text:
Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state
A growing body of evidence has shown that Krüppel-like transcription factors play a crucial role in maintaining embryonic stem cell (ESC) pluripotency and in governing ESC fate decisions. Krüppel-like factor 5 (Klf5) appears to play a critical role in these processes, but detailed knowledge of the molecular mechanisms of this function is still not completely addressed.
By combining genome-wide chromatin immunoprecipitation and microarray analysis, we have identified 161 putative primary targets of Klf5 in ESCs. We address three main points: (1) the relevance of the pathways governed by Klf5, demonstrating that suppression or constitutive expression of single Klf5 targets robustly affect the ESC undifferentiated phenotype; (2) the specificity of Klf5 compared to factors belonging to the same family, demonstrating that many Klf5 targets are not regulated by Klf2 and Klf4; and (3) the specificity of Klf5 function in ESCs, demonstrated by the significant differences between Klf5 targets in ESCs compared to adult cells, such as keratinocytes.
Taken together, these results, through the definition of a detailed list of Klf5 transcriptional targets in mouse ESCs, support the important and specific functional role of Klf5 in the maintenance of the undifferentiated ESC phenotype.
Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation embryos; they are amenable to manipulation, enrichment and expansion and retain the developmental potency of embryonic founder cells, being able to differentiate into cells and tissues of all three germ layers both
Klfs belong to the Sp1 family of transcription factors with over 20 members
Identification of Klf5 direct targets
To identify genes directly regulated by Klf5, we first analyzed the transcriptome changes upon Klf5 KD in ESCs by microarray analysis. To minimize indirect effects due to phenotypic changes induced by Klf5 KD, we performed a time course analysis both by qPCR and by Western blot analysis, which showed that Klf5 expression is significantly decreased already 12 hr after siRNA transfection (Figure
Figure 1
Identification of Klf5 targets by microarray analysis
Identification of Klf5 targets by microarray analysis. (a) qPCR and western blot analysis of Klf5 expression following transfection of siKlf5 and siNS at different time points (12, 24 and 48 hr after siRNA transfection). (b) Expression level of stemness markers was measured 12 and 24 hr after transfection by qPCR and Western blot analysis. All qPCR data are expressed as fold changes relative to mock-transfected cells. Error bars represent standard errors (SD) of triplicates. *
Statistically significant probes (FDR < 0.1) were classified as upregulated and downregulated following Klf5 KD by using as cut-off > 1.25- and < 0.75-fold changes, respectively. On the basis of these constraints, we identified 239 upregulated and 74 downregulated genes (Additional file
Additional file 1
Additional Table 1. Gene expression profile by microarray analysis upon Klf5 KD in ESCs. Fold change is calculated by comparing data from siKlf5 cells over the control (siNS). Cut-off >1.25- and <0.75-fold changes were used. Probes with FDR <0.1 were selected.
Click here for file
These predicted genes encode a set of proteins involved mainly in development and differentiation (Additional file
Additional file 2
Additional Figure 1. GO analysis of Klf5 targets and Klf expression profile during ESC differentiation. (a) Gene ontology (GO) annotation of the selected probe sets according to DAVID "Biological Process Classification" tool (Laboratory of Immunopathogenesis and Bioinformatics, Clinical Services Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA). (b) Expression levels of Klf2, Klf4 and Klf5 were measured by qPCR in undifferentiated (t0) and 4-day (4d) and 7-day (7d) differentiated ESCs. The data are represented as fold changes relative to undifferentiated cells.
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Additional file 3
Additional Table 2. Microarray data were compared with published results. Column siKlf5/siNS is referred to our microarray data (see also Additional Table 1). Differences with published data are highlighted in red. dw: downregulated gene, up: upregulated gene; na: data not available.
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It is reasonable to assume that Klf5-regulated genes, identified by gene expression profiling, include both direct and indirect transcriptional targets. To distinguish between direct target genes and those indirectly regulated through alterations in transcriptional networks governed by Klf5, we employed genome-wide chromatin immunoprecipitation (ChIP). We introduced a FLAG-tagged Klf5 in ESCs and generated two independent pools of stable clones. The levels of Klf5 were assessed by Western blot analysis with anti-Klf5 and anti-FLAG antibody to detect both endogenous and exogenous Klf5 (Additional file
Additional file 4
Additional Figure 2. ChIP-seq validation by ChIP-qPCR. (a) Expression level of FLAG-Klf5 stable clone pools used to prepare chromatin for ChIP-seq experiment. Western blot was stained with an anti-FLAG and anti-Klf5. (b) List of peaks validated by ChIP-qPCR. Peaks with different numbers of tags were chosen. Peak location are indicated (chr, chromosome). (c) ChIP-seq validation was performed by ChIP-qPCR using anti-FLAG antibody and IgG, as control, with extracts derived from FLAG-Klf5 and Mock transfected ESCs. The data are expressed as the amount of precipitated DNA calculated relative to the total input chromatin. Samples from 1 to 15 correspond to regions close to the following genes: Agap1, Lamc2, Fcgr3, 170009P17Rik, Tgfβ2, Smx16, Nlgn1, Epha2 (upstream region), Epha2 (downstream region), Igfbp7, Serpine1, Cyp2s1, 4930467E23Rik, AC152164, Inpp4b, respectively. Three different control regions were chosen (samples 16, 17 and 18): chr1:10573933-10573984, chr1:71481391-71481461, chr3:12034661-12034625, respectively, where no significant peaks were found. Bars represent SD of triplicates.
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Additional file 5
Additional Table 3. Results of ChIP-seq analysis. The putative binding sites of Klf5 are reported with relative number of tags for each peak and FDR. Match with gene microarray data is shown. The distance from the 5' and 3' boundaries of the Klf5-regulated genes is indicated.
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Next, we analyzed Klf5-ChIP target sequences with the motif-finding CisFinder software to search for over-represented motifs. The three best results were represented by CG-rich elements (Additional file
Additional file 6
Additional Figure 3. Klf5 binding motifs identified with CisFinder via 200-bp sequences centered at binding peaks (E-score > 22).
Click here for file
To identify primary targets of Klf5 among the genes regulated upon Klf5 KD, we matched microarray and ChIP-seq data, and we found that the relationship between target promoter occupancy and gene expression analysis is excellent. In fact, we found 161 of 313 genes whose expression is regulated by Klf5, showing a Klf5 binding site within 100 kb from the gene boundaries (Table
Table 1
List of Klf5-target genes obtained by matching microarray and ChIP-seq data. Klf5-repressed and activated genes bearing a binding site for Klf5 within 100 kb from gene boundaries are reported.
Klf5-repressed target genes
Klf5-activated target genes
Additional file 7
Additional Figure 4. qPCR validation of microarray data. Sixty Klf5 target genes were analyzed by qPCR to confirm the microarray data. Probe set of both downregulated (a) and upregulated (b) genes upon Klf5 KD is shown. Black bars represent not validated probes. The data are expressed as fold change relative to siNS transfected cells. Validated probes showed a
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To gain insights into the primary targets of Klf5, we analyzed the expression pattern of the 53 validated genes (Figures
Suppression of Klf5 target genes impairs ESC undifferentiated state
The first point we asked is that of the relevance of Klf5 direct targets in the maintenance of ESC phenotype. Among the putative Klf5 direct targets, we decided to start by analyzing 23 genes that were selected on the basis of validation by qPCR (Additional file
Additional file 8
Additional Figure 5. KD of a subset of Klf5-target genes. (a) ESCs were stably transfected with shRNA plasmids for selected Klf5-target genes or with control shRNA (shGFP) and KD was verified by qPCR. The results are represented as fold changes relative to shGFP-transfected cells. SD of triplicates is reported. (b) Percentage of undifferentiated (blue) and differentiated (red) colonies observed by AP staining upon KD of eight Klf5-target genes with a second independent shRNA. *
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Figure 2
Effects of KD of Klf5-target genes in ESCs
Effects of KD of Klf5-target genes in ESCs. (a) ESCs stably transfected with shRNA plasmids were grown for 7 days at clonal density (50-100 cells/cm2) and then stained for alkaline phosphatase (AP) activity. The results of shGFP-transfected cells (negative control) and shHck-transfected cells are shown as examples. Scale bars, 1 cm. (b) Histogram showing the percentages of undifferentiated (blue) and differentiated (red) colonies observed by AP staining after knockdown of Klf5 response genes. shKlf5-transfected cells are the positive control, and shGFP-transfected cells are the negative control. Bars represent SD of triplicates. *
Additional file 9
Additional Figure 6. Expression of early differentiation markers of endoderm (Sox17), mesoderm (Brachyury) and ectoderm (Fgf5) upon KD of eight Klf5-target genes.
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Ectopic expression of Serpine1 impairs ESC undifferentiated state
As shown above, we found many genes whose expression in ESCs seems to be negatively regulated by Klf5. Two of them, Serpine1 and Runx1, were selected for functional studies. Their Klf5-dependent regulation is confirmed by the observation that they are expressed at very low levels in undifferentiated ESCs. Furthermore, these genes that seem to be negatively regulated by Klf5 in undifferentiated ESCs showed a strong induction upon ESC differentiation when Klf5 disappeared (Figure
Figure 3
Ectopic expression of Serpine1 impairs ESC undifferentiated state
Ectopic expression of Serpine1 impairs ESC undifferentiated state. (a) ESCs were transfected with empty vector (mock) or FLAG-tagged Runx1 and Serpine1 expression vector. Western blot stained with anti-FLAG antibody show the expression level of FLAG-Serpine1 and FLAG-Runx1 24 hr after transfection. (b) ESCs transfected with empty vector, FLAG-Serpine1 or FLAG-Runx1 were grown at clonal density (50-100 cells/cm2) for 7 days and then stained for alkaline phosphatase activity (scale bars, 1 cm). The percentage of undifferentiated (blue) and differentiated (red) colonies is reported. Bars represent SD of triplicates. *
ESC-specific targets of Klf5
Next, we asked the question of the specificity of Klf5. Previous works demonstrated that Klf5 is highly expressed in skin
Additional file 10
Additional Figure 7. Klf5 KD in primary keratinocytes and Klf2 and Klf4 KD in ESCs. (a) Klf5 or NS siRNA were transfected in primary keratinocytes and Klf5 expression level was measured 12 hr after transfection by Western blot with anti-Klf5 antibody. (b) Expression levels of Klf2, Klf4 and Klf5 were measured by qPCR in ESCs 12 hours after siRNA transfection. The results are represented as fold changes. Bars represent SD of triplicates.
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Figure 4
ESC-specific targets of Klf5
ESC-specific targets of Klf5. qPCR analysis of expression changes of Klf5 target genes upon Klf5 KD in primary keratinocytes compared to results obtained in ESCs. Klf5 or NS siRNA were transfected in both primary keratinocytes, and ESCs and gene expression levels were measured 12 hr after transfection. Probe sets of both genes induced (a) and repressed (b) by Klf5 in ESCs were analyzed. Arrows indicate genes regulated only in ESCs; arrowheads indicate genes showing an opposite change upon Klf5 KD in ESCs and primary keratinocytes. All data are expressed as fold changes relative to siNS transfected cells. Error bars represent SD of triplicates.
A further aspect of the specificity of the Klf5-based regulation concerns the possibility that Klf2, Klf4 and Klf5 have redundant functions by binding and thus regulating common targets in ESCs
Figure 5
Klf5-specific targets
Klf5-specific targets. Comparison of gene expression changes following KD of Klf2, Klf4 or Klf5 in ESCs. Klf2, Klf4, Klf5 or NS siRNA were transfected in ESCs. RNA samples were collected 12 hr after transfection and subjected to qPCR. (a) Klf5-activated genes. (b) Klf5-repressed genes. Arrowheads indicate the expression changes induced by Klf5 only. All data are expressed as fold changes relative to siNS transfected cells. Error bars represent SD of triplicates.
In the past 3 years, several reports have shown Klf5 to be an important player regulating ESC self-renewal, pluripotency and embryonic development with a unique requirement of Klf5 in inner cell mass (ICM) establishment compared to the other ESC-specific Klfs
We have shown that Klf5 controls genes that play a crucial role in ESCs such as Tcl1
Klf5 has been described to play a role in various biological processes, such as the control of the stress response in the myocardium
Recently, Jiang et al.
Numerous results indicate that Klf5 plays an important role in maintaining ESC pluripotency and in governing ESC fate decisions. In this work, we have explored the mechanisms through which this transcription factor regulates ESC functions. We have identified a likely complete set of genes, putative primary targets of Klf5 in ESCs, by comparing the early changes of the gene expression profile induced by Klf5 KD and the results of ChIP-seq analysis for Klf5 binding sites. About half of these genes seem to be regulated by Klf5 in an ESC-specific manner, and Klf5-dependent gene regulation in ESCs appears to be different from that based on Klf2 and Klf4, two other members of the Krüppel-like family, previously involved in ESC functions. Finally, we demonstrated that suppression or constitutive expression of Klf5 target genes clearly impair the ESC undifferentiated state. These results contribute to the understanding of the regulatory role of Klf5 in ESCs and suggest a high hierarchical role in these cells for this transcription factor.
Materials and methods
Plasmid construction
Serpine1 and Runx1 cDNA were derived from pSport vector (NIH Mammalian Gene Collection, Open Biosystems, National Institutes of Health, Bethesda, MD, USA) by PCR
Next, these cDNAs were cloned downstream FLAG-tag in the p-CBA-FLAG vector by using
Cell culture, transfection, differentiation and alkaline phosphatase staining
E14Tg2a (BayGenomics, San Francisco, CA, USA) mouse ESCs were maintained on feeder-free, gelatin-coated plates in the following medium: GMEM (Glasgow Minimum Essential Medium) (Sigma, St. Louis, MO, USA) supplemented with 2 mM glutamine, 100 U/ml penicillin/streptomycin, 1 mM sodium pyruvate, 1× nonessential amino acids (all from Invitrogen, Carlsbad, CA, USA), 0.1 mM β-mercaptoethanol (Sigma), 10% fetal bovine serum (HyClone Laboratories, Logan, UT, USA), and 103 U/ml leukemia inhibitory factor (LIF; Millipore, Billerica, MA, USA). Neural differentiation was induced as previously described
Primary mouse keratinocytes were isolated from 2-day-old Swiss ICR(CD-1) mice (Harlan Laboratories, Correzzana, Italy) and cultured as previously described
Transfection of expression plasmids and shRNA plasmids (Open Biosystems; see Additional file
Additional file 11
Additional Table 4. Sequences of shRNAs.
Click here for file
For alkaline phosphatase staining, ESCs were cultured at clonal density (30 cells/cm2). The cells were fixed in 10% cold neutral formalin buffer (10% formalin, 110 mM Na2HPO4, 30 mM NaH2PO4 H2O) for 15 min and then rinsed in distilled water for 15 min. The staining was obtained by incubation for 45 min at room temperature with the following staining solution: 0.1 M Tris·HCl, 0.01% naphthol AS MX-PO4 (Sigma), 0.4%
RNA isolation and qPCR
RNA from ESCs and primary keratinocytes was isolated by using the Tri reagent (Sigma) and then reverse transcribed using MuMLV-RT (New England Biolabs, Ipswich, MA, USA) according to the manufacturer's instructions. Real-time RT-PCR was performed using PRISM 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA USA) and Power SYBR Green PCR Master Mix (Applied Biosystems). Gene-specific primers used for amplification are listed in Additional file
Additional file 12
Additional Table 5. Primers used for qPCR.
Click here for file
Microarrays and bioinformatics analysis
Total RNA from ESCs was isolated with Tri reagent (Sigma) and further purified with the Qiagen column kit (Qiagen, Milan, Italy). Then samples from three independent experiments were sent to Coriell Genotyping and Microarray Center (Coriell Institute for Medical Research, Camden, NJ, USA), where, after proper sample processing, cRNA were hybridized with the Affymetrix Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA, USA).
For bioinformatics analysis, raw probe intensities for each of the hybridized microarrays were normalized to gene expression levels using the dChip algorithm
Preparation of cell lysates and Western blot analysis
ESCs and primary keratinocytes were lysed in a buffer containing 1 mM EDTA, 50 mM Tris·HCl, pH 7.5, 70 mM NaCl, 1% Triton protease inhibitor cocktail (Sigma) and analyzed by Western blot analysis. The following primary antibodies were used: rabbit anti-Klf5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-Nanog (Calbiochem, San Diego, CA, USA), mouse anti-Oct3/4 (Santa Cruz Biotechnology), mouse anti-GAPDH (Santa Cruz Biotechnology) and mouse anti-FLAG (Sigma). Antibody-protein complexes were detected by HRP-conjugated antibodies and ECL (both from Amersham Pharmacia, Milan, Italy).
Chromatin immunoprecipitation
For ChIP-seq analysis, ESCs stably transfected with FLAG-Klf5 were cross-linked with 1% formaldehyde for 10 min at room temperature, and formaldehyde was then inactivated by the addition of 125 mM glycine. Then the chromatin was sonicated to an average DNA fragment length of 200 to 500 bp. Soluble chromatin extracts were immunoprecipitated using the mouse monoclonal anti-FLAG (Sigma) or mouse IgG (Santa Cruz Biotechnology) as control. Then samples from two independent experiments were sent to the DNA sequencing service of EMBL (Heidelberg, Germany) and subjected to high-throughput sequencing with Illumina Genome Analyzer platform (Illumina, San Diego, CA, USA).
For ChIP-qPCR, samples were prepared as described above. Supernatant obtained without antibody was used as an input control. qPCR analyses were performed using the ABI PRISM 7900HT sequence detection system and SYBR Green PCR Master Mix (Applied Biosystems). Primers used for ChIP-qPCR are listed in Additional file
Additional file 13
Additional Table 6. Primers used for ChIP-qPCR.
Click here for file
The amount of precipitated DNA was calculated relative to the total input chromatin and expressed as the fold enrichment relative to total input according to the following formula
ChIP-seq bioinformatics analysis
More than 13 million sequences were produced and aligned to the mouse genome (version m37) masked for DNA repeat by using Bowtie tool version 0.9.9.2 (Center for Bioinformatics and Computational Biology, Institute for Advanced Computer Studies, University of Maryland, College Park, MD, USA)
(AP): Alkaline phosphatase; (BCAM): basal cell adhesion molecule; (BMP4): bone morphogenetic protein 4; (KLF): Krüppel-like factor; (CHIP): chromatin immunoprecipitation; (DGKα): diacylglycerol kinase α; (ESC): embryonic stem cell; (GDNF): glial cell-derived neurotrophic factor; (HCK): hemopoietic cell kinase; (ICM): inner cell mass; (IGFBP3): insulin-like growth factor binding protein 3; (KD): knockdown; (KO): knockout; (LIF): leukemia inhibitory factor; (NR0B1): nuclear receptor subfamily 0, group B, member 1; (OCT3/4): octamer-binding protein 3; (OTX2): orthodenticle homeobox 2; (PITX2): paired-like homeodomain 2; (RUNX1): runt-related transcription factor 1; (SHRNA): short hairpin RNA; (SIRNA): short interfering RNA; (Sox2): SRY-box containing gene 2; (TCL1): T-cell lymphoma breakpoint 1; (TGFβ2): transforming growth factor β2.
Authors' contributions
SP was responsible for the study's conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing; LC was responsible for collection and assembly of data; CT was responsible for collection and assembly of data; FP was responsible for collection and assembly of data; SC was responsible for collection and assembly of data; LA was responsible for collection and assembly of data; DA was responsible for collection and assembly of data; VDS was responsible for data analysis and interpretation; LP was responsible for manuscript writing; TR was responsible for conception and design, data analysis and interpretation, and manuscript writing. All authors read and approved the final manuscript.
We thank Dr. Scott Frank for helpful discussion. This work was supported by grants from EC-Sirocco Consortium, Associazione italiana ricerca sul cancro (AIRC) and Italian Ministry of Health.
Establishment in culture of pluripotential cells from mouse embryos
Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
Core transcriptional regulatory circuitry in human embryonic stem cells
The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells
LIF/STAT3 controls ES cell self-renewal and pluripotency by a Myc-dependent mechanism
Oct-3/4 maintains the proliferative embryonic stem cell state via specific binding to a variant octamer sequence in the regulatory region of the UTF1 locus
Sall4 modulates embryonic stem cell pluripotency and early embryonic development by the transcriptional regulation of Pou5f1
Dissecting self-renewal in stem cells with RNA interference
A protein interaction network for pluripotency of embryonic stem cells
The biology of the mammalian Krüppel-like family of transcription factors
Sp1- and Krüppel-like transcription factors
Dynamic transcription programs during ES cell differentiation towards mesoderm in serum versus serum-free BMP4 culture
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
A core Klf circuitry regulates self-renewal of embryonic stem cells
Klf5 is involved in self-renewal of mouse embryonic stem cells
Krüppel-like factor 5 is essential for blastocyst development and the normal self-renewal of mouse ESCs
The role of Krüppel-like factors in the reprogramming of somatic cells to induced pluripotent stem cells
Developmental expression of the mouse gene coding for the Krüppel-like transcription factor KLF5
A gene encoding an intestinal-enriched member of the Krüppel-like factor family expressed in intestinal epithelial cells
BTEB2, a Krüppel-like transcription factor, regulates expression of the SMemb/Nonmuscle myosin heavy chain B (SMemb/NMHC-B) gene
Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling
BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3
Dax-1 knockdown in mouse embryonic stem cells induces loss of pluripotency and multilineage differentiation
CD9 is associated with leukemia inhibitory factor-mediated maintenance of embryonic stem cells
Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells
Microarray analysis of LIF/Stat3 transcriptional targets in embryonic stem cells
Mammalian Krüppel-like transcription factors: more than just a pretty finger
Epidermal and craniofacial defects in mice overexpressing Klf5 in the basal layer of the epidermis
Novel STAT3 target genes exert distinct roles in the inhibition of mesoderm and endoderm differentiation in cooperation with Nanog
LKLF: A transcriptional regulator of single-positive T cell quiescence and survival
Klf4 is a transcription factor required for establishing the barrier function of the skin
Transforming growth factor-β2 enhances differentiation of cardiac myocytes from embryonic stem cells
Differentiation of murine embryonic stem and induced pluripotent stem cells to renal lineage in vitro
Functional and biochemical association of Hck with the LIF/IL-6 receptor signal transducing subunit gp130 in embryonic stem cells
Mouse embryonic stem cells with aberrant transforming growth factor β signalling exhibit impaired differentiation in vitro and in vivo
Cardiac fibroblasts are essential for the adaptive response of the murine heart to pressure overload
An autoregulatory loop directs the tissue-specific expression of p63 through a long-range evolutionarily conserved enhancer
Model-based analysis of oligonucleotide arrays: expression index computation and outlier detection
Real-time quantitative RT-PCR analysis of human bone marrow stromal cells during osteogenic differentiation in vitro
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
Model-based analysis of ChIP-Seq (MACS)
Exhaustive search for over-represented DNA sequence motifs with CisFinder


