text:
- Research
- Open Access
Identification of stroke-associated-antigens via screening of recombinant proteins from the human expression cDNA library (SEREX)
© Machida et al.; licensee BioMed Central. 2015
- Received: 24 October 2014
- Accepted: 13 January 2015
- Published: 22 February 2015
Because circulating antibodies against a variety of antigens have been detected in patients with coronary heart disease, carotid atherosclerosis and those who have suffered a stroke, it is suspected that immune response may be one of the mechanisms of atherogenesis The objective of this study is to identify novel antibodies in ischemic stroke patients by screening the expressed recombinant proteins using a human cDNA library (SEREX).
To identify the candidate antigens, cDNA library was screened by SEREX using plasma from ten patients with ischemic stroke. Subsequently, via ELISA using recombinant proteins and synthetic peptides, the serum antibody levels were measured in two independent patient/healthy donor (HD) cohorts (142 and 78 in the 2nd screening and a validation cohort, respectively).
The initial screening resulted in the identification of six candidate antigens. Of these antigens, replication protein A2 (RPA2) was determined to be the antigen associated with stroke (P < 0.05) by ELISA with 2nd screening and validation cohort. Multifactorial logistic regression analysis showed that the increased levels of the RPA2 antibodies (RPA2-Abs) associated with stroke independent of other risk factors for stroke (P < 0.05). Receiver operating curve analysis demonstrated that the area under the curve from ELISA using GST fusion RPA2 and synthetic peptides (bRPA2-132) were 0.867 (95% CI: 0.798-0.936) and 0.971 (95% CI: 0.940-1.00), respectively. If the cut-off value of the bRPA2-132-Ab level was determined to be 0.334, the sensitivity and specificity of the antibody level as the diagnostic marker for stroke were 0.323 (95% CI: 0.209-0.453) and 1.00 (95% CI: 0.713-1.00), respectively.
SEREX identified RPA2 as the antigen associated with ischemic stroke and serum auto-antibodies against RPA2 elevates in stroke patients. RPA2-Abs could become a biomarker for the evaluation of ischemic stroke at risk.
- Stroke
- Antibody biomarker
- Atherosclerosis
Current studies recognize the role of inflammation in all stages of atherosclerosis and, subsequently, the immune system has become a subject of focus in this field [1]. Both innate and adoptive immunities, which recognize endogenous antigens such as proteins, carbohydrates, lipids and nucleic acids, influence the progression of atherosclerosis [2]. The proposed antigenic proteins that evoke immune responses and affect this progression include oxidized-low density lipoprotein (oxLDL), phosphorylcholine, heat shock proteins (Hsps), apo-A1 and phospholipids as well as the antibodies for these antigens, all of which have been found in elevated levels in patients diagnosed with a cardiovascular disease [3]. Ischemic stroke is the major cause of worldwide mortality and morbidity and one of the primary mechanisms of stroke is atherosclerosis. Along with other cardiovascular diseases, the increased antibody levels for antigens such as Hsp 27, 65 and 70 have been reported in patients with ischemic stroke [3].
Serological identification of antigens by recombinant cDNA expression cloning (SEREX) is an approach to antigenic protein identification that enables large-scale screening [4]. Although originally developed to screen cancer-associated antigens, this method has also been applied to vascular conditions such as transplant-associated coronary artery disease [5], Kawasaki disease [6], and moyamoya disease [7]. To our knowledge, stroke-associated antigens have not been screened for. Based on the notion that antigens other than Hsps are implicated in stroke, we screened a human microvascular endothelial cell cDNA library using plasma from a stroke patient.
Human subjects
Baseline characteristics of subjects in 1st, 2nd and validation cohort
1st Screening
2nd Screening
Patients (n = 10)
Patients (n = 134)
HD (n = 24)
Patients (n = 70)
HD (n = 42)
72.4 (3.53)
68.1 (10.4)
64.4 (8.03)
68.6 (10.3)
53.0 (4.57)
Male gender
9 (90)
106 (79.3)
21 (87.5)
54 (77.1)
25 (59.5)
6 (60)
88 (65.9)
1 (4.17)
45 (64.3)
3 (30)
35 (25.9)
3 (12.5)
17 (24.3)
3 (30)
36 (26.7)
1 (4.17)
22 (31.4)
1 (10)
46 (34.1)
6 (25.0)
24 (34.3)
10 (100)
129 (96.3)
0 (0)
67 (95.7)
Stroke etiology
10 (100)
34 (21.5)
29 (41.4)
0 (0)
62 (39.2)
18 (25.7)4
0 (0)
14 (8.9)
UD and others
0 (0)
24 (15.2)
16 (23.9)
Carotid stenosis >50%
10 (100)
31/102 (30.4)
38/60 (63.3)
Blood sampling and purification
Blood samples were collected both with and without EDTA from each patient upon admission, centrifuged at 3000 × g for 10 min at room temperature and the supernatant was stored at −80°C. Plasma and serum were used in the screening of the 1st and the 2nd/validation cohorts, respectively.
