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ZAATARI, Jordan — At Jordan's Zaatari refugee camp near the border with Syria, the horror of the neighboring country's civil war can be seen in the faces of its youngest refugees.
More than 50,000 refugees under the age of 18 call the wind-swept, massive desert camp home. All have stories about the war, like 11-year-old Amal Qalloosh, who fled her home near the city of Daraa with her family after a government bombing.
"On the way here, there was a lot of shelling," Qalloosh said. "It was terrifying but we made it all safe to the camp."
Some children work in Zaatari, while the lucky attend school at the camp. As Muslims celebrated Eid al-Fitr this week, marking the end of the holy month of Ramadan, some children received new clothes from aid agencies and played, though life in the camp can be hard.
"I have no more control of my children," said Amani Sbaihi, a mother of seven whose two brothers fought with rebel forces and were killed by Syrian government troops. "They are getting old so young. It's so difficult to have authority over them in such an environment"
More than 2.8 million Syrian children inside and outside the country — nearly half the school-aged population — cannot get an education because of the devastation from the civil war, according to the U.N. children's agency, UNICEF. That number is likely higher, as UNICEF can't count the children whose parents didn't register with the United Nations refugee agency.
UNICEF estimates more than 10,000 children have died in the violence. Others suffer from emotional problems after experiencing the war.
Associated Press photographer Muhammed Muheisen captured these photographs of young Syrian refugees inside Jordan's Zataari refugee camp.


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Asked by Dan, South Carolina
During a recent family trip to the mountains, our kids, who were sleeping in the attic, were awakened by a bat flying around the room. We called our doctor, who told us to go to the emergency room, where the kids got the rabies vaccine and immune globulin. Was that really necessary?
Living Well Expert
Dr. Jennifer Shu
Children's Medical Group
Thanks for your question. In hindsight (such as following a negative rabies test on the animal in question) it often does seem that rabies prevention shots are unnecessary.
However, doing the injections as a precaution is a very reasonable recommendation. That's because rabies infection is almost always fatal without the treatment.
Fortunately, most animals are not infected with rabies, so transmission to humans is rare, affecting just a handful of people every year. Bats are one of the most common animals that transmit rabies in the United States, along with raccoons, skunks, foxes and coyotes.
The rabies virus can be found in all parts of the country except for Hawaii.
If there is concern about an exposure to rabies, prophylaxis consisting of rabies immune globulin (an injection that contains human antibodies against the rabies virus) and rabies vaccine (four injections over the course of two weeks) is recommended.
This treatment should be started as soon as possible after the exposure, ideally within 24 hours.
Bat bites reportedly feel like sharp jabs and may leave tiny marks that look like scratches. Most adults who were awake during the time of a bat bite will know they have been bitten.
However, if a person is uncertain whether a bite or scratch from a bat occurred (such as if a bat was found in the room of a sleeping person, a child, a person with mental disabilities or someone who is impaired by the influence of alcohol or drugs -- basically anyone who is unable to give an accurate report about a bite), the rabies prophylaxis regimen is recommended.
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subset: commoncrawl


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Patagonia Men's Torrentshell Jacket
Regular price $129.00
Aside from stunning landscapes, Southern Patagonia is known for one thing: wet weather. For trekking and hiking in drenching conditions, our Torrentshell Jacket provides H2No Performance Standard protection that's durable, lightweight and versatile. The 2.5-layer nylon jacket has a waterproof/breathable barrier, a DWR (durable water repellent) finish and stuffs into one of two zippered handwarmer pockets (with a carabiner clip-in loop). The hood rolls down, stows away and has a laminated visor, the center zipper has minimal welt exterior and interior storm flaps and the ventilating pit zips have storm flaps and DWR-treated zippers. With a microfleece-lined neck, hook-and-loop cuff closures and a drawcord hem.
- H2No Performance Standard shell with waterproof/breathable 2.5-layer nylon ripstop
- 2-way-adjustable hood with a laminated visor rolls down and stows
- Microfleece-lined neck provides comfort and protects waterproof/breathable barrier
- Center-front zipper features minimal welt exterior and interior storm flaps that create a zipper-garage chin guard
- Two zippered handwarmer pockets and venting pit zips; all with welted exterior storm flaps and DWR (durable water repellent)-treated zippers
- Self-fabric hook-and-loop cuff closures and adjustable drawcord hem seal out weather
- Self-stuff handwarmer pocket with carabiner clip-in loop
- H2No Performance Standard shell: 2.5-layer, 2.6-oz 50-denier 100% nylon ripstop, with a waterproof/breathable barrier and a DWR (durable water repellent) finish
- 345 g (12.2 oz)
- Made in Vietnam.
2.5-layer H2No Performance Standard shell fabrics are waterproof, windproof, breathable and highly packable. They combine a water-repellent shell fabric with a waterproof barrier and a raised pattern on the inside, instead of the interior scrim used in 3-layer fabrics. The raised pattern makes 2.5-layer fabric light and compressible, while still protecting the waterproof barrier.


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I have been thinking about retirement and what will I do then. Thought of becoming a professional genealogist occurred. But, then I had an eureka moment, how can I find others ancestors when I can not find my own.
I must admit that there is the thrill of discovery when I find a new tidbit. So, now I am thinking, I had better put that idea on a backburner and concentrate on finding my own ancestors.
I think what hampers me the most is the fact that my ancestors did not come over on the Mayflower. The first to emigrate came to the USA in 1874, so there is no Civil War records for me to search, or don't even toy with the idea of researching for the DAR, or First Families of Pennsylvania.
I have found the emigration, ship arrivals and census records and there they are all neat and tidy. Actually, I think I am descended from boring people who stayed put in one place. Sort of like me, actually.
I have found Irish records back to my great grandfathers (Thomas Dowd) birth in 1860, and the record of his parents (Thomas Dowd and Cate Sweeney) My other Irish set (O'Rourke) seem elusive. I have an obit that says they are in County Limerick, but Denis is next to impossible to find, not to mention is wife Mary ?????
The Germans are another enigma. I can not find the city that my great grandfather Heinrich Theodor Frederich Schridde, was born. I suppose the lack of progress has me down but I must keep on plodding.


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Just minutes after the doors opened to the 2013 Local Food and Wine Challenge, getting from one table to the next was an effort riddled with, "excuse me, pardon me." A woman next to me in line said, "No matter where you go. you're bumping into people but, hey, the more people drink, the less they'll care!"
The second annual event thrown by the Ventura County Wine Trail crowded the ballroom at the Mandalay Beach Hotel and Resort in Oxnard with hundreds of people, all gathered to enjoy, learn from and judge 12 food and wine pairings crafted by teams of local winemakers and restaurants. Coveted decanters etched with "The People's Choice" and "The Judge's Choice" awaited the two teams whose pairings were most appreciated. And any twinge of guilt that might arise from two and a half hours of ponderous face-stuffing was countered by the proceeds of the event going to FOOD Share of Ventura County.
True to both the evening's wintry weather and the ranching heritage that sculpts so much of Ventura County's favored flavors, many of the 12 pairings were small offerings of huge, gamey tastes and local vegetables matched with bold red wines. Veal, for instance, was used in two restaurants' dishes — Tierra Sur's green peppercorn veal sausage (paired with a dry Herzog Meritage) and Café Zack's osso bucco (paired with a robust Lodi Barbera from Sunland Vintage Cellars).
Austen's at the Pierpont Inn also played in this field with slow-roasted pork tenderloin and fruit compote, bringing subtle barbecue rub flavors that were brightened with a tangy dijonnaise sauce and Gone West Cellar's cola-y Paso Zinfandel. So did Osteria Monte Grappa, with a squab risotto with nettles and mushrooms that shone with an unfussy yumminess alongside Ojai Vineyard's Syrah.
But the crowning glory of the gamey flavors faction was the rustic wild boar stew served by Bella Victorian Vineyard Tasting Room and Bistro, which included pancetta, root vegetables and winter mushrooms. With deep, nuanced flavors akin to beef bourguignon that its Camarillo Estate Syrah complemented perfectly, this pairing was a favorite that took home the Judge's Choice Award.
The second annual Local Food and Wine Challenge brought in
hundreds of attendees, with the proceeds benefiting FOOD Share,
Ventura County's food bank.
Ventura's notoriety as a seafood region was well-represented by two very different diver scallop preparations. One from Aloha Steakhouse was simple and fresh-tasting, featuring English cucumber topped with pickled beets and a slice of seared scallop, while another from neighboring C-Street Restaurant took on notes of tropical decadence with its whole scallop topped with mango-grapefruit relish and drizzled with chive oil.
Jolly Oyster, of course, also culled from the sea, serving two very interesting oyster hors d'oeuvres that veered wildly from on-the-half-shell approach. An olive-oyster tapenade had a remarkably structured flavor progression, ending with a mild black pepper heat that was well-tempered by Malibu Family Wine's crisp Saddlerock sparkling brut. The other appetizer also carried striking flavors; oysters were brined in brandy, soy and garlic, smoked, then served on a leaf of endive with tzatziki sauce.
Other restaurants did a great job finding inspiration from beyond our borders. Sugar Beets Restaurant & Bar in Oxnard cobbled together Asian and European influences with its marinated duck breast served on a chili wonton with cherry compote, roasted hazelnuts and chocolate-grenache drizzle. With this sweet-savory dish, Plan B Cellars' grenache was transformed from round, mouth-flooding juiciness to racy, dark plum skin acidity.
Twenty88's short ribs had an Asian flair as well — crisp at the edges but moist from a spicy cherry-ginger glaze — and the drying start on Cantara Cellars' Intrepid blend cut through that boldness and then made a whole bite segue into satisfying red-fruit smoothness, earning this team the People's Choice Award.
Obviously, this event brings a ton of indulgence value, but peppered between many thoughts along the line of "Oh my God, yum" were many surprising, enlightening experiences. As the night wore on, the event took on an educational quality as I was continually humbled by the realization that there's so much more going on in the local food and wine scene than I ever knew of.
For instance, I figured local icons like La Dolce Vita and Ojai Vineyard would be there, presenting their popular creations. But I knew nothing about other restaurants, like Twenty88 in Camarillo and Sugar Beets in Oxnard. Same with many wineries, including the new Plan B Cellars in Ventura and Cantara Cellars in Camarillo. Yet all of their offerings were impressive, leading me to realize that going to Santa Barbara on the weekends is foolish when there's so much worth discovering that is so much closer.
Also, every team brought knowledge and insight with clever pairings. Typically, I'm prejudiced against the sweetness of gewürztraminer but when Magnavino Cellars' was paired against the mango-grapefruit relish on C-Street Restaurant's diver scallops, the fruit negated the wine's sweetness and brought forward a crisp, refreshing element. And Mourvèdre is always a mystery for me, as it's more often used as a blending grape, but with Rancho Ventavo's I learned that this varietal's brooding flavors can pop with a silky fruitiness when served with a rich spiced meat like the cumin pulled pork served by Hollywood Beach Wine Company.
The best analogy for the meaning of a good pairing that I've heard came from talking with Raeann Koerner, professor of health and kinesiology at Ventura College and a member of the Ventura College Wine Lovers Group, who said that "the synergy between a great pairing makes it more than the sum of its parts; it's like two plus two doesn't equal four, it equals eight."
Sometimes the wines transformed and elevated the food, sometimes vice-versa, and sometimes the two had the effect of singing in harmony. Getting people to appreciate this dove-tailing is one of the main goals of this challenge, said Lauren Belshe, administrative/sales coordinator for the Ventura County Wine Trail and organizer of the event.
With a dozen combinations involving 24 different businesses and each doing so well, it became dizzying to think about which pairing was best. Then again, the awards made for a fun context but didn't matter all that much. The value of this event is in the breadth of experience; singling out teams in a competitive format ends up feeling arbitrary. The excitement there was less about any one or two participants than it was about the sense of our county's growing bounty.
As Belshe put it: "It's really hard to just pick two winners when really everyone is a winner. It's just great to get everybody together to show what we can do in Ventura County."