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It's half past two on Thursday morning. Turkey's state of emergency just came into force and on Istanbul's central Taksim Square there is a sea of Turkish flags. President Recep Tayyip Erdogan's supporters are celebrating as if the national football team had won a soccer game.
Young men stretch four straight fingers into the air - the sign of Egyptian Islamists, which Erdogan adopted. Some jump up and down to the beat of Erdogan's election campaign song that again became a hit since the failed coup.
"We are the children of a great nation," shouts a speaker from a stage. "We are the grandchildren of Sultan Fatih," who conquered Constantinople, today's Istanbul, in 1453.
Whenever the speaker, whose voice has grown hoarse, shouts the president's name, waves of applause and cheers surge. Vendors sell red scarves with Erdogan's image.
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In Istanbul, ferries brought tired commuters across the Bosporus strait from Asia to Europe and vice versa. Buses on Taksim Square were filled with people rushing to work. Stores on the popular Istiklal Caddesi shopping mile opened their shutters. There were no checkpoints or additional security forces patrolling the streets.
It is likely that Erdogan will use the state of emergency mainly to signal his strength after the coup attempt, which temporarily made him appear vulnerable.
Already in the period before the state of emergency, he repeatedly resorted to measures that the decree now formalizes. In the days immediately after the coup attempt he had thousands of people arrested and suspended tens of thousands of government employees suspected of supporting Gulen.
Anti-government protests were not forbidden before the state of emergency, but the police ended such demonstrations with water cannons and tear gas. This included harmless events, like the recent Gay Pride parade in Istanbul.
For weeks Ankara imposed curfews on Kurdish towns in the country's southeast and media close to Gulen were forced to align themselves with the government or were shut down.
One journalist, who worked for one of these media outlets, spoke to dpa by telephone from an airport on his way to Europe.
He had to leave his family behind because his children do not have passports, he explained. "If they apply, I am not sure they will get passports," the journalist said.
"From the first hours, I condemned the coup attempt," the journalist said. "If it had been successful, I would have gone to jail. After the coup failed, we are again the target. It's an odd situation."
Before his flight, he would have liked to attend the nightly "democracy vigils" against a possible future coup. "But that would have been difficult. I would have been lynched if someone had recognised me," he explained.
The journalist believes that Erdogan used the coup attempt as an excuse to remove all of his opponents from state institutions.
"What connection should there be between coup plotters, a teacher in Erzurum, a prosecutor in Antalya and an official in the Ministry of Health?" he asked.
Erdogan has been lobbying against Gulen for the past three years, after falling out with the preacher. "Hatred against the movement has been looming for a long time," the journalist said. "Now we have entered a new stage: A stage of pogrom."


