text:
Tight associations between transcription promoter type and epigenetic variation in histone positioning and modification
Transcription promoters are fundamental genomic
Here, we performed a systematic analysis of transcription promoters and gene expression, as well as of epigenetic histone behaviors, including genomic position, stability within the chromatin, and several modifications. We found that, in humans, broad promoters, but not peak promoters, generally had significant associations with nucleosome positioning and modification. Specifically, around broad promoters histones were highly distributed and aligned in an orderly fashion. This feature was more evident with histones that were methylated or acetylated; moreover, the nucleosome positions around the broad promoters were more stable than those around the peak ones. More strikingly, the overall expression levels of genes associated with broad promoters (but not peak promoters) with modified histones were significantly higher than the levels of genes associated with broad promoters with unmodified histones.
These results shed light on how epigenetic regulatory networks of histone modifications are associated with promoter architecture.
Recent progress in high-throughput technologies has made it possible to collect a variety of "omics" data on transcripts and on the epigenetic behaviors of the histones that are often associated with these transcripts
Cap analysis of gene expression (CAGE) is a high-throughput method that enables large-scale identification of transcription start sites (TSSs) of eukaryotic species. This method measures gene expression levels simultaneously with TSS identification by counting the sequenced 5' ends of full-length cDNAs, termed CAGE tags
In eukaryotic species, chromosomal DNAs is packed into nucleosomes, each of which comprises approximately 147 base pairs wrapped around a histone protein octamer consisting of two copies of each of the four core histones, H2A, H2B, H3, and H4
H3K9ac has recently been frequently observed around broad promoters
Promoter architecture and nucleosome positioning
We first focused on differences in nucleosome distribution around the two different types of transcription promoters (i.e. peak and broad promoters). We used human promoter positions for which information about the degree of transcription start imprecision had been obtained in a previous study
Figure 1
Distributions of nucleosome positions around transcription start sites (TSSs)
Distributions of nucleosome positions around transcription start sites (TSSs). (A) Distributions of the central positions of histone H3 around broad and peak promoters. The
H2A.Z is a histone variant of H2A that is highly conserved among lower and higher eukaryotes. Enrichment of H2A.Z around the promoter region has been also reported in yeast
Accessibility of transcription factor Sp1
The two promoter architectures are associated with characteristic sequence contexts: the peak promoter is located close to a TATA box and the broad promoter close to CpG islands
Additional file 1
Supplemental figure 1. Figure S1. Frequencies of occurrence of TATA boxes and CpG islands around transcription start sites (TSSs). Frequencies of the characteristic sequence patterns associated with promoters are shown by bar charts. The
Click here for file
It is possible that the aligned patterns of nucleosome positions around broad promoters are due to the accessibility of transcription factors to DNA. For instance, in the absence of the TATA box, the ubiquitous transcription factor Sp1 can recruit TATA-binding proteins to initiate transcription
Additional file 2
Supplemental figure 2. Figure S2. Distributions of Sp1 sites around transcription start sites (TSSs). Distributions of nucleosome regions and Sp1 sites around TSSs associated with broad (A) and peak (B) promoters are shown. Nucleosome position is defined as the center position of the nucleosome. The
Click here for file
Additional file 3
Supplemental figure 3. Figure S3. Distributions of the binding sites of two transcription factors (MAZ and PU.1) around transcription start sites (TSSs). Distributions of nucleosome regions and transcription factor binding sites around TSSs associated with broad (A: MAZ, C: PU.1) and peak (B: MAZ, D: PU.1) promoters are shown. Nucleosome position is defined as the center position of the nucleosome. The
Click here for file
Positional stability of nucleosomes around broad promoters
If nucleosome positioning around broad promoters confers DNA accessibility for the binding of transcription factors, then the nucleosome positions around broad promoters should be more stable throughout different cellular conditions than those around peak promoters, because broad promoters are usually associated with ubiquitously expressed gene (in contrast, peak promoters are associated with tissue- and condition-specific expressed gene)
Distribution of nucleosomes containing modified histones
It has been suggested that not only nucleosome position, but also nucleosomal histone modification, can regulate transcription
Figure 2
Distribution of modified histones around transcription start sites (TSSs)
Distribution of modified histones around transcription start sites (TSSs). Distributions of nucleosomes containing methylated and acetylated histones. (A) H3K4me1, (B) H3K4me2, and (C) H3K4me3 and (D) H3K9ac around TSSs are shown. All of the modified histones were highly enriched around the TSSs associated with broad promoters, unlike those associated with peak promoters. The
Figure 3
Relative abundance in histone distributions
Relative abundance in histone distributions. Normalized differences in histone distributions (H3, H3K4me3, H3K9ac, and H2A.Z) between broad and peak promoters (from -2 kb to 2 kb) at each position are shown. The
Analysis of another genomic element that potentially influences histone behavior
Methylation of CpG islands is tightly associated with the expression of downstream genes; a number of studies have therefore been conducted to analyze CpG islands at a genome-wide level
Figure 4
Distributions of nucleosomes around transcription start sites (TSSs) with and without CpG islands
Distributions of nucleosomes around transcription start sites (TSSs) with and without CpG islands. Distributions of nucleosomes containing H3K4me3 (A, B) and H3 (C, D) around broad and peak promoters are shown. The analyses were conducted separately for TSSs that were associated with CpG islands (A, C) and those that were not (B, D). Broad promoters had aligned patterns of nucleosomes containing H3 and H3K4me3, regardless of the existence of CpG islands, and were enriched in H3K4me3. In contrast, peak promoters had little alignment of the H3 pattern, regardless of the presence of CpG islands. The proportion of nucleosomes containing H3K4me3 associated with peak promoters was lower than that associated with broad promoters, particularly in the absence of CpG islands.
In contrast, when we focused only on promoters without CpG islands, nucleosomes having H3K4me3 were well aligned and enriched only around broad promoters (Figure
Effect of histone modification on gene expression
To explore whether histone modification around the promoter affects gene expression, we analyzed the difference in expression levels of RNAs transcribed from peak and broad promoters in terms of the existence of modified/unmodified histones in their surrounding regions. We compared data sets of methylated/unmethylated histones and acetylated/unacetylated histones measured under resting conditions in human CD4+ T cells
Figure 5
Box plots of gene expression in human resting CD4+ T cells
Box plots of gene expression in human resting CD4+ T cells. The box plots represent the distributions of gene expression levels. Distributions of the four groups of genes are drawn separately, i.e. those with broad or peak promoters, each of which was further associated with modified histones in activated cells or with unmodified histones. The
We analyzed the global landscape of epigenetic relationships between histone modifications and transcription initiation by investigating genome-wide ChIP-Seq data and DeepCAGE data. The results presented here show differences in the architecture of the broad and peak promoters that regulate gene expression. Especially, we revealed that the broad promoters were strongly associated with histones immediately downstream of the TSS and they were frequently modified, presumably to regulate gene expression levels.
In previous studies, aligned patterns of nucleosome positions around TSSs have been identified in yeasts and humans
Our data indicate that the nucleosomes that are immediately downstream of TSSs and associated with broad promoters are positioned in specific regions. We suggest that broad promoters have these aligned patterns of nucleosome positions around TSSs because the nucleosome position has a stronger impact on broad promotors than on peak promoters on the determination of TSSs by transcription factors in the cell.
As an example of transcription factors that target broad promoters, we investigated the Sp1 binding sites around TSSs. Sp1 recognizes binding region of DNA via its zinc finger domain whereas TBP recognizes TATA box via its DNA binding domain. Sp1 binding sites were enriched in the regions upstream of TSSs corresponding to the nucleosome-free regions. We observed similar tendencies for the binding sites of two transcription factors, PU.1 and MAZ. Although biological experiments are necessary to investigate molecular mechanism behind this observation, we speculate that the nucleosome-free regions serve as "landing sites" for transcription factors, including Sp1, which have less precise binding motifs (which are overrepresented among broad promoters) than the TATA box
In addition to histone H3, we also analyzed the positions of the histone H2A variant H2A.Z, which is enriched around TSSs
In our analysis of epigenetic control by histone modification, we uncovered an difference between broad and peak promoters. H3K4me1, -2, and -3 and H3K9ac, which are associated with gene activation, were more highly enriched around TSSs associated with broad promoters than around those associated with peak promoters. Thus broad promoters appeared to be under stronger epigenetic control than peak promoters. We found a trend that further supported this hypothesis: the expression levels of genes associated with broad promoters that had modified histones had higher expression levels than genes associated with broad promoters without modified histones. In contrast, peak promoters appeared to be under weaker epigenetic control, because far fewer of them harbored modified histones. Furthermore, there were no significant differences in the expression levels of genes associated with peak promoters that harbored or did not harbor modified histones.