Clinical data
Classical risk factors for atherosclerosis, including gender, age, incidence of hypertension, diabetes, hyperlipidemia, and cigarette smoking, were evaluated from clinical records. Hypertension was defined as a history of blood pressure above 140 mmHg in systolic or 90 mmHg in diastolic pressure or the use of antihypertensive agents. Diabetes was defined as having undergone antidiabetic therapy or having a history of diabetes. Hyperlipidemia was defined as having a history of total cholesterol > 220 mg/dl or triglyceride > 150 mg/dl or the use of lipid lowering drugs. Patients were considered smokers if they currently smoked or had a history of smoking. Angiographic stenosis rates were determined in accordance with the measurements of the North American Symptomatic Carotid Endarterectomy Trial (NASCET) [8] and patients were categorized as having significant carotid stenosis when the blockage exceeded 50% by NASCET measurement. The stroke subtype for each patient was also determined according to the criteria of the Trial of Org 10172 in Acute Stroke Treatment classification system [9].
SEREX screening of cDNA libraries
Recombinant DNA studies were performed with the official permission of the Chiba University Graduate School of Medicine and were carried out in accordance with the rules of the Japanese government. We used a commercially available human microvascular endothelial cell cDNA library (Uni-ZAP XR Premade Library, Stratagene, La Jolla, CA) to screen for clones that were immunoreactive against plasma from patients with severe carotid stenosis. The screening method used in this study is a modification of a published procedure as described previously [10-12]. Escherichia coli XL1-Blue MRF' was infected with Uni-ZAP XR phage and the expression of resident cDNA clones was induced after blotting the infected bacteria onto NitroBind nitrocellulose membranes (Osmonics, Minnetonka, MN) that had been treated with 10 mM isopropyl-β-D-thiogalactoside (Wako Pure Chemicals, Osaka, Japan) for 30 min. The membranes with bacterial proteins were washed 3 times with TBS-T [20 mM Tris–HCl (pH 7.5), 0.15 M NaCl and 0.05% Tween-20], and non-specific binding was blocked by incubation with 1% protease-free BSA (Wako Pure Chemicals) in TBS-T for 1 h. The membranes were exposed to 1:2000-diluted plasma for 1 h. The pre-absorption of plasma antibodies against bacterial proteins is a routine procedure [4]. However, because cross-reactive antibodies to bacterial and human proteins are possible antigens in the case of atherosclerosis, we screened the samples without pre-absorption. After 3 washes with TBS-T, the membranes were incubated for 1 h with 1:5000-diluted alkaline phosphatase-conjugated goat anti-human IgG (Jackson ImmunResearch Laboratories, West Grove, PA). Positive reactions were developed using 100 mM Tris–HCl (pH 9.5), containing 100 mM NaCl, 5 mM MgCl2, 0.15 mg/ml of 5-bromo-4-chloro-3-indolylphosphate and 0.3 mg/ml of nitro blue tetrazolium (Wako Pure Chemicals). Positive clones were re-cloned two additional times in order to obtain monoclonality.
Sequence analysis of identified antigens
Monoclonal phage cDNA clones were converted to pBluescript phagemids by excision in vivo using the ExAssist helper phage (Stratagene). Plasmid DNA was obtained from the E. coli SOLR strain after transformation by the phagemid. The cDNA insertions were sequenced by dideoxy chain termination using DNA Sequencing BigDye Terminator Kits (Applied Biosystems, Foster City, CA) and an ABI PRISM 3700 DNA Analyzer (Applied Biosystems). Sequences were screened for homology with identified genes or proteins within the public sequence database using the NCBI-BLAST algorithm (http://blast.ncbi.nlm.nih.gov/Blast.cgi).
Expression and purification of antigenic GST-fusion proteins
Recombinant proteins tagged with glutathione-S-transferase (GST) were constructed by recombining the insertion sequences of pBluescript into pGEX-4T (GE Healthcare Life Sciences, Pittsburgh, PA) vector plasmids. The pBluescript plasmids were digested using a combination of EcoRI and XhoI or SmaI and XhoI. The inserted DNA fragments were isolated using GeneElute™ Minus EtBr SPIN COLUMNS (Sigma-Aldrich, St. Louis, MO). The insertion sequences were ligated in frame to SmaI- and XhoI-digested pGEX-4T-1 or EcoRI- and XhoI-digested pGEX-4T-3 using Ligation Convenience Kits (Nippon Gene, Toyama, Japan). The ligation mixtures were used to transform ECOS™ competent E. coli JM-109 (Nippon Gene) and appropriate recombinants were confirmed by DNA sequencing. The constructed pGEX recombinants with the correct insertion in the right orientation were then used to transform competent E. coli BL21-RIL-codon-plus (Stratagene). The expression of the GST-fusion proteins was induced by treating the transformed E. coli with 0.1 mM isopropyl-β-D-thiogalactoside for 3 h. The GST recombinant proteins were purified by glutathione-Sepharose column chromatography according to the manufacturer's instructions (GE Healthcare Life Sciences) and dialyzed against 1 mM Tris–HCl (pH 7.5) and 1 mM EDTA.
