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Highway 20, which runs across central British Columbia from Williams Lake to Bella Coola, is one of the province's most beautiful drives.
The big problem with it, from a tourism perspective, is that it's effectively a dead end. Since the provincial government halted the summer-only, direct ferry service from Bella Coola to Port Hardy on Vancouver Island, anyone who drives into the small community on the Central Coast has to drive back out – or face a gruelling 16-hour ferry trip that involves a boat transfer at Bella Bella. The first leg of that BC Ferries journey is on the Nimpkish, a small vessel that has room for only 16 cars. Miss it because it's full and you have to wait up to six days until the next sailing.
Not surprisingly, Central Coast tourism has suffered since BC Ferries cut back on its direct service to Port Hardy in 2014, in the process breaking a key link in what was known as the great circle tour. That route allowed tourists to drive from Vancouver to Bella Coola, returning via Vancouver Island.
On the Highway 20 section of that drive, you drop down into the dramatic rift cut by the Fraser River, just west of Williams Lake, and then cross the thickly forested interior plateau, passing through the small towns of Riske Creek, Chilanko Forks and Kleena Kleene.
By the time you reach Anahim Lake, the rugged Coast Mountains are looming ahead in Tweedsmuir South Provincial Park and then the highway drops with dizzying speed into the Bella Coola Valley.
This is the only road that takes you into the heart of the Great Bear Rainforest.
You can spend two or three days driving from Williams Lake to Bella Coola, stopping along the way in rustic lodges or lakeside log cabins.
In the Bella Coola Valley you can go bear watching, or salmon fishing, or hiking along wild pathways, including the Grease Trail, which has been in use for thousands of years.
The area's first tourist, explorer Sir Alexander Mackenzie, used that well-trodden trail on his epic trip to the West Coast in 1793.
There are also the remarkable petroglyphs just outside Bella Coola that have to rank as one of the world's great archeological sites. And you can jump off here, or from Bella Bella, for boat tours into the Great Bear Rainforest.
Not surprisingly, in a recent report to the provincial government, Aboriginal Tourism B.C. identified the Central Coast as having "immense growth potential" for adventure tourism.
The report cited as attractions the area's stunningly beautiful geography, its rich rainforests and the populations of grizzly and Kermode, or spirit, bears, which have white fur.
"The region also boasts a rich cultural heritage that many First Nations communities wish to revitalize and share," it states. "However, growth in the Central Coast aboriginal tourism sector has lagged behind other regions of B.C., likely due to transportation access challenges."
The report notes that while aboriginal tourism in B.C. overall increased by about 100 per cent in the past five years, on the Central Coast, the growth was only 15 per cent to 20 per cent.
The report said a study has estimated that 11,000 to 18,000 visitor trips annually could be stimulated to the region "through a combination of produce development, marketing, and enhanced access."
By enhanced access, they mean better ferry service.
"For isolated coastal regions such as the Coast-Chilcotin and North [Vancouver] Island, ferry services play the single most important role in facilitating development of the visitor economy," the report concludes.
When the B.C. government cut the direct Port Hardy-Bella Coola service, tourism operators complained that it would hurt their businesses by breaking the great circle tour. And within a summer, they were proven right, as tour buses and recreational vehicle traffic began to dwindle.
The report by Aboriginal Tourism B.C., delivered to the provincial government last week, is an attempt to convince Premier Christy Clark that the Central Coast can be about more than just a place to build LNG facilities.
Bella Coola and Bella Bella sit in landscapes of stunning beauty. The First Nations there want to embrace tourism; they want to show off their rich culture. The Heiltsuk dancers are among the best in the world and Nuxalk cultural guides can take visitors to a petroglyph site, where the ancient faces carved in stone will take your breath away. There are grizzly bears and Kermode bears in the surrounding forests that native guides will gladly take you to see.
Aboriginal Tourism B.C. is asking the provincial government to help them stimulate business by promoting the region – and most importantly, by restoring the direct ferry service between Port Hardy and Bella Coola by next summer.
They have got something wild to sell on the Central Coast. They just need a way to keep the traffic moving.Report Typo/Error
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Apparently, today is No Pants Day
An interactive map of library cats (via Neatorama)
Rapture Index is "The prophetic speedometer of end-time activity." Who knew that false Christs were such a problem?
A friendly reminder that weather is not the same as climate
Friday, May 01, 2009
neat sites
Apparently, today is No Pants Day
Labels: neat sites
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subset: commoncrawl


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I'm 30 y/o and fresh out of a 6.5 year relationship so new to dating. I met this girl this weekend and I notice she likes to text. Is their texting edicate that you guys can help me with? Should I text her for a while before calling and when should I actually call her on the phone? She texted me about getting together this weekend. Any help is great :D
Most Helpful Girl
Call her before this weekend and organise what you two are going to do when you meet up..hearing your voice and tone will reassure her that your interested rather than texting plans through.
Also what do you mean by text edicate? What are you wanting to know?00|00|0
Like are their norms or do things happen a typical way when it comes to texting? Basically she likes to text me but I'm not sure if I should call. I still live with my ex so I like being able to text so my ex can't hear what I'm saying.
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I'd love to try and help. You can always instant message me
Its not letting me IM you. What's your question?


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i mean their just my friends, but should I wear jeans or can I just relax and wear my sweats? is that considered a no-no in eating out, or can I just wear them. if this matters, their black, not gray.
Most Helpful Guy
What would it matter if your friends are black. Eyyyy.
Anyway uh. If you want to do it, 'cos I mean, that's you. Me, I find it unfathomable that people wouldn't wanna dress for dinner! But unless they see it as a really, really big thing, then just dress how you'd like.00|00|1
I meant my sweatpants are black, not my friends LMAOO
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Ahaha well it's not a big thing..it's just me and my 2 guys friends. and I'm not trying to impress them. oh well. I'll figure it out
Lol, you really shouldn't think about it so much like.


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About This Product
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Style: OtterBox Google XL 5.5" Pixel Commuter Case
Get your phone ready to survive the daily grind! The OtterBox Commuter is built for business - and when we say business, we mean business. Dual-layer defense guards against drops and tumbles, while the slim pocket-friendly design makes a serious impression.
- Designed for the Google Pixel XL
- Materials: Synthetic rubber and polycarbonate
- Construction: 2 shock absorbing materials are molded together for the strongest protection in the slimmest case
- Screen bumper: Raised, beveled edge helps protect touchscreen
- Slim profile: Sleek design slips easily into pockets and purses
- Easy installation: One-piece design slips on and off in a flash
- Custom-shaped for perfect fit in an effortless installation
This product is recommended for ages 6+
Will be made by
OtterBox + Zazzle
Fort Collins, CO / Decorated in San Jose, CA
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Sold Out
This crocheted airplane has been lovingly handmade and would make a wonderful addition to any nursery. It is substantial in size for little hands to hold and comes outfitted with a bell inside that jingles carefully when shaken.
A perfect example of Anne-Claire Petit's work. The authenticity, attention to detail, and quality of craftsmanship make each piece unique and unforgettable.
Hand crocheted from 100% organic cotton.
May be washed on a gentle cycle in cold water and air dried.
Anne-Claire Petit studied industrial design and began her career as a designer for Espirit. After four years at the international label, she began her own label under the name anne-claire petit accessories. She first became known for her successful shawl collection and then switched her focus to an extensive children and accessories line, with everything done in a colorful handiwork.
Every Anne-Claire Petit accessory is hand made according to traditional handiwork techniques, using recognizable color schemes and natural materials, which lends to each and every piece being unique. The brand has an optimistic view of the world and this translates into all aspects of the line, from design to production. Each product is cheerful, colorful and made with love, and brings about a positively happy reaction.
Sold Out
More Info. The Brand Stack and create your own creatures with this 14 piece...
$40.00 $50.00
More Info. The Brand This beautifully designed wooden rocket is a puzzle and toy...
More Info. The Brand This beautiful handcrafted oak race...


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I know somewhere on here I saw directions for this chair. I can not locate them now. Can some point me in the right direction..
Can't help, but I'd love to know too.
I think this might be what you are looking for:
That link took me to the main page. Where did you find it?
Go to forums. Do a search for chair template. There is not an actual template, but just a set of directions. The thread title is "Sculpting Aderondak chairs for my cake"
I will attempt to post the link again. Gotta love computers!


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Each month, Rated People connects over 50,000 homeowners with quality tradesmen - simply enter your job details in the form above to get started.
We'll match your job to the relevant tradesmen in your area - those interested will pay to see your contact details and call you to quote.
As each tradesman expresses interest in quoting on your job, we'll send you their profile and ratings to help you choose the right one for you.
After the work is complete, return to the site and rate your tradesman based on their quality of work, reliability and value for money.
Security systems / Alarms
Added 31/07/2017
new and better security lock on front door.
Security systems / Alarms
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we require a full cctv system for our place of worship venue
Searching for tradespeople
Security Specialist
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to fit 4 cctv camaras front side and back
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I have a Scantronic 9449 alarm system which has been going off the past couple of nights for...
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Moving into a 3 bed house in Bletchley soon and need a quote on an alarm system. Hopefully...
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Great job! Will use again
Managing your job is easier with the Rated People Homes App, download it today.


text: I grew up in Des Moines, Iowa and lived there most of my life. I currently reside in Beijing and have lived in China for almost 4 years. As a new phase of my life, I will be moving to Latin America in August 2015. I enjoy movies, reading, and computers. I have circumnavigated the globe. I have been south of the equator twice. I once navigated a 19,000 ton US Navy ship through the Atlantic ocean. I have been to the top of the CN Tower in Toronto Canada. I have lots of experience in teaching English overseas. I enjoy helping people learn new skills.


