text:
Kit Contents
|Product number
|Dynabeads® Oligo (dT)25*
|5 ml
|10 ml
|Lysis/Binding Buffer
|30 ml
|60 ml
|Washing Buffer A
|60 ml
|120 ml
|Washing Buffer B
|30 ml
|60 ml
|10 mM Tris-HCl (Elution Buffer)
|15 ml
|15 ml
*Approximately 5 mg/ml, supplied in PBS pH 7.4, containing 0.02% NaN3 as a preservative.
Product # 610.11 provides enough reagents for 20 standard isolations.
Product # 610.12 provides enough reagents for 40 standard isolations.
The suspension of Dynabeads® Oligo (dT)25 and the buffers provided are produced and packed under RNase-free conditions. All kit reagents are of analytical grade and are RNase-free.
Intended Use
This product has been designed for a simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues. The isolated mRNA is suitable for use in all downstream applications.
The isolation protocol relies on base pairing between the polyA residues at the 3' end of most mRNA, and the oligo (dT)25 residues covalently coupled to the surface of the Dynabeads® . Other RNA species lacking a polyA tail will not hybridize to the beads and are readily washed away. RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase, without the use of strong chaotropic agents. The protocol is flexible and can easily be scaled up or down to suit all sample sizes. It has successfully been used in the isolation of mRNA from single cells. The high capture efficiency facilitates detection of mRNA by reverse transcriptase (RT)-PCR from highly specialized cells (e.g. isolated from a heterogeneous sample by immunomagnetic separation). In addition, the protocol has been successfully used to isolate mRNA from a wide variety of tissues of mammalian, fish, amphibian, insect and plant origins.
For many applications elution of the mRNA from the beads is not required as the beads do not interfere with downstream enzymatic reactions. The bead-bound oligo (dT)25 can also function as a primer for RT and synthesis of first-strand cDNA, allowing the construction of solid-phase cDNA libraries and solid-phase RT-PCR.
Binding Capacity
1 mg of beads (200 μl) will bind up to 2 μg of mRNA. A typical mammalian cell contains about 10-30 pg of total RNA, from which 1-5 % is mRNA.
Description of Materials
Characteristics of Dynabeads® Oligo (dT)25
Dynabeads® are uniform, superparamagnetic beads. They are stable in the pH range of 4-13. Do not freeze the Dynabeads® Oligo (dT)25.
Diameter: 2.8 μm ± 0.2 μm (C.V. max 5%)
Surface area: 3-7 m2/g Density: Approx. 1.6 g/cm3
Magnetic mass susceptibility: 120 ± 25 × 10-6 m3/kg
Lysis/Binding Buffer
100 mM Tris-HCl, pH 7.5
500 mM LiCl
10 mM EDTA, pH 8
1% LiDS
5 mM dithiothreitol (DTT)
Washing Buffer A
10 mM Tris-HCl, pH 7.5
0.15 M LiCl
1 mM EDTA
0.1% LiDS
Washing Buffer B
10 mM Tris-HCl, pH 7.5
0.15 M LiCl
1 mM EDTA
10 mM Tris-HCl (Elution Buffer)
10 mM Tris-HCl, pH 7.5
Please note that precipitate may form in the buffers. Dissolve precipitate before use by warming to room temperature and mixing thoroughly.
Additional Material Required
- Magnets: See www.lifetechnologies.com/magnets for magnet recommendations.
- RNase free pipette tips and pipettors.
- RNase free microtubes.
- Mixer allowing both tilting and rotation.
- Heat block and/or incubator at 65-80°C for elution step (if required).
For isolation of mRNA from tissue samples:
- Liquid nitrogen.
- Mechanical or manual tissue grinder.
- Syringe and 21 gauge needle.
- Benchtop microcentrifuge.
Technical Advice
- Keep Dynabeads® Oligo (dT)25 in liquid suspension during storage and all handling steps. Resuspend well before use.
- Work RNase free and wear gloves.
- Bring all buffers, except the 10mM Tris-HCl (Elution Buffer), to room temperature prior to use. The 10mM Tris-HCl buffer should be stored on ice or at 2-8°C prior to use.
- Thorough resuspension of the beads/mRNA complex during washing and complete removal of the washing buffer at each step will prevent carry over of LiDS and other salts to the downstream reaction. Transferring the beads/mRNA complex to new tubes before the last washing step will further reduce LiDS carry over. LiDS is a strong inhibitor of enzymatic reactions.
Protocols included
Preparation of Sample Lysate
The mRNA content of cells and tissues varies greatly depending on the source of the material and RNA expression levels at the time of tissue/ cell harvest. Dynabeads® mRNA DIRECT™ Kit protocols can be scaled up or down to suit specific sample source and quantity. Please see section "Sample Guidelines and Scaling" before preparing the sample, and for recommended bead and buffer volumes (Table 1 and 2).
A) Preparation of Lysate from Solid Plant or Animal Tissue
- Aliquot (weigh) the animal or plant tissue while frozen, to avoid mRNA degradation. Ideally the tissue should be weighed and aliquoted before freezing. Do not exceed the specified amount of tissue, as using too much tissue will reduce the mRNA yield and purity.
- Grind frozen tissue in liquid nitrogen. Work quickly.
- Transfer the frozen powder to the appropriate volume of Lysis/Binding Buffer and homogenize until complete lysis is obtained (approx. 1-2 min). A rapid lysis in the Lysis/Binding Buffer is critical for preventing mRNA degradation.
- A DNA-shear step is advised for samples containing over 500,000 cells. Force the lysate 3-5 times through 21 gauge needle using a 1-2 ml syringe to shear the DNA. The reduction in viscosity should be noticeable. Repeated shearing causes foaming of the lysate due to detergent in the buffer, however, this should not effect the mRNA yield. The foam can be reduced by a 30 second centrifugation. The lysate can be frozen and stored at –80°C for later use.
- Prepare Dynabeads® Oligo (dT)25 as described below, and subsequently proceed with the mRNA isolation in "Direct mRNA Isolation Protocol".
B) Preparation of Lysate from Cultured Cells or Cell Suspension
- Pellet cells by centrifugation (e.g. at 400 g for 8 minutes at 4°C) and wash the pellet by resuspending in phosphate-buffered saline (PBS). Pellet cells by centrifugation again. The cell pellet can be used immediately, or frozen in liquid nitrogen or at –80°C for later use.
- Add the appropriate volume of Lysis/Binding Buffer to either a frozen cell pellet or to a fresh cell pellet. Perform a repeated passage of the solution through a pipette tip to obtain complete lysis. The release of DNA during lysis results in a viscous solution which confirms complete lysis.
- A DNA-shear step is advised for samples containing over 500,000 cells. Force the lysate 3-5 times through a 21 gauge needle using a 1-2 ml syringe to shear the DNA. The reduction in viscosity should be noticeable. Repeated shearing causes foaming of the lysate due to detergent in the buffer, however, this should not effect the mRNA yield. The foam can be reduced by a 30 second centrifugation. The lysate can be frozen and stored at –80°C for later use.
- Prepare Dynabeads® Oligo (dT)25 as described below in section below, and subsequently proceed with the mRNA isolation.
Preparation of Dynabeads® Oligo (dT)25
- Resuspend Dynabeads® Oligo(dT)25 thoroughly before use.
- Transfer the desired volume of beads from the stock tube to a RNase-free 1.5 ml microcentrifuge tube and place the tube on a magnet (e.g. DynaMagTM-2).
- After 30 seconds (or when the suspension is clear), remove the supernatant.
- Remove the tube from the magnet and wash the beads by resuspending in an equivalent volume of fresh Lysis/Binding Buffer. Optional: For very small bead volumes (mini and micro isolations) use 50–100 μl wash volume to ease handling..
- Proceed to section below.
Direct mRNA Isolation Protocol
- Remove the Lysis/Binding Buffer from the pre-washed Dynabeads® Oligo(dT)25 by placing on the magnet for 30 seconds, or until the suspension is clear.
- Remove the microtube from the magnet and add the sample lysate
- Pipette to resuspend the beads completely in the sample lysate. Incubate with continuous mixing (rotating or roller mixer) for 3-5 min. at room temperature to allow the polyA tail of the mRNA to hybridize to the oligo(dT)25 on the beads. Increase the incubation time if the solution is viscous.
- Place the vial on the magnet for 2 min. and remove the supernatant. If the solution is noticeably viscous, increase the time to approx. 10 min.
- Wash the beads/mRNA complex two times with the appropriate volume of Washing Buffer A at room temperature. Use the magnet to separate the beads from the solution between each washing step.
- Wash the beads/mRNA complex once with the appropriate volume of Washing Buffer B at room temperature Use the magnet to separate the beads from the solution.
- If the isolated mRNA is to be used in enzymatic downstream applications (e.g. RT-PCR), one extra wash in Washing Buffer B is recommended. This should be followed by a final wash in the enzymatic buffer to be used (e.g. RT-PCR buffer without the enzyme or primers).
- If elution of mRNA from the beads is desired, add an appropriate volume of 10 mM Tris-HCl (Elution Buffer) and incubate at 65-80°C for 2 min. Immediately place the tube on the magnet, transfer the supernatant containing the mRNA to a new RNase free tube and place this tube on ice.
Note: Perform cDNA synthesis as recommended by the manufacturer of the reverse transcriptase. When using a thermostable reverse transcriptase and the bead-bound oligo (dT) as primer for first strand cDNA synthesis, an initial incubation at 50°C for 5 minutes is necessary before proceeding at the recommended temperature.
Re-use of Dynabeads® Oligo (dT)25 for Large Scale Isolations
