text:
I realize that when one undertakes to research both sides of the family, that's a very big undertaking. I've had a lot of help along the way from cousins, my mom, and my sisters. Still there are times I feel as if I'm neglecting one side or the other. Lately, I've been neglecting my dad's side of the family. The Mierzejewskis in Toledo just don't seem to want to be found. But sooner or later, I'm going to tease them out by hook or by crook. Many of the Mierzejewskis that I knew liked a good joke, so it's likely they're still playing games. (And yes, I am half serious by saying that–my dad was a joker and played some serious pranks–some day I should post the story how he answered the phone when my sisters and I were teenagers and boys would call for dates.)
Today is Tombstone Tuesday over at GeneaBloggers. So here are the graves of two possible Mierzejewski relatives. I cannot place them in my tree; however, chances are good I'm related to them. It all goes back to that business of having two grandparents, unrelated but with the same last name before marriage. However, you'll notice the names aren't spelled quite the same way. Like Plenzlers/Plencners, the Mierzejewski name has morphed over time as well.
These are the few graves that I have photos of from Calvary but have not recorded the grave location and cannot look up the grave location. If I ever find the locations, I'll eventually post them.
First grave stone of the day is for Stanley Mizejewski.
I corresponded with Garret Mierzejewski, a Mierzejewski expert. Garret did have an obituary for Stanley. The obituary has no date other than the year, 1962. I've transcribed the obituary below.
Stanley Mizejewski
Stanley Mizejewski, 70, of 9735 Douglas Rd., Temperance, Mich., died Tuesday at Flower Hospital.
Mr. Mizejewski, born in Poland, lived in the Toledo area more than 50 years. He had been a machinist at the Baker Bros. Co. for 35 years, retiring in 1958.
Surviving are his daughters, Mrs. Virginia Berend and Mrs. Marcella McClean; son, Harold S. Brooks, all of San Diego and five grandchildren and two great-grandchildren.
Services will be at 10 a.m. tomorrow in the Urbanski Mortuary, with burial in Calvary Cemetery.
I may be able to find Stanley's grave location if I poke through the Calvary burial logs again. I attempted to last night, but 1962 seemed to be a particularly good year for business at Calvary. I got blurry eyed reading and might have missed it.
Here is the next grave. The stone is very worn, it's difficult to read and I cannot fully transcribe it.
I believe the name on this gravestone may be similar to Konstancia Mierzejewska (the feminine form of the surname). However, it's weather worn and difficult to read. The death year on the stone also is difficult to determine. Is it 1913, 1916, 1918, or 1919? As Garret pointed out to me, the birth year is clear enough to determine that this person was born in the 1850s. Yet as he pointed out, most of Mierzejewskis who came here were born in the 1880s, possibly 1870s. Mierzejewskis emigrated during a later phase of Polish migration to the US: my grandfather was documented as coming to the US as early as 1903 when he was just 18 or 19 and he returned to Poland a few times before settling permanently in Ohio. So this person is a unique surprise and it would be interesting to learn more about him or her.
If anyone has any information, please leave a comment or drop me an email.


text: Interesting. Yesterday, I checked the forum on my iPhone, and saw basically the same them as here, but really tiny. This morning, trying to view the forum, I got only .php files, so something was up. Now, on my iPhone, I see a vBulletin mobile theme - not bad, actually. So if you're going to use this mobile theme, you might want to add the Treeleaf logo, and change the colors to match the full theme. But it's certainly more practical this way.

subset: commoncrawl


text:
On 10/3/08 Glen and his daughter Melissa were on the Dr.Phil Show. Now Sammy Montana and Anarchy Management believe this story will make a GREAT MOVIE
Find them at www.anarchymanagement.com
They are attempting to get the rights optioned.
Visit www.drphil.com/shows/show/1132 to read about the show. Then check out the show's message boards, over 95 pages long.
Stores can order copies of "Unlawful Flight" from Pathway Book Service. Just call 1-800-345-6665.
Glen and his family wish to thank everyone involved in this story for their help; many risked their careers and even prison time by helping them stay together.
To buy now from Amazon or read the
reviews, click here:
Unlawful Flight
This is a true story of how one father had no choice but to take his two children and go on the run.What they find along the way will surprise you, but what they go through to stay together will simply amaze you. From handcuffs to happiness, this story is proof that all obstacles can be overcome with love, faith in God, trust in fellow man and a desire to be together.
When reading this book you will laugh and cry; You will feel fear and joy, love and hate and after reading Unlawful Flight we believe that your faith in God and fellow man will be strengthened!
We do not encourage or condone parental kidnapping or Parental alienation in any way.
Estimates range from 200,000 to 350,000 children are taken each year by a parent or other family member. for the love of our children, It has to stop. Our story is bringing attention to this nightmare and hopefully change will follow. No parent should ever have to kidnap the children again!
Glen is honored to have his book recommended by the group
"Justice not abduction" at www.justicenotabduction.com
Copyright © 2007. Unlawful Flight. All rights reserved.
visitors so far !


text:
Never share your password with anyoneFirst off, never give out your password to share an account. When two people share an account, it links them in the ban system and if one account is banned, the other might be seen as related. But far worse, you're trusting someone with your password who may not keep that trust--they could misuse it themselves, or share it with someone else. Don't ever share your password for any reason.
This goes triple for anyone claiming to be a BYOND admin. Never give your password to anyone who is or claims to be BYOND Staff--the staff will never ask for your password.
We've also had users tell us their previous passwords when they contact us via the support form. Don't do this; it doesn't help you since we can't look up your old (or current) password, and it just compromises your security.
Look out for phishingThe weakest link in any security system is the human element. I can't even count how many users claimed they were "hacked" because someone stole their password via phishing. Phishing isn't hacking--phishing is the user being gullible. Keep a sharp eye out and don't be fooled by these common scams.
If you see a popup in a game ask you for your password, it's not ours. The BYOND pager asks for your password when you login, but Dream Seeker never will. And I shouldn't have to say this, but if the game uses passwords for other reasons, don't use the same password you use for your account--or any account. There's really no guarantee as to how a game will store any password info, and the connection used to send it to the server isn't the same kind of secure link used by our login system.
Phishing works most often by a threat: The phisher will pretend to be legitimate (in this case, they'll pretend to be BYOND Staff) and will say several bad things will happen to your account unless you reenter your login information at the link they give you. In some cases users have gotten fake emails telling them to set their password to a certain value, all while still threatening to do things like delete their hub entries if they don't comply.
Always, always view such emails with extreme suspicion. It's a pretty rare event that we'll ever tell a user to use a specific password, and when we do it's because we already made the change for them in response to an account recovery issue. (In those cases we also request changing it immediately to something we don't know.) We won't tell you to use a certain password you don't already use, because there would be no reason for us to do that. There's no reason for us to ask for your password either. And if you really needed to login again for some reason, you should go directly to the real BYOND site and login there--under no circumstances should you follow the link in the email telling you that if you don't do it bad things will happen.
Phishers also like to pretend there's time pressure, that if you don't respond immediately you'll face terrible consequences. Don't fall for this. We want you to take the time to investigate. If something smells even a little fishy to you, it probably is.
In the event you ever receive an email from BYOND Staff that you weren't expecting, you can always follow up by asking us to confirm it using our customer contact form. There's no harm in doing this. Usually such emails from us are in the form of: "We heard you were violating Terms of Service in such-and-such a way, so please cut it out." If an unsolicited email is asking you to login or wants to do something with your account info, that should raise a few red flags. Save the email and ask us about it. If it does turn out to be bogus, there's a good chance the phisher left some digital fingerprints behind.


