text:
- Research
- Open Access
E2A suppresses invasion and migration by targeting YAP in colorectal cancer cells
- Hongchao Zhao†1, 2,
- Ao Huang†1, 2,
- Pu Li†1,
- Yingjun Quan1, 2,
- Bo Feng1, 2,
- Xuehua Chen1,
- Zhihai Mao1, 2Email author,
- Zhenggang Zhu1 and
- Minhua Zheng1, 2Email author
© Zhao et al.; licensee BioMed Central Ltd. 2013
- Received: 12 September 2013
- Accepted: 25 November 2013
- Published: 26 December 2013
E2A gene, which encodes two basic helix–loop–helix (bHLH) transcription factors E12 and E47, has been identified as regulator of B lymphoid hematopoiesis and suppressor of lymphoma. E47 protein was found to decrease E-cadherin expression and induce epithelial-mesenchymal transition (EMT). However, the role of E2A in colorectal cancer (CRC) metastasis is still elusive.
qRT-PCR and semi-qRT-PCR were performed to determine mRNA level of E2A in CRC specimens and colorectal cancer cells. RNAi was employed to downregulate E2A expression and subsequent protein level change was evaluated by immunoblot. Cell invasion and migration capacity were detected by transwell assay using cell culture inserts with or without basement membrane matrix, respectively.
E2A expression was decreased in metastatic CRC tissues. Invasion and migration assays showed downregulation of E2A increased metastatic capacity of CRC cells while forced expression of E12 or E47 could offset this effect. Both E12 and E47 suppressed EMT induced by E2A downregulation. Moreover, Yes-Associated Protein (YAP) was a downstream target of E2A and suppression of YAP inhibited the pro-migration/invasion of E2A deficiency.
Our results suggest that E2A suppresses CRC cell metastasis, at least partially if not all, by inhibiting YAP expression.
- Metastasis
- Colorectal cancer
Colorectal cancer (CRC) is one of the most prevalent malignancies worldwide, ranking the third of all cancer-related deaths, and distant metastasis is the major cause of deaths for CRC patients[1]. These secondary tumors arise as the result of a multi-step process which begins when cancer cells within primary tumors break away from the microenvironment and invade through the basement membrane[2]. Although many metastasis-related genes have been identified in CRC[3], the complicated molecular mechanism of CRC development and progression is not yet fully understood.
The E2A gene encodes two basic helix–loop–helix (bHLH) transcription factors, E12 and E47, by means of variant splicing[4]. The E2A proteins (E12/E47) belong to the class I bHLH family and regulate expression of target genes by binding DNA with tissue-specific Class II HLH proteins, either as homodimers or as heterodimers[5–7]. Previous studies have shown that E12/E47 proteins are distributed widely in most adult tissues although at different expression levels[8, 9]. Both proteins are indispensable for some normal tissue development while deficiency or aberrant expression of E2A could lead to tumorigenesis. For instance, E12/E47 proteins are required for B-cell differentiation and Ig gene rearrangements[10]; two aberrant fusion proteins, E2A-HLF and E2A-PBX1, lead to pro-B cell acute lymphoblastic leukemia (ALL) and pre-B cell ALL[11]. In addition, targeted disruption of the E2A gene leads to thymic lymphomas, suggesting that E2A gene products can act as tumor suppressors[12, 13]. Specifically, the expression of epithelial maker E-cadherin was repressed upon E12/E47 binding to the E-pal element, a palindromic sequence between -75 and -86 in the E-cadherin promoter[14].
In colorectal cancer, ectopic expression of the E47 efficiently sequestered endogenous Id proteins from Id-bHLH heterodimers, leading to growth arrest of the cells[15]. However, it still remains unknown whether E12/E47 proteins affect CRC cells metastasis or not. In the present study, we demonstrated that E2A down-regulation was required for the development of metastasis while ectopic expression of E2A in CRC cells could suppress invasion and migration in vitro. Furthermore, we identified, and validated YAP (Yes-Associated Protein) as a novel downstream target of E2A.
Cell culture
Human colorectal cancer cell lines, SW480 and Caco-2, were purchased from American Type Culture Collection (ATCC). SW480 was cultured in Leibovitz' L-15 Medium (Corning Cellgro®, Manassas, VA, USA) with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA), and Caco-2 in Minimum Essential Medium (Corning Cellgro®) with 20% FBS. Cells were maintained at 37°C/5% CO2 in a humidified incubator.
Clinical specimens
The clinical research protocol was approved by the Ethical Committee of Ruijin Hospital, Shanghai Jiao Tong University School of Medicine. Seventy-five surgical specimens of primary CRC tumors were obtained from Shanghai Minimal Invasive Surgery Center (2012–2013) with written informed consents given by all patients prior to surgery. Inclusion criteria were: without neoadjuvant chemoradiotherapy; resectable colorectal cancers; without evidences of primary tumors of other organs; able to be interviewed during the follow-up. Fresh tumor tissues were harvested immediately after samples' dissection, snap-frozen in liquid nitrogen, and preserved at -80°C. The pathologic classification/staging of tumors was performed in accordance to the Cancer Staging Manual from the International Union Against Cancer (7th edition, 2009). Tumor samples were classified into two groups: tumors with metastatic sites and tumors without metastases, based on the status of lymph node and/or distant metastasis.
Protein extraction and immunoblot
RIPA buffer (Solarbio, Beijing, China) containing protease inhibitor cocktail (Roche Applied Science, Basel, Switzerland) was used to extract total tissue and cell proteins, according to manufacturer's instruction. Immunoblot was done according to the standard protocol, with primary antibodies against E2A (Santa Cruz, Dallas, Texas, USA), vimentin (Santa Cruz), E-cadherin (Cell Signaling Technol., Danvers, MA, USA), β-catenin (Cell Signaling Technol.), YAP (Cell Signaling Technol.) and MMP-9 (Cell Signaling Technol.). Goat anti-mouse or goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP, Pierce, Rockford, IL, USA) was used as the secondary antibody. Chemiluminescent signals were visualized using SuperSignal West Pico Chemiluminescent Substrate (Pierce) and the signal intensity was analyzed using the Image Lab™ Software Version 4.0.1 (BIO-RAD, Hercules, CA, USA). The experiments were performed in triplicate with GAPDH (Kangchen, Shanghai, China) as endogenous control.
Cells growing on slides (Millipore, Billerica, MA, USA) were fixed with 4% paraformaldehyde (PFA, Sigma-Aldrich, St.Louis, USA) and permeabilized for 10 min using 0.1% TritonX-100/phosphate buffered saline (PBS). Non-specific antigens were then blocked with 3% BSA in PBS-T (0.2% Tween 20) for 1 h at room temperature. After washing, cells were incubated with primary antibodies against vimentin (Santa Cruz), E-cadherin (Cell Signaling Technol.), β-catenin (Cell Signaling Technol.) in 4°C overnight, followed by incubation with Alex Fluor® 555 anti-Rabbit IgG (Cell Signaling Technol.) for 2 hours at room temperature. Images were examined and captured using an Olympus Fluoview Confocal Microscope. Rabbit mAb IgG XP isotype (Cell Signaling Technol.) was used as negative control.
Invasion and migration assay
Cell invasion and migration assays were performed using cell culture inserts (Millipore) coated with or without basement membrane matrix (BD Bioscience, Bedford, MA, USA), respectively. Assays were performed as previously described[16, 17]. In brief, approximate 1 × 105 cells resuspended in 200 μl non-serum culture medium were placed triplicatedly in upper chamber of insert and medium with 10% FBS was used as chemo-attractant in lower chamber; inserts were incubated at 37°C for 48 hours in a 5% CO2 humidified incubator. Cells attached on the inner side of the chamber were then cleared softly with cotton swab and cells outside the insert were stained in 1% crystal violet for 30 minutes. Cells in five random fields were counted under microscope and the relative invasion and migration capacity were interpreted as the average number of cells ± SD per field.
RNA extraction and RT-PCR
Total RNAs from cell lines and tissues were extracted using Trizol reagent (Invitrogen) according to the manufacturer's instruction. Reverse transcription of RNAs was performed using GoScript™ Reverse Transcriptase System (Promega, Madison, WI, USA) as per protocol. The mRNA level of YAP (forward: 5′- TAGCCC TGCGT AGCCA GTTA -3′; reverse: 5′- TCATGC TTAGT CCACTGTCTGT -3′) in cell lines was assessed by semi-qRT-PCR using Taq PCR MasterMix (Tiangen, Beijing, China). GAPDH (forward: 5′-GGAGC GAGAT CCCTC CAAAAT-3′; reverse: 5′- GGCTG TTGTC ATACT TCTCA TGG-3′) was used as an internal loading control. The expression of E2A (forward: 5′- CCACTT CACTG AGTCGC ACAG -3′; reverse: 5′- GTCTCT CCCGAA GGAGG CATA -3′) and YAP in tumor tissues were detected by real-time qRT-PCR using SYBR Green PCR Master Mix (Invitrogen) on the Applied Biosystems 7900HT sequence detection system with GAPDH as endogenous control.
Transient transfection
Short hairpin RNA (shRNA) against human YAP and shRNA negative control (shNC) were bought from GenePharma (Shanghai, China). Plasmids pEZ-M29-E12 and pEZ-M29-E47, encoding fusion protein of eGFP-E12 or eGFP-E47 respectively, were purchased from Genecopoeia (Rockville, MD, USA). Cells were seeded in 6-well culture plates one day before transient transfection, which was performed with lipofectamine 2000 (Invitrogen), according to the instruction of manufacturer. Forty-eight hours after transfection, cells were harvested and the protein levels of the targeted genes were assessed by immunoblot, with GAPDH as loading control.
Lentiviral transfection for stable expression clones
