text:
Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes
- Autores
- Leal Denis, Maria Florencia; Incicco, Juan Jeremías; Espelt, Maria Victoria; Verstraeten, Sandra Viviana; Pignataro, Omar Pedro; Lazarowski, Eduardo R.; Schwarzbaum, Pablo Julio
- Tipo de recurso
- artículo
- Estado
- Versión publicada
- Año de publicación
- 2013
- País
- Argentina
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- Repositorio
- CONICET Digital (CONICET)
- Descripción
- Background: The peptide mastoparan 7 (MST7) stimulated ATP release in human erythrocytes. We explored intraand extracellular processes governing the time-dependent accumulation of extracellular ATP (i.e., ATPe kinetics). Methods: Human erythrocytes were treated with MST7 in the presence or absence of two blockers of pannexin 1. ATPe concentration was monitored by luciferin–luciferase based real-time luminometry. Results: Exposure of human erythrocytes to MST7 led to an acute increase in [ATPe], followed by a slower increase phase. ATPe kinetics reflected a strong activation of ATP efflux and a low rate of ATPe hydrolysis by ectoATPase activity. Enhancement of [ATPe] by MST7 required adhesion of erythrocytes to poly-D-lysin-coated coverslips, and correlated with a 31% increase of cAMP and 10% cell swelling. However, when MST7 was dissolved in a hyperosmotic medium to block cell swelling, ATPe accumulation was inhibited by 49%. Erythrocytes pre-exposure to 10 μM of either carbenoxolone or probenecid, two blockers of pannexin 1, exhibited a partial reduction of ATP efflux. Erythrocytes from pannexin 1 knockout mice exhibited similar ATPe kinetics as those of wild type mice erythrocytes exposed to pannexin 1 blockers. Conclusions: MST7 induced release of ATP required either cell adhesion or strong activation of cAMP synthesis. Part of this release required cell swelling. Kinetic analysis and a data driven model suggested that ATP efflux is mediated by two ATP conduits displaying different kinetics, with one conduit being fully blocked by pannexin 1 blockers. General significance: Kinetic analysis of extracellular ATP accumulation from human erythrocytes and potential effects on microcirculation.
- Idioma
- inglés
- OAI Identifier
- oai:ri.conicet.gov.ar:11336/2772
- Enlace del recurso
- http://hdl.handle.net/11336/2772
- Nivel de acceso
- Acceso abierto
- Materia
Bioquímica y Biología Molecular
Ciencias Biológicas


text:
Class A CDL Driver Lease Purchase
Company: Soar Transportation Group
Location: Springfield
Posted on: February 10, 2019
Job Description:
BRAND NEW LEASE PURCHASE PROGRAM BRAND NEW TRUCK NO MONEY DOWN & NO CREDIT CHECK --- Drive a Brand New 2019 Freightliner Cascadia --- Bumper to Bumper Maintenance Program --- EW4 and TC4 coverage --- 4 year - 450,000 mile chassis warranty, 4 year - 500,000 mile engine warranty --- Average Gross Annual Pay: $200,000 PLUS per year Now more than ever, great people are needed to get the job done. At Soar Transportation Group, our vision is to be the best in the industry. We want you to be a part of it. Soar Transportation Group knows what's important to you & we have built our company around providing our Contractors with the best. We also provide customer service team who respects our Contractors for what they do and who they are. --- Average weekly miles 2,560 --- Average Deadhead 7.5% --- Pay is 70% of gross linehaul revenue --- Average gross pay annually $200,000 plus a year --- 100% pass thru fuel surcharge --- Company paid reefer fuel --- Weekly Settlements --- Company fuel network/discounts 100% pass thru (average 29-- per gallon in 2017) --- EFS card for fuel, def, reefer and scales (all charges will be applied directly to trip & deducted at time of payment) --- Single Source Dispatch (Independent Contractor dispatch) --- Blue Chip customers --- No Touch Freight --- Consistent miles --- No trailer rental (trailer interchange agreement) --- Discounted service rates & prices INDEPENDENT CONTRACTOR QUALIFICATIONS : --- 2290 Must be current --- Current Safety Inspection and emissions --- Minimum 1 year driving experience EQUIPMENT REQUIREMENTS : --- 2011 or newer --- Must be California Carb compliant --- Max weight 19,000 lbs --- 5th wheel height 47.5 inches INSURANCE REQUIREMENTS: --- Physical Damage Insurance --- Bobtail/Non-trucking liability --- Occupational Accident Coverage --- General Liability Coverage
Keywords: Soar Transportation Group, Springfield , Class A CDL Driver Lease Purchase, Professions , Springfield, Ohio
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Where they filmed the cult movie
Good movie - always a reason to travel, even in imagination. But you can actually ride in the footsteps of your favorite heroes or plunge into the atmosphere of the legendary film.