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It's been said that the world is made up of 4 types of people: 1) Those that don't recognize the problems that exist in our society; 2) Those that recognize the problems, but don't care enough to act; 3) Those that recognize the problems and are too overwhelmed or frustrated to act; 4) Those that recognize society's problems and work actively towards solving them. Which type of person are you?
No one likes to think of themselves as oblivious to the world's problems or worse, too uncaring to try and solve them. When asked what type of person they are, most people see themselves as either number 3 or number 4-too overwhelmed or frustrated to act , or actively doing their part to make positive changes in the world. The truth is that most of us has been all of these "types" of people at point or another.
Sometimes people don't have enough information to recognize that a problem exists. At other times, certain problems don't seem like much of a priority. Most of us try to do our part, whether it's writing a letter to the editor of the local newspaper, voting in a state or national election, or shopping for organic products at our neighborhood grocery store. All of these actions are forms of activism and they all help create positive change.
Many of society's problems seem insurmountable. At one point or another you've probably found yourself throwing up your hands in disgust and asking, "I'm just one person. Things will never change, so why should I even bother?" To quote Mahatma Gandhi, " You may think your actions are meaningless and that they won't help, but that is no excuse, you must still act."
Here are five things you can do to stay passionate about activism:
Don't get caught up trying to solve all of the world's problems. You can't. No body can. Focus your energy where you think you can do the most good. Work on the things you care most about and rest assured that there are people working on the things your not.
At times, activists can feel alone, out of place and misunderstood. Don't despair, you're not alone. Right now there are millions of people out there who have similar concerns as your own. Finding ways to connect with them either locally, or on the Internet can be a great source of inspiration and help you clarify your ideas. Place an ad, start a discussion group or join a message board.
Who are the people making things happen? Find them and find out what their approach is to being a catalyst for change. If you can't find a mentor in the present, look to the past. History is filled with people who worked tirelessly throughout their lives to find solutions to society's problems. Learn about how they did it and become re-inspired .
Understand that change happens slowly and that you may not live to see the "equal rights", the "clean-up" or the "cure" that you're fighting for. That's okay. Neither did Martin Luther King, Jr., Rachel Carson or Christopher Reeves. They got us closer, though, and so will you.
If it seems like everywhere you turn you're bombarded with another headline or news report about the world's problems, it's because you are. Take a news break. Don't worry, the world will keep turning without you. Stop watching, listening or reading the news for a month and you'll be amazed at how much better you feel about the world. When you "turn it on" again, be more diligent about giving your energy away to the sensationalism of it all. Positive change comes faster when people are pro-active rather than re-active.
And above all, know that you ARE making a positive difference in the world. As Margaret Mead once said, "Never doubt that a small group of thoughtful, committed citizens can change the world. Indeed, it is the only thing that ever has."
Looking to get inspired? Here are a few great links to get your started:
About The Author: Ellen Brown is an environmental writer and photographer and the owner of Sustainable Media, an environmental media company that specializes in helping businesses and organizations promote eco-friendly products and services. Contact her on the web at http://www.sustainable-media.com
Add your voice! Click below to comment. ThriftyFun is powered by your wisdom!
Thank You Ellen Brown for the words of wisdom.
stormy from Ca.
Add your voice! Click below to comment. ThriftyFun is powered by your wisdom!


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"Clean, calming and great team"
About: The Royal London Hospital The Royal London Hospital E1 1BB
Posted by Anonymous
I had to take my girlfriend to the A&E dept. late this evening and after a fairly traumatic day the staff here really put our minds at ease.
They were all very friendly even thought there were some rude, drunk and obnoxious people in there (they were sorted out by the hospital security team).
I felt we were dealt with in a timely fashion and they were good at prioritising the most important cases (ours wasn't one of them but we didn't mind waiting a bit).
I have just got home and it's 4am but I felt compelled to write a review giving thanks before bed.
Response from The Royal London Hospital 8 months ago
Thank you for your kind message and we hope your girlfriend is doing well. Best wishes, Barts Health NHS Trust.
- {{helpful}} of {{total()}} people think this response is helpful
- Was this response helpful? Yes | No
Thank you for your feedback.
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Although Evers has two in the boat, he should have had a limit by now. In the last few minutes he's missed a couple more. He's not totally flustered at the moment but the missed bites do bring out a little angst each time. He needs to start getting a hook into a few of them. From experience, this kind of fishing gets better as the day progresses.
Evers is so far back up this creek/river, he's lost all his spectators from this morning. That's a good thing. But in the last few minutes he has gained a couple of spectators. Two young boys are watching, having made it up the creek paddling a canoe. If Evers gets this type of area to himself, with only a couple of paddles churning the water, he could do some damage. He's just got to start getting a hook into some of these missed fish.


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Anchorage, AK After School Programs
Learn about After School Programs businesses in Anchorage, AK. Get the latest local news and offers for After School Programs near you.
Most Recent from Anchorage, AK After School Programs Businesses
Parents know after-school kids activities are a good idea because postsecondary education options often choose students partially based on their extracurricular commitments. But why are these programs so important for kids that colleges ...read more
When the final school bell of the year rings, your kids jump for joy. But, as the days dwindle on, they probably come to you saying they're bored. Skip excessive screen time and explore your city together. Below, Northern ...read more
Anchorage, AK After School Programs Businesses
703 W. Northern Lights Blvd. Suite 200
Anchorage, AK 99503
At Northern Lights Preschool & Child Care kids are given the opportunity to understand and improve their unique learning style. As a comprehensive learning center that provides day and after-school care, their convenient hours a...
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Kids are naturally curious and eager to learn about the world around them. When it comes to early childhood learning, however, some little ones hit hurdles due to issues like fidgeti...read more
Developing study habits at an early age is essential to achieving academic success. While most adults craft their study habits over the years, children need guidance t...read more
Early literacy is about more than just giving your kid a head start in school—though that can be a boon in itself. It not only encompasses teaching your child to read, but many other...read more
The early years of your child's life are extremely influential, building a strong foundation of skills they'll use for many years to come. Because of this, Northern Lights Presc...read more
Your child is going to learn a lot in preschool, but there are a few things they should have learned before getting there, too. From encouraging independence to working on a child's ...read more
Engaging before- & after-school care can transform a child's out-of-school hours into an opportunity for learning and forming friendships. Northern Lights Preschool & Child C...read more
For some children—and parents—the time leading up to the first day of preschool can be a mix of excitement and separation anxiety. This anticipated milestone can be less scary with a...read more
As a parent, you want to make sure that you're making the best decisions to support ample early childhood learning opportunities for your kids. One way of ensuring that your child ha...read more
With summer in full force, you may be wondering how to keep your children occupied over the long school break. Northern Lights Preschool & Child Care, Anchorage, AK's premier lea...read more
As your children grow older, they're going to need as many opportunities as possible to improve their skills and prepare for a lifetime of success in school and beyond. While yo...read more
Northern Lights Preschool & Child Care is an innovative learning center in Anchorage, AK. In addition to providing a safe and fun environment for children to learn valuable socia...read more
As the new school year approaches, many families in the Anchorage, Alaska, area are looking for safe, reliable, and engaging before & after school care for their children. N...read more
Dropping your child off at a daycare center before you go to work might not always be the easiest way to begin your day. You might find yourself worrying about illnesses, physical in...read more
Northern Lights Preschool & Child Care in Anchorage offers a range of age-appropriate programs for children of all ages, from infant care to before- & after-school care for e...read more
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text: 08Apr Private Exchanges May Offer Shelter from Cadillac Tax Categories: Health Care Reform, Team K Blog Tags: ACA, Cadillac tax, exchanges, HIX, PPACA, private exchanges Comments Off on Private Exchanges May Offer Shelter from Cadillac Tax Avoiding, or at least putting off, the so-called Cadillac tax in the Patient Protection and Affordable Care Act is on a lot of employers' minds. One of the best ways to do so is by moving employees to one of the burgeoning number of private insurance exchanges. Read more