It has been shown that promoters with many CpG islands are more likely to harbor modified histones than promoters with fewer CpG islands
Genes associated with broad promoters tend to be expressed ubiquitously, whereas those associated with peak promoters are likely to be expressed in specific tissues and may show low expression levels in most tissue types
Additional file 4
Supplemental figure 4. Figure S4. Distributions of expression levels of genes selected for comparison of broad and peak promoters associated with similar downstream gene expression. The box plots represent the distributions of the microarray intensities of the gene sets that were selected from among those associated with broad and peak promoters and that had similar expression levels (from 250 to 750).
Click here for file
Peak promoters containing the TATA box are regulated at their transcription initiation step, generally by the assembly of a pre-initiation complex with three additional components: the TATA-associated factors, the so-called mediator complexes, and positive and negative cofactors. We presume that peak promoters containing no TATA box are regulated in a similar way. This transcription system is widely used in various species, and our results suggest that it is unlikely to use epigenetic controls. Thus, broad and peak promoters have distinct systems to regulate gene expression.
Throughout this work, we employed widely-accepted definition of peak promoters, i.e. those which initiate transcription within the range of 4 bps. Changing this threshold to 10 bp did not have much effect on the distribution patterns of nucleosomes around broad and peak promoters as shown by Pearson's correlation coefficients between histone distribution pattern around broad promoters (-5000 to 5000 bps with respect to TSS) defined by > 4 bps threshold and that defined by > 10 bps threshold. For H3 distribution patterns, correlation coefficients were 0.99 and 0.94 for broad and peak promoters, respectively. For H3K4me3 distribution patterns, the correlation coefficients were 0.99 for both broad and peak promoters. These results suggest the robustness of the relationships between the imprecision of TSS and patterns of histone distributions.
TATA boxes are used in a wide range of organisms, including prokaryotes, and are thought to be part of an ancient transcriptional system. In contrast, broad promoters are thought to be newly evolved
By using a computational approach, we discovered the general relationships between the two types of promoter architecture and histone behavior, including positioning and modification. We first showed that the positions of histones around broad promoters were highly aligned and stable compared with those around peak promoters. Furthermore, we suggest that marked numbers of transcription initiations related to broad promoters are under the control of histone methylation and acetylation and are associated with gene expression level, whereas this is not the case with peak promoters. These results indicate that the expression of genes associated with broad promoters, but not peak promoters, is highly associated with histone position and modification. We believe that our study is a step in uncovering the general mechanisms underlying transcriptional systems and inferring how these systems have evolved. This should eventually help us to understand the complexity of mammalian transcription.
Nucleosome position detection and dataset
Nucleosome-resolution (MNase digestion) ChIP-Seq Solexa tags for histone H3 were obtained by
Transcription start site detection and dataset
TSSs were detected by DeepCAGE data obtained by the FANTOM 4 project
Distribution of nucleosome positions around TSSs
As described above, the genomic positions of nucleosomes as well as TSSs for both broad and peak promoters were determined. The distributions of nucleosomes within the genomic regions from -5 kb to 5 kb with respect to TSSs were calculated by dividing the number of nucleosomes at each position by the number of TSSs. Genomic positions from -15 bp to 15 bp with respect to the central positions of the nucleosomes were assumed as the genomic positions where nucleosomes existed. The distributions of nucleosomes near broad and peak promoters were calculated separately.
Distribution of Sp1 binding sites and other transcription factor binding sites
Sp1, MAZ, and PU.1 binding sites were obtained from FANTOM 4 (
Stability of nucleosome positions under different cellular conditions
We compared the nucleosome positions obtained in human resting CD4+ T cells with those obtained in human activated CD4+ T cells. We calculated the minimum distance between each nucleosome in resting T cells and the closest nucleosome in activated cells. This distance was considered to denote how far each nucleosome moved along the genome in response to the change in cellular condition (from resting to activated). The distributions of these distances were calculated by dividing the number of nucleosomes that moved specified distances (from × bp to × + 15 bp) by the total number of nucleosomes. The average absolute minimum distance between each nucleosome in resting T cells and the closest nucleosome in activated cells was also calculated.
Relative abundances of peak promoters and broad promoters
The abundance of peak promoters relative to that of broad promoters at position
Gene expression in human resting CD4+ T cells
The gene expression profile in human resting CD4+ T cells was obtained from the Gene Expression Omnibus (GSE10437)
To compare the distributions of nucleosomes that had H3K4me3, were located upstream of TSSs (positions from -150 to -100 bp), and were associated with either broad promoters or peak promoters in cases where the downstream genes showed similar expression levels, we selected 1788 genes associated with broad promoters and 138 associated with peak promoters that had expression levels in the range of 250 to 750 (Additional File
Authors' contributions
TN performed all computational analyses. TN, NY, and RO designed the study and helped with the computational analyses. AK provided some data. RS supervised the research and helped to interpret the data. MT supervised the study. TN, NY, and RS wrote the paper. All authors read and approved the final manuscript.
This research was supported by research funds from the Yamagata Prefectural Government and Tsuruoka City, Japan, and by a Grant-in-Aid from the Japan Society for the Promotion of Science (JSPS) to NY (grant no. 1924).
The transcriptional network that controls growth arrest and differentiation in a human myeloid leukemia cell line
The transcriptional landscape of the mammalian genome
High-resolution profiling of histone methylations in the human genome
Dynamic regulation of nucleosome positioning in the human genome
Combinatorial patterns of histone acetylations and methylations in the human genome
Cap analysis gene expression for high-throughput analysis of transcriptional starting point and identification of promoter usage
Linking promoters to functional transcripts in small samples with nanoCAGE and CAGEscan
Genome-wide analysis of mammalian promoter architecture and evolution
Core promoter structure and genomic context reflect histone 3 lysine 9 acetylation patterns
A code for transcription initiation in mammalian genomes
Dynamic usage of transcription start sites within core promoters
Transcriptional and structural impact of TATA-initiation site spacing in mammalian core promoters
Evidence for functional binding and stable sliding of the TATA binding protein on nonspecific DNA
DNA-dependent divalent cation binding in the nucleosome core particle
Crystal structure of the nucleosome core particle at 2.8 A resolution
Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome
Human DNA methylomes at base resolution show widespread epigenomic differences
Chromosomal landscape of nucleosome-dependent gene expression and silencing in yeast
Genome-scale identification of nucleosome positions in S. cerevisiae
Nucleosome positioning and gene regulation: advances through genomics
ChIP-seq: advantages and challenges of a maturing technology
A barrier nucleosome model for statistical positioning of nucleosomes throughout the yeast genome
High-throughput mapping of the chromatin structure of human promoters
Epigenetics of human T cells during the G0-- > G1 transition
Genomic maps and comparative analysis of histone modifications in human and mouse
The genomic landscape of histone modifications in human T cells
Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin
Histone modification levels are predictive for gene expression
Regulation of gene expression by transcription factor acetylation
Localized domains of G9a-mediated histone methylation are required for silencing of neuronal genes
A unifying model for the selective regulation of inducible transcription by CpG islands and nucleosome remodeling
Comparative analysis of H2A.Z nucleosome organization in the human and yeast genomes
Translational and rotational settings of H2A.Z nucleosomes across the Saccharomyces cerevisiae genome
H3.3/H2A.Z double variant-containing nucleosomes mark 'nucleosome-free regions' of active promoters and other regulatory regions
Weight matrix descriptions of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences
The UCSC Genome Browser Database
The RNA polymerase II core promoter: a key component in the regulation of gene expression
Large-scale human promoter mapping using CpG islands
A mammalian promoter model links cis elements to genetic networks
Genome-wide analysis of histone modifications in human pancreatic islets
A genomic code for nucleosome positioning
The logic of chromatin architecture and remodelling at promoters
CpG islands--'a rough guide'
Local DNA stretching mimics the distortion caused by the TATA box-binding protein
Keep your fingers off my DNA: protein-protein interactions mediated by C2H2 zinc finger domains
Two strategies for gene regulation by promoter nucleosomes
CpG island mapping by epigenome prediction
Identifying positioned nucleosomes with epigenetic marks in human from ChIP-Seq
JASPAR: an open-access database for eukaryotic transcription factor binding profiles