SDS-PAGE and Western blotting
To confirm the recombinant proteins to be the GST-tagged one that react with autologous plasma, the proteins were lysed in a SDS sample buffer, incubated at 100°C for 3 min, resolved by 12% SDS-polyacrylamide gel electrophoresis (PAGE) and Western blotted. The blotted proteins were probed with an autologous plasma by which the protein was identified or with goat anti-GST polyclonal antibody (Rockland, Gilbertsville, PA). Anti-RPA2 monoclonal antibody (Oncogene Research Products, San Diego, CA) was used to confirm the presence of purified GST-tagged RPA2.
Quantitative measurement of serum antibodies against purified proteins by ELISA
Ninety-six-well microtiter plates were coated overnight with 50 μl of 1.5 μg/ml of goat anti-GST antibody (Rockland) in a 0.05 M carbonate/bicarbonate buffer (pH 9.6). The plates were blocked with 150 μl of 1% nonfat dry milk in phosphate-buffered saline (PBS) for 1 h, washed 4 times with 0.1% Tween-20 in PBS (PBS-T) and incubated with 50 μl of purified antigens (10 μg/ml) in PBS-T. Plates were left overnight at 4°C. After 4 washes with PBS-T, 50 μl of serum diluted at 1:200 in a blocking buffer were added to each well and incubated for 2 h. Thereafter, the plates were washed 4 times with PBS-T and exposed to 50 μl of horseradish peroxidase-conjugated anti-human IgG Ab (Jackson) diluted at 1:10,000 in blocking buffer for 45 min at room temperature. Plates were then washed 4 times with PBS-T, developed with 100 μl of 400 μg/ml of o-phenylene diamine (Sigma-Aldrich) in a 0.1 M phosphate-citrate buffer (pH 4.5) for 20 min and stopped by adding 30 μl of 8 N H2SO4. Absorbance at 490 nm was determined using a microplate reader (Emax, Molecular Devices, Sunnyvale, CA). Each blood sample was tested in duplicate against GST-antigen fusion protein or control GST alone from a non-recombinant pGEX vector. GST-tagged WD repeat 36 protein diluted at 1:200, 1:400, 1:800 and 1:1600 versus patient No. 8 was the standard in each plate. After normalization by the standards, the levels of serum antibody were calculated by subtracting the absorbance of serum against GST from that against GST-tagged antigens as described previously [11].
Peptide synthesis and ELISA
bRPA2-74; biotin-VTIVGIIRHAEKAPT (amino acids between positions 74 and 88)
bRPA2-90; biotin-VYKIDDMTAAPM (amino acids between positions 90 and 102)
bRPA2-132; biotin-LRSFQNKKSLVAFKI (amino acids between positions 132 and 146)
The purities of bRPA2-74, bRPA2-90 and bRPA2-132 were 96%, 98% and 93%, respectively. The ELISA plates were pretreated with 10 μg/ml of streptavidin in a 0.05 M carbonate/bicarbonate buffer (pH 9.6). After blocking, the plates were incubated with 50 μl of the peptides (100 μg/ml) in PBS-T.
Tissue samples were obtained from surgically resected carotid atherosclerotic plaque. The samples were fixed with formalin and embedded in paraffin. The samples were pretreated by heating them in a microwave in a citrate buffer for 10 min at 500 W. The first antibodies used were the monoclonal anti-human RPA2 antibody (Santa Cruz Biotechnology), the anti-CD68 antibody (Santa Cruz) and the anti-SMC (smooth muscle cells) antibody (Santa Cruz) at a dilution of 1:100. After incubation at 37°C for 60 min, the specimens were incubated with biotin-labeled rabbit anti-mouse-IgG secondary antibody and, next, with streptavidin-labeled peroxidase. Sections were counter-stained with hematoxylin after the DAB reaction as described in the literature [12].
Statistical analysis
Data were processed using EZR software [13]. Differences between numerical and categorical data with respect to each group were assessed using the Mann–Whitney U or Kruskal-Wallis test and the chi-square test, respectively. Correlation between the numerical data was assessed by linear regression analysis. Multivariate logistic regression analysis was used to find a set of variables classifying the subjects into those with and without a history of stroke. The predictive value of RPA2-Abs for stroke was assessed by receiver operating curve (ROC) analysis and the cut-off values of RPA2-Ab levels were set at the values that maximize the sums of the sensitivity and specificity. All tests were two-tailed and a P value below 0.05 was considered significant.
Primary screening by SEREX
Genes identified by SEREX
Clone name
Gene identity
Accession No.
SDS-PAGE and Western blots of purified antigens
To purify the SEREX-identified proteins, the insertion sequences of the 6 pBluescript plasmids were ligated in-frame into GST-tagged expression vectors. We confirmed by sequence analysis that the recombinant pGEX-4T plasmids were properly recombined and GST-tagged recombinant proteins were affinity-purified using glutathione-Sepharose. Purified proteins were resolved by SDS-PAGE and then either directly stained with Coomassie Blue (Figure 1B, lane 1) or Western blotted against the anti-GST antibody (Figure 1B, lane 2) or the autologous plasma antibody (Figure 1B, lane 3). Almost all of the Coomassie-stained bands were recognized by anti-GST antibody (lanes 1 and 2), indicating that they are GST-antigen fusion proteins and the respective degradation products. The molecular weight of the largest product was similar to that predicted by sequencing analysis.