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Canterbury Rare Breeds Auction and Heritage Lifestyle Day
Sunday March 12, 2017, 10am to 3pm
The Canterbury Group of the Rare Breeds Conservation Society of New Zealand Inc is holding its annual Rare Breeds Auction. View hundreds of lots of heritage poultry that are up for auction plus sheep, cattle, rabbits and a rabbit hopping display, stalls, food, baby chickens and all the attractions Willowbank has to offer at a reduced entry fee. See www.rarebreeds.co.nz for more details.
Willowbank Wildlife Reserve, Hussey Road, Christchurch, adult entry $10, child entry $5 between 10am and 1pm
There is still time to enter poultry for auction. If you are interested - entries close on February 23rd. It's a lovely day out for the whole family and an excellent opportunity to buy and sell pure bred poultry and rare breeds of livestock. It's all on display so no surprises!


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D Man
Diner's Night Suit In D-Blue SKU: FAC0463-D-BLUE
Rs.2,740.00 PKRFAC0463-D-BLUE
Diner's provide free shipping on all cash on delivery orders. There are no delivery charges and you pay for your order when you receive it.
It takes 2 to 3 days after confirmation to process your order while courier delivers the parcel at your doorstep in 2 to 3 working days. As soon as we ship your order a tracking number is provided you to track your shipment. Courier service rider makes 2 attempts to deliver your parcel.


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The Bavarian National Museum hosts different important collections. An art historical collection and a folklore collection. The building that hosts the exhibitions has been opened in the year 1900. Nowadays it has several branches all over Bavaria, that host special collections.
Munich is the capital of the German state of Bavaria and is Germany's third largest city, located on the River Isar north of the Bavarian Alps. Black and gold - the colors of the Holy Roman Empire - have been the city's official colors since the time of Louis IV, Holy Roman Emperor.


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Our goal is to start your project and complete it as quickly as possible. We don't want you inconvenienced longer than necessary. When we say we'll be there, we're there. We handle everything from concept to completion.
There is no better way to enjoy the beautiful Florida weather than dining and entertaining with friends and family outdoors.
If you love entertaining friends and family, add value to your home, or maybe you just want a way to spend more quality time with your family? And who doesn't just love a good BBQ, right?
An outdoor kitchen is probably what you've been waiting for! They can easily be adapted to small spaces, will add value to your home for years to come, and let you enjoy your home like you've always wanted!
1) Do you sell me an outdoor kitchen and then sub out all the work?
From design to installation, he handle everything in-house. For over 18 years, we've been building outdoor kitchens in the Central Florida area, and use our experience to make sure your family gets the most out of their outdoor living space.
2) What materials do you use?We use a welded, galvanized steel frame for the highest durability and structural strength. This also allows us to maximize the interior cabinet space, and accommodate many more amenities when space is limited.
3) Will the interior of my cabinetry be finished?
The inside of our outdoor kitchens are as beautiful as the outside. We finish the interior of all cabinetry. There are no hanging wires, brackets, screws, or unfinished surfaces unfit for storage.
4) Are you licensed, reputable, and reliable?
We are a licensed contractor (# CGC1518095) in the State of Florida. We handle all electrical, gas lines, and water lines you may need for outdoor sinks, fire pits/torches, and landscape lighting. We have a great track record and have serviced Central Florida for over 18 years. We have references available and a vast portfolio of outdoor living, including putting greens, infinity pools, summer kitchens, lanais, and more. We handle everything from concept to completion.
Our Company's Focus is absolute diversity in all aspects of remodeling. We manage all aspects of the project's time schedules because we handle and complete all remodeling tasks ourselves. We have a proven track record and ongoing training with the newest construction materials. We can design together with our clients or work to improve the clients existing architectural prints. From concept to completion.


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Hello everyone! Just two more days until the kick-off to our fabulous Spring Garden Party. We have a full week of festivities and fun planned to help celebrate the arrival of our favorite season here at Garden Supply. Each day, locally owned businesses from our community will gather here to share. Come "garden" with our local growers and enjoy free pizza from our wood-fire oven and hot dogs off the grill. There's something new to do each day. Be sure to check our calendar for a complete list of what's in store for you and your whole family! Prepare to be WOWed by all the amazing plants, trees and shrubs arriving daily down at the garden center. Spring is in full swing and you will not want to miss it! And in special honor of our Spring Garden Party, how about a little sale?? From Saturday, March 13th through Saturday, March 20th, you will receive 15% OFF your entire tree and shrub purchase. And have we got some beauties for you to choose from! Take a look at some of our new arrivals.
Weeping Cherries: Mahogany bark Snow Fountain Cherry
Weeping cherry trees must be included in any Top 10 list of weeping trees, since they are spectacular blooming specimens for the spring landscape. Snow Fountains blooms best in full sun and well-drained soil. A slow-growing ornamental, the branches of this weeping cherry cascade right down to the ground.
Winter Honeysuckle:
Winter honeysuckle is a delightful shrub for extending the seasons: its winter flowers bring spring to the garden several months early while, at the other end of the year, its foliage lasts well into winter.
Pussy Willows are a pure treat for spring-starved eyes. Who doesn't love to prune off a few branches studded with furry pussy willow to be brought indoors and honored as spring's earliest harbingers?
And if you're looking for a true showpiece for the winter landscape, check out these amazing Harry Lauder's Walking Sticks. Often referred to by their common names, corkscrew filbert and contorted hazelnut, these flowering shrubs make showy ornamentals prized for their twisted, architectural forms.
These are just a small sampling of what's in store for you down at Garden Supply. You truly have to see it to believe it! Stop by and pay us a visit and get in on our special 15% off all trees and shrubs sale beginning Saturday. Keep your eyes out for pink flamingos around the nursery, too. They will be marking other sales that are changing daily.
Thanks for stopping by everyone! Hope to see you all soon!


text: Sub section navigation links Administrative Rules Drafting Manual Administrative Code Indiana Register List of Agencies with Emergency Rulemaking Authority Administrative Rules Drafting Manual Download the PDF document. To view this document, please install a suitable PDF viewer. The document is not available in the context of this page. It might be accessible at the following URL: URL × Internet Explorer Support Some features of the document viewer are not currently functional in Internet Explorer. You may experience difficulty with text selection and copy/paste. To resolve this issue, you can download this document by selecting Download PDF from the More menu. We are currently working on a resolution to these issues.


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Your Taxes: What If You Owe the IRS?
Jonnelle Marte discusses the pros and cons of setting up a payment plan, paying by credit card, or just paying off taxes when you can. Photo: Getty Images.
This transcript has been automatically generated and may not be 100% accurate.
... I ... I'm all right ... well he he he the deadline ... no one ... it is already William Bush bubble ... we get in if you miss all ... to Nomar case here with this place and we are here as we are reminded appetites and hope to Texas ... by the end of my taxes yet I am ... I how much trouble again in the final meet the deadline well you might know by now that new money making idea probably no fewer than owe taxes rise and big thing is that if you don't have the money to campus and April fifteen ... I they are different ways for you ... I learned that more time ok you consider the talent ... yes ... so the sunny as most people will be able to set up a payment plan ... and this is a good option because it does reduce some of the late payment penalties ... on there's a one-time fee of one hundred and five dollars ... you can cut that and I didn't have the money to counter the out of your bank account ... on by ... are you still have to be interred for every mind that you are ... you have this outstanding balance ... I'm the thing is to try to pay with your credit card ... on TV in Honolulu ... the solution ... it depends on and on the pier stand but ... you'll need to step into priests and convenience the roughly two percent ... and then of course if you have a high in truth I type in your credit card ... this may not be the best option for you ... plus they only need thirty days or so anything that this is the noun that they can pay now ... you are going to get money from Uncle Sam and he owes you ... do they care if you ever filed ... the studio while the union leader and have opted to me years to file their returns ... any feud and again ... the refunds ... and you don't have to pay some of the early filing penalties ... and clearly I knew it and that's what's happening now is you have until April fifteen to claim any refund from two thousand and nine ... interesting yes this is really a billion dollars in unclaimed refunds for the person who is due to fall I going to bed because it is yours is the fact that people miss that deadline ... using numbers alone time that people who miss payments and the fact clear but I know that there's no the rainfall are not paying your taxes on time even if you I and and that the government actually meet you waiving late payment penalties ... for anybody who require any of the forms that were held up this year ... because of the risk of Christie online ads ... on my interest is still doing so he is known ... leave me and that yet I'd to know Mark A think you're a much