text:
Hellenic Hydrocarbons Management Committee successfully completed its work regarding the evaluation of the bids for the exploration and exploitation of hydrocarbons for the Katakolon Block. Katakolon was the third region in Western Greece included in the Open Door bid round, launched by the Greek Ministry of Energy, Environment and Climate Change on January 2nd, 2012.
According to the Committee's recommendation to Minister Yiannis Maniatis, the consortium of Energean Oil& Gas/Trajan Oil & Gas/ Schlumberger is awarded the offshore block of Katakolon.
ENERGEAN Oil & Gas
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text: I started using Wifi exclusively at home and my office after reading about the benefits on here. I noticed a TREMENDOUS increase in battery life. But when I'm on Wifi at my gf's parents house, the battery drain isn't even about the same as 3G it seems like it is MUCH worse. What could be causing this? The signal strength is often better at this locaton than home/office so that shouldn't be the problem.


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The Murlpirrmarra Connection's Wiluna Martu Eagles Cultural Football Weekend in Wiluna, 30 May – 2 June 2014
Planning is underway for the sixth annual Murlpirrmarra Connection's cultural football carnival between the Wiluna Martu Eagles and the Dalkeith Nedlands / Subiaco Giants Junior Football Club.
This year's carnival will take place under lights in Wiluna over the long weekend of 30 May – 2 June 2014. The yearly carnival brings together young players from the Wiluna Martu Eagles and the Dalkeith Nedlands Junior Football Club in a unique team-building weekend that combines highly skilled football with educational activities and plenty of fun for all.
The collaborative event was conceived in 2009 by Michael Wilson (Golden West Resources / Murlpirrmarra Connection Director) and Peter Dawson (Murlpirrmarra Connection Director).
The first encounter between these two vastly different locations and cultures was in 2009 and took place at the Dalkeith Nedlands Junior Football Club's home ground in Dalkeith with the Wiluna Martu Eagles team and supporters making the 1200km trip to Perth. 2010's event took place in Wiluna, 2011 was held at Rottnest Island, 2012 was held back in Wiluna and last year's Cultural Football Weekend was conducted in Dwellingup.
It has rapidly become a major event on the junior football calendar and an important celebration of team spirit, cultural understanding, respect, and healthy living.
Last year's event – "The Wiluna Martu Eagles Cultural Football Weekend 2013" - took place in Dwellingup and its surrounding areas and was enjoyed by over 110 players, parents and supporters. The weekend was packed with activities, including team building exercises, cultural awareness sessions, and tours of the area surrounding Nanga Bush Camp.
The annual carnival culminates with the highly-anticipated football match between the two young teams and the presentation of the Wilson–Dawson Perpetual Cup.
The 2014 event partners who have most generously contributed to travel, accommodation, facilities and activities include:
BHP Billiton, Global Construction Services, GWR Group, SMS Rental, Toro Energy, Blackham Resources, Rosslyn Hill Mining, Cameco Australia, Newmont, Reclink Australia, CDNTS and the Shire of Wiluna.
Former World Heavy Boxer, Joe Bugner attended and supported this event 2009 through 2011 inclusive
and former WAFC Chairman Grant Dorrington and board member Larry Kickett will be attending this year's event as they have for the past two.
File Library
Contact Profile
Murlpirrmarra Connection
Murlpirrmarra Connection's mission is to engage Wiluna's Indigenous youth in educational facilities by building strong relationships with Wiluna families, encouraging, supporting and
offering the children educational options. In addtion, Murlpirrmarra Connection creates and implements community initiatives to increase the wellbeing and health of Wiluna's population.
Darren Patten
W: www.murlpirrmarra.com.au/


text:
"I spent my time mostly walking around Shanghai (and as you can see from the shots there was a little Beijing in there as well), listening to punk rock on my walkman (heh, I remember frequently imagining that, all across the long, long history of the country, I was probably the first person to listen to, I dunno, Government Issue or the Mortal Micronotz there), meeting people who wanted to exchange money or practice their English on me — overall just having a really eye-opening & cool time."This was back in the days before he was a fully-fledged photographer of course and he also explains how he came to be able to document the trip so well:
"I borrowed my girlfriend's mother's camera for the trip (a Canon with a nice zoom lens, vastly superior to my own $95.00 "Albinar" 35mm) and after cradling it with reverent care for 3 months I foolishly packed it into my checked bag for the flight back to the states. It was promptly stolen of course (sorry Emily!), but luckily enough the undeveloped film canisters were left untouched."


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The Teen Heads Up Conference is a unique experience for teenagers ages 13-18 who have been diagnosed with a brain tumor and are dealing with long-term effects of both their disease and its treatment. Being a teenager has plenty of challenges as it is; trying to find your way in the world through growth spurts and hormones and first dates and cliques and awkwardness. Now, add a brain tumor.
To help teen brain tumor survivors address these unique challenges Teen HUC offers a combination of the usual camp fun like swimming, archery, dances, horseback riding and high ropes with educational workshops that address specific issues faced by brain tumor survivors. Participants tackle issues head on; education and employment challenges, stunted growth, mood swings, bullying, relationships and deaths of friends with cancer. This conference focuses on developing social skills and giving participants the opportunity to connect with other survivors that may be experiencing similar experiences.
Most important, campers stay connected with one another. They share photos on Facebook, text about treatments, issues and life in general. They leave Camp with a network of friends who understand, provide support and steel their courage long after they've returned home; a network of friends uniquely like themselves.


text:
Jordan is the Director of Marketing at Fundrise.
Prior to joining the Fundrise team, Jordan worked as an Account Manager at Blue State Digital, working with a variety of clients including the Green Bay Packers and Google Take Action.
Jordan received her B.A. in Communications and Urban Studies from the University of Pennsylvania.


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CANADA: GM truck plant re-tool under way
General Motors of Canada has confirmed it is proceeding with a new C$227 million investment in its Oshawa truck assembly centre to prepare for the next generation full size pickup trucks, the Chevrolet Silverado and GMC Sierra.
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Cartagena ipecac
Definition of Cartagena ipecac
: ipecac derived from a Brazilian tree (Cephaelis acuminata) —called also Panama ipecac
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Doctor Appointment booking Practo / ZocDoc / ( iphone / Android )
Budjetti $250-750 USD
We are looking for a expert who already has such software like doctor appoitment booking type, if you have please let us know? mainly we need apps only.
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Integration Best Practices for Business Service Management Community Blog
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New Integrations in WebSphere DataPower 6 and WebSphere Service Registry and Repository 22.214.171.124
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ILO classes scheduled for May 2013
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Article: Integrating WSRR and WESB or WPS
DavidSeager Tags: service registry endpoint wsrr repository lookup wps websphere 3,907 Views
I spotted an article describing using the lookups from Process Server into WSRR. This article shows you how to perform dynamic endpoint lookup by integrating WebSphere Service Registry and Repository V8 (WSRR) with Process Server, using the Endpoint Lookup and...Modified on by DavidSeager
New WSRR article: High Availability caching with Out of the Box WSRR Nodes in IIB flows
DavidSeager Tags: high availability iib wsrr 1,588 Views
A new article about WSRR and IIB has been posted. Titled "High Availability caching with Out of the Box WSRR Nodes in IIB flows" this documents the build and test of a high availability scenario in an IIB message flow. Check it out here:...Modified on by DavidSeager
Video: Installing WSRR V8.0
DavidSeager Tags: registry wsrr repository youtube install websphere service video 3,236 Views
A new video has been posted on the service registry YouTube channel . This one shows how to install WSRR V8.0 on a machine with DB2 already installed. The installation shows using IBM Installation manager in GUI mode to install the WSRR files,...
Spotlight on IBM software solutions: Three-part podcast on SOA governance
Scott Laningham Tags: soa wsrr governance spotlight 9 Comments 5,027 Views
Three IBM software experts talk concepts and tools around SOA governance. Segments offer an overview of SOA governance and related IBM support services, an introduction to WebSphere Service Registry and Repository, and a look at SOA governance and...
from Blog: Scott Laningham
WSRR V8.0 Fix Pack 2 (188.8.131.52) & WebSphere DataPower 6 New Features
DavidSeager Tags: 184.108.40.206 fix 2 pack 8.0 websphere repository 6 datapower 8002 service wsrr registry 4,658 Views
WebSphere Service Registry and Repository V8.0 Fix Pack 2 has been released (126.96.36.199). Among the service fixes, there are new features added by the development team. Included is the subscriptions feature I previously talked a...Modified on by DavidSeager
Demo: Load Documents Wizard
DavidSeager Tags: soa documents governance registry repository wsrr load service 3,400 Views
A new demo has been posted to our YouTube channel , this one shows off the Load Documents wizard in WebSphere Service Registry and Repository. When loading documents which describe a service, such as WSDL or XSD, they often rely upon each other. For example,...
New WebSphere Service Registry and Repository articles: Promotion performance & Custom subscription notifier
DavidSeager Tags: websphere articles soa repository wsrr service registry governance 3,871 Views
A couple of new articles about WebSphere Service Registry and Repository have been posted on developerWorks. Let's take a look. Promotion performance in WebSphere Service Registry and Repository The promotion of web service metadata to different test and...
InterConnect 2016 presentation
DavidSeager Tags: service 2016 wsrr interconnect registry repository 1,772 Views
After InterConnect 2016 the presentations are made available online, and my session is no exception. Click the link below to see the deck from: Soar into the API Economy by Moving Services and Metadata from&nbs...