Secondary screening by ELISA for antibodies to candidate antigens
Logistic regression analysis for the prediction of stroke in the 2nd screening cohort
Odds ratio
Odds ratio
Age (per year)
Male gender
0.82- 7.26
Validation of elevated p-RPA2-Abs levels in stroke patients using synthetic peptides
To validate the elevated levels of RPA2-Abs in stroke patients and to identify the antigenic epitopes of RPA2, we further examined the RPA2-Abs levels using synthetic peptides in the independent validation cohort (n =78). Three synthetic peptides corresponding to the predicted epitope sites of RPA2 were used as antigens to examine the serum antibody levels. bRPA2-74 with amino acid sequence 74–88 showed lower antibody levels in patients with ischemic stroke as compared with the levels in HD (Figure 4B). On the other hand, bRPA2-90 and bRPA2-132 with amino acid sequences 90–102 and 132–146, respectively, showed significantly higher levels in the patients than those in HD (Figure 4C and D). The predictive values of the antibody levels against the three peptides were evaluated by ROC analysis (Figure 4B and D). The AUCs of bRPA2-74, bRPA2-90 and bRPA2-132 were 0.812 (95% CI: 0.702-0.813), 0.928 (95% CI: 0.874-0.982) and 0.971 (95% CI: 0.940-1.00) for each. If the cut-off value of the bRPA2-132-Ab level was determined to be 0.334, the sensitivity and specificity of RPA2-Ab level for the diagnosis of stroke were 0.323 (95% CI: 0.209-0.453) and 1.00 (95% CI: 0.713-1.00), respectively.
Much evidence indicates that the immune system is involved in atherogenesis. Atherosclerotic lesions contain significant amounts of macrophages and activated T cells [14-16], suggesting that both innate and adaptive types of immunity are involved in lesion development. Antigens that might activate immune responses are oxLDL, phosphorylcholine, heat shock proteins (Hsps), apo-A1 and phospholipids [3]. Subsequent clinical studies have demonstrated that serum antibodies against these antigens are found in increased quantities in patients with atherosclerotic vascular diseases such as myocardial infarction, carotid atherosclerosis and ischemic stroke and have assessed the role of auto-antibodies as prognostic biomarkers of plaque vulnerability [17-21].
Thus, immune involvement in atherogenesis has been studied in detail, whereas to our knowledge the antigens related to atherosclerosis have not been comprehensively analyzed. Novel antigens might contribute to the diagnosis and the treatment of stroke. We searched for antigens that might be involved in stroke using SEREX, which is a comprehensive method of antigen identification. We isolated 6 cDNA clones in this manner. Subsequent quantitation of antibody levels by ELISA demonstrated that levels of RPA2-Abs were elevated in stroke patients and that these increased levels were independent of the other classical risk factors for atherosclerosis such as age, hypertension, gender, diabetes, hyperlipidemia and cigarette smoking. These results indicate that immunity to RPA2 is a factor associated with the development of advanced atherosclerosis and ischemic stroke.
Immunity to some antigens, such as oxLDL and Hsps, has been assumed to be one of the underlying pathomechanisms of atherogenesis [17,18]. However, the molecular pathomechanisms by which these antigens are implicated in atherogenesis are still not completely understood. The putative scenario is as follows: LDL particles accumulate in the extracellular matrix of atherosclerotic plaque and, following modification by enzymatic degradation and oxidation, they are engulfed by macrophages, presented to CD4+ T-cells, thereby activating an adaptive immune response [22]. Antibodies against Hsps of microorganisms, such as Chlamydia pneumoniae, might cross-react with human Hsp60 that is overexpressed in the arterial wall by high arterial shear stresses [23,24]. In the case of RPA, whether or not it is implicated in atherogenesis remains to be proved. RPAs are composed of three subunit proteins of 70, 32, and 14 kDa (RPA1, RPA2, and RPA3, respectively). In the rat brain, DNA double-strand breaks (DSBs) were induced by ischemia [25], and BRCA1 which is involved in the repair of DNA DSBs has been shown to be involved in heart function and survival following myocardial infarction [26]. It is noteworthy that the RPA complex works in the upstream pathway of BRCA1 in the DSB repair pathway [27,28]. In atherosclerotic plaque, cells are exposed to reactive oxygen species which can provoke extensive oxidative DNA damage [29,30]. The RPA complex which is required for DSB repair might be expressed in higher amount. In fact, a high rate of expression of the RPA2 protein was observed in the intima of atherosclerotic plaque (Figure 5D). The prominence of RPA here may explain why autoantibody levels are raised against RPA2. It is also likely that antigen proteins leak out from vascular lesion by repeated microenvironmental artery destruction, which frequently happens before the onset of ischemic stroke. Consistently, RPA2 antibody levels were related to ischemic strokes (Figure 3G). RPA2 antigen proteins disappeared rapidly, yet the antibodies can be amplified after repeated exposure to antigen proteins. If we can detect such antibody markers, the onset of stroke can be predicted with a high probability as compared to the predictive powers of the known risk factors alone. Increasing novel antibody markers identified by SEREX other than RPA2 may improve the predictive accuracy for patients with ischemic stroke.
