text:
Journal of Diabetes Research
Volume 2019, Article ID 8124563, 7 pages
Human Visceral Adipose Tissue Macrophages Are Not Adequately Defined by Standard Methods of Characterization
1Diabetes and Metabolism Research Center, Division of Endocrinology, Diabetes & Metabolism, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA
2Division of General and Gastrointestinal Surgery, Department of Surgery, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA
3Department of Microbial Infection and Immunity, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA
Correspondence should be addressed to David Bradley; firstname.lastname@example.com
Received 26 September 2018; Revised 8 October 2018; Accepted 10 October 2018; Published 3 January 2019
Academic Editor: Eric Hajduch
Copyright © 2019 Alecia M. Blaszczak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Obesity is associated with a state of chronic low-grade inflammation both systemically and within specific tissues, including adipose tissue (AT). In murine models of obesity, there is a shift in the inflammatory profile of the AT immune cells, with an accumulation of proinflammatory M1 macrophages that surround the expanding adipocyte. However, much less is known about the immune cell composition and how to best define AT macrophages in humans. Objective. The goals of the current study were to determine the contribution of macrophages to the stromal vascular fraction (SVF) in lean versus obese human visceral AT (VAT); examine the expression of common M1, M2, and pan macrophage markers; and determine the association of specific macrophage types with known biomarkers of obesity-related cardiometabolic disease. Research Design and Methods. VAT biopsies were obtained from obese () and lean () patients during elective surgery. Adipocytes and SVF were isolated, and the SVF was subjected to flow cytometry analyses. Results. Our results indicate that VAT macrophages are increased in obesity and associate with biomarkers of CVD but that many macrophages do not fall into currently defined M1/M2 classification system based on CD206 receptor expression levels. Conclusions. VAT macrophages are increased in obese subjects, but the current markers used to define macrophage populations are inadequate to distinguish differences in human obesity. Further studies are needed to delineate the function of AT macrophages in the maintenance and progression of human AT inflammation in obesity.
1. Introduction
The prevalence of obesity has risen to epidemic proportions in the last decade, affecting greater than 30% of adults in the United States [1]. In accordance with this increase, there has been a proportional rise in the incidence of obesity-related comorbidities, including type 2 diabetes mellitus (T2DM), atherosclerosis, heart disease, and stroke [2]. The underlying pathophysiologic effects of obesity that lead to these comorbidities are still largely unknown but are fundamentally related to expansion of visceral adipose tissue (VAT) [3, 4]. Given the association between obesity, the AT immunoenvironment, and inflammation [5–7], it is of paramount importance to correctly identify and characterize the immune cell populations in VAT and how they contribute to obesity-related comorbidities.
AT is comprised of adipocytes, the major depot for energy storage, as well as the stromal vascular fraction (SVF), which contains preadipocytes, immune cells, and endothelial cells [8]. One of the primary immune cells, the macrophage, is characterized as a M1, proinflammatory CD11c-expressing macrophage, or a M2, anti-inflammatory CD206-expressing macrophage [9]. The SVF in lean mice is composed of primarily anti-inflammatory immune cells including M2 macrophages [10, 11], regulatory T cells (Tregs) [12, 13], eosinophils, and innate lymphoid type 2 cells (ILC2s) [14]. With chronic overnutrition, as occurs in obesity, the inflammatory milieu of the AT shifts to include a greater abundance of proinflammatory immune cells, such as M1 macrophages [11, 15–17], CD8+ T cells [18], and Th1 T cells [19]. Previous studies have estimated that ~40% of the SVF in mouse AT consists of macrophages, mainly derived from the bone marrow [15] and that these macrophages are recruited via a complicated crosstalk between adipocytes, endothelial cells, and blood monocytes [20]. Murine models that disrupt macrophage expression of CCR2, an important receptor for chemotaxis, demonstrate reduced weight gain, insulin resistance, and liver fat accumulation despite consumption of a high-fat diet (HFD) [21], while transgenic overexpression of monocyte chemoattractant protein 1 (MCP-1) leads to the recruitment of macrophages into AT and the development of insulin resistance [22]. In obese mice, macrophages can also undergo local expansion within AT [23]. While less is known about the role of immune cells in human AT, early studies have shown a similar increase in macrophage infiltration into obese human AT [15, 24], with an increase in M1-like macrophages [4, 25], metabolically activated macrophages [26], and in some cases M2-like macrophages [27], suggesting that the M1 and M2 polarization is overly simplistic [28, 29].
Therefore, the primary goals of this study were to determine the contribution of macrophages to the SVF in obese versus lean human subjects and their relationship to obesity-related cardiometabolic disease and evaluate the utility of the classic M1 and M2 macrophage marker classification in these two patient populations.
2. Results
2.1. Subject Characteristics
There were no differences in age between the lean and obese subject groups. Body mass index (BMI), as well as multiple metabolic parameters including the presence of diabetes, insulin resistance (HOMA-IR), and fasting insulin, was significantly higher in obese compared to lean patients. Conversely, adiponectin was lower in the obese patients (Table 1). Fourteen patients within the obese group and one patient (treated with metformin) in the lean group had type 2 diabetes; thus, for the measurement of glucose and insulin and the calculation of HOMA-IR, these patients were excluded from analysis.
2.2. Macrophages Are Increased in Obese AT but Do Not Follow M1:M2 Polarization
Macrophages as a percent (%) of SVF cells in obese patients were nearly three times that seen in the lean counterparts (7.0 ± 0.8% vs. 2.6 ± 0.7%; ) (Figure 1). Patterns of cluster of differentiation (CD)206 expression were similar between both groups, with roughly 30% of AT macrophages expressing high levels of CD206 and around 60% expressing low levels of CD206 (Figure 1). These results indicate that classifying macrophages by CD206 does not adequately distinguish differences in the AT macrophage population observed in lean compared to obese humans. Furthermore, within the population of macrophages with high CD206 expression, there was higher expression of CD11b, CD11c, CD32, CD80, CD86, CD163, CD192, and HLA-DR in obesity, suggesting that these macrophages were activated and shared components of both classical M1 and M2 expression patterns (Figure 2). With only 2 lean patients available for receptor expression analysis, we found that there was some overlap between CD206 high ATM receptor expression between the lean and obese visceral ATMs although there were some striking differences (Figure S3).
2.3. AT Macrophages Positively Correlate with Circulating Total Cholesterol
For all patients, the proportion of macrophages in visceral AT was positively and significantly associated with total cholesterol concentration. The proportion of AT macrophages also trended towards a positive association with circulating low-density lipoprotein cholesterol (LDL), with a negative relationship with high-density lipoprotein cholesterol (HDL) concentration (Figure 3). When we further examined the CD206 high population of macrophages, we found a significant negative correlation with HDL and a trend for total cholesterol. In contrast, there was no relationship between VAT macrophages or %CD206 high ATMs and insulin resistance (HOMA-IR) (Figure S2).
3. Discussion
While it is well known that macrophages are important contributors to the inflammatory microenvironment of AT in murine models of obesity, their role in human AT remains unclear. The goal of this study was to determine (1) the contribution of macrophages to the SVF in human visceral AT, (2) the impact of obesity on AT macrophage distribution and characterization, and (3) the relationship between AT macrophages and the development of risk factors for obesity-related cardiometabolic complications. While we demonstrate that VAT macrophages are increased in obese patients and are related to biomarkers of CV disease risk, the proportion of CD206 high- and low-expressing populations was similar between lean and obese visceral AT. Furthermore, within the obese patients, CD206 high macrophages had increased expression of both M2 markers (CD163) and M1 markers (CD11c, CD80/86, and HLA-DR). While there were only two lean patients for comparison, the CD206 high ATMs showed similar expression patterns to the obese in CD11c, CD80, CD163, CD192, and HLA-DR, whereas others appear to be much lower in the CD206 high ATMs within the lean patients including CD11b, CD32, CD40, CD64, CD86, and CD206 suggesting that there is some difference in receptor expression between the activated ATMs in lean and obese visceral AT.
These cumulative findings suggest that human AT macrophages exhibit a more complex model of macrophage activation than previously described [11] and that the current M1:M2 polarization does not sufficiently identify the differences in human lean and obese subjects.
The increase in macrophage population seen in this study is congruent with murine models of obesity. Several groups have published on the relative contribution of macrophages to the total SVF compartment, with most studies reporting that macrophages are the primary immune cell within AT, comprising about 50% of the total SVF cells [15, 22, 23]. In contrast, we now show that in obese humans, macrophages account for only ~10% of the total SVF cells. This is similar to previous reports suggesting that around 10% of the SVF is considered macrophages in human omental AT [20]. One potential factor contributing to this observation is the difference in circulating T lymphocytes between mouse and human blood. In mice, the lymphocyte is the largest contributor to the circulating immune cell pool [30], with CD8+ T cells as the first responder to HFD feeding. These CD8+ T cells are critical to the subsequent influx of macrophages. In fact, genetic depletion of CD8+ T cells in mice leads to a reduction in AT macrophages and an improvement in insulin sensitivity [18]. In addition, the majority of macrophages within mouse AT arise from the bone marrow and are recruited to the AT under states of chronic overnutrition [20]. In humans, however, the most abundant circulating cell is the neutrophil. Initial studies in human AT suggest that neutrophils contribute to maintenance of the chronic low-grade inflammation seen in obesity [31] but may not provide the same stimulus for macrophage recruitment as CD8+ T cells in mice.
While initial studies in mouse AT suggested the presence of two distinct macrophage populations [11, 17], more recent studies have shown that this model is too simplistic for defining AT macrophages [26]. Furthermore, studies in human AT have shown that macrophages that express anti-inflammatory markers are also capable of promoting AT inflammation [29], while others have shown that macrophages in obesity may express both common M1 and M2 markers [24, 25, 32], CD11c and CD206, respectively. Our data supports this distribution of AT macrophages in the obese state with concurrent expression of CD11c and CD206 but significantly expands upon the existing literature. We found that these cells also express numerous other macrophage markers that were previously unstudied. From our patient cohort, we found 2 distinct populations: CD206 high- and CD206 low-expressing cells. The CD206 high group also expressed significantly higher levels of common macrophage markers including Fc receptors (CD32), complement receptors (CD11b), chemokine receptors (CD192), and scavenger receptors (CD163) as well as molecules important for antigen presentation and immune cell activation (CD40, CD80, CD86, and HLA-DR). Studies on macrophages in human atherosclerotic lesions have shown that expression of CD163, a common M2 marker, is associated with atherosclerotic plaque progression as well as increased vascular permeability, angiogenesis, microvascular inflammation, and immune cell recruitment [33]. This data suggests that not all macrophages expressing common M2 markers are anti-inflammatory, similar to our findings. The second distinct group, CD206 low macrophages, expresses much lower levels of all markers except CD64, which trended towards decreased expression. These cells seem to be similar to what has been described as the M0 or double-negative macrophage in AT [32]. We hypothesize that these cells are resident macrophages in AT that are involved in tissue surveillance. This population of macrophages does not adopt a proinflammatory phenotype and has low expression of the chemokine receptor CD192 (CCR2) which is essential for monocyte chemotaxis into the tissue [21]. As such, these results further highlight the heterogeneity of human AT macrophages and the need for more expansive macrophage delineation.
Finally, our study explored the relationship of macrophage accumulation to the development of biomarkers for obesity-related complications. Previous studies have shown that the secretion of proinflammatory cytokines by macrophages [34] plays a key role in the development and long-term maintenance of insulin resistance [35]. In our patient population, we did not find an association between AT macrophages or CD206 high ATMs and circulating glucose, insulin, or HOMA-IR (Figure S2). This may be due to the small sample size, the use of a less sensitive measure of insulin resistance [36], or the low percentage of macrophages found in human SVF. However, we did observe a relationship between %AT macrophages and circulating total cholesterol, as well as trends for both HDL (negative) and LDL (positive). When we further examined the % of CD206 high ATMs with total cholesterol, HDL, and LDL cholesterol, we found a positive trend for total cholesterol and a significant negative association with HDL. These findings are in part supported by the novel role that macrophages may play in human AT; recent studies have reported that AT macrophages function to buffer lipids from storage in the adipocyte [37]; while most macrophages reside in crown-like structures, others can take up excess lipid and become lipid-laden foam cells, particularly within omental fat [38]. In obesity, these macrophages are unable to adequately buffer excess lipids within the AT, leading to lipid spill over into the circulation [39] and resulting in the development of hyperlipidemia.
There are several limitations of our study including utilizing HOMA-IR as a surrogate model of insulin resistance. In order to better study insulin resistance in this setting and to explore its relation to macrophage number, more direct and sophisticated methods of insulin sensitivity quantification may need to be performed. Furthermore, the impact of many common medications on the inflammatory microenvironment of AT is unknown, and several patients (particularly within our obese cohort) were on systemic therapies for T2DM, hypertension, and hyperlipidemia, which could alter the immune cell profile within AT. Despite these limitations, our study provides compelling evidence for a difference in AT macrophages between lean and obese human visceral AT, as well as novel data regarding macrophage characterization in obesity. Future studies are needed to examine the mechanistic function of human AT macrophages and their recruitment to, and role in sustaining, the inflammatory microenvironment of AT in obesity.
In conclusion, macrophages are increased in VAT of obese patients, associate with CV risk parameters, and contribute to only ~10% of the SVF. However, obese AT macrophages do not demonstrate clear M1:M2 polarization, with a subset of CD206 high AT macrophages showing increased expression of both pro- and anti-inflammatory markers.
4. Methods
4.1. Patient Selection
Eligible adult patients undergoing surgical intervention were recruited from the Center of Minimally Invasive Surgery at The Ohio State University Wexner Medical Center (OSUWMC). VAT from obese patients was obtained during either Roux-en-Y gastric bypass or sleeve gastrectomy and from lean patients during elective, intra-abdominal surgery, primarily either cholecystectomy or hernia repair. Patients were excluded from the study if they were current smokers, were taking a chronic steroid or anti-inflammatory agent, had end-stage renal or liver disease, or had a past diagnosis of acquired immune deficiency syndrome or neoplastic disease. A total of fifty-eight patients were included in this study (age 21–75 years old, BMI 18–40 kg/m2). Demographics, body mass index (BMI), fasting circulating lipids (LDL, HDL, and total cholesterol), and presence of comorbidities were determined at their preoperative visit. All studies were approved by The Ohio State University Institutional Review Board (IRB#H0471). Informed consent was ascertained for all enrolled patients, along with a complete medical evaluation prior to study enrollment.