It is clear that at a time when computer technology helps to create artificial worlds, subordinate only imagination of their creators and the thickness of the wallet customers. But even the most notorious kibertvortsy draw inspiration from the real world completely.
Harry Potter
For the filming of Diagon Alley was partially used Leadenhall Market (Leadenhall) in London. This is a huge food market, existing since the Middle Ages to the present day. Its ornately decorated Victorian covered shopping arcades look very impressive. In the first film in the Harry Potter corner shop Leadenhall served as the entrance to the Leaky Cauldron, through which, referring to the right brick wall, you can get into Diagon Alley.
Hogwarts field operations. For nearly 700 years, the castle owned by the Duke of Northumberland Eniko. The current, 12th in a row, the owner went to the second largest inhabited castle in Britain. Eniko what it means to farm on the river Ain, first mentioned in chronicles of the XI century, when within a mile of the city wall was killed Scottish King Malcolm Canmore. The medieval fortress of gloom noticed many directors of feature films: here filming "Mary Stuart, Queen of Scots", "Elizabeth", "Robin Hood, Prince of Thieves" with Kevin Costner and, of course, Harry Potter.
Forbidden Forest. Filming Locations: Black Park, Iver Heath, Buckinghamshire. Most trees in this forest grow so thickly that there twilight reigns even in the bright sunny day.
Library of Hogwarts. Bodleian Library - Library of the University of Oxford, which is contested in the Vatican right to be called the oldest in Europe, and the British - the title of the largest UK book collection. Since 1610 (officially - from 1662) year is entitled to receive legal deposit of all publications produced in the country.
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First ZelectricBug Up For Sale At $45,000 – Video
The California-based Zelectric Motors recently presented the ZelectricBug, which is a 1966 Volkswagen Beetle converted to electric drive with some help from EV West.
According to Zelectric Motors, the car is in perfect shape and "No metal is cut – nothing welded on".
It has a 50 kW induction motor (HPEVS AC-50) for over 100 mph top speed, 20 kWh battery pack with range of 80 miles and a price tag of $45,000.
" Zelectric Motors has completed its electric 1966 Volkswagen Beetle rebuild, and has put it up for sale. The '66 ZelectricBug, as it's called, is powered by a 50-kW electric motor and 20-kWh battery pack, and offers 120 pound-feet of torque. It has a range of 80 miles, and is capable of at least 100 mph. The stock transmission has been rebuilt. It also has some new goodies like high-performance sway bars, custom shocks, and LED lighting. The '66 ZelectricBug is priced at $45,000."
Detailed description of vehicle up for sale:
"++ For a complete list of features click "SHOW MORE" below ++
– Maintenance-free 100% electric 3-phase 50KW AC motor
– 80 mile range
– Double+ the original horsepower
– No metal is cut – nothing welded on
– $45,000
– 120 foot-pounds of instant torque
– top speed 100+ MPH (so far)
– 20 kWh battery pack (37 160Ah cells)
– Lithium LiFePO4 Batteries
– Battery life: 160,000+ miles – 10–15+ years
– American-made electrical components
– Fast! Weighs 1000 lbs lighter than Nissan Leaf
– 6061 Aluminium Battery Boxes
– Heavy Duty Clutch & Lightened Flywheel
– J1772 Charge Port – Charge it anywhere
– No oil. No gas. Zero emissions.