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FIFA 14 is on the first place
FIFA 14 is on the first place in Cheap fifa 15 coins first rank multi-format 2014 in the UK, making it also one of the most important releases at the end of last year.
Call of Duty : Ghosts sits in second place, ahead of Assassin's Creed IV: Black Flag , Battlefield 4 and Grand Theft Auto V . In fact, the top seven best-selling games all occupy the same positions as last week. Static.
Ryse: Son of Rome (and Saints Row IV ) back in the early twenties, while it should be noted that the exclusive offers from Sony for the PS4 ( Killzone: Shadow Fall and Knack ) are not even in the rankings.
There is very little movement this week, but let's see in more detail how you inaugurate the UK charts with the top 20 of 2014:


text:
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FP&A Supervisor
Boca Raton , Florida
March 31, 2017
Job Description:
This role will be an integral part of the FP&A team, providing financial analytical support to the Commercial Excellence Team. The candidate will work in partnership with the business functional leads in ops reviews, commercial and functional reporting and ad hoc analysis needs. There will be some project specific work as well. Travel will be 10-15%.
Provide financial sales support including sales productivity, quota setting, revenue and margin projections
Responsible for meeting Commercial financial commitments, developing and driving affordability and productivity models, and various ad-hoc financial analysis to support key business decisions
Maintain close alignment with the business partners and Sales functional leadership to fulfill analysis requirements
Maintain understanding of business operations/environment within the team in order to provide Operating Review commentary
Participate in special projects to improve reporting, analytical tools and internal processes to enhance management decision process
Drive simplification effort across global/regional commercial support to drive standardization and best practices (e.g. via data feeds, process improvements, etc.)
Responsible for budgeting/forecasting of appropriate functional costs
Drive the creation of process documentation for activities which reside within team
Demonstrated financial analysis and modeling expertise
Job Qualifications:
Bachelors in Finance, Accounting, or equivalent degree required. MBA, CPA, or other advanced certification preferred
5+ years or equivalent experience, focused on functional support, budgeting, forecasting, and financial analysis & modeling
Commercial Excellence experience preferred
Analytical, hardworking, driven, opportunity seeking, quick learner
Proficient in MS-Office,
Proficiency in Essbase and Hyperion Financial Management preferred but not required
Exceptional organizational skills with the ability to manage multiple competing priorities while consistently meeting deadlines
Ability to provide thought leadership to shape future decisions
Ability to manage processes to deliver required results
Ability to work independently, and proactively develop solutions with minimal guidance
Capacity to analyze complex problems and make decisions in a timely manner
Strong oral and written communication skills and comfortable interacting at all levels of the organization
Ability to work in a highly-matrixed organization
Business acumen (Tyco preferred)
Periodic travel required
The position requires individuals to have high energy, be extremely analytical and be able to quickly grasp complicated issues. You should also be comfortable working in a matrix
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Johnson Controls is a global diversified technology and industrial leader serving customers in more than 150 countries.
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422 Studio Photographer
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I'm a professional makeup artist available for fashion, media, editorial, runway, headshots, weddings, etc.
My rates are reasonable.
***Doing very selective TFP/TFCD***
Please contact me for my rates.
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Jeanne Moreau
Soul Urge (Heart Desire) Number 8
Jeanne Moreau is an Actress.
She was born on Monday January 23rd 1928, in Paris, France.
Jeanne Moreau wants success in its fullest meaning - wealth, power, and material comforts. She has an enormous ambition. Jeanne dreams of big projects, great undertakings, and rewards.
Moreau is a visionary. She sees the horizon and the promise. In general, Jeanne Moreau also sees the methods necessary to fulfill that promise. But she is not especially good with details; Jeanne needs others to help her deal with the smaller parts of the picture. Her challenge is to make full use of the full spectrum of her abilities, as indicated by her other core numbers. In the same way, Moreau must bring forth the best from others and orchestrate their talents toward the realization of her vision. In short, Jeanne Moreau must lead by example, demonstrating the standard for commitment, determination, and excellence.
All of this requires effort on Jeanne Moreau's part. She will meet with obstacles and difficulties. Her determination and commitment will be tested. But Jeanne has the power to overcome every obstacle she faces and accomplish the goal she has set for herself.
Jeanne's task in life is to learn to use power in refined and elevated ways. The expression and use of power is directly related to One's personal evolution. The more primitive uses of power rest with violence and the threat of deprivation. The higher expressions depend upon Moreau's capacity to care for and nourish the people and projects in her charge, giving each exactly what is needed at the appropriate time and in appropriate amounts.
Jeanne Moreau has a creative mind and an unusual approach to business and problem-solving. She needs, however to cultivate her ability to evaluate others.
It is essential for Jeanne to be involved in a project that challenges her and offers the potential for rewards. When she is uninvolved or unoccupied with a worthwhile task, Moreau can become deeply despondent, depressed, and frustrated. Jeanne Moreau is the perfect example of the old cliche, "Idle hands are the devils workshop." Without a challenge, she can lose balance in life and become selfish, cruel, and even self-destructive. Disappointments become shattering, causing terrible consequences in self-image and self-love.
Perspective is everything for Jeanne Moreau. She has a natural talent for balancing the spiritual and material planes.
Jeanne needs to cultivate courage and stamina. Courage is a choice. Jeanne Moreau needs to decide to be brave in the face of fear. A balanced 8 is among the most powerful and satisfying of numbers. It is the spirit of the true survivor, able to bounce back and achieve a greater victory.
You and Jeanne
About Jeanne's Soul Urge (Heart's Desire) number
Jeanne Moreau's Soul Urge number represents her inner self. The Soul Urge number, also called Heart's Desire, shows Jeanne's underlying urge - her true motivation.
It reveals the general intention behind many of Moreau's actions. Consequently, it dramatically influences the choices Jeanne Moreau makes in life.
Read more about our interpretation of Jeanne Moreau's soul urge (heart's desire)


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Your Dodge dealership isn't the only place to locate genuine or original equipment quality parts to repair your D250. Make no mistake, you didn't buy your D250 unless you wanted to buy great performance and great value both impeccably combined into one automobile. You bought your D250 because its dependability and versatility agreed with your nature; so why would you be okay with second best when it comes to the highest quality auto parts? Auto enthusiasts appreciate the aggressive styling built into each Dodge, and know that only top quality repair D250 parts will suffice when repairs are needed.
The need to heat the interiors our vehicles came before our ability to cool them. The heater blower motor in your Dodge D250 is installed on the passenger's side of the firewall, and can usually be accessed through the glove box or engine compartment (depending on the vehicle). The job of your Dodge D250 heater blower motor is to propel a cone of air past your heater core and into the passenger compartment. If your heater blower stops spinning, then you can be sure cold days are ahead inside your car or truck.
At Car Parts Discount, we have live customer service members on the phone with enough expertise to help you select the appropriate Dodge D250 part for your needs. When your Dodge needs the best replacement Blower Motor, there's no need to go somewhere besides the authority, Car Parts Discount. Bring your D250 back to life with the best new or rebuilt parts from the experts at Car Parts Discount. If your car or truck was built in 1993, 1992, 1991, 1990, 1989, 1988, 1987, 1986, 1985, 1984, 1983, 1982, 1981, then you will find the correct Dodge D250 Blower Motor parts right here.


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It's something that I've been wanting to do for awhile now?
Would I look ridiculous?
Most Helpful Girl
Bottom line it has to be your desicion. But, food for thought...years ago, I thought about it for a long time and finally just went for it, and I looked HORRIBLE. My hair is naturally dark brown and it just looked SO unnatural on me. My profile pic looks sort of like my hair might be blonde, but really it's light brown there, the cartoon effect thing I did to it, lightened. And once you lighten it, it's sometimes hard to get it to hold dark colors again for a while. I'm just letting you know my experience, but again, that decision rests in your hands.00|00|0


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The Red Gate Bridge is a large red bridge on the south end of Wuhu Island. It bears a resemblance to the real- life Golden Gate Bridge. It first appeared in Wii Fit as a structure that you run upon during jogging, but was given its name in Wii Sports Resort. It is the largest suspended bridge on Wuhu Island. It links Town and the Marina to Palm Boulevard, Cocoba Hotel, the 3 Beachs, Sweet Beach, Starry Beach and Sugersand Beach, and Island Loop Tunnel No.1.
- The Red Iron Bridge could be only seen in Cycling and Island Flyover.
- The Red Iron Bridge is the only bridge in Wuhu Island.


text:
Best Buy has now started selling the spare or replacement charger for the Moto 360 smartwatch through its retail outlets across the U.S. Although the Moto 360 comes with a charger of its own, customers can have a spare charger in handy for work or if they travel frequently. Since this isn't an ordinary charger, Motorola is charging a hefty $39.99. This is understandable given that it is based on the Qi standard. However, there are multiple Qi based chargers available elsewhere for a lot cheaper, which is worth a look as well.
Best Buy is only offering in-store pickup for this wireless charger which means there's no home delivery available. So make sure you walk into the nearest Best Buy outlet for more details on the spare charger for the Moto 360.
At $40, the Moto 360 wireless charger is relatively expensive compared to conventional charging accessories. Considering that the charger cannot be used for anything other than the Moto 360, it might be better to look at other Qi based chargers. Hopefully, Motorola will bring the price down in the future to make this a more attractive proposition to Moto 360 owners.
Source: Best Buy
Via: Talk Android


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|Church positions
|Archdiocese of Lyon
|Archbishop of Lyon, Cardinal priest of Sant'Eusebio
|Period in office
|May 26, 1887 — January 23, 1893
|Pierre-Hector Coullie
|Previous post
|Archbishop of Besançon
|Created cardinal
|May 24, 1889
|Date of birth
|April 29, 1823
|Place of birth
|Paris, France
|Date of death
|January 23, 1893 (aged 69)
|Place of death
|Lyon, France
Joseph-Alfred Foulon (April 29, 1823 – January 23, 1893) was a French Roman Catholic Cardinal and Archbishop of Lyon.
He was born in Paris and studied in the Saint-Sulpice Seminary. He was ordained priest on December 18, 1847, in Paris, where he taught for twelve years in the minor seminary.
He was elected bishop of Nancy on March 27, 1867. He took part in the First Vatican Council. He was promoted to the Metropolitan See of Besançon on March 30, 1882, and transferred to the Metropolitan See of Lyon on May 26, 1887.
Pope Leo XIII created him cardinal priest in the consistory of May 24, 1889 with the title of Sant'Eusebio.
Cardinal Foulon died on January 23, 1893, in Lyon, and was buried in Lyon Cathedral.
External links
- The Cardinals of the Holy Roman Church - Biographical Dictionary
- Catholic Hierarchy data for this cardinal
|Preceded by
Charles-Martial Allemand-Lavigerie
|Bishop of Nancy
March 27, 1867–March 30, 1882
| Succeeded by
Charles-François Turinaz
|Preceded by
Pierre-Antoine-Justin Paulinier
|Archbishop of Besançon
March 30, 1882–May 26, 1887
| Succeeded by
Arthur-Xavier Ducellier
|Preceded by
Louis-Marie-Joseph-Eusèbe Caverot
|Archbishop of Lyon
May 26, 1887–January 23, 1893
| Succeeded by
Pierre-Hector Coullie
|This article about a Catholic cardinal is a stub. You can help by expanding it.