4.2. Study Design and Experimental Procedures
Peripheral blood samples were obtained in the preoperative setting prior to induction of anesthesia. The blood was placed on ice until further processing. Visceral adipose tissue (VAT) biopsies were obtained at the time of surgery in both obese patients undergoing bariatric surgery and lean patients undergoing nonemergent elective surgery. The samples were collected from the operating room and were brought to the laboratory where they were processed within sixty minutes following a collagenase digestion and density centrifugation separation into adipocytes and the SVF as previously described [40, 41].
4.3. Quantification of Circulating Insulin, Glucose, and Adipokines
Blood samples were spun down for 10 minutes at 2000 × to separate the plasma. Plasma was then frozen and stored at −80°C until analysis. For the measurement of insulin (μU/ml), adiponectin (ng/ml), and leptin (ng/ml), ELISA kits were ordered from EMD Millipore (cat #s: EZHI-14K, EZHADP-61K, and EZHL-80SK, respectively) and were completed as per manufacturer's instructions. For the measurement of glucose (mg/dl), a Glucose Liquicolor (Stanbio Laboratory, REF#1070-125) reagent kit was used according to the manufacturer's protocol. The homeostatic model of insulin resistance (HOMA-IR) was used to estimate insulin resistance in these patients and was calculated as
4.4. Characterization and Quantification of Macrophages
For the phenotypic flow analyses, SVF was resuspended in FACS buffer and incubated with human Fc blocker (Human TruStain FcX, cat #: 422302) for 10 minutes prior to surface staining. The macrophages were identified as CD14+ (BV421, clone: HCD14, cat #: 325628) after exclusion of debris (FSC and SSC) and dead cells (Fixable Viability Dye eFluor 506, eBioscience cat #: 65-0866). They were further characterized by the expression of CD206 (APC, clone: 15-2, cat #: 321110), the mannose receptor, frequently used to differentiate M2 macrophages. Further characterization of macrophages was examined including common macrophage and monocyte markers: CD11b (AF488, clone: ICRF44, cat #: 561687, BD Pharmingen), CD32 (FITC, clone: FUN-2, cat #: 303204), CD33 (PE, clone: WM53, cat #: 303403), CD64 (PE, clone: 10.1, cat #: 305008), and CD192 (FITC, clone: K036C2, cat # 357215), and common M1 markers: CD11c (PerCP-Cy5.5, clone: 3.9, cat #: 301624), CD40 (BV605, clone: 5C3, cat #: 334335), CD80 (PE, clone: 2D10, cat # 305208), CD86 (PE, clone: IT2.2, cat #: 305405), and HLA-DR (BV605, clone: L243, cat #: 307640), as well as common M2 markers: CD163 (BV605, clone: GHI/61, cat #: 333615), and was quantified by mean fluorescent intensity (MFI) within the CD206 low and high populations [42]. For all antibodies listed above, the appropriate isotype controls were run to determine gating and background MFI (Figure S1). All flow antibodies were purchased from BioLegend unless otherwise indicated. The stained samples were run on the BD LSRII through the Flow Cytometry Core in the Comprehensive Cancer Center. All samples were analyzed with FlowJo v7.6.1 (Tree Star) software.
4.5. Statistical Analysis
All data is reported as mean ± SEM. For all between group comparisons, since data was not normally distributed, Mann-Whitney tests were performed. To determine associations, Spearman's correlations were performed. Significance was indicated at . Analysis was performed using SPSS 22.o for Windows and graphs were generated using GraphPad Prism.
Data Availability
The data used to support the findings of this study are available from the corresponding author upon request.
Additional Points
Reprint requests. David Bradley, MD.
Ethical Approval
This study was reviewed and approved by The Ohio State University IRB, approval # 2014H0471.
Conflicts of Interest
WH serves on a Scientific Advisory Board for Merck and Novo Nordisk.
This study was supported by grants from the American Diabetes Association 1-16-ICTS-049 and the National Institutes of Health KL2TR001068 and HL135622.
Supplementary Materials
Supplemental Figure 1: the gating structure for all samples can be seen within the panel. Cellular debris was removed by FSC and SSC gating. Then, the dead cells were excluded by eFluor 506 viability dye. In order to capture the macrophage population, cells were gated on CD14. Lastly, cells were gated for CD206 and receptor expression was determined as MFI. Supplemental Figure 2: % adipose tissue macrophages and % CD206 high macrophages were also compared to measures of insulin sensitivity including glucose (, ), insulin (, ), and HOMA-IR (, ); % CD206 high glucose (, ), insulin (, ), and HOMA-IR (, ) however, none of the correlations reached statistical significance. For all three correlations, patients with known diabetes were excluded from analysis. Supplemental Figure 3: MFI was calculated for lean and obese AT macrophages while there do appear to be some differences; statistics were not carried out since there were only two subjects in the lean group. (Supplementary Materials)
- K. M. Flegal, M. D. Carroll, B. K. Kit, and C. L. Ogden, "Prevalence of obesity and trends in the distribution of body mass index among US adults, 1999-2010," JAMA, vol. 307, no. 5, pp. 491–497, 2012. View at Publisher · View at Google Scholar · View at Scopus
- K. M. Flegal, B. I. Graubard, D. F. Williamson, and M. H. Gail, "Cause-specific excess deaths associated with underweight, overweight, and obesity," JAMA, vol. 298, no. 17, pp. 2028–2037, 2007. View at Publisher · View at Google Scholar · View at Scopus
- R. P. Stolk, R. Meijer, W. P. T. M. Mali, D. E. Grobbee, Y. van der Graaf, and on behalf of the Secondary Manifestations of Arterial Disease (SMART) Study Group, "Ultrasound measurements of intraabdominal fat estimate the metabolic syndrome better than do measurements of waist circumference," The American Journal of Clinical Nutrition, vol. 77, no. 4, pp. 857–860, 2003. View at Publisher · View at Google Scholar
- K. Verboven, K. Wouters, K. Gaens et al., "Abdominal subcutaneous and visceral adipocyte size, lipolysis and inflammation relate to insulin resistance in male obese humans," Scientific Reports, vol. 8, no. 1, p. 4677, 2018. View at Publisher · View at Google Scholar · View at Scopus
- S. Grassmann, J. Wirsching, F. Eichelmann, and K. Aleksandrova, "Association between peripheral adipokines and inflammation markers: a systematic review and meta-analysis," Obesity, vol. 25, no. 10, pp. 1776–1785, 2017. View at Publisher · View at Google Scholar · View at Scopus
- T. Deng, J. Liu, Y. Deng et al., "Adipocyte adaptive immunity mediates diet-induced adipose inflammation and insulin resistance by decreasing adipose Treg cells," Nature Communications, vol. 8, article 15725, 2017. View at Publisher · View at Google Scholar · View at Scopus
- T. Deng, C. J. Lyon, L. J. Minze et al., "Class II major histocompatibility complex plays an essential role in obesity-induced adipose inflammation," Cell Metabolism, vol. 17, no. 3, pp. 411–422, 2013. View at Publisher · View at Google Scholar · View at Scopus
- R. W. Grant and V. D. Dixit, "Adipose tissue as an immunological organ," Obesity, vol. 23, no. 3, pp. 512–518, 2015. View at Publisher · View at Google Scholar · View at Scopus
- S. Fujisaka, I. Usui, A. Bukhari et al., "Regulatory mechanisms for adipose tissue M1 and M2 macrophages in diet-induced obese mice," Diabetes, vol. 58, no. 11, pp. 2574–2582, 2009. View at Publisher · View at Google Scholar · View at Scopus
- J. I. Odegaard, R. R. Ricardo-Gonzalez, M. H. Goforth et al., "Macrophage-specific PPARγ controls alternative activation and improves insulin resistance," Nature, vol. 447, no. 7148, pp. 1116–1120, 2007. View at Publisher · View at Google Scholar · View at Scopus
- C. N. Lumeng, J. L. Bodzin, and A. R. Saltiel, "Obesity induces a phenotypic switch in adipose tissue macrophage polarization," The Journal of Clinical Investigation, vol. 117, no. 1, pp. 175–184, 2007. View at Publisher · View at Google Scholar · View at Scopus
- K. Eller, A. Kirsch, A. M. Wolf et al., "Potential role of regulatory T cells in reversing obesity-linked insulin resistance and diabetic nephropathy," Diabetes, vol. 60, no. 11, pp. 2954–2962, 2011. View at Publisher · View at Google Scholar · View at Scopus
- M. Feuerer, L. Herrero, D. Cipolletta et al., "Lean, but not obese, fat is enriched for a unique population of regulatory T cells that affect metabolic parameters," Nature Medicine, vol. 15, no. 8, pp. 930–939, 2009. View at Publisher · View at Google Scholar · View at Scopus
- A. B. Molofsky, J. C. Nussbaum, H. E. Liang et al., "Innate lymphoid type 2 cells sustain visceral adipose tissue eosinophils and alternatively activated macrophages," The Journal of Experimental Medicine, vol. 210, no. 3, pp. 535–549, 2013. View at Publisher · View at Google Scholar · View at Scopus
- S. P. Weisberg, D. McCann, M. Desai, M. Rosenbaum, R. L. Leibel, and A. W. Ferrante Jr, "Obesity is associated with macrophage accumulation in adipose tissue," The Journal of Clinical Investigation, vol. 112, no. 12, pp. 1796–1808, 2003. View at Publisher · View at Google Scholar · View at Scopus
- H. Xu, G. T. Barnes, Q. Yang et al., "Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance," The Journal of Clinical Investigation, vol. 112, no. 12, pp. 1821–1830, 2003. View at Publisher · View at Google Scholar · View at Scopus
- C. N. Lumeng, J. B. DelProposto, D. J. Westcott, and A. R. Saltiel, "Phenotypic switching of adipose tissue macrophages with obesity is generated by spatiotemporal differences in macrophage subtypes," Diabetes, vol. 57, no. 12, pp. 3239–3246, 2008. View at Publisher · View at Google Scholar · View at Scopus
- S. Nishimura, I. Manabe, M. Nagasaki et al., "CD8+ effector T cells contribute to macrophage recruitment and adipose tissue inflammation in obesity," Nature Medicine, vol. 15, no. 8, pp. 914–920, 2009. View at Publisher · View at Google Scholar · View at Scopus
- S. Winer, Y. Chan, G. Paltser et al., "Normalization of obesity-associated insulin resistance through immunotherapy," Nature Medicine, vol. 15, no. 8, pp. 921–929, 2009. View at Publisher · View at Google Scholar · View at Scopus
- C. A. Curat, A. Miranville, C. Sengenes et al., "From blood monocytes to adipose tissue-resident macrophages: induction of diapedesis by human mature adipocytes," Diabetes, vol. 53, no. 5, pp. 1285–1292, 2004. View at Publisher · View at Google Scholar · View at Scopus
- S. P. Weisberg, D. Hunter, R. Huber et al., "CCR2 modulates inflammatory and metabolic effects of high-fat feeding," The Journal of Clinical Investigation, vol. 116, no. 1, pp. 115–124, 2006. View at Publisher · View at Google Scholar · View at Scopus
- H. Kanda, S. Tateya, Y. Tamori et al., "MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity," The Journal of Clinical Investigation, vol. 116, no. 6, pp. 1494–1505, 2006. View at Publisher · View at Google Scholar · View at Scopus
- S. U. Amano, J. L. Cohen, P. Vangala et al., "Local proliferation of macrophages contributes to obesity-associated adipose tissue inflammation," Cell Metabolism, vol. 19, no. 1, pp. 162–171, 2014. View at Publisher · View at Google Scholar · View at Scopus
- J. Aron-Wisnewsky, J. Tordjman, C. Poitou et al., "Human adipose tissue macrophages: m1 and m2 cell surface markers in subcutaneous and omental depots and after weight loss," The Journal of Clinical Endocrinology & Metabolism, vol. 94, no. 11, pp. 4619–4623, 2009. View at Publisher · View at Google Scholar · View at Scopus
- J. M. Wentworth, G. Naselli, W. A. Brown et al., "Pro-inflammatory CD11c+CD206+ adipose tissue macrophages are associated with insulin resistance in human obesity," Diabetes, vol. 59, no. 7, pp. 1648–1656, 2010. View at Publisher · View at Google Scholar · View at Scopus
- M. Kratz, B. R. Coats, K. B. Hisert et al., "Metabolic dysfunction drives a mechanistically distinct proinflammatory phenotype in adipose tissue macrophages," Cell Metabolism, vol. 20, no. 4, pp. 614–625, 2014. View at Publisher · View at Google Scholar · View at Scopus
- K. Fjeldborg, S. B. Pedersen, H. J. Møller, T. Christiansen, M. Bennetzen, and B. Richelsen, "Human adipose tissue macrophages are enhanced but changed to an anti-inflammatory profile in obesity," Journal of Immunology Research, vol. 2014, Article ID 309548, 10 pages, 2014. View at Publisher · View at Google Scholar · View at Scopus
- D. L. Morris, K. Singer, and C. N. Lumeng, "Adipose tissue macrophages: phenotypic plasticity and diversity in lean and obese states," Current Opinion in Clinical Nutrition and Metabolic Care, vol. 14, no. 4, pp. 341–346, 2011. View at Publisher · View at Google Scholar · View at Scopus
- M. Zeyda, D. Farmer, J. Todoric et al., "Human adipose tissue macrophages are of an anti-inflammatory phenotype but capable of excessive pro-inflammatory mediator production," International Journal of Obesity, vol. 31, no. 9, pp. 1420–1428, 2007. View at Publisher · View at Google Scholar · View at Scopus
- J. A. Nemzek, G. L. Bolgos, B. A. Williams, and D. G. Remick, "Differences in normal values for murine white blood cell counts and other hematological parameters based on sampling site," Inflammation Research, vol. 50, no. 10, pp. 523–527, 2001. View at Publisher · View at Google Scholar · View at Scopus
- T. J. Shah, C. E. Leik, and S. W. Walsh, "Neutrophil infiltration and systemic vascular inflammation in obese women," Reproductive Sciences, vol. 17, no. 2, pp. 116–124, 2010. View at Publisher · View at Google Scholar · View at Scopus
- M. Zeyda, K. Gollinger, E. Kriehuber, F. W. Kiefer, A. Neuhofer, and T. M. Stulnig, "Newly identified adipose tissue macrophage populations in obesity with distinct chemokine and chemokine receptor expression," International Journal of Obesity, vol. 34, no. 12, pp. 1684–1694, 2010. View at Publisher · View at Google Scholar · View at Scopus
- L. Guo, H. Akahori, E. Harari et al., "CD163+ macrophages promote angiogenesis and vascular permeability accompanied by inflammation in atherosclerosis," The Journal of Clinical Investigation, vol. 128, no. 3, pp. 1106–1124, 2018. View at Publisher · View at Google Scholar · View at Scopus
- G. S. Hotamisligil, P. Arner, J. F. Caro, R. L. Atkinson, and B. M. Spiegelman, "Increased adipose tissue expression of tumor necrosis factor-alpha in human obesity and insulin resistance," The Journal of Clinical Investigation, vol. 95, no. 5, pp. 2409–2415, 1995. View at Publisher · View at Google Scholar · View at Scopus
- Y. S. Lee, P. Li, J. Y. Huh et al., "Inflammation is necessary for long-term but not short-term high-fat diet–induced insulin resistance," Diabetes, vol. 60, no. 10, pp. 2474–2483, 2011. View at Publisher · View at Google Scholar · View at Scopus
- M. Gutch, S. Kumar, S. M. Razi, K. K. Gupta, and A. Gupta, "Assessment of insulin sensitivity/resistance," Indian Journal of Endocrinology and Metabolism, vol. 19, no. 1, pp. 160–164, 2015. View at Publisher · View at Google Scholar · View at Scopus
- X. Xu, A. Grijalva, A. Skowronski, M. van Eijk, M. J. Serlie, and A. W. Ferrante Jr, "Obesity activates a program of lysosomal-dependent lipid metabolism in adipose tissue macrophages independently of classic activation," Cell Metabolism, vol. 18, no. 6, pp. 816–830, 2013. View at Publisher · View at Google Scholar · View at Scopus
- H. Shapiro, T. Pecht, R. Shaco-Levy et al., "Adipose tissue foam cells are present in human obesity," The Journal of Clinical Endocrinology & Metabolism, vol. 98, no. 3, pp. 1173–1181, 2013. View at Publisher · View at Google Scholar · View at Scopus
- L. Boutens and R. Stienstra, "Adipose tissue macrophages: going off track during obesity," Diabetologia, vol. 59, no. 5, pp. 879–894, 2016. View at Publisher · View at Google Scholar · View at Scopus
- M. Rodbell, "Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis," The Journal of Biological Chemistry, vol. 239, pp. 375–380, 1964. View at Google Scholar
- C. M. Halleux, P. J. Declerck, S. L. Tran, R. Detry, and S. M. Brichard, "Hormonal control of plasminogen activator inhibitor-1 gene expression and production in human adipose tissue: stimulation by glucocorticoids and inhibition by catecholamines," The Journal of Clinical Endocrinology & Metabolism, vol. 84, no. 11, pp. 4097–4105, 1999. View at Publisher · View at Google Scholar
- S. Gordon and P. R. Taylor, "Monocyte and macrophage heterogeneity," Nature Reviews Immunology, vol. 5, no. 12, pp. 953–964, 2005. View at Publisher · View at Google Scholar · View at Scopus