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Since 1934 Inghams has grown into one of the UK's leading specialist tour operators with enviable market positions and brand loyalty for winter Ski and summer Lakes and Mountains holidays. They also belong to one of the premier travel groups in Europe. Their successful Swiss parent company, Hotelplan, is a distinguished brand on the continent and has its own offices in several European countries. Hotelplan in turn is part of the Migros organisation, the largest grocery wholesaler and retailer in Switzerland.
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Here's the funny thing about standards: They're made to centralize people, but people often end up more decentralized after trying to adhere to them.
Adam Montville, a security and compliance manager for TripWire, observed that even though IT security standards such as CobiT 4.1, PCI DSS 2.0, ISO 27001, and NIST 800-53 are the norm, many people interpret parts of them in different ways.
This is true even at the most fundamental level of vocabulary used in the aforementioned IT security standards. Montville shares his experiences with colleagues:
Read More
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Nisolo - Ecuador Sandal in Indigo
Nisolo - Ecuador Sandal in Indigo
Nisolo - Ecuador Sandal. Color - Indigo. Leather insole with micro porous cushioning. Heel is lined with soft sheep leather for maximum comfort. Rubber sole. Note - these shoes tend to fit on the smaller size. For this reason we recommend sizing up by half a size.
Charlie & Lee
Charlie & Lee is an independently owned and operated store located on Union Street, Chinatown in beautiful Vancouver, British Columbia, Canada. We focus on independent, local, made in North America or ethically sourced goods. Everything we carry is thoughtfully chosen with quality and integrity in mind. Our goal is to fill your life and home with the things you will have and love for a long time. Shop Ilana Kohn, Black Crane, Ace & Jig, Sunja Link, Lloyd, Vere Verto, The Stowe, UZI NYC and more.
Do you have questions about this product? We're here to help!


