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Antagonistic Gcn5-Hda1 interactions revealed by mutations to the Anaphase Promoting Complex in yeast
© Islam et al; licensee BioMed Central Ltd. 2011
Received: 20 January 2011
Accepted: 8 June 2011
Published: 8 June 2011
Histone post-translational modifications are critical for gene expression and cell viability. A broad spectrum of histone lysine residues have been identified in yeast that are targeted by a variety of modifying enzymes. However, the regulation and interaction of these enzymes remains relatively uncharacterized. Previously we demonstrated that deletion of either the histone acetyltransferase (HAT) GCN5 or the histone deacetylase (HDAC) HDA1 exacerbated the temperature sensitive (ts) mutant phenotype of the Anaphase Promoting Complex (APC) apc5 CA allele. Here, the apc5 CA mutant background is used to study a previously uncharacterized functional antagonistic genetic interaction between Gcn5 and Hda1 that is not detected in APC5 cells.
Using Northerns, Westerns, reverse transcriptase PCR (rtPCR), chromatin immunoprecipitation (ChIP), and mutant phenotype suppression analysis, we observed that Hda1 and Gcn5 appear to compete for recruitment to promoters. We observed that the presence of Hda1 can partially occlude the binding of Gcn5 to the same promoter. Occlusion of Gcn5 recruitment to these promoters involved Hda1 and Tup1. Using sequential ChIP we show that Hda1 and Tup1 likely form complexes at these promoters, and that complex formation can be increased by deleting GCN5.
Our data suggests large Gcn5 and Hda1 containing complexes may compete for space on promoters that utilize the Ssn6/Tup1 repressor complex. We predict that in apc5 CA cells the accumulation of an APC target may compensate for the loss of both GCN5 and HDA1.
Eukaryotic genetic information is packaged into chromatin, a highly organized and dynamic protein-DNA complex. The fundamental unit of chromatin, the nucleosome, is an octameric structure composed of two copies of each of the four core histones (an H3/H4 tetramer and two H2A/H2B dimers), surrounded by approximately 146 bp of DNA [1, 2]. Many cellular processes depend on modifications of both DNA and histones within nucleosomes [3, 4]. Modification of chromatin by histone acetyltransferases (HATs) and histone deacetylases (HDACs) play key roles in transcriptional regulation [5–9]. Post-translational acetylation of the highly conserved lysines within the N-terminal tail domains of the core histones is strongly correlated with transcriptional activation [5, 10]. Although the precise mechanisms by which histone acetylation alters transcription are poorly understood [9–12], there is tremendous pressure to understand these mechanisms, as impaired histone modification is linked to many disease states [13].
The study of HAT and HDAC recruitment to promoters and their interaction with activators and repressors are essential for a better understanding of gene regulation. HATs and HDACs modify histones enzymatically throughout the genome [14]. Histone acetylation potentially regulates transcription by manipulating the higher-order folding properties of the chromatin fiber [15–17]. General control nonderepressible 5 (Gcn5) [18] was the first identified HAT and exists as the catalytic subunit in multiple high molecular weight complexes in yeast, including SAGA (Spt-Ada-Gcn5-Acetyltransferase), SLIK (SAGA-like), ADA (transcriptional ADAaptor), and the smaller HAT-A2 complex [19–23]. As part of the evolutionarily conserved SAGA complex, Gcn5 predominantly acetylates nucleosomal H3 lysines K9, K18, and K27 [24]. Defects in human SAGA subunits are associated with multiple disorders, including neurological diseases and aggressive cancers [25, 26]. Gcn5 is a direct target for recruitment by transcriptional activators in vitro [27, 28] and in vivo [29], which results in the acetylation of nearby histones [10]. Elongation of the transcripts initiated by Gcn5-containing complexes is carried out by the Elongator complex, which utilizes Elp3 as its primary HAT [30, 31]. Cell cycle specific roles for Gcn5 have been reported, as recruitment of Gcn5 to a set of genes that are expressed in late mitosis requires SWI/SNF remodelling activity [32]. Furthermore, Gcn5 displays an overlapping pattern of localization with several HDACs [24, 33, 34]. Acetylation microarrays have shown that Rpd3 and Hda1 are the principal HDACs in yeast, affecting numerous promoters throughout the genome with little overlap between promoters [10, 35]. Hda1, an evolutionary conserved HDAC, which deacetylates mainly histones H2B and H3 [36, 37], is recruited to promoters via utilization of different Tup1/Ssn6 domains [38–40], resulting in local deacetylation. HDAC recruitment may form a positive feedback loop to repress transcription locally and facilitate the spreading of Tup1 into adjacent regions [41]. Tup1-mediated repression requires the deacetylation of histones within promoters [42–44], which may require direct recruitment of HDACs [36, 45, 46]. Overall, the mechanisms of Tup1/Ssn6-mediated transcriptional repression can be classified into 3 classes: (i) direct interaction with the activator; (ii) repression by changing chromatin structure; and (iii) interaction with the general transcription machinery [47, 48]. It appears that different groups of genes have developed different strategies to utilize Tup1/Ssn6, enabling it to function as a global repressor.
Our work has linked the Anaphase Promoting Complex (APC), an evolutionarily conserved 13 subunit complex in yeast that is critical for mitotic progression and G1 maintenance [49–52], with chromatin assembly and histone acetylation through genetic interactions with chromatin assembly factor (CAF), HAT and HDAC mutants [53–57]. The APC is a ubiquitin-protein ligase (E3) that targets proteins that block the initiation of anaphase (Pds1) and mitotic exit (Clb2) for degradation. Various regulators govern APC activity in positive and negative manners, from phosphorylation and transcriptional control of APC subunits, to sequestration of APC activators [58–63]. For example, protein kinase A (a complex of Bcy1, Tpk1, Tpk2 and Tpk3) and Mad2 inhibit APC activity through phosphorylation and subunit sequestration, respectively. Activating phosphorylation is supplied by the polo-like kinase (Cdc5) and Cdc28. Furthermore, Cdc20, inhibited by a Mad2-dependent mechanism, binds and activates the APC to promote the metaphase/anaphase transition, while Cdh1, another APC-binding partner, drives APC-dependent mitotic exit. Previous studies by our group have expanded the APC's functional repertoire by showing that the mutant APC subunit allele, apc5 CA [54], genetically interacted with deletions of the HAT encoding genes GCN5 and ELP3 [57]. Strains harboring the apc5 CA gcn5 Δ or the apc5 CA elp3 Δ mutations had severely restricted growth at elevated temperatures compared to the single mutants. This interaction implies that the APC and these HATs positively interact, but a negative feedback loop appears apparent, as G1-specific Gcn5 instability was reduced in APC mutant cells. An additional synergistic genetic interaction between hda1 Δ and apc5 CA was also observed, suggesting that the APC interacts positively with the HDAC Hda1 [57]. The study presented here focuses on a novel antagonistic relationship between gcn5 Δ and hda1 Δ that is revealed in apc5 CA , but not APC5 cells. We provide further evidence that the APC works with multiple histone modifiers to drive cell cycle progression.
gcn5 Δ/hda1 Δ interactions revealed in an APC mutant background
To examine whether Hda1 positively interacted with the APC, we expressed galactose driven HDA1 carrying a C-terminal HA tag (GAL pro HDA1-HA) at low levels in WT, apc5 CA and gcn5 Δ cells by using glucose as a carbon source (Figure 1B). Recently, we observed that mRNA levels of GAL pro GCN5-HA were elevated 100-fold when grown on 2% glucose and 900-fold when grown on 2% galactose [57]. However, Gcn5-HA protein expression remained low even though GCN5-HA mRNA was 100-fold elevated when grown on 2% glucose. As shown with GCN5 [57], low-level GAL pro HDA1-HA expression improved apc5 CA growth (Figure 1B). This is not necessarily a general feature of histone modifying proteins, as deletion or overexpression of the HAT HPA2 had little effect on apc5 CA cells (Figure 1B) [57]. Although the yeast Hpa2 has not yet been shown to acetylate histones in vivo, a bacterial acetyltransferase that does acetylate eukaryotic histones is most closely related to Hpa2, and Hpa2 does acetylate H3 in vitro [65, 66]. Moreover, Hpa2 appears to be active, as overexpression reduces growth of gcn5 Δ cells, whereas expression on glucose improves growth of apc5 CA cells (Figure 1B).
A further connection between Gcn5 and Apc5 was observed by the rescue of GAL pro APC5-HA overexpression toxicity by deletion of GCN5 (Figure 1B). It is unlikely that Apc5 protein levels induced from the GAL promoter are compromised in gcn5 Δ cells, as expression of HPA2 and HDA1 from the GAL promoter reduces gcn5 Δ growth. Overexpression of APC5 from the CUP1 promoter also reduced yeast replicative lifespan [60]. Rescue of APC5 toxicity by GCN5 deletion is consistent with our recently proposed hypothesis that Gcn5 is required for APC activity, and may provide an explanation as to why GCN5 [57] and HDA1 (Figure 1B) overexpression is toxic, considering that overabundance of Apc5 is detrimental to cells.
Next, we asked whether mutations to APC5 influenced acetylation of histone H3 lysine 9 or 14 (H3K9/14) in gcn5 Δ and hda1 Δ cells. Gcn5 appears to play a greater role on H3K9, compared to H3K14, whereas loss of HDA1 results in increased acetylation of both H3K9 and H3K14 (Figure 1C). The apc5 CA background did not change the acetylation status of H3K9/14 in gcn5 Δ or hda1 Δ cells, suggesting the apc5 CA background may be revealing an effect other than global histone H3 acetylation. H3K9Ac was reduced in gcn5 Δ, apc5 CA gcn5 Δ and apc5 CA gcn5 Δ hda1 Δ cells, but not in gcn5 Δ hda1 Δ cells. The ability to acetylate H3K9 in gcn5 Δ hda1 Δ cells indicates that on a global level, other HATs can use H3K9 as a substrate. However, at the gene level, deletion of GCN5 was previously shown to reverse histone hyperacetylation at the PHO5 promoter when HDA1 was deleted [67]. Therefore, we tested whether transcript levels are influenced by apc5 CA in gcn5 Δ or hda1 Δ cells.
The apc5 CA allele increases transcript levels in hda1 Δ cells
Increased PDS1 transcripts in apc5 CA hda1 Δ cells correlates with increased promoter acetylation
Consistent with our observations that transcript levels of BCY1 and PDS1 increase in apc5 CA hda1 Δ cells, we detected increased BCY1 and PDS1 promoter acetylation in these cells, specifically at 37°C. Transcript levels and promoter acetylation are both increased with PDS1 at 37°C in apc5 CA hda1 Δ cells. However, we note some differences in the patterns observed. For example, BCY1 transcripts are not elevated in apc5 CA hda1 Δ cells at 37°C while promoter acetylation is. This may reflect the complex nature of the factors assembled at promoters that is not addressed in this study.
Gcn5 promoter occupancy increases in the absence of Hda1
Next we asked whether promoter occupancy by Gcn5 correlated with gene expression and promoter acetylation. GAL pro GCN5-HA was induced in gcn5 Δ and gcn5 Δ hda1 Δ cells so that the only Gcn5 expressed was HA tagged. gcn5 Δ cells expressing GAL pro GCN5-HA grew like WT (data not shown), and were considered the WT control for this experiment. ChIP was performed in lysates prepared from these cells. Control ChIPs were performed using untagged lysates (data not shown), and reactions without antibody, neither of which produced PCR products. Primers against the 5', middle, and 3' regions of CDC20 demonstrated that Gcn5-HA recruitment was most prominent at the promoter and was reduced 5' to 3' (data not shown). We found that in HDA1 cells expressing GCN5-HA, very little Gcn5-HA was present at the promoters tested compared with the RDN1 promoter (Figures 4C and 4D). In hda1 Δ GCN5-HA cells, however, increased Gcn5-HA promoter recruitment was observed. The increases observed were slight except for the CDC20 promoter. Promoter acetylation also increased in hda1 Δ cells, consistent with increased recruitment of Gcn5. These observations present the possibility that i) increased promoter H3K9/14 acetylation in hda1 Δ cells is due to increased Gcn5-HA promoter recruitment; and/or ii) Hda1 may block access of Gcn5 to promoters.
Tup1 occludes Gcn5 promoter occupancy
To distinguish between these possibilities, we predicted that if Tup1 and Hda1 work together, then deletion of TUP1 in apc5 CA cells should have the same synergistic effects as an HDA1 deletion. Our results show that deletion of TUP1 impairs the apc5 CA phenotype (Figure 6C), similar to an hda1 Δ mutation. This suggests that both Hda1 and Tup1 perform a function that is beneficial to APC activity. However, it does not necessarily indicate they work together to perform this task.
Hda1 and Tup1 likely interact at promoters, which can be inhibited by Gcn5
Novel Gcn5/Hda1 antagonistic functional interactions are revealed when APC activity is compromised
The work presented here provides evidence to support a model in which the HAT Gcn5 and the HDAC Hda1 functionally interact at promoters to determine transcriptional readouts (Figure 8). In otherwise WT cells, mutations to GCN5 or HDA1 do not create significant growth defects, whereas in apc5 CA cells, these same mutations produce severe ts growth defects (Figure 1A). The focus of this study was to characterize an antagonistic functional gcn5 Δ/hda1 Δ interaction revealed in the apc5 CA background, as the severe apc5 CA gcn5 Δ and apc5 CA hda1 Δ ts defects are suppressed in apc5 CA gcn5 Δ hda1 Δ cells. Growth phenotypes associated with deletion of GCN5 have been shown in two separate Synthetic Genetic Array (SGA) genome-wide screens to be suppressed by deletion of HDA1 [76, 77]. However, spot dilution analysis of the gcn5 Δ and hda1 Δ cells on YPD did not reveal any phenotypes [76], as shown in our study (Figure 1A). Thus, the gcn5 Δ hda1 Δ antagonistic interaction is not apparent under normal growth conditions, such as on YPD, but under conditions imposed by the SGA screen (selective media, for example), the antagonistic interaction can be exposed. The influence of the apc5 CA allele on this interaction was investigated. The apc5 CA allele had little effect on global histone H3 acetylation status in gcn5 Δ and hda1 Δ cells, but did cause the increase of BCY1 and PDS1 transcripts in hda1 Δ cells (Figures 1C, 3). Both Bcy1 and Pds1 proteins antagonize APC activity and may be involved in the enhanced growth defect when APC is mutated. Therefore, in apc5 CA cells, it may be the inappropriate expression of inhibitory transcripts that are paramount to synergistic apc5 CA gcn5 Δ and apc5 CA hda1 Δ phenotypes.
