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Polo top
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The ATO has released two new videos aimed at SMSF trustees, covering the arm's length rules, and things to consider when setting up an SMSF.
Category Archives: Video
ATO SMSF videos: individual v corporate trustee, declaration
The ATO has published two new videos aimed at SMSF trustees, this time covering the differences between individual and corporate trustees for SMSFs, and the SMSF trustee declaration.
SMSF – individual or corporate trustees
"Deciding on the type of SMSF
ATO SMSF videos: pensions & planning for the unexpected
The ATO has published two new videos in the continuing efforts to improve trustee knowledge of superannuation and tax rules.
SMSF planning for the unexpected (relationship breakdown, incapacity, death)This video encourages SMSF trustees to think ahead when setting up …
ATO SMSF trustee videos: investment strategy & loans
The ATO has published two new videos aimed at educating SMSF trustees. Given the ATOs goal of providing "new online tools and resources for SMSF trustees and auditors" contained in the recently published ATO Corporate Plan we can expect to…
Superannuation Clearing House: ATO How-to Videos
The ATO has uploaded several videos explaining how to use the Small Business Superannuation Clearing House, which recently moved from the Department of Human Services to the ATO.
According to the ATO the Small Business Superannuation Clearing House is:…
Video: ATO – SMSF Annual Obligations
For those who missed it, the ATO recently published this video about an SMSFs annual obligations:
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South Boston News & Record
and Mecklenburg Sun
Poplar Creek Project envisions affordable, eco-friendly housing
02/18/19 - 8:20 am
Senate passes up Friday vote on sales tax bill
02/15/19 - 12:18 pm
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02/14/19 - 7:46 am
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02/18/19 - 8:18 am
- More A&E
Tom McLaughlin
Private lies
05/20/10 - 7:47 am
Now here's a sentence worth quoting, from the May 7 Richmond Times-Dispatch, on Gov. Bob McDonnell's plans to privately finance the $2 billion reconstruction of U.S. 460 from Suffolk to Petersburg: "Past construction proposals called for tolls as high as $13.20 per vehicle and also required $500 million to $1 billion in public funds, but Virginia has no state or federal money to build the four-lane, limited-access road."
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05/19/10 - 7:36 am
Priority review
05/13/10 - 8:00 am
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05/12/10 - 7:48 am
You might think an oil spill that threatens to ruin the health of the Gulf of Mexico for years to come would cause people to rethink their advocacy of drilling off America's coasts ... and whaddaya know, in some cases it has.
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On April 15, members of the Executive Committee of the Virginia Tobacco Commission approved $200,000 for a study of the socioeconomic impacts of uranium mining.
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04/21/10 - 2:16 pm
Last week the Virginia Tobacco Commission all but cut a check for a study of uranium mining's socioeconomic impacts in Southside Virginia, thus drawing closer the day when a decision is made — or not made — on lifting the state's mining moratorium. Funding the study is an unusual move by the Tobacco Commission, which could stand more scrutiny of its own, but it's hardly surprising inasmuch as the leaf panel claims a broad mission to revitalize Southside's economy. Besides, what's another $200,000 when you've already burned through half a billion dollars?
Overdue tribute
04/08/10 - 8:01 am
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BodyFuel™ Creatine Powder from BauerNutrition is truly a cutting-edge brand-new Creatine Monohydrate Supplement Powder, produced by industry-leading specialists Advanced Health. BodyFuel™ Creatine Powder from BauerNutrition will certainly deliver worldwide, according the the BauerNutrition main site delivery info, so it can be purchased from the BauerNutrition official site and shipped to Grenada.
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If you have actually chosen that you are going to purchase a Creatine Monohydrate Powder, I would advise you buy from the main supplier. Personally, I have actually done just that, and have found the buying procedure safe, experienced and with a great level of customer service.
I make certain there are a great deal of people who choose to keep their Creatine Monohydrate Supplements items to themselves. It's not generally something you talk about with everybody. The main supplier has a complete privacy disclosure significance, your individual payment and identity information are not shown everybody.
Customer support is great. If you have any issues with your BodyFuel™ Creatine Powder, simply email them initially. I got an action in hours. You can constantly get in touch with the numbers provided.
Exactly what to Consider Prior to Buy Creatine Monohydrate Supplement Powder from Grenada?
