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NAKIVO is Named the Fastest-Growing Company in 2013 by StorageNewsletter
NAKIVO's explosive growth rate in VMware data protection market is acknowledged by StorageNewsletter - a leading IT media company.
Campbell, CA, United States — February 19, 2014
NAKIVO Inc., the leading VM data protection software provider for VMware virtualized environments, has announced today that StorageNewsletter - a market-leading IT news and media company – has named NAKIVO #1 among the top growing storage startups and private companies in 2013.
Being profitable since founding in 2012, NAKIVO has shown exceptional revenue results throughout 2013 and reported a revenue growth of 800%. This has put NAKIVO on top of the list of the fastest-growing companies in 2013, outpacing all of its competitors.
Such a high growth rate is attributed to three factors: a market need for an intuitive, fast and, affordable ESX(i) VM data protection, a feature-rich solution - NAKIVO Backup & Replication - that meets the market demand, and the large number of NAKIVO partners who delivered the solution to the market.
Aiming to be 100% channel-based, NAKIVO has signed up 8 software distributors in 2013 and increased its Partner Program to over 380 resellers in 55 countries across the Americas, Europe, Africa, Middle-East, and Asia. Additionally, NAKIVO has introduced Cloud Provider Program with per-VM Licensing, enabling Managed Services, Cloud, and Hosting Providers to introduce Backup-as-a-Service (BaaS), Replication-as-a-Service (RaaS), and Disaster Recovery-as-a-Service (DRaaS) to their customers. In just four months, NAKIVO has added 20 cloud Service Providers to its program.
With the help of its partners, NAKIVO has grown its customer base to over 1,500 customers across the Americas, Europe, Africa, Middle-East, and Asia. Moreover, NAKIVO has not only won multiple deals against competition, but also gained more than 10% of its new customers by switching existing competitor customers to NAKIVO.
"We are pleased with the results of the StorageNewsletter market study and their recognition of NAKIVO as being the fastest growing company in the storage and data protection industry," said Bruce Talley, CEO and co-founder of NAKIVO. "Our customer base and revenue growth continues to accelerate quarter over quarter. In 2014, we plan to introduce more market-disruptive features that along with the help of our outstanding channel partners will enable us to gain additional market share and strong growth."
- Overview: /vmware-backup/
- Datasheet: nakivo-vm-backup-datasheet.pdf
- Cloud Backup: /partner/cloud-provider-program/backup-as-a-service/
- Success Stories: /customers/success-stories/
- Trial Download: /resources/download/trial-download/
Headquartered in Silicon Valley, NAKIVO is a privately-held, self-funded software company that has been profitable since founding in 2012. With more than 1500 customers worldwide, NAKIVO develops and markets a line of next generation data protection products for VMware vShpere ESXi virtualized environments with live applications & databases, including Microsoft Active Directory, Microsoft Exchange, Microsoft SQL, and Oracle. NAKIVO provides one of the most affordable, highly reliable and easiest to use VM backup and replication solution for enabling SMBs to protect, encrypt, compress and deduplicate VM data onsite, offsite and in the cloud. NAKIVO also enables hosting, managed, and cloud services providers to create and offer VM Backup-as-a service, Replication-as-a service and DR-as-a service, to their customers. NAKIVO was the first virtualization backup specialist to offer VM backup to public clouds such as Amazon to SMB customers and multi-tenancy to service providers and enterprise customers. For more information, please visit www.nakivo.com.
Follow us on Twitter: @NAKIVO
Connect on Facebook: www.facebook.com/NakivoInc
Join us on LinkedIn: www.linkedin.com/company/nakivo
Contact us: Yana Petrenko, MarCom Manager / email@example.com / +1 408 916 5955


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Zootopia & Dolby Cinema at AMC Prime
By Erin
*This is a sponsored post, all thoughts and opinions remain my own.*
Disney's Zootopia opens March 4th in theaters everywhere! I can tell you without a doubt that this is a film you will not want to miss. Jam packed with excitement, lovable characters, quick wit, some catchy new tunes, and a heart-warming message to warm your soul. You will love everything about this film! What's even more to love is that to celebrate this special occasion, I have an exciting giveaway of (2)-4-Packs of tickets to watch the film in Dolby Cinema™ at AMC Prime™AMC Century City 15 theatre in Los Angeles, California, on Saturday, March 5th, 2016 at approximately 11:00 a.m. Read below to learn more about this fun action-adventure film that any family will love and how to enter for a chance to win tickets today!
Seeing Disney's Zootopia in Dolby Cinema at AMC Prime combines the most powerful image and sound technologies with spacious, reserved recliners and I am so excited to see the movie this way!
See spectacular imaging and vibrant colors from all the animals that make up Zootopia. Hear sound that moves all around you. And feel true comfort in recliners that pulsate with the action.
Dolby Cinema delivers the total cinema experience. It combines powerful image and sound technologies with inspired cinema design to make every visit a completely captivating cinematic event.
Dolby Cinema will include Dolby Vision—a state-of-the-art authoring and playback imaging technology that enables the cinema to bring the story alive onscreen. Outfitted with Dolby Atmos, a Dolby Cinema transports audiences into the movie with breathtakingly real sound that fills the cinema and flows all around them.
When these technologies are combined with the sophisticated Dolby Cinema design, everything comes together to pull moviegoers more deeply into the story and transform their visit into an event. For more information, please visit the Dolby Cinema web page.
Here is a list of all the theaters that currently have Dolby Cinema at AMC Prime. The tickets for this giveaway are ONLY for the AMC Century City 15, although I hope you live near one of these theaters to be able to enjoy the movie with Dolby Cinema at AMC Prime!
New Jersey
Enter for a chance to WIN Tickets in Dolby Cinema at AMC Prime in Century City!
Now YOU can enter for a chance to WIN tickets for this amazing experience to go see Disney's Zootopia in Dolby Cinema™ at AMC Prime™ at AMC Century City 15 in California, on Saturday, March 5th, 2016 at approximately 11:00 a.m. You'll see the movie in the most spectacular way with outstanding imaging and vibrant colors, and hear sound that moves all around you. Feel true comfort in recliners that pulsate with the action. Enter by clicking on the Rafflecopter link below and choose which ways you'd like to enter. 🙂
***If you win, you will need to be available to pick-up the tickets at the will-call of the AMC Prime™ in Century City, California, the day of the event. Tickets are ONLY for the AMC Century City 15 location for the 11:15am showing***
Good-Luck!! 🙂

subset: commoncrawl


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'At Work'
Studio and production as a subject of today's art
Museum für Gegenwartskunst Siegen, November 17, 2013 − March 09, 2014
This exhibition is conceived as an essay and deals with the way in which consideration of the making and places of art is reflected in the artworks themselves. In the history of art, the studio was the traditional place in which artworks were produced, subsequently to be released into the world as transportable objects: into galleries, museums and private collections. At the same time this 'order' of places and their functions corresponded to the unity of author and artwork, a stylistic and genre-specific identity. There are no longer any fixed orders and attributions in today's art production. Concept Art has no uniform style; artisan production is delegated, and playful and associative strategies now create manifold constellations of references. The function of the studio has become more relative in this context. Interestingly, in the current 'crisis' of the studio the art-historical genre of the studio picture is entering into many artists' horizons, once again becoming a symbolic "motif", often with a melancholy or ironic undertone. Meanwhile, there is reflection on the production of art in other places. This exhibition is conceived as an essay and deals with the way in which consideration of the making and places of art is reflected in the artworks themselves. 20 international artists will be showing works or working contexts from the last 15 years as well as works newly produced on the occasion of the exhibition, which focus on questions of authorship, the finding of ideas, style, participation, and the relations of the studio and public exhibition locations. It is the 'shadows' of art – based on a formulation by Roland Barthes –, which are finding their way here into the artwork itself.
Pawel Althamer, Tacita Dean, Katharina Grosse, Bruno Jakob. Christian Jankowski, Yuri Leiderman, Rita McBride, Mai-Thu Perret, Hermann Pitz, Sarah Rossiter, Giorgio Sadotti, Karin Sander, Adrian Schiess, Paul Sietsema, Deb Sokolow, Kathrin Sonntag, Annika Stroem, Elizabeth Wright, Andrea Zittel, and Artur Zmijewski
Curators: Eva Schmidt and Roman Kurzmeyer
Exhibition catalogue, edited by Roman Kurzmeyer, Eva Schmidt
Designed by Studio Manuel Raeder (Santiago da Silva and Manuel Raeder)
Texts (German/English) by Roman Kurzmeyer, Eva Schmidt and artists
256 p with 125 coloured illustrations, 275 x 205 mm, softcover with embossing, ISBN 978-3-86442-070-2
Available at Snoeck


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After footage leaked of an 18-year-old Tyga acting very much like a "busta" on the un-aired MTV show, "Bustas," people began clowning him badly for his embarrassing actions in front of the camera. On the show, he went so far as to state that he didn't have a hard life growing up, that he lives in the Valley and that his parents "have a Range Rover". He also revealed how he got his name, which didn't have a hard story behind it, but rather his mother used to call him Tyga because he "looked like Tiger Woods".
Back in those days, he probably didn't envision his career would blow up as much as it has, nor did he think of the possible damage this footage would do to the overall level of respect he gets in the game. He's now come forward, according to TMZ, and claimed that he lied on the TV show to make himself sound like he had a better life than really was the case. Tyga is now saying that he really did grow up in Compton, CA and that the time he lived in the Valley was only for a very short period of his life.
Tyga isn't worried about the video damaging his career because "he still has plenty of money and will still have huge hits." YMCMB is also standing behind him during this comical time.
Source: globalgrind.com