A molecular mechanism explaining the Gcn5/Hda1 interaction likely involves competition for Tup1 binding. We observed that in cells lacking HDA1 or TUP1, Gcn5 recruitment at our tested promoters was increased (Figures 4 and 6). On the other hand, deletion of GCN5 increased Hda1-Tup1 physical interactions at promoters (Figure 7). A competition between Hda1 and Gcn5 for Tup1 binding is a possibility worth considering, as both Hda1 and Gcn5 have been shown to physically interact with Tup1 [36, 73–75]. However, in gcn5 hda1 Δ cells this mechanism would not be possible. In addition to the accumulation of Gcn5 in apc5 CA cells, we observed that Elp3 also accumulates when the ubiquitin system is compromised (Figures 4A, B). We previously demonstrated that gcn5 Δ and elp3 Δ deletions impair apc5 CA defects, that GCN5 and ELP3 overexpression stalls the cell cycle in G1, and that Gcn5 G1-specific instability is reversed in APC mutants [57]. Thus, when apc5 CA is combined with gcn5 Δ hda1Δ, an APC target likely accumulates that creates novel transcripts that allow bypass of the severe ts defects observed in the double mutants. Elp3 is an attractive candidate since it is involved in elongating transcripts initiated by Gcn5 containing complexes [31]. A global transcript analysis is likely required to follow this further. Our previous work suggests that the apc5 CA phenotype is sensitive to global transcript levels [57].
Hda1-dependent occlusion of Gcn5 from promoters requires Tup1
Several reports describe the recruitment of the Tup1/Ssn6 repressor complex to DNA via interactions with multiple partners [41, 48, 68]. Once recruited, Tup1 then contacts H3 and H4 N-terminal tails [78]. Mechanisms employed to recruit Tup1/Ssn6 to promoters by the various individual interacting partners appears to be complex, seems to vary, and may have overlapping roles. Gcn5-HA recruitment to the tested promoters was increased in hda1 Δ, tup1 Δ and ssn6 Δ cells (Figure 6; data not shown), indicating that the interaction of Hda1 with the Tup1/Ssn6 repressor complex is necessary to block access to Gcn5. Tup1 and Hda1 did indeed co-immunoprecipitate while bound to the same promoters, as shown by sequential ChIP (Figure 7). We find it unlikely that Tup1 and Hda1 are simply associating independently at adjacent sequences within the 200-basepair DNA PCR fragment, since they have been shown to interact previously [34], and are part of large complexes [19–23], but we cannot discount this possibility. However, we observed that in gcn5 Δ cells, Hda1-Tup1 association increased at some promoters (PDS1 and BCY1), suggesting Gcn5 opposes complex formation. The mechanism of action that Gcn5 uses to block Hda1-Tup1 association remains unclear. Previous reports indicating that Tup1 is capable of recruiting and interacting with Gcn5/SAGA at promoters [73–75] suggest it is possible that Gcn5 and Hda1 may compete for Tup1 interaction. The scenario for recruiting either Gcn5 or Hda1 would differ, implying other proteins may be involved in deciding whether Gcn5 or Hda1 gain access. We were unable to observe complex formation between Gcn5-TAP and Hda1-HA in whole cell lysates (data not shown), indicating possible exchange of Gcn5 and Hda1 at Tup1 complexes does not require Gcn5-Hda1 association. It is also possible that Gcn5-Hda1 physical interactions are transient and promoter specific, therefore may not be detectable using the methods applied here. Nonetheless, support for our model was provided by reports describing recruitment of Gcn5 to promoters by the Tup1/Ssn6 complex under osmotic stress conditions [40, 74], indicating that Tup1/Ssn6 may be a transcriptional activator under certain conditions.
The results presented in this manuscript provide evidence for a complex network of interactions between a mitotic/G1 cell cycle regulator (the APC), and antagonistic interplay between a HAT (Gcn5), and an HDAC (Hda1). Gcn5 is known to function during mitosis [32, 57, 79, 80]. Data on the role Hda1 plays in cell cycle progression is limited, but Hda1 may provide some function to ensure histones are deacetylated prior to passage through mitosis [81]. It is noteworthy that Gcn5 and Hda1 expression is temporally regulated during the cell cycle (microarray data compiled at Saccharomyces Genome Database), providing insight into how the potential competition for Tup1 binding could be regulated. APC mutations cause cell cycle progression to stall during mitosis, potentially skewing the equilibrium between Gcn5 and Hda1 promoter recruitment if the cell cycle does indeed influence Hda1 and Gcn5 recruitment. Future work will focus on identifying the molecular mechanisms regulating how cell cycle progression influences chromatin dynamics. Chromosome synthesis and segregation defects are widely associated with human disease, thus continued work into furthering our understanding of this process is vital.
Media, yeast strains, plasmids and general methods
Yeast strains used in this study
Relevant genotype
MATα ade2 his3 Δ200 lys2 Δ201 ura 3-52
MATa ade2 his3 Δ200 lys2 Δ201 ura3-52
MATa his3 Δ1 leu2 Δ met15 Δ ura3 Δ tup1 Δ::kanMX6
MATa his3 Δ1 leu2 Δ met15 Δ ura3 Δ ssn6 Δ::kanMX6
MATa ade2 his3 Δ200 lys2 Δ201 ura3-52
MAT(?) ade2 his3 leu2 lys2(?) ura3 apc5 CA -PA::His5 + tup1 Δ::kanMX6
This study
MAT(?) ade2 his3 leu2 lys2(?) ura3 hda1 Δ::kanMX6
MAT(?) ade2 his3 leu2 lys2(?) ura3 apc5 CA -PA::His5 + hda1 Δ::kanMX6
MAT(?) ade2 his3 leu2 lys2(?) ura3 gcn5 Δ::kanMX6
This study
MAT(?) ade2 his3 leu2 lys2(?) ura3 apc5 CA -PA::His5 + gcn5 Δ::kanMX6
This study
MAT(?) ade2 his3 leu2 lys2(?) ura3 gcn5 Δ::kanMX6 hda1 Δ::kanMX6
This study
MAT(?) ade2 his3 leu2 lys2(?) ura3 apc5 CA -PA::His5 + gcn5 Δ::kanMX6 hda1 Δ::kanMX6
This study
MATa his3 Δ1 leu2 Δ met15 Δura3 Δ rpn10 Δ::kanMX6
MATa his3 Δ1 leu2 Δ met15 Δ ura3 Δ GCN5-TAP::HIS3
as YTH1235, with GCN5-TAP::HIS3
as YTH5, with tup1 Δ::kanMX6
This study
as YTH5, with ssn6 Δ::kanMX6
This study
MAT(?) ade2 his3 leu2 lys2(?) ura3 gcn5 Δ::kanMX6 ssn6 Δ::kanMX6
This study
MAT(?) ade2 his3 leu2 lys2(?) ura3 gcn5 Δ::kanMX6 tup1 Δ::kanMX6
This study
MATa his3 Δ1 leu2 Δ met15 Δ ura3 Δ APC5-TAP::HIS3 rpn10 Δ::kanMX6
This study
Plasmids used in this study
Plasmid name
Markers/Integrated genes
GAL pro -APC5-HA
2μ GAL10 pro -APC5-HA URA3
GAL pro -GCN5-HA
2μ GAL10 pro -GCN5-HA URA3
GAL pro -HDA1-HA
2μ GAL10 pro -HDA1-HA URA3
W. Xiao/ResGen
GAL pro -HPA2-HA
2μ GAL10 pro -HDA1-HA URA3
W. Xiao/ResGen
2μ CUP1 pro -TUP1 URA3
2μ CUP1 pro -TUP1 URA3
Primers generated for the Northern analysis
Forward primer
Reverse primer
Reverse transcriptase PCR (rtPCR)
Primers generated for the ChIP analysis
Forward primer
Reverse primer
Chromatin immunoprecipitation (ChIP)
ChIP was performed essentially as described elsewhere [82, 83] with the following modifications: DNA fragment size achieved by sonication was 500-1000 bp, and 100 μg of protein lysate was used for each IP. Protein concentration was determined by a Bradford protein assay. 5 μg of ChIP grade rabbit polyclonal anti-acetyl-H3K9/14 (Upstate Biotechnology), rabbit polyclonal anti-H3 (Abcam), rabbit polyclonal HA antibody (Abcam), and rabbit polyclonal GST antibody (Abcam) were used for IP. One-tenth of the total volume of lysate was used as input for each sample. Sequential ChIP was performed as previously described [84]. In sequential ChIP experiements, the immune complexes were eluted by incubation for 30 minutes at 37°C in 10 mM DTT. After centrifugation, the supernatant was diluted 25 times with ChIP dilution buffer (1% Triton X-100, 2 mM EDTA, 150 mM NaCl, 20 mM Tris-HCl [pH 8.1]) and subjected again to ChIP using a different antibody. In this experiment, HA antibody was applied first, followed by GST antibody. Cross-linking of the immune complex was reversed by adding NaCl to a final concentration of 0.3 M and incubated overnight at 65°C. Samples were treated first with 1 μg/μl RNaseA (Millipore [formerly Upstate]) for 30 minutes at 37°C, followed by 1 μg/μl proteinase K (Millipore [formerly Upstate]) at 45°C for 1 hour. DNA was purified by chromatography on QIAquick columns, and eluted with elution buffer (PCR purification kit, Qiagen). PCR was performed for semiquantitative determination by standard end point PCR. 1 μl DNA was used for PCR, and the reaction continued to the predetermined mid-linear range for each primer set. The end point PCR product was resolved on a 1% agarose gel and visualized by ethidium bromide. Two independent experiments were performed for each ChIP. The gel bands from each experiment were analyzed by ImageJ, and the means and standard error were plotted for graphical representation. For time course experiments, 200 ml cultures were induced at a final concentration of 4% galactose. Samples (20 ml) were immediately removed, and again after 1, 3 and 5 hours. The 20 ml samples were in duplicate for Western and ChIP analysis.
AI was supported by Post-Doctoral Fellowships from the Saskatchewan Health Research Foundation (SHRF), and from the Canadian Institutes for Health Research-Regional Partnership Program (CIHR-RPP). ELT was supported by Graduate Scholarships from the College of Graduate Studies at the U of S. Deletion mutants and plasmids were kindly provided by Dr. W. Xiao (University of Saskatchewan). We thank members of the Harkness lab, Ata Ghavidel and Spike Postnikoff, for careful reading of the manuscript and for providing insightful suggestions. Funding was generously provided to TAAH through a CIHR Operating Grant and a New Investigator Award from the Canadian Foundation for Innovation (CFI).
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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UNSAFE, ineffective, careless, unresponsive and poorly-led is how the national health watchdog has described Old Town nursing home, Princess Lodge Care Centre.
The Curie Avenue centre, which is run by national firm Life Style Care, was inspected on July 10 and found to be failing to meet all five of the standards it is expected by the Care Quality Commission.
Warning notices, a last resort used by the CQC for breaches in regulations, have been served on the home, which was given until last Saturday to file an action plan for transforming its practises.
The inspection was unannounced and inspectors said it was carried out after they had concerns about the care people were receiving.
Inspectors said: "The service was not safe. There were not sufficient numbers of qualified, skilled and experienced staff to carry out the regulated activities.
"People who used the service were not protected from the risk of abuse because the provider had not taken reasonable steps to identify the possibility of abuse and prevent abuse from happening.
"We found people were not always supported to eat or drink enough to maintain their health. Staff had not always received training to meet the needs of people living at the home.
"Care did not always take account of people's individual preferences and did not always respect their dignity.
"People were not always spoken to in a way that was respectful or caring.
"People that had lost significant amounts of weight had not been referred to a dietician and care records did not always show the most up-to-date information on people's needs and risks."
Among the evidence presented by inspectors, one person had reportedly been asking for a shower since the previous day, but staff said they were too busy.
The person said: "I feel wet and dirty."
An interaction between two service users in one of the lounges on-site, which escalated into shouting, left both parties distressed, yet two care workers entered on three occasions and ignored them.
Two people remained in bed all day because there were not enough staff to support them, and others remained in bed after lunch because they were not assisted with dressing.
"These situations restricted people's liberty in a way that may amount to deprivation of liberty but without the authorisation to do so," said the report.
Some people did not have call bells in their room and there was no evidence to suggest why this was, according to the inspectors.
Those who did not have call bells or could not use them would reportedly scream if they needed anything, but even by this method they were not responded to in a timely manner.
One service user was identified as losing more than 16 per cent of their body weight over a six-month period.
There was no evidence a GP had ever assessed this service user in relation to the extreme weight loss, despite the claims made by staff.
This user wasleft drinks and biscuits on a side table out of their reach on the other side of the room.
A spokesman for Swindon Council, which was contacted after the report was published, said: "We are working jointly with the CQC to make sure the care home reaches the required standard, and as part of this we are making random and frequent visits to check that improvements are being made in line with the requirements of the CQC report.
"These visits can last for several hours and what we find is shared with both the CQC and the operator of the care home."
Lisa Mitchell, the regional director for Life Style Care, said: "The management at Princess Lodge Care Centre acknowledge there have been serious shortfalls in the standards of care and support of the residents in the home.
"Significant progress has already been made since the inspection in July, including the appointment of a new permanent home manager.
"There is a programme in place to improve the outcomes that residents at Princess Lodge may expect.
"Substantial developments in the support systems and structures are being made and the staff team are committed to ensuring that consistent positive progress is maintained."


