives with his wife, Julie, and his two children in Midland, Mich.


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I got to try out one of these bad boys while I was in Arizona - my mom has a Bernina dealer within walking distance of her house! (The cruel irony is that she isn't interested in Berninas at all; she's a Pfaff girl.) I absolutely loved using this machine.
Aside from the quality of the stitches, what I really liked was how intuitive it felt to use. I could see myself adapting quickly to this machine.


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The two men rushed through the glass doors of Chiefs headquarters and came face-to-face with linebacker Jovan Belcher, holding a handgun to his head.
Belcher had already killed his girlfriend and sped the short distance to Arrowhead Stadium, right past a security checkpoint guarding the entrance. Upon finding his bosses, Belcher thanked general manager Scott Pioli and head coach Romeo Crennel for giving him a chance in the NFL. Then he turned away and pulled the trigger.
The murder-suicide shocked a franchise that has been dealing with controversies now made trivial by comparison: eight consecutive losses, injuries too numerous to count, discontent among fans and the prospect that Pioli and Crennel could be fired at season's end.
Authorities did not release a possible motive while piecing together the case, other than to note that Belcher and his girlfriend, 22-year-old Kasandra M. Perkins, had been arguing frequently.
The two of them left behind a 3-month-old girl. She was being cared for by family.
The Chiefs issued a statement that said their game Sunday afternoon against the Carolina Panthers would go on as scheduled, even as the franchise tried to come to grips with the awfulness of Belcher's death.
"The entire Chiefs family is deeply saddened by today's events, and our collective hearts are heavy with sympathy, thoughts and prayers for the families and friends affected by this unthinkable tragedy," Chiefs chairman Clark Hunt said in brief a statement.
A spokesman for the team told The Associated Press that Crennel plans to coach on Sunday.
"I can tell you that you have absolutely no idea what it's like to see someone kill themselves," said Kansas City Mayor Sly James, who spoke to Pioli shortly after the shootings.
"You can take your worst nightmare and put someone you know and love in that situation, and give them a gun and stand three feet away and watch them kill themselves. That's what it's like," James said. "It's unfathomable."
The 25-year-old Belcher was from West Babylon, N.Y., and played college football at Maine. He signed with the Chiefs as an undrafted free agent, made the team and hung around the past four years, eventually moving into the starting lineup. He played in all 11 games this season.
The NFL released a statement expressing sympathy and pledging "to provide assistance in any way that we can." The players' association has also been in touch with members of the Chiefs.
"We sincerely appreciate the expressions of sympathy and support we have received from so many in the Kansas City and NFL communities, and ask for continued prayers for the loved ones of those impacted," Hunt said. "We will continue to fully cooperate with the authorities and work to ensure that the appropriate counseling resources are available to all members of the organization."
The drama unfolded early Saturday when authorities received a call from a woman who said her daughter had been shot multiple times at a residence about five miles from the Arrowhead complex. The call came from Belcher's mother, who referred to the victim as her daughter.
"She treated Kasandra like a daughter," Kansas City police spokesman Darin Snapp said, adding that the women had recently moved in with the couple, "probably to help out with the baby."
Police then got a phone call from the Chiefs' training facility, and Belcher's description matched the suspect description from the initial address. Snapp said officers pulled into the practice facility parking lot in a matter of minutes, in time to witness the suicide.
"Pioli and Crennel and another coach or employee was standing outside and appeared to be talking to him," Snapp said. "The suspect began to walk in the opposite direction of the coaches and the officers and that's when they heard the gunshot. It appears he took his own life."
The coaches told police they never felt in any danger.
"They said the player was actually thanking them for everything they'd done for him," Snapp said. "He was thanking them and everything. That's when he walked away and shot himself."
Members of the Chiefs laid low Saturday, but a few of them reacted on Twitter.
"I am devastated by this mornings events," Pro Bowl linebacker Tamba Hali wrote. "I want to send my thoughts and prayers out to everyone effected by this tragedy."
A large group of Belcher's friends and relatives gathered Saturday at his boyhood home on Long Island.
His family turned the front yard into a shrine, with a large poster of Belcher, an array of his trophies, and jerseys and jackets from Kansas City, Maine and West Babylon High.
"He was a good, good person ... a family man. A loving guy," said family friend Ruben Marshall, who said he coached Belcher in youth football. "You couldn't be around a better person."
At least 20 people gathered for a large group hug in the driveway.
"He was a tremendous player and all those things, and his accolades speak for themselves, but he lit up when he spoke about his mom, or when he hugged his family after games," said Dwayne Wilmot, who was Belcher's position coach at Maine and is now an assistant coach at Yale.
"It's difficult to talk about Jovan in the past tense," he told the AP. "There's going to be unanswered questions, the why's of this tragedy. It'll never be truly known to us."
Wilmot said he'd stayed in touch with Belcher the past few years through social media.
"He was someone who took genuine pleasure in bringing happiness to others," Wilmot said. "I was so excited when he became a father, because I knew he'd be a great father."
His girlfriend's Facebook page shows the couple smiling and holding the baby.
Belcher is the latest among several players and NFL retirees to die from self-inflicted gunshot wounds during the past few years. The death of star linebacker Junior Seau, who shot himself in the chest in at his California home last May, sent shockwaves around the league.
Seau's family, like those of other suicide victims, donated his brain tissue to medical authorities to determine if head injuries he sustained playing football might be linked to his death. That report has not been released, although an autopsy showed no underlying hemorrhaging or bruises on Seau's brain.
Belcher did not have an extensive injury history, though he was listed as having a head injury on a report from Nov. 11, 2009. Belcher played four days later against the Oakland Raiders.
Earlier this year, the NFL provided a grant to help establish an independently operated phone service that connects players, coaches, team officials and other staff with counselors trained to work through personal and emotional crises. The NFL Life Line is available 24 hours a day.
The season has been a massive disappointment for the Chiefs, who were expected to contend for the AFC West title. They're 1-10 and mired in an eight-game skid marked by injuries, poor play and fan upheaval. During the past few weeks there have been constant calls for Pioli and Crennel to be fired.
It's unknown how the Chiefs plan to pay tribute to Belcher during Sunday's game.
"His move to the NFL was in keeping with his dreams," said Jack Cosgrove, who coached Belcher at Maine. "This is an indescribably horrible tragedy."
Associated Press Writers Heather Hollingsworth and Frank Eltman contributed to this report.