Plasmids pL/shRNA/F-shR with shE2A or shNC (negative control), namely LV-shE2A and LV-shNC, were purchased from Novobio (Shanghai, China). Lentivirus transfection was performed according to the manufacturer's instruction to establish shE2A-expressing stable clones in SW480 cells (SW480/shE2A). The control clone (SW480/shNC) was constructed similarly. E2A protein expressions of abovementioned clones were examined by immunoblot using GAPDH as loading control.
Statistical analysis
Two-tailed Student's t- test, Spearman's correlation or one-way ANOVA were used for statistical analysis when appropriate. All statistical analyses were performed using the SPSS 16.0 (SPSS Inc., Chicago, IL, USA). A two-tailed value of p < 0.05 was considered statistically significant.
Expression of E2A was decreased in metastatic CRCs
Relationship between E2A expression level and clinicopathologic variables in CRC patients
E2A expression
P value
Age (years)
Tumor histology
Tumor site
Rectum and sigmoid
Right colon
Left colon
TNM stage
To further confirm that E2A was also down-regulated at protein level in tumors with metastases, immunoblot was performed using 6 metastatic and 6 non-metastatic tumors chosen randomly from each group. As demonstrated in Figure 1B, metastatic tumors showed lower expression level of E2A protein. Taken together, lower E2A expression associates with positive metastatic status in CRCs.
E2A suppressed CRC cells invasion and migration
Then we used another colorectal cancer cell line, Caco-2, to investigate whether E2A exerted its function in a cell line specific manner. Similarly, we constructed two stable clones, Caco-2/shE2A (expressing shE2A) and Caco-2/shNC (expressing negative control) and as observed in SW480 cells, metastasis ability of Caco-2 cells increased upon shE2A transfection and was suppressed by E12 and E47 (Additional file1: Figure S1A & B), suggesting the metastasis suppression effect of E2A was not cell line dependent. Hence, E2A was a metastasis suppressor gene in CRC.
E2A inhibited the EMT program
To further demonstrate the role of E2A in EMT program regulation, we performed immunofluorescence to visualize these EMT markers in transfected SW480 cells. In coincidence with immunoblot results, immunofluorescence showed that E-cadherin was significantly decreased while vimentin and β-catenin were increased in SW480/shE2A cells compared with SW480 and SW480/shNC cells (Figure 3C). Furthermore, both E12 and E47 restored the expression of E-cadherin in SW480/shE2A cells while suppressed vimentin and β-catenin (Figure 3C). Thus, E2A may suppress invasion and migration through inhibiting EMT in CRC.
YAP was a downstream target through which E2A suppressed metastasis
Next, we asked whether the increased YAP in SW480/shE2A cells led to the enhanced cell aggressiveness. To this end, transient transfection of shYAP was performed in SW480/shE2A cells to down-regulate YAP (Additional file3: Figure S3). As shown in Figure 4C, compared to cells transfected with negative control or blank cells, the invasion and migration ability of SW480/shE2A cells was significantly reduced by shYAP to the similar levels as observed in SW480/shNC cells. This finding suggested that the enhanced YAP by shE2A in SW480 cells was critical in the regulation of cell invasion and migration. Besides, downregulation of YAP impaired invasion and migration capacity of SW480 cells (Figure 4D). More importantly, MMP-9 expression was reduced to 50% of its normal level after shYAP transfection and changes of EMT markers, i.e. increased expression of E-cadherin and decreased vimentin, suggested a suppression of this program (Figure 4E). Immunoblot and immunofluorescence confirmed the expression alterations of E-cadherin and vimentin after shYAP transfection in SW480 cells (Figure 4E & F). Conclusively, YAP was a target through which E2A regulated EMT program to suppress invasion and migration in CRC cells.
Colorectal carcinogenesis is a multistep process mediated by complex cascades of molecular events governing genomic stability and cell proliferation[21]. Distant metastases, rather than the primary tumors from which these lesions arise, are responsible for >90% of carcinoma-associated mortality[22]. In the present study, we demonstrated the suppressive role of E2A in colorectal cancer cell invasion and migration; furthermore, YAP was demonstrated to be a downstream target of E2A in the metastasis of CRC cells.
E2A has been well described as a regulator of early B cell development, and it was dysregulated in lymphoma and breast cancer[13, 23]. Decreased expression of E2A has been reported in metastatic pancreatic cancer cell lines[24]. In colorectal adenocarcinomas, ectopic expression of E47 results in proliferation inhibition[15]. The expression of E2A in CRCs is unknown and its role in CRC metastasis is also elusive. In this study, for the first time we investigated the association between E2A expression and CRC metastasis status and we found E2A was decreased in CRCs with metastases both at mRNA and protein levels, indicating its negative relation to CRC progression. In vitro invasion and migration assays also supported this: downregulation of E2A enhanced metastatic capacity of CRC cells while E12 and E47 could offset this effect. Taken together, E2A has a metastasis suppressive role in CRC. Additionally we found E2A may exert its action by regulating EMT. The EMT program plays an important role in tumor progression and metastasis[18, 25]. Loss of epithelial traits and gain of mesenchymal features make epithelial tumor cells undergo morphological changes and acquire enhanced metastatic abilities. In our study, we found E2A downregulation inhibited the expression of epithelial marker E-cadherin and increased mesenchymal markers vimentin and β-catenin in SW480 cells, indicating EMT suppression by E2A. Considering that E-cadherin was regulated by multiple signal pathways[26], we speculate enhanced β-catenin expression was the main reason for decreased E-cadherin. However, the definite role of E2A in EMT regulation remains further study.
In further investigating the mechanism of action of E2A, we found YAP was regulated as a downstream target. The YAP gene is located on chromosome 11q22, a region which has been described in previous studies to be amplified in several types of cancers[27, 28]. As one of the highly conserved components in mammals, YAP has been proved to be a nuclear effector of the Hippo pathway and was initially identified by mosaic screens in Drosophila melanogaster as a vital growth regulator of cell proliferation and apoptosis[29–31]. YAP is also a transcriptional modulator which has been implicated in stem cell differentiation, control of organ size, and tumor growth[32, 33]. colonic adenocarcinoma tissues show up-regulated YAP expression compared with normal colon tissues, and inducible transgenic expression of a stabilized YAP mutant (S127A) in mice induced colonic adenomas[34, 35]. Indeed, Wang et al. found that YAP was a prognostic marker of CRC and down-regulation of YAP reduced the metastatic ability of CRC cells[36]. In our study, we found YAP was inversely associated with E2A in CRC tissues. This further led us to discover that YAP was a downstream target of E2A as its expression was increased upon shE2A transfection while E12 and E47 transfection could reduce it to normal level. Moreover, β-catenin, which was regulated by E2A, could enhance YAP expression by directly binding to YAP gene in CRC cells[37]. In the present study, we found YAP exerted its function of enhancing metastasis by inducing EMT in CRC cells, which was in consistent with the work of Wang et al.[36]. Importantly, knockdown of YAP in shE2A treated SW480 cells could abolish the elevated cell invasion and migration caused by shE2A. This finding suggested the role of YAP in the E2A regulated inhibition of cell invasion and migration. Hence, YAP plays as a downstream in mediating E2A's function as a tumor suppressive gene in CRC.
The findings of our study suggest that E2A expression is associated with CRC metastasis. By targeting YAP, E2A inhibits EMT program and suppresses invasion and migration in CRC cells. Although E2A's function in cancer has not been fully understood, our findings provide new molecular target and mechanism of action of E2A in CRC metastasis. Therefore, E2A has the potential value to be developed as a new target for CRC prevention and therapy.
This study was supported by grants from National Natural Science Foundation of China (NSFC, Grant No. 30873000, 81300290 and 81372187).
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"But now has He obtained a much more excellent ministry, by how much also He is the mediator of a better covenant, which was established upon better promises."
The promise of God, as revealed unto His servants, is not a compilation of many divers promises of which the sum is the fullness of the intent of God. The promise of God is as singular as He is for it contains all things perfectly joined together as one. When He spoke to Abram He continued to reveal His promise unto him in the manner of the representation like unto two rivers. Thusly He spoke of a multitude of people who would be as the 'dust of the earth' and another 'seed' which would be as the 'stars of heaven'. The first is the natural representation of the descendants of Abram which includes the sons of Hagar, Sarah and Keturah, which seed is indeed fruitful and has filled the earth. Herein is the natural type set forth that God is not a respecter of the pedigree or conduct of the people of His creation for unto every nation kindred tribe and tongue is the grace of God manifested and the glory of God declared. (Matt. 5:45 & Ps. 19:1).