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Although the Celtics European trip will last another couple days, they wrapped up their overseas exhibition schedule today with a 105-75 win in Milan, Italy.
As is always the case, there were some useful nuggets to be taken out of this preseason affair, so let's take a look at some of the main storylines and what they mean (if anything) moving forward.
Rajon Rondo was perfect early: No really, he was. 17 points in the first quarter on 7-of-7 shooting. These weren't all layups either as the All-Star point guard was pulling up confidently early and often with deep jumpers from beyond the elbows to punish a Armani squad that was going under his picks. Plenty of aggressiveness early in these two contests on the offensive end, which is the kind of aggressiveness Doc Rivers would love to see moving forward as Rondo makes use of his improved supporting cast.
Jared Sullinger and Jason Terry started: Mere experimentation by Doc or something more? With just two preseason games under their built, it's far too early to tell. However, it's easy to see the logic behind shifting either guy in the starting five. We've yet to see Sullinger face off with legitimate NBA competition but he can perform a role that the C's haven't had in years up front amongst the starting five (an active offensive rebounder who can finish).
The former Ohio State star finished nine points and seven rebounds (three offensive) this afternoon. While Sullinger's size may be a flaw, Garnett's presence on the floor can help hide his defensive limitations. Again, it's early and I certainly don't expect to see Sullinger work his way into the starting five anytime soon, but it's fun to see Doc tinkering with an idea that could have quite some good karma with it.
As for Terry, he's the guy that will more often than not be sharing the floor with the starters in the final five minutes of most close games. Since the starters aren't playing extended minutes now, it made since for him to get some run with Rondo, Pierce and company to continuing improving that chemistry and he did not disappoint with 11 points, three assists and a couple heads up steals for good measure.
Darko continues to contribute: Chris Wilcox's back injury has given the former second overall pick an opening for big-time minutes early in the preseason here and Darko continues to pleasantly surprise the Boston faithful with his rebounding and passing acumen. He's always been a strong shot blocker for the majority of his NBA career so that's no surprise, but his contributions in other parts of the box score (9 rebounds, 3 assists) in addition to the blocks (4) make his regular insertion into the rotation right out of the preseason a distinct possibility. The next step though is continuing the progress against stiffer competition this week back in the States.
A bench that can score: Brandon Bass, Courtney Lee, and Jeff Green combine for 39 points on 14-of-19 shooting, all while creating plenty of these scoring chances for themselves in the half court? An unfamiliar and welcome sight for this squad.
The Celtics return back to Boston on Wednesday from Italy, and have a full five days off before their next preseason tilt in Hartford against the Knicks on Saturday night.
Brian Robb
Latest posts by Brian Robb (see all)
- Wizards Crush Celtics in Game 4, Tie Series at 2 - May 8, 2017
- Who Should the Celtics Start vs. Wizards? - May 6, 2017
- Celtics' Biggest Challenge Down the Stretch Will Be Overcoming Their Inconsistency - March 24, 2017


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"I was diagnosed with GSB back in early June 2011 - I had (have?) it orally and I'm concerned that I also have it vaginally. I had an irregular pap in late July, and it turned out that I also have HPV. So my immune system is obviously..."
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Moving!!! Again!
We are back where we started 20 years ago! Near the corner of Eubank and Candelaria at 9809 Candelaria Blvd Bldg C. See you there!
Methylation Basics
Methylation Basics
December 27, 2015
So, you've heard the word methylation but are unsure exactly what that means? It is definitely the hottest topic in health care today. Not just in the natural health care world but also in mainstream medicine. Information that comes from having your genetic test done is providing incredible information about who we are and how we think, how we respond to the environment, how we detox, how we repair our bodies and our susceptibility to diseases. Drugs companies are making use of our genetic data to determine appropriate drugs for patients based on their genetic ability to process those drugs.
The ability to form methyl groups and use those methyl groups in the body is an incredibly interesting and somewhat complicated topic. Genetic defects in the methylation pathway are the underlying cause of a tremendous number of health issues today. Overlooking the importance of methylation can leave you spinning in trying to get to the root of chronic health issues.
As a natural health care practitioner of 27 years, I can speak to this topic. I spent years thinking I was getting to the root of health issues when I was really just treating the symptoms. Since I have begun analyzing genetic data from such sources as 23andme, and Doctors Data, I have come to realize that there was a whole layer of disease cause that I had not even considered.
Thanks to pioneers such as Dr. Amy Yasko and Richard Van Konynenberg, we have begun to go much deeper in our quest for answers in health care. Combining this knowledge of how the methylation pathway works along with Wholistic Kinesiology has allowed us to explore uncharted areas of health care and forge new pathways in our search for answers.
We will be taking a journey through the methylation pathway in an effort to find answers to some of our most confounding health care issues. It is a symphony and must be considered as a whole. Thus the name Wholistic Methylation.
This is the beginning of an incredible journey through the human genome and the origins of disease and dysfunction in the body. It is a fascinating journey that includes forays into our ancient ancestors, epigenetics, changes in dietary practices, mental health issues, the origins of our personalities, why we do the things we do and so much more.
I hope you will join me on this journey as I begin the blogging process. This is a new venue for me but I think it is an important topic. I am lecturing around the united states for a supplement company, Nutri-West but I also want to bring to the public the exciting new information as it develops in the hopes that it will change lives. I know that the results I and my colleagues are getting are tremendous and I want others to have access to this information as well.
I have seen a tremendous amount of misinformation presented in blogs, seminars, webinars etc. So I have to speak to this. One thing I want to make very clear is this: Taking supplements to balance just one of the genetic variants in the pathway is shortsighted and will not bring lasting results. I want to bring clarification to the subject so that it will be used correctly. I have seen many people dabble in this topic without sufficient understanding of the material and give up too easily. The rewards are incredible for those that forge through the material and begin the correct application of the information.
This blog is designed for doctors, patients, practitioners of all kinds to help you along the way toward optimal health. I think there are some great answers here. Stay tuned!!
Dr. J Dunn
Methylation Update
With Methylation becoming a real buzzword in the natural healing world, lots of practitioners are jumping on board. There are many opinions out there about how to handle methylation defects and how to evaluate them. What I have learned in the last two years is that it is a complicated and very individual process. One thing that is certain is that you cannot treat each genetic variant in the pathway by itself. The variants all have an effect on each other, so to say that you should take methylfolate if you have a MTHFR defect is very short sighted. I consider it a symphony where all parts of the pathway need to be working together to make the cells and the body sing!
We have been using the MTHFR Support reports to go beyond the genetic genie report. This has given us some insight to the other genetic variants that can affect the balance of hormones, enzymes and detoxification ability of our health. All of which can impact the methylation pathways. It is important to determine as many variants as possible and address all that we can together.
Using the methylation vial kit and kinesiology, we can begin to determine which areas of the pathway are being affected. If you find a substrate (such as folic acid) weak with the vials you can begin to suspect a genetic variant in the conversion of folate to methylfolate (MTHFR). If your patient tests weak to vitamin D, adding vitamin K2 and vitamin A with it and they test strong, then we suspect a variant in the Vitamin D receptor gene. (VDR) These particular variants are very detrimental to our overall health. Not being able to absorb Vitamin D as you can imagine will lead to susceptibility to cancers of all kinds, chronic infections, immune system dysfunction, not to mention osteoporosis and bone mineral density issues.
We have developed a number of aids in assisting with trying to evaluate the methylation process. Using the kits mentioned above and the methylation charts either static (available on this sight) or interactive chart (available for sale) we can really get a good idea of where the blocks are and develop supplement regimens that help the body function normally. We also have guides to the genetic variants (SNiPs) and vial tester guides available on request. (see email below).
When all variants are either bypassed or helped with cofactors, many symptoms begin to disappear rapidly. The first things we see happen are mood begins to lift, focus and concentration are better, energy is better. Over time, pain in joints lifts, digestion improves, leaky gut repairs, food and environmental allergies improve or disappear, chronic infections of all kinds resolve (I've even seen Lyme and EBV clear up!)
We are truly getting deeper than ever to the real causes of disease instead of treating symptoms or the results of genetic variants. This is a very exciting time for natural healing!
Dr. J Dunn
If you would like a consultation and analysis of your genetic reports email email@example.com (Standard hourly fee will apply).
EFT-Emotional Freedom at Last!
Emotional Freedom technique is sweeping the country! It is the most profound technique to come out of natural healing and energy medicine to date. With this technique many have been able to overcome limiting beliefs, phobias, traumatic events and anxieties very quickly and permanently. This stunning discovery in how the body works sound almost too good to be true. What has been discovered is that "The cause of all negative emotions is a disruption in the body's energy system", according to discoverer Dr. Roger Callahan.
Dr.Callahan, a psychologist with over 40 years experience, developed Thought Field Therapy. TFT was further refined by Gary Craig a Stanford Engineer who became interested in personal improvement. He developed the technique known as EFT, which is changing the practice of psychology. You no longer have to spend years in talk therapy to achieve results. You can achieve amazing results and progress with emotional issues sometimes within minutes. There is no long drawn out "tell me your life story" feature to EFT. There are no pills. There is no painfully reliving past traumatic events. By correcting the energy imbalances created by emotions you can take the charge out of them.
Our electrical systems are vital to our physical health. When energy stops flowing, we die. About 5,000 years ago, the Chinese discovered a complex system of energy circuits that run throughout the body. These energy circuits or meridians are the core of many healing techniques today. EFT uses this knowledge to unlock the trapped emotions in the body and release there hold on the conscious and unconscious mind. Once the energy meridians are balanced the emotional intensity goes away. This is the most powerful thing you can learn about your emotions, they are caused by energy disruptions. If a memory can no longer cause a disruption in the body's energy system, the negative emotion can no longer occur.
These techniques can give you the freedom that allows you to blossom. They allow you to rise above whatever hidden hurdles keep you from performing to your capacity. Freedom to earn that greater income, make those business calls, start a new business, improve your golf score, lose that weight, approach people with ease, get rid of that phobias that keeps you from living your life fully.
Can you imagine freedom from years of self doubt and torment? We see consistent results using this technique. Most problems vanish in minutes, a few problems take longer, maybe two or three brief sessions. The most difficult ones tend to require anywhere from a few days to a few weeks. However, these are in the minority.
A new innovation such as this technique naturally meets with skepticism because it violates people's beliefs. EFT contradicts just a about every belief there is about psychology. Quick relief for "difficult" emotional problems are generally considered impossible. If your reaction to the claims in the previous paragraph sounds a little too good to be true, I'm not surprised. This is a common response to these techniques. But I urge you to try it out before you pass judgment. I have seen these techniques perform true miracles right before my eyes. And people make improvement despite their skepticism.
One other advantage of this technique is that once you learn it (it's easy to learn) you can perform it on yourself. You can continue to work on your emotions at home on a continuous basis. (I do this technique on myself almost daily!) And according to Dr. Mercola, one of the biggest fans of EFT, "Perhaps the biggest limit you will encounter in using EFT is not recognizing how much you can do with it. " You'll never know how good you can be until you try!!
I introduced EFT to the professional athletes and trainers at a Charles Poliquin seminar in Phoenix several years ago. It was incredibly well received by these folks. They saw the true value of clearing underlying belief systems in their clients. Many of these clients are Olympic athletes, trying to achieve their personal best. We all know that our thoughts and beliefs are incredibly important in these endeavors. The mental game is every bit as important as our physical game in achieving the results we are after.
Wholistic Kinesiology Center is located at 7103 4th St. NW, Albuquerque, NM, opened in 1996 by Dr. J Dunn. The center has become the focal point for education in Natural healing in the community. Seminars and lectures are given on a regular basis. Dr. J Dunn is the developer of Wholistic Kinesiology®. Six month courses in Wholistic Kinesiology are taught starting in January. (EFT is taught as part of the course) All of our practitioners are trained in EFT.
Come check it out-you'll be amazed!!
For a Free mini demo Call 275-9809
The Best of Both Worlds, Natural Health and Allopathic Medicine
Dr. J Dunn
The Line has been drawn and it needs to be erased. Where is it written that you can't combine natural medicine with allopathic medicine?? It is very rare that you run into a practitioner on either side and get a balanced viewpoint about the other. With all the talk about integrative medicine, there really doesn't seem to be movement on either side to really do this. I believe it's up to us as patients or warriors in the battle of preventive health to call the shots and demand that both sides begin to bury the hatchet.
I recently had a brush with death at the hands of a medical intervention. I was infected with a rare and slow growing bacteria in the hospital when there for a routine appendectomy. I eventually had to return to the hospital to get medical intervention again, as I was unable to get on top of this infection through natural means. I was on three different antibiotics. My doctor asked if I was taking any natural supplements and asked me to stop. I refused. They were helping me avoid a yeast infection, reducing the inevitable gas and bloating that occurs with these medications and keeping my energy up. I know not to combine medications with certain supplements, so I am confident in what I was doing.
Really, I am not a self proclaimed medical basher, but I learned a few things through this experience. One very important lesson is that there is a place for both philosophies and they can be used together very effectively. So many people are frightened by their medical doctors into not combining the two because they don't have any knowledge of natural medicine, and natural health practitioners are equally as guilty of believing that all procedures and medications given by the MD's are bad, poisonous, dangerous and completely unnecessary.
It's time for a new paradigm and a blurring of the lines between the two disciplines. We need to start demanding what we want as consumers of health care. It is up to us to make choices and to vote with our feet. I propose the following bill of rights for us medical consumers that want more out of our health care professionals.
Patient Bill of Rights
We demand to be listened to, we demand that you take your time and really understand our complaints and feel our pain.
We reserve the right to choose our own course of medical care with your expert advice to help guide us and we don't want to be bullied into procedures or medications that we don't fully understand.
We demand to have every procedure or medication or supplement explained to us in a manner and in language that we can understand. We need to know the risks, outcome and goals of each.
We want to be given choices, where a noninvasive, less expensive procedure will suffice let us be given the choice.
We demand health care providers that will refer us out to others more competent when they are not the right one to take care of our needs. We want practitioners that put our health above their egos.
Using Natural Health Care with modern Allopathic Medicine: What would that look like?
How about a combination of the two that really covered all our needs. What if we could go to a natural health care practitioner that took care of our needs for preventative medicine. They would check us for nutritional deficiencies that would later lead to disease processes. They would make sure we were getting the proper supplements. Our diets, and lifestyles would be evaluated and we would be counseled on ways to improve our chances of remaining well. We would be evaluated, and corrected for structural imbalances that could lead to further injury later in life. We would have basic vital signs measured to be sure there were no signs of dangerous medical conditions impending. We would also see on occasion a medical practitioner that would do a more detailed physical and include occasional blood work to evaluate our path to optimal health. When such an occasion arose for emergency medical intervention, we would be referred to the appropriate health care provider. If medication was absolutely necessary, our natural health care provider would be well enough informed to counsel us on nutritional supplements to reduce the side effects and not interfere with the medication.
What a concept! Let's demand that now, it's up to you!!