text: Enterprise Products Partners L.P. ("Enterprise") (NYSE: EPD) today announced its financial results for the three months and year ended December 31, 2012. For the year ended 2012, Enterprise reported records for each of the following: net income of $2.4 billion; earnings per unit of $2.71 on a fully diluted basis; gross operating margin of $4.4 billion; and distributable cash flow of $4.1 billion, which included $1.2 billion of cash proceeds from sales of assets. "Enterprise had another record year in 2012," stated Michael A. Creel, president and CEO of Enterprise. "We benefited from record volumes in our fee-based businesses attributable to production growth, primarily in the Eagle Ford and Haynesville shale plays, and from strong domestic and international demand for NGLs, particularly from the U.S. petrochemical industry and exports. In 2012, our integrated pipeline system transported a record 4.3 million barrels per day of NGLs, crude oil, refined products and petrochemicals and a record 14.5 trillion Btus per day of natural gas; while our NGL fractionators averaged a record 659,000 barrels per day and our processing plants handled a record 4.4 billion cubic feet per day of natural gas on a fee basis." "We generated $4.1 billion of distributable cash flow and increased our cash distributions with respect to 2012 by 5.6 percent to $2.57 per unit. Enterprise has increased its cash distribution rate for each of the last 34 consecutive quarters, the longest period for any of the publicly traded energy partnerships, and in excess of 5 percent for each of the last eight years. In 2012, we retained approximately $1.9 billion of cash flow to reinvest in our growth projects and reduce our need to issue additional equity. Excluding $1.2 billion of proceeds from the sales of non-core assets, our distributable cash flow was $2.9 billion, providing 1.3 times coverage of the distributions paid with respect to 2012," said Creel.


text: Busy Market area- Ajmer Lights On Light Off Views: 1112 Comments A full fledged market in Ajmer, Rajasthan state of India is featured in this video. It is a busy spot where one can access a variety of products ranging from flowers to household articles. Ajmer is a pilgrim centre for Hindus as well as Muslims is situated 135 kms west from the capital city, Jaipur. Near this market is the gate of the famous Dargah Sharif; a sacred place for Muslims Tags: Ajmer, Dargah sharif, Jaipur, Hindu and muslims, Pilgrim center, Capital city, India, India tourism, Temples


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Most read
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Marshall sports student to present baseball research at 2014 National Conference for Undergraduate Research
"For better or worse, the media play a huge role in many sports," Haugh said. "I thought the media's representation of Al Oliver was interesting due to its impact on his career in baseball and its effect on his Hall of Fame status."
Dr. Jarrod Schenewark, associate chair in the college's School of Kinesiology, served as Haugh's faculty mentor and encouraged her to contact Oliver for a personal interview.
"Although I provided guidance, Betsy is a member of the Honors College and worked very independently," Schenewark said. "I would love to see her research published and find out if it makes a difference in Al Oliver's life and his chances of becoming a member of the Hall of Fame."
Haugh's manuscript, "Al Oliver: A Career Worthy of the Hall of Fame," was chosen for a poster presentation out of 4,000 submissions, according to Dr. Gary McIlvain, associate dean for the College of Health Professions and chair of the School of Kinesiology.
"Ms. Haugh was chosen because she brought something unique to the table," McIlvain said. "Like many Marshall students, she has held her own and done work worthy of national recognition. Betsy represents the caliber of students we have in the College of Health Professions and our School of Kinesiology."
Haugh will graduate in May 2014 with her bachelor's degree in sport management and marketing after only three years at Marshall. Originally from Earlysville, Va., she hopes to attend graduate school next fall to study media communications.
The 2014 National Conference for Undergraduate Research will be held April 3-5 at the University of Kentucky in Lexington. According to the conference website, its mission is to promote undergraduate research, scholarship and creative activity done in partnership with faculty or other mentors as a vital component of higher education. For more information about Haugh's research, please email firstname.lastname@example.com. For further information about this year's conference, visit http://www.cur.org/ncur_2014/ online.


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"House of Harlow"
House of Harlow black leather cuff
How rocker-chic is this bracelet? Made of black leather and gold, this cuff will transform you into a rock goddess immediately! It's over 2 inches wide and is available now at Kitson!
This little bag carries a lot of attitude. In black or red, I am loving the dramatic backdrop the red leather provides for the fierce details.Tags: accessories, leather, handbags, red, black, studded, studs, clutches, vintage styleAdded 6 Years Ago from Singer22
Staple necklace for sure!Added 6 Years Ago from Singer22
i don't know what my obsession with black and gold lately.... all I know is I'm in LOVE!
Bebe's line of holiday jewelry is definitely worth perusing. The styles are cutting edge affordable. There's even a collection created by Nicole Richie from the House of Harlow collection if you like the art deco look. This jet st... Bebe's line of holiday jewelry is definitely worth perusing. The styles are cutting edge affordable. There's even a collection created by Nicole Richie from the House of Harlow collection if you like the art deco look. This jet stone necklace is another absolute stunner!
a simply designed necklace with black geometric shapes that have an art deco feel
I love the simplicity in the design of this bracelet. From Nicole Richie's House of Harlow jewelry line.
As seen on Kim Kardashian and Nicole Richie (from her signature line House of Harlow) a great statement piece to add to your jewelry collection. Alone or layered, this necklace is nothing short of fabulous!Tags: resin, black, cosmopolitan, jewelry, fashion, 2009, necklaces, House of Harlow, Nicole RichieAdded 8 Years Ago from Pink Mascara
Amelia kept her accessories to a minimum . . . but a wide-bodied cuff would certainly have been one of her favorite few frills.Tags: leather, black, costume jewelry, cuff bracelets, womens jewelry, gold-plated, piperlime, 14 karat gold, House of HarlowAdded 8 Years Ago from Gap
Pretty leather and rhinestone ring designed by Nicole Richie for her House of Harlow 1960 line.
I really like the stuff in Nicole Richie's new line House of Harlow. This is a simple gold necklace with different size black resin triangles on it.Tags: black, fashion, accessories, style, jewelry, necklaces, House of Harlow, Nicole RichieAdded 9 Years Ago from Kitson
From Nicole Ritchie's new label, House of HarlowTags: accessories, leather, jewelry, gold, black, celebrity, rings, Nicole Richie, fashionAdded 9 Years Ago from Kitson
I can never have enough from this new line.


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Archive for September 30th, 2012
For Sale ot Trade 1988 Porsche 944 Turbo S (952) – $21,000
FOR SALE 21,000 or TRADE
1988 Porsche 944 Turbo S (952).
There were only 1874 Turbo S cars delivered to the US in 1988 and there are very few survivors and maybe none in this great of condition.
Automobiles: 'Influx of imports adversely impacting operations' – The Express Tribune
The Express Tribune
Automobiles: 'Influx of imports adversely impacting operations'
The Express Tribune
Shareholders vowed to raise their voices at the relevant forums against hostile policies to help the local automobile industry to flourish and continue being a major contributor to the national economy. Habib, speaking on the occasion, mentioned that …
GM recalls 40859 cars for possible fuel leaks – USA TODAY
GM recalls 40859 cars for possible fuel leaks
DETROIT (AP) — General Motors is recalling more than 40,000 cars sold in warm-weather states because a plastic part might crack and cause a fuel leak. The company is recalling Chevrolet Cobalt and Pontiac G5 sedans from the 2007 through 2009 model …
Brown signs two bills regulating Buy Here Pay Here used-car lots – Los Angeles Times
Brown signs two bills regulating Buy Here Pay Here used-car lots
Los Angeles Times
Assemblyman Bob Wieckowski (D-Fremont), whose AB 1534 mandates that dealers post a fair-market valuation on the cars they sell, said the measure will "help level the playing field" for consumers. Even Billy Dohring, chief lobbyist for the Independent …
Nissan well short of sales target for Leaf electric cars – Chicago Tribune
New York Daily News
Nissan well short of sales target for Leaf electric cars
Chicago Tribune
DETROIT (Reuters) – Nissan Motor Co <7201.T> is falling well short of its goal of doubling sales of its Leaf electric car this fiscal year as sales in the United States are particularly weak despite high gas prices. While Nissan Chief Executive Carlos …
Nissan offers Leaf discounts to spur salesSan Jose Mercury News
Five driverless cars that want to kill you – San Jose Mercury News
The Guardian
Five driverless cars that want to kill you
San Jose Mercury News
Driverless cars may be the motor vehicles of California's near future, thanks to legislation approved by Gov. Jerry Brown last week. They're supposed to be safer, never getting drunk or breaking traffic laws. But watch out for the ones that go bad: 1 …
Future of cars — nobody at the wheelReporterNews.com
Dream machine: California opens the road to driverless carsPittsburgh Post Gazette
Driverless Cars Raise Too Many QuestionsFox Business
all 80 news articles »
India car sales soar but where are the roads? – NDTV
India car sales soar but where are the roads?
Many millions "are crossing the wealth threshold to the stage they can afford cars", said Pete Kelly, managing director of LMC Automotive UK, adding "short-term variations in economic growth aren't likely to get in the way". LMC projected light vehicle …
Give 'Em Hill: Cars of the future — driverless? Or possessed by the devil? – San Jose Mercury News
Give 'Em Hill: Cars of the future — driverless? Or possessed by the devil?
San Jose Mercury News
Google has already been testing such cars, logging nearly 300,000 accident-free miles so far. They're touted as safer than those operated by poor fallible humans drivers. These capable cars can't get drunk or miss signal lights or be distracted by kids …
Photo Gallery: Cars in the News – USA TODAY
Photo Gallery: Cars in the News
Toyota and Daihatsu showed off the Toyota Agya, one of their jointly-developed cars, at the 20th Indonesia International Motor Show in Jakarta. By Adek Berry, AFP/Getty Images. Related storyFullscreen. None. Visitors at the Indonesia International …
'Cars Land' opens in Disneyland with wheels, grins and fun – Deseret News
Deseret News
'Cars Land' opens in Disneyland with wheels, grins and fun
Deseret News
Visitors to California Adventure's new Cars Land can race through Ornament Valley in high-speed slot cars designed to look like the cars in "Cars" and "Cars 2." Disney/Pixar. Summary. The new "Cars Land" park that opened June 15 in the California …


text: He studied engineering in Germany after his schooling in the Doon School, and in 1966 joined Eicher Tractors, started by his father. He retired from the Chairmanship of the Eicher Group in 1997, and has since then been involved in a number of voluntary organizations. He is the President of Common Cause, a Public Interest Organization. He is active in running the Shroff Charity Eye Hospital in Delhi and the Good earth Education Foundation, which runs over 70 Balwadis and schools in Alwar district. He is also Trustee of Vikram Sarabhai Foundation. As a hobby he publishes high quality maps of Indian cities and tourist guides for various places of interest in India


