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Forum For Integrated Development Ngo In Hyderabad Andhra Pradesh
Forum For Integrated Development Ngo Information
FORUM FOR INTEGRATED DEVELOPMENT is located in Hyderabad Andhra Pradesh . FORUM FOR INTEGRATED DEVELOPMENT is registered as a Society at Hyderabad of state Andhra Pradesh with Ngo unique registration ID 'AP/2010/0029020' . The NGO registration is done by Registrar of Societies with registration number 74/95 on the date of 10-03-1995, Its parent ORganaisation is PaulFoundation. The Chairman of FORUM FOR INTEGRATED DEVELOPMENT is Manjula and Chief functionary officer is Dr VVsaudevarao. Promoters are RV Subbarao, PSrinivasa rao, ,.
Forum For Integrated Development Society Organization / Ngo Information
Registration Details
FORUM FOR INTEGRATED DEVELOPMENT Contact details
Key Issues and Operational Areas, Major Activiities & Achivements
This FORUM FOR INTEGRATED DEVELOPMENT NGO charity is working on Key Issues of Agriculture, Micro Small & Medium Enterprises, Rural Development & Poverty Alleviation, Vocational Training, Women's Development & Empowerment
Andhra Pradesh, Maharashtra, Orissa
FORUM FOR INTEGRATED DEVELOPMENT Major Activities and Achivements
JULY 2008 ONWARDS: Chetana Organic & Fair Trade Cotton Supply Chain Programme India : The programme is currently operational in the semi-arid and predominantly tribal areas/regions of Andhra Pradesh (Telangana), Maharashtra (Vidarbha) and Orissa(SW). The programme aims to improve the livelihoods (option) of the resource poor smallholder farmer households engaged in cotton cultivation. FFID provides technical and IB Support. As on date, more than 6,500 farmers are registered with the Chetana programme and benefit from taking up organic agriculture and ecological farming practice and having access to markets 2005 � 2007 Natural Resource Management (NRM) This project has benefited 49 women SHG (692 women members) from the drought prone Mahaboobnagar district of AP with CAPART�s support 2004 Save the Grain Campaign Created awareness among the women self help groups in Kottur mandal of Mahaboobnagar district of AP with Ministry of Consumer Affairs and Dept. of Food & Public Distribution of the Govt. of India (GoI) support. 2003 - 2007 Social Engineering Activities Initiated in 64 villages from the three rainfed districts of Adilabad, Medak and Nizamabad in AP and while covering 19,449 small holder farmers and 46,754 acres and as part of 48 lift irrigation schemes of the Govt. of AP (GoAP).Supported By: Andhra Pradesh State Irrigation Development Corporation (APSIDC), GoAP. 2003-04 FAO-EU IPM Programme For Cotton in Asia Organized 125 farmers from 5 villages of Nawabpet Mandal of MBN district of AP. Initiated and conducted participatory Farmer Field Schools (FFS) on a weekly basis during the cropping season. Supported By: Food & Agriculture Organization of the United Nations (FAO of UN) and European Union (EU)
- CHAITHANYA MAHILA MANDALI in Hyderabad Andhra Pradesh
- A R EDUCATIONAL ACADEMY in Hyderabad Andhra Pradesh
- AADARANA in hyderabad Andhra Pradesh
- AADARANA MAHILA VIKASA KENDRAM in HYDERABAD Andhra Pradesh
- AAKRUTHI in Hyderabad Andhra Pradesh
- AARAMBH ASSOCIATION FOR AUTISM in HYDERABAD Andhra Pradesh
- AAROHAN FOUNDATION in Hyderabad Andhra Pradesh
- AASHA RURAL HEALTH MISSION in Hyderabad Andhra Pradesh
- AATHMEEYA MANASIKA VIKASA KENDRAM in Hyderabad Andhra Pradesh
- ABHAY GAU SEVA SANSTHAN in Hyderabad Andhra Pradesh
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FORUM FOR INTEGRATED DEVELOPMENT [Ngos with same name]
Popular NGO Cities
Popular NGO Categories
- Education and Literacy
- Health and Family Welfare
- Children
- Environment and Forests
- Agriculture
- Art and Culture
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text: Seller: stonekeeper7 (878) 100%, Location: Beaverton, Oregon, Ships to: US & many other countries, Item: 292750356648 20mm x 18mm x 15mm Thick VERY RARE AUTHENTIC NATURAL Tumbled NUUMMITE from GREENLAND. 21.4grams (107ct approx.) my item # 76G The flash is only visible as an iridescence or reflection of light from specific angles. this item has flash on most sides Nuummite (Mg2)(Mg5)Si8=22(OH)2 For those of who don't know what nuummite is, it is a metamorphic rock containing a "series" of two minerals: Anthrophyllite and Gedrite, both varieties of the amphibole family. According to Rodgers et. al (1996) the age is believed to be around 2.68 Billion (+/- .22 GA). Of course the older the rock, the higher percentage that an error occurs when trying to measure these things. Nuummite, hardness 5.5 -6, draws from the fiery energies of ancient Earth and combines with the elements of Storm. This talisman shimmers with mystical gold light. Other common minerals in the rock are pyrite and Chalcopyrite, which form shimmering yellow bands in polished specimens. It is a stone of personal magic, increasing the frequency of synchronicities and luck, clairvoyance and intuition, and for those evolved enough to work with its intensity, it allows for journeying deep into the personal psyche, offering a clear vision of one's true Self. The oldest mineral on Earth, Nuummite was formed over three billion years ago from volcanic origins. It is a unique combination of Anthophyllite and Gedrite metamorphosed into closely inter-grown crystals of charcoal gray to black with iridescent flashes of gold, though its labradorescent display may include red, orange, yellow, green, silver, blue and violet. It is named for the remote, rugged mountain area north of Nuuk, Greenland, where it was discovered in 1982 and is still painstakingly mined today. Similar rock has been discovered in Canada and the United States, however only the rare Greenland variety has color developed well enough to be suitable for gemstones. Nuummite is a stone of tremendous grounding, attuned to the elemental forces of Earth that can be drawn upon in times of need. It is an excellent source of energy with a strong electro-magnetic field. It aligns the subtle bodies, strengthens the auric shield, and is fiercely protective against negative energies, manipulations, and environmental pollutants. Nuummite is not only a source of energy for the body it also assists in reducing tension and stress, and allows for a deep sleep.It is useful in over-all healing and tissue regeneration, and to relieve pain and discomfort associated with headaches and degenerative disease. It may be used to purify the blood and kidneys, and to alleviate infections that are slow to heal or recur. Nuummite may be beneficial in insulin production and regulation, in treating diabetes, low blood sugar and low blood pressure, fainting and dizziness. It has been used to treat tinnitus in the ears, and disorders of the eyes and throat. Nuummite stimulates circulation and strengthens the triple-burner meridian. It may assist in the recovery of Parkinson's disease and in treating disorders of the central nervous system. It is thought to be fortifying for the brain and increasing mental acuity. This energy of this stone is also highly protective, especially against stronger levels of magic and sorcery that may be directed against you. The type locality for nuummite, which most come from is the southwestern portion of Greenland called Nuuk. Rodgers, K.A., P.D. Kinny, V.R. McGregor, G.R. Clark & G.S. Henderson. 1996. Iridescent anthophyllite-gedrite from Simiuttat, Nuuk District, southern West Greenland: composition, exsolution, age. Mineralogical Magazine 60: 937-947. Champness, P.E. & K.A. Rodgers. 2000. The origin of iridescence in anthophyllite-gedrite from Simiuttat, Nuuk District, southern West Greenland. Mineralogical Magazine 64: 885-889. Please visit me at eBay stores- Seven Rays Crystal LLC , Thanks sign up for the store news letter for new listings and sales events. The pictures are of the item you will receive. Follow my new listings on twitter- user name: stonekeeper777 All the crystals, minerals and specimens that I sell are purchased from reliable, trusted companies, some with 28 years of supplying the highest quality products from around the world. I have experimented myself with hundreds of crystals and minerals and know from my experience that they have amazing characteristics that can heal, help you sleep, relax muscles, relieve pain and reduce depression and stress, even lower blood pressure. COMBINED SHIPPING; I will ship your order in a USPS flat rate box and am willing to put as many multiple purchases as I can fit in the box (8.5 inches x 5.5 inches x 1.5 inches) for the same price. If you purchase multiple items, add them to your cart and ask for a total or an invoice, wait until I send you an e-mail with an invoice that combines shipping, then send the payment. If you cannot do it that way, I can refund the extra shipping cost back to you on Pay Pal upon printing the label. I prefer USPS flat rate boxes because the tracking keeps any problems from arising. It comes with free insurance up to $100.00. The same size box sent first class with added insurance usually costs more to ship. INTERNATIONAL SHIPPING; I am in the eBay GLOBAL SHIPPING PROGRAM. This is a third party shipper that readies customs papers and computes the Shipping, Import taxes and Duties for your Country. They forward your package to the correct shipper from the USA to you. Buyers are responsible for all shipping costs including taxes, duties and import fees or any other fees required by your Country. There is NO combined shipping on International orders. PAYMENT; quick payment is appreciated. Disclaimer: The metaphysical and healing properties outlined for my products are for inspiration and reference. These properties are dependent upon the attitude and beliefs of the individual. In no way are they meant to replace diagnosis or treatment by a qualified therapist or physician. My goal is to facilitate crystal healing. Orgone Ancient shamens, seers,wiccans, healers & all other energy practitioners from the beginning of time, have in one way or another tapped into this ocean of unlimited power which surrounds the entire planet, universe & multidimensions. Edger Cacy, Franz Anton Mesmer, Nikola Tesla, Wilhelm Reich, Fra Walter Russell, Viktor Schauberger, Spiritual Gurus, Jesus, Buddha, and the countless other saints avatars, awaken masters, & mundane people. Fascinating as it appears, few have adquately & accurately described or interpreted what this cosmic creative energy, really is. What westerns have come to termed "Orgone" or "Life-Force" energy, is also known as numerous different names by the people of