text:
WASHINGTON — President Donald Trump is considering more than a dozen candidates to succeed ousted FBI Director James Comey, choosing from a group that includes several lawmakers, attorneys and law enforcement officials.
Trump said Saturday that "we can make a fast decision" on a new FBI director, possibly by late next week, before he leaves on his first foreign trip since taking office.
"Even that is possible," he told reporters when asked whether he could announce his nominee by Friday, when he is scheduled to leave for the Mideast and Europe.
"I think the process is going to go quickly. Almost all of them are very well known," Trump said Saturday aboard the plane that took him to Lynchburg, Virginia, where he gave the commencement address at Liberty University. "They've been vetted over their lifetime essentially, but very well known, highly respected, really talented people. And that's what we want for the FBI."
The Trump administration is looking to fill the job, which requires Senate confirmation, after the president abruptly fired Director James Comey on Tuesday.
Eight candidates had interviews Saturday: Texas Sen. John Cornyn; acting FBI Director Andrew McCabe; attorney Alice Fisher; New York Court of Appeals Judge Michael Garcia; Adam Lee, head of the bureau's office in Richmond, Virginia; federal judge Henry E. Hudson of Virginia; former homeland security adviser Frances Townsend; and former Michigan Rep. Mike Rogers.
The overall list includes South Carolina Rep. Trey Gowdy and former New York City Police Commissioner Ray Kelly, according to two White House officials briefed on the matter who spoke on condition of anonymity to discuss internal planning.
The candidates under consideration as a permanent replacement for Comey:
Cornyn is the No. 2 Senate Republican and a former Texas attorney general and state Supreme Court justice. He has been a member of the Senate GOP leadership team for a decade and serves on the Senate Judiciary Committee. In the aftermath of Comey's dismissal, Cornyn said Trump was "within his authority" to fire him and said it would not affect the investigation of possible Russian ties to Trump's presidential campaign.
The South Carolina Republican is best known for leading the congressional inquiry into the deadly attacks on a U.S. facility in Benghazi, Libya, a panel that oversaw a lengthy grilling of Hillary Clinton in 2015. A former federal prosecutor and state attorney, Gowdy was elected to Congress in the 2010 tea party wave and has focused on law enforcement issues. He originally endorsed Florida Sen. Marco Rubio for president before backing Trump in May 2016.
Rogers is the former chairman of the House Intelligence Committee. He served Michigan in Congress for more than a decade before stepping down in 2015. Rogers worked for the FBI as a special agent based in Chicago in the 1990s and briefly advised Trump's transition team on national security issues. His name was floated as a possible replacement for then-FBI Director Robert Mueller in 2013, and he received support from an association of FBI agents before President Barack Obama chose Comey.
Kelly was commissioner of the New York City Police Department for more than a decade, serving two mayors. In the aftermath of the 9/11 attacks, he created the first counterterrorism bureau of any municipal police department and oversaw a drastic reduction in crime. But Kelly also came under fire for his use of aggressive police tactics, including a program that spied on Muslims and a dramatic spike in the use of stop-and-frisk, which disproportionately affected nonwhite New Yorkers.
Luttig, the general counsel for Boeing Corp., is viewed as a conservative legal powerhouse from his tenure as a judge on the 4th Circuit U.S. Court of Appeals and his time as a Justice Department lawyer. He was considered for two U.S. Supreme Court vacancies that went to Chief Justice John Roberts and Justice Samuel Alito. Luttig clashed with the George W. Bush White House on a prominent terror case, rebuking the administration for its actions in the case involving "enemy combatant" Jose Padilla.
A deputy attorney general under President George W. Bush, Thompson served as the department's No. 2 from 2001 to 2003. Among his most high-profile actions was allowing Syrian-born Canadian citizen Maher Arar to be deported to Syria, where he was tortured, after being falsely named as a terrorist. Thompson also served as U.S. attorney for the Northern District of Georgia and held several high-level positions at PepsiCo.
Abbate is a senior official at the FBI, currently responsible for the bureau's criminal and cyber branch. He previously led FBI field offices in Washington, one of the agency's largest, and in Detroit. He's been deeply involved for years in FBI efforts to fight terrorism, serving in supervisory roles in Iraq and Afghanistan and later overseeing FBI international terrorism investigations as a section chief. He's been with the FBI for more than 20 years, and is one of the FBI officials who interviewed this week for the role of interim director.
Currently a partner at the law firm Latham & Watkins specializing in white-collar criminal and internal investigations, Fisher formerly served as assistant attorney general for the Criminal Division of the Justice Department. Fisher faced resistance from Democrats during her confirmation over her alleged participation in discussions about policies at the detention facility in Guantanamo Bay, Cuba. She also served as deputy special counsel to the Senate special committee that investigated President Bill Clinton's Whitewater scandal. If selected, she would be the bureau's first female director.
A Duke-educated lawyer, McCabe was named last year as the FBI's deputy director, the No. 2 position in the bureau, overseeing significant investigations and operations. Since joining the FBI more than 20 years ago, he's held multiple leadership positions, including overseeing the FBI's national security branch and its Washington field office. McCabe became acting director after Comey was fired, but has shown a repeated willingness to break from White House explanations of the ouster and its characterizations of the Russia investigation.
A former New York prosecutor, Garcia has served as an associate judge on the New York Court of Appeals — the state's highest court — since early 2016. He served as the U.S. attorney in Manhattan from 2005 to 2008, and previously held high-level positions in the Commerce Department, the Justice Department and the Department of Homeland Security.
A former U.S. attorney and Colorado attorney general, Suthers was elected mayor of Colorado Springs in 2015. He is widely respected among state law enforcement and many Colorado Democrats. Suthers was inspired to become a prosecutor after he spent part of an internship in the Colorado Springs district attorney's office watching the trial of a gang of soldiers convicted of killing various citizens, including actor Kelsey Grammer's sister, during a crime spree in the 1970s.
Lee, a longtime agent, is special agent in charge of the FBI's Richmond office. He worked in a variety of positions within the bureau. Before Comey tapped him to lead the Richmond office in 2014, he was section chief of the Public Corruption and Civil Rights Section, investigating some of the highest profile cases against government officials and civil rights violations in recent years. He also led the FBI's global Foreign Corrupt Practices Act and Antitrust Programs.
Hudson is a federal judge in Richmond who earned praise from conservatives when he struck down the centerpiece of the Obama administration's health care law in 2010. He is a George W. Bush appointee who earned the nickname "Hang 'Em High Henry" for his tough-on-crime stand as a federal prosecutor and on the bench. He became a hero to animal rights activists when he sentenced NFL star Michael Vick to nearly two years in prison in 2007 for running a dogfighting ring.
Townsend was homeland security and counterterrorism adviser to President George W. Bush after a series of high-profile Justice Department jobs.
Among other roles, Townsend is a national security analyst for CBS News. She worked as a federal prosecutor in New York under then-U.S. Attorney Rudy Giuliani, focusing on white-collar and organized crime. At the Justice Department, she worked in a variety of jobs including leading the Office of Intelligence Policy and Review, which helped oversee intelligence-gathering activities related to the nation's top secret surveillance court.


text:
Profoto Bag S Plus
Grab the handy, high quality Bag S Plus, pack it with the lights you need and head off on location. The tailor-made carrying Bag S Plus is designed for the photographer who from time to time takes the gear out to a client's location. Complete with an adjustable shoulder strap, it's also an excellent choice for safe and protected storage of your lights at your studio.
The Bag S Plus is part of our popular D2 Duo Kits but can also be bought separately. It stores up to three Profoto monolights.
- Stores up to three Profoto flashes such as B1X, D1 or D2.
- Stores up to two Profoto B1X, D1 or D2 with optional glass dome.
- Made with high-quality nylon fabrics.
- Adjustable inner compartments.
- Sealed with a zipper.
- Handles for easy carrying.
- Comes with an adjustable shoulder strap.

