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Vietnamese Crab Soup Recipes
Corn and Crab Soup Recipe
This crab soup , simply called súp cua in Vietnamese, was my attempt to finish the leftover crab from last night's dinner. We bought six crabs for the two people who actually eat seafood in our house, so there was a fair amount unfinished. I prepared cua rang me (Vietnamese tamarind crab) and that's basically all we ate with a little salad on the side. We were so full by the end of the meal that we could not finish the dish. It was our Mount Everest and we didn't conquer it!
I didn't want to waste the delicious crab, so I cracked the rest and gathered the crab meat. I had chicken stock in the freezer and I thickened it with a little corn starch. I added frozen corn and green onions to the soup and the result was just as I expected: mildly sweet, velvety and wonderfully soothing. Simply perfect!
Full Recipe...
Bun Rieu Cua Recipe (Vietnamese Crab Noodle Soup)
This soup, called bún riêu, is typical Vietnamese comfort food. It's paired with the usual Vietnamese aromatic herbs and topped with other vegetables and fried tofu for a complete meal.
Just thinking about this seafood dish makes my mouth water! Tomorrow, my cousin Tri and his wife Tran are coming over to greet us for the Lunar New Year. So I spent today making a delicious, though a tad time-consuming (but oh-so worth it) soup dish for them. Bún riêu cua is a briny crab rice noodle soup garnished with feathery-like crab cakes in tomato and fermented shrimp (called mắm tôm) broth. Once you're ready to serve, shredded lettuce and mint finish the dish for added crunch along with lime and a drizzle of nuoc mam.
If you love crab, this dish is ideal for you. If not, you could always try the veggie version (bún riêu chay) I make for the rest of my family members who are vegetarian. Either way, what a great way to start the year!
Full Recipe...
Vietnamese Asparagus Crab Soup (Sup Mang Cua Recipe)
Súp măng tây nấu cua (white asparagus crab soup) was always a festive dish when I was a child. We would have it whenever my parents had non-Vietnamese friends to our house for dinner. I would help with cracking the crab and gathering the crab meat. Corn starch, used as a thickening agent, gives the velvety, thick broth gives the dish a unique texture. While white asparagus doesn't provide a lot of color contrast to the crab, it does complement the flavor quite well.
The authentic version of this soup requires shark fin, but given how expensive it is, crab alone is used instead. The dish still has a luxurious quality, and as such, white asparagus crab soup often appears on the menu at Vietnamese weddings. Now you don't have to crash a Vietnamese wedding to try this dish; make it the next time you throw a dinner party, and I guarantee it will be the highlight of the meal.
Full Recipe...


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Player Profile
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Born 10/21/1993
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subset: commoncrawl


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I have to admit that I really wanted to get to the Keynote session on Thursday, but I was delayed by a bit of a work-related issue that had to be taken care of so it just didn't happen. But, as I have always said, everything happens for a reason. I had the opportunity to enjoy breakfast with a great group of genealogists, including Linda and Margel from the Midwest Geneabloggers group. We got into a lively conversation about how genealogical societies need to work with historical societies to leverage one another and to ensure that the level of customer service we receive as researchers is 'up-to-snuff.' There are just far too many stories of Archivist's who seemingly go out of their way to prevent researchers from accessing material they need. I don't understand this pervading and insidious way of working. We also discussed the importance of securing primary documentation as a source of genealogical material and the best methods for doing that. Is this starting to sound familiar? If not, read on…
I made it over to the Exhibit Hall as it was opening and wandered around checking out the booths. I didn't do a super thorough job, but enjoyed watching the people and talking to a few of the vendors. I got information on my Dad's (potential) SAR chapter at the SAR booth; poked around in the books available at several vendors; spoke with another Midwest Geneablogger, Kathryn Lake Hogan at the GSG booth; and had a heart-wrenching conversation with someone at the DAR booth.
I started my conversation with the DAR representative like this: Is there a National edict of what to do with the primary documentation (hand written meeting minutes, pamphlets, committee reports, etc.) held by the individual Chapters? If not, what are you doing to preserve that information from your Chapter? (Note: This particular Chapter is within the range of the Allen County Public Library) As it turns out, they decided to have their materials digitized, and they did. They kept one 'set' on several local hard drives and also have a copy on the Cloud.
Are you wondering what they did with those original books lovingly written by their mothers, grandmothers, aunts, and cousins? I was too. They shredded them. That's right, you read that right. They shredded them. Because no one wanted them or had the space to store them. Now, before you get too upset, there was some material that was given to the ACPL, but even they were reluctant to take it. I keep thinking, what is this world coming to when a LINEAGE SOCIETY destroys primary documentation? At the same time, I have a 'oh, well…' kind of mind set. I'm only one person and I there's not a lot I can do alone. But just an hour before I learned this, I was sitting at a table with a half dozen women who were ardently describing their concern about primary documentation being lost. WHAT IS THE DISCONNECT?!!
I wandered off to go to a session being presented by Kris Rzepczynski, the Senior Archivist at the State Archives of Michigan. He was the first speaker on Librarians' Day and I really liked his style, so I decided to go and hear some happy news about researching in Michigan. The room was about half way full. Kris did a great job in describing the records that are available in the State as well as a bit of the history of the migration of people into Michigan. Overall it was a terrific presentation and it genuinely made me want to get in my car and go to Lansing to see what I can find on my Powelson and Garret(t) lines.
I met up with the usual suspects and we went over to a hotel for a light lunch and some great conversation. Diana Ritchie(http://randomrelatives.blogspot.com/) shared an incredibly funny newspaper article she'd found the night before (while the rest of us were downstairs talking shop). Hopefully she'll blog about it…it's a hoot!
As the 2:00 sessions were getting ready to start, I was standing out in the hallway with quite the conundrum. Elizabeth Shown Mills was doing her "Smiths and Jones" presentation, Debbie Parker Wayne was doing a presentation titled "Going Nuclear: DNA Discoveries to Trace All Lines of Descent", and there was a guy presenting on Beginning Swiss Research. As I looked at the syllabus, I realized the only presentation that wasn't being recorded was the one on the Swiss Research so in I went. Best decision. Ever. The presentation was given by Michael Lacopo. Oh, did I mention the ladies I had breakfast with were from Granger, Indiana. Michael Lacopo? Granger, Indiana. Small world.
A couple of great take-aways with regard to the research itself, including the 1676 edict attaching the location of the person (gemeinde), at that moment, to the person and their future family permanently. How cool is that? And confusing, because if you ask someone where they're FROM they'll answer with that location, but if you ask them where they LIVED they might give the location they were actually living OR the gemeinde. Uh oh…
Michael got a rousing round of applause when he stated that everyone in the audience should hug the Archivists who work so hard to make material available to them even though they don't get paid very much to do it. OK, maybe I was the only one clapping, but still. However, I thought back to the conversation we had in the morning, and it made me wonder how many people in the audience had had a negative experience in a repository. Huh.
Lastly, as I sat marveling at the manner in which Michael Lacopo got his message across, using humor to keep the audience alert and attentive, I was struck by the thought that of any session that should have been recorded, it was his. Not enough of the lesser known speakers get acknowledged through the various Social Media channels. So, please think about that the next time you go to a Conference. Yes, it's fabulous hearing ESM, Dr. Jones and the individuals in our profession at that level, speak. But the content in the sessions given by some of the lesser known presenters is just as valuable, and in some ways more so. So, if you ever get to meet Michael Lacopo, give him a hug from me. J
The evening ended with another amazing meal at a Ft. Wayne restaurant called Gas House. They seated us in a small room at a large table and the conversation was lively, loud and full of laughter. What an inspiring and invigorating way to end the day, and I truly felt there wasn't a way that it could be any better.
As we all sauntered back to our hotels (it was a fairly long walk), we saw Amy Johnson Crow and Curt Witcher. For those who aren't aware, Curt is the Allen County Public Library Genealogy Center Manager. I struck up a conversation, telling him how impressed I was, both as a researcher and as an Archivist, with the way the Center is lead. Not managed, but lead. What ensued was a remarkable conversation about the state of Archives and Archivists, Libraries and Librarians, and education as a whole. I felt like this gentleman, who has an incredible job with immense responsibility, allowed me insight into why the ACPL is such an incredible repository. And, it gave me hope that I may still have a professional future, perhaps not one I expected, but a professional future all the same.
Speaking of futures…I'm late! LOL Off to sessions. If you have difficulties locating anyone I've discussed here, please leave me a comment and I'll get their information for you (sorry, but this blogger has GOT to go!!)