Prior to searching for Creatine Monohydrate Supplement Powder on the internet, you should know precisely what item you're looking for. Get in the item name into your web browser's search box to get begun examining sites that offer the item. Evaluation a minimum of three sites that use the item and pay close attention to cost, quantity and shipping fees. Search for business who are better to your shipping address. In some cases, you can catch a break in shipping charges with picking closer business. Different sites require various shipping costs and some may be less costly than others. Decide exactly what quantity you require, make your choice and place your order, supplying the significant info.
Check out the Label Carefully & Take the Suggested Dose
Labels on Creatine Monohydrate Supplement Powder product need to include the following information: statement of identity, net amount of contents, directions for use, a supplement truths panel, noting serving size, amount and active ingredients, other ingredients in coming down order of predominance, and the name and place of business of the manufacturer, packer or supplier. Constantly follow the manufacturer's daily suggested dose - more isn't really always better. If a manufacturer states to take 2 capsules per day, that's all you must take. If you take several supplements, check to see if you are getting the same component from several sources to make sure you don't exceed the suggested everyday allowance for any individual component.
How it Works
In order for a muscle to contract, adenosine triphosphate (ATP) should break off a phosphate group, leaving behind ADP (adenosine diphosphate). The only issue with this is that our body can not use ADP for energy. The solution? ADP takes a phosphate from your body's store of creatine phosphate (PCr) to form more ATP. Supplements with creatine serves to increase creatine shops and PCr availability in the body, resulting in faster ATP formation. Bottom line: The more PCr you have, the more work you can accomplish prior to tiredness sets it.
Taken appropriately and regularly, creatine can be one of the most effective supplements for increasing lean body mass and improving body composition, strength, and high-intensity efficiency.1,2 Yet misconceptions and misinformation about security and potential side effects still pet dog this supplement. Is it safe? Does it trigger weight gain? Is it damaging to your kidneys?
If you're starving for responses, you've pertained to the right place. Here's a take a look at six typical misconceptions about creatine, and the real fact behind them.
There have actually been numerous studies done to attempt and identify which dosage of creatine will give athletes greater performance levels. Most people research studies done on creatine utilized roughly 20-30 grams per day. These doses have been consumed in 4-5 equivalent doses daily for 5-7 days (loading stage).
Lots of athletes choose to utilize the powder type of the substance, which can be mixed in with fluids and is rapidly directed into the blood stream. Creatine is finest absorbed when taken with carbs. The best time to take creatine is directly after an exercise - this is when your muscles are most receptive to absorbing creatine from the blood.
Creatine Monohydrate Powder Shipping to Grenada?
You do not have to stress over Creatine Monohydrate Powder shipment to your address since currently Creatine Monohydrate Powder shipment is available to all areas or cities throughout Grenada.


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"I'd never deny that working with Francis hasn't been a big help to me because I've learned a great deal from him," Cage said. "He's one of the few directors around who enjoys making unpredictable choices and I like to experiment too so we work well together. Working with him on 'Peggy Sue Got Married' was the most satisfying thing I've done as an actor and I consider it my best performance by far."
Cage's "Moonstruck" co-star Cher shared his opinion of his work in "Peggy Sue," which found him portraying a character who ages from cocky teen-ager into middle-aged failure, and her admiration for his performance led her to urge Norman Jewison to cast him in "Moonstruck."
"Cher and I both admired Nicolas' work in 'Peggy Sue Got Married,' " said Jewison, "but the main reason she felt he was right for the part is because, like the character of Ronnie, Nicolas struck her as a tormented soul." "I was attracted to the romantic element in 'Moonstruck' because I think I am a romantic," Cage said, in explaining why he took the part. "There haven't been that many great romantic films--'The Graduate' and 'Wuthering Heights' come to mind--and I think we need more of them. Even though romance isn't always a fun thing to go through, the things men and women experience through each other are utterly mystical and illusive.


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12 Stories You Need To Know Today
25 October, 2012
Dory Carr-Harris, PSFK
In Brief
Natalie Portman mascara ad banned for being 'fake,' Zynga fire 5% of employees and eBay and FedEx team up against Amazon.... Links to start your day with.