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Cosmo Girl is an Atomic County character. He was developed by Seth Cohen.
Marissa in the Atomic County universe is CosmoLass (Cosmo Girl initially) and she's still alive. In this universe she dates Kid Chino (Ryan Atwood). She possesses a magic bottle (that gives her superpowers) and she has a car named Cosmobile. In the animated series she has a lot of relationships, Battle Axe, Johnny tears, All American Boy and The Mentalist. Her archenemy is Mistress Widow, in the 7 episode she discovers that her mother is Mistress Widow and that one of her allies and ex-boyfriend (All American Boy) has an affair with her.
- Agility
- Attractive Female
- Blast Power
- Power Item
- Unarmed Combat
based on Marissa Cooper played by Misha Barton.


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Pins are a great way to help spread the word about Recovery Ministries in your local diocese or parish. Even though our pin is "fun", the message is life and death serious, and the designated drivers we know who have proudly worn our emblem are happy to tell everyone about the gift of sobriety as well as how to get more information from the Recovery Ministries website.
$2.00 - Packet of 10 Pins


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Eli Mirzoeff
Lifelong Precious Gem Trader and Entrepreneur
About Eli Mirzoeff
As founder and head of East Continental Gems in New York City, Eli Mirzoeff brings a lifetime of experience in the precious gem and jewelry trade to his position, along with an outstanding record of client satisfaction. At East Continental Gems for more than four decades, Eli Mirzoeff oversees a small, service-oriented jeweler and precious stone purveyor notable for its relationships to major industry retailers across the globe. Producing one-of-a-kind jewelry that features the highest-quality diamonds, rubies, emeralds, sapphires, and other gems, East Continental Gems' creations can be found at top department stores and boutiques in New York, London, Paris, and elsewhere.
Aside from his responsibilities at East Continental Gems, Eli Mirzoeff enjoys spending time with his family and plays an active role in philanthropy, donating to educational, religious, and other organizations that help individuals and families in need. A third-generation gem and jewelry trader, Mr. Mirzoeff began his career while a teenager growing up in Mumbai, India, where he learned under the tutelage of his father.


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Leonora Angeles is an associate professor in the School of Community and Regional Planning at the University of British Columbia. She is concerned with gender and international development planning; women, gender, and globalization; good governance and gender planning in development bureaucracies; politics of womens movement in SEA
She has a B.A., M.A. (Philippines) Ph.D. (Queen's), Assistant Professor Leonora Angeles is a joint faculty member at the School of Community and Regional Planning and the Women's Studies Programme. She is also faculty research associate at the Centre for Human Settlements, the Centre for Research on Women's Studies and Gender Relations, and the Centre for Research on Southeast Asian Studies. Her continuing research interests are feminist perspectives on international development, gender and globalization, agrarian issues, human development and security linkages, states and elites in Southeast Asia. Her current research projects are globalization and feminization of export manufacturing (garments and semi-conductors industries) in SEA; gender, poverty reduction and bureaucracy in the Philippines and Vietnam; and gender analysis social capital and good governance in participatory development projects. She is currently involved in curriculum development and the integration of gender analysis and participatory methods for the five-year capacity-building project on Localised Poverty Reduction in Vietnam based at the Centre for Human Settlements.
Her co-edited book "Learning Civil Societies" deals with how localized communities embrace the progressive qualities of civil society is a critical topic in an era where diverse and divergent forces often counteract civil society formation and community initiatives. This collection explores the theoretical underpinnings of democratic planning and governance in relation to civil society formation and social learning.
The contributors to this volume use multiple lenses to uncover the challenges of democratizing planning and governance, helping to create a better understanding of how civil societies learn from their experiences, and how these lessons might be applied in other contexts. Learning Civil Societies provides insights for developing a critical methodology for studying civil societies and their formations and suggests that new organizational mechanisms within and outside civil societies must be created if more democratic forms of planning and governance are to emerge, be revitalized, and become institutionalized in the coming decades
Learning Civil Societies: Shifting Contexts for Democratic Planning and Governance, Green College Thematic Lecture Series, Edited by Penny Gurstein and Leonora Angeles (University of Toronto Press, 2007)


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Gabriel Stone and the Wrath of the Solarians(2014)
(The second book in the Gabriel Stone series)
A novel by Shannon Duffy
Gabriel Stone is back from Valta, but the adventure is just beginning! His friend Tahlita is trapped in Willow Creek with no memory of Valta. Gabe, Piper, and Brent are determined to reunite Tahlita with her father. Yet even as they do, Prince Oliver arrives asking for their help. Menacing forces known as the Solarians have kidnapped the Empress and Princess and are threatening the lives of humans everywhere. Along with talking tracker monkey, Finley, the friends battle vampire mermaids and a living, blood-filled lake in their quest to save the Empress and Princess. Even with cool new powers, Gabe discovers their strongest weapon is their friendship as they battle the Solarians and rescue a cursed white witch - who may be the only one who can save Valta.
Used availability for Shannon Duffy's Gabriel Stone and the Wrath of the Solarians
See all available used copies of this book at Abebooks UK or Abebooks US
Paperback Editions
April 2014 : USA Paperback
|Title: Gabriel Stone and the Wrath of the Solarians
Author(s): Shannon Duffy
ISBN: 1-939765-05-6 / 978-1-939765-05-5 (USA edition)
Publisher: Month9books
Availability: Amazon UK Amazon CA
Audio Editions
April 2014 : USA Audio edition
|Title: Gabriel Stone and the Wrath of the Solarians
Author(s): Shannon Duffy
Publisher: Audible Studios
Availability: Amazon
Genre Pages


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Moving country, Bo's first day at school, finally moved into Pottery Cottage 16 months after buying it, oak floor fitting, full house for the next week, car troubles - clearly loads of spare time, so why not sign up to sit (or stand in my case) for a portrait for a week?!
It is pretty tedious to stand still for a fairly long period of time, especially as most of my supposed thinking time goes into concentrating on keeping the pose and not falling asleep. Really happy to be doing it though, mostly to see how classically trained portrait artist Lucy Corbett works and cool to how the portraits - by Lucy and her student - are turning out.
More on this, and from the last weeks in Colonia, Buenos Aires and first days in Wales soon.......after Easter most likely, and I promise to stop being lazy and post words as well as pictures.