subset: commoncrawl


text:
Psalm 119
1Happy are those whose lives are faultless,
who live according to the law of the LORD.
2Happy are those who follow his commands,
who obey him with all their heart.
3They never do wrong;
they walk in the LORD's ways.
4LORD, you have given us your laws
and told us to obey them faithfully.
5How I hope that I shall be faithful
in keeping your instructions!
6If I pay attention to all your commands,
then I will not be put to shame.
7As I learn your righteous judgements,
I will praise you with a pure heart.
8I will obey your laws;
never abandon me!
9How can young people keep their lives pure?
By obeying your commands.
10With all my heart I try to serve you;
keep me from disobeying your commandments.
11I keep your law in my heart,
so that I will not sin against you.
12I praise you, O LORD;
teach me your ways.
13I will repeat aloud
all the laws you have given.
14I delight in following your commands
more than in having great wealth.
15I study your instructions;
I examine your teachings.
16I take pleasure in your laws;
your commands I will not forget.
17Be good to me, your servant,
so that I may live and obey your teachings.
18Open my eyes, so that I may see
the wonderful truths in your law.
19I am here on earth for just a little while;
do not hide your commands from me.
20My heart aches with longing;
I want to know your judgements at all times.
21You reprimand the proud;
cursed are those who disobey your commands.
22Free me from their insults and scorn,
because I have kept your laws.
23The rulers meet and plot against me,
but I will study your teachings.
24Your instructions give me pleasure;
they are my advisers.
25I lie defeated in the dust;
revive me, as you have promised.
26I confessed all I have done, and you answered me;
27Help me to understand your laws,
and I will meditate on your wonderful teachings.
28I am overcome by sorrow;
strengthen me, as you have promised.
29Keep me from going the wrong way,
and in your goodness teach me your law.
30I have chosen to be obedient;
I have paid attention to your judgements.
31I have followed your instructions, LORD;
don't let me be put to shame.
32I will eagerly obey your commands,
because you will give me more understanding.
33Teach me, LORD, the meaning of your laws,
and I will obey them at all times.
34Explain your law to me, and I will obey it;
I will keep it with all my heart.
35Keep me obedient to your commandments,
because in them I find happiness.
36Give me the desire to obey your laws
rather than to get rich.
37Keep me from paying attention to what is worthless;
be good to me, as you have promised.
38Keep your promise to me, your servant —
the promise you make to those who obey you.
39Save me from the insults I fear;
how wonderful are your judgements!
40I want to obey your commands;
give me new life, for you are righteous.
41Show me how much you love me, LORD,
and save me according to your promise.
42Then I can answer those who insult me
because I trust in your word.
43Enable me to speak the truth at all times,
because my hope is in your judgements.
44I will always obey your law,
for ever and ever.
45I will live in perfect freedom,
because I try to obey your teachings.
46I will announce your commands to kings
and I will not be ashamed.
47I find pleasure in obeying your commands,
because I love them.
48I respect and love your commandments;
I will meditate on your instructions.
49Remember your promise to me, your servant;
it has given me hope.
50Even in my suffering I was comforted
because your promise gave me life.
51The proud are always scornful of me,
but I have not departed from your law.
52I remember your judgements of long ago,
and they bring me comfort, O LORD.
53When I see the wicked breaking your law,
I am filled with anger.
54During my brief earthly life
I compose songs about your commands.
55In the night I remember you, LORD,
and I think about your law.
56I find my happiness
in obeying your commands.
57You are all I want, O LORD;
I promise to obey your laws.
58I ask you with all my heart
to have mercy on me, as you have promised!
59I have considered my conduct,
and I promise to follow your instructions.
60Without delay I hurry
to obey your commands.
61The wicked have laid a trap for me,
but I do not forget your law.
62In the middle of the night I wake up
to praise you for your righteous judgements.
63I am a friend of all who serve you,
of all who obey your laws.
64LORD, the earth is full of your constant love;
teach me your commandments.
65You have kept your promise, LORD,
and you are good to me, your servant.
66Give me wisdom and knowledge,
because I trust in your commands.
67Before you punished me, I used to go wrong,
but now I obey your word.
68How good you are — how kind!
Teach me your commands.
69The proud have told lies about me,
but with all my heart I obey your instructions.
70They have no understanding,
but I find pleasure in your law.
71My punishment was good for me,
because it made me learn your commands.
72The law that you gave means more to me
than all the money in the world.
73You created me, and you keep me safe;
give me understanding, so that I may learn your laws.
74Those who honour you will be glad when they see me,
because I trust in your promise.
75I know that your judgements are righteous, LORD,
and that you punished me because you are faithful.
76Let your constant love comfort me,
as you have promised me, your servant.
77Have mercy on me, and I will live
because I take pleasure in your law.
78May the proud be ashamed for falsely accusing me;
as for me, I will meditate on your instructions.
79May those who honour you come to me —
all those who know your commands.
80May I perfectly obey your commandments
and be spared the shame of defeat.
81I am worn out, LORD, waiting for you to save me;
I place my trust in your word.
82My eyes are tired from watching for what you promised,
while I ask, "When will you help me?"
83I am as useless as a discarded wineskin;
yet I have not forgotten your commands.
84How much longer must I wait?
When will you punish those who persecute me?
85The proud, who do not obey your law,
have dug pits to trap me.
86Your commandments are all trustworthy;
people persecute me with lies — help me!
87They have almost succeeded in killing me,
but I have not neglected your commands.
88Because of your constant love be good to me,
so that I may obey your laws.
89Your word, O LORD, will last for ever;
it is eternal in heaven.
90Your faithfulness endures through all the ages;
you have set the earth in place, and it remains.
91All things remain to this day because of your command,
because they are all your servants.
92If your law had not been the source of my joy,
I would have died from my sufferings.
93I will never neglect your instructions,
because by them you have kept me alive.
94I am yours — save me!
I have tried to obey your commands.
95The wicked are waiting to kill me,
but I will meditate on your laws.
96I have learnt that everything has limits;
but your commandment is perfect.
97How I love your law!
I think about it all day long.
98Your commandment is with me all the time
and makes me wiser than my enemies.
99I understand more than all my teachers,
because I meditate on your instructions.
100I have greater wisdom than those who are old,
because I obey your commands.
101I have avoided all evil conduct,
because I want to obey your word.
102I have not neglected your instructions,
for you yourself are my teacher.
103How sweet is the taste of your instructions —
sweeter even than honey!
104I gain wisdom from your laws,
and so I hate all bad conduct.
105Your word is a lamp to guide me
and a light for my path.
106I will keep my solemn promise
to obey your just instructions.
107My sufferings, LORD, are terrible indeed;
keep me alive, as you have promised.
108Accept my prayer of thanks, O LORD,
and teach me your commands.
109I am always ready to risk my life;
I have not forgotten your law.
110The wicked lay a trap for me,
but I have not disobeyed your commands.
111Your commandments are my eternal possession;
they are the joy of my heart.
112I have decided to obey your laws
until the day I die.
113I hate those who are not completely loyal to you,
but I love your law.
114You are my defender and protector;
I put my hope in your promise.
115Go away from me, you sinful people.
I will obey the commands of my God.
116Give me strength, as you promised, and I shall live;
don't let me be disappointed in my hope!
117Hold me, and I will be safe,
and I will always pay attention to your commands.
118You reject everyone who disobeys your laws;
their deceitful schemes are useless.
119You treat all the wicked like rubbish,
and so I love your instructions.
120Because of you I am afraid;
I am filled with fear because of your judgements.
121I have done what is right and good;
don't abandon me to my enemies!
122Promise that you will help your servant;
don't let the arrogant oppress me!
123My eyes are tired from watching for your saving help,
for the deliverance you promised.
124Treat me according to your constant love,
125I am your servant; give me understanding,
so that I may know your teachings.
126LORD, it is time for you to act,
because people are disobeying your law.
127I love your commands more than gold,
more than the finest gold.
128And so I follow all your instructions;
I hate all wrong ways.
129Your teachings are wonderful;
I obey them with all my heart.
130The explanation of your teachings gives light
and brings wisdom to the ignorant.
131In my desire for your commands
I pant with open mouth.
132Turn to me and have mercy on me
as you do on all those who love you.
133As you have promised, keep me from falling;
don't let me be overcome by evil.
134Save me from those who oppress me,
so that I may obey your commands.
135Bless me with your presence
and teach me your laws.
136My tears pour down like a river,
because people do not obey your law.
137You are righteous, LORD,
and your laws are just.
138The rules that you have given
are completely fair and right.
139My anger burns in me like a fire,
because my enemies disregard your commands.
140How certain your promise is!
How I love it!
141I am unimportant and despised,
but I do not neglect your teachings.
142Your righteousness will last for ever,
and your law is always true.
143I am filled with trouble and anxiety,
but your commandments bring me joy.
144Your instructions are always just;
give me understanding, and I shall live.
145With all my heart I call to you;
answer me, LORD, and I will obey your commands!
146I call to you;
save me, and I will keep your laws.
147Before sunrise I call to you for help;
I place my hope in your promise.
148All night long I lie awake,
to meditate on your instructions.
149Because your love is constant, hear me, O LORD;
show your mercy, and preserve my life!
150My cruel persecutors are coming closer,
people who never keep your law.
151But you are near to me, LORD,
and all your commands are permanent.
152Long ago I learnt about your instructions;
you made them to last for ever.
153Look at my suffering, and save me,
because I have not neglected your law.
154Defend my cause, and set me free;
save me, as you have promised.
155The wicked will not be saved,
for they do not obey your laws.
156But your compassion, LORD, is great;
show your mercy and save me!
157I have many enemies and oppressors,
but I do not fail to obey your laws.
158When I look at those traitors, I am filled with disgust,
because they do not keep your commands.
159See how I love your instructions, LORD.
Your love never changes, so save me!
160The heart of your law is truth,
and all your righteous judgements are eternal.
161Powerful people attack me unjustly,
but I respect your law.
162How happy I am because of your promises —
as happy as someone who finds rich treasure.
163I hate and detest all lies,
164Seven times each day I thank you
for your righteous judgements.
165Those who love your law have perfect security,
and there is nothing that can make them fall.
166I wait for you to save me, LORD,
and I do what you command.
167I obey your teachings;
I love them with all my heart.
168I obey your commands and your instructions;
you see everything I do.
169Let my cry for help reach you, LORD!
Give me understanding, as you have promised.
170Listen to my prayer,
and save me according to your promise!
171I will always praise you,
because you teach me your laws.
172I will sing about your law,
because your commands are just.
173Always be ready to help me,
because I follow your commands.
174How I long for your saving help, O LORD!
I find happiness in your law.
175Give me life, so that I may praise you;
may your instructions help me.
176I wander about like a lost sheep;
so come and look for me, your servant,
because I have not neglected your laws.


