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Even if Boulder County commissioners don't follow Longmont voters' lead in banning fracking, the county should at least extend its current year-long moratorium on accepting new applications for oil and gas drilling — set to expire Feb. 4 — several speakers urged during a Tuesday, Nov. 13, public hearing.
Commissioners Cindy Domenico, Will Toor and Deb Gardner are reviewing proposed revisions to county land-use regulations about oil and gas exploration outside the boundaries of Boulder County's cities and towns. After nearly a four-hour long Tuesday night meeting — including nearly 21/2 hours of public testimony — the commissioners decided to postpone further consideration of those proposed rules until Thursday morning.
Meanwhile, many of the more than 30 people who marched to the county Courthouse's hearing-room microphone on Tuesday noted last week's decision by a majority of Longmont voters to impose a ban on hydraulic fracturing, the process of injecting a mixture of sand, water and chemicals to free up underground oil and gas deposits, within Longmont's city limits.
Boulder County should provide legal support to Longmont, some of Tuesday night's speakers suggested.
The Colorado Oil and Gas Conservation Commission sued the city earlier this year, after the Longmont City Council adopted an ordinance setting additional local restrictions on oil and gas drilling. The voter-approved fracking ban adopted in last week's municipal election is expected to be challenged in court, as well.
Before Tuesday's hearing, more than 60 people gathered on the courthouse plaza on downtown Boulder's Pearl Street Mall, many of them carrying signs specifically calling for a Boulder County ban on fracking.
Stiffer county regulation of oil and gas drilling and production, one of those signs suggested, will only "regulate the amount of harm done to us."
But Jeff Robbins, a Durango attorney working for Boulder County as a special counsel as it crafts new oil and gas rules, predicted at Tuesday night's meeting that any county ban on the use of the fracking process in drilling for oil and gas would be challenged in Colorado courts and likely would be overturned.
Kate Johnson, who said she lives on a small farm on the 11000 block of North 66th Street near Longmont, was one of those calling for an extension of the county's moratorium while more studies are conducted about the potential health and environmental impacts of fracking.
The oil and gas industry wants to begin drilling in Boulder County as soon as possible, Johnson told commissioners, "but don't let their hurry become your hurry."
Audy Leggere-Hickey, a Boulder resident, said that not only is more scientific study of fracking needed, "the political landscape is evolving locally, too."
Leggere-Hickey said the nearly 60 percent of Longmont voters who supported the anti-fracking Amendment 300 came "despite heavy spending by the opponents.
"That was in Longmont. How much higher would the result be in Boulder, Lafayette or Louisville?" Leggere-Hickey asked. "I think it's pretty safe to say that Boulder County citizens do not want hydraulic fracturing and its operations."
"Longmont has spoken," said Amanda Popich, a Lafayette resident. "It's time we ban fracking" or extend the county's drilling-applications moratorium.
Robert Russ of Louisville told commissioners: "I think you're hearing a common message here: Until we know what we're dealing with, we don't want to deal with it."
While most of those speaking at Tuesday night's hearing were critics of oil and gas drilling, Jamie Jost, identifying herself as a representative of two oil and gas companies, Encana and Noble, said those companies want to ensure the county's proposed recommendations are workable.
She noted that oil and gas companies already are subject to federal and state regulations and that Boulder County should avoid duplicating those requirements. She suggested, however, that the companies are willing to continue working with the county on its effort to develop local rules that would protect human health and the environment but that wouldn't conflict or duplicate the state and federal regulations and laws.


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U.S. Army War College >> Strategic Studies Institute >> Publications >> Details
Population Diversity and the U.S. Army
Edited by Colonel Lloyd J. Matthews USA Ret., Dr. Tinaz Pavri.
Other Studies by Keyword
+[diversity] +[recruiting] +[demogrphics] +[minorities] +[women] +[ethnicity] +[hispanics] +[russia] +[racism] +[prejudices] +[sexism] +[Matthews] +[Pavri]
Brief Synopsis
The Army War College, through its Strategic Outreach Program, makes a concerted effort to connect with various American civilian entities not only to promote mutual understanding, but also to find collaborative solutions for military problems that ultimately affect all Americans. Spelman College, positioned at the core of the network of historically black colleges in Atlanta and having a student body of black women, provided an ideal venue for examining issues regarding demographic and ethnic change that we expect in America over the next several decades. We should acknowledge as well the yeoman assistance of the U.S. Army Recruiting Command located at Fort Knox, Kentucky, which provided to conferees useful briefings wholly devoid of academic vapors, focusing rather upon the unvarnished realities of future projections of the composition of America's population. Our Recruiting Command colleagues also discussed in clear terms the Army's efforts to enlist women and members of other diverse groups in the recruiting market of today-- and what we can expect tomorrow. This book is not intended as a transcript of all conference proceedings. Rather, it is an anthology of selected presentations that not only portrays the main challenges confronting those who must staff the future force in the face of unprecedented demographic flux, but also provides the attitudes and hopes of women and minorities who are part of today's Army. The reader should pay particular attention to Chapter 2, which examines the Russian military. Its author, Robert Pringle, gives us a case study in what happens when an institution collapses because it can not accommodate diversity and change.
You may also be interested in the following titles:
Command Decision: Ethical Leadership in the Information Environment
Creating Great Expectations: Strategic Communications and American Airpower
Still Soldiers and Scholars? An Analysis of Army Officer Testing
Also by the /Editors:
Winning the War by Winning the Peace: Strategy for Conflict and Post-Conflict in the 21st Century
The Future of the American Military Presence in Europe
Challenging the United States Symmetrically and Asymmetrically: Can America be Defeated?
The Political-Military Rivalry for Operational Control in U.S. Military Actions: A Soldier's Perspective
View other pubs in the following categories:
Download it Now!
- Download Format: PDF
- PDF File Size: 0.30MB
- Study is: Available via Download Only
- For out of stock or digital only publications, refer to the new GPO on-demand site. For a small fee, receive many prior publications. Click here to visit.
- All materials on our website are available as a free download.

subset: commoncrawl


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With unemployment locally at a nearly 20-year high, workers at Springfield-area food pantries say they are seeing a lot of first-time visitors walk through their doors.
With unemployment locally at a nearly 20-year high, workers at Springfield-area food pantries say they are seeing a lot of first-time visitors walk through their doors.
"The number of people seeking help has been gradually going up since last fall," said Janet Nelson, supervisor of the Catholic Charities' Holy Family Food Pantry.
"Especially in the last four to five months, we've been noticing a lot more new individuals or families who have never been through our program. We think it's related to the economy — food prices, gas and things like that."
The Catholic Charities' pantry served about 1,066 families during the first four months of 2007. During the same period this year, the number ballooned to 1,198 families, an increase of 132.
On Monday, two of the people at the pantry were Bill Williams and his wife, Holly. Holly has a job, but Bill said he has been out of work for about six months.
"This will get us by for about (a) week," Bill said as his food was packed up. "We had a lot of bills to take care of last week, so we kind of cut it short on food. It's either the food or a place to live. That's why we came here, so we could get some food and have both."
Bill worked at an auto body shop until it closed. He said he hasn't had any luck finding a new job, and the couple is supporting three children, ages 13, 9 and 4.
"It's just hard trying to survive paycheck to paycheck. As soon as I get work, hopefully we won't have to come to a place like this," Bill said.
Not that he doesn't appreciate the food from Catholic Charities.
"I'm glad they're here to help," Bill said.
Daryl Smith was another first-time customer at the pantry Monday. He said he does odd jobs that don't always earn enough money, so he needed help getting food for himself and his four children.
"This will help out a lot," Smith said.
Many of the new clients have jobs, Nelson said. Sometimes, two in a family are getting paychecks.
But it's not always enough.
Some people, especially those earning the minimum wage, are finding it difficult to cover all of their expenses in the face of a rising cost of living.
"Some of them just need that one-time assistance to help them through that difficult time. But then again, we are seeing some who are on fixed incomes, the elderly and physically or mentally handicapped people who are coming in more often," Nelson said.
At Kumler Outreach Ministries, executive director Michele Tucker said she's seeing the same thing.
"We have been seeing some new people that we haven't ever served in the past. Also, a few who would only use us during a major crisis are kind of having to depend on us a bit more," Tucker said.
Like Nelson, Tucker said she believes the main reason is rising food and fuel costs.
"People are making choices between food or medicine, and now you have the gas issue," she said.
John Reynolds can be reached at (217) 788-1524 or firstname.lastname@example.com.