those geographic regions. In Asia & Middle East- as Chi, Ki, Mana, & Prana Energy. In North America & Europe: as Vril, Life-Force, Animal magnetism, Morphogenetic field, Unified Field, Psi, Psychic mind power, Pneuma, Light, Light energy, Odic force, Sekhem, Reiki Prana, Tachyon & many others. All the names are just a interpretation and conceptualization of the indigenous peoples attempt to understand and help others to grasp this basic omnipresent, ever-flowing cosmic energy substance. Sevens Seven is the symbolic number of the universe, or macrocosm, with the human as the microcosm, so it was therefore connected in numerology to the perfect man. It is also the number attributed to Mother Earth, safety, perfection, plenty and fecundity. Figure 2 – A British Fifty Pence piece has seven sides and an image of the universal goddess (Queen Semiramis). Most importantly, seven was deemed important in the process towards the enlightenment experience. Three signified the heavenly part of the seven, and four the earth and human part. Only together would they unite as seven. In other words, one climbed the tree or ladder – the four lower chakras associated with the external material realm – to reach the three organs or glands (power centres) in the head associated with the internal psychical realm. There were seven in all. is the ultimate source of the significance of sevens in myth, legend and mystical and spiritual systems the world over. Here are just a few of the correspondences: Seven chakras ('wheel' vortices), reflecting the seven levels of consciousness and existence. The reality limit of these seven levels is demonstrated in the piece of paper that cannot be folded more than seven times. Seven elementary hues to the spectrum, blending to form white. This has correspondence with the climatic phase of the kundalini awakening, the explosion of 'bright white light' at the centre of the head. Seven notes on a scale. Seven electron orbits, levels or 'shells' of the atom. Seven days in a week. Seven days of Creation. Seven Sacraments of the Catholic Church. Seven Deadly Sins and Seven Virtues. Seven Last Words of Jesus. The gospels record seven 'words' or utterances of Christ from the cross before his 'death' and 'resurrection'. The cross is the shamanic world tree or axis mundi and therefore also the human spinal column. Speaking seven times from the cross/spine symbolizes the seven chakras, and the culmination of all seven is the 'feigning death' experience that leads to the 'resurrection' of enlightenment. Seven Joys and Seven Sorrows of Mary. Joshua walked around Jericho seven times before the walls fell down. (Jericho was the first civilization, according to some, and was home to a large group of shamans.) Seven heavens (Qur'an, Bible, Book of Enoch, shamanic and Druid belief). The 'seventh son of the seventh son' Native Americans determined actions based on its effects 'unto the seventh generation'. Seven steps to heaven – a popular belief, as features on ziggurats, pyramids and other artificial mounds based on the shamanic world mountain or primordial mound of creation. Seven Sages or Wise Men of Greece. Seven Ages of Man. Seven senses, according to the ancients. These are under the influence of the seven planets of classical times. Fire moves, earth brings sense of feeling, water gives speech, air taste, mist sight, flowers hearing and the south wind smell. Seven Wonders of the ancient world. Seven Pillars of Wisdom in Islam. Seven Sages or Shining Ones of ancient Sumer and elsewhere. The seven sages of different cultures symbolized the seven chakra levels, and their final deification as a pantheon of seven refers to the human becoming divine through ascending each level and reaching the source of enlightenment and illumination. The Seven Champions of English legend. Seven Japanese gods of luck. HISTORY Today, we understand that all things in the universe are forms of energy with their own frequency and vibration – including crystals. Nikola Tesla declared this concept as the key to understanding the universe and proved how certain forms of energy can alter the vibrational resonance of other forms of energy. This concept is why healing crystals and stones are still used today to align, heal and alter the vibration of bodily cells, chakras, and the subtle bodies by holistic healers. The ancients didn't have access to the enlightening scientific information that we have today concerning the power of healing crystals. However, people around the world seemed to instinctively be drawn to these lovely gems and have a deeper understanding of their value and meaning in the greater scheme of the universe. Minerals, gems, and crystals have been used for millennia to enhance emotional, physical and spiritual balance. How the ancients knew, we may never know for sure, but these cultures certainly considered stones a major aspect of their existence. Roman Culture: Talismans and amulets of crystal were typical amongst Romans. Most often, they were considered useful in enhancing health, attracting desirable things, and for providing protection in battle. Ancient Egyptians: One of the biggest historical proponents of healing crystals, the Egyptians buried their dead with quartz upon the forehead. This was believed to help guide the departed safely into the afterlife. Pharaohs toted cylinders filled with quartz to balance the Ba and Ka energies of the body. Strongly associated with the Sky Goddess Isis, crushed Lapis Lazuli stones that were most notably worn by ladies of royalty – like Cleopatra – upon the eyes to promote enlightenment and awareness. Dancers donned rubies in their navels to foster their sex appeal. Many wore crystals over the heart to attract love, and placed crystal-laden crowns upon their head to stimulate enlightenment and awaken the third eye. Chinese Culture: Chinese medicine commonly incorporates the use of healing crystals – including crystal-tipped needles used in acupuncture and Pranic healing sessions. These traditions hail from nearly 5000 years of practice. Ancient Greeks: Crushed hematite was often rubbed upon soldier's bodies prior to entering battle with the idea that it made them invincible. Interestingly, the word 'crystal' is thought to derive from the Greek word 'krustullos,' – meaning 'ice' – and until the 1500's many ancients believed stones like clear quartz crystals were eternal ice sent from the Heavens. The mythological story of Amethyst plays a vital role in the story of the God Dionysus and Goddess Diana, and the word is said to be the Greek translation of 'sober' or 'not drunken' – something Dionysus could have spent more time being. Traditions in India: Aryuvedic medicine in India considers crystals valuable for healing emotional and metaphysical imbalances. The use of various healing crystals is documented within the pages of the Hindu Vedas, which also references each stone's specific healing abilities. Sapphires are thought to bring astuteness, clarity and mental balance, and jasper is thought to bring harmony, sexual vitality, and balance base chakras. Ancient Japanese Beliefs: Scrying was a common practice in early Japanese culture, and it is very similar to looking into a crystal ball as we see some psychics do today. Crystal quartz spheres were considered representative of the heart of a dragon and signified their power and wisdom.Concepts of Electromagnetism Concepts of electromagnetism as proven by James Clerk Maxwell and their interdependent nature along with the various breakthroughs in quantum theory have given us scientific evidence for what the ancients always knew. Because everything vibrates at certain frequencies, they have the ability shift and alter the frequencies of other objects or bodies when they occupy the same space. Hence, a crystal – which vibrates at its own frequency oscillations – vibrates within our own energy field through the physical law of resonance and creates a larger vibrational field, affecting the nervous system and transmitting the information to the brain. In essence, these connecting vibrations can harmonize frequencies and stimulate biochemical shifts that affect physical health in a positive, healing way. Thanks to the amazing author Stephanie Lucas for this article. PRECIOUS STONES OF THE BIBLE Throughout history, in every civilization, gemstones have been highly prized and sought after. The Bible makes numerous references to jewels and precious stones. In Exodus 28:21 the Lord ordains precious stones to be used in the making of the breastpiece of the High Priests' garments. Zechariah 9:16 says that the Lord's people will be like jewels in a crown. In Revelation 21:19-20, John names twelve stones that adorn the foundation of the New Jerusalem. The difficulty has been in knowing the exact identity of the stones named. It has only been since the mid-1800's that we have begun to identify stones according to their mineral content. In Bible times stones were identified by color, such as the Hebrew term odem which simply means "red stone." This could have been any number of red stones known at the time (i.e. red jasper, carnelian, garnet). As more information surfaces thanks to archaeological findings and the writings of ancient historians such as Theophrastus (372-287 BC), Elder Pliny (23-79 AD), and Josephus (37-100 AD), we are able to come a bit closer to making some identifications sure. Condition: New, Country/Region of Manufacture: Greenland, Modified Item: No

subset: commoncrawl


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When life blows sometimes the best thing you can be is a dick.
I hate when I'm on the couch after a long day, I put the TV on and then my family starts having a competition of who can be the loudest.
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Cool story bro. Put it in the history books with all the other boring sh*t I don't care about.


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February 2, 2018 In NEWS
Callie's running the London marathon.
Project Centre's Callie Connelly is running the London marathon to raise money for the British Heart foundation.
On 22 April Callie will strap on his running shoes for the longest run of his life. He'll be running the 26.2 miles in aid of the British Heart Foundation who research heart disease, something which has affected his family.
We're really proud of Callie and are pleased to be sponsoring him, good luck!
Callie said: 'I'm so excited (and a bit nervous) to be doing this with my brother, seeing how heart disease has affected my family makes me want to do what I can to help people with it.'