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Janet B. Matsen:Lab Tips & Tricks
Back to Janet
I came to the Lidstrom Lab with zero molecular biology or synthetic biology experience. I have great appreciation for all of the resources put online by the OpenWetWare community and other websites, so I am hopeful that I can share some of what I have learned. Please contact me with questions, comments, and corrections.
- TB (terrific broth) gives you healthier cells and more plasmids than LB. I use LB for freezer stocks because or media prep person makes it in abundance but TB for minipreps to get higher plasmid yield.
- SOB media ("super optimal broth") can be used like TB. The recipes are somewhat variable.
- SOC media is SOB with added glucose; it can be used when cells recover from transformation.
- Our lab and Rob Egbert haven't needed to use it as of 2012/10/3. But I am considering making some SOC for electroporation of Gibson assemblies.
- Adam Wargacki claims he recovers 2-5 times more colonies with SOC. They buy commercially prepared competent cells. As he moves to using less and less of the aliquots per transformation, he prefers to use SOC. He also suggests SOC for difficult to transform plasmids, such as 30kb+ plasmids, and even prolonging the recovery time to more than 1 hour. However, if you expect the transformation to work, there isn't much advantage over LB.
Pouring Plates
- Before melting agarose to pour, put it in a heater that keeps agar liquified for a few hours. This makes it melt much faster so you won't have to babysit it.
- Once melted, put it in an empty styrafoam shipping box so it won't develop solidified chunks. You can leave the lid off or leave it partly on to slow down the cooling.
Sterile Technique
Keep an extra clean set up pipette tips
- I find it useful to keep a super fresh box in the drawer that I only pull out for occasions when sterility is critical. When the less sterile tips on my bench are used up, I remove the sterile sticker from a drawer box and move it to the countertop.
- I like that it is inside my bench so nobody can accidentally open it under non-sterile conditions.
- If the not-so-sterile tip boxes accumulate, it is ok to autoclave our brand.
- Alert: Autoclaving (on the dry cycle) loosened the filters so they are not sufficiently held in place. Turning a tip upside down is sufficient to release a tip after autoclaving.
- Additionally, Andrew said they always autoclave filters used on the bioreactor in the wet cycle to prevent damage to the filter.
- I haven't tried to autoclave on the wet cycle yet (3/27/2013), but that may be more useful.
- They DO melt in the waste autoclave cycle (Casey the dishwasher said so 3/2013)
Contamination on plates
- Don't be surprised if you get contamination on plates with multiple antibiotic resistances. There are plenty of plasmids floating around natural systems with multiple on them already. Bacteria make antibiotics in natural systems to add competition to an an environment. (Mary Lidstrom, 2012)
Numbering for Experiments
- I find numbers more useful than labels for most of my experiments. Keep a master google doc spreadsheet. With these, you can do better documentation than a lab notebook and elaborate tape labels allow. example spreadsheet, example commentary for corresponding spreadsheet.
- This also saves time with labeling, and reduces the risk that you will mistake one sample for something with a similar name. It also provides an order for your samples at each step of handling. You can label plates as you make them, cultures as you inoculate them, etc.
- I can't stand the feeling of being confused about something I did experimentally in the past, and this is my best solution.
DNA Magic
Grow in TB, not LB
- This alone gives me order(s) of magnitude higher miniprep yields.
Grow overnight cultures with sufficient head space
- the air volume should be at least 4 times the liquid volume.
- increasing the culture volume seems like a good idea at first but the lower oxygen concentrations are detrimental to plasmid yields.
- Specific suggestions for amounts of culture grow pSB plasmids overnight before miniprepping:
- pSB1A3 (high copy number): 2mL of TB is sufficient for miniprepping. 5 mL in a 15 mL test tube is great.
- pSB3K3 (medium copy number): 5+ mL in green-capped test tubes (they are 25+ mL)
- pSB4C5: I have only done pSB4C5 (low copy number) once I prepped from 50 mL TB (overnight in a 250 mL flask) and got a very pleasing 200 ng/uL in 50 uL. The absorbance at 230nm looked higher than usual but the DNA peak was good. Now I usually do 5-10 mL in a green-capped tube, but have never gotten 200 ng/uL!
The strain you use really matters!
- I do most of my minipreps from Top10 strains. If you use other strains, make sure they are cloning strains. Cloning strains have nucleases knocked out, making your miniprep more stable.
- In 10/2012 I tried miniprepping DNA from BL21(ai) and doing a restriction digest. The DNA was totally degraded afterward. I compared the phenotypes of Top10 and BL21(ai) using this list and found that Top10 has EndA1 knocked out, whereas BL21(ai) didn't.
- Elizabeth "Betsy" Skovran taught Amanda to use PB buffer. The composition is proprietary. We use this on our Thermo Scientific miniprep kit: add 500 uL after loading DNA.
- Optimizing PCR rxns:
- Try the PCR with 55oC for annealing and 0% DMSO first, almost always. If that doesn't work, you will see the manual gives you MANY options for things to change including template concentration, primer concentration, annealing temperature, Mg2+ concentrations, polymerase concentration, alternate buffers, extension time, and annealing temperature. There is no way you could test all the combinatorial possibilities here. I generally only change the annealing temperature and % DMSO if my template concentration is within the suggested range.
- When Tm = 55oC failed, I used to do 12 rxns (20 uL each) with 4 temperatures (on a gradient PCR block), each at 4 temperatures ranging from 55oC - 70oC, and 0%, 5%, and 10% DMSO. If one of these conditions don't work, there is little hope that changing other things will be a game changer. Now that feels like too much work & I only do a few temps w/o DMSO.
- Try the PCR with 55oC for annealing and 0% DMSO first, almost always. If that doesn't work, you will see the manual gives you MANY options for things to change including template concentration, primer concentration, annealing temperature, Mg2+ concentrations, polymerase concentration, alternate buffers, extension time, and annealing temperature. There is no way you could test all the combinatorial possibilities here. I generally only change the annealing temperature and % DMSO if my template concentration is within the suggested range.
- If you are unsure whether you added an ingredient to the PCR mix, add it again.
- I *always* have a written list of components and the volume I want to add and I pen in a dot next to ingredients I have added. However, it is possible that you can get distracted and be unsure whether you added a component. If that component is template, nucleotides, or enzyme, I always add it again. I have never been unsure about whether I added buffer or DMSO (when desired) so I can't promise performance if you double these concentrations.
- I also add the most abundant & inexpensive components to the mix first. Thus, I add water, buffer, then primers. If I am unsure that something is wrong at this point, I can scrap it without any lost of scarce or valuable materials.
- If making more than one master mix, label the tubes of master mix by writing the number of the primers on the lid. Then you can easily double check that the right primer goes in the right mix as you create it.
- I don't actually do this because I like only having 1 kit. Keeps things simple.
- Annealing @ 55oC in PCR before anything else often works great. You are more likely to get undesired bands due to mis-priming but it is often a great place to start.
- You can re-use tips when loading into wells if you rinse in the TB buffer in your block between every step. This is great because tips are ~$40/box. Don't do this for important samples (just in case.)
- If you are gel-purifying large volumes of PCR product, you can tape the lanes together. The greatest dangers are (a) the well ripping and allowing your sample to leak out and (b) having the buffer slosh you sample out of the well when you move or bump the box. Come talk to me about extremes in taping because I have made all the possible mistakes.
- I don't actually do this any more (2012/07/02) because I am using Gibson cloning and don't need much DNA any more.
- Running large volumes on a gel ("overloading" the gel) can cause the band size to run too fast or too slow. If you are running a large amount for purification, include a lane next to the ladder that is not overloaded so you can be confident you know the band size. The following gel ran alright, but a slight shift is visible.
- If you have a voltage source that will stop after a set amount of time, feel free to run your gel as you leave and image it in the morning. Diffusion of bands shouldn't be a big problem unless they are << 500 bp.
- Don't do this if you are going to cut & gel purify the band.
Purifying DNA
- purifying DNA on a gel column has ~ 90% loss. purifying DNA on a column (non-gel) has ~ 90% recovery. avoid gel purification. (Justin)
- Freeze 'N Squeeze Gel extraction may be worth a try.
- I'd like to try. I even have enough of a kit to try some, but I never have samples I feel like taking the risk on! The only person I know who does it (also the person who gave me a piece of a kit) swears by it.
- Purify gels on gel-specific kits. Purify PCR products on PCR product specific kits if you have one. The former does a less effective job removing primer dimers. (Justin)
Eluting DNA from miniprep & gel extraction columns
- By default, most people elute from miniprep and gel purification columns in water instead of the supplied buffer. This is motivated by the desire to have DNA that is free of buffers and other compounds that may inhibit subsequent reactions (e.g. PCR or Gibson Assembly). You may, however, suffer from lower recovery of DNA from your column as the pH of the water you use is likely < 7.5.
- Both the Qiagen gel extraction kit and Thermo Scientific miniprep kit use 10 mM Tris·Cl , pH 8.5 elution buffer.
- Should not inhibit PCR. (No EDTA, a chelator)
- Gibson assembly mix is ~ 200 mM Tris, pH 7.5. Adding a few uL of 10 mM pH 8.5 Tris shouldn't have a huge effect. I'd like to call their tech support and make sure, however.
- Is ligation the main issue? I'm not doing a lot of restriction cloning right now, so this wouldn't be a huge issue for me. (10/2012)
Rebound board for ergonomic pipette dispoal
- Check out my rebound board for discarding used tips! I won't be shooting tips onto Amanda's bench any more.
Ice Buckets
- the best type of ice bucket we have in lab are the ones that perishable reagents are delivered in when mailed. These are more insulating and have excellent lids. They are also useful to use when flash freezing samples with liquid nitrogen. I have no idea why nobody else has these in their starting lineup.
An ode to scotch and boxing tape
- Masking tape and boxing tape are extremely great when laid on top of markings on your tubes. This will make your labeling more resilient to ethanol exposure and mechanical wear. It helps preserve your writing when your rub off ice that accumulates on the top when tubes are stored at -80oC. I cover any labeled tube that I will be keeping for a while with tape.
- Warning: I have gotten eppendorf tubes really stuck in a centrifuge before when I use cryogenic labels and overlaid them with boxing tape. I was able to get them out easily without working hard by putting the rotor in a 60oC oven for a while before pulling on the tubes with plyers, but this should be avoided.
When plating transformations you expect will work
- If plating a transformation of miniprepped DNA, you can do two things to save time/mess:
- Plate with wooden spreading sticks
- If you expect decent efficiency, there is no need to use roller beads, a flame sterilized stick, or any other extra tool.
- Just use a pipette tip
- Move the cells to the plate with a pipette tip (works for ~20 uL, but may not be as great for 200 uL)
- bend the pipette tip by pressing it carefully against the sterile side of the plate lid
- Spread cells
Nonunifrom plating is good for miniprepped plasmids you expect to transform with reasonable efficiency
- I like nonuniform spreading because it will allow you to grab single colonies even if your transformation efficiency is really high or really low.
- If the efficiency is high and you plate it nice and evenly, you can have a lawn across the whole plate making it hard to pick single colonies. If the efficiency is low, you don't need to distribute it evenly anyway because a handful of colonies in a relatively small area of a plate will still give you single colonies you can pick.
- I plate gibson assemblies (usually low efficiency) with beads.
Outgrowth over the weekend is not recommended
- Don't transform plates at room temp over the weekend, especially if you rely on Amp resistance. Often you see a lot of contamination!
Electroporation is MUCH MUCH more efficient than chemical transformation
- Transformations done with 1uL of 0.1 ng/uL DNA (pGA 3K3 RFP from Rob Egbert). 0.1 ng is ~10^8 plasmid copies. Tami's electrocomp cells yielded several hundred colonies, which suggests the transformation efficiency is on the order of 100/10^8 = 0.000001 = 0.0001% efficiency. The chemically competent cells yielded < 10 colonies, which is much lower efficiency.
Make your electrocompetent cells super viscous
- Really concentrated cells perform better; my electrocompetent cells are so viscous they barely pipette. The picture below is with more dilute electrocompetent cells that I currently (8/2013) use but illustrates the importance of concentrated cells.
- My cells clump into a "booger" during recovery (pre-plating) that is mostly unspreadable. The clumping is less of an issue if you don't centrifuge them before plating. For this reason I recover in 200 uL and plate all of it.
Keeping primers, plasmids, strain stocks, & sequencing organized
- Keep a master google spreadsheet with a page each for primers, plasmids, strain stocks, and sequencing. (example) This will keep you very organized, and allow you to collaborate more easily.
- On the primers page, each primer should have an individual number, a name without spaces, the sequence, comments for its intended use, and the date you ordered it. When ordering primers from IDT, you can paste the three consecutive columns with primer number, name, & sequence into the multiple primer entry window. Then delete the space between the primer number & name, and add a hyphen. You can grey out primers you decide are no longer useful (example: you find out it is wrong or doesn't work).
- On the plasmids page...
- On the strain stocks page...
- Keep the primers, plasmid stocks, cell stocks, and strain stocks in boxes with their unique identification number on top. Order them by number. This allows for ease of sharing resources & information. Note that the plasmids can be stored as minipreps and in the cloning strains, so you will have two boxes that share the same numbers.
- Keep a dropbox folder with the final versions of all of the plasmids you trust & keep.
- I name them by their plasmid number & short description.
- In this, I keep an extra copy of the sequence file that is annotate with all of the confirming sequencing rxns I did. For each sequence that comes back, I add a feature that is named with the order number of the sequencing reaction & the primer that was used to obtain the sequence. The feature should cover only the part of the sequence for which the sequencing reaction was solid. This means visually trimming off areas with N's.
pH depends on dilution of a buffer and presence of salts
I wrote two sections here:
- Why does the pH of a buffer change with dilution?
- Why does the pH of a buffer change with the addition of salts (changes in ionic strength)?
Using BLAST
- Useful intro for beginners and intermediate users: Chapter 16: The BLAST Sequence Analysis Tool
- Alignment metrics:
Metric Abbreviation | Metric | Basic Description |
bit score | bit score | calculated from a formula that takes into account the alignment of similar or identical residues, as well as any gaps introduced to align the sequences. Can be compared across searches (it is normalized). |
E-value | expect value | indication of the statistical significance of a given pairwise alignment and reflects the size of the database and the scoring system used. The lower the E-value, the more significant the hit. A sequence alignment that has an E-value of 0.05 means that this similarity has a 5 in 100 (1 in 20) chance of occurring by chance alone. Might not be biologically significant though! |
- Legend for alignments:
Alignment Feature | Description |
plus | Conservative substitutions (that is, the substitution of amino acids whose side chains have similar biochemical properties), as judged by the substitution matrix, are indicated with +. Note: it doesn't stick to the groupings we are taught in biochem. I have seen Q (-ONH2: polar, uncharged) paired with K (NH3+: charged), Q paired w/ E (- charged COO-), N (uncharged -ONH2) paired with + charged H (N ring), etc. Haven't found anything that summarizes what they consider "conservative". 9/24/2013. Is it only separating hydrophobic from all others? No. D and P aren't marked w/ a +. |
dashes | insertions or deletions |
lowercase gray letters | low complexity |
X | Amino acid residues in the query sequence that have been masked because of low complexity are replaced by Xs |
Uncertainty Codes: As you work with nucleic acid sequence records and read scientific articles, you may come across representations of bases which are not A, T, G, or C. In fact there are several of these in Figure 3.3. These nonstandard letters are actually the uncertainty codes proposed by the International Union of Biochemistry and Molecular Biology (IUBMB) to represent certain groupings of nucleotides. On occasion, software or a scientist is unable to decide if a newly sequenced base is, for example, an A or a G. The three IUBMB symbols most frequently encountered are R for the puRines (A or G), Y for the pYrimidines (C or T), and N for aNy nucleotide. Here are the uncertainty codes:
IUBMB symbol | Definition |
R | A or G |
Y | C or T |
K | G or T |
M | A or C |
W | A or T |
S | C or G |
B | C or G or T |
D | A or G or T |
V | A or C or G |
H | A or C or T |
N | G or A or T or C |
Using APE (free plasmid editing software)
- Pros:
- Free --> can have it on all of your computers
- Allows annotation libraries --> Easy annotation of new plasmids
- About the annotation libraries:
- This is one of the most useful tools. You can edit them in any text editor. To use them, use the drop-down menus to load the library, then click to annotate your current sequence using this library.
- I keep an annotation library that holds all of my sequencing primers, and use this to sequence clones. I open my plasmid annotated with genes, etc., then load this library of primers and overlay the primers on top of my sequence. If you have annotations of the regions of your plasmid that you have sequence verification for, it makes it very easy to find primers that will sequence other regions you are interested in.
- Beware:
- When you are searching for a sequence in your APE file, it is possible that it will incorrectly tell you it is not there
- This is happening because the DNA is stored a linear, not circular. If you have the cursor clicked mid-way through the file, the "find next" button will only look in the portion of the sequence that is between your cursor and the last letter. This can be avoided by storing all DNA as circular. There is a rectangular button in the upper right corner you can click until it says "circular."
- The alignment tool has given me ... unusual... results. Instead, I mostly use BLAST and use the blastn option.
- Note: FASTA format needs to have a space after the >
- When you are searching for a sequence in your APE file, it is possible that it will incorrectly tell you it is not there
- Mendeley : reference/paper manager that's awesome
- Janet's workflow: (1) discover papers via google scholar saved search, (2) save them to Mendeley in the appropriate nested folder regardless of whether you intend to read it now or "some day when I'm thinking about that topic" (3) read in Mendeley, which makes a green dot (indicating unread) go away.
- You can highlight and keep notes on top of your PDFs enabling quicker review of topics.
- Imagine being able to query all of the papers you have ever read for a topic of interest! You can also see whether you have ever read a paper or not, potentially saving yourself time.
- I also like the idea that I will never lose track of a paper I want to tell someone about. It isn't uncommon for someone to tell me about a paper but be unable to find it and send it to me. This should never happen for a dedicated Mendeley user.
- I'm not thrilled with the word plug-in for inserting references.
Shipping Samples
Shipping samples containing proteins on dry ice
- Beware that shipping samples on dry ice (solid CO2) can affect the pH of the sample. It probably doesn't matter for RNA seq samples, but I know some of us send samples containing protein on dry ice from time to time.
- evidence: http://www.nature.com/nmeth/journal/v10/n4/pdf/nmeth.2409.pdf
- "Proteins generally exhibit low solubility near their isoelectric point (pI). Therefore, acidic proteins have an increased tendency to aggregate or precipitate as pH falls below physiological levels."
- Interestingly, a pH change did not occur when they stored the samples in a -70oC freezer for 72 hours. The CO2 had time to dissipate. Note the tubes are CO2 permeable!
- evidence: http://www.nature.com/nmeth/journal/v10/n4/pdf/nmeth.2409.pdf
Tech Support
I have a lot of questions, always. The longer I work with a piece of equipment or technique, the more I get into the nitty gritty. Thus it is important for me to have good resources to answer the questions I come up with.
- It is incredible that in this day and age, manuals aren't available online for some manufacturers.
- 1/2013: I'm trying to use some of the manual integration features on a Shimadzu HPLC. These features aren't addressed in the manual. They don't make any of their manuals available online!
- If you can, buy an Agilent HPLC instead!
- 1/2013: I'm trying to use some of the manual integration features on a Shimadzu HPLC. These features aren't addressed in the manual. They don't make any of their manuals available online!
- I have the best things to say about BioRad and Agilent and would happily buy their products.
- Though they may not be the least expensive, but they make their manuals available on line and have lots of publications and tutorials for the basics relevant to their equipment.
- Biorad has excellent technical support. I have always been connected to helpful and insightful PhD holding individuals who have firsthand experience with the methods and tools I'm using.