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Sparks fly in the Hamptons when "regular guy" Wade Walker (Craig Robinson) crashes the preppy Peeples family reunion to ask for their precious daughter Grace's (Kerry Washington) hand in marriage. Wade might be a fish out of water among this picture-perfect East Coast clan always trying to keep up appearances, but he's not about to let himself sink. Instead, in a wild weekend of fun, dysfunction and hilarious surprises, Wade is about to discover there's room for all kinds of Peeples in this family, no matter their differences. Writer and first-time director Tina Gordon Chism (writer of DRUMLINE) joins forces with Tyler Perry to present a laugh-out-loud look at the family ties that freak us out ... but bind us together with love.
"Peeples" stars Craig Robinson, Kerry Washington, David Alan Grier, S. Epatha Merkerson, Tyler James Williams, Melvin Van Peebles, and Diahann Carroll, and is written and directed by Tina Gordon Chism. "Peeples" is a presentation of Lionsgate and 34th Street Films, and a 34th Street Films/Paul Hall/Stephanie Allain/Lionsgate production.


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This is Not a Conspiracy Theory
For international orders, please add an extra $10 for shipping.
I just completed a project called Everything is a Remix. If you haven't seen it, please do check it out because I think it's more persuasive than anything I can possibly say here.
I'd now like to introduce you to my next project, which, with your help, I'd like to launch this election year of 2012.
It's called This is Not a Conspiracy Theory and it's a multi-part series that will explain the major ideas, events and human quirks that have shaped where we are right now politically. It will center on the United States, but the stories will cover the world and I think it'll have strong relevance regardless of where you live. It will be a series with huge scope, but it'll be rooted in solid storytelling. I'll use history, science, psychology and economics to tell this story and I'll make it relevant, accessible and seriously entertaining.
It will primarily be a work of perspective. It's intended to give you some clarity and some context about where we are now. But it will also be my view of the material. It will have bias. I think it needs some commentary because frankly some ideas are bad, and this needs to be said. I'll demonstrate these things and I'll do it without cheap-shots or demonizing anybody.
I'll also be raising my filmmaking to the highest level I can take it. And I'll do that by hiring the best collaborators I can get. So I'm going to raise my own game, I'm also going to add some all-stars to the team.
The first episode will land before the election. The rest, and I'm not how many there will be yet, will come afterwards.
This will be a truly free piece of media. It will be distributed under a Creative Commons Attribution license, meaning you will be able to use this media for any purpose so long as you provide attribution. You'll be able to copy, redistribute, broadcast and remix to your heart's content.
It will also be open. I will be sharing everything I know and learn throughout production. During the initial development that access will be limited to my KickStarter backers, so you can get involved or you can just see what goes into the sausage.
So that is a quick overview of This is Not a Conspiracy Theory. I hope you'll consider backing this project. I think the series will serve as a productive discussion piece, a good introduction to 21st century politics, and a really compelling piece of entertainment. I think it'll be a positive cultural contribution at a time when we could use it. Thanks very much for your consideration.
If you have any questions, please don't hesitate to get in touch.
Again, for international orders, please add an extra $10 for shipping.
Funding period
- (30 days)


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Football Shirts, Shorts & Socks
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Home » Products » Club Shop » Tonbridge Angels FC » SNR – Tonbridge Angels FC Polo shirt
£24.99 inc VAT
The NEW Macron Tonbridge Angels Fc Polo shirt come with the embroidered club logo to the front. The Zouk Polo shirt comes in a slim fit and is short sleeve. This is made from 100% Cotton.
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or (01424) 852225
In this section you will see products and garment related to Tonbridg Angels Fc. Due to the products being custom made it can take up to 10 working days to dispatch your order so please keep this in mind when ordering for specific deadline i.e birthdays or sporting events.


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More on the Samsung Galaxy S3
Given that the Samsung Galaxy S2 is currently the world's most popular Android phone, anticipation for its imminent successor is understandably running very high. Although you could argue that the dual-core Samsung Galaxy S2 is pretty fast, any high-end smartphone launched in 2012 will need to have a quad-core in its spec list to keep tech-heads happy.
And so it is with the Galaxy S3, which is due to be the first handset using Samsung's own quad-core Exynos processor. According to a report in the Korea Times, the S3 will employ an Exynos 4412 chip running at 1.5GHz. This is claimed to be 26 percent faster than current processors and, possibly, 50 percent more efficient on battery life too.
It looks like it will need that extra speed, if rumours of a large 4.65in HD screen with 720p (or even 1080p) are to be believed. So if it can actually drive that kind of display while draining less juice from the battery, it will be welcome news.
The processor incorporates four ARM Cortex-A9 cores but the 32-nanometer chipset is a component expected to be made by Samsung itself, instead of an third party supplier such as the US-based Qualcomm.
It will go head-to-head with other new Android quad-core handsets such as the HTC One X, LG Optimus 4X HD and Huawei Ascend D Quad.
Via The Korea Times


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India, Nepal, Bhutan, Bangladesh & Sri Lanka
Click images below to enlarge:
India, Nepal, Bhutan, Bangladesh & Sri Lanka
This product is no longer available :(
Product Overview
- Product No:
- 8669
- Publisher:
- Marco Polo
- 9783829739184
- Edition:
- 2nd Edition
- Edition Date:
- May 2009
- Size:
- 130 x 250 x 12 mm
- Weight:
- 220 g
- Format:
- Folded Map
- Scale:
- 1:2 500 000
- Sales Rank:
- 26254 (See Top 100 in Maps)
Product Description
This Marco Polo map of India, Nepal, Bhutan, Bangladesh and Sri Lanka features really easy to read cartography. Topography is highlighted with relief shading and loads of spot heights and the road network is clearly shown with roads ranging from motorways all the way through to minor secondary roads. The map also clearly indicates airports, places of interest, scenic/tourist routes, view points etc.
The map includes a booklet attached to the cover that contains some useful information and a very extensive index that covers locations, places of interest and geographical features. Also included are a distance/driving time chart, a fold out flap on the cover with an overview map of the region covered and a reasonably detailed enlargement of Reykjavik.
A unique feature of the Marco Polo maps is "mark it" stickers, a set of seven reusable stickers (ie they don't damage the map when removed and placed elsewhere) that can be used to highlight interesting places, the route you plan to take (to make navigation easier), or whatever you want really.
The legend is in a multitude of languages including English.
Current reviews for 'India, Nepal, Bhutan, Bangladesh & Sri Lanka'
No reviews yet, but you should totally write one. It's easy, just put some words and stuff in those boxes over there and everyone will be super happy.


text:
I got serveral questions about Graphics in Java.
- Is it possible to detect Drawn Images/Rectangles without knowing the x & y positions
- Is it possible remove recangles? I know it's possible via "g.clearRect" but then at the place where the rectangle was is a black recangle now probaly because i work with a transparent background with a wood pattern.
- Same question about but then with images no clearImage function found.
- So above is the option about removing but isn't it possible to replace an drawn image with another one? I tried to draw another image on a certain x & y location but it just draws over it.
- Another option (maybe, just brainstorming atm) it is possible of keeping track of positons where you draw something what if you make a seperate array with the places where the second image must come is it then possible to remove everything that Graphics has drawn (in a clean way so there are not black holes). And then just redraw everything from those arrays?
Kind regards.
PS: Sorry for bad english, was in a terrible rush when I typed this.