– No valves to adjust
– Huge VW parts network worldwide
– VW mechanics everywhere
– White wall radial tires
– Front Disc Brakes
– Sound Deadening throughout
– Stock (rebuilt) transmission
– Heavy duty shocks
– Digital eFuel Gauge
– Electric Ceramic Heater
– AMFM Radio/CD/MP3/USB
– LED Head/Tail lights + 3rd brake light
– Spare tire with Jack
Motor differences
– no maintenance
– no valves to adjust
– no carburetors to adjust
– no fuel, oil, air filters to replace
– no belts or mufflers to replace
– no spark plugs/wire
– no oil
– no gas
– made in america
– way more effective use of energy
– uses american made energy
– 85% of new power plants are using green energy
Driving differences
– shifting — less
– braking — less
– HP — double
– torque — instant
– motor maintenance — none
– no warm up
– this is a city car — 80mi range
– plugin at night – ready in the morning
– solar power ready
– charge anywhere 110/220v household outlet or 220 public charger
Included additional items – The Zelectric build
– disc brakes
– sway bars
– radial tires
– rebuilt transmission
– LED headlamps
– LED lights – bright and safe
– 3rd brake lamp bright and safe
– Seat belts
– HD clutch and related parts
– HD custom shocks
What's important in a classic car that we keep stock
– stock height
– nothing cut – unmolested dash
– period correct interior
– can be converted back to gas
– white wall tires
– spare tire
– manual transmission
– car is going up in value
Everything works and is in great shape
We test the car 500+ miles before releasing it
Everything is tested – what needs replacing we replace
Cool items in this car
– NEW single VW logo key for 4 locks incl motor bay
– Radio delete
– unmolested dash
– vinyl steering wheels cover
– German bumpers
– rebuilt speedometer
– stereo w usb
– New WW running boards
– New 2-tone powder-caot smoothy rims
– 1300 badge 66 only
– White wall radials new
– 66 hazard lights
– 66 wing window latches
– Clean tight headiner
– Red coco mats
– New front beam
– No paint scuffs on door jams
– Painted well w everything removed
– Color sanded / smooth
– Black CA plate
– Detachable antenna
– Porsche style headlight glass
– La Jolla Concours 2014 Best in Class
– Steve McQueen 2014 Chairmans Award
– Steve McQueen 2014 Best VW Award
– San Diego Car Show on the Bay 2014 Import Award 2nd Place
– Pacific Grove Little Car Show 2014 Big Green Zucchini Award
Options (not included)
– electric ceramic heater
– Black powder coated roof rack
– tire/wheel options
Contact: david @ ZelectricMotors (dot) com"
Categories: General, Volkswagen
Leave a Reply
13 Comments on "First ZelectricBug Up For Sale At $45,000 – Video"
Nice job on this one!
Nice looking car and great conversion.
But $45K for an 80 mile range EV with a 50KW motor? No, thanks.
Ah, someone who knows the price of everything and the value of nothing.
Spec, he did say that the 80 mile range is going at a steady 55-65 MPH.It should be able to break 100 miles in-city.
You realize that a perfect ICE beetle isn't very cheap. Right? Plus this is something that you buy because it's cool, not because you want to daily it. It's a weekend car for the Tesla owner.
So is this still a 6 volt electric system? I couldn't tell for sure since he's substituted LED lighting for many items, however there is still that 6 volt wiper motor.
Interesting about the 2.5 kw charger. People complain about the standard Leaf (and VOlt) 3.3 kw charger all the time.
Nice that he used the standardized j1772.
He's only mistaken about 2 things:
My 1964 model had a turn signal headlight dimmer, so its not 'new for '66'.
The gas gauge in the '64 was totally accurate and reliable.
One little thing: the windshield washer runs off the spare tire pressure and I didn't see it connected… Why not?
I wonder why they didn't mount the J1772 under the gas filler flap since they obviously don't need a gas cap for the non-existent gas tank. Even a 6.6kw charger would only need 2 10 gauge wires (the gnd could be immediately attached to the chassis so it wouldn't have to run in the car).
At the plug-in-america ('drive electric') event this year in rochester, ny, an older gentleman converted a 'junk' 1974 beetle (a 12 volt system by then so he didn't have any issues that way) to a Lead-Acid Series-Wound brush type 7 horsepower (!!!) 40 mile range vehicle.