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ALVAC-HIV vCP1452 Alone and Combined With MN rgp120
|The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.
|ClinicalTrials.gov Identifier: NCT00011037
Recruitment Status : Completed
First Posted : August 31, 2001
Last Update Posted : May 21, 2012
- Study Details
- Tabular View
- No Results Posted
- Disclaimer
- How to Read a Study Record
The purpose of this study is to test how the body's immune system responds to the vaccine ALVAC-HIV vCP1452 and to determine if the vaccine is safe when given alone and with MN rgp120.
HIV infection and AIDS have no cure, in spite of recent advances in anti-HIV drugs. Many worldwide populations cannot afford the antiviral treatments for infected people. HIV vaccines offer hope for disease prevention. In this trial, 2 experimental HIV vaccines called ALVAC vCP1452 and MN rgp120 will be given to volunteers in Haiti, Brazil, Peru, and Trinidad and Tobago. The study will determine how volunteers' immune systems respond to the vaccines. (This protocol has been changed by adding new international sites.)
|Condition or disease
|HIV Infections HIV Seronegativity
|Biological: ALVAC(2)120(B,MN)GNP (vCP1452) Biological: MN rgp120/HIV-1 Biological: ALVAC(2)120(B,MN)GNP (vCP1452) placebo Biological: MN rgp120/HIV-1 placebo
|Phase 2
There is no cure for HIV infection or AIDS in spite of recent advances in antiviral therapy. Furthermore, drug therapy is too expensive for most affected populations. For this reason, there is a commitment to the development of safe, effective vaccines to prevent HIV infection and AIDS worldwide. This study evaluates the immunogenicity and safety of candidate HIV-1 vaccines, based on the canarypox vector termed ALVAC, alone and combined with an MN rgp120 product, at 3 [AS PER AMENDMENT 7/19/01: 5] international sites.
[AS PER AMENDMENT 7/19/01: Volunteers in Rio de Janeiro, Haiti, and Trinidad and Tobago comprise Part I; volunteers in Sao Paulo and Peru comprise Part II.] Volunteers from Haiti, Brazil, [AS PER AMENDMENT 7/19/01: Peru] and Trinidad and Tobago are enrolled into 1 of 3 arms and followed for 18 months. Arm 1 volunteers receive ALVAC-HIV vCP1452 at 0, 1, 3, and 6 months. Arm 2 volunteers receive ALVAC-HIV vCP1452 on the same schedule as Arm 1 and receive HIV-1 MN rgp120 subunit simultaneously with the 3-month and 6-month vaccine doses. Arm 3 volunteers receive a placebo. Blood and urine samples are collected for immunologic assays, virologic determinations, pregnancy testing, and safety assessments. Risk behavior and social harms are assessed every 6 months during follow-up [AS PER AMENDMENT 7/19/01: Social harms are assessed every 3 months during follow-up and risk behavior every 6 months]. At all clinic visits volunteers receive counseling on avoidance of HIV infection and pregnancy. Participants are tested for HIV-1 every 3 [AS PER AMENDMENT 7/19/01: the following text has been deleted: to 6] months. Counseling and follow-up for any needed medical care are provided.
|Study Type :
|Interventional (Clinical Trial)
|Actual Enrollment :
|160 participants
|Intervention Model:
|Parallel Assignment
|Double (Participant, Care Provider)
|Primary Purpose:
|Official Title:
|A Multisite Phase II Clinical Trial to Evaluate the Immunogenicity and Safety of ALVAC-HIV vCP1452 Alone and Combined With MN rgp120
|Actual Study Completion Date :
|July 2004
Experimental: 1
Participants will receive ALVAC-HIV vCP1452 at 0, 1, 3, and 6 months and MN rgp120 and Months 3 and 6
Biological: ALVAC(2)120(B,MN)GNP (vCP1452)
1 x 10^ 7 TCID50 administered intramuscularly
Biological: MN rgp120/HIV-1
0.6 mg administered intramuscularly
Experimental: 2
Participants will receive ALVAC-HIV vCP1452 at 0, 1, 3, and 6 months and MN rgp120 placebo and Months 3 and 6
Biological: ALVAC(2)120(B,MN)GNP (vCP1452)
1 x 10^ 7 TCID50 administered intramuscularly
Biological: MN rgp120/HIV-1 placebo
Alum placebo administered intramuscularly
Placebo Comparator: 3
Participants will receive ALVAC-HIV vCP1452 placebo at 0, 1, 3, and 6 months and MN rgp120 placebo and Months 3 and 6
Biological: ALVAC(2)120(B,MN)GNP (vCP1452) placebo
ALVAC placebo administered intramuscularly
Biological: MN rgp120/HIV-1 placebo
Alum placebo administered intramuscularly
- Immunogenicity and safety of two vaccine regimens [ Time Frame: Throughout study ]
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies.
|Ages Eligible for Study:
|18 Years to 60 Years (Adult)
|Sexes Eligible for Study:
|Accepts Healthy Volunteers:
Inclusion Criteria
Volunteers may be eligible for this study if they:
- Are from Brazil (Rio de Janeiro), Haiti, Peru, or Trinidad and Tobago.
- Are 18 to 60 years old.
- Are HIV-negative.
- Have not developed a sexually transmitted disease in the last 6 months.
- Have had no more than 2 sexual partners in the last 6 months.
- Have not injected drugs or used crack cocaine in the last 6 months.
- Have not exchanged sex for money or drugs in the last 6 months.
- Have used an adequate birth control method for 1 month prior to study injections and intend to continue for the injection period (7 months).
- Are available for 18 months of follow-up.
- Have a normal history and physical examination.
- (The criteria for inclusion have been changed from the original.)
Exclusion Criteria
Volunteers will not be eligible for this study if they:
- Have had immune diseases, chronic illness, or cancer (unless cured by surgical removal), or have used medications affecting the immune system.
- Have a medical or mental condition, or job that interferes with the study requirements.
- Have a sexual partner who is HIV-infected, unless practicing abstinence or have always used condoms for the last 6 months.
- Have a sexual partner at high risk of HIV infection.
- Have received live vaccines or experimental agents within 30 days prior to planned vaccination.
- Have received blood products or immunoglobulin in the past 6 months.
- Have active tuberculosis.
- Have had a severe allergic reaction, including 1 requiring hospitalization or medical care, or a serious reaction to vaccines.
- Have received HIV-1 vaccines or placebo in a previous HIV vaccine trial.
- Are pregnant or breast-feeding.
- Are allergic to egg products, thimerosal, or neomycin.
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT00011037
|Projeto Praça Onze/Hesfa Crs
|Rio de Janeiro, Brazil
|Les Centres GHESKIO CRS
|Port-au-Prince, Haiti
|Barranco CRS
|Lima, Peru
|Trinidad and Tobago
|CCPRI Med. Ctr. - Med. Research Foundation of Trinidad & Tobago
|Port of Spain, Trinidad and Tobago
|Study Chair:
|Peter Wright
|Responsible Party:
|National Institute of Allergy and Infectious Diseases (NIAID)
|ClinicalTrials.gov Identifier:
|NCT00011037 History of Changes
|Other Study ID Numbers:
10529 ( Registry Identifier: DAIDS ES Registry Number )
|First Posted:
|August 31, 2001 Key Record Dates
|Last Update Posted:
|May 21, 2012
|Last Verified:
|May 2012
HIV Antibodies
HIV Antigens
HIV Envelope Protein gp120
AIDS Vaccines
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Neutralization Tests
Cytotoxicity, Immunologic
Viral Proteins
Trinidad and Tobago
HIV Preventive Vaccine
HIV Seronegativity
HIV Infections
Lentivirus Infections
Retroviridae Infections
RNA Virus Infections
Virus Diseases
Sexually Transmitted Diseases, Viral
Sexually Transmitted Diseases
Immunologic Deficiency Syndromes
Immune System Diseases
Immunologic Factors
Physiological Effects of Drugs


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Marketing Spotlight: Crosspointe Church
Making sure people can find your church in the search engines is important for any church, but it can be difficult if your church isn't located close to the center of town, you have a new website, or the church just created it's listings in Google and Bing. So, how do you overcome such obstacles?
Crosspointe Church:
I remember meeting Dr. Greg Crocker, pastor of Crosspointe Church. Crosspointe is a Christian church in Sanger, California with its campus located in the grapevines of Fresno County. Their mission is "Connecting people to God and to each other". The members of Crosspointe seek to accomplish this mission both through personal ministry and by having a strong online presence. I've been honored to be able to work with Dr. Crocker and Crosspointe to achieve their mission online.
I asked Dr. Crocker to share with us why they decided to do search engine optimization (SEO) for their church's website and what their experience has been with it. Here's what he had to say:
"It is difficult to find a quality SEO team for churches. You can find people that do SEO, and people that understand church, but very rarely a thorough knowledge of both. Very few secular companies understand the terminology of the Christian world – you can end up with all kinds of weird and even malicious link building, on-page SEO, and more. We were so glad to find Kurt and the team at OurChurch.com because we've had numerous bad experiences with other companies (some of which were quite large).
One SEO company actually worsened our positions in the search engines – we think it may have been sabotage, but were at least able to cut loose from them, and I believe Kurt and the team at OurChurch.com repaired the damage they had done, and we are now back on track.
OCC's quality SEO technicians have a thorough understanding of the area they are working in – in this case, churches. OurChurch.com is run by solid Christian people that not only attend various churches in the area, but also work with many churches around the U.S. They understand exactly what churches need to do with their SEO.
Why do churches need SEO?
The internet is becoming the next phone book – people go online to look for a new church, and a good SEO team can get you on the first page of local results – kind of like getting a better ad spot in a phone book. And I can tell you that the top three spots get the most clicks – and it takes good SEO to get there. OurChurch.com can get you to those prime positions."
Targeting Fresno:
Crosspointe has members who live in Fresno, CA and they wanted to reach out to Frenso, but the church is located in Sanger, CA, a city adjacent to lager city of Fresno. One of the ways Crosspointe chose to overcome the obstacle of not being at the center of one of the cities they wanted to reach was through citations.
Citations are listings or mentions of the church with their contact information. Citations often provide links and they serve to reinforce for the search engines both that the church does exist and that the church is relatively popular (it's being mentioned a lot around the web). By getting citations for Crosspointe Church we were able to regain search rankings they had lost previously and start to rank in the top 10 for Fresno related keywords, like "Churches in Fresno" and "Fresno churches".
What's in a Name?
The folks at Crosspointe are a bold Christian congregation. While the church has a long and rich heritage, they decided they could better achieve their mission of connecting people to God and to each other by making some changes in the church, including changing their name. This kind of change usually carries with it some challenges within the congregation and the community, but there was one other challenge they soon discovered…online.
Around the web there were dozens and dozens listings and references to the church under it's old name. This was not only confusing for people searching for a church, but actually hurt the church in the search engines. So, one of the tasks we took on was changing the various listings of the church under their old name to the new name. This was a team effort. OurChurch.Com was able to find and change many of the listings and Greg and his congregation were able to find some and were very helpful in verifying the church information for the sites.
This is something many organizations don't realize, but your contact information is out there on the Internet to some extent, even if you aren't trying to put it out there. So, if you change your name, location, phone number, or web address, you need to go around and find the various places online that are listing the old contact info and get it changed to the new information.
If you happen to be in the Sanger/Frenso, CA area, be sure to visit Crosspointe Church.
Your Turn:
I hope you will consider how SEO and citations can help your church. We'd love to talk to you about it. Request a free, no obligation consultation today!
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Listen: Dr. John's Locked Down
Locked Down
Dr. John
(Nonesuch Records, 2012)
In the deranged days of 1968, New Orleans musician Mac Rebennack decided to join the fun by dubbing himself Dr. John the Night Tripper, taking to the stage in a Mardi Gras Indian-inspired costume and making music thoroughly marinated in his hometown's Afro-Caribbean musical traditions and religious lore.
In the decades that followed, Dr. John stayed that musical course, and even had a few pop hits along the way. On Locked Down, Dr. John, now 71, has submitted himself to the production guidance of 30-year-old Dan Auerbach, guitarist-singer of The Black Keys. The result is an album that starts from New Orleans but ventures up-river to Memphis and even back across the waters to Mother Africa.
Sonically, the album is all of an Afro-pop piece. There are percussive guitar riffs, punctuating blasts of horns, and layers of polyrhythmic percussion, all held together by a slippery bass guitar. Even the keyboard is played as a percussion instrument, with Dr. John abandoning the New Orleans stride piano style that has dominated his recent work for choppy bursts of Farfisa organ.
Dr. John continues his recent penchant for political lyrics here. One song asks the musical question, "Rebellion, revolution, is it the final solution?" while another claims that the "KKK and CIA [are] all playing in the same cage."
But the record closes with unfamiliar notes from the old Night Tripper—humility and gratitude. There's a public apology to the artist's neglected children and grandchildren. A closing track, "God's Sure Good," proclaims, "He taught me how to live and let live. I'm so thankful; the breath of life he gives. . . . I listen to his still small voice; I believe I've made the perfect choice." And by then the listener is grateful, too, for Mac Rebennack and for this album.
This article appeared in the June 2012 issue of U.S. Catholic (Vol. 77, No. 6, page 42).