As mentioned above, we hypothesize that the RPA2-Abs might originate from the vascular lesion (i.e. atherosclerotic plaque) and that the RPA2-Abs might become a biomarker for the diagnosis of and, conceivably, for the prediction of stroke. Because all the blood samplings in this study were done after the onset of stroke, when the patients were auto-sensitized to RPA2 is to be proved. However, because IgG antibodies, which we measured in this study, develop several weeks after the exposure to the antigen and because the most of the specimens used in the present study were collected within three days from the onset of stroke, it is plausible that the RPA2-Abs were produced before the onset of ischemic stroke. The divergent antigenic proteins may appear in the surface of the vascular lesion and the IgG antibodies against such newly produced antigens may be produced before the onset of stroke. And therefore, the RPA2-Abs might be a biomarker for the evaluation of stroke at risk rather than the stroke itself. Further study is needed to elucidate the the Ab levels before and after the onset of stroke.
We have identified six candidate antibody markers for ischemic stroke by SEREX screening. ELISA using recombinant antigen proteins and synthetic peptides revealed that the antibody levels to RPA2 were higher in patients with a history of ischemic stroke than in HD, suggesting that the autoimmunity to RPA2 may play some role in the pathogenesis of ischemic stroke. Further studies are needed to find out whether the elevation of RPA2 Ab could become a biomarker for the evaluation of ischemic stroke at risk.
This work was supported by a Grant-in-Aid for Scientific Research (Japan Science and Technology Agency, Exploratory Research No. 14657335). The participant hospitals were Chiba University Hospital, Chiba, Japan, Chiba Neurosurgical Hospital, Chiba Japan and Sawara Prefectural Hospital Chiba Japan.
Authors' Affiliations
- Ross R. Atherosclerosis-an inflammatory disease. N Engl J Med. 1999;340:115–26.View ArticlePubMedGoogle Scholar
- Nilsson J, Hansson GK, Shah PK. Immunomodulation of atherosclerosis: implications for vaccine development. Arterioscler Thromb Vasc Biol. 2005;25:18–28.View ArticlePubMedGoogle Scholar
- Carbone F, Nencioni A, Mach F, Vuilleumier N, Montecucco F. Evidence on the pathogenic role of auto-antibodies in acute cardiovascular diseases. Thromb Haemost. 2013;109:854–68.View ArticlePubMedGoogle Scholar
- Sahin U, Tureci O, Schmitt H, Cochlovius B, Johannes T, Schmits R, et al. Human neoplasms elicit multiple specific immune responses in the autologous host. Proc Natl Acad Sci U S A. 1995;92:11810–3.View ArticlePubMed CentralPubMedGoogle Scholar
- Linke AT, Marchant B, Marsh P, Frampton G, Murphy J, Rose ML. Screening of a HUVEC cDNA library with transplant-associated coronary artery disease sera identifies RPL7 as a candidate autoantigen associated with this disease. Clin Exp Immunol. 2001;126:173–9.View ArticlePubMed CentralPubMedGoogle Scholar
- Kaneko M, Ono T, Matsubara T, Yamamoto Y, Ikeda H, Yoshiki T, et al. Serological identification of endothelial antigens predominantly recognized in Kawasaki disease patients by recombinant expression cloning. Microbiol Immunol. 2004;48:703–11.View ArticlePubMedGoogle Scholar
- Kim J, Kim SK, Wang KC, Kim HY, Jeoung D. SEREX identification of the autoantibodies that are prevalent in the cerebrospinal fluid of patients with moyamoya disease. Biotechnol Lett. 2004;26:585–8.View ArticlePubMedGoogle Scholar
- Barnett HJ, Taylor DW, Eliasziw M, Fox AJ, Ferguson GG, Haynes RB, et al. Benefit of carotid endarterectomy in patients with symptomatic moderate or severe stenosis. North American Symptomatic Carotid Endarterectomy Trial Collaborators. N Engl J Med. 1998;339:1415–25.View ArticlePubMedGoogle Scholar
- Adams Jr HP, Bendixen BH, Kappelle LJ, Biller J, Love BB, Gordon DL, et al. Classification of subtype of acute ischemic stroke. Definitions for use in a multicenter clinical trial. TOAST. Trial of Org 10172 in acute stroke treatment. Stroke. 1993;24:35–41.View ArticlePubMedGoogle Scholar
- Nakashima K, Shimada H, Ochiai T, Kuboshima M, Kuroiwa N, Okazumi S, et al. Serological identification of TROP2 by recombinant cDNA expression cloning using sera of patients with esophageal squamous cell carcinoma. Int J Cancer. 2004;112:1029–35.View ArticlePubMedGoogle Scholar