text:
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Carolyn Kizer was a force—not just in the U.S. or Northwest poetry scene, but as a total whirlwind presence of a person. Wearing her mink and a deep scarlet lipstick, she'd swoop into a roomful of people and swirl everyone under her spell. She called me Dahhling. Dahhling, could you find me an ashtray? She almost always had a cigarette in hand, which she used to punctuate sentences. A fellow Spokanite, she seemed to like returning to our town, a place with which she often said she had a love-hate relationship. "Some people from Spokane are insane salesmen / Peddling encyclopedias from door to door," continues to be a favorite, crazy-true-to-this-day line of hers. I think her translations (many of which may be found in Carrying Over) are not nearly as well known as they should be. She had one of the most amazingly gorgeous and powerful reading styles/voices of anyone I've ever heard: that force revved up and unleashed on stage. Her poetry performances were more often recitations than readings. I'll never forget her; she was truly a grande dame. Nance Van Winckel
I have often returned to Carolyn Kizer's poem "Seasons of Lovers and Assassins," which although dated June 5, 1968 (the date of Bobby Kennedy's assassination) seems more articulate about the past ten years' upheavals than most poems directly addressing 9/11, Hurricane Katrina, the wars in Iraq and Afghanistan, and other overwhelming and bewilderment moments that have, as in Kizer's poem, left us "to our fate,/ Wrapt in a caul of vulnerability." The poem begins with lovers "safe from the wild storms off Cape Hatteras," making their own storm on the beach, like Deborah Kerr and Burt Lancaster in From Here To Eternity. In an echo of her teacher, Theodore Roethke, they "swim to sleep, and drown." After they dream, "another ocean wakes" them: news of the assassination. From here on, the sound of the poem rises to a bare, precise, majestic clarity:
Numbly we move to the noontime of our love:
The strip of rain-pocked shore gleams pallidly.
Fragments of broken palm-front fly like knives
Through tropic wind. Soon we bear star-shaped wounds,
Stigmata of all passion-driven lives
Marked lovers now, the moony night is ours,
Surf-sounds reminding us that good decay
Surrounds us: force which pounds on flesh or stone,
The slow assassination of the years.
Kizer has a public voice, one that hears its audience, like Pope. Her work is agile and present, never miserable, lonely, oracular, or visionary, but even in her essay-precise oratory she acknowledges the counter-poetry, listens to it (albeit with a coldness, a Chekhovian coldness that brings me closer as a reader). She is at once the poet she is, the lauded Spokane Augustan—and the poet she isn't, the one from "To An Unknown Poet," who, while the speaker is getting "an award/ from the American Academy/ and Institute of Arts and Letters" in a "noble old building,/ as pale as the Acropolis/ awash in a sea of slums," is "far away, on the other coast,/ as far from our thoughts as Rimbaud/ with his boy's face and broken teeth."
I admire Kizer's poems and career, how she has been able to speak to the importance of poetry, and act on it, helping found this magazine and the NEA, all very reputable activities, and yet in her work has never strayed far from the rude, rough mysteries of the imagination. Ed Skoog
Some years ago, at a rather swank party, I was shocked to overhear the hostess refer to someone as "a good bitch." It took me a moment to realize that she was discussing kennel club business, and that the bitch in question was, indeed, a dog. Still, the shock of that word coming so easily from that educated, lipsticked mouth, stayed with me.
I felt a similar kind of shock the first time I read Carolyn Kizer's poem, "Bitch." The penultimate poem in her collection Mermaids in the Basement: Poems for Women, it follows many an elegant if angry recreation of classical mythologies. Those poems — for Sappho, for Hera, for Persephone — do a profound and political job of recasting old tales in new terms, holding our western literary traditions up to the burning light of the feminist magnifying glass.
But "Bitch" is another thing altogether. There is no liberating persona, no distancing history to soften this poem's scrape and rasp. There are two simultaneous dialogues in it: one between the poet and an old lover she's run into, and another between the poet and the "bitch" inside her, who, as in the conversation, is indeed a dog. The poem begins simply:
Now, when he and I meet, after all these years,
I say to the bitch inside me, don't start growling.
He isn't a trespasser anymore.
I'm going to assume that the speaker here is Kizer herself — famously beautiful, obviously successful, and, as evidenced in the preceding poems, ever so wise. When she says to that inner bitch, "he isn't an enemy now," the reader relaxes. So far, so good.
But while the old lovers continue their small talk, a different danger arises:
At a kind word from him, a look like the old days,
The bitch changes her tone. She begins to whimper.
She wants to snuggle up to him, to cringe.
Down, girl! Keep your distance
Or I'll give you a taste of the choke chain.
The poet goes on to explain that the creature in question is "basically loyal" to her, but remembers the days of devotion to this other master, days when she survived on his "small careless kindnesses." When, at the poems' end, the old lovers part, the woman offers him the usual "regards to your wife," while the dog gags on its chain.
Coming as it did after the lengthier, more elegantly crafted poems in Mermaids in the Basement, this 34-line, single-stanza poem shocked me when I first read it. It shocks my students when I teach it now. Yes, there's that word itself, with all its electric charge. But what really delivers the blow, at least to this reader, is Kizer's blunt illumination of one of the darkest corners of the female psyche. Protecting oneself in love isn't simply a matter of steering clear of abusers, philanderers, and con artists. It's a matter of containing and controlling your own worst self — the one that wants to be praised and petted and called a good girl, who feeds on the patient patronage of men. The bitch, according to Kizer in this brilliant, brave poem, is the enemy inside. While there may be no sending her away — we are, after all, all animals — self-defense requires that we recognize and stifle her unruly, self-sabotaging ways. Rose Solari
I came to Carolyn Kizer by way of Theodore Roethke, or to put it more precisely, through my reading of Roethke's On Poetry & Craft, for which Kizer wrote an appreciative foreword. I love Roethke and loved the book, so my curiosity for Kizer's poetry was piqued.
At the time I ordered Cool, Calm, & Collected, nothing I read pleased me. I started books and stopped. I returned books half-read to the library. Speakers were trying to charm me, make me like them. I found them taxing. I became especially fond of Jack Gilbert, not because his poems are beautifully crafted in the meditative style I love but because his speaker couldn't care less whether I liked him or not. The poems struck me as honest and made no demands. They made excellent company.
As I made my way through Kizer's collected poems, the first thing I noticed was how in my face they were. They dared me not to like them, to put the book down, and, as perverse as it may sound, I liked them for that. A steady stream of anger runs through her poetry: anger at her mother, anger at the cardboard-sided suburbs, and anger at the treatment of women. It does not let up. With no lack of sarcasm, the speaker ridicules men and women of all stripes. Her speaker is not afraid to be not nice. In fact, she's not interested in being nice. She's interested in calling it as she sees it. To make her case, she does not turn to the lyricism of her teacher, Roethke, but to the rhymed and metered argument of Pope.
One of Kizer's primary subjects is the situation of women writers. In "Pro Femina," a five-part poem published over three decades, woe is not only the male writer who crosses her path—Nietzche, Kierkegaard, and Strindberg, to name just a few—but also the female "toast-and-teasedale" sonneteers, the "middle-aged virgins," and the "barbiturate-drenched Camilles." Kizer's willingness to be so unapologetic about her point of view takes nerve.
Since reading her book, I've sometimes wondered how much of Kizer's directness comes from having studied with Roethke. Gilbert was one of Kizer's classmates, as was James Wright; the class must have been extraordinary. For me, the thread that unites them is the integrity of their individual voices. They do not compromise. Kizer says in her foreword that Roethke believed his students' "eccentricities" were part of their "true voice" and that he encouraged his students to write like no one but themselves. As a teacher herself, Kizer was not interested in having her students write the perfect "workshop poem, in which everything eccentric or outrageous has been ironed out," the kind of poem you read and think, "Very nice," and never think about again. Kizer wants poems that present a challenge.
When I read Kizer, who was a major player in feminism's second-wave, I argue with her, half-agree with her, get tired of what often seems like a loud and heavy hand as is my luxury. Kizer is a poet who gets herself in the game and says exactly what she thinks. She does not sit on the side-lines, watching, casting judgment, smoothing her skirt. She does not smile politely as she comes down from the stands. She's in there, throwing the occasional elbow, ready to foul or overshoot the hoop. She's on the court because she wants to change the rules. Joelle Biele
The appreciations from Ed Skoog, Rose Solari and Joelle Biele all appeared originally in the Spring & Summer 2011 print edition of Poetry Northwest. for more about and by Carolyn Kizer, visit that page. The piece from Nance Van Winckel appears here for the first time.