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NXP Semiconductors introduces world's first fully integrated HDMI 1.3 Interface Conditioning chips
IP4777CZ38 and IP4778CZ38 deliver Advanced HDMI Interface Conditioning in a single chip
(PresseBox) (
NXP Semiconductors, the independent company founded by Philips, today announced the industry's first fully integrated Interface Conditioning chips for the HDMI(TM) 1.3 port. The new family of chips deliver 8kV of ESD protection (according to the IEC61000-4-2 standard), as well as DDC buffering, hot plug control, an enable signal for HDMI port switching and CEC ringing prevention. The NXP IP4777CZ38 chip, designed for sources such as DVD players, and the IP4778CZ38 chip, designed for sinks such as TV sets, also offer ultra-low line capacitance to ensure high signal integrity for the latest video formats such as 1080p.
NXP Semiconductors' IP4777CZ38 and IP4778CZ38 HDMI Interface Conditioning chips each integrate 30 to 50 discrete components, helping designers to reduce PCB space, design time and total system cost, and enabling manufacturers to boost their board assembly capacity by reducing throughput time.
"Many customers are asking for a reduction of device count on the board," says Kai Neumann, product line manager for Integrated Discretes at NXP Semiconductors. "By offering a fully integrated HDMI 1.3 Interface Conditioning solution with an integration level of 30 to 50 components, we enable our customers to have their newest designs faster in the market with a lower assembly bill. The end customer will benefit from the high IEC ESD protection level and the option to connect long HDMI cables to audio-visual equipment."
Integrated functions include for both the IP4777CZ38 and IP4778CZ38 include:
- ESD protection of TMDS, Hot Plug, DDC and CEC lines according the strict IEC standard. Since HDMI is a hot-plug interface, ESD protection becomes much more important with a new series of mobile HDMI devices generating several plug events each day. IP4777CZ38 and IP4778CZ38 protect the sensitive HDMI system chip against ESD pulses according to the tough IEC61000-4-2, level 4 standard, since this standard gives the most realistic representation of a person touching an interface under real-life conditions.
- DDC capacitive decoupling and EMI filtering between system side and HDMI connector side and buffering to drive cables with high capacitive load (>700pF). This protects TV set manufacturers from unjustified returns due to end customers using out-of-spec cables.
- Very high diode switching speeds (nsec) and low line capacitances of 0.7pF to ground and 0.05pF between the channels to ensure signal integrity, providing easy and relaxed board layout to enable designers to improve time-to-market of their new platforms.
- Hot plug control for direct connection to system GPIO optimized for sources (IP4777CZ38) or sinks (IP4778CZ38).
- CEC ringing prevention through slew rate limiter back-drive protection
- 4 bi-directional 3.3V to 5V level shifters for I2C, CEC and HPD signals.
- Enable signal for DDC and hot plug for multiplexing and back-drive protection.
Availability The NXP IP4777CZ38 and IP4778CZ38 HDMI Interface Conditioning chips are available immediately worldwide. Comprehensive documentation and technical support is available from NXP, including HDMI verification of our devices on customer boards. Support is provided on a multi-site and multi-region level for OEMs that work with ODM/EMS partners, or have several manufacturing sites.
Further information on the IP4777CZ38 and IP4778CZ38 HDMI Interface Conditioning chips are available at
NXP Semiconductors Germany GmbH
Stresemannallee 101
+49 (40) 56132280
Akhila Deshpande
+44 (20) 88460789
Freek van Vlerken
+31 (40) 27-25181
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text:
Coghead Pursues Platform Strategy With Launch Of Affiliates Program
Every company in Silicon Valley wants to become a platform for other companies to build cool stuff on top of. It is the easiest way to attract customers. Coghead—the DIY, Web-based, business-app builder—is no different. Today it is publicly launching its charter affiliates program, whereby software developers can create their own enterprise apps using Coghead and then resell them… Read More