text:
Saturday, June 23, 2007
Monday, June 18, 2007
Mt. St. Helens
Sunday, June 17, 2007
Hoh Rainforest
I got in a run of a little over 20 miles on the Hoh River Trail, out and back. Also visited Crescent Lake (great clam chowder) and Olympic hot springs, which were heavenly. I'd always wanted to soak in backcountry hot springs and finally had the chance--oooooh! Here's where I was testing the water; the one we actually soaked in was even nicer.
Thursday, June 14, 2007
Olympic wildlife
That was pretty cool. Two minutes later, however, something even more cool happened. Sitting beside the road was this blue grouse--obviously male and obviously trying to attract a mate--all puffed up, strutting around, and thrumming his tune. *I* was impressed!
He could run, too!
Hoping for a herd of elk and maybe a mountain lion tomorrow? :-)
Wednesday, June 13, 2007
Misty trails
Some eerily beautiful woods on the way up. Yep, this region gets a lot of rainfall...
A bit nippy on top! "Hurry up & take the photo already!" (Is that Mt. Baker in the back??)
View from the summit ridge. Clouds were in and out all day but yippee! -- no rain. I almost expected Heidi and her goats to go strolling by.
Today I ran ~3 hours on Kitsap Penninsula's Green Mountain and Gold Hill. These were predominantly mountain biking trails, but being midweek I had the trails mostly to myself. At one point I came around a corner and encountered a mama deer with her little fawn standing in the trail. Neither seemed particularly afraid of me, so I slowly scooted around them. I could have reached out and touched the fawn's nose--it was that close! Although sorely tempted, I did not try to touch it, of course. Alas, the camera was sitting in the car!
Will be back in the Olympics for most of the weekend. Hoh Rainforest, here we come! :) Hope everyone reading this has a great one!!
Monday, June 11, 2007
Wagonwheel Lake Trail
Trillium... most were white, but there were a couple of shades of purple here & there as well:
Spending the next few weeks in Washington State to climb mountains, run beautiful trails, and DRINK GOOD COFFEE! :) Ahh, yes... one of the things I love about WA is all the little drive-through espresso stands in the tiniest of towns. This is the bridge crossing the mouth of the Columbia River near Astoria, Oregon. And below is the quaint town of Astoria with the same bridge in the background:
And THIS is the Astoria Column , the top of which can be reached by climbing a circular staircase of 164 steps. Kind of reminded me of climbing the Leaning Tower of Pisa as a child... only this one was skinnier... and not leaning. The exterior artwork was pretty cool.
An interesting factoid: this is the area where Lewis & Clark wintered upon finally reaching the Pacific Ocean. The NPS has erected a "replica" of their fort on, or at least very near, the actual site, as well as a visitors' center, etc.


text:
Engine Starts? | |
Owner's Manual? | |
Free of Odors? |
Title Status | Outstanding Loan |
# of Keys | 2 |
Hassle-free Closing at a Swap Center
Skip the DMV! Swap Motors helps you with your title, tax, registration & other paperwork.
This Evaluation Report provides a condition summary of the vehicle and is not a comprehensive inspection.
Swap Motors recommends an ASE certified vehicle inspection prior to making a purchase.


text:
The Linear Book Scanner is a low-cost page-turning book scanner. The design is open-source, so anyone can build one.
A book moves back and forth over the machine.
Each time across, a vacuum sucks a page from one side to the other.
The pages are scanned as they travel across two imaging sensors.
The software produces a searchable PDF of your book!


text:
The Food for Health Issue: Also
- Abolish the Aisle: Would Divided Legislators Work Together If They Had to Sit in Alphabetical Order?
- Share
Abolish the Aisle: Would Divided Legislators Work Together If They Had to Sit in Alphabetical Order?
Marco Rubio would be next to Bernie Sanders, and Paul Ryan would rub elbows with Ohio Democrat Tim Ryan. If we closed the personal gap, maybe we could close the political one.
Everyone is looking for solutions to the gridlock that grips Washington. Most suggestions are complicated. But there's a simple step that could move our Congress in the right direction. We could stop seating the parties on opposite sides of the aisle.
The effects of the current seating arrangements are dramatically on display at every State of the Union address. One side rises in roaring applause for a point they agree with, as the other side sits in stony silence.
But what if talking with a legislator of a different party did not require "crossing the aisle?"
Helping legislators know their opponents as people rather than as positions could begin to build bridges across party lines.
It used to be that the folks we sent to represent us in Washington got to know each other across party lines. They went to the same social events, mixed at the annual White House Easter Egg Roll, and played golf together.
No more. Not only has the political gap widened, the personal gap has as well, and with it any semblance of trust. Members on opposite sides of the aisle hardly know each others' names, much less the names of their opponents' spouses or children.
Many factors contribute to the estrangement. With the onslaught of big money in politics, our legislators are occupied with fundraising from the moment they win their most recent election. They now regularly fly home on weekends. As E. Clay Shaw, a 26-year former Republican congressman from Florida, told the Sun Sentinel, "The Democrats and the Republicans don't really know each other. They get to town on a Tuesday morning, vote, and leave on a Thursday. They don't socialize. They never see each other."
Given the reduced opportunities for socializing in off-hours, perhaps it's time to encourage cross-party conversations during work hours. How? Simply rearrange the seating in Congress.
Imagine our legislators seated in alphabetical order.
In the House, Mitt Romney's running mate Paul Ryan would sit next to Ohio Democrat Tim Ryan. Progressive Pennsylvania Democrat Chaka Fattah would converse with Tea Party leader Stephen Fincher of Tennessee.
In the Senate, Republican Minority Leader Mitch McConnell would be sandwiched between progressive Democrats Claire McCaskill from Missouri and Robert Menendez from New Jersey. Firebrand Florida Republican Marco Rubio would get to know Vermont Independent Bernie Sanders.
Can a different seating arrangement make any difference?
Jim Kastama, a Democrat who served 16 years in the Washington state senate, says it can. In an op-ed in the Christian Science Monitor, Kastama recounts that when he joined the senate, there wasn't enough room on the Democrats' side of the chamber, so he had to sit with the Republicans. Later on, still a Democrat, he stayed on the Republican side by choice. Some accused him of being a Republican at heart and a sell-out. He claims his choice "… allowed me to build relationships with these lawmakers and their families, discover areas where we agree, and build trust. We still had sharp differences, but I found a willingness to work problems out, rather than fight."
The polarization we see today in our Congress has many sources. Gerrymandered districts create safe seats that enable extreme positions. The flood of money in politics funds politicians beholden to fixed positions. Our 24/7 media barrage thrives on demonization and polarizes us all.
Abolishing the aisle will not solve those problems. But helping legislators know their opponents as people rather than as positions could begin to build bridges across party lines. It doesn't cost money—and just might avoid the worst of the gridlock.
Fran Korten is a contributing editor for YES! Magazine, writing about opportunities to advance a progressive agenda in politics, economics, and the environment.


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Back In Town
Tonight is New Year's Eve, and I wish I could say I'm going into it ready to enjoy myself, but I'm experiencing lots of hesitation about my plans for the evening. I don't have super-high expectations of a special New Year's Eve; I learned long ago not to hope for "that magical night". But my plans involve dinner at Bricco Trattoria then celebrating at Rooftop120. I'm going to be with my siblings and some of my oldest friends. I need to be excited! But New Year's Eve out after trips to NYC and Florida, which happened to fall around the food-and-drink-heavy Christmas season, just has me thinking about calories. And the poor workout I will have the next day if I feel even the slightest bit hungover.
I got in almost all of the restaurant trips I wanted to this vacation. I already mentioned Chick-fil-A and Duffy's. I also got to have a healthy plant-based lunch at Christopher's Kitchen, got my Quizno's fix in the airport during the trip home, had a special dinner at Seasons 52 with my family, Kat, and her sister, and got to take my sister and good friend Greg to the Rum Bar for some tropical beverages. And though TooJay's was out of black and white cookies on Christmas Day, I got one later!


text:
Residential Construction Services
Do you feel as though you've outgrown your current home? There's no doubt that when you purchased it, the house was perfect for what you needed at the time, but now it's starting to feel a bit small for your liking. Or, perhaps you're a new homeowner and are looking to make a few changes to elevate your house to something that is perfectly tailored to your needs.
Whatever the reason, QualityRenos.com's residential construction services are a great option for anyone in the market for some changes to their existing home. With a reputation of providing clients with high quality work, QualityRenos.com is here to make your housing dreams come true.
Why Hire QualityRenos.com For Your Residential Construction Project?
At QualityRenos.com, we specialize in all areas of residential construction, from home additions to large scale renovation projects to the construction of custom homes.
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Kitchen Placement
My husband (born Jan, 1962, Metal Ox) and I (born Aug, 1972, Water Rat) are expecting our daughter in May this year and are moving, so we want to make sure the feng shui is correct for all 3 of us. Our problems are:
1) Kitchen Location
Our main door (in the West) opens into the vicinity of the kitchen (which is in the middle of the apartment). The kitchen has 2 doors - one that is opposite the main door (but not directly, more to the right) while the other one is directly opposite this first kitchen door and opens into the dining room (in the East).
We cannot relocate the kitchen, and all the books say it is bad to have a kitchen near the vicinity of the front door and also bad to have the kitchen in the middle of the home. What cures can we implement for both these problems?
As for your kitchen near the vicinity of the front door, so long as the kitchen door does not meet the main entrance door this is acceptable as most homes also face this problem.
As for the kitchn in the middle of the home, yes, this is considered not as auspicious. The most important thing here is to get the `Basics' of the kitchen correct:-
1. The location of your stove. This is important and where possible it should not have it's back against a window e.g. ventilation and best location is at the South part of the kitchen.
2. Next check that the stove and other `water' element e.g. wash basin are not close to each other. Leave a gap between them.
3. Forks, spoons, knifes MUST be kept in proper utility cupboards. This is particularly important in your case where the kitchen is at the centre of the house. Where possible keep the kitchen clean and tidy.
2) Bathrooms
We have 2 bathrooms (both which we need to use). The master bathroom is in the Northwest corner and the other (our soon-to-be-born baby daughter's) is in the Southwest corner. Her bedroom is also in this Southwest corner. What cures would you recommend so that we don't "flush" our helpful people and romance corners away? In addition, what cures and enhancers can we do for our daughter's bedroom so that we can bring her into the world with an auspicious start?
For the toilet in the SouthWest, traditional Feng Shui practise is that since it is your Romance corner, you should close the door and place the toilet lid down when not in use. In Feng Shui, such a `cure' or enhancement is of second importance as compared to the findings done with the Flying Star theory. The ranking of enhancements and cures would be based on such a ranking e.g. 1st if the Flying Star was done, this would be of top priority, next, 2nd priority goes to the use of the Eight House or 4 good or 4 bad, next after this then we can consider the Eight Aspirations or Life Aspirations Theory of e.g. SW is our `romance' corner.
At a micro level, even in a room, we can still activate the `SW' corner (provided it is a good area).
As for Feng Shui for children, I am not sure if you have read this short article I wrote: Feng Shui for Children Besides this article, do take note that preferably your soon-to-be-born baby daughter's room ideally should not be at the West location because such a room is considered warm. Other rooms are considered acceptable.
Cecil Lee
Center for Feng Shui Research
February 1999
- Hits: 93909


text:
Filters: Author is David P. Berners [Clear All Filters]
"The Switched Convolution Reverberator",
The Journal of the Audio Engineering Society, vol. 60, issue 4, pp. 227-236, 04/2012.
"The Switched Convolution Reverberator",
127th Audio Engineering Society Convention , New York, NY, USA, 10/2009.
"Doppler Simulation and the Leslie",
Proc. COST-G6 Conf. Digital Audio Effects (DAFx-02), Hamburg, Germany, vol. 20, no. 2, New York, CCRMA, Stanford University, pp. 13–20, September 26, 2002.
"CCRMA Papers Presented at the 1995 International Computer Music Conference, Banff, Canada",
International Computer Music Conference, no. STAN-M-91, Banff, Canada, 09/1995.