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Cell, Vol. 61, 1359-1363, June 29, 1990. Copyright ©1990 by Cell Press
Michael J. Lewis, Deborah J. Sweet, and
Hugh R. B. Pelham
MRC Laboratory of Molecular Biology
Hills Road
Cambridge CB2 2QH
Luminal ER proteins carry a signal at their C terminus that prevents their secretion; in S. cerevisiae this signal is the tetrapeptide HDEL. Indirect evidence suggests that HDEL is recognized by a receptor that retrieves ER proteins trom the secretory pathway and returns them to the ER, and a candidate for this receptor is the product of the ERD2 gene (see accompanying paper). We show here that presumptive ER proteins from the budding yeast K. lactis can terminate either with HDEL or, in the case of BiP, with DDEL. S. cerevisiae does not efficiently recognize DDEL as a retention signal, but exchange of its ERD2 gene for the corresponding gene from K. lactis allows equal recognition of DDEL and HDEL. Thus the specificity of the retention system is determined by the ERD2 gene. We conclude that ERD2 encodes the receptor that sorts luminal ER proteins.
Proteins that reside in the lumen of the ER are characterized by a C-terminal signal that prevents their passage along the secretory pathway (for review see Pelham, 1989). The optimal signal in animal cells is the tetrapeptide KDEL, whereas in Saccharomyces cerevisiae it is HDEL. Proteins bearing this retention signal are able to leave the ER by vesicular transport but are specifically retrieved from the Golgi or a pre-Goigi "salvage" compartment. In yeast, the retrieval system is readily saturable; it is presumed to involve a membrane-bound receptor that cycles between the Golgi and the ER (Dean and Pelham, 1990).
In an attempt to identify components of this sorting system, and in particular the presumed HDEL receptor, we have isolated yeast mutants that fail to retain HDEL-tagged proteins in the ER (Pelham et al., 1988; Hardwick et al., 1990). These mutants define two genes, one of which (ERD2)is a good candidate for the receptor. In support of this, we have found that the capacity of the retention system is controlled by the level of ERD2 expression: low levels of ERD2 protein result in secretion of ER proteins, while high levels render the system resistant to saturation (Semenza et al., 1990).
The definitive property of a receptor is its ligand specificity. Thus one way to test whether ERD2 encodes the receptor is to identity differences in the specificity of the retention system between species, and see whether they are determined by the ERD2 gene. We report here that one of the major ER proteins of the budding yeast Kluyveromyces lactis , namely BiP, has at its C terminus the sequence DDEL, although at least one glycoprotein in this species carries the HDEL signal. S. cerevisiae does not efficiently retain fusion proteins that terminate with DDEL, but replacement of the ERD2 gene with the equivalent one from K. lactis allows efficient retention of both DDEL and HDEL. This result argues very strongly that the ERD2 protein is indeed the receptor responsible for retrieving ER proteins from the Golgi.
K. lactis BiP Lacks HDEL
We have raised anti-peptide antisera that are specific for a C-terminal HDEL sequence. These antibodies recognize two different peptides whose only common sequence is HDEL (Hardwick et al., 1990), but will not bind to HDEL when it is present at an internal position, nor to KDEL. In a search for a suitable organism whose ER proteins have a signal different from those in S. cerevisiae, we used these antibodies to probe blots of total proteins from the yeasts Schizosaccharomyces pombe and Kluyveromyces lactis (Figure 1). K. lactis had at least one glycoprotein that was detected by the antiserum; from its mobility and abundance, it seems likely that this glycoprotein corresponds to the grp94 protein of animal cells. A smaller, unglycosylated protein was also detected (Figure 1). However, only an extremely faint signal was detected in the position expected for K. lactis BiP, implying that it carries a divergent retention signal. S. pombe contained no abundant proteins that reacted with the antiserum (Figure 1), and thus this species must also use a retention signal other than HDEL.
Figure 1. Divergence of the ER Retention Signal
in Different Yeasts
Samples of total protein from the indicated yeasts were incubated with endoglycosidase H to remove high mannose oligosaccharides. Endoglycosidase H-treated and control samples were immunoblotted with anti-HDEL antibodies. X and Y refer to the glycoproteins previously identified in S. cerevisiae; Y probably corresponds to grp94 (Hardwick et al., 1990). Staining of the blot revealed similar amounts of protein in each lane.
K. lactis and S. cerevisiae are related budding yeasts, and genes from one are usually efficiently transcribed and correctly spliced in the other (e.g., Salmeron and Johnston, 1986; Desher et al., 1989); in contrast, many S. pombe genes are nonfunctional in S. cerevisiae. K. lactis thus seemed a suitable organism with which to test the hypothesis that the ERD2 gene encodes the HDEL receptor.
Cloning the K. lactis BiP Gene
To identify the retention signal on K. lactis BiP, we cloned the corresponding gene. Low stringency hybridization with parts of the S. cerevisiae BiP gene failed to distinguish the K. lactis BiP gene from other members of the hsp70 family. K. lactis DNA was therefore digested with EcoRI and electrophoresed on an agarose gel, and DNA from regions corresponding to individual cross-hybridizing bands was eluted, cloned, and sequenced. The third fragment sequenced showed clear homology with BiP and was used to isolate a full-length clone. From this, we obtained sequence corresponding to the C terminus of the protein (Figure 2).
Figure 2. C-Terminal Sequences of K. lactis
and S. cerevisiae BiP Proteins
Asterisks indicate identical residues; two gaps have been introduced into the K. lactis sequence to maximize homology The complete sequence of the K. lactis gene will be reported elsewhere.
Strikingly, the C-terminal tetrapeptide of K. lactis BiP is DDEL. The presence of an aspartic acid in place of the histidine found in S. cerevisiae accounts for the failure of our anti-HDEL antibodies to recognize the protein.
Cloning of K. lactis ERD2
Probing Southern blots of K. lactis DNA at low stringency with the S. cerevisiae ERD2 gene revealed a single cross-hybridizing sequence, which could be localized to a 1.25 kb EcoRI-HindIII fragment. This fragment was cloned and sequenced (Figure 3A). The gene contained a single intron in the same location as the intron in the S. cerevisiae gene, and the encoded proteins showed 59% identity, with many of the changes being conservative in nature. Furthermore, expression of the K. lactis gene in S. cerevisiae was sufficient to complement the growth-arrest phenotype of an ERD2 deletion mutant. It thus seems clear that the K. lactis gene that we have isolated corresponds to the ERD2 gene of S. cerevisiae.
Figure 3. Sequence of K. lactis ERD2
(A) Nucleotide sequence of the gene. Underlining indicates important features: the initiation codon; splice donor, branchpoint, and acceptor; and termination codon. The sequence illustrated is sufficient for full ERD2 function.
(B) Alignment of the S. cerevisiae (upper) and K. lactis (lower) ERD2 protein sequences. Asterisks indicate identical residues, and charged amino acids are indicated. Two spaces have been introduced into each sequence to maximize homology. Bars above the S. cerevisiae sequence indicate a 15 amino acid duplication.
A comparison of the two sequences is shown in Figure 3B. One difference is that the 15 amino acid duplication near the N terminus of S. cerevisiae ERD2is barely recognizable as a repeated sequence in K. lactis. Moreover, the D --> N change at amino acid 51, which causes a strong erd phenotype in S. cerevisiae, is naturally present in the heterologous gene. At the C terminus, three hydrophobic stretches are very highly conserved, although the hydrophilic sequences flanking them are more divergent. These hydrophobic residues may form part of a conserved transmembrane structure.
Changing the Specificity of the Retention System
To determine the effects of ERD2 on the specificity of the retention system, we expressed fusion proteins carrying at their C termini the potential retention sequences FEHDEL (corresponding to S. cerevisiae BiP), YFDDEL (K. lactis BiP), or SEKDEL (rat BiP). Control constructs lacking these sequences terminated with SEEDLN. Initial experiments used a fusion protein containing the enzyme invertase. Secretion of this protein can easily be monitored by assaying unlysed cells for invertase activity; intracellular enzyme is inaccessible to substrate under these conditions, but can be detected after cell lysis.
Figure 4. Effect of ERD2 on the Secretion of Invertase Fusion Proteins Bearing Different C Termini
The percentage of total invertase activity that had been secreted was determined for fusion proteins terminating with SEEDLN (O), or with the same sequence followed by SEKDEL (K), FEHDEL (H), or YFDDEL (D).
(A) Wild-type S. cerevisiae strain with its normal chromosomal copy of ERD2.
(B-D) Isogenic strains that have the chromosomal ERD2 gene deleted, and carry the following plasmids: a high copy number plasmid (PER220) with the S. cerevisiae ERD2 gene (B), a low copy number plasmid (LE22) expressing K. lactis ERD2 (C), and a high copy number plasmid (LE21) with the K. lactis ERD2 gene (D).
Values (average of four or more determinations) have been normalized to the control construct (O) to aid comparison; this corrects for minor differences in overall secretion efficiency, which reflect the slightly different growth rates of the strains. About 30% of the invertase activity was secreted from the control strains.
As shown in Figure 4A, the HDEL- tagged fusion protein was secreted less efficiently from a wild-type strain than were the KDEL or DDEL versions, neither of which showed significant retention relative to the control. Thus, neither KDEL nor DDEL is efficiently recognized by the S. cerevisiae receptor under these conditions.
A slightly different result was obtained with a strain that lacked the chromosomal copy of ERD2 but instead carried the gene on a multicopy vector. Retention of the HDEL construct was improved, but in addition partial retention of both DDEL and KDEL was observed (Figure 4B). Thus when the capacity of the system is increased by overexpression of ERD2, its apparent specificity is broadened. Presumably, under normal conditions the receptor is saturated with endogenous HDEL-containing proteins, which effectively compete with the DDEL and KDEL sequences, but when receptor is present in excess, a weak affinity for the latter sequences is revealed. Nevertheless, with both high and low levels of ERD2 expression, HDEL was the preferred substrate.
A different result was obtained when the plasmid-borne ERD2 gene was replaced with K. lactis ERD2. Two different plasmids expressing the K. lactis gene were used; one was a low copy number vector with the ERD2 coding sequences fused to the TPI promoter, while the other was a high copy number plasmid carrying the gene with its own promoter. Strains containing these plasmids showed different efficiencies of retention, presumably because they expressed ERD2 at different levels, but in each case the specificity was the same: invertase fusion proteins bearing HDEL and DDEL were retained equally well, while the KDEL construct was secreted as efficiently as the control (Figures 4C and 4D).
As a further test, we examined the secretion of prepro-a factor fusion proteins in strains lacking
the chromosomal ERD2 gene and carrying either S. cerevisiae
or K. lactis ERD2 on a multicopy plasmid. Pro-a factor is secreted rapidly, and the fusion
proteins can most easily be assayed by pulse-labeling and immunoprecipitation.
Molecules that pass through the Golgi are proteolytically processed
and thus escape detection, so the amount of labeled protein remaining
after a short chase gives a direct indication of the efficiency
of retention (Dean and Pelham, 1990; Semenza et al., 1990).
Figure 5. Effect of ERD2 on Secretion
of Pro-a
factor Fusion Proteins
Fusion proteins bearing the same termini as those in Figure 4 were assayed by pulse-labeling and immunoprecipitation.
(A) Strains carrying the chromosomal ERD2 deletion and high copy number plasmids expressing either S. cerevisiae or K. lactis ERD2 were labeled for 10 min and chased for 15 min.
(B) Deletion strains carrying the low copy number plasmid expressing the K. lactis ERD2 gene were labeled for 10 min and chased for 5 min.
Arrows indicate pro-a factor The lower band is the ER form, and the upper band corresponds to protein that has cycled through an early Golgi compartment.
We conclude from these experiments that the ERD2 gene controls the specificity of the ER retention system. S. cerevisiae ERD2 allows strong recognition of HDEL and weak recognition of DDEL and KDEL; K. lactis ERD2 allows recognition of HDEL and DDEL, but not KDEL. The dual specificity of K. lactis ERD2is consistent with the presence of both DDEL and HDEL on ER proteins of this organism.
Our aim in these experiments was to test the hypothesis that the ERD2 gene encodes the receptor that sorts luminal ER proteins. We first looked for a species of yeast with a receptor specificity different from that in S. cerevisiae, then isolated its ERD2 gene, transferred it to S. cerevisiae, and tested for a change in the specificity of the retention system. The results indicate that the pattern of retention is indeed determined by the ERD2 gene.