All donations are welcome, please visit Callie's page: www.justgiving.com/fundraising/callie-connelly1
Latest posts by Project Centre
- Watford Bid's Professional Services award sponsored by Project Centre - February 15, 2019
- Project Centre nominated for two British Parking Awards - February 15, 2019
- Soledad Berbel-Roman on existing barriers to adopt new technologies in the UK infrastructure sector - February 15, 2019


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00412 ed StcVes Apr-Jun ronrnewtal Protection 1988 ncv * ncy 00412A vvEPA QUARTERLY PROGRESS REPORT APRIL-JUNE 1988 Environmental Research Laboratory Duluth, Minnesota 55804
------- UNITED STATES ENVIRONMENTAL PROTECTION AGENCY ENVIRONMENTAL RESEARCH LABORATORY - DULUTH \ PRO^0 6201 CONGDON BOULEVARD DULUTH, MINNESOTA 55804 August 19, 1988 MEMORANDUM SUBJECT: Third Quarter FY88 Progress Report on Projects of the Environmental Research Laboratory-Duluth (ERL-D) FRCM: Nelson A. Thomas, Chair Strategic Oonnunication Council, ERL-Duluth TO: Addressees Attached is a copy of the ERL-Duluth progress report on research projects for the third quarter. The following are highlights from various projects/activites . 0 Research was undertaken to identify and quantify toxic components of sediment in the lower Fox River, WI. Screening tests identified 5 of 12 sediment samples as acutely toxic to ^phnj?i and fish. Project 35, page 1. The National Effluent Toxicity Assessment Center (NETAC) was established at Duluth. Each region was requested to submit one candidate effluent for a TIE evaluation. NETAC will be working closely with all EPA regions and the states to test effluents and to exchange information through workshops and seminars. Project 5, page 3. An ERL-D project on the Fox River/Green Bay watershed was funded by Region V (Great Lakes National Program Office) to focus on sediment criteria and inplace pollutants. Project 2, page 8. Protocols for fractionation, identification and evaluation of toxicity in effluents are scheduled to be published soon. These protocols were successfully used to identify and fractionate components causing toxicity in a number of effluents. Project 37, page 18. The Ecological Risk Assessment Program has undertaken research on the dynamic response of aquatic communities to various contaminants and perturbations. The initial research indicates that most systems are relatively resilient because recovery times were less than 3 years. Exceptions are noted under Project 31, page 32. Attachment: As stated above
------- Table of Contents HIGHLIGHTS Water Quality Duluth Aquatic Life Sediment Criteria Development WQ Toxicity-Based NPCES Permits Methods WQ Assessment Techniques Ecological Research with the Peoples* Republic of China Aquatic Life WQ Criteria Develcpment/Modifications Grosse lie Sources-Fate-Effects of Toxic Substances in Great Takes Mass Balance Models for Toxics in Freshwater Systems Tech Assistance for GLNFO, ow, LJC, state & Local Gov. Hazardous Waste Leachate Toxicity Profiles for HW Characterization Predicting Aquatic Toxicity of HW Constituents & Exposures Pesticides Field Validation for Hazard Assessment Techniques Develop Guideline Protocols & Test for BCA Effects Develop Methods for Predicting Susceptible Populations Chemical Testing & Assessment Aquatic Toxicology Structure-Activity Relationships & Estimation Techniques Predicting Ecosystem Resilience Multi Media Energy Watershed Manipulation Project Project Officer Page A. Carlson N. Thomas A. Carlson N. Thomas A. Carlson R. Kreis W. Richardson W. Richardson G. R. Nieml Erickson R. Siefert R. Anderson F. Stay S. Broderius G. Veith G. Niemi J. Eaton 1 3 6 8 10 13 15 17 18 20 22 24 28 28 30 32 34
------- PRINT DATE: 08/16/88 PMS-060 CTJFNT OFFICE AA OW THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of EnvirOTunental Processes and Effects Research ERL DLTH: APRIL - JUNE 1988 **************************** CODE TTTIE BUDGET SUB-ACTIVITY: ISSUE: PPA (L) : B101 WATER QUALITY A WQBA/i*WlLTriNG 09 AQUATIC LIFE SEDIMENT CRITERIA DEVELOPMENT RC: N ERL DLTH PROJECT: 35 Aquatic Life Sediment Criteria Development PROJECT OFFICER: Anthony R. Carlson PHONE: -780-5523 ****************************************** PLANNED START: 10/01/87 PLANNED END: 12/31/99 ****************************************** PROJECT DESCRIPTION; GOAL: Develop sediment quality criteria protocols for use in protecting aquatic life. RATIONALE: Sediment quality criteria can be used to form the basis for state sediment quality standards, NPDES permits and assessment of in-place sediment contaminants. APPROACH: Determine relationships between tissue residue and just barely safe toxic endpoints for sediment- associated organisms chronically exposed to specific non-polar organic chemicals and metals. Calculate criteria based on just barely safe residue concentrations. Evaluate and validate under field conditions. PROJECT STATUS AND ACCOMPLISHMENTS TO DATE: Work plans are being written. Data is being compiled from the literature for use in determining the relative sensitivity of benthic and non-benthic organisms to specific chemicals. A research project was undertaken to identify and quantify toxic components of the lower Fox River, Wisconsin sediments. Preliminary toxicity screening tests identified 5 of 12 sediment samples as being acutely toxic to Daphnia and fish. Definitive studies are planned for this summer and fall.
------- 7896 DUE: 08/31/89 REVISED: COMPLETED: RETORT ON DEVELOPMENT OF TEST METHODS WITH BENTHIC ORGANISMS TO DEFINE THE BIQAVAILABILITY AND/OR SEDIMENT-BOUND TOXICANTS. 7969 DUE: 05/31/89 REVISED: COMPLETED: REPORT ON MINIMUM TOX. DATA SET FOR SED.QUAL.CRITERIA BASED ON RELATIVE SENSITIVITY OF BENTHIC AND NON-BENTHIC ORGANISMS 7971 DUE: 05/31/89 REVISED: COMPLETED: REPORT ON BIOLOGICAL ASSESSMENT OF KNOWN SEDIMENT-ACTIVITY OF METAL IONS IN PORE WATER AND ITS TOXIdTY 7973 DUE: 05/31/89 REVISED: COMPLETED: REPORT ON FIELD VERIFICATION STUDIES OF LABORATORY OBSERVATIONS WITH FIELD MEASUREMENTS USING BENTHIC DATA OF METAL
------- PRINT DATE: 08/16/88 CLIENT OFFICE OWEP PMS-060 THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DLTH: APRIL - JUNE 1988 ****************************************** CODE TITLE BUDGET SUB-ACTIVITY: B101 WATER QUALITY ISSUE: A WQEA/PERMTTTING PPA (L): 11 WQ TOXICnY-BASED NFDES PERMIT METHODS RC: N ERL DLTH PROJECT: 05 WQ Toxicity-Based NPDES Permits Methods PROJECT OFFICER: Nelson A. Thomas PHONE: -780-5702 ****************************************** PLANNED START: 10/01/85 PLANNED END: 12/01/91 ****************************************** PROJECT DESCRIPTION; GOAL: Develop the scientific basis for the incorporation of the toxicity approach into the control of toxics from effluents. RATIONALE: There is a need to control toxics in effluents when chemical specific criteria are not available and/or the toxicant is not known. The major need is the new emphasis on the use of Water Quality Criteria in the next round of NPDES industrial permits. There is a need to identify and evaluate the causes of toxicity in toxic effluents. APPROACH: Short-term chronic toxicity tests have been and will continue to be developed for testing municipal and industrial effluents and receiving waters. The tests have been positively evaluated as to their ability to predict ecosystem impact through a series of field studies. A protocol for conducting the effluent and ambient tests will be prepared and tested. As validation has been established, issues related to persistence, bioaccumulation, additivity of multiple discharges, fractionation/separation and permit development under an integrated approach will be the focus of the research. Increased emphasis will be placed on the evaluation and identification of a chemical causing the toxicity. A protocol for assessing bioaccumulation is being developed and will be field tested. PROJECT STATUS AND ACCCMPLISHMENTS TO DATE; Evaluations are complete on the 7-day chronic Ceriodaphnia and fathead minnow toxicity tests to predict instream impact at nine sites. ERL-D provided the support for the toxicity testing, while field studies were supported by the Office of Water, Permits Division and ERL-D. Site reports are complete and the revised statistical methods to analyze the combined effects of mortality and production of young per female for the Ceriodaphnia and weight for the fathead minnows were developed. Methods were reviewed to assemble an approach to address the persistence of toxicity.
------- Technical assistance on methods continues. Ceriodaphnia cultures are being sent to states, EPA regions, contract laboratories, universities and industry. Emphasis is being placed on toxicity testing to aid in toxicity reduction evaluations. ERL-D is combining toxicity testing with chemical fractionation, called toxicity identification evaluations (TIEs). This permits more positive coupling of chemical identifications with toxicity. Acute toxicity tests with Ceriodaphnia, Daphnia magna, medaka and fathead minnows are used to assess toxicity of whole and effluent fractions. A TIE indicated that a treated effluent owed its toxicity to 600 ug/1 nickel, nearly 8 times the lethal limit for aquatic organisms. Region 9 requested an extensive evaluation of the las Vegas POTW. The TIE determined that diazinon and dichlorvos were the primary cause of toxicity and 5 effluents were partially identified. Overall 46 effluents were evaluated at least once as potential candidates for the TIE work. A report on the Hollywood POTW TIE was sent to Region 4. Toxicants responsible for the toxicity were diazinon and chlorfenvinphos. Also, 4 effluents are being tested and characterized for cause of toxicity. Region 5 sent a sample to determine whether something other than ammonia is causing the toxicity. This effluent was characterized and it was determined that ammonia did not cause the toxicity. The announcement of the National Effluent Toxicity Assessment Center (NETAC) was made in March. Each region was requested to submit one candidate effluent for a TIE evaluation. Candidate effluents must have acute toxicity. Seven of the 10 regions have formally submitted candidate effluents from which 2 effluents were chosen. Also, 1 effluent has been received and another is scheduled to arrive. A TIE techniques workshop was presented by Don MDunt to 30 attendees from Region 5 state agencies. Similar workshops are planned for Atlanta, GA and South Carolina. A toxicity methods workshop will be conducted in Aug/Sept. for Region 9 (California, Arizona). A July methods data interpretation and site study workshop is planned for Region 6. A new document on Phase I TIE procedures is available. An extensive field project involving ERL-D, ERL-N and EPA-Newport working with the California Regional Water Quality Board to assess the toxicity and impact of selected discharges into the San Francisco Bay was conducted. The effort consisted of TIE work and a comparison of the fresh and saltwater methods. The report, which is complete and in inhouse review, is available in draft form. An October field trip to Casper, WY was requested by Region 8. TIE and toxicity tests were conducted to demonstrate to the Region the feasibility of conducting the procedures and to determine whether toxicity in the mining operations was due to more than salt generated during the mining process. Reports on both sites are now available. Based on the analysis of POTW's in our TIE work, diazinon has appeared in several effluents. A survey is in progress of 20-25 POTW plants in the 10 EPA regions to determine whether the occurrence of diazinon is widespread. Samples have arrived from 6 regions. A chemical analysis procedure is being developed and tested to identify bioconcentratable materials in effluents. A contract to collect effluents and place clams in streams to evaluate the bioaccumulation potential of an effluent is complete. Samples are being extracted and analyzed. A guideline on this approach is available.