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i have been dating this guy for a few months and i want him to meet my mom and sister. This guy is 24 he has no job yet, a daughter from a previous relationship 4 years ago, and lives with his sister, and has a car but its not working and he has to get it fixed but he drives his sister car when she lets him. The reason he does not have a job is becuase him and his sister are going into their own business together (a recording studio). They already have the building and are looking into bying materials, supplies, equipment now. THey are very serious about their business. His sister helps out if he needs money. The only problem is my mom says is a boy is not in college then he should have a job. He should not rely on his sister or anyone for money. If he has a girlfriend he should want to spend money his money on her instead of asking others for money to spend on his girlfriend. My mom doesn't want him taking advantage of me cuz im working and graduating from college. I know my boyfriend is not the type of person to ask me for my money becasue he has not done so yet. I just think my mom is pre judging him before meeting him becuase he's not the typical guy that goes to college, has a job, or nice car. Is my mom wrong for thinking this way? What should i do? I dont want my mom to make my boyfriend feel like he's a bad guy.
Boyfriend meets parents help?
Have an opinion?
What Guys Said 2
Your mother is not 'pre' judging him, she's only calling out what she sees in front of her. Step back away from your feelings and think of it logically - a 24 year old who already had a kid and has split from the mother, no higher education, no job and leeches off his sister - what conclusion would you draw?00|00|0
Let me guess. He's black and you're white.00|00|0
umm that rude of you to even think like that IF we were those races.
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I can see your against interracial couples. im guessing our a white dude and your girl friend left you for a black guy. sucks doesn't it.
I'm not against interracial couples. Whites, Asians, hispanics, indians, etc. I just think white girls who sleep with black guys are trashy. I've never been cheated on, or have been left for a black guy. Good guess though.
What Girls Said 1
Your mom has a point. However, she might like him more once she meets him and sees how well he treats you :)00|00|0
He treats me respectfully. i just want her to see what i see in him. he doesn't take advantage of me.
Learn more
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Frugal Spending, Big Rewards — Alton's After-Showby Maria Russo in Shows, September 22nd, 2013
It's no surprise that to be successful on Cutthroat Kitchen competitors ought to come equipped with a strategy for how they'll approach the contest, as Alton's culinary mind game requires more of contestants than basic kitchen chops and the ability to work under pressure. For a chef to be victorious, he or she will need a strategy, and this week's champion ultimately claimed the win thanks in part to a method of restrained bidding. After three rounds and only two wins at the auction, the top chef left with $11,800, a grand sum compared to the small wages some rivals have taken home. Alton and judge Jet Tila dished on such an approach to the contest during the latest installment of the host's After-Show. "You want to walk out of here with your dough," Alton explained. Jet added, "You're not here just to spend, spend, spend to sabotage people." On several past episodes, chefs have gotten caught up in back-and-forth bidding wars only to "spend their way to victory," as Alton noted. This week's victor, however, claimed just two wins at the auction, guaranteeing a take-home sum of $11,800, a large figure compared to the small wages some rivals earn after three rounds of seemingly careless spending. What did you think of the spend-less-to-win-more strategy? Is it a riskier maneuver, given that a contestant may find himself saddled with sabotages if he isn't the one doling them out? Click the play button on the video above to hear more from Alton and Jet, and learn which chef claimed victory, plus more about how he or she managed to secure the win. Then start the conversation with fellow fans in the comments section below. Tune in to a new episode of Cutthroat Kitchen on Sunday, Sept. 29 at 10pm/9c.
More posts from Maria Russo.
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Potential roles of microRNAs in regulating long intergenic noncoding RNAs
Proceedings of the 2011 International Conference on Bioinformatics and Computational Biology (BIOCOMP'11)
The 2011 International Conference on Bioinformatics and Computational Biology (BIOCOMP'11)
Over 10,000 long intergenic non-coding RNAs (lincRNAs) have been identified in the human genome. Some have been well characterized and known to participate in various stages of gene regulation. In the post-transcriptional process, another class of well-known small non-coding RNA, or microRNA (miRNA), is very active in inhibiting mRNA. Though similar features between mRNA and lincRNA have been revealed in several recent studies, and a few isolated miRNA-lincRNA relationships have been observed. Despite these advances, the comprehensive miRNA regulation pattern of lincRNA has not been clarified.
In this study, we investigated the possible interaction between the two classes of non-coding RNAs. Instead of using the existing long non-coding database, we employed an
Approximately 90 lincRNAs show strong reverse expression correlation with miRNAs, which have at least one predicted target site presented. These target sites are statistically more conserved than their neighboring genetic regions and other predicted target sites. Several miRNAs that target to these lincRNAs are known to play an essential role in breast cancer.
Similar to inhibiting mRNAs, miRNAs show potential in promoting the degeneration of lincRNAs. Breast-cancer-related miRNAs may influence their target lincRNAs resulting in differential expression in normal and malignant breast tissues. This implies the miRNA regulation of lincRNAs may be involved in the regulatory process in tumor cells.
Deep sequencing data from the Encyclopedia of DNA Elements Consortium (ENCODE) suggests that 70-80% of the human genome can be transcribed, and non-protein-coding RNAs (ncRNA) exceed the number of protein-coding genes
In two recent studies, over 10,000 lincRNA regions were identified in human and mouse genomes. These regions were discovered based on epigenetic marks, where the promoter mark H3K4me3 is followed by the transcript mark H3K36me3. Comparative genomics analysis suggests that a significant portion of these regions do not have strong protein coding potentials, and do not have sequence homology with other known proteins
MicroRNA (miRNA) is another type of noncoding RNA whose biological functions have been extensively studied. MiRNAs are ~22nt small non-coding RNAs
Despite the extensive investigation on the roles of miRNAs in regulating protein-coding genes, only a few isolated non-coding transcripts (CDR1 antisense and MEG3) are reported to be regulated by miRNA
GENCODE project and NONCODE database have both collected and integrated long noncoding RNA annotations
RNA-seq experiments sequence millions to billions of short RNA fragments in a single experiment, by which it measures the expression levels of the whole transcriptome, including noncoding RNAs, without relying on detailed annotation. To explore the potential miRNA-lincRNA expression relationship, we analyzed RNA-seq data from a group of normal and malignant breast tissues. Random primers were used in the reverse transcription step, and the strands of the transcripts were preserved to aid in identifying non-coding RNAs that are not poly-adenylated. Due to their small size (~22nt), the mature miRNAs were not included in the sequencing libraries. However, our data suggested that we can detect precursor miRNAs (80-120nt) from these RNA-seq data sets. For lincRNAs, the sequence fragments across two exons of the transcripts (junction reads) are able to reveal exon boundaries, especially novel ones. This feature helps us to understand the splicing patterns of unannotated lincRNAs. Several tools have been developed for mapping junction reads
In this study, for each putative miRNA-lincRNA pair, (1) we required reverse correlation between the expression levels of pre-miRNA and lincRNA within the 20 examined tissue samples. This is due to the well-established roles of miRNAs in facilitating RNA degradation on protein coding genes; and (2) we also required seed sequence of the miRNA (positions 2-7 on the miRNA) should be present in the exon regions of identified lincRNA, and the surrounding sequences on the lincRNA should also favor miRNA targeting conditions. We further examined the evolutionary conservation of the identified miRNA seeds in lincRNAs.
Overall strategy
In order to investigate the potential relationships between miRNA and lincRNA, we used RNA-seq data from 20 samples, including 10 breast tumor samples, and 10 breast tissues from healthy pre-menopausal volunteers with no history of disease; these normal tissues were obtained from the Susan G. Komen for the Cure® Tissue Bank at the IU Simon Cancer Center. As shown in Figure
Figure 1
The schematics of the overall study
The schematics of the overall study.
LincRNA annotation
Based on the RNA-seq data, the discovery of lincRNAs in breast tissue includes the following steps: (1) Epigenetic marks and comparative genetics. As reported previously, thousands of lincRNAs have been discovered by searching for the genomic regions with enhanced promoter marks (H3K4me3, or K4) following the transcript marks (H3K36me3, or K36). Our analysis started from 2,513 intergenic K4-K36 regions in the human genome (hg18) and 1,665 intergenic K4-K36 regions in the mouse genome (mm9) from previous studies
(2) Evidence of transcriptional activity in other breast cancer cell lines. We have previously reported genome-wide binding patterns of RNA polymerase II (RPolII) in MCF7 cell lines
Figure 2
RPolII signal, CSF scores, expression levels and conservation in lincRNA regions
RPolII signal, CSF scores, expression levels and conservation in lincRNA regions. (a) The RNA polymerase II ChIP-seq signals in protein coding genes, lincRNA regions, and randomly selected regions. The RPolII signal is demonstrated as reads per kilobase exon model per million mappable reads. (b) The CSF score of the lincRNA regions, protein-coding genes, and randomly selected regions. (c) The RPKM values for the exonic and intronic regions for both lincRNAs and protein-coding genes. (d) Conservation levels for exonic regions, intronic regions for both lincRNAs and protein-coding genes. X axis denotes the conservation score
(3) Coding potential. We evaluated coding potentials of the identified lincRNA regions using the methods presented in
We used Scripture
Additional file 1
Genomic coordinates of 525 lincRNAs showed expression evidence in breast tissues.
Click here for file
Target prediction
We employed TargetScan
Expression reverse correlation
We quantified the expression levels of mRNA, lincRNA and precursor miRNAs using RPKM measures (reads per kilo-base exon model per million mappable reads). In total, there are 15,381 genes, 303 lincRNAs and 286 miRNAs expressed more than 0.5 RPKM in more than 15 samples. Our further analysis focuses on these sets of genes whose expression levels are detectable.
A generalized linear model (GLM) was used for modeling the potential effects of miRNA in down-regulating the expression levels of genes and lincRNAs in tumor and normal breast samples. Among the expressed mRNA, lincRNAs and miRNAs, 38,828 pairs are predicted to have regulatory relationships by miRNA target prediction algorithms. Among these potential target pairs, 1,742 and 213 mRNA-miRNA and lincRNA-miRNA pairs also showed significant reverse correlation, as derived from the GLM model (FDR<0.2).
Among the 213 lincRNA-miRNA pairs, there are 315 predicted miRNA target sites (Additional file
Additional file 2
Genomic coordinates of the 315 predicted microRNA binding sites on lincRNAs.
Click here for file
Figure 3
Conservation score of predicted binding sites of miRNA binding sites on the lincRNAs
Conservation score of predicted binding sites of miRNA binding sites on the lincRNAs. X axis denotes
We further examined whether miRNAs showed difference in expression relationship with their potential target genes/lincRNAs in tumor and normal tissues. Of the predicted gene/lincRNA-miRNA pairs, 814 (~42%) show differences in expression relationships with a significant p-value for the GLM interaction factor (p < 0.05), including 121 (~57%) lincRNA-miRNA pairs (one example is shown in Figure
Figure 4
MiRNA and lincRNA expression show different relationship between cancer and normal tissues
MiRNA and lincRNA expression show different relationship between cancer and normal tissues. X axis denotes miRNA expression. Y axis denotes lincRNA expression. Blue and red dots are for normal and tumor samples, respectively. (a) Hsa-miR-148b down-regulate linc104 in tumor samples, but not in normal samples. The coefficient of interaction item
As a class of newly discovered non-coding RNA, genomic loci of lincRNAs are not well annotated. As an
Several recent studies revealed that thousands of lincRNAs exist in the cell
In addition to the data from RNA-seq experiments, two factors were considered for lincRNA identification, the transcriptional activity, and coding potential. ChIP-seq signals of RNA polymerase II (RPolII) were used to quantify transcription activity. One potential drawback of this strategy is that the RPolII data is from MCF7 cells due to the lack of measurement for the breast and tumor samples. This may cause bias in estimation. To ensure the RPolII data is representative of the transcript activity in breast, we surveyed the correlation between RPolII signal of MCF7 cell line and the RNA-seq levels of the breast tissues. We observed a very strong correlation (p < 6.8e-186, Spearman correlation) between RPolII signal in MCF7 cells and RNA-seq data from normal breast samples (Figure
Figure 5
Correlation between RPolII ChIP-seq signals in MCF7 cells and RNA-seq expression in breast tissues
Correlation between RPolII ChIP-seq signals in MCF7 cells and RNA-seq expression in breast tissues. X axis denotes RPolII ChIP-seq signal level. Y axis denotes RNA-seq expression. (a) RNA-seq from normal breast tissue. (b) RNA-seq from breast tumor.
MiRNAs regulate degradation of its target mRNA through binding on the 3'-un-translated regions. Since the entire noncoding RNA is un-translated, the whole mature transcript can serve as potential miRNA target sites. We therefore predicted miRNA binding sites on the entire exonic regions of the identified lincRNAs.
One advantage of RNA-seq data is that it can simultaneously measure the expression level of both mRNAs/lincRNAs and precursor miRNAs in the same sample. This provides opportunity for revealing the relationship of the mRNA/lincRNA-miRNA in their expression levels. As a precursor stage of mature miRNA, pre-miRNA expression levels can be different from their final functional form. Therefore, major conclusions from this analysis are subject to further experimental validation.
Among 30,069 mRNA-miRNA pairs whose relationships were established based on target prediction alone, 31 pairs were experimentally validated, based on the records documented in the miR2Disease database and TarBase database
RNA-seq data analysis
cDNA libraries from 10 normal breast tissues from the Susan G. Komen Tissue Bank at the IU Simon Cancer Center and 10 triple-negative breast cancer tumors were sequenced on an Applied Biosystems (ABI) SOLiD3 sequencer. We used a customized pipeline for RNA-seq data analysis, which includes three steps, QC filtering, sequence alignment, and gene expression quantification. 1) QC filtering. We first used SOLiD™ Instrument Control Software (ICS) and SOLiD™ Experiment Tracking System (SETS) software for the read quality recalibration. Sequences containing more than two 'N' or wildcards were discarded. Each sequence was scanned for low quality regions, and if a 5 base sliding window had an average quality score less than 20, the read would be truncated at that position. Any read with a length of less than 35 bases was discarded. Our experience suggests that such strategy effectively eliminates low quality reads while retaining high quality regions. 2) Alignment. We used BFAST
In potential lincRNA regions, we systematically searched regions with short read enrichment as putative lincRNA exons. A putative exon must contain at least 8 reads. If the distance between two exons was less than 10 bases, the two exons would be merged together. To define the exact exon boundaries, we searched for potential splicing sites around the putative exons. Splicing donor/acceptor (GT-AG or CT-AC for RNAs on Watson or Crick strand respectively) sites within 25 bases from the 5'- and 3'- ends of two putative exons would be considered as possible splicing sites. A lincRNA junction library was constructed by connecting all the possible donor and acceptor sites within the 100,000 bases span. The minimum intronic length is set to be 70 bases. After aligning the remaining unmapped reads to the new lincRNA junction library, we used Scripture
Conservation, protein coding potential, and miRNA target prediction
We calculated the conservation score across placental mammals using Siphy
We used the Codon Substantial Frequency (CSF) score to measure the coding potential of candidate lincRNA regions
The mature and precursor miRNA annotations were extracted from miRBase
Generalized linear model, Wilcoxon test, and false discovery rate
We used a generalized linear model (GLM) to characterize the relationship between the expression levels of genes, lincRNAs and miRNAs, by treating gene/lincRNA and microRNA as dependent and independent variables, respectively. Assuming the number of the reads falling into a genomic region follows a Poisson distribution, a natural logarithm function was chosen as the link function:
In which
To allow over-dispersion, in practice, we employed a quasi-
To correct for multiple hypothesis testing, false discovery rate (FDR) was calculated based on Benjamini-Hochberg correction
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
LJ, YW, and YL contributed to the design of the study. LJ and YL designed and performed the computational modeling and drafted the manuscript. GW, SC and YW participated in coordination, discussions related to result interpretation and revision of the manuscript. MR, BS and SC contributed to the experiment performing and data collection. All the authors read and approved the final manuscript.
This work was supported by grant from the National Natural Science Foundation of China (60901075, 60973078), the Natural Science Foundation of Heilongjiang Province of China (LC2009C35), the Special Research Foundation for technological Innovation Talents of Harbin (2011RFLXG011), and funds from the United States National Cancer Institute grants CA113001.
This article has been published as part of
The ENCODE (ENCyclopedia Of DNA Elements) Project
Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals
Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression
A large intergenic noncoding RNA induced by p53 mediates global gene repression in the p53 response
The Igf2/H19 imprinting control region exhibits sequence-specific and cell-type-dependent DNA methylation-mediated repression
Genome-wide computational identification and manual annotation of human long noncoding RNA genes
Annotating non-coding regions of the genome
New families of human regulatory RNA structures identified by comparative analysis of vertebrate genomes
Noncoding RNAs: the missing "linc" in p53-mediated repression
Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis
Long noncoding RNA as modular scaffold of histone modification complexes
Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs
Noncoding RNA in development
Genome-wide analysis of long noncoding RNA stability
No-nonsense functions for long noncoding RNAs
lincRNAs act in the circuitry controlling pluripotency and differentiation
Polycomb proteins targeted by a short repeat RNA to the mouse X chromosome
The Air noncoding RNA epigenetically silences transcription by targeting G9a to chromatin
A long ncRNA links copy number variation to a polycomb/trithorax epigenetic switch in FSHD muscular dystrophy
The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation
Structural architecture of the human long non-coding RNA, steroid receptor RNA activator
Large non-coding RNAs: missing links in cancer?
Emerging functional and mechanistic paradigms of mammalian long non-coding RNAs
MicroRNAs: genomics, biogenesis, mechanism, and function
Sustained expression of microRNA-155 in hematopoietic stem cells causes a myeloproliferative disorder
The microRNA Registry
bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila
A microRNA array reveals extensive regulation of microRNAs during brain development
Systematic discovery of regulatory motifs in human promoters and 3' UTRs by comparison of several mammals
MicroRNA expression in zebrafish embryonic development
miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA
microRNA-29 can regulate expression of the long non-coding RNA gene MEG3 in hepatocellular cancer
Mammalian microRNAs predominantly act to decrease target mRNA levels
Argonaute HITS-CLIP decodes microRNA-mRNA interaction maps
Systematic identification of mRNAs recruited to argonaute 2 by specific microRNAs and corresponding changes in transcript abundance
GENCODE: producing a reference annotation for ENCODE
NONCODE v3.0: integrative annotation of long noncoding RNAs
miRcode: a map of putative microRNA target sites in the long non-coding transcriptome
Transcriptome sequencing across a prostate cancer cohort identifies PCAT-1, an unannotated lincRNA implicated in disease progression
TopHat: discovering splice junctions with RNA-Seq
Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation
Ab initio reconstruction of cell type-specific transcriptomes in mouse reveals the conserved multi-exonic structure of lincRNAs
BFAST: an alignment tool for large scale genome resequencing
The human genome browser at UCSC
RNA polymerase II binding patterns reveal genomic regions involved in microRNA gene regulation
Identifiability of isoform deconvolution from junction arrays and RNA-Seq
Statistical inferences for isoform expression in RNA-Seq
RNA-Seq gene expression estimation with read mapping uncertainty
Revisiting the protein-coding gene catalog of Drosophila melanogaster using 12 fly genomes
Performance and scalability of discriminative metrics for comparative gene identification in 12 Drosophila genomes
Most mammalian mRNAs are conserved targets of microRNAs
The role of site accessibility in microRNA target recognition
Long intergenic noncoding RNAs: new links in cancer progression
miR2Disease: a manually curated database for microRNA deregulation in human disease
The database of experimentally supported targets: a functional update of TarBase
MicroRNAs 221 and 222 bypass quiescence and compromise cell survival
Widespread changes in protein synthesis induced by microRNAs
MicroRNA 29c is down-regulated in nasopharyngeal carcinomas, up-regulating mRNAs encoding extracellular matrix proteins
Reticuloendotheliosis virus strain T induces miR-155, which targets JARID2 and promotes cell survival
MicroRNA-17-92 down-regulates expression of distinct targets in different B-cell lymphoma subtypes
SOLiD™ Sequencing and 2-Base Encoding
Identifying novel constrained elements by exploiting biased substitution patterns
Distinguishing protein-coding and noncoding genes in the human genome
miRBase: integrating microRNA annotation and deep-sequencing data
Controlling the false discovery rate: a practical and powerful approach to multiple testing