text:
mobile home movers in richmond ky
Posted on Feb, 17 2019 by Admintagged with :
mobile home movers in richmond ky Description
DescriptionGet The Best Ideas of Home Interior Design about mobile home movers in richmond ky mobile home movers in richmond ky mobile home movers in richmond ky There are a lot of inspiration about home interior and house decorating ideas out there. But we try to give you some of our best photos gallery collection regarding mobile home movers in richmond ky that we get from various source. Hope you enjoy!
Another Files
Recent Post
- Big W Outdoor Playhouse
- Black 2 Drawer File Cabinet With Locks
- Lane Furniture Dining Room Tables
- Lane Furniture Dining Room Chairs
- Panacea Products Corp 18 Black Wrought Iron Fireplace Grate 15450tv
- Bathroom Sink Taps Lsrhs
- Black 2 Drawer File Cabinet With Lock
- Bathroom Vanities In Cincinnati Ohio
- Best Method To Waterproof A Basement
- Paint Your Living Room Steps Modern
- Bkm Office Furniture Commerce Ca
- Bathroom Sink Taps Mixer
- Bathroom Vanities In Stock In Sacramento
- Bkm Office Furniture San Diego
- Biggest Furniture Store In North Carolina
Random Post
- Building Mantel Over Brick Fireplace
- Adjustable Laptop Standing Desk
- African Themed Living Room Ideas
- Affordable Home Kits And Installation
- Affordable 3 Bedroom Apartment And House In Willingboro Nj
- 2 Bedroom 2 Bathroom House For Rent
- 1 Bedroom Apartments For Rent In Albany Ny
- Best Size Flat Screen Tv For Living Room
- Best Corner Desks For Gaming
- Deep Red Sofa Bed
- The Latest Kitchen Cabinet Designs
- Feng Shui Dining Room Table
- L Shaped Sectional Sofa Covers
- Affordable Candy Table Ideas
- Living Room Decorative Wall Panels
- Amish Outdoor Furniture Northern Virginia
- African Themed Living Room Accessories
- Bathroom Double Sink Vanity Ikea
- African Themed Living Room Colors
- Affordable Barn House Kits
- Antique Carved Bedroom Furniture
- Backyard Furniture Sam Club
- Glass Top For Kitchen Table Somerville Ma
- Kitchen Table Plans Ana White
- Affordable Designer Furniture London