text:
- Read the latest issue of the ProbesOnline E-Newsletter.
- Measure cell health and toxicity with high-content imaging and analysis assays
- A powerful tool for measuring cytotoxicity that survives fixation and permeabilization
- Visualize protein expression in cells
- Image late-stage apoptosis before cells "pop"
- Primary antibodies against ATP synthase (Complex V) and other mitochondrial proteins
- A robust and sensitive chloride assay
- Directly labeled primary antibodies for flow cytometry
- proven performers
- buzzworthy
- the view
- on the web
- in the field
HCS DNA Damage Kit
what they are
Two new assays for automated imaging platforms provide multiparametric analysis of important biological events related to cell stress, damage, and death.
how they work
The HCS DNA Damage Kit measures DNA damage using antibody-based detection of phosphorylated H2AX (Ser139) in nuclei. The HCS Mitochondrial Health Kit measures mitotoxicity with the MitoHealth stain, which accumulates in the mitochondria of live cells in an amount proportional to the mitochondrial membrane potential. Both kits include the Image-iT® DEAD Green™ Viability Stain for measuring cytotoxicity; drugs and test compounds that cause serious cell injuries, including plasma membrane permeability, allow entry of the dye.
what they offer
- comprehensive analyses of cell health and toxicity—simultaneous detection of prelethal and lethal aspects of cellular stress and viability
- content-rich results—both assays include reagents compatible with fixation and detergent-based permeabilization, enabling the addition of other antibody detection steps to the assay
- user-friendly protocols—simple and robust methods
|Cat. no.
|HCS Mitochondrial Health Kit, 2-plate size
|1 kit
|HCS DNA Damage Kit, 2-plate size
|1 kit
what it is
The Image-iT® DEAD Green™ Viability Stain not only measures cytotoxicity, but survives fixation and detergent-based permeabilization, making it ideal for use in HCS assays and standard fluorescence microscopy imaging applications.
how it works
Cytotoxicity is a multifaceted process that often leads to compromised plasma membrane integrity. The Image-iT® DEAD Green™ Viability Stain is impermeant to healthy cells, and can enter cells only after plasma membrane integrity has been compromised. In contrast to viability stains like propidium iodide, the Image-iT® DEAD Green™ Viability Stain is compatible with fixation and permeabilization, enabling multiparametric analyses with other biomarkers or cytotoxicity indicators.
what it offers
- content-rich results—survives fixation and detergent permabilization, enabling subsequent multiparametric analyses with antibodies
- simple protocol—add to cells, then incubate
|Cytotoxicity measured using the Image-iT® DEAD Green™ Viability Stain. HeLa cells were treated with DMSO (untreated) or 60 μM valinomycin for 18 hr, stained with Image-iT® DEAD Green™ Viability Stain for 30 min, fixed, and imaged using the Thermo Scientific Cellomics® ArrayScan® VTI Platform.
|Cat. no.
|Image-iT® DEAD Green™ Viability Stain, 1 mM solution in DMSO
|25 μl
what it is
The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay provides a fast, sensitive, nontoxic, and nonradioactive method for the detection of nascent protein synthesis using fluorescence microscopy and high-content imaging.
how it works
Click-iT® AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT® AHA is added to cultured cells, then incorporated into proteins during active protein synthesis. Detection of the incorporated amino acid uses a chemoselective ligation or "click" reaction between an azide and an alkyne, where the azido-modified protein is detected with Alexa Fluor® 488 alkyne.
what it offers
- the first imaging assay for detecting newly synthesized proteins or changes in protein expression
- validated in A549 and U2OS cells with reagents that inhibit protein synthesis, including cycloheximide, anisomycin, and puromycin, in both dose response and min/max format
- content-rich results—compatible with fixation and detergent-based permeabilization, enabling the addition of other antibody detection steps to the assay
|Dose response curves performed in duplicate. U2OS cells were treated in L-methionine–free media and 50 μM Click-iT® AHA for 30 min with anisomycin (17 pM–1 μM). Cells were washed, fixed, permeabilized, and nascent protein synthesis was detected following a click reaction with Alexa Fluor® 488 alkyne. Imaging and analysis was performed using the Thermo Scientific Cellomics® ArrayScan® VTI Platform.
|Cat. no.
|Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay, 2-plate size
|1 kit
what they are
The Click-iT® TUNEL Imaging Assays can detect a higher percentage of apoptotic cells than assays that use other modified nucleotides under identical conditions. The assay is complete within 2 hours and allows multiplexing with surface and intracellular biomarker detection.
how they work
The terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL) assay is based on the incorporation of modified dUTPs by the enzyme terminal deoxynuclotidyl transferase (TdT) at the 3'-OH ends of fragmented DNA, a hallmark as well as the ultimate determinate of apoptosis. Often at this final stage of apoptosis, adherent cells are known to detach or "pop" off. For a reliable and reproducible TUNEL imaging assay, it is vital that the modified nucleotide is an acceptable substrate for TdT, and that the detection method is sensitive without causing any additional cell loss. The Click-iT® TUNEL Imaging Assays use dUTP modified with an alkyne, a small, bio-orthogonal functional group that allows the nucleotide to be more readily incorporated by TdT than other modified nucleotides are. Detection is based on a click reaction, a copper (I) catalyzed reaction between an azide and alkyne. Click chemistry fills the void when methods such as direct labeling or the use of antibodies are not efficient. The small size of the Alexa Fluor® azide (MW <~1000) compared to that of an antibody (MW ~150,000) enables easy penetration of complex samples, with only mild fixation and permeabilization required.
what they offer
- detection of a higher percentage of apoptotic cells under identical assay conditions compared with fluorescein-dUTP or BrdUTP
- results within 2 hr
- accurate, consistent performance with content-rich results
|DNA strand breaks typical of late-stage apoptosis visualized using the Click-iT® TUNEL Imaging Assay. HeLa cells were treated with staurosporine, then fixed and permeabilized. The Click-iT® TUNEL Alexa Fluor® 647 Imaging Assay (Cat. no. C10247) was performed. Activated caspase-3 was detected with a polyclonal rabbit primary antibody for cleaved caspase-3 and labeled with Alexa Fluor® 488 goat anti–rabbit IgG (Cat. no. A11008, green fluorescence). Tubulin was detected with a mouse monoclonal anti-tubulin antibody and labeled with Alexa Fluor® 555 goat anti–mouse IgG (Cat. no. A21422, orange fluorescence). Nuclei were stained with Hoechst 33342 (Cat. no. H1399, blue fluorescence). The coverslips were mounted in ProLong® Gold antifade reagent (Cat. no. P36930) before imaging. The cell on the right not only has a high level of caspase-3 activity and DNA strand breaks, but also shows a loss of structural integrity consistent with a cell undergoing apoptosis.
|Cat. no.
|Click-iT® TUNEL Alexa Fluor® 488 Imaging Assay, for microscopy and HCS
|50–100 assays
|Click-iT® TUNEL Alexa Fluor® 594 Imaging Assay, for microscopy and HCS
|50–100 assays
|Click-iT® TUNEL Alexa Fluor® 647 Imaging Assay, for microscopy and HCS
|50–100 assays
what they are
Invitrogen offers the most complete portfolio of antibodies for mitochondrial research, including antibodies against ATP synthase (Complex V). New this month are antibodies against nicotinamide nucleotide transhydrogenase (NNT) and mitochondrial trifunctional protein (TFP), as well as the ApoTrack™ Cytochrome c WB Cocktail for detecting apoptosis.
how they work
The most important function of mitochondria is production of ATP; deficiencies in this process result in many genetic and neurological diseases. Complex V, also called F 1F 0-ATPase and ATP synthase, is responsible for ATP production in oxidative phosphorylation, and can work in reverse as a proton-pumping ATPase. The enzyme in mammals is composed of 17 subunits, 5 of which make up the easily detached F 1. The remainder are components of two-stalk domains and the proton-pumping F 0 part of the machinery. Invitrogen offers antibodies against subunits of ATP synthase as well as other mitochondrial proteins that work closely with the electron transport chain—including new antibodies against nicotinamide nucleotide transhydrogenase (NNT) and mitochondrial trifunctional protein (TFP). Nicotinamide nucleotide transhydrogenase (NNT) is a mitochondrial inner membrane protein catalyzing the interconversion of NADH and NADPH within the matrix space of the organelle. NNT couples this reaction to proton translocation across the inner membrane. Mitochondrial trifunctional protein (TFP), located in the inner mitochondrial membrane, is part of the mitochondrial fatty acid beta-oxidation pathway. TFP catalyzes three out of the four steps in the beta-oxidation cycle, with a specific affinity for long chain substrates. Inherited deficiency of TFP is a recessive genetic disease that occurs in approximately 1:38,000 newborns in the US. Deficiency of LCHAD (the dehydrogenase part of TFP) has also been found in children of women who develop HELLP syndrome (1:1,000 pregnancies) and AFLP syndrome (1:13,000 pregnancies), both of which are life-threatening obstetric conditions. For mitochondrial researchers studying apoptosis, the ApoTrack™ Cytochrome c WB Cocktail provides a method for detecting the release of cytochrome c and other apoptogenic proteins from the mitochondrial intermembrane space into the cytoplasm.
what they offer
- the best specificity for mitochondrial proteins
- validated reagents with multiple applications and species specificities
- part of an extensive portfolio of antibodies for mitochondrial research
|Staining of HDFn cells. HDFn cells were detected with nicotinamide nucleotide transhydrogenase (NNT) monoclonal antibody (Cat. no. 459170) and labeled with Alexa Fluor® 594 goat anti–mouse IgG1 secondary antibody (Cat. no. A11005).
|Cat. no.
|ApoTrack™ Cytochrome c WB Cocktail
|100 μg