He also told His servant that his seed would be as the stars of heaven but this seed would be of Sarah for in Isaac shall his seed be 'called out' (Gen. 22:12). This did not mean to infer that only the sons of Isaac are to be called children of promise for of Isaac is Esau. Nor is it to infer that of Jacob are the faithful for Jacob has no right of inheritance being not first-born but rather the blessing is in the faithful seed; first of every people of the world and then according to the types and foreshadows of the a specific 'chosen' people. The first stands in ignorant condemnation of Adam unto faith and obedience while the second stands in the type of the condemnation of the law, the ministration of death, unto faith and obedience; "therefore we conclude that a man is justified by faith without the law". These two representations are one promise of the mystery of the gospel, the power of God unto deliverance.
The promise was further defined to Abram in the proclamation of the fact that his people would dwell in a strange land and be afflicted many years (Gen. 15:13). The testimony of the time of history indicates that at the end of this servitude, the children of Israel were delivered on the 'selfsame day' as God had promised (Ex. 12:51) but the promise given to Abram was still in the future. God told Abram that this servitude would be completed in the 'fourth generation' when 'the iniquity of the Amorites was full'. The deliverance from this 'square pile' or 'four-squared dwelling place' would not come to pass until the 'guilt of the sayers' (iniquity of the Amorites) was 'complete', 'perfected', finished' or 'at peace' (full). It was imperative, by the decree of God, that this 'depravity', 'perverseness', 'bent', 'distorted' and 'twisted' nature of this particular people was to have an essential place in His creation, perform a particular function and continue until such a time when it had finished all that was assigned unto it and so the promise unto Abram and his seed would not be completely manifested until 'the iniquity of the Amorites was full'.
"...also the Amorites that dwelt in Chazezon-tamar" (Gen. 14:7)
The land where these 'sayers' dwell is described as a place where the 'archers attempt to divide the palm of date tree'. The fruit bearing 'date tree' or the shade of the 'palm tree' are both descriptions of the Beloved Bride of the King of peace (Song 7:7f). This would seem to indicate that there are outspoken or braggadocios people who are attempting to strike and divide asunder the Bride by the shooting of arrows from a bow. Since the word which is translated in the scriptures, 'sin', is the description of this very act, shooting an arrow and continually missing the mark, then the Word of God has stated to the exalted father of the Faithful (Abraham) that the perfect manifestation of the promise of God to his Beloved people will not be witnessed until the perfect conclusion of the perverse shooting of the arrows. This conduct, which is the constant attempt to divide asunder, is the type of the constant warfare between the flesh of Adam and the Spirit of God.
This promise was confirmed by the mouth of Yehovah to His servant Moses when He commanded him to go to Egypt. Here, after a 'four squared period of time' (forty years) in which he was properly prepared for the labour which was before him, Yehovah God told him that he was to deliver the chosen people of God from the wrath and anger of the 'two-fold siege' of the 'great house' (Egypt, Pharaoh). God had surely seen the affliction of His people and was come down to deliver them; "and to bring them up out of the land unto a good and large land, unto a land flowing with milk and honey; unto the place of the Canaanites, the Hittites, the Amorites and the Perizzites and the Hivites and the Jebusites" (Ex. 3:8). Even though the mighty hand of God had ripped His people out of the grasp of Pharaoh, the physical entering into the land whereon Abram dwelt was yet forty years in the future "till all the people, men of war, which came out of Egypt, were consumed, because that they did not obey the voice of Yehovah; unto whom Yehovah swore He would not shew them the land, which Yehovah swore unto their father's that he would give us, a land flowing with milk and honey" (Josh. 6:6). And when the fullness of time was come and the people crossed the river to inhabit the land of Promise, yet were the Amorites dwelling there. The iniquity of the Amorites was not yet full even though Israel had been delivered from Egypt.
"And I said unto you, I am Yehovah your God: do not fear the gods of the Amorites in whose land you dwell: but you have not obeyed My voice" (Judg. 6:10).
The power of God by the hand of His servant Joshua had defeated 'the sweeping warrior' (Sihon) the king of the Amorites. The testimony of this victory, wherein the mighty hand of Yehovah God was made openly manifest before the nations of the world and the people of Israel, preceded them in their march to the Land of Promise. God gave victory after victory over many nations stronger than Israel as He continued to shew forth His power, His sovereign rule over all things and His love for His people. These mighty displays of the fullness of the Godhead to the natural eyes of man could not bring about faith in Him or His Word and though He never ceased to make these open manifestations yet the people did not believe "but rebelled against the commandment of Yehovah your God and murmured in your tents and said, Because Yehovah hated us He has brought us forth out of the land of Egypt to deliver us into the hand of the Amorites to destroy us" (Deut. 1:27).
The warfare with the speakers of the logic of man according to the passions of Adam in the lusts of the flesh and the pride of his heart is the carnality of the sin that dwells in the flesh of every child of grace. The type as represented in the testimony brings the judgment (Dan) into "captivity to the law of sin which is in my members" (Rms. 7:23). These 'sayers' dwell in the exalted shinning of the field of the deer (mount Heres in Aijalon) which is in the burrowed out holes of the den of the fox (Judg. 1:35). They feign the glory of the hart, which is the Beloved who dwells in the cleft of the rock (Bethar - Song 2:17) yet they are a deceitful thief and cunning predator. They live in burrowed holes into the ground and are likened to the graves of man, wherein dwells the conclusion of corruption, death and decay.
The coast or borders of where the Amorites dwell is 'Akrabbim' or the 'ascension of scorpions'. They dwell like the scorpion from under the rocks (36) and were a people who "had tails like the scorpion and there were stings in their tails and their power to hurt men five months" (Rev. 9:10). They are the wickedness of man whose "mouth is full of cursing and deceit and fraud; under his tongue is mischief and vanity" (Ps. 10:7) and the sin of all men in Adam "whose mouth is full of cursing and bitterness" (Rms. 3:14).
These have been put to tribute for the house of Joseph, the savior of Israel, has prevailed over them yet they have not been utterly destroyed. Therefore "the children of Israel dwelt among the Canaanites, Hittites, Amorites, Perizzites, Hivites and Jebusites" all the days of their habitation in the natural representation of the inheritance which God gave to them. He did not let one word of His promise fail when the King of Peace began to reign over the land of Israel for all things had been fulfilled in type and the Amorites were servants to the king of Israel. All the enemies were subdued under the feet of the Beloved and the King of Peace built the Temple of Yehovah (I Kings 8:56). The promise was completed as God told David that he should not want for a man to sit upon his throne yet God also promised that his kingdom would be established forever (II Sam. 7:12) but the fulfillment of His promise was not yet seen.
Again the natural representation could not show forth the eternal reality of the promise of God. The promise stood in types as did the law but as the handwriting of ordinances could never make the doer thereof perfect so the promise which stands in type and the tangible manifestation cannot make the recipient perfect. The things of this yoke which neither the fathers nor the children can bear are of the earth and dwell in the land with the people of the land, those children formed from the dust of the earth. The promise of God is His Kingdom which is not of this world, wherein flesh and blood have no inheritance.
"For the law made nothing perfect but the bringing in of a better hope by which we draw nigh unto God" (Heb. 7:19).
The hope that is found in the law is in the constant daily performance and perfect obedience to every jot and tittle of the law. Offense in one point is the violation and guilt of the whole and since Adam was formed incapable of being obedient, the scripture has concluded all under sin and God has concluded all in unbelief. God has 'shut up all in unbelief' that he might have mercy on whom He will have mercy (Rms. 11:32) and the testimony of the "scriptures has concluded all under sin that the promise by faith of Jesus Christ might be given to them that believe; but before faith came we were all kept under the law, shut up unto faith which should hereafter be revealed." (Gal. 3:22).
Since the types and foreshadows of the first covenant were made by the ministration of the hand-writings and ordinances of the ceremonies of man, which stand in various washings, the blood of bulls and goats and the observance of days and they cannot purge the conscience of sin, there is need of the bringing in of a better testament which does. This better covenant is by a more excellent ministry than the ministration of condemnation and death. Accordingly the new covenant, which God has made with His Beloved, is unto eternal life in a better Tabernacle, that is to say, not of this dwelling. It is by a 'better resurrection' than that of the natural body "for we know that if our earthly house of this tabernacle were dissolved, we have a building of God, an house not made with hands, eternal in the heavens" (II Cor. 5:1). It is unto a better country "that is, an heavenly: wherefore God is not ashamed to be called their God for He has prepared for them a city" (Heb. 11:16).