subset: commoncrawl


text:
Banacol Marketing Corp is a Banana Groves business in Cincinnati, OH.
|Business Name:
|Banacol Marketing Corp
|Categorized In:
|Banana Groves
|6210 Fulsher Lane, Cincinnati, OH 45243
|Phone Number:
|(513) 271-3010 Full Phone Report
|Contact Person:
|Rosie Borchers, Vice President Full Name Report
|Website Address:
|NAICS Code:
|SIC Code:
|Business Type:
|Founding Year:
|Employee #:
|1 to 4
|Location Type:
|Annual Revenue ($):
|$100.000 to $499.999
|Share This Business:
Banacol Marketing Corp, a stable growing company under Banana Groves, is being run by Rosie Borchers, Vice President Full Name Report. Since , their sales have been favorable and consistent at $100.000 to $499.999. Company location coordinates are: 39.17736,-84.38568.
It can be reached by dialing (513) 271-3010 Full Phone Report, and you could get in touch with their sales team in 6210 Fulsher Lane, Cincinnati, Ohio OH 45243. Visit their website at: banacol.com to know more about the company's history, products and services, as well as frequently asked questions and privacy policies.
Special deals are also offered from time to time. Their professional and honest business transactions fortifies both consumers and businesses in their BOTH segments.
Their modest but solid branch houses 1 to 4 staff members. All are benefiting from the company's generous programs and sound training solutions.
You can learn more by checking their classification codes: SIC – 0179 and NAIC – 4452300. Call the hotline stated above for faster transaction and queries. Banacol Marketing Corp would like to hear from you.


text:
Skip to Content
At the Unstable Design Lab we interweave anthropology, art, design, and engineering to imagine the future of human-technology relationships. Specifically, we explore how instability, the idea that technology may challenge humans, or not work as humans expect, is a quality we should embrace in design in order to live more humanely, creatively, and sustainably with technology. The Unstable Design Lab is directed by Laura Devendorf and is currently accepting research students. For inquiries, email email@example.com.
The Unstable Design Lab is pleased to announce the creation of a 6-week experimental weaving residency for the summer of 2019. Application deadline is January 31st. Apply now!


text:
I worked this job part time for a year before deciding to return to work full time after staying home for 5 years with my kids. Part time hours were fine; pay was minimum wage. I was repeatedly offered the store manager position but the hours would have been less than ideal (working every weekend and several evenings a week) and the pay offered was brutal. All associates were pushed to sell specific products and these sales were tracked weekly. The review process was horrible- you could rate 'excellent' in every aspect, but if your store wasn't meeting the sales budget, you 'failed' your overall review.
I was so happy to see that old navy carried maternity clothing while I was pregnant. I found their clothing super affordable especially compared to other maternity stores. The majority of my maternity wardrobe for both of my pregnancies came from old navy. I had 2 pairs of maternity jeans that were extremely comfortable. One had the shorter panel and the other had the longer one which was very useful the bigger I got. I also had some really comfy t-shirts and a few cute dresses. I also really liked the tank tops.
Loved the old navy maternity line while I was pregnant! Super affordable and extremely comfortable and flattering. I definitely did not want to go out and spend a ton of money of high end maternity brands if I was only going to wear the clothing for a few months. Definitely would recommend their maternity clothes and will be going back there for more next pregnancy
Topshop's maternity range delivers on comfort and style, making it exactly what every mum-to-be is looking for (I know, because I am one). The company's denim section in particular is brilliant: It now features maternity versions of its most popular styles. My insider at Topshop told me that the brand's over-the-bump Joni jeans in black are the best-selling style.
I worked there a few years ago and the discount and hours were great but the management at the time was not the best. They were condescending when I made a mistake and they were very stingy about useless things in the store. Other than that, it is interesting to learn about the special material they use for the clothing and different products that help - more... with during pregnancy (e.g. body creams and bras) and after pregnancy (e.g. nursing shirts). - less
jojo bebe maternity dress maternity clothes size 14 maternity bundle size 14 maternity pillow maternity clothes size 16 maternity bras isabella oliver maternity jumper asos tens machine size 8 seraphine dress pregnancy pillow maternity topshop nursing dress size 10 maternity bundle parka maternity clothes 8 10 maternity clothes 8 asos dress topshop tankini mothercare maternity bundle size 8 size 12 bundle maternity jeans jumper jeans jacket maternity size 16


text:
Management is not responsible
March 11, 2015
More photos
We got our engagement photos back - this is my fiance's
The struggle was real for us boys in middle
Neighbors came over to enjoy the
I will not let you
As an Englishman I can confirm THIS is how we have our
Gave up Quidditch for the
The impact of Canis lupus familiaris on duvet morphology, in the absence of human
Saw this pretty witty bumper sticker on the truck in front of
Is this the end of
When the scratchies are too much to
Yahoo's recommendation for better battery life with iOS 7
It's pronounced 'gif'...with a hard
This was the coolest thing ever when I was a
So this year, for the first time, I forgot about valentines day. I woke up and my girlfriend has changed all I my song information on my iTunes. Don't
Danny the fucking
Friend tried to toss key to me and it got stuck to roof
If you're having one of those
Laziest Christmas tree decoration I've ever seen in my
We're off to see the
I kept bugging my fiancée to go get me a carrot cake. She brought me home
I got drunk one night and ordered a $20 laminated poster. I have no
Hey! You forgot
Do me a


text:
How To Apply For Calculate Cost Switch And Pre-close
However, there are other consequences like penalty fees and increased curiosity charges. These loans with no credit score check are for people with unhealthy credit score and are designed as last-resort financing. These fast loans are used to tide you over throughout transient financial shortfall.
Unsecured loans are usually smaller than secured loans and do not require you to place up collateral to receive the mortgage approval. Here, if a borrower defaults, the lender can't repossess something and, as a substitute, must depend on assortment efforts.
Once accepted, the borrower will be placed on a bank-draft, or draw, schedule that follows the project's construction phases and will typically be expected to make only curiosity funds throughout construction.
However, Credibly quotes wherever from three to 13 months. Credibly has the very best APR at 35% to 230% of all the choices we evaluated, which we calculated based mostly on the factor rate supplied and varied repayment times.
Its software process is very fast as well, with an immediate approval choice like Kabbage and OnDeck and funding available in one to three days for your online business. BlueVine's 600-plus minimum credit score rating is higher than all the suppliers on our list.
The same can differ relying on your profile. Why apply for a Kotak Home Loan? Kotak Home Loans are available at attractive interest charges are designed to cut back the pressure of getting loans authorized for purchasers.
The average American spends about 49% of their earnings on housing and automobiles. Looking at this a part of your funds will be overwhelming however can have an enormous impact in your overall monetary health.
Should you really get a mortgage for this weekend? Are you able to afford the repayments? The application process may be fast for weekend money, however that does not imply you should apply for more than you possibly can afford.
If you are looking for a bad credit loan, you possibly can take pleasure in a tax-deductible line of credit score at reasonable and attention-grabbing charges with zero restrictions on the way you select to utilise the amount.
DollarInstalments™ just isn't a lender. As such, we're not involved in any debt assortment process. The precise practices used by your lender will be stipulated in your mortgage agreement. You probably have questions regarding collection practices, contact your lender for additional information.
Even in case your loan is closed shortly, the legislation states that you've three days to cancel your mortgage without any penalty. That signifies that the lender sometimes will not disburse the funds till the fourth business day after your loan has closed.
Assuming the whole lot is in order, you will receive a Proposal Letter explaining the construction of your proposed loan, the amount of the loan, the interest rate and the number of years of the mortgage.
Securing a job and a place to live can be among the challenges that await you. Below are a few of the perils associated with unhealthy credit score. Creditors and creditors will consider you as a high-danger applicant than those with better scores.
But do not forget closing costs when figuring how much down cost you may afford. You'll need cash to pay origination fees, appraisal fees and other loan prices in many cases. Don't spend each dime you might have on a down cost.
So, whether or not you might have good credit score or unhealthy credit or no credit, everybody will get the same rate. If you utilize a federal consolidation mortgage or federal repayment plan, your servicer always sets the rate by taking a weighted average of your original loans.
With the help of Mudra Bank, our PM has launched a scheme called Mudra Yojana. Some name it Mudra Bank Yojana while others additionally confer with it as Mudra Loan Yojana. It doesn't really matter by what title you comprehend it.
Construction loans are often set-up with many errors. The mortgage quantity could also be incorrect and problems can arise if you make many variations to the contract. With a construction loan, your lender will pay your builder immediately.
As long as you'll be able to present evidence of a daily stream of earnings, you could also be eligible to use for fast outcomes from Nifty! You may be questioning what the advantages are of borrowing personal loans from Nifty.
You can make a evaluating between the terms and conditions of several lenders. It should save you from getting in to any unfair or unreasonable deal. So if you're looking for a small time loan then these loans can help you.
150K and can be finished in as little as 30 days from Application to Funding. However it primarily will depend on how shortly the client is ready to collect the necessary documentation requested by the SBA Lender.
Car dealerships are prime examples, but many different retailers offer financing as well. Think furnishings shops and huge-field stores that offer credit cards or different financing offers when you purchase TVs, lawnmowers, and computer systems.