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Tuesday November 24, 2009
Former Friends star Courteney Cox Arquette is returning to work on US series Cougar Town, after taking time off for a "family matter".
The actress will be back on set next Monday, following an already-planned break for Thanksgiving, her producers have confirmed.
Although no further details of the actress' sudden absence have been revealed, Courteney's spokesperson says she and her family are fine.
Of her latest small screen role, Courteney has said she loves playing divorced single mum Jules Cobb even more than she did starring as mother hen, and compulsive cleaner, Monica Geller in Friends.
Photo: � ABC


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[moving this to xml-dist-app. It's in regard to my earlier email; http://lists.w3.org/Archives/Public/xml-dist-app/2002Apr/0205 and also the issue of the HTTP 202 response code that the TBTF is considering.] On Mon, May 06, 2002 at 05:56:45PM -0700, Henrik Frystyk Nielsen wrote: > No, any HTTP 2xx status code other than 202 means that the request was > successfully received and processed. I don't believe so. RFC 2616 says; "10.2 Successful 2xx This class of status code indicates that the client's request was successfully received, understood, and accepted." Even ignoring what 10.2 says, the "2" has to mean the same thing for all 2xx response codes. Any extension response code can not be interpreted to mean "succeeded", since the code may mean something like 202. This interpretation is consistent with RFC 2616, from everything I've read, specifically 10.2. > 202 means received but processing > not completed. If this was not the case then I would never have been > able to buy anything using HTML forms as they have exactly the same > property. Actually, I wouldn't even have been able to do a GET because I > wouldn't know whether a 200 response was a representation of the > resource or just something else saying that processing is under way. > > The solution you point out is of course exactly the same as the "Ext" > mechanism in RFC 2774 but the reason that was introduced was because > many CGI scripts disregard the method name entirely. This is not really > the case here as you are not going to throw HTTP/SOAP messages at > arbitrary URIs. Is this an assumption that we can make? I was not assuming this, but I would agree that if we could assume this, that this issue goes away. But our charter says; "In particular, it must support distributed extensibility where the communicating parties do not have a priori knowledge of each other." I believe that what you suggest (maintaining the status quo) requires that there be a priori knowledge about which URIs identify SOAP services. > I don't think we need anything else other than another property in the > SOAP RR MEP describing the type of success. MB -- Mark Baker, Chief Science Officer, Planetfred, Inc. Ottawa, Ontario, CANADA. firstname.lastname@example.com http://www.markbaker.ca http://www.planetfred.comReceived on Monday, 6 May 2002 22:35:52 UTC
This archive was generated by hypermail 2.3.1 : Tuesday, 6 January 2015 22:01:20 UTC


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Please see http://www.w3.org/2009/sparql/wiki/Agenda-2011-04-12 . talk to you on Tuesday, Lee When joining please don't identify yourself verbally; instead, identify yourself to Zakim on IRC Date of Call: Tuesday April 12, 2011 Time of Call: 15:00 UK, 10:00 (East US) Dial-In #: +1.617.761.6200 (Cambridge, MA) Dial-In #: +18.104.22.168.79.03 (France) Dial-In #: +44.203.318.0479 (UK) Participant Access Code: 77277# (SPARQ) IRC Channel: irc.w3.org port 6665 channel #sparql ([irc:irc.w3.org:6665/sparql]) Web-based IRC (member-only): http://www.w3.org/2001/01/cgi-irc (Firefox IRC addon: chatzilla) Duration: 60 minutes Chair: Lee Feigenbaum Scribe: Alex (Scribe List) Link to Agenda: http://www.w3.org/2009/sparql/wiki/Agenda-2011-04-12 [edit] Agenda Admin (10min) PROPOSED: Approve minutes at http://www.w3.org/2009/sparql/meeting/2011-04-05 Comments Next regular meeting: 2011-04-19 @ 15:00 UK / 10:00 EST (scribe: Olivier) To Last Call Documents currently ready for review? Reviews ready? Documents ready for Last Call? Test status Approve entailment tests Action status (beginning with actions up to 397) Issue status [edit] Regrets Chime Andy Steve (at risk)Received on Monday, 11 April 2011 02:24:26 UTC
This archive was generated by hypermail 2.3.1 : Wednesday, 7 January 2015 15:01:04 UTC