------- Cooperative agreements are in place to evaluate 4-d vs 7-d Ceriodaphnia tests, to develop techniques to generate ephippial egg production and hatching and to develop standard freeze-dried foods for use in culturing Ceriodaphnia. CETIS: Funding is not available and all tasks are on hold. TTUS AND SQranrrR OF 7163 DUE: 12/31/87 REVISED: 04/30/89 COMPLETED: MANUSCRIPT ON TOXKTTY IDENTIFICATION IN EFFLUENTS 7814 DUE: 06/30/88 REVISED: COMPLETED: REPORT ON THE FIELD AND LABORATORY TESTING OF THE BIOCCNCENTRATICN FACTOR (BCF) PROTOCOL USING FRESHWATER ORGANISMS 7815 DUE: 05/31/88 REVISED: COMPLETED: REPORT ON THE TOXICTIY IDENTIFICATION OF THE TOXIC COMPONENT (S) OF EFFLUENTS 7816 DUE: 06/30/88 REVISED: COMPLETED: 05/31/88 REVISED TOXnCTTY IDENTIFICATION PROTOCOL TO IDENTIFY THE CAUSES OF TOXECTTY IN EFFLUENTS 7823 DUE: 09/30/89 REVISED: COMPLETED: PROTOCOL FOR A FOUR-DAY CERIODAPHNIA DUBIA TEST METHOD 7824 DUE: 09/30/89 REVISED: COMPLETED: REPORT ON THE COMPARATIVE SENSITIVITY OF A FOUR-DAY TO SEVEN DAY CERIODAPHNIA DUBIA TEST TO SINGLE TOXICANTS
------- PRINT DATE: 08/16/88 CLIENT OFFICE AA OW PMS-060 THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DUTH: APRIL - JUNE 1988 ************************************* CODE TITLE BUDGET SUB-ACTIVITY : B101 WATER QUALITY ISSUE: A PPA (L) : 12 INTEGRATED WATER QUALITY ASSESSMENT TECHNIQUES RC: N ERL DLTH PROJECT: 01 Water Quality Assessment Techniques PROJECT OFFICER: Anthony R. Carlson PHONE: -780-5523 ****************************************** PLANNED START: 10/01/85 PLANNED END: 09/01/88 ****************************************** PROJECT DESCRIPTION: GOAL: Develop, evaluate and/or demonstrate the validity of toxicological concepts for deriving numerical water quality criteria for use in protecting aquatic life from point and non-point pollutants. RATIONALE: Scientifically defensible methodologies for use in protecting aquatic life and its uses are needed by program offices. APPROACH: Evaluate water quality criteria protectiveness and new criteria derivation methodologies to assess their validity under semi-natural conditions of outdoor experimental streams located at the Monticello Ecological Research Station and in real world situations. Apply water quality criteria, effluent toxicity and effluent toxicant identification methodologies to non-point source pollutant control. Conceptualize and study fluctuating exposure-dose response relationships and apply results to water quality criteria derivation. Develop concepts and guidelines for toxicity factors in criteria applications. Develop a basic approach of coupling non-point pollution loadings and aquatic life impacts with the goals of identifying remedial benefits of BMP" s. PROJECT STATUS AND ACCOMPT-TSHMKKns TO DATE: A proposal for a one-year ecological risk demonstration was forwarded to DEFER, and provides for a joint project by ERL-Duluth (toxicity prediction) and ERL-A (exposure assessment) . Exposure analyses will involve documentation of internal and external heavy metal sources to the upper Clark Fork River, both under normal ranges of river conditions and extreme flow events. Toxicity assessment will estimate fish mortalities due to the unsteady state exposures, joint toxicity, and effects of associated physical and chemical factors.
------- Reviews were completed on a proposed contract between the the Montana Department of Health Environmental Services and the University of Montana for a periphyton growth and nutrient loading study for the Clark Fork River/Lake Pend oreille system. Region VIII awarded the study funds. Ambient toxicity tests continue on samples collected from the upper Illinois River basin. At several stations instream toxicity was noted to Ceriodaphnia and fathead minnows. Tests were also conducted with the green alga, Selenastrum capricornutum, to biologically characterize the water's nutrient status. Both growth stimulation and inhibition effects were found. Additional testing was completed this spring and summer. An ERL-D project on the Fox River/Green Bay watershed was funded by Region V (Great lakes National Program Office) . The project focuses on sediment criteria and inplace pollutants. Sediment criteria will involve developing methods to determine "safe" conditions using a toxicity/residue approach. Inplace pollutants will generate protocols to evaluate sediment quality and physical/chemical modeling. S AND SCPEmTB Qp 7170 DUE: 09/30/87 REVISED; 09/30/89 COMPLETED: KEPT. GN THE FEAS. OF PEED. THE taftHKiS AND INCORP. FLUCTUATING EXP. IN THE APPLICATION OF WQC AND EFFLUENT TOXICS TESTS 7187 DUE: 03/31/88 REVISED: COMPLETED: 03/31/88 REPORT GN IMPACTS OF AMGNIA/CHLORINE ON ECOSYSTEM STRUCTURE AND FUNCTION IN EXPERIMENTAL STREAMS 7827 DUE: 04/30/89 REVISED: COMPLETED: _ PROGRESS REPORT ON THE INFLUENCE OF SELENIUM IV ON BLUEGTLL REPRODUCTION IN OUTDOOR EXPERIMENTAL STREAMS 7830 DUE: 10/31/90 REVISED: COMPLETED: REPORT ON APPLICABILITY OF WATER QUALITY CRITERIA AND ENVIRONMENTAL GOALS - GREEN BAY, WISCONSIN 7831 DUE: 10/31/88 REVISED: COMPLETED: REPORT ON FEASIBILITY OF MERGING OF USGS AND EPA DATA BASES FOR USE IN REGIONAL WATER QUALITY ASSESSMENT 8086 DUE: 12/31/89 REVISED: COMPLETED: PROGRESS REPORT ON NUTRIENT LOADS, ATTACHED ALGAL, AND ENVIRONMENTAL GOALS IN CLARK FORK RIVER AND LAKE PEND OREILLE
------- PRINT DATE: 08/16/88 CLIENT OFFICE AA OW IMS-060 THIRD QUARTER STATUS REPORT ON FY'SS PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DLTH: APRIL - JUNE 1988 ****************************************** POPE; TITLE BUDGET SUB-ACT1 Vl'lY : B101 WATER QUALITY ISSUE: A PPA (L) : 13 ECOLOGICAL RESEARCH WITH THE PEOPLE'S REPUBLIC OF CHINA PC: N ERL DOTH PROJECT: 02 Ecological Research with the People's Republic of China PROJECT OFFICER: Nelson A. Thomas PHONE: -780-5702 ****************************************** PLANNED START: 10/01/84 PLANNED END: 09/01/89 ****************************************** PROJECT DESCRIPTION: GOAL: To participate jointly with the People's Republic of China (PRC) in mutually beneficial studies through a cooperative research proqidin. Scientists from both countries will participate in research and exchange scientific information on the environmental processes and effects of pollution on freshwater organisms. RATIONALE: In support of the United States' policy to provide scientific and technological cooperation with China, the USEPA and PRC in 1980 entered into an agreement known as the US-EEC Environmental Protection Protocol. This agreement provides for establishment of a cooperative research program. APPROACH: Participating scientists from both countries will discuss and identify the specific projects that will be conducted in the research program. Projects (subject to modification and approval) include emphasis on toxicity tests methods, effect of environmental variables on toxicity and toxicity mixtures. Scientists from PRC will study at ERL-D to develop an understanding of the testing of single chemicals and complex effluents. PROJECT STATUS AND ACOOMPT-TSHMrairs TO DATE: The four visiting scientists completed their studies and returned to China. Wang Shida and Zhuang Dehui studied fish and invertebrate test procedures while here and are transferring these procedures to the Wuhan Laboratory. The second group is conducting tests with the mini diluter constructed at ERL-D. Field studies were undertaken in the U.S. and PRC to conduct toxicity tests on effluents and heavy metals. Two experts from ERL-D traveled to Wuhan to begin the joint testing with Chinese species of aquatic life. Toxicity testing and culture procedures will be the focus of the exchanges in the PRC. Field evaluation of the effluent protocol was tested at a steel mill in Wuhan. Two toxicologists from ERL-D traveled to Wuhan to cotplete cooperative testing using cadmium and effluent samples. Reports are being prepared on heavy metal 8
------- toxicity and effluent testing. A work plan is being developed for the histopathological study of exposed fish for teratogenic and carcinogenic effects. A scientist from the Wuhan Laboratory arrived in May for a 12-month exchange of histopathologic methods. Field studies are currently being undertaken for the collection of fish with contaminated sediments. US AND SGHETITTF OF 7832 DUE: 04/30/89 REVISED: OCMPIEIED: REPORT ON THE APPLICATION OF U.S. TEST METHODS FOR WQC DEVELOPMENT, EFFLUENT TOXICHY. TESTING, & AMBIENT TOXLCTIY ASSESSMENT IN THE PRC
------- PRINT DATE: 08/16/88 CLIENT OFFICE AA OW PMS-060 THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DLTH: APRIL - JUNE 1988 BUDGET SUB-ACTIVITY: B101 WATER QUALITY ISSUE: A WQBA/PEBMrnTNG PPA (L): 14 AQUATIC LIFE WQ CRITERIA DEVEK>PMENT/1*X>IFICATION RC: N ERL DLTH PROJECT: 03 Aquatic Life WQ Criteria Development/Modifications PROJECT OFFICER: Anthony R. Carlson PHONE: -780-5523 ****************************************** PLANNED START: 10/01/84 PLANNED END: 09/01/88 ****************************************** PROJECT DESCRIPTION; GOAL: Formulate guidelines for the development of aquatic life water quality criteria and advisories. Prepare aquatic life water quality criteria and advisories. Test criteria under site-specific conditions as to aquatic life protection afforded. RATIONALE: Many states are using the site-specific modification protocol, which requires additional testing of its application. With the need to develop additional water quality, testing of the minimum data set requirements is necessary. Field validation of present and new criteria is required. APPROACH: Laboratory and field studies will be undertaken for the development and validation of the guidelines and criteria. Chronic testing and evaluation of more sensitive endpoints will be undertaken for compounds for which criteria are to be developed. The protection of ecosystem functions will be determined. Issuing aquatic life advisories with minimum data sets will be assessed. The expression of concentration, duration and frequency in the new aquatic life criteria requires the development of methodologies to classify and assess impact on ecosystem as well as to predict recovery. PROJECT STATUS AND ACOCMPT-T.qHMRMTS TO DATE: Six criteria documents (aniline, 2,4-dimethylphenol, phenol, methyl parathion, thallium and tributyltin) are in preparation. A computer data base containing historical fish and water temperature records was compiled for over 300 species of freshwater fishes at 574 stream stations in the United States. A statistical summary of the cumulative frequency of occurrence of stream temperature (i.e., over stations and years) where 30 freshwater fish species were present has been compiled. Temperature envelopes described for these species are collated with temperature requirements determined in laboratory experiments. Adaptations to the seasonal temperature 10