The first Adam was formed from the dust and, because he is dust, unto the dust he shall return; the first inheritance is of the earth, earthly. The second Adam, which is the Anointed Salvation of Yehovah, was raised up again, not seeing corruption, and His seed is risen up in Him, who is the first resurrection. They shall never die for they have eternal life and upon them the second death has no power. He is seated on the right hand (place of authority) of the Father and all His children, being raised up together in Him, are seated in the heavenlies in Him (Eph. 2:6). Of the increase of the government of His Kingdom there shall be no end, for it is an everlasting Kingdom, and it is the good pleasure of the Father to give the Kingdom unto His children. They are born of God and are His children and being of the generation of Jesus Christ, the first born of many brethren, they have the right of the inheritance, which is God Himself (Rms. 8:17).
The natural representations stand as the figure of the heavenly reality. The type of the inheritance of the promise of God was unto the natural nation of Israel, a land of abundance and splendour. It was a land of fatness and substance which was appealing to the natural eye and sustaining to the earthen vessels of man; it was "the glory of all the lands" (Num. 13:27). Yet the testimony of God to the natural man of all the beauty and glory of this land could not persuade the natural man to trust God for all men have not faith. The carnal man cannot see the wonders of the works which God has made from the beginning and therefore he is drawn, like the moth to the flame, after that which he can see, feel, hear, touch, taste and smell. These things will their fellow man speak of with 'great swelling words' as they are given by the decree of God to 'soothe the itching ears of man' with the vain philosophies and theology of man. This is the sum total of the beast, which is number of man, "And there was given unto him a mouth speaking great things and blasphemies and power was given to him to continue forty-two months" (Rev. 13:5). The instinctive nature of man is back to Egypt, back to the weak and beggarly elements of the works of the flesh, in attempt to shew forth his worthiness and righteousness before God; This is the warfare of the law in the members against the law of the mind of the spiritual inward man, who is renewed daily.
"It was therefore necessary that the pattern of things in heavens should be purified with these but the heavenly things themselves with better sacrifices that these" (Heb. 9:23).
The Lamb stood as He had been slain from the foundation of the world as a testimony to the work of God being finished from the beginning. He did not need a building of wood, hay and stubble to shed His precious blood for the sin of His people. He entered into a sanctuary not of this world where He poured out His blood upon an altar which man has no right unto. He was bruised for the transgressions and stricken for the sin of His people before Adam was created, therefore He is the author of eternal salvation when as yet time was not yet manifested. His blood not only cleansed His people from their sin but it covered them (atonement) and protected them from the wrath of God which is "revealed from heaven against all unrighteousness of man, who hold the truth in unrighteousness" (Rms. 1:18). It has sealed His people with the identification of being the redeemed of Yehovah and preserves them in Christ Jesus (Jude 1). This the blood of bulls and goats could never do by reason of the imperfection of the creature before the perfect Holiness of the Creator.
The sacrifice for the sins of His people was finished and His people sprinkled in consecration unto the work whereunto they were created in Christ Jesus when God finished His work in the seventh day and He rested. This was manifested in the last days "when in the fullness of time, God sent forth His Son, born of a woman and made under the law, to redeem them that we might receive the adoption of sons; and because you are sons, God has sent forth the Spirit of His Son into your hearts, crying, Father, Father" (Gal. 4:4). Here God shewed forth His promise again "by a new and living way, which He has consecrated for us through the veil, that is to say, His flesh" (Heb. 10:20). This is not unto the laying again of the principles of the doctrine of the Anointed but is unto the newness of life in Him. It is the hope which cannot be seen by the eyes of Adam or polluted by the touch of his leprous hands. The truth may not be tainted by the unruly tongue of those who speak with half the tongue of Ashdod and His people cannot be separated from His love by the rebellion and guilt of the body of this death.
The righteous judgment of the Law of God was completely satisfied by the perfect obedience of the Lamb of God and "by this therefore shall all the iniquity of Jacob be purged and this is all the fruit to take away his sin; when He makes all the stones of the altar as chalkstones that are beaten asunder the groves and the images shall not stand" (Is. 27:9).
"Nevertheless we, according to His promise, look for a new heaven and a new earth, wherein dwelleth righteousness" (II Pt. 3;13)
The children of promise have been made prisoners of hope while they are partakers of flesh and blood. The evidence of the Promise of God is still manifested in the natural world that is seen and the world to come which is unseen. The captivity of this great house in which the children of the Israel of our God dwell and groan, waiting to be delivered, shall continue until the guilt of the Amorites is finished. The accuser of the brethren has been cast out from before the throne of God as 'he who is like God' (Michael- Jude 9) and the 'salvation of Yehovah' (Joshua) has rebuked him saying, "Yehovah rebuke you, Satan, even Yehovah that has chosen Jerusalem rebuke you: is this not a brand plucked out of the fire?" (Zech. 3:2). This was manifested when after forty days wherein "He did eat nothing and when they were ended, He afterward hungered" the Devil came and tempted Jesus. The One in whom the fullness of the Godhead dwelt bodily did rebuke the Devil for His Bride and His finished work; "and when the devil had ended all the temptation, he departed from Him for a season" (Luke 4:3ff). This dragon, that piercing serpent, is the god of this world and the spirit that now works in the children of disobedience. He has a dominion assigned him of the Lord God Omnipotent and he is bound by the limitations placed on his proud waves. He is the servant of Yehovah God and he knows his time is short and though he be the king of the Amorites yet he has been overcome by the blood of the Lamb.
The Amorites however, still dwell in the land with the children of Israel. The Adamic nature still brings arguments, accusations and justifications before the Lord and Maker of all things in spiritual wickedness in high places. He is exalted by the pride of his heart wherein he thinks himself to be God and he continues to speak of things in demonstration of his ignorance. "This know also, that in the last days perilous times shall come: for men shall be lovers of their own selves, covetous boasters, proud blasphemers, disobedient to parents, unthankful and unholy, without natural affection, truce breakers, false accusers, incontinent, fierce, despisers of those that are good, traitors, heady, high-minded, lovers of pleasures more than lovers of God, having a form of godliness but denying the truth thereof: from such turn away" (II Tim. 3:1ff)
Adam speculates upon the ways of God and constantly attempts to explain His doings after the logic and mannerisms of the flesh. He 'balks' at the idea of absolute sovereignty, speculates about eternity and contrives ways and means whereby the Almighty God is to answer his beckoned call. This is the Amorite in the nature of Adam which wars against the Spirit within and when the iniquity of this Amorite is perfected, completed and finished, then shall come the adoption, the manifestation of the sons of God.
"Having predestinated us unto the adoption of children by Jesus Christ to Himself, according to the good pleasure of His will" (Eph. 1:5).
The intricate nature of the manifestation of the Promise of God, as given in the examples left the children in the Holy writ, proclaim His sovereign rule over all things. Every thought, word and deed is set forth in the deliverance of Jacob into Egypt, the abundant prosperity, fruitfulness and captivity were all meticulously set forth in the promise which God gave Abram. The minutest of details carefully and strategically set in proper order and enacted to bring to pass His perfect will at the appointed hour. Each and every thread of the tapestry placed, every man in his order, and interwoven into the fabric of the Will of God as demonstrated in His creation. This is the example of those things which were afore written for the learning of the children of the kingdom during the time of their habitation; "that we through patience and comfort of the scriptures might have hope" (Rms. 15:4). Abram waited an hundred years for his promised heir, Israel waited four hundred years for deliverance "and these all, having obtained a good report through faith, received not the promise, God having some better thing for us, that they without us should not be made perfect" (Heb 12:40).
He is immutable and so His word and His promise, for they are one. He has promised that His people will suffer affliction, heartache, bondage and fear for He has subjected them to the vanity of this world. This subjection is unto hope and hope does not make His people ashamed. Let therefore patience have her perfect work, that you may be perfect and entire wanting nothing (Jms.1:4).
"It is good that a man should both hope and quietly wait for the salvation of Yehovah" (Lam. 3:26).
Your servant in Christ,
(Elder) Chet Dirkes
September 27, 2015