text:
Wednesday, May 28, 2008
Oooh, ooooh, Opera Cake!
Daring Bakers time again! I was pretty excited when the theme for this month was announced: Opera Cake! As you might be able to tell from my photos, I didn't really have much of an idea what it should look like, so I decided to just go my own way on that. It has four, or five components:
-nutty meringue-like cake layers
-syrup to moisten the cake layers
While normally done in chocolate versions, we were asked to keep things light and spring-timey, and we were given recipes for almond cake layers, a neutral buttercream and a white chocolate mousse and glaze. I decided early on that I'd go with raspberries and pistachio, so pistachio-almond cake layers, a simple vanilla syrup, a raspberry buttercream, and a white chocolate mousse. I opted to skip the glaze - I feared it would make the cake overly sweet, and besides, I don't trust my frosting skills enough to pour a smooth glaze. (Oh, we're supposed to be... daring? Sorry.) I added some whole raspberries for a nice, fresh and fruity touch.
So. The cake layers! I used about 150 g of pistachios, and 75 g blanched almonds. I ground them together in my almond grinder (which is technically my neighbor's, I still haven't returned it...) and then proceeded with the recipe. I used round springform pans (as I didn't have a rectangular pan of a size that would work here), but... well, I had a bit of a problem. I was aiming for three cake layers, but I guess I didn't estimate well enough because one ended up much thicker than the others. I did keep it in the oven for longer, but when I went to assemble the cake, it turned out to be almost completely uncooked in the center. Oops! So, my cake only has two layers. Never mind...
Syrup. I actually didn't make this, as I had some vanilla syrup in the fridge from previous experiments.
Buttercream. I was a bit scared, because people in the forums had said that they'd had problems with this. I was in fact so scared that I turned to my own buttercream recipe, adapted from Rose Levy Berenbaum, instead. I didn't have quite enough unsalted butter at hand, so instead of the 225 g I used in that recipe last time, I only used about 185. No difference as far as I can tell, though. And I added cold-pressed raspberry extract rather than raspberry purée - and, the flavor is at least as good as when I used actual raspberries. Go figure! I did add a smidge of color as well, raspberry buttercream has to be pink, right?
On to the mousse. Where I again, didn't trust the recipe. I've tried the "fold melted chocolate into whipped cream" before, but I like my mousses to be a bit sturdier than those... So - I went for a slightly different approach: I chopped about 200 g of white chocolate finely and put in a bowl. I then heated 300 ml of cream, and poured it on top of the chocolate. I stirred it all together until the chocolate was completely melted and then I put it in the fridge overnight. Next day, on the day of cake assembly, I whipped it like you would any whipping cream, and it turned into a beautiful, thick, silky mousse. (This is a great method for making cake filling actually - it works with pretty much any chocolate, and I've used melted candy before, very yummy. But you MUST leave it to cool overnight.)
And no glaze. So for assembly, I moistened my cake layers, covered the first layer with raspberry buttercream and raspberries, placed the second layer on top, covered it with more buttercream and then frosted the sides as well. I refrigerated it until it was really firm, and then used the white chocolate mousse all over the cake. A final touch: a sprinkling of pistachios.
This cake was a huge hit! I served it for my birthday party and everyone really loved it. I personally am not a big fan of buttercream in cakes, I find it too stiff and well, too buttery. But the whole raspberries lightened things up, and kept the cake from being too sweet. And the white chocolate mousse was delicious!
You can find the recipes here, the blogroll here, and the discussion forums - open to everyone! - here. The four hosts this month was Ivonne of Creampuffs in Venice, Lis of La Mia Cucina, Fran of Apples Peaches Pumpkin Pie and Shia of Whiskful.
45 comments:
- Rosa's Yummy Yums said...
Very beautiful! Your lovely cake looks delicious!
Rosa - May 28, 2008 11:08 AM
- Deeba PAB said...
Happy belated birthday Anne...the cake looks so pretty & good enough to eat off the screen! YUMMY...I loved reading your post!
- May 28, 2008 11:19 AM
- Jessica said...
Coming from an opera house near you:
Ich sterbe hier; ich sterbe da
;) - May 28, 2008 11:20 AM
- Katie said...
ohhh it looks and sounds wonderful. The raspberry layer is so pretty and sounds delicious
- May 28, 2008 11:59 AM
Whole raspberries and then pistachios....yummy!!!
- May 28, 2008 12:06 PM
- Liz said...
I like the your variety with the pistachios and raspberries. We love raspberries - wish I had thought of that! Beautiful cake!
- May 28, 2008 12:10 PM
ROund -now that is daring! Great looking cake.
- May 28, 2008 1:17 PM
- Sarah said...
It looks SO good! I want to take a bite!
- May 28, 2008 1:34 PM
- Jeanine - The Baking Beauties said...
Beautiful cake! I bet the pistachios & raspberries went well together.
- May 28, 2008 1:40 PM
- Dagmar said...
It looks gorgeous! Unfortunately I never found any time to make the cake due to the move....
- May 28, 2008 2:19 PM
- Valerie Harrison (bellini) said...
First of all happy birthday. Great job with your Opera Cake as well..inspired ingredients. I like the fact that the raspberries would cut back on the sweetness:D
- May 28, 2008 2:44 PM
I love what you did with the mousse! Mine wasn't stiff enough.
- May 28, 2008 2:50 PM
- Renee said...
Your cake looks yummy! I love the berries!
- May 28, 2008 3:07 PM
The raspberries look delicious. Using them was a good idea to keep the sweetness down. Beautiful job!
- May 28, 2008 3:09 PM
- Bumblebutton said...
Lovely job, and so pretty! Bet it was yummy!
- May 28, 2008 4:01 PM
- Mary said...
Your cake is so pretty! And I love the flavour combo of raspberries and pistachios!
- May 28, 2008 4:09 PM
- glamah16 said...
Great flavor Ann. What a nice birthday treat.
- May 28, 2008 4:35 PM
Looks great, Anne. very pretty indeed!
- May 28, 2008 5:43 PM
- - - MayMay - - said...
OOooh indeed! This looks great!
- May 28, 2008 6:15 PM
- Vera said...
You couldn't go wrong with the raspberries! Looks lovely!
- May 28, 2008 8:16 PM
- Thistlemoon said...
It looks absolutely gorgeous! I love all the colors! Those pistachios...I could pick on right off the top!
- May 28, 2008 8:46 PM
You made a great version of an opera cake. It looks delicious!
- May 28, 2008 8:54 PM
- Helene said...
Love the way you went with yours Anne! It looks wonderful!
- May 28, 2008 8:57 PM
- Dolores said...
I'm loving the flavor combinations that people have come up with. Raspberries and pistachios... yum! Great job!
- May 28, 2008 10:52 PM
- CookiePie said...
Your cake is so pretty - and I love the raspberry-pistachio combination! The mousse is such a beautiful finish for the cake, great idea!
- May 28, 2008 11:08 PM
- Shelby said...
Happy Birthday - and what a beautiful cake! Great Job!
- May 29, 2008 12:21 AM
- maybelles mom said...
Beautiful, and i love raspberry and pistachio.
- May 29, 2008 3:20 AM
Mmm - I love the raspberry and pistachio combination! Hope you had a happy birthday!
- May 29, 2008 4:38 AM
- Elle said...
Even before I read it was for your birthday, I thought "That cake looks like a party!" with the pink and green and white. Happy Birthday a little late. Although a bit unorthodox, you cake is gorgeous.
- May 29, 2008 5:36 AM
- dailydelicious said...
Oh, I really love your cake, it's lovely, and Happy Birthday (quite late Ha Ha.
- May 29, 2008 6:06 AM
- Suzana said...
Great combo- that looks delightful! Happy belated birthday, Anne. :)
- May 29, 2008 11:50 AM
I think I have the wrong blog - Can we swap?
I will do beautiful pink cakes and you can cook turnips...
Is it a deal? - May 29, 2008 3:42 PM
- Sheltie Girl said...
Happy Birthday! Beautiful job on your Opera Cake.
Natalie @ Gluten a Go Go - May 29, 2008 4:17 PM
- Gabi said...
Love the pistachios sitting on that lovely cloud-like cake- the raspberry flavour sounds good with the combo of pistachio and almond too! :)
- May 29, 2008 8:33 PM
- Meeta K. Wolff said...
Simply gorgeous! It looks lovely. I love pistachios so I am loving this too!
- May 29, 2008 9:15 PM
- Barbara said...
Yum, raspberries and pistachio's. Great choice. Your cake looks great.
- May 29, 2008 11:47 PM
- Lisa T. said...
Wow - this looks so luscious! I love the pistachios on top.
- May 30, 2008 1:11 AM
Looks delicious!
- May 30, 2008 2:12 AM
- Shari@Whisk: a food blog said...
Raspberries and pistachios sound delicious.
Shari@Whisk: a food blog - May 30, 2008 3:16 AM
Your colours are awesome - love the icing, nice job! :)
- May 30, 2008 6:35 AM
- myriam said...
jesus, i was so stupid: didnt think about this great, great combination of pistacio & raspberries. great job!
- May 30, 2008 11:21 AM
- Chris said...
Love it! raspberry and pistachios = a match made in heaven! :)
- May 30, 2008 4:17 PM
- Unknown said...
I love the addition of the pistachios on top...wonderful!
- May 31, 2008 2:26 AM
Wow, this is really beautiful. Inspired combination!
- May 31, 2008 11:56 PM
- Brownie said...
What a combo! Your photos are making me hungry! I agree with you about buttercream, it's generally a bit, well, buttery for me. I added strawberry to mine and it added a nice light flavor.
- June 02, 2008 3:17 AM