|Nicotinamide Nucleotide Transhydrogenase (NNT) monoclonal antibody (WB and ICC)
|Hu, B, Rt, Ms
|100 μg
|Mitochondrial Trifunctional Protein (MTP) monclonal antibody (WB)
|180 μg
|Complex V d-subunit Monoclonal Antibody
|Hu, Ms, B, Rt, Ce
|100 μg
|Complex V immunocapture monoclonal antibody
|Hu, B, Rt, Ms
|100 μg
|Complex V subunit alpha monoclonal antibody
|Hu, B
|100 μg
|Complex V subunit F6 monoclonal antibody
|Hu, B, Ms
|100 μg
|Complex V Mitoprofile® Immunocapture Kit
|Ms, Rt
|100 μg
|Hu = human; B = bat; Rt = rat; Ms = mouse; Ce = C. elegans.
For a complete list of mitochondrial research antibodies, visit www.invitrogen.com/antibodies.
Premo™ Halide Sensor
what it is
The Premo™ Halide Sensor was developed for functional studies of ligand- and voltage-gated chloride channels. Transient BacMam-mediated expression of a halide-sensitive yellow fluorescent protein (YFP) mutant is efficient in a range of cell types, including human primary cells.
how it works
The combination of BacMam delivery, the bright fluorescence of the Venus variant of Aequorea victoria green fluorescent protein (GFP), and a quenching iodide surrogate ion provides a highly sensitive assay for robust and convenient screening of halide ion channels and transporter modulators in your preferred mammalian cellular model.
what it offers
- chloride channel–specific—measure halide ion flux in voltage- and ligand-gated chloride channels and transporters
- robust—reliably high expression, no-wash format, excellent signal window, and compatibility with most fluorescent HTS readers
- flexible—study chloride flux in your cellular model with efficient and noncytopathic delivery of Premo™ Halide Sensor by BacMam technology
- pharmacologically relevant—known modulators show dose-dependent quenching, and BacMam delivery enables assays in primary cells
Principle of the Premo™ Halide Sensor. Iodide redistribution upon chloride channel activation. Basal fluorescence from Premo™ Halide Sensor is high when chloride channels are low. Upon activation (opening) of chloride channels, the iodide ions enter the cell down its concentration gradient, quenching the fluorescence from the Premo™ Halide Sensor.
|Cat. no.
|Premo™ Halide Sensor for 10 microplates
|1 kit
Learn more about the Premo™ Halide Sensor
what they are
Invitrogen offers new primary antibodies conjugated to Qdot® nanocrystals, Alexa Fluor® dyes, Pacific Blue™ dye, and Pacific Orange™ dye, with validation for flow cytometry.
how they work
Directly conjugated primary antibodies are available in a variety of fluorescent colors to maximize multicolor flow cytometric analysis. These antibodies give researchers the tools to find answers to complex biological questions relating to cancer, immunology, stem cell research, and activation studies. Simultaneous measurement of different specificities reveals more information about individual cells in less time, with less sample.
what they offer
- more options for multicolor analysis
- greater flexibility and precision in designing multicolor experiments
- tools to satisfy the growing need for more information per sample
To browse antibodies by specificity or application, visit www.invitrogen.com/antibodies.
To find antibodies and other reagents for flow cytometry, visit www.invitrogen.com/flowcytometry.
|Ntera2 cells stained with Alexa Fluor® 647 dye–conjugated anti-SSEA4 monoclonal antibody (Cat. no. SSEA421). The negative profile represents unstained cells.
|Regulatory status
|Cat. no.
|CD1a, Mouse anti-human Alexa Fluor® 488
|0.5 ml
|CD2, Mouse anti-human, Qdot® 605 conjugate*
|100 μl
|CD3, Hamster anti-mouse PE-Alexa Fluor® 700
|1.0 ml
|CD8, Mouse anti-human, Qdot® 565 conjugate*
|100 μl
|CD10, Mouse anti-human, Qdot® 605 conjugate*
|100 μl
|CD15, Mouse anti human Pacific Blue™
|0.5 ml
|CD16, Mouse anti-human Pacific Blue™
|0.5 ml
|CD16, Mouse anti-human Pacific Orange™
|0.5 ml
|CD19, Mouse anti-human, Qdot® 655 conjugate*
|100 μl
CD20, Mouse anti-human Pacific Blue™
|0.5 ml
|CD23, Mouse anti-human Alexa Fluor® 488
|0.5 ml
|CD29, Mouse anti-human Alexa Fluor® 488
|0.5 ml
|CD45, Rat anti-mouse Pacific Blue™
|1.0 ml/100 μg
|CD45R(B220), Rat anti-mouse, Qdot® 655 conjugate*
|100 μl
|CD56, Mouse anti-human Alexa Fluor® 488
|0.5 ml
|CD69, Hamster anti-mouse Pacific Blue™
|1.0 ml/100 μg
|CD105, Mouse anti-human Alexa Fluor® 488
|0.5 ml
|HLA-DR, Mouse anti-human Pacific Blue™
|0.5 ml
|HLA-DR, Mouse anti-human, Qdot® 605 conjugate*
|100 μl
|HLA-DR, Mouse anti-human, Qdot® 800 conjugate*
|100 μl
|IFN-Gamma, Mouse anti-human Pacific Blue™
|0.5 ml
|Mouse anti-SSEA4 Alexa Fluor® 647
|0.5 ml
|Mouse anti-SSEA4 Alexa Fluor® 700
|0.5 ml
|Hamster IgG Isotype control PerCP
|0.5 ml/ 50 ug
|Hamster IgG Isotype control PE-Alexa Fluor® 700
|0.5 ml
|* Qdot conjugates are optimally excited by UV lasers or 405 nm light, but may be excited by other lasers as well.
what it is
Zenon® labeling technology provides a rapid, versatile, and reliable method for producing fluorescently labeled antibodies using a variety of fluorochromes, even with small amounts of unpurified starting material such as hybridoma culture supernatant. Antibodies labeled using Zenon® labeling technology are suitable for use in many applications where a directly labeled antibody can be used. Zenon® labeling technology provides a versatile, easy-to-use system for labeling human, mouse IgG1, IgG2a, and IgG2b antibodies, and rabbit IgG antibodies.
how it works
Zenon® fragments are specifically designed to target and bind to the Fc portion of the primary antibody only, giving a rapid, noncovalent method of quickly labeling small quantities of primary antibody. Zenon® labeling technology is simple and efficient; the entire labeling procedure takes only 10 min. Once the Zenon® antibody complex has formed, it can be stored until needed; just before use, nonspecific IgG is added to block any unbound Zenon® fragments, making column purification unnecessary. The Zenon® complex can then be applied directly to the sample.
what it offers
- 10-min labeling time
- 1–20 µg primary antibody per labeling
- compatible with BSA and other stabilizing proteins
- no purification step
- flexible antibody labeling
Now through March 31, 2009, visit www.invitrogen.com/zenon and take a short survey to receive 25% off your next purchase of Zenon® products.
Bhardwaj, A. et al. (2007) Blood 109: 2293–2302.
Can natural products help outwit cancer cell defenses? The potential anticancer benefits of resveratrol—a naturally occurring polyphenol abundant in red grapes—have been demonstrated in a wide variety of tumor cells. However, its effect in multiple myeloma (MM) cells, which can be particularly resistant to existing chemotherapies, has not been investigated. Chemoresistance in MM cells has been shown to involve numerous mechanisms, including overexpression of antiapoptotic substances and activation of cell survival pathways. In the present study, the authors examined the effect of resveratrol treatment on the proliferation and antiapoptotic potential of drug-sensitive and drug-resistant MM cells. They observed significant suppression of MM cell proliferation in response to resveratrol treatment, as well as potentiation of the apoptotic effect of two drugs widely used to treat MM, as revealed through use of the Molecular Probes® LIVE/DEAD® assay. Resveratrol treatment also caused MM cells to accumulate in the sub-G1 cell-cycle phase, accompanied by an increase in apoptosis-related substances and measurable downregulation of antiapoptotic gene products. Significantly, their results showed that resveratrol inhibited both inducible and constitutive NF-κB activity, and suppressed several genes whose products are known to contribute to oncogenesis. Their results offer the first evidence that resveratrol is effective in overcoming chemotherapy-induced resistance in MM cells, offering promise for its potential use to enhance the efficacy of standard chemotherapeutic agents.
View bibliography reference
|Find products for the integrin pathway
Empower your research today with Invitrogen's comprehensive portfolio of products and services for investigating integrin signaling. You'll find everything from high-quality reagents for basic research and assay development to validated biochemical and cell-based assays, as well as world-class profiling and screening services. View our portfolio of reagents for integrin signaling at www.invitrogen.com/integrin.
Join Invitrogen at the upcoming High-Content Analysis meeting hosted by the Cambridge Healthtech Institute at The Fairmont Hotel in San Francisco, California, USA, January 6–9, 2009. Along with leaders from across the industry, we will be presenting our latest tools and assays for high-content analysis platforms. Stop by and see us at booth #14.
Molecular Probes® The Handbook
©2008 Molecular Probes, Inc. Visit our website for a complete listing of legal notices and trademark attributions. Feel free to distribute ProbesOnline to friends and colleagues, but please keep this copyright statement intact.
cellular imaging │high-content screening │ HCS Mitotic Index Kit
what it is
The HCS Mitotic Index Kit is a powerful image-based assay for the identification of compounds that affect mitotic progression.
how it works
The HCS Mitotic Index Kit includes a primary antibody against phosphorylated histone H3 (Ser10) as a sensitive index of mitosis, and a secondary antibody conjugated to the green-fluorescent Alexa Fluor® 488 dye. Blue-fluorescent DAPI and near-infrared–fluorescent HCS NuclearMask™ Deep Red stain, both included in the kit, provide two choices for DNA profiling and cell demarcation for image analysis.
what it offers
- comprehensive analyses of cell health and toxicity—simultaneous detection of prelethal and lethal aspects of cellular stress and viability
- content-rich results—both assays include reagents compatible with fixation and detergent-based permeabilization, enabling the addition of other antibody-based parameters to the assay
- simple, user-friendly protocol
Dose response for nocodazole in A549 cells using the HCS Mitotic Index Kit. A549 cells were treated with nocodazole at final concentrations between 0 to 1,000 nM and incubated for 24 hr. Imaging and analysis were performed using a 10X objective and the Compartmental Analysis BioApplication with the Thermo Scientific Cellomics® ArrayScan® VTI platform.
|Cat. no.
|HCS Mitotic Index Kit
|1 kit