subset: commoncrawl


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Download Videos from Hotstar:- Hotstar is one of the most popular apps which is downloaded on the PlayStore in India. The Star India develops this app mainly for watching the cricket matches, but it was gradually increasing its fame. Based on its popularity later Star India extends this service to Movies, TV Serials, and Reality Shows. Now, This app is the most rated one which will allow streaming the television content on both mobile and PC with free of cost.
Hotstar is available in the mass following platforms like Android and IOS. So, no need to worry at all to watch the best shows which are telecasting on the televisions. As like the Premium apps like Sun next, Voot and Spull. It has the premium subscriptions too. Some of the prominent content is only available to the Premium Subscription users, but free users cannot get that. Meanwhile, you can download the videos offline irrespective with your plan either it is free or premium that doesn't matter.
Hotstar itself providing the offline access to all customers but you can only download certain videos. So that means this is not allowed to download the all the videos which are available to stream. If the video, which you like most was preserved under this category. Now, what you do. There is not much struggle needed to download those videos. Those can be downloaded via third party apps. We will help you to download videos from Hotstar with different Methods.
- 1 Download Videos From Hotstar
Download Videos From Hotstar
From this article, you can download videos from Hotstar on Android mobile and PC. Also included the official process but it has some limits which are already discussed in the above paragraphs. So, better to stick with unofficial methods to get a better result in both android and windows. These are the recommended methods to Download Videos From the Hotstar.
The advantage of the official method is there is no need of any extra app or process to be performed. Even you can download the videos on the IOS OS. This is easy method compare to all because of you can directly save the video in offline. But there are some limits. So you won't get the download link of every video or movie on Hotstar. The Video which is allowed by the Hotstar team. So, it is available to save offline.
Method1:- Download Hotstar Videos directly to the Android and IOS
- Install Hotstar app from PlayStore
- Make sure you have working internet connection (Recommended to connect to WI-FI)
- Open the app. you will see the various categories in the apps home.
- Select the category or search the video using the search button or scrolling the library.
- After tapping on the video, you can notice that the Download symbol in below the video.
- Tap on download Button then you will be asked to select the resolution of the video.
- Now, Choose the any of the resolutions. It will start downloading in the background. You will get a notification once it is done. That's it.
Method2:- Download Videos Using Videoder Android App
Videoder is one of the best apps to download not only the hot star videos also it will download the youtube videos in 4k and 8k resolution. It can be used for downloading videos from Facebook, Vimeo, Voot, Dailymotion and much more. Best third party app to do these things.
- Download Videoder Apk from Here (It is an out sourced app so that you cannot download via play store)
- When you try to install the app, you will get a popup which says you need to enable the Unknown Sources in the Settings.
- Now, Enable the Unknown Sources in the settings app.
- Install the Videoder app from the SD Card,
- Open up the Videoder app and as you can see that the Hotstar app icon on the Home page.
- Click on Hot Star icon. It will load the official Website Home page.
- Now choose the video which you want to download.
- Tap on the Red color Download button from the bottom of the screen. It will ask you the resolution of the video to download. Select the resolution and click on start button.
- The downloading process starts in the background. If you want to see the progress, then click on Download Symbol in the top right corner of the app.
Note:- The Videoder app could not download the video which contains the Premium tag.
Method3:- Download Using Browser
Using browser is the one of the best options for the Windows users, MAC users, Android and IOS users. This method not based on Operating system or Device. Just you need a browser to follow this process. Because it's just a website that can be visited any of the devices.
- Open any browser on your PC or Mobile.
- Visit the website. Here is the Link
- Open Hotstar website from here.
- Choose any one of the videos.
- Copy the Video URL which you want to download.
- Paste the URL in the Above website. then it will show you some resolutions of the video. click on it.
Method4:- Download Videos & Movies from Hotstar on Windows 10, 8, 8.1, 7 using Command Prompt
This is not a recommended method, but this is also the way to download videos from Hotstar. This requires some technical knowledge to perform. So, better to stay with browser process. And also a complicated process which needs an additional file. But there is a major advantage by using this process. You can download the videos which are having a Premium tag.
- At first download the Zip file from above.
- Extract the zip file using Winrar or related software.
- After Extracting the Zip file and store in the specific location. Then open the folder.
- Click on the hotstarlivestreamer.bat file. It will open the Command window.
- Now, paste the link (URL) of the video. Press Enter.
- It will show you available Streams like 234p 360p 404p 720p 1080p. Now, type the (quality) pixels EG:- 720p.
- Type d for downloading the video or p for streaming the video. Go with d because you aim to download videos from Hotstar.
- You will find the downloaded video in the Extracted Folder/Videos.
Final Words:-
I think this is not a big matter to download Hotstar videos with android mobile. Using browser is the best method in my case. Even you can download premium tag videos with that one. I hope you like this article.
Share this article with your friends and help us to grow. I will meet you in the next one.


text:
No Recent Price Change
4377 Stoney Poynt Court
North Charleston, SC 29405
- Residential
- Single Family Detached
- Sold
Agent Remarks
4377 Stoney Poynt Ct is a must see! This is a custom-built waterfront home with a dock on TRUE deep water & a salt water pool. Located on a quiet cul-de-sac in a gated community this home is just a short distance to I526 and 20 min. to downtown Charleston and the beach. At just over 3500 sq ft this home features panoramic views of the Ashley River, deep water dock with a 16,000 Lb. boat lift, Oak hardwood floors throughout, Large master suite on the first floor with access to the screened porch, spacious living room with custom built-ins, the kitchen opens to a second family room & a formal dining room. Upstairs you will find 3 additional bedrooms and two full bathrooms. This one owner home has a new roof, new HVAC system and new windows throughout. Please click ''more'' to read more info. When you enter the home you are immediately drawn to the panoramic views of the Ashley River. The living area is spacious with French doors leading to a huge screen porch that overlooks the salt water pool and dock. A gas fireplace and built-in bookshelves highlight the room. Looking up at the vaulted ceilings you are impressed by the trim detail in the living area and formal dining room which continues through the home. The spacious kitchen features granite countertop, custom cabinets, microwave/oven wall combo, glass cooktop, wet bar for entertaining plus a large walk in pantry. The kitchen opens to an additional family room that is flooded with natural light making it a great space for a home office. Gorgeous oak hardwood floors are throughout the entire downstairs including the master bedroom. The master bedroom is downstairs with a beautiful bathroom featuring a walk-in shower with frameless glass door, large jetted tub and His & Her closets are a work of art – with custom cabinets and built-ins. The laundry room on the first floor has plenty of cabinets along with a granite countertop (the laundry sink was removed but the plumbing is still there and can be installed easily). Upstairs you will find a spacious second master with a full bathroom, large walk-in closet and additional storage. There are two bedrooms on the opposite side of the hall that share a full bathroom. The 3 car garage features 5 parking spaces, custom workbenches with cabinetry, and plenty of storage for tools and boating equipment. There is also an elevator shaft roughed in from the garage up to the third floor. The location of this home on the Ashley River is amazing. PLEASE MAKE SURE YOU VIEW OUR FLOORPLAN ONLINE AND VISUAL TOUR!
Sale Info
Listing Date: 07-20-2017
Sold Date: 10-20-2017
Aprox Days on Market:
2 month, 29 days ago
Listing Sold:
1 Year, 6 month, 2 days ago
Baths Info
Halfbaths: 1
Fullbaths: 3
Building Info
Year Built: 2006
Floors: Ceramic Tile, Wall To Wall Carpet, Wood
Foundation: Elv. Construction
Exterior Features
Misc Ext: Boatlift, Dock - Existing, Elevator Shaft, Dock - Floating, Porch - Full Front, Gutters, Fence - Iron, Lawn Irrigation, Pool - In Ground, Porch - Screened
Interior Features
Msc Intr: Ceiling - Cathedral/vaulted, Elevator Shaft, Garden Tub/shower, Kitchen Island, Ceiling - Smooth, Walk-in Closets, Wet Bar
Lot Info
Acres: 1
Lot Desc: Cul-de-sac, Waterfront - Deep, .5 - 1 Acre
Parking Info
Gar Pkg: Attached, 5 Car Garage
Property Features
Amenity: Dock Facilities, Gated Comm
Desclocation: Family Room, Gas Logs, One
Green: Tankless Water Heater
Roof: Architectural
Style: Traditional
Stories: 2 Stories
Special: Flood Insurance
Rooms Info
Beds: 4
Ground Level: No
Rooms: Eat-in-kitchen, Family, Laundry, Pantry, Separate Dining, Sun
SqFt Info
Sqft: 3,541
Utilities / HVAC
Cooling: Central
Heat: Electric
Water Sewer: Public Sewer, Public Water
Elem: Meeting Street Elementary at Brentwood
Middle: Zucker
High: North Charleston
Courtesy of listing office Carolina One Real Estate