text:
The cookie dough is easy to make with a food processor. Whole wheat pastry flour, teff flour (or whole wheat flour), cocoa powder, muscovado sugar, salt, and baking soda were combined in the food processor and pulsed to mix. Cubed butter was dropped into the food processor one piece at a time while pulsing. The dough started becoming crumbly which is what you want it to do. Last, a little milk and vanilla extract were added while the machine was running, and the dough formed a ball. I wrapped the dough in plastic wrap and chilled it for about 30 minutes before rolling it out. For rolling, I placed the flattened dough between sheets of parchment paper since it was a little sticky. That way, extra flour wasn't worked into it while rolling. The dough was rolled to a little thicker than one quarter inch, and one and a half inch circles were cut. Although the yield for this recipe is noted as 40 cookies, I got exactly 24 circles even with re-rolling dough scraps. The cookies baked for about 10 minutes and were left to cool. The filling was made with butter, vanilla extract, a pinch of salt, and confectioners' sugar. There are recipes in the book for pantry items like homemade confectioners' sugar, vanilla and mint extracts, marshmallow crème, etc. I made the confectioners' sugar which was a simple matter of placing granulated sugar and cornstarch in the blender and letting it run for about five minutes. In the end, I didn't find the flavor much different from the organic confectioners' sugar I buy in bags, but it's nice to know I can whip some up if I find I have an empty bag and don't feel like going to the store. I used a plastic bag with the corner snipped to pipe the filling onto the flat sides of half the cookies. They were sandwiched and left to set up for about 10 minutes before a first taste.
If Oreos had always been this good, I would have been a huge fan. These were definitely worthy of being twisted apart for licking the middle, and they were great for biting right through to taste the cookie and filling at the same time. I want to try the Corn Chip Strips next or the Cheese Squares or maybe the homemade Nutter Butters. I'll definitely be making more homemade versions of snacks.
Crème-Stuffed Chocolate Cookies
Recipe excerpted from Real Snacks: Make Your Favorite Childhood Treats Without All the Junk by permission of Sasquatch Books. Copyright 2012 By Lara Ferroni. All rights reserved.
About 40 cookies
There are two types of people in this world: those who eat their Oreos in one piece and those who carefully twist them open to expose their delicious insides, lick them clean, and then dunk the dark chocolate wafers into milk until they are perfectly soggy. Guess which one I am.
1⁄2 cup (60 grams) whole- wheat pastry flour
1⁄4 cup (30 grams) teff or whole-wheat flour
1/3 cup (26 grams) cocoa powder
1⁄2 cup (100 grams) loosely packed muscavado or cane sugar
Pinch of salt
Pinch of baking soda
6 tablespoons (3⁄4 stick) unsalted butter
1 tablespoon whole milk
1 teaspoon vanilla extract
4 tablespoons (1⁄2 stick) unsalted butter, plus 2 tablespoons melted
1 teaspoon vanilla extract
Pinch of salt
1 cup (130 grams) powdered sugar
To make the cookies, combine the whole- wheat pastry flour, teff flour, cocoa powder, sugar, salt, and baking soda in the bowl of a food processor fitted with the dough blade (or in the bowl of a stand mixer fitted with the paddle attachment). Pulse several times to mix thoroughly. Drop in the butter 1 table- spoon at a time and pulse to create a crumbly mixture. Then, with the food processor running, drizzle in the milk and vanilla. Mix until the mixture starts to stick together, about 1 minute. Preheat the oven to 350°F. Line 2 baking sheets with parchment and set aside.
Turn the dough out onto a lightly floured surface. Roll out the dough until it is a little less than 1⁄4 inch thick. Use a 1- to 2-inch round cookie cutter to cut out the cookies (you can reroll any scraps). Place the cookies on the prepared baking sheet. Bake until set, 10 to 12 minutes. Allow the cookies to cool completely on a wire rack before filling. While the cookies are baking, make the filling. With a hand mixer or in the bowl of a stand mixer fitted with the paddle attachment, cream the butter, vanilla, and salt until smooth, about 1 minute. Gradually add the powdered sugar until fully incorporated.
Spread about 1 teaspoon of filling on half of the cooled cookies. Top with the remaining cookies and gently twist to seal. Let sit for 10 minutes before indulging.
For gluten-free Crème-Stuffed Chocolate Cookies, replace the whole-wheat pastry flour with an equal amount of gluten-free all- purpose baking mix. Roll out the dough between two pieces of parchment for easier rolling.
For vegan Crème-Stuffed Chocolate Cookies, replace the butter with an equal amount of coconut oil and the milk with an equal amount of almond milk.


text:
April 25, 2013
GROW workshop helps middle school girls learn crime-solving techniques
Submitted by Communications and Marketing
Middle school-age girls will help solve a mystery that has taken place on campus during a special session offered by Kansas State University's GROW, Girls Researching Our World, program.
CSI, Crime Scene Investigation will be from 9 a.m. to 3:30 p.m. Saturday, May 4. The special session and the GROW program are sponsored by the university's office for the advancement of women in science and engineering. GROW introduces middle school girls to the science, technology, engineering and mathematical fields.
University faculty and students from the colleges of Arts and Sciences, Engineering, Agriculture and Human Ecology will provide hands-on activities that show how people in forensic science fields use math, science and engineering to solve mysteries and crimes.
"Students will collect evidence from the crime scene to solve our own campus mystery," said Yasche Glass, program coordinator for the office for the advancement of women in science and engineering. "The ending will shock and amaze attendees and parents."
Registration is still open for this event until Wednesday, May 1.
For additional information, call Glass at 785-532-6088 or visit the GROW website.


text:
- BLIT (Block Transfer)
- Also known as BLT, blit refers to copying memory byte for byte. In C, this is referred to as a memcpy operation. The name originated with the BLT instruction on the DEC PDP-10 computer.
- CTFE (Compile Time Function Evaluation)
- Refers to the ability to execute regular D functions at compile time rather than at run time.
- code point
- In Unicode terminology, a code point is a logical character. The range of code points is 0 through 0x10FFFF. Only dchars can store code points directly; arrays of char and wchar need to use the variable-length encodings UTF-8 and UTF-16.
- COW (Copy On Write)
- COW is a memory management strategy where (usually large) reference objects are copied if they are to be modified. The constant overhead of COW can be high, but may be preferable in applications that would otherwise do a lot of preemptive copying.
- Data Race
- Two or more threads writing to the same memory location. Program behavior may depend on the arbitrary sequencing of these memory accesses.
- Functor
- A user-defined type (struct or class) that defines the function call operator (opCall in D) so it can be used similarly to a function.
- GC (Garbage Collection)
- Garbage collection is the common name for the term automatic memory management. Memory can be allocated and used, and the GC will automatically free any chunks of memory no longer referred to. In contrast, explicit memory management is where the programmer must carefully match up each allocation with one and only one call to free().
- Higher-order function
- A function that either accepts another function as a parameter, returns a function, or both.
- IFTI (Implicit Function Template Instantiation)
- Refers to the ability to instantiate a template function without having to explicitly pass in the types to the template. Instead, the types are infered automatically from the types of the runtime arguments.
- Illegal
- A code construct is illegal if it does not conform to the D language specification. This may be true even if the compiler or runtime fails to detect the error.
- Input range
- A type (i.e., a struct or a class) that defines the member functions empty, head, and next. Input ranges are assumed to be strictly one-pass: there is no way to save the state of the iteration in a copy of the range. See also range.
- Implementation Defined Behavior
- This is variation in behavior of the D language in a manner that is up to the implementor of the language. An example of implementation defined behavior would be the size in bytes of a pointer: on a 32 bit machine it would be 4 bytes, on a 64 bit machine it would be 8 bytes. Minimizing implementation defined behavior in the language will maximize the portability of code.
- lvalue
- An lvalue is an abstract term referring to a value with an accessible address (through e.g. the unary & operator). Typical examples of lvalues include variables, constants introduced with const or immutable (but not enum), and elements of arrays and associative arrays. Calls to functions that return ref and pointer dereference expressions are also lvalues. Lvalues can be passed to (or be returned from) functions by reference. An lvalue is the counterpart to an rvalue.
- NRVO (Named Return Value Optimization)
NRVO is a technique invented by Walter Bright around 1991 (the term for it was coined later) to minimize copying of struct data. Functions normally return their function return values in registers. For structs, however, they often are too big to fit in registers. The usual solution to this is to pass to the function a hidden pointer to a struct instance in the caller's stack frame, and the return value is copied there. For example:
struct S { int a, b, c, d; } S foo() { S result; result.a = 3; return result; } void test() { S s = foo(); }
is rewritten as:
S* foo(S* hidden) { S result; result.a = 3; *hidden = result; return hidden; } void test() { S tmp; S s = *foo(&tmp); }
This rewrite gives us an extra temporary object tmp, and copies the struct contents twice. What NRVO does is recognize that the sole purpose of result is to provide a return value, and so all references to result can be replaced with *hidden. foo is then rewritten as:
S* foo(S* hidden) { hidden.a = 3; return hidden; }
A further optimization is done on the call to foo to eliminate the other copy, giving:
void test() { S s; foo(&s); }
The result is written directly into the destination s, instead of passing through two other instances.
- narrow strings
- All arrays that use char, wchar, and their qualified versions are narrow strings. (Those include string and wstring). Range-oriented functions in the standard library handle narrow strings specially by automatically decoding the UTF-encoded characters.
- opApply
- A special member function used to iterate over a collection; this is used by the ForeachStatement.
- opApplyReverse
- A special member function used to iterate over a collection in the reverse order; this is used by the ForeachStatement.
- POD (Plain Old Data)
- Refers to a struct that contains no hidden members, does not have virtual functions, does not inherit, has no destructor, and can be initialized and copied via simple bit copies.
- Predicate
- A function or delegate returning a Boolean result. Predicates can be nullary (take no arguments), unary (take one argument), binary (take two arguments), or n-ary (take n arguments). Usually predicates are mentioned within the context of higher-order functions, which accept predicates as parameters.
- RAII (Resource Acquisition Is Initialization)
- RAII refers to the technique of having the destructor of a class object called when the object goes out of scope. The destructor then releases any resources acquired by that object. RAII is commonly used for resources that are in short supply or that must have a predictable point when they are released. RAII objects in D are created using the scope storage class.
- rvalue
- An rvalue is an abstract term referring to a value resulting from an expression that has no accessible memory address. This lack of address is only conceptual; the implementation has the liberty to store an rvalue in addressable memory. Rvalues cannot be changed and cannot be passed to (or be returned from) functions by reference. An rvalue is the counterpart to an lvalue.
- Sequential Consistency
- Data being written in one order in one thread being visible in the same order to another thread.
- SFINAE (Substitution Failure Is Not An Error)
- If template argument deduction results in a type that is not valid, that specialization of the template is not considered further. It is not a compile error. See also SFINAE.
- TMP (Template Metaprogramming)
- TMP is using the template features of the language to execute programs at compile time rather than runtime.
- TLS (Thread Local Storage)
- TLS allocates each thread its own instance of the global data. See also Wikipedia.
- UB (Undefined Behavior)
- Undefined behavior happens when an illegal code construct is executed. Undefined behavior can include random, erratic results, crashes, faulting, etc. A buffer overflow is an example of undefined behavior.
- UDA (User-Defined Attribute)
- User-defined attributes are metadata attached to symbols used for compile-time reflection.
- UFCS (Uniform Function Call Syntax)
- Uniform function call syntax refers to the ability to call a function as if it were a method of its first argument. For example funct(arg) may be written as arg.funct().