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About the Book
"The Tree with Hoarfrost" is a picture book wherein a fable-like story is blended with photographs of intimate snowy landscapes. The story is told in the manner of both poetic existentialism and magical realism, written for young adults.
This is a nonlinear narrative, set in the World War II and in contemporary time, and it has several layers and depth for a reader to experience. The beginning starts with a book of Hans Christian Andersen fairy tales that belonged to the author. It leads a reader into the lives of mythological protagonists in a language of symbolism and the photographs of snowy landscapes.
While the protagonists have their own life, they also stand for allegories for feelings, sensuality, human condition, and transformative power of love, recognised and sensed in author's personal life.
Features & Details
- Category Arts & Photography
Standard Portrait, 7.75×9.75 in, 20×25 cm
38 Pages
- Publish Date May 09, 2014
- Language English
- Tags Fable, Magic Realism, Short Story, Fantasy, Existentialism, Intimacy, Sensuality, Photography, Poetry
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Analytics Lead
Foster City, California
January 31, 2017
Position Type:
Full Time
Product Management
Do you want to use data-driven analytics to answer strategic questions for one of the world's most respected and innovative payments companies?
Visa has built one of the world's most advanced payments networks. It's capable of handling more than 20,000 transactions per second, with reliability, convenience and security. With over 70 billion transactions processed by Visa every year, we have a rich source of data to help our payment industry partners plan for the future.
We offer a range of branded payment product platforms, which our financial institution clients use to develop and offer credit, charge, deferred debit, prepaid and cash access programs to cardholders. Visa's card platforms provide consumers, businesses, merchants and government entities with a secure, convenient and reliable way to pay and be paid in 170 countries and territories.
The Account Level Management platform is a processing platform that provides services for segmenting client portfolios, processing issuer submitted data, spend assessment calculations, and card fee billing to support many of Visa's premium card products. This group works with different internal stakeholders including Product Office, Account Execs, Client Support, Pricing, Analytics, Technology and many others.
The Lead, ALM Analytics will be a critical member of the team and will have responsibilities for leading the blueprinting, maintaining and enhancing the ALM Analytics Environment and performing analysis on client file processing and spend assessment results to reduce risk, ensure system accuracy and improve transparency and uncover potential problems upfront. The Lead will leverage knowledge of big data, database and cutting edge data mining techniques as well as modeling and segmentation. Based on findings from technical data analysis, s/he will draw insights and make actionable recommendations that reflect the client's business and context.
The position will be based at Visa's headquarters in Foster City, California.
System accuracy KPIs design and development and deployment
Development of a strategic analytics vision aligned to business goals in the respective markets and executing with precision
Translate business problems into an appropriate analytical framework and generating insights
Execute on the analytic plan with appropriate data mining and analytic techniques
Manage multiple analytics requests and priorities at the same time
Work with senior leaders and business partners to understand critical business problems and areas of opportunity, influence to implement recommendations
Manage and execute projects independently from end-to-end; work with cross-functional and cross-cultural teams to deliver on these projects
Foster analytic competency to support and increasingly guide fact-based and timely decision-making
Be an SME and thought leader on analytics and ALM, enhancing ALM analytic capabilities, with a strong understanding of visa process
Be an advocate for analytics inside and outside Visa
Identify and respond to analytics / data trends and opportunities
Champion the use of data-driven decision-making
Potential Team management
Exposure or experiences to BI tools to capture opportunities to create and automate repeatable analyses or build self-service tools
We are looking for a strong analytic thinker and problem solver, with a blend of business and technical skills:
Business skills and general qualities:
Multi-disciplinary thinking skills: creative, analytic, strategic
Flexible and creative thinker with the ability to define business tradeoffs, generate out-of-the-box solutions and manage uncertainty
Strong project and people management skills
Detail oriented - is expected to ensure highest level of quality/ rigor in analysis
Ability to execute and solve problems independently when necessary
Good process/platform management and nice to have payment industry knowledge
Able to communicate data and technical concepts to a business audience
High level of competence in Excel and PowerPoint
Team-oriented, energetic, and collaborative style
Intellectually curious and continuously striving to learn
Experience managing and working with geo-distributed team is a plus
Global mindset, desire and demonstrated ability to work cross-culturally and in a matrixed organization
Primary Technical Skills:
Strong data science background and well-versed in different machine learning methodologies to be able to explore and recommend best analytical solutions to critical business needs
Superior analytical and problem solving skills, with demonstrated intellectual and analytical rigor
Experience with creating complicated queries in common database (DB2, HBase, others)
Real world experience using Hadoop and the related query engines (Hive / Impala).
Proficiency with SAS as a statistical package
Unix proficiency is must
Secondary Technical Skills:
Exposures to reporting tools like Tableau, Microstrategy, excel VBA, and any other BI tools is a plus
Experience programming in Python and Spark is a plus
Experiences with open-source analytics packages is a plus
10+ years of business experience, with a minimum of 5 years involved with analytics, preferably with banking / payments industry
Prefer to have a deep understanding of card / payment business
Strategic and analytical skills, with the ability to synthesize and organize data/information into actionable recommendations with clear customer and shareholder impact
Must be team oriented, collaborative, diplomatic, and flexible, with excellent presentation skills, including strong oral and written communication
Experience managing teams
Minimum of 4 years of experience of actually hands-on executing analysis involving large volume of data using common analytical/statistical software in relevant settings such as analytics, business consulting, or other data-driven functions
Experiences with monitoring production system to detect and investigate anomalies under tight time frame
Preferred to have Post Graduate degree in quantitative field such as Statistics, Mathematics, Operational Research, Computer Science, Economics, Engineering or equivalent experience
About Visa:
Common Purpose, Uncommon Opportunity. Everyone at Visa works with one goal in mind - making sure that Visa is the best way to pay and be paid, for everyone everywhere. This is our global vision and the common purpose that unites the entire Visa team. As a global payments technology company, tech is at the heart of what we do. CyberSource, a Visa company, has been and continues to be a pioneer within the e-Commerce Payment Management world. Our VisaNet network is capable of handling over 65,000 transaction messages per second for people and businesses around the world, enabling them to use digital currency instead of cash and checks.
We are also global advocates for financial inclusion, working with partners around the world to help those who lack access to financial services join the global economy. Visa's sponsorships, including the Olympics and FIFA™ World Cup, celebrate teamwork, diversity, and excellence throughout the world. If you have a passion to make a difference in the lives of people around the world, Visa offers an uncommon opportunity to build a strong, thriving career. Visa is fueled by our team of talented employees who continuously raise the bar on delivering the convenience and security of digital currency to people all over the world. Join our team and find out how Visa is everywhere you want to be.
"Visa will consider for employment qualified applicants with criminal histories in a manner consistent with the requirements of Article 49 of the San Francisco Police Code."
A little about us:
As a global company at the forefront of the electronic payments industry, Visa is a dynamic organization.
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Children's Play Mats | Kids Playroom Flooring | Custom Play Mats
All kids need a soft place to play. Whether you have a baby learning to crawl, a toddler just beginning to walk or an older child who loves to play and roll around, SoftTiles Interlocking Foam Mats are the perfect flooring to create soft, cushioned, designer playrooms. SoftTiles are designed with the safety of your kids in mind. Our children's play mats are thicker and more cushioned and have specially made sloped border pieces to help prevent tripping.
• Soft- Helps prevent bumps for newly walking or crawling kids.
• Versatile- Use directly on hardwood floors, concrete, or low pile carpet.
• Waterproof- Easy to Clean. Liquids aren't absorbed and can be wiped right off.
• Non-toxic- BPA, Phthalate, Latex free, Formamide free. No fire retardants.
• Highest Quality- Durable 5/8" thick mats made in Taiwan
• Easy to Install- Do it yourself- create large rooms or small area rugs.
Create your playroom floor exactly the way you want it!
Choose the color of the kid's foam mats, some of our fun shapes- Safari Animals, Nautical Theme, Dinosaurs, Sea Animals, etc, and add a name. You can design your floor yourself in our Mat Builder or contact us and we can help you design your floor. Custom kids flooring mats take 2-3 weeks to produce.
Please call us at 888-477-6532 or 513-860-2711 or email us for more information.
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Playroom Flooring Design Ideas
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Guess Who's Coming to Dinner (1967)
Plot Summary
After a period of vacation in Hawaii, Joanna "Joey" Drayton returns to her parents' home in San Francisco bringing her fiancé, the high-qualified Dr. John Prentice, to introduce him to her mother Christina Drayton that owns an art gallery and her father Matt Drayton that is the publisher editor of the newspaper The Guardian. Joey was raised with a liberal education and intends to get married with Dr. John Prentice that is a black widower and needs to fly on that night to Geneva to work with the World Health Organization. Joey invites John's parents Mr. Prentice and Mrs. Prentice to have dinner with her family and the couple flies from Los Angeles to San Francisco without knowing that Joey is white. Christina invites also the liberal Monsignor Ryan, who is friend of her family. Along the day and night, the families discuss the problems of their son and daughter.- Written by Claudio Carvalho, Rio de Janeiro, Brazil
When Joanna Drayton, a free-thinking white woman, and black doctor John Prentice become engaged, they travel to San Francisco to meet her parents. Matt Drayton and his wife Christina are wealthy liberals who must confront the latent racism the coming marriage arouses. Also attending the Draytons' dinner are Prentice's parents, who vehemently disapprove of the relationship.- Written by Jwelch5742
Joey Drayton brings her fiancé, Dr. John Prentice, home to sunny San Francisco to meet her affluent parents. Their liberal persuasions are now put to the test, for although the young man is an ideal choice (he's highly and internationally respected in the medical field, and he's impeccably mannered, handsome, well dressed and of a respectable California family), he's black. The film, which covers one busy day in the Drayton home, is essentially a drawing-room comedy, a series of cross-conversations between the young doctor and the girl's parents, and finally between all sets of parents and offspring. A simple dinner is extended to include the doctor's parents, who fly up from Los Angeles for the evening, and the crusty but benevolent old Irish priest, a friend of the family. Thus, the title of the film . . .- Written by filmfactsman
A couple's attitudes are challenged when their daughter introduces them to her African American fiancé.
The movie concerns Joanna Drayton, a young white American woman (Houghton) and a man with whom she's had a whirlwind romance...See full synopsis » (Warning: contains spoilers!)