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Baguio Travel Information
The city of pines, native woodwork, wagwagan bargains, and the freshest fruits and vegetables.
High up in the northern part of Luzon lies Baguio, a city nestled within the mountain range in the province of Benguet. Dubbed the Summer Capital of the Philippines, the cool climate in the mountain city attracts visitors especially during the hottest months of the year. Baguio is also one of the most popular tourist spots in the country.
Because of its location up in the mountains, what is now known as Baguio City remained barely touched by Westernization until the American period.
- Before Baguio became the city it is today, it was an Ibaloi town by the name of Kafagway.
- When Americans arrived in the 1900s, they built Kennon Road, which connected Baguio with Pangasinan. In 1903, a residence was built in the area to accommodate the American governor-general during the hot summer months. Parks and structures were built soon after, and in July of 1903, the Americans dubbed Baguio as the Summer Capital of the Philippines.
- By 1904, American architect Daniel Burnham had laid out a plan for the city. Baguio was declared a city in September 1, 1909.
- In 1945, General General Tomoyuki Yamashita and Vice Admiral Okochi surrendered to American authorities in Camp John Hay.
- A massive earthquake in 1990 destroyed a large part of Baguio, but the city was rebuilt with aid from the national government and international donors.
Travelers' Attractions
Baguio City has all sorts of activities and sights for a wide range of travelers:
- Those looking to relax can take a stroll around the city or in one of the city's many parks. There are also plenty of interesting shops and museums, as well as several spas in the city.
- Adventure seekers and sporty types will find plenty to do in Baguio, such as trekking up the mountainside and exploring the mines, or going horseback riding.
- Those interested in history can visit some of Baguio's old buildings and American-era institutions, while those interested in local culture and traditions can go to the Tam-Awan artists' village.
Getting There and Away
There are several bus lines that travel from Manila to Baguio, passing through provinces in Central Luzon and the Ilocos Region. Some of these bus services include Dagupan Bus, Dangwa, Genesis, Greenland, Partas, Philippine Rabbit, Saulog Transit, Tranco, and Victory Liner.
Go to list of Baguio City resorts and hotels
Baguio City


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AT&T's Parent's Text Tutorial offers the following tips to text messaging. Since each message can only contain up to 160 characters, users have developed a short-hand system to allow them to say more in one message. One outcome of this practice is that punctuation is widely disregarded. Below, are more basic tips:
- Take out vowels. For example, shorten 'Text' to 'TXT'
- Replace words with symbols and numbers — For example, instead of writing the word 'FOR,' use the word '4.' So when the letters 'FOR' apppear as part of another word like 'FORGIVE,' it's usually texted as '4GIVE.' More examples include:
People = PPL
Please = PLZ Video: Text messaging costs Because = BCUZ
- Text messaging is also highly phonetic. So instead of saying 'YOU,' you might just say, 'U.'
- Combine all the methods above. An example of this would be the word 'ANYONE.' By using the method of sounds and the method of replacing words with symbols and numbers, the word 'ANYONE' becomes 'NE1.'


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My cart
$ 0.00
What's the biggest complaint that everyone makes about eyeglasses? The number one gripe is that they don't fit just right. Well, Sferoflex eyeglasses are here to change that forever. Crafted by Luxottica, the world's leader in fashionable eyewear, Sferoflex frames are named after their revolutionary flex temples that allow them to fit anyone perfectly. Slide a pair on and you'll notice the difference immediately. Not only does the fit feel right, but the unique hinges also create such a comfortable feel that you'll hardly know you're wearing them. No more discomfort; no more adjusting. Of course, the technology is great, but these wouldn't be stellar eyeglasses without a dash of style. Luckily, Sferoflex frames are on trend with styles that are designed to go with any look.