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To carry out patch repairs our team mix EPDM rubber granules with a polyurethane resin binder. We then fill in the gap to fix the soft surface.
If it is very minor damage, we may be able to simply fix the wearing course, however we may also need to repair the base rubber.
Your wetpour playground surface can begin to reduce in size all around the outsides with time. This leads to the outer perimeter to start to reduce in size causing cracks across the play area. This may become a big tripping threat for kids and so it's important to sort the issue promptly.
If you do not resolve the problem rapidly then your issue may result in more costly methods. We've got a repairs service which is developed to help deal with these problems by filling out the spaces using your chosen EPDM rubber granules.
Creating a servicing plan set in place is vital to making sure these don't happen again. These servicing plans hugely enhance the quality of the sports facility near me.
It'll furthermore guarantee that the area is always in a greater condition enhancing its product life. We can fix these border concerns in an alternative colour rubber to develop a perimeter border effect. Our wet pour perimeter repairs are available at an affordable cost and can completely renew your area.
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best prices guaranteed with free no obligation quotes
GET IN TOUCH NOWOur team can also prevent accidents by making certain the area meets bs en 1176 standards.
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We could offer you wet pour repair kits that contains rubber along with polyurethane binder so that you can fix the problem yourself. We can advise whether a wetpour repair kit would be beneficial or not.
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With regards to artificial grass, we can carry out full replacements if the existing surface is looking tired. We can install the same colour or different colour to ensure you get exactly what you need on your surrounding area.
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We are happy to answer any questions you may have regarding rubber mulch and wet pour surfaces and offer you our expert service.