Google brings Street View to the Grand Canyon. CNet
Taylor Swift sells her new album at Papa John's. Ad Age
Did Natalie Portman's eyelashes look too good in Dior ad? Brand Republic
British beer sales plummet. TelegraphUK
Sadly, lap dances don't qualify as 'art'. Guardian
New media artists are the unpaid R&D arm of agencies. Flong
Square launches in Canada. Engadget
Zynga drops a bunch of games along with 5% of their employees. Wall Street Journal
Billionaire donates $100 Million to Central Park. NY Times


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Haverford School didn't go far to find John Nostrant's replacement as the head of its lacrosse program.
The school announced Monday that Brendan Dawson will take over as Haverford School's director of lacrosse and assistant athletic director, effective Aug. 27.
Dawson will work on Nostrant's staff this spring, his final season at the helm of the national powerhouse, then succeed Nostrant in the top job in 2019-20.
"We are excited about Brendan joining the Department of Athletics at Haverford," said Mike Murphy, who replaced Nostrant as Director of Athletics, in a school statement. "He has led and elevated three college lacrosse programs and earned conference coach of the year recognition at each. Brendan's desire to impact student-athletes at an early stage in their development and to further the tradition of Haverford School lacrosse made him a terrific fit for our program. We are fortunate to have such an outstanding coach to work with our boys, and to continue the strong legacy of Coach Nostrant."
Dawson spent two seasons at Haverford College, breaking a seven-year postseason drought in 2018 to earn Centennial Conference Coach of the Year honors, despite a 7-8 record. Before that, the 2004 graduate of Salisbury spent five seasons at Widener, where he was the MAC Commonwealth Coach of the Year in 2014 and 2016. He also helped launch the program at Aurora University in Illinois.
"My family and I are eager to join The Haverford School community in this exciting new role," Dawson said. "I would like to thank (Head of School) John Nagl, Mike Murphy, and John Nostrant for their faith in me. I am humbled by the opportunity to learn from Coach Nostrant in this coming year, and motivated to work at continuing the tradition of excellence that is Haverford School lacrosse."
The spring will be Nostrant's 28th and final season at Haverford School, where he has cemented a reputation as one of the nation's finest lacrosse coaches. He stepped down in June to spend more time watching his kids play college lacrosse.
"We were fortunate to interview and meet with some exceptional candidates and Brendan handled himself very well," Nostrant said in the statement. "He has a lot of experience for a relatively young guy. I am excited for him and feel very strongly that he will be a great fit for Haverford and that he and his family will be a part of the community for years to come. He is going to do great things here."
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text:
Chapter 836: Bringing a Third-Wheel on Vacation (7)
Translator: Noodletown Translated Editor: Noodletown Translated
"Pregnant? Oh my god, are you kidding me?" Jiang Xiaowei almost went crazy.
"Girl, this is an early pregnancy test, and there are two lines on it."
"But the second line isn't that obvious, how can you be so sure that I'm pregnant?" Jiang Xiaowei was speechless.
She hadn't been with Wei Liao for very long…
It had only been 20 days since they moved in together.
This morning, she noticed that her usually on-time period was a day late.
For some strange reason, she bought an early pregnancy test, and…?
"This should be accurate." Huo Mian nodded.
'Holy crap… what am I supposed to do now?" Jiang Xiaowei didn't seem ready to be a mother.
"Girl, isn't it a good thing? Why do you look like something evil had just fallen onto your lap?" The truth was, Huo Mian admired her.
Wouldn't it be great if she was the one who was pregnant…?
"How is that a good thing? I don't even know what's going to happen to us. Plus, I'm not ready yet."
"If you're not ready yet, why didn't you use protection?"
Jiang Xiaowei was a straight 'A' student and had a Ph.D. in psychology; where was her common sense?
"I did… there was just that one time when I got drunk. He pounced on me without a condom, just that once…"
"Sometimes these things come mysteriously…" Huo Mian replied quietly.
"So, are you certain I'm pregnant? It's only been a little over ten days, do you think it's really possible?"
Jiang Xiaowei was still unwilling to believe that this was actually happening….
"There's something else we can do to make sure. Come with me to the OB/GYN department. You can get a blood test to check your progesterone, then we'll know for certain."
"That sounds good, let's go. Take me there."
Before Huo Mian could say anything else, Jiang Xiaowei pushed her towards the elevator.