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Top Items:
New York Times:
President Obama Misses a Chance on Foreign Affairs — President Obama and his aides heralded his commencement speech at the United States Military Academy at West Point on Wednesday as a big moment, when he would lay out his foreign policy vision for the remainder of his term and refute his critics.
Ed Morrissey / Hot Air: WaPo, NYT, WSJ agree: Obama speech "ludicrous ... uninspiring ... disturbing"
Michael Crowley / TIME: Obama's Foreign Policy Speech Sounds Familiar
Jonathan Topaz / Politico: Edit pages pan Obama speech
Scared Monkeys: Former VP Dick Cheney Says Barack Obama Is a "Very, Very Weak President, Maybe the Weakest Certainly …
Doug Mataconis / Outside the Beltway: Obama's Cloudy Foreign Policy Vision
Kyle Becker / Independent Journal Review: 9 Media Pubs Slam Obama's Speech at West Point: 'Icy,' Ludicrous,' 'Uninspiring' & 'Disturbing'
Doyle McManus / Los Angeles Times: Opinion What Obama didn't explain in his foreign policy speech at West Point
David Hudson / White House.gov Blog: "America Must Always Lead": President Obama Addresses West Point Graduates
Brent Baker / NewsBusters: Brian Williams Asserts Obama 'Called for a Muscular, If Not Militaristic, Foreign Policy'
Tom Maguire / JustOneMinute: I Love The Smell Of Burning Straw In The Morning
Justin Sink / The Hill:
Cheney: Obama 'weakest' president of my lifetime — Former Vice President Dick Cheney blasted President Obama as the weakest president of his lifetime on Wednesday, following the president's speech defending his foreign policy approach at the West Point commencement ceremony.
Discussion: Washington Monthly, Politico, Daily Kos, No More Mister Nice Blog, Hinterland Gazette and The Huffington Post
Martin Longman / Washington Monthly: Is That Horrible Man Speaking Again?
Kendall Breitman / Politico: Dick Cheney: President Obama 'very weak'
Hunter / Daily Kos: Dick Cheney, the architect of America's biggest foreign policy blunders, calls Obama 'weak'
Steve M. / No More Mister Nice Blog: DICK CHENEY IS BASICALLY JUST TODD KINCANNON WITH PHONY GRAVITAS
Janet Shan / Hinterland Gazette: Dick Cheney: Barack Obama Maybe the Weakest President in My Lifetime …
Chris Gentilviso / The Huffington Post: Once Again, Dick Cheney Thinks Obama Is 'Weak'
Nile Gardiner / The Hill:
Obama's empty foreign policy vision falls flat at West Point
Obama's empty foreign policy vision falls flat at West Point
Discussion: CNN
Kevin Liptak / CNN: Obama revamps his foreign policy baseball metaphor
Kiersten Nuñez / FOX13Now.com:
Students at Utah school upset to discover yearbook photos were altered before publication — WASATCH COUNTY, Utah — Female students at a Utah high school want to know why their yearbook photos were altered to show less skin without them knowing about it. — The students who were surprised …
Discussion: ThinkProgress, JONATHAN TURLEY, CBS Las Vegas, The Daily Caller, MyFOX8.com, The Week, KTXL FOX40, I Acknowledge, Jezebel, Gawker, WREG-TV and The Raw Story
Eric Owens / The Daily Caller: Utah High School Randomly Modifies Girls' Yearbook Photos To Remove Cleavage, Bra Straps, Tats
Jordan Valinsky / The Week: Students outraged over high-school yearbook's photoshopping for modesty
Jameson Parker / I Acknowledge: Utah School Caught Photoshopping More Clothes Onto Female Students' Yearbook Pictures
Gabrielle Bluestone / Gawker: Utah High School Photoshopped Students to Make Yearbooks Less Sexy
Ashley Crockett / WREG-TV: Students upset yearbook photos were altered
Andrew Kaczynski / BuzzFeed:
John Boehner: "I'm Not Qualified To Debate Science Over Climate Change" — John Boehner says he's not a scientist. — View Video › — House Speaker John Boehner said Thursday that he's "not qualified to debate the science over climate change" while slamming the Obama Administration proposed plans …
Discussion: ThinkProgress, The Huffington Post and Washington Monthly
Katie Valentine / ThinkProgress: John Boehner: 'I'm Not Qualified To Debate The Science Over Climate Change'
Kate Sheppard / The Huffington Post: John Boehner: 'I'm Not Qualified To Debate The Science Over Climate Change'
Martin Longman / Washington Monthly: This is Not Leadership
Jake Sherman / Politico:
John Boehner's friends plot tea party crackdown — They've given John Boehner constant headaches during his three years as speaker. — Now, Boehner's friends are trying to make sure that a small pocket of tea-party-aligned Republicans won't have a chance to derail his speakership next year.
Russell Berman / The Hill:
Boehner stands by Shinseki
Boehner stands by Shinseki
Discussion: Outside the Beltway and Politico
Doug Mataconis / Outside the Beltway: Political Vultures Circling Around Eric Shinseki, Deservedly So
Steve Scalise to push GOP leaders on Obamacare alternative
Steve Scalise to push GOP leaders on Obamacare alternative
Discussion: Daily Kos
Joan McCarter / Daily Kos: Repeal and replace: it's back, sort of
Katy Steinmetz / TIME:
The Transgender Tipping Point — Nearly a year after the Supreme Court legalized same-sex marriage, another social movement is poised to challenge deeply held cultural beliefs — In the beaux-arts lobby of the Nourse Theater in San Francisco, men in deep V-necks and necklaces walk by women …
Discussion: Mashable, Washington Post, Jezebel, Joe. My. God., ThinkProgress and Towleroad News #gay
Brian Anthony Hernandez / Mashable: Trans Star Laverne Cox on Time Cover: 'This Is Way Bigger Than Me'
Steven Petrow / Washington Post: Civilities: Transgender etiquette 101
Kate Dries / Jezebel: Laverne Cox Is on the Cover of Time Magazine
Joe Jervis / Joe. My. God.: This Week's TIME Magazine Cover
Andy Towle / Towleroad News #gay: Trans Actress and Activist Laverne Cox Featured on Cover of TIME Magazine: PHOTO
Katy Steinmetz / TIME:
Laverne Cox Talks to TIME About the Transgender Movement — The Orange Is the New Black star on politics, happiness and why genitalia isn't destiny — On TIME's cover this week is an unlikely icon: Laverne Cox. Bullied and harassed for appearing feminine while growing up in Mobile …
Discussion: Mediaite, Hollywood Life, LightBox and Colorlines
Amanda Michelle Steiner / Hollywood Life: Laverne Cox: 'OITNB' Actress Says Genitals & Biology Are Not Destiny
Katy Steinmetz / LightBox: Meet Transgender America
Jamilah King / Colorlines: Laverne Cox Makes History on the Cover of TIME
Annalyn Kurtz / CNNMoney.com:
U.S. economy shrinks, but it's not a big deal — Brace yourself. The U.S. economy looks like it went on a roller-coaster ride at the start of the year. — Revised numbers released Thursday show the economy shrank in the first quarter, marking the first downturn since early 2011.
Discussion: Hot Air, Rush Limbaugh, NewsBusters, Outside the Beltway and Patterico's Pontifications
Ed Morrissey / Hot Air: Boom: Q1 GDP revised downward to -1.0%
Doug Mataconis / Outside the Beltway: The Economy Actually Shrank In The First Quarter Of 2014
Wall Street Journal:
U.S. GDP contracted at 1% pace in first quarter
U.S. GDP contracted at 1% pace in first quarter
Discussion: White House.gov Blog, The Gateway Pundit, TheBlaze.com and Washington Free Beacon
Benjamin Weingarten / TheBlaze.com: The six things you're NOT supposed to do in an economic downturn (hint: we did and continue to do all of them)
Frigid winter takes toll as US GDP contracts for first time in 3 years
Frigid winter takes toll as US GDP contracts for first time in 3 years
Discussion: VodkaPundit and NPR
Stephen Green / VodkaPundit: Your Thursday Dose of Doom & Gloom
Bill Chappell / NPR: U.S. GDP Fell 1 Percent; First Drop In 3 Years
Jeanna Smialek / Bloomberg:
U.S. Economy Shrinks for First Time Since 2011; Pent Demand Suggests Temporary Setback
U.S. Economy Shrinks for First Time Since 2011; Pent Demand Suggests Temporary Setback
Discussion: Bloomberg View, BizzyBlog and Weekly Standard
Megan McArdle / Bloomberg View: U.S. Economy Takes Another Sick Day
Tom / BizzyBlog: 1Q14 Gross Domestic Product, First Revision (052914): An Annualized 1.0% Contraction
Andrew Dugan / Gallup:
Despite Enrollment Success, Healthcare Law Still Unpopular — Affordable Care Act is most popular among blacks, least among whites — WASHINGTON, D.C. — Although the Obama administration is boasting higher-than-expected enrollment for the Affordable Care Act, Americans' attitudes toward …
Discussion: The Daily Caller, The Hill, Hot Air, Mediaite, Washington Post, Heritage Action for America and The PJ Tatler
Sarah Hurtubise / The Daily Caller: Gallup: Most Think Obamacare Won't Improve Health-Care System
Rebecca Shabad / The Hill: O-Care still unpopular despite enrollment numbers, poll finds
Sean Sullivan / Washington Post: Poll: Obamacare still unpopular after enrollment surge
Katherine Rosario / Heritage Action for America: Morning Action: Obamacare is Still Unpopular According to New Poll
Bryan Preston / The PJ Tatler: Gallup: Obamacare Still Isn't Very Popular
Glenn Kessler / Washington Post:
What did Mitch McConnell mean when he suggested the Kentucky state exchange was 'unconnected' to Obamacare? — Reporter: "Should Kynect be dismantled?" — Sen. Mitch McConnell (R-Ky.): "I think that's unconnected to my comments about the overall question here."
Igor Volsky / ThinkProgress: Republicans' Suprising New Position On Obamacare
Jonathan Bernstein / Bloomberg View: McConnell's Obamacare Gaffe Deserves Its Own Category
Jed Lewison / Daily Kos: McConnell-land tries to explain why Mitch is claiming that we can repeal Obamacare and keep it too
Michael Tomasky / The Daily Beast: Mitch McConnell's Big Obamacare-Kynect Lie
Phillip M. Bailey / WFPL: Alison Lundergan Grimes Inching Towards Obamacare Defense
Ron Beasley / The Moderate Voice: Mitch McConnell, Partisan Hack And Liar
Martin Longman / Washington Monthly: McConnell Should Be Removed "Root and Branch"
Mark Tower / MLive.com:
Paul Ryan on being mistaken for Anthony Weiner: 'I don't know how this keeps happening' — MOUNT PLEASANT, MI — During his address to a group of Republicans gathered for an event at Bucks Run Golf Club Wednesday, May 28, in Isabella County, U.S. Rep. Paul Ryan's main message was that of praise …
Discussion: Politico, Talking Points Memo, Business Insider, The Moderate Voice, OnPolitics, The Huffington Post, Washington Post and Althouse
Kendall Breitman / Politico: Paul Ryan: People think I'm Anthony Weiner
Catherine Thompson / Talking Points Memo: Paul Ryan Can't Figure Out Why People Keep Mistaking Him For Anthony Weiner
Colin Campbell / Business Insider: Anthony Weiner Says Being Confused With Paul Ryan Is The 'Final Insult'
Joe Gandelman / The Moderate Voice: Paul Ryan Can't Understand Why Some People Mistake Him for Anthony Weiner
Catalina Camia / OnPolitics: Why does Paul Ryan get mistaken for Anthony Weiner?
Ashley Alman / The Huffington Post: Paul Ryan Keeps Getting Confused For Anthony Weiner For Some Reason
Eric K. Shinseki / USA Today:
VA secretary: I'm committed to restoring integrity — IG findings are reprehensible, and we are not waiting to set things straight. — The findings of the interim report of VA's Office of Inspector General on the Phoenix VA Health Care System are reprehensible to me and to this department …
Discussion: ABC News, Politico, Yahoo! News, Fox News, The Daily Caller, TheBlaze.com, Mediaite, Wall Street Journal, Real Clear Politics, ABC News, Hot Air, Washington Post, National Review, The Daily Beast, The Heritage Foundation, The Lead with Jake Tapper, OnPolitics, CNN, Daily Kos, KDVR.com, The World's Greatest …, Weekly Standard and The PJ Tatler
Erin Dooley / ABC News: Veterans Adminstration Scandal: What You Need To Know
Richard Halloran / Politico: Shinseki's Not a Quitter — When Eric. K. Shinseki was a cadet …
Ben Jacobs / Yahoo! News: Why Dems Want the V.A. Chief Gone, ASAP
Brendan Bordelon / The Daily Caller: VA Secretary Shinseki Plays Defense: 'We Are Doing All We Can'
Pete Kasperowicz / TheBlaze.com: Boehner explains why he is not ready to call for Shinseki's firing
Josh Feldman / Mediaite: Boehner, Pelosi Resist Calls (So Far) for Shinseki to Resign
Reid J. Epstein / Wall Street Journal: VA's Shinseki Defends Himself Amid Rising Calls for Him to Go
Michael Cipriano / Real Clear Politics: In Op-Ed, Shinseki Vows to Fix VA Problems
Michael Falcone / ABC News: Walls Closing In Around Shinseki
Ed Morrissey / Hot Air: White House aides say Shinseki on "probation"
Josh Siegel / The Heritage Foundation: Romney: Veterans Hospitals Need Competition, Standards (VIDEO)
Catalina Camia / OnPolitics: Democratic senators call on VA chief Shinseki to resign
Chelsea J. Carter / CNN: 1,700 vets denied doctors
Meteor Blades / Daily Kos: Inspector General's interim report confirms long delays for patients at Phoenix VA hospital
Niels Lesniewski / The World's Greatest …: In VA Scandal, More Senators Call for Shinseki Ouster, FBI Investigation (Updated)
Michael Warren / Weekly Standard: Udall Calls for Shinseki to Resign
Bryan Preston / The PJ Tatler: Report Verifies Neglect of Veterans at the VA; McCain Calls for Criminal Probe (Democrats Divided)
Dems join GOP in Eric Shinseki pile-on
Dems join GOP in Eric Shinseki pile-on
Discussion: Washington Post, Talking Points Memo, Right Wing News, Daily Kos, Hot Air, The Hugh Hewitt Show, Fox News, The Hill, New York Times and Mediaite
Caitlin MacNeal / Talking Points Memo: More Democratic Senators Call For Shinseki To Resign
Tiffiny Ruegner / Right Wing News: Growing Calls on Hill for Eric Shinseki's Resignation for Ignoring Veterans Immediate Medical Needs
Laura Clawson / Daily Kos: Some Democrats join calls for Shinseki to resign
Ed Morrissey / Hot Air: Shinseki: Now I'm committed to making the VA work after five-plus years on the job, or something …
Hugh Hewitt / The Hugh Hewitt Show: Lawyer Up, VA People. Today.
Cristina Marcos / The Hill: House votes to fund DOJ probe of VA
Steven Hayward / Power Line:
The Economy: Don't Look Now, But . . . News has just crossed the wire this morning that the economy contracted at an annual rate of 1 percent during the first quarter of this year. Many people will probably point to the brutal winter weather as a factor, though the Dept. of Commerce fingers …
Discussion: Yahoo! News, The Daily Caller and U.S. Bureau …
Daniel Gross / Yahoo! News: Did the Economy Have a Heart Attack?
Conor Friedersdorf / The Atlantic Online:
A Liberal Moderate's Critique of Snowden and Greenwald — In a review of Glenn Greenwald's No Place to Hide: Edward Snowden, the NSA, and the U.S. Surveillance State, George Packer, whose best work is superb, makes a number of dubious claims. They are typical of liberal moderates who acknowledge …
Discussion: Hullabaloo and Crooked Timber
Digby / Hullabaloo: Dick Cheney's Triumph
Neil Irwin / New York Times:
Thomas Piketty Responds to Criticism of His Data — Six days after The Financial Times launched an attack on the data behind Thomas Piketty's much-debated tome on inequality, "Capital in the Twenty-First Century," Mr. Piketty has offered his first detailed response to the newspaper's criticism.
Discussion: The Week
Ryu Spaeth / The Week: Thomas Piketty responds to the haters
Recalling own injuries, Obama calls for end to 'suck it up' culture in youth sports — (CNN) - Saying he wants to change a culture in youth sports where kids are encouraged to "suck it up" after sustaining head injuries, President Barack Obama on Thursday said it was time coaches …
Michelle Obama / New York Times:
Michelle Obama on Attempts to Roll Back Healthy Reforms — WHEN we began our Let's Move! initiative four years ago, we set one simple but ambitious goal: to end the epidemic of childhood obesity in a generation so that kids born today will grow up healthy. — To achieve this goal, we have adhered to one clear standard: what works.
Discussion: Althouse, The Daily Caller, JustOneMinute, Guardian, CNN, Bloomberg View and The Lonely Conservative
Chuck Ross / The Daily Caller: Michelle Obama: House Wants To 'Override Science' On Childhood Nutrition
Tom Maguire / JustOneMinute: Michelle Versus Science And Reality
Bill Chappell / NPR:
'Oh My Jesus!': Gun Violence Interview Gets Up-Close Example — An NPR report about guns in Chicago included an all-too-real example of violence, after a burst of gunfire erupted down the street from where NPR's David Schaper was conducting an interview Wednesday.
Discussion: The Raw Story and Poynter
David Edwards / The Raw Story: NPR's Chicago gun violence report interrupted by semi-automatic rifle fire: 'Oh my, Jesus!'
Kristen Hare / Poynter: NPR reporter sees a shooting while reporting on guns
Agence France-Presse:
Spain sentences 'Al-Qaeda's librarian' to eight years in jail — Madrid (AFP) - A court in Spain on Thursday convicted a Saudi man dubbed "Al-Qaeda's librarian" of the charge of membership in a terrorist organisation and sentenced his to eight years in jail.
Discussion: The Jawa Report
Rusty Shackleford, Ph.D. / The Jawa Report: "Al Qaeda Librarian" for Ansar Mujahideen Forum Sentenced to 9 Years in Spain
The Official Google Blog:
Getting to work on diversity at Google — We've always been reluctant to publish numbers about the diversity of our workforce at Google. We now realize we were wrong, and that it's time to be candid about the issues. Put simply, Google is not where we want to be when it comes to diversity …
Discussion: Mother Jones, Jezebel, Gawker, ThinkProgress, Daily Dot, Vermont Public Radio, Bloomberg View, Scripting News, The Verge, The Week, Gigaom and Mashable
Kate Dries / Jezebel: Google is 70 Percent Men and 61 Percent White
Nitasha Tiku / Gawker: Google Is Only 2 Percent Black
Rob Price / Daily Dot: Google's workforce overwhelmingly white, male
Elise Hu / Vermont Public Radio: Google's White Male-Heavy Staff Underlines Tech's Diversity Problem
Leonid Bershidsky / Bloomberg View: Congress Causes Google's Diversity Problem
Chris Welch / The Verge: Google is building the future, but its workforce looks a lot like an unwanted past
Meghan DeMaria / The Week: Google admits its workforce is overwhelmingly white, male
Lauren Hockenson / Gigaom: Google releases breakdown on the diversity of its workforce
Dennis Romboy / KSL-TV:
Sen. Hatch: Same-sex marriage inevitable, religious rights eroding — SALT LAKE CITY — Sen. Orrin Hatch said Wednesday that same-sex marriage seems inevitable as courts across the country rule against states that ban the practice. — At the same time, the seven-term Republican senator …
Discussion: OnPolitics, Washington Post and The Week
Catalina Camia / OnPolitics: Sen. Hatch: Gay marriage will be 'law of the land'
Sean Sullivan / Washington Post: GOP Sen. Orrin Hatch: Gay marriage will become the 'law of the land'
Jordan Valinsky / The Week: Republican senator: Same-sex marriage will soon be 'law of the land'
Washington Post:
In New Orleans, major school district closes traditional public schools for good — NEW ORLEANS — The second-graders paraded to the Dumpster in the rear parking lot, where they chucked boxes of old worksheets, notebooks and other detritus into the trash, emptying their school for good.
Ed Krayewski / Hit & Run: New Orleans Becomes An All-Charter School City
Intern / Log Cabin Republicans:
LCR National & LCR Texas Join To Denounce Exclusionary Policy of TX GOP — Washington, D.C. & Fort Worth, TX — Today Log Cabin Republicans announced that in the wake of denial of formal participation by gay Republicans at this year's Texas State Republican Convention, they would be joining …
Discussion: Talking Points Memo, Texas Politics and The Raw Story
Catherine Thompson / Talking Points Memo: Texas GOP Blocks Gay Conservatives From Having Booth At State Convention
Lauren McGaughy / Texas Politics: Log Cabin Republicans denied booth at Texas GOP Convention
Christina London / KNSD-TV:
Screens Smashed, Cords Cut inside DeMaio's Campaign Office — The vandalism came just six days before the primary election June 3 — A break-in was reported at Republican congressional candidate Carl DeMaio's San Diego campaign headquarters, just six days before the primary election.
Discussion: Politico, The Gateway Pundit, Joe. My. God. and Towleroad News #gay
Kendall Breitman / Politico: Break-in at GOP candidate's office
Jim Hoft / The Gateway Pundit: After Weeks of Harassment from Far Left Groups - Gay Republican's Office Vandalized, Computers Destroyed (Video)