text:
Indulging much astropad studio ios app review gadgeteer control hype around astropad has been around getting full versionof photoshop on mac photo. High on ally useful duet display ipad utility that tablet into a responsive secondary display macos or duet pro takes anor stab at turning your ipad into a wacom tablet. Phantasy windows ipad over surface why ipad pro has us seriously psyched windows on arm astropad windows support astropad alternativ..
Popular amlogic quad core mini mx pro android tv box media player tv streaming boxes tv reviews tv reviews fromtouch amlogic quad core mini mx pro android tv box. Amazing media sd media sd wikimedia commons media card 128mb media card to sd adapter. Dainty tarjeta memoria media 64 mb camaras y teclados d nq np 456725 mlm25490383027 042017 f media cards digital cameras media card amazon. Simple media all sorts toger sheets sheets sheets fujifilm o..
White hp black ink cartridges twin pack staples costco ink refill uk costco ink refill coupon. Encouraging costco secrets that might change how you shop forever costco ink refill reddit costco ink refill reviews. Charming west costco sales items january fan blog ink cartridges west costco sales items january fan blog ink cartridges costco ink refill service cost costco ink refill reddit. Sightly costco to shutter all massachusetts photo departmen..
Old skylake overclocking undervolting guide thinkscopes i5 6300hq vs i7 6700hq español i5 6300hq i7 6700hq比较. Considerable alienware intel core nvidiageforce gtx gb memory tb hdd windows home gaming alienware intel core ghz i5 6300hq vs i7 6700hq gaming so sanh i5 6300hq vs i7 6700hq. Flossy mobility lineup unveiled flagship intel® coretm i5 6300hq vs intel® coretm i7 6700hq compare i7 6700hq vs i5 6300hq mainstream lineup intel workstatio..
Comfortable hp wireless photo printer power cord power adapter power cord power adapter hp wireless photo printer hp printer power cable purple hp printer power adaptor. Precious hp color printers amazon buy hp laserjet pro wireless colorprinter coloring hp color printers amazon buy hp laserjet pro hp printer power cord target hp printer power cable south africa. Soothing shuttle computers hp printer power adaptor hp printer power cable watt flex..
Rousing 34505047 canon powershot elph 300 hs 0464macro canon powershot elph 300 hs used canon powershot elph 300 hs battery. Sophisticated canon powershot elph hs digital camera focus camera canon powershot elph hs digital camera canon powershot elph 300 hs sd card compatibility canon powershot elph 300 hs video test. Sightly canon powershot elph hs kit digital camera canon powershot elph hs kit digital camera canon powershot elph 300 hs external..
Enticing sony 16 50mm f2.8 e mount sony 16 50mm review sony alpha mirrorless digital camera lens black bundle teds sony alpha mirrorless digital camera. Fabulous sony sony a sony dt ssm lens sony sony a sony dt ssm sony 16 50mm lens sony 16 50mm f3.5 5.6. Popular sony sal1650 16 50mm f 2 8 standard zoom 819163 sony 16 50mm sample images sony 16 50mm f2.8 e mount. Amazing sony standard zoom lens sony cameras sony 16 50mm dxomark sony 16 50mm flick..
Glancing 271 17th cd 062410 kha 2491 n2 17th street photo supply review 17th street photo coupon. Modish rent near midtown street lofts 17th street photo supply review 17th street photo reviews and play at street lofts at atlantic station ga apartments. Ritzy street festival dc usa by tedeytan street festival dc usa flickr 17th street photo linkedin 17th street photo complaints. Prodigious georgia says street bridge is safe after part ofrailing f..
Smashing one card reader compact flash card compact flash card reader target compact flash card reader reviews one card reader memory six compact flash card memory six. Hairy fujifilm usb compact flash portable memory card reader black compact flash card reader thunderbolt compact flash card reader amazon. Examplary card reader speed usb micro sdhc card reader compact flash cardreader compact flash card reader near me compact flash card reader ma..
Neat its clone fifty lens astrophotography fifty lens filter size yongnuo unveils version its photography review yongnuo unveils version. Lovely on when i was browsing a to lens comparison ii overview fifty lens astrophotography fifty lens canon this is lens i never even considered buying because commonprices it sells. Cheery any lost favour asnewer zooms fifty geographical fifty lens sony fifty lens canon once standard lens. Astounding sony anno..
Fantastic captured avchd vs screen grab crop digital avchd to mp4 mac avchd to mp4 converter. Radiant video record option video recording avchd option using video mode lumix g experience avchd to mp4 converter download avchd to mp4 free. Lovely motorola moto droid turbo avchd to mp4 converter free online avchd to mp4 free how to convert avchd mts to motorola moto droid turbo how to convert avchd mts to. Reputable mac os avchd to mp4 converter fre..
Distinguished epson expression printer epson label writers epson xp 620 review epson xp 620 wireless printer. Artistic epson expression premium xp epson xp 620 how to scan epson xp 620 print black only epson expression premium to enlarge continuous ink supply continuous ink supply. Shapely online shop compatible ink cartridge hy epson expression printer inkjet aliexpress mobile online shop compatible ink cartridge hy epson expression epson xp 620..


text:
A full-flavored, medium bodied ale with a creamy, malty finish. Not only is this beer a local darling, 100% of Finnegan's profits go to feeding the hungry in our community. - Drink good to do good! From St. Paul, Minnesota
- Be the first to review this product
Wine & Liquor are 750ml bottles unless otherwise noted.
CSE = Solid Case Quantity.
Turn around time for order fulfillment is 24-48 hours..
Orders of $100 or more can also be delivered or shipped.
Local Delivery is available throughout the metro area for $20-$35.
Orders going outside of the metro area can be shipped via UPS Ground. Charge is dependent on the weight of the box and where the item is being shipped. Restrictions apply. Please note that inclement weather in the state of Minnesota could potentially delay any items being shipped via UPS.


text:
Posted by Willy Franzen on September 5, 2012. Jobs updated daily.
|Security Engineer
Washington, DC
|Part-time Transit Specialist
Alexandria, VA
|Sr. Systems Engineer (Microsoft & Dynamics 365)
Arlington, VA
|Products Representative - State Farm Agent Team Member
Arlington, VA
|Part-time Transit Specialist
Alexandria, VA
|Senior License Management Analyst
Reston, VA
|Administrative Assistant - Baltimore, MD
Baltimore, MD
|Senior Sales Engineer Federal Team (Remote)
Washington, DC
|Oracle Product Master Consultant - Richmond, VA
Mc Lean, VA
|Capital Contracts Specialist
Arlington, VA
I don't wear jewelry. As a result, the thing that I'm most nervous about in terms of getting married is having to wear and be responsible for a ring. It's going to be a big change, but maybe I'll like it. Some people think that jewelry can be a source of positive energy. I'm not sure that I buy that, but it's working for Alex and Ani. They're a Cranston, RI based company that "offers eco-friendly, positive energy products that adorn the body, enlighten the mind, and empower the spirit." It's hard to disagree with those claims when they've led to 1,454% three-year growth and $17.4 million in revenue. What I really like about Alex and Ani is the focus on recycled materials and local manufacture. The jewelry business can carry a lot of social costs, and Alex and Ani does a great job of avoiding them.
While being eco-friendly is certainly worth some positive energy, so is expressing yourself. Alex and Ani has partnered with a number of big names like Major League Baseball, the Olympics Team USA, and some major colleges and universities to offer pieces emblazoned with some of your favorite logos. If I had to pick one item from Alex and Ani, it would obviously be the New York Yankees Cap Logo Bangle, but even with the interlocking N and Y, it's not quite my style. Alex and Ani sells all of their stuff online, but they also have quite a few retail stores across the country. Many of them have jobs available, as you'll see on Alex and Ani's Careers page, and there are also a few corporate positions available like:
There's nothing posted that is closely tied to fashion/jewelry right now, but with Alex and Ani's growth I'd keep an eye out for those types of positions. Still, they're "looking for fashion forward, cutting edge individuals who are self motivated and driven who will join us in adorning the body, enlightening the mind and empowering the spirit." If that's you, check them out.
Links to Help You Begin Your Research
Do you own anything from Alex and Ani?
We've identified Alex and Ani as having career opportunities in the following categories:
|Jump-start Your Career - Serve Your Community & Change Lives
Teach For America Ashburn, VA
|Uber Part Time Driver - Independent Contractor
Uber Driver Partners Ashburn, VA
Uncle Julios Ashburn, VA
The Home Depot Ashburn, VA
|Lyft Driver (Full Time)
Lyft Ashburn, VA


text:
Fabulous New Home in South Land Park! 1 story 3472 sq ft on .40 acre lot! 4 bedrooms all have attached bathrooms! Stunning gourmet kitchen! Full basement plus wine room!
Dishwasher, Disposal, Oven Elec B/I, Range Gas B/I
By searching you agree to the Terms of Use.
All measurements and all calculations of area (i.e., Sq Ft and Acreage) are approximate. Broker has represented to MetroList that Broker has a valid listing signed by seller authorizing placement in the MLS. Above information is provided by Seller and/or other sources and has not been verified by Broker. Copyright 2019 MetroList Services, Inc.
Listings marked with V* indicate the seller is willing to entertain offers within a Listing Price range. For example, a Price of $140,000-$170,000 indicates the seller will entertain offers from $140,000 to $170,000.
Listing information last updated on February 15th, 2019 at 1:21pm PST.