text:
Persons using assistive technology might not be able to fully access information in this file. For assistance, please send e-mail to: firstname.lastname@example.com. Type 508 Accommodation and the title of the report in the subject line of e-mail. Recommended Childhood and Adolescent Immunization Schedule --- United States, 2006Harmonized Childhood and Adolescent Immunization Schedule, 2006The Advisory Committee on Immunization Practices (ACIP) periodically reviews the recommended childhood and adolescent immunization schedule to ensure that the schedule is current with changes in vaccine formulations and reflects revised recommendations for the use of licensed vaccines, including those newly licensed. The recommendations and format of the childhood and adolescent immunization schedule and catch-up schedule for January--December 2006 were approved by ACIP, the American Academy of Pediatrics (AAP), and the American Academy of Family Physicians (AAFP) (Figure and Table). The changes to the previous childhood and adolescent immunization schedule, published January 2005 (1), are as follows:
Vaccine Information StatementsThe National Childhood Vaccine Injury Act requires that health-care providers provide parents or patients with copies of Vaccine Information Statements before administering each dose of the vaccines listed in the schedule. Additional information is available from state health departments and from CDC at http://www.cdc.gov/nip/publications/vis. Detailed recommendations for using vaccines are available from package inserts, ACIP statements on specific vaccines, and the 2003 Red Book (5). ACIP statements for each recommended childhood vaccine are available at the CDC National Immunization Program website at http://www.cdc.gov/nip/publications/acip-list.htm. In addition, guidance for obtaining and completing a Vaccine Adverse Event Reporting System form is available at http://www.vaers.hhs.gov or by telephone, 800-822-7967. References
The Recommended Childhood and Adolescent Immunization Schedule and the Catch-up Childhood and Adolescent Immunization Schedule have been approved by the Advisory Committee on Immunization Practices, the American Academy of Pediatrics, and the American Academy of Family Physicians. The standard MMWR footnote format has been modified for publication of this schedule. Suggested citation: Centers for Disease Control and Prevention. Recommended childhood and adolescent immunization schedule---United States, 2006. MMWR 2005;54 (Nos. 51&52):Q1--Q4. Figure Return to top. Table Return to top.
Disclaimer All MMWR HTML versions of articles are electronic conversions from ASCII text into HTML. This conversion may have resulted in character translation or format errors in the HTML version. Users should not rely on this HTML document, but are referred to the electronic PDF version and/or the original MMWR paper copy for the official text, figures, and tables. An original paper copy of this issue can be obtained from the Superintendent of Documents, U.S. Government Printing Office (GPO), Washington, DC 20402-9371; telephone: (202) 512-1800. Contact GPO for current prices. **Questions or messages regarding errors in formatting should be addressed to firstname.lastname@example.com last reviewed: 1/5/2006 |


text:
GEO Publications
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GEO accession:
Sample GSM3388539
Query DataSets for GSM3388539
Public on Feb 20, 2019
Sample type
Source name
mouse embryos
Mus musculus
developmental stage: mouse embryo (E9.5, E10.5, E11.5, E12.5, E13.5)
genetic background: B6 background
Growth protocol
The C57BL/6 mice were obtained from The Jackson Laboratory (Bar Harbor, ME) and plug matings were set up. Noon on the day of the vaginal plug was considered as embryonic day (E) 0.5. Dissections were done as previously described76 and all embryos were immediately snap frozen in liquid nitrogen. All animal procedures were in accordance with institutional, state, and government regulations (IACUC protocol 4378-01).
Extracted molecule
polyA RNA
Extraction protocol
Mouse embryos from different development stages were processed together to reduce batch effect. Each mouse embryo was minced into small pieces by blade in 1 mL ice-cold cell lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630 from77, modified to also include 1% SUPERase In and 1% BSA) and transferred to the top of a 40um cell strainer (Falcon). Tissues were homogenized with the rubber tip of a syringe plunger (5ml, BD) in 4ml cell lysis buffer. The filtered nuclei were then transferred to a new 15ml tube (Falcon) and pelleted by centrifuge at 500xg for 5min and washed once with 1ml cell lysis buffer. The nuclei were fixed in 4ml ice cold 4% paraformaldehyde (EMS) for 15min on ice. After fixation, the nuclei were washed twice in 1ml nuclei wash buffer (cell lysis buffer without IGEPAL), and re-suspended in 500ul nuclei wash buffer. The samples were split to two tubes with 250ul in each tube and flash frozen in liquid nitrogen. We estimated the nuclei extraction efficiency based on extracted nuclei number vs. expected total nuclei number in each embryo and the nuclei extraction efficiency range from 60% to 85%.
Thawed nuclei are permeabilized with 0.2% tritonX-100 (in nuclei wash buffer) for 3 minutes on ice, and briefly sonicated (Diagenode, 12s on low power mode) to reduce nuclei clumping. The nuclei were then washed once with nuclei wash buffer and filtered through 1ml Flowmi cell strainer (Flowmi). Filtered nuclei were spun down at 500xg for 5min and resuspended in nuclei wash buffer. Nuclei from each mouse embryo were then distributed into several individual wells in four 96-well plates. The links between well id and mouse embryo were recorded for downstream data processing. For each well, 80,000 nuclei (16 µL) were mixed with 8 µl of 25 µM anchored oligo-dT primer (5′- /5Phos/CAGAGCNNNNNNNN[10bp barcode]TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-3′, where "N" is any base; IDT) and 2 µL 10 mM dNTP mix (Thermo), denatured at 55°C for 5 min and immediately placed on ice. 14 µL of first-strand reaction mix, containing 8 µL 5X Superscript IV First-Strand Buffer (Invitrogen), 2 µl 100 mM DTT (Invitrogen), 2 µl SuperScript IV reverse transcriptase (200 U/μl, Invitrogen), 2 μL RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen), was then added to each well. Reverse transcription was carried out by incubating plates by gradient temperature (4°C 2 minutes, 10°C 2 minutes, 20°C 2 minutes, 30°C 2 minutes, 40°C 2 minutes, 50°C 2 minutes and 55°C 10 minutes). After ligation reaction, 60µL nuclei dilution buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 1% BSA) was added into each well. Nuclei from all wells were pooled together and spun down at 500xg for 10min. Nuclei were then resuspended in nuclei wash buffer and redistributed into another four 96-well plates with each well including 4µL T4 ligation buffer (NEB), 2µL T4 DNA ligase (NEB), 4µL Betaine solution (5M, Sigma-Aldrich), 6µL nuclei in nuclei wash buffer, 8µL barcoded ligation adaptor (100uM, 5'- GCTCTG[9bp or 10bp barcode A]/ideoxyU/ACGACGCTCTTCCGATCT[reverse complement of barcode A]-3') and 16µL 40% PEG 8000 (Sigma-Aldrich). The ligation reaction was done at 16°C for 3 hours. After RT reaction, 60µL nuclei dilution buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 1% BSA) was added into each well. Nuclei from all wells were pooled together and spun down at 600xg for 10min. Nuclei were washed once with nuclei wash buffer and filtered with 1ml Flowmi cell strainer (Flowmi) twice, counted and redistributed into eight 96-well plates with each well including 2,500 nuclei in 5µL nuclei wash buffer and 5µL elution buffer (Qiagen). 1.33 μl mRNA Second Strand Synthesis buffer (NEB) and 0.66 μl mRNA Second Strand Synthesis enzyme (NEB) were then added to each well, and second strand synthesis was carried out at 16°C for 180 min. For tagmentation, each well was mixed with 11 μL Nextera TD buffer (Illumina) and 1 μL i7 only TDE1 enyzme (62.5nM, Illumina), and then incubated at 55°C for 5 min to carry out tagmentation. The reaction was then stopped by adding 24 μL DNA binding buffer (Zymo) per well and incubating at room temperature for 5 min. Each well was then purified using 1.5x AMPure XP beads (Beckman Coulter). In the elution step, each well was added with 8µL nuclease free water, 1µL 10X USER buffer (NEB), 1µL USER enzyme (NEB) and incubated at 37°C for 15 min. Another 6.5µL elution buffer was added into each well. The AMPure XP beads were removed by magnetic stand and the elution product was transferred into a new 96-well plate. For PCR amplification, each well (16µL product) was mixed with 2μL of 10 μM indexed P5 primer (5′-AATGATACGGCGACCACCGAGATCTACAC[i5]ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′; IDT), 2 μL of 10 μM P7 primer (5′-CAAGCAGAAGACGGCATACGAGAT[i7]GTCTCGTGGGCTCGG-3′, IDT), and 20 μL NEBNext High-Fidelity 2X PCR Master Mix (NEB). Amplification was carried out using the following program: 72°C for 5 min, 98°C for 30 sec, 12-14 cycles of (98°C for 10 sec, 66°C for 30 sec, 72°C for 1 min) and a final 72°C for 5 min.
Library strategy
Library source
Library selection
Instrument model
Illumina NovaSeq 6000
sci-RNA-seq3 library
Data processing
For sc-RNA-seq processing, read alignment and gene count matrix generation for single cell RNA-seq of co-assay was the same with sci-RNA-seq ("J. Cao et al., Comprehensive single cell transcriptional profiling of a multicellular organism by combinatorial indexing. bioRxiv, 104844 (2017).") with minor modifications: Reads were mapped to a chimeric reference genome of human (hg19) and mouse (mm10) for RNA-seq from A594, HEK293T and NIH/3T3 cells, and mouse (mm10) only for RNA-seq from mouse kidney experiment with STAR/v 2.5.2b, with gene annotations (GENCODE V19 for human; GENCODE VM11 for mouse). For mixed-species experiment, the percentage of uniquely mapping reads for genomes of each species was calculated. Cells with over 80% of UMIs assigned to one species were regarded as species-specific cells, with the remaining cells classified as mixed cells or "collisions".
Genome_build: mouse reference genome (mm10)
Supplementary_files_format_and_content: Processed data files include a cell annotation csv file, gene annotation csv file, and a gene count sparse matrix file
Supplementary_files_format_and_content: Cell annotation file: sample is cell id of each single cell with the reverse transcription and ligation barcode attached; tsne_1 and tsne_2 are cell coordinates after t-SNE dimension reduction.
Supplementary_files_format_and_content: Gene annotation file including gene id, gene type and gene short name.
Supplementary_files_format_and_content: A sparse matrix file: each row corresponds to gene id (with gene information in the gene_annotation.csv; each column corresponds to each cell (with cell source information in the cell_annotation.csv); each value in the matrix corresponds to UMI count.
Submission date
Sep 13, 2018
Last update date
Feb 20, 2019
Contact name
Junyue Cao
Organization name
University of Washington
Department of Genome Sciences
Shendure lab
Street address
Foege Building S-210, 3720 15th Ave NE
ZIP/Postal code
Platform ID
Series (1)
The dynamic transcriptional landscape of mammalian organogenesis at single cell resolution
Supplementary data files not provided
SRA Run Selector
Raw data are available in SRA
Processed data are available on Series record
GEO Help