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September 13th
CHAPTER TEN -- the last chapter -- NOW POSTEDThe waiting is finally over ... The days leading up to and following Bella's 19th birthday – the year she never turned 19. And yes, this is a sequel to "August 13th."
ATTENTION READERS: DO NOT STEAL MY STORIES. Someone has stolen some of my stories from this website -- including this one! -- and posted them as their own on fanfiction.net. It is plaigarism, it is stealing and it is illegal. Read, enjoy -- but don't steal. Post-Eclipse This is a sequel to "August 13th" -- you don't have to have read that story to understand this one, though I highly recommend it! ;) To do so, check it out here: http://www.twilightarchives.com/viewstory.php?sid=986
1. Chapter 1
Rating 4.5/5 Word Count 1419 Review this Chapter
* * *
* * *
I finished taping up another cardboard box and stood back, surveying the room. It was so different-looking, with my belongings no longer scattered about. Instead, they were packed up in boxes and bags – labeled for the "apartment" Charlie believed Edward and I would be sharing near campus. In the absence of my things, I noticed the fine layer of dust covering the furniture and floor. Scattered bits of dirt and tiny scraps of paper and a lone spider web in one corner. Somehow, when I wasn't paying attention, life had happened in this room. The dust and grime was evidence of it all. And now I was leaving it behind; just a memory locked away in a room.
I sighed and used my legs to push the box aside with the others. Edward had offered to come help me pack – besides his agility at the heavy lifting, he had promised he would make a quick job out of the whole ordeal. But part of me wanted these last few moments alone with the life I was about to leave. It was like saying goodbye. I needed to do it alone.
We'd gotten back to Forks about a week ago, after taking four days following the wedding for a sort of honeymoon. It was nothing fancy and Alice highly disapproved, but it was exactly the right thing for me. Edward had planned it: four days in a quiet cabin on Lake Cushman on the other side of the Olympic National Forest. I'd never heard of it, but Edward said it would be perfect. Of course, he was right. The tiny little cabin nestled amongst the trees on the far side of the lake – away from the tourists and campgrounds – was the perfect escape for us. We spent all day together in bed – tempting fate, as Edward liked to call it – though after our first time, he had been less hesitant to try again a few more times.
"It seems your impatient human hormones have won," he'd said. "And they seem to have brought out my own impatient hormones."
"That would be the man in you?"
And when we finally roused ourselves from bed in the evening, we spent twilight under the stars – floating together in the water and inevitably encouraging our impatient hormones. Edward, at least, still managed some restraint.
When we got back to Forks, we had just over a week left to pack and head off to "college." We weren't going to college, not now; but that was our cover. It's what Charlie and our friends believed. I tried to make time to finish my goodbyes properly. It was more difficult than I'd imagined. It's not easy saying goodbye to people who think you're only saying, "So long for now." It is impossible to convey the finality of the farewell on someone who doesn't understand that this is it. It did not stop me from trying. I made time for Angela and even Jessica and Mike. I spent more time with Charlie, even watching a couple Mariner's games with him on TV. I didn't really understand what was going on, but after the first four innings I finally asked what the numbers on the top of the screen meant – and Charlie spent the better part of the evening explaining the finer details of baseball to me. My head was dizzy when we were finished and I didn't remember much, but my interest had made Charlie happy. I couldn't forget the enthusiasm with which he explained the difference between a foul and a walk and the necessity of extra innings.
And his happiness in those moments alone made it worth it. These were mere seconds compared to the life that awaited me with Edward. In a matter of days, this life would be over; just a memory. I was determined to make the most of it.
I closed my eyes and reopened them carefully, trying to focus once again on the task at hand – to finish packing up my room; my life. I pulled out another cardboard box, taped it together and began filling it with the scattered contents of my desk drawer. I was contemplating the necessity of keeping most of the items I'd found – a protractor, extra loose-leaf binder paper, an eraser, a pencil sharpener and a few scattered paper clips – when a soft knock came at the door.
"Yeah?" I called, turning – expecting to see Charlie. "Oh," I said, surprised when a different face appeared at the door.
I had not planned on seeing Jake again.
He grinned sheepishly as he stepped into the room – his large frame making the empty space seem crowded again; smaller. "Charlie said you were packing," he said quietly.
"Um, yeah," I replied, tucking my hair behind my ears. Needlessly, I wiped my dusty hands across the thighs of my jeans. "We're leaving for … college … in a couple days."
"Yeah," he said. "I know. College." He sighed – a loud, deep and overly dramatic sound.
I rolled my eyes. "Are you just here to give me more crap?" I asked. "Or was there something else you wanted?"
He shrugged. "I just wanted to come by … see you one last time."
I eyed him warily for a long moment, taking in his expression and the way he stood slightly hunched over – his hands tucked in the pockets of his jeans. He was fully clothed, so he must have driven here like a normal person. His eyes darted away from my gaze, flickering around the room – taking in the boxes and the dust and the blankets folded neatly at the foot of my empty bed. "You're a masochist, aren't you?" I finally asked.
He turned and looked at me, his eyes wide with surprise. "What?"
"You just love torturing yourself, don't you? I heard about how you ran away – after Edward sent you that invitation. He wasn't supposed to do that, you know. And then you popping by on the wedding day … and now this. You're addicted, you know that? You just can't be happy. You like being miserable."
I expected him to respond with some rude quip, but he didn't say anything right away. He looked down at his hands, his fingers twisting, before meeting my gaze once again. "Yes," he said, the words just a whisper on his lips. "I think you're right. I think I'd rather be miserable than accept the truth."
"Oh, Jake …"
I was debating whether or not to go to him when the door opened again with a whoosh of cool air and then hard, icy arms were circling my waist. "Bella," Edward said, leaning down to touch his lips to mine.
I closed my eyes, falling into the kiss – despite its brevity. "How did you get in?"
Edward laughed, amused by my question. "Charlie let me in."
"Oh," I said, surprised. Usually, Edward bypassed Charlie and just climbed through the window. Reflexively, my eyes flickered to the glass panes.
Edward laughed again, sensing the reason for my surprise. "We're married now, Bella. I don't have to sneak through the window anymore."
"He snuck through your window?"
Edward and I turned to see Jake staring at us, a light blush burning through the dark pallor of his cheeks. His hands were clenched in fists at his sides. At the same time, I felt Edward's arms tighten around my waist. I don't think he was aware of the action. I sighed; this had to end. I squeezed Edward's hands reassuringly, then took three steps forward.
"Goodbye, Jake," I said. "I will miss you. I hope you can forgive me someday." Carefully – mechanically – I wrapped my arms around his waist, then reached up on tiptoes and chastely kissed his cheek. Then – just as carefully as I had approached him – I stepped back into the circle of Edward's arms; my husband's arms. I'd said my farewell and I'd made my choice. I was just reinforcing that decision.
Jake, sensing the finality of the moment, sighed again heavily. He reached out one of his very long arms towards my head – as if to muss my hair – but then seemed to think better of it and retracted his arm, wiggling his fingers instead. "Bye, Bells."
* * *
- Author
- Kayla Ariev
- Rating
1 2 3 4 5
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- 15 Dec 07
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text:
Stage3D for iPad and iPhone
December 07th 2011
Crocus Modeller - a sweet tool for developers
August 05th 2011
Flash Player 11 and AIR 3 beta's out on Labs
July 13th 2011
Flash Builder 4.5 and Flex 4.5 Update Available Today
June 20th 2011
Adobe AIR 2.7 available
June 15th 2011
Flash Player 10.3 for desktop and Android available
May 13th 2011
Abobe announcing CS5.5 update
April 11th 2011
Molehill preview released on Labs
February 27th 2011
Flash Player 10.2 almost ready for the masses
February 06th 2011
Adobe MAX Sneak Peeks
October 27th 2010
July 04th 2012 | peterelst | Published in Community
A lot has happened over the last couple of years changing the landscape for Flash, particularly when it comes to mobile devices. There was a point not so long ago where there was a clear resurgence and major platforms prominently advertised Flash Player support - those days are no longer.
March 12th 2012 | Jens C Brynildsen | Published in Tutorials
The camera is what you view the 3D world through. Just as a real camera, the virtual 3D camera applies perspective to your models by adjusting properties such as zoom, focus and position. In this tutorial you'll learn how to use the 3 cameras available in Away3D.
March 12th 2012 | Jens C Brynildsen | Published in Tutorials
When you look out through a window in your house, you only see what is visible from your point of view, not what is occluded by the walls. The viewable area is decided by the size and shape of the window, so you'll see a "cropped" view of what's outside. This is exactly how the View works in Away3D as well. It is the "window" that we view our 3D world through.
March 12th 2012 | Jens C Brynildsen | Published in Tutorials
This tutorial is the first in a series covering Away3D version 4, a powerful real-time 3D engine for Flash or Flex. This tutorial explains a very simple Away3D class line by line so that even novices to Actionscript programming will be able to understand what is going on in the rest of the tutorials.
September 15th 2011 | Jens C Brynildsen | Published in Community
The Elevator Pitch was a huge success last year and this year again it was a whirlwind tour of great ideas and projects that are strange, fun, stupid, clever and really everything in between. This day also held the surprise many talked about in the corridors - this was indeed the last Flash on The Beach
Flash Player 11 and AIR 3 beta's out on Labs