Please note that the buffers supplied with the kit (product no. 610.11 and 610.12) may not be sufficient for large scale mRNA isolations. Multiple isolations from the same sample can be performed by re-using Dynabeads® Oligo(dT)25 after mRNA elution. Simply follow the protocol described in section "Direct mRNA Isolation Protocol". After elution of the mRNA, wash the beads once in Lysis/Binding Buffer (section above). Add a new lysate sample to the beads and continue the isolation as usual. Alternatively, washed beads can be re-applied to the same sample lysate until all the mRNA has been captured.
Elimination of rRNA Contamination
In some cases trace amounts of ribosomal RNA have been observed in the mRNA samples. For many applications such as Northern blotting and RT-PCR, trace amounts of rRNA contamination will not interfere with the analysis or interpretation of the results. However, for other applications such as cDNA library construction and microarray analysis, rRNA contamination should be avoided. Ribosomal RNA is effectively eliminated by re-extracting the mRNA from the eluate. Re-use of the same Dynabeads® Oligo(dT)25 used for the original isolation is recommended. If new beads are used, it is recommended that the beads are washed in 50 mM Sodiumpyrophosphate before the isolation of mRNA.
- Follow the isolation protocol (Direct mRNA Isolation Protocol above). Elute the mRNA in 10 mM Tris-HCl (Elution Buffer). Transfer the eluted mRNA to a new tube and place on ice. Do not discard the beads.
- Wash the beads two times in Washing Buffer B.
- Dilute the eluted mRNA in 4 times its volume of Lysis/Binding Buffer (e.g. if the mRNA is eluted in 20 μl, add 80 μl of Lysis/Binding Buffer.)
- Remove the Washing Buffer B from the beads, by placing the tube on the magnet, and add the diluted mRNA Incubate with mixing at room temperature for 3-5 min.
Continue with the isolation protocol ( Direct mRNA Isolation Protocol, starting at step 4.)
Sample Guidelines and Scaling
The following information is intended as a rough guide to the expected total RNA content of selected tissues, as well as appropriate bead and buffer volumes.
Table 1. Estimated total RNA yield from mammalian cells and tissues
|Cell Types & Quantity
|Estimated Total RNA Content (1-5% is mRNA)
|Single mammalian cell
|10-30 pg
|50 mg of muscle tissue
|50-80 μg
|50 mg of liver tissue
|400 μg
|107 cultured fibroblasts
|50-80 μg
|107 cultured epithelial cells
|100-120 μg
Table 2. Recommended volumes of Dynabeads® Oligo(dT)25 and buffers for use with different amounts of starting material
|Plant tissue
|100-400 mg
|20-100 mg
|4-20 mg
|≤ 4 mg
|Animal tissue
|50-200 mg
|10-50 mg
|2-10 mg
|≤ 2 mg
|Cultured cells
|4-20 × 106
|1-4 × 106
|0.15-1 × 106
|≤ 150,000
|Dynabeads® Oligo(dT)25
|1 ml
|250 μl
|50 μl
|10 μl
|Lysis/Binding Buffer
|5 ml
|1250 μl
|300 μl
|300 μl
|Washing Buffer A
|10 ml
|1-2 ml
|600 μl
|600 μl
|Washing Buffer B
|5 ml
|1-1.5 ml
|300 μl
|300 μl
|Tris-HCl (elution is optional)
|50-100 μl
|10-25 μl
|10 μl
|10 μl
Sample Types from which mRNA has been Isolated Using Dynabeads® Oligo (dT)25
Table 3. mRNA DIRECT from animal tissues
|Mouse, Trout
|12, 4
|Brain (cereberal
cortex, preoptic area,
dentate gyrus)
|13, 14, 15
|Organ of Corti and
spiral ganglion
|Guinea pig, rat
|Ear (cochleae)
(paraffin embedded)
|13, 19*
|Kidney (glomerular
|Liver (paraffin embedded)
|Rat, trout, Xenopus
|13, 19*, 4
|Lung (paraffin embedded)
|Rat, trout
|19*, 4
|Nematode (frozen
rehydrated cysts)
|Trout, Xenopus
|Paraffin embedded
lung, liver, gut
|Paraffin embedded
|Skin (dried)
|4, 13
|Schistosoma mansoni
|Whole insect
*Lysis buffer with 4 M urea and 1 % SDS
Table 4. mRNA DIRECT from plant tissues
|Whole plants
|Arabidopsis thaliana
Rice, Oryza sativa
|23, 24, 25, 26
|Epidermal leaf cell
(single cells)
|Maize, tobacco
|Guinea pig, rat
|Guard cell in leaf
(single cells)
Brassica oleracea
|31, 4, 32
29, 27
Brassica oleracea
|Seed aleurone
|31, 4, 35, 36, 32
|Seed endosperm
|35, 36, 32
|Seed embryos
|31, 4, 35, 36, 32
|Maize, tobacco
|29, 28
|Single leaf cells
|Brassica oleracea
|33, 37
|Stolon tips
Table 5. mRNA DIRECT from different types of cells
|Cell type/cell line
|Cervical cancer cells
|38, 39
|Colon carcinoma cell line
|Fibroblast cells line
(ST-1 and SKB-1)
|41, 9
|Fibroblast (D551)
|8, 9
|Fibroblast (RTG-2)
|Endothelial cells
(umbilical cord)
|38, 9
|Hepatocyte cell line
|8, 39
|42, 21
|Langerhans cells
|Lymphoblast B-cell
lines (Reh, Daudi,
|Mamma carcinoma
cells (MCF7)
|38, 39
|Mamma carcinoma
|Pancreas, insulinoma
Rinm5F cells
|Peripheral blood
mononuclear cells
|Peritoneal exudate
|Placental cell line
|38, 39
|T-cells/T-cell clones
|2, 47, 48, 49, 50
|Yeast (Saccharomyces
cerevisiae, Hansenula
|In soil samples
|Yeast (Saccharomyces
Table 6. Direct isolation of viral polyA RNA with Dynabeads® Oligo (dT)25
|Starting Material
|Cells in bronchoalveolar
|Cerebrospinal fluid
|Cell line
|Peripheral blood
mononuclear cells
|54¹ ²
|55¹, 56¹, 54¹ ²
|T-lymphocytes cell line
1) Lysis/binding buffer: 1 M LiCl, 2% SDS, 2xTE, 50 μg tRNA, Vanadyl ribonucleosyl complexes.
2) Lysis/binding buffer: 4 M GTC, 0.5% sarkosyl, 1% DTT, 0.5 M LiCl, 0.1 M Tris pH8.
|Possible Cause
|Suggested Solution
|Clumping of beads during incubation step with sample lysate.
|DNA in the sample lysate has not been completely sheared.
|i) Pipette the solution several times through a 1 ml pipette.
ii) Increase force/number of passages through the needle in future shearing steps.
|mRNA is contaminated with DNA.
|i) Incomplete DNA shearing.
ii) Incomplete removal of sample lysate after hybridization step, and subsequent carry over to wash and elution steps.
iii) Inefficient washing. iv) Incomplete removal of wash buffers. v) Sample-to-beads ratio too high.
|i) Increase the force and /or the number of passages through the needle in the DNA shearing step.
ii) Completely remove the sample lysate after hybridization.
iii) Make sure the beads/mRNA complex is fully resuspended in washing buffer.
iv) Completely remove the sample/washing buffers. v) Dilute sample lysate or increase the amount of beads.
vi) Re-extract the mRNA from the eluate.
|mRNA yield is lower than expected.
|i) Inefficient elution of mRNA from the beads.
ii) Beads-to-sample ratio is too low. iii) Cells/tissue incompletely lysed.
|i) Increase the elution volume/time/temperature or perform the elution step two times, pooling the eluate.
ii) Increase the amount of beads.
iii) Repeat the homogenization step.
|The beads/cDNA complex is clumped and sticking to the tubes after reverse transcription.
|Non-specific electrostatic interactions between the cDNA molecules and the plastic materials of the tubes/pipette tips.
|i) Add BSA (0.2-1.0% final concentration) to the reverse transcription mix before performing the cDNA synthesis. This is to reduce clumping of the beads for a more efficient cDNA synthesis.
Note: use best possible BSA quality.
ii) Where appropriate, add 0.05% Tween-20 to the reaction buffers.
iii) Alternatively, dilute the beads/cDNA solution (after reverse transcription) with an equal volume of the 1 × reverse transcription reaction buffer containing 0.05% Tween-20. Mix by pipetting and transfer the suspension to a new tube. If there are any remaining beads stuck to the tube walls, remove by washing with a fresh aliquot of buffer containing Tween-20. Pool these beads with the original bead suspension. Place the pooled beads on a magnet and remove the supernatant, then wash 2-3 times with the buffer containing Tween-20. Store the solid-phase cDNA library in an appropriate buffer containing 0.05% Tween-20.
|Unable to detect specific mRNA molecules.
|i) The beads-to-sample ratio is too low.
ii) Inappropriate sample volume.
iii) Hybridization time too short.
|i) Increase the amount of beads.
ii) Reduce sample volume/increase sample concentration.
iii) Increase hybridization incubation time to 10-15 min.
Using the appropriate bead-to-sample ratio (volume and concentration) there is no bias in hybridization based on mRNA size. However, with a large excess of mRNA or short incubation time, binding to the beads may be biased towards the short molecules. A similar bias may occur if the sample volumeto- bead ratio is too high.
Invitrogen Dynal® AS complies with the Quality System Standards ISO 9001:2000 and ISO 13485:2003.
This product is stable until the expiry date stated on the label when stored unopened at 2-8°C. Store opened vials at 2-8°C and avoid bacterial contamination. Keep Dynabeads® in liquid suspension during storage and all handling steps, as drying will result in reduced performance. Resuspend well before use.
Technical Support
Please contact Invitrogen Dynal® for further technical support (see contact details). Certificate of
Analysis/Compliance is available upon request.
Warning and Limitations
This product is for research use only. The product is not for use in human diagnostics or therapeutic procedures. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide (NaN3) as a preservative, which is cytotoxic. Avoid pipetting by mouth!
Sodium azide may react with lead and copper plumbing to form highly explosive metal azides. When disposing through plumbing drains, flush with large volumes of water to prevent azide build up. Material Safety Data Sheet (MSDS) is available at http://www.lifetechnologies.com.
- Karrer EE, et al. (1995) In situ isolation of mRNA from individual plant cells: Creation of cell-specific cDNA libraries. Proc Natl Acad Sci USA 92:3814-3818.
- Raineri I, et al. (1991) Improved efficiency for single-sided PCR by creating a reusable pool of first strand cDNA coupled to a solid phase. Nucleic Acids Research 19(14):4010.
- Lambert KN and Williamson VM. (1993) cDNA library construction from small amounts of RNA using paramagnetic beads and PCR. Nucleic Acids Research 21(3):775-776.
- Jakobsen KS, et al. (1994) Direct mRNA isolation using Magnetic Oligo(dT) Beads: A protocol for all types of cell cultures, animal and plant tissues. Advances in Biomagnetic Separation, Ed. Uhlèn M, Hornes E and Olsvik Ø, Eaton Publishing 61-71.