text:
Wolves Team News
Glenn Hoddle makes two changes to todays line up, Jeremie Aliadiere coming in for Tomasz Frankowski. Paul Ince also makes a surprise return coming in for Denes Rosa. Rob Edwards takes over from eth injured Jody Craddock.
Starting Line Up
Postma, Ross, Edwards, Lescott, Naylor, Ince, Davies, Anderton, Aliadiere, Cort, Miller
Subs: Oakes, Kennedy, Rosa, Ricketts, Frankowski.
Hull: Myhill, L.Cort, Delaney, Andrews (capt), Elliott, France, Green, Parkin, Noble, Thelwell, Rogers. Subs: Duke, Paynter, Fagan, Duffy, Welsh.


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Research by NM Incite reveals some notable but not terribly shocking data regarding why people engage with social media.
From the highlights:
Not surprisingly, the top drivers of social media use among social networkers are keeping in touch with family and friends (89% and 88%, respectively) and finding new friends (70%). Another driver of use is the desire to view and contribute to reviews of products and services as 68 percent of social media users go to social networking sites to read product reviews and over half use these sites to provide product feedback, both positive and negative. Other top reasons social media users engage in social networking include entertainment (67%), as a creative outlet (64%), to learn about products (58%), and to get coupons or promotions (54%).
I am surprised people use it more to praise a product than to bury one, given often I see people airing out their grievances about products on Twitter. On the other hand, people do not generally like the Facebook pages of a company that they dislike.