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Just as HTC announced the Desire Eye and the RE camera last evening, its flagship smartphone from earlier this year, the One M8 is now receiving the Android 4.4.4 update. This also comes with HTC's EYE Experience features which were announced along with the Desire Eye. Smartphones like the One E8, the One Mini 2, the Desire 816, the Desire 820 and many more will receive the same set of features in the coming days.
The EYE Experience software will introduce features like Split Capture, Face Tracking and Face Fusion which were shown off last evening. These features let you use the front and back cameras of the smartphone or combine two images at once accordingly.
The update is over 252MB in size and is rolling out to the unlocked models of the smartphone. As you would imagine, the update will take its time to reach all devices, even more so if you own a carrier variant of the One M8. Already received the update? Make sure you let us know by dropping a line below.
Source: Gizmobolt
Via: Phone Arena


text:
Observing a break in traffic, the gray-haired man hustles across Northwest Washington, D.C.'s Whitehurst Freeway. This man, with his black sport coat and a backpack slung over one shoulder, is now under the freeway's overpass, squeezing his head between a space that reveals someone's elaborate makeshift home. Tarps of different colors cover the rocky ground and suitcases line the walls. A clock, mirror, and Aloe Vera lotion sit on top of one suitcase. On the other side, hundreds of cans and bottles sit unattended.
"Cliff? Melanie? You there?" He calls into the void, but no one answers. He plans to come back tomorrow to check on the homeless couple, but for now, he must move on. There are others to see.
Venturing under highway overpasses, in shelters, and on the streets, Dr. Anthony Martinez searches for homeless people who might need medical attention. Although medical care is available in the nation's capital, many of D.C.'s homeless refuse to go to free clinics and roving medical vans. According to Dr. Martinez, "They just don't trust many doctors. You have to go to them. If you don't go to them, they won't come to you."


text:
The concept of customer self-scanning of grocery orders, which has caught fire in some parts of Europe, continues to simmer at U.S. chains.
Industry observers believe that automated self-checkout systems present a significant opportunity to reduce front-end costs because customers, not paid staff, provide scanning and bagging labor. Even cash tendering can be automated.
In addition, shoppers believe self-checkout time is faster -- even if that perception is actually wrong -- b


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After my sister's wedding the other week, it started to feel like the world had suddenly deflated. The same kind of feeling you get after Christmas day, there had been so much build up to the big event, that afterwards it immediately felt that we needed something to look forward to.
Now I know many of you may or may not remember but my boyfriend and I ventured to Paris to watch the last stretch of the Tour de France last year. So, just after the wedding my boyfriend turns to me and says "Let's drive to Paris", initially I rolled my eyes and brushed it off, but I soon noticed he was serious. Sorry, before I go any further can I quickly paint the picture for you - he passed his driving test on the 9th June and had only been on the motorway the day of the wedding for a couple of junctions. Paris was all of that for much longer and on the other side of the road.... Trying to be the supportive girlfriend I agreed to it, half thinking he'd forget and something else would tickle his fancy (preferably the eurostar!). It serves me right for doubting but on Wednesday morning he announces he's booked the tunnel and sorted accommodation. We were actually going to Paris on Saturday morning. *Glup*
To be fair to him, he drove amazingly. We got over our first hurdle which was starting on the M1 and heading to Folkestone, where we then got onto the tunnel. Our little car Bernie was doing superb and so was my boy!
|Allez Wiggins! Allez Team Sky!
Once we'd got to Calais and after a few domestics we got on the correct road to Paris. This went on for what felt like forever, but we managed to find our campsite for the evening.
We arrived at the campsite quite late so indluged in stone baked pizzas and a cheeky beer before we turned in for the evening. We wanted a good nights sleep (even though I forgot the inflatable mattrass - Oops!), before the long day to cheer on Team Sky.
|The stunning site in Paris!
|Getting the breakfast ready
The campsite was lovely, you would of never known you were nearly in the centre of Paris. You could hire bikes for the day and there was even a shuttle bus into the city centre.
We bought all out camping gear with us, including food bits, and me being me, I bought some of my favourite camping items. I was desperate to try out my Fair Wind Blue Bird Cool Camping Set, this was a fabulous internet find and is ideal for camping!
"This hand-painted camping set is perfect for summer holidays under canvas. It's a great gift for camping enthusiasts that includes a mug, cup and bowl all presented in a simple cotton gift bag. The striking bird design has been hand-painted onto white enamelled stainless steel. This set is also perfect for festivals and glamping, making the great outdoors stylish and fun. This set completely safe for food use, but please gently hand wash only. Bowl: 16.5cm (W) x 16.5cm (D) x 7cm (H), cup: 8cm (W) x 8cm (D) x 12cm (H), mug: 8cm (W) x 8cm (D) x 8cm (H"
|Blue Bird Cool Camping Set - £29.00
It comes in a lovely little gift bag - so you can give it as a gift, or like me be indulgant and keep it for yourself! There are other similar items you can add to your collection like trays and jugs etc. Its very much worth a browse of their website.
After we had enjoyed our food we set out our mission...
|In little Bernie we went - complete with flags!
|We saw Team Sky on the way - gave them a quick hug
|Of course, it'd be rude if I didn't say hello again! ;o)
|In front of the the Arc de Triomphe
Around the champs d'elysees all the action was happening, but unlike last year I noticed more and more Union Jacks. Not that I'm a huge follower of cycling, but was it that because Bradley Wiggins was potentially going to be the first British winner in 70 years - that the British were attracted to it finally? None the less it was a fabulous atmosphere amongst everyone.
|At the Team Sky Fanzone, we met two fantastic guys who had dressed at knights
|Wiggins facemasks
|My boy in his geeky Tour De France hat & moi outside the Arc de Triomphe
It was soon time to head home. The weather had been beautiful, Bradley Wiggins had won and the spontaneous trip was worth every euro. Next year we plan to use a camper van and head out to some of the other hotspots of the Tour De France.
Apparently the weather was fantastic in most places, what did you all do with your weekends? Maybe we can finally all start wearing the summer clothes we've been dreaming of!


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Can any of you recommend a good Cream Cheese Frosting Recipe that I can use to make nice swirls on cupcakes?? t
he ones that I have tried taste really good but the consistency is always too thin and runny...
help please!
if you like the taste but consistency is runny try adding more sugar or refrigerating it a bit to harden it up and then frost.
Another idea is to make it without any liquid.
The first batch of cream cheese frosting I made also turned out very runny. Turns out I had over mixed it.
The next batches came out perfect.
From a terrible wedding cake experience, adding more powdered sugar is not the answer to stiffening up cream cheese icing.
Sugar is hygroscopic, meaning it readily absorbs moisture, so it doesn't have the expected effect of tightening up CC icing.
Usually what I do is use nearly ALL cream cheese, and add only the smallest amount of sugar to make the icing a LITTLE sweeter, a bit of butter for creaminess, and only a tiny amount of vanilla.
Hope this helps...Scott
i love the "crusting cream cheese" recipe in the recipe section here on CC. Has a little bit softer consistency than my decorators buttercream, but it holds it shape and i can use it for borders, flowers, and writing easily.
I have always found that cream cheese icing is predictably unpredictable. One batch can turn out perfect while another (using the exact same recipe) can become a mess! Which is why I won't ice a wedding cake with it. I only use it as a filling.
Here's what I do know that might help you out. Cream cheese usually contains guar gums that break down when overbeaten. So the problem might not be your recipe, just the speed or the amount of time that your mixing it for.
For a nice smooth icing your cream cheese MUST be at room temperature, as does the butter.
I find that most recipes contain the same basic ingredients (cream cheese, butter, conf sugar, vanilla, lemon juice). Most do not contain liquid like milk as it tends to cause the icing to be runny.
I usually first beat my cream cheese on low speed until smooth, then add the butter, beat on low again until combined, then mix in (again on low speed) your conf sugar, vanilla, and lemon juice and your end result should be a nice smooth but spreadable icing.
Another thing I've tired in the past is to mix my butter and conf sugar together with the vanilla, lemon juice and just a touch of milk to a stiff consistency and then beat in the cream cheese until blended.
Hope this helps.
Non-crusting cream cheese icing is tricky. That's why I usually make Edna's crusting cream cheese recipe:
It pipes and swirls beautifully--handles just like a good buttercream! Everyone loves the flavor, and it goes especially well with chocolate, red velvet & carrot cakes, but I've also used it on marble and spice cakes.
As others have said, the cream cheese needs to be room temp for it to properly combine with the other ingredients.
Good luck to you.
ALWAYS use 100% REAL butter, not margerine, or spread, these contain water, which makes the frosting thin. Best of Luck!!!
ALWAYS use 100% REAL butter, not margerine, or spread, these contain water, which makes the frosting thin. Best of Luck!!!
Oh yes, that's an important detail....and the butter should be room temp but not super-melty. This time of year room temp is fine, but in the summer my kitchen is too hot, so I only leave the butter out for an hour or so before mixing the icing.
I've had trouble with my normal cream cheese icing being tempermental, so the last time I decided to experiment. I used 8 oz. of room temp cream cheese to which I added 2-3 cups of indydebi's bc I had on hand (can't remember exactly) along with a tbsp of pineapple juice (it was for a carrot cake that has pineapple) IT WAS DIVINE!!! Best cream cheese icing EVER..not too sweet, but perfectly creamy and creamcheesy Perfectly pipeable, if that is a word. I brought a cake over to my neighbour and they raved about the frosting...apparently they all sat around trying to guess how I made it!