text: 7 Smaller Circles In A Larger Small 320×320 Medium 640×640 Large 1024×1024 Download TIFF Original 3000×3000 | (305.5 KB) Download EPS Vector 353.8 KB Description A large circle containing 7 smaller congruent circles. The small circles are externally tangent to each other and internally tangent to the larger circle. Keywords circle, circles, circumscribed, externally, inscribed, internally, tangency, tangent Galleries Circles Source: Florida Center for Instructional Technology Clipart ETC (Tampa: University of South Florida, 2007)


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Houston Congresswoman Sheila Jackson Lee has the conservative blogosphere in a tizzy again today. The target of right-wing wrath is the liberal Democrat's contention that Congress should look "carefully at how we reform entitlements that are not handouts but they are earned."
Video of Jackson Lee's speech quickly ricocheted around the Internet. Comments on a Fox News blog post were almost universally negative: The Houston lawmaker was labeled "a disgrace" and "increasingly appalling."
In their eagerness to embarrass Jackson Lee, some conservative blogs simply "copied and pasted" Fox News' transcription of the speech, including a misspelling of the word "eligibility." (Fox spelled it "eligability.")
Here's what Jackson Lee said on the House floor:
"I believe it should be pronounced here today, that the reform of the entitlements based upon shipshod [sic], reckless, deliberations — or should I say actions — are a non-starter," she said.
Among the non-starters listed by Jackson Lee:
- Raising the eligibility age for Medicare
- Carelessly considered Medicaid changes
- Changes in Social Security, which, she said, "is solvent."
"So, I would say move quickly to pass the middle class tax cuts that would be for all Americans — 100 percent [of Americans] up to $250,000," she said. "And let's think about moving into 2013, how we make this economy better by looking carefully at how we reform entitlements that are not handouts but they are earned."
Critics immediately assumed that Jackson Lee was talking about "welfare" programs, Food Stamps and other social safety net programs that are frequently deemed "entitlements" in the bureaucratic language of Washington.
Two of the three "entitlements" mentioned in her speech are not means-tested programs but government guarantees to elderly Americans who pay into the trust funds for those programs through taxation of on-the-job earnings. The third, Medicaid, is a government health-care program that largely benefits the poor.
Watch the embedded video above and decide for yourself.
Slideshow: Browse Photos of Sheila Jackson Lee


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Decided that drybags could be a cheap but durable DIY floatation idea. Purchased a couple of 80 lt bags for £16 the pair & filled with electronic air-filled packing (about to be thrown out ) These multi-celled thingies are almost impossible to burst ( I think they housed computer equipment )
They equal in volume the YAK's I have in the red canoe so I'm confident of their worth should they be tested.
The drybag material is very resilient & TBH they wont let you ( or themselves) down in the event of accidental puncture un-like the YAK's.
Filled with polystyrene or balls as has been suggested could solve a quick but necessary fix. Anything is better than nothing but regards looks ? - - - - - not too shabby !
Will lash the bags in before adventuring


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The official EAST GRINSTEAD RFC app
FUN Starts Tomorrow...
The Gearon Pavilion Saint Hill Road East Grinstead West Sussex RH19 4JU View with Google Maps
This website is part of the Pitchero Rugby Union network. Create your own free Rugby Union club website with Pitchero.
Digital advertising by Minute Media
© Pitch Hero Ltd 2008-2019
Pitch Hero Ltd Registered in ENGLAND | WF3 1DR | Company Number - 636 1033


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|Macaroni Salad with loads of crunchy veggies, in a mayo and sour cream seasoned dressing, and tossed with black and green olives.
Macaroni and Olive SaladI love pasta salads, even if they aren't all that photogenic. Though this one is loaded with all kinds of veggies for crunch, the central feature of this pasta salad is olives - both black and green.
I've already included pimentos as an add-in with this pasta salad, but use a stuffed olive if you like, or any of your own favorite pitted green olives. I've actually even used olive salad before in place of green olives, when I've had some leftover from making Muffuletta Sandwiches, and it is an excellent, tangy addition, but if you prefer black olives over green, it's still a great pasta salad with black alone.
It always seems kinda silly to share recipes for pasta salad, because what is it but a pasta combined with vegetables and then dressed in either a vinaigrette or mayonnaise based dressing? They are all so much alike, that is true... but yet, each are somewhat different, so I guess it's good to share the different combinations, right?
Recipe: Macaroni and Olive Salad©From the Kitchen of Deep South Dish
Prep time: 10 min |Inactive time: 24 hours | Yield: About 4 to 6 servings
- 2 cups of dry elbow macaroni
- Extra virgin olive oil
- 1/2 cup of mayonnaise
- 1/2 cup of sour cream
- 1/4 cup of milk
- 2 tablespoons pickle relish
- 1/2 teaspoon of dry mustard
- 1/4 teaspoon Creole or Cajun seasoning (like Slap Ya Mama), optional
- 1 teaspoon of chopped fresh basil
- Juice of half a lemon, optional
- Kosher salt and freshly cracked black pepper, to taste
- 1/2 cup of Vidalia or other sweet onion, chopped
- 1/4 cup of chopped celery
- 1/2 cup of sliced carrots
- 1/2 cup of sliced radishes
- 1 (2 ounce) jar of chopped pimentos, drained
- 1 (15 ounce) can of pitted black olives, drained & halved or sliced
- 1/2 cup of sliced or halved pitted green olives
- 1 cup of cubed Mozzarella, optional
Bring a large pot of water to a boil and generously salt. Add the macaroni and cook according to package directions; drain, rinse and drizzle lightly with olive oil. Transfer to a large bowl. In a small bowl blend together the mayonnaise, sour cream, milk, pickle relish, dry mustard, Cajun seasoning, basil, lemon juice and salt and pepper; set aside. Add the onion, celery, carrots, radishes and pimentos to the pasta and mix in. Stir in the mayonnaise blend and mix well; add olives and toss lightly, just to mix. Taste and adjust salt and pepper as needed, add cheese if using, toss, cover and refrigerate for 24 hours before serving, if possible, stirring occasionally.
Cook's Notes: As always with pasta salads, veggie measurements and add-ins are estimates; use more or less to your liking. I used sweet pickle relish, Coleman's spicy dry mustard and fresh basil from the garden. Feel free to substitute dried basil instead but keep in mind you'll want to reduce the amount of dried. Green olives have a distinctly different taste from black olives, so if you're not a fan of the green ones, just eliminate them and add in extra black olives. Feel free to also substitute your favorite short cut pasta for the elbow macaroni - rotini would work nicely here.
Source: http://deepsouthdish.com
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©Deep South Dish
☛ Are you on Facebook? If you haven't already, come and join the party! We have a lot of fun & there's always room for one more at the table.
Check These Recipes Out Too Y'all!
Bacon Ranch Pasta Salad
Shrimp and Macaroni Salad
Tri-Color Rotini Italian Pasta Salad