– Upstairs, in the OB/GYN Department –
Huo Mian sat with Jiang Xiaowei as her colleague drew her blood, and then she told the doctor at the lab to speed up the results.
– 40 minutes later –
A miserable Jiang Xiaowei came out with the lab report…
"This baby came so f*cking suddenly."
"It means you guys were meant to be. Don't tell me you plan on getting an abortion?"
"That idea did cross my mind…"
"Don't do that, you can give birth to it and I'll raise it. You can't get an abortion. After all, it's a life," Huo Mian said with puppy dog eyes.
"Hahaha… I was kidding. Both mine and Wei Liao's parents are going to be super happy… His parents don't like me because they think I'm too straightforward… but I don't like them either. My pregnancy might be able to shut them up."
"Yeah, since you have plans to spend your life with Wei Liao, you need to keep this child."
"I need to see the reaction on his face first." The truth was, Jiang Xiaowei was a little worried.
Usually, before a man was ready to marry a woman, he wouldn't like it if she got pregnant.
They didn't have a wedding yet, nor were they officially registered. Although that engagement party caused quite a stir, at the end of the day, it was only a party.
However, Wei Liao wasn't that young anymore; he was 28 years old, even older than Su Yu.
Maybe he'll be happy that Jiang Xiaowei's pregnant.
After confirming her pregnancy, Jiang Xiaowei felt a little complicated – a mixture of happiness and sorrow.
Huo Mian, who admired her, kept telling Jiang Xiaowei to take good care of herself.
She even ran to the pharmacy and bought her some folic acid.
"Take this until the end of your first trimester, it's good for your baby."
"Thanks, my genius doctor." Jiang Xiaowei pinched Huo Mian's cheek.
After Jiang Xiaowei left South Side, she went straight to Wei Liao's company.
The Wei Family had a big business; they started in the steel industry and were now in the chemical materials business.
Unlike Qin Chu, Wei Liao wasn't the president of his family business, nor did he wield as much power as Qin Chu did.
However, he was still the executive vice president.
"Wei Liao…" Jiang Xiaowei called out as she walked towards his office.
"Wei, what brings you to the company?"
"I have good news and bad news, which one do you want to hear first?"
"Bad news."
"The bad news is that I think I'm pregnant."
Wei Liao was immediately flabbergasted…
"What's the good news then?"


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Tag Archives: Publishing
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Posted in Legal Marketing Tagged Advantages, display, open, Publishing, Software, Source, Website, wordpress Leave a comment
Gutenberg is the future of legal publishing
Watching WordPress cofounder, Matt Mullenweg deliver his annual State of the Word at WordCamp US on Saturday afternoon from Nashville there was little question that Gutenberg is the future of legal publishing. Beginning with the WordPress text editor (unchanged for a … Continue reading
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Law firm blog publishing practices hurting lawyers and the law
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Alexander on Publishing Peter Pindar: Production, Profits, and Piracy in Georgian Satire
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Publishing Contracts: Where Lies the Danger for Indie Publishers?
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Yeah I'm the guy who they asked to do magic.
It was awesome - she walkes in and says "now teacher is gunna do some magic" - go on, do some magic.
I must say it's fun and one of the countless time the bloody woman came into my classroom and interrupted my lessons.
...not even going to get into the drama I had with them regarding the living conditions.
Glad I got out of there, can't recommend it. Guess there are worse jobs out there but this is definitely a school to avoid.
I worked for the school, the owner is a bitch and her husband is her bitch. They will harass you, berate you and generally treat their teachers like shit. And yeah they will talk smack about the previous teachers.
When I first went for an 'interview' with them the owner 'broke down' and started crying - why do they all hate us - we are good people. Red flag much?
Luckily I was a part time teacher so the moment they started telling me crap like I need to make more effort, from a foreign country on holiday by the way I was like okay - see ya. Best of luck. They underpaid me anyway.
Worst school I ever worked at - God help anyone who signs a contract with them!!
Oh shit, and the bullshit continued I almost forgot.
The bloody owner went to the same gym as me. After I quit with no harsh words or drama she goes and speaks to the gym owner for a while. When I'm getting the card back she tells me I owe her 200 bucks!! What WTF??