Divergence of the Retention Signal for Luminal ER Proteins
We were surprised to find that the BiP protein of K. lactis had the C-terminal sequence DDEL, because it implies considerable divergence of the retention signal between two related budding yeasts. All known examples of luminal ER proteins in S. cerevisiae have HDEL at their C terminus; these include BiP (Rose et al., 1989; Normington et al., 1989; Nicholson et al., 1990), protein disulfide isomerase (M. F. Tuite and R. Freedman, personal communication), the KRE5 gene product (Meaden et al., 1990), and two other proteins identified only by their reactivity with anti-HDEL antibodies. Proteins from other species, including plants, nematodes, insects, and vertebrates, almost invariably have a positively charged amino acid 4 residues from the terminus (reviewed by Pelham, 1989), although Plasmodium falciparum BiP has serine (Kumar et al., 1988). The occurrence of an acidic residue at this position in K. lactis BiP is thus unprecedented.
Despite this striking difference, at least one glycoprotein in K. lactis has an HDEL sequence, as judged from its ability to bind anti-HDEL antibodies. The presence of two rather different sequences raises the question of how recognition of both of them is achieved.
ERD2 Encodes the Sorting Receptor
Previous experiments had identified the ERD2 protein as a good candidate for the HDEL receptor, because its abundance controls the capacity of the retention system (Semenza et al., 1990). We have shown here that the signal specificity of the system is also determined by ERD2. With S. cerevisiae ERD2, HDEL is the preferred signal, whereas when the gene is replaced by the K. lactis homolog, HDEL and DDEL are retained with equal efficiency. This difference is independent of the level of expression of the ERD2 genes and thus cannot be explained by a mere quantitative difference in the activity of the two ERD2 proteins. Furthermore, the observed preferences are entirely consistent with the known sequences of ER proteins in the two species. We therefore conclude that ERD2 indeed encodes the receptor.
In the case of K. lactis, this single receptor must be capable of binding both FEHDEL and YFDDEL, which suggests a surprising degree of flexibility in its interactions. The specificity of the S. cerevisiae receptor is also not absolute, because when it is expressed at high levels there is some recognition of both DDEL and KDEL sequences, neither of which has been found in this species. Presumably, this reflects weak binding of the receptor to DEL. The dual specificity of the K. lactis receptor cannot, however, be explained simply by binding to DEL, because SEKDEL is not recognized.
One possibility that we cannot rule out is that binding of YFDDEL to the K. lactis receptor is influenced by the two aromatic residues upstream of the terminal tetrapeptide, but there is no evidence that more than 4 amino acids are recognized in other species. We therefore prefer a model in which HDEL and DDEL each interact with the receptor in a highly specific manner.
The ERD2 protein not only functions as a receptor, but is also required for normal vesicular traffic through the Golgi (Semenza et al., 1990). If ligand binding involves parts of the protein that are also required for viability, it will be difficult to identify the binding site by mutation. The results in this paper suggest that it may be possible to identify the sequences responsible for ligand specificity by creating chimeric ERD2 genes with sequences from S. cerevisiae and K. lactis. It should also be possible to identify conserved functional motifs by isolating the receptor gene from more distantly related species. Very recently, an anti-idiotypic antibody approach has identified a 72 kd glycoprotein that is good candidate for the KDEL receptor in animal cells (Vaux et al., 1990). It will be interesting to see whether part of this protein shows similarity to the 26 kd ERD2 product.
Experimental Procedures
Derivatives of S. cerevisiae strain SEY2102 (MATa suc2-d9 ura3-52 /eu2-3,-112 his4-519) were used for Figure 4A. Other results were obtained with derivatives of the ERD2 deletion strain D209 (Semenza et al., 1990). K. lactis was kindly provided by Stephen Johnston (Duke University), and S. pombe by Andrew Newman.
The K. lactis BiP and ERD2 genes were detected by hybridization with probes containing the corresponding S. cerevisiae coding sequences, which we had isolated previously (Hardwick et al., 1990; Semenza et al., 1990). Hybridization was in 4x SSC, 10% formamide at 42°C, and filters were washed with 2x SSC at 50°C. Fragments migrating with the hybridizing bands were excised from an agarose gel and cloned into M13 vectors. The relevant clones were identified in these minilibraries by plaque hybridization.
For high level expression, the EcoRI-HindIII fragment containing the K. lactis ERD2 gene was inserted into the LEU2-containing 2µm vector ZUC13 to form plasmid LE21. Expression at a lower level was obtained by fusing the gene at the Scal site (position 280) to the TPI promoter and inserting it into a vector containing TRP1, ARS1, and CEN3 to form plasmid LE22.
Isogenic strains carrying a deletion of the chromosomal ERD2 gene and the K. lactis ERD2 plasmids LE21 or LE22, or the S. cerevisiae ERD2 plasmid PER220, were generated as described by Semenza et al. (1990).
Invertase and pro-a factor fusion constructs were identical to those used previously (Pelham et al., 1988; Dean and Pelham, 1990) except that additional constructs encoding proteins terminating with YFDDEL were prepared using synthetic oligonucleotides. All were inserted at the URA3 locus. For the experiments shown in Figure 5, only strains containing a single inserted gene (identified by Southern blot analysis) were used. Invertase assays, pulse labeling, and immunoprecipitation are described elsewhere (Pelham et al., 1988; Dean and Pelham, 1990). Note that the 2 factor constructs contain the epitope recognized by the monoclonal antibody 9E10, allowing their specific immunoprecipitation without interference from endogenous 2 factor
We thank Kevin Hardwick for performing the experiment shown in Figure 1, Jan Semenza, Stephen Johnston, and Andrew Newman for yeast strains, Neta Dean for the pro-a factor plasmids, Mark Bretscher, Barbara Pearse, and Sean Munro for helpful comments on the manuscript, and Steve Fuller, David Vaux, and John Tooze for communication of their results prior to publication.
The costs of publication of this article were
defrayed in part by the payment of page charges. This article
must therefore be hereby marked "advertisement"
in accordance with 18 U.S.C. Section 1734 solely to indicate this
Received May 10, 1990.
Dean, N., and Pelham, H. R. B. (1990). Recycling of proteins from the Golgi compartment to the ER in yeast. J. Cell Biol., in press.
Desher, J. O., Larson, G. P, and Rossi, J. J. (1989). Kluyveromyces lactis maintains Saccharomyces cerevisiae intron-encoded splicing signals. Mol. Cell. Biol. 9, 2208-2213.
Hardwick, K. G., Lewis, M. J., Semenza, J., Dean, N., and Pelham, H. R. B. (1990). ERD1, a yeast gene required for the retention of luminal endoplasmic reticulum proteins, affects glycoprotein processing in the Golgi apparatus. EMBO J. 9, 623 630.
Kumar, N., Syin, C., Carter, R., Quakyi, I., and Miller, L. H. (1988). Plasmodium falciparum gene encoding a protein similar to the 78-kDa rat glucose-regulated stress protein. Proc. Natl. Acad. Sci. USA 85, 6277-6281.
Meaden, R, Hill, K., Wagner, J., Slipitez, D., Sommer, S. S., and Bussey, H. (1990). The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (1-6)-ß-D-glucan synthesis and normal cell growth. Mol. Cell. Biol. 10, 3013 3019.
Nicholson, R. C., Williams, D. B., and Moran, L. A. (1990). An essential member of the hsp70 gene family of Saccharomyces cerevisiae is homologous to immunoglobulin heavy chain binding protein. Proc. Natl. Acad. Sci. USA 86, 1159 1163.
Normington, K., Kohno, K., Kozutsumi, Y, Gething, M.-J., and Sambrook, J. (1989). S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiR Cell 57, 12231236.
Pelham, H. R. B. (1989). Control of protein exit from the endoplasmic reticulum. Annu. Rev. Cell Biol. 5, 1-23.
Pelham, H. R. B., Hardwick, K. G., and Lewis, M. J. (1988). Sorting of soluble ER proteins in yeast. EMBO J. 7; 1757-1762.
Rose, M. D., Misra, L. M., and Vogel, J. R (1989). KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene. Cell 57, 1211-1221.
Salmeron, J. M., and Johnston, S. A. (1986). Analysis of the Kluyveromyces lactis positive regulatory gene LAC9 reveals functional homology to, but sequence divergence from, the Saccharomyces cerevisiae GAL4 gene. Nucl. Acids Res. 14, 7767-7781.
Semenza, J. C., Hardwick, K. G., Dean, N., and Pelham, H. R. B. (1990). ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway. Cell 61, this issue.
Vaux, D., Tooze, J., and Fuller, S. (1990). Network antibodies identify an intracellular membrane protein that recognises the mammalian ER retention signal. Nature 345, 495-502.
GenBank Accession Number
The accession number for the sequence reported in this paper is M34844.
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Abstract: Monumental Tombs near Troy: Recent Discoveries
This lecture focuses on a series of monumental tombs near Troy discovered during the course of the last fifteen years. There are five tombs in all, dating from the late Archaic to the Early Hellenistic period, and most of them were set up by wealthy Anatolians who were associated with the Persian governor (or satrap) at the regional capital of Daskyleion. The tombs include a marble sarcophagus (ca. 500-490 B.C.) featuring the murder of Polyxena, daughter of Priam. This is the earliest stone sarcophagus with figural scenes ever to have been found in the eastern Mediterranean. Another sarcophagus within the same tomb contained the body of a child surrounded by gold jewellery and silver symposium implements. A new sarcophagus from the modern city of Çan (ca. 400-375 B.C.) contains a biographical narrative of the deceased, showing him victorious in a boar hunt and then spearing a fallen Greek foe in the eye. Nearly all of the original paint still survives intact. All of these are Graeco-Persian products, produced primarily by Greek artists employing Persian iconography.
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Ah. Violet and white are NYU's colors, which explains half of everybody's outfits last week, and also one more dimension of how sweet Chuck is being. Neat. And it seems like he only fondles his necktie when he says the words "business" or "meeting," so there you go.
In the second home run in a row, Blair and Chuck spend the episode fighting over a photo up for bid at Sotheby's. The idea itself is silly, but the setup is valid: he needs it to impress a club owner so he can make Victrola 2.0, while B needs it for admission to La Table Élitaire, a secret salon that will prove her intellectual worth.
B, who dated Carter when she was in free-fall last year, explains to Serena that she is dating Carter because she's in free-fall this year. S doesn't believe it, even though he's acting like Chuck plus Aaron Rose. Then S, who was the subject of Georgina's obsession in the first season, tells Dan that he is going to be the subject of G's obsession this year. Lonelyboy doesn't believe it, even though he has been present for every messed-up thing G has ever done.
Scott's been spending enough time at Lily's stalking Rufus that it's making Jenny and Eric uncomfortable. With her unerring nose for other people's private stuff, Vanessa instantly gets Scott to admit he's not an NYU student, but Rufus and Lily's stalkery son. Dan and G investigate, but only figure out that he's Dan's literary stalker from last year.
Nate and Bree Buckley make faces and sounds at each other. Oh, and I'm guessing Carter had something to do with her sister-cousin's ruined wedding that she whined about that time on that farm.
V drags Scott to Sotheby's so he can out himself to Rufus, but even after his mom gives her blessing in front of a photo of Little Edie, he lies and says he's the other brother, and that he stalked Dan and dated V and took guitar lessons from Rufus because he... Was just obsessed with his dead adopted brother's biological parents for some reason? Scott and V hug and cry so long their mascara runs, and G figures out that he's Pilot Inspektor.
S realizes Chuck and B have been setting Carter up to look like a heel in order to protect her, so she buys the photograph out from under them both and tosses her hair around a lot. Eventually she gives the photo to B, who gives it to Chuck, who rediscovers his inner fire. S realizes the whole Table Élitaire deal -- and thus the whole C/B war -- was a Georgina scheme, so Dan dumps her.
To the summery tones of Miike Snow's "Animal," then, Chuck decides to cash out of Bass Industries altogether and buy a hotel on his own, which sounds scary; Bree threatens to use Nate/Serena to hunt Carter down, maybe literally, in order to get back in her family's favor; S brings Carter to a van der Humphrey breakfast; G heads to Boston to destroy/extort/enlist Pilot Inspektor; and there's still no Lily, Jenny or Eric happening. On the other hand, Tyra Banks and Montana McGuire next week.
For more from Television Without Pity:


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WATCH: Plaintiffs in landmark Utah marriage case get engaged
February 18, 2014
This weekend, on Valentine's Day, Derek Kitchen gave his partner Moudi Sbeity a romantic night to remember in their home of Salt Lake City, Utah when he got down on one knee and asked for his hand in marriage.
Of course, Derek and Moudi have been talking about marriage for months now - in fact, they are one of the couples who brought a lawsuit against the state of Utah seeking the freedom to marry for same-sex couples in the Beehive State. In December, U.S. District Judge Shelby ruled in their case, Kitchen v. Herbert, and found that denying same-sex couples the freedom to marry is unconstitutional. For nearly three weeks - until the U.S. Supreme Court granted the opposition's request for a stay in the ruling - same-sex couples across Utah had the freedom to marry, and in that time, more than 1,300 couples received marriage licenses.
Derek and Moudi weren't one of those couples - they are waiting until their marriage rights are 100% secure, once and for all, but as they continue working on their case - which faces a hearing by the 10th Circuit Court of Appeals on April 10, 2014 - they couldn't help but join in on the romance and get officially engaged.
Derek proposed at the Equality Ball, which benefitted Marriage Equality USA and Restore Our Humanity, the legal group who brought the historic case to court. Since the December 20 ruling, the National Center for Lesbian Rights has taken on the case as co-counsel. In his proposal, Derek explained:
Although we've been plaintiffs in this case, we haven't been able to do the traditional thing people do with marriage - a proposal. When we decided that we were going to sue the state, we knew that it was the right thing to do - and that's how Moudi and I make decisions. We lie in bed, and we talk about it, and we express our concerns and excitements and we figure it out together. That's how decisions are made in our house. So we never went through with the formal process.
And so, because I have a platform tonight, and because I'm feeling a little bit excited about where we're headed, into the 10th Circuit this April, I wanted to offer you a ring - and I want to ask you if you will make me the happiest man ever.
Watch the proposal below:


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In a move that could provide a boost to the small but growing digital comic book business, DC Comics has signed deals to sell comics through online stores owned by Apple, Amazon and Barnes & Noble.
Previously, comic book readers could only buy digital copies of issues to read on tablets or other digital devices through DC's own app, or an application controlled by the company ComiXology.
While graphic novels have previously been available to buy for digital devices through a variety of stores, DC is the first company to offer single comic book issues through Apple's iBookstore, Amazon's Kindle Store and Barnes & Noble's Nook Book Store.
The agreements mean readers can, starting on Wednesday, get copies of comic books such as Superman, Batman, and the Justice League from the digital stores that come built-in to the the iPad, Kindle and Nook.
Growing sales of digital comic books is critical for Warner Bros.-owned DC, its major competitor Marvel (owned by Walt Disney Co.), and smaller publishers as sales of print comic books are in the midst of a long-term decline.
-- Ben Fritz, Los Angeles Times


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Paul Wolfowitz
Paul Dundes Wolfowitz (born December 22, 1943) is an American academic, neoconservative scholar and political figure; In the early 1970s, Paul Wolfowitz served as an aide to Democratic Party Senator Henry M. "Scoop" Jackson; from 1986 to 1989, he served as ambassador to the Republic of Indonesia; then as undersecretary of Defense under Ronald Reagan; from 2001 to 2005, he served as the Deputy Secretary of Defense under George W. Bush where he was one of the main architects of the foreign policy of the George W. Bush administration and of the Iraq War; from 2005 to June 2007, he served as the President of the World Bank. He is currently a visiting scholar at the American Enterprise Institute.[1] Wolfowitz described himself as a "Scoop Jackson Republican".
Paul Wolfowitz's father Jacob Wolfowitz, was a Professor of Mathematics at Cornell University. Paul Wolfowitz has a bachelor's degree in mathematics from Cornell University; a master's degree and Ph.D. in political science from the University of Chicago.[2] From 1994-2001, Paul Wolfowitz served as Dean and Professor of International Relations at the Paul H. Nitze School of Advanced International Studies of The Johns Hopkins University.[3] Lewis "Scooter" Libby was one of his students when Wolfowitz served as a professor of Political Science at Yale University.[4]
Eric Schmitt described Paul Wolfowitz as a "soft-spoken former aspiring-mathematician-turned-policymaker … [whose] world views … were forged by family history and in the halls of academia rather than in the jungles of Vietnam or the corridors of Congress … [His father] … escaped Poland after World War I. The rest of his father's family perished in the Holocaust." David Dudley described Wolfowitz as "liv[ing] in a world haunted by atrocities" and deeply affecting his son's personal and intellectual development. Speaking directly of the influence of the Holocaust on his own world-views, Wolfowitz said: "That sense of what happened in Europe in World War II has shaped a lot of my views … It's a very bad thing when people exterminate other people, and people persecute minorities. It doesn't mean you can prevent every such incident in the world, but it's also a mistake to dismiss that sort of concern as merely humanitarian and not related to real interest."
In August 1963, Paul Wolfowitz joined the March on Washington for Jobs and Freedom led by Martin Luther King, Jr.
Tenure As President Of The World Bank
Other nations have been critical of Wolfowitz, and the criticisms intensified to calls for his ouster upon disclosure that in April 2007 he allegedly obtained a large pay raise and promotion for his girlfriend, Shaha Ali Riza, a former bank official.[5] He resigned on May 17, 2007 (effective June 30, 2007), as a result of an investigation by the World Bank Group's board of executive directors, which accepted his resignation, "ending a protracted and tumultuous battle over his stewardship, sparked by a promotion he arranged for his companion."[6][7]
Lewis D. Solomon, Van Vleck Professor of Law at George Washington University, has recently published a biography of Paul Wolfowitz titled Paul D. Wolfowitz: Visionary Intellectual, Policymaker, and Strategist.[8]
- Bush's Brain Trust by Sam Tanenhaus, Vanity Fair, July 2003.
- 'I'm Not a Unilateralist': Paul Wolfowitz Wants the World to Understand Him by Lally Weymouth, Newsweek, April 4, 2005.
See also
- That Bleeding Heart Wolfowitz by Christopher Hitchens, Slate, March 22, 2005.
- Paul Wolfowitz's political donations by Newsmeat.
- Communication from the Executive Directors of the World Bank Group, World Bank Group, May 1, 2007.
- With the Best of Intentions by Michael Hirsh, Newsweek, May 21, 2007.
- ↑ http://www.aei.org/scholars/scholarID.126,filter.all/scholar.asp
- ↑ http://www.whitehouse.gov/results/leadership/bio_466.html
- ↑ Paul Wolfowitz's biography at World Banks site
- ↑ http://www.guardian.co.uk/international/story/0,3604,1449631,00.html
- ↑ http://www.sfgate.com/cgi-bin/article.cgi?f=/c/a/2007/04/14/MNGHNP8M941.DTL
- ↑ "Statements of Executive Directors and President Wolfowitz", World Bank Group, May 17, 2007, accessed May 17, 2007.
- ↑ Matthew Jones, "Wolfowitz Exit Seen Clearing Way for Progress", Reuters (UK), May 18, 2007, accessed May 18, 2007.
- ↑ http://www.amazon.com/Paul-Wolfowitz-Intellectual-Policymaker-Strategist/dp/0275995879


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Wild Cub's Keegan DeWitt Talks Debut Album, Defends Dance Music + Big-Ups Blur and the Boss
Keegan DeWitt came to New York City last month with every intention of giving the city his all. The Wild Cub frontman and mastermind has a background in film score, but he's as comfortable onstage as he is in the studio, and that's fortunate, if not essential, given the nature of his latest project. Suave and poppy like Tears for Fears, slick and dancey like Prince, derivative of neither, Wild Cub kick irresistible neo-New Wave jams that have made the Nashville quintet one of this year's most buzzed-about bands.
Indeed, DeWitt left part of himself onstage in NYC -- Wild Cub's sold-out gig at Mercury Lounge had Diffuser.fm busting some regrettable dance moves -- but that's not all he left behind. Days later, he revealed via Twitter that the group had been robbed of more than half of its gear. Fortunately, DeWitt took it in stride -- perhaps because he has more important things on his mind. He and his wife are expecting their first child, due later this month, and Wild Cub have been taking meetings with record labels, planning the next phase of their career.
In an interview with Diffuser.fm, DeWitt discussed impending fatherhood, his love of dance music and his earnest performance style. He also explained his goals in writing 'Youth,' Wild Cub's recently released full-length debut.
What happened with your gear?
We added a show at the last minute, that Atlas Genius show at Webster Hall. Tuesday morning, I walked out to the van, and there was a note on the van that said, "Your van was broken into. All of your equipment was scattered all over the street. We put it in the basement of our business." [The thieves] had gone trough everything and decided what they wanted, and left the drums for some reason and a couple amps. But they'd stolen the two really expensive keyboards and all the guitars and a bunch of other stuff.
We've actually had to do this once before in New York. Our release show in August, we had our van towed with all the equipment in it. And we found out as we as we were going to drive to the show. That was a lot more harry, because it was like, "Let's find all of our equipment in the next 45 minutes." … We were joking around [this time] it was a lot easier to pack the van going home.
You've got to look on the bright side. On a related note, you're about to become a father. How might that affect Wild Cub? Will parenthood make you focus more on your film work?
It's gonna be an interesting year, because we're on the road a ton this summer. And with my score stuff, we have a film that went to Sundance that is now going to Munich and the Prague Film Festival. When the baby is about a month old, we're gonna throw her in a baby bjorn and take her to Munich and Prague, and that will be our indoctrination to figure it out.
You come to Wild Cub having done acting acting and film scores, and you've also recorded some folky singer-songwriter solo albums. This is a much more pop- and dance-oriented project -- were those influences always there, or have you recently gotten into these things?
All along, while I was doing the folky stuff, I'd always desired to be in a band in the most basic way. I really desired not only the ability to command a room -- just basic noise level in general, where you can demand attention -- but even when I was releasing the more singer-songwriter stuff, I was primarily interested in rhythm. I was really excited about dance music.
My gateway into it was that growing up, I really admired Damon Albarn from Blur. He made that Mali record [2002's 'Mali Music] and other more rhythmic Gorillaz records -- especially the later Gorillaz records get really interesting -- and that started to spark my interest. And then I would follow down all of those different pathways he would hint at, about these different African musicians or rappers. That started when I was 16, and I got to a point where it was like, "I want to make something where rhythm is the primary thing."
My primary goals were: How can I make a record where my face isn't on it, and if possible, my name isn't on it, and it allows people to just totally enjoy the cinematic aspect of it? We're creating moments. We're trying to hint at moments inside people's identity. We do that with both the image -- the record doesn't have any of our names in it, and it's just black and white with these little snapshots -- or we do it with rhythm … A lot of [the songs], I would write drums first. I was trying to totally invert how I would write as a pop musician. Rather than starting with an acoustic guitar and writing a melodic line and going, "That's really cool" and building from there," I'd start with a drum beat or a bassline and try to avoid guitars until the very end of the whole process.
In recent years, indie rock musicians have become a lot more comfortable talking about dance, pop and R&B influences, whereas once, that wasn't really the case. Do you notice a shift?
Being the person who's doing it, you constantly battle people hating dance music and thinking it's stupid. Well, I disagree, and there's a huge, long lineage of people who really disagree with that -- people as classy as [Italian producer Giorgio] Moroder who have really proven there's substance to dance music, and there's really cool stuff there and so many different routes to get there ... [On the other hand], people are like, "Indie music is really boring. You're using bar chords on a guitar. That's really boring. Why can't you be like Grizzly Bear?" It's so funny, now that I'm a 30-year-old man, to be able to look back and see how critical indie music is of itself. That's the great gift of being a little bit older, saying, "Well, guess what? F--- you guys. I dont' care. I'm making music to enjoy for myself that mirrors these emotional themes I think are really interesting, and I'm gonna explore whatever route I want to do that." And I trust people connect on the other end of that. And I find people do, which is really cool.
We were in a record label two days ago, and they were like really hip, really indie rock. I still have moments where I'm like, "I don't even know if these people like dance music." ... If it's uncool, that's fine. As long as I'm earnest about it, and I put hard work into creating it, I can make peace with it.
In terms of pulling old R&B influences, for sure, I feel that way. I don't want to say any names specifically of stuff that fuel ['Youth'], because you can pull them out yourself, but I think there's a value, too, in going, "I really like [a specific band]." For example, [the 'Youth' song] 'Wild Light': People will come up after every single show and say, "I hate to ask this, but do you like the Talking Heads?" And I'm OK with that, because that song, for me, is like my exploration of my appreciation of them. There's nothing tongue-in-cheek about it. I really enjoy it, and the lyrics have resonance for me. They're about something that's really interesting and that I feel like I've taken pride in. It's all valuable, and it's all a valuable exercise to go through and be able to retread on that stuff.
Your music is similar to that of someone like Twin Shadow, but whereas he has sort of a theatrical look and mystique about him, you're very approachable. Onstage at Mercury Lounge, you were constantly thanking the audience, and it came across as very genuine. Is that a conscious thing?
I'm unashamedly a huge fan of Bruce Springsteen, more so in terms of his work ethic: "I'm present here for you, and my purpose is to be here for you and facilitate your emotional connection to the music." It'd be different if we had a million-dollar budget, and we could do some amazing light show. Then I'd talk less and let people experience it.
You've mentioned meetings with record labels. Is signing with a major still something groups think about? Are you heading in that direction?
We've met amazing people. Everywhere we go, the people are awesome. But the overwhelming response from everyone in the music industry -- and this isn't just to us as a band -- is everybody wants you to prove you can do it 110 percent by yourself already, and then they want to work with you. And then they want to take 50 percent of your profits … If we can continue to release records 100 percent ourselves and be totally in control of all the videos that come out and all the press that comes out and the special edition vinyl -- and be able to maintain 100 percent ownership of that -- we'd like to continue to do that.
But that would change if a label -- and this remains to be seen -- steps forward before the next record and says, "We've got all these different intangible things." There are a couple labels we've met with where just having [their] name attached would go a long way to shepherding it to a whole crowd of people.
Watch Wild Cub's Video for 'Thunder Clatter'


