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Son 14
- Overview
- Biography
- Songs
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- Similar Artists
|1990s 2000s
Formed in Santiago, Cuba, in 1977 by vocalist Eduardo El Tiburon Morales (1935, Cuba) and pianist/musical director Adalberto Alvarez, Son 14, as their name would suggest, are a 14-piece band playing son, Cubas national musical style. In 1978 Lazaro Rosabal (b. 1953, Santiago, Cuba) joined the group. They recorded a number of albums for the local EGREM label, many of which became bestsellers throughout Latin America. The band also toured extensively across America and Europe. In 1992 co-founder Alvarez left the band to form his own group. Undaunted, Son 14 continued, with Rosabal taking over as musical director. As well as continued success within the Latin market, the band also began to gain recognition internationally, firstly with the song El Son De La Madrugada which featured on the Earthworks Records compilation Cuba - Fully Charged (1993), and then with the release of Son - The BIG SOUND (1995), a compilation of the...


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I making a wedding cake for next Sunday and the bride wants strawberry mouse filling and wants to add chopped almonds and fresh strawberries. How far in advance can I make the mousse filling for Sunday's cake?
With the fresh fruit shes added I know I have to keep the cake in the frig, but once delivered to the reception does the cake need to go back in the frig before they cut the cake?
Has anyone added nuts to a mousse filling before? Just wondering how it will taste? Will it change the flavor of the mousse?
The mousse will be just fine w/nuts added. Chop them rather well. It won't change the flavor that much.
Fresh berries will cause it to be very runny
Don't worry about the filling once you deliver the cake. The filling is sealed in. The only problem would come if any leftover cake is not chilled for days.
It also depends on where you are.......is it hot and humid? That would cause problems but most of us are in winter with chilly temps
It's winter where I live right now.
oh boy I wasn't expecting for the strawberries to make it runny. So fresh Strawberries I guess is out of the questions. Uh?
I was thinking of covering the cake with ganache and then covering the ganache with butter cream. (don't know if this came be done or not) But my thinking was if I covered the ganache I will be able to seal the filling very well and then use the buttercream over the ganache to decorate. I am planning to decorate with a template around the cake.

subset: commoncrawl


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A solid wood extending dining table that can extend from 90cm to 130cm when needed
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text: Numbuh 4 in FusionFallWallabee in the opening credits.Numbuh 4 disguised as Numbuh 1Numbuh 4 in Operation: C.A.K.E.D. .F.I.V.EIf you look closer, he's smiling at Kuki!Numbuh 4 as a Spank-Happy VampireSector V scolding at Tommy for framing Chad that he did to that bike rack this morning.Numbuh 3 and 4 are about to be run over by the Tommymobile.Add a photo to this gallery Retrieved from "http://knd.wikia.com/wiki/Numbuh_4/Gallery?oldid=67674" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Gallery


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February quick post! Today the game site [www.gameinformer.com GameInformer.com] informed us that the third number game of the AC trilogy now has a release date: Assassin's Creed III will come out on October 30th, 2012. Aside from that, Ubisoft's CEO Yves Guillemot said:
- "We will push the title a lot because it's a fantastic product that the team has been working for three years, what we have seen is just fabulous."
No more details were given.
Besides from that, I am saddened to announce the resignation of one of our oldest Istruttore, User:Assassin-Rayne. Personal life has catch up with him and he could no longer fulfil his duties for the past months, and as such had to leave the wiki staff. He will surely be missed.
Ah, one more thing: User:Slate Vesper asked me to mention his blog about the new ACR achievements and what seems a hint at a DLC, so here it is: User blog:Slate Vesper/ACR Story DLC hinted at?
There you go, mention made, Slate -- I expect my payment on costumary chocolates and dragon plushies.
This is all for now, brothers and sisters. Stay tuned for more AC news and sound off on the comments below. =]
Safety and peace, folks.
Cello out.
"If you can find something everyone agrees on, it's wrong."


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Cogs are the Machine Empire's highly sophisticated fighting robots. These metal monsters do not know the meaning of the word surrender. In order to defeat them they must be shorted out or dismantled. The Cogs can fire energy blasts from their eyes, and can also fight using a short baton with spikes on either end.
| Some or all of the information and/or categories on this page may have come from another site such as the Villains Wikia or TVTropes.org. This may include previous edits that are different than the current version. Changes to this page to provide original content are welcomed and encouraged, but this notice must remain on the page at all times.
To visit this page on the Villains Wikia, click here.


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Entrepreneurship at the University of Michigan School of Information is broadly defined and applicable to students in all specializations and degree programs. In part, it's about how you think about and solve problems and how you can use that knowledge in all that you do, in all of your classes.
UMSI Entrepreneurship Program
The UMSI Entrepreneurship Program was founded in 2014 and is led by Nancy Benovich Gilby, Ehrenberg Director of Entrepreneurship. We are a community that empowers students to develop their entrepreneurial skills to turn ideas into impact. We are united by our passion for the fundamental intertwining of people information and technology, and strengthened by our diverse backgrounds. We create learning experiences for students to explore entrepreneurship in ways that they find relevant, and inspire creativity, confidence, and resilience.
Building skills, bringing them back
The program sponsors annual Startup Treks to visit major innovation centers across the country. Students get the opportunity to network, receive mentorship from successful leaders, and build their own entrepreneurial skills.
Passion-led projects
We believe that the best way to develop entrepreneurial skills is to apply them to something you're passionate about. That's why we've made it our big hairy audacious goal (BHAG) to inspire and support every student to participate in at least one passion-led, self-driven innovation project during their tenure at UMSI.


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Armando Geller
Armando Geller
George Mason University and Scensei
Armando Geller is a computational social scientist and co-founder of Scensei, a decision support and analytics enterprise. He is also affiliated with the School of Conflict Analysis and Resolution at George Mason University. His specialization is in information elicitation in challenging circumstances and evidence-driven model design. Sometimes he lectures on computational social science and contemporary conflict.


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It only takes a minute to get started. Fill out our uncomplicated sign up form and login to the website.
This step is voluntary but will greatly raise your chances of getting interest from other over 50s members on the site. Tell people about yourself, your hobbies and attach any nice photographs of yourself.
Use our website's sophisticated search to encounter over 50s members who match your desired requirements. Or just browse through listings and encounter singles that you find attractive.
Found someone who looks suitable? Send them a few words or just a wink and see what they say. With a little luck you'll be on your way to an amazing adventure.
It only takes a few clicks


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
How to create a chatting system on my Weebly siteAsked by mirandascontent77 on May 24, 2016 at 02:11 AM
i want a feedback page where people are chatting and leaving comments for one another and then replying back at a later time even if the person is not around, so that way they can chat back and forth between one another and help each other with what they have learned, shared, their own personal experience, and give advice, feedback, susggestions, reply w/ their own story etc, so that way there's a communication page i have where people r helping ea other on that specific page that i build? can u assist me cuz it needs to be on a page that i create even though i am not finished w/ the page. thanks but will go head n leave a link to what pg i do have published. thanks so much!
JotForm is an online form builder service, which helps you build forms easily. It is not the best solution if you are looking for a chatting functionality.
However, you can implement a kind of rudimentary chatting system by using our tools, though it will not be like an actual chatting platform. What you can do is to (1) have a feedback / messaging form that your users will be submitting.
(2) In order for other users to see what users before them have submitted, you can also create a grid report of all your form's submissions, and then embed it onto your site above the messaging form:
This will allow all users to see all the messages other people have submitted through the form.
If you are looking to have an actual chatting system, with direct or private messages, you may need to use a chatting service instead of our forms. I've found the following recommendations for creating a chatting system on your website:
I hope this helps with creating the chatting system for your visitors / users.