I paid a small fortune for the gym contract and hardly go, I asked why and she just threw the card at me and waved me off like a dog. Previous to that she was super friendly, almost too friendly.
Yeah - ooh - why do that all hate me - that's why!!
You are a bitch!!!
i know someone who was 'hired' via online interviews, everything seemed great and actually made quite a fast effort to get there, turned down another job, waited... and they never returned his calls or messages for 2 days. Finally he lets them know he's in a hotel and needs to know what's happening and gets some message saying that they decided he wasn't suitable. Pressed for some explanation they seem to suggest a female manager decided she was suspicious or disliked some personal issue (like him being married or something like this).
To my mind it seemed very vindictive and both the people (who might be Y and B??) but they came across as fickle, snotty, nitpicky and then there was that 'personal dislike' part which squares with your account of the 'firing' turning into mean-spirited personal attacks and lies and suspicions etc.
It's interesting I stumbled across this post and yes it was Real English Foshan and yes I believe I might even know the people you mentioned (but not the teachers).
This "Y" couldn't have come at a better time, I just ran out of salt.
So much effort and time wasted trying to discredit individuals for the sake of face, I will never understand Chinese people.
Real English - Foshan China
This school is claiming to have hired and fired me because I'm a terrible unqualified teacher due to a dispute with another school.
In reality I declined multiple requests to work for them because of all the horror stories I was told by people who have.
I know three people who worked for them including my wife. All three were underpaid and over stressed. I drew the line and told my wife to quit when she was been told she needs to make more effort in some regard, it was just insulting, annoying and unnecessary. It's a kindergarten and she is amazing with young kids who are usually in tears when she leaves.
Anyway one of the teaching assistants ('B') left Real English to help her one of her family members start a school. She has about 13 years experience teaching - 10 in government schools and 3 in Real English.
They were upset to see her go and proceeded to slander her to all the parents by calling them individually to try keep them from leaving. One day I was helping B with an opening promotion and she looked really upset. I asked her if she was okay and she said no, the previous schools owner (Y) is calling everyone she can and telling her how terrible B is.
A couple of days later I got a call from Y. She proceeded to slander B for a half an hour, accusing her of stealing students and equipment from the school including desks and chairs. I had seen the new school has top class facilities including smart boards and free espresso machine. Everything brand new.
I watched from a distance while the owner of Real English, 'Y' went on a tyrannical rampage to do as much damage to CF as possible and stress out 'B' as much as she could. Including standing outside the school and telling parents that my wife and I were hired and fired from their school, are terrible teachers and unqualified to boot. To be qualified in this country you need to be an English speaker. That's it. I do however have all the requirements to be a 'foreign expert' in China and my wife is completing a masters.
This slander campaign has gotten out of hand and I'm hoping this review will end the drama because if the slander continues I'm gunna ask my friends to share their storieswhich wouldn't be pretty.
'Y' bad mouths all previous teachers and denies that she is doing or has done anything wrong to the death. I have seen too much to believe this.
I was a bystander so always had a neutral opinion but been brought into the line of fire had made me react.
I don't take kindly to me or my kin been slandered for no good reason.
It's ludicrous.


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Hazel Pace - 01538 360886 - M: 0793 069 0002 - firstname.lastname@example.com Description 32 Count Partner Dance. Start in Closed Western, Gent facing LOD, Lady facing RLOD Music Things To Do In Wichita - Mark Chesnutt. 83/166 BPM CD: Rollin' With the Flow ~ Get it Here Intro: 16 Counts.Choreographed by
GENT LADY 1-8 Mambo Step, Side Shuffle 1/4 Turn CW, Rock Recover Side, Weave.1&2 Rock forward on left, recover on right, Right mambo back. step back on left. 3&4 Side shuffle right, left, right making 1/4 turn Side shuffle left, right, left to face ILOD. to face OLOD. 5&6 Rock left over right, recover on right, Rock back on right, recover on left, right to right side. left to left side. 7&8 Cross right over left, left to left side, Left behind right, right to right side, right behind left. cross left over right.