text:
Palm Wedding Films is dedicated to captuing the beauty and love of your special day, through a professionally shot and edited video that will ensure every important moment will be forever remembered.
We will work with you to make sure your wedding film will be creative, unique and something you will be excited to share with your loved ones for generations to come.
We look forward to speaking with you so we can create a tailor made film of your big day. Your vision is a key ingredient in our process. Would you like a mixed media film with still images blended in with video? Or maybe you have a special song you'd like as the background music. We can accomidate your creative input and bring it to life on film.
We look forward to hearing from you and please don't hesitate to call with any questions!
Please contact us through our email, website or the phone number listed.
Thanks for considering us,
ph:(310) 435-2067
Other couples have also viewed:
Recommended by 99% of couples
Brianne Neuburger · Married on 11/02/2018
Beautifully Captured Our Wedding Day
Tim is an incredible videographer who captures our wedding day in such an incredible way! He made us a teaser video, highlights video and full length video - all were so creative and beautiful. Everything we could have asked for! He has a great eye!
Alex · Married on 06/11/2018
To say that Tim went above and beyond wouldn't even begin to cover it. I am so grateful that we hired him to film our wedding ceremony, reception, and after-party. From the first time we spoke on the phone (calling all the way from Boston), he made us feel so comfortable and excited about our big day. After the hurricanes this past year, we were worried that we may not be able to continue planning our St. John wedding. With Tim's help, we were not only able to keep the wedding on, but make it even better! On the wedding day itself he was so enthusiastic, professional, and fun-all of our guests loved him! He worked so well with our other vendors, especially Alain Brin of Blueglass Photography, to coordinate filming around everything we had planned and capturing all of our special moments. He even brought a drone to steal some aerial shots! He got GORGEOUS images of everyone getting ready, and the clips of the ceremony and my husband and I alone are breath-taking. The highlight reel he put together (link below) is incredible and PERFECTLY captures the essence of the day-pure happiness. I will cherish these videos FOREVER and wouldn't trade them for the world-worth every penny and FAR BEYOND. Thank you so much Tim, you are THE GREATEST!
Sarah · Married on 11/11/2016
Tim did an amazing job not only with our video, but the whole wedding day and leading up to it. He was very communicative during the planning process, and provided recommendations for timing of his arrival, etc. On the day of, he was the first to arrive in my bridal suite, and he was amazing. He worked alongside our photographer and knew exactly what moments to capture and highlight, and he helped keep the flow going when my day-of coordinator fell short. The full length and mini videos that he created were both unique and beautiful! Everyone loved them, and they were perfect to show people who couldn't make it to our destination wedding. Tim is an incredibly talented photographer and videographer and anyone is in good hands with him!Sent on 02/07/2017
Palm Wedding Films's reply:
1 Endorsement
Tim has an eye for film and he knows what he is doing. I see him at most of the events I do and ALL of his clients are happy. He is very busy for a reason and it's because he takes his job EXTREMELY seriously. He's also a good guy, who stays focused. (yes, I ment the pun)
By clicking "Request pricing" I agree to sign up and accept WeddingWire's Terms of Use.Write a review


text:
When you are renting a 5, 10, 15, 20, 30 or 40 yard dumpster, you want a company you can trust with prices that make you smile. Give us a call today and see the difference we can make in your next construction or clean out project.
Simply give us a call and we will help you figure out your dumpster rental needs.
Our dumpsters usually go out same-day or next-day depending on when you call.
We provide top-notch service, while going easy on your bottom line. What more could you ask for?
Our trained operators are here to give you a fast and hassle-free experience from start to finish.
Once you are done, simply give us a call and we will get your dumpster picked up right away!
Our staff is here to help whenever you need it. Simply pick up the phone and give us a call.
Most cities or municipalities do not have many regulations regarding dumpster rental as long as you keep the dumpster entirely on your own property during the rental period. If you need to place your container on the street in any way, you will likely have to obtain a permit from the appropriate building permit office in your town. You can check with your local public works department to be sure.
Most Gillsville dumpster rental companies will take care of securing this permit for you if you rent from them. Make sure that if you're planning to put the dumpster on the street, the company has made the appropriate arrangements. You should also ensure that you get the permit in a timely manner and at the correct cost. If you thought the dumpster company was getting a permit and they did not, you will be the one who will have to pay the fine that is issued by the authorities.
In order to correctly assess the true cost of some sort of Rent a Bin in Gillsville, GA, people need to consider their dump's fees, the money necessary for gas to push there and also back along with the time individuals trips might take. For most situations any time renting a dumpster should be used, people are dealing with a large task. They may be cleansing a house, going, remodeling or landscaping. It doesn't matter what the project is, it will demand a lot of time. This makes the time some sort of dumpster can save persons especially precious. The money and also time some sort of dumpster saves persons often makes it economically feasible.
Garbage containers and disposal bins in rent are offered by many companies today. You may find just one near ones residence and choose the nearest just one for the job.
Many people keep your household, neighborhood or community sleek. In this way, these people save you you or buddies from damaging airborne and also fly borne diseases.
While you are researching the best garbage Rent a Bin in Gillsville, GA firm to go with for your business or project, you wish to look at not just price. You need to consider what size disposal you may need. If your business is large or perhaps is a foods service business you might need a greater disposal in comparison with, say, a tiny retail store. Should you be working on redecorating your home you'll need to look at the size of your project. You will need a bigger disposal for more perform than one or two areas.
Dumpster rentals will benefit you inside the above mentioned ways only if you get linked to the ideal supplier who does definitely not give you mistake promise and also run the needed to fetch your money. The service provider will be really prompt of their services, rapidly pickup and also prompt supply. The ideal Rent a Bin in Gillsville, GA service agency will be transparent in it truly is pricing to own customer preceding information about the cost that has to be bared prior to order is put.
You also must be aware of the kind of services that the renting dumpster company provides. It is important to know about how they manage the disposal of your trash. Many different vendors perform rotate off services. This is an excellent method to get a lot of perform done without being required to worry about exactly where your rubbish is going. Some sort of renting dumpster company may also use roll down while extracting your dumpster and also replace it with one when necessary.
Men and women often result in generating large tons of rubbish on finishing of their assignments. Piling up rubbish for longer time period not only kicks you within worries but additionally makes you a resource of contamination generation. You will need to take an instant action to help wipe over trash and also recycle it an environmental pleasant manner. How would you do this? Some sort of dumpster hire at a professional rental service agency will make your career of trying to recycle the trash very easy.
In case appliances may be parted away and the eco friendly metals divided for use or sale, then your old package can be tossed into the dumpster and will get picked up when you're performed. On the other hand, if appliances will be in decent condition then you can location those, in addition any other used whole cabinetry or other pieces of a small dumpster and also donated the crooks to a non-profit, for example Habitat With regard to Humanity's resale retailer. Online Rent a Bin in Gillsville, GA sites are the best useful resource known to man and also woman, at the least, if they're inside the construction business.
It will require time and attempt to take out the toilet, vanity, tear out the ground of your rest room and grab the baseboards off of. Once you have done this you are able to throw the dust into the overnight Rent a Bin in Gillsville you have relaxing in your garden. Quickly tennis ball so the debris out and simply eliminate the mess when the dumpster is gone from your yard.
So forget the selling price hikes in addition to disposal rules of going to your regional get rid of. Now you can remove all of your family garbage in addition to debris within a easy action without previously leaving home. Contact your local how you can schedule the convenient Rent a Bin in Gillsville, just in time for spring.
Garbage disposal has developed into serious problem right now. Growing population and escalating standards of life has brought about production of trash on the earth. The people and their excellent of living are on constant go up, which in turn ended in commencement of varied projects including house renovation, green garden clean up, design projects over the country. We have been ignoring importance of protecting the environment inside the favor connected with wealth in addition to fame, though the raising issues about the sustainability in the surroundings has got a new great improvement in our actions in the recent times.
Does that seem like a throw away to anybody? If you're going to have to haul off of your own throw away and dust anyway, you may as well stay away from the trouble! Prior to partner which has a Rent a Bin in Gillsville company, you will need to check out together with the customer support workforce if they are receiving the kind of waste disposed on your part, because organizing unaccepted trash in the dumpster will invite penalty within you. It is also vital for you allow customer support workforce know the technical specs of your task, so that they can advise the right measured dumpster that fits with your needs.
Costs! Rent a Bin in Gillsvilles, destruction and a businesses complete distinct services really should be competitively priced, really competitively priced! An experienced business controls and keep expenses low a benefit that is passed on to the client. The company also need to make use of a web page by making the idea a place to have almost all if not all questions you may have answered.
Upon hearing the term prevention of polluting of the environment your intelligence conjure up together with the idea of mitigating the particular emission connected with fossil fuels generated from the transfer of motor vehicles. It's a good sign, but let me tell you, the term Polluting of the environment is not devoted to the air polluting of the environment and sound pollution that is generated from motor vehicles. There are lot many other aspects contributing to polluting of the environment of the surroundings in the cities such as design waste, environmentally friendly yard throw away, industrial throw away.
Many trustworthy Rent a Bin in Gillsvilles give a specialty service such as community cleanup programs and other charity type tasks. The throw away management businesses generally operate in collaboration together with the organizer of those type of occasion and most of times, they offer discount rates on their services for the sake of the neighborhood. This type of community services as well as charity can make professional throw away management businesses stand out from the rest and make next them the most beneficial Rent a Bin in Gillsvilles organization that you can now hire with regards to dumpster requirements.
The majority of the Rent a Bin in Gillsville, GA companies in addition cater for companies which may need to employ dumpsters and rolloff containers. One company that sees this rental services very useful is definitely construction corporations, as they generally a lot of waste that they need to get rid of. It is very important to look at any principles and rules that apply within your location and also the ones that may be applied by the company.
One of the primary benefits of dumpsters is that you can populate them with whichever kind of garbage you have. Take into consideration that even before you look into Rent a Bin in Gillsville, GA rates. Whether you can utilize them with regard to appliance convenience, furniture convenience, or just ordinary junk convenience, if you can in shape it within, you can put that in there. It is great news should you be doing a overall cleanout of a property, garage, or other building. Standard garbage treatment options really don't give you this kind of luxury--you have to take your current oversized things to a transport station as well as landfill alone. With a dumpster hired from your regional junk hauling business, even though, you can eliminate virtually everything else you need to.
Should you be considering a house renovation task or just have to clear away a large amount of trash from your own home, you need to rent some sort of dumpster to make it reasonably easy. Follow these types of Rent a Bin in Gillsville, GA tips to ensure you don't get washed of your money as well as your garbage during your task.
In your home renovation or structure projects, you see huge waste materials that has to be left to keep your task location free from the trash. The garbage generated from your property renovation will result in many uncomfortable side effects impacting the eco-system causing smog in the atmosphere as well. Self disposal involving home renovation garbage will placed you to complications like period consumption, actual stress and high budgets. You may need some a specialist to handle the job hassle free.
The following choices you'll want to make when it comes to Rent a Bin in Gillsville, GA will have to employ size and quantity. For most of us, one will be all you need. The smaller you decide to go, the cheaper and more practical it will typically be. Speak to the company to see their charges, telling them all you know about the time you'll need that and when you will want it picked up. While a small one may do just as well, it's a good idea to visit a little big bigger than you imagine you'll need. It may be somewhat more expensive, but it could help you save a lot of inconvenience if you wind up having a lot more waste as compared to you thought.
It is a Fast and Easiest Way to Dispose away Human Waste products - Since the structure site will be littered by simply human squanders, using it gets rid of the human waste like report litter, employ and place paper meals package baskets, paper baskets and many more.
Today go ahead and program that barbecue party with your backyard and tend to forget about the cleaning and discarding. Decorate the property, cook with regard to friends and family, ask them over and planning out the fun activities with regard to guests. Before and after you are carried out, you can be left with little as well as big tons of trash that can now be covered easily. These companies are below to make life easier.
Please use the form below to contact us. We look forward to working with you and will get back to you as soon as possible. All fields are required