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Jan and Kelly, Day Hiking Ambassadors
It is true, that born in the 60's, I fall back on flower children expressions when describing my snowshoeing experiences. The theme of peace and love were made popular during my era and are truly timeless for a mountain girl like me. Getting out on the trail conjures up … Read More
Jan, Day Hiking Ambassador
If you snowshoe often, it's a good idea to take care of your muscles and stretch before or after your outing. I wrote in last month's blog , about taking up a yoga practice for a balance of the high energy of snowshoeing activity. Yoga poses also serve as my stretch of … Read More
Jan, Day Hiking Ambassador
Snowshoeing is gaining popularity as a winter activity that can increase your fitness level and is a great way to get or stay in shape during the winter months. Since this activity also works the heart muscle, it is considered aerobic and is an excellent form of low impact exercise. There aren't … Read More
Jan & Kelly, Day Hiking Ambassadors
As the high peaks of Colorado are receiving their first season's measurable snow, our efforts turn to trail planning and gathering gear for another exciting year of snowshoeing. Team Jan & Kelly are working up an appetite for our first taste of snow.
It's been an eventful hiking season and we … Read More
Everyone has a favorite trail. The trail where you can expect what's around the next turn, and ends with a familiar view. What's wonderful about revisiting your favorite trail throughout the year, is to watch the landscape change with the seasons.
Our Ambassadors enjoy hiking the same trail in both summer and winter. Scroll through the … Read More
Jan, Day Hiking Ambassador
Snowshoeing for us, has always been about getting away from the crowds, finding peace in nature and keeping fit during the winter months. As this season draws to a close, my friends asked me to do a snowshoe race at one of my favorite resorts, Beaver Creek, which is nestled in … Read More
Jan, Day Hiking Ambassador
Romp to Stomp is more than Tubbs Snowshoes' signature fundraising event for breast cancer. It's about relationships. Connections with our mothers, daughters, sisters, brothers … family and friends and those you don't know, but feel akin to. This event is an opportunity to gather, create memories and celebrate life, survival and … Read More
Jan, Day Hiking Ambassador
Last season I started posting our snowshoeing adventures to Instagram, and have attracted many followers who want to be part of the excitement created by the Colorado snowshoe experience. We have enjoyed making connections with several like-minded, adventure driven people this season and what we have learned is that making snowshoeing … Read More
Jan, Day Hiking Ambassador
Posting photos from our snow shoe trips has attracted family from all over the country to reach out to us. They see the stunning places snowshoes have taken us and are inspired to experience the snowy mountain trails for themselves. We really enjoy planning places to explore and taking others with … Read More
Jan & Kelly, Day Hiking Ambassadors
When you have the opportunity to take friends or family on their first time snowshoe adventure, it is an opportunity to help make their experience an enjoyable memory.
Throw in a fun activity to keep the mood light and fun. This outing, we decorated trees for the holidays with festive … Read More


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Canada's premiers (the leaders of provincial governments),
if they have any breath left after complaining about Ottawa
at their late July annual meeting, might spare a moment to do something,
together, to reduce health-care costs.
A 16-month investigation into a deadly rail crash
in California points to a commuter train engineer's text messaging
as the primary cause rather than malfunctioning signs along the track,
safety officials indicated Thursday
How would it be possible for Cohen at the ripe-old skating age of 25
to do what she couldn't do as a teenager, when she was in her prime?
It's my all time favourite recording of that song,
because you're hearing primitive Baptist music straight from the soul.
Nescafé has found an avid market there for coffee with collagen,
a protein found in connective tissues, on the theory that ingesting
it will improve skin.
Some experts argue, however, that these huge multiple births
occur precisely because U.S. health-care programs and insurance
do not cover such treatments, allowing them to be defined
by the free market of patient choice.
The splendid Dante Quartet capture beautifully
both the shifting light on Debussy's impressionist canvas
and the luminous filigree of Ravel's transcendent creation.


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Photos are for members only!!
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Escort ads, casual sex groups, personal ads, video chat and much more.
I would like alot of thing's . But mainly sum unadulterated fun what can I say other than I can do a bunch of different tricks.


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NEW YORK (Reuters) - The U.S. Federal Reserve's decision on Wednesday to raise rates by a quarter point and to signal a faster pace of increases in 2017 was reasonable, according to leading bond investor Jeffrey Gundlach, given growing inflationary pressures and U.S. President-elect Donald Trump's expected stimulus plans.
"The Fed did the right thing," Gundlach, the chief executive of DoubleLine Capital, said in a telephone interview. "They had to change something because of the inflation indicators. You cannot say, 'We are going to dust off the statement from March'."
Gundlach, who oversees $106 billion at Los Angeles-based DoubleLine, said the bond market was caught "off guard" after the Federal Open Market Committee said it was expecting to raise rates three times in 2017, an increase from the Federal Reserve's September meeting at which the committee said it foresaw two increases.
"The guidance is that the Fed could go three times next year - that is the big news," Gundlach said. The U.S. central bank could raise interest rates three times in 2017 "if the Consumer Price Index headline prints above 3 percent," he added.
Shorter-dated U.S. Treasury yields rose to their highest point in more than five years.
Yields on two-year Treasury US2YT=RR notes rose to their highest level since August 2009, while three-year yields US3YT=RR hit their highest since May 2010 and five-year yields US5YT=RR rose to their highest since May 2011.
Gundlach, known on Wall Street as the "Bond King," went "maximum negative" on Treasuries on July 6 when the yield on the benchmark 10-year Treasury note hit 1.32 percent. On Wednesday, the yield on the 10-year Treasury note closed around 2.57 percent.
U.S. stocks fell the most in two months following the Fed's decision, which Gundlach blamed on the market being "overbought."
"It went up massively and now it is coming down from those levels," he said.
Gundlach told Reuters on Tuesday that a U.S. 10-year Treasury note yield above 3 percent will harm the stock-market rally and housing market.
"I think above 3 percent is a problem," he said On Tuesday. "If the 10-year goes above 3 percent, you would also have to say unequivocally you have seen the end of the bond bull market."
Reporting By Jennifer Ablan; Editing by Bill Rigby