9-16 Side Rock, Recover 1/4 Turn, Step 1/2 Pivot Step, Step Lock Step, 1/4 Turn Rock Recover.1-2 Rock left to left side, recover 1/4 turn right. Rock right to right side, recover 1/4 turn left. 3&4 Step forward on left, 1/2 pivot right, Step forward right, 1/2 pivot left, step forward right. step forward on left. (No hands). 5&6 Right step lock step. Left step lock step. (Inside hands, facing LOD). 7-8 Make 1/4 turn right rocking left to left side, 1/4 turn left rocking right to right side, recover on left. recover on right. (Facing in Closed Western).
17-24 Full Turn CW on the spot in Closed Western, 4 Counts, Rock Recover 1/4 Turn, Walk R-L (Lady 1/2 Turn Right, Step Back).1-4 Make full turn CW rocking from left to right as you turn, left, right, left, right. Lady right, left, right, left. 5&6 Rock back on left making 1/4 turn left, Rock back on right making 1/4 turn right, recover on right, step forward on left. (LOD). recover on left, step forward on right. 7-8 Walk forward on right, left. Make 1/2 turn right, stepping back on left, (lady goes under her own right arm). step back on right. (RLOD).
25-32 Shuffle, Side Rock Recover 1/4 Turn, 3/4 Triple Turn, Shuffle.1&2 Right shuffle forward, Left shuffle back. 3-4 Rock left to left side, recover 1/4 turn right. Rock right to right side, recover 1/4 turn left. 5&6 Make 3/4 triple turn right on left, right, left. Make 3/4 triple turn left on right, left, right. (No hands, turn to face partner). 7&8 Right shuffle forward. Left shuffle back.
|Every effort
has been made to ensure these cue Sheets
are accurate, Should you find any errors,
Please let me know via e-mail thank you...... Robert
|January 2009


text:
Canopy Design, Canopy Installers Canopy Installers Near Me Car Canopy Covered Walkway Glass Canopy Waterproof Brick Wall Wooden Floor: 2018 canopy installers collection
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Quite often, business owners are so busy doing back office work (such as accounting, bill payment and payroll functions) that they run out of time to effectively and efficiently focus their talents. Their time is best spent marketing, providing superior client service and attending to other executive duties that are absolutely essential to the growth and ultimate success of their businesses.
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According to Forbes.com, Super Bowl viewers traditionally load up on millions of pounds of less-than-healthy foods during the big game—including ribs, pulled pork, tortilla chips, nuts, popcorn and bacon—all washed down with beer (the Super Bowl beverage of choice). If you are trying to stick to your New Year's resolution to eat better, consider a few healthy substitutes for the traditional Super Bowl eats:
The combination of running a business and your life and preparing for tax time can drive some people into a slight panic. But no need to get stressed if you are prepared. Now is the time to start organizing all documents required to file your tax return.


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Our group has previously demonstrated that short-term treatment with esmolol reduces left ventricular hypertrophy (LVH) in spontaneously hypertensive rats (SHRs). The present study aimed to assess the molecular mechanisms related to this effect. Fourteen-month-old male
Left ventricular hypertrophy (LVH) is a compensatory response to chronic arterial hypertension. It is an independent risk factor for cardiovascular morbidity and mortality including heart failure, coronary artery diseases, stroke, arrhythmias, and sudden cardiac death [
Esmolol is an ultrashort-acting cardioselective
Our group previously demonstrated that short-term treatment (48 hours) with esmolol reduces LVH in spontaneously hypertensive rats (SHR) [
It is increasingly evident that redox-dependent modifications in cellular proteins and signaling pathways play an important role in cardiac hypertrophy [
In this study we tested the hypothesis that esmolol could lead to early regression of LVH, at least in part, through MAPK-mediated regulation of NFATc4 and NF-kB transcription factors. Therefore, the present study aimed to assess whether short-term treatment with esmolol can reverse left ventricular hypertrophy in aged SHR hearts and whether these effects are mediated through regulation of Akt/NF-
The study animals: 14-month-old male spontaneously hypertensive rats (SHR) (
Systolic arterial pressure and heart rate were measured using the tail-cuff method with a photoelectric sensor (Niprem 546, Cibertec, Madrid, Spain). Several determinations were made at each session before and after treatment (0, 12, 24, 36 and 48 h), and the findings were considered valid if 10 consecutive measurements were within 10 mmHg of each other.