text:
Nitrous oxide is commonly associated with laughing gas—the pleasantly benign vapor that puts patients at ease in the dentist's chair. But outside the dentist's office, the gas plays a serious role in the planet's warming climate.
After carbon dioxide and methane, nitrous oxide is the third-largest contributor of greenhouse-gas emissions to the atmosphere. The colorless gas is also the top culprit in the depletion of ozone—the layer of the atmosphere that protects Earth from the sun's ultraviolet radiation.
The majority of nitrous oxide emissions arise naturally from soil, where microbes break down nitrogen, releasing nitrous oxide as a byproduct. However, human activities such as farming, and the use of fertilizer, in particular, have increased nitrous oxide emissions over the last 35 years—a rise that has contributed to the overall warming of the planet.
Now scientists in Massachusetts Institute of Technology (MIT)'s Center for Global Change Science have developed a highly detailed model that simulates levels of nitrous oxide emissions in different regions and ecosystems of the world. Based on local soil temperature and moisture content, some of the simulations were able to reproduce actual nitrous oxide measurements.
From their simulations, the researchers discovered a surprising pattern: Regions around the world typically experience a decrease in nitrous oxide emissions during El Niño events, which periodically create unusually warm waters in the Pacific Ocean, affecting temperature and rainfall patterns around the world. Conversely, they found that emissions rise during periods of La Niña, the opposing weather pattern, in which colder waters take over the Pacific. The findings suggest a feedback mechanism in which nitrous oxide not only contributes to global warming, but may also be affected by climate patterns.
"If more emissions are released into the atmosphere, there will be more global warming … and with higher temperatures, we would have more nitrous oxide coming out," says Eri Saikawa, who led the research as a postdoctoral researcher at MIT. "Many people may not consider the nitrogen cycle, but we do have to realize it is pretty important."
Saikawa, who is now an asst. prof. of environmental studies at Emory Univ., collaborated with Ron Prinn, the TEPCO Prof. of Atmospheric Science at MIT and director of the Center for Global Change Science, as well as principal research scientist Adam Schlosser. The group published its results in Global Biogeochemical Cycles.
A seesaw of emissions
To simulate nitrous oxide emissions around the world, Saikawa adapted a model of soil temperature and moisture content that is often used by hydrologists to track the movement of water through soil.
Saikawa added to this model a component that calculates how much nitrous oxide is likely emitted from a region, given variables such as soil temperature and moisture. She simulated monthly global nitrous oxide emissions from 1975 to 2008; to check that the model generated accurate calculations, Saikawa simulated nitrous oxide emissions in regions where actual nitrous oxide measurements were available, including 25 locations in the Amazon, North and Central America, Asia, Africa and Europe.
For many of the sites, the model's calculations agreed with observations, verifying its ability to accurately simulate nitrous oxide emissions. Looking at the variability of emissions from year to year, Saikawa noticed a dramatic correlation with the El Niño/La Niña climate pattern, particularly in tropical regions near the equator: Nitrous oxide emissions dipped during periods of El Niño, and spiked during La Niña events.
Saikawa says this periodic seesaw in emissions makes sense: As El Niño warms the Pacific, rainfall increases to the east, causing flooding in parts of South America, and droughts in parts of South Asia. Saikawa points out that the largest sources of nitrous oxide emissions arise from South Asia; Saikawa observed that decreased soil moisture from El Niño led to a large dip in emissions from those regions, with the opposite effect from La Niña.
Modeling better fertilizer
Going forward, the team will incorporate agricultural components into the model, to simulate the effect of certain fertilizers on nitrous oxide emissions. Many types of fertilizer introduce nitrogen to the soil—an ingredient that nitrogen bacteria thrive upon. The more fertilizer nitrogen there is in soil, the more bacteria break it down, releasing nitrous oxide as a byproduct.
Prinn says that deforestation has also stirred up nitrous oxide emissions, particularly in regions such as Brazil. The Brazilian government, he says, is exploring the increased production of biofuels, fertilizing croplands in place of forests.
"Brazil and other countries are very concerned about the sustainable production of biofuels in the future," Prinn says. "What damage will it do to soil health? … Will they be making biofuels that are causing nitrous oxide and carbon dioxide emissions?"
A model like Saikawa's, he says, may help simulate the effect of biofuel production on nitrous oxide emissions, and present more sustainable methods for growing biofuel crops.


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Behind the Scenes - Animating a Title
Written by
on .As everyone knows, OpenShot has a new 3D animated title system. However, what you might not know, is exactly how Blender is being used to create these animations. I thought I would explain what goes into creating a new Blender title sequence.
When using OpenShot, the interface is super simple. Take a look at the window I used to create the titles for this video. Each animated curve has a color picker, so adjusting the colors is quick and easy. The glare and lighting in the video are affected by the different colors, so choose wisely, and create your own stunning combination.