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Replacement Lens Sets for 2018 or newer TR-90 Nylon material frames - please select the style of your lens with drop down option.
IMPORTANT NOTE: Please contact us about our special offer for existing customers that would like to upgrade to newer frames.
Lens Kit For Use With The Following Frame:
Safety Rated Lenses and Frames
ICICLES EYEWEAR is committed to providing excellence in technology and construction for each product we design. Our products meets the American National Standards Institute (ANSI) requirements, and is marked with the ANSI Z87.
We are passionate about our product that we provide to the marketplace. In recent studies, according to the American National Standards Institute (ANSI), workplace eye injuries are a leading cause of eye trauma, vision loss, and blindness. An estimated 2,000 eye injuries occur in the workplace every day. The American Academy of Ophthalmology states that nearly 90 percent of all eye injuries could have been prevented by using the right kind of protective eyewear.
The following product sizes are recommend for Large, Medium and Small Face Shapes. If you not sure, our sales representatives can make a recommendation.
Frame Size:
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Big Daddy Bagger
Cruiser Large
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Cylinder Aluminum (Small Lens)


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The Most In-Demand Jobs for Bankers
Bank bonuses have been paid out and more than half the employees surveyed by recruiter Ambition Group are looking change jobs. Ambition's Mark Enticott talks about which banking jobs are in high demand.
This transcript has been automatically generated and may not be 100% accurate.
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text:
What we all feared is true: F1 test driver Maria de Villota passed away in her hotel room due to complications from her horrific crash in July of last year.
The information comes from a statement released by de Villota's family, as reported on Sky Sports F1.
Maria left us while she was sleeping, approximately at 6am, as a consequence of the neurological injuries that she suffered in July of 2012, according to what the forensic doctor has told us.
It's terrible to hear of a driver of an F1 car being killed from a horrifying crash at a circuit (though the crash happened on the way to the pits at a straight-line test), but the family statement continues in a more uplifting tone.
Maria is gone, but she has left us a very clear message of joy and hope, which is helping the family move on in these moments.
Photo Credit: AP


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Fire destroyed a home on Cedar Pond Wednesday night.
Just a portion of the house at 18 Cedar Lake Drive remained standing around 8:15 p.m. as firefighters rolled up their hoses and left the scene.
Around two hours earlier, firefighters had arrived to find the building engulfed in flames, Sturbridge Fire Lt. John Marinelli said.
There were no injuries, and there was nobody home when the fire started. Three alarms were struck to bring in more manpower, Lt. Marinelli said. He said he was not sure how many people were displaced.
Firefighters used a hydrant at the nearby Burgess Elementary School for water.
The state fire marshal's office was called to investigate.
Firefighters from Southbridge, Spencer, Brookfield, Holland and Brimfield assisted Sturbridge firefighters. Firefighters from Warren covered the station, Lt. Marinelli said.


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Moderators: Three Stars, dagny, pfim, netwolf
columbia wrote:I got an email from Kernegie Melun and this year's Spring Carnival concert...
Friday 4/19
That's free, btw.
Gaucho wrote:Waxahatchee anyone? Should be right up Troy's alley. I'm undecided yet.
columbia wrote:You wanted to hear Linda McCartney produced by Lee Scratch Perry?
Morrissey has canceled all remaining dates on his planned North American tour.
Citing "medical reasons" -- the singer has been suffering from a series of mounting health issues over the past months, including a bleeding ulcer, Barrett's esophagus and double pneumonia -- a statement says the former Smiths frontman has chosen not to proceed with 22 planned dates across the U.S.
"Despite his best efforts to try to continue touring, Morrissey has to take a hiatus and will not be able to continue on the rest of the tour," the statement reads. "Morrissey thanks all of his fans for their well wishes and thoughts."
The tour, delayed by health issues, nonetheless kicked off with a bang in Los Angeles with back-to-back dates earlier this month at Staples Center and Hollywood High School, in which the singer appeared to be in good health, spirits and voice.
Disappointed fans are directed to collect their refunds at point of purchase.
viva la ben wrote:Morrissey is a real scumbag.
FreeCandy44 wrote:I've been meaning to pick up both the PJ20 book and Blu Ray. It is very hard to sing any Chris Cornell parts but that dude did a good job. BOH actually ain't to bad.
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text:
Have you taken the kids bowling at 1st Bowl in Town Centre Bury? Please leave a quick comment below to let us know what you thought.
Founder & Director at ReallyKidFriendly
Janis is a cheery and versatile digital expert with a healthcare background, usually seen either geeking out or sprinting through North London trying to catch her kids, Mads and Danger Boy. Thanks to her two boisterous rascals, she has become an expert in soft play areas, parks, energetic music classes, and where to get a stiff drink once they've gone to bed.
Latest posts by Janis (see all)
- Advice for the first day of school: School uniform, school shoes and name tags - August 26, 2018
- Thor: Ragnarok review – Funniest Superhero Movie Ever - October 19, 2017
- Summer Reads: Prize giveaway PLUS a monstrously funny Darkmouth interview - July 8, 2017


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Randell necesitaba un nuevo diseño de logotipo y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 283 diseños de 58 diseñadores freelance.
Devonport Baptist Church (Life Way)
Church family following God's way to life with purpose, passion and prayer
Devonport Baptist Church in Tasmania, Australia has branded our building complex Life Way. We would like a logo to include on the signage and marketing of the facility.
The design needs to work with our identity statement "We are a family following God's way to life with purpose, prayer and passion"
The design wants to be fresh and relevant and in some ways new, while maintaining some foundational element, if that's all possible!
The design will need to be fairly simple and legible for use on exterior signage including a freestanding sign adjacent to the local road.
The design could possibly incorporate a pathway to identify the journey from where we are, to where God takes us as we Discover Jesus, Become like Jesus and Communicate Jesus' Love.
Must contain the text "Life Way" and the by line "Following God's way to life with purpose, prayer and passion" or a shortened version with "purpose passion prayer" and either Life Way or LifeWay.
The project may evolve further for inclusion in a website redesign.
Descubre cómo funciona un concurso de diseño u obtén más información sobre nuestros servicios de diseño de logo.


text: We love Halloween, and we love parties, so we DOUBLE-love Halloween parties! Here are our favorite Halloween invitations to help you get in the spirit, too. Have any fun Halloween party ideas? Leave a comment below! You may also like: The best Grandparents Day cards on the entire internet The […] Read More »


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The Azure Code Samples are currently available in English
Developing a .NET console app using Azure Cosmos DB
This sample shows you how to use the Azure Cosmos DB service to store and access data from a .NET console application.
For a complete end-to-end walkthrough of creating this application, please refer to the full tutorial on the Azure Cosmos DB documentation page.
Running this sample
- Before you can run this sample, you must have the following prerequisites:
- An active Azure Cosmos DB account - If you don't have an account, refer to the Create a database account article.
- Visual Studio 2013 (or higher).
2.Clone this repository using Git for Windows (http://www.git-scm.com/), or download the zip file.
3.From Visual Studio, open the GetStarted.sln file from the root directory.
4.In Visual Studio Build menu, select Build Solution (or Press F6).
5.Retrieve the URI and PRIMARY KEY (or SECONDARY KEY) values from the Keys blade of your Azure Cosmos DB account in the Azure portal. For more information on obtaining endpoint & keys for your Azure Cosmos DB account refer to View, copy, and regenerate access keys and passwords
If you don't have an account, see Create a database account to set one up.
6.In the App.config file, located in the src directory, find endpoint and authKey and replace the placeholder values with the values obtained for your account.
<add key="EndPointUrl" value="~your Azure Cosmos DB endpoint here~" />
<add key="AuthorizationKey" value="~your auth key here~" />
7.You can now run and debug the application locally by pressing F5 in Visual Studio.
About the code
The code included in this sample is intended to get you quickly started with a .NET console application that connects to Azure Cosmos Db.