text:
What is the 'Mean Return'
The mean return, in securities analysis, is the expected value, or mean, of all the likely returns of investments comprising a portfolio. It is also known as "expected return".
2. In capital budgeting, it is the mean value of the probability distribution of possible returns.
BREAKING DOWN 'Mean Return'
Mean returns attempt to quantify the relationship between the risk of a portfolio of securities and its return. It assumes that while investors have different risk tolerances, rational investors will always seek the maximum rate of return for every level of acceptable risk. It is the mean, or expected, return that investors try to maximize at each level of risk.


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How to set the Encoding Type for SQL Manager in PrestaShop 1.5
Written by Scott Mitchell
Views: 3,034
Published: Oct 8, 2012
Comments: 0
Our last article for the Working with SQL Manager course will cover how to change the database encoding type, either UTF-8 or ISO-8859-1. Follow the steps below to learn how to modify the encoding type in PrestaShop 1.5
Setting the SQL Manager Encoding type in PrestaShop 1.5
- Log into your PrestaShop 1.5 dashboard.
- Using the top menu bar, hover over the Advanced Parameters tab and click on the SQL Manager option from the menu.
- You are now on the SQL Manager page. Look to the lower section of the page entitled Settings. From there, you will see the Select your encoding file by default field.
- The dropdown offers two selection:
UTF-8 Known as Unicode, it is the current standard. It encompasses all known languages. ISO-8859-1 Older standard, being replaced by UTF-8. Generally for Western European languages.
- Once you have selected the encoding type, click on the green Save button to finalize any changes.
Continued Education in Course 215: Working with SQL Manager in PrestaShop 1.5
You are viewing Section 5: How to set the Encoding Type for SQL Manager in PrestaShop 1.5
‹Section 4: How to delete a custom SQL Query in PrestaShop 1.5
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Parution de quatre Leçons inaugurales du Collège de France
Four new inaugural lectures in the Collège de France's Leçons inaugurales series are now available in full text: lecture no. 213, Le théâtre n'existe pas [Theatre does not exist] by Jacques Nichet, lecture no. 214, Mobiliser le savoir pour éradiquer la faim [Mobilise knowledge to eradicate hunger] by Ismail Serageldin, lecture no. 215, L'art survivra à ses ruines [Art will survive its ruins] by Anselm Kiefer and lecture no. 216, L'énergie : stockage électrochimique et développement durable [The electrochemical storage of energy and sustainable development] by Jean-Marie Tarascon. At the Collège de France the leçon inaugurale is the first lecture given by a new professor. It is the chance for the new arrival to situate his work and teaching in relation to his predecessors and the most recent research developments. The lectures are now published in both paper and electronic versions. Each electronic issue is composed of a presentation of the professor by Collège de France's administrator, the text of the lecture and a video recording of the lecture.


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Online Article Directory!
Get the Help of Realty Agents in Kolkata
For quite an extended time the real estate in Kolkata is undergoing nice development. This development is obvious each within the commercial and residential sectors. So as to catch up with the rising trends of the real estate developers in India, the Kolkata builders too are welcoming the concept of realty constructions of contemporary concept. Moreover the quantity of individuals wanting property in Kolkata has risen to an excellent extent. Each issue of convenient living like amenities, locations etc are being given utmost importance before purchasing the residential property in Kolkata. Hence the consumers realize property buying a possible choice in Kolkata. Differing kinds of realty developers like Diamond Group, DLF Group etc have realized the importance of Kolkata real estate; hence, they're launching their residential property in Kolkata that is receiving nice appreciation.
The spurt in realty property in Kolkata and real estate firms in Kolkata is attributable to this very reason.
People have realized the nice worth of property during this city; hence, opt to obtain or sell their property in expectation of lucrative deals. However the complete method of buying and selling of properties become quite an ordeal if there's nobody to guide you thru the method. During this huge, dangerous world of property and realty, you would possibly not remember of the various intricacies that you'd need the support and advice of a realty agent. These skilled are like experienced people that possess the required experience want to guide you thru the method and build it less cumbersome.
Those people that have undergone the difficulty of experiencing the ordeal of buying and selling properties savvy necessary it's to own a real estate agent by your aspect.
For example, if you would like to purchase for an rich property in Kolkata in one of the luxurious space of the town then nothing may be higher than Belvedere, Southern Avenue, Salt Lake, Ballygunge etc. Even Rajarhat is witnessing a robust influx of manpower and is developing steadily. However so as to remember of the special options of various areas you ought to recognize why they're thus necessary. So, the simplest issue you'll do is take the help of a real estate agent who would guide you sufficiently through the method.
Those people that are probing for homes for sale in Kolkata ought to contemplate seeking the help of an agent. Usually, as a result of often individuals build the error of going for an uninformed plan while not taking the benefit of a real estate agent. Obtain when someday they notice what was the purpose of taking most pain? Moreover once you are utterly inexperienced during this field it's quite probably that you simply would face a lot of issue in operating without a real estate agent. Why would you're taking the difficulty of doing all analysis on-line so as to work out what steps you wish to require once you pays someone to try to all of this stuff?
If it's the issue of cash that is coaxing you within the different direction you ought to recognize that while not an agent you'll waste plenty of time, effort while not deriving any correct results. Thought you would possibly ought to pay your agent some cash however then it's definitely worth the expense. He would make sure that the system is totally discovered in order that you are doing not ought to undergo any reasonably ordeal. Moreover the rates charged by the real estate agents aren't exorbitant thus you'd have any downside in paying them notwithstanding what your budget is.
Vinay Jain - About Author:
PropTiger is an independent real estate advisor with a pan-India presence. We aspire to be your first port of call if you want to buy a property in Kolkata.
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The better the relationship, the less likely that there will be any concealed information or errors in filing for debt relief options.
New Orleans, LA (PRWEB) February 23, 2013
CTR's New Orleans tax attorney is pleased to announce the start of a new federal tax debt relief investigation program that will be implemented by tax experts in Louisiana and nationwide. This new program will better serve taxpayers in debt to the IRS. The main goal of this debt relief initiative is to bring taxpayers into good standing with the IRS regardless of the cause of their tax problems.
"Taxpayers in debt to the IRS are often under a lot of stress," said a CTR spokesperson. "We want to help them find debt relief as quickly as possible with this new investigation program."
The new program includes efforts to fully understand the individual's financial situation, which will help tax professionals like Sacramento tax lawyer work with the IRS on a California taxpayer's behalf.
CTR's new debt relief program is an overhaul of the old system that takes into account input from taxpayers and tax specialists. The head of this project sought out advice from those familiar with IRS debt relief to determine the most important areas of improvement. It was discovered that an important focus of these restructuring efforts would be on asking the right questions early to better understand the situation that brought a client into debt and their current financial state.
CTR states that knowing a taxpayer's financial past and working with them on their future is a formula for a successful relationship. Clients in Alabama that discuss bills and expenses openly with their Birmingham tax attorney will be able to better assess what they can afford to pay toward their IRS debt.
"We want to see an increased openness between tax professionals and their clients," stated a CTR spokesperson. "The better the relationship, the less likely that there will be any concealed information or errors in filing for debt relief options. This will help speed up the process and end tax debt faster."
CTR is launching this program as a sign of their commitment to providing the best debt relief service possible. There are several options available to taxpayers needing assistance with IRS debt. Tax professionals like Oakland tax attorney are prepared under the new program to determine the best course of action for California taxpayers dependent on the amount of debt and their current financial standing.
CTR offers tax debt resolution and tax services for individuals and businesses across the United States. The company uses a three step program to create personalized strategies to help taxpayers settle their IRS debt. The company offers many services, including: state and federal tax debt resolution, IRS audit defense, tax preparation and bookkeeping.