------- cycle are described in full detail for eight species of freshwater fishes as representatives of distinct thermal guilds. The highest weekly mean temperature in U.S. streams for 30 species is evaluated in relation to the ultimate upper incipient lethal temperature, upper zero net growth, physiological optimum and upper spawning threshold for each species. Composite annual temperature regimens were developed for cold-, cool-, and warm-water fishes. Envelopes for thermal guilds can be used to describe the temperature environment for most temperate climate species when data for temperature requirements for individual species are lacking. Ihe relationship between temperature criteria and thermal responses is discussed in relation to maximum weekly mean temperature of streams in summer. Implications for development of thermal criteria are discussed. Acute and chronic toxicity tests using several species of aquatic organisms and acrolein, diazinon and 2,4-D were completed to provide data for water quality criteria development. Toxicity tests with fluoranthene and different aquatic species are presently being planned. The AQUIRE data base has over 101,000 tests on computer file. AQUIRE contains acute, sublethal and BCF toxicity literature for aquatic organisms on 4,900 chemicals and 2,300 organisms, extracted from over 5,500 publications. A series of 30-day early life stage toxicity tests on the effect of fluctuating concentrations of selected organic chemicals on fathead minnows are underway. The streams at Monticello have been exposed to selenium since February 1987. Two streams have been dosed at 30 ug/1, two at 10 ug/1, and two are serving as unexposed controls. Results of field studies have suggested that these concentrations of selenium in the water are not directly responsible for impacts found on fish in aquatic systems. The major route of exposure is thought to be through the consumption of food that has accumulated selenium. In general, fish food organisms in the Monticello streams have attained selenium concentrations proportional to the amount in the water. Because accumulation in fishes to levels that might be harmful can take several months, bluegill sunfish were placed in the streams during September 1987. After eight months of exposure and before spawning, a population count of bluegills was made in the streams exposed to 30 ug/1. This was an unexpected result because laboratory data predicted that this would be a safe concentration. Effects on reproductive success will be evaluated when spawning begins in June. In addition, the effects of selenium on duck reproduction and iirrauno-responses are being evaluated in the streams. Ducks have been in the streams since late April and many pairs have active nests. A journal article on the life-cycle chronic toxicity of fathead minnows exposed to Hf ions, low Ca and elevated Al has been submitted for journal publication. Initial exposures of embryos and larvae of yellow perch, largemouth bass and rock bass have been completed and are described in a submitted journal article. Additional lab tests and in-situ field exposures of rock bass, yellow perch, largemouth bass and black crappies are nearing completion. 11
------- A review of the Appendix D of the Technical Support Document for Water Quality Based Toxics Control indicated the need for additional research and analysis of the literature to better understand the impact of frequency and duration of exposure. Literature review has resulted in the compilation of over 100 case studies in which some aspect of recovery from disturbance was investigated. An in-house report and potential journal article is being prepared. This report will relate case studies to relevant ecological theory and discuss needs for additional research. A recovery workshop is planned for October. The states of Montana, Washington and Idaho have received 300K for FY88 funding for the Clark Fork River/Lake Bend Oreille studies. Details of the specific projects will be discussed during a meeting at Coeur d1 Alene, Idaho on June 29. The discussions will stress experimental design, sampling details and remote sensing. STATUS AND SCPTCTtTTK OF 6525 DUE: 09/30/85 REVISED: 09/30/88 COMPLETED: REPORT EVALUATING THE NEED TO REVISE NATIONAL GUIDELINES FOR DERIVING WATER QUALITY CRITERIA (WQC) 6958 DUE: 06/30/85 REVISED: 09/30/88 COMPLETED: REPORT ON THE NEED AND FEASIBILITY OF REVISING THE OTHER AQUATIC LIFE CRITERIA 6964 DUE: 09/30/87 REVISED: 11/30/89 COMPLETED: REPORT ON ANALYSIS OF FREQUENCY OF CRITERIA EXCEEDANCES AS RELATED TO AQUATIC COMMUNITY IMPACT 7088 DUE: 04/30/87 REVISED: 01/31/88 COMPLETED: 01/31/88 REPORT ON TOXICITY OF METALS AND HYDROGEN IONS IN LOW ALKAUOTTY/HARDNESS WATER 7171 DUE: 12/31/90 REVISED: COMPLETED: REPORT ON FIELD VALIDATION OF METHODS FOR PREDICTING AND ASSESSING FLUCTUATING EXPOSURE 7838 DUE: 09/30/88 REVISED: COMPLETED: FINAL AQUATIC LIFE CRITERIA DOCUMENTS FOR 11 COMPOUNDS 7843 DUE: 09/30/88 REVISED: COMPLETED: DRAFT AQUATIC LIFE CRITERIA DOCUMENTS FOR UP TO 10 COMPOUNDS TO BE SELECTED AND PREPARED JOINTLY WITH THE OFFICE OF WATER 7844 DUE: 09/30/88 REVISED: COMPLETED: DRAFT AQUATIC LIFE ADVISORIES FOR UP TO 45 COMPOUNDS TO BE SELECTED AND PREPARED JOINTLY WITH THE OFFICE OF WATER 12
------- PRINT DATE: 08/16/88 CLIENT OFFICE GLNPO PMS-060 THIRD QUARTER STATUS REPORT ON FY'88 FROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DLTH: APRIL - JUNE 1988 ****************************************** BUDGET SUB-ACTIVITY: B101 WATER QUALITY ISSUE: B MARINE, ESTUARIES & LAKES PPA (L) : 26 SOURCES-FATE-EFFECTS OF TOXIC SUBSTANCES IN GREAT LAKES RC: N ERL DLTH PROJECT: 29 Sources-Fate-Effects of Toxic Substances in Great Takes PROJECT OFFICER: Russell G. Kreis PHONE: 313-675-7706 ****************************************** PLANNED START: 06/01/85 PLANNED END: 12/31/95 ****************************************** PROJECT GOAL: Develop methods to predict effects of inplace pollutants, identify and prioritize sites for remedial action, determine the optimal combination of mitigative strategies, and simulate the results/consequences of actions. RATIONALE: The contaminated sediment problem impacts both freshwater and marine ecosystems; inplace pollutants is a priority research topic in the Great Lakes. The problem of inplace pollutants is long-term even if zero discharge is assumed. Regulatory offices require guidance to establish a cost-effective mitigation policy. APPROACH: An interdisciplinary approach will be used to develop and verify methods to simulate the effects of inplace pollutants and identify and prioritize remedial strategies. Research consists of: 1) field collection, 2) field experimentation, 3) laboratory experimentation, 4) data base development, 5) model development and 6) remedial action guidance. The test sites include impacted "Areas of Concern": Detroit River (1985-1988), lower Fox River - inner Green Bay complex (1987-1992) , and Lake Ontario (1990-1995) . Methods and strategies developed can be used in any "Area of Concern" or other national waterway and may relate to sediment criteria development, implementation of the Clean Water Act and the US/Canada agreements. PROJECT STATUS AND AOOOMPLISHMENTS TO DATE; The In-Place Pollutant Project (IPP) is being conducted in the Trenton Channel of the Detroit River as a component of the Upper Great Takes Connecting Channels Study (UGLCCS) . The project is multidisciplinary and encompasses toxicity tests, measurements of heavy metals and organic contaminants, sediment resuspension, transport and deposition dynamics, vertical toxicity and contamination of sediment, fish tumor surveillance and mathematical modeling. All field work and analytical chemistry, with the exception of small data set, has been completed. An interim report was submitted to UGLCCS and a final 13
------- UGLCCS report will be submitted during the next quarter. Statistical analyses are underway, journal articles were submitted and all tasks are nearing completion. Draft and final project reports are due during the next quarter. A research strategy for contaminated sediments has been developed for the lower Fox River to meet the requirements of three initiations: 1) Assessment and Remedial Strategies for Contaminated Sediments, 2) Sediment Criteria, and 3) Green Bay-lower Fox River Mass Balance. Field studies have begun and will continue into the next quarter. Project on target. STATUS AND SCHETTTTE OF 7204 DUE: 05/31/88 REVISED: 12/31/88 COMPLETED: REPORT ON METHODS FOR PREDICTING PROBABILITY DISTRIBUTION OF EXPOSURE FOR TOXIC SUBSTANCES IN FRESHWATER ECOSYSTEMS 7205 DUE: 09/30/88 REVISED: 12/31/88 COMPLETED: REPORT ON METHODS FOR PREDICTING BIOLOGICAL IMPACTS OF IN-PLACE POLLUTANTS IN THE UPPER G.L. CONNECTING CHANNELS 7877 DUE: 06/30/91 REVISED: COMPLETED: REPORT ON IMPACTS OF RESUSPENSION AND DIFFUSION OF CONTAMINANTS IN GREEN BAY/FOX RIVER (TENTATIVE) 14
------- PRINT DATE: 08/16/88 CLIENT OFFICE GINPO FMS-060 THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DLTH: APRIL - JUNE 1988 ****************************************** CODE TITLE BUDGET SUB-ACTIVITY: B101 WATER QUALITY ISSUE: B MARINE, ESTUARIES & LAKES PPA (L): 26 SCURCES-FATE-EFFECTS OF TOXIC SUBSTANCES IN GREAT IAKES RC: N ERL DLTH PROJECT: 33 Mass Balance Models for Toxics in Freshwater Systems PROJECT OFFICER: William Richardson PHONE: 313-675-7704 ****************************************** PLANNED START: 06/01/85 PLANNED END: 09/30/95 ****************************************** PROJECT DESCRIPTION: GOAL: Conduct mass balance research to link identified ecosystems effects with their causes, assuring that results are related to possible remedial actions. RATIONALE: Over 833 chemical compounds have been identified in Great Lakes ecosystems and biological effects continue to be documented. Fish contamination in many areas has resulted in health advisories and closing of commercial fishing. Site specific mass balance research is required to quantitate the processes and flux of contaminants to predict consequences of remedial actions. APPROACH: Mathematical models for toxic substances based on mass balances, including transport, fate and bioaccumulation processes will be developed, calibrated and verified for important freshwater systems. The research includes: 1) development and application of sampling and analytical chemistry methods appropriate for low level c»ntaminants, 2) development and maintenance of data bases, 3) development and application of mathematical models and other computational techniques. Application will be made in important freshwater systems. Models will be used in other areas as requested and as resources allow. PROJECT STATUS AND ACOOMPT.TSHMfynS TO DATE: Two papers were presented at the IAGLR Annual Conference in Hamilton, Ontario, concerning the results of the Detroit River Mass Balance research. The draft UGLCC mass balance report was received from GINPO and comments were provided to the UGLCC Modeling Committee Chairperson. A workshop was held at LLRS on the Green Bay Mass Balance Project to finalize the modeling and field plans. The primary extramural cooperative agreement package was completed and funding for the University of Notre Dame (Victor Bierman, P.I.) should begin on July 1. Other funding actions were taken for cooperative work with the ERL-Athens and ERL-^arragansett. 15