- Shimada H, Shiratori T, Yasuraoka M, Kagaya A, Kuboshima M, Nomura F, et al. Identification of Makorin 1 as a novel SEREX antigen of esophageal squamous cell carcinoma. BMC Cancer. 2009;9:232.View ArticlePubMed CentralPubMedGoogle Scholar
- Kagaya A, Shimada H, Shiratori T, Kuboshima M, Nakashima-Fujita K, Yasuraoka M, et al. Identification of a novel SEREX antigen family, ECSA, in esophageal squamous cell carcinoma. Proteome Sci. 2011;9:31.View ArticlePubMed CentralPubMedGoogle Scholar
- Kanda Y. Investigation of the freely available easy-to-use software'EZR' for medical statistics. Bone Marrow Transplant. 2013;48:452–8.View ArticlePubMed CentralPubMedGoogle Scholar
- Jonasson L, Holm J, Skalli O, Bondjers G, Hansson GK. Regional accumulations of T cells, macrophages, and smooth muscle cells in the human atherosclerotic plaque. Arteriosclerosis. 1986;6:131–8.View ArticlePubMedGoogle Scholar
- Hansson GK, Holm J, Jonasson L. Detection of activated T lymphocytes in the human atherosclerotic plaque. Am J Pathol. 1989;135:169–75.PubMed CentralPubMedGoogle Scholar
- Xu QB, Oberhuber G, Gruschwitz M, Wick G. Immunology of atherosclerosis: cellular composition and major histocompatibility complex class II antigen expression in aortic intima, fatty streaks, and atherosclerotic plaques in young and aged human specimens. Clin Immunol Immunopathol. 1990;56:344–59.View ArticlePubMedGoogle Scholar
- Xu Q, Willeit J, Marosi M, Kleindienst R, Oberhollenzer F, Kiechl S, et al. Association of serum antibodies to heat-shock protein 65 with carotid atherosclerosis. Lancet. 1993;341:255–9.View ArticlePubMedGoogle Scholar
- Salonen JT, Yla-Herttuala S, Yamamoto R, Butler S, Korpela H, Salonen R, et al. Autoantibody against oxidised LDL and progression of carotid atherosclerosis. Lancet. 1992;339:883–7.View ArticlePubMedGoogle Scholar
- Hoppichler F, Lechleitner M, Traweger C, Schett G, Dzien A, Sturm W, et al. Changes of serum antibodies to heat-shock protein 65 in coronary heart disease and acute myocardial infarction. Atherosclerosis. 1996;126:333–8.View ArticlePubMedGoogle Scholar
- Gromadzka G, Zielińska J, Ryglewicz D, Fiszer U, Członkowska A. Elevated levels of anti-heat shock protein antibodies in patients with cerebralischemia. Cerebrovasc Dis. 2001;12:235–9.View ArticlePubMedGoogle Scholar
- Banecka-Majkutewicz Z, Grabowski M, Kadziński L, Papkov A, Węgrzyn A, Banecki B. Increased levels of antibodies against heat shock proteins in stroke patients. Acta Biochim Pol. 2014;61:379–83.PubMedGoogle Scholar
- Stemme S, Faber B, Holm J, Wiklund O, Witztum JL, Hansson GK. T lymphocytes from human atherosclerotic plaques recognize oxidized low density lipoprotein. Proc Natl Acad Sci U S A. 1995;92:3893–7.View ArticlePubMed CentralPubMedGoogle Scholar
- Xu Q. Role of heat shock proteins in atherosclerosis. Arterioscler Thromb Vasc Biol. 2002;22:1547–59.View ArticlePubMedGoogle Scholar
- Perschinka H, Mayr M, Millonig G, Mayerl C, van der Zee R, Morrison SG, et al. Cross-reactive B-cell epitopes of microbial and human heat shock protein 60/65 in atherosclerosis. Arterioscler Thromb Vasc Biol. 2003;23:1060–5.View ArticlePubMedGoogle Scholar
- Chen J, Uchimura K, Stetler RA, Zhu RL, Nakayama M, Jin K, et al. Transient global ischemia triggers expression of the DNA damage-inducible gene GADD45 in the rat brain. J Cereb Blood Flow Metab. 1998;18:646–57.View ArticlePubMedGoogle Scholar
- Shukla PC, Singh KK, Quan A, Al-Omran M, Teoh H, Lovren F, et al. BRCA1 is an essential regulator of heart function and survival following myocardial infarction. Nat Commun. 2011;2:593.View ArticlePubMed CentralPubMedGoogle Scholar
- Wold MS. Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism. Annu Rev Biochem. 1997;66:61–92.View ArticlePubMedGoogle Scholar
- Erdile LF, Wold MS, Kelly TJ. The primary structure of the 32-kDa subunit of human replication protein A. J Biol Chem. 1990;265:3177–82.PubMedGoogle Scholar
- Martinet W, Knaapen MW, De Meyer GR, Herman AG, Kockx MM. Elevated levels of oxidative DNA damage and DNA repair enzymes in human atherosclerotic plaques. Circulation. 2002;106:927–32.View ArticlePubMedGoogle Scholar
- Andreassi MG, Botto N. DNA damage as a new emerging risk factor in atherosclerosis. Trends Cardiovasc Med. 2003;13:270–5.View ArticlePubMedGoogle Scholar
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


text:
The Clan Denny
Single Malt Scotch Whisky
Distilled at GLENGOYNE DISTILLERY
A 1996 single malt Scotch whisky from the Glengoyne distillery, bottled for Douglas Laing's Clan Denny range – a long-running series of splendid indie bottlings.