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Seven Americans among the abducted in Algeria as more details emerge
An undated handout picture released by BP on Wednesday shows their operation at the In Amenas field in the Sahara desert, 1,300 kilometres (810 miles) southeast of Algiers, close to the Libyan border (Photo: BP PETROLEUM COMPANY / AFP)
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Islamist militants attacked a gas field in Algeria on Wednesday, claiming to have kidnapped up to 41 Westerners including seven Americans in a dawn raid in retaliation for France's military intervention in Mali, according to regional media reports.
A Mauritanian news agency, ANI, said the raiders were commanded by Mokhtar Belmokhtar, a veteran Saharan jihadist and smuggling kingpin. Here are some facts about Belmokhtar.
Linked to a string of kidnappings of foreigners in North Africa in the last decade, Algerian-born Belmokhtar has earned a reputation as one of the most daring and elusive Islamic jihadist leaders operating in one of the remotest corners of the globe - the vast Sahara desert.
"He's one of the best known warlords of the Sahara," said Stephen Ellis, an expert on organized crime and professor at the African Studies Centre in Leiden, the Netherlands. He said Belmokhtar had also gained notoriety as a Saharan smuggler, especially of cigarettes.
French intelligence dubbed Belmokhtar "the uncatchable" in 2002.
The White House said Wednesday it was closely monitoring the situation in Algeria after armed Islamists seized 41 hostages including seven Americans in an attack on a gas field.
"We are monitoring the situation closely and are in touch with the Algerians and our other partners in the region," National Security Council spokesman Tommy Vietor told AFP.
Algeria's APS news agency said two people were killed, including a Briton, and six injured in the dawn attack by suspected Al-Qaeda loyalists in Tigantourine in southern Algeria.
An Algerian who among the hostages was released, according to APS.
The Qaeda-linked group demanded an end to French military operations against Islamists in northern Mali in return for the safety of dozens of hostages, Mauritania's ANI news agency reported.
In a statement BP said the site was "attacked and occupied by a group of unidentified armed people," and some of its personnel are believed to be "held by the occupiers."
The number and identities of the hostages was still unclear, but Ireland announced that a 36-year-old married Irish man was among them, while Japan and Britain said their citizens were involved as well. A Norwegian woman said her husband called her saying he had been taken hostage.
In addition to those killed - one of them a Briton - six were wounded in the attack, including two foreigners, two police officers and two security agents, Algeria's state news agency reported.
Algerian forces surrounded the kidnappers and were negotiating for the release of the hostages, an Algerian security official based in the region said, adding that the militants had come from Mali. He spoke on condition of anonymity because he was not authorized to speak to the press.
But according to an AFP report, Algeria said it would not negotiate with "terrorists."
French President Francois Hollande launched the surprise operation in its former West African colony on Friday, with hopes of stopping al-Qaida-linked and other Islamist extremists he believes pose a danger to the world.
Wednesday's attack began with the ambush of a bus carrying employees from the gas plant to the nearby airport but the attackers were driven off, according to the Algerian government, which said three vehicles of heavily armed men were involved.
Attacks on oil-rich Algeria's hydrocarbon facilities are very rare, despite decades of fighting an Islamist insurgency, mostly in the north of the country.
In the last several years, however, al-Qaida's influence in the poorly patrolled desert wastes of southern Algeria and northern Mali and Niger has grown and it operates smuggling and kidnapping networks throughout the area. Militant groups that seized control of northern Mali already hold seven French hostages as well as four Algerian diplomats.
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So I have noticed on several occasions men saying that makeup is oh so horrible or they will give horrible makeup tips on what they *think* they want. These same men will look at girls in pornos, mens magazines like maxin, playboy, etc., models on tv, etc. who are completely done up. Then they turn around and tell girls that they must be ugly if they wear more than a bit eye liner or they say its a turn off. Um bitch was it a turn off when you were jacking off to a girl with ten pounds of makeup on a her face a few minutes ago? lol
I think the best thing I've heard *from a guy* is that men don't dislike makeup they don't want their girlfriend wearing it for other people, as it usually (if done well) attracts more male attention and that just increases competitions. so, he said that they don't mind girls getting dolled up for their man but if its out in public with lots of men they don't like it.
a girl told me (and I definitely agree with this) that men don't dislike makeup and the look itself generally (though a few genuinely do) but they either 1) like the natural looking makeup (which definitely doesn't mean "less makeup" as achieving a natural flawless look can take time ) or 2) they simply don't like the idea of makeup. it scares them because somehow, in the amazing logic that is the average male mind, it equates to putting on a mask and if she hides her looks then its a sign that she might be hiding something else and who wants that kind of gf.
What do you ladies (and guys too) think about all of this?
i love makeup btw. I'm very good with it, I watch beauty blogs on youtube, help my friends with it, etc. don't wear it 24/7 but I still love it. :)
Most Helpful Guy
I just had a chat about this...
Here are a few BS sayings I've heard guys say:
1) I like a girl who doesn't ware make-up.
What we mean is we like a girl to wear make-up as long as she doesn't look like a clown. Why would be object to you doing things that make our boner harder and our pride / ego bigger when in public so we can show you off? Mask your imperfections, I am A-ok with that.
2) I don't like a girl with fake boobs
Okay this is a confusing one, Fake C vs natural C, we'll take the real ones. But A cups vs fake C, we'll take the C cups. I've never met a guy who would turn down fake breast (unless they're the extreme junk).
3) I don't look at porn.
Free porn and we're not looking at it to beat off?
give me a break.00|00|1
You don't look at porn? That is great, because I thought after looking at this site that all guys did that. I myself don't watch it either and don't plan on EVER watching it.
I think you misread, all guys do that (85%).
Shery you were born in the wrong generation if you want a guy who doesn't look at porn. that is a rare breed of male these days lol


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Families Seeking Insurance For Kids
Downturn Forcing Parents to Get Aid
Wednesday, January 14, 2009; Page B01
Rising unemployment and the sinking economy are driving sharp increases in the number of Washington area families seeking state health insurance for their children, and more of these families are qualifying for coverage, records show.
The increases are particularly pronounced in the region's largest and wealthiest jurisdictions as employers cut benefits and eliminate jobs. In Fairfax County, for instance, requests for the state's insurance program for children, Family Access to Medical Insurance Security (FAMIS), were up 16 percent between November 2007 and November 2008. In Alexandria, caseloads increased 20 percent, and in Loudoun, they were up 16.5 percent.
Overall, caseloads in Northern Virginia shot up 18 percent during that period, from 19,299 to 22,692, compared with 7 percent for the rest of the state.
In Maryland's Washington suburbs, caseloads for the state's insurance plan for children have increased about 4 percent, from about 45,000 to 47,000.
Not only are caseloads up, but the number of people applying for such coverage has increased substantially too. Last week, the Virginia Department of Medical Assistance Services reported that the number of people applying for
FAMIS increased an average of 24 percent a month in 2008 over 2007, from 5,073 a month to 6,291. This included a 40 percent increase in applications in September and October 2008 over the same two months in 2007. In addition, the number of applications approved increased 10 percent statewide from 2007 to 2008.
In Maryland, where eligibility levels are more generous than in Virginia, officials in Montgomery, Prince George's and Howard counties said they have seen an increase in applications for the Maryland Children's Health Program (MCHP) as well. For instance, the number of people applying for the program in Montgomery County increased almost 16 percent in October 2008 from a year earlier, from 3,977 to 4,599.
The District does not keep separate statistics on the number of children enrolled in its program, officials said.
"These generally seem to be people who, when times were good, didn't need this kind of help, but now with the economic downturn and loss of a job, now they may need it," said Sandy Ovuka, public assistance program manager for the Fairfax County Department of Family Services.
Social services officials and advocates for the poor say the numbers reflect the worsening recession and the larger problem of millions of uninsured Americans. There is a direct correlation between the increase in the number of children seeking state-sponsored coverage and the number of adults who lose their jobs or can no longer afford employer-sponsored coverage, national studies have found.
Although more families are finding that they qualify for coverage for their children, many of the parents still make too much money to qualify for government-sponsored coverage, such as Medicaid, for themselves. The increase in requests for assistance also burdens already stretched state budgets.
Each state runs a children's health insurance program paid for by a combination of local and federal dollars that covers families whose annual income is too high to qualify for Medicaid. Generally known as the State Children's Health Insurance Program (SCHIP), the eligibility requirements are usually much more generous than publicly financed health insurance for adults who are not elderly or disabled. In Virginia, a family of four making less than $42,400 a year -- or 200 percent of the federal poverty level -- is eligible for the insurance, although some states add money to expand the coverage to a broader group. In Maryland, a family of four making up to $63,600 qualifies for some health care aid for their children.


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USA: Trade commission agrees to anti-dumping duties on Chinese and Russian magnesium
A US trade panel has given preliminary approval to anti-dumping duties on magnesium from Russia and China used to make lighter and more fuel-efficient vehicles.
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Monday Oct 13th, 2014
How Pre-Schoolers Can Predict Disease Outbreaks
By Alexandra Sifferlin @acsifferlin [TIME]
Pre-schoolers might be the key to identifying the next big disease outbreak, finds a new study soon to be presented at the American Academy of Pediatrics national conference.
The idea is simple—the researchers created an online disease surveillance system that allows child care staff to log symptoms, like fever or stomach flu, that they see in the young kids they care for. Nearby public health departments have access to the real-time data, which helps them quickly spot emerging trends. Health officials can then loop back to the child care staffers about a spreading illness, along with instructions on how to handle it, so that the caretakers can prepare for it and alert parents. [full article below]
'The Walking Dead' Season 5 premiere seen by 17.3 million viewers
By Karen Butler
NEW YORK, Oct. 13 (UPI) -- Sunday's Season 5 premiere of The Walking Dead delivered the highest ratings in series history, AMC declared Monday.
The zombie-apocalypse show's return was watched by 17.3 million viewers, the cable network said.
"It's a Dead man's party. Who could ask for more?" Charlie Collier, the cable network's president, said in a statement. "Enormous thanks to our friends and executive producers Robert Kirkman, Scott Gimple, Greg Nicotero -- who also directed last night's terrific premiere -- Gale Anne Hurd, Dave Alpert and Tom Luse and all of their fellow producers, cast and crew for everything they do to make the No. 1 show on television such a unique and incredible experience for the fans."
The previous ratings record for an episode of The Walking Dead was 16.1 million total viewers for the Season 4 premiere.
Sunday night's Season 4 debut of Talking Dead, the live aftershow of The Walking Dead, was seen by 6.9 million viewers. The program is hosted by comedian Chris Hardwick.
'Broken man Pistorius has lost it all' - defence
The Scotsman
OSCAR Pistorius is a "broken man" after killing girlfriend Reeva Steenkamp because he lost the woman he said he loved as well as his reputation, friends, income and sense of self-worth, a psychologist called by the defence testified yesterday.
Dr Lore Hartzenberg gave the testimony ahead of the runner's sentencing for culpable homicide, and it was almost immediately characterised by the chief prosecutor as unbalanced.
Dr Hartzenberg said the double-amputee runner had sometimes cried, retched, perspired and paced up and down during meetings in which she tried to assist him.
The testimony was part of an effort by the runner's legal team to persuade Judge Thokozile Masipa that Pistorius has suffered emotionally and materially for what he said was an accident and that he is remorseful. The team hopes the judge will be lenient when she sentences Pistorius after what is expected to be about a week of legal argument and testimony. [full article below]
'Goldilocks' burglar found asleep in elderly couple's bed
By Victoria Ward [The Telegraph]
An elderly couple returned from a National Trust holiday to find a "Goldilocks" burglar asleep in their bed, a whiff of perfume in the air, the bath full of bubbles and the intruder's underwear hanging out to dry.
Pat Dyson, 73, and her partner Martin Holtby, 78, were alarmed when they got home to find a chicken fillet and pasta dinner had been prepared in the kitchen and their dinner plates and newspapers neatly stacked.
As they opened the door, they noticed their post had been tidied away and the cordless telephone moved from its base on a table in the hallway. The couple went into the kitchen and found pasta, processed cheese and chicken fillets on the worktop.
Mr Holtby went upstairs and found Polish immigrant Lukasz Chojnowski, 28, snoozing under the bed clothes.
Details of the bizarre break in emerged at Burnley Crown Court, where Chojnowski, an upholsterer, pleaded guilty to burglary and theft. [full article below].