text:
Amazing photos!!
I have used a picture taken in our Greenland trip for last week's photo challenge. It actually gave me a chance to rewind the experience of one of our most memorable trips. After browsing through the pictures there, I can't help creating another post for it.
This picture was taken at a helicopter. It was not those kinds of "Greenland by Air" type of tourist excursion, but instead a ride out of necessity. Basically our route started in London. We flew to Reykjavik at Iceland and stayed there for one night. Then in the next day, we flew to Kulusuk, a small town in eastern Greenland which has an airport with a proper runway. Then from Kulusuk, we flew to Tasillaq, our base for this Greenland trip, via a helicopter.
While the ride was just 10 minutes, it wasn't cheap. Anyway as we approach Tasillaq, we were really excited as we've never imagined to be able to go to such an exotic place. Tasillaq isn't a big town but because there is no formal means of public transportation in the town, the only way to navigate around is on foot. The walk isn't easy because there are slopes and the roads are not in good conditions. People living are okay to outsiders although we did not talk too much to the locals.
Living in Tasillaq was an interesting experience. We lived in a small "hotel" which is actually a group of small wooden houses. Supply was very limited – indeed in one morning we did not have bread for breakfast – because the shipment of bread was late. There is no Internet and if the weather is bad, all excursion will be cancelled. I won't say it's a pleasant stay but it definitely is worth a try.
Thank you very much!!Reply
[…] I said in the previous post, Tasiilaq is not a big town and walking is the only means of navigating around different places. […]Reply
Such a beautiful scene, great photo!Reply
Thank you very much!Reply


text:
US-IL-Lisle: Senior SQA Engineer
To see other opportunities,
please visit http://sqa.fyicenter.com
Date: 24-Dec-2012
Senior SQA Engineer
test interfaces and document test results identifying trends and escalating concerns as necessary; recognize deficiencies and coordinate opportunities for standardization between internal developed products.
* Bachelor's degree from an accredited college or university with major course work in computer science, quality assurance, communication, business administration, or a related field required. Equivalent work experience in a similar position may be substituted for educational requirements.
* Minimum five years experience in testing interfaces, quality assurance, or related field required
*Minimum (3-5) years of SQA and system test experience.
*Working knowledge VoIP (Voice Over IP) and related protocols including SIP, SDP, RTP etc..
*Working knowledge of legacy telecom protocols such as ISDN and SS7
*Knowledge of Networking protocols (TCP/IP, DNS)
*Experience with SIP Simulators/tools and Network Protocol Analyzers for troubleshooting Networking and VoIP issues.
*Experience with Unix and Linux Operating Systems
*Experience with Oracle database and SQL queries is a plus
*Experience with Automation tools such as Selenium, or QTP and UNIX shell scripting is a plus
To apply for this position, please visit
this Website.
Thank you,
Team SQAFYI - Software QA Resource FYI


text:
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text:
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If you were arrested for driving while intoxicated, I am ready to use my skills and knowledge as an El Paso DWI attorney to protect your rights. Contact me to schedule a consultation.
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DWI cases run two courses. One is the criminal aspect. The other is the administrative process that involves possible license revocation. We move quickly to challenge and possibly overturn the suspension and identify ways to secure an occupational license to drive for work-related purposes. If you have been charged with DWI, call the Quinonez & Salayandia Law Firm, PLLC to represent you best interests.
Address : 11890 Vista del Sol A-115, El Paso, TX 79936
Phone : (915) 301-8890
Ortega, McGlashan, Hicks & Perez, PLLC
Being charged with driving while intoxicated (DWI) in the state of Texas is a serious offense, oftentimes leading to heavy fines, driver's license suspension, and prison time. If you are in El Paso County and need effective representation from a skilled defense lawyer for a DWI case, Ortega, McGlashan, Hicks & Perez, PLLC is ready to give you the assistance you need. Our attorneys have years of experience practicing law in Texas so we know how the state's legal system works.
Address : 609 Myrtle Suite 101, El Paso, TX 79901
Phone : 915-201-0590


text:
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Documents: 2010 Policy Directives
#10-18-EMP: Engagement Call-In Of Nonexempt Food Stamp Participants
(This Policy Directive Replaces PD #00-85R and PD #02-34-EMP)
Engagement Call-In Of Nonexempt Food Stamp Participants Food Stamp (FS) participants between the ages of 16 and 59 are required to provide information regarding their work registration status.
Participants who are deemed employable (nonexempt) must comply with the FS Program work requirements as a condition of eligibility. The FS Program work requirements for nonexempt participants include participation in a Food Stamp Employment and Training (FSET) program as assigned by the Agency.
Nonexempt FS participants may be required to participate in an FSET program for up to 30 hours per week. The total hours of participation in an employment and training program and any hours of paid employment may not exceed 120 hours per month.
The maximum number of hours of work experience assigned to a participant each month may not exceed the value of the participant's monthly FS benefit divided by the higher of the state or federal minimum hourly wage.
Failure to comply with the FS Program work requirements will cause nonexempt FS participants to be ineligible to receive FS benefits and subject to durational sanctions.
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One of the mysteries of the English language finally explained.
Definition of kilometre in English:
(also km) (US kilometer)
A metric unit of measurement equal to 1,000 metres (approximately 0.62 miles).
- 'This is typically displayed in minutes per mile or minutes per kilometer.'
- 'In the prairies where they live, they can be seen for a kilometer and a half (nearly a mile).'
- 'The event consisted of a five and ten kilometre run and walk as well as a one kilometre for the very young, the old and the not so fit.'
- 'What he calls a crack is more like a canyon, at least 300 metres deep up to a kilometre long yet only a metre wide.'
- 'As with metres and kilometres, we need some new units, or we'd need a lot of zeroes!'
- 'Next we will be having our speedometers changed from miles per hour to kilometres per hour.'
- 'It seems to me like if it takes more kilometers to make a mile, then it should take more kilograms to make a pound.'
- 'The appellant was informed that he had been clocked at 97 kilometers in a 50 kilometer per hour zone.'
- 'It ought to be pointed out that calculating passenger kilometres is an inexact science.'
- 'Families then move a few miles or kilometers away to an area richer in resources.'
- 'Selecting kilometres will give you a countdown in metres - you can't have a mixture of both.'
- 'The scale of alteration can vary in extent from less than a centimetre to many tens of kilometres.'
- 'Easier, but not easy, and still only around a billionth of a millimetre over five million kilometres.'
- 'Islands of ice kilometers long and up to two metres thick are common here.'
- 'It is one kilometre wide by a kilometre and a half long and the chicks are protected from predators by electrified wire fencing.'
- 'Once in a while, the funnel of air drops from the sky - it can be as narrow as a few metres across or as wide as two kilometres.'
- 'A meter is about three feet and three inches and a kilometer equals about six tenths of a mile.'
- 'The hole it would leave would be two kilometres long and one kilometre wide.'
- 'People drive in kilometers, fill their gas tanks by litres and heat their homes in degrees centigrade.'
- 'They will move around about a quarter of a kilometre an hour and if you measure their rate of moving, they have small home ranges.'
There are two possible pronunciations for kilometre: one with the stress on the ki- and the other with the stress on the -lo-. The first is traditionally considered correct, with a stress pattern similar to other units of measurement such as centimetre. The second pronunciation, which originated in US English and is now also very common in British English, is still regarded as incorrect by some people, especially in British English
Late 18th century: from French kilomètre (see kilo-, metre).
Further reading
Top tips for CV writingRead more
In this article we explore how to impress employers with a spot-on CV.


text:
Now that Halloween is behind us, let's talk about something really SCARY!
For investors around the world, the relative calm of the seven-year bull market is over. In the past several months, the markets have transitioned into a what can realistically be called "a new and extended period of volatility." We're not talking about an adjustment period of quarter or two, either. Going forward, volatile is the 'new normal.'
But it's more than the typical business cycle that's behind these new fits and starts. Five decades of outsourcing and capital market integration have resulted in interconnected global markets and supply chains that are highly sensitive to disruptions.
Furthermore, instantaneous information and market reaction as well as rapid and automated computer trading intensify market sensitivity to uncertainty.
There are also several event-driven causes of this new, structural market volatility that are connected either in their causes or by their impacts:
- The absolute and relative decline of American economic and financial influence in the world is leading to less stability in global markets and the international financial system
- The Brexit and the falling value of the pound create uncertainty and volatility in the currency markets around the world
- China has no easy or quick solution for its slowing GDP growth rate, which is down to 1990 levels, trillions in bad debt in its 'shadow economy' and a debt-to-GDP ratio of 3:1.
- The Wealth Gap in the US is now at 1929 levels, signaling record high income inequality and fewer buyers in the market. Thin market volume means higher volatility
- Persistent wage stagnation means anemic consumption and weak economic growth outlook….not good for corporate profits going forward
- The Fed has run out of stimulus options, hinting of a rate hike in December of 2016, even though workforce participation rates remain at mid-1970s recession levels
All of these macro factors tell us we're entering an extended period of uncertainty and market volatility.
For traders, of course, volatility is good news. For investors, however, market gyrations can reduce the healthiest of portfolios to a fraction of their value virtually overnight.
We've seen it twice already in the past 15 years alone.
What should investors do to smooth out portfolio performance (and their anxiety curve)?
Take control of their portfolios by diversifying into tangible, non-correlated assets, of course.
Real estate is a terrific option to accomplish this, as strategic real estate assets in the right locations hold their value better, deliver stable and competitive returns, and let you leverage the strong dollar against local prices.
Real estate is known as the "original resource" and will continue to be relevant any time people need a roof over their heads, a place to go to work, or a venue in which to be entertained.
Be sure to make real estate diversification part of your financial plan, and put yourself in the driver's seat by being exposed to as many deals as possible.