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Can anyone recommend a national Insurance Co that covers the home baker and also instead of using my social security number, would they accept my Tax ID#?
Speaking as someone who worked for CIGNA insurance (property and casualty) for 15 years, AND as a former licensed Life & Health insurance agent .....
Do you own your home? Do you have a car? If so I'm assuming you have an insurance agent? Check with him.
Don't shop for an insurance company on your own. First, most companies don't sell direct to the insured ... they sell thru agents. Second, insurance is contract law and there's a REASON an agent is required to be licensed and know all of those laws. Third, the company I use (and they specialized in food industry coverage) may not write coverage in your state and even if they do, you'd need to find an agent who is licensed in your state AND contracted with this insurance company
Call your agent. He gets paid to shop for insurance coverage and find the best company for what you need.
If you don't have an insurance agent, then find one ... an independent agent is your best bet as they represent a number of companies.
How interesting. I worked for CIGNA for 15 years also, but I was a medical claims processor. Currently I live in an apartment and I have apartment insurance, but awhile back when I had the opportunity to manage/own a coffee shop my agent did not cover commercial insurance. I did have to go the way of an independent. I was just checking if anyone had a specific company they liked. I will check around with the independents. Thanks alot.


text:
Bargain Pawn is a bargain mart/pawn shop featured in Grand Theft Auto: San Andreas.
The store's outlets can be found all around the state of San Andreas. Its slogans are "Everyday Low Prices" and "The Smart Way to Shop". It is inaccessible to the player and plays no role in the storyline whatsoever.


text: As everyone in the startup ecosystem waits to see which way the funding winter winds will turn, Unacademy, the online learning platform, today raised Series A funding of $4.5 million in a round led by Nexus Venture Partners and Blume Ventures. The round also saw participation from Girish Mathrubootham, CEO, Freshdesk, and Ananth Narayanan, CEO, Myntra, along with Stanford Angels. This is the third round of funding for Unacademy; the startup had first raised funding to the tune of $500,000, followed by a funding of $1 million.


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|This article needs additional citations for verification. (June 2012) (Learn how and when to remove this template message)
Barry Newman as Tony Petrocelli.
|Created by
|Harold Buchman
Sidney J. Furie
|Directed by
|Irving J. Moore
|Barry Newman
Susan Howard
Albert Salmi
David Huddleston
|Lalo Schifrin (pilot, 2.2)
|Country of origin
|United States
|No. of seasons
|No. of episodes
|Executive producer(s)
|Edward K. Milkis
Thomas L. Miller
|Leonard Katzman
|Camera setup
|Running time
|48 minutes
|Production company(s)
|Miller-Milkis Productions
Paramount Network Television
|CBS Television Distribution
|Original network
|Original release
|September 11, 1974– March 31, 1976
Petrocelli was an American legal drama which ran for two seasons on NBC from September 11, 1974 to March 31, 1976.[1]
Tony Petrocelli was an Italian-American Harvard-educated lawyer who grew up in South Boston and gave up the big money and frenetic pace of major-metropolitan life to practice in a sleepy city in Arizona called San Remo (filmed in South Tucson, Arizona). He and his wife Maggie lived in a house trailer in the country while waiting for their new home to be built (it was never completed over the course of the series). Tony drove a beat-up old pickup truck, always a little too fast. Petrocelli hired Pete Ritter, a local cowboy, as his investigator.
Petrocelli worked as a defense lawyer, and each episode followed a similar format, with the client apparently certain to be convicted of a crime of which they were innocent until a late-emerging piece of evidence allowed the protagonist to suggest to the jury an alternative possibility. These alternatives were never established as absolute fact, and the trial of the person onto whom Petrocelli turned the accusation never occurred, but the doubt raised was sufficient to secure the release of his client.
A technique used in the TV series was showing the actual crime in flashbacks from the perspective of various people involved. The flashbacks, naturally, differed depending on whose recollections were being shown. To maximize the drama, the prosecution's version was always the first flashback shown (i.e. what supposedly happened), then the client's version was presented (what the client remembered happening), then, finally, after finishing his investigation, Petrocelli presented his version (generally meant to be what in fact occurred). This final flashback always contained elements of the prosecution's and his client's versions, but with his new-found evidence, it would show both the client's innocence and an explanation as to how and why the prosecution and client's versions differed. In other words, neither side was ever meant to be corrupt or lying, rather, without Petrocelli's new information, both previous versions appeared to be accurate from their respective points of view.
Newman created the role of Petrocelli in a 1970 movie, The Lawyer, which was loosely based on the Sam Sheppard murder case. Diana Muldaur co-starred as his wife Maggie in the 1970 feature film. Petrocelli was produced by Leonard Katzman. In the NBC TV series, Susan Howard played the wife of Tony Petrocelli. Howard was later cast as Donna Culver Krebbs in Katzman's prime-time soap opera Dallas.
|Barry Newman
|Anthony J. Petrocelli
|Susan Howard
|Maggie Petrocelli
|Albert Salmi
|Pete Ritter
|David Huddleston
|Lt. John Ponce
Guest stars[edit]
- Anne Archer
- Ned Beatty
- Lucille Benson
- Lynn Borden
- Nancy Criss
- Kim Darby
- Susan Dey
- Harrison Ford
- Ron Foster
- Alan Fudge
- Lynda Day George
- Louis Gossett, Jr.
- Harold Gould
- Mark Hamill
- Robert Hooks
- Julie Kavner
- Sally Kirkland
- Kay Lenz
- Gerald McRaney
- Belinda Montgomery
- Lee Montgomery
- James Naughton
- Annette O'Toole
- Della Reese
- Peter Mark Richman
- Robbie Rist
- John Ritter
- Marion Ross
- John Saxon
- William Shatner
- Loretta Swit
- Joan Van Ark
- Mitch Vogel
- Cindy Williams
- Noble Willingham
- William Windom
DVD releases[edit]
Visual Entertainment released the complete series on DVD in Region 1 on December 16, 2016.[2]
External links[edit]
- Petrocelli on IMDb
- Petrocelli at TV.com
- Petrocelli at epguides.com
- 1970s American television series
- 1974 American television series debuts
- 1976 American television series endings
- American legal television series
- NBC network shows
- Television series by CBS Television Studios
- English-language television programming
- Fictional American people of Italian descent
- Television shows set in Arizona


text: \u0022I won\u0027t wait hours in line for fad foods,\u0022 says the BA Foodist, Andrew Knowlton. Yes, he\u0027s looking at you, cronuts, cronut concretes, and cronut pizza. (\u0022I made that one up, but hey, it can\u0027t be long!\u0022) But classic, last-meal-worthy comfort foods, executed in their most expert, dare we say perfect, forms? We\u0027ll wait for that. For smoky, almost melty brisket; for oysters so clean, cold, and salty-sweet you could call them \u0022crisp\u0022; for the bread we expect to eat in heaven (given we don\u0027t eat so many pillowy doughnuts that we\u0027re sent to that other place). These are the five things our staffers actually would wait hours to purchase, stare at for a bit, and take a couple whiffs before putting in our mouths and closing our eyes, feeling lucky to be alive.