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Most recognize that public relations is important, but is it more than just writing press releases?
As defined by the Public Relations Society of America (PRSA), "it is a communications discipline that engages and informs key audiences, builds important relationships and brings vital information back into an organization for analysis and action."
In fact, PR involves much more than getting press coverage, and because social media is tailor-made for communicating and engaging with your public, it's increasingly part of the PR toolbox.
You're probably already using some forms of social media to help meet your PR goals, build your business and grow your thought leadership online.
Crossing over
But now there's evidence that even the most traditional of PR roles – the targeting of traditional media – is being impacted by the social media space. A national survey has found that an overwhelming majority of reporters and editors now depend on social media sources when researching their stories:
- 89% said they turn to blogs for story research
- 65% to social media sites such as Facebook and LinkedIn
- 52% to microblogging services such as Twitter
Research and references with a difference
What does this mean? For example, a journalist now will often look at recent blog posts to get a feel for an organization's positioning before writing a story. If you are in a consulting business, they may review your LinkedIn profile to get a feel for your qualifications, or research Twitter to see what others say about you.
This means that even when you're trying to get attention from traditional print and broadcast media, social media plays a critical role in your visibility and outreach.
For those of us running our own businesses and interested in obtaining new clients and customers, social media is no longer optional. A single home base –which, depending on your business, could be a blog, a LinkedIn profile, or maybe even a presence on a review site like Yelp – is necessary at a minimum to spread the word.
Are you at a loss as to where to begin?
If you'd like some more background on social media and the role it now plays in promoting your business, check out these resources:
- Luckie's 30-Day Social Media Makeover
- Groundswell: Winning in a World Transformed by Social Technologies, by Charlene Li and Josh Bernoff (book)
- The New Rules of Marketing and PR: by David Meerman Scott (book)
- Social Media Trends for 2010, by Jeremiah Owyang (excellent slides for intermediate and advanced social media "learners")
- The Modern PR series (on Solo PR Pro)
What role do you think social media will play in public relations (agree or disagree)? What resources would you add to the list?
More from:
- Women Grow Business guest blogger Michelle Tennant on how the Internet changed publicity
- Regular contributor Lori Saitz on the myth of control and how social media creates transparent customer service
- Get cozy with this video recap of integrating traditional marketing with social media from the 2009 GrowSmartBiz conference
Image: Josh Kenzer, Creative Commons
Kellye Crane is an accomplished, award-winning communicator with more than 20 years of experience in strategic public relations, social media, and marketing communications. An in-demand speaker, Kellye addresses the intersection of social media and PR on her Solo PR Pro blog, which serves as a resource for those working as independent consultants — and those who'd like to be. She's frequently listed as one of the top 100 PR pros to follow on Twitter.Google+


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Wynonna Judd's husband has leg amputated
Country star WYNONNA JUDD's rocker husband has had his left leg amputated after sustaining serious injuries in a weekend motorcycle accident.
Michael Moser, aka Cactus, was travelling on a highway near Hill City, South Dakota on Saturday afternoon (18Aug12) when he allegedly veered across the centre of the road and collided with an oncoming car.
He was airlifted to hospital via helicopter, but doctors found his leg had been "severed" in the crash and they were forced to amputate it above the knee.
Moser, the drummer in Judd's band, also had to undergo surgery on his hand, according to TMZ.com.
A representative for the singer says, "(Judd and Moser) appreciate all of the quick medical and police responders as well as the hospital staff and well-wishes from family, friends and fans."
Judd, who wed Moser in June (12), cancelled a planned concert in South Dakota on Saturday night as a result of the crash, while she has also postponed a series of gigs in Canada this week (begs20Aug12) to remain by her husband's side.