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|Use of thiazolidinedione derivatives and related antihyperglycemic agents in the treatment of impaired glucose tolerance in order to prevent or delay the onset of noninsulin-dependent diabetes mellitus
|Novel methods of using thiazolidinone derivatives and related antihyperglycemic agents to treat populations experiencing impaired glucose intolerance in order to prevent or delay the onset of noninsulin-dependent diabetes mellitus (NIDDM) and complications arising therefrom are disclosed.
|Olefsky; Jerrold (Solano Beach, CA), Antonucci; Tammy (Mequon, WI), Lockwood; Dean (Ann Arbor, MI), Norris; Rebecca (Kewadin, MI)
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|Accueil | Aperçu | Revues | Soumettre | Nous Contacter | English
The recent development of analytic high-throughput technologies enables us to take a bird's view of how metabolism is regulated in real time. We have known for a long time that metabolism is highly regulated at all levels, including transcriptional, posttranslational and allosteric controls. Flux through a metabolic or signaling pathway is determined by the activity of its individual components. Fluxomics aims to define the genes involved in regulation by following the flux. Two technologies are used to monitor fluxes. Pulse labeling of the organism or cell with a tracer, such as 13C, followed by mass spectrometric analysis of the partitioning of label into different compounds provides an efficient tool to study flux and to compare the effect of mutations on flux. The second approach is based on the use of flux sensors, proteins that respond with a conformational change to ligand binding. Fluorescence resonance energy transfer (FRET) detects the conformational change and serves as a proxy for ligand concentration. In contrast to the mass spectrometry assays, FRET nanosensors monitor only a single compound. Both methods provide high time resolution. The major advantages of FRET nanosensors are that they yield data with cellular and subcellular resolution and the method is minimally invasive.
Cells and organisms dynamically acclimate their metabolism to changing conditions, such as nutrient availability, temperature or stress. Flux across the plasma membrane and through metabolic pathways is continuously optimized. Sensory systems that are coupled with complex signaling networks adjust the flux and its direction via regulatory circuits. The sensory systems measure the extracellular availability of a given metabolite and other external cues, as well as the intracellular level of the metabolite or subsequent intermediates, and send signals to regulate transporter and enzyme activities by posttranslational modification, protein turnover, or changes in the rate of their biosynthesis. At present, we know little about the receptors that detect the signals, the signaling networks that transmit the information, nor their integration. A new discipline, fluxomics (Box 1), aims to systematically analyze the fluxes occurring within a cell, and at some point even to unravel these networks in a multicellular organism. The availability of large mutant collections, RNA interference (RNAi) or overexpression line libraries, and large chemical libraries now puts us in a position to unravel these networks, provided we have the tools to measure metabolite flux, especially in high throughput. This review discusses two sets of methodologies that have been developed to measure flux: first, mass spectrometric analysis of changes in metabolite levels after pulse labeling, and second, quantitative imaging using fluorescence resonance energy transfer (FRET)-based nanosensors. These recent developments are contrasted to established isotope labeling techniques for metabolic steady-state systems.
All kinds of physical systems — living cells are no exception — are governed by a dualism between potentials and flows. Potential quantities are related to energy levels whereas flow quantities are related to transport or conversion phenomena. In mechanics, potentials and flows are represented by potential energy and force (a force is a flow of momentum), in hydraulics by pressure and liquid flow, and in electrical systems by voltage and current. For example, voltage can be directly measured by using electrostatic forces, whereas current, that is the flux of electrons, can be measured using a light bulb: the number of collisions per time unit determines the intensity of light emitted. In biological networks, the dualism is given by chemical activities and reaction rates, also called metabolic fluxes. At present, the potential, for example the concentration gradient, can be determined but fluxes must be derived. The precise relation between potentials and flows is described by constitutive laws such as Hooke's law in mechanics, Ohm's law for electrical systems, Fick's law for diffusion, the Nernst equation for electrochemistry, or mass action kinetics in chemistry. However, these are just the simplest examples.
The dualism between substance concentrations, or more precisely chemical potentials, and metabolic fluxes is expressed by the fact that one part is causal for the other. On the one hand, chemical potentials of reactants constitute the driving forces for fluxes. On the other hand, metabolic fluxes change the potentials of metabolite pools. Usually, flows in physical systems are closely related to system functions (i.e. dynamics), whereas potentials rather describe their stock-keeping aspects (i.e. statics). In the field of biochemical networks, in particular, metabolic fluxes are the ultimate manifestation of the cell's function under certain physiological conditions.
It might appear surprising that flux, such as that across the plasma membrane, is not optimized for a wide spectrum of conditions. The plasma membrane provides a limited compartment [1], however, and a given transporter (at least if not modified), has defined kinetic properties, such as high affinity (typically coupled with low capacity) optimized for importing nutrients present in low abundance or high capacity (typically coupled with low affinity). Thus, when nutrient availability or demand change, either a different set of transporters is required or the properties of the transporter have to be adjusted to allow for optimal flux [2–4]. The same is essentially true for many isozymes. It is thus important to determine both the flux potential and the actual flow, and to compare the system's behavior under different conditions, for example, different levels of nutrient availability.
In recent years, fluxome analysis under steady-state conditions has become a widely used tool. It is applied to characterize an organism [5,6], to diagnose the effect of genetic manipulations [7], to compare the behavior of one organism under different physiological conditions [8], to detect the presence of certain metabolic pathways [9,10], to compare mutant libraries [11••], or to monitor different growth phases [12]. This tool has been applied to all classes of organisms, including bacteria, unicellular eukaryotes, animal and plant cells, and even whole organs. Some recent reviews overlook the general methodology at the state of the art [13•,14–17]. Particular problems of plant fluxome analysis have been addressed by Ratcliffe and Shachar-Hill [18] and by Schwender et al. [19], and its application in plants is exemplified in a number of papers [20–22].
Fluxes are usually determined under metabolic steady-state conditions. There is, however, no reason why fluxomics should be restricted to this case. In fact, the rapid redirection of fluxes under dynamic conditions is an important capability of living organisms. Consequently, a new aspect in fluxomics is the consideration of time-dependent fluxes. As we discover below, the experimental methods required to determine these fluxes are quite different from steady-state methods. Interestingly, some current developments are combining methods from dynamic and steady-state approaches to reach new ambitious goals.
Although, the description of living cells by fluxes seems to be more natural than a description by substance concentrations, cells have predominantly been characterized by their intracellular pool sizes. The reason is rather simple: direct measurements of flows are possible in other physical systems but there is almost no known measurement procedure that directly yields information on metabolic reaction rates (an exotic exception might be the light emission in the luciferase reaction [Figure 1a]). This makes fluxomics a rather singular discipline in the 'omics' field.
As an example that illustrates this difference, electrical currents can be measured not only directly by magnetic induction but also indirectly from the voltage change of a capacitor. In the latter case, the signal must be differentiated with respect to time to obtain the time-dependent current. For an electrical system, this is usually not crucial because the measured signal has a high precision. This situation is completely different in the biological case, where measurement noise is significant and a direct differentiation leads to error amplification. To solve this problem, only initial velocities are calculated or signal smoothing algorithms are applied (Figure 1b). However, this helps only in case of a high data density or slowly changing pool sizes. If both approaches are not applicable, a model-based data evaluation is preferred in which a constitutive law is assumed (e.g. Michaelis–Menten kinetics) and only the parameters of this law are estimated (Figure 1c). This strongly reduces the required amount of measured data, but the interpretation of the data is 'prejudiced' by the assumed law.
The present review focuses on the comparison and classification of different fluxomics methods for dynamic and static conditions and their interrelations. The flow-potential viewpoint will serve as a guideline. As the dual quantity of fluxes is given by the metabolite pool sizes, methods from metabolomics play an important role here by supplying the raw data for flux determination.
The classical idea of measuring enzyme kinetic data from initial slopes of NAD signals immediately leads to indirect flux analysis methods that are based on time-resolved pool-size signals. A well-known class of experiments to investigate metabolic networks under highly dynamic conditions are represented by stimulus–response experiments ([23•,24; Figure 2a). Here, an external stimulus is imposed on the system by suddenly raising the extracellular concentration of a substance at time zero. The intracellular pathways immediately respond to the stimulus, usually within seconds or minutes. Under these highly dynamic conditions, the most difficult analytical problem is clearly the reliable quantitative measurement of intracellular pool sizes within short time intervals and with minimal interference with cellular functions.
Two methods have been developed to obtain dynamic flux information from measurements of pool size. In the first, a pulse stimulus in combination with rapid sampling serves to draw samples from a cell culture at high sampling frequency, up to several samples per second [23•,25]. Subsequently, each sample is rapidly inactivated, for example by cold methanol quenching. Cell disruption and separating the intercellular metabolites allows pool sizes to be determined using modern high performance liquid chromatography (LC)-MS instruments [26]. In essence, this is a technical improvement over the method originally introduced to biology by Calvin and Benson [27] to determine the carbon path in photosynthesis. The reliability of this procedure is still a controversy, however, because of the leakage of cell membranes when using methanol quenching. Moreover, it does not provide spatial information when applied to tissues or organs. Neither does it provide subcellular spatial resolution, which is of primary relevance in eukaryotic systems due to their high level of compartmentation. Finally, because of the destructive nature of this approach, the analysis of tissues is limited to parallel sampling. Thus, to date, fluxomics had been restricted to the analysis of unicellular systems in the population average.
An exciting new alternative to this method is given by the direct expression of sensors for the concentration of molecular metabolites in the cell. Certain proteins, specifically chemoreceptors, respond to ligand binding with a conformational change [28]. Protein conformation can be measured directly, using fluorescence resonance energy transfer [29]. FRET reporters in which recognition elements from diverse bacterial chemoreceptors are combined with green fluorescent protein (GFP) variants have permitted the development of genetically encoded flux-sensors for a variety of small molecules, such as calcium, phosphate, carbohydrates (ribose, glucose, maltose and sucrose) and amino acids [30,31••,32•,33•,34,35]. These sensors can detect flux changes in vivo because they monitor steady-state levels of a given ion or metabolite.
A single sensor can already be used to analyze large mutant collections from any organism. The major advantage of these sensors over any other technology lies in the time resolution that they can provide (up to millisecond range as shown for calcium [36,37]). These sensors also provide high spatial resolution; for example, they have successfully been applied to analyze metabolite levels in individual cells within intact organs [38•,39,40••]. On the one hand, a single sensor is able to monitor fewer substances than a MS approach.
Since these FRET sensors are genetically encoded, they provide subcellular resolution. This subcellular resolution is not achieved by optical methods, but genetically by targeting. The addition of targeting signals has successfully been used to monitor glucose flux across the membrane of the endoplasmic reticulum (ER) or nuclear fluxes [31••,41. Thus, local fluxes can be monitored by anchoring the sensors in specific membranes, for example at the cell surface [32••], and it is conceivable that even flux in localized domains, such as rafts, can be monitored this way. Finally, and most importantly, the sensor approach is non-destructive, making it possible to monitor an intact organ (or organism) with minimal invasion. Obviously, the expression of the sensors with a cell adds a new buffer for the analyte of interest that may affect metabolic flux.
The computer evaluation of dynamic experiments to estimate the time-dependent intracellular fluxes between the metabolite pools must currently rely on the assumption of a reaction kinetic mechanism (i.e. constitutive laws) for all of the involved transport and reaction steps. This gives rise to a differential equation model that describes the dynamics of intracellular pool sizes. By fitting this model to the measured data, the in vivo parameters of the enzyme kinetics are estimated ([42•]; Figure 3). Finally, the time-dependent metabolic fluxes (as a function of time) can be directly computed from the fitted model [43,44•].
13C metabolic flux analysis (13C-MFA) is currently the best-established fluxomics technology. In contrast to flux analysis under highly dynamic conditions, the 13C-MFA method always requires the assumption of metabolic steady state for the entire duration of the experiment. This limits the possible applications, but on the other hand, no assumptions on reaction kinetics are needed to derive the fluxes from the available measurements. Clearly, in this situation, knowledge of the intracellular metabolite pool sizes does not help to determine the fluxes.
This major source of information for computing the fluxes is the isotope labeling of substrates that are fed into the system (usually with 13C but other isotopes are discussed [45•]). After switching the feed to a labeled substrate, the isotopes are distributed over the intercellular network by metabolic activities (Figure 2b). After some time, both the metabolic fluxes and the fluxes and fractions of labeled material in the metabolite pools can be shown to have reached a steady state [44•]. This fractional information can be obtained using NMR or MS. Interestingly, once again, the intracellular fluxes have to be determined from a concentration-like quantity.
The measured fractions of isotopic labeling in intracellular pools form the basis of a rather complex mathematical model that describes the distribution of labeled material over cellular metabolism and relates the unknown fluxes with the given measurements. This is not a real biological model but rather a physical model that provides probabilistic rules describing the distribution of isotope label. Thus, the validity of this complex model is non-critical, in contrast to mechanistic reaction kinetic models for biochemical networks in a dynamic metabolic state.
By fitting the isotope distribution model to the measured data, intracellular fluxes can finally be determined [17]. Additional information, given by the knowledge of forward and backward fluxes in bidirectional reaction steps, is generated by this method. This information can also be obtained from dynamic models that are based on reaction kinetics, if all reversible reaction steps are consequently modeled with reversible reaction kinetic formulae. This, in turn, increases the number of parameters to be estimated from the rapid-sampling data.
The standard 13C-MFA method takes rather long experimental durations, approximately 2–3 times the doubling times of the microorganism, because the label must first accumulate in the biomass before being measured. In many cases, it is not possible to keep a living system in a metabolic steady state for such a long time. Examples in which this has been achieved, however, include cells in transient growth phases, cells in industrial fermentation conditions (fed batch) or genetically unstable recombinant cells. Application to slowly growing or non-growing cells is of particular interest for plant physiology. An application that is unique to plants is given by studies on C1-metabolism in photoautotrophic plants because, in this case, the isotopic steady state contains no information on metabolic fluxes if the substrate carries only a single carbon atom [46].
Nevertheless, most physiological states of a cellular system can be considered to be in at least a quasi metabolic steady state, which means that, for short time durations (minutes to hours), metabolism is approximately in a steady state. In this case, a novel 13C-flux analysis method can be applied that does not rely on the steady-state assumption for label enrichment in metabolite pools. By contrast, the enrichment of labeled material in the metabolite pools is now observed under isotopically transient conditions ([47]; Figure 2c).
The new isotopically non-stationary method represents an interesting fusion of the methods for MFA under highly dynamic conditions that are based on rapid sampling and 13C-MFA under steady-state conditions. Metabolite pools are kept constant but must be measured because they represent the system's capacity for labeled material.
This isotopically non-stationary method (INST-MFA) became possible only recently because mass spectrometers are now able to determine reliable fractional label information from low-concentration pools of intermediate metabolites [48]. The processes for the computational evaluation of such transient data are substantially more difficult than those for the evaluation of data describing the isotopic steady state because the accumulation of labeled materials in the intracellular pools is now described by a dynamic model. Thus, the system of equations that describes the distribution of label over the network has to be replaced by differential equations [49•].
A first application of INST-MFA to Escherichia coli proved that information on all intracellular fluxes could be obtained in just 15 s, during which about 20 samples were drawn from the culture [50••]. This makes isotopically non-stationary metabolic flux analysis a promising candidate for the analysis of slowly growing plant or animal cells. Several teams are currently working to establish the new method from experimental and theoretical view-points [45•,49•,51,52].
A still-speculative extension of the INST-MFA method may be the use of experiments under both metabolic and isotopic steady states (Figure 2d). An exploratory simulation study [24] has already shown that INST-MFA would significantly improve the available information on reaction kinetic parameters in vivo and, ultimately, on the dynamic fluxes.
Another challenging development is the model-free determination of flux information from pool-size data by smoothing and differentiation of high-density data (Figure 1b). It should be noticed that this is already possible in other chemical disciplines, as has been demonstrated with the determination of diffusion flows [53].
Altogether, a detailed theoretical basis has been developed for fluxome analysis using 13C-labeling. These mathematical tools are currently adapted for fluxome studies using the FRET flux sensors. FRET sensors can provide direct information on steady-state levels and fluxes in a specific subcellular compartment. Moreover, these sensors provide information on flux in individual cells and thus significantly expand the potential of metabolomics. The combination of FRET sensors with microfluidics and modeling promises new insights into the regulation of metabolism in response to a changing environment, and into the underlying signaling networks not only in multicellular, eukaryotic systems but also in microorganisms from E. coli to yeast. Both 13C and nano-sensor MFA technologies will rapidly be applied to fluxomics, as exemplified in the case of yeast mutants that are affected in sugar signaling [54].
This work was supported by grants from Bundesministerium für Bildung und Forschung (BMBF) (SysMAP Project) to WW and from the US National Institute of Health (Roadmap Initiative 'Metabolomics technology development' [R33DK070272]) and US Department of Energy (DE-FG02-04ER15542) to WBF.
Papers of particular interest, published within the period of review, have been highlighted as:
• of special interest
•• of outstanding interest
PubMed Central Canada est un service des Instituts de recherche en santé du Canada (IRSC) offert en partenariat avec la bibliothèque scientifique nationale du Conseil national de recherches du Canada et le National Center for Biotechnology Information de la National Library of Medicine (NCBI/NLM) des États-Unis. Il renferme du contenu des archives de PubMed Central International par les éditeurs participants.