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Author Notes: I love fruit soups, with strawberry soup and plum soup at the top of my list. I wanted to create something with mangos that wouldn't be a dessert (because we've been eating so many cookies, crisps, and cakes lately), so soup seemed like a natural choice for me. —TheWimpyVegetarian
Food52 Review: WHO: ChezSuzanne is a lifelong food lover who teaches cooking classes in San Francisco.
WHAT: A refreshing cold soup that balances fruity and spicy flavors -- perfect for summer.
HOW: If you can push the button on a blender, you can make this soup! The ingredients whizz together in no time.
WHY WE LOVE IT: Sweet, tangy, and creamy, this make-ahead soup is perfect for summer entertaining and lazy, hot afternoons alike. —The Editors
Serves: 4
3 ripe mangos
1 seven-ounce container of plain, low-fat Greek yogurt (I used Fage)
¼ cup coconut milk
¼ cup low-fat milk
1 tablespoon + 2 teaspoons lime juice
½ teaspoon salt
2 teaspoons honey (I used Buckwheat honey)
1 teaspoons mint leaves
1/2 Serrano pepper, minced
- Slice the mangos in half around the large seed in the middle. Scoop out the flesh with a spoon. Put the flesh in the bowl of a food processor fitted with a blade.
- Add all the rest of the ingredients to the bowl, and whirl the processor like crazy. If you don't have a food processor, I'm sure a blend would work fine too.
- Chill for at least an hour before serving. I found the flavors to be best when chilled overnight.
- Your Best Mangoes Contest Finalist!
- This recipe is a Community Pick!
More Great Recipes:
Soup|Fruit|Mango|Make Ahead|Spring|Summer|Vegetarian|Side


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(Posted Wed. Dec 28th, 2011)
Dec. 28: Increased export demand for U.S. pork is raising pork profitability and leading to an overall rise in the national hog inventory according to a December reports released by the U.S. Department of Agriculture's National Agricultural Statistics Service and Economic Research Service. The National Corn Growers Association welcomes this growth, which translates into stronger demand for corn and distillers dried grains.
"Corn farmers value our relationship with the hog industry, which consistently provides an important market for our product," said NCGA President Garry Niemeyer. "In 2011-12 marketing year, it is estimated that hogs will consume 960 million bushels of corn. As demand grows for pork both internationally and domestically, corn farmers benefit also thus reinforcing the importance of cooperation among all sectors in the agricultural community."
USDA Economic Research Service projects a significant increase in pork exports during the fourth quarter of 2011, with 1.4 billion pounds of pork exported during this period. This 22 percent increase over fourth quarter exports in 2010, if realized, would set total 2011 U.S. pork exports at more than 5.1 billion pounds. Further agency estimates indicate these levels will hold in 2012.
In addition to strong demand for U.S. pork abroad, domestic demand could also see a slight increase in 2012. Service estimates foresee this demand increase as the market balances against projected reductions in beef and poultry production.
The rise in demand has already increased production, which should be sustained in the coming year. NASS reports indicated that, as of December 1, the total U.S. hog inventory stood at 65.9 million head, two percent higher than at that time last year with the breeding herd expanding at the same rate during that period. While the rate of sows farrowing fell by one percent from September 1, an increase in the number of viable pigs produced per litter hit a record high of 10.02 offsetting the drop in sows.
The overall increases in pork production, export and in domestic demand have not only resulted in a rise in demand for corn, but also in demand for distillers dried grains, an ethanol co-product. Hog sector interest in and demand for DDGs increased steadily over the past few years to take advantage of the quality protein option that it offers at a low cost.
For the full report, please click here.


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Sweden's Edler suspended for remainder of World Championship
Stockholm, Sweden – Swedish defenseman Alexander Edler has been suspended for the remainder of the 2013 World Hockey Championship.
The International Ice Hockey Federation independent disciplinary panel made the announcement on Friday, one day after Edler delivered a knee-on-knee hit to Canada captain Eric Staal.
Edler collided with Staal late in the first period of Sweden's 3-2 shootout win in the tournament's quarterfinal round. Staal was in obvious discomfort, holding his right knee.
The hit earned Edler a five-minute major penalty and a game misconduct.
The disciplinary panel viewed the incident as "reckless, dangerous and that it was in disregard to the vulnerability of his opponent and for that he must be held accountable."
Sweden is set to face Finland in the semifinals on Saturday. A victory sends the Swedes to Sunday's gold-medal game. The other semifinal pits Switzerland against the United States.


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Diddy To Get Involved In Howard University Student Protest!
7 news reports "Hundreds of Howard University students gathered outside the school's administration building Friday morning to protest what organizers called the administration's failure to address student concerns."
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The last update was pretty hefty, so from now on I'll try to update with one part of the build at a time. This next part of the exterior changes includes upgrading the tail lights and adding some tail light euro guards. I've swapped out the stock tail lights with some Bermuda Black LED lights from IPCW. LED's are great because they are much brighter and last longer than normal lights, and these also look awesome with the black finish!
Now on to the Rugged Ridge Tail Light Euro Guards. These are great accessories for tail light protection and they look really good too. They fit on stock lights and same size aftermarket lights, like the IPCW LED's I've installed. They're also offered in a stainless/chrome finish.
Everything was very easy to install, more or less just a few screws and I was done!


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Lattice multiplication
Lattice multiplication is a method of multiplying large numbers using a grid. This method breaks the multiplication process into smaller steps, which some students find easier. Digits to be carried are written within the grid, making them harder to miss.
Lattice multiplication is a method of multiplying large numbers using a grid. It is algorithmically equivalent to regular long multiplication, but the lattice method breaks the multiplication process into smaller steps, which some students find easier. Digits to be carried are written within the grid, making them harder to miss.
Multiplying on a lattice
After the lattice is constructed, a round of multiplication is followed by a round of addition, much as in traditional multiplication of large numbers.
Setting up the lattice
First, draw a grid that has as many rows and columns as the multiplicand and the multiplier. The grid shown here is for multiplying a 4-digit number by a 3-digit number.
Next, draw a diagonal through each box from upper right corner to lower left corner. Continue the line a short way past the grid. (Ms. Thomas describes the process to her students as "corner to corner and out the door.")
Write one factor across the top and the other down the right side, lining up the digits with the boxes.
The multiplication is performed by multiplying the digits at the head of each row and column. Fill in each square of the grid with the product of the digits above and to its right, recording the products so that the tens are in the upper (diagonal) half of the square and the ones are in the lower half. If the product does not have a tens digit, record a zero in that triangle.
In the example shown here, the highlighted row and column give us 1×5=5, so we write 0 in the upper half of the square and 5 in the lower half.
Now add the numbers in the grid along the diagonals, starting from the lower right corner. (Ms. Thomas describes this step to her students as "ride the slide.") Carry any tens into the top of the next diagonal.
In this example, the highlighted diagonal gives us 4+2+5+1=12, so we write 2 at the bottom of the diagonal and carry the 1 to the top of the next diagonal to the left.
To find the answer, read the digits starting down the left of the grid and continuing across the bottom. Here, the answer to 2314×157 is 363,298.
Lattice multiplication of decimals
Lattice multiplication can easily be extended to multiply decimal fractions. Suppose, instead of the above problem, we wanted to multiply 2.314 by 1.57. We would proceed as before, but draw lines from the decimal points down and to the left until they meet, then follow the diagonal to the left or bottom of the grid. The point where this diagonal emerges from the grid is the position of the decimal point in the answer.
Here, the result of the multiplication is 2.314 × 1.57 = 3.63298.
Creating a lattice in Microsoft Word
Although most students can draw lattices accurately by hand, some may have difficulty. Printed lattices may also be helpful when students are first learning the process.
Drawing the lattice
- Use a large font, such as 24-point.
- On the top toolbar, click on Table and then select Draw Table. This will open the Tables and Borders toolbar.
- Click on Insert Table. Select the desired number of columns and rows (one more each way than the number of digits you plan to multiply). Set table height to 0.5". Select Finish.
- Click on Draw Table on the Tables and Borders toolbar. Use the pencil cursor and left mouse button to draw diagonal lines.
- Highlight the outside left row. Use the Border button to remove the outside border and horizontal lines. Repeat with the bottom row to remove the outside border and vertical lines.
Manipulating the lattice
Copying. To copy a table, select the entire table, right click, and select Copy. Move the cursor to another part of the page, right click, and select Paste.
Moving. To place tables side by side, rest the cursor on the upper-left corner of the table until the table move handle appears. Right click and drag the table using this handle.
Enlarging. Rows and columns can be inserted to increase lattice size. Click on Table, then Insert, and choose either rows or columns.


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Oxwich Bay Hotel Wedding Photographer
Amy & Scott | 7/06/14
Oxwich Bay Hotel Wedding Photographer
Amy & Scott | 7/06/14
Follow Christopher Barroccu Wedding Photographer on
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© Christopher Barroccu Photography Limited.
Cardiff Wedding Photographer and Swansea Wedding Photographer, Christopher Barroccu offers professional, creative and contemporary Wedding photography.
Christopher Barroccu covers South Wales, the UK, and the rest of the World through his Destination Wedding Photography service.
Christopher is a professional, international wedding photographer who is passionate about the art of wedding photography.
Over the last few years Christopher has developed a photographic style which specializes in unique, distinctive and exceptional wedding photography that is timeless, romantic and above all captures your personality.