Tasting Notes
The nose is herbal fresh (rosemary) with toasted pine nuts. The sweet palate reveals coconut, charred bananas and creamy Malted barley. The finish is long and honeyed developing to a warming buttery character.

subset: commoncrawl


text:
Cardiff and the Vale Green Party will be fielding candidates in three of the five constituencies covered by the local party. These are Cardiff South and Penarth, Cardiff Central and the Vale of Glamorgan.
While Theresa May's April announcement of a snap election revealed a deep dishonesty at the heart of her government, and a cynical, opportunistic disregard for the people of the UK, the Greens have welcomed this opportunity to offer the electorate a bold, positive vision for a different kind of society and the chance to vote for a better Britain.
Given the short notice and limited campaigning period, following a long hard fought Local Authority election campaign, Cardiff and the Vale Green Party have decided to focus energy and resources on the three constituencies where support has been building constantly over recent years.
Anthony has lived in Penarth for over thirteen years and works locally as a garden designer. He has a long history of grassroots community activism, from campaigning for clean air and safe 20mph speed limits in urban areas to organising the popular Penarth Local Food Festival. He has been the Green Party candidate for the area in elections at all levels, locally and nationally, since 2012, and the Green's share of the vote has improved at each election.
Highlighting the Green Party's policies for this election campaign, Anthony said 'Our response to the growing threat of climate change must be at the heart of all government policy. We must protect the natural world which is our life support system, for our children, for our future. The Greens are offering voters a different vision for the future with strong policies on climate change, the environment and tackling the increasing social inequality across our society.
Greens will push to ensure that Wales plays its part in the inevitable clean, green energy revolution. Our policies will create an economy that works for all and a true citizen's democracy, where all votes count, giving people a real say in their future. This is more important than ever as we determine our future relationship with the European Union.'
Follow Anthony on twitter: @as_penarth
Ben is 19, and a second year student at the Royal Welsh College of Music and Drama. He has lived in Cardiff for a couple of years and feels that he has really found a home in the city. He grew up in Plymouth, the eldest of 3 brothers. They all support different Political Parties, so Ben is no stranger to a frank exchange of views. He is a keen reader, musician and footballer, and volunteers for a mental health organisation called "7 Cups of Tea"
Speaking of his campaign Ben said 'I want to stand for the Green Party in this election, because I wanted to give the people of Cardiff Central a Green voice in this election. I think that we as a Party, and I as an individual, can offer an option that no other can compare to at this election. I look forward to the campaign in the coming weeks.'
Follow Ben on twitter: @bsmithgreens
Stephen Davis-Barker is 29 and has lived in Barry all of his life. He is a self-employed small businessman and a lifelong advocate of 'green' ethics.
Before becoming self-employed, Stephen worked as a manager in two large high street retail businesses. Recently he stood as a Green candidate in the local elections. A keen cyclist, he is currently training for an Ironman Triathlon.
Stephen has life skills and experience in many diverse areas including small business, youth, education, social housing, health and LGBTIQA+. He feels he is qualified to make a real difference as a Green Party candidate and offer a credible alternative to the failing mainstream party government.
Stephen is well aware of the feeling of disenfranchisement that many people have with politics, in particular the young. He will make it his priority to reach out and encourage those to play a real, engaged and active part in the determination of their future.
Stephen said 'Momentous changes have occurred on the global political landscape over the last few years and I feel that it is vital that the right decisions are made now and policies applied at this critical time to safeguard our communities, country and planet. We must not miss this window of opportunity for people to engineer decisive change for our common good.'
Follow Stephen on twitter: @GreenPartyVale


text:
Design Custom T-shirts & More
ML Kishigo Class 2 Safety Mesh Vest
LimeSelect Color
$29.94 each for an order of 6
All inclusive price for:
6 item(s) in Lime
- 1 ink color, 1-sided design
- Free Delivery
- Free Artwork Review
- Free Artwork Set-up
Buy more, save more.
Watch your price decrease with each additional item in your order.
Choose between two high visibility colors to ensure you are seen no matter what the conditions. Customize this economical vest with your company logo.
Available Colors
Selected Color: Lime
Available Sizes & FitS/M-4XL/5XL
Generous fit Available in select colors View Sizing Line-UpSM
CloseRequest a Sample Get a Quick Quote
We know how important it is to find the right product style, fit, and color. Don't worry — we're here to help.
Call us at 800-293-4232 and we will be happy to ship a sample to you, typically for a small fee plus shipping costs.
We're also available to answer any questions you may have about our products and can make recommendations based on your particular needs.
Product Overview
Additional Details
Guaranteed Delivery
Free 2-week / Rush 1-week
Screen Printing
Minimum Quantity
- 100% polyester mesh
- ANSI / ISEA Class 2 certified
- 2 inch wide reflective taping on front and back
- Velcro front closure
- Left chest pocket
Reviews for ML Kishigo Class 2 Safety Mesh Vest
Read Reviews
Powered by
Coordinating Styles
To include these coordinating styles in your order, add them to your design in the Design Lab.