text:
Today, I attended a cake decorating competition for High School and Culinary School students here in Chicago.
While watching a demonstration from one of the instructors, I noticed that none other than Nicholas Lodge was one of the judges. I could see him and the other judges examining the cakes. He carefully examined each entry and took a lot of time with each one. He took it as seriously as if he was judging a national competition, not some small local event.
I was so impressed with his professionalism and think it is a real reflection of how committed he is to his art. Im signed up for his hands on demo at ICES this summer and now I really cant wait.
Just thought Id share.
wow...how lucky were you!
he seems to be very professional...in his creations, classes, and judging.
He is a wonderful person and instructor!! I have taken several of his classes in Atlanta and what an experience!!! He can teach anyone to work with gumpaste and fondant!! I wish the food network would replace Kerry with him. He is so kind when he judges!!
He is wonderful to work with. I was lucky enough to take a 2 1/2 day class with him. I made a mistake on one of my roses with the petal overlapping the opposite way. I didn't even notice it, but he did when he walked by. He had me fix it and told me he has a trained eye. He's so charming and I just love his laugh too. I would jump on a chance to take another class with him.
I agree - he is amazing. He is an enormously talented artist and a genuinely nice person. His classes are organized and he is focused. His classes are soooooo worth every penny .
I love me some Nicholas Lodge!!!
I was fortunate enough to purchase one of his Sugarcraft books for beginners, here at a discount book store for a very cheap price.
It has a wide range of information, with all sorts of ideas and templates.
which will help me tremendously.
Ican just imagine him cos I so love his books especially the International Sugar Craft book 1-3.I get so much inspiration from his works that I know I would love to meet him and attend one of his courses someday!