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Happy New Year Black-Eyed Pea Soup
I'm excited about 2010 and all the fun and anticipation a New Year brings. It doesn't look like I'll be holding a baby on my lap while watching the ball drop, however. This new little one is not following the path my other two took of arriving at exactly 38 weeks, but instead is teasing with lots of contractions and then nothing for a few hours.
2010 may be the year I develop a great deal of patience.
or completely lose my marbles.
the jury's still deliberating.
I'm not bringing a slow cooker dish (shocking, I know!) to our friend's house tonight for New Years At 9 because I wasn't sure if we'd have a newborn or be in the hospital. Instead, the kids and I are going to make a fabulous apple pie thanks to the totally awesome gluten free pie shells from Whole Foods.
and tomorrow? Tomorrow we'll eat Black Eyed Pea soup. This is the recipe I used last year, and I'm looking forward to making it again. I'm going to re-print it the way it appeared a year ago.
Have an absolutely wonderful 2010 full of lots of love and hugs.
CrockPot Black Eyed Pea Soup
Day 366.
because it's Leap Year.
Happy New Year's Eve! I made the most rockingest black eyed pea soup yesterday, and I take back everything I said last year about black eyed peas. They aren't gross. I just didn't know what I was doing.
And thank you also for all of the emails explaining pig anatomy. I get it now.
Although this is the end of my year-long challenge, the blog will not die, and I will not go hide in a corner in the fetal position. I promise.
Eating black eyed peas on New Years Day is good luck and is said to bring prosperity which can't be a bad thing. Ring in 2009 on the right note. Make this soup.
The Ingredients.
--1 pound dried black eyed peas
--1 pound spicy sausage (I used Aidells chicken habanero and green chile)
--6 cups chicken broth
--1 yellow onion, diced
--1 cup diced carrots
--1 cup diced celery
--4 cloves garlic, diced
--1/2 tsp Italian seasoning (not pictured. I didn't know I needed it at first. But I did.)
--1 tsp kosher salt
--1/2 tsp black pepper
--Tabasco sauce (to add at the end to taste)
The Directions.
Soak your beans overnight. Drain and pick out the undesirables (broken, discolored beans) in the morning.
Use a 5 to 6 quart crockpot. This will serve about 8 people. Dice the veggies, and dump them into your crockpot with the pre-soaked beans. Add sliced sausage. Pour in broth, and stir in Italian seasoning, salt, and pepper.
Cover and cook on low for 8 hours, or on high for about 6. Before serving, use a stick blender to smash up about 1 cup of beans. If you don't have a stick blender, scoop out 1 cup of beans, blend them in a traditional blender, and add back to the soup. Don't blend too much---just enough to get the broth thicker and creamy-looking.
Ladle into bowls, and add Tabasco sauce to taste.
The Verdict.
This tastes amazing. I am so glad that I tried cooking black eyed peas again---they are earthier than other beans and make a fantastic soup base. I've got a few different tupperwares ready to go for lunches for the next few days. My brother and sister-in-law liked it too. The kids didn't taste it---they had spaghetti for dinner.