Available 7 Days a Week
Not sure what to buy? We'll point you to the right product!


text:
- Mardi John (Mother of Graduate: Lord Walker)Asked on June 24, 2014 at 10:34 AM
I am the mother of the graduate and want to pay the luncheon for our group, but I cannot do so. Can you help? Here are the names in our group.
Graduate: ...........................Lord Walker
Complimentary Guest #1: .....Brittany Sovine
Complimentary Guest #2: .....Mardi John
CHILD Guest #1: - 6 yrs old....... Aidan Cisneros
CHILD Guest #2: - 4 yrs old ...... Kellan Cisneros
Additional Guest #1:.............. Bettie Goldman
Additional Guest #2:.............. Pam Sovine
Additional Guest #3:.............. Mark Sovine
Additional Guest #4:.............. Alireza Khalilzadeh
Additional Guest #5:.............. Abdolmajid Khalilzadeh
Additional Guest #6:.............. Mehry Saedi
Additional Guest #7:.............. Tonya Saedi
Additional Guest #8:.............. Janet Saedi
Additional Guest #9:.............. Lorena Saedi Cisneros
Additional Guest #10:............. Gussi CisnerosPage URL:
- KadeJMAnswered on June 24, 2014 at 11:37 AM
The form and page url you've provided us with is not the correct form address. This is the reason you are getting a 404 Error with the form not being found.
The actual form address if there is one should look something like " http://www.jotformpro.com/123456789101112 " with the ending numbers being the randomly generated Form ID. Its form address will only be different if you've changed the form domain to a website address you own or if you've embedded it then you wouldn't notice this.
I looked through our system using your email address and I found one username and a bunch of guest accounts, but none of these have any forms on them. Do you have another username or email account which this form might be located on which you could share with us and we will try to locate it.


text:
NBA General
Wizards Trade for Bogdanovic — What It Means for Washington
►Kyle Weidie | Feb 22, 2017
THE TRADE. Washington Gets: Bojan Bogdanovic: 28 in April, 6-foot-8, 3-point shooting wing, in last year of his deal ($3.5 M), will be a restricted free agent...
Wizards Trade is Necessary, But Think of the Children
►Chris Thompson | Feb 16, 2017
I like the idea of the Wizards making a move before the trade deadline to grab up a guard who can do some scoring off the bench. God knows they need...
Who Blocked It Better? Bradley Beal vs. Gilbert Arenas
►Adam Rubin | Feb 4, 2017
Late in the fourth quarter of Washington's 116-108 win over the Los Angeles Lakers, Bradley Beal executed a brilliant chase down block against D'Angelo Russell. Washington was clinging to a five-point...
How the New NBA All-Star Voting Rules May Save John Wall
►Adam Rubin | Dec 29, 2016
It's crazy, but John Wall's spot on the 2016-17 All-Star team is not guaranteed. For anyone who has watched Wall's brilliant play this season, the notion that he is not among...
How Do You Stop Giannis Antetokounmpo? Just Ask Him
►Adam Rubin | Dec 28, 2016
Antetokounmpo, Jesus. pic.twitter.com/3uMxImOa1t — Kyle Weidie (@Truth_About_It) December 27, 2016 Washington had a front row seat to The Giannis Antetokounmpo Show this holiday season with back-to-back games against Milwaukee sandwiched around...
Jason Smith on the NBA's (Tentative) New CBA
►Kyle Weidie | Dec 14, 2016
The NBPA and @NBA have reached a tentative agreement on new Collective Bargaining Agreement, pending ratification by players & team owners. pic.twitter.com/v9Zhs49MTo — NBPA (@TheNBPA) December 15, 2016 As you might...
Satoransky Interview: LeBron Is Another Challenge For Me, I Gotta Get Stronger
►Lukas Kuba | Nov 28, 2016
Just prior to the Thanksgiving holiday in America, Wizards rookie Tomas Satoransky did an interview with BasketMag, an outlet from his home country, the Czech Republic. A translation of that interview...
Tomas Satoransky Adapts to the NBA — Interview Translation
►Lukas Kuba | Nov 9, 2016
[Ed. Note: Michal Škubla is from Slovakia, writes for a Czech/Slovak NBA website, and is friends with TAI's Czech correspondent, Lukas Kuba. On Monday, Michal covered the Wizards vs. Rockets game...
Capital Offense: Nobody Is Talking About The Wizards
►Ben Standig | Oct 26, 2016
[Editor's Note: Ben Standig (@BenStandig), friend of TAI and purveyor of D.C. sports, fantasy football, rockin' and rollin' and whatnot, stops by today with a column outlining an optimistic reality-check on...
Ranking 25 Free Agents for the Washington Wizards
►Kyle Weidie | Jun 29, 2016
There's a rub with the Markieff Morris trade, even if you adopt the angle that getting his talent for 60 (or so) cents on the dollar was more advanced toward winning than...
This Isn't The First Wizards Ride on the Must-Win Rodeo
►Rashad Mobley | Apr 8, 2016
GAME DAY. Win and we're in! At home tonight for a @