- Rodriguez IR and Chader GJ. (1992) A novel method for the isolation of tissue-specific genes. Nucleic Acids Research 20(13):3528.
- Sharma P, et al. (1993) PCR-based construction of subtractive cDNA library using magnetic beads. BioTechniques 15(4):610-611.
- Schraml P, et al. (1993) cDNA subtraction library construction using a magnet-assisted subtraction technique (MAST). Trends in Genetics 9(3):70-71.
- Aasheim HC, et al. (1994) A simple subtraction method for the isolation of cell-specific genes using magnetic monodisperse polymer particles. Biotechniques 16(4):716-721.
- Coche T, et al. (1994) Generation of an unlimited supply of a subtracted probe using magnetic beads and PCR. Nucleic Acids Research 22(7):1322-1323.
- Borgnes A, et al. Detection of isolated colon carcinoma cells in peripherial blood- or bone marrow mononuclear cell suspensions. Micrometastasis Meeting Munich, June 1996 (Abstract).
- Dazert SB, et al. (2007) Hearing development and spiral ganglion neurite growth in VASP deficient mice. Brain Research 1178:73-82.
- Moyal M, et al. (1992) Mutations in the UL53 gene of SV-1 abolish virus neurovirulence to mice by the intracerebral route of infection. Virus Research 26:99-112.
- Pines G, et al. (1992) Cloning and expression of a rat brain L- glutamate transporter. Nature 360:464-467.
- Alme MN, et al. (2007) Chronic Fluoxetine Treatment Induces Brain Region-Specific Upregulation of Genes Associated with BDNF-Induced Long-Term Potentiation. Neuronal Plasticity:1-9.
- Kim K, et al. (1994) Competitive PCR for quantification of gonadotropin releasing hormone mRNA level in a single micropunch of the rat preoptic area. Mol Cel Endocrinol 97:153-158.
- Winterpacht A, et al. (1994) Alter-native splicing as the result of a type II procollagen gene (COL2A1) mutation in a patient with Kneist Dysplasia. Hum Mol Genet 3(10):1891-1893.
- Safieddine S and Wenthold RJ. (1997) The glutamate receptor subunit d1 is highly expressed in hair cells of the auditory and vestibular systems. J Neurosci 17:7523-7531.
- de Andrès B, et al. (1995) Improved method for mRNA extraction from paraffin-embedded tissues. BioTechniques 18(1):42-44.
- Hengerer B. (1993) A rapid procedure for mRNA extraction from a large number of samples. Biotechniques 14(4):522-524.
- Bicknell G, et al. (1996) Separate extraction of mRNA from glomerular epithelial and mesangial cells. Pathological Society of Great Britain and Ireland, 172nd Meeting.
- Shamsher MK, et al. (1995) Novel mutations in keratin 16 gene underlie focal non-epidermolytic palmoplantar keratoderma (NEPPK) in two families. Hum Mol Genet 4(10):1875-1881.
- Schauder B, et al. (1994) Cloning of a cDNA encoding an ectoenzyme that degrades thyrotropin-releasing hormone. Proc Natl Acad Sci USA 91:9534-9538.
- Schüssler P, et al. (1995) Combined isolation of nucleic acids and protein from small amounts of tissue. Trends in Genetics 11(10):378-379.
- Rubin D. Magnetic Bead Isolation of mRNA from Plant Tissue. In: The Red Book Bulletin, Current Protocols in Molecular Biology, Subscriber's Notebook,Anonymous 1992, p. 1,Suppl.20.
- Ivanov R. et al. (2008) EFFECTOR OF TRANSCRIPTION2 is involved in xylem differentiation and includes a functional DNA single strand cutting domain. Developmental Biology (313):93-106.
- Magrelli A, et al. (1994) Splicing of the rolA transcript of Agrobacterium rhizogenes in Arabidopsis. Science 266:1986-1988.
- Zhu QH, et al. (2007) compact shoot and leafy head 1, a mutation affects leaf initiation and developmental transition in rice (Oryza sativa L.). Plant Cell Reports (26):421-427.
- Hewelt A, et al. (1994) Promoter tagging with a promoterless ipt gene leads to cytokinin-induced phenotypic variability in transgenic tobacco plants: implications of gene dosage effects. Plant Journal 6(6):879- 891.
- Breton C, et al. (1995) PCR-generated cDNA library of transition-stage maize embryos: cloning and expression of calmodulin genes during early embryogenesis. Plant Mol.Biol. 27:105-113.
- Allen RL and Lonsdale DM. (1993) Molecular characterization of one31. Jakobsen KS, et al. (1990) Purification of mRNA directly from crude plant tissues in 15 minutes using oligo dT microspheres. Nucleic Acids Research 18(12):3669.
- Stacy RAP, et al. (1995) Evolution of the group 1 late embryogenesis abundant (Lea) genes: analysis of the Lea B19 gene family in Barley. Plant Mol Biol 28:1039-1054.
- Kumar V and Trick M. (1994) Expression of the S-locus receptor kinase multigene family in Brassica oleracea. Plant Journal 6(6):807-813.
- Bachem CWB, et al. (1994) Antisense expression of polyphenol oxidase genes inhibits enzymatic browning in potato tubers. Biotechnology 12:1101-1105.
- Espelund M, et al. (1992) Late embryogenesis-abundant genes encoding proteins with different numbers of hydrophilic repeats are regulated differentially by abscisic acid and osmotic stress. Plant Journal 2(2):241-252.
- Aalen R, et al. (1994) Transcripts encoding oleosin and a dormancy related protein are present in both the aleurone layer and the embryo of developing barley (Hordeum vulgare L.) seeds. Plant Journal 5(3):385-396.
- Giranton JL, et al. (1995) The S locus receptor kinase gene encodes a soluble glycoprotein corresponding t the SRK extracellular domain in Brassica oleracea. Plant Journal 8(6):827-834.
- Larsen F, et al. (1993) A tight cluster of five unrelated human genes on chromosome 16q22.1. Hum Mol Genet 2(10):1589-1595.
- Luna L, et al. (1994) Molecular cloning of a putative novel human bZIP transcription factor on chromosome m17q22. Genomics 22:553-562.
- Maas RA, et al. (1995) Immuno-magnetic purification of human breast carcinoma cells allows tumor-specific detection of multidrug resistance gene-1 mRNA by reverse transcriptase polymerase chain reaction in fine needle aspirates. Lab Invest 72(6):760-764.
- Slupphaug G, et al. (1991) Cell cycle regulation and in vitro hybrid arrest analysis of the major human uracil-DNA glycosylase. Nucleic Acids Research 19:5131-5137.
- Sprecher E and Becker Y. (1992) Detection of IL-1, TNF- and IL-6 gene transcription by the polymerase chain reaction in keratinocytes: Langerhans cells and peritoneal exudate cells during infection with herpes simplex virus-1. Arch Virology 126:253-269.
- Day S and Ross J. (1995) mRNA based method for typing the HLA-C locus gene by automated sequencing based typing. Eur J Immunogenet 23(1):92.
- Osnes LTN, et al. (1995) Lipopolysaccharide activation of human monocytes mediated by CD14, results in a coordinated synthesis of tissue factor TNF-alpha and IL-6. J Endotoxin Res 2:27-35.
- Bristulf J, et al. (1994) Interleukin-1 stimulates the expression of type I and type II interleukin-1 receptors in the rat insulinoma cell line Rinm5f; sequencing of a rat type II interleukin-1 receptor cDNA. Eur Cytokine Netw 5(3):319-330.
- Thanhäuser A, et al. (1993) Pentoxifylline: a potent inhibitor of IL-1 and IFNgamma biosynthesis and BCG-induced cytotoxicity. Immunology 80:151-156.
- Raineri I and Senn HP. (1992) HIV-1 promotor insertion revealed by selective detection of chimeric provirus-host gene transcripts. Nucleic Acids Research 20:6261-6266.
- Obata F, et al. (1993) A single universal primer for the T-cell receptor (TCR) variable genes enables enzymatic amplification and direct sequencing of TCR cDNA of various T-cell clones. Human Immunology 36:163-167.
- Spurkland A. (1992) Magnetic isolation of mRNA for in vitro amplification. Trends in Genetics 8(7):225-226. of the maize poly-galacturonase gene family members which are expressed during late pollen development. Plant Journal 3(2):261- 271.
- Murtagh JJ, et al. (1994) Alternative splicing of the guanine nucleotidebinding regulatory protein Goa generates four distinct mRNAs. Nucleic Acids Res. 22(5):842-849.
- Tebbe CC, et al. (1995) Direct detection of recombinant gene expression by two genetically engineered Yeasts in soil on the transcriptional and translational levels. Appl Environ Microbiology 61(12):4296-4303.
- Faulkner JDB and Minton NP. (1993) Rapid small-scale isolation of mRNA from whole yeast cells. Biotechniques 14(5):718-720.
- Chiodi F, et al. (1992) Human immuno-deficiency virus type 1 is present in the cerebrospinal fluid of a majority of infected individuals. J Clin Microbiol 30(7):1768-1771.
- Meijer A, et al. (1995) Development of molecular methods for detection and epidemiological investigation of HIV-1, HIV-2 and HTLVI/II infections. Report 118504 001 National Inst. Publ. Health and Env. Protection Bilthoven, The Netherlands.
- Albert JA, et al. (1992) Persistence of azidothymidine-resistant human immunodeficiency virus type 1 RNA genotypes in posttreatment sera. J Virol 66(9):5627-5630.
- Scarlatti G, et al. (1993) Comparison of variable region 3 sequences of human immunodeficiency virus type 1 from infected children with the RNA and DNA sequences of the virus population of their mothers. Proc Natl Acad Sci USA 90:1721-1725.
- Sin Y, et al. (2008) Skin bradykinin-related peptides (BRPs) and their biosynthetic precursors (kininogens): Comparisons between various taxa of Chinese and North American raid frogs. Peptides 29:393-403.
- Lu X, et al. (2007) A comparison of CFU-GM, BFU-E and endothelial progenitor cells using ex vivo expansion of selected cord blood CD133+ and CD34+ cells. Cytotherapy 9:292 - 300.
- Silva J, et al. (2007) Circulating Bmi-1 mRNA as a possible prognostic factor for advanced breast cancer patients. Breast Cancer Research 9:1-9.
- Jost R, et al. (2007) Magnetic quantitative reverse transcription PCR: A high-throughput method for mRNA extraction and quantitative reverse transcription PCR. BioTechniques 43:206-211.