text:
What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
How do I import a previously created form?Asked by sschnare on March 29, 2016 at 12:22 PM
I am selecting CLONE EXISTING FORM ... but the "Please Select" button does not allow me to brows.
I am using both internet explorer and fire fox - won't work in either. Is there a problem?
JotForm Support
I have tested the feature and it is working on my end, please see the sample below:
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text:
Author | exchange4media News Service | Tuesday, Oct 24,2006 6:56 AM
Mountain Dew is undergoing an extreme makeover, scrapping its 13-year-old "Do the Dew" theme and dumping its "extreme" positioning, which many now consider tired.
Frank Cooper, vp-marketing put in charge of the PepsiCo brand less than a year ago, is leading the charge, challenging the company's agencies and consultants to come up with a new plan, per sources.
The move is particularly risky since Dew was the only major PepsiCo soda label to gain market share last year. It also comes as the flagship Pepsi-Cola brand begins its own makeover this week.
Dew's new positioning will be fashioned around "fueling the core." That means "satisfying people's need for excitement or fulfillment in whatever they do," said a Pepsi exec with knowledge of the matter.
Dew will focus on exhilaration, energy and the enjoyment of finding new passions. "Extreme is no longer extreme," said the exec, noting how so many other brands have occupied the niche and taken it mainstream. "We don't know what the answer is. We're waiting for our agencies to come up with something and we're conducting research. We haven't come up with the Holy Grail yet."
The exec said scrapping "Do the Dew," the longest-running campaign in the category, is part of the strategy: "If you change the whole approach, you have to change that. Although we might wink at it."
A Pepsi rep declined to confirm the move. "We're still looking at all of our options. There's nothing to share yet," said the rep. BBDO, New York, Dew's lead agency since 1973, referred calls to Pepsi.
In the first indication of the change in strategy, Pepsi will overhaul Dew's MDX next month. Because of consumer confusion and slow sales, the brand will no longer be positioned as an energy soda. Rather, it will be marketed as a soft drink that is energy-infused to avoid direct competition with Red Bull. "Be Nocturnal" ads will be axed in favor of ads themed "Stay Sharp," which will quiz TV viewers about details from the shows they are watching.
Mountain Dew's marketing, dating to the 1940s, has been so successful it's been the subject of a Harvard case study and at least two books. The lightly carbonated, caffeinated citrus drink embraced an extreme attitude, popular among teens in the 1990s. Dew was one of the first to align with action sports, which distanced the brand from its roots as "zero proof moonshine" when it was derided as "hillbilly piss." Early Dew ads featured guns, outhouses and corny copy like, "It'll tickle yore innards."
The brand now finds itself at a crossroads. It needs to update its image without alienating its core fan base. Skating away from its extreme image makes sense considering "there are people in their fifties doing extreme stuff," said Warren Church, vp-brand strategy at Deskey, Cincinnati. "Extreme sports worked because it was a step outside of the mainstream where you could be an individual. Whatever they do next, it can't be fake."
An agency exec who had previously worked on the Dew brand said it's about time Dew switched strategy: "Everybody is doing extreme drinks. Kids have new passions. They have to take it to the new phase. They just have to figure out what that is."
Dew has had hits and misses lately with action sports, but the Dew Actions Sports Tour will continue. However last year's Dew-sponsored snowboarding film, First Descent, met with poor reviews and ticket sales.
From an advertising perspective, the brand has already evolved from focusing on kids on skateboards in favor of edgy humor that's included everything from dusting off Mad magazine's "Spy vs. Spy" to featuring action star Steven Segal in slapstick comedy.
Dew is the master of making the equivalent of "beer commercials for teens," said the agency exec. Spending for the upcoming campaign was not disclosed. PepsiCo spent $58 million on media for the Dew brand last year and $15 million for the first eight months of 2006, per Nielsen Monitor-Plus. Diet Dew got $25 million through August.
Coca-Cola, meanwhile, spent $21 million through August on Vault, its latest Dew knockoff. Prior attempts, Mello Yello and Surge, failed to make their mark. Dew continues to maintain its spot as the fourth best-selling soda with a 9.5% share behind Coke, Pepsi and Diet Coke, per Beverage Digest, Bedford Hills, N.Y. Vault grabbed a 0.6% share for the first half of the year, putting it on the same scale with minor brands like Barq's root beer.
Coke announced it will launch Vault Red Blitz in March to compete with Mountain Dew's Code Red. MDX grabbed a 0.1% share for the first half of the year.
While the Dew team is hard at work, Pepsi will announce initial plans for its "Feel the Pepsi" campaign this week, per sources. The campaign replaces the three-year-old "It's the cola" campaign.
Source: Brandweek
Programming HeadRadio Mango


text:
|Reviews for A Calm Through the Storm
| krito1389 chapter 1 . 6/30/2014
oowww awesome I loved it!
| Erisa Daelyn chapter 1 . 4/29/2013
Super sweet little ficlet
| Raydiate chapter 1 . 12/15/2010
I disagree wholeheartedly. The beauty of this, however short, one-shot is very easily, in my opinion, the fact that it's not out of character at all. Both Renji and Ichigo seem completely in tune with exactly how they are in the manga/anime and that's what makes the pairing in this piece so believable. It's almost as though, on top of everything they must be dealing with at this stage in their lives, they're still making time for each other. Even in their rather rough conditions. *cough* Renji *cough cough* Anyway, very good job keeping it real and utterly and sweetly believable.
| quixotomy chapter 1 . 5/9/2010
D'aww. It's so cute! I love fluff. :D
| alyuchiha913 chapter 1 . 12/1/2009
Aw! This was so sweet and romantic and just adorable! Yet somehow, not OOC despite all the mushiness. That definitely took some skill. It's great. I love it! The only bad thing I have to say is that you made two spelling errors.
"...the access water dripping from..."
It should be "excess" not "access" and later on in that line you typed "tow" instead of "two" so you should fix that. Other than that, this fic was lovely, and I totally enjoyed it. Good work!
Peace Out! _
| Aurora808 chapter 1 . 8/7/2009
aw!that was so cute but don't tell renji or ichigo i said that please don't
| Aysia-san chapter 1 . 4/13/2009
So very cute and adorable :D
Great job - even if it's only 1,417 words 8D hahah
| SemperSomnium chapter 1 . 3/15/2009
Aww, so cute! I loved it!
| pages.of.altaire chapter 1 . 1/1/2009
so nice! :3 hm...it's not too OOC...I can picture Ichigo and Renji saying and doing those things. :3 *perverted mind*
| D chapter 1 . 11/21/2008
This is without a doubt one of my favourite RenIchi-fics! This was utterly adorable. Thanks for writing!
| AZjanus chapter 1 . 10/11/2008
I could totally picture this happening between them - Renji's single minded focus on Ichigo, Ichigo going from yelling to tender - just a sweet little moment. Nicely written. Thanks for sharing!
| Current Name chapter 1 . 10/10/2008
So cute! I love this! *huggles it*
| narakunohime chapter 1 . 10/10/2008
so cute! :3
| A.Nameless.Traveler chapter 1 . 10/10/2008
naw this is adorable beyond words Ausar!
It makes me wanna hug them both to DEATH. XD
Yay for having inspiration back yes?


text:
Infinitely-repeated games with endogenous discounting
No abstract is available for this item.
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Volume (Year): 52 (1996)
Issue (Month): 2 (August)
Pages: 163-169
|Contact details of provider:
| Web page: http://www.elsevier.com/locate/ecolet
References listed on IDEAS
Please report citation or reference errors to , or , if you are the registered author of the cited work, log in to your RePEc Author Service profile, click on "citations" and make appropriate adjustments.:
- Abreu, Dilip & Milgrom, Paul & Pearce, David, 1991.
"Information and Timing in Repeated Partnerships,"
Econometric Society, vol. 59(6), pages 1713-1733, November.
- David G. Pearce & Dilip Abreu & Paul R. Milgrom, 1988. "Information and Timing in Repeated Partnerships," Cowles Foundation Discussion Papers 875, Cowles Foundation for Research in Economics, Yale University.
- Dilip Abreu & Paul Milgrom & David Pearce, 1997. "Information and timing in repeated partnerships," Levine's Working Paper Archive 636, David K. Levine.
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text:
Veho - Muvi Micro Camcorder - Extreme Sports Pack
Veho Extreme Sports Pack for Muvi DV Camcorders
The Extreme Sports Pack will give you that extra security whether you're cycling down muddy cross country tracks, or skiing down snow covered mountains.
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1 x mounting bracket Velcro
2 x mounting bracket screws
3 x mounting bracket straps
Brand Veho Muvi Weight 0.10 KG Product Number VCC-A001
ReviewsWrite your reviewDelivery Information
In Stock orders placed before 15:00 online, or 14:30 over the phone, Monday-Friday will be dispatched same day. Please choose "Priority" delivery at the checkout for guaranteed next working day delivery or "Standard" for less urgent deliveries. Click for more details.UK Mainland Standard
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If you're not completely happy with your purchase, we'll give you a refund or replacement. Click for more details.