He said the electric conversion was trivially easy. DBW gas pedal, (in the boot), and a PWM DC controller, and that was it. Opening up the gas flap, you saw 2 cords, one for the main lead acid charger (1800 watts) (15 amps at 120 volts, or thereabouts), and a trickle charger for another plain 12 volt battery which ran all the 1974 beetle stuff. It also ran the main contactor for the main battery so he told me that was the critical battery to keep charged, and not to run the radio or lights too much.
I asked him how much he spent on the whole job, and he said 'around $4,500'. I guess he was basically given the junk beetle, since it was maybe worth $50, and he said the vast majority of hours were spent unseizing all the wheels and making the car driveable, but that again, the 7 hp electric conversion he said was trivially easy, and he says he knows next to nothing about electricity..
I'll say this, for a person who claims to know nothing, he's got a nice 40 mile range vehicle for $4500. And nice and quiet.
I'd like more range: make it a 300 mile range model for $70,000 and he's got a sale.
Nice effort, but I wish on a 'classic' they'd stop worrying about changing the light bulbs (its a trivial amount of juice anyway), and put a decent battery in the thing so that I can drive somewhere.
I'd be a bit concerned about sending twice as much horsepower through the original drivetrain. As far as a wish list, I'd much prefer a 35 kw electric drive motor, and, with a 300 mile battery that I'd take on trips, since it wouldn't cost that much more, a 6.6 (or thereabouts) kw charger so that I wouldn't have to spend an ungodly amount of time at the public chargers.
The home charge rate can be anything, but I need something a bit faster , like 6600 watts, on trips.
I would buy a new 200 mile range New Beetle EV with DCFC…
I've personally seen this car and it is show-car quality. Easily one of the nicest EV conversions I've ever seen.
if there's anything that make me feel old quick, it's that everything remotely cool starts at ~$50k.
I didn't pay $50k for my first 3BR brick house.


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Singles from all over Rizochóri joined Swirlr. Meet them!
Are you a single man or a single woman in Rizochóri? Swirlr can change that!
Rizochóri is full of singles who want to meet a like-minded person just like you. In case you want to meet a loving partner join Swirlr and search for your other half.
Interracial Dating in Rizochóri
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The first time Baylor played Iowa State, all hands were on deck as the Bears pulled off a 73-70 win over the nation's No. 20 team. Read more
The wind cuts, the wind gusts, the wind swoops. Read more
Baylor overwhelmed Oklahoma almost from the beginning and took all the suspense out of the Lady Bears' latest Big 12 triumph. Read more
LUBBOCK — After last month's double-digit loss in Waco, Texas Tech already had plenty of motivation to beat Baylor in Saturday's rematch. Read more
Ryan Bertelsman won't soon forget his first college home run. How could he? The cheers are still ringing in his batting helmet. Read more
Fielding such a young softball team, Baylor coach Glenn Moore didn't expect to glide through the early part of the season without a lot of … Read more
Sitting in his dorm room at Carleton College, Freddie Gillespie had a revelation as he watched a Division I basketball game. Read more
Kalani Brown could notch an historic double-double when the top-ranked Lady Bears host Oklahoma at 7 tonight at the Ferrell Center. Read more
Baylor didn't have to bash its way to the pay station. At times, the Bears simply walked there. Read more
With all-Big 12 pitcher Gia Rodoni out for the season following knee surgery, No. 16 Baylor desperately needs some other pitchers to step u… Read more
It's not that they're sick of seeing each other's faces. That's not it at all. Read more
With its one-and-done players, a deluge of transfers and remarkable parity, college basketball is one of the most unpredictable and transit… Read more
The wait is over for the Baylor softball team to test its new artificial surface at Getterman Stadium. Read more
Baylor's Lauren Cox has been named as one of 10 finalists for the Katrina McClain Award, given to the nation's top power forward. Read more
MANHATTAN, Kan. — Top-ranked Baylor showed off its versatility, as guards Chloe Jackson and Juicy Landrum carried much of the scoring load … Read more
Kim Mulkey is a self-described creature of habit. She follows certain routines, born of both design and her Type A personality. Read more
Baylor receiver Pooh Stricklin is retiring from football after dealing with a number of injuries throughout his college career. Read more
After embarrassing Oklahoma by 30 points two weeks ago in Norman, Baylor knew the Sooners would play more inspired basketball in Round 2. Read more
There isn't a more optimistic coach in college basketball than Scott Drew, so he did his best Sunday afternoon to put a positive spin on Ba… Read more
FAYETTEVILLE, Ark. — Baylor's Wil London turned in another fast-paced weekend, as the senior from Waco won the 400-meter dash title and tur… Read more
Kalani Brown grinned as she leaned into the microphone. Read more
When center Tristan Clark went down with a season-ending knee injury a month ago, Baylor coach Scott Drew hoped he could keep the rest of h… Read more
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Kobe P. Hit2Fit
Kobe P. fought his very first (exhibition) boxing match on Saturday May 5 at the Hit2Fit gala fundraiser. His opponent was a National Bronze medalist and a Provincial champion.