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The Woodrow Wilson Teaching Fellowships
Choose a State
Application Process
Applications will only be accepted online and through the Woodrow Wilson National Fellowship Foundation. Due to continued interest, the application remains open.
The Teaching Fellowship application has been aligned with graduate admission requirements and will be reviewed at all participating universities. You do not need to apply separately or submit any supporting documentation to a partner university.
There is no application fee to apply to the Woodrow Wilson Teaching Fellowship program. For technical issues with the online application system, email Tech Support.
- • Getting Started
- • Create An Account
- • Supplemental Items
- • Required Tests
- • Interview
- • Financial Aid
- • Decision
Getting Started
Check your eligibility.
The Fellowship is open to college seniors, graduates, and career changers who:
- • have majored in and/or have 30 or more college-level credits in a STEM field (science, technology, engineering, or math);
- • demonstrate a commitment to the program and its goals;
- • have U.S. citizenship or permanent residency;
- • have attained, or expect to attain by June 30, 2019, a bachelor's degree from an accredited U.S. college or university or its international equivalent (Note: Undergraduate degrees earned outside the U.S. are accepted if an approved credential evaluator declares the degree equivalent to an earned U.S. bachelor's degree.);
- • a cumulative undergraduate grade point average (GPA) of 3.0 or better on a 4.0 scale is preferred (Note: Candidates who can demonstrate excellence through other avenues will also be considered. All applications are considered in their entirety and selection is based on merit.)
Questions about your eligibility? Email Anna Gallos, Program Assistant for Fellow Selection ([email protected]).
Select your institution(s).
Please refer to the detailed information on our university partners to select the institution(s) to which you will apply.
Review financial aid opportunities.
Each of the participating institutions provides financial aid opportunities for graduate students. Should you be interested in applying for need-based financial aid from the federal government, you will need to complete the Free Application for Federal Student Aid (FAFSA) which can be found at www.fafsa.ed.gov.
The Federal government offers loan forgiveness of up to $17,500 in Federal loans for math and science teachers who 1) meet the highly-qualified teacher status, 2) teach for five consecutive years, and 3) teach for these five years in a low-income school. As a Teaching Fellow, you should easily meet the criteria of being a highly-qualified teacher in a low-income school. For more information, please visit the Federal Loan Forgiveness Website. You may also qualify for a Teach Grant.
Create an account
Begin the application process by creating an applicant account here. Creating an account the Woodrow Wilson application Web site will enable you to save your unfinished application so that you can return later to add or change entries and view the status of supplemental items (i.e. transcripts, resume, and recommendations). It is recommended that you save the Web page as a bookmark.
There is no fee to create an account. You will need to provide basic contact information and choose a username and password. Any information collected is used only for purposes of the Woodrow Wilson Teaching Fellowship (see the Foundation's privacy policy).
After creating an account, you will be taken to your personal profile page where you will be able to return at any time to edit your personal information, change your password, register for events, start and/or complete your application, and submit and/or verify receipt of supplemental items.
To be considered for the Woodrow Wilson Teaching Fellowship, you must complete all items on the application, but you may save the work you have done on parts of the application during any online session, and return to complete other parts during subsequent visits..
To complete an unfinished application simply return to this page and log in with your email and password.
Always remember to click on "save and continue" before leaving a page, or on "save application" before logging out in order to save your work.
Submit supplemental items
In addition to your online application, you must upload a resumé and mail official transcripts for each institution attended.
The resumé—perhaps the most important component of your application—provides you with the opportunity to showcase your excellence.
- • Please be sure to include all colleges/universities attended, along with enrollment dates, as well as information and employment dates for all professional experience.
- • If you won awards or fellowships or excelled in sports or other extracurricular activities, please be sure to include this information on your resume.
- • Any volunteer experience, particularly with adolescents or in an underserved community, is also worth detailing.
The resumé should be a .PDF and the title of the file should include your first and last name but not include spaces (i.e. SmithJohnResume.pdf).
In order to upload your resumé, you should first "save the application" before returning to the "My Account" link at the top of the page.
Select "Submit Supplemental Items." Click "Browse" to select your resumé file then click "Upload" to attach the resumé to your application.
TIP: There is no need to limit your resume to one page for your Woodrow Wilson Fellowship application. Remember: this is an important opportunity to showcase your excellence.
Please request letters of recommendation from two (2) recommenders. Recommenders should be advised they will receive an email notification directly from the Woodrow Wilson Foundation.
Letters of recommendation must come from individuals who are not members of your family. In general, recommenders should comment on your character, your commitment to education and service, and your capacity to work in high-need urban and/or rural contexts.
• If you are still enrolled in a degree program or are a very recent graduate, please include, as one of your recommendations, a letter from at least one faculty member in your major content area who can comment on your academic ability.
• If you have been employed over the past several years and are not able to request a faculty recommendation, please include, as one of your recommendations, a letter from a professional colleague who can comment on your skills/knowledge in your specialty field.
Immediately upon saving your application, please return to your account page and scroll to the bottom. Click on "Submit Supplemental Items."
In the Recommendation Request Section, click on "Start" next to each listed letter of recommendation to initiate the recommendation request process.
After entering your recommender's name and email address, you will have the opportunity to include a personal note in the notification email. It is strongly advised that you provide a "submit by" deadline in this area for your recommenders.
Once you have started the process, your recommenders will receive an email requesting they submit a letter of recommendation on your behalf. They have the option to accept or decline this request. Recommenders who accept the request will be taken to an online form where they will enter some information before uploading and submitting their recommendation letter.
If a recommender declines your request to provide a recommendation, you will be notified by email. If you receive a "decline" notification, you will need to return to your account page and "Start" a new recommendation process with a different recommender.
You also have the ability in this same area of your account page to change a recommender by clicking on "Cancel" and then following the procedures to "Start" a new recommendation process with a different recommender, or to "Resend" a request to one of your recommenders, if necessary.
Note: You will be able to track receipt of your letters of recommendation in the "supplemental items" section of your application. It is your responsibility to follow up with your recommenders to ensure that they have submitted their online recommendation.
Official Transcripts
Copies of official transcripts from all undergraduate and graduate institutions you have attended—this includes any institution that awarded credit for coursework—must be provided with your supplemental items. The transcripts must be provided in an unopened envelope from your institutions(s) and mailed directly to:
The Woodrow Wilson National Fellowship Foundation
Teaching Fellowships
5 Vaughn Drive
Suite 300
Princeton, NJ 08540
Telephone: (609) 452-7007, ext. 141
If you'd like to submit a transcript from a foreign college/university, follow these steps to have your foreign transcript evaluated:
- • Have your official transcript(s) translated (if necessary) into English.
- • Send your transcript(s), course descriptions, and the English translation to a credential evaluation service that is a member of the National Association of Credential Evaluation Services. A fee ranging from $165-$295 is required. Be sure to request a "course-by-course/detail" evaluation. * Foreign transcripts which are already in English still will require a course-by-course/detail evaluation.
- • Have an official copy of the transcript and translation sent to:
- The Woodrow Wilson National Fellowship Foundation
Teaching Fellowships
5 Vaughn Drive
Suite 300
Princeton, NJ 08540
- The Woodrow Wilson National Fellowship Foundation
Required Tests
Candidates must pass the state-required basic skills teaching licensure examination or demonstrate an exemption. We strongly encourage applicants to submit passing licensure exam scores or proof of exemption before the established program application deadlines. To learn more about this requirement, please click here.
Interview (Semi-finalists only)
Interviews for semi-finalists will be conducted at various sites in Georgia and Pennsylvania during October, December, and February, following the application deadlines in October, November and January. Applicants invited to participate in an interview will be asked to select the date and location that best works for them. Detailed information about the interview process will be provided to semi-finalists upon invitation to an interview.
Await a Decision
The Woodrow Wilson Foundation expects to award up to 12 Fellowships at each of the participating institutions. In addition, the Foundation will select a number of alternates who may be offered the Fellowship if those originally selected choose to decline for any reason.
For technical issues with the online application system, email Tech Support.


text:
I was browsing some christian forums when I found this piece. I didn't see any atheists actually reply to it though. (The site allows members of other faiths - or lack of) Well the guy who posted it really wanted to have atheists read it and respond (or, even better- convert). So I thought I'd post it here to gather the intended audience's thoughts/reactions/answers.
Having once been an atheist, I understand the mindset pretty well. Empirical evidence is the best thing to use in dialogue with an atheist or an agnostic. Educated atheists and agnostics will, for the most part, be utterly unresponsive to the gospel so long as intellectual barriers to faith remain. So in this thread I'd like to brainstorm some of the most effective arguments to use when dialoging with educated unbelievers. I'll start with a few and you can all add to them.
1. Fine tuning - the universe is highly finely tuned for the existence of intelligent life. The initial conditions of the big bang, which are not physically necessary but are simply given, are so utterly precise that to alter them by a tiny fraction of a percent would make life and even stars impossible. The fact that the universe just so happened to be finely tuned to allow for life, when it could have in theory been otherwise, points to an intelligent mind behind natural design.
2. The beginning - at a finite point in time, 13.7 billion years ago, the universe began to exist. The universe came to be, out of nothing, exactly as Genesis 1:1 declares. Something must be eternal, otherwise you run into the problem of an infinite regress. Either matter/energy is eternal, or something else is eternal. Matter/energy is not eternal, because it began to exist at the moment of the big bang. Therefore something else is eternal. Working with the evidence of fine tuning in the universe, we can infer that the eternal cause behind the universe must be intelligent, powerful, and intentional. Intelligence is needed to explain the ordered design of the universe, and power and intentionality are required to explain the creation of the universe. An eternal cause that is intelligent, powerful, and intentional is, by definition, God.
3. Human uniqueness - Human beings, though genetically very similar to other organisms, are vastly different from the rest of the animal kingdom. Unlike other animals, human beings posses a reflective and introspective self-awareness. Even if we allow for a purely Darwinian explanation for the development of life, the vast complexity of our unique human consciousness cannot be adequately explained. The bible, however, declares that we are made in the image of God and, hence, posses some of his attributes, albeit in drastically minimized form. If we accept this biblical premise as true, we would expect to find the human cognition utterly unique in the animal kingdom, which is exactly what we find. Even though we share 98% of our genes with chimpanzees, our cognition is dramatically more complex and is in fact indicative of transcendence. Add to all of this the omnipresence of spiritual belief in human societies throughout all of recorded history, and you have good empirical evidence for the uniqueness of the human mind. Given all of this, the biblical explanation for the novelty of the human consciousness has the best explanatory power and ought to be accepted as true.
4. Existence of Aesthetics - Humans are also unique in our recognition of and perception of beauty. When we look at the earth and the larger universe we are struck by a sense of wonder and an appreciation of beauty. What is beauty? Why is it that nature, as perceived through our senses, is pleasing to us? Certainly there is no Darwinian reason for the existence of aesthetics, for what differential reproductive benefit can such a thing bestow? The universe, it seems, is objectively beautiful, a fact that is hard to explain in a purely naturalistic and non-theistic framework. However the bible makes the claim that the universe declares the glory of God. Given this premise we would expect that our perception of nature would evoke feelings of wonder and awe, a sense of beauty and majesty, to point us toward the creator and a recognition of some of his attributes. Once again the biblical worldview explains the phenomena better than a naturalistic framework.
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I wonder who wrote this. It reminds me of a vid I watched recently.
Richard Dawkins vs John Lennox.
"Has science buried god". link:
The universe was created from nothing. Impossible. Nothingness is impossible because there would have to be something there to say (show) (prove) that there was nothing there.
The very fact that there is "somethingness" argues against the idea there was ever "nothingness." Particles pop in and out of existence all the time. Why not a universe?
I found the original source by using a unique phrase from the quoted posting. The original is here.
I read half a dozen replies there, and my head started to hurt. Maybe I'll make an account.
I enjoyed your post there. Way too much psychobabble for me to even start with.
The problem with this site is that this "discussion" is on a part of the site that is marked Christians only. If you post to this as an atheist or any other non-christian tag you will have your post deleted and you will receive a warning from a moderator telling warning you that your account will be deleted if you continue to post into Christian only areas (or something similar, Ive deleted mine so I can't give the exact words ).
1. The universe is not, "finely tuned for the existence of intelligent life" - the universe simply IS, and intelligent life, as well as some not-so-intelligent life has simply adapted to the conditions under which it found itself. For example, there are billions of planets that couldn't possibly support life - could we call those, "finely tuned"? Early Earth, under which life arose, had a largely methane atmosphere, which would have been lethal to all lifeforms today. It was the deaths and decomposition of billions of billions of tiny microscopic organisms, over billions of years, each tiny decomposing body releasing minute amounts of oxygen in the process, that changed Earth's atmosphere from methane to the oxygen/hydrogen/nitrogen atmosphere that we know today. No one can claim "fine tuning" for the 99% of all life that went extinct as the atmosphere was modified, only 1% was able to evolve and adapt to an oxygen-rich environment, and we are descended from that small percentage. Fine tuning had nothing to do with it.
2. Time, for this universe, began at the instant of the universe's springing into existence. Did other universes exist before the one in which we find ourselves? We don't know, and can never know. Evidence indicates that our universe will expand forever, or at least, indefinitely - did another universe behave differently, contracting after time into a "Big Crunch," from which our own universe may have sprung because of some imbalance of forces within the singularity to which the earlier universe may have contracted, resulting in the explosion that gave birth to our own? Again, we don't know, because any information as to what preceded our own universe would have been lost, as time and information would have ended with that contraction. The point is, we can say, "I don't know, I may never know, and I can live with that." The theist, by his very definition, MUST say, "God did it!" without any more evidence than supposition.
3. Human uniqueness - most animals spend their entire lives in their comfort zones. That animal which would ultimately become Homo Sapiens, for whatever reasons, ventured out of his, encountering new and different environments from those his country cousins, the gorillas, chimpanzees and orangutans would, in their secluded forest habitats, ever know. Consequently, our ancestors evolved to meet each new challenge - those that didn't died. We became what we are as a result of adapting to new and changing environments, not because we were constructed in the image of some supernatural entity.
4. Existence of Aesthetics - "Humans are also unique in our recognition of and perception of beauty." - this is hardly worth responding to. The call of a humpback whale can resonate for miles. Can any of us say that the humpback is not describing to other humpbacks what an incredibly beautiful world it is in which he lives? Can we ever know what other animals think? We can't say we humans are unique in that regard, unless we KNOW we are, and we don't. Can anyone honestly tell me that my friend here is not admiring the beauty of the intricate veins he observes in that leaf?
1. fine tuning- has he tried living on a different planet or universe for that matter to show that this is fine tuned? Is it not enough to know we are here and that is it?
2.It is philosophical suicide to come from the universe having a beginning to reach the conclusion that a god made it so.
3.Humans are so unique. In all of the animal kingdom, we are the least adapted to our environment. we are the most cruel and greedy. How about that for unique?
4. i think a bird would see its environment to be beautiful but since I've had no conversation with one i can't tell. This is just a case where man wants to think he is special.
In furthering of your arguments:
1. The fact that in an obscure corner of a rather undistinguished galaxy life exists hardly argues for an entire universe finely tuned for mankind.
2. I don't know why theists go straight for the notion that some person made the cosmos rather than it simply happened for reasons unknown,
3. Every species is special (unique) in some way. That's what being a species is all about. If all critters were alike, there'd be no such thing as a species. "Special" and "species" obviously come from a common root word.
4. Someone who thinks animals have no aesthetic sense has never owned a cat. To see a cat stretched out in the sun on a sunny morning reveals an appreciation for the beauty of a dawning day which escapes many human beings.