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La Sirena
- Artist: Lua
- Genre: International
- Release Date: 9/7/2004
Lua Description-- There is a deep 'seeded' revolution going on-- each day as the world turns, countries become states, states become towns and towns become neighborhoods... technology brings the world in all it's beauty and rawness to our door step and it's in the evolution of our global music that Lua finds it's place. Lua does not claim to be of a specific tradition, though it is inspired by a blend of North American, Latin American and African influences; it is a unique sound that calls deeply to the human spirit for oneness. So if you choose to label us, that is what we would like for you to do. Our music is your music, so whatever it brings to you, let it be -- Nueva cancion, Cumbia jazz, Latin folk, Reggae, Luscious new world Soul --- be our guest. We would like to start a new label, a 'U-label,' because how can we label each individual's experience? How can we or anyone else for that matter label how you interpret the landscape of our dreams? Estela Bio: Her first experiences with music she describes as 'being touched by a force of nature. Even when I was a child I knew that I was a conduit for something much greater than myself, something that connects us all, that breaks through any tangible border or barrier that we as humans create.' When she sings you can hear that in her voice, The sounds of nature and the deepest parts of humanity seep through her sultry, soulful voice. She is the siren that calls you deeper into yourself, into those places where you can no longer hide. "Every sound that comes through me is not of me but is of the experiences that I have with this world community that I love-- it is their story that is my story. It is hard to see myself as a citizen of a particular country or place. I consider myself a citizen of the world and in the world I conduct myself as such.' Music is the voice of the people-- I want to inspire our world community to put down anger and sing-- for it is in each other that we find the deepest parts of God. My soul steps forward with great intention and inspiration to sing the song of my world. No more will I be silenced by my own fears'. Her songs are strong and deep. In her music, she can rip your heart right open with one phrase and in another have you flying through the canopies of heaven. Some of her songs are silly and super danceable and others are thought provoking and hard to pin down. 'I want my audiences to walk away feeling fed. I long to inspire community and connectedness. I want to reflect humanity's hunger for peace and a more unified world. I want us to find ways to shape our existence together, one note at a time.' Dave Bio: Dave is a highly accomplished bassist, composer and teacher, whose recording and performance credits include a broad spectrum of styles, from west African traditional music to jazz, funk, acoustic folk and various types of Latin and Caribbean popular music. He has often been inspired by the sensual movements of dancers throughout the world. 'I have always enjoyed composing dance music, but have never really gravitated towards any one specific style. It is my intention to write music that will inspire people to dance out the mythology of their dreamscape, wherever that leads.' Dave writes from a visionary place of poetic beauty. In his songs are images of the dreamer transforming the world through feats of imagination. 'Accepting the world as it is has never been one of my strong suits. When faced with a situation, whether an artistic dilemma or a real world problem, I can usually bend reality a bit to accommodate my intention. And if reality won't budge, I can always fall back on my imagination and my attitude towards the world, to shape things through song into spaces of great beauty. It is not enough to comment on the ugliness and cruelty in the world, nor to bicker with the perpetrators. We have to actually do things that make life more wonderful. And, you know, if that doesn't work, we can always write a song that expresses the beauty of what the world could become, and then at least we have some vision to nudge into being.'
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text: Monitoring HIV vaccine trial participants for primary infection: studies in the SIV/macaque modelWhitney, James Ba,b; Luedemann, Corinnea; Bao, Saranc; Miura, Ayakoa; Rao, Srinivas Sc; Mascola, John Rc; Letvin, Norman La,b,cAIDS: July 31st, 2009 - Volume 23 - Issue 12 - p 1453–1460 doi: 10.1097/QAD.0b013e32832b43d9 Basic Science Abstract Author Information Article Outline Objectives: The ability to detect and quantify acute HIV-1 infection prior to seroconversion would be an important tool for use in HIV vaccine clinical efficacy trials. We have utilized the SIV/rhesus monkey model to evaluate whether samples more easily obtained than peripheral blood might be used for intensive monitoring of vaccine trial participants. Methods: We have evaluated viral loads in peripheral blood, saliva, feces, and urine of five rhesus monkeys during primary SIVmac251 infection by quantitative real-time PCR. As an alternative to the direct monitoring of frozen samples, we have also developed a fully quantitative viral load assay utilizing dried blood spots. Results: Although all compartments were found to harbor viral RNA during primary infection, viral RNA could be detected in the peripheral compartments only when levels of plasma viremia exceed a threshold value of 104 RNA copies/ml. We found no direct correlation between viral burden in plasma and saliva, feces, or urine viral loads. Importantly, both dried saliva and whole blood spots can be used for viral detection. Quantitative whole blood or plasma spotting correlated well with viral burden in plasma during both the acute and set point phase of infection. Conclusion: Dried blood spots are amenable to rapid quantitative viral load testing. Whole blood spotting has a significant logistical benefit as it requires low blood volumes and no blood processing. Saliva or dried saliva spots or both are potential candidates for acute phase diagnostic screening. These studies indicate the feasibility of intensive monitoring of HIV-1 vaccine trial participants for virus acquisition in resource-limited settings. aDivision of Viral Pathogenesis, Department of Medicine, Beth Israel Deaconess Medical Center, USA bHarvard Medical School, Boston, Massachusetts, USA cVaccine Research Center, National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland, USA. Received 28 March, 2008 Revised 7 January, 2009 Accepted 23 January, 2009 Correspondence to Norman L. Letvin, MD, Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Research East Room 113, 330 Brookline Avenue, Boston, MA 02215, USA. Tel: +1 617 667 2766; fax: +1 617 667 8210; e-mail: email@example.com Abstract Introduction Methods Animals and SIV infection Sample collection and processing Viral RNA isolation, quantitative real-time PCR, and assay ... Analysis of SIV-specific antibody responses Statistical analysis Results Discussion Acknowledgements References Back to Top | Article Outline Introduction The ability to detect and quantify HIV-1 in humans prior to their seroconversion would contribute to the evaluation of vaccine efficacy in international HIV vaccine trials. Although the practical application of nucleic acid testing in HIV diagnostics has been well established [1,2], most currently employed methods for viral detection and quantification rely on venipuncture and processed blood plasma. These methods require trained personnel and modern, well equipped laboratory facilities that are not routinely available at resource-limited vaccine trial sites [3,4]. A strategy for screening at-risk human populations for HIV-1 infection using specimens from easily sampled compartments would provide a valuable tool for monitoring large cohorts of vaccinees. Numerous studies have shown the presence of viral RNA in a variety of compartments during the course of HIV infection, including the gut, saliva, breast milk and semen [5–10]. However, limited information is available regarding the correlation during acute HIV infection between viral RNA levels in the blood and in other compartments. Moreover, the presence, magnitude and duration of measurable viral RNA in various compartments (i.e. saliva, urine and feces) during acute HIV infection are not well described. Similarly, the predictive value of viral RNA levels in these different compartments has not been defined. Information of this type is critical for the development of rapid, high-throughput monitoring technologies for high-risk populations or vaccinees in HIV vaccine trials in resource-limited settings [3]. In the current study, we have employed the SIV/rhesus monkey model to determine if alternatives exist to routine venipuncture for monitoring HIV-1 infection in humans. Our aim was the detection and quantification of SIV RNA in simultaneously obtained samples of blood, saliva, urine and feces during the first 12 weeks following SIVmac251 infection. To evaluate an alternative to using frozen samples for analysis, we also developed a quantitative SIV assay using dried sample spots [11]. Back to Top | Article Outline Methods Animals and SIV infection This study enrolled and monitored five adult male rhesus monkeys (Macaca mullata) that were 4–6 years of age. All animals were screened and found to be negative for SIV and simian retrovirus type D prior to the initiation of this study. The monkeys were housed under Biosafety level-2+ conditions. All experiments were conducted in accordance with Institutional Animals Care and Use Committee standards. Prior to, and routinely after SIV infection, all animals were monitored by physical exam, complete blood count with differential, and T cell subset analysis. All animals were experimentally injected with a 1 ml bolus of SIVmac251 (2.11 × 105 viral RNA copies), directly into the saphenous vein. Productive infection of each monkey was confirmed by demonstration of seroconversion by western blot analysis (not shown). Back to Top | Article Outline Sample collection and processing Viral RNA levels were monitored in peripheral blood, saliva, feces and urine for the first 12 weeks after infection. Specimens were collected twice weekly for the first 6 weeks; all samples thereafter were collected every second week for a total duration of 12 weeks. Blood was collected into EDTA anticoagulant. Saliva was collected by cotton roll Salivette (Sarstedt, Nümbrecht, Germany). Both feces and urine were obtained by early morning clean pan collection. Blood was routinely processed by Ficoll-Hypaque overlay (Pharmacia, Uppsala, Sweden). Saliva was centrifuged immediately after collection at 4°C and according to the manufacturer's recommendations (Sarstedt). Fecal samples were immersed into equal amounts (w/v) of RNAlater (Ambion, Inc., Austin, Texas, USA) and homogenized by vortexing. Both fecal supernatants and urine were clarified by low-speed centrifugation and filtered through 100 μmol/l filters (BD Falcon, Franklin Lakes, New Jersey, USA) prior to aliquoting. Samples were spotted onto Whatman 903 protein saver cards. Forty microliters of saliva or 50 μl of whole unprocessed blood were placed as individual spots onto each card. Spots were dried for 2–3 h prior to being packaged in sealable sandwich bags and stored in the dark at room temperature, with desiccant until required for RNA extraction. Liquid samples were immediately frozen and maintained at −80°C until use. Dried sample spots were cut (with a 1–2 mm clean filter paper border) using disposable scissors. This method allowed extraction of the entire 50 μl spotted sample. Back to Top | Article Outline Viral RNA isolation, quantitative real-time PCR, and assay evaluation Viral RNA was routinely isolated from 200 μl or 400 μl of cell-free blood plasma, clarified saliva, urine or feces using the NucliSENS Isolation Kit (Biomerieux, Lyon, France) following the manufacturer's protocol. Excised sample spots were also processed using the NucliSENS Isolation Kit, with the exception that spots were incubated in NucliSENS lysis buffer for 2 h at room temperature with rocking, prior to further purification steps. To remove any contaminating cellular DNA, sample spot RNA preparations were DNase treated for 20 min at 37°C followed by heat inactivation at 75°C for 10 min prior to reverse transcription of template RNA. The SIV RNA standard was transcribed from the pSP72 vector containing the first 731 bp of the SIVmac239-Gag gene using the Megascript T7 kit (Ambion, Inc.). RNA was isolated by phenol-chloroform purification followed by ethanol precipitation. All purified RNA preparations were quantified by optical density. RNA quality was determined by Agilent bioanalyzer RNA chip (Agilent, Inc., Santa Clara, California, USA). Quantitative real-time PCR (qRT-PCR) was conducted in a two-step process. First, RNA was reverse transcribed in parallel with an SIV-gag RNA standard using the gene-specific primer sGag-R 5-′CACTAGGTGTCTCTGCACTATCTGTTTTG-3′ under the following conditions: the 50 μl reactions containing 1× buffer (250 mmol/l Tris-HCl pH 8.3, 375 mmol/l KCl, 15 mmol/l MgCl2), 0.25 μmol/l primer, 0.5 mmol/l dNTPs (Roche, Nutley, New Jersey, USA), 5 mmol/l dTT, 500 U Superscript III RT (Invitrogen, Carlsbad, California, USA), 100 U RnaseOUT (Invitrogen) and 10 μl of sample. Real time conditions were 1 h at 50°C, 1 h at 55°C and 15 min at 70°C. All samples were then treated with RNAse H (Stratagene) for 20 min at 37°C. All real-time PCR reactions used EZ RT-PCR Core Reagents (Applied Biosystems, Foster City, California, USA) following the manufacturer's suggested instructions under the following conditions: the 50 μl reactions containing 1X buffer (250 mmol/l bicine, 575 mmol/l potassium acetate, 0.05 mmol/l EDTA, 300 nmol/l passive reference 1, 40% (w/v) glycerol, pH 8.2, 0.3 mmol/l each of dATP, dCTP, dGTP, 0.6 mmol/l dUTP, 3 mmol/l Mn (OAc)2, 0.5 U uracil N-glycosylase, 5 U rTth DNA polymerase, 0.4 μmol/l of each primer and 10 μl of sample template. PCR reagents were assembled at room temperature and spun briefly to eliminate air bubbles. Following 2 min at 50°C, the polymerase was activated for 10 min at 95°C, and then cycling proceeded at 15 s at 95°C and 1 min at 60°C for 50 cycles. Primer sequences were adapted from those described by Cline et al. [12], forward primer s-Gag-F: 5′-GTCTGCGTCATCTGGTGCATTC-3′, reverse primer s-Gag-R: 5′-CACTAGGTGTCTCTGCACTATCTGTTTTG-3′ and the probe s-Gag-P: 5′-CTTCCTCAGTGTGTTTCACTTTCTCTTCTGCG-3′, linked to Fam and BHQ (Invitrogen). All reactions were carried out on a 7300 ABI real-time PCR system (Applied Biosystems) in triplicate according to the manufacturer's protocols. Preliminary experiments were done to evaluate the quantitation of blood plasma, saliva, feces and urine viral RNA levels and their correlation with known quantities of viral RNA added to compartment specimens sampled from SIV-uninfected monkeys (data not shown). To determine the linear range of the assay, RNA extraction efficiency, potential inhibition of reverse transcriptase activity, and the presence of compartment-specific RNAse activity, known quantities of serially diluted SIVmac251 viral stock were spiked into samples of peripheral blood, feces, urine or saliva that were recovered from three SIV-naive monkeys. No significant differences in extraction efficiency were observed between the blood and samples derived from other compartments (data not shown). Furthermore, using the extraction methods described above, we observed no significant inhibition of RT activity or diminution of input RNA as a consequence of RNAse activity present in any of the virus-spiked specimens (data not shown). All correlations between compartments were statistically significant. Correlations between blood and saliva ranged between 0.9843 and 0.9994 (Pearson's r), P = 0.006 to less than 0.0001, blood and urine, r = 0.9627–0.9991, P = 0.086 to less than 0.0001 and blood and feces, r = 0.9812–0.9965, P = 0.010 to less than 0.0003 as measured over eight orders of magnitude. These findings indicated, therefore, that it was appropriate to employ this technique to isolate viral RNA from study samples of peripheral blood, saliva, feces and urine from the newly infected rhesus monkeys. The analytical sensitivity of our quantitative RT-PCR assay was determined by using a series of purified gag RNA transcripts serially diluted and tested eight times; each individual run was conducted in triplicate. In these experiments, all dilutions containing four or more RNA copies could be detected in 100% of the samples. The assay detection limit was determined to be 60 copies of viral RNA when using 200 μl of blood plasma for the RNA extraction and transferring 10 μl RNA from 30 μl of extraction eluate. Similarly, the assay detection limit for 200 μl of saliva, urine or clarified feces was determined to be 125 viral RNA copies. Back to Top | Article Outline Analysis of SIV-specific antibody responses Binding antibodies to SIV proteins were analyzed by western blot. Commercially available SIV western blot strips (ZeptoMetrix, Buffalo, New York, USA) were probed with plasma samples and developed according to the manufacturer's instructions. Back to Top | Article Outline Statistical analysis Statistical analysis was conducted using commercially available software GraphPad Prism version 4.0b (GraphPad Software, Inc., San Diego, California, USA). Linear regressions were conducted to determine the significance of correlations. Significance of differences between groups was determined using Kruskall–Wallis analysis of variation (ANOVA) as appropriate. Probability values of P less than 0.05 were interpreted as significant. Back to Top | Article Outline Results qRT-PCR analysis of SIV-RNA showed that all compartments of the monkeys were viral RNA-positive during the acute phase of infection. The kinetics of viral RNA expression during this time was characterized by a period of undetectable RNA levels (below 60 copies/ml, triplicate samples), followed by a peak of viral RNA production, and then a set point nadir in the first 5 weeks after infection. The largest amount of viral RNA in the saliva, feces and urine was observed during the first 4 weeks of viral replication, coincident with the rise and fall of viral RNA levels observed in the peripheral blood (Fig. 1). Fig. 1 The median peak in viral RNA levels in the blood of these five monkeys (day 14 after infection) was 107 vRNA copies/ml of plasma in the peripheral blood, 105 vRNA copies/ml for both saliva and feces and 103 vRNA copies/ml in the urine. SIV RNA reached detectable levels in the peripheral blood as early as day 3 following infection. Viral RNA was detected in saliva in two of the five animals by day 10 and all animals by day 14. Both feces and urine of all animals had readily detectable levels of viral RNA by day 14. However, both feces and urine viral RNA levels fell to undetectable levels by 4 weeks postinfection. Viral RNA was detected in the peripheral blood and saliva of the monkeys over a prolonged period of time. Saliva viral RNA levels varied in each monkey, consistent with the plasma viral RNA set point values. During the postacute phase of infection (after day 35), viral RNA levels in saliva were diminished significantly. Occasional positive saliva samples were, however, observed in animals with high set point plasma viral RNA levels. To determine the total amount of detectable viral RNA in each of the sampled compartments, we calculated the total viral burden during acute viremia by area under the curve analysis (AUC). We found a significant difference (P = 0.003) between the AUC values in these monkeys in the peripheral blood and the other compartments. Blood plasma RNA levels reached 108 viral RNA copies/ml whereas the other compartments were found to be, on average, two orders of magnitude lower. We found that the levels of viral RNA did not differ significantly in saliva, feces or urine (Fig. 2). Fig. 2 In view of the established importance of plasma RNA levels for clinical disease progression in HIV-infected individuals, we were interested in exploring the association between this value and viral RNA levels in each of the other monitored compartments in these monkeys. No direct correlation was observed between plasma viral RNA levels and viral RNA levels measured in saliva, feces or urine (Fig. 3). Interestingly, however, these data demonstrate that viral RNA is detectable in the saliva, feces and urine only when plasma viral loads are in excess of 104 RNA copies/ml in the blood. This observation suggests that total body viral RNA levels must surpass a threshold prior to their detection in saliva, feces and urine. The viral RNA in these compartments may even represent 'spillover' from the blood. Fig. 3 To complement these studies, we were also interested in exploring the utility of assessing viral RNA levels in an easily and inexpensively stored format. We, therefore, evaluated the use of saliva and whole blood spotting onto Whatman 903 cards for monitoring and quantification of viral RNA. Dried sample spotting obviates the need for sample processing and requires low sample volumes. Moreover, control experiments assessing viral RNA extracted from dried blood spots (DBSs) up to 6 months after spotting showed that this method allowed for stable room temperature sample storage (data not shown). Similar results regarding the stability of DBS specimens have also been reported by other groups [11,13]. Although the risk of carrying over contamination from cutting devices is low [14], we chose to avoid this altogether and cut out the entire dried sample spot with a 2 mm border. This also provided the benefit of an exact sample volume for extraction (i.e., 50 μl) resulting in very low sample-to-sample variation in viral RNA quantification. In contrast to other sampling methods, the monitoring of viral RNA levels from dried saliva spots would allow the rapid and simple screening of large cohorts of vaccinees. Therefore, we monitored SIV RNA levels using dried saliva spots (Fig. 4a) and detected viral RNA as early as day 14 in three of the five animals, in all five of the animals on day 17 and in four of the 5 animals on day 21. We then compared these values to those obtained from the monitoring of frozen saliva samples. The association of spotted saliva viral RNA levels with the viral RNA levels obtained from frozen saliva was modest (r = 0.6253, P < 0.0001) (Fig. 4b.). These results likely reflect not only the diminished total viral RNA levels present in this compartment, but also the smaller sample volumes used in saliva spotting (50 μl) than in the monitoring of frozen samples (200 μl). Fig. 4 In contrast, dried blood spotting provided a robust method to evaluate both SIV-infection status and viral RNA levels. The longitudinal monitoring of SIV RNA levels from dried blood spotting from these monkeys is shown in Fig. 5a. In this format, SIV RNA was detectable in four of the five animals by day 7 and all of the five animals by day 10. The median viral load as measured from dried blood spotting was 0.2 log lower than values obtained from matched samples of processed frozen plasma (Fig. 5b). Importantly, the dried blood spotting based monitoring had a dynamic range comparable to monitoring in frozen plasma, from 103 to more than 108 viral RNA copies/ml of plasma. Moreover, the correlation of DBS viral load versus plasma viral load measurements was quite good (Fig. 5c) (r = 0.8319, P < 0.0001). Fig. 5 Therefore, between days 10 and 28 following infection, when plasma viral RNA levels were at least 104 copies/ml in all evaluated monkeys, we found that all blood spots were RNA positive. By comparison, 80% of saliva, 70% of feces and 50% of urine samples were SIV viral RNA-positive during this period. Detection of SIV RNA as dried saliva spots was approximately half (44%) compared with screening methods using fresh frozen saliva. The detection of SIV RNA after the acute phase peak of viremia (i.e. set point) dropped to near or completely undetectable in all compartments with the exception of the peripheral blood. During this period, dried blood spotting based methods were capable of detecting SIV acquisition in 90% of samples. The 10% of samples that scored negative were from a single animal that strongly controlled set point viremia. This animal routinely had peripheral blood plasma viral RNA levels of less than 500 copies/ml after day 50 of infection. Back to Top | Article Outline Discussion Vaccine studies in the SIV-macaque and simian human immunodeficiency virus-macaque models suggest that control of viral replication during primary infection is prognostic of long-term immunologic control of virus and clinical disease course. These studies show that the clinical effect of a T cell-based vaccine is most apparent during the first days following infection [15–17]. These studies demonstrate that diminished peak plasma viral RNA levels in vaccinated monkeys are associated with prolonged survival. Moreover, these studies indicate that peak plasma virus levels have greater prognostic value than set point values [16]. Monitoring of vaccinees in human HIV vaccine trials to capture data during peak viral replication would therefore be of enormous value [18]. However, obtaining such viral load data in humans during this early peak of viral replication has proven difficult. Clinical trial protocols include few time points over a large time span for infection determination, and detection of acute viremia is therefore easily missed. Sampling is particularly difficult in field trials in the developing world where vaccine recipients have typically been monitored only once every 3–6 months. The monitoring of vaccinees would have to be more intensive to capture the brief burst of viral replication during the course of primary HIV-1 infection. Furthermore, plasma specimens have been used for monitoring infection status in human clinical trials, and these specimens have been obtained by venipuncture. As these procedures require large numbers of highly trained personnel and an extensive laboratory infrastructure, they are very expensive [3]. Perhaps most importantly, human vaccinees, who have acquired HIV-1 often begin antiretroviral therapy immediately after diagnosis of infection. Set point viral load data from such individuals would be of limited value for judging the clinical outcome of vaccination. These individuals must therefore be dropped from any evaluation of the clinical utility of the vaccine intervention. In contrast, intensive monitoring for acute HIV-1 acquisition would allow early determination of infection status as well as valuable vaccine efficacy endpoints. The data in the present study regarding compartment-specific SIV RNA levels closely parallel well described SIV RNA levels in the peripheral blood and gut-associated lymphoid tissue during primary SIV infection. The increased levels of viral RNA measured in these compartments may represent a combination of local virus production and trafficking of infected cells into these mucosal compartments during acute infection [10]. A number of further modifications of these methodologies would be needed to employ these technologies in human clinical trials. Adopting this methodology for use in trials of human vaccine candidates would require extensive evaluation to avoid problems associated with diverse viral subtype detection [19]. An additional caveat is that the frequency of sample collection to detect acute HIV acquisition would need to be quite high. Thus, determination of the optimal collection frequency for DBS and dried saliva spot would need to be tailored to the specific size and location of each vaccine candidate trial. Sample frequency would be particularly critical if HIV monitoring was done using only saliva. The dynamic range of the viral RNA assay using saliva or dried saliva format was substantially less than the blood-based assays and likely would need improving. Finally, it is conceivable that the duration of exposure of viral RNA to host proteases and RNAses in vivo might be longer than that modeled in the present experiments, and, therefore, the results of these assays for human volunteers may be less sensitive. The present studies suggest that monitoring human vaccine recipients for a quantitative determination of peak viral replication during primary infection is feasible even in resource-limited settings. Both saliva and blood appear to be useful compartments for this monitoring. Moreover, dried saliva and dried whole blood spots should provide easily obtained specimens for quantitative acute phase HIV detection during acute infection or viral load testing during chronic infection. The cost of utilizing dried samples for HIV detection as compared with processing, storing and shipping frozen samples makes this format attractive for field vaccine trials. This study underscores the need to develop rapid, inexpensive technologies for quantitative viral RNA measurement using such dried specimens. DBSs, in particular, are becoming accepted as a method for viral load monitoring [13,20–25]. However, the present study indicates the feasibility of intensive, yet cost-effective monitoring of vaccinees for acute HIV-1 acquisition. Back to Top | Article Outline Acknowledgements This work was supported by the Intramural Research Program of the Vaccine Research Center, NIAID. J.B.W. was the recipient of a postdoctoral fellowship from the Canadian Institutes of Health Research (CIHR). J.B.W., S.S.R., J.R.M., and N.L.L. designed the study. J.B.W., C.L., S.B., A.M. and S.S.R. conducted the experiment and collected all experimental data. J.B.W. and N.L.L. analyzed the data. J.B.W., J.R.M. and N.L.L. wrote the manuscript.Back to Top | Article Outline References1. Pilcher CD, Fiscus SA, Nguyen TQ, Foust E, Wolf L, Williams D, et al. Detection of acute infections during HIV testing in North Carolina. N Engl J Med 2005; 352:1873–1883. Cited Here... 2. Busch MP, Hecht FM. Nucleic acid amplification testing for diagnosis of acute HIV infection: has the time come? AIDS 2005; 19:1317–1319. Cited Here... 3. Fiscus SA, Cheng B, Crowe SM, Demeter L, Jennings C, Miller V, et al. HIV-1 viral load assays for resource-limited settings. PLoS Med 2006; 3:e417. Cited Here... 4. Powers KA, Miller WC, Pilcher CD, Mapanje C, Martinson FE, Fiscus SA, et al. Improved detection of acute HIV-1 infection in sub-Saharan Africa: development of a risk score algorithm. AIDS 2007; 21:2237–2242. Cited Here... 5. Pilcher CD, Joaki G, Hoffman IF, Martinson FE, Mapanje C, Stewart PW, et al. Amplified transmission of HIV-1: comparison of HIV-1 concentrations in semen and blood during acute and chronic infection. AIDS 2007; 21:1723–1730. Cited Here... 6. Pilcher CD, Eron JJ Jr, Vemazza PL, Battegay M, Harr T, Yerly S, et al. Sexual transmission during the incubation period of primary HIV infection. JAMA 2001; 286:1713–1714. Cited Here... 7. Vernazza PL, Dyer JR, Fiscus SA, Eron JJ, Cohen MS. HIV-1 viral load in blood, semen and saliva. AIDS 1997; 11:1058–1059. Cited Here... 8. Shugars DC, Slade GD, Patton LL, Fiscus SA. Oral and systemic factors associated with increased levels of human immunodeficiency virus type 1 RNA in saliva. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2000; 89:432–440. Cited Here... 9. Henderson GJ, Hoffman NG, Ping LH, Fiscus SA, Hoffman IF, Kitrinos KM, et al. HIV-1 populations in blood and breast milk are similar. Virology 2004; 330:295–303. Cited Here... 10. Mattapallil JJ, Douek DC, Hill B, Nishimura Y, Martin M, Roederer M. Massive infection and loss of memory CD4+ T cells in multiple tissues during acute SIV infection. Nature 2005; 434:1093–1097. Cited Here... 11. O'Shea S, Mullen J, Corbett K, Chrystie I, Newell ML, Banatvala JE. Use of dried whole blood spots for quantification of HIV-1 RNA. AIDS 1999; 13:630–631. Cited Here... 12. Cline AN, Bess JW, Piatak M Jr, Lifson JD. Highly sensitive SIV plasma viral load assay: practical considerations, realistic performance expectations, and application to reverse engineering of vaccines for AIDS. J Med Primatol 2005; 34:303–312. Cited Here... 13. Brambilla D, Jennings C, Aldrovandi G, Bremer J, Comeau AM, Cassol SA, et al. Multicenter evaluation of use of dried blood and plasma spot specimens in quantitative assays for human immunodeficiency virus RNA: measurement, precision, and RNA stability. J Clin Microbiol 2003; 41:1888–1893. Cited Here... 14. Driver GA, Patton JC, Moloi J, Stevens WS, Sherman GG. Low risk of contamination with automated and manual excision of dried blood spots for HIV DNA PCR testing in the routine laboratory. J Virol Methods 2007; 146:397–400. Cited Here... 15. Barouch DH, Santra S, Schmitz JE, Kuroda MJ, Fu TM, Wagner W, et al. Control of viremia and prevention of clinical AIDS in rhesus monkeys by cytokine-augmented DNA vaccination. Science 2000; 290:486–492. Cited Here... 16. Letvin NL, Mascola JR, Sun Y, Gorgone DA, Buzby AP, Xu L, et al. Preserved CD4+ central memory T cells and survival in vaccinated SIV-challenged monkeys. Science 2006; 312:1530–1533. Cited Here... 17. Shiver JW, Fu TM, Chen L, Casimiro DR, Davies ME, Evans RK, et al. Replication-incompetent adenoviral vaccine vector elicits effective antiimmunodeficiency-virus immunity. Nature 2002; 415:331–335. Cited Here... 18. Pilcher CD, Shugars DC, Fiscus SA, Miller WC, Menezes P, Giner J, et al. HIV in body fluids during primary HIV infection: implications for pathogenesis, treatment and public health. AIDS 2001; 15:837–845. Cited Here... 19. Perrin L, Pawlotsky JM, Bouvier-Alias M, Sarrazin C, Zeuzem S, Colucci G. Multicenter performance evaluation of a new TaqMan PCR assay for monitoring human immunodeficiency virus RNA load. J Clin Microbiol 2006; 44:4371–4375. Cited Here... 20. Garrido C, Zahonero N, Fernandes D, Serrano D, Silva AR, Ferraria N, et al. Subtype variability, virological response and drug resistance assessed on dried blood spots collected from HIV patients on antiretroviral therapy in Angola. J Antimicrob Chemother 2008; 61:694–698. Cited Here... 21. Fiscus SA, Brambilla D, Grosso L, Schock J, Cronin M. Quantitation of human immunodeficiency virus type 1 RNA in plasma by using blood dried on filter paper. J Clin Microbiol 1998; 36:258–260. Cited Here... 22. Mwaba P, Cassol S, Nunn A, Pilon R, Chintu C, Janes M, Zumla A. Whole blood versus plasma spots for measurement of HIV-1 viral load in HIV-infected African patients. Lancet 2003; 362:2067–2068. Cited Here... 23. Alvarez-Munoz MT, Zaragoza-Rodriguez S, Rojas-Montes O, Palacios-Saucedo G, Vazquez-Rosales G, Gomez-Delgado A, et al. High correlation of human immunodeficiency virus type-1 viral load measured in dried-blood spot samples and in plasma under different storage conditions. Arch Med Res 2005; 36:382–386. Cited Here... 24. Ayele W, Schuurman R, Messele T, Dorigo-Zetsma W, Mengistu Y, Goudsmit J, et al. Use of dried spots of whole blood, plasma, and mother's milk collected on filter paper for measurement of human immunodeficiency virus type 1 burden. J Clin Microbiol 2007; 45:891–896. Cited Here... 25. Kane CT, Ndiaye HD, Diallo S, Ndiaye I, Wade AS, Diaw PA, et al. Quantitation of HIV-1 RNA in dried blood spots by the real-time NucliSENS EasyQ HIV-1 assay in Senegal. J Virol Methods 2008; 148:291–295. Cited Here... Keywords:acute HIV infection; HIV-1 infection monitoring; simian immunodeficiency virus © 2009 Lippincott Williams & Wilkins, Inc.