------- PRINT DATE: 08/16/88 PMS-060 CLIENT OFFICE GLNPO THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DIHH: APRIL - JUNE 1988 ****************************************** CODE TIT' f- BUDGET SUB-ACTIVITY: B101 WATER QUALITY ISSUE: B MARINE, ESTUARIES & LAKES PPA (L) : 26 SOURCES-FATE-EFFECTS OF TOXIC SUBSTANCES IN GREAT IAKES RC: N ERL DLTH PROJECT: 34 Tech Assist, for GLNP, OW, IJC, Regions, States & Local Gov. PROJECT OFFICER: William Richardson PHONE: 313-675-7704 ****************************************** PLANNED START: 01/01/71 PLANNED END: 01/01/99 ****************************************** PROJECT DESCRIPTION; GOAL: To assure Great Lakes research is applied to the regulatory process and to the needs of the Great lakes Water Quality Agreement. RATIONALE: EPA has a primary role in fulfilling the requirements of the 1978 Water Quality Agreement with Canada. The ORD Great Lakes Program at the Large Talc**? Research Station is the primary focus for EPA's response. ORD/LLRS staff and on-site contractors have the experience and knowledge to efficiently fulfill technical assistance requests from GLNPO, IJC, Regions, Office of Water, and state and local governments. APPROACH: Technical assistance will be provided on a priority basis to EPA program offices, IJC, regions, states, and local governments. Specific areas of support will include: 1) maintenance, documentation, application and training for mathematical models, 2) computer service support for water quality and point source data bases, 3) participation on IJC committees and boards, state and local government committees, and 4) providing information to the regulatory community including consultants working for government agencies. PROJECT STATUS AND ACCOMPT-T-c;HMT::^TS TO DATE; The LIBS staff continued to participate on committees for the Upper Great Connecting Channels and the Green Bay studies and to provide Region II Superfund with modeling support for the Hyde Park case. STATUS AND SCHEDULE OF DRT.TVFRARTFS; 8085 DUE: 12/31/88 REVISED: COMPLETED: PILOT CONFINED DISPOSAL FACILITY BIOM3NTTORING FIELD AND DATA REPORT 17
------- PRINT DATE: 08/16/88 CLIENT OFFICE QSW PMS-060 THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DLTH: APRIL - JUNE 1988 BUDGET SUB-ACTIVITY: D109 HAZARDOUS WASTE ISSUE: C WASTE CHARACTERIZATION PPA (L) : 96 WASTE FUTURES AND AQUATIC IMPACTS RC: N ERL DLTH PROJECT: 37 Leachate Toxicity Profiles for Haz. Waste Characterization PROJECT OFFICER: Gerald J. Niemi PHONE: 8-780-5511 ****************************************** PLANNED START: 01/31/88 PLANNED END: 09/30/95 ****************************************** PROJECT DESCRIPTION; GOAL: To develop leachate toxicity profiles which accurately predict the hazards to aquatic lifeforms and alteration in subsurface transport. RATIONALE: Current technology permits the measurement of potency of constituents or whole leachates to a variety of lifeforms. There is no acceptable protocol to extrapolate these data to aquatic impacts and no methods to accurately forecast the modification of whole leachate toxicity due to the chrcnatographic effects of subsurface transport. This project will provide a new profile which expands current practices to fill these voids. APPROACH: The toxicity profile will be expanded to meet the minimum needs established by the National Water Quality Data Guidelines to protect aquatic lifeforms. New methods to cost effectively assess the broad spectrum of chronic effects will be assimilated. The profile will include the distribution of toxicity in existing waste fractionation methods and will be aligned with existing subsurface transport models. PROJECT STATUS AND ACOCMPLISHMENTS TO DATE; Protocols for fractionation, identification and evaluation of toxicity in effluents will be published later this summer. These protocols have been successfully used to fractionate and identify components causing toxicity in a number of effluents. Components identified as causing toxicity in effluents include chlorine, ammonia, chelatable metals and non-polar organics. Research and development on methods for polar organics and nonchelatable metals is continuing. In addition, the possibilities of incorporating information on biomarkers in leachate toxicity profiles is continuing. An indicator of damage to mammalian DNA/RNA from toxic organic contaminants is the change in the profile of modified nucleosides released in the blood and urine relative to creatinine. A 18
------- sensitive HPLC method has been developed to characterize these nucleosides in fish. Fish from the Pox River, Wl have been collected and are being analyzed for modified nucleosides. These results will be correlated to histopathological examination of fish liver for cancerous growths. STATUS AND SCPETHTR OF nET.TVKRARTKS; 8090 DUE: 08/31/88 REVISED: COMPLETED: REPORT ON METHODS FOR LEACHATE TOXICITY IDENTIFICATION PROFILE 8092 DUE: 04/30/89 REVISED: COMPLETED: FEASIBILITY REPORT FOR THE USE OF TOXICTIY PROFILES TO PREDICT AQUATIC IMPACTS OF WHOL£ LEACHATE COMPONENTS 19
------- FEINT DATE: 08/16/88 CLIENT OFFICE OSW PMS-060 THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCKEPTIONS Office of Environmental Processes and Effects Research ERL DLTH: APRIL - JUNE 1988 ****************************************** POPE TITLE BUDGET SUB-ACnVTIY: D109 HAZARDOUS WASTE ISSUE: C WASTE CHARACTERIZATION PPA (L): 96 WASTE FUTURES AND AQUATIC IMPACTS RC: N ERL Dion PROJECT: 38 Predicting Aquatic Toxicity of HW Constituents and Exposures PROJECT OFFICER: Russell J. Erickson PHONE: 8-780-5534 ****************************************** PLANNED START: 01/31/88 PLANNED END: 09/30/95 ****************************************** PROJECT DESCRIPTION: GOAL: To develop toxic effects models, suitable for thousands of waste stream constituents, which enable aquatic impact assessment for diverse habitats and exposure conditions. RATIONALE: We have the capability to assess the toxic impact of organic chemicals and metals only under constant exposure. The uncertainties in risk assessment of this limited approach are unacceptably large. Current methods also grossly underestimate the toxicity of specific classes of waste constituents. This project provides OSW with the critical effects models to accurately assess the impact of waste stream constituents on aquatic systems. APPROACH: Aquatic impacts will be estimated based on a model which integrates fluctuating exposures with the total dose and residue in representative aquatic lifeforms. The fate of constituents within organisms will be linked to specific toxicity effects models to determine dilution factors which minimize risk. PROJECT STATUS AND ACCOMPTTSHMENTS TO DATE: A model which was developed to relate gill exchange of organic chemicals to cardiac and_,respiratory parameters, has been demonstrated to provide useful results for large rainbow trout, and is being refined. Experiments are underway to compare uptake of organic chemicals by smaller fish to relationships already established for large rainbow trout and to consumption of oxygen. Experiments are being initiated to establish rates of elimination of diverse chemicals via various routes in rainbow trout and to support development of better toxiookinetic models. The relationship of chemical accumulation to effects is being reviewed and studies on the utility of kinetic-based effects models for predicting effects of fluctuating concentrations are being initiated in cooperation with other projects. 20
------- PRINT DATE: 08/16/88 CLIENT OFFICE QPP PMS-060 THIRD QUARTER STAIUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DLTH: APRIL - JUNE 1988 ****************************************** CODE TITLE BUDGET SUB-ACTIVITY: E104 PESTICIDES ISSUE: D ECOLOGY: TRANSPORT/FATE/FIELD VALIDATION PPA (L) : 06 FIELD VALIDATION FOR HAZARD ASSESSMENT TECHNIQUES RC: N ERL DLTH PROJECT: 19 Field Validation for Hazard Assessment Techniques PROJECT OFFICER: Richard E. Siefert PHONE: -780-5552 ****************************************** PLANNED START: 10/01/83 PLANNED END: 04/30/89 ****************************************** PROJECT GOAL: Design an approach to validate current methodologies to measure pesticide impact on non-target organisms under natural pesticide use conditions. Data generated will be used to design more appropriate tests for hazard assessments. RATIONALE: Accurate hazard assessments are needed to effectively regulate pesticides. This field research will assist in validation of existing freshwater test methods as well as develop improved field protocols. APPROACH: Conduct natural pond studies using actual pesticide application procedures and determine the pesticide effects on non-target organisms. Primary and secondary (ecological) effects will be studied on microbes, algae, microinvertebrates, macroinvertebrates and fish. Environmental chemistry studies will include both water and sediment. Biota recovery studies will be conducted after pesticide applications. Results will be combined with information obtained from the literature to improve the accuracy and predictability of pesticide effects by freshwater laboratory methodology, and will provide field testing protocols. PROJECT STATUS AND AOOCMPTTSHMENTS TO DATE; A national workshop entitled "Aquatic Field Testing: Experimental Mesocosms and Field Techniques" was hosted by ERL-D on September 14-17. Attending were experts on aquatic field research from the pesticide industry, academia, private consulting firms and government agencies. Studies in 1985 and 1986 indicated that use of littoral enclosures in natural waters is feasible to improve hazard evaluation testing. Field work using multiple enclosures built in a pond and treated with three concentrations of a high use pesticide (chlorpyrifos) was accomplished. Included in this mesocosm enclosure design are controls and replications for sound statistical analyses, precise environmental chemistry (both in application of the pesticide and 22