text:
About Eric N.
Birthday: July 6, 2015
Sex: Male
Fun Fact: Favorite food is potatoes
How to sponsor:
- Click on the "Sponsor Today!" button below to open a new window.
- Choose "Monthly Recurring Donation" and enter $50 into the box.
- Enter your credit card and address information.
- In the comment box write the name of the child you are sponsoring.

subset: commoncrawl


text:
Delivers Strong Growth in Revenue & Profits, $220 Million in Free Cash Flow
Milpitas, CA, October 20, 2009 - SanDisk Corporation (NASDAQ:SNDK), the global leader in flash memory cards, today announced results for the third quarter ended September 27, 2009. Total third quarter revenue of $935 million increased 14% on a year-over-year basis and increased 28% on a sequential basis. Net income, in accordance with U.S. Generally Accepted Accounting Principles (GAAP), was $231 million, or $0.99 per diluted share, compared to GAAP net loss of ($166) million or ($0.74) per share in the third quarter of 2008 and GAAP net income of $53 million, or $0.23 per diluted share in the second quarter of 2009.
On a non-GAAP basis, which excludes the impact of acquisition-related charges, share-based compensation expense, and non-cash economic interest expense associated with the cash-settled convertible note, third-quarter net income was $176 million, or $0.75 per diluted share, compared to a net loss of ($132) million or ($0.59) per share in the third quarter of 2008 and net income of $83 million or $0.36 per diluted share in the second quarter of 2009. For reconciliation of non-GAAP to GAAP results, see accompanying financial tables and footnotes.
"Our outstanding Q3 results reflect major gains in our OEM business with strong orders continuing into Q4. Product gross margin improved dramatically, driven by favorable pricing and strong product cost reductions. We are encouraged by improved industry fundamentals and our increasingly diversified global markets, which bode well for further growth in Q4 and in 2010," said Eli Harari, Chairman and CEO, SanDisk.
SanDisk's third quarter 2009 conference call is scheduled for 2:00 P.M., Pacific Time, Tuesday, October 20, 2009, with SanDisk CEO Eli Harari joining the call from SanDisk's Shanghai facility. The conference call will be webcast and can be accessed live, and throughout the quarter, at SanDisk's website at http://www.sandisk.com/IR. To participate in the call via telephone, the dial-in number is 913-312-0702 and the dial-in password is 8643791. A copy of this press release will be furnished to the Securities and Exchange Commission on a current report on Form 8-K and will be posted to our website prior to the conference call.
1 1 GB = 1 billion bytes.
2 Based on internal testing; performance may vary depending upon host device.
SanDisk Corporation President and Chief Operating Officer, Sanjay Mehrotra, is scheduled to appear on CNBC's "Closing Bell with Maria Bartiromo," on October 20, 2009, at approximately 1:10 P.M., Pacific Time.
SanDisk Corporation Executive Vice-President Administration and Chief Financial Officer, Judy Bruner, is scheduled to appear on Bloomberg Television on October 21, 2009, at approximately 6:35 A.M., Pacific Time.
A complete reconciliation between GAAP and non-GAAP information referred to in this release is provided in the attached tables.
This news release contains certain forward-looking statements, including statements about our business prospects and outlook, our expectations for the fourth fiscal quarter of 2009, and fiscal 2010, and our expectations regarding our business, including our OEM business, that are based on our current expectations and involve numerous risks and uncertainties that may cause these forward-looking statements to be inaccurate and may significantly and adversely affect our business, financial condition and results of operations. Risks that may cause these forward-looking statements to be inaccurate include among others:
Future results may differ materially from those previously reported. We do not intend to update the information contained in this release.
SanDisk Corporation, the inventor and world's largest supplier of flash storage cards, is a global leader in flash memory - from research, manufacturing and product design to consumer branding and retail distribution. SanDisk's product portfolio includes flash memory cards for mobile phones, digital cameras and camcorders, digital audio/video players, USB flash drives for consumers and the enterprise, embedded memory for mobile devices, and solid state drives for computers. SanDisk (www.sandisk.com/corporate) is a Silicon Valley-based S&P 500 company with more than half its sales outside the United States.
SanDisk, the SanDisk logo, Sansa, CompactFlash and SanDisk Extreme are trademarks of SanDisk Corporation, registered in the United States and other countries. slotRadio is a trademark of SanDisk Corporation. slotMusic is a trademark of SLOTmedia Group LLC. SDHC and microSD are trademarks of SD-3C LLC. Memory Stick PRO Duo is a trademark of Sony Corporation. Other brand names mentioned herein are for identification purposes only and may be the trademarks of their respective holder(s).
SanDisk Supporting Documents (.pdf)
Investor Contact:
Jay Iyer
(408) 801-2067
Media Contact:
Ryan Donovan
(408) 801-2857


text:
Start entering a first or last name to find someone.
Today we live in a state of constant change. It is a technology-rich world, where communication is instant and information is immediately accessible. The way we interact with each other personally, socially, and at work has changed forever. Knowledge is growing and information is changing extremely quickly, creating new possibilities. This is the world our students are entering.


text:
Color Me Floral: Summer Orange
Color Me Floral: Summer Orange
from 25.00
Museum-quality poster made on thick, durable, matte paper - printed on archival, acid-free paper.
Available in 8x10in and 16x20in sizes.
Add To Cart
Our favorites for your walls. Museum-quality posters made on thick, durable, matte paper. All prints arrive rolled and unframed.
All prints ship internationally (although shipping/delivery times are a bit longer than for U.S. shipments).


text:
We uncovered more than a dozen of examples of ESPN senior writer Lynn Hoppes copy-and-pasting from Wikipedia and he was not fired. Instead ESPN gave him a slap on the wrist, and he hasn't written anything since—maybe he was suspended, or told to keep a low profile for a little while.
In any event, whatever calculus ESPN has dreamed up to deem Hoppes's act an example of "journalistic laziness" but nothing more, Bleacher Report would like to remind you that there would have been far greater consequences at its site:
This is the sort of thing that will get you fired from B/R if you're an employee, or your publishing privileges removed permanently if you're in the writing program. It's fine to use Wikipedia for background material, but you have to follow the information back to the primary source that Wikipedia cites and attribute that if you use it. And you can't just copy the text.
If anyone at ESPN would like to consult Bleacher Report's standards-and-practices guides, they have lots of links on how best to handle a situation like this.


text:
Detroit EM to strip council president's pay, title
DETROIT (AP) — State-appointed emergency manager Kevyn Orr has ordered his legal staff to prepare an order that will strip the pay and authority from AWOL Detroit City Council President Charles Pugh.
Pugh has been a no-show at recent council meetings and failed to acknowledge by a 5 p.m. Wednesday deadline that he would be returning to work, Orr spokesman Bill Nowling said.
Orr will review the completed order and could sign it Thursday, Nowling said.
Orr is operating under Michigan's emergency manager law. He can't fire Detroit elected leaders, but does control their pay and responsibilities. Pugh earns about $76,000 per year.
His request for a three- to four-week medical leave was denied earlier this week by Orr.
The City Council's powers have been reduced since Orr was hired in March to fix a budget deficit barreling toward $380 million and about $17 billion in city debt. He has control over all matters involving money.
The Associated Press left messages Wednesday seeking comment from Pugh, who also is the subject of a complaint the Detroit Public Schools received from the parent of a recent graduate.
The mother, whose name was not released by the district, came forward earlier this month with concerns involving the 41-year-old Pugh and a mentoring program at Douglass Academy for Young Men, according to district spokesman Steve Wasko.
The complaint involved cash and gifts the teenager received while part of the program.
"At the time, the school reached out to the parent who stated that she would prefer to handle the matter personally," Wasko said Wednesday in a statement. "The school has since contacted the parent again in writing seeking to resolve the concerns, and is awaiting a response."
The district will review the leadership mentoring program at Douglass Academy, he added.
Detroit area attorney Ivan Land said he represents the teen's mother and told The Detroit News that the gifts included a $350 cellphone and prom clothing. The teen also received $160 in cash from Pugh, Land said.
Pugh, a former TV anchor, resigned in 2009 from Detroit's Fox Network affiliate and a local radio station to run for council. He became council president after receiving the most votes in that year's general election.
"I have a mandate from the people of Detroit," Pugh said after the election. "The people say they trust me and believe in me, and are looking forward to what new things I bring to the table. We need some new leadership, better collaboration and better cooperation."
Earlier this year, Pugh said he would not seek re-election.
If Pugh steps down, only six council members will remain to conduct city business. Kwame Kenyatta resigned last week and City Council President Pro Tem Gary Brown moves July 1 to Orr's staff.
Detroit City Clerk Janice Winfrey said the council can continue to operate as long as there are enough members to make a quorum, but the loss of members will make it difficult for the smaller council committees to meet.
News from The Associated Press, and a taste of the great journalism produced by AP members and customers. Managed 24/7 by these editors: apne.ws/APSocial Read more ›


text:
Kia Sorento LX Sangria Clean CARFAX. CARFAX One-Owner. Odometer is 9951 miles below market average!
Certified Pre-Owned 2019 KiaSorento 3.3L LX SUV
- Stock: KG560060
13 views in the past 7 days
- Odometer
- 17,888 miles
- Fuel Economy
- 19.0/24.0 mpg City/Hwy
- Exterior Color
- Passion Red
- Interior Color
- Stone Beige
- Body/Seating
- SUV/7 seats
- Transmission
- 8 speed automatic
- Drivetrain
- all-wheel drive
- Engine
- 3.3L V-6 cyl
Kia Sorento
Highlighted Features
- Blind spot sensor
- Automatic temperature control
- Wireless phone connectivity
- Exterior parking camera rear
- Front dual zone A/C
- 3rd row seats
- Split folding rear seat
- Remote keyless entry
Detailed Specifications
Dealer Notes
KBB.com Consumer Reviews
- 5.0
A great car for the price
By DB on Friday, May 24, 2019I have driven high end cars all of my life and my Sorento is one of the absolute best I have ever owned. Fit and finish, inside and out, is perfect, and gas mileage has exceeded, by far, what I was told to expect. I feel sorry for those consumers whose egos won't let them own a Kia. The are missing out on owning a very high quality car
- 4.0
Awesome car if you pick the right one
By NC on Monday, August 19, 20192019 Kia sorento L The car is beautiful inside and out Buying this car need to test drive it observe everything from exterior, interior and under the hood, listen to the engine and visual inspect because any brand cars are some of them are defective so it's better to test drive it and inspect before buying it, after all of that regular maintenance need to check after the job of the mechanic because some mechanics are tricky or abusive or just dont care, always inspect your car after going for service you cant trust 100% the mechanic unless they're friend or family. Asian cars always last long 150,000 to 300,000 or more plus good news sorento is 10years warranty plus if you pick some basic to Platinum plan for maintenance.
- 5.0
Our Second Sorento
By jsh1120 on Sunday, October 28, 2018Purchased a 2018 Sorento SX-L in January 2018. We looked at a variety of midsize crossovers and seriously considered the Ford Edge, Toyota Highlander, and Mazda CX-9 before trading our 2012 Sorento. Several factors went into our decision. An important factor was our 70K plus trouble free miles in our 2012 Sorento. Never so much as a single "check engine" light nor any other issue. Like most Kia owners (as reported in Consumer Reports) we found the Sorento to be ultra-reliable. Along with the superior warranties offered by Kia, it gave us confidence in the 2018 model. Otherwise, the size of the Sorento was a major point in its favor. Its overall length is virtually the same as the Ford Edge but the KIA has usable third row seating while the Edge does not. It is almost a foot shorter than the CX-9 but has more passenger and cargo room! In short, for our mission (suburban traffic and people hauling) the Sorento is better suited and more maneuverable than the other crossovers we considered. We chose the top trim of the Sorento, the SX-L. We found it superior to the top trims of the Highlander and Edge and at least equal to that of the Signature trim of the CX-9. (The Sorento has a panoramic sunroof and third row HVAC controls; the CX-9 does not.) In terms of overall performance the Ford Edge Sport with a turbo V6 was a clear winner. If 0-60 time had been a priority the Ford would have had an "edge." (Sorry.) The others we considered were virtually identical. But the Sorento and Highlander have naturally aspirated V6 engines compared to the turbo 4 of the Mazda and the Ford's turbo V6. Turbocharging is an appealing technology in terms of performance but in terms of long-term reliability and smooth, quiet operation naturally aspirated V6 engines are simply better. After 10,000 miles we're extremely satisfied with the Sorento. Trouble free, comfortable, feature rich, and maneuverable in the jungle of suburban traffic and parking lots, it's all we could ask for.