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BP Comment Quick Links
October 2, 2005
Prelude to the Playoffs
The Yankees Clinch
This was supposed to be a pitcher's duel, wasn't it? Each team had their best pitcher on the mound--Randy Johnson vs. Tim Wakefield--a pair that combined to allow just one run in 15 innings 22 days ago at Yankee Stadium. Unfortunately for those looking for a duel, the Yankees hitters pulled a Peyton Manning with the script, calling audible after audible on their way to seven runs in five innings off the majors' foremost knuckleballer.
Two at-bats had a profound impact on this game. The first took place with Wakefield on the hill and Bernie Williams standing in the batter's box in the second inning. Williams laced a first-pitch double off the glove of Kevin Millar. The ball was hit so hard that no one in Fenway, Millar included, really saw it happening. The double put runners on second and third with nobody out, and eventually both runners were driven home on sacrifice flies, making the score 5-2.
The other pivotal at-bat was a microcosm of the 2005 season for Edgar Renteria; good intentions but no results. Facing a suddenly shaky Johnson, who had loaded the bases on two walks and a single in the second, Renteria worked a 3-2 count, bringing the Fenway Faithful to their feet. Finally, Johnson decided enough was enough and blew a fastball right past Renteria for the strikeout. The inning was over, the momentum gone, and Sox fans had little reason to stand up for the duration.
This at-bat was just another example of how poor Renteria has performed in 2005. This past off-season, the argument for Renteria over Orlando Cabrera was that they were both competent defensively, but that Renteria had a decided offensive advantage. It has not played out this way:
Renteria vs. Cabrera 2005 Comparison Player BRAR FRAR WARP1 VORP Renteria 14 11 2.7 26.0 Cabrera 7 39 5.0 17.3While the decision should not be scrutinized too severely--Cabrera's FRAR numbers had declined from a peak of 57 in 2001 down to 15 in 2004, Cabrera's defensive dominance in '05 makes Renteria's offensive edge trivial, especially given Renteria's recent struggles. Looking at the Expected Runs Matrix chart, we can see that his struggles played a large role in the game. During Renteria's four at-bats, he totaled an ERM score of 2.51 runs, producing zero. This is something that is fast becoming a problem for the Red Sox offense, and is being exacerbated by the fact that Damon seems to be coming around these past two games (1-5, 3 BB, 3 SB). The more poorly Renteria performs, the less likely it is that the team can leverage the greatness of David Ortiz and Manny Ramirez.
One solution would be to insert Tony Graffanino into the two-hole and drop Renteria to the bottom of the lineup.
Sept-Oct w/Red Sox in 2005 Player AB AVG/OPS AB AVG/OPS EqA Renteria 113 .221/595 620 .276/720 .261 Graffanino 79 .316/844 187 .321/815 .280Graffanino is doing better overall, but more importantly he's better right now. Given that Graffy has hit in the two-hole this season, it should not be a shift of seismic portions.
After the Red Sox failed to score in the second, the game was largely academic. The Yankees scored again in the top of the third, further dulling any Red Sox momentum. Entering today's game, the Yankees were 61-8 when scoring six runs or more, the Red Sox 15-47 when allowing six runs or more. Johnson, who the Red Sox forced to throw 51 pitches in the first two innings, settled down nicely, needing just 71 pitches to get through the next 5 1/3 frames. After Ortiz started the third with a double, Johnson set down 16 of his last 18 hitters, surrendering only a single and a homer to Graffanino before giving way to Tom Gordon in the eighth.
There are other accolades for the Yankees in today's victory. Sheffield made two diving plays in the outfield, showing that while he may not be 100%, he can still fly around. Joe Torre also deserves credit for not screwing around in managing the bullpen. He went right to Gordon and Mariano Rivera, even with a four-run lead. Watching the White Sox take the lead on the Indians, Torre correctly realized that if the Yankees won today he could afford them two or three days' rest, and did not want to give the Sox a chance to come back. The Red Sox were able to produce a couple of runs, but never put together the string of hits necessary to start a rally, and had to watch the Yankees celebrate an eighth consecutive AL East crown on their home turf, conjuring up painful memories of 1999.
Paul Swydan is a contributor to Baseball Prospectus. He can be reached here.
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Great story by KFOR documenting the dead and dying pigeons that Senator James Inhofe left behind to slowly die after his 2016 slaughter. Click here to watch:
Read the undercover SHARK investigator's full description of the slaughter here:
Click Here
Please contact Senator James Inhofe and politely tell him to stop slaughtering animals for fun.
Tell him that it's long past time for him to end the abominable cruelty of live pigeon shoots.
Washington, DC:
205 Russell Senate Office Building
Washington, DC 20510-3603
Main: (202) 224-4721
Fax: (202) 228-0380
Click here to communicate with Senator Inhofe through Facebook!
Click here to Send the Senator a Tweet! And his Campaign Twitter Account