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General Terms and Conditions of In-Store Sale applicable from October 25th 2019 to October 19th 2022
GENERAL TERMS AND CONDITIONS OF IN-STORE SALE APPLICABLE AS FROM JANUARY 01st 2024 : CLICK HERE
These general terms and conditions of in-store sale (hereinafter the "Terms and Conditions") apply, excluding all other documents, to all purchases on the customer's own behalf (hereinafter the "Customer") in relation to MESSIKA products (hereinafter the "Products" or "Product"), performed within any of the stores operated in its own right by MESSIKA GROUP (hereinafter the "Store"), namely the Store located on 259 Rue Saint-Honoré, 75001 Paris. Any contrary condition raised by the Customer shall, unless expressly accepted by the Store, be unenforceable against it. Specific conditions may however apply to a specific transaction or offer proposed to Customers in the Store, which in such case shall take precedence over these Terms and Conditions.
They determine the rights and obligations of the parties in the context of the sale of Products (hereinafter the "Sale").
Sales performed in this context are reserved for consumers only, and any purchase for resale on a professional basis is strictly prohibited.
These Terms and Conditions are not intended to cover the purchase of products on MESSIKA's website.
These Terms and Conditions do not apply to sales of custom products which are governed by the special terms and conditions communicated at such time and available by request.
These Terms and Conditions may be modified at any time by MESSIKA GROUP as the vendor identified in article 2 of these terms and conditions.
The Terms and Conditions applicable are those in force at the time of Sale.
Products are sold by the company: MESSIKA GROUP, SAS [simplified joint-stock company] with share capital of €1,100,000, whose registered address is 64, rue La Fayette, 75009 PARIS, registered with the Paris Trade and Companies Register under number 301 293 999, holding EU VAT number FR22301293999 (hereinafter "MESSIKA").
The basic characteristics of the Products as well as prices are displayed on a specific label or display in the Store.
The price of Products is expressed in Euros, including tax, namely value-added tax at the legal rate in force.
Any purchase of a ring of a size smaller than 48 or larger than 60 shall be considered to order as defined in article 5, "Returns". This order must be the subject of a prior quote, and may not be returned (see articles 5.2.2 "Products not accepted for returns" and 5.3 "Conditions for issue of a credit note").
MESSIKA reserves the right to limit the quantities of MESSIKA products purchased by a Customer, and/or refuse any unusual or bad-faith transaction, where this would constitute "due cause" according to article L.121-11 of the Consumer Code.
Purchases must be paid for in full on the day of purchase, directly in the Store.
The Customer may pay for MESSIKA products by cash, bank/credit card or bank transfer.
Payment by cheque is not accepted.
In the event that, for any reason whatsoever (objection, refusal by issuer, etc.), the sums owed by the Customer to the Store cannot be debited, the purchase process shall be cancelled. MESSIKA will not issue the product to the Customer without validation of the Customer's payment.
The Customer guarantees that they have the necessary authorisation to use the selected payment method for the payment of their purchase.
4.1. Payment in cash
The Store accepts payment in cash up to the limits set by French regulations:
- The legal threshold for payment in cash authorised for French tax residents is €1,000.
- The legal threshold for payment in cash authorised for non-French tax residents is €15,000.
Any purchase of an amount greater than these thresholds must be paid for by non-cash payment methods (bank transfer or payment/credit cards).
4.2. Payment by bank card
The Store accepts "CB" cards, Visa, Eurocard/Mastercard, American Express, China Union Pay, Japan Credit Bureau, WeChat Pay, Alipay.
4.3.Bank Transfer
The Store accepts payment by transfer received in Euros to our bank account registered in France.
MESSIKA's bank must confirm receipt of the transfer before considering that payment has been made.
5.1. Refunds
MESSIKA will not refund any Product purchased in the Store.
5.2.Returns Policy
MESSIKA wishes to offer its Customers the option to return purchased Products under the following conditions.
5.2.1.Return conditions
MESSIKA allows its Customers to return purchased Products, so long as the following cumulative conditions are met:
- The exchange must be requested within a maximum of 30 days following the date of the invoice;
- The return request must be addressed to the Store in which the Product was purchased and the return can only be carried out there;
- The returned Product must be brand new, unused (not worn or engraved). Upon return of the Product, MESSIKA's quality control will assess its condition;
- The product must be returned with:
- The original box, fully intact,
- The GIA certificate(s) issued upon purchase,
- The guarantee card
5.2.2.Products not accepted for returns
Messika reserves the right to refuse to take back a Product in the following cases:
- Returned products that are incomplete, damaged, deteriorated, soiled or in any other condition that would reasonably suggest that they have been used or worn;
- Products that have been the subject of a return request more than twice successively;
- Products that were specially ordered such as the creation of an item at the Customer's request;
- Products that were made to order such as ordering an unavailable item or in an unavailable size;
- Products customised to order such as engraved or modified items.
Subject to compliance with the return conditions provided in article 5.2.1, MESSIKA will provide an exchange as soon as possible.
- Exchanges only permit the returned product to be replaced with the same model in a different colour of gold and/or size.
- In the event of an increase in retail price between the date of purchase and the date of exchange, the Customer must pay the price difference.
- It is specified that in cases of gifts, the recipient of the gift may exchange it.
5.4.Credit notes
5.4.1. Conditions for issue of a credit note
If the Customer wishes to return their purchase for another model or if the product cannot be exchanged due to an unavailability of the Customer's desired size or model, a credit note may be issued if:
- The product meets the conditions provided in article 5.2.1;
- The product is not included in the cases provided in article 5.2.2.
5.4.2. How a credit note is issued and used
If the product is declared compliant, MESSIKA will issue a credit note.
The credit note is personal and valid for one (1) year from its issue date, exclusively in the Store in which the purchase was made.
For gifts, the credit note cannot be issued in the recipient's name, but the Customer's name only.
The credit note cannot be swapped for cash or bought back, replaced or refunded if lost, stolen, illegible or expired. MESSIKA shall not be held responsible in the event of fraudulent use of the credit note by a third party.
Depending on technical feasibility, the Product may be refurbished by MESSIKA, at the Customer's expense, upon presentation of receipt of purchase and the certificate of authenticity.
MESSIKA products benefit from: - the legal guarantee of conformity provided in articles L.217-4 and following of the Consumer Code. Thus if the product does not comply with the description given in the Store or is not suitable for the expected use, the Customer has 2 years from the delivery of the product to invoke this guarantee and, according to their choice, have it repaired or replaced (subject to the cost conditions provided in article L.217-9 of the Consumer Code). - the guarantee against hidden defects provided in articles 1641 to 1649 of the Civil Code. As a result, if the product is not suitable for its intended use or its use is diminished such that the Customer would not have bought it, due to hidden defects from the time prior to purchase, they have 2 years from discovery of the defect to act on the basis of the guarantee against hidden defects. If a hidden defect is found the Customer may choose between having the price of the product refunded or reduced according to article 1644 of the Civil Code. |
To recall articles L.217-4 and L.211-12 of the Consumer Code, and articles 1641 and 1648, first paragraph, of the Civil Code:
"Article L.217-4 of the Consumer Code: the seller delivers an item according to the contract and is responsible for compliance faults existing upon delivery. It is also responsible for compliance faults resulting from packaging, assembly instructions or installation when these have been made its responsibility according to the contract or been carried out under its responsibility."
"Article L.217-4 of the Consumer Code: the item complies with the contract:
- If it is suited to the use usually expected of a similar product, and where applicable:
- if it corresponds to the description given by the seller and has the qualities that it has presented to the buyer in the form of a sample or model;
- if it has the qualities that a buyer may reasonably expect with regard to the public declarations by the seller, the manufacturer or its representative, particularly in its advertising or labelling;
- Or, if it has the characteristics mutually defined by the parties or is specific to a special use sought by the buyer, brought to the seller's knowledge and accepted by it."
"Article L.217-12: Action arising from a compliance fault is time-limited to two years from the delivery of the item."
"Article 1641 of the Civil Code: The seller is bound by the guarantee against hidden defects in the sold item making it unsuitable for the intended use, or which so diminish its use that the Customer would not have bought it, or would have offered a lower price, if it had been aware of them."
"Article 1648 of the Civil Code, first paragraph: Action resulting from critical defects must be brought by the buyer within two years from the discovery of the defect."
All Products purchased in MESSIKA Stores are provided with the MESSIKA guarantee.
MESSIKA jewellery has a two-year guarantee from the date of the receipt if a compliance or hidden defect is discovered under normal conditions of use. The MESSIKA guarantee card accompanying the Product at purchase must also be presented.
Any Product returned during the guarantee period in order to avail of the guarantee shall be submitted to MESSIKA's after sales service's appraisal. If they find a defective element, or a manufacturing fault, the Product will be repaired or replaced by MESSIKA.
A third year of the guarantee is offered to the Customer if they register after having completed the form available on our website, messika.com, within the month following purchase. It is valid for all MESSIKA jewellery.
The guarantor of the jewellery is the company MESSIKA, whose registered address is 64, Rue Lafayette in Paris (75009). For the implementation of the commercial guarantee, the Customer must address MESSIKA's customer service at the following email address: firstname.lastname@example.com.
The MESSIKA Guarantee excludes:
- Faults or damage resulting from incorrect use or accidents;
- Faults or damage caused by inappropriate use (shocks, scratches, crushing), by alterations, dismantling, unauthorised repairs, as well as services, intervention or repairs carried out other than in a MESSIKA store or approved retailer. For example, in case of adapting size, replacing stones or polishing;
- The consequences of normal wear and tear of the jewellery.
Independently of the commercial guarantee, Messika remains bound by the legal compliance guarantee mentioned in articles L.217-4 to L.217-12 of the Consumer Code, as well as those relative to defects in the item sold, under the conditions of articles 1641 to 1648 and 2232 of the Civil Code.
Legal provisions:
Article L.217-4 of the Consumer Code
The seller delivers an item according to the contract and is responsible for compliance faults existing upon delivery.
It is also responsible for compliance faults resulting from packaging, assembly instructions or installation when these have been made its responsibility according to the contract or been carried out under its responsibility.
Article L.217-4 of the Consumer Code
The item complies with the contract:
- If it is suited to the use usually expected of a similar product, and where applicable:
- if it corresponds to the description given by the seller and has the qualities that it has presented to the buyer in the form of a sample or model;
- if it has the qualities that a buyer may reasonably expect with regard to the public declarations by the seller, the manufacturer or its representative, particularly in its advertising or labelling;
- Or, if it has the characteristics mutually defined by the parties or is specific to a special use sought by the buyer, brought to the seller's knowledge and accepted by it."
Article L.217-12 of the Consumer Code
Action arising from a compliance fault is time-limited to two years from the delivery of the item.
Article L.217-16 of the Consumer Code
Where the buyer requests of the seller, during the term of the commercial guarantee that has been granted upon purchase or repair of an item, a refurbishment covered by the guarantee, any immobilisation period of at least seven days will be added to the length of the guarantee remaining.
This period runs from the request for intervention by the buyer or provision of the item for repair, if this is subsequent to the request for intervention.
The personal data communicated by the Customer to MESSIKA in the Store will be collected and processed by MESSIKA, in its capacity as data controller, according to the provisions of the IT and Liberties Law no. 78-17 of 6 January 1978 as amended and the European General Data Protection Regulation which entered into force on 25 May 2018. Data is conserved throughout the term of the commercial relationship, then archived for the purposes of legal proof.
It will be processed for the purposes of managing Customer orders, deliveries, guarantees applicable to products that the Customer purchases and the execution of payment orders. It will also be used for the purposes of management and optimisation of customer relations and statistics. It may be communicated (i) to MESSIKA's internal services and/or (ii) any of MESSIKA's subcontractors or service providers, where such disclosure is necessary for the accomplishment of the abovementioned purposes.
Data will in no case be transferred outside the European Union. This data will not be externally communicated other than as stated above except to comply with legal and regulatory obligations or the request of a legal authority or administration. MESSIKA guarantees that it implements optimal security measures against loss, alteration and unauthorised communication of data transmitted by the Customer.
The Customer may exercise their rights of access, rectification, portability, erasure, objection and limitation of the processing of their personal data, or withdraw their consent, by contacting our Customer Service by email at firstname.lastname@example.com. The Customer is also informed that it has the right to bring a claim before the competent control authority, such as the CNIL.
For further information on our practices on protecting Customer data, the Store invites the Customer to consult the MESSIKA GROUP's Personal Data policy, accessible on our website www.messika.com.
Further, the Customer may register on a list to be excluded from telephone solicitation: http://www.bloctel.gouv.fr/.
For all information, claims, invoking of legal guarantees or questions about the conditions of sale of MESSIKA products or those products themselves, the Customer may contact Customer Services by email at email@example.com
MESSIKA remains the owner of all intellectual property for photographs, presentations, studies, sketches, models, prototypes, etc. created (even at the Customer's request) for the provision of products. The Customer is therefore prohibited from any reproduction or exploitation of those photographs, presentations, studies, sketches, models, prototypes, etc. without prior express permission in writing from MESSIKA which may condition it against financial compensation.
All diamonds purchased by MESSIKA originate from legitimate sources not involved in the financing of conflict, according to the resolutions of the United Nations. MESSIKA hereby guarantees that the diamonds are not sourced from conflict zones, this guarantee being based on its knowledge and/or guarantees in writing from the supplier of the diamonds.
The execution of MESSIKA's obligations hereunder is suspended in the event of the occurrence of a fortuitous or force majeure event preventing such execution.
The suspension of its obligations may in no case be a cause for responsibility due to non-execution of that obligation, or incur any payment of damages or late penalties.
The execution of the obligation is suspended throughout the duration of the force majeure if it is temporary and does not exceed a period of eight (8) days.
If the prevention is definitive or exceeds that period of eight (8) days, these Terms and Conditions shall be simply rescinded.
Our TERMS AND CONDITIONS are governed by and subject to French law.
In accordance with article L.211-3 of the Consumer Code, any consumer-related dispute may be amicably settled by mediation with the CMAP – Paris Arbitration and Mediation Centre.
In order to refer the dispute to the mediator, the Customer may (i) complete the form on the CMAP website: (www.mediateur-conso.cmap.fr), (ii) send the request by simple or registered letter to CMAP Médiation Consommation, 39 avenue Franklin D. Roosevelt, 75008 PARIS, or (iii) email firstname.lastname@example.com. Irrespective of the means used to contact the CMAP, the request must contain the following in order to be processed promptly: the Customer's postal address, email and telephone details as well as MESSIKA's full name and address, a clear summary of the facts, and proof of the measures already taken with MESSIKA.
If the Customer does not wish to do so, the dispute will be referred to the French courts.