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* USA after 15 years of long wait was able to win The Miss Universe contest this year 2012
* 5.5 feet, 20 year's girl was titled the most beautiful girl in the Universe.
Yes! All this was just so close to impossible…but Ms.Olivia changes history back to back.
She is one big example for - "Anything is possible".
I have been reading & watching here videos off lately & have learnt a lot from this beauty queen. Few quick things that has touched & inspired me to a major extend about her:
Beauty: "You got to be more than just a pretty face. It's about how good you feel in your own skin. Smile always because that always radiates and brings in smile on others face too. A beauty is not to show-off but to inspire others."
Grateful: "Treat each day as a gift. Make the most out of it. Be grateful to your loved one. Hug your parents and express your feelings, gratitude to people who mean a lot to you."
Confidence: "My boyfriend cheated on me with my best friend, it hurts but then I didn't let it shatter me. I learnt my lessons, moved on. Didn't let that take over me…focused on my goals and believed truly on myself and on god, as he had some very interesting plans for me".
Being yourself: "All what matters is how you feel about yourself. Don't be judgemental, just have an open mind…keep getting inspired by the people around you and keep learning. Better yourself each day."
Communication: "There is no great feeling than being able to express yourself truly in best words. Choose your words carefully. Words have the power to inspire, hurt, create, collapse, destroy… so I always watch what I say."
Passion: "People call me crazy because I dance & keep singing every time anywhere…but I can't stop. Music is so much in me…the passion in me defines me…keeps me always happy & going for new challenges."
Passion: "People call me crazy because I dance & keep singing every time anywhere…but I can't stop. Music is so much in me…the passion in me defines me…keeps me always happy & going for new challenges."
Her values, beliefs, passion, beauty, words has created history today.
So what are your creating for yourself?
Whom are you inspiring?
Are you yourself?


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I believe universities have an obligation to help our students become aware of the need for sustainable systems.
Who wouldn't want to convert 127 million tons of trash into $1.5 billion of energy and reduce greenhouse gas emissions by 146 million tons (CO2e) per year? That is the annual potential for U.S. landfills if they all install anaerobic digesters, according to a recent paper by Professor of Civil and Environmental Engineering Thomas DiStefano.
In anaerobic digesters, microbes that don't require oxygen - anaerobes - break down organic matter into methane and hydrogen, which can be captured and used to generate electricity. The technology isn't new - several countries in Western Europe already process their trash this way. Those countries, however, require homeowners to separate easily biodegraded materials, such as kitchen scraps and yard waste, from other garbage.
DiStefano has worked with a landfill in Lycoming, Pa., to evaluate the feasibility of the technology for American rubbish, where everything is usually tossed together. Large-scale tests were promising enough that the landfill has contracted with a Belgian company for a full-scale assessment of the economics.
Anaerobic digestion happens naturally in landfills, but not as efficiently as in the digesters. "Diverting biodegradable trash to an engineered vessel has the advantage that the biodegradation happens much more quickly than it does in the landfill," DiStefano says. "Landfills biodegrade trash and produce methane also, but sometimes the degradation takes years, whereas in vessels it takes weeks."
Confining the degradation to enclosed vessels also allows all of the methane to be captured. Even in well-designed and operated landfills, approximately 15 percent to 25 percent of the methane escapes to the atmosphere. Methane is a potent greenhouse gas, so capturing it reduces greenhouse gas emissions in two ways - it isn't released directly from the landfill and it displaces fossil fuels that are typically employed to generate electricity.
DiStefano brings his passion for economic, environmental and social sustainability to the classroom. "I believe universities have an obligation to help our students become aware of the need for sustainable systems," he says. Whether he is teaching future engineers, lawyers, CEOs, lawmakers or others, "they are our leaders of the future. That sounds cliché, but it's true, and they are going to be faced with these problems much more than I will be."
As the Robert L. Rooke Professor of Engineering, DiStefano is also developing a new degree program in environmental engineering that will enable students to study and practice sustainable engineering principles.
Posted Sept. 13, 2010


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Hi foresters!
Just wanted to let you know that I'll be soon starting a portuguese language and culture workshop.
We'll be learning useful, interesting, funny portuguese words & expressions and also a little bit about Portugal's history and culture.
I haven't set a date yet because I was waiting to see how many people were interested and work from there to set a date available for everyone. Despite this, I'm thinking of starting sometime on the next week, possibly on Saturday or Sunday afternoon (not this weekend but the next).
If you're interested just send me an email to [url]email@example.com[/url] and let me know when you're available and even if you have some specific aspect that you would like to learn/discuss in these workshops.
Até já!
It's a Portuguese workshop!
2 posts • Page 1 of 1
It's a Portuguese workshop!
only when the clock stops does time come to life
Re: It's a Portuguese workshop!
So, the date for the first class is set: Sunday, April 3rd - 4pm @ the Forest.
See you there
See you there
only when the clock stops does time come to life
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