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This steampunk digital doll pattern bundle will let you create a Hook, O-Ring Belt and Eyepatch for your 18 inch doll.
Flamboyant adventurers, sailing the airways in their steam and wind powered ships. Good-hearted rogues, roving the world in search of daring adventure and fantastic riches. These are the Steampunk Pirates.
These accessories coordinate with other KeLibu Steampunk Pirate patterns, also available on Pixie Faire.
Recommended Fabrics: leather or vinyl
Supplies Needed:
• 1 multi-purpose plant hook or safety hook
• 8 ½ x 3 ½ inches piece of leather or vinyl
• Small piece of thin cardboard (e.g. cereal box)
• A cork that is at least 1 inch long (e.g. from a wine bottle)
• 2 x 4 inch pieces of 1 inch wide elastic (white recommended)
• 3 ½ inches of ¼ inch wide elastic
• Tailor's chalk or similar product for marking
• Hot glue or craft glue
• Hacksaw, rotary tool or strong pair of wire cutters
• Stretch or ballpoint needle
• Leather/vinyl needle
• Thread to match elastic and leather/vinyl
• Small piece of craft foam (optional)
O-Ring Belt:
• 6 ½ x 4 ¼ inch piece of light-to- mid-weight vinyl or leather
• One 1 inch brass O ring
• 6 x 3/8 inch wide rivets OR
• 21 x 3/16 inch wide brads
• Awl
• Punch (if using rivets)
• Matching thread and vinyl/leather needle (if using rivets rather than brads)
• 19 ¾ x 1 inch wide ribbon and craft glue (if using brads)
• Tailor's chalk, FriXion pen or similar
• 2 x 1 inch scrap of leather or vinyl
• 16 inches of cord OR
• 10 x ¼ inch wide vinyl/leather and 3 inches of ¼ inch elastic
• Matching thread and vinyl/leather needle
• 2 x 3/16 inch wide brads and awl (optional)
• Embellishments such as gears, skull and crossbones etc. (optional)
Skill Level: Intermediate
What You Get: One 40 page sewing pattern that you digitally download as a PDF file so you can start your project immediately! The PDF sewing pattern provides full color step-by-step illustrated instructions and full size pattern pieces. A PDF reader is required to view and print the files (example: Adobe Reader or Preview for MAC). The download link is received immediately after the transaction is complete. Print copies are NOT available.
Download, Print, Sew!
*Creating a customer account will ensure that you have the ability to access your purchased (and free) files at any time.For detailed information and answers to the most common questions, be sure to read the Terms of Use and FAQ's related to the doll clothes patterns sold at Pixie Faire.
A sporty denim alternative, this skirt has lots of options for adding details. Five pockets,...
A sporty denim alternative, this skirt has lots of options for adding details. Five pockets,...
Panniers or side hoops are women's undergarments worn in the 17th and 18th centuries to...
Create a beautiful historical fashion for your 18 inch doll! This gown is based on...
Does it get any better than an eye patch or Hook? I think not. Brilliant Job!! An absolute winner in my book!


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Posted by Denny
Steve is handling my divorce which is really complex. His knowledge of the law is outstanding and he keeps me informed. He also has a great payment plan .
Posted by Tina
Mr. Keist has helped us with with several matters and we find him to be honest, dependable and a man on integrity. He handled himself well in court and with out of court matters too.
Posted by anonymous
We paid the retainer and provided his firm all the financial information, but the bankruptcy petition he filed contained less than half of our creditors, left the statement of intention blank. After multiple phone calls and emails the office staff said they would pull the file of the amendment to make sure the corrections were added. They never sent me a complete copy of the petition, and on my own initiative, I accessed our case through PACER and discovered nothing was done to correct the deficiencies. Several phone calls were answered by staff making excuses why the person I needed to speak with was not available. They never did call me back. I filed a complaint letter with the US Trustee, and she has sent a letter to Mr. Keist asking him to respond to my allegations. We also believe Mr. Keist could have done more to help us avoid having our house sold at Trustee Auction only a week before I was due to receive a pension payout which could have reinstated our loan. An investor bought our house at the auction and resold the house a month later for $90k profit, and there went our equity.


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Buckle Inc. recently announced Emily Reid has been named manager of the Thompson Hills Shopping Center Buckle store in Sedalia.
Reid began her career with the Buckle in 2012. She was a management trainee at the Sedalia Buckle before being named manager of the Thompson Hills Shopping Center location. Reid is a graduate of the University of Central Missouri in Warrensburg with a degree in textiles and clothing in business.
Offering a unique mix of high-quality, on-trend apparel, accessories and footwear, the Buckle caters to fashion-conscious young men and women. Known as a denim destination, each store carries a wide selection of fits, styles and finishes from leading denim brands, including the company's exclusive brand, BKE. Headquartered in Kearney, Nebraska, the Buckle has more than 450 retail stores in 44 states.
Release courtesy of Buckle