text:
886 0359
Armedica AM-BA 150 Treatment Table
Heavy duty steel tube Hi-Lo control allows adjustment of the table height while keeping both hands on the patient and both feet on the floor. The control is designed to allow easy access from anywhere around the table without disrupting the treatment.
Standard Features:
Sort By:


text:
Freddie Eckstine: The Legacy Lives!
Freddie Eckstine: "My hat is off to Wynton Marsalis and his family for their considerable efforts to keep jazz alive. New Orleans was a great loss for the jazz world. However, bigger than jazz or any music, is the crushing blow those folks down there suffered...."
The magic of Billy Eckstine shines on through his son, Freddie Eckstine, whose amazing talent has enabled him to carve out his own star power while still paying tribute to the memory of his father.Â
Freddie who hails from Chicago now basks in the bright lights and glitz and glamour of the Las Vegas hotels, casinos, and boardwalks. Indeed, he is on a "Sentimental Journeyâ€? that assures the 'Eckstine' Jazz legacy lives on.Â
There is no doubt that Freddie is Mr. 'B's' son. The gene pool kicked in and brought forth a matching set in appearance and in vocal similarity, great news for Billy Eckstine fans. Freddie Eckstine's ability to croon romantic jazz melodies that venerate his dad, combined with his own mastery over soul, funk, blues, gospel, and R&B music, make him unforgettable.Â
His versatility places him among the premiere entertainers on the Vegas strip.  He's headlined at The Sahara, The Sun Coast Hotel and Casino, The Orleans, Sam's Town and the Boardwalk as the star of "Sentimental Journey.� His sold out performances pay tribute to his father, Billy Eckstine and the Divine One, Ms. Sarah Vaughn.
More recently, Freddie, who is occasionally referred to as the Scion of Soul, due to his genuine charm, deep velvety bass and baritone pipes, packaged his soulful sound via his CD "St. Croix.� He will bring that same jazzy soul to Helsinki, Finland when he appears as one of the main artists at Helsinki's 21st Annual Music Festival, April Jazz Espoo, via the invitation of Martii Lappalainen, founder and leader of the Espoo Big Band.
"I am so excited about that. It's my first headline jazz festival. Although, it's still in the ground breaking stages, I am hoping to record an album before the festival starts so I can present it to the folks there� stated Eckstine. "Believe it or not, the Finnish are very much into jazz. In fact, they are students of jazz. They cannot only tell good music when they hear it, they can recite at a moments notice some of the jazz greats and their contributions to American jazz. I would like to combine several genres of blues, funk, and R&B melodies into a big band jazz format for the festival� said Freddie.
"We are hoping that this thing launches into a European tour which includes Sweden. Swedes are great devotees of jazz. There was a period in America, around the time that the Beatles came over, when music changed and many jazz artists found themselves having to go abroad in an attempt to survive. In fact, one of those musicians was my father, "Mr. B.â€?Â
He took Sarah Vaughn with him and right behind him was Count Basie, Duke Ellington, and all the greats. They found a wonderful home there. I am hoping to achieve the same goal. You know there will be some that say America had a home for musicians back then, but the truth of the matter is America didn't. And in some ways, that holds true to this day� said the man known as the Ambassador of the Blues.
"My hat is off to Wynton Marsalis and his family for their considerable efforts to keep jazz alive. New Orleans was a great loss for the jazz world. However, bigger than jazz or any music, is the crushing blow those folks down there suffered. My prayers remain constant for the people of New Orleans in their valiant effort to rebuild their city� remarked the multitalented performer.
Freddie is a big band leader. "I was fortunate enough to be invited as one of the headliners at the Joe Williams Foundation Annual Benefit where I was able to use a lot of big band charts. We experimented with a few things and it turned out to be a great success. I hope to do the same next year� said Freddie of his love of big band music.
Billie Eckstine was well known for supporting young musicians, he was very helpful to the young Miles Davis, Gene Ammons and Leo Parker when they first started out. In fact, they became affectionately known as "B's babies.' "Mr. "B� was very instrumental in the start of Sarah Vaughn's career, Ruth Brown, and even comedians Tom Dreesen, and Redd Foxx. My father loved a good joke. He helped many people and that has rubbed off on me too� remarked Freddie.
"You know Ruth Brown is a wonderful lady and a great mentor. She is very uplifting and encouraging. You know sometimes due to her legs she has to sit down while performing. I just look at that as her sitting on her throne with all her subjects around her while she entertains with her whole heart� claimed Freddie. "There was a time Dad took Ruth, the Four Tops, and Count Basie on tour. They experienced a lot of hate and racism on the road. However it lent itself to their fortitude and gave them a strong foundation so they could weather all the storms of prejudice. They stuck together and made it through� recalled Freddie proudly of his father's bravery. "I wish there were a museum that paid tribute to all those pioneers who made major contributions to American music, oftentimes under extreme conditions� remarked Eckstine about America's shameful past.
Freddie was reminded of a family tale or two. "My grandmother was the understudy for her girl friends Marian Anderson and Mahalia Jackson. I recall a special moment when I observed these great ladies singing acappella in the kitchen together while preparing pound cake. My childhood experiences were rich in music. At first I resisted but finally gave into it. Now, here I am today paying tribute to the wonderful Mr. 'B.� I have been accused of riding on my father's coattails. However, I will tell anyone: 'No, I am not riding on his coattails. I am standing on his shoulders. And, they are very broad shoulders, indeed.'�
To book Freddie Eckstine contact firstname.lastname@example.com.
To subscribe to New York's favorite Pan-African weekly investigative newspaper please click on "subscribe� on the homepage or call (212) 481-7745. For advertisements or to send us a news tip contact email@example.com "Speaking Truth To Empower,� is our motto.
Latest comments
- Ann GarrisonFebruary 22,2018 @ 08:03 PM
I think you have "Filtered HTML" turned on here. If you switch to "Full HTML," it will probably...
googletag.pubads().definePassback('6785150/BENN/BlackStarNews', [[300,250]]).setTargeting('fold',['BTF']).display();


text:
Since the resignation of Pope Benedict, we have been treated to the truly tawdry spectacle of life in the Vatican at this time in history.
There are stories of Papal shoes, rings, palaces and castles. We see pictures of old men dressed in ecclesiastical finery sitting in palatial rooms at the Vatican. There are stories of corruption and jockeying for position among the cardinals. We are informed of the number old men who will have voting rights at the conclave called to elect the new pope. For some reason that defies logic a disproportionate number are Italian. Many Americans are deeply offended by the presence of Cardinal Roger Mahony of Los Angeles. Of course the laity has no role to play. We are expected to sit back and watch our church implode.
Clearly, major changes are needed. We have experienced significant changes in the liturgy over the years. Now we need to implement major changes in church governance. Major reforms will be required if the Catholic Church is to continue to have a role in the world. I propose that the College of Cardinals be suppressed. It should be replaced by a College of Bishops and a College of the Laity. Both bodies would be elected to office on a regional basis. The College of the Laity would not simply be an advisory board of fat cats who win the support of bishops. This would be a legislative body of serious members of the laity who will represent those currently with no voice.
I realize that some will find my proposal to be extremely audacious as we are conditioned to believe that the role of the laity is akin to that of the old fashioned view of children: we should be seen, we should contribute but we should not expect to be heard. Sacred scripture does not assign this passive role to the laity nor does it discuss cardinals and what a cardinal should wear. I believe that the laity need to feel a sense of empowerment. Many other members of the laity will have other recommendations which may be much better than mine.
We can be complacent no longer. We can no longer assume that the bishops are working in the best interests of the church when it is very clear that so many are operating out of self interest. Those of us who are insisting on significant substantive change will not be silenced and we will not go away.
Edward McCarey McDonnell, Baltimore


text:
Cost: $23 Drop in [or get your first week free!]
Through a series of long held postures, CoreRestore focuses on gently stretching and healing the connective tissue that form our joints rather than on muscular engagement. CoreRestore requires minimal alignment and no muscular engagement to access the benefits of the pose. It is in this aspect that CoreRestore evolves into an individualized practice which takes into account personal anatomical structure. CoreRestore has the same goals and objectives as all other types of yoga; however, it directs the benefits and stimulation deeper than the superficial or muscular tissues. CoreRestore impacts connective tissues such as the ligaments, bones, and even the joints of the body, specifically the connective tissues of the hips, lower spine and pelvis. Suitable for all levels of students, CoreRestore is a perfect complement to the dynamic and muscular styles of yoga that emphasize internal heat and the lengthening and contracting of our muscles. Our mission is to provide more balance in our class options for our students to choose from. Benefits of CoreRestore include: A slow, recuperative practice balances CorePower's current yoga offerings. Less effort yields a deep, relaxed state. Targets connective tissue (ligaments, bones, joints, fascia) versus muscle. A calm mind and body is the same goal for all CorePower classes.


text:
Political insiders know that the Alfred E. Smith Dinner strives to honor decades of civic and religious traditions. In election years, it's a tradition that the presidential candidates appear -- wearing formal, white-tie attire -- and satirize their own public images, while also aiming a few gentle shots at their opponent and the ranks of elite journalists in attendance.
Thus, Republican standard-bearer Mitt Romney, with a nod to his Mormon fuddy-duddy reputation, reminded the audience of wine-sipping socialites that, "Usually when I get invited to gatherings like this, it's just to be the designated driver."
Noting that this campaign has not, journalistically speaking, unfolded on a level playing field, he added: "I've already seen early reports from tonight's dinner, headline -- 'Obama Embraced by Catholics. Romney Dines with Rich People.' "
In response, the president poked fun at his own complex and, for some, controversial religious and family background by noting that, like Romney, he has a rather unusual name. "Actually, Mitt is his middle name. I wish I could use my middle name," said Barack Hussein Obama.
But, yes, there is the issue of the Romney family's wealth. "Earlier today, I went shopping at some stores in Midtown," quipped Obama. "I understand Governor Romney went shopping FOR some stores in Midtown."
It is a tradition, of course, that the jokes grab the headlines after this unique, YouTube-friendly scene at the Waldorf-Astoria Hotel on Park Avenue.
But it is also a tradition that this dinner has, throughout its 67-year history, been a crucial fundraiser for charities linked to the Catholic Archdiocese of New York, netting about $5 million this year. Thus, the Catholic shepherd of New York City speaks last and, literally, offers his benediction on this salute to lighthearted, generous public discourse in the tense battlefield that is national politics.
The stakes were especially high this year since Cardinal Timothy Dolan faced withering criticism from Catholic conservatives for extending the traditional invitation to the president -- because Obama has repeatedly clashed with the church over issues related to abortion, same-sex marriage and religious freedom.
The cardinal joined in the humorous repartee -- at one point noting that he couldn't read the greeting sent by Pope Benedict XVI because it was written in Latin -- but turned serious in his final prayer. He reminded the audience that the dinner honored Smith as the first Catholic selected as the presidential nominee of a major party, but also as the "happy warrior" who tirelessly fought to help the poor, the powerless and other forgotten Americans.
"Here we are, in an atmosphere of civility and humor … loving a country which considers religious liberty our first and most cherished freedom, convinced that faith is not just limited to an hour of Sabbath worship, but affects everything we do and dream," said Dolan, who also serves as president of the U.S. Conference of Catholic Bishops.
The purpose of the event, he added, was to "reverently" recall a "man of deep Catholic faith and ringing patriotism, who had a tear in his Irish eyes for what we would call, the 'uns' -- the un-employed, the un-insured, the un-wanted, the un-wed mother and her innocent, fragile un-born baby in her womb, the un-documented, the un-housed, the un-healthy, the un-fed, the under-educated.
"Government, Al Smith believed, should be on the side of these 'uns,' but a government partnering with family, church, parish, neighborhood, organizations and community, never intruding or opposing, since, when all is said and done, it's in God we trust, not, ultimately, in government or politics."
While Dolan is known for his boisterous wit, this final litany was clearly the big idea he wanted to communicate to both candidates and to all who were present, said Father James Martin, author of "Between Heaven and Mirth: Why Joy, Humor, and Laughter Are at the Heart of the Spiritual Life."
"It was very moving, and very Catholic, because he refused to narrow the Gospel down to one or two issues," said Martin, who attended the dinner. "He reminded everyone of the sacred dignity of all human life, not just in the womb, but also not just in the slums. …
"There are Catholics these days, on the left and on the right, who don't want to be reminded of both sides of that equation. What the cardinal did was honor our Catholic tradition -- all of it."