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New Year's Receptions - February 2017
In February, the German-Australian Chamber was pleased to hold three New Year's Receptions in Brisbane, Melbourne and Sydney. Each event was a fantastic opportunity for members and friends to meet with the chamber staff and board, network and hear about the exciting functions during the 40th anniversary.
Brisbane event report
Melbourne event report and photos
Sydney event report and photos
Cruise with the Europeans - November 2016
Cruise with the Europeans - November 2016
Following many successful years, nearly 700 members and friends of 15 European Chambers joined the 16th Cruise with the Europeans on Thursday, 10 November 2016. The MV Sydney 2000, waited at King St Wharf, Jetty No. 1 for everyone to board.
3rd Annual German Chamber & Allianz Golf Day - October 2016
3rd Annual German Chamber & Allianz Golf Day - October 2016
In a month where the weather can only be described as inconsistent und unpredictable, we were fortunate enough to be greeted at the beautiful Huntingdale Golf Club by a nice and moderate spring day for our 3rd Annual German Chamber & Allianz Golf Day on the 24th of October.
4th Annual Gala Dinner - September 2016
4th Annual Gala Dinner - September 2016
On Friday, 16 September 2016, the German-Australian Chamber of Industry and Commerce had the pleasure of inviting its members and friends to its 4th Annual Gala Dinner. With the event sold out weeks in advance and nearly 200 people from Chamber members, German and Australian businesses, and high-level political representatives in attendance, the James Cook Ballroom at the InterContinental Sydney was filled to the very last seat.
Christmas in July in Melbourne and Sydney - July 2016
Christmas in July in Melbourne and Sydney - July 2016
Lebkuchen, Glühwein and Christmas songs? It must not necessarily be December in order to enjoy these German delicacies. The participants of our Christmas in July events in Sydney and Melbourne could also taste them and enjoy the winter ambience.
President and Ambassador's Farewell - June 2016
The German-Australian Chamber had the great pleasure of hosting a farewell for Former Chamber President Ms Lucy Hughes Turnbull AO and German Ambassador to Australia H.E. Dr Christoph Müller.
Taking place at the beautiful Chiswick function room at the Art Gallery of New South Wales, this special event was made possible with the kind support of the Embassy of the Federal Republic of Germany. Ms Turnbull and Dr Müller are two very influential figures who have done much to improve and build on the German-Australian bilateral relationship.
Event Series: The new German Australian Double Tax Agreement
Event Series: The new German Australian Double Tax Agreement
The German-Australian Chamber launched a topic related event series this year, kicking it off with three events about the German Australian Double Tax Agreement. These informative events were hosted by three different Chamber-member companies and included presentations about the new tax agreement. Members had afterwards time to address their questions, accompanied by delicious drinks and food.
Breakfast Seminar at BDO in Brisbane
Evening Forum at EY in Melbourne
AGM & Economic Outlook - May 2016
AGM & Economic Outlook - May 2016
What an inspiring and interesting day Thursday has been. We welcomed roughly 90 Members for our AGM & Economic Outlook, which was kindly hosted by Deutsche Bank in Melbourne on 19 May 2016.
Delegation Trip to Adelaide - March 2016
Delegation Trip to Adelaide - March 2016
At the beginning of March, the German-Australian Chamber was privileged to facilitate a three-day delegation trip to Adelaide, highlighting South Australia's growing status in scientific research and unique role as the "defence state". South Australia has one of the largest manufacturing and defence industries in the country and happens to have the only state government with a dedicated Minister for Defence Industries. In particular, Adelaide is home to a large proportion of Australia's defence sector, which contributes more than $1 billion to South Australia's Gross State Product.
Visit of Prof Dr Maria Boehmer, Minister of State - October 2015
Visit of Prof Dr Maria Boehmer, Minister of State - October 2015
On 21 October, the German-Chamber of Industry and Commerce, together with Premium Partner SAP, hosted a roundtable discussion followed by an exclusive luncheon on the occasion of the visit of Professor Dr Maria Boehmer, Minister of State and head of the German side of the Australia-Germany Advisory Group (AGAG). In the evening President Lucy Hughes Turnbull AO hosted a Reception at her residence.
President's Delegation to Germany - July 2015
President's Delegation to Germany - July 2015
Berlin Delegation: What's next in the bilateral business relationship?
Full article in the German-Australian Business News Magazine
Berlin-Sydney Online Roundtable - May 2015
Berlin-Sydney Online Roundtable - May 2015
On Thursday 7 May, around 70 invited guests of the German-Australian Chamber of Commerce and The Committee for Sydney made their way to the Museum of Contemporary Art in The Rocks, to participate in the first Berlin-Sydney Online Roundtable and discuss the future of these two extraordinary cities.
German-Australian Business Reception - Nov 2014
German-Australian Business Reception - Nov 2014
The German-Australian Chamber of Industry and Commerce hosted a Business Reception in honour of H.E. Dr Angela Merkel. Her program in Sydney followed the G20 Leadership Summit in Brisbane. This reception was part of the first visit of a German Chancellor to Australia in more than 15 years.