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Hello My Name Is...
Jacob Davenport
This is my first year at Stonewall Jackson Middle School and I am excited to be working with such talented young voices in 6th, 7th, and 8th grade! We will be spending our time learning about vocal technique and vocal health, music literacy and music theory, and of course we will be singing!
Please feel free to reach me at email@example.com
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Entrepreneurship in Services and Socially Disadvantaged in India
No abstract is available for this item.
If you experience problems downloading a file, check if you have the proper application to view it first. In case of further problems read the IDEAS help page. Note that these files are not on the IDEAS site. Please be patient as the files may be large.
|38 pages
|Date of creation:
|Oct 2011
|Date of revision:
|Oct 2011
|Contact details of provider:
| Postal: 420 West 118th Street, New York, NY 10027
Web page: http://indianeconomy.columbia.edu/
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References listed on IDEAS
Please report citation or reference errors to , or , if you are the registered author of the cited work, log in to your RePEc Author Service profile, click on "citations" and make appropriate adjustments.:
- Viktoria Hnatkovska & Amartya Lahiri & Sourabh Paul, 2012.
"Castes and Labor Mobility,"
American Economic Journal: Applied Economics,
American Economic Association, vol. 4(2), pages 274-307, April.
- Hnatkovska, Viktoria & Lahiri, Amartya & Paul, Sourabh Bikash, 2010. "Castes and Labor Mobility," Economics working papers viktoria_hnatkovska-2010-, Vancouver School of Economics, revised 22 Oct 2010.
- Kijima, Yoko, 2006. "Caste and Tribe Inequality: Evidence from India, 1983-1999," Economic Development and Cultural Change, University of Chicago Press, vol. 54(2), pages 369-404, January.
- Megha Mukim & Arvind Panagariya, 2011. "Growth, Openness and the Socially Disadvantaged," Working Papers 1113, School of International and Public Affairs, Columbia University, revised Jun 2011. Full references (including those not matched with items on IDEAS)
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Tags: Uservoice, user suggestions
Ok, the Chinesepod uservoice site is still up and running, but there used some link on the Chinesepod site - which I can't find anymore.
Although I am quite excited about the integration of Skritter, it's pretty much a response to a user suggestion (http://chinesepod.uservoice.com/pages/9762-general/suggestions/95933-add-more-character-writing-and-reading-functionality-).
Interestingly enough, topic suggestions are back to community discussions.
Not sure if your comment is appropriate? Check our Commenting Policy first.
New lesson idea? Please contact us.


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Photos are for members only!!
Peachmate is a 100% free sex site for adults looking for fun with no strings attached.
Escort ads, casual sex groups, personal ads, video chat and much more.
18 w m looking for nsa fun from the moot area. Don't mind race or culture, prefer fit or average body types


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7月 5, 2014 合計視聴数:: 5244 今日は: 1 ★★★
Catina Saigon Hotel Ho Chi Minh City, Located in the most luxurious centre of Ho Chi Minh City, Hotel Catina Saigon provides 3-star accommodation close to the city's shopping and dining area. Hotel Catina offers different, large boutique rooms that include free, high-speed internet access and city views. The hotel's services include airport transfer, same-day laundry and car rental. Guests can enjoy fine dining at Catina's Wine Bar & Restaurant where French cuisine along with a selection of wines of famous brands is served. 徒歩圏内, guests can find the Thu Thiem Ferry, Museum of Ho Chi Minh City, and the Saigon River. (Catina Saigon Hotel Ho Chi Minh City hotels booking).


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The colour I have is Icicle. It is mostly a clear sparkle. It works good for putting over a more matter lip colour. It is too sticky! Overall I don't really like it.
I love this lip gloss. It is smooth, smells and tastes pretty good, and lasts a long time. I cringe a little when I pay for anything in this product line, but this gloss is worth it!
Love Mary Kay lip gloss. Leave lips smooth. Also I find where I'm allgric to alot of products this lip stick iam not. Don't dry out my mouth and lips. Color do stay
I love these lip glosses. Not only are they glossy but they are moisturizing to the lips. They are not sticky, they are smooth and also pigmented. However, they are still sheer enough to wear over lipstick and lip liners and you can still see the color peep through.
I received the sample pack with the shades "Fancy Nancy", "Beach Bronze" "Berry Dazzle" "Shock Tart" "cafe au lait" and "red passion". They are all top notch quality and the pigmentation is excellent, especially for a lipgloss. The sparkly ones would probably need a colour underneath to match, but there's a good concentration of sparkles. would totally recommend if you can get your rep to hook you up with a discount.
Really nice lip gloss. Good variety of colours that can be used alone or over top of lipstick. I would definitely recommend it. Really good in the winter if you have chapped lips.
Best lip gloss ever! I get asked over and over, what I have on my lips
It spreads well giving smooth look that doesnt leave chunks. It makes lips feel soft and looks great. I recomend the silver moon and pink parfait colours
These reviews are the subjective opinions of ChickAdvisor members and not of ChickAdvisor Inc.


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ncnewbie Review
Perfect size!
I needed four chairs to go with two fabric print chairs in my dining room. My dining table is small and these chairs are the perfect size! The color is chocolate brown and very true to the description.
- Did the color match the color displayed onsite? Yes, I wanted chocolate brown to go with the fabric chairs I already had. The color was perfect!
- How is the quality of the product? Very sturdy and well made
- Did the description onsite accurately describe the product? Yes.
Was This Review Helpful?


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I offer a range of editorial training courses in copy-editing, proofreading and editorial project management, for beginners as well as the more experienced who wish to further their skills.
Courses can be tailored to focus on a company's specific requirements.
One-to-one tutoring is also available. In all cases, the emphasis is on enjoyable learning!
Venue: Publishing Scotland, Edinburgh
Planned for 2017 (March, June, November): Welcome to Proofreading, Welcome to Copy-editing, Further Proofreading, Further Copy-editing.
Limited Availability
Details: www.publishingscotland.co.uk
Venue: Publishing Training Centre, London; Irish Writers' Centre, Dublin
Planned for 2017: Advanced Copy-editing (London: 17 May and 21 September; Dublin date to be confirmed)
Limited Availability
Details: www.publishingtrainingcentre.co.uk
All copy-editing and proofreading courses can also be delivered in-company.


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Would u Consider dating me , based on my looks?
Most Helpful Girl
Looks are important too but I think personality is what really matters in a guy. As long as he has a nice personality and all and is respectful, committed, etc that's all I ask for. I would say you are attractive but I think you need to work on your self - esteem and your confidence and try boosting it somehow. I mean people can post all they want on here about how attractive you are. But I think the only opinion that matters is what you think of yourself, that should only matter.10|10|0
Most Helpful Guy
You look attractive. If I were gay or bi, or a woman, I would consider dating you.10|10|0
You may turn out ! Its never late dude
Nope! I'm straight as an arrow!


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For starters this isn't about you having a crush on someone you want to be with. A man crush and woman crush is a "crush" on a member of your same sex that isn't sexully based. It is more or less someone of the same sex that you idolize and look up too. Most of the time it's a famous person as well.
My man crush is Henry Rollins.
Most Helpful Guy
I know what you mean - It is probably your ideal image of man - For someone from my age group I will go Jose Mourinho


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"As Good as Private Treatment"
About: St Mary's Hospital (Newport) St Mary's Hospital (Newport) Newport PO30 5TG
Posted by Pauline Trevatt
Anything else?
Was treated as if I was a private patient excellent service from the hospital
Do you have a similar story to tell? Tell your story & make a difference ››
Response from St Mary's Hospital 4 years ago
Thank you very much for taking the time to provide us with this positive feedback. I am glad that the service you received was at the high standard that we strive to achieve.
- {{helpful}} of {{total()}} people think this response is helpful
- Was this response helpful? Yes | No
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