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This will be the only conscience-based post I will put up here. I set up some ideas about how I want you to view this space.
I do believe we should be allowed to express an opinion, the only problem I have is the reason behind it. Oddly enough, the Canadian Charter of Rights and Freedoms will enable people to be racist (for example), though it also protects those who are subjects of hate-speech.
You have every right to criticise a religion, a government or a celebrity; yet, you need to understand others have the right to challenge your process. Reposting a meme or a rant from someone who fits in your hate space is not a form of dialogue. Okay, it is if you are 17 years old and having an argument over the best episode of 'Big Bang Theory'.
Be cautious of what you say, as we are in a place of wanting a verbally cleaner place. This may mean taking stock of what you term as "important" in regards to ethics and morals. There is not an increase in xenophobia, only now we see those committing it no longer being afraid to hide it. That being said, those of us looking for a better place also need to be aware we cannot be arseholes either.
I have tried to combat the hate with love, but that does not work all the time. I do occasionally ask for those in full speed hate to take a day off. It could mean they are tired of watching Bravo Television for six hours a day. Or, they may need to use the facilities.
Sometimes having a poo can make things feel so much better.
Cull your hate-filled herd if you need to, I do it quite often. I also encourage some engagement (this applies to close relationships, mind); this is a reminder to both of you that there are parameters to adhere to. Those mashings that involve unknown combatants should be taken with a bit more care. By care, I mean do not engage. These conflicts are for "likes" more than anything – disregard. Agreements need to be set up, arguments hashed out – hopefully ending in a beverage of choice (if a personal meeting) or a meme hug (if on the Internet).
Easy-peasy, lemon-squeezy.


text:
Narrow Search
|Job Title
|Date Posted
|Engine Electronics Software Engineer
At Volt, we understand that IT specialists are different: you're looking for more than just a job – you're looking for the next step in your career path. We are one of the nation's leading technical recruiters, and we've earned that ranking by digging beyond the keywords on your resume. Our goal is to place IT professionals in positions where both the employee and our client can thrive.
If you are an IT specialist with java programmer, software engineer, QA analyst, IT project manager, web developer, or software developer experience, take a look at Volt's IT jobs – when you find a job that looks like a good fit, apply online. Join our Talent Network to receive notifications for new positions that match your skills – including jobs not posted anywhere else.
With hundreds of positions available, we can help you find a job you love. Our dedicated IT recruiters are ready to work with you to help you achieve your next career goal.
You deserve a job you love. Find yours at Volt.
|Job Title
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|Engine Electronics Software Engineer


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Goodbye Gintoki and friends: Gintama manga to enter final arc this year
It seems that we will be saying goodbye to a lot of beloved characters soon, as Gintama mangaka, Hideaki Sorachi has made an important announcement with the release of the 63rd volume of his Gintama manga, as he announced that his manga will be entering the final arc in 2016.
During last year's Jump Festa event, the mangaka has said that he has been receiving several letters after he announced that he plans to begin the final arc this year. He said he wanted to clarify what he said during that event and confirmed that the final arc will indeed begin within this year.
The comedy manga has been known to break the 4th wall and parody several other anime and mangas, and has gotten a surprisingly large female fan following. The manga has won plenty of awards, and has also received plenty of anime adaptations, as well as an anime film. More details on when the manga will finally end, will be announced in the days ahead.
Don't miss a single update from SGCafe! Follow us on Twitter: @SGCafe
Read more on your favorite anime here on Sgcafe! Also join in on the community's discussions at http://forums.sgcafe.com
Source: ANN


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A Venetian Villa in exclusive
Villa Abbazia, located a short distance from Venice in the inland of Trevignano,
is the ideal place to celebrate a wedding or an authentically romantic honeymoon.
Our staff of highly qualified professionals can assist you in every aspect of such an important occasion.
Upon request, we can provide a specialized consultant.
When making plans to celebrate a special occasion like a wedding or weekend with friends, why not rent your own private villa:
Hotel Villa Abbazia, one of the region's most splendid Venetian villas.
Whatever the occasion to celebrate, this luxury hotel with the exclusively starred La Corte Restaurant,
the garden, l'Espace Rouge et Noir is conveniently located, near Venice and the airports of Treviso and Venice.
All eigtheen rooms of this exclusive villa, rich in history and charm can be reserved along with an additional twenty rooms
at our more contemporaty sister hotel, Dei Chiostri. At Espace Rouge et Noir host your pre-bridal and stag parties.
Our salas for events and conferences accomodates thirty to sixty people.
These spaces are adapted to host all types of events and are equipped with the latest technology.
The conference room is illuminated in natural light, but during the summer calendar of conventions, conferences, product presentations,
fashion shows and award ceremonies, meetings can held in the grand park with century old trees and a view of the sea.
A weekend between friends and family In the intimacy of the century old Villa Abbazia – rented exclusively for your private use –
you can meet friends from secondary school or university, riunite the entire family to
celebrate graduation or a new job contract, or blow out the candles of an important birthday.
It is the ideal villa to meet convivially with friends and parents.


text: BMW 1 Series Introduced at the 2016 Guangzhou at Auto Show last November, the series 1 BMW Sedan is now on sale in China. The compact luxury sedan starts at CNY 2,04,800 (INR 19,90,104.9). The BMW 1 series is available in three models: 118i, 120i and 125i, and in the ...Read More »


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Phoenix out of top 20 as a best place for first-time home homebuyers
Phoenix, a longtime bastion of low home prices, fell just outside the top 20 cities in the U.S. as a good place for first-time homebuyers. An index created by SmartAsset that ranked cities in terms of mortgage lenders, volatility and average price per square foot among several other factors, found Phoenix ranked No. 21 in the U.S.Topping the list were Pittsburgh, Oklahoma City and Omaha, Nebraska. Pittsburgh received the top nod due to its high percentage of loans funded and low average cost per square foot. The cost per square foot was one of the high markers for Phoenix, which has seen prices steadily rising during the last two years after the Great Recession wiped out much of the home equity in the Valley. While many areas have recovered to pre-recession price levels, others have not. Yet SmartAsset found Phoenix with an average price of $130 per square foot. That equates to roughly $221,000 for a 1,700-square-foot home.


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Gray Puksand & SHAPE help Victoria Legal Aid make bold office moveGray Puksand & SHAPE...23/02/18
SHAPE BOOKS $335 MILLION AS OFFICE MARKET SURGESAustralia's leading fitout and...10/05/16
Innovation you can affordAn environment that fosters...09/05/16
The Department of Education and Training (DET) project had
two stages over three levels, including kitchens, open space
workstation areas, two communications rooms, meeting rooms with
state-of-the-art hearing loop systems, executive offices and a
reception area.
The base building had a 5 Star Green Star rating, which
CSA wanted to match. SHAPE took progression of
the site when the project was in the preliminary design phase,
meaning that not only could we assist with the Green Star
components, but we could also ensure that the design was executed
correctly and the project was able to meet the challenging four
week timeframe.
Client: Department of Education and Training
Floor Area: 3,000 m²
Delivery Model: Lump sum
Location: Brisbane
State: QLD
Project type: Fitout, Sustainability, Government
Completion date: March 2010
Duration: 8 weeks
Architect: PDT Architects
Sector: Office Government
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text:
Mac Nightmare Adventures: The Turning Thorn Game
Two weeks after the events of Nightmare Adventures: The Witch's Prison, Kiera Vale finds herself drawn back into a web of occult sciences, mysterious dark entities, and government secrets -- woven by the agency known as A.R.C.A.N.E.. Kiera is happy to receive the call, but soon after her arrival finds that things are not at all how she remembers them to be. What are these agents hiding, and why didn't they tell her the whole story? What is the true nature of the Blackwater "Witch"? Why is Dr. Thorn's young daughter locked up in a containment cell? From the top secret labs in the depths of A.R.C.A.N.E.'s headquarters to the distant spires of alien worlds, Ms. Vale will seek answers, solve cryptic puzzles, banish evil otherdimensional creatures, and perhaps even make a new friend over a game of Piggy Princess along the way, in Nightmare Adventures: The Turning Thorn.
One hour or 60 minutes free trial demo version of the game is provided above. There are some games with more than 1 hour (80 minutes) time limit. Windows7Games.com provides virus free games setup files which are placed on secure server. Downloading time of game setup files varies depending up on size and your internet connection speed. Before buy your full version legal copy with various available payment methods you can play free demo of downloadable casual game. You have to purchase activation code or serial key for full version of the game by credit card, Visa Card, Master Card, American Express Card, Discover Card or Via PayPal.
Read Nightmare Adventures: The Turning Thorn Game Features
- Get the Strategy Guide!
- Check out our Blog Walkthrough
- Buy full version of this game for PC!
Minimum System Requirements for Playing Game
- Operating System: 10.10/10.9/10.8/10.7
- Processor: 2.0 GHz (INTEL ONLY)
- RAM (Memory): 1024
- Free Space on Hard Disk: 385 MB
More Information About This PC Game
- Game Size: 371.98 MB
- Date of Release:
- Game Category: MAC Puzzle Games