text:
- 52
- Fonds
- 1955
The fonds consists of 28 black and white photographs taken by Codere in 1955 during her second field work expedition to B.C. 19 of the photos were taken in and around Alert Bay, while the remaining 9 were taken on Hope Island.
Sans titre


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Our correspondence team receives many gifts from sponsors for their sponsored children that can't be sent to our country offices. If this happens to your gift, we will contact you to see if you'd like the gift returned or donated to a local Colorado Springs charity, Northern Churches Care.
And to help prevent that from happening to you, which items do you think can be sent to your sponsored child through the mail?
Ok, ok. It was a trick question. None of the items can be sent through the mail. Unfortunately, we receive about 300 items like these each month. Our correspondence team spends about eight hours each week sorting out these undeliverable items.
During the Christmas season, rather than donating the items to Northern Churches Care, the correspondence team gathers all of the items that couldn't be sent and that weren't returned to sponsors and makes shoeboxes to donate to Operation Christmas Child.
Last year, they were able to donate over 200 shoeboxes.
You can find a list of items that you CAN send to your child through the mail on compassion.com.
UPDATE: November 30, 2011 – Here's an idea of the volume of items we receive that can't be sent and which sponsors choose not to have returned.


text:
If you need new, fresh marketing initiatives, then evolve your advertising to include a podcast. A podcast is a radio or video show that is streamed online or downloaded. Podcasts are most often listened to on portable media players. This medium is on the forefront of gaining better understanding of your audience and, therefore, more leads.The podcast, theoutdoorsation.co.uk showed a boost of 20% in sales of products mentioned in his podcast. This week, we're giving you three ways a podcast will help your business, and next week we'll outline exactly how to set up a podcast:
Be seen as an expert—With 2015 being the year of content marketing, businesses want to deliver any content while being perceived as an expert. Podcasts allow you to do just that—advertise your best knowledge. What are you passionate about? What do you consider yourself an expert on? Ponder those questions and then start a podcast on a particular topic.
Focused audience—Podcasts can cover any topic. You can be as broad as "Marketing 101" or as narrow as "Social Media Marketing for Print Shops." But, this ability to create narrowly-focused shows automatically attracts other businesses as leads. Podcasts have a more loyal audience, because it's seen less as advertising and more as building a client relationship through free deliverable content.
Costs little money—The beauty about using a podcast as advertising, is that it costs almost no money. All you need is your time, your knowledge and a computer. If your podcasts gain a large audience, then you may be investing in books and other resources to create more content. Otherwise, podcasts are a no-brainer.


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once again confirming that in many ways I am a lucky sonofabitch.
Now, I do have new piece finished. It looks something like this,
but as it's still drying the official photographs will have to wait.
Instead, do you fancy a riffle through the delicious pages of The Ladies' Manual of Fancy-Work, published in 1883 by A. L. Burt of New York, and edited by Miss Jenny June?
Jenny June sounds suspiciously like a pseudonym, doesn't it? Possibly a pseudonym for a thick-necked and paunchy cigar-smoking drapery salesman whose real name was Irv Magee?
One's suspicion only grows upon learning that Mr. Stephen Foster–who dreamt, famously, of Jeannie and her light-brown hair–also wrote a popular minstrel ballad with the refrain:
When the meadows were in tune
With the birds among the bowers
In the sweet summer time.
We're on to you, Irv Magee. We see you there, behind the pen, with your cigar.
Irv's taste in fancy-work, not unusually for the time, inclined steeply to the florid.
Among the curiosities are instructions for artificial macramé (which Irv calls macreme).
It's crochet.
Also, there are pages of line-drawings of whimsical Regency moppets suitable for transferring to embroidery fabric, drawn by a D-list Kate Greenaway impersonator who remains anonymous (and no wonder).
One of the vignettes has dialogue (click for a larger version), like a primordial New Yorker cartoon.
The Ladies' Manual of Fancy-Work was published in 1884. James Thurber was born in 1894. Just want to point that out.
There are advertisements, too, including several for yarn.
Nobody boasts anymore that their silk yarn has "dead lustre." Why not?
And since this manual of fancy-work is for ladies, there are also ads for ladies' things, like corsets.
My favorite, please don't miss it, is the "nursing" corset with the flip-top tit.
(Say that out loud a few times.
Flip-top tit.
Flip-top tit.
Flip-top tit.
You said it, didn't you? Out loud?
Are you at work?
I hope nobody heard you.)
Finally, the articles. Good stuff. Chinese embroidery, the history of the cashmere shawl, etcetera.
Here's one of them, in full.
Boys who Learned Needle-work
Thanks for sticking up for the boys, Irv! Have another cigar!