------- analyses of the water and sediment) , and primary and secondary (ecological) effect measurements of microbes, algae, roacrophytes, microinvertebrates, macroinvertebrates, and fish. Analyses of chemical and biological samples, biota recovery studies, investigations on environmental chemistry (pesticide concentration profiles) , fish reproduction studies and other work to refine protocol were completed. A final report describing this new field testing protocol and the results of testing chlorpyrifos has been submitted to the user groups. The littoral enclosure protocol is currently being validated. The SS isomer of fenvalerate (Asana) is the pyrethroid pesticide used in this year's study. Asana is the high priority pesticide recommended to be tested by the Ecological Effects Branch of OPP. The project will result in a field testing guidance document for the Office of Pesticide Program for use in the registration of new pesticides, as well as provide ecological effects data on those pesticides tested in the littoral enclosures. STATUS AND SCff^TtTTfr QF* 7368 DUE: 09/30/87 REVISED: 03/31/88 COMPLETED; 03/31/88 FIELD VALIDATION ENCLOSURE STUDY ON JttJtotMS OF PESTICIDES IN A NATURAL FOND 7592 DUE: 09/30/88 REVISED: 03/31/89 CCMPLETED: REPORT ON FIELD VALIDATION OF ENCLOSURE PROTOCOLS FOR EVALUATING PESTICIDES ON NATURAL WATERS (N) 23
------- PRINT DATE: 08/16/88 CT.TEMT OFFICE OPP FMS-060 THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DITH: APRIL - JUNE 1988 ****************************************** BUDGET SUB-ACnvTTY: E104 PESTICIDES ISSUE: H BICTECHNOICGY/MICRDBIAL AND BIOCHEMICAL PEST CONTROL AGENTS PPA (L) : 12 DEVELOP GUIDELINE PROTOCOLS AND TEST FOR BCA EFFECTS RC: N ERL DLTH PROJECT: 06 Develop Guideline Protocols and Test for BCA Effects PROJECT OFFICER: Richard L. Anderson PHONE: -780-5565 ****************************************** PLANNED START: 10/01/83 PLANNED END: 11/30/89 ****************************************** PROJECT GOAL: Develop or improve methods that can determine the effects on freshwater ecosystems of microbial pest control agents (MPCA) . RATIONALE: Ecological risk assessment requires knowledge of an agents toxicity, concentration in the system and the populations that may be exposed after the agent enters the system. For a MPCA, knowledge of the pathology of the agent is also essential for a risk assessment. APPROACH: The pathology experiments are directed towards the organism and how it is affected by the MPCA. The toxicity program is divided in two categories. One is to develop methods that determine direct acute and chronic toxicity to non-target animals and the second is to develop methods to measure direct acute or chronic effects on populations, communities and ecosystems. The ecological studies are to develop methods to measure the relationship of the MPCA to the ecosystem and how other populations, not directly affected respond to its introduction. PROJECT STATUS AND AOOOMPTT-SHMENTS TO DATE; Our goal is_to develop acute and chronic laboratory tests for target and non-target invertebrates and fish to establish a microcosm test system that will accurately portray events in outdoor, natural systems. During the last quarter activities were divided between administration and research. Administrative activities included participation in the competitive cooperative agreement process for the Biotechnology/MPCA program and participation in the program review of biotechnology research in pesticides and toxic substances. This review was conducted on June 1 and 2 in Gulf Breeze, Florida. One result of the review was a request for additional funding and FTEs for the program. 24
------- The research activities included completion of sane studies and the beginning of new research. Completion activities included a report and paper at the 12th Symposium on Aquatic Ecotoxicology and Hazard Assessment. The paper, "Adaptation of Mixed Flask Culture Microcosms to Testing the Survival and Effects of Introduced Microorganisms" summarized the results of three nricrocosm experiments with three formulations of the MPCA Bacillus thuringiensis var israelensis (Bti). The experiments proved that the microcosm procedure is a useful method for evaluating the survival and effects of microbial spores. Based on our success with the microcosm test system, research was begun on monitoring the distribution of Bti in the water and animals in a natural pond. In this study Bti was added to the surface of the pond and samples were taken several times after the application. Animal samples were also taken and the amount of Bti in or on these animals was determined. The water and animal measurements will be compared to the data from the microcosm to aid in the calibration of the microcosm data. The pond will be sampled throughout the summer, it is now dry, to assess the toxicity of the agent to the target animal. other research activities include continuation of the uptake of Bti by fish. Acute and chronic protocols and support data for invertebrates and fish exposed to Bti are being written. These protocols will be available this fall. STATUS AND SCHFTTTTK OF DET.TWRARTES; 7254 DUE: 12/31/87 REVISED: COMPLETED: 12/31/87 REPORT ON THE MOVEMENT AND SURVIVAL OF A BIOLOGICAL CONTROL AGENT IN TWO NATURAL SYSTEMS 7674 DUE: 10/30/87 REVISED: CCMPLEIED: 10/30/87 REPORT ON FRESHWATER TESTS OF SINGLE SPECIES EXPOSED TO PESTICTDAL AND NON-PESTICIDAL MICROBES (N) 7675 DUE: 10/30/88 REVISED: COMPLETED: REPORT ON THE DEVELOPMENT AND VALIDAnCN OF SINGLE SPECIES TEST PROTOCOLS FOR PESTICIDAL AND NON-PESTICIDAL MICROBES (N) 7894 DUE: 10/31/88 REVISED: COMPLETED: REPORT ON ACUTE AND CHRONIC TEST PROTOCOLS FOR EXPOSING FRESHWATER FISH TO BCA'S 7895 DUE: 11/30/89 REVISED: COMPLETED: RPT. ON THE EFFECT OF TARGET & NON-TARGET INVERTEBRATES ON THE DISTRIBUTION, PERSISTENCE & VIRULENCE OF BCA IN FRESHWATER MICROCOSMS & NATURAL SYSTEMS 25
------- PRINT DATE: 08/16/88 CLIENT OFFICE QPP PMS-060 THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DISH: APRIL - JUNE 1988 ****************************************** CODE TITLE BUDGET SUB-ACTIVITY: E104 PESTICIDES ISSUE: I ECOLOGY: ECOTOXIdTY AND RISK ASSESSMENT PPA (L): 13 DEVELOPMENT OF INTEGRATED RISK ASSESSMENT MODELS RC: N ERL DIHH PROJECT: 32 Develop Methods of Predicting Susceptible Populations PROJECT OFFICER: Frank S. Stay PHONE: 8-780-5542 ****************************************** PLANNED START: 01/01/86 PLANNED END: 07/25/89 ****************************************** PROJECT DFy»CRIPTION; GOAL: To integrate information from toxicology, biochemistry and physiology with ecosystem models, develop a method of predicting the population most susceptible to a freshwater stressor, and develop a computerized ranking system for populations within designated communities. RATIONALE: The research in this project will compile a biological data base for a test set of 2400 aquatic species covering major taxa. The parameters will include information on anatomy, life history, ecology, physiology, and biochemistry for each organism. APPROACH: Initial efforts include extensive literature reviews of methods. Methods for comparative bioenergetics, metabolism, and detoxification are being developed to generate missing data in the second and third years. Methods of clustering organisms with respect to susceptibility parameters instead of conventional taxonomy will be evaluated. Finally a chemical-specific species ranking system will be computerized to direct subsequent risk assessments. PROJECT STATUS AND ACCOMPT-TSHMFTfTS TO DATE; A database structure was developed to include individual databases on specific areas relevant to susceptibility, related by taxonomy. This structure is being implemented under the FOCUS DBMS. Codes from the EPA TAXON system are being obtained to allow analysis of taxonomic relationships. Investigators in the research areas of toxicokinetics, toxicity mechanisms, and ecosystem recovery are identifying methods which can assist in predicting species susceptibilities and biological parameters needed to support these methods. A database on fish respiration developed earlier in this project is being incorporated into the FOCUS structure. A literature review and data coding of fish cardiac output is completed and will be entered into the database. A literature review of fish enzyme activity is in progress and will result in a 26
------- selected database at the end of FY88. Planning for a database of parameters important to species recovery is underway. A presentation, describing laboratory-to-field comparisons of the SAM and MFC microcosins, was given at the ASTM special session, Interpretation of Mirocosm Data. Microcosm studies were initiated to conpare with field studies using littoral enclosures exposed to the pesticide ASANA and to evaluate concepts on susceptibility of aquatic systems. S AND SCTTTE <">F 7470 DUE: 07/31/89 REVISED: COMPLETED: FINAL REPORT ON BIOLOGICAL DATA BASE FOR RISK ASSESSMENT 7781 DUE: 04/30/90 REVISED: COMPLETED: FINAL REPORT ON RESISTANCE AND RESILIENCE OF POND AND STREAM ECOSYSTEMS TO TOXICANT STRESS 7985 DUE: 02/28/88 REVISED: COMPLETED: 02/28/88 THE IDENTIFICATION AND EVALUATION OF AQUATIC COMUNITY ECOSYSTEM MODELS FOR USE IN ESTIMATING ECOLOGICAL RISK 27
------- PRINT DATE: 08/16/88 CLOUT OFFICE OTS FMS-060 THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DIHH: APRIL - JUNE 1988 ****************************************** CODE TITLE BUDGET SUB-ACTIVITY: L104 TOXICS ISSUE: A TEST METHOD DEVELOPMENT PPA (L) : 04 AQUATIC TOXICOLOGY RC: N ERL DLTH PROJECT: 17 Aquatic Toxicology PROJECT OFFICER: Steven J. Broderius PHONE: -780-5574 ****************************************** PLANNED START: 10/01/80 PLANNED END: 10/01/88 ****************************************** PROJECT DESCRIPTION; GOAL: To develop and validate methods for vising small aquarium fish to assess the toxicological properties of chemicals. RATIONALE: Low cost, validated assays developed on sound toxicological principles which provide data applicable for extrapolation to other species and with multiple endpoints are necessary for accurate environmental and health hazard APPROACH: Many of the in vivo assays being used to ascertain the carcinogenic potential of synthetic chemicals are very costly. Relatively less expensive assays using small fish have shown promise for establishing endpoints such as reproductive toxicity and teratogenicity. Appropriate exposure techniques and endpoint analysis designed on sound toxicokinetic and toxicodynamic principles will provide useful data on several endpoints which can be extrapolated to other species as well as provide a data base for SAR predictions. PROJECT STATUS AND AOODMPT-TSHMRNTS TO DATE; A paper entitled "Simultaneous Multiple Species Testing: Acute Toxicity of 13 Chemicals to 12 Diverse Freshwater Amphibian, Fish, and Invertebrate Families" has been published by the Archives of Environmental Contamination Toxicology, 16:697-710 (1987) . The method described was written in EPA format as an EPA Test Protocol. A draft protocol was submitted to OTS for review. The cooperative agreement deliverable #7610 on a low-cost amphibian test for specific cytotoxic tests is in its third year and testing is near completion. Due to a reprogramming of projects within the Toxics Branch the above described Aquatic Toxicology Project has been terminated and a new project dealing with validation of the medaka carcinogen assay has been established. 28