text:
We love digital - Call
03332 207 677 and say hello - Mon - Fri, 9am - 5pm
Call 03332 207 677
Last year there was a host of blog posts and thoughts on Semantic Search including this and this on the Koozai blog. A question to Matt Cutts in 2010 asking 'What is the future of Semantic Search' showed some early insight into what Google may be working towards. Let's take a look at what we know.
When a web user searches for a particular query, the chances are that this query can be written in different ways and search engines would match it to results using plurals and synonyms of your query. This is the traditional way that search has worked and it has done a decent job of returning relevant results. Along with the recent algorithm updates that have cut down on the spam and manipulative ways to have a site show for a given search query, Semantic Search will make a bigger difference to answering a user's search query with the most relevant results.
Traditional search algorithms match search queries with content and order the results by relevance and authority, loosely speaking. What semantic search is going to do is show you very detailed, relevant results through understanding your query in more depth.
Humans are very good at interpreting the use of language to associate words and topics with those mentioned or not mentioned. Computers are not great at this, but they are learning.
Google is always getting smarter and services such as Wolfram Alpha has been designed to only return informational results based on a search query.
You may have noticed the visual changes in the Google search results in recent months. Knowledge graph information, weather, sports scores, answers to maths questions, image result suggestions, keyword phrase suggestions. These are all part of the growth of Semantic Search and designed to help return a more relevant result to a query.
When you search for a brand such as Google in Google (yes the internet won't explode if you do this) then you will get a nice company summary on the right hand side. Providing this information what is referred to as Knowledge Graph and is part of Semantic Search.
Search for 'What is the weather in London this Monday' and you'll see the information right there. Google understands the key elements in your use of language.
Say I wanted to know the latest football score of my local team, a quick search using 'Southampton FC latest score' returns the following:
Inputting a calculation into Google such as 'what is 25 times 4 divided by 2' will bring up the calculator function with your calculation. This is just what I wanted to see, not a list of snippets of calculations from various websites.
When searching in Google Images, you are typically shown several sub options if your search is broad or has double meaning. For instance, if I search for 'picture of a keyboard', Google will show me the most popular type of keyword which is a typical computer keyboard. Along the top there are shortcuts to re-phrase your query with the next most popular ones: 'picture of a music keyboard', 'picture of a piano keyboard', 'picture of a laptop keyboard', etc.
It's increasingly common to see Google suggest a completely different way of phrasing a search query. Google is almost trying to get users to 'speak clearly' in order to build up its understanding of what are the most common ways to ask things.
As you can see in the previous examples, some queries are understandable but some want you to refine your query to some more relevant results. Google has been unable to understand the context of your search and needs further help.
In the future we should see Google understand more of what you were thinking via your website browsing behaviour (when logged into your Google Account) and how you interact on social networks such as Google+.
Computers need to gain more of an understanding about who we are; much like our friends and family know us, Google will become smarter at learning our interests and personality.
Typically mobile users search for shorter keyword phrases and demand quicker, concise answers. What can Google do to help break down a user's query and return a highly relevant result? Well location will play a large part in what is returned. The obvious one is if someone searches just the words 'Italian restaurant' Google is going to understand that you are probably looking for a local Italian restaurant to eat at now or in the near future. Other factors could include what device or mobile browser software is being used.
One way mobile search is looking to overcome the shorter search queries is through the use of voice. Google has voice search on android devices and iOS has Siri and dictation software built into its devices. This brings out longer more natural queries from users, however the search results need to interpret these well and return the right results. This is where Semantic Search will excel.
It's not just Google that would help its users with Semantic Search results, ecommerce sites and Travel sites, for example, can help improve their conversion rates if they can better understand their visitor's queries. Users may search for 'somewhere hot at Christmas' or 'child friendly hotel in school summer holidays'. Traditionally these queries would struggle to return relevant results to a user. If an algorithm is smart enough to understand the Semantics then they would know that the user wants a holiday in the southern hemisphere for a number of days either side of December 25th, or the second user might want a hotel with a shallow swimming pool with availability anytime between the third week in July and the second week in September.
One example of a site getting ready to roll out Semantic Search on a huge scale is Facebook with its 'Graph Search'. This will allow users to search for answers using a natural language such as 'Which of my friends have been to Italy' or simply 'People who like Cycling'.
Webmasters can't game the semantic search results as much as they might have been able to fool the search engines in the past with poor quality link building and keyword spam. As they become smarter at understanding what people are asking, more specific search results are going to be delivered.
Online marketers can capture traffic and business from the future Semantic Search results by considering these tips:
If you've got some good examples of existing implementations or have predictions on what else we might see changing, leave a comment below.
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Word Cloud – Web 3.0 Via BigStock
Unlike 08 numbers, 03 numbers cost the same to call as geographic landline numbers (starting 01 and 02), even from a mobile phone. They are also normally included in your inclusive call minutes. Please note we may record some calls.


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Against one of the walls here in the Squarespace office, there's a huge black shelving system brimming with books and magazines. Much like a good rug, it really ties the room together—however, it's also a functional library with an eclectic selection ranging from programming tomes to elaborate design anthologies. That's a combination perfectly suited to describe the engineer/designer mix that makes up Squarespace.
Flipping through some of these publications, it's easy to see how simple content like text and images can be presented in a myriad of different ways. The programming books are laid out in a linear stream, the layout of each page identical to the one that came before it. It's pragmatic and useful, which is also a great way to describe how content management systems have dealt with content in the past. Pick up one of the fashion magazines, art books, or typography manuals, and it's a completely different world: text and images are juxtaposed, split into columns and laid out in collages. It's a very inviting presentation, both complementing and enhancing the content.
We looked at this and asked ourselves: "Why can't we do this with Squarespace? Why hasn't the web caught up?"
Well, it's complicated.
Printed content has a single reality; what you see on a page is the same to everyone. Content on the web—even ignoring underlying technological issues—is not. There's no telling which device or browser a page will be viewed with, at what resolution or with what user settings. The same page has to work on everything from a desktop computer to a phone, and preferably adapt to those conditions while maintaining the intended experience. The very flexibility which makes the web so wonderful also makes for a very complex design environment; the necessary skill to make even very simple layouts becomes a staggeringly tall order for the majority of users, even if they do figure out how to wrangle the underlying HTML and CSS.
We built LayoutEngine in an attempt to narrow this gap between print and web: to make building great layouts as easy as simply moving your content where you want it, while keeping the underlying structure and code equally elegant and simple.
It took many iterations and several breakthroughs, both in terms of figuring out how to build an interface for something like this—early versions were radically different—as well as how to deal with the underlying code, which was paramount for the resulting output to be flexible and compatible across the full web landscape of browsers and devices.
As we iterate further, LayoutEngine will become more and more sophisticated and powerful. But we'll talk more about that at a later date. This first version is a major step towards our goal; we're exceptionally proud of it and we can't wait to see what you make with it.