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Samsung has already shown off its Tizen based televisions in length. And just as it begins to start shipping TVs running the OS, the company has now announced that it plans to sell close to 30 million units of these televisions before the end of the year. That's pretty ambitious even for a company like Samsung, but considering the dominance it has on the global television market, it's not unachievable.
The company has mentioned that a majority of its 2015 Smart TVs will be running Tizen OS by default, so most televisions you buy from the company this year will have the OS. Samsung is pushing Tizen forward with an aggressive approach towards the market.
The company already has a camera running on Tizen while its entire Gear smartwatch lineup (with the exception of the Gear Live) are running Tizen out of the box. There's even a smartphone called the Z1, but it's only available in some regions at this point.
Source: Tizen Experts
Via: Sam Mobile


text:
Happy Birthday :)
happy birthday :D
Hi mom
Happy birthdayyyyy
Happy Birthday unni :D
Hi Ms. Choi I really admire your acting after I watch Stairway to Heaven and Temptation I really really like the way you and KSW act together the feeling and the prospective action reflect all in one place I really admire you both and hope more drama of you and KSW. May the good Lord bless you. I'm your fan all the way from the South Pacific Island.
Happy Birthday
Happy Birthday!!!
Happy birthday
happy 40th birthday unni!!!!! :D
Happy birthday!!
I think shes overrated. I have seen her in 4 dramas and same frozen expression in each one.
That's because of plastic surgery
The more the years pass, the more beautiful she becomes.
*cough cough* the more plastic surgery she gets....


text:
Isovera is always looking for talented individuals to join our staff, and to assist with the awesome work we perform for world-class clients such as Hunter Boots, Bidsquare.com, and Harvard University.
If you are passionate about what you do, are unable to settle for anything less than quality work, and enjoy a team-focused workplace that will foster and build you skills, Isovera might just feel like home.
To apply to any of the listings below, please write a casual letter letting us know what your skills include, why you feel you might be a good fit for the position, and also include a little about what you enjoy doing in your free time. Please include a copy of your resume with this letter, and send it to Stephen Sanzo at firstname.lastname@example.com.
Become an Isovera-approved Freelancer!
Isovera is always looking to bring in top-notch freelancers to help us deliver award-winning projects.Our Isovera-approved Freelance Team consists of quality experts that serve as an extension of our full-time staff. Apply here.


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ITNP hires head of documentaries
Bafta award-winning producer Guy Davies has joined ITN Productions (ITNP) in the newly created role of head of documentaries.
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Question: Explain the matching process
Explain the matching process.
View Solution:
View Solution:
Answer to relevant QuestionsSupply the missing dollar amounts for the 2014 statement of earnings of Ultimate Style Company for each of the following independent cases: Rowland & Sons Air Transport Service Inc. has been in operation for three years. The following transactions occurred in February: February + Paid $ 1,900 for rent of hangar space in February. Purchased fuel costing $ 450 on ...Internet Marketing Inc. (IMI), which has been operating for three years, provides marketing consulting services worldwide for online companies. You are a financial analyst assigned to report on the effectiveness of IMI's ...Refer to the transactions in E3– 8 (including the example), and prepare a statement of earnings for Peter's Bowling Inc. for the month of October 2014. Use an income tax rate of 40 percent. In exercise Refer to P3– 8, and use the following chart to identify whether each transaction in P3– 8 results in a cash flow effect from operating (O), investing (I), or financing (F) activities, and indicate the direction and ...
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text:
Flamini sad at Emirates events
Mathieu Flamini has revealed his deep concern over the recent turbulent times for Arsenal.
Last Updated: 28/11/08 5:49pm
Mathieu Flamini has revealed his deep concern over the recent turbulent times at Arsenal.
The French midfielder spent four years as a Gunner before joining AC Milan on a free transfer in the summer.
But the 24-year-old admits he is 'still a red' in his heart and he is upset by what has happened at the Emirates Stadium in recent weeks.
The Gunners have got off to an indifferent start to the campaign - losing five games to sit in fifth in the league.
And William Gallas has been stripped of the captaincy after criticising his team-mates in public, with Cesc Fabregas taking over the mantel.
Flamini said: "I am not very happy when I see what is going on with the Arsenal team.
"I'm still an Arsenal fan because I played there for four years. I am still red in my heart.
"The most important thing is to have unity and to have a good spirit to keep going. Last year the Arsenal spirit was amazing. From the back to the front everybody fought for each other all over the pitch.
"I played in midfield with Cesc Fabregas, Alex Hleb and Tomas Rosicky and we were always talking and helping each other. When you fight for each other you win games.
"Arsene Wenger is very important and it is very important to trust him because he has achieved great things at the club but although the manager is always a big influence in the dressing room it is up to the players to get together and work hard for each other.
"When you play in England the most important thing is to win the battle. It is never easy to play in the Premier League because it is physical and every team has strong players."
He added: "I think Gallas said what he did because he was trying to get a reaction from his team-mates.
Never easy
"It is never easy because in this situation you do not know what to do.
"I have not spoken with William in the last few days so I cannot say if he will leave but is it not going to be easy for him to stay.
"In this situation I do not know if I would want to stay but I believe William did not set out to cause problems and that he loved being captain.
"I spoke with Cesc and he is very happy to be given the captaincy. This is very important for him and he feels he is ready to take on the extra responsibility.
"I'm sure he will continue to play as well as he always has and his game may even improve."


text:
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text:
Bocca a Lavu rankings
Difficulty ranking world: 6729 (all)
Ranking France: 1621 (all)
Difficulty score: 40.32 what?
Bocca a Lavu ratings
(rate it)
The Bocca a Lavu is situated in Corsica.
Starting from Pont de Chiuni,
the Bocca a Lavu ascent is 10.6 km long. Over this distance, you climb 471 heightmeters.
The average percentage thus is 4.4 %.
Look for other sides to climb the Bocca a Lavu.
profile Bocca a Lavu - click to open in new window
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text:
24-1 Hoolei 24-1 (T1),
Kihei, Hawaii HI 96753
$2,385,000 Price
3 Bedrooms
3 Total Baths
3,076 Square Feet
30.167 Acres
374045 Listing ID
Schedule Showing Virtual Tour
Status ACTIVE
County Maui
Year Built 2008
Property Type Condos
Property Sub Type Condo
Primary Features
County: Maui
Property Type: Condos
Property Sub Type: Condo
Year Built: 2008
Zoning: 2
Building Name: Hoolei
Status Category: Active
Unit: 24-1 (T1)
External Features
Carport: n
Garage: n
Land SqFt: 1314075
Parking: 119
Photo-Voltaic Installed: n
Pool: y
Waterfront: Across Street from Ocean
Location Information
Complex Name: Hoolei
Condominium Property Regime: 0115
District: Wailea/Makena
Plat: 008
Street Designation: Cir
View: Garden View
Financial Details
Land Tenure: Fee Simple
Monthly Maintenance Fee: 1817.8
Potential Short Sale: n
REO: n
Tax Map Key: 2210081190115
Interior Features
Floor Level: 2
Pets Allowed: n
Zoning Info
This listing is represented by RE/MAX Lifestyle©-2017, REALTORS® Association of Maui, Inc. All Rights Reserved. This information is believed to be accurate. It has been provided by sources other than the Realtors Assoc. of Maui and should not be relied upon without independent verification. You should conduct your own investigation and consult with appropriate professionals to determine the accuracy of the information provided and to answer any questions concerning the property and structures located thereon. Featured properties may or may not be listed by the office/agent presenting this brochure.
Contact - Listing ID 374045
Steven Nickens
3750 Wailea Alanui Suite B34
Wailea, HI 96753
Phone: 8082802208
Phone Alt: 8082802208
Data services provided by IDX Broker


text:
The Koku Well Spent was a group of merchants from the Trading Grounds of Otosan Uchi devoted to the welfare of the Imperial City and its inhabitants. The buildings in the Karada district where they met were known by the same name. [1]
- ↑ Otosan Uchi:Book 1, p. 80
|This Imperial Families related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


text:
Hey, I am looking at doing a 3 - 4 week tour of South America, consisting of Peru, Bolivia and maybe Brazil.
I have found travel companys such as; Tucan Travel, Kumuka and GAP, but I was wondering if there are cheaper
'budget' companies that specialise in South American tours only. I have tried searching through Google, but most of the ones I see are way more expensive than the companies I listed above.
So my question is; are Tucan, Kumuka and GAP the best and the CHEAPEST companies to do a South American tour like this, or are there cheaper companies out there?