text: The Buncombe County Fire Marshal's office is located in the Emergency Operations Center at 164 Erwin Hills Road in Asheville. The Fire Marshal's Office is responsible for the enforcement of the fire code and minimum housing code for Buncombe County. In addition, the Fire Marshal's office makes regular inspections of businesses, investigates fires, and conducts fire prevention programs throughout the year on an as-requested basis. Information and services may be obtained by calling (828) 250-6620. The following forms are available to download: Buncombe County Fire Prevention Ordinance New Commercial Building Requirements Rental Housing Ordinance Rental Housing Inspection Youth Firesetting Prevention and Intervention Program


text:
Julian_M necesitaba un nuevo diseño de logotipo y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 671 diseños de 132 diseñadores freelance.
Redpalm Technology Services
Redpalm Technology Services is a soon to be launched IT Sales Company that provides business customers with a range of IT Products, we will specialise in supplying trusted networking brands, such as Cisco, HP, Juniper, Vmware, Citrix, Netgear, 3Com, IBM etc.
Will also sell all types of Hardware and Software from all the major business IT brands; Microsoft, Symantec, Lenovo, Acer, Fujitsu-Siemens, Brother, OKI, Epson etc. Redpalm Technology Services is a "one-stop-shop" IT supplier that can cater to any business IT requirements.
Typical competitors are:
- http://www.kelway.co.uk
- http://www.misco.co.uk
- http://www.uk.insight.com
- http://www.caeuk.com
- http://www.equanet.co.uk
- http://www.pcwb.co.uk
- http://www.probrand.co.uk
- http://www.2e2.com
- http://www.uk.logicalis.com
- http://www.hardware.com
- http://www.morecomputers.com
- http://www.trusthardware.co.uk
- http://www.dabs.com
- http://www.overstock.com
- http://www.computacenter.com
- http://www.scc.com
Business customers looking to buy IT equipment. Individuals will be IT managers and directors, admin staff, company purchasing staff etc.
Customers range from small to medium businesses to large enterprises.
We are selling business to business, so we want to retain an element of corporate branding, but we also want to appear modern and fresh. Business websites and the language used has become less serious in past few years and we want to create a website that is fresh in it's styling and without long, boring business waffle!
The theme throughout our website and marketing strategy is one of "keep it simple". Effective, no-nonsense, and conscise. We will attempt to use 1 word where other companies use 10.
We want a logo that we can build the site around and one that matches the above aims. To achieve this we are looking for;
- A striking red colour scheme (some examples of the colour red we like are http://www.red-design.co.uk, http://www.veolia.co.uk, http://www.redmagazine.co.uk)
- A logo that symbolises the name, we would like to see a red palm tree or palm leaf.
- Redpalm is the main name, with technology services in smaller writing.
- A font that is unique, as the word is short we would love to see a completely original font style if possible.
- The font needs to match the logo in style, think VODAFONE. the logo matches the font perfectly
- Simple works for us, we want it to look striking on a White background.
- A WEB 2.0 look and feel.
- Use metallic 3d effects
- Over-complicate things
Vector graphics please! We will communicate regularly with all designers to give constructive feedback.
There is lots more direct work available to designers we like.
Descubre cómo funciona un concurso de diseño u obtén más información sobre nuestros servicios de diseño de logo.


text:
AXIS Camera Station S9002 Desktop Terminal
Effective 4K surveillance for server installations
Discontinued product. Supported until 2023-11-28.After this date, please see the support documentation.
As long as the product is within the warranty period you will be supported.
Recommended replacement: AXIS S9002 Mk II
Note: Check the recommended replacement product data sheet for full specification
- Ease of installation
- AXIS Camera Station client included
- Validated high performing hardware
- Up to three 4K streams
- Support for four monitors
AXIS Camera Station S9002 Desktop Terminal is a complement to AXIS S1032 or AXIS S1048 recorders to enable quick set-up of a surveillance workstation. The terminal is preloaded with an AXIS Camera Station client and preconfigured to minimize installation time.
Out-of-the-box ready
AXIS S9002 is designed for quick installation. The terminal is preloaded with all necessary software including AXIS Camera Station video management software client to enable quick connection to the server. System settings such as camera and storage configurations are preconfigured to further simplify system setup. AXIS S9002 supports four monitors for optimal overview. International keyboard and computer mouse are included.
Maximum reliability
Hardware components such as hard-discs and graphic card as well as storage capacity are tested and validated to meet the demands for reliable high definition surveillance. To further safeguard system uptime. The product includes three-year hardware warranty with on-site hardware replacement service.
AXIS Site Designer
AXIS Site Designer makes it easy to select cameras, accessories and find the right AXIS Camera Station recorder.AXIS Site Designer
Get to know Axis mid-size solutions
Learn how simple it is to protect a mid-size business with a complete, end-to-end security solution from Axis Communications.Free online course to learn more


text:
Guantánamo "closer" is reassigned, office closed
The State Department has reassigned its special envoy for closing the U.S. prison at Guantánamo Bay, Cuba, in another step away from one of President Barack Obama's first campaign promises.
Ambassador Daniel Fried is starting this week as the department's sanctions coordinator, according to an internal notice, focusing on governments like Iran and Syria.
And no one is replacing Fried as lead diplomat to persuade countries to resettle Guantánamo inmates approved for release. Instead, those responsibilities will now transfer to the department's legal office.
The reduced diplomatic effort comes as a military tribunal holds more hearings into the case of alleged 9/11 mastermind Khalid Sheik Mohammed and five other defendants who face almost 3,000 counts of murder. They could get the death penalty if convicted in a trial that is likely at least a year away. Most of this week's proceedings have focused on abstract pretrial legal issues.
Fried helped in the transfer of 40 detainees overseas during his four years as special envoy, assuming the post shortly after Obama first took office and promised to close the much-maligned prison within a year.
But Republican-led bills have since cut off funding to move detainees to foreign countries, and bringing them to the United States has been impossible since Congress blocked Obama's attempt in 2009 to try Mohammed and others accused of war crimes in a civilian court.
The Obama administration still hopes to close Guantánamo and send its remaining 166 inmates elsewhere, but officials say congressional restrictions have left diplomatic efforts severely hampered.
Despite signing last year's federal defense bill, the president criticized further provisions it included that regulated the detention, interrogation and prosecution of suspected terrorists. He called continued Guantánamo restrictions "unwise" and insisted federal courts can successfully prosecute terrorists.


text:
Price per frame reduces reduces the larger the pack
8" x 3"
These are top quality purse frames with a really nice shiny silver finish. They have large bobble clips that close together securely. Use with our Gutermann Textile Glue to fix fabric to frame for a perfect finish.
We also stock these frames with fixed loops. If you choose the frames with loops you can clip a chain to it to make your own shoulder bag.
Your name *


text:
|Home Lingomigo Fanmigo! Travelmigo Minglepet Mingleprep Minglepoet Rideshare Roommates Free Online Dating Free Indian Matrimonial Mingle Catalogs Recipes Jobs Mobile Games Internet Radio Ringtones Online Games Internet TV Nightclubs Gay Bars Spas Gentlemen's Clubs Tattoo Parlors Golf Courses Counseling Wedding Photographers Appraisers Chiropractors Life Coaches Attorneys Plastic Surgeons ESL Schools Handbags Flash Audio Video Chat Poems Vacation Rentals iMingle
|Searching thousands of poems by title, poet and keyword.
|Find Poems Results: 1 - 9 of about 465 for dreams
|Other Poem/Poetry Searches: friendship hope dreams life love wedding divorce birthday death shakespeare keats milton walt whitman dylan thomas mark twain ogden nash more
(no spam...)
check if you're already listed before adding a new entry
{} link to sites not affiliated to, or endorsed by rockle.com
|© 2005 - 2009 Rockle - Rockle - Feedback


text:
Everyone warned me about the windy weather I'd experience out West here. For the first few days it wasn't bad, but I notice the wind starts to strike up in the afternoon. Morning walks are the best.
It's amazing how big the wide open spaces are, I think of the song..
"I Can see for miles and miles and miles" Fluffy who wrote that one?


text:
As has been the standard operating procedure recently, Hampton Coliseum made the announcement on its Facebook page: The great Bootsy Collins will headline the 2013 Hampton Funk Fest.
The event, set to take place Friday, Feb. 1, at Hampton Coliseum, will also feature Zapp, Rose Royce, Con Funk Shun and Lenny Williams.
Tickets go on sale 10 a.m. Friday, Dec. 14.
What's the famously funky bass man been up to lately? Last year, he released "Tha Funk Capital of the World," an album that included guest shots from Buckethead, Musiq Soulchild, Ice Cube, Bobby Womack and Samuel L. Jackson, among others.
The disc also featured a tribute to the late, great James Brown, who helped to introduce Bootsy to the world by hiring him for his ground-breaking band.
"What's funny about that is, I was a guitar player before I joined Mr. Brown's band, and I wanted to play bass like Jimi Hendrix," he said in an interview posted on his official website. "Eventually, that's exactly what I did, adding effects and freeing up the instrument to go anywhere I imagined it could go, and that's how Bootsy became Bootsy. But Mr. Brown wanted me to just keep it simple and keep on hitting that 'one.' I used to grumble about it, but today I'm thankful."
Read my review of last year's Hampton Funk Festival -- which featured George Clinton and Chuck Brown among others -- here.


text:
Follow RFF News on your cell phone. Text follow rff to 40404 you will receive txt messages with RFF News
0 Members and 1 Guest are viewing this topic.
Ugot & KK if either of you need a break let me know.
Page created in 0.069 seconds with 34 queries.