The result was a split decision with both boys winning a medal.
This is pretty impressive for his first ever fight!
Photo of Kobe and his coach Peggy Maerz after his win.
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Combined Server Rejuvenation in a Virtualized Data Center
@article{Machida2012CombinedSR, title={Combined Server Rejuvenation in a Virtualized Data Center}, author={Fumio Machida and Jianwen Xiang and Kumiko Tadano and Yoshiharu Maeno}, journal={2012 9th International Conference on Ubiquitous Intelligence and Computing and 9th International Conference on Autonomic and Trusted Computing}, year={2012}, pages={486-493} }
- Published 2012 in 2012 9th International Conference on Ubiquitous…
This paper presents a high-availability solution to a virtualized data center which consists of a cluster of physical servers and hosted virtual machines (VMs). VMs are typically generated through software called virtual machine monitor (VMM) running on physical servers. Both VMs and VMMs face the risks of software aging which are caused by aging-related bugs in the software and may result in failures in long time execution. To prevent such failures proactively, we propose a combined server… CONTINUE READING
Publications citing this paper.
Showing 1-8 of 8 extracted citations
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Xen and the art of virtualization
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Enabling VM Survival Across Hypervisor Failures
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ReHype: enabling VM survival across hypervisor failures
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Great bag. versatile and lots of room if you just want to travel with one piece of luggage. Read more reviews on Feefo
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Toshiba 15.6" Laptop AMD 4GB RAM 500GB HD Webcam & DVD Burner
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Toshiba 15.6" Laptop AMD 4GB RAM 500GB HD Webcam & DVD Burner
Reviews (18)
18 reviews
Cool I guess! Doesn't have much to it but it is decent, as decent as Windows ever will be.. #TeamApple
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Home » Apartments » Choosing Unique Christmas Centerpieces for Table in Dining Room » Stunning Dining Room Ideas With Christmas Table Decoration Feat Christmas Snowman Cake Decorations Also Christmas Trees With Lights Image
When it is Christmas, you usually will be busy to cook special food because you have a plan to invite friends, family and all of people to come to your home. You can celebrate Christmas together and do some activities together too. Chris


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Protecting Your Eyesight
Your eyes are valuable and worth protecting! Failure to follow proper eye protection practices can result in injury, infection, eye disease and even loss of sight.
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Protect your Eyes from Harmful UV Light. When outdoors during the daytime, always wear sunglasses that shield your eyes from 100% of the sun's harmful ultraviolet rays. This may help reduce your risk of cataracts, pinguecula and other eye problems.
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2337 Minolta Court
$350,000, 3 Beds 2 Baths 1,768 Sqft .18 Acres 2 Garages
About This Property
Beautiful 3 Bedroom* Single Story*Large Lot, Fully Fenced Backyard*One Original Owner, Extremely Well Maintained*Nicely Upgraded with Granite Counters, Stainless Kitchen Sink, Artificial Turf in Front and Back*Water Softener*Alarm*Home is Situated in a Quiet Cul-de-Sac, Within Walking Distance to the Community Center and Club House. Ask about any furnishings for sale outside of escrow.