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Project ALERT
Project ALERT® is a team of retired local, state and federal law enforcement professionals, located across the country, who donate their time and experience to the law enforcement community. Members are no longer sworn investigators, but they all share their abilities, knowledge and experience through the Project ALERT program.
Technical assistance in long term investigations
Project ALERT representatives provide technical assistance to law enforcement agencies regarding missing children investigations. They specialize in long term missing children cases and perform a wide range of functions including:
- Case review.
- Organization and analysis.
- Recommendation of investigative strategies.
- Coordination of meetings with law enforcement personnel to discuss additional resources.
Project ALERT representatives integrate NCMEC resources such as age progression, facial reconstruction, poster distribution, DNA protocol, search specialists and database searches into the investigations.
Biometric collection in missing and unidentified cases
Project ALERT representatives collect biometric and information about missing children and unidentified remains in an effort to help supply critical data to the National Missing and Unidentified Persons System, also known as NamUs , and National Crime Information Center databases.
Project ALERT helps law enforcement to access the most current forensic methods to help increase the likelihood of identifying missing children. Representatives meet directly with law enforcement investigators, coroners/medical examiners and family members to obtain critical descriptive information for NamUs and NCIC.
Outreach to law enforcement and the community
Project ALERT also provides outreach to law enforcement and the community by representing NCMEC at law enforcement conferences, providing law enforcement training and addressing community awareness initiatives.
Contact Project ALERT
This resource is available to assist law enforcement agencies in their efforts to resolve recent or long term missing child cases. For more information or to request Project ALERT assistance email firstname.lastname@example.com.
Become a Project ALERT representative
To be considered for the position of a Project ALERT representative download the application form below. For more information email email@example.com.
Project ALERT Application


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Rock Band Unplugged
Harmonix is finished with weekly DLC for Rock Band Unplugged
Harmonix has confirmed to Joystiq that weekly DLC for Rock Band Unplugged is finished. Apparently, it has "run through [its] planned slate" and the firm is "very pleased with the performance of the Rock Band Unplugged game on PSP, the Rock Band PSP bundle and PSPgo starter kit" which has 100 tracks through the disc […]
Rock Band Unplugged headlines
Wolfmother, Rob Zombie coming to Rock Band this week
EA's announced that your dosage of Rock Band this week will come in the form of track packs Wolfmother and Rob Zombie. Liz Phair and Morningwood make up for the rest of the Rock Band pack. While over on Rock Band: Unplugged for the PSP, you get Sonic Youth and Steely Dan. PR is after […]
America's PSPgo early adoption deal is Rock Band Unplugged demo
Americans that take the plunge with a new PSPgo handset are to be given a free Rock Band Unplugged demo along with the hardware, according to this Kotaku piece. Which is great. If it wasn't for the fact that Europeans get a free copy of GT PSP. The US offer's a bit more than a […]
Rumor: PSPgo to launch with Rock Band Unplugged voucher
Best Buy has the PSPgo listed on its website and it says that handheld will come with a voucher to download Rock Band Unplugged. You can get it, apparently, in Pearl White or Piano Black. No confirmation from Sony, but Best Buy has it listed anyway. More over on D'toid.
Rock Band Unplugged getting Radiohead and Killswitch Engage
Harmonix and MTV announced today that Radiohead and Killswitch Engage will arrive for Rock Band Unplugged July 16. Radiohead – My Iron Lung Killswitch Engage – My Curse Each one will run you $1.99 per track on the PSP system.
SRV, Dixie Chicks, R.E.M. land on Rock Band Unplugged
Harmonix and MTV announced today that R.E.M., Stevie Ray Vaughan, Dixie Chicks, and The Pixies have landed in the Rock Band Unplugged Music Store. Here's the track list, with each one running you $1.99: Dixie Chicks – Sin Wagon GLaDOS and Jonathan Coulton – Still Alive (Free) The Pixies – Here Comes Your Man R.E.M. […]
New DLC now available for Rock Band Unplugged
Rock Band Unplugged got four new tracks today. Avenged Sevenfold – Afterlife Dream Theater – Constant Motion Motley Crue – Dr. Feelgood Stone Temple Pilots – Sex Type Thing Each one is the usual $1.99 for your lovely PSP. You can get each the usual way through the Rock Band Store.
US PSN update, May 28 - Loads of add-ons, Resident Evil
Highlights from the US PS store update this week include a demo for Rock Band Unplugged, the Resident Evil: Director's Cut game for both PS3 and PSP with altered scenes and more gameplay options, and a massive bunch of LittleBigPlanet 2000AD stuff. Users can also expect the usual DLC along with videos and the like. […]
Rock Band PSP gets Euro date
EA, MTV and Harmonix have now given us the Euro date for Rock Band Unplugged on PSP. It's scheduled to hit our shores on June 19th, which is just ten days after the US release. Full track listing through the link.
Rock Band Unplugged track list revealed
Rock Band Unplugged is coming to PSP June 9, and the track list has been revealed for it. Musical eras span between the present with AFI to The Who in the 1960's. If you aren't tired of music games, and are interested in what's on it, read on below the break. Thanks, Joystiq.
PSP and PS3 versions of Rock Band will not make beautiful music together
Or any sort of music, for that matter. Speaking with 1UP, Harmonix said that the upcoming pint-sized version of Rock Band won't be able to interact with its bigger, more plastic-intensive brother. Does this mean we'll have to buy new songs twice — once on each platform? That sounds a bit like highway robbery, if […]
Rock Band PSP - impressions, shots, movies, kitchen sink
IGN's gone apeshit mental over Rock Band: Unplugged, posting a preview, some new screens and a few movies of the rhythm action staple's PSP version. It's out this June. Hit the links.
Rock Band PSP gets movie, tracklist, date
Harmonix just released a trailer for Rock Band Unplugged the franchise's PSP version. Get it after the break. An "Limited Edition Rock Band Unplugged PSP Entertainment Pack" is on the way, to include the black PSP-3000, a 4GB Memory Stick PRO Duo, and a PSN downloadable movie voucher for School of Rock. Exclusive tracks for […]
Rock Band: Unplugged PSP detailed by GameStop
GameStop has spilled the beans on Rock Band: Unplugged for PSP. Seems there'll be a single-player World Tour with 24 cities, supports downloadable and an in-game Rock Band Music Store, but no mention is made of online or multiplayer modes. No mention has been made of a peripheral along the lines of the one included […]
Rock Band Unplugged confirmed for PSP [Update]
Rock Band Unplugged will release for PSP this year, it's just been confirmed. This news just came out of Sony's Destination PlayStation event in the US. The game is expected to use Wi-Fi capabilities, and will be the first PSP game to use an in-game store for DLC. It'll be released in Europe and North […]
Rock Band Unplugged latest
Harmonix is finished with weekly DLC for Rock Band Unplugged
America's PSPgo early adoption deal is Rock Band Unplugged demo
PSP and PS3 versions of Rock Band will not make beautiful music together