Left ventricular tissue samples were homogenized using a TissueLyser LT system (QIAGEN, Hilder, Germany) programmed with 50 s−1 oscillation for 4 min in a lysis buffer containing 20 mM Tris-HCl buffer (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM EDTA, 1% Triton X-100, 20 mM sodium orthovanadate, 1 mM sodium fluoride, 1 mM PMSF, and 1% protein inhibitor cocktail acquired from Sigma-Aldrich (Madrid, Spain). Homogenates were centrifuged at 10,000 ×g for 2 min at 4°C and the supernatant was stored at –80°C until analysis.
Immunoblotting with left ventricular homogenates was used to assess the total (T-) and phosphorylated (p-) levels of NFATc4, AKT, ERK, NF-kB, JNK, CREB, and SERCA. Forty milligrams of left ventricular protein was separated by SDS-PAGE electrophoresis on 12% gel using the Mini-Protean Tetra system (Bio-Rad, Madrid, Spain) at 100 V and room temperature. Separated proteins were transferred onto an Immunoblot PVDF membrane (Bio-Rad, Madrid, Spain) for 1 h at 120 V and 4°C. The efficiency of protein transfer was verified using reversible protein and a stain kit (MemCode, Thermo Scientific, Madrid, Spain). Primary antibodies against total (T) and phosphorylated (p) NFATc4 (Santa Cruz Biotechnology, Germany; dilution factor [DF] = 1 : 200) and p-AKT (DF = 1 : 1000), p-ERK (DF = 1 : 2000), p-NF-
Total protein carbonyls in homogenized left ventricular tissue were quantified using a simplified 2,4-dinitrophenylhydrazine (DNPH) spectrophotometric assay [
Left ventricular total thiol levels were assessed using the 5,5′-dithiobis (2-nitrobenzoic acid) assay [
Total antioxidant capacity of left ventricular homogenates was assessed using the CUPRAC-BCS assay [
Catalase activity was assessed using the Amplex Red assay (Invitrogen, Spain) adapted to a 384-well plate. Absorbance at 560 nm was read after 30 min of reaction at room temperature in a multimode microplate reader (Synergy HT, Bio-Tek, VT, USA). Catalase activity was expressed as U/mg protein, calculated from the CAT standard curve (0–2 U/mL).
Protein content was assessed using the Coomassie blue-based microtiter plate assay according to the manufacturer's recommendations (Bio-Rad, Madrid, Spain). Absorbance was measured at 595 nm in a Synergy HT Multimode Microplate Reader (Bio-tek), and bovine serum albumin was used as the standard.
The results were expressed as the mean ± SEM. The parameters were compared using repeated measures analysis of variance (physiological parameters) or single-factor (rat) analysis of variance (western blot analysis and oxidative stress parameters). A post hoc Bonferroni correction was applied. Statistical significance was set at
Values of physiological parameters are shown in Table
Systolic blood pressure and heart rate in WKY, SHR, and SHR-E.
|Time treatment (hours)
|SBP (mmHg)
|HR (bpm)
|126 ± 21
|310 ± 20
|135 ± 18
|317 ± 15
|119 ± 11
|324 ± 21
|121 ± 22
|319 ± 19
|127 ± 23
|320 ± 12
|190 ± 20
|322 ± 15
|217 ± 25
|311 ± 11
|198 ± 21
|324 ± 18
|223 ± 12
|312 ± 20
|205 ± 23
|313 ± 16
|199 ± 31
|320 ± 13
|137 ± 15
|229 ± 12
|139 ± 28
|231 ± 10
|145 ± 11
|219 ± 18
|143 ± 10
|220 ± 13
WKY: Wistar-Kyoto rats; SHR: spontaneously hypertensive rats; SHR-E: esmolol-treated SHR rats; SBP: systolic blood pressure; HR: heart rate. Values are shown as mean ± SEM.