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Installing Your Fire Bottle The Right Way
As a driver, there's no greater fear that I have than trying to get out of the car while it's engulfed in flames. Every racer has seen those videos of drivers who panic while trying to get out of the car and then end up with second- or third-degree burns. I always find myself saying while watching those videos, Why aren't you pulling the fire extinguisher?
I would venture to say that most drivers don't give a second thought to how well their fire bottle is installed. Most people have the mindset that as long as it will pass through tech then it's fine with me. The problem with this type of thinking is when the worst happens and you go to rely on your fire extinguisher, it may or may not work. Take a few hours and make certain that your fire bottle is installed properly so you don't have to worry about whether or not it will extinguish a fire.
Choosing the right fire bottle is the first decision you have to make. To the naked eye, any fire bottle will do, but you need to choose the right one for your type of application. The system that is being installed in our Circle Track project car has a 5-pound bottle designed for two ports which we decided would be sufficient for our application. The two-port setup will allow us to have one port in the fuel cell area and one by the driver's seat.
If, however, we were running an 850hp, alcohol-burning, Outlaw-style Late Model, I'd feel more comfortable with three extinguisher ports. The third one would be placed in front of the firewall near the fuel pump to give maximum protection from the invisible fires associated with alcohol-based fuels.
If we had installed a three-port system, we would need a 10-pound bottle since the 5-pound one won't supply enough pressure to fire all three ports.
The placement of the fire bottle is crucial as well–I wanted to put it as low as we could and as far left as possible. Fire bottles can weigh up to 10 pounds and that's weight you can use to help with your setup.
The installation is key to making certain that the bottle will work properly when the firing mechanism is pulled. Each fire bottle is made up of the bottle itself, a gauge, and the firing mechanism. We've already talked about the bottle, so let's discuss the gauge. The gauge tells you whether or not your fire extinguisher is properly charged. If the gauge is reading in the red at all, you need to have the fire bottle recharged. Don't take any chances.
The firing mechanism is made up of two safety pins and the firing pin. When that pin is pulled or activated, it will shove a sharp piece of steel through a thin sheet of metal allowing the fire extinguisher to release its chemicals.
Most fire extinguishers work to smother the fire by removing the oxygen out of the immediate area. This is why you don't want to point the extinguisher port at the driver's upper body. You won't be able to breathe when it goes off.
To avoid this situation, we placed the port in the driver's compartment where it will shoot my lower torso. This will protect my upper torso as well, but it will do so while still allowing me to escape the vehicle. Now follow along as we inspect and install our fire bottle the proper way.
Now we have to install the firing mechanism in a location that will be easily accessible to the driver. The easiest place to do so on most vehicles is the dash. But the leg braces on the seat will work as well. To do this, we unscrewed the firing mechanism and drilled a 3/8 inch hole into the dash.
This is a perfect location for the firing pin. Notice how we have taken a zip tie and wrapped it in duct tape. This allows the driver to pull the safety pin and the firing mechanism easily.
Now we need to install the rear and front extinguisher port. We'll use a splitter that will push the chemical to the driver's seat and the rear of the car. Here's everything that we will need. You might notice that we have two different types of splitters. The bottom splitter and fitting is a compression fitting. The top fitting is a flare-style fitting. Both have advantages, the compression fitting doesn't require you to flange the 1/4-inch pipe. As you tighten the nut on the pipe, the compression ring will seal to the extinguisher port. Whereas with the flare-style fitting, you'll have to flange the pipe, but it will seal extremely well to the port and splitter.
Next, we'll measure out how much line we'll need. Then we'll cut and flare it.
Once measured, we'll use a small pipe cutter to make a clean cut through the 1/4-inch pipe.
With the pipe cut, we need to bend it to keep it flush against the floorpan. We just follow the bender here and put the 90-degree bend in it that we need.
The bends came out perfect, and now we just need to drill through the rear sheetmetal to the fuel cell and then hook up the splitter.
We found a spot that will work perfect for the port. Once we drill through the sheetmetal, we'll measure our line again, cut and angle it, and then attach our flare fitting.
We need to put our nut and the flare in the right order so that we can flange our extinguisher line.
This tool will allow us to flare the pipe without damaging it.
Make certain that the flare tool is centered in the pipe and then tighten it up. As it tightens, it will expand the pipe.
The bend and the flare came out great. Now all we have to do is attach the pipe and then secure it to the sheetmetal.
Here's what the port looks like from the bottom of the fuel cell. It's pointed directly at the fuel cell in case the worst happens.
Finally, we'll take several small securing brackets and rivet them to the sheetmetal every 5 inches or so. This will ensure that the line doesn't break or move during an accident.


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– EH-Net Live! October features Chris Hadnagy, Stephanie Paul and Dov Baron presenting Hacking Humans on Tuesday Oct 29 at 1:00 US Eastern Reg Open NOW!
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11 Feb Jay Mohr and David Wild
[download_link link="http://www.podtrac.com/pts/redirect.mp3/traffic.libsyn.com/theadamcarollashow/2013.02.12ACS.mp3″ target="blank"]DOWNLOAD HERE[/download_link]
Show Summary
Adam opens the show ranting about the boy at the YMCA who was mistaken for a girl because of his hair. David Wild is also in studio, and Adam talks to him about the Grammys from a decade ago, when Tracy Chapman won Best Rock Song for 'Give Me One Reason.' Wild then talks about this past weekend's Grammy's when he worked with Prince, Johnny Depp, and John Mayer (among others).
After the break, Jay Mohr joins the gang, fired up about all the Grammy talk. The guys then talk about the Stones and Aerosmith, and Adam makes fun of stars who wear scarves. The news then begins with more comments on the search for Christopher Dormer. Next up, the guys chat about Pope Benedict's resignation, and the show wraps up with a conversation about the Super Bowl Blackout and banging on a bullet with a hammer.
Subscribe to Mohr Stories on iTunes, and listen to Jay Mohr Sports, Monday-Friday 12-3pmEST on Fox Sports Radio.
Also check out http://JayMohr.com, and follow him on Twitter @JayMohr37.
You can also follow David on Twitter @WildAboutMusic.
Villaraigosa and Dorner
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Superfan Sidebar
Click Here for Superfan Giovanni's recap and commentary on today's episode.
For more, go to AdamCarolla.com/Gio.
Show Credits
Producers: Mike August, Mike Lynch, and Mike Dawson
Co-Producers: Gary Smith, Chris Laxamana, Matt Fondiler and Jeff Fox
Newsgirl: Alison Rosen
Sound Effects: Bryan Bishop


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- Product Information
The Lion King by Camelot Design Studio for Camelot Cottons
Panel size is 17" x 42"Additional Information
Product Type Panels Fabric Collection Disney Panel Program, The Lion King Fabric Designer Camelot Design Studio Fabric Manufacturer Camelot Fabrics Theme Baby and Child - Boy, Baby and Child - Girl, Licensed, Movies & Entertainment Fabric Type 100% Cotton Fabric Width 18" x 42" Image Scale 17" x 42"
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Main Category Description
Steps by steps instruction on how to use CamViewer.mobi app
audio for Toshiba wb11a...
Use this control to limit the display of threads to those newer than the specified time frame.
Allows you to choose the data by which the thread list will be sorted.
Order threads in...
Note: when sorting by date, 'descending order' will show the newest results first.


text: It took astronauts 33 days to grow enough red romaine lettuce to make a small salad. NASA hide caption toggle caption NASA The Salt This Salad Is Outta This World: Astronauts Eat Greens Grown In Space August 10, 2015 Farming plants in space may prove critical for future deep space travel. On Monday NASA announced it had mastered romaine lettuce. "That's awesome, tastes good," astronaut Kjell Lindgren declared. This Salad Is Outta This World: Astronauts Eat Greens Grown In Space Listen · 2:44 2:44 Toggle more options Download Embed Embed <iframe src="https://www.npr.org/player/embed/431343624/431357722" width="100%" height="290" frameborder="0" scrolling="no" title="NPR embedded audio player"> Transcript


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1221 Elmwood Park Blvd., Suite 411,
New Orleans, LA 70123
Property Description
Bordered by the lake and river, Kenner offers an array of experiences. From boat launches to casino betting, there's fun for all ages. In Rivertown, experience the Kenner Planetarium for an "out of this world" adventure. Kenner is also Jefferson Parish's largest city.