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Commercial Property Maintenance Roseville
Let's start with something that is a little off topic. Say, you have lots of money and you decide to purchase a very expensive car. But what about after making the purchase? You might have heard old people saying there is nothing special in buying an expensive car, the real challenge is of maintaining. The same rule applies everywhere. You can build a huge complex by spending a good amount of money but your job will not be done by just doing that. A plant only becomes tree when it gets proper maintenance. You cannot expect a commercial property to make money for you without needing maintenance.
Do not take this thing lightly. Maintaining a commercial place is not as simple as it looks. There will be occasional damages in the need of urgent repair. On special days, you will also feel the need of renovating the interior and exterior of your commercial building. Managing everything on your own will certainly put a lot of pressure on your brain. But wait! You can avoid the headache be simply outsourcing everything. Monster contractor is here to help you with your commercial property maintenance Roseville CA.
Our property maintenance staff in Roseville knows what it takes to provide your property the maintenance it needs. Our Commercial Property Maintenance Roseville is ready to help. Call Now (916) 550-4381 We have team of skilled and experience workers who are constantly guided by experts at every point. Our area of expertise not only includes maintenance services but we also deal with construction work like renovation the exteriors and interiors. When your property needs a professional maintenance, Monster Property Maintenance is your contractor. You can always count on us for efficient progress and high quality workmanship. Commercial property maintenance service in Roseville, Monster provides exterior and interior general construction, repair and maintenance services.
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ECQA has signed an Agreement with the Japanese SPI Consortium
ECQA and JASPIC (Japan SPI Consortium) have signed agreements to deliver this qualification to Japan. A first training of trainers took place in Japan.
To start the collaboration a training of trainers took place in Tokio, Japan, 27.-29.7.2015. JASPIC members (experts from NEC, NTT Data, Hitachi, Fujitsu, Sony, Canon, Toshiba, SRA, etc.) actively participated.
* * * * *
ECQA has become a Certifier for INTACS
ECQA is since November 2014 certifier for iNTACS (International Assessor Certification Schema, www.intacs.info) for ISO 15504 assessors in the non-Automotive areas. The news from 6.2.2015 show a picture with a team of of T-Systems in Germany who attended an ECQA exam for ISO 15504 competent assessor training.ECQA also does exams for ISO 15504 IT service assessors at Volkswagen from 2015 onwards. INTACS has currently 1200 assessors worldwide and with leading electronic and Automotive industry in the board.
* * * * *
ECQA has been nominated a Best Practice in KA4 by the EU
ECQA has been nominated as a best practice of the LLP program, and was published in the brochure Good practice KA4.
Social Partnership Strategy, download from here...
A first paper addressing the demographic problem and offering a
solution by the ECQA approach was published by the IEEE (largest publisher
of the world, conference in Spain hosted by United nations university) in
July 2012. Download from here...
A further paper was published as a joined paper of the EuroSPI
community (www.eurospi.net, http://2014.eurospi.net) in the technical
proceedings, June 2014. Download from here...
* * * * *
Upcoming event & CfP
ECQA Days 2015
The ECQA Days 2015 will take place in Ankara, this time, they are organised as an integrated associated event of EuroAsiaSPI 2015, from 30. September - 2. October 2015.
Specific sessions of EuroSPI 2015 will be marked as ECQA sessions. Contributions from ECQA will focus on key European qualifications needed to empower an innovative working environment and also new skills currently needed in industry for innovation and improvement. More...
CALL for PAPERS
Papers shall
- address case studies where specific skills have been applied for improving and innovating companies.
- describe core competencies needed in industry to maintain leadership in innovation and engineering.
|First Paper Submission
|Review by Committee and Notification of Acceptance
|Final version of paper
|Early registration Deadline
|Upload of Conference Presentation
Innovation and Communication
Social Skills
Learning Strategies
Engineering Aspects
Functional safety
Integrated Design
Quality Design
Management aspects
Project management
Quality Alliances and Networks
Submission procedure
Papers are submitted as a EuroSPI industry track paper. The same submission dates apply. First Paper Submission until 25.05.2015.
Papers submitted shall carry a title preceded with ECQA (e.g. ECQA Functional Safety Design Skills in Automotive).
Review Committee
Chair: Michael Reiner, Univ. of Applied Sciences Krems, Austria
Contributions will be reviewed by an international group of experts:
Andreas Riel, Grenoble INP, France
Jørn Johansen, DELTA, Denmark
Tomas Schweigert, SQS, Germany
Klaudia Dussa Zieger, Methodpark, Germany
Risto Nevalainen, SPINET, Finland
Tomi Rozman, BICERO, Slovenia
Janos Ivanyos, Trusted Business Partners, Hungary
Damjan Ekert, ISCN, Austria
Blanca Nájera, Termnet, Austria
Andrea Fenz, Skills International, Austria
Miklos Biro, SCCH, Austria
Ricardo Colomo Palacios, Carlos III University Madrid, Spain
Elli Georgiadou, Middlesex University, UK
Richard Messnarz, ISCN, Ireland
Gabriele Sauberer, Termnet, Austria
ECQA Newsletter No. 3/2013
HEI-UP team wins "Special Award" - The best innovative project (BPM Summit EE 2013)
Expert in project management, business process management, information technologies and entrepreneur
ECQA is happy to announce, that the ECQA international project team has won the "Special Award - The best innovative project (BPM Summit EE 2013)" for our HEI-UP project (Improvement of BPM in Higher Education Institutions).
The award means our 2-year work is recognized by experts in this field. This recognition will surely give our project another boost - in the terms of the dissemination and attracting future attendees to the course we've developed.
Read more... and see in LinkedIn...
ECQA Newsletter No. 2/2013
Newsflash, ECQA News, ECQA Events, Training Schedulemore...
How to Cooperate with ECQA?
You are interested in attending one of our courses? Browse through the Skill Cards here.
You are interested in becoming an ECQA trainer? More information you can find here.
Your organisation is interested in becoming an ECQA training provider? More information you can find here.
- You are interested in becomin an ECQA member or ECQA JRC member? More information you can find here.
Find details about related conferences and events in Europe here.
Open your mind and become a member of an Europe-wide collaboration and strategy.
ECQA Days 2013 ǀ Sept. 26 - 27 in Cannes, France
ECQA Days 2013 - Let's join our strengths together
The ECQA Days took place from Sept. 26th - 27th in Cannes, France. Participants from several different European countries joined us to spending two great conference days with us. The program was a great success and offered valuable insights into current projects in various fields.
The conference day started off with a keynote from Prof. Alain Bernard,PhD, followed by presentations of Dr. Richard Messnarz, Dr. Dick Theisens, Dr. Andreas Riel, Darragh Coakley, Dr. Kerstin Siakas, Errikos Siakas, Dr. Nathalie Gormezano, Dr. Tomislav Rozman, Prof. Frieda Steurs and further keynotes of Dr. Gabriele Sauberer and Dr. Christian Kreiner.
The workshop day continued with another very interesting keynote from Dr. Dick
Theisens and continued with interactive workshops including impulse notes from Dr. Christian Kreiner, Risto Nevalainen, Dr. Dániel Pálosi, Ricardo Colomo-Palacios,
PhD, Prof. (FH) Michael Reiner, Jørn Johansen and Dr. Gabriele Sauberer.
We greatly appreciate every participant's effort to travel to Cannes and to spend
two great days of exchanging ideas and networking with us.
For more details and some fotos ...
Eden Hôtel Cannes
133, rue d'Antibes
06400 CANNES, France
Program details
ECQA Conference Day 1 - International Speakers and Key Notes
ECQA Conference Day 2 - Morning and Afternoon Workshops
ECQA Days Final PDF Program for Download


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Cabo San Lucas, Los Cabos Guia de Viagem
Cabo San Lucas Resumo
- Beautiful beaches -- one of the things Cabo San Lucas is known for (only second to, of course, partying!)
- Stunning historic sites, like the stone Iglesia de San Lucas, established in 1730
- Beautiful and famous rock formation at Land's End
- Whale migration from January to March
- Party central: tequila; conga lines; dancing; swim-up bars; the whole sha-bam!
- Known to be Mexico's elite resort town; abundance of luxury hotels within the area
- Plethora of adventure activities like snorkeling, diving, and surfing
- Popular with tourists, but still a compact and walkable town
- Lots of traditional Mexican restaurants, especially on the Marina
- Cabo resort towns sometimes lumped together as all being extremely similar and non-authentic
- Very "Americanized"
- Expensive resorts
What It's Like
At the southern tip of Mexico's Baja Peninsula, Cabo San Lucas -- a beach town on the stretch of coastline collectively known as Los Cabos (The Capes) -- has long been a popular vacation spot for sun-worshipping party seekers. (Hence, the nickname Cabo San Loco.)
One reason why Los Cabos remains a staple in the beach resort world is its California-esque vibe. It almost feels like an extension of Southern California, with similar deluxe accommodations, striking golf courses, and high-end shops.
All posh-ness aside, Cabo San Lucas still presents visitors with stunning scenery and a distinct Mexican charm, especially around the coastline. The large cluster of rock formations on Land's End is famously beautiful, and there's great whale watching January through March (particularly in March). Old churches, charming restaurants, and handicraft vendors prove that this town is more than just a wild spring-break getaway.
Where to Stay
Visitors looking to dive head first into all the action should aim for the Downtown area, Cabo San Lucas' unrivaled tourist center. Those seeking a more authentic (or as authentic as you can get in Cabo!) experience may want to check out hotels in the Marina. This fishing area offers more interaction with locals. Water sports enthusiasts should look to stay around Medano Beach, which offers plenty of water sport activities and equipment rentals. Meanwhile, Pedregal Hills is a safe and quiet residential area (home to many U.S. citizens), which might be better for families.