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Look to the woods, there you will see a silvery female staring at you with silver grey eyes. If she comes to you, you will be able to meet the pack behind her.
silver-crow-pack, look, woods, there, will, silvery, female, staring, with, silver, grey, eyes, comes, able, meet, pack, behind
a location to play the deeper more story focused RPG games.
dream, play, post, game, horror, story, gamming, nightmares, death, skull, #crow, dreaming, dreams, daydream, lost, evil, supernatural, ghosts, vampires, undead, paper
Med-Heavy RP Guild on Faeblight of Rift, focusing in on underground politics and blackmarket dealings.
#crow, med-heavy, guild, faeblight, rift, focusing, underground, politics, blackmarket, dealings
The Crow Hunting Forum With A Little Extra Punch!
#crow, hunting, foxpro, duck, goose, geese, varmints, saltwater, fishing, freshwater, crowbuster, crowbusters, buster, busters, shotgun
Welcome to Deadman Wonderland! A famous prison where the prisoners are the entertainment, and their destruction your pleasure. Watch freaks of nature fight for their survival with weapons made from their blood, and enjoy the screams of agony they emi
deadman, wonderland, psychopath, prisoner, anime, most, famous, attraction, japan, 'deadman, wonderland'!, tourist?, manga, carnival, corpse, undertaker, #crow, hummingbird, forum
Free forum : A peaceful town with strange things abound.
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The official forum for CBTech, and Crow-Bunny-Studios game development, talk up people!
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Crimson Crow Mod Forum. Crimson Crow - Forum. Crimson Crow Forum
Wednesday 13 Fan Forum
wednesday, bourbon, #crow, frankenstein, drag, queens, murderdolls, rock, metal, horror, glam, shock, gunfire
Fight for your right to burst!
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The original text plus community now has a forum!
ride, maximum, ultimate, roleplay, avian, #crow, bird, james, patterson


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EasyDNA Introduces BritishDNA Ancestry Testing
Chatswood, India – 18/03/16 – EasyDNA introduces its latest ancestry testing service, the BritishDNA test – a test specifically targeted at mapping one's British heritage and aimed at individuals of British descent who want to know more about their British roots.
The launch of the test is in line with the company's mission of expanding its current portfolio of ancestry tests in order to meet the current trends in this field of DNA testing. The demand for ancestry testing services has risen steadily over the years as confirmed by a company representative who stated, "Ancestry testing has become a big market and generated interest across all strata of society. Many individuals want to trace their roots and hone in on their geo-genetic ancestry. People want to find links between their DNA and the geographical regions from which their ancient ancestors originated. The BritishDNA test is not just any generalized ancestry test – it a very niche service which targets a small but specific and well-defined segment of our client demographics".
The latest DNA test provides results which are divided into 7 sections; the fatherline which specifically analyses a person's paternal genetic heritage, the motherline which specifically analyses a person's maternal genetic heritage, a section known as "All my ancestry" which gives a generalized ancestry overview linking the individual's DNA to geographical regions. Also included are 3 other sections which are the baldness test, a DNA test which will indicate your genetic propensity to hair loss, the blue eye genotype test, and the red head test, which will show whether you carry any of the red hair gene variants often associated with Britain. Samples are collected using a simple home sample collection kit requiring just a spit sample from the interested party and results are ready in 8-10 weeks.
On a concluding note, EasyDNA states that "the search for your ancestry is the search for a part of your identity. This is a deep ancestry test that will enable clients to uncover the epic journey of their ancient ancestors from their origins to Britain's s shores. It is a really unprecedented way of discovering your roots, specifically your British roots, giving you information that no traditional amount of ancestry research could reveal".
About EasyDNA
EasyDNA is an international organization at the forefront of DNA testing companies offering the widest range of DNA tests currently available. EasyDNA now operates through an international network of offices in over twenty countries, offering clinical testing, parental testing, relationship testing, ancestry tests and many other tests.


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Your credit score has a huge impact on the homebuying process. You probably already know that it will be a big factor in determining if you qualify for a mortgage loan and what type of interest rate you will be offered. However, you may not be aware that your score can even affect things like how much you will pay in private mortgage insurance.
What is PMI?
When you put down less than a traditional full 20% on your mortgage, it increases the risk of loss to your lender if you were to default on your mortgage. They may not be able to recoup all their investment in your loan after selling and administration costs if there isn't already a significant amount of equity in the home. To compensate for this risk, lenders will require borrowers with down payments of less than 20% to pay private mortgage insurance, or PMI. Even though you pay the premiums, the policy actually protects the lender, giving them a payout to cover their expenses if you should go into foreclosure.
The good news is that once you reach 20% equity in your home, the PMI can be canceled. That can happen by either simply paying down your mortgage balance over time or by increases in your home value due to a robust housing market.
How Does Your Credit Score Affect Your PMI?
Your PMI can cost anywhere from 0.25% to 1.5% annually of the total amount of your mortgage. For example, if you borrowed $300,000 for a home loan, you could pay as little as $750 or as much as $4,500. While the size of your down payment and your debt-to-income ratio factor into how much your premium will be, your credit score is one of the biggest influences on your PMI payment amount. Typically, the higher your credit, the lower your payment will be.
For example, if you have very good credit – a FICO score of 740 or more – your PMI could be in the range of 0.25% to 0.3% of your loan total. On that same $300,000 mortgage from above, that means a monthly payment between roughly $60 and $75.
If your credit is good – a FICO score between 670-739 – you might be offered a PMI rate in the range of 0.35% to 0.40%, translating to a PMI of $90 to $100 a month.
If your credit is only fair – a score between 620-660 – you'll probably be paying the upper range of PMI, somewhere between 0.75% and 1.5%, equating to a monthly payment of $190 to $375.
And this PMI is all in addition to the mortgage principal, interest, homeowners' insurance, and property taxes you are responsible for as a buyer.
Ways to Bring Down Your PMI premium
If you have the resources, putting a larger down payment could decrease your PMI charges, even if you don't put down a full 20%. A down payment of, say, 7% will definitely lower your costs compared to a minimum down payment of 3%.
Since debt-to-income ratios are also important in determining PMI prices, you could try paying down some of your debts to lower that rate.
And of course, improving your credit score is a very impactful way to lower your PMI fees. This generally takes time – somewhere between 6 and 18 months depending on the reason for your low score. If you have fair credit, it may be worth it to hold off on home buying until you can increase it into the "good" range, as you'll pay dramatically less for PMI, not to mention less for your mortgage in general.
If you have any questions about PMI or any other part of the mortgage process, please give us a call today!
These materials are not from HUD or FHA and were not approved by HUD or a government agency. We do not provide tax, legal or accounting advice