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Revision as of 15:00, 15 October 2006 by Dwarf Lord (Talk | contribs)
The Beleriand river Taeglin was a tributary of Sirion.
It rose in the Ered Wethrin and flowed southeast through Brethil to join Sirion on the borders of Doriath.
An important for on the river was the Crossings of Teiglin, near the western border of Brethil. A road ran through it from Nargothrond to Minas Tirith.
Minor tributaries were Celebros and Malduin.
Other versions of the legendarium
In earlier writings, the river was called Teiglin. As revealed in the The History of Middle-earth series, the rivername should actually have been spelt Taeglin. This was a relatively late change which was not adopted into the published Silmarillion, which uses the old name.


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The Pac-10 can finally consider its dance card filled.
According to ESPN, the University of Utah is set to become the twelfth member of the Pac-10, as the conference quickly moved to invite the Utes after having its expansion efforts temporarily derailed by Texas and four other members of the Big 12 earlier this week. An announcement could come as early as Thursday afternoon after Utah's board of trustees convenes to discuss their options. Officials from the Pac-10 are scheduled to appear at a press conference following the meeting.
Utah, a member of the Mountain West Conference since its founding in 1999, became a power in both football and basketball, making it a good fit for the "Conference of Champions."
The Utes move west caps off one of the wildest offseason weeks in recent college football memory. Four teams – Nebraska, Colorado, Boise State, and now Utah - have shifted conferences in the last seven days and it appears that more moves could be forthcoming.
The addition of Utah gives the Pac-10 the ability to split into a divisional format and host a football championship game, a venture that has been financially lucrative for other leagues such as the SEC and ACC. It also gives Colorado, which joined the conference a week ago, a geographic rival.
Gaining access to the Pac-10's automatic Bowl Championship Series bid was undoubtedly a major factor in Utah's decision to bolt the MWC. In 2008, Utah capped off an undefeated regular season by thrashing traditional power Alabama in the Sugar Bowl after being denied a chance to play for the national title. The Utes were also undefeated in 2004 but were not invited to compete in the championship game. The Mountain West Conference is not a BCS member and is not privy to an automatic bid.
It is unclear at this point when Utah would start athletic participation in the Pac-10 should it accept the invitation, but it could be as soon as the 2011 season.


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Door Stopper Monkey
Brighten your childs room with this super cute animal foam door stopper. Not only will it proetect little fingers from getting slammed into the door - the cute design will look so cute in their nursery & room.
- Made from foam
- Prevents door injuries when your little one discovers how to slam the door
- Cute deisgn
Share this


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The UAE Arabian Derby, a 2200m Prestige race for four-year-olds, was the highlight at Abu Dhabi on Sunday evening and was won impressively by Tenor Des Vialettes.
Richard Hills was in the saddle for his main employer, His Highness Sheikh Hamdan bin Rashid Al Maktoum on the horse having only his third start and recording a second successive victory.
Trained by Musabah Al Muhairi, he clearly pleased Hills who said: "He has only had those two previous starts and is still learning; when I asked him to go for home he was rather green and took a while to respond so hopefully he can get better with experience as he strengthens up."
Racing concluded with a 1600m maiden, the only Thoroughbred contest on the card and trainer Al Muhairi was able to complete a double with stable jockey Wayne Smith in the saddle on Penthouse Reader who was losing his maiden tag at the seventh attempt.
Never far off the pace, Smith's mount was ridden to lead close home and the jockey said: "The horses have been in good form and we hoped this horse would cope with the switch to turf. He has improved with his racing and can hopefully pick up a small handicap."
The 1600m Abu Dhabi Fillies' Classic provided the Gill Duffield-trained Al Alriaah the perfect opportunity to lose her maiden tag at the third attempt and she stormed clear under Tadhg O'Shea.
Runner-up on both her previous starts, she was able to gain revenge on both the fillies who had beaten her previously to the delight of O'Shea who said: "She is certainly improving judged on that effort and Gill has her horses in great form. It was a decent little contest and a good performance."
The opening 1000m maiden was won in style by Swyft making his debut for trainer Salem Al Ketbi under Chris Taylor.
It was a very impressive debut from this full-brother to 2009 Group One Dubai Kahayla Classic winner Fryvolous and Taylor said: "He took a while to get going but we always thought he was a horse with plenty of ability. He should be able to build on this."
Motsho Belle was a surprise winner of the 2200m conditions race in which he made virtually all under Rory Cleary for trainer Bakhit Al Ketbi.
The 2200m handicap was won by Baher who made all under apprentice Wencelas Walter for trainer Eric Lemartinel who said: "He is not the easiest horse and he has given him a great ride."


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Lyrics:First verseI was strolling to day through a garden, A garden where red roses grow, And sweet mem'ries were rising before me, Sweet mem'ries of long, long ago. 'Twas there I first met you and gave you my heart, 'Twas there that you kissed me goodbye, All that I have left now to console me, Are rose mem'ries that never will die. ChorusRoses of beautiful memories, Rose mem'ries that never will fade, Mem'ries that always will live in my heart, Though you and I have drifted apart; Come back to me, let us enter again, that wonderful garden so fair, For I've planted my roses of memories, And you'll find them all blooming there.Second verseOh, how well I remember the old days The days when I stroll'd there with you, My heart then was free from all sorrow, For you told me you'd always be true. I go there each day to that garden so fair, For I dream I might see you once more, All the roses bring back pleasant mem'ries Fond mem'ries, rose mem'ries of yore.(Chorus)
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Monday, 12 July 2010
Maybe there is more than a pot in that belly
But I know you're all scrolling down anyway, just to see whether or not I'm pregnant. When I was 5 days late for my period today, I decided it was time to buy the brand I have found to be most clearly negative in the past: "Apotekets Graviditetstest" (and thus the best)
So, I peed on one and, after 3 minutes, there was no second line. But when I returned to it 5 minutes later, gosh darn it, there was a second pink line there. 2 hours later I took another and after 7-8 minutes, there was that same pink line. I don't know, can you even see the lines?
Well, SR and I can. And we're really excited. We had actually tried to avoid (my) getting pregnant this month due to our summer vacation, Voyageur and the Ironman, but, whoops, maybe we weren't that careful.
And, just in case you aren't convinced yet that I'm pregnant (just like I wasn't), I took a 3rd test with SR and The Lorax and the same faint pink line was there.
What does a woman with a 2 week old fetus look like after a 100 km bike ride and a 20 mile run on the hottest weekend in Danish history, you ask?
Running song of the day: Rebecca St. Claire by The Modern Skirts. Actually, it's not that great to run to, but I really look forward to singing it and playing it on the guitar when we arrive in the US (and I am reunited with a guitar!).
27 comments:
- May-Britt Hansen said...
Congratulations to the both of you :-)
- 12 July 2010 at 17:53
- Meghan said...
Congrats, little family! Wow and how exciting!
- 12 July 2010 at 17:57
- SteveQ said...
Congrats on being one of the 60% of women who can correctly read a pregnancy test (med school pays off!)
Placental lactogen or chorionic gonadotropin test?
I peed on a stick today, too - that's just trail running.
- 12 July 2010 at 18:11
- Marathon Princess said...
Congratulations! :-)
Just take it easy and enjoy the upcoming races, racing while pregnant really changedmy mindset and actually made me enjoy it a little more.
- 12 July 2010 at 18:14
- olga said...
You can still do the 50 and the Ironman, may be just puke a few too many times, but then again, may be you get lucky and get by without:)
- 12 July 2010 at 18:32
- Emily Peasey said...
Oh wow, how exciting! Congratulations and best wishes to the whole family.
- 12 July 2010 at 18:35
- Ellen said...
A big congratulations!
- 12 July 2010 at 18:55
- Angie Bishop said...
How wonderful!!!! Congrats to you and your whole family :)
Yay for babies!!
- 12 July 2010 at 19:11
- Karen said...
- 12 July 2010 at 20:05
- PiccolaPineCone said...
*GIRLY SQUEAL* OMIGOD! You're pregnant! (she knows Piccola, she knows, she just just blogged about it) BUT OMIGOD you're pregnant! *GIRLY SQUEAL*
there is NO denying the existence of that pink line. GO FETUS!
- 12 July 2010 at 20:26
- Helen said...
Now go buy yourself some non-pink and blue running shorts as a pregnancy gift :)
- 12 July 2010 at 21:01
- Mile Posts by Dorothy Beal said...
Congrats! I'm a recent follower and I'm preggo too :)
- 12 July 2010 at 21:46
- mmmonyka said...
- 13 July 2010 at 00:09
- amy said...
So exciting! I look forward to following your training throughout. Congrats!
- 13 July 2010 at 00:45
- Jrahn said...
Just as I'm leaving the puke-filled first trimester, you're entering it. Congratulations!
Just remember to please take it easy on your expectations for yourself. I worry for you....
- 13 July 2010 at 01:38
- Danni said...
Yay congrats!!!
- 13 July 2010 at 02:36
YEAH!!! So many prayers and thoughts your way! Running for 2 is fantastic.
- 13 July 2010 at 04:25
- cldevall said...
Congrats SLG!
- 13 July 2010 at 04:34
- amazon said...
Congrats! You make me think maybe I haven't been patient enough with the tests I've taken...
- 13 July 2010 at 08:18
- Fast Bastard said...
Me: why are you glowing?
Sea Legs: what, didn't you read my blog?
Ok, not really.
- 13 July 2010 at 08:21
- SteveQ said...
My comment came out meaner than it was meant - I just usually reserve the congratulations for 9 months or so. If it's a girl, are you going to name her "Helle"?
- 13 July 2010 at 17:38
- Alee said...
Congratulations! I'm a new follower and love your blog. Nice to meet you!
- 13 July 2010 at 21:36
- 14 July 2010 at 08:20
Kæmpe stort tillykke til jer begge. Jeg læser først din blog nu - og er ked af, jeg ikke får sagt tillykke før nu. Hvor er det bare super skønt.
Mange tillykke-knus
- 14 July 2010 at 14:24
- cherelli said...
Congratulations SLG!!! Hope this pregnancy is just as awesome as the last one!
- 14 July 2010 at 17:28
- Diana said...
What wonderful news! Congrats to you!
- 15 July 2010 at 06:19
- Kate said...
I'm late to the party in reading this news, but many congrats to you!
- 16 July 2010 at 05:03