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Happy Earth Day: Governor Ducey Vetoed "Land-Grab" Bills
Royce Bair/Flickr
When it comes to the environment, we don't often get a lot of good news in Arizona. But in honor of Earth Day, here's one victory to celebrate: Governor Doug Ducey vetoed two important "land-grab" bills.
Earlier this month, the Arizona Legislature gleefully passed a series of bills that demanded the federal government transfer the title to all lands it owns in Arizona to the state. (In Arizona, the federal government owns 42.2 percent of land.)
The purpose of these bills was ostensibly to raise money for state institutions and programs, which would be done by selling the land or issuing grazing leases. Critics, who dubbed these efforts "the land-grab bills," say there are other ways to fund our schools, and feel that the whole point of the bills was to appease the private sector and cattlemen. They also worry about the future management of the land, and about whether citizens would lose access to public lands.
See also: -AZ Senate Advances "Land Grab" Bills -Governor Doug Ducey Has Four Important Environmental Bills Sitting On His Desk
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"Public lands belong to all Americans. They don't belong to the state of Arizona," explains Sandy Bahr, chapter director of the Arizona Sierra Club. "The people of Arizona have been very clear about not wanting the state to take control of federal lands."
She's right, this year wasn't the first time the Legislature has attempted to take over federal land. In 2012, former Governor Jan Brewer vetoed a similar bill, and the Arizona populace rejected the measure on a ballot.
Ducey had three slightly different "land-grab" bills on his desk, and he vetoed two of them. The first, HB 2176, required the State Land Commissioner to demand the federal government give up the title to all public lands in Arizona. It also required the state Attorney General take legal action if the transfer didn't occur by January 1, 2022.
And the second, HB 2318, would have added Arizona to an interstate compact designed to help facilitate the transfer of public lands to the state. The compact is a 2014 Utah initiative that would have become effective after a second state signed on.
Ducey did sign the third bill, HB 2658, into law, but this one has less teeth than the other two. All it does is establish the "Transfer of Federal Lands Study Committee," to examine the process of transferring, managing, and disposing of federal lands within Arizona.
According to the law, the committee will:
- Conduct hearings to collect, evaluate and analyze information relating to federal lands in this state to identify significant concerns, risks, solutions and goals associated with these lands relating to the following: environmental quality; economic productivity and sustainability; public health, safety and welfare; consistency with state and local objectives; ownership and jurisdictional responsibilities; other aspects considered appropriate by the transfer of federal lands study committee.
- Consult with experts in the fields and industries associated with or related to public lands.
- Survey the county board of supervisors of counties that contain at least 15 percent land area under the management of federal agencies.
- Develop management priorities for public lands received from the federal government for this state and local jurisdictions.
- Identify measures that will ensure that public lands in this state are managed responsibly and prudently for present and future generations.
- Develop processes for this state to receive title to public lands from the federal government and to transfer title to the public lands received from the federal government.
- Investigate lawful mechanisms, including actions implemented in other states, that may aid in achieving the goals identified pursuant to paragraph 1 of this subsection.
- Submit a report regarding the committee's activities, findings, and recommendations on or before December 31, 2019 to the governor, the president of the senate and the speaker of the house of representatives and provide a copy of this report to the secretary of state.
Two out of three isn't bad. Happy Earth Day.
Got a tip? Send it to: Miriam Wasser.
Follow Valley Fever on Twitter at @ValleyFeverPHX. Follow Miriam Wasser at @MiriamWasser.
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Stylish Office Makeover
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When starting at a new office, you want to look professional and chic, which isn't too difficult for this high level businesswoman. With the most fashionable glasses, elegant pearl necklaces, and a variety of top of the line dresses, she can put together an outfit for a board meeting or just for working around the office.
Use your mouse to click on and through the floating hair, clothing, and accessory options for this stylish office girl.


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Rolls-Royce share of Thai Airways International V2500 order worth $75 MRolls-Royce, the global power systems company, has won a share of an order from Thai Airways International for V2500 engines to power five purchased and six leased Airbus A320 series aircraft. The contract includes a long-term engine maintenance agreement. The value of the contract to Rolls-Royce is over $75 million.
Deliveries will commence in 2012 and Thai Airways International will employ the new V2500s to support the creation of a new low-cost regional carrier, Thai Smile.
Related Research on ASDReports.com:
The Global Missiles and Missile Defense Systems Market 2011-2021
The Global Missiles and Missile Defense Systems Market 2011-2021
More than 4,800 V2500s in service have been delivered so far, with nearly 2,000 more engines on order and close to 200 customers around the world.
Source : Rolls-Royce Plc (LSE: RR.L)
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Michelle has made sure that I have a plentiful supply of delicious peppermint tea and chicken soup from the Cafe that she works at. It's been a lifesaver!
Today, I am up, moving around. I even pushed to vacuum. What I really need to do is shampoo this carpet and furniture. Maybe tomorrow.
The main water drainage pipe rusted and broke and now when you shower or wash dishes all of the water ends up on the basement floor. Thankfully it is in the utility area close to the floor drain. Paul is trying to fix it today.
I finally sent an email inquiry to a company that I have wanted to work for for a long time but was to afraid to do it. It is time that I stop dreaming about it and do it! Now, keep your fingers crossed that they contact me.
I have a confession to make.
Have I told you about this love affair that I am in?
Yes, as much as I try to fight it, I am madly in love.
I just can't keep my eyes and hands off!
It has been going on since December and we're inseparable.
We're always in bed together, snuggling up on the sofa, you can even find us sneaking around in parking lots and restaurants.
His name is Mr Kindle Fire.
It is 7 1/2 inches, small enough to fit inside of my purse yet large enough for me to enjoy the screen.
It had me at hello. I purchased two kindle fire's for my daughters for Christmas, and then after playing with one, I decided to keep it and give the youngest daughter a laptop instead. ( She will need the computer for high school next year anyway). Both Michelle and I love our Kindles so much that Paul wanted one too.. so we got him one.
On the kindle I can visit my favorite blogs and websites.


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
how to get a PDF of the entire document (signed)Asked by mollygarg on December 21, 2014 at 03:44 PM
Hello, I have checked the FAQ as well as the forum and can't seem to find the answer.
I use jot form to send my contract to clients to digitally sign. I receive the submission and I understand I can convert the submission to a PDF. However, what I would like to do is be able to send the client a copy of their signed contract (so the form + the signature). Is there a way to go about doing this?
Here is the form http://form.jotform.us/form/43306053699156
Thanks, Molly
Hi Molly,
If you would like a PDF version of the form filled out by your user, you can include the PDF link in your auto-responder.
The PDF will have the questions and your user response included in the PDF.
I hope this helps, please inform us if you need further assistance.


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Product Description
Lock Out Tag Out Station - Wall Sign
A wall sign that reads, "Lock Out Tag Out Station" lets everyone know where the items they need for this procedure will be located. It can also be used to put directly around the area of a machine that needs to be locked out before it is worked on. This is a very simple sign but it conveys a clear message that can help to save lives in a facility. A lock out tag out station will have all the locks, keys and other items that are needed to secure the power to a particular machine. When used properly it can virtually eliminate the possibility of someone getting hurt due to unexpected powering up of the machine while it is being worked on.
Put this wall sign up at any lock out tag out station or near the areas where the power can be locked out for a machine and you'll be reminding everyone of this important safety requirement. The sign can be hung up on a wall, post or even a machine itself.
- Mounted on a durable 3mm PVC signboard
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For full warranty information, please visit our warranties page.
Product Reviews