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Orthopaedic Surgeon in Los Angeles, California (CA)
Robert M. Bernstein MD
- Cedars-Sinai Medical Center444 S. San Vicente Blvd., Suite 600Los Angeles, California, 90048 - United StatesPhone: 310.423.5224
About Our Practice
Robert M. Bernstein, MD, Director of Pediatric Orthopedic Surgery at Cedars-Sinai Medical Center. In addition, Dr. Bernstein is a member of the Cedars-Sinai Institute for Spinal Disorders and Cedars-Sinai Orthopedic Center. He is also the orthopedic surgeon for the Skeletal Dysplasia Clinic at Cedars-Sinai.
Board certified in orthopedics, Dr. Bernstein is internationally recognized as an expert in the areas of scoliosis and spinal deformity, hip dysplasia, clubfeet, children's fractures, limb lengthening, children with limb deficiencies, arthrogryposis and other skeletal dysplasias.
Dr. Bernstein's research findings have been published in several peer-reviewed journals, including the Journal of the American Academy of Orthopaedic Surgery, Journal of Pediatric Orthopaedics, Spine, Journal of Orthopedic Trauma, Current Opinion in Orthopaedics and the American Journal of Orthopaedics. In addition, Dr. Bernstein has written a number of chapters for various orthopedic textbooks and has lectured internationally.
Dr. Bernstein is a member of the American Academy of Orthopedic Surgeons, the Pediatric Orthopaedic Society of North America, the Scoliosis Research Society, the Association of Children's Prosthetic and Orthotics Clinics and the Western Orthopaedic Association.
Dr. Bernstein received his medical degree from the University of Southern California School of Medicine. He completed an internship at the University of South Florida and an orthopedic residency at the Harvard Combined Orthopaedic Residency Program at the Massachusetts General Hospital. He also completed a fellowship in pediatric orthopedics at Children's Hospital in Boston and a fellowship in spine surgery at Beth Israel Hospital in Boston.
- Orthopaedic Surgeon
- Board Certified
- Fellowship Trained
- Spinal Deformity
- American Academy of Orthopaedic Surgeons
- Scoliosis Research Society
- Cedars-Sinai Medical Center


text:
I want to be able to Clone a HDD to clone the image onto Crucial BX500 SSD's I've purchased.
I want to do this so this will copy all partitions across and I do not want to install software in Windows to have to later remove. I've got to do this across a lot of individual machines as I'm replacing their existing slow mechanical drives with Crucial SSD's.
Does this Acronis software allow me to build a bootable USB UEFI stick to do this?
If not could someone suggest Free Software so I can achieve this from Curucial or elsewhere.
Acronis does have an option to create a bootable USB drive, but you need to install the software on at least one system. I think if you pay for Acronis, they may allow you to download an image to use on a USB drive. Many of the free cloning options require extra work unless the two drives are the same size.


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A Team of Inspired Personnel
Field engineering support is available to our customers to ensure total dependability.
Our state of the art manufacturing facility is one of the vital driving forces that has enabled us to achieve the position we currently occupy in this competitive industry. We maintain modern manufacturing facility; featuring the latest and technologically most advanced machinery that enables us to achieve cost effective production methods and consistent high performance products. With the help of our facilities, we perform the following tasks, which ensure that the product is effective, efficient and economical.
Being a highly customer-centric organization, we always strive to meet our customer's high priority demands. To make sure that our product matches their requirements, we get their approval before the order is executed for bulk production. This saves cost and time at both the ends.
Depending on the mode of transportation and customer specifications, packaging is done to make sure that the consignment reaches you safely.
To ensure that we are able to fulfill even the most bulky orders at a short notice, we have a spacious warehouse that has the capacity to store large quantities of products.
Our team - Our backbone
Our team of skilled technicians and experts is constantly working towards providing better solutions to our clients. Our research and development team is laboriously working towards coming out with products having superior designs, increased tolerance levels and other improved features so that your production costs are also controlled. We are constantly reviewing all the operational dimensions and reviewing the potential areas for technical breakthrough.


text:
Update order email along with the email associated with the account with a single process
You can update the order email of an order directly from the order details page. You can also update the email address linked to an order by entering the Order Id directly.
No additional configurations required.
Yes, all our modules come with FREE installation support. Just send us an email with your order number and temporary shop access credentials.
Just send us an email with your order number and we will send you the latest version of the module abosultely FREE.
Unless it is a major Magento version upgrade you do not need to purchase the module again. Just send us an email with your order number and we will send you the updated module.
Ensure that you have copied the files in the correct location, they have the right permission. Remember, to do setup:upgrade, setup:di:compile after enabling the module. If the module is enabled and you are still having issues please contact us.
Send us an email with your order number, issue details, steps on how to recreate it and temporary SSH & Admin access to your shop.
The module is Open Source so, nothing stops you from doing so but, we will provide support only for one store.
Dear Schogini, Thank you for 10 years of frontline service. I have continuously been impressed with your fast, expert, and courteous service that has continued to improve and expand, keeping not just up to date but also user friendly. I never feel intimidated or stupid, even though I know the problem is not with Schogini or its services but from my side. Thank you for 10 smooth years, you have made life for me on the Internet possible!
Tom Hibi
Manager, Tom's English Services
One major factor, which pushes customers to choose online shop over physical shop is the possibility of having their order details always available online. They can always login to their account and see their purchase history and in the case of downloadable products, get the updated version. But there are some cases where the customer may have a change in their email address or the item was purchased by a subordinate or client. In that case, they will require a change in the email address linked to an order and the account used for the order.
This Update Order Email Magento module will make this process easy for you. All you need is the Order Id and the new email address and you will be able to update the order's email address in a jiffy. You can also update the order email address from the order view page in the backend using this module. Now you don't have to do the make your hands for such things.
Setup & Configuration:
This module will save you a lot of time and also your customers will always be happy.


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This adventure has moved quickly. I saw the job posting online on July 12 and emailed requesting more info. I spoke with someone the following day and submitted my application letter on July 16. My interview was July 21 and I received the job offer July 24.
I then began the arduous task of obtaining my work visa. I had to get a police background check and then take it and my original college diploma to the Secretary of State's office to get them notarized and have the State Seal attached. This sounds simple, but the instructions I received came in different emails and with the 12+ hour time lag, it was all confusing. In retrospect, I probably should have gotten a duplicate diploma and used that one for the visa, since it gets crimped by the notary. Oh well. And nobody thought to tell me that the passport photo I needed for the visa application would not be accepted if I was wearing earrings!! So just when I thought I was done, I had to go get new photos taken.
I also had to pack up my entire house: two bedrooms and two bathrooms and a finished basement. I gave A LOT away, sold most of the furniture, and packed away things I wanted to keep. I learned that I have too many possessions. Even though I only rented a 5×10 storage unit, having it all piled up like that just shows the amount of STUFF I've accumulated.
Finally, eventually, the paperwork was all submitted to China, but with the delays, it was too close to the beginning of the school year. There wasn't enough time to process the work permit. So, after checking with other people who have taught in China, I agreed to apply for a tourist visa to get me in country. Of course, I will still need the work visa, but it seems that this route has become standard operating procedure.
So, I paid for an expedited tourist visa, which arrived last Friday. After confirming with the agent in Chicago on Thursday that it had indeed been obtained, I purchased a ticket for Friday afternoon. She overnighted the visa to me and six hours after receiving it, I got on the plane!
After staying 24 hours in Los Angeles for orientation with the US recruiter, I boarded a plane for Beijing at 2am Sunday morning.
I arrived in Beijing at 5am Monday after a twelve hour flight.
After going through Customs and Immigration (two separate areas) I finally managed to arrive at my gate – with ten minutes to spare! I ran over to the nearest store to buy some water since I was so hot and sweaty, but my card was declined!
I could have cried.
But instead I went across the hallway to a restaurant and was able to get a juice and a bottle of water. They accepted my card without a hitch, and I able to sit and enjoy the juice before going back to the gate for boarding.
Four hours later, I had arrived at my destination and my new chapter began.