text:
By Monique Fields
TUSCALOOSA, Ala (Reuters) - Emeel Salem Jr. cried as he drove through Tuscaloosa on Saturday for the first time since the April 27 tornado ravaged the Alabama town.
"I'm missing turns because the landmarks that used to be there aren't there," said Salem, a University of Alabama alumnus who plays minor league baseball for the Tampa Bay Rays organization.
The Forest Lake home he rented during the off-season was gone. Where the home once stood, he could see Druid City Hospital, a view he didn't have before the storm.
"I knew it would hit me hard," he said. "I didn't realize I'd be overcome with emotions."
Salem said he wasn't worried about himself, but about fellow residents who lost everything.
It's a common response known as secondary traumatic stress or compassion fatigue, said Karla D. Carmichael, an assistant professor of counselor education at the University of Alabama.
Many of the state's residents are dealing with similar emotions in the storms' wake, and mental health experts are taking action to help.
The Alabama Department of Mental Health and the Federal Emergency Management Agency have partnered to activate Project Rebound, a federally funded program agencies use to assist residents when a natural disaster occurs.
Teams of counselors are on the ground helping residents in hard-hit areas, and the state will be able to hire more counselors for community outreach and educational services with FEMA's help, said John Ziegler, director of the public information office for the state's mental health department.
The state will open a call center to provide free crisis counseling as soon as counselors are hired and trained, he said.
"It's later, after the emergency crisis, that people begin to feel the weight of their emotions, and their loss and their grief," Ziegler said.
Roughly two-thirds of Alabama was ravaged by tornadoes on April 27. More than 230 residents died, including 43 in Tuscaloosa alone.
Though many of Tuscaloosa's 95,000 residents didn't lose a home or a relative, they have seen the devastation and felt the loss of neighborhoods, businesses and their sense of security.
Residents can no longer purchase craft supplies at Hobby Lobby, shop for discounted items at Big Lots, have a burger at Milo's or pick up coffee at Krispy Kreme Doughnuts. All those businesses were destroyed in the storm.
"Everybody in Tuscaloosa potentially has secondary traumatic stress to some degree or another," Carmichael said. "There's going to be an increase in substance abuse, compulsive behavior, over-spending, over-eating, gambling, addiction."
Children are not immune from the destruction.
The city's Chuck E. Cheese's restaurant was leveled during the storm. In the days following the tornado, Carmichael talked with kindergartners and first-graders who knew the Chuck E. Cheese character lived there and wanted to know where he had gone and whether he was okay.
"Chuck E. was taken care of just like everybody else," Carmichael told the schoolchildren. "Chuck E. has a new home. He is staying with friends until they build Chuck E. Cheese's back."
Alabamians who survived the tornadoes may experience depression, guilt, difficulty sleeping, fatigue, nightmares or extra worry in the wake of the disaster, experts said.
The Family Counseling Service in Tuscaloosa has been offering free counseling since the tornado and is now seeing residents come in for treatment.
At first, they feel shock and disbelief, followed by anger and resentment. There will be a sense of loss of what used to be before reaching the stage of acceptance, said Larry Deavers, the center's executive director.
There will be progression and regression as the city recovers, and it is normal for residents to experience different degrees of those stages, he said.
"It doesn't mean you can wrap a bow around it and be done with it," Deavers said. "That's just the way humans are developed and the way we process loss."
"The intensity of those emotions right now is temporary. The key is to allow one another to talk or not to talk."
(Editing by Colleen Jenkins and Jerry Norton)


text:
As always: Ellooooo. xP
Okay since I'm such a freak I finally got to adopt........
DRUM ROOOOOOLL............... *creates imaginary drumroll cause no one cares*
The Evanescence wiki.
Okay the only problem now iiiiis that the wiki barely has any pages and I'm the only admin. xP
So what we need now is some contributors, active users and, like, two admins who help out. xD
So, I found out through chat (or well I convinced many many many peeps xD Lalalalala) pretty much a lot of people on here like Evanescence.
So I'm inviting you over there, especially if you like the band to create some pages, edit existing pages and stuff, it needs a whole lotta work.
Comments, badges, chat and all that chizz is turned on, I put on an acceptable layout, so all that's missing is PEOPLE. So pweaseee, if you share a little like/love for Evanescence how bout checking it out? xD
Tah link:
Thanks if you check it out. :P


text:
TRAILER: Devil May Cry Movie To Be Produced In Ghana
If you heed our disclaimer, then you may be in for a shock.
It is, as advertised, a trailer for a cinematic adaptation of Devil May Cry, so we're not lying to you in that respect.
But there are a few caveats that we must mention. Firstly, the production is being made in Ghana, West Africa, most probably in the capital city of Accra. Not that you should feel cheapened by the experience because Ghana's movie sector, much like Nigeria's, is no doubt upwardly mobile and thriving. But you can expect it to differ a little from your standard Western European cultural norms.
Secondly, the effects in this trailer are somewhat… let's just say we're not expecting Pixar-like quality from this project.
What you do get is some pretty awesome voice over work and an attempt at adapting a beloved Capcom franchise for the big screen. Without further ado, you can see the clip for yourself below:
Props: CVG


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Though rain threatened, it turned out to be a gorgeous fall day for Alexa & Tom's Cobblestone Farm wedding in Ann Arbor. Check out some of our favorite images from the day below!
TagsAnn Arbor AnnArbor.com Ann Arbor Photographer Ann Arbor Wedding Anniversary Art Artini Martini Crawl Beautiful Brighton High School Child Cobblestone Farm Detroit DIA downtown DYC Elle Magazine Emily Kay Photography Emily Tishhouse Engagement fun Gallup Park Green happy couple High School High School Senior in love Kalamazoo Maternity Michigan Michigan Wedding Photographer Nature party perfect fall day Photography Resort Salon Vox Senior Trash the Dress TTD University of Michigan urban Waldenwoods Wedding Wedding Dress WMU


text: The gods of fashion have spoken: André Leon Talley, the former Vogue editor-at-large more recently recognized for his position on the judging panel of "America's Next Top Model," will be receiving his very own reality show on Bravo. Finally! ALT, without a doubt one of the most endearingly excellent personalities in fashion, will be the… READ MORE »


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Happy Birthday, Avatar Wiki!
It's hard to believe it's already been a full year since the Avatar Wiki was created. At first, I created the wiki just for fun, and I never imagined that it would reach such an amazing level of quality. After I saw the movie and experienced it, I had the hopes that it would attract hundreds of avid Avatar fans and they can hang out and discuss anything and everything related to James Cameron's masterpiece, and I think we've definitely succeeded in that regard. We would not have survived an entire year without each and every one of you guys that have made an immense amount of edits to the largest resource for free Avatar information and news. I'm honored to share this community with all of you hard workers, and I think the wiki has a very bright future ahead of it. I'm not sure what the wiki has coming its way in the coming months and years, but I'm confident that the wiki will continue to grow and expand by way of everyone's hard work. The wiki has undergone though several changes in the short period of just one year. When I created it, the first article I wrote was Pandora. There was only about one paragraph of information about the film, so I could only edit about 15 articles. As more information poured in, I was able to create more and more articles, and eventually new users came to my aid! That's when Faded-Myth, the second admin, was promoted. Together, we built the wiki together and requested a new skin, that is still being used on the German Avatar Wiki. When the movie was released, the activity went crazy, many users joined the wiki, and as I couldn't handle it all by myself, I promoted more users. After a lot of hard work by the community, the wiki became what it is now, a vast online resource for everything under the sun relating to Avatar.
Now, after 1 year, the wiki has been spotlighted twice, and it is considered as the seventh best Movie Wiki in Wikia. Remember that thanks to all of you this has become possible. Special thanks to our admins and rollbacks, who are our most trusted users.I would just like to say, thank you all for making this wiki such a truly amazing place, and for establishing a community that is one of the most fun-loving and caring communities around. You guys and gals are the heart and soul of this place, and I know that, through your hard work, the wiki will continue to thrive and amass all of the quality information about the thing that brings us all together: Avatar.