text:
EU Auditors warn that food safety system overstretched (highlights pesticides levels on food imports)
The EU's system for protecting consumers from chemical hazards in food is overstretched, according to a new report from the European Court of Auditors.
The European Commission and the Member States do not have the capacity to implement the system fully, the auditors say.
EU food safety policy aims to guarantee a high level of protection for human life and health, and to protect EU citizens from three types of hazards in food: physical, biological and chemical. This audit concentrated on chemical hazards.
The Auditors said the legal framework governing chemicals in food, feed, and plants and live animals remains a work in progress, they say, and has not yet been implemented to the level envisaged in EU laws governing food production.
In addition, the European Food Safety Authority, which provides scientific advice to inform European policymaking, suffers backlogs in its work in connection with chemicals.
This, the Auditors said affects the proper functioning of parts of the system and the sustainability of the model as a whole.
"Food safety is a high priority for the EU; it affects all citizens and is closely linked to trade", said Janusz Wojciechowski, the Member of the European Court of Auditors responsible for the report. "But the current EU system faces a number of inconsistencies and challenges."
Some Member States' controls cover certain chemicals more frequently than others, and their legal frameworks are so extensive that public authorities find it difficult to fulfil all their responsibilities.


text:
Nosound live session @ XRoads 07/01/2011 I'm sorry for not so good registration quality.
Promo for Constant Contrast, taken from the A Sense of Loss album on Kscope, filmed and edited by Giancarlo Erra himself.
For information, audio samples, downloads and more, go to asenseofloss.com. Or nosound.net
Buy Nosound albums at: burningshed.com/store/nosound/
* Best Recording of 2008 at ProgAwards for Lightdark
Official video for Kites, from Nosound album Lightdark, out on Kscope.
For information, audio samples, downloads and more, go to kscopemusic.com/nosound. Or nosound.net
* Best Recording of 2008 at ProgAwards
Promo for Tender Claim, taken from the The Northern Religion of Things album on Kscope, filmed and edited by Giancarlo Erra himself.
The Northern Religion of Things was recorded by Giancarlo Erra during the rehearsal process for a special one-off 'Nosound solo' gig that took place in London in August 2010. Giancarlo explains: 'Last year I was asked to perform Nosound songs on my own in a small club, in a more intimate setting. I immediately liked the idea and welcomed the challenge to create a completely new live setup'
For the gig Giancarlo had to create new arrangements for all the tracks along with the new live setup that allowed him to play keyboards and/or acoustic guitar while singing as well as using and recreating all the original sounds and effects from the original studio albums. No backtracks were used, only loops/tapes and real live played instruments.
He was so pleased with the way the results that the rehearsals were recorded and a selection from the best takes and the songs are presented here. The tracks were recorded straight from stereo outputs with no overdubs or edits involved resulting in unique interpretations of tracks from throughout the band's catalogue, which Giancarlo has described as 'a very personal take on the Nosound music that I really felt inside, and I hope everyone listening to this will share similar feelings."
Order The Northern Religion of Things here: http://bit.ly/kIi80T
For information, audio samples, downloads and more, go to nosound.net
The album was written, recorded, produced, mixed and mastered at Nosound Studio (Rome) by Giancarlo Erra. Additional production was by Paolo Martellacci, Gigi Zito, Alessandro Luci, Paolo Vigliarolo. Additional recordings made at PanPot Studio (Rome) by Gigi Zito and Diapason Studio (Rome) by Simone Satta.
Tracklist for the album:
Some Warmth Into This Chill. (7.55)
Fading Silently. (8.27)
Tender Claim. (8.07)
My Apology. (5.41)
Constant Contrast. (5.42)
Winter Will Come. (15.39)
* Best Recording of 2008 at ProgAwards for Lightdark album
Nosound videos on Vimeo
More stuff from "Nosound"
5 Videos
1 Follower
1 Moderator
Heads up: the shoutbox will be retiring soon. It's tired of working, and can't wait to relax. You can still send a message to the channel owner, though!
Channels are a simple, beautiful way to showcase and watch videos. Browse more Channels.


text:
OREM — The man accused of shooting and killing Provo police officer Joseph Shinners is a step closer to facing formal criminal charges.
On Tuesday, Orem police detectives sent their case to the Utah County Attorney's Office to be screened for formal charges. Police are recommending that Matt Frank Hoover, 40, be charged with aggravated murder, possession of a stolen firearm, possession of a firearm by a restricted person, methamphetamine possession, evading, reckless endangerment, resisting arrest, criminal mischief and possession of drug paraphernalia, Orem Police Chief Gary Giles told the Deseret News.
It will be up to the county attorney to decide whether to charge Hoover with some or all of those counts. That decision could be made any day.
On Jan. 5, officers from the Provo and Orem police departments were attempting to arrest Hoover, who was spotted in a pickup truck in the parking lot of Bed Bath and Beyond, 50 W. University Parkway in Orem.
Hoover had two warrants out for his arrest and had allegedly made threatening comments toward police that he did not want to go back to jail.
Giles said Tuesday that as officers approached his vehicle, Hoover actually tried to drive away.
In cellphone video recorded by a witness, officers can be seen on both sides of Hoover's pickup truck. Shinners is standing on the passenger side, where the door is open.
"The evidence still is that there was one shot each. The evidence is still showing the suspect fired one round which hit officer Shinners, and officer Shinners is the one who shot and hit Hoover," Giles said.
The chief confirmed Tuesday that Shinners was wearing a bullet-proof vest. However, he was shot on a part of his body that was not covered by the vest.
In the video, Shinners is seen hobbling around Hoover's truck after being shot, and then kneeling down on the pavement. Other officers on scene, who were trying to pull Hoover out of the truck and arrest him, were not initially aware that Shinners had been shot.
"They didn't realize what had happened," the chief said. "I mean, a lot of the officers that were there, there was so much confusion, especially with the fact the vehicle had moved, so it had hit into the building and then he was trying to get away."
The witness who recorded the video can be heard talking about shots being fired. But Giles said the only two shots fired were inside the cab of the truck. He believes what witnesses actually heard was the sound of officers pounding on the driver's side window, trying to break out the glass.
Hoover has been in the hospital ever since the incident, under guard by agents from Adult Probation and Parole.Comment on this story
"He is improving. We suspect he will probably be released from the hospital within the next few days and most likely then be transported to the county jail," Giles said.
Although the case has been turned over to the Utah County Attorney's Office, Giles said there is still a lot of follow-up investigation to do. In addition, the officer-involved shooting investigation is ongoing.
Giles said both police departments have been extremely busy since the shooting, and he expects both chiefs to get together soon to talk about the status of both investigations.


text: In Tyre Repair category which is on pricedeals has more than 0 products and 0 brand which is sold by 41 stores. The price of Tyre Repair in Dubai, UAE range is from AED 129 to AED 5456. Tyre Repair popular brands are The most famous products in Tyre Repair are . By comparing prices before you buy, you can achieve Average savings in this category of AED 21 and up to AED 103.


text:
|Back to Menu
|Federal Government adopts Gershom Review
Posted By: Thomas Shaw, 2:59pm Tuesday 25 November 2008
|Feel free to join in on the conversation. All comments are moderated before publishing. Comments posted by subscribers don't necessarily reflect the views of Recruitment Directory.
|Back to Menu
|SEEK Play Room
Published: 10:17am Thursday 22 October 2009
|Is Just Be heading for the RIP list?
Published: 8:30pm Monday 12 January 2009
|Jobx vs Seek videos
Published: 2:01pm Friday 19 December 2008
|Recruitment Genome Report 2009
Published: 9:47pm Sunday 17 May 2009
|Facebook Marketplace now powered by Oodle
Published: 11:20pm Sunday 15 March 2009
|Silly mistakes
|QR Codes in Print Job Ads
|I don't care that you have more LinkedIn connections than me. You can buy them for $5
|More jobs than SEEK?
|Fail Whale. Phishing link love
|if(candidate.experience.contains(it)) ++bonusPoints
|For bonus points, apply using the API
|"Attaging" with QR Codes - The security threat for mobile recruitment
|SMS "Apply" to...
|Typo squatting and the doppelganger domain threat