text:
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text:
Before winter is gone for good and cold days make way for colder drinks, I want to share with you one of my most favorite warm, comforting desserts ever. This bread pudding is crafted from fresh, buttery croissants, melt-in-your-mouth gourmet dark chocolate and pure love.
This is the kind of food that comes from the heart, y'all. So, sneak away from your salads and healthy resolutions for a moment, and DIG. THE HECK. IN.
All it took was a bunch of bakery croissants, chocolate, cream and strawberries, dreaming up something decadent and delicious, and taking the time to enjoy it. That is, before bikini season strikes and we all have to become responsible little eaters.
The soft croissant pieces soaked up the creamy spiced mixture you see above for just a few minutes before piling into a dish with a handful of chopped dark chocolate.
Drooling yet?
After baking for just about thirty minutes, this fabulously fluffy bread pudding was ready to be piled into a bowl with strawberries. With a little dusting of powdered sugar, a drizzle of homemade chocolate syrup (recipe coming soon), it was the perfect dessert. And, let's be honest, breakfast the next morning too!
Try this decadent treat for yourself sometime and you'll experience a slice of heaven too! The croissant base makes for a much lighter texture and taste than traditional bread puddings. With whipped cream, ice cream or any combination of fresh fruit, it makes for a enjoyable, easy dessert or breakfast!
Croissant and Chocolate Bread Pudding
- Nonstick cooking spray
- 3 large egg yolks
- 1 cup milk
- 1/2 cup heavy cream
- 1/2 cup granulated sugar
- 1/2 teaspoon pure vanilla extract
- 1/4 teaspoon kosher salt
- 1/4 teaspoon ground nutmeg
- 3 croissants, cut into 1-inch pieces (about 1 pound)
- 2 ounces bittersweet chocolate, cut into chunks
Optional topping
- Fresh fruit such as strawberries, raspberries, blueberries, etc.
- Heat oven to 375° F. Coat a small, shallow 2-quart baking dish with cooking spray.
- In a large bowl, whisk together the egg yolks, milk, cream, sugar, vanilla, salt, and nutmeg. Add the croissants and chocolate and mix to combine.
- Transfer the mixture to the prepared baking dish and bake until set and a knife inserted in the center comes out clean, about 30 minutes. Serve warm or at room temperature.
- Serve with chocolate syrup, ice cream or whipped cream and fresh fruit, if desired.
- Enjoy!


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Travel Agency 2705821
How Zicasso Selects the Best Travel Specialists
How we select the best travel specialists
Our commitment is that each travel specialist in Zicasso's network is among the finest travel agents and tour operators – experts whom we consider the top 10%, or perhaps even the top 1%, in the industry.
To ensure quality, we've spent thousands of hours pre-qualifying and selecting our travel specialist partners. During this process:
- We personally interview each potential new partner in detail. To determine their expertise and service levels, an initial interview typically lasts two hours.
- We require that travel specialists have traveled extensively within the countries and destinations they sell, and continue to travel there every year.
- We review examples of their customized travel itineraries in depth.
- We continuously monitor their industry references, professional reputations, client testimonials, etc.
4.77 out of 5 stars
4.77 stars based on 13 reviews


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If you thought last month's Supreme Court ruling upholding the Affordable Care Act was the final word on the legality of the health law, think again. Some conservative scholars believe they may have discovered a flaw that could send the law back to court, or at least cause some big problems for its implementation.
To understand the potential problem, first you have to understand a little about how the law is supposed to work. Starting in the year 2014, every state is scheduled to have a health exchange — a sort of online marketplace where individuals and small businesses will be able to go to shop for health insurance. For low- and middle-income individuals, there will be help — in the form of tax credits — to pay for the insurance.
My advice to Republicans is get over it. The law is the law and we're moving ahead with it. Quit trying to scare people.
States aren't required, however, to set up these exchanges. Indeed, many governors have already said they won't. The law takes that into account, and says if the states don't create an exchange, the federal government will set one up and run it instead.
But there's a problem.
"The statute doesn't authorize tax credits in federal health insurance exchanges; it authorizes them solely through state health insurance exchanges," says Michael Cannon, head of health policy at the libertarian Cato Institute and an opponent of the health law.
Cannon says when he first discovered the anomaly last year, he assumed it was a glitch. He says further research showed it might have been intentional. In a paper out this week, Cannon and Jonathan Adler, a law professor at Case Western Reserve University, claim Democrats decided that it was important for states to run the exchanges.
The faction that prevailed in writing the law, Cannon says, decided to offer the tax credits in exchanges established by the states and not offer them in exchanges established by the federal government because that would be a tremendous incentive for states to create exchanges.
Cannon says as a result of that decision, recent rules issued by the IRS allowing tax credits to be offered in exchanges run by either the states or the federal government are, in his words, "illegal."
"The clear language of the statute and the legislative history show that the IRS does not have the authority to do that," Cannon says.
But that opinion is far from universal.
"That's, unfortunately for them, wrong," says Timothy Jost, a professor at the Washington and Lee University School of Law and a strong backer of the health law.
Jost admits the law isn't written as clearly as it could have been. But he says it is pretty clear that a federally run exchange will be able to do everything a state exchange can, including provide tax credits, despite Cannon's claim that federal exchanges can't.
"This is an interesting theory, but it's completely contrary to the structure of the legislation and even the language of the legislation," he says.
And what about Cannon's assertion that Senate Democrats gave only the state exchanges the right to provide tax credits as a way to encourage the states to create them? A quick survey of Senate Democrats involved in writing the health law didn't uncover any who recall it that way.
"No, I think they're available in both," says Tom Harkin, the Iowa Democrat who chairs the Senate Health, Education, Labor and Pensions Committee.
Sen. Ron Wyden, an Oregon Democrat and senior member of the Finance Committee, remembers it the same way.
"That's correct, and I make that judgment based on everything that came up in the Senate Finance Committee and the fact that the exchanges were something that were popular on both sides of the aisle," Wyden says.
In fact, Democratic senators like Ohio's Sherrod Brown say that those who are still trying to challenge the law are just out to make trouble.
"They're just going to keep trying to find things in the law that they think, for whatever reason, won't work," Brown says. "I just think they should be ashamed of themselves."
Harkin was more blunt. "My advice to Republicans is get over it. The law is the law and we're moving ahead with it. Quit trying to scare people," he says.
But between now and Election Day, opponents of the health law are likely to continue to hammer at it any way they can.
Copyright 2021 NPR. To see more, visit https://www.npr.org.


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- 8
- Fundo
- 1919-1977 [predominant 1919-1923]
Fonds consists of four photographs, and correspondence between Evelyn Goddard and the Museum of Anthropology regarding the donation of the images. The photographs include two snapshots taken by Goddard of indigenous graves at Hagwilget, BC, a photograph showing scenes around Hazelton, BC, and a postcard featuring the image of a totem pole at Kitseguecla, BC.
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