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text:
Lorillard CEO: The Future of Tobacco
Murray Kessler, the CEO of Lorillard, America's third-largest tobacco company, discusses what's ahead in alternative tobacco products and managing regulatory risk.
This transcript has been automatically generated and may not be 100% accurate.
... chairman and chief executive officer of Lorillard Incorporated ... the third-largest tobacco company largest electronic cigarette manufacturer in denied it ... get the traditional smokeless tobacco which is already a very big multibillion-dollar industry is so ... um there's a long way to go for furry things to catch that but it's on that path ... then you've got this news which is a new ... entry into the U S which is ... very small and I think you know I've been quoted in the path of thing that's gonna take a very long time to gain traction in the U S Senate candidate that proved out ... my prediction was correct then ... and is all bulls and I think those are ... our one so you know what I've made my bet on a cigarette ... for two reasons ... one it gave Lorillard ceded harm reduction ... table ... and I think that's where the industry needs to go ... I think that the fighting of the cast and ... battles in court ... needs to be put to bed ... and that the public health community and the tobacco industry need to work together ... on harm reduction alternatives that appeal the smokers ... that can both benefit public health and benefit business ... lender Ally and I think you'll ... see a lot of magic happen ... I'm number one way I'd get people to to join this company and I haven't made a lot of changes of the oak ... is to get him to meet a great people here are the reason ... that we have very long term tenure people are passionate ... committed ... love this company feel like there they're part of the family and I can just get people in the door ... to see that we are responsible marketers ... good people mark illegal product an irresponsible way ... with the with the company that wins the year after year after year ... on year ... there are many interviews people go on where they see ... that owning a company that has gained market share for eleven consecutive years ... has continued to create value ... has continued to weigh in on every single matter so ... I'd I should only get to do is get amended or show them the winning team ... I do believe that a top it's my job to get out there and engage in and pick it up so ... you multiple trips to the FDA multiple trips to Washington talking about the issues ... letting people see that we are reasonable and responsible and were willing to work with them ... I'm ... so you know whether was with director day ... our director Zeller ... and ... in sharing the stage sharing all the information we have that I'm talking about the opportunities that ... to me you know there's ... there's the start of the traditional business written communication legal in all that ... and then there's the old fashioned ... aam ... look let's roll up our sleeves and do some work there and see if we can work till puzzled look ... you know any product Danamon I ... tried to calm understand it now ... I then back to industry if you know my background I used to be the CEO of us smokeless tobacco company ... I'm worried its goal and I continued that its goal so I've never been a smoker and therefore his cigarettes don't really provide a ... solution to meet so I still occasionally ... image on a typical


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Electroporation doesn't like my plasmid??? - (Aug/11/2010 )
Hi all, new user here. Looks like a very helpful message board!
I am trying to clone a plasmid using electroporation and am having some difficulties. The vector and insert are both ~2.2kb, the vector being a derivative of pUC18 minus the alpha fragment of the LacZ enzyme. The insert is for a beta-gal gene.
Here is my current protocol.
Vector is taken from a plasmid prep, insert is taken from a gel purification of my PCR product.
Vector and Insert both digested with PstI for 1hr. at 37C.
Shrimp alkaline phosphatase added to the vector, and both allowed to continue incubating for 30 minutes.
Enzymes are heat killed at 65C for 15 minutes.
A T4 ligase mixture is made from these and allowed to go overnight at 16C.
At this point, if I use Z-Compenent cells that I have made, I can transform with decent efficiency. I get around 50 blue clones per plate this way.
However, when I do electroporation, I get zero clones. I electroporate both pUC18 and my vector (pre-digest of course) and both give me lawns of clones.
I have tried gel purifying the ligation reaction but still have the same issue.
Kinda stumped on this one.
I don't know my DNA concentrations, but I do know that it works with the Z-competent cells, so I wouldn't think anything needed to be changed there.
Please correct me if I am wrong.
I understand that this is a simple ligation between a vector and insert where both DNA fragments have been cut with PstI.
The first thing to do is to check your ligase, T4 DNA ligase and its buffer can easily go off. The ligase buffer has a strong smell (DTT). If you can not smell anything from the buffer it means that the DTT has been oxidized and the dATP is probably oxidized too. Bin the buffer and get a new one. T4 DNA ligase does denature at -20C.
To check that the ligase is working, do the ligation reaction between your insert and vector. Run part of the ligased DNA mix onto a gel. You should see several high molecular weight bands.. which are your ligated DNA products. If you do not see those high molecular weight bands, the ligase is dead. Bin it and buy a new one (Buy the small vial. In my experience, a lab can rarely finish a small vial before it becomes unusable.).
If you do not see, any DNA, it means that the DNA was lost during the ethanol precipitation or the 70% ethanol wash. I find that if you centrifuge the sample after adding the 70% for 12 min, you can pellet down any DNA that got detached from the tube well when the 70% ethanol was added. I also find that doing the ethanol precipitation in a 500ul tube is very helpful. A strong light can be used to help see the DNA pellet.
Oh, and did you check to see if the cut vector and DNA fragment are actually present? Perhaps by gel or nanodrop? It is possible that the reason why there are no colonies, is due to the absence of DNA, which may have been lost during the purification step.
What was the ratio (mol) that was used for the ligation?
Thanks for the reply. I know for a fact that I have ligated plasmid because I used the exact same ligation reaction to do a chemical transformation and it worked (~50 clones per plate).
The T4 ligase is new.
Is it possible for something to happen to my ligation reaction if it sits too long? I did the chemical transformation the next day, but the remainder of the ligated plasmid sat in the fridge for a week before I tried the electroporation.
I havent been doing any ethanol/isopropanol precipitations at all. The only clean up I have done has been on the PCR product, the insert, after PCR.
I actually had a similar issue recently. We had one type of Quick Ligase kit and then received a kit from another lab. The new kit wasn't working and I was getting 0 colonies when ligating. Unfortunately we didn't have any of the old ligation kit to test if it was a ligation issue or something else.
When we read the kit's manual carefully we found the issue. The new kit's buffer has PEG, which is apparently bad if you're using electroporation.
So while for the old kit we would do ligation --> heat inactivation --> electroporation and it would work like a charm, with this kit we had to do ligation --> ice incubation --> butanol extraction --> electroporation.
The butanol extraction gets rid of the salts and PEG, so electroporation can occur without a hitch.
Lesson learned for us. Hope this helps? Good luck! I know how frustrating it can be.
Also, if when you digest are you checking if the vector has linearized properly? Does your PCR product have enough of a hanging sequence for the restriction enzymes to sit on and do their cutting?
Knights on Thu Aug 12 19:32:11 2010 said:
When we read the kit's manual carefully we found the issue. The new kit's buffer has PEG, which is apparently bad if you're using electroporation.
So while for the old kit we would do ligation --> heat inactivation --> electroporation and it would work like a charm, with this kit we had to do ligation --> ice incubation --> butanol extraction --> electroporation.
The butanol extraction gets rid of the salts and PEG, so electroporation can occur without a hitch.
I was thinking it was something in the ligation reaction, but I am unsure if any of this would really prevent electroporation.
Here is what is in the T4 ligase:
300mM Tris, 100mM MgCl2, 100mM DTT and 10mM ATP
I know salts can ruin an electroporation, but 10-20 ul of a 100mM MgCL2 solution into 200ul of cells? Seems like it wouldn't be enough, and I don't detect any arcing.
I think this is probably my next step though, clean up my ligation reaction.