text:
CAMBRIDGE, Mass. – The nationally-ranked No. 10 MIT men's track & field team opened the 2012 indoor campaign with a tri-meet victory over Bates College and Colby College, scoring 199 team points on Saturday afternoon at the Shobrys and Aronson Track. Bates took second with 116 and Colby was third with 64 points.
Freshman Sheldon Trotman (Gaithersburg, Md.) had an impressive debut in his first intercollegiate competition, earning a pair of wins along with a third-place finish. Trotman was first in the long jump at 20-4.50 feet and the triple jump at 41-7.25 feet. He also placed third in the high jump, clearing 6-feet even. Teammate Matt Falk (Barnegat, N.J.) was the winner in the high jump, with a 6-4.00 foot effort.
Sophomore Brian Gager (Rye, N.Y.) led the Cardinal and Gray to a sweep of the top three at 400 meters. Gager finished with a time of 52.38 seconds. Freshman Nick Diamantoni (Belle Mead, N.J.) was second in 53.03 seconds and sophomore Jacob Jurewicz (Gurnee, Ill.) took third.
Those three were joined by sophomore Tyler Singer-Clark (Falmouth, Mass.) on the 4x400 meter relay that took first place with a time of 3:29.85, nearly three full seconds ahead of runner-up Bates.
Singer-Clark earned second place in the 200 meters (23.13 seconds) and fourth in the 55-meter dash (6.78 seconds).
Other MIT wins on the track came from freshman Chadd Kiggins (Xenia, Ohio) in the 600 meters, sophomore Justin Bullock (Detroit, Mich.) in the mile and the 4x800 relay.
Senior Nathan Peterson (Kansas City, Mo.) and freshman Benjamin Schreck (Sherman Oaks, Calif.) took one-two in the pole vault. Peterson cleared 14-5.25 feet and Schreck made it over the bar at 13-11.25 feet.
The Engineers will travel to Springfield College for an invitational next Saturday.


text:
Denver, CO - Mile High Kickball Open (Co-Ed Division) #MHKO2017 Kickball · Co-Ed Co-Ed Division Circuit Event
- Team Fees
- Early Bird $50.00 per player + $2.95 Processing Fee
- Regular $55.00 per player + $2.95 Processing Fee
- Late $90.00 per player + $2.95 Processing Fee
- Referee Fees
- Free
Mile High Kickball Open
July 29-30, 2017
What Is A Circuit Event?
A Circuit Event is a weekend long Kickball Tournament hosted by Kickball365. Expectations of a Circuit Event include: Thursday Night Bar Crawl (select cities), Friday Meet and Greet, Saturday Coed Tournament, Saturday Night After Party, Sunday Women's Tournament, and Sunday Night After Party. The series of ALL Circuit Events in a calendar year that lead up to Circuit Championship Weekend in November is known as The National Tour.
Lowry Sports Complex. 8200 E. Sports Blvd, Denver, CO 80230. There is NO official transportation from Kickball365 to or from the fields. Public transportation via Uber or Hertz Rental Car is highly recommended.
This tournament is open to ANY and ALL adult kickball teams with no previous qualifications necessary to participate. The Coed Division plays on Saturday and the Women's Division plays on Sunday. Teams who compete in this event have a chance to qualify for Circuit Championship Weekend in November.
Event Rules
All Circuit Events utilize the Official Rulebook of Kickball365.
Prize Pool
Kickball365 will award cash prizes to teams approximately one (1) week after the completion of this Circuit Event. The total amount will be based on the final number of teams participating.
Team Seeding
After registration closes, each team in attendance will be assigned a tournament seed. Each seed will be based on a team�??�?�¢??s averaged ranks (i.e. 1st, 2nd, 3rd) in each of the individual ranking systems �??�?�¢?? ELO, FIFA�???�??�?�®, and RPI �??�?�¢?? to generate their tournament ranking. New Teams without enough data to make such a determination will be seeded at the bottom to complete the seeding. From this initial seeding, each team will be put into a bin with similar seeded teams. The captains in the first bin will draw opponents from the second bin of teams into their pool. Then those second bin captains get to draw the third team in their pool from the next bin and so on until all pools have been finalized by captains. By contrast, the CIRCUIT SCORING SYSTEM (CSS), which awards points to registered Circuit Teams solely on their performance at Circuit Events, will be used to seed teams at the Circuit Championship Weekend.
Player Eligibility
You must be at least 21 years old (as of the posted event date) to participate in this tournament and the social events planned during the weekend. You must be in good health, have adequate health insurance, and be physically prepared to compete in this event.
Player Registration
All Captains, Players, and Non-Playing Coaches are required to register themselves online prior to the posted registration deadlines (at 1159pm EST/859pm PST). We do not accept offline payments of any kind and cannot manually add anyone to your team if they failed to register on time.
New Player Referral Program
If you have any friends, close contacts, co-workers, or anyone else who has never played in a Circuit Event before, they will receive FREE ENTRY to their first event. This program allows for up to two (2) players PER TEAM per tournament. Players must register using the code NEWKICKBALLER2017 at the time of registration. This option is available for one time use only, meaning a female playing in two (2) events in the same weekend can only use the code for one (1) of those events.
Non-Playing Coaches
Each team is allowed, but not required, to have a maximum of two (2) Non-Playing Coaches. These individuals are required to officially register for the Circuit Event at no cost using the discount code NONPLAYINGCOACH2017 before the same player registration deadline. This includes signing the same waiver that all players must sign. As a reminder, a Non-Playing Coach is allowed to engage with the Game Referees ONLY if they are wearing the required Captain�??�?�¢??s Band during the Game.
Required Check-in at HQ
All Captains, Players, and Non-Playing Coaches will be required to check-in at HQ prior to the start of their first game and will need to bring formal identification (ex. valid driver's license or passport). Check-in begins one (1) hour prior to start of the first game. Failure to properly check in may result in a yellow card for the player and/or team captain.
Morning Meetings
Approximately 30 minutes prior to the start of games for the day, all Captains, Non-Playing Coaches, and Referees are required to attend a brief meeting at HQ. Ground rules for the day will be covered and you will have an opportunity to ask questions in an interactive environment.
Captains, Players, and Non-Playing Coaches are to be held accountable for the behavior of their teammates, supporters and guests. If the conduct of any member of such group or groups is deemed by Kickball365 to be unsportsmanlike or disruptive to the competition, then those individuals may be subject to disciplinary actions up to and including ejection from the park.
Schedule/Standings/Watch the Games
- The game day schedule and standings can be found at Scoreboard365.com
- Spectators are encouraged to watch games from the sidelines only. Please do not sit anywhere where you might interfere with any live plays.
- If you cannot attend the event in person you can watch a live stream of the event on WatchKickball.com
- If you are live streaming on Periscope, please link your account to Twitter and put '@kickball365' anywhere in the title. This way every live streamed game can be easily found on our public Twitter Feed.
If you are no longer able to attend the event, please submit an email to firstname.lastname@example.com. If your request is submitted before the last registration deadline (at 1159pm EST/859pm PST), Kickball365 will issue you a 100% site credit that can be used at any future Circuit event. No credits will be granted after the final registration date under any circumstances. Kickball365 does not provide monetary refunds outside of these site credits.


text:
Welcome to the Fun With The Fuzz 5k website! The Fun With the Fuzz 5K is a road race in Bellingham Washington that is open to both runners and walkers. 100% of the proceeds from this race go directly to the Behind the Badge Foundation which is responsible for taking care of the families of fallen officers as well as maintaining the Washington State Law Enforcement Memorial located in Olympia.
This race hopes to provide assistance as well as awareness for this great foundation.
Every year, we at Fun With The Fuzz 5k attempt to give you the best event experience we possibly can. We want to make sure you enjoy everything from start to finish. Here are some of the things that make the Fun With The Fuzz 5K one of the most unique events in Bellingham!
- Cash prize for the overall 1st, 2nd, and 3rd place finishers of the Men's and Women's Divisions!
- The course is open to both runners and walkers
- Fun with the Fuzz reusable grocery bags with your race packet pickup.
- Pre-race packet pickup the day before the race at the Bellingham Police Department
- Chip-timed race - Accustat Sports Timing will be on hand to give you live finishing times
- Professional race photography by Jon Brunk Photography
- T-shirts for the first 1500 registered
- Coffee provided by Starbucks
- Post-race pizza provided by Little Caesars and other refreshments provided by Haggen
- Fun with the Fuzz ribbons for the top finishers in each age group
- Great raffle giveaways from the Diehl Ford, Scotty Browns, Roger Jobs Motors and more!!!
- Plus much more...
Behind the Badge Foundation
The mission of the Behind the Badge Foundation is to honor the sacrifices of those officers who have died or suffered a catastrophic injury in the line of duty by:
- Serving as the trusted resource to families and surviving officers
- Providing immediate and on-going support
- Maintaining the Law Enforcement Memorial as eternal recognition and remembrance for fallen Washington officers.


text: Anschluss (än´shlŏŏs), German term designating the incorporation of Austria into Germany in the 1930s. Anschluss was first advocated by Austrian Social Democrats. The 1919 peace treaty of St. Germain prohibited Anschluss, to prevent a resurgence of a strong Germany. After Hitler's rise to power the Nazis took over the idea. In 1938, Austrian Chancellor Schuschnigg was forced to agree to Hitler's demands for Anschluss, but reneged, calling for a plebiscite. After the Chancellor's forced resignation, the Austrian president refused to name an Austrian Nazi, Seyss-Inquart, to replace him, and the German agent in Vienna telegrammed for German troops. Adolf Hitler occupied Austria on Mar. 11, 1938, and, to popular approval, annexed it as the province of Ostmark. In the Moscow Declaration (1943) the United States, Great Britain, and the Soviet Union annulled the Anschluss, recognizing Austria's right to independence; an independent government was not established until the end of World War II.


text:
Khalil Ahmad Fitri
Article Images
ASGHABAT (Pajhwok): The Lapis Lazuli Transit, Trade & Transport Route agreement was signed today at the 7th Regional Economic Cooperation Conference on Afghanistan (RECCA-VII) summit held in Ashgabat, the capital of Turkmenistan,... read more
KABUL (Pajhwok): Participants of the 7th Regional Economic Cooperation Conference (RECCA-VII) who gathered in the Turkmen capital, Ashgabat, on Tuesday stressed empowering Afghan businesswomen and increasing the bilateral annual trade volume between... read more
ASHGABAT (Pajhwok): The Lapis Lazuli Transit Route agreement is expected to be signed among the five member countries during the 7th Regional Economic Cooperation Conference on Afghanistan (RECCA-VII) conference on Wednesday. The RECCA conference... read more
KABUL (Pajhwok): Three civilians were wounded in a suicide attack on a foreign forces' convoy in the Afshar area of Kabul on Sunday, the Ministry of Interior (MoI) said. A statement from the ministry said the suicide bomber in a car targeted the... read more
KABUL (Pajhwok): In his Eid message, former president Hamid Karzai has said instability and war in Afghanistan was for the interest of others, but only victimized Afghans. Hamid Karzai, in his message, also felicitated his countrymen and Muslims... read more
KUNDUZ CITY/ZARANJ/HERAT CITY (Pajhwok): At least 15 militants have been killed and a dozen wounded during clashes in the Chardara and Aliabad districts of northern Kunduz province, an official said on Saturday. The fighting erupted when... read more
KABUL (Pajhwok): A number of lawmakers on Saturday stressed that the government should specify a date for parliamentary elections. Based on article 83 of the constitution, parliamentary polls should have taken place by now, but the Independent... read more
BRUSSELS (Pajhwok): NATO Secretary-General Anders Fogh Rasmussen on Thursday said the alliance was planning to support Afghanistan with a new mission to train, advise and assist its security forces after 2014. "But we need to be very... read more
BRUSSELS (Pajhwok): NATO defence ministers and counterparts from ISAF partner nations on Thursday discussed with Afghan defence minister Bismillah Khan Mohammadi the progress in Afghanistan mission and post-2014 planning."We are focused on... read more
BRUSSELS (Pajhwok): A two-day NATO defence ministerial meeting got under way in this capital city of Belgium on Wednesday to discuss issues including a key security pact between Kabul and Washington, as well as the landmark presidential elections.A... read more