Essential Information
Community Information
Satellite Dish, Underground Utilities, Cable TV Wired
CC&RS, Age Restricted, Clubhouse, COMMUNITY Facilities, COMMUNITY Pool, Exercise Room, Fitness Center, Jogging, Indoor Pool, Recreation Room, Tennis Courts
Attached, Auto Door Opener(s), Entryto House
Interior Features
Alarm System-Owned, Ceiling Fan(s), Shutters, Windows Coverings Throughout
Microwave, Water Conditioner-Owned
Exterior Features
Back Yard Access, Barbeque Stub, Covered Patio
School Information
Additional Information
2337 Minolta Court, 606 - Henderson (MLS® #2038056) Property History
Properties Similar to 2337 Minolta Court, 606 - Henderson, NV
2 Bed • 2 Bath • 1,596 Sf
2498 Starlight Valley Street, Las Vegas
Beautiful 2 Bedrooms 2 Bath Home Has A Huge Master Walk-in Closet, Open Kitchen Leading To An Oversized Family Room And Adjacent Den. Separate Lau...View Listing
3 Bed • 2 Bath • 1,935 Sf
2672 Ornate Regiment Street, Henderson
Beautiful "almost New" Single Story In Gated Community Of Park At Inspirada. Balance Of Builder's Home Warranty Transferable. Open Floor Plan & Va...View Listing
3 Bed • 3 Bath • 1,898 Sf
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Turnkey Home With 2 Car Garage, Beautiful Backyard, Pool, And Incredible Views Of The Mountains. Great Location In Anthem Highlands With Great Sch...View Listing
Property Listed by: Loraine Malstrom of The Right Choice Realty
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West University Place Police Department weekly report
Feb. 8 An Officer was dispatched to the 4100 block of Tennyson to speak with a reportee in regards to a criminal mischief that already occurred. Feb. 9 ...
Bellaire Police Department weekly report
Jan. 28 At 3:36 a.m., Officer Barrientos was dispatched to the 5200 block of IH 610 northbound in reference to an intoxicated driver. Ofc. Barrientos located the vehicle at 6130 ...
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Hermann Park Conservancy held a master plan community meeting on Feb. 7 to discuss the newest plans for the park, called The Commons. The section of the park to be developed into The ...Continue Reading
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Stratford senior Emma Stephenson and Bellaire senior Briggs Shiller were among those donning gold medals to cap their high school swimming careers after exceptional showings at the UIL Class 6A ...Continue Reading
Police: Houston man arrested after offering-up dog for bestiality
A Houston man is behind bars Thursday, accused of offering up his dog for bestiality to an undercover police officer. Three of his dogs believed to be used for bestiality were also seized. The ...Continue Reading
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Tracy Maness, Staff writer
The West University Place City Council voted to add four charter amendments to the May 4 ballot but deferred adding one that would have potentially decreased the city's debt limit. At the Monday, Feb. ...Continue Reading
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Feb. 1 At 10:34 a.m., an officer was dispatched to the 3000 block of Wroxton in reference to a burglary of a motor vehicle that had already occurred. At 12:38 ...Continue Reading
Madison relies on five to extend district championship run in
By Jack Marrion, Staff writer
The Madison girls basketball team completed its fourth consecutive undefeated district championship in thrilling fashion Feb. 5 at Butler Fieldhouse. Senior Yumon Simien knocked down two free throws with ...Continue Reading
Business owners voice concerns as Bellaire council considers
By Tracy Maness, Staff writer
A plan to turn downtown Bellaire into a more pedestrian-friendly urban village is set to go before the city council on Monday, Feb. 18, but area business owners have voiced concerns. The ...Continue Reading
AT&T withdraws rezoning application in West University
By Tracy Maness, Staff writer
AT&T withdrew its application on Thursday, Feb. 7, that asked the city of West University Place to rezone six lots on Ruskin Street. AT&T has owned the residential lots for ...Continue Reading
More Examiner - Bellaire, West University, River Oaks News
New volunteers, clients needed as donation expands J's Senior Companions Program
An inside look at a $5.5M River Oaks mansion tied to some of the city's most prominent figures
Artists Lounge Live and A.D. Players present Get Happy: Angela Ingersoll Sings Judy Garland
3 Texas towns, including Houston's West University, among 'America's 100 Richest Places'
St. Agnes, St. Thomas swim to TAPPS state championships
Long-time Houston doctor heads to prison for tax evasion
Man wanted in string of armed robberies in southwest Houston
St. Thomas soccer on pace to surpass record season
Suspect arrested after allegedly using sledgehammer to bust into company safe at Metro PCS store
Lamar women's lacrosse prepares for six-game home schedule