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Grace, God's life in ours, is a gift from God. Finding it is a sure sign of God's presence in our lives. It changes us, converts us, so that God can use us to change others and society as a whole. Sometimes something in itself evil, like sickness, can become an occasion for God to act in our life so that he can bring good out of evil. I had that experience this past year, when my own illnesses were the occasion for so many to turn to the Lord in prayer and when I experienced anew our relation to one another in the Lord through a vast network of prayer. Prayer opens our lives to God so that he can heal us, spiritually and physically.
God expects us, however, to cooperate with his action in our lives by setting aside time for prayer each day and by using ordinary means to protect our spiritual and physical well-being. Protecting physical health is a social and therefore a political issue as well as a spiritual exigency. Because so many people in our state do not have the protection of ordinary means to preserve good health and to cure disease, proposals have been put forward in recent years to address the issue of inadequate health insurance.
How major is the challenge? In 2005, there were 44.8 million medically uninsured people in the United States. Nationally, nearly 18,000 deaths annually are attributable to the fact that people do not seek medical care in time because they are uninsured. In our state, we fare a little better than other states. 24.7 percent of Illinois children lack health insurance, while the national average is 25.6 percent. 24.1 percent of Illinois children do not have a doctor, while nearly 35 percent of all children in the U.S. do not have one. A successful program for children (Kids' Care) has somewhat eased the situation in Illinois in recent years, but the major challenge of providing adequate healthcare coverage for all remains.
The Church looks at this situation as a moral problem. Having access to basic healthcare is a right that flows from each human person's inherent dignity. Rights imply duties. Whose duty is it to provide access to healthcare? Catholic social teaching tells us that we all, collectively, need to see to it that there is access to fundamental healthcare. In the United States, many employers have provided health insurance, and employees have paid deductibles and co-pays. Because of increasing costs, the percentage of employers providing health insurance has decreased, even as the employees' contribution to their employer sponsored health plan has increased. The government also has provided access to healthcare through social programs such as Medicare, Medicaid, SCHIP and veterans' benefits. These programs, however, do not address the needs of the growing number of people not covered by their benefits. In a society marked by clear division between the rich and the poor, our faith recalls the story of the Last Judgment (Mt. 25: 31- 46) and instructs us to put the needs of the poor and vulnerable first.
Every program that aims to cover the uninsured and help the underinsured carries a price tag. How will it be paid for? The Church cannot and should not offer technical solutions to this question, although the problem is before us every day in the emergency rooms of Catholic and other hospitals. These are filled with those who have no other place to turn for basic healthcare. They are not turned away, but the emergency room is not the place to address healthcare issues that should not have developed into emergencies. Catholic institutions are the largest, non-profit private provider of healthcare in Illinois and the United States. The Church follows this debate carefully because she is interested in the sick and the poor and also in the health of her own institutions.
The question of how to provide healthcare to all in Illinois has been and will continue to be debated in the General Assembly. The principle is clear, the goal is laudable, but finding the means is a political and budgetary problem of great magnitude. There are many details for Governor Blagojevich and our elected representatives to decide, but they should be applauded for their willingness to address this problem that has been before us for so long.
There is a question, finally, of health beyond institutional healthcare. A recent UNICEF report places the United States twentieth in the world in the quality of children's health, below almost every European country but Britain. This difference runs through every economic class, the rich as well as the poor. Twenty five hundred years ago, the philosopher Plato wrote in the third chapter of the Republic that an ideal society doesn't need many doctors, because the people are basically healthy. (He also argued that the ideal society doesn't need many lawyers, because the people are basically just.) How we live day after day affects both our spiritual and physical health. The medical statistics tell us more than just how many sick people live here; they also indicate how well we are as a people, as a society.
I hope that God's grace may keep us healthy in body and spirit. I pray as well that the Holy Spirit may guide our elected officials in the current debate about how to provide basic healthcare for all. God bless you.