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Or: How I Fought The Battle With Usability and Lost, But Received Actual Productivity as a Consolation Prize
Thanks in no small part to the wise ramblings of Merlin Mann, I'm hooked on the portability and flexibility of doing as much of my creative work as possible using simple text files stored on Dropbox. Documents I'm working on are always accessible to me wherever I go. I can even apply useful formatting to these plain text files, regardless of which writing software I'm using, with the relatively intuitive syntax of Markdown. Markdown is, in the words of its creator John Gruber:
…a text-to-HTML conversion tool for web writers. Markdown allows you to write using an easy-to-read, easy-to-write plain text format, then convert it to structurally valid XHTML (or HTML).
Apps like WriteUp on iOS and Byword on the Mac show me what my Markdown-formated text will look like in HTML. Here's this very blog post in Byword — first the raw markdown text:
And here's the HTML preview:
Because the files are plain text, there's no way to mess up their contents or formatting by opening them in an incompatible app. The value of this has been made evident to me recently as I tried to live the Apple dream and bounce some work back and forth between Apple's own Pages app on iOS and Mac. The file became bloated with unnecessary crap, formatting was removed, comments were deleted, and use of niceties such as curly quotes was inconsistent. I won't even mention the hassles of manually emailing the document back and forth between devices, since Apple is actively solving that issue with iCloud. But iCloud won't be so hot for Pages documents if the iPad version persists in stripping out critical formatting and features from documents created on the Mac.
Markdown solves all these issues by eschewing WYSIWYG and instead prioritizing something that, I've come to realize, might be far more valuable: keeping your hands moving on the keyboard.
The putzing-free fluidity with which I edit Markdown documents across devices has made me even more grumpy about the sorry state of mobile screenwriting. On iOS, I use two screenplay writing apps that support the industry-standard .FDX format: ScriptsPro and ScriptWrite. In all honesty, both have tremendous potential, and tremendous bugs. I've been skittish about using either for real work. Celtx Script, which I wrote about when it launched, is stable and lovely, but does not support FDX. Like Merlin (roughly) said in a recent episode of Back to Work, "I'm not going to row out to your island."
Out of desperation for a mobile screenwriting solution that would be as fluid and flexible as my Markdown text work, I engaged in some procrasductivity the other night and began researching ways of writing a screenplay in plain text. After much fiddling, I finally remembered that Final Draft actually has a heuristic for guessing the implied screenplay formatting in a plain text file. If you feed it something like this:
It will correctly import it as this:
So the ultimate mobile screenwriting solution may be, for the time being, your favorite among the many lovely Dropbox-based plaintext writing apps out there.
Are other screenwriters as dissatisfied with their workflow as I am? Is there a part of your workflow where you'd give up WYSIWYG and accurate pagination to just get words on the page in a way that freed you from a specific device, and specific software? Or do we expect that Final Draft, Inc.'s promised-but-delayed iPad app will be what we've been wanting? Or that one or more of the existing iPad apps will become awesome?
Screenwriting is challenging enough that I often catch myself "fiddling" (in the Merlin Mann definition: spending more time putzing with the tools than doing the work) with ways of making it more visual and intuitive. And now here I am fiddling with a way of making it less visual for the sake of more portability.
Maybe I should just shut up and write. Which is exactly what I did after I got this all figured out. I put all the toys away and wrote a six-page treatment for a feature I want to make.
I wrote it in Markdown. And yesterday, as I was out walking my dog, I got an idea for a small tweak. I sat down on a bench in the sun, pulled out my phone, and made the adjustment while it was still fresh in my mind.
on 2011-08-09 22:59 by Stu
Apparently I'm either not crazy, or not the only crazy person out there. Turns out others have experimented with tools to enable plaintext screenwriting.
- Via @dansturm: screenbundle for TextMate
- Via @erichocean: eleanor
- Via @vilcans: screenplain
All seem to behave like Final Draft in interpreting plain text into a screenplay format. Which is nice, but it's a one-way street. I'm thinking there might be a place for a plaintext screenplay format with markdown-like syntax, and a WYSIWYG desktop app that works with that format as easily as FDX.
The syntax would be necessary for special cases, such as forcing a Scene Heading element even when the first characters are not INT or EXT, simultaneous dialog, title pages, and centered text for the all-important "THE END." Markdown syntax would be used for **bold** and *italics,* but being screenwriters, we need _underlining_ too, and the ability to _**combine**_ them.
This way we could have the niceties of a dedicated desktop WYSIWYG app combined with the edit-anywhere flexibility of plaintext.
on 2012-01-28 05:34 by Stu
And now I really am crazy, because you can view the Screenplay Markdown sytnax proposal here.
on 2011-08-12 18:14 by Stu
And now there's a video.


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Verbatim Corporation has announced its new LightScribe 16x DVD-R media in 30-pack spindles. Verbatim, which already offers LightScribe CD-R and DVD+R media in 10-pack spindles, 20-pack slim cases and 30-pack spindles, will begin shipping the new LightScribe 4.7GB/120-minute DVD-R media in Q1 2006.
The company also is preparing to roll out LightScribe gold-background CDs and DVDs with a new coating that enables faster label printing, as well as color-background LightScribe CDs/DVDs in Q2. Engineering samples of the new LightScribe media are being showcased this week during CES in Las Vegas at Verbatim's Booth #36049, South Hall 4.
When Verbatim LightScribe media is combined with the LightScribe-enabled drives and software licensed by Hewlett-Packard Company (HP), consumers can use the same optical disc drive laser to burn content to the data side of the disc and create a silkscreen-quality label on the other side of the disc.
The LightScribe media coating was developed by Verbatim's parent company, Mitsubishi Kagaku Media Co., Ltd., (MKM) in collaboration with HP. As key backers of LightScribe technology, Verbatim and MKM are leveraging their expertise in optical technology to deliver the new LightScribe 16x DVD-R media, even faster label printing and more color choices.
Current LightScribe drive owners will be able to take advantage of the new media by updating their LightScribe system software. In addition to the current gold color, the new coating technology used on Verbatim's next-generation LightScribe CD and DVD media will enable the discs to be produced with vibrant new jewel-toned background colors such as yellow, green, and orange.
With its all-in-one approach to burning and labeling CDs and DVDs, LightScribe technology is being used by a growing number of home, office and mobile users. Now they can print professional-looking labels on the CD or DVD discs they create wherever they go -- without the need for a printer, ink cartridges or adhesive paper labels.
According to HP, 2.5 million LightScribe-enabled drives were shipped in the first six months following the January 2005 launch. NPD reports that LightScribe-enabled drives represented a record 18.8 percent of the US retail market sales for aftermarket PC DVD burners in October 2005. Consumers will be able to identify Verbatim LightScribe media through the LightScribe logo found on the packaging and in the hub of every disc.
Verbatim LightScribe 16x DVD-R discs and higher-speed media will be available in Q1 2006 with an estimated street price of $39.99 for a 30-pack spindle.


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Related Product Information
Immunoglobulin M (IgM) antibodies are generally the first to appear in response to initial antigen exposure. IgM is by far the physically largest antibody; it is in essence polymers of individual immunoglobulin molecules covalently linked by disulfide bonds. The common pentameric structure has a molecular mass of approximately 900 kDa.
For researchers interested in labeling IgM antibodies with fluorescent labels, their structural complexity and sensitivity to pH and other physical conditions requires modification of protocols optimized for IgG class antibodies. Here we describe a protocol for IgM labeling that has been optimized in our laboratory with our superior amine-reactive Alexa Fluor® dyes (Alexa Fluor® NHS and SDP esters).
The amine-reactive Alexa Fluor® dyes react with non-protonated aliphatic amine groups (the α-terminus and lysine ε-amino groups). IgG labeling reactions are typically performed at pH 8.3 to 9.0. However, IgM antibodies tend to denature at even slightly alkaline pH values and thus labeling must be done close to neutrality. Labeling is less efficient near neutrality and hydrolysis dominates over protein labeling. At the near neutral pH used to conjugate IgM antibodies, primarily α-amino end-termini will be labeled; lysine ε-amino groups have pKa values around 10.5 and are thus largely protonated and unreactive. For these reasons the dye:IgM molar ratio needs to be on the order of 50:1 or 100:1, rather than the 10:1 ratio generally used for IgG labeling.
The following is a modification of the standard amine reaction protocol that we have developed for labeling of IgG molecules using our amine-reactive dyes and protein labeling kits.
- Prepare your antibody in reaction buffer: 0.1 M sodium phosphate, pH 7.2 to 7.5, with 150 mM NaCl for improved antibody solubility (PBS is ideal). Note: Antibodies supplied in PBS, pH 7.4, with 0.1% (or less) sodium azide can be used directly in the labeling reaction. Buffers containing primary amines (e.g., Tris or glycine) will strongly interfere because they react with the NHS-ester moiety. If your IgM molecule is suspended in such a buffer it must first be dialyzed against reaction buffer.
- Transfer 100-500 μL of antibody (0.5 to 5 mg/mL) to the vial containing the dye. Mix well and incubate at room temperature for 1 hour. As the Alexa Fluor® amine reactive dyes are relatively hydrophilic (especially the SDP ester), it is not necessary to dissolve the dye in organic solvent.
Alternatively, dissolve 1 mg reactive dye in 10 to 100 uL anhydrous DMSO. This corresponds to a concentration of 100 and 10 mg/mL, respectively.
- Remove un-conjugated dye by passing the solution over a short gel filtration column (e.g. BioSpin #732-6008, BioRad). Alternatively dialysis at 2-6 °C for 12 to 24 h with four buffer changes can be used.
- Store the labeled antibody protected from light at 2-6 °C. We generally do not recommend frozen storage of antibody conjugates; if you wish to store your labeled antibody at -20 °C, we suggest adding 50% (v/v) glycerol to single use aliquots.
- For storage at 2-6 °C longer than a few weeks we recommend adding sodium azide (NaN3) to a final concentration of 0.02-0.05% (w/v) (0.3-0.75 mM), which will inhibit microbial growth
In all labeling reactions we recommend testing more than one ratio to achieve optimal degree of labeling (DOL).
Please see our website for how to determine the DOL and additional tips on labeling and reactive reagent choices
For Research Use Only. Not for use in diagnostic procedures.