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I've heard that the new Slingshot Farmboy is going to have rear sliders. Does anybody else know anything about the upcoming 29er's from Slingshot? Are they just getting sliders, or will there be more rear tire clearance, a longer top tube, any other changes, release dates on the frame?
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Thread: 2010 farmboy?


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Question: Describe the relationship between annual objectives and policies
Describe the relationship between annual objectives and policies.
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Answer to relevant QuestionsIdentify a long-term objective and two supporting annual objectives for a familiar organization. Explain why organizational structure is so important in strategy implementation. Women comprise only 6 percent of corporate board seats in Asia, compared to 17 percent in Europe and 15 percent in the USA. Why is this a problem globally for 1) companies with a low percent and 2) countries with a low ...Why is it essential for organizations to segment markets and target particular groups of consumers? Explain how you would estimate the total worth of a business.
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READY IN: 5mins
Recipe by Ben Ross
Have your own bit of decadence with this drink. Simple yet nice. One of my favourites.
Top Review by Bev I Am
"Decadence", the perfect descriptive word for this drink. Each individual flavor is clearly distinctive. This could easily become favored over dessert ;) I made this using 1 shot of each and served over ice. This I will make again and again! THANK you, Ben Ross!
See Discussion:1 Review
Ingredients Nutrition
- 15 ml Kahlua
- 15 ml Frangelico
- 15 ml Baileys Irish Cream
Directions See How It's Made
- Pour Kahlua first, then Baileys, then Frangelico into a shot glass.
- Shoot.


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♥ Erin
Wednesday, August 1, 2012
At A Crossroads
♥ Erin
16 comments:
- Jamie said...
Hang in there. I think you're making the right choice- give it a week to mull everything over.
- August 1, 2012 at 10:24 AM
- Sarah said...
I really enjoy reading your blog. With that said I understand being a little overwhelmed with life and blogging. I havent been the best blogger over summer because there has been so much going on that take priority over the blog but I like to think my readers understand (if I even have regular readers) Keep your head up!
- August 1, 2012 at 10:25 AM
- Frances @ It's Sew My Style said...
I've been at that point many times. I don't know if you remember, but I took a few months off of my blog. I hadn't gained any new followers in two years, and had about 10 hits per day (most of which were my mother). Sometimes you just have to do things because you like to do them and not worry so much about whether people are reading it or not. Of course it is wonderful to get that interaction with followers and know that you are writing to someone, but that's not everything. If your blog is your creative outlet then just let it be that. I've spent many days crying over my blog, but in the end I came back to it because I enjoy it. If that ever changes I won't hesitate to stop.
So the moral of my essay here lol is that you need to do whatever feels right to you. If you feel you need a break, then take a break. If you feel you need to make some drastic changes, then I'm sure you will find a way in due time to do just that. It will all work out. Keep your head up :)
- August 1, 2012 at 10:30 AM
- momFITtingitallin said...
enjoy your break. The most important thing is you do what makes you happy.
- August 1, 2012 at 11:16 AM
- Neely said...
You already know my thoughts and that i will always support your decisions. I think you need to do what feel s right for you. Dont feel guilty or bad about it.
- August 1, 2012 at 12:38 PM
- Holly said...
I absolutely love your honesty! I've been at this crossroads. I've caught myself getting too caught up in the glitz and glamour of other bloggers and trying to compete. I started being more real. If I don't post every day, so what. It's my blog and I'll do with it what I want. I started letting go of the expectations I had because I was trying to live up to other bloggers. I wasn't happy because I was distracted from what truly mattered to me when it came to my blog. Hang in there. You will figure it out and do what's best for you. Loyal readers will still be there. I'm a perfectionist too, but sometimes something's got to give. Good luck sweet girl! I'll be thinking about you!
- August 1, 2012 at 12:42 PM
- Shelley said...
It's okay to take bloggy breaks!
- August 1, 2012 at 12:43 PM
- Carly Ann said...
I think that if you feel like you need to take a bit of a bloggy break, you should do it. Maybe don't accept anymore sponsors until after you feel like you can devote more time to the blog again, but other than that, I think your followers will still be your followers :)
- August 1, 2012 at 5:29 PM
- tara said...
i have been feeling this exact same way for months! after blogging for almost 3 years, i feel its become a major part of my life and its hard for me to walk away. plus its introduced me to so many amazing people. at the end of may, i refunded all my sponsors who had paid for future months, i just had to stop. i was putting SO much pressure on myself and it took all the fun out of blogging. i don't know what to tell you besides do what's right for you. blog for fun. if you need a break, take one. <3
- August 1, 2012 at 7:57 PM
- Krystal said...
ditto what tara said! blog for yourself and sponsors will be fine just being along for the ride! ;) you think too much about this, i didn't blog 4 days this week but i'm not beating myself up about it. some days i'm better at this blog thing than others. even still - i manage to have sponsors who renew their sponsorships and even upgrade. you just never know!
- August 1, 2012 at 8:58 PM
- MaryBeth said...
Take the summer off. You are your own blog boss right now so nothing you do is wrong. But please come back. I read your blog because you are in Philly. I love Philly blogs because I live right outside of Philly. Supporting the home team.
Do what you must but you are on my reader so if you post I will read.
xoxo, MB
- August 2, 2012 at 12:00 AM
- Kristine said...
I would definitely miss you if you left but I'd hope that we could still keep in contact. I'm glad that we've formed a friendship via blogging, but I definitely know what you're going through. Sometimes it just seems it takes too much effort. Love you E-bomb.
- August 2, 2012 at 12:23 AM
- Cait said...
i know how ya feel darling. i def feel the same way and id be really sad if you left! hang in there! of course you do what you want, because it is YOUR blog. :) xo
- August 2, 2012 at 3:37 PM
- Annmarie Pipa said...
is there something in the air? I have been thinking this same thing every night for the past 2 weeks...then wake up and write another post...
- August 3, 2012 at 10:47 PM
- SKB said...
I was exactly where you are at the beginning of the summer and I had to walk away for a little bit, clear my head, and breathe. I too am not happy with how my blog "looks" right now and think I have decided to take the plunge and get a designer. I have missed talking to everyone so much and I know realize that I have developed some fairly strong relationships just through blogging. I think you have the right idea-walk away for a little bit, clear your head, listen to your heart, and I know you will be back better than ever! Good Luck!
- August 6, 2012 at 3:36 PM
- Kristen said...
so i'm super behind in reading, so i'm glad i'm late bc i already know the answer to this post is that you're not leaving. but i'm sad i'm late that i couldn't have talked to you about it beforehand!
i totally know the feeling. i've been posting less lately and now that the hubs and i are on the same schedules, my blogging is taking time away from him and it sucks. it really makes you thihk about what's important.
- August 7, 2012 at 12:47 AM


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The equity that small business owners build in their companies represents a valuable personal asset. But in a privately-held company, how can equity be converted to liquid cash when the owner exits the business? The key to a profitable exit is to have a succession plan in place. Here are some important details to consider. The Tax Impact at an Owner's Exit
 S uccession planning aims to achieve an optimum outcome for the business (e.g., passing ownership to an heir or selling the company) while also converting business equity into liquid cash when it is needed. This goal is important for two reasons:

 At retirement, business owners usually want to enjoy their leisure time or pursue other opportunities. After giving up control, they don't want to worry about the health of a company they have left behind.
 An owner's heirs may lack the expertise or interest to manage the business. At the owner's death, they would like to receive cash to increase their own personal financial security, and perhaps also to meet income and estate tax obligations.

 The value of a business passed to heirs is included in the owner's estate at death and could be subject to federal estate taxes if not left to the surviving spouse. These taxes must be paid in cash, and the filing deadline for federal estate taxes is nine months after the date of death, unless an extension is requested. So, even if heirs do not need or expect a business to produce immediate cash after an owner's death, federal and state governments require cash to settle taxes. In the worst cases, valuable businesses have been put on the market at "fire sale" prices just to meet estate tax pressures.

 Aside from taxes, heirs may need cash for other needs including business debts and obligations, probate and attorneys' fees, the cost of business appraisals, audits, and the cost of closing down the business and paying severance to employees. Almost every business needs a pool of liquid cash to work through a period of transition in ownership. Providing this cash is one of the most important steps in the succession planning process.

 Three Key Questions
 One starting point for business succession planning is to ask and answer three questions:

 What is the business worth now on a "fair market value" basis? Fair market value is the amount that a willing buyer would pay a willing seller in an arm's-length negotiated transaction. A business appraisal conducted by a qualified professional can help to answer this question.
 What will the business be worth when the owner exits? Any future growth in revenues or profits should increase business value. Owners also can increase value by making provisions to groom one or more successors.
 How will heirs obtain a fair value for the business when the owner exits? A solution called a buy-sell agreement pre-determines the terms of a sale (including transaction price) and also provides the cash necessary to complete the sale and pay expenses and taxes.

 Terms of a Buy-Sell Agreement A buy-sell may be formed between co-owners or partners, who each agree to buy out the other's interest. Alternatively, it may involve the current owner and a designated successor owner, perhaps a family member or top manager.