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Julien Bayle
Opening on 30th May in Marseille / Gallery Karima Celestin
Daily traversed by more or less interpretable signals using equipment more or less sophisticated as our cell phones or our radios, we can only get the meaning of a very small part of them, finally.
These digital or analog signals carrying information are transcoded and transported by electromagnetic waves traveling in our world meshed by antenna systems, networks splitters, filters and other as invisible as necessary equipment in our lives often reduced to propagation of messages.
signal.interrupt is a generative sound and visual installation that visits the various forms of modulation and signal creation itself from pseudo-random generators that displays programmed shapes and generates continuously sound and visuals, exploring the modulation frequency of amplitude, tone generation by random wave table, among other processes.
signal.interrupt is based on an algorithm that generates digital bits 0 or 1, which are stored in real-time in temporary memory and read as if they were full-fledged sound signals. The process of generating, storing and playback is not made constant and identical speed and thus causes an infinity of possible leaving room for a set of possible disturbances.
signal.interrupt also analyzes in real-time the audio signals produced and thus create visual forms with a strong minimalistic aesthetic parti-pris. From the smoothed waveform to the most disturbed one, signal.interrupt provides an immersion in the core of signal generation, signal processing processing, keeping a place for triggered random, and sometimes deliberately uncontrolled that can also lead to a total disruption.
signal.interrupt therefore attempts to show through different moments and phases that if we are able to recognize patterns, repetitions in these signals, it is just because they are constantly disturbed and interrupted; indeed, the sine qua non condition for creating an information from a medium being the fact that we can change the medium parameters itself.


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Origins: The Gateway Gallery
The Gateway Arts Foundation has its origin as a public arts collection consisting of many residential apartment buildings and one motel in the area known as the Gateway To The Cultural Corridor.
Through on-going efforts Gateway transformed previously tagged buildings into "canvases" for murals. All murals were created by local artists and every artist received compensated for each work of art.
This project, "one of a kind" for the city of Las Vegas, represented a true cooperation between property owners, the city of Las Vegas, and Las Vegas artists. This continuing art collection began as a solution to the problem of tagging in this area and its positive results are evident in the form of neighborhood beautification and pride.
For his time, energy, and financial commitment, Wells Fargo and Channel 13 honored Gerald Duskin with the Jacqueline Kennedy Inspired Jefferson Award for Outstanding Neighborhood Volunteer.
The second phase of this project, "Alley Art", saw selected students from The Stupak Center working with young local artists to paint the rear of many buildings in the alley behind Fairfield Street.
These students loved the opportunity to create artwork in their neighborhood and proudly show their friends their contributions. Once again, committing time and volunteerism to a true vision has given young people a way to use their talents and paint in a positive way.
The Foundation Today
A natural transition from the visual arts program is our dedication to the wonderful talents of the aspiring and professional performing artists in fabulous Las Vegas.
To this end Gateway provides "Hattie's House" free of charge to music and theater teachers to be used for their students to perform and for recitals. Hattie's House is a living tribute to Camille's late mother.
Regularly scheduled monthly events feature a variety of performances by Las Vegas artists. Ticket sales for these events compensate the performers and benefit the Gateway Scholarship fund for young Las Vegas artists.
The Foundation seeks to continually make a huge impact on the cultural community of Las Vegas. We invite you to join the Gateway Arts Foundation by attending one of our great events, contributing to the scholarship program, or remembering Gateway with your thoughtful donation.
Gateway Arts Foundation Board Members
Retired CEO
Woodley Clubhouse, Inc.
Retired Owner/Operator
My Construction, Inc.
Singer, Artist, and Costume Designer for the past 20 years.
Director, Jude 22 – Senior Nutrition Center, Las Vegas
Retired Tax Professional
Board member
Caesar's Executive


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Engineers, land surveyors and related professionals provide us with clean air, clean water, safe roads, well-designed buildings and a better quality of life. Engineers supply solutions for better living.
Sponsor Association Outreach on the Web
Check out these fun sites to learn more about engineering and how YOU can get involved!
Click Here to download an Outreach Guide
Click Here to request a brochure, an interview with an engineer, or a classroom visit.
Image below posted courtesy of Mrs. Chelsea Bailow's second grade class, Fiske School, all rights reserved.