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I'm sure that you have heard of Pareto's Principle. The principle states that, for many events, roughly 80% of the effects come from 20% of the causes. In business, the general rule is that 80% of your sales come for 20% of your clients.
When it comes to the things on your agenda, 20% percent of tasks are critical, and 80% percent are trivial. One of the things that I have people do when they work with me is to look at what's on their agenda so that we can identify which tasks on their list are critical and focus on those, and of course get rid of the trivial stuff.
Simply put, you waste your time on useless activities! Things that aren't getting you anywhere; things that are keeping you from being productive, which is why you're reading to this, right? Everybody has their major time suck…interruptions, email, social media. Maybe you know what your time suck is, maybe not, but whatever it is, it's zapping your level of productivity. If you don't know, it's time to find out and do something about it.
The rule applies in another way too…80% of your results come from 20% of your efforts. You may not be getting the results that you want, but what you're getting is a direct result of where you're putting your energy. You have to ask yourself, what do you need to be doing? What is it that needs to get done but you don't seem to have enough time for? It's time to focus your efforts on the results that you want. If you've got a major project that needs to be finished, think about the efforts you need to put forth. Let's say you want to roll out a new program for your clients, what efforts do you need to engage in? It definitely can't be the usual time suck, like the interruptions, email, social media, or whatever it might be for you. You have to shift your energy from what's killing your time to the things that are going to generate results. If that program is going to get launched, if you're going to get people enrolled in it, or get people to purchase it, what do you have to focus your efforts on? That can be a lot of work, and if it's to be done timely, you have to focus and give your time to it to make it happen. Your efforts have to go toward the results that you want with regard to that program.
Look at the outcomes you want to see if you're doing the right things and if not, where your time has to go instead. Depending on the things you do or what you give your time to some elements contribute to the outcomes you want and others contribute to the outcomes that you don't want. Be sure that you're allocating time doing more beneficial things.
If you're not getting the results that you want, it's time for an assessment of where your time is going. Then make that shift so that you start getting the results that you're looking for.


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Here is the conference call courtesy of SNY.
In a nutshell, Fred Wilpon has indicated that the Mets are looking to sell about 20-25% of the team.
Considering that the team is worth a staggering number that could come close to one billion dollars, we are talking about a potential investor who can come in with about $200 – $250 million dollars to buy themselves a minority stake in the New York Mets. That's a lot of Shake Shack burgers!
MetsBlog has some of the other highlights from the conference call:
- Exploring our options – such as the possibility of bringing in a strategic partner, or partners – is the right thing to do.
- They are 'exploring options,' and wanted to be up front about it.
- Sterling will continue to be the principle owners of the Mets.
- This is not a 'Mets issue primarily,' this is a Sterling Equities issue.
- Sterling is in confidential settlement talks with the Maddoff trustee, and he hopes they come to fruition, but, as of now, 'it is an uncertainty.'
- 'We have discussed a minority interest in the 20, 25 percent range.'
- MLB has not put any pressure on the team to seek a potential partner.
- There is no circumstance under which he would consider selling the team.
The most important thing to note here is that they will not consider selling the team.


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Vital Football T-Shirts On Sale Now
Take a look at what we at Vital Manchester City are offering in the way of T-shirts.
Our exclusive VMC shirt is selling well with others in the pipeline. If you want to order yours, click here and we'll show you how. Don't forget, every shirt sold we donate 50p towards Carl Tilson's aka mcfcthriller's charity.
To visit our Vital Network shop, click here.


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For the most part, 500.com focuses on sports lotteries – such as for the NBA, MLS and Premier League -- and has about 29% of marketshare in China. There are about 18.4 million registered user accounts.
Today I had a chance to interview the CFO, Zhengming Pan. Here's what he had to say about the IPO:
Tom Taulli: So how was the IPO process?
Zhengming Pan: It was fairly smooth. Several months ago, we had a test roadshow, which was very helpful.
Taulli: What's the business model?
Pan: It's straightforward. We operate a highly efficient online platform that sells government lottery products. For this, we get paid for services from the government.
We are 100% online so we do not carry any inventory or have logistics problems. We also bear no principal risk.
We are also one of only two entities that is authorized by the Ministry of Finance. And the other entity is not an operator in our market.
Taulli: What about engagement?
Pan: Our users are highly active on our website. On average, each active account has played 50 transactions in the first nine months of this year. That's a lot. Users must be pretty happy about our services.
Taulli: And the mobile strategy?
Pan: We established a mobile department in April 2011. Ever since then, we've seen rapid growth of mobile volume. For the first nine months of this year, 14% of overall purchase amounts came from mobile phones. We are also seeing this number going up rapidly.
Taulli: What about the startup environment in China?
Pan: I think China is a great place to start a new business. Of course, some have had success mimicking businesses in the US.
But there is also lots of innovation in China. Our company is an example. We are definitely unique in how we operate in the lottery market.
Tom Taulli (@ttaulli) operates MasterCFO, which provides outsourced CFO services for financings and mergers & acquisitions (M&A).


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6900 W 117th Ave - 3,227 and 8,750 SF, Broomfield, CO 80020Request info Add to list
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6900 W 117th Ave - 3,227 and 8,750 SF is an industrial property for lease in Broomfield, CO.
Request Property Information
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- Lance Somerville, agent and Rofo Pro


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Discussion in 'SOXual Healing' started by Cheeses, Oct 25, 2016.
Actually, tomorrow early morning.
That's what I was doing
I was at some douchey bar on North Halsted with Paco and a friend and drove home around the 11th inning convinced the SOX were going to lose because it was obvious that Marte was going to have to anchor the pitching that night.
Boy was I wrong!
Was out at our OFFICIAL 2005 playoff run haunt and stayed until the very end (worked for the family biz at the time, so hangovers weren't a huge problem). Was one of the few left standing then realized in my drunken haze that our Beloved were 1 win away.
Once Konerko hit the GS I was kind of just in awe of the inevitableness. I actually laughed when he hit that. Also when Blum hit the dong. This shit doesn't happen on the SOUTH SIDE ya know
And also they were down 4-0 in that game so it was like alright you knew they weren't gonna sweep em. Immediate 5 spot next inning.
I was in synagogue for Simchat Torah stuck listening on the radio. Think we got home just before they exploded in the 5th, IIRC.
Geoff Fucking Blum hitting the game winning HR in the World Series. That's how you know that shit was a fairy tale.
I lost my buzz around the 11th inning and had to stop drinking less I'd fall asleep.
Simchat Torah? Are you guys playing Sims, jew version?


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WELLINGTON - A couple who went shopping after lightning damaged a television left the store with a winning Lotto ticket worth $21-million.
Rosemarie and Richard Cheverie, both 64, claimed the prize in Tallahassee on Friday. They opted for the single payment of $11,283,087.31, instead of 30 yearly installments of about $700,000.
The Cheveries, who have been married 46 years, said they plan to help their children, paying off their mortgages and buying one son a car, lottery officials said.
Theirs was the only ticket to match all six Florida Lotto numbers drawn late Wednesday: 13-19-28-38-40-41.