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Jeanne Johnson necesitaba un nuevo diseño de logotipo y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 44 diseños de 16 diseñadores freelance.
I am selling a tech company and in the process of opening a custom motorcycle repair and design company. My eldest son is finishing his training and I want to set him up in a business that repairs motorcycles, but also offers customizations. He wants to do quality repair, but also sell tanks and fenders with custom paint over the internet to people who want to give the appearance that they are 'bad to the bone' and live life 'Full throttle 'til you see God'.
The demographic Harley Davidson quotes is typically white male 42-47 year old for the repairs, and white male 21-45 for the custom paint options, however motorcyclists come in all ages, colors, and socio-economic types.
All motorcycle businesses acknowledge the demographic with the most room for potential is the female rider so we will want to keep that in mind. The temptation at first blush is to deliver art that conveys the typical - the same skull and crossbones we see everywhere in the motorcycle industry - but we are looking for something a little different.
We named it grimcustoms because the definition of grim is appropriate for the biker mentality and my son always liked the old images of the 'grimreaper.'
I graduated from art school way back before desktop publishing and I know that this is the hardest part.
I can tell you that the 'Sailor Jerry" artistic style is a favorite as it is based on tatoo art and bikers pride themselves on their tatoos. The use of the Reaper is not required, but it was in our minds when we named it. If you do anything with a cycle look to it - your work will come under technical scrutiny unless the look is more the impression of a motorcycle, rather than a specific brand or silhoutte of a type of motorcycle. (ie chopper, streetbike, Harley vs. Ninja, etc.)
We are looking for a logo to be used on business cards, postcards, repair reminder static cling stickers, and t-shirts. The ideal design would be simple and powerful and something that prints well in 4-color but also can be pared down for a 1 or 2 color option.
The industry tends to do a lot on black backgrounds - but we are open to your ideas.
Descubre cómo funciona un concurso de diseño u obtén más información sobre nuestros servicios de diseño de logo.


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Man Tracks Down Guy Who Stole His iPhone With An App & Whoops On Him!
"SAN DIEGO - A dramatic video showing a fight over an iPhone is starting to go viral. It all started on Dec. 30, when Kenneth Schmidgall lost his iPhone. He said he kept calling it, but no one would answer. His friend Greg Torkelson wanted to help. "We just wanted the phone back," Torkelson said. "Some of it was a little bit of adrenaline… you know, it was the chase of trying to get the guy."". 10 news. s/o @lolasportstalk
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Veterans Administration (VA) has a special pension with aid and attendance benefit for veterans and surviving spouses with non-service related disabilities that require regular assistance with their Activities of Daily Living (ADL's). Eligible candidates may use the benefit to purchase in-home care services.
Veterans Assistance Programs for Seniors in Northern Virginia
Our Veteran Benefits affiliates go through a 7-Step Process to determine eligibility:
Step 1. Screen for eligibility
Step 2. Obtain original military paperwork
Step 3. Collect supporting documentation
Step 4. Review completed VA application
Step 5. Provide updates throughout the process
Step 6. Assist with any VA documentation requirements until final VA approval
Step 7. Assist with monitoring medical expenses to ensure eligibility
These funding sources through the VA could pay for the in-home care services you need. Once you are identified as a good candidate for this benefit, Executive Care of Northern Virginia can typically start providing home care services within 45-90 days
Let us know if you are a veteran or a spouse of a veteran, and we will start the process.


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Lords of Middleton is a member of the Guild of Q Butchers, which means that you, the customer, can be confident that the meat you buy from us is properly stored, displayed and handled prior to purchase.
All members of the Guild of Q Butchers are committed to maintaining and promoting the fine traditions of top class butchery and have to pass regular and rigorous inspections of their premises, working practices and hygiene standards to qualify for Q Butcher Status. So you can be sure that your Guild of Q Butcher really is one of Britain's best.
The natural environment of the Scottish countryside provides that special ingredient which gives SCOTCH PREMIER products that unique flavour and wholesomeness.
The quality of the pure clean waters from Scotland's mountain streams is legendary. Add to that the lush natural pastureland, rich in minerals and nutrients and the clean unpolluted air and you have the ideal conditions for rearing high quality livestock.
Game produce is a seasonal thing and we do our best to stock the widest range possible. However if you don't see what you are looking for contact us and we will order it specially for you.
All our Venison is supplied by Scotch Premier and comes from the Balmoral Estate. So we can't see how it can get better than that.
"Venison farmed on the Balmoral Estate, fit for the Queen"


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Chuck D of legendary hip-hop group Public Enemy, is scheduled to encourage students to reclaim democracy from corporate interests during the United We Stand Festival set for May 10 at UCLA.
He is scheduled to speak between 2 p.m. and 4 p.m. on organizing, participation and education to take back democracy on the UCLA campus, according to event officials.
The political-centric music festival is scheduled to feature Public Enemy (without Flavor Flav), Immortal Technique, Brother Ali, Cappadonna, U-God and Masta Killa of the Wu-Tang Clan as well as television personality Larry King, former U.S. Representative Dennis Kucinich and others. The festival is set to begin at 4 p.m. at Pauley Pavilion at 555 Westwood Plaza in Los Angeles.
Tickets are available for the festival at the UCLA central ticket office through www.ticketmaster.com.
Starting May 7, students from any college or university can go to the central ticket office and get one free ticket with a valid student ID card. There is a limit of one per person per ID card.