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Today is a snow day. Snow started falling yesterday afternoon and by 5:30 this morning, the school called to declare a two-hour delay. Two hours later, they called again to cancel. So I slept in. This is my typical response to a snow day, because most days I'm up by 6:00. I like sleep and will find excuses to indulge. The snow's been lightly falling all day, a soft blanket of beautiful white, decorating bare branches and pines with a fleecy white veil. My last check revealed a total of six inches—and it's still coming.
Yesterday when the snow started, I was at a doctor's appointment about twenty-five miles from home. After lunch with my husband, I drove home, noting the blinking highway signs reading, "Caution: White-out conditions." In the distance, the valley looked fuzzy—a sure sign of snow, rain or other precipitation. Still, I continued on. After all, how bad could it be? Not more than a mile down the road, I encountered the white-out. It was like someone drew a line, and that was where the mayhem started. Blowing snow, decreasing visibility, gusting winds. Fun, fun. In our area, we've had multi-car pile-ups on the interstate in similar conditions. At the next exit, I drove to the old mountain road which everyone used before the interstate went in. It's longer, but the trees block the wind and reduce the blowing snow that make I-80 so dangerous. Instead of a white-out, I faced untreated roads and slush-filled tracks. I followed the twists and turns up the mountain while my traction control engaged and disengaged. I'm blessed I didn't get stuck. After a quick stop at Dollar General for the requisite milk and water (we already had enough bread), I hurried home.
If I had to do it over again, I'd make the same choice. But it made me remember why I hate driving in snow.
I've lived in Pennsylvania my whole life and in this small town for most of it. I'm not sure how I'd handle living in another part of the country where hurricanes or tornadoes or earthquakes are the norm. Blizzards and heavy snowfall seem easier to handle. Our preparation checklist looks like this:
- Buy enough gas for a generator, if you own one.
- Make sure your vehicles have full tanks.
- Check your pantry for a stash of canned goods.
- Cancel any appointments that may have been scheduled.
- Buy milk, bread, and water and "hunker down."
We're hunkered down now. I'll let you know when we get out


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"My daugher is 12 and had her first seizure last yr. Her EEG came back and she was given meds since it showed something. She was watching TV at the time it happened. But not long after ended up having her period. I noticed her mood swings..."
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Appeals court blocks Clarett's bid for draft
By Ron Borges Globe Staff / April 20, 2004
- |
Maurice Clarett's end run was thrown for a loss yesterday. The Ohio State running back, who is trying to buck the NFL's draft restrictions on underclassmen, was rebuffed by the United States Court of Appeals for the Second Circuit, which barred him and Southern California wide receiver Mike Williams from entering Saturday's NFL draft. The ruling by the three-judge panel ... (Full Article: 631 Words)
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Sara Jaye Weiss/StartraksPhoto.com
Sara Jaye Weiss/StartraksPhoto.com
Dear Ted:
Is Hugh Jackman really the great guy that he seems to be? The all-around great husband and great father. Does he have any skeletons or Vices? Also has Toothy Tile ever been photographed with his beard?
Dear Naughty and Nice:
Just because the X-Men star may also be a Vice star—which he is—doesn't mean he's not the nice dude he appears to be. Trust me, I adore Mr. Jackman and I've known about his Vice since like...forever. As for Toothy, T.T. has been snapped with beards, of course. That's the point!
Dear Ted:
Thank you for starting to write about sports B.V.'s again instead of constant Twilight nonsense! Jay Cutler has to be Pepper Harthman, duh. There have been rumors about why he and Kristin Cavallari split the first time. Maybe if these Twi-hards picked a (sports) team for goss, they'd learn about some real Vicey behavior instead of obsessing over one relationship on your boards all day long. Boring!
Dear Pep Assembly:
Glad you're loving the sports stuff, Sue, I'll definitely try to keep it up for ya—especially since there's so much naughty dish to spill about those dudes when they're off the field. And while Cutler might have a few secrets that have yet to come out (even from fiancée K.Cav), he's not Harthman.
Dear Ted:
Is Chuck Finger-Dingle Zooey Deschanel? P.S. Am new to the Awful Truth but totally addicted! Love it!
Dear New Girl on New Girl:
Welcome, babe, and great first guess, but hate to tell ya Zooey is not our lady-lovin' Vicer. The New Girl chick hasn't earned herself a moniker just yet. But now that she's single and hotter than ever, who knows what'll happen.
Dear Ted:
Did you see the Blind Gossip item about the guy who asked a famous NFLer to say "hello" to his 85-year-old grandma on his way out of a restaurant? The player responded by telling them to eff off? Why is this a secret? That D-bag makes millions working in a fan-dependent "business." He behaved outrageously in a public space. I would have named him in a second! In a situation like this, why protect his privacy?
Dear Take It With a Grain of Salt:
Yes, I heard about the par-tick bit some time ago when it was just some rumblings around town. It's blind because it could tip off a lawsuit, really, but with that said: I don't really entirely buy it. I know which baller it's supposed to be though.
Dear Ted:
Thanks for the clues on Vicey Charlotte! They were really helpful, but now, I've got to ask you, what has Portia Vajazzle been up to lately, any helpful hints? You never answered many questions about her.
Dear All That Glitters:
Portia hasn't really been up to anything lately, at least nothing too juicy. Maybe it's because she's between gigs right now. Usually she's up to her naughtiest when she's got a movie to pimp or a gig to land.
Dear Ted:
So yesterday you mentioned that a celeb can't have more than one Vice name. Would you mind letting us in on some differences between the celebs behind Twyla Babe-Sucker and Terry Tush-Trade then?
Dear Battle of the Broads:
Twyla is much more conniving than Terry. She's probably hotter too.


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Chipper Jones, who has played for nearly two months with a torn meniscus in his right knee, has been placed on the disabled list and will undergo arthroscopic surgery layer today.
Jones said he hopes to miss only two to three weeks recovering from surgery, but some are saying that he could be out as long as a month because of age and uncertainty.
"I just didn't want to play through it all summer and have something go wrong in September and have to miss the playoffs," Jones said. "It's killing me to hit right-handed, so we decided to get it done today."
Jones had been treated with cortisone shots, but the pain kept getting gradually worse. "The shots didn't help me at all," Chipper said.
Bad news for the Braves, but good news for the Mets?
As the Mets look to make up ground in the Wild Card race, their chief nemesis for the top spot is clearly the Braves. Now is the time for the Mets to turn on the after-burners and chop away at the 6.5 games separating them from the Braves who currently hold the Wild Card lead.
Only four team now stand between the Mets and a Wild Card spot. A month ago there were ten teams separating the Mets from the top spot.
They have made up an enormous amount of ground, and no matter how good the opponent, they manage to eke out wins despite their best players out with injuries.
This could be the biggest break Mets have gotten all season. I'm calling it their Big Bang Theory.
Standings graphic by MetsBlog


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- $249,900 price
- LO9728098 mls id
- Year Built: 2016
- Lot Size: 6.01 acres
- Neighborhood: Rock Division
- Status: Active
Six Acres with Road Frontage on Route 9. Located directly to the left of the new Wine Reserve at Waterford. Mostly wooded lot with numerous possibilities. NO HOA means even more possibilities. Waterford is a very desirable area of Loudoun County.
- Site Link
- Style Other
- Roof Type Unknown
Listing Agent: Mark Malick
Courtesy of: Franklin Realty LLC
Office Phone: +17034375580
Information deemed reliable but not guaranteed.
Updated: 17th May, 2017 4:26 PM.
38490 CHARLES TOWN PIKE Waterford, VA
Real Estate | ARLnow Powered by Placester


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Kardashian Sisters Prove The Hollywood Gossip Correctby Hilton Hater at . Comments
The Kardashians are getting way too predictable.
Earlier today, we posted a pair of tabloid covers that called out the relationships of Kim, Khloe and Kourtney. We said the stories were likely planted by their publicists, so the siblings could respond later, garner sympathy from their fans and remain a major part of the entertainment news cycle.
It only took a few hours for the sisters to prove us right. Point, THG!
First, Khloe took to her blog and wrote:
"My sisters and I had a great laugh this morning. Star and InTouch decided to attack our relationships. Apparently I'm heading for divorce, Kim was dumped and Kourt was duped... Wow, sad week for us Kardashian sisters, and how coincidental that this all happened at the same time!"
This is how the Kardashians roll: They greenlight negative stories to tabloids, whine about them to the press, boost the sales of said tabloids, make new headlines for themselves... and then do it all again a month later.
Kim then chimed in from her blog:
"OMG! Have you guys seen the front pages of In Touch and Star today!? Do NOT Trust the Media!!!!!!"
In closing, Kourtney, brought this perfect storm of publicity for the family home:
"Week after week Scott and I have been on the cover of In Touch with stories that make us laugh.... We have been blessed with the most amazing, beautiful baby boy and couldn't be happier. And saying that Scotts initials are STD and that he had a dogtag because he was proud of it... his initials are SMD, but nice story, idiots."
On one hand, you have to be digusted that so many people still buy into the Kardashian myth (i.e. that there's an ounce of unscripted drama to anything they do and say).
On the other hand, you really need to be in awe of the way they've taken advantage of a culture that's so obsessed with people whose biggest accomplishment to date is to focus the camera while Ray J does his thing.