text:
Amazingly enough this beautiful rose is still hanging on, just like the rest of us in this big freeze, taken in November 2010 in my garden
rose frost snow rose in snow morag bates winter ice
Amazing and so beautiful…… it is all about the "hanging on" all through life!!! You have a strong beauty here…. good for you!!!!!! Stay safe and warm, Morag!!!!Brenda
A Beautiful rose covered in snow and Ice.!
So enticing and beautiful with snow kissing the rose beautiful always with love Normajean.
Oh, how extremely lovely! It's just frozen in time! Love it!
Lovely work! :)
Beautiful shot..
This looks so beautiful, Morag. Beautiful composition and colour.
Wonderful creation Morag - avesome – instant FAV.GreetingsJens.
How lovely!
Beautiful capture, Morag!!
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text:
The popular rock bolt manufacturer for your choice-Sinorock
Sinorock is the popular rock bolt manufacturer in China.Our mission is to be the preferred supplier of geo-support solutions and products in the world.We exist to develop and provide innovative geo-support products and solutions with exceptional service and lasting quality.
Rock bolts is beneficial for slope stabilization and also used for load transfer from soft strata to hard strata. As far as safety aspect concern it gives better results because of its stabilization and homogeneity between all rocks.Sinorock rock bolt can be used as a choice for pre-tensioned anchors in loose or collapsing soils without the need for a casing. A free length must be installed onto the anchor if the project specifications call for a pretension load to be applied from the bond length.
Sinorock consistently meets our customers needs for rapid turnaround and delivery. Please take a moment to browse the wide range of quality products we have to offer. If you are not finding what you are looking for, please contact our friendly and thoroughly trained sales staff to answer any questions you may have with rock bolt, we can make it, import it or locate it for you.


text:
Can you cut out fondant with any of the new fangled die cutting machines like the cricuit or the sizzix machine at costco? I don't know if any of these can feed in fondant.....sounds like a great idea! anyone have any ideas?
I have heard that you can but have not tried it yet. My Sizzix just arrived 2 days ago, so I haven't had a chance to try it. My thought, however, when looking at the dies themselves, I wonder if the fondant would get into the foamy part of the die and contaminate it? I was thinking if any bits from the fondant got in there, it would be really tough to get them out.
I cut a piece of fun foam yesterday and pieces of the black foam part of the die we left stuck around the cut out shape. That leads me to believe that the fondant would then not be safe to eat either.
I'm interested to hear of other people's experiences with the Sizzix.
I would think the Cricut would be a better choice as it uses a blade (I believe) and you could have a blade that you use just for the fondant.
theres 2 huge threads on using the cricut for cutting fondant and gumpaste
the cricut is on my never ending cake tools wishlist
here are the links


text:
Unfortunately, after a year or so of reliable service Spiceworks seems to be becoming less and less reliable and we now have a number of problems with it. I'm really hoping folks here can help!
- Continuous scanning loops. The scans never complete and run continuously. They chew up considerable resources on the server and cause a considerable slow down, particularly within Spiceworks itself.
- Totally inaccurate reporting of offline devices. Long lists of items considered to be offline by Spiceworks yet the devices are not offline and do respond to pings. This has even included devices such as my own workstation (whilst I have been working). The information provided is so inaccurate as to be unusable.
- Periodic and seemingly random re-classification of certain devices.
- One particular range of IP addresses keeps generating phantom entries.
- Totally confused interrogation of some SNMP devices, particularly managed switches. The information reported is so inaccurate that it is unusable.
For background information we were running v5.0.62122 on a Windows Server 2008R2 platform. The server is a Dell PowerEdge T105 with a dual core AMD Opteron 1212 processor and 8GB of RAM. There are a couple of other IT programs which run off the server but these are dormant much of the time meaning Spiceworks has pretty much all its resources to itself.
We tried restarting the Spiceworks service but it had no effect. We found that a full reboot of the server caused the scans to return to normal for a period of about 24 hours but then the continuous loop started again. It did nothing to cure the other problems.
Eventually we uninstalled Spiceworks and wiped out every trace of it we could find and installed v5.0.62863 from scratch and restored the database. Since then performance has improved hugely and the program works much faster. However as of this morning the scanning loops are back (though the performance has not yet deteriorated that much). The offline devices list is much more accurate but yet still contains errors. The inventory is currently looking okay, though it did add back in these phantom devices again.
So the main two problems are:
- Why do the continuous scanning loops keep returning? It is set to do a full scan every 6 hours and run health checks on the default interval.
- We have installed a load of new pieces of network kit recently. Spiceworks detects the switches via SNMP and is able to determine devices connected to particular ports - though when on tests this is just one port linking the new box into the network. Once the box is deployed obviously live equipment is then plugged into it meaning the boxes generally have devices connected to some or all of its ports (up to 48). However in Spiceworks this information never updates. For instance I have a stack of 2 x 24 port switches in my network SEG2, each with all ports in use. Spiceworks however shows one switch as uplinked via port 23 (as it was when on test) and all the other ports as out of use. However the configuration of the device (and the SNMP scan) are unchanged.
Any help much appreciated because I am coming under pressure to remove Spiceworks from the organisation as unfit for purpose but I am not ready to give up yet.
This topic was created during version 5.0.
The latest version is 7.5.00101.
3 Replies
Apr 4, 2011 at 3:42 UTC
In case you haven't read through this, the comments regarding the ping_external setting and the sweep_ping setting in the Phantom Devices thread (http:/
Apr 4, 2011 at 5:25 UTC
I have sent you a PM on this issue. Please check it out when you get a chance.
Thank you!
Apr 5, 2011 at 4:37 UTC
Tim, thanks I have changed to ping external and a scan is currently taking place. I'll see if it makes any difference.
James, I have the PM and will follow the instructions as soon as I am able. Many thanks.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
The Southern tip of the Nicoya Peninsula in Costa Rica feels like another world compared to the hustle and bustle of a town like Tamarindo, which is located just a few hours north. Nicoya Peninsula is known for its rolling green hills and lush surroundings thanks to the abundance of rivers and underground water streams in the area.
It's not an area frequented by tourists (yet), but over the years, the towns of Santa Teresa, Montezuma, and Mal Pais have all welcomed their fair share of expats. At first, it was mostly avid surfers, who needed very little to enjoy life on the shores of the Pacific Ocean. Then followed the backpackers who played a big role in placing Santa Teresa on the map as an off the beaten path destination in Costa Rica. With backpackers came hostels, b&bs, restaurants, and shops and just like that a little fishing village flourished into a destination that visitors now struggle to say goodbye to.
But don't let that fool you. While many love the fact that Santa Teresa managed to maintain its rough around the edges feel, we feel like it's our responsibility to tell you that it's not a town for everyone.
Don't get us wrong, we enjoyed our time in Santa Teresa, but without a board in hand we struggled to find our place in town or a good reason to recommend Santa Teresa to everyone visiting Costa Rica.
The main beaches in town, Playa Carmen, and Playa Santa Teresa are lovely. They are long and wide, but they aren't the best swimming beaches in the country. Rough seas and ample of surfers in the water made it hard for us to get out for a swim. We enjoyed spending the afternoon catching some sun rays and walking along the beach, but all in all this area didn't offer the best beach experience in the country.
If you are a surfer or someone who wants to take up surfing, you'll love Santa Teresa! This region is known for different beaks and a constant swell all year around. Most hotels and shops dotted along the main road in town offer surfboard rentals and surfing lessons.
Santa Teresa is also home to one of the most legendary surf breaks in Costa Rica known as "Suck Rock" located in front of the Red Palm Villas Resort. Next to Suck Rock there is "La Lora", known as one of the most consistent beach breaks.
Max debated grabbing a board and catching a few waves but since I couldn't join him in the water after my surfing accident earlier this year, he decided against it.
There are lots of different activities offered in Santa Teresa. You'll find everything from yoga and cooking classes to horseback riding and ATV tours. A lot of these can be booked at Ollie's Adventures or at a number of other shops in town. But if you are looking for something unique to do in Santa Teresa, an activity that's not available elsewhere in Costa Rica, you be hard pressed to find something.
Eating was by far our favourite pastime in Santa Teresa. When it comes to culinary delights this town is small but mighty. Our favourite spot was Habanero, a Mexican Grill and Bar with amazing food and delicious drinks, located on the beach at Playa Cielo. We took advantage of their 2 for 1 happy hour and indulged in some unique cocktails (tamarind-ginger and mango jalapeño margaritas…yum!) while enjoying a beautiful sunset.
We also really enjoyed our meal at the Argentinian joint, Las Piedras. The restaurant's main draw is their open air BBQ grill that they used to cook up our incredible meat platter.
Burger Rancho was another one of our favourites, offering a great selection of gluten free dishes, burgers, and seafood specialties.
For breakfast, we opted to eat at a local soda, because no matter where in Costa Rica we are, we can never say no to a great traditional breakfast of Gallo Pinto, eggs, and fried plantains. If you are looking for a more American choice The Bakery across from Playa Carmen and Zwart Cafe offered some great, although slightly overpriced, options.
We'll admit we were a bit underwhelmed by Santa Teresa. We expected a bit more. More nightlife (there was none), better swimming beaches (we eventually found one in Montezuma), more unique activities, and things that aren't offered in every other Costa Rican tourist hub.
Should you add Santa Teresa to your Costa Rican itinerary? We'd say do it if you have the time. Come for surfing or surf watching and expect to spend your days relaxing. Be prepared to brave the horrific roads in town, and come to enjoy the real Pura Vida lifestyle.
If you are only in Costa Rica for 1-2 weeks and surfing isn't high on your to-do list, skip it and spend your precious days exploring towns that offer more than paradise for surf lovers!
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What are draws you to new destinations? Is it activities, great restaurants, shopping, or something else? We'd love to hear your thoughts in the comments section below