 Most buy-sell participants lack the resources to buy a partner or owner's interest in a valuable business. Without planning, they may be capable of completing the transaction only by: 1) borrowing heavily; or 2) paying in installments over time. Since most business owners and their heirs prefer to receive cash at the closing, it is necessary to define the source of the cash well in advance. Often, the primary source is permanent life insurance.
 Valuing the Business

 After a successor is determined, the next step is to determine the buy-out value. While small business owners have some flexibility in setting the price of a buy-out transaction, the IRS and courts will insist on a valuation that represents fair market reality, some valuation methods include:

 · Comparable recent transactions – Business value is based on the terms of sales or mergers involving companies of comparable size in the same industry or market area.
 · Multiple of revenue or book value – Business value may be pegged to a multiple of gross revenues in the year or two just before the owner exits. For example, many service-oriented businesses sell for about one to two times annual gross revenues. Or, the value may be pegged to an audited balance sheet as a multiple of "book value."
 · Discounted cash flow —The value is based on total cash flow that the business is projected to generate for a period of years (typically three to five) after the owner's exit, discounted by a cost of capital.
 Hire an Attorney to Draft a Legal Agreement

 The next step is to formalize the buy/sell arrangement through a written agreement with the help of an attorney experienced in succession planning. Ideally, this attorney also has some proficiency in estate tax planning and business valuation. An important section of the agreement defines the "trigger events" that will require ownership to change hands. Common trigger events include an owner's death, disability, retirement, divorce, or separation from employment. When a buyout is triggered by an event other than death, the legal agreement also may include provisions that prevent the departing owner from competing against the company or disclosing its trade secrets.

 Provide Funding to Assure that the Agreement is Carried Out

 Permanent life insurance typically is used to fund buy/sell arrangements because coverage can continue, and premiums remain affordable, at any age. Funding buy/sell arrangements with permanent life insurance also has other benefits:
 · Quick and convenient cash for heirs – Life insurance solves the problem of turning an illiquid asset (the business) into liquid cash for heirs or estate settlement.
 · Tax advantages – Life insurance pays a death benefit that is free of federal income taxes. In buy/sell arrangements, the benefit is usually paid to the party who has the obligation to buy the shares: the surviving shareholders, outside buyer, or the death benefit does not create estate tax consequence for the estate of the deceased.
 · Affordable, level premiums – Permanent life insurance can be purchased at affordable level premiums, especially when the insured person is fairly young and in good health.
 · Cash value —The cash value of a permanent policy can provide buyout funds if an owner exits at a trigger event such as a divorce or normal retirement. Most agreements include provisions for terminating the buy/sell by mutual consent or if specified events occur. In this case, the policy's owner can recoup part of the premium cost from cash value.

 Planning for a Long-Term Disability

 In addition to an owner's death, another trigger event that can be funded with insurance is an owner's long-term disability. In this case, disability income insurance can be purchased to fund an obligation written into the buy-sell agreement. Subject to the terms of the policy, disability buy-out insurance pays to the business beneficiary or other owner a stated amount of lump-sum or periodic income (after a waiting period) that can be used to fund part or all of a buyout.

 In summary, business owners rarely stop working long enough to ask why they are working so hard. But there will come a day when this question will become paramount. Ultimately, a small business may not maximize long-term success for the owner and heirs unless the owner plans ahead to reap the rewards.


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How can I tell if my look needs updating, short of someone actually being honest enough to tell me? I don't have anyone in my life to do this, but I suspect it may be time since I've never considered this question before. Please address hair, makeup, clothes and even fragrance if possible.
Most Helpful Guy
Until this coming Wednesday, I work in retail/menswear. Honestly, I have a lot of guys just straight up ask me "What's the style/what's trending these days?" A lot of these guys haven't been suit shopping in 10+ years.
Honestly, I'd just do that. If you haven't been dress shopping for a while, go to a good store and ask your rep.11|00|0


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Butch Cassidy and the Sundance Kid - as leaders of the Wild Bunch, they planned and executed the most daring bank and train robberies of the day, with a professionalism never before seen by authorities. For several years at the end of the 1890s, the two friends, along with a revolving cast who made up their band of thieves, eluded local law enforcement and bounty hunters, all while stealing from the rich bankers and eastern railroad corporations who exploited western land. The close calls were many, but Butch and Sundance always managed to escape to rob again another day - that is, until they rode headlong into the 20th century.
Fenced-in range, telephone lines, electric lights, and new crime-fighting techniques were quickly rendering obsolete the outlaws of the wide open frontier. Knowing their time was up, Butch and Sundance, along with a mysterious beauty named Etta Place, headed to South America, vowing to leave their criminal careers behind. But riding the trails of Chile, Argentina, and Bolivia, Butch and Sundance would find that crime wasn't through with them just yet.
In The Last Outlaws, Thom Hatch brings these memorable characters to life like never before: Butch, the brains of the outfit; Sundance, the man of action; and the men on both sides of the law whom they fought with and against. From their early holdup attempts to that fateful day in Bolivia, Hatch draws on a wealth of fresh research to go beyond the myth and provide a compelling new look at these legends of the Wild West.
"An easygoing account of the outlaw duo whose era separated Frank and Jesse James from Bonnie and Clyde." (Kirkus)
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Model Mayhem Admin
Stacie HovlandModel Female San Francisco, California, US
My MM URL: http://www.modelmayhem.com/staciehovland
Mayhem # 368964
About Me
It's hard not to have fun when you work in one of the most glamorous and creative industries. I enjoy collaborating with people who take pride in a professional approach and execution of goals while keeping the passion for innovation alive. I am friendly and level headed. Feel free to message me : ) I have great respect for the eye of a photographer and the vision of a MUA.
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At Eastman, people are at the heart of our business. With more than 15,000 employees worldwide and customers in over 100 countries, we know the importance of working together with our team members, communities and organizations to identify issues that matter to us all.
We focus on the right projects and initiatives with the greatest collective impact. We build and strengthen strategic partnerships and challenge ourselves to enable the most positive change.
Corporate Responsibility is more than just philanthropy. It shapes how we operate.


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Nicole Scherzinger passed on movie role
Nicole Scherzinger turned down a movie role because she was asked to go nude.
The former Pussycat Dolls singer says she would only consider stripping off on screen if she could work with Martin Scorsese as she doesn't feel she "needs to" get naked to get a starring role in a film.
Speaking on The Jonathan Ross Show, she said: "Actually I was going to do a movie and they asked me to get nude and I was like no. I mean unless it's like for Martin Scorsese or something but no, you don't need to."
Meanwhile, the 38-year-old singer previously admitted she has "sacrificed" her life for her career.
She said: "Look at me? I'm not married and I ain't got no kids yet. I'm still like, 'Let's go.' Of course work has come at a sacrifice. I've missed out on life."
And whilst Nicole works really hard, her health is the most important to her.
Asked if she'd consider botox, she said: "No, I haven't. I think for me it's more about taking care of yourself from the inside out. I think health is really important.
"It's about drinking a lot of water and getting a lot of sleep. It's crazy what hot yoga or going on an amazing run will do. Anybody can always get that stuff done, but for me how I feel inside is a true reflection."
Nicole is in "a very good place" in her personal life at the moment.
She added: "I'm at a very good place now. I'm really happy. I don't feel like there is any wall up or any tension or awkwardness. But that's what's so great with time and life and experience. What doesn't break you or doesn't kill you really only makes you stronger.
"I wasn't shying away from anything. But I'm not taking any low digs or slaps at my past relationship because that's just not who I am. Music speaks for itself and it's open to interpretation for everyone. But everything I sing, I sing it from my piece of truth."


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sig m400
Anybody have any info on these. I own several sig handguns they are great. I have a dpms lite 16 its the only ar I have lots of experience with and been a really good rifle but planing on buying this sig m400 this week. Getting good deal.
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I have been working on making valentine's day cookies for my daughter's dolls and furry friends. I have over done it (no one needs 5 dozens!) and now I am looking into selling some of these cookies. I have always given away my work, with great pleasure but as I am no longer employed, I find it harder to supply my creative need. Need might be a strong word, but there are so many beautiful fabrics, and Yes I do "need" this beautiful batik, I do not have this purple shade of blue! So may be we can call it addiction, but that might imply it is wrong.... which as a total addict, I will deny!
Tuesday, February 2, 2010
first blog
With the dogs snoring away at my feet, I start this new adventure.... I am not much of a writer so I feel the pressure. I like to make things, not so much talk about them, so probably more pictures than words!
2 comments:
I would not call it an addiction but an amazing creative brain. I think that seeling them is the right thing as the quality of your work needs to be appreciated an enjoyes. Not only you have an amazing taste to pick materials but I always enjoy looking at how wonderfully executed and finished is everything. I'm glad you have a blos so we can enjoy you and your art more.ReplyDelete
thank you Rosa. This should be the perfect place to display all of my creations!!!ReplyDelete
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Outdoor areas can be very functional, mainly whether you take the time to get the most appropriate outdoor furniture to comprehensive these spaces. You can modify these spaces into your personal relaxation and entertainment spaces with all the right furniture. Patio furniture choices are in immense variety today and creating additional considerations for your area is necessary whether you are to get the very best.
One of the things to discuss in outdoor patio furniture is how it will be used and how it fits with your personal sense in furnishings. Even though it's critical to discuss how the furniture fits into your design and available area, the comfort that the outdoor furniture proposes you and your guests is also a factor that need to be considered. Whether you have a deck, porch, or patio space, outdoor furnishings will make it more functional and give you the ability to enjoy this area comfortably.
You will find these outdoor patio furniture takes hold most department stores. Prices can differ greatly according to the style of materials and how many pieces can be found in the set. For some of the more stylish and trendy designs, you really should research in order to find your deck furniture online. The shops that might be over the Internet it is possible to find difficult to find styles and designs that will certainly make your patio area an excellent destination for a have fun.
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