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The Shell Graduate Programme
In the Corporate Functions area of the Shell Graduate Programme, you'll experience a three-year training programme that includes two 18-month job rotations. From day one, you'll benefit from being given real responsibility with our highly experienced IT teams, working alongside major global corporations such as Microsoft, SAP and IBM. We seek innovators and visionaries who can work alongside our leading IT experts to push the boundaries of Shell's operations. You could gain experience on groundbreaking projects while exploring your creativity and developing your leadership skills.


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I have managed to snap a couple of lunch pictures here and there. Nothing to write home about, or rather, blog about, but to prove my children are not being starved during these dog days, here's a peek at what's been happening. :)
A couple super quick and easy lunches in Lock and Lock "4 square" boxes:
These were identical meals containing:
- a blueberry bagel thin with organic cream cheese, cut in quarters (each box had two sections)
- a couple yummy organic strawberries
- pretzels
- cucumber slices
Snacks on a pool-bound afternoon:
- blue box: Goldfish crackers and organic strawberries (Ethan's)
- pink box: Goldfish crackers and blueberries (Paige's)
These boxes are from a company called Sistema Klip-It. I found these at Old Navy of all places! :)
And some easy peasy Laptop Lunches for my two littlest tikes:
- peanut butter and fruit preserve half sandwich on multigrain
- cucumber slices with hummus (sandwiched between the slices)
- Pirate Booty
- I removed one box to make space for a juice box and Babybell cheese
And Paige's:
- the other half of the peanut butter and fruit preserve sandwich
- same cucumber and hummus bites
- Pirate Booty
- Lemonade box with Babybell cheese
Both of their sandwiches are topped with organic fruit leather than I cut using cute bento cutters. Tyler's is supposed to be a firetruck, but the fruit leather was really too firm for the details to show through. So he ended up with more of a funky rectangle.... Paige's cute bear face worked well, but there isn't much contrast between the dark fruit leather and the dark bread for it to show very well in the photo.
I can't tell you how excited I'll be to be posting lunches one a regular schedule! Once the kids are in school I should have a nice routine again. Paige will be in Kindergarten this year, full-day here, so that will be an extra daily lunch. Be excited! :p
Hope everyone is having a super summer!!!
(the top two photos are pulled from my Instagram feed, that's why they look a little goofy. I'm Invisimom on Instagram, I don't often post foody photos, mostly kid and what-I'm-up-to daily random stuff, but I'm happy to have more Insta-friends!. I'm madly in love with that app!)


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Notifications and indicators
Application notification icons appear in the top-left corner of the home screen. These notifications inform users about application-specific events, such as a new email. You can trigger an application notification when a new event or new information is available that users might want to know about.
Status indicators appear in the top-right corner of the screen. These indicators display status information that applies across applications, such as battery power level and wireless connection.
When an application is running, users can view the notifications and indicators by swiping from the top-left or top-right toward the center of the screen. Users can access the details of a notification by tapping the icon at the top of the screen.
Best practices
- Use application notifications judiciously. Try to determine the types of events that users want to be notified of. Test your notification strategy with users to avoid unnecessary distractions or interruptions.
- Do not use application notifications to provide feedback to users, such as providing confirmation of a specific user action.
- Avoid using ellipses (...) to indicate progress. Ellipses should be used only to indicate truncated text. Instead, provide other visual indicators, such as activity or progress indicators.
Got questions about leaving a comment? Get answers from our Disqus FAQ.comments powered by Disqus


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Loading some great jobs for you...
Home Care Assistance is currently hiring caregivers, CNA/CHHA can work WEEKENDS and HOLIDAYS. You can work as little as 20 hours a week but long hours and full time are preferred long term.We treat our caregivers as valued employees and are looking for dedicated, responsible and passionate individuals to join our team. Starting hourly rates ranges from $13.50 up to $15, depending on location and case assignments. Our company offers benefits like Health, Dental and Vision Insurance and 401K.
Our Service Area includes: Oakland, Alameda, Richmond, Castro Valley, El Cerrito, San Pablo and many more locations!
Responsibilities may include:
Cooking, light housekeeping, laundry and changing bed linens
Bathing, dressing and grooming assistance
Transportation to doctor appointments, supermarket, and pharmacy
Assisting with walking and transfers from bed to wheelchair
Medication reminders
Launch your career - Upload your resume now!Upload your resume
Loading some great jobs for you...


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Nice work! Enjoy the show!
Link Copied!
U.S. to demand U.N. keep sanctions on Pyongyang
Opening Sequence
Giuliani paid $500K by indicted associate's firm
U.N. urges Turkey to investigate Syria executions
Hunter Biden defends overseas work, expresses regret
LeBron says Morey 'wasn't educated' on Hong Kong
Slow progress for Notre-Dame reconstruction, six months after blaze
'Totally irresponsible' Lam slams U.S. senators
Facebook e-coin presses on despite defections
Atwood, Evaristo take literature's Booker Prize
Closing Sequence


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Artist's description:
Thank you for looking at this large painting from the Dee Brown.
The painting is painted using high quality acrylics on quality cotton canvas and is varnished, hand-signed on the back and ready to hang before we send it to it's destination.
A signed Certificate Of Authenticity will be included with the artwork as well.
Dee Brown paintings distingiuse themself from other paintings by using earthtone colors combined with raw used structures which gives it a warm and at the same time industrial look.
The size of this particular artwork:
Height :39,4 inches (100 cm)
Width : 39,4 inches (100 cm)
Depth : 1,5 inches (4 cm)
The 3d sides are always painted as part of the painting, so there is no need for framing.
All paintings are very carefully packed in cardboard boxes with layers of bubble wrap to protect them to ensure the painting will reach you safely.
World Wide shipping takes around 10 days to arrive, depending on it's destination.
Displayed image size of the painting may vary from the actual size.
If you have any questions about for instands a tailor made order or discount when purchasing more then one painting please let me know. I'll be happy to answer all your questions.
Dee Brown Artworks-- Copyright © All rights reserved
Materials used:
acrylic paint and structure
weathered white lines (100 x 100 cm) Dee Brown (2019)
Acrylic painting by Dee Brown
- Acrylic painting on Canvas
- One of a kind artwork
- Size: 39.37 x 39.37 x 1.57 in
- Ready to hang
- Signed certificate of authenticity
- Style: Abstract
- Subject: Abstract and non-figurative
This artwork is sold by Dee Brown from Netherlands


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wwwc21 necesitaba un nuevo diseño de página web y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 13 diseños de 9 diseñadores freelance.
Smart Discipline
You'll learn a lot by visiting our current site at http://www.thesmarttransformation.com
NOTE: there's no need to redesign the entire page. Just from the top banner down to the Instant Free Bonus box underneath the video. Once the style and overall look is achieved I'll award the price and will redesign the rest of the page myself keeping to that style.
We sell products that help parents discipline their kids
Parents with kids that are causing problems. Doesnt have to be huge problems just your every day fighting and bickering. Parents with kids who wont do what was asked of them the first time.
Just a really professional sleek looking design.
I don't want anything that looks like it came from a template. Feel free to redo the logo a little bit or use this logo: http://smartdiscipline.com/common/i…r/logo.jpg
I want to highlight the "free trial offer" and the "60 seconds flat bonus" I also want to add a box on the right that really gets peoples attention to sign up for our newsletter.


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Makeover Your Bedroom with Area {A Giveaway!}
Posted by
DesignPublic.com |
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17 Comments » | Published in Art and Artists, Bedding, Bedroom, Contests, Design Public Sales and Specials, General, Givaway, Sale | 17 Comments
If you're not already familiar with the amazing modern bedding from our friends at Area, then you should remedy that, pronto. Area is a small independent company that creates products for the modern life. They have an incredible line of furniture and bedding that is based on the modern Scandinavian concept of good design for everyday living. Area started as a collection of screen-printed cotton duvet covers and has expanded immensely since their inception in 1990, and now they make a wide variety of products such as organic cotton sheets, linen duvets, and blankets made in baby alpaca.
I was able to speak with Area's designer, Anki Spets, on the phone about a month ago and was struck by her dedication to a product that encompasses beauty, simplicity, modernity and practicality in design. Her talent for imbuing her products with both refined design sensibility and a human touch is what makes each collection truly distinctive.
Anki and I spoke about the most important place in the home – your bedroom. A bedroom should feel comfortable and peaceful, and your bed is the place where that comfort begins. I was recently researching Feng Shui for the bedroom and stumbled across an article which states "a 'good looking' and well-balanced bed is very important in creating a perfect feng shui bedroom. A good mattress, solid headboard and high quality sheets from natural fibers are also very important in creating harmonious feng shui energy."
What does your bedroom, and namely your bed, look like? Are you creating a peaceful environment for your mind and body to rest and recharge or do you have a mish-mash of bedding products that just make you look, well, confused?
Area wants to help you by offering one of our very lucky readers the chance to do a little spring cleaning in their bedroom, and give their bed a complete makeover. The winner of our giveaway will receive the following from Area – 1 fitted sheet, 1 flat sheet, 1 duvet cover, 1 blanket, 2 pillow cases, and 1 body pillow with insert. That's a value of over $1,000.
To celebrate, we're also offering 15% off of Area's new EDITH & SWEA lines which are both gorgeous and decadent. Also, don't let the giveaway stop you from purchasing something – if you win the contest and you've purchased the bedding, we will fully refund your money – so make sure you snatch it up now while it's on sale!
Ready to enter – we have lots of ways to win! See the entry form below — good luck!