text:
Shipping to: Sverige
Classic false eyelashes, easy to put on and reusable, with glue included.
Made of synthetic fiber with a natural, light consistency, they add intensity to any look.
Available in 2 varieties:
01 Natural - Classic strip false eyelashes give volume to your eyes and ultra-feminine length to your lashes.
02 Natural - side plumed lashes, to lengthen the shape of your eyes and give an intriguing "cat-eye look".
03 Natural - individual lash extensions in a set of 60 pieces in 3 different lengths.
Extremely versatile, allows your lashes to be filled in however you like with a natural looking result, suitable for any occasion.
Dermatologically and ophthalmologically tested glue.
Application of lashes on strip 01 and 02:
Make up your eyes however you like, using only a light coat of mascara, trying to curl your lashes upwards as much as possible.
Gently detach the false eyelashes from the backing.
Position the false eyelashes on your eyelid to define the application trajectory.
If the lashes are too long for your eyes, trim them as needed.
Spread the glue along the line of the false eyelashes using the stick applicator attached to the cap and wait for about one minute, until the glue has become nearly transparent.
Apply the false lashes close to the roots of the natural lashes, pressing gently, from the outside corner of the eye towards the inside.
If you like, complete your eye make up with eyeliner to hide any visible glue from the attachment.
To remove the false lashes, gently pull from the outer corner.
For proper reuse, remove any glue residues and place the eyelashes in their case.
Application of lash extensions 03:
Make up your eyes however you like, using only a light coat of mascara, trying to curl your lashes upwards as much as possible.
Remove the lash extensions from the backing using tweezers and coat the root with the glue.
Wait a few minutes until the glue becomes nearly transparent.
Still using the tweezers, position the lash extension directly on the natural lash root starting from the outer part of the eyelid and working inwards, depending on the areas that you wish to intensify.
For a natural look, apply the longer extensions to the outer part of the eyelid and the shorter ones on the inner part.
Complete your eye make up with eyeliner to hide any visible glue from the attachment.
To remove the false lashes, gently pull from the outer corner.
For proper reuse, remove any glue residues and place the eyelashes in their case.
The cost varies depending on the delivery service and the destination country of the order. For more details see the "Delivery information and costs" page.
More information
Shipping times may vary depending on the service chosen. In the countries served by Swiss Post (Asendia) only, delivery takes an average of 6-7 working days (up to a maximum of 10) from the order being dispatched. In countries where the Skynet service is also available, the shipping time is 2-4 days after being dispatched by courier. More information
Purchases made on KIKO are secure and guaranteed. All information relating to payments, including personal information and credit card information, is protected through an advanced encrypted data transmission system. Under no circumstances will KIKO file your credit card number, nor will it use or keep the information you provided on its own systems.
You have 14 business days from the date you receive the items in which to exercise your right to return the goods. Please let Customer Service know about any problems relating to the goods received.
To use a promotional code, select "Have you got a promotional code?" on the shopping cart page and insert the characters indicated in the appropriate field. If your code is inserted correctly, the amount and/or content of the Shopping Cart will be recalculated based on the promotion applied.
Volumizing mascara for fuller-looking lashes with a panoramic effect
Veramente super!
Ottime per i principianti!


text:
inedomedia necesitaba un nuevo diseño de botón o icono y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 75 diseños de 22 diseñadores freelance.
Inedo Media Client: Slickedit
We handle all the ads on software/tech sites The Daily WTF/Stack Overflow.
This banner will be for one of our most loyal advertisers, Slick Edit (http://www.slickedit.com/).
**This banner will specifically be a part of a temporary promotion for them as a part of their over-all ad campaign with us so NO NEED for the banner to capture the look and feel of their site/branding. It can be radically different if well done.
For inspiration, check out the differences between Sourcegear's company site and summer campaign (banner can't be uploaded but looks very similar to landing page):
http://www.sourcegear.com/ company site
http://sourcegear.com/fortress/stackoverflow/ landing page (specific to SO though they ran this same banner/landing page combo on several sites).
Software developers, any kind. Specifically the diligent and talented ones reading Stack Overflow or The Daily WTF
banner size is 728x90. If you do animation or flash, be prepared to also submit a good static version.
Here's some copy to play with:
"SlickEdit Cash for Clunky Editors Program!
If the editor you purchased is lacking the speed, power, and flexibility SlickEdit provides…trade it in for CASH towards SlickEdit. Call 1-800-934-EDIT (3348) or email firstname.lastname@example.com, and mention the Cash for Clunky Editors Program to learn about the savings. The program expires on September 30th, 2009, so act quickly."
Overall, we want this banner to have a "hot off the presses"/ news headline feel to it. To receive this discount the reader MUST call or email (preferably call) so this info can be in the banner but not mandatory. This same copy will be prominent on the landing page so you may want to just make it look like an important update and use the "SlickEdit Cash for Clunky Editors Program!" Really, the banner just needs to be compelling enough to create attention/desire to click on it.
the landing page for this campaign will be a modified version of this:
SEE ** note in the "About Us" section.


text:
Rep's local premiere reimagines final night in civil-rights hero's life
"We've got some difficult days ahead. But it doesn't matter with me now. Because I've been to the mountaintop. … And I've looked over. And I've seen the promised land. I may not get there with you. But I want you to know tonight, that we, as a people, will get to the promised land."
At the moment the Rev.
She had yearned to see King speak, but there had been a bomb threat against the civil-rights leader. So Carrie May Golden's mother made her stay away.
The next day, King was assassinated.
More than 40 years later, Golden sat in a London theater, watching "The Mountaintop" — a play about that final night in King's life, written by her daughter, Katori Hall. When the character of a maid named Camae — short for "Carrie May" — took the stage, Golden had a very vocal reaction.
"She freaked out because she had forgotten I named the character after her," Hall recalls with a laugh. "It was in a moment where there was nothing funny happening in the play, so people didn't understand why this lady was (making such a scene)."
"The Mountaintop"
When: Previews begin Saturday. Opens March 8. Performances: 8 p.m. Thursdays-Saturdays; 2 p.m. Sundays (no matinee March 3); plus 2 p.m. some Saturdays and 7 p.m. some Sundays, Tuesdays and Wednesdays. Through March 31.
Where: San Diego Rep's Lyceum Space, 79 Horton Plaza, Gaslamp Quarter
Tickets: $31-$52; $18 students; discounts for seniors, military and groups
Phone: (619) 544-1000
Online: sdrep.org
"The Mountaintop," which is about to have its local premiere at San Diego Repertory Theatre, has that habit of provoking reactions, even among people not so close to the work. The play became a minor sensation after its London debut, winning Britain's Olivier Award as best new play in 2010.
The next year, it moved to Broadway for a splashy U.S. premiere, starring Angela Bassett as Camae and
"The Mountaintop," set in Room 306 of Memphis' Lorraine Motel — the place where King would be shot as he stood on a balcony the next day — centers on imagined conversations between the young maid and the passionate but beleaguered preacher and civil-rights hero.
The play takes some surprising turns, venturing into something like magical realism. But what has raised a few hackles is the fact that it portrays King as very human — insecure, even frightened at times, although not afraid to use some profanity.
King's own daughter Bernice, while praising the piece, said in one interview that "I just don't think Daddy spoke that way."
But it wouldn't be like Hall to worry about such concerns. The playwright, at 31 one of the hottest names among emerging theater artists, has demonstrated a distinctive voice and fearless spirit with such works as "Hurt Valley" and "Hoodoo Love" (staged last year at San Diego's Mo`olelo Performing Arts Company).
She says that while "as a black writer, you are shouldered with this responsibility to bring your race forward," she believes her first duty is to the stories she feels compelled to tell.
"I don't write underneath anyone's gaze," Hall says. "I don't write underneath a white gaze or a black gaze or anyone else's. I just always write what I want to write. And sometimes that gets me in trouble.
"People in the black community — not necessarily artists, they can be politicians or my parents — they're like, 'Why are you writing about black people in that way? Don't you feel as though you could paint us in a good light?'
"And I always say: 'I don't write black and white characters. I write gray characters.' "
Fearless foray
Hall has found a kindred soul in
Smith himself has a reputation for, as he puts it, "taking on the icon," with warts-and-all solo shows about such prominent African-American figures as Huey Newton (for which he won an Obie Award) and Frederick Douglass. So he appreciates the authenticity of Hall's writing voice.
"I think there's an imaginative fearlessness which is remarkable," Smith says of the playwright. "And I think it's really wonderful that this is a young woman who is from Memphis, who was not even born when Dr. King was assassinated, but whose curiosity and sense of history led her to very imaginatively dig into probably the most compelling story in Memphis."
Smith argues that "The Mountaintop" stands only to humanize the revered King, not tear him down.
"He is monolithic — literally, now that we have a monument," says Smith, referring to the King memorial that was dedicated on Washington's National Mall in 2011. "A brilliant man, a man from whom we can learn so much. But a man nonetheless."
Smith notes that the reason King went to Memphis in the first place was to show solidarity with black sanitation workers who were on strike over job discrimination.
"The guys in the strike carried signs," Smith says. "And the signs said, 'I Am a Man.' That's kind of the directive for this piece."
Larry Bates, who is playing King at the Rep (opposite Danielle Moné Truitt as Camae), says playing even an imperfect version of the icon can be intimidating.
"Those are large, large shoes to fill," says Bates. "(But) the great thing about this play is you get to see a different side (of King).
"I'm honored to be playing him, but I'm not trying to live up to the man."
Bates adds that Hall's dialogue feels steeped in the story's place and time.
"I grew up in the South — I grew up in Louisiana," he says. "And I can tell you — and it's the great thing about getting a piece by a good writer — the language sits with you when it's right. (It's) very authentic, very true."
Moving forward
For all that the play has meant to her life and career, Hall says that if she were to write "The Mountaintop" now, "it would be absolutely, completely, totally different."
"I wrote that play at a time in my life, and at a point in America's history, when we were so infused with this optimism and this energy about the possibility of the first black president," she says. "That's totally different from where we are today."
Although she can't say exactly how the work would change, Hall says that "I have a few more gray hairs now, so it would be a little bit more cynical."
Still, Hall isn't spending too much time looking back. As she spoke, she was preparing for a trip to Africa, where she planned to research a new play (part of a trilogy) about the Rwandan genocide.
"It's an epic one," she explains. "Twenty characters. People say, 'You'll never produce it.' And I say, 'Ah ha ha — I'll figure it out.' "


text:
We loves us some Red Green Show and despite it being out of production for years now, it's little gems of genius like this that keep us looking for more. Let's say you drive a beat-to-crap Dodge Ram Van and you don't have power windows — like our hero here, and you're looking for an easy way to upgrade? Why old Red has a brilliant solution to your problem. Next thing you know he'll be building an amphibious Honda Civic DX, oh, wait... [Youtube]
The Red Green Way To Power Windows