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WINTER OLYMPICS; Daly Is on Hand to Honor Vinnie Johnson
By AL HARVIN,
Published: February 6, 1994
AUBURN HILLS, Mich., Feb. 5— After letting the Detroit Pistons celebrate the retiring of Vinnie Johnson's No. 15 for the entire first and second quarters and a long intermission, the New Jersey Nets ended the festive mood in the Palace by going on a 33-16 surge in the third quarter on the way to a 107-100 victory tonight.
The Pistons' Joe Dumars put on a one-man show scoring 16 of his game-high 42 points in the first quarter after which the struggling 10-35 Pistons had a 30-15 lead. Lindsey Hunter came off the bench to chip in 10 of his 15 points in the second quarter when the Pistons stretched their lead to its longest of the night, 21 points.
Derrick Coleman and Kenny Anderson, however, rallied the Nets, finishing with 31 and 29 points, respectively. They each got 9 points in the third quarter and had 21 points apiece after intermission. Coleman also had a game-high 18 rebounds, one shy of his season-high 19 last November against the Los Angeles Lakers. The Longest Night
"It might be the longest night in basketball," said Nets Coach Chuck Daly, who took part in the 45-minute halftime salute to Johnson, a player on the back-to-back championship teams when Daly was coach of the Bad Boys of 1988 and 1989. Johnson delivered the winning shot against Portland in that last title game.
"I am happy I was here," Daly said. "Vinnie was some kind of player. He was one of the ultimate playoff players."
Daly, however, was not around to see the end of this game from the floor. He was tossed out with two technicals arguing a technical foul with 1 minute 47 seconds left in the third quarter and the Nets up by 73-71 going to the locker and leaving the rest in the hands of his assistant Brendan Suhr, who was also an assistant in those Pistons years.
The long halftime show featured highlight films of Johnson's career, introduction of his family, friends and teammates, including Rick Mahorn, now on the Nets injured list, the recently retired Bill Laimbeer as well as Dumars and Thomas, the only active Pistons from that team, and Dave Bing, the first Piston to have his number retired.
"This win goes to Chuck," said Suhr, who was in charge when the Pistons did not tie the score or retake the lead. "What we did in the second half is just try to keep the ball out of the hands of Dumars, and Kenny helped to do that. It was also important to have Benoit Benjamin play the way he did. He needs to have good things like this happen to him."
Benjamin, had one of his rare double doubles of the season, scoring 13 points and grabbing 12 rebounds, 9 of those rebounds and 7 of those points coming in that third-quarter comeback when the Nets erased a 14-point halftime deficit. He also had the only two Nets blocked shots in the game.
"We were just sleepwalking in the first half," said Anderson, who dished out only 4 assists to lead the Nets and match Detroit's Isiah Thomas in that category. "I think we just took their record for granted in the first half, that's all." Nets Have Won 8 of Last 10
While the Nets have won 8 of their last 10 games and are one game under .500 at 21-22, the Pistons have lost 22 of their last 24.
But the Pistons were shorthanded. Neither Robert Horry nor Matt Bullard, acquired in a trade with Houston for Sean Elliott on Friday, were able to dress since Elliott's physical examination was not yet taken to complete the deal.
"I don't understand why, but if that's the case I would like to know why," said Detroit Coach Don Chaney. "I would have liked to use the two new players tonight. We could have already used them. We just reached the point where we had to go with a real small lineup because of foul trouble."
Kevin Edwards, with 16, was the only other Net in double figures.
Photo: Pistons' Greg Anderson blocking a shot by Nets' Kenny Anderson during the first quarter last night. (Associated Press)


text:
Edging, Neoprene, G, 3/16x 5/16 In, 100 Ft L
E. JAMES| Zoro #: G0846727| Mfr #: ENP712202830
Zoro #: G0846727Mfr. Part #: ENP712202830
$128.02 /ea
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Technical Specifications
|Zoro #: G0846727 Mfr #: ENP712202830
|Backing Type: Non-Adhesive
|Item: Rubber Edging
|Color: Black
|Max. Temp. (F): 160
|Material: neoprene
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|Groove Width: 3/16"
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|Min. Temp. (F): -40
|Standards: ASTM D2000 BC
|Overall Width: 7/16"
|Overall Height: 15/32"
|Style: G
|Country of Origin (subject to change): United States
Product Description
Rubber Edging, Neoprene, G, Groove Width 3/16 In, Groove Depth 5/16 In, Overall Width 7/16 In, Overall Height 15/32 In, Length 100 Ft, Black, Non-Adhesive Backing Type, Min. Temp. Rating -40 Deg. F, Max. Temp. 160 F, Standards ASTM D2000 BC


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Expert Carpet Cleaning Tips for Families
An expert, Ben Shemesh, reveals his tips for cleaning carpets.
An expert, Ben Shemesh, reveals his tips for cleaning carpets.
Is your child highly focused? Does she ask a lot of questions? Is he a fast learner, and lazy in the classroom? These are all characteristics of a gifted child. A local expert shares more qualities to look for, and what you can do to help your gifted child.
Being an active, present father has some underlying benefits for your kids, including children having good health, performing better academically and socially, and having a good leadership role model.
Edtech is using technology in schools to help teachers teach.
Can cavities be spread from person to person? Marc Bennet, D.M.D., answers and shares what causes cavities.
Your child is due for dental X-rays at his next dental appointment. Should you be concerned about the amount of radiation? Adam Postel, D.M.D., answers.
Your child was involved in a playground accident and a tooth was knocked out. Now what? Mahnaz Khan, D.D.S., shares what to do in a dental emergency.
When a child is diagnosed with autism, he may benefit from applied behavior analysis. But what exactly is ABA and how will it help your child? We've got the answers here.
Here are 10 things to keep in mind and to look for when touring and choosing a preschool for your child.
Some women have difficulty conceiving after their first child. This is called secondary infertility. Paul Gindoff, M.D., shares the causes and treatments of secondary infertility.
Tips for parents on how to get their child to eat healthy food.
Should you use an agency or a website to find your the nanny or babysitter you will hire to care for your children? Depends what you're looking for and how you prefer to conduct your search.
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Looking for a nanny can be a daunting task. Deb Crisford, founder and director of a nanny placement service in NYC, shares five things to look for when searching for a child care provider that's a good fit for your family.
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Mina Rochel Marsow, owner of Prospect Gymnastics, shares the earliest age at which it is most beneficial to enroll your child in a sports class.
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|Derivatives of quinuclidine
|Quinuclidine derivatives having the general formula (I) ##STR1## and geometrical isomers, enantiomers, diastereoisomers, racemates and/or acid addition salts thereof, wherein Z represents the group >CR.sup.1 R.sup.2 or two hydrogen atoms; and R.sup.1 and R.sup.2, which may be identical or different, are each alkyl, cyclopentyl, cyclohexyl, aryl, or diarylmethylol, or alkyl which is substituted by one or more aryl groups, or one of R.sup.1 and R.sup.2 may be hydrogen.
|Fisher; Abraham (Holon, IL), Karton; Ishai (Ness-Ziona, IL), Heldman; Eliahu (Rehovot, IL), Levy; Aharon (Moshav Beith Hanan, IL), Grunfeld; Yona (Rehovot, IL)
|State of Israel, represented by Prime Minister's Office, Israel (Ness-Ziona, IL)
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Makhshava is a Hebrew word commonly translated as "thought." Dr. Hugh Nibley, however, maintained that "plan" is a better translation. [1] Biblical passages in which makhshava "definitely should be" translated as "plan" (emphasis in the original) include, according to Dr. Nibley:
- Jeremiah 29:11
- Jeremiah 51:29
- Psalms 33:11
- Book of Proverbs 19:21
- Proverbs 20:18
- 2 Samuel 14:14
- Micah 4:12
In addition, according to Dr. Nibley, makhshava can be rendered "plan" in:
- Isaiah 55:8,9; 59:7; 65:2; 66:18.
- Jeremiah 6:19; 11:19;18:12,18; 29:11; 49:20,30; 50:4-5.
Nibley's student Benjamin Urrutia maintains that this translation is also necessary in the Book of Esther, in which the usual translations read that Haman "thought" to destroy the Jewish people. But he did not just think about it; he made a plan. The correct translation is: "Haman planned to destroy the Jews."
In the Hebrew Bible, the concept "he thought" is expressed as amar belibo - literally "he said in his heart."
- ↑ Since Cumorah, 1988, ISBN 0875791395 , page 214
See also
Some or all of this article is forked from Wikipedia. The original article was at Makhshava. The list of authors can be seen in the page history.


text:
He only met a couple but I feel he is on a constant back up plan as he sees them daily on feed etc..
Most Helpful Guy
Remember, you can't force anyone to do anything. But if they are doing something that doesn't make you feel comfortable, you have every right to tell them that it doesn't make you feel very comfortable. My last girlfriend use to go to drinking parties without me. I didn't want to come off as an overprotective or jealous boyfriend, so I told her how I really felt but gave her the choice knowing how it felt when she did that. She went two more times and every time she came back, she asked me how I felt. I told her I still felt the same but I wasn't going to force her to do either. She stopped because she knew it meant more to me. The same should be for your boyfriend that if he cares, he'll get rid of the other girls that don't matter. You and how you feel, that's the only thing that should matter. And if he's focusing on other girls, even a little, even for a 'backup', it's still wrong. So tell him that you don't like it and let him make the right choice. I know I speak optimistically, and I'm not saying he's a bad guy if he doesn't do it, but some guy's need that womanly touch of persuasion. You know him best and you'll know what to say. Best of luck00|00|0
Most Helpful Girl
tell him it makes you uncomfortable :)00|00|0