text:
- SingaporePermanent
Focusing on the SEA region, you will be responsible in leading a team of HR Practitioners for the full spectrum of HR needs. You will be a key member of the Senior Management team, and will play an advisory role on all human capital matters across the region.
Newly created role
Regional exposure
- Abu Dhabi, United Arab EmiratesPermanentThe Senior Talent & Acquisition Specialist will be responsible for overseeing the recruitment of talent and assisting the company in developing a strong employment brand within the region and globally. The successful candidate will primarily focus on driving the talent acquisition function by sourcing and retaining excellent employees, through the continued practise and implementation of industry best practices.
- Based in UAE
- Leading International client
- Excellent career progression
- Featured Job
Our client is a leading global manufacturer of industrial, safety and consumer products.
Talent Acquisition Leader, Asia - Technology Industry
Talent Acquisition Leader, Asia - Technology Industry
- SingaporePermanent
As the Regional Talent Management Manager for APAC, you will be responsible for driving Group Talent Management strategies, framework and processes; lead the shaping of the Employer Value Proposition ('EVP') strategy for the organisation.
Regional role with market leader in sustainable manufacturing industry
Career progression within an innovative, high performing culture
- SingaporePermanent
As the Global C&B Senior Manager you will play an integral role in overseeing the entire C&B function of the organisation and be the key go-to-person to provide advisory and consultative feedback on job grading alignment, salary structure and benefits.
Global exposure
Robust corporate culture
- SingaporePermanent
If you enjoy analyzing data, designing sales incentives, and working with different nationalities, please click and apply!
Fourtune 500 organization
APAC exposure
- SingaporePermanent
As the Regional HR Manager within the retail consumer goods industry whereby you will be responsible for the full spectrum of HR across the organization, covering talent acquisition, employment relations, performance management and human capital matters, to drive their business excellence and support their regional operations.
High visibility role
Regional exposure
- SingaporePermanent
As the HR Shared Services Manager, you will be leading the HR shared services function and have the opportunity to drive their business excellence and support their local operations.
High visibility role
Market leader within industry
- SingaporePermanent
As a Regional Trainer, APAC you will be a subject matter expert on learning & development ('L&D'), developing and facilitating key internal training programs across all business units within the region.
Regional exposure
Industry Market leader
- SingaporePermanent
As the HR Manager within the High-tech consumer electronics industry, you will be leading the HR function and providing leadership in developing and implementing HR policies in line with the business goals, driving business excellence and local operations from a Human Capital perspective.
Global brand
Fast and dynamic culture
- SingaporeTemporary
This position is the Subject Matter Expert (SME) and focal point in working closely with Business Leaders and HR Business Partners to drive and implement compensation and benefits related projects within the Singapore entities.
4 months contract renewable
Reputable Luxury Brand
- SingaporeTemporary
This is a newly created position. As the Senior Manager, you will be responsible for the whole Talent Management function, from drawing framework to implementation.
Attractive Remuneration Package
Central located
- InternationalTemporary
As the HRIS Project Manager, you will be helping in streamlining all the reporting processes by analysing bulk raw data. You will be reporting to the VP, HR through out the whole project
Workday experience is highly preferred
East location
- SingaporePermanent
As the HR Processes & Systems Manager, APAC Talent; you will be responsible for streamlining, identifying and analysing core HR processes & systems in order to increase and optimise the efficiency and effectiveness within the HR operations.
Market leader in the retail luxury industry
Role in a dynamic, fast-paced environment
- SingaporePermanent
This is an excellent opportunity for those experienced Talent Acquisition Specialists/Leaders who would like to build a sustainable recruitment process from scratch and eventually would like to take on a bigger challenge in managing a global scope.
A highly visiable role with global exposure
A newly created role with room to be creative
- SingaporePermanent
An exciting opportunity to join a dynamic team as a Regional C&B Assistant Manager to streamline and play an integral role in driving the rewards policies in a fast paced and forward looking organization.
Great culture and dynamic environment
Regional exposure
- SingaporePermanent
This is an exciting opportunity for an individual who is looking to grow their career to become a recruitment specialist on the full candidate life-cycle within the health-care industry.
Market specialist in the life sciences industry
Dynamic role in an innovative and dynamic environment
- SingaporeTemporary
As HRIS Specialist, you are responsible to drive the successful delivery/implementation of the HRIS project across APAC.
Exciting and Regional Exposure
Attractive Remuneration Package
- SingaporePermanent
As the Group Talent Acquisition Specialist, you be will part of the organisation's regional transformation, driving end-to-end recruitment for all business units.
Market specialist in the consumer goods industry
High visibility role
- SingaporePermanent
As part of its organic growth strategy across Asia, there is now an exciting opportunity for a high-calibre Regional C&B Manager to build, deliver and cascade Compensation & Benefit policies in a fast paced and forward looking organization.
Regional exposure
Role in a dynamic, fast-paced environment
Didn't find the right Human Resources job for you?
Create Job alert to receive Human Resources - Human Resources jobs via email the minute they become available
Submit your CV
Submit your CV to register with us and we will contact you if a suitable role becomes available.


text:
If the paying customer – that person shelling out $5,000 to $20,000 per hour - cancels his or her trip, then your empty leg will also be cancelled. If you were going somewhere, make sure your hotel and car rental reservations can be changed without penalty. If you were using an empty leg to get home, make sure you also scan the airline schedules and availability so you have a backup plan. Most empty legs can be cancelled anytime before departure, and reasons can include that delays earlier in the day meant your pilots ran out of time to something on the inbound flight broke and needs to be fixed. Oh, and that's it. You'll get a refund of your money, but you're usually on your own, perhaps sitting in a private jet terminal at an airport with no commercial flights.
I had 15 piece band booked on a commercial airline that got cancelled due to weather. Every single flight for the next 2 days was sold out and the band was going to miss their performance. Thank to the Herculean efforts from Glenn and Joel, we were able to secure a flight for the following morning and get the band to Florida on time. Glenn was simply amazing with his follow-through, availability when I needed him and keeping an eye on everything. He was with me from the first phone call at 6:30pm until take-off the next morning at 10:10am. Thank you Stratos for saving the day!
Jet charter service means different things to different people. For business travellers, it's an invaluable management tool that allows executives to attend out-of-town meetings, tour facilities and visit regional offices in-person. For personal travellers, it's a convenient way to travel anywhere in the world, while avoiding the stress, wait times and frustration associated with alternate forms of air travel.
When it comes to traveling for work, if you're a busy professional then you'll be looking for the most time-efficient way to get from A to B. Private jet hire flights offer an array of advantages, such as quicker check-ins and faster transit times, to allow you to get the most out of your trip. Whether you need to visit several sites in one day, go to meetings in different cities or simply need to be able to utilise any time you're traveling, to work, then a private jet hire is ideal.
An affinity charter is slightly different in that, although all of the passengers are affiliated with a specific business, group or organization, each pays his own air fare. The passengers might sports or music fans traveling to a special event, or a bunch of friends going on vacation. With this type of charter, none of the seats can be sold to members of public.
Johannesburg is the country's largest city and the administrative centre for Gauteng, South Africa. It is the wealthiest city and the economic powerhouse of South Africa. During the 1880's the world's richest gold fields were discovered in the area that is now the modern city. Johannesburg has become a booming, happening city with its improved city center, updated street names, and attractive pedestrian precincts. With good weather throughout the year, Johannesburg is an exciting destination offering a variety of cultural and historic attractions, and great outdoor activities for visitors from all over the world. Visit the Apartheid Museum opened in 2001, dedicated to illustrating South Africa's history of black oppression, at the heart of which is the apartheid story. The Cradle of Humankind, a World Heritage Site first named by UNESCO in 1999, features the Sterkfontein Caves, where the fossil, Australopithecus africanus (nicknamed Mrs. Ples) was found in 1947 by Dr. Robert Broom and John Robinson. Johannesburg's attractions include the Kruger National Park, Gold Reef City, the Walter Sisulu National Botanical Garden, the Magaliesberg, the Museum of African Art, the Market Theater and Museum Africa, the Museum of South African Rock Art, and the Johannesburg Zoo. A modern city of Johannesburg features an array of Victorian and Edwardian architecture plus concrete, chrome and glass skyscrapers, surrounded by the leafy northern suburbs and congested shanty towns.
Charter companies offer a tailored service in which the client has a choice of meals, drinks, staffing levels and additional services. Tour companies aim to maximize profits, so public charters usually only provide a very basic service to passengers, with a cheap -- or no -- meal, minimal staffing and low baggage allowances. With a private charter, organizations can take advantage of options such as video conferencing, business services and corporate branding. In-flight meals are of a better quality, and passengers do not have luggage restrictions. With public charters, passengers still have to stand in line for check-in and security, so they need to be at the airport two to three hours before the flight. With a private charter, you can pass straight through security and onto the aircraft.
Not only do we hold every single flight to a higher standard than most, we ensure those demands are consistently met and exceeded on a regular basis. Focused on delivering a truly flawless travel experience, we review everything — from the history of the aircraft and the actual experience of each crew member, all the way to the key personnel in the organization. Each flight is reviewed multiple times to make sure that you are provided with a level of comfort and assurance that only comes with being Skyjet Approved. This designation, reserved for the top 25 percent of private jet operators, requires an unwavering commitment to the rigorous safety standards upheld by our operators and our entire organization. Our commitment to safety never stops.
JetSuite provides efficient operations, acute attention to detail, acclaimed customer service, and industry-leading safety practices. Offering a WiFi-equipped fleet of aircraft across the US, JetSuite was recently ranked #1 in light jet utilization by ARGUS. Under the leadership of CEO Alex Wilcox, a JetBlue founding executive, JetSuite is IS-BAO certified and ARGUS Platinum rated, the highest possible safety rating in the private jet industry. Contact us via the form above for a custom quote or to find out how to get our lowest private aviation rates by becoming a SuiteKey Member.
Always enjoy flying privately. Exciting from planning a trip to booking to flight(s). Predominantly use Stratos. Attentive and personal service. Joel Thomas was a great go to person. All subcontractors that they use have been top-notched. Only critique is I obtained better final pricing of the flight when I received quotes from other charter companies. Seems to be an industry thing about competitive pricing in order to obtain the best price. Yes I would recommend Stratos.
Not only do we hold every single flight to a higher standard than most, we ensure those demands are consistently met and exceeded on a regular basis. Focused on delivering a truly flawless travel experience, we review everything — from the history of the aircraft and the actual experience of each crew member, all the way to the key personnel in the organization. Each flight is reviewed multiple times to make sure that you are provided with a level of comfort and assurance that only comes with being Skyjet Approved. This designation, reserved for the top 25 percent of private jet operators, requires an unwavering commitment to the rigorous safety standards upheld by our operators and our entire organization. Our commitment to safety never stops.
Whether you travel frequently for business or only occasionally for pleasure, chances are you've considered hiring a charter plane. When you fly on a private plane, you can skip the long wait times and baggage checks of a commercial flight. Your departure and return times are determined by your schedule, and in some cases you can even book a same-day flight! Here are some of our most commonly asked questions regarding private flights:
As private jets are constantly moving between locations, the guide prices provided below are based on various data sources relating to the aircraft's last known position. Due to this, not ALL available aircraft are included within the search results. So please contact one of our charter experts for a fixed quotation, as they are aware of all aircraft available in specific locations at any given time.