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Welcome to Alejazu Google Map
Alejazu Google Map is for informational use only, no representation is made or warranty given to its content. User assumes all risk of using free Alejazu Google Map on Satellite City Maps.com. In case you have any suggestion about Alejazu map, we are glad to hear about it!
Uploaded pictures of Alejazu
Link to Alejazu Google Map page:
There is a very easy way how to link to our Alejazu map - including free Google map search, country, city maps and facts, Google route planner -, ready to use on your website. Please, simply copy & paste the provided HTML code into the source code of your page. Thank you for all links to Satellite City Maps.com!
|Text link to: Alejazu google map


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Results 1 to 10 of 14
#1 Kerry: We're Talking About An "Unbelievably Small" Effort In Syria09-09-2013, 09:10 PM
SECRETARY OF STATE JOHN KERRY: Now, I believe that the aftermath of the Iraq experience and Afghanistan leave a lot of people saying, "We dont want to see our young people coming back in a body bag," and so forth. But thats not what were talking about. And what we have to do is make clear to people that this is were not talking about war. Were not going to war. We will not have people at risk in that way. We will be able to hold Bashar Assad accountable without engaging in troops on the ground or any other prolonged kind of effort in a very limited, very targeted, very short-term effort that degrades his capacity to deliver chemical weapons without assuming responsibility for Syrias civil war. That is exactly what were talking about doing unbelievably small, limited kind of effort.
Video of ketchup queen at link>http://www.realclearpolitics.com/vid..._in_syria.html
We're talking small, miniscule, Blud Lite so to speak. The same great killin' but a lot less fillin"
This message brought to you by Blud Lite a subsidiary of Red line in the Sand Breweries."No, I'm not getting rid of ICE like how would we keep drinks cold"
Alexandria Ocasio Cortez
It's easy to say "What could go wrong?" before you roll the snowball off the mountain, blow up that old case of dynamite that's probably no good anyhow, or drill a hole in that 3' diameter pipe in your backyard to see what's inside.
But wait!!!
Could Fearless Leader and John F'n Kerry suddenly pull Victory out of defeat???
Will the Ebil Commie Putin put the squeeze on Assad???
Will Assad give his ChemTrail Toys to the (Shudder) U.N.???
Will Duck Dynasty ever be watched by Linda#'s????
Tune in tomorrow, same Bat Time, same Bat Channel!!!
* This update brought to you by Dow Chemicals. Life is all chemicals...
May the FORCE be with you!
Here Are the Top 14 (Mostly Brutal) Reactions to Kerrys Unbelievably Small Remarks on Syria
Sep. 9, 2013 3:12pm Becket Adams
Secretary of State John Kerry on Monday said a U.S. military strike on Syria would be limited and unbelievably small, words that must have struck fear in the heart of Syrian President Bashar Assad.
We will be able to hold Bashar Assad accountable without engaging in troops on the ground, or any other prolonged kind of effort, in a very limited, very targeted, very short-term effort, Kerry said at a joint press conference with British Foreign Secretary William Hague in London.
Kerry added that it would be an unbelievably small, limited kind of effort.
Considering Kerry was speaking in favor of U.S. involvement in Syria, some were left scratching their heads over his word choices.
Would an unbelievably small military strike actually accomplish something in the war-torn country? Would Assad even take that threat seriously?
Needless to say, the reaction from observers was not favorable.
Here are the top 14 reactions (in no particular order):
14. ABC News Jonathan Karl wrote: Heres an unusual way to instill fear into the heart of your enemy.
13. Rep. Mike Rogers (R-Mich.):
I dont understand what he means by that, Rep. Rogers, whos in favor of a military strike on Syria, said Monday on MSNBC.
I dont think describing the size or effort of what our target sets are or what ship fires what missile is in our national security interest, candidly. And again, this is part of the problem.
Thats a very confusing message certainly a confusing message to me that he would offer that as somebody who believes this is in our national security interest, he added.
12. Conservative commentator Bill Kristol:
I am worried, though, the administration has done such a bad job of making its case, the Weekly Standard editor said Monday. John Kerry in London to reassure the British and Europeans said were not talking about war. It would be very targeted, very short-termed. Then he said it would be an unbelievably small, limited kind of effort.
Kristol, who favors intervening in Syria, said Kerrys remarks have shaken in his support.
Now we have the secretary of state saying, Well, we went to Congress, it was so important to go to Congress, for an unbelievably small limited strike, he said.
Even I can see why reasonable people on the Hill, and I think its unfair to characterize them all as isolationists or irresponsible, reasonable people on the Hill can say is that really better than nothing?
11. Human Events John Hayward called it a sound bite that will linger in history.
10. Slates Joshua Keating wrote [emphasis added]:
I may not have much experience with brinksmanship, but it seems to me that threatening to hit someone becomes a lot less effective when at the same time youre telling your friends, Dont worry, Im not going to hit him that hard. And convincing the public that this situation is analogous to the buildup to the largest war in human history is difficult when youre also saying that an unbelievably small effort will be sufficient to deal with it. Given the blows the Assad regime has already absorbed over the last two years, its hard to imagine statements like these changing his thinking.9. But not everyone was so hard on Kerry. The Atlantics James Fallows offered the following defense:
I realize the administration is speaking to multiple audiencestrying to play bad cop with Assad at the same time its trying to cajole a skeptical American public. But when the same official is playing both roles, it becomes a little hard to keep the narrative straight.
You know what he meant. He was advancing the argument for a contained, surgical, pinpoint, etc., effort that would be big enough to let Assad and future dictators know the cost of using chemical weapons, yet not so broad as to entrap the United States in the ongoing (horrific) civil war. If the two words that had come to his mind in real time had been unbelievably precise rather than unbelievably small, no one would have blinked.And then there was the reaction on Twitter. As per the norm, it was pretty brutal:
8. Sen. John McCain (R-Ariz.):
Kerry says #Syria strike would be "unbelievably small" - that is unbelievably unhelpfulabout 12 hours ago via webReplyRetweetFavorite
@SenJohnMcCainJohn McCain
7. National Reviews Charles C. W. Cooke:
This remark is very Bidenistic."No, I'm not getting rid of ICE like how would we keep drinks cold"
Alexandria Ocasio Cortez
I have to wonder if John Kerry didn't first hear this phrase uttered by his wife, perhaps on several occasions and it some how slipped out during his speech."No, I'm not getting rid of ICE like how would we keep drinks cold"
Alexandria Ocasio Cortez
So, we've gone from "Shock and Awe" to "Aw shucks?"
Meanwhile, the administration is in the process of training mosquitoes (non-malarial, of course) to bite Bashir Assad on the back of his knees, which will itch terribly. That'll show him.--Odysseus
Sic Hacer Pace, Para Bellum.
Before you can do things for people, you must be the kind of man who can get things done. But to get things done, you must love the doing, not the people!
And we can trust the U.N. and Putin. Right???May the FORCE be with you!
09-10-2013, 11:57 AM--Odysseus
Sic Hacer Pace, Para Bellum.
Before you can do things for people, you must be the kind of man who can get things done. But to get things done, you must love the doing, not the people!
09-10-2013, 01:24 PMSolve a man's problem with violence and help him for a day. Teach a man how to solve his problems with violence, help him for a lifetime - Belkar Bitterleaf
Liberalism is what the stupid think is smart.
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Event box
Lapsit Music and Movement at NE!
Have fun moving with stories and music! Learn basic musical concepts and enjoy exciting and active stories at the Northeast Regional Library every Wednesday. We'll incorporate several movement disciplines including yoga. No pre-registration or equipment needed. Please wear clothing that is comfortable for active play or dance. Appropriate for ages 18 months and younger.
NE Youth Services Department
- Date:
- Wednesday, Dec 20th 2017
- Time:
- 9:30am - 9:50am
- Location:
- Oak Room
- Campus:
- Northeast Library
- Categories:
- Children's Programs Early Literacy Programs


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Movie 'Argo' Gets Iran Riled Up to Produce Own Movie–YIKES!
You had to know that sooner or later some official/s in the country of Iran was going to respond to the movie 'Argo,' which won for best picture at the Oscars last night.
Apparently, Iran is going to produce its own movie, and as we all know, they are the movie capital in the world. What can we expect? We'll have to wait and see.


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Test your English
This page is only available in English.
How to use this test:
Add your contact details below. Then select the word or phrase which you think is correct from the drop-down menus. There are forty [40] questions, and depending on your proficiency in English, this test will take between 5 and 30 minutes. When you have finished, click 'Submit'. You will then receive your score.


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Product Overview
The Kaspa from Weaver features the rugged durability you expect from a Weaver scope with an affordable price tag. The one piece 1" tube is 100% waterproof and allows ample mounting space. The 44mm objective lens provides a crisp clean sight picture and is fully multi-coated for extra light gathering. The Kaspa features a fast focus eye piece, side focus finger adjustable turrets, includes protective lens covers and is covered by Weaver's limited lifetime warranty. Weaver has designed an outstanding scope for the serious hunter or shooter that is looking for quality at a price that won't break the bank.