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NASHVILLE, Tenn. – Ryan Johansen had a goal and two assists to lead the Nashville Predators to a 5-2 victory over the Kings on Monday night.
James Neal added a goal and an assist for the Predators, winners in three of their past four. Mike Fisher, Filip Forsberg and Roman Josi also scored.
Kings defensemen Drew Doughty and Alec Martinez each contributed a goal, but the Kings had their four-game winning streak snapped. Anze Kopitar had two assists.
Neal scored the game's first goal at 7:37 of the second period.
In the left circle inside the Kings' zone, Johansen won a faceoff back to Neal at the top of the circle, where he immediately fired a shot low to the far side that just slid past the outstretched left leg of Kings goalie Jonathan Quick.
Fisher doubled the Nashville lead at 13:17 of the second.
With the Predators on a power play after the Kings were called for too many men on the ice, Fisher's initial shot from the slot was denied by Quick. But the rebound fell in the crease, where a diving Fisher poked it into the net for his 12th goal of the season.
Before the opening faceoff, Fisher was honored for reaching 1,000 games played on March 3. Fisher's parents, wife Carrie Underwood, and son Isaiah were part of the on-ice ceremony.
Neal had the primary assist on Fisher's goal, giving him his 400th NHL point.
Forsberg tapped home the rebound of Craig Smith's shot at 15:23 of the second period to make it 3-0.
The goal was the 30th of the season for Forsberg. He became the fifth player in Predators history to score 30 or more in a season, joining Jason Arnott, Paul Kariya, Steve Sullivan and Patric Hornqvist.
Quick was lifted in favor of Jhonas Enroth, who began the third period in net for the Kings. Quick stopped 24 of the 27 shots he faced through 40 minutes.
The Kings scored 32 seconds into the third when Doughty's shot from the blue line beat goalie Pekka Rinne, who finished with 28 saves.
Martinez scored at the 8-minute mark with a wrist shot from the left point through traffic, but Nashville regained its two-goal advantage at 11:45 when Johansen redirected Shea Weber's shot from the right side.
Josi added an empty-net goal in the final minute.


text: 017 FGUS74 KOHX 201300 ESFOHX TNZ005>011-023>034-056>066-075-077>080-093>095-210100- Hydrologic Outlook National Weather Service Nashville TN 700 AM CST Tue Feb 20 2018 ...Potential Flooding Mid to Late Week Due to Several Rounds of Moderate to Heavy Rainfall... A slow moving frontal system is forecast to push into Middle Tennessee on Wednesday and linger across the area into Thursday. This system will provide some parts of Middle Tennessee with moderate to heavy rainfall. A second system will move in over the weekend and bring another round of moderate to heavy rainfall to the same areas. Right now, it looks like northwestern Middle Tennessee will receive the most rainfall over the next five days, with rain totals between Wednesday morning and Sunday morning expected to average between three to five inches, with locally higher amounts above 6 inches possible. Most of the heaviest rainfall is forecast north and west of a line from Lobelville to Portland, including areas around Clarksville, Springfield and Dickson. Other parts of the Mid State are forecast to receive between one and a half and three inches over the next five days. The periods of heaviest rainfall are expected to be Wednesday afternoon and Wednesday night, and again Saturday afternoon and Saturday night. Since the grounds in Middle Tennessee are still very wet from previous rains, any additional heavy rainfall could lead to localized flash flooding as well as river and stream flooding. Stay tuned to the latest forecast and listen for any watches or warnings that might be prompted by the heavy rainfall over the next five days. $$ 19


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This submarine paper model is the Captain Nemo's Nautilus, based on the novel "Twenty Thousand Leagues Under the Sea", the papercraft is designed by ZRP Art Craft. The Nautilus is the fictional submarine featured in Jules Verne's novels Twenty Thousand Leagues Under the Sea (1870) and The Mysterious Island (1874) / (2012). Verne named the Nautilus after Robert Fulton's real-life submarine Nautilus (1800). Three years before writing his novel, Jules Verne also studied a model of the newly developed French Navy submarine Plongeur at the 1867 Exposition Universelle, which inspired him for his definition of the Nautilus.
Captain Nemo, also known as Prince Dakkar, is a fictional character featured in Jules Verne's novels Twenty Thousand Leagues Under the Sea (1870) and The Mysterious Island (1874).
Nemo, one of the most famous antiheroes in fiction, is a mysterious figure. The son of an Indian Raja, he is a scientific genius who roams the depths of the sea in his submarine, the Nautilus, which was built on a deserted island. Nemo tries to project a stern, controlled confidence, but he is driven by a thirst for vengeance and a hatred of imperialism and wracked by remorse over the deaths of his crew members and even by the deaths of enemy sailors.
Twenty Thousand Leagues Under the Sea is a classic science fiction novel by French writer Jules Verne published in 1870. It tells the story of Captain Nemo and his submarine Nautilus as seen from the perspective of Professor Pierre Aronnax. The original edition had no illustrations; the first illustrated edition was published by Hetzel with illustrations by Alphonse de Neuville and Édouard Riou.
You can download this paper craft model from here: Captain Nemo's Nautilus Submarine Paper Model Free Download


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Ray LaMontagne
Raymond "Ray" Charles Jack LaMontagne (pronounced /lɑːmɒnˈteɪn/) (born June 18, 1973) is a Grammy-award winning American singer-songwriter. He has released four studio albums, Trouble, Till the Sun Turns Black, Gossip in the Grain and God Willin' and the Creek Don't Rise. In the UK, Trouble was a top 5 hit, and the title track of the album was a top 25 hit. Till the Sun Turns Black was a top 40 hit in the U.S. A soft-spoken person who is known for his raspy voice, LaMontagne has won a number of awards for his music and has performed at several charity events.
Sursă: Wikipedia engleză
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Traveling Luck for Northbelt Business Park, Georgia, United States
Where is Northbelt Business Park?What's around Northbelt Business Park?
Wikipedia near Northbelt Business Park
Where to stay near Northbelt Business Park
The timezone in Northbelt Business Park is America/Iqaluit
Sunrise at 08:18 and Sunset at 19:23. It's light
Latitude. 33.9269°, Longitude. -84.2161°
Weather near Northbelt Business Park; Report from Atlanta, De Kalb-Peachtree Airport, GA 11.6km away
Weather :
Temperature: 20°C / 68°F
Wind: 0km/h
Cloud: Few at 11000ft
Weather :
Temperature: 20°C / 68°F
Wind: 0km/h
Cloud: Few at 11000ft
Satellite map around Northbelt Business Park
Loading map of Northbelt Business Park and it's surroudings
Geographic features & Photographs around Northbelt Business Park, in Georgia, United States
- Local Feature;
- A Nearby feature worthy of being marked on a map..
- Northbelt Business Park (0km)
- Norcross Plaza Shopping Center (0.9km)
- Norcross Shopping Center (1km)
- Norcross Tucker Industrial Park (1.8km)
- Southern Plaza Shopping Center (2km)
- Oakbrook 85 Business Park (2.5km)
- Norcross I-85 Center (2.7km)
- Oakbrook Center (2.8km)
- Oakbrook Square Shopping Center (2.9km)
- Oakbrook Office Park (3km)
- Weaver Way Industrial Park (3.1km)
- Winter Village Shopping Center (3.5km)
- First Baptist Church of Norcross (3.5km)
- Cedar Village Shopping Center (3.8km)
- Kingston Industrial Park (3.8km)
- North Rockbridge Plaza Shopping Center (3.8km)
- Market Place Shopping Center (3.8km)
- Pleasantdale Business Park (3.9km)
- Peachtree Crossing Business Park (4km)
- Peachtree Corners Shopping Center (4.3km)
- Indian Trail Industrial Park (4.3km)
- Indian Village Shopping Center (4.4km)
- Oakbrook Business Park (4.4km)
- Peachtree Corporate Center (4.4km)
- Exit 37 (4.6km)
- populated place;
- a city, town, village, or other agglomeration of buildings where people live and work.
- Sheffield Forest (0.8km)
- Springdale Estates (1.7km)
- Norcross (2km)
- Oakbrook Station (2.9km)
- Mechanicsville (3.1km)
- Danbury Village (3.5km)
- Knollwood (3.7km)
- Stonewood (4km)
- Edgemoor (4km)
- Overlook (4.1km)
- Hyde Park (4.3km)
- Jones Mobile Home Park (4.5km)
- Glen Leaf (4.6km)
- church;
- a building for public Christian worship.
- Galilean Church (2.4km)
- First United Church (3.1km)
- Saint James Church (3.5km)
- John Wesley United Church (3.6km)
- Glover Church (4km)
- Saint Patricks Church (4.2km)
- Pilgrimage Church (4.4km)
- school;
- building(s) where instruction in one or more branches of knowledge takes place.
- Summerous Middle School (2.2km)
- Norcross High School (2.6km)
- Norcross Elementary School (2.8km)
- Mechanicsville School (historical) (3.7km)
- Rockbridge Elementary School (4.5km)
- airport;
- a place where aircraft regularly land and take off, with runways, navigational aids, and major facilities for the commercial handling of passengers and cargo.
- Jimmy Carter Boulevard Precinct Heliport (2.2km)
- Site 3PC Heliport (3.5km)
Accommodation around Northbelt Business Park
- building(s);
- a structure built for permanent use, as a house, factory, etc..
- Norcross Public Library (1.8km)
- Norcross City Hall (2.4km)
- park;
- an area, often of forested land, maintained as a place of beauty, or for recreation.
- Best Friends Park (1km)
- Norcross Historic District (2.1km)
- post office;
- a public building in which mail is received, sorted and distributed.
- Norcross Post Office (2.3km)
- reservoir(s);
- an artificial pond or lake.
- Lake Windsor (4.4km)
- dam;
- a barrier constructed across a stream to impound water.
- Lake Windsor Dam (4.4km)
- section of populated place;
- a neighborhood or part of a larger town or city.
- Peachtree North (subdivision) (3.3km)
- cemetery;
- a burial place or ground.
- Greer-Bowen Cemetery (4km)
Wikipedia entries close to
Northbelt Business Park
- Norcross, Georgia city city o
- Doraville, Georgia city city o
- Peachtree Corners, Georgia city city o
- Berkeley Lake, Georgia city city o
- Lilburn, Georgia city city o
- Tucker, Georgia city city o
- Chamblee, Georgia city city o
- Dunwoody, Georgia city city o
- DeKalb-Peachtree Airport airport airport
- Luxomni, Georgia city city o
- Duluth (Georgia) city city i
- Mountain Park, Gwinnett County, Georgia city city o
- North Atlanta, Georgia city city o
- Clarkston, Georgia city city o
- Stone Mountain, Georgia city city o
- Sandy Springs, Georgia city city o
Airports close to Northbelt Business Park
Dobbins arb(MGE), Marietta, Usa (35.4km)
The william b hartsfield atlanta international(ATL), Atlanta, Usa (47.6km)
Middle georgia rgnl(MCN), Macon, Usa (188.1km)
Lovell fld(CHA), Chattanooga, Usa (193.8km)
Anderson rgnl(AND), Andersen, Usa (193.9km)