------- Deliverable 7896A has been transferred to BSAB101, Issue A, PPA 09, Project 35. STATUS AND SCBET'in'g OF DELIVERAPT3ES; 7623 DUE: 06/30/88 REVISED: COMPLETED: REPORT ON COMPARATIVE TOX. DATABASE FOR SPECIES/SPECIES EXTRAPOLATIONS AMONG AQUATIC ORG. (N) 8094 DUE: 03/31/89 REVISED: COMPLETED: REPORT ON PRELIMINARY ASSESSMENT OF USING MEDAKA FOR PREDICTING THE CARCINOGENIC POTENTIAL OF CHEMICALS 8095 DUE: 08/31/88 REVISED: COMPLETED: PROGRESS REPORT ON VALIDATION OF THE MEDAKA CARCINOGENESIS 29
------- PRINT DATE: 08/16/88 PMS-060 CLIENT OFFICE OTS THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DLTH: APRIL - JUNE 1988 BUDGET SUB-ACTIVITY: ISSUE: PPA (L) : L104 G 20 TOXICS STRUCTURE ACTIVITY RELATIONSHIPS STRUCTURE-ACTIVITY RELATIONSHIPS AND ESTIMATION TECHNIQUES RC: N ERL DLTH PROJECT: 13 Structure-Activity Relationships and Estimation Techniques PROJECT OFFICER: Gilman D. Veith PHONE: 8-780-5500 ****************************************** PLANNED START: 10/01/80 PLANNED END: 09/01/93 ****************************************** PROJECT GOAL: To develop comprehensive SAR models for the aquatic toxicology of chronic effects of industrial chemicals. The SAR models address non-specific as well as specific toxicity mechanisms for new and existing chemicals. RATIONALE: Fewer than one percent of chemicals on the TSCA inventory have been tested and many of the PMN chemicals have no test data. To screen for potential effects rapidly, structure-activity methods have been the only technically sound approach. The TSCA inventory is generically categorized and systematic test sets are generated for each important endpoint such as LC50 and NOEL. Molecular descriptors are generated for each chemical and structure-activity relationships derived. The relationships are validated by independent testing and provided to OTS with full documentation. APPROACH: A systematic reference data base for acute effects of chemicals was developed for industrial chemicals. The reference data set is used to develop mechanism-specific SAR models. Representative chemicals for each mechanism are being selected for the development of a high quality chronic effects data base which will validate SAR models for growth and reproduction effects in aquatic organisms. PROJKC1' STATUS AND ACCOMPT.TSHMRNTS TO DATE; The theoretical research on predictive toxicology has been somewhat delayed while the critical short-term objectives are being attained. The in-house research is completing phase I. QSAR methods for ecotoxicity, QSAR work on acute and chronic toxicity and specific modes of action is progressing on schedule, with extensive participation by OTS staff. 30
------- Accortplishments 1. The computerized system for QSAR evaluation of chemicals was delivered to OTS two months ahead of schedule. CLOGP 3.3 was provided for state-of-the-art Log P calculation as well as an expert system for chemical evaluation. 2. A major report on electxcfiiile-nucleophile profiles of industrial chemicals was completed. 3. A method to draw and analyze structures of reactive chemicals in three dimensions from SMILES was perfected for QSAR. 4. A file of natural substrates in living organisms has been compiled to define a natural products universe. 5. A QSAR model for polar narcotic chemicals has been defined. 6. We are continuing to define fish acute toxicity syndromes (EATS) and joint toxic action methodology that is used to help assign chemical structures to specific modes of toxic action. 7. Additional progress has been made in developing an expert system that will define metabolic pathways and rates of metabolism for industrial chemicals. 8. The first volume containing chronic data from 37 early life-^iistory-stage tests with fathead minnows was completed. 9. A preliminary procedure for developing ecotoxicity profiles for SARA Title III chemicals has been developed through an interface of the ERL-D QSAR system and the AQUIRE data base. STATUS AND SCHEDULE OF 7911 DUE: 05/31/88 REVISED: COMPLETED: 05/31/88 REPORT ON FATHEAD MINNOW CHRONIC TOXKHTY DATA BASE FOR QSAR MODELS OF ECOTOXICnY 7912 DUE: 07/31/88 REVISED: COMPLETED: PROGRESS REPORT ON SAR METHODS OF PREDICTING METABOLISM FROM CHEMICAL STRUCTURE 7913 DUE: 08/31/89 REVISED: COMPLETED: FINAL REPORT ON SAR METHODS OF PREDICTED METABOLISM FROM CHEMICAL STRUCTURE 7915 DUE: 10/31/89 REVISED: COMPLETED: REPORT ON METHODS TO COMPOTE REACTIVI'IY PARAMETERS FOR ELECTROPHILES 31
------- PRINT DATE: 08/16/83 rr.TTMT OFFICE CIS PMS-060 IHT.T' -'JJARTER STATUS REPORT CM FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DIJTH: APRIL - JUNE 1988 ****************************************** CODE TITLE BUDGET SUB-ACTIVITY: L104 TOXICS ISSUE: I ECOLOGY: ECOTOXECrrY AND RISK ASSESSMENT PPA (L): 26 DEVELOP METHODOLOGIES FOR ECOLOGICAL RISK ASSESSMENT RC: N ERL DLTH PROJECT: 31 Predicting Ecosystem Resiliency PROJECT OFFICER: Steven F. Hedtke PHONE: 8-777-2492 ****************************************** PLANNED START: 10/01/06 PLANNED END: 09/01/91 ****************************************** PROJECT DESCRIPTION; GOAL: Develop risk assessment protocols for ecosystems through the study of the dynamic response of aquatic conraunities to various contaminants and perturbations. RATIONALE: The base program addresses methods of assessing impacts of toxicants on aquatic ecosystems. This initiative will also provide predictive and field assessment methods for judging recovery period in a given aquatic ecosystem. ERL-D is currently studying aquatic ecosystem impacts in lakes, ponds, streams, mesocosms and microcosms. Although current evidence suggests that contaminant effects can be predicted from laboratory data, many factors influencing responses in the field are absent from lab tests. APPROACH: Studies in the systems mentioned above are evaluating the accuracy of predictions derived from lab tests, as well as looking for a limited number of additional indications of detrimental effects. Two complementary studies have recently been initiated as part of the Ecological Risk Assessment Initiative: 1) a literature survey and data analysis of factors controlling recovery of ecosystems from damage; and 2) a field study intended to advance our understanding of important and sensitive community and ecosystem level properties that should be protected to maintain ecosystem health. PROJECT STATUS AND ACCOMPLISHMENTS TO DATE; Of the >120 case studies on recovery that have been identified, about 75% of the systems are small (<4th order) streams with most of the remainder consisting of small lentic systems (<300 hectares). The most common impacts reviewed are acute toxic (e.g., DDT and rotenone) and chronic physical (e.g., logging and channelization) forms of stress. A variety of endpoints for measuring recovery have been reported. Most common endpoints relate to fish (n>150) and macroinvertebrates (n>400). Unfortunately, there is little consistency in the measurements of recovery. In spite of these inconsistencies, 32
------- it appears that most systems are relatively resilient because recovery times were usually less than 3 years. Exceptions include when (1) the disturbance resulted in physical alteration of the existing habitat, (2) residual pollutants remained in the system, or (3) the system was isolated so that recolonization was suppressed. A field study of related research is being conducted via an IAG with the Oak Ridge National Laboratory. STATUS AND SCPFJTT OF 7465 DUE: 08/31/88 REVISED: COMPLETED: REPORT ON METHODS TO DETEEMINE ECOSYSTEM RECOVERY PERIODS 33
------- PRINT DATE: 08/16/88 CLIENT OFFICE OAR OPD PMS-060 THIRD QUARTER STATUS REPORT ON FY'88 PROJECT DESCRIPTIONS Office of Environmental Processes and Effects Research ERL DISH: APRIL - JUNE 1988 BUDGET SUB-ACTIVrrY: N104 MULTI-MEDIA ENERGY ISSUE: E UNDERSTAND & QUANTIFY AQUATIC EFFECTS PPA (L): 05 WATERSHED MANIPULATION PROJECT RC: N ERL DLTH PROJECT: 25 Watershed Manipulation Project PROJECT OFFICER: John G. Eaton PHONE: -780-5557 ****************************************** PLANNED START: 10/01/83 PLANNED END: 09/01/92 ****************************************** PROJECT DESCRIPTION; GOAL: Determine the early indicator, as well as later, more dramatic chemical and biological responses of a warmwater bass lake to acid additions causing 0.5 pH unit reductions (from 6.0 to 4.5) every other year; evaluate the current state of the art of predicting acid effects; use results to substantiate assessments of impacts inferred from lab or survey data; obtain mechanistic data needed for acid effects modeling; evaluate the use of lab data for predicting acid effects on fish populations in the field. RATIONALE: Experimental studies on whole ecosystems are a powerful technique for determining acid effects; very few studies have been conducted; chemical and biological results will be relatable to conditions in other lakes in the U.S. and Canada; mechanistic data result in stronger models than correlative data; no lab data validation studies have been conducted for low pH. APPROACH: Acidify one-half of a clear, warmwater, low alkalinity lake in northern Wisconsin after dividing it with a removable plastic barrier; reduce the pH over 6 years after a 2-year baseline study; compare extensive preacidi- fication effect predictions with observed results; conduct lab bioassays to help elucidate organism- and population-level acid effects, and to explore organism-level sublethal response indicators. PROJECT STATUS AND ACCOMPT.T.jrS TO DATE; Ambient pH in the acidified basin of Little Rock Lake was reduced from 6.0 to 5.6 for two years, and the second of two summers of studies at 5.1 is underway. Observations from last summer's work consist largely of enhancement of effects seen at pH 5.5, e.g. extensive blooms of noxious algae, increased water clarity, shifts in lower organism populations, and direct and indirect effects on fish populations. These findings are summarized in a series of manuscripts submitted for journal publication and in fulfillment of 3/31/88 »A' milestones. The project is on schedule and has resulted in a list of over 50 non-EPA 34
------- research reports and publications. The project cooperative agreements were recently renewed for the remainder of the acidification study phase. A series of laboratory toxicity tests was completed in which the early life-stages of three Little Rock Lake fish species were exposed to low pH and Al in simulated LRL water. Additional follow-up lab and in-situ work is underway this summer. These results are expected to be extremely useful in interpreting field observations. STATUS AND SCHTTTfTTR OF 7324 DUE: 03/31/88 REVISED: OCMPLEIED: 03/31/88 JOURNAL ARTICLE: FISH POHJLATION CHANGES AND MECHANISMS ASSOCIATED WITH CHANGES IN ACIDIFIED LAKE 7325 DUE: 03/31/88 REVISED: COMPLETED: 03/31/88 JOURNAL ARTICLE ON RESPONSE OF LITTLE ROCK LAKE TO ARTIF. AdDIF. WATER CHEMISTRY, PRODUCTIVITY, NUTRIENTS, HYDROLOGY 35