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HTML5 code example: Intelligently store Images in localStorage for faster page loads!
bobleah Tags: code localstorage html5 22 Comments 116,882 Views
Recently I was exploring lazy load techniques as a method to improve perceived page load times. There are a number of methods to lazy load web artifacts, which enable a web developer to delay or load page elements on demand. Typically these methods are used...
HTML5 - code example of ContentEditable and LocalStorage - create a web sticky note!
bobleah Tags: html5 featured localstorage code contenteditable 23 Comments 104,161 Views
In my previous blog entries, I shared HTML5 code examples for drag & drop and Geolocation . In this blog entry I will explore two new HTML5 offerings: contenteditable and localStorage . While exploring HTML5, and reading the W3C specification, I...
HTML5 - code example of File API - drag and drop hard drive files to a webpage!
In previous blog entries, I shared HTML5 code examples for drag & drop , Geolocation , contenteditable and localStorage . Today, I will share a simple app that provides a code example of FileReader , which is part of the File API specification of HTML5. ...
HTML5 - code example of Geolocation in an iPhone
bobleah Tags: code geolocation featured html5 32 Comments 79,802 Views
In my previous blog entry, I shared an HTML5 code example of implementing the drag & drop interface. Today, I decided to explore the Geolocation API by coding up an example of its usage. The Geolocation method returns a number of values... which by...
HTML5 - code example of drag and drop on a web page
bobleah Tags: code html5 4 Comments 78,347 Views
I have recently attended a couple of analysts meetings where the subject of HTML5 was a recurring theme. With my curiosity peeked, I decided to explore what HTML5 has to offer today. Of particular interest is the drag and drop (d&d) API... as this has...
HTML5 - Example Form validation and styling Form elements
While the current web browser support matrix has not fully caught up with the promise of the HTML5 Form specification, we are seeing additional adoption of various HTML5 Form elements in some recent browser deployments. This post will cover a few basic Form...
Using Sencha Touch HTML5 library to build a mobile application
bobleah Tags: html5 qrcode javascript ibmimpact code css3 sencha css 11 Comments 64,658 Views
As in past conferences, Impact2011 offers a web based social aggregator , which pulls together conversations and artifacts from Twitter, YouTube, Flickr, Facebook, and other social destinations, into a single web page. While this page is available to mobile...
HTML5 Canvas example - an introduction to the basics: create a branded QR code Canvas widget!
The HTML5 Canvas element is a 2D drawing interface supported by most modern web browsers. Canvas enables a web developer to draw anything directly into a web browser natively, without the need for Java or Flash plugins. While the Canvas API is fairly simple...
CSS3 box-shadow property: use CSS to liven up your web images!
Shadow effects have always been very cool. What started out as Photoshop junkies attaching them to background images is now in the domain of any programmer, thanks to CSS!. The CSS3 box-shadow property allows designers and developers to easily implement...
Como ganhar dinheiro com apps?
Há alguns dias participei de uma mesa redonda em uma universidade com diversos jovens empreendedores que estavam muito motivados a criar negócios baseados em computação móvel, escrevendo apps. Aparentemente a fórmula para ganhar dinheiro com apps é simples:...
HTML5 - code example of Notifications API
bobleah Tags: code html5 notifications 45,596 Views
In my previous blog series on HTML5 code examples , I covered ContentEditable, FileReader, LocalStorage, Geolocation, Drag & Drop, and other API's, demonstrating the usage of these HTML5 interfaces. As the spec matures, HTML5 interfaces are becoming more...
Year in Review - my most popular blog posts in 2010
bobleah Tags: 2010 html5 css3 2 Comments 18,943 Views
With the year wrapping up, this is a good time to reflect back on 2010. I was kept busy in the first half of 2010 assisting with the deploy of developerWorks community applications on Lotus Connections 2.5, and expanding the developer community to fully...
Ajax file upload to a Java Servlet in HTML5
Hazem Saleh (IBM) Tags: formdata html5 servlet chrome safari firefox java ajax upload file 1 Comment 18,229 Views
HTML5 comes with a great feature which is the ability to upload files using the XMLHttpRequest version 2. Modern Gecko and WebKit browsers include a wonderful object FormData that allows combining both simple and complex form data (text and files) in the...
from Blog: Hazem Saleh
TWOdW: HTML5, JAX-RS, BigInsights, Eclipse, Lady Gaga
Scott Laningham Tags: eclipse ladygaga biginsights big_data iod2011 html5 twodw jax-rs 16,136 Views
This week on developerWorks, John Swanson and I launch the dW YouTube Channel viewer campaign, run down the week's content highlights, including the official launch of knowledge paths on dW, and talk a little more about the upcoming Information On Demand 2011...
from Blog: Scott Laningham
TWOdW: analyze assets, mitigate risks, Apache Mahout, mobile txt messaging
Scott Laningham Tags: twodw html5 mobile apache_mahout websphere rational 15,708 Views
John and I are back after breaking for the rush of IOD 2011. Today we highlight new developerWorks content on project risk mitigation, the Virtual I/O server command line interface, Apache Mahout, balancing openness and privacy, mobile text messaging in web...
from Blog: Scott Laningham


text: This painting is sure to brighten any room or dark corner. Painted with vibrant yellow, orange and blue, it evokes the beauty of vibrant sunflowers against a bright fall sky. The painting measures 16x20 inches (40.64x50.8cm). Painting is made on stretched canvas, painted blue on all sides and wire hanger attached on the back. It has been sprayed with a varnish to protect the color. All artwork is initialed and dated. Item comes wrapped in brown paper and tied with twine. .


text:
Here you can buy our CDs.
Information about how to buy a CD you find further down.
The Quattrocelli Scenes
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text: Vice Presidents are private patrons and benefactors who wish to have a closer relationship with the Festival. They receive exclusive privileges and invitations throughout the year and enjoy hospitality and complimentary tickets during the Festival. To find out more information about becoming a Vice President contact Amanda McKean, Development Manager, 01227 452853 or email firstname.lastname@example.com Canterbury Festival gratefully acknowledges the support of the following Vice Presidents: Anonymous Anonymous Brigadier and Mrs Maurice Atherton Professor Stephen Bann CBE Mrs James Bird Mr Timothy Brett Mr and Mrs Graham and Maggie Brown Mr and Mrs Christopher and Nicki Calcutt Mr and Mrs Martin and Virginia Conybeare Dr Kate Neales and Mr Peter Cox Mrs Jane Edred Wright Mrs Anna Grant Mr and Mrs Peter and Brenda Hermitage Mr and Mrs Neville and Anita Hilary Mr Andrew Ironside The Hon and Mrs Charles and Katie James Mr and Mrs George and Christine Kennedy Mr and Mrs Wolfgang and Dominique Kerck Mr and Mrs Roddy and Caroline Loder-Symonds Mr and Mrs James and Jane Loudon Mr and Mrs Ian and Juliet Odgers Mr Richard Oldfield Mr and Mrs Terry and Valerie Osborne Mr and Mrs David and Alicia Pentin Jane Plumptre Mr and Mrs John and Julia Plumptre Mr Andrea Russo Mr and Mrs Paul and Patricia Smallwood Dr David Starkey CBE Mr and Mrs Peter and Beryl Stevens Mr Richard Sturt OBE Mrs Fiona Sunley Lady Swire Mrs Camilla Swire Lady Juliet Tadgell Mrs Jo Taylor Williams Mrs Loba Van der Bijl Mr and Mrs Charles and Sally Villiers Sir Robert and Lady Worcester KBE Mr and Mrs Evelyn and Scilla Wright Platinum Supporters The Canterbury Festival is also greatly indebted to its Platinum Supporters, who help to support the Festival and provide a stable financial base for the Festival's work. To become a Platinum Supporter the Festival asks for a commitment to donate a minimum of £500 each year, for a term of four years. Benefits include: two invitations to the Festival Exhibition Private View two tickets to the Sponsors and Supporters reception and event during the Festival two further complimentary tickets across the Festival programme Advanced mailing of the brochure and priority booking dealt with personally by the Festival's Development Manager. Friends' newsletters keeping you up-to-date with the Festival and Friends' events throughout the year. Due recognition in the Canterbury Festival Brochure which has a circulation of 60,000, a listed entry on the Festival website and a listed entry in the nightly programmes for the Festival. Canterbury Festival greatly acknowledges the support of its Platinum Supporters: Professor Keith Mander Simon Backhouse Contact Amanda McKean, Development Manager, on firstname.lastname@example.com for more information.