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Lost love story defied canned heat
Page Tools
Gloria Swanson feared that Beyond the Rocks had gone forever.
Photo: Supplied
A silent movie starring Gloria Swanson and Rudolph Valentino has made it back to the screen after being given up as lost, writes Gregory Crouch.
Even perfectly scripted Hollywood love stories can, literally, turn toxic with time.
What happened leading up to a showing of the long-lost silent film Beyond the Rocks, starring Gloria Swanson and Rudolph Valentino, possibly the silver screen's first male sex symbol, is a case in point.
Film fanatics, historians and Swanson herself had feared the only motion picture to feature two of the biggest stars of the 1920s - a romantic melodrama in which love triumphs over money - was gone forever.
"Does anyone know of a print anywhere of Beyond the Rocks, the film Rudy Valentino made with me in 1921?" Swanson asked in her 1980 autobiography, Swanson on Swanson, lamenting its disappearance. "That, after all, was supposed to be the great virtue of pictures - that they would last forever."
Just another Hollywood illusion, as it turns out.
Silent movies such as Beyond the Rocks were recorded on flammable nitrate film that can dissolve and emit toxic gases. Some studies indicate that about 80 per cent of all silent films made in the United States have either been destroyed or have disintegrated into potential health hazards.
That is why archivists at the Filmmuseum in Amsterdam donned gasmasks and protective garments before sifting through an eccentric Dutch collector's donation of 2000 or so rusty film canisters, which ultimately yielded Beyond the Rocks, a 1922 release.
"Since we were the first ones to open these cans, we didn't know what was really in there," says Elif Rongen-Kaynakci, a Filmmuseum archivist. "That's why we had to wear gasmasks."
Decked out in their protective gear, she and her colleagues watched in delight as snippets of pent-up glamour flickered before them: There was Swanson in lavish beaded gowns and a fortune in jewellery; and Valentino, again oozing his legendary sex appeal as the other man in a classic love triangle.
"He is very charming, nobody can deny this," Rongen-Kaynakci said with a giggle. "Seeing him move in the film, he's charming, he's sweet, he's just great."
Over a three-year period, the Filmmuseum's archivists waded through a virtual hazardous waste site of decaying film before they discovered all seven reels of Beyond the Rocks. Researchers say they believe that the 80-minute film they have reconstructed is nearly complete, based on comparisons with the script, with only about seven minutes missing. The Filmmuseum restored its sepia tint, stabilised some images, repaired scratches and tears as best it could.
Swanson plays Theodora Fitzgerald, a young woman who marries a rich, older man for the sake of her family, which has fallen on hard times. However, fate (which is to say the screenwriter Elinor Glyn) throws her into the arms of Valentino as the English aristocrat Lord Hector Bracondale.
He plucks her from two perilous situations - an overturned boat and a tumble down a mountainside - before pursuing her to the ends of the earth, which in those days were filmed in the Rocky Mountains and greater Los Angeles area.
Milestone Film and Video Company, a New Jersey distributor, hopes to re-release Beyond the Rocks at film festivals and in theatres around the country.
The film was not a huge success at its premiere in 1922. The New York Times panned it as a tale of two clotheshorses. But its discovery is considered significant today because so many silent films have vanished.
"Outright destruction, neglect and basic deterioration" is to blame, laments Patrick Loughney, curator of motion pictures at the George Eastman House in Rochester, one of the largest film archives in the US.
In the 1920s, many studios considered their movies to be mere products that could be discarded once they had earned money.
Loughney applauds the discovery of Beyond the Rocks because it gives "context to one of the least understood eras of American film history".
As director Martin Scorsese says in a videotaped introduction to the showing, it was one of the first movies to feature two leading stars. "That alone makes the discovery of Beyond the Rocks a noteworthy event," he says.
The film's unlikely saviour was the collector Joop van Liempd, himself something of a legendary figure in his home city of Haarlem.
Van Liempd was a loner who has remained as much of a mystery to curators as many of the crumbling films he collected. By the time of his death in 2000 at the age of 87, he had amassed an enormous collection of film canisters that, fearing for their safety, he spread out over at least five locations, friends said.
But few of the canisters were labelled and many, archivists say, had never been opened. Some - surprisingly given their flammability - were stored next to a gas heater, museum officials said.
Jan van den Brink, a Filmmuseum curator, suspects that van Liempd might never have seen Beyond the Rocks, at least not the copy in his collection.
"With 2000 cans of film, you can't see them all," van den Brink says. "It would have taken a lifetime."
- New York Times
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It has been a little while since I've posted, but in this case it is a good thing.
Over the past few months I have been settling into my new position as a Network Engineer and working on many projects in those regards that have taken much of my time.
Outside of these normal work projects, I have taken up a new hobby: streaming.
A few weeks ago I was speaking with a friend about playing video games and we decided it would be fun to try streaming a video game so that other people could see what fools we were when we played games together. After our first session, I decided to keep the stream going to see what I could do with it.
Up to this point I had not even watched very many streams, so I didn't really know what to expect. I started doing some research on what it would take to get started in streaming. I found it didn't take very much, so off I went.
Currently I'm streaming whatever game I am playing at the time (mostly League of Legends and Minecraft) and I've already built up a decent community with other streamers.
I'm hoping that I can continue streaming for the foreseeable future and possibly start monetizing it for some additional income, just like I do with IT Support right now.
Feel free to check out the stream at LintyJuggler90 Twitch Channel.