In the whole tissue left ventricular homogenates, p-NFATc4 levels were markedly higher in SHR than in WKY controls, and short-term treatment of SHR with esmolol significantly reduced p-NFATc4 levels (Figure
Expression levels of p-NFATc4 in the left ventricle. Experiments were performed in homogenates of left ventricular tissue from Wistar-Kyoto rats (WKY), spontaneously hypertensive rats (SHR), and esmolol-treated SHRs (SHR-E). Values are shown as mean ± SEM;
Expression levels of p-Creb1 and Serca2a in the left ventricle. Experiments were performed in homogenates of left ventricular tissue from Wistar-Kyoto rats (WKY), spontaneously hypertensive rats (SHR), and esmolol-treated SHRs (SHR-E). Values are shown as mean ± SEM;
p-Akt and p-NF-kB expression in the left ventricular tissue was lower in SHR-E than in SHR and treatment with esmolol reduced this expression to the protein levels of WKY (Figures
Expression levels of p-Akt (a), p-NF-
Short-term treatment with esmolol restored catalase activity to the levels in WKY control heart tissue. Compared to WKY rats and SHR-E, catalase activity was decreased in SHR animals (Figure
Left ventricular oxidative stress biomarkers: catalase activity (a), protein carbonyls (b), total thiols (c), and total antioxidant capacity (d). Experiments were performed in left ventricular homogenates from Wistar-Kyoto rats (WKY), spontaneously hypertensive rats (SHR), and esmolol-treated SHRs (SHR-E). Values are shown as mean ± SEM;
We previously reported that short-term treatment with esmolol produces early regression of left ventricular hypertrophy (LVH) in SHR [
Induction of the calcineurin-NFAT pathway is a common hallmark of pathological hypertrophy [
Esmolol is an unique cardioselective
Calcineurin-NFAT and PI3K/Akt signaling pathways are interdependent and together orchestrate the cardiac hypertrophic response [
The Akt pathway is actively involved in the regulation of NF-
In a previous study, our group has demonstrated that, compared with SHR, plasma superoxide anion scavenging activity (including superoxide dismutase) was more pronounced in SHR-E and this was associated with an improvement in nitric oxide availability measured as nitrites/nitrates [
Left ventricular hypertrophy (LVH) is probably the most visible manifestation of hypertensive organ damage [
In conclusion, our findings show that short-term administration of esmolol reverses LVH in adult SHR through inhibition of Akt/NF-
The authors declare that they have no conflicts of interest.
This study was supported by Spanish Health Ministry (Fondo de Investigaciones Sanitarias) under Contracts FIS 13/01261 and FIS 16/02069 and Fondos Feder.


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The role of agriculture in Ghana's economy cannot be understated. From food items for our consumption and export, jobs and employment and many others.
History of Ghana tells us the significant role it has played from colonial times right down to independence.
Despite its many benefits as it is often characterized as the mainstay or backbone, it seems we have been comfortable with this and we as a people have not paid significant attention to the other sectors of the economy, particularly industry.
On PM EXPRESS BUSINESS EDITION, Philip Nanfuri explores a critical component of agriculture - financing.
Are financial lenders shying away? If that's the case then why? What can be done about it?
The government in the 2019 budget says it is going to operationalize a system for agricultural lending, GIRSAL. Will it work and what exactly is it?
Click on the video below to follow the discussion:
How much do banks lend to the agricultural sector? A whopping 3.9% of all loans given by banks go to the agricultural sector. Sectors such as Commerce and Finance are pulling much more credit. Why is this so?
Growth rates in the sector were abysmal but have picked up? Can we sustain it?
Agriculture's share of GDP has declined over the course of time with services overtaking it, is this a cause for concern?
The agricultural sector's share of GDP in some developed countries is low but there's enough to feed its nation and export.
Have your say
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- Huawei commits to becoming a market leader in Ghana; unveils new Y7 Prime


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Fixed-term Operations AdministratorListed seven days agoat VisionWest Community Trust
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Edwardian Mahogany Billiard Cue Stand c.1910.
Fine quality Edwardian era mahogany billiard cue stand c.1910. 18″ diameter revolving stand to hold 18 cues. Pierced top with moulded edge raised on turned supports around a matching centre column all with ball finials. Matching base revolving on a moulded plinth raised on turned feet. Excellent colour and condition, cleaned and polished in our workshops.
Dimensions: width 18″, depth 18″, height 40″.
Year: c.1910.
Price: £745
Stock Number: 32616


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Nature of Business
Legal Status of Firm
Private Limited Company
Manufacturer of fancy sarees, designer sarees etc.
Sakambhari Silk Mills Private Limited
No. 1012 & 1031, Anupam Tex Market, Behind Millenium Textile Market, Ring Road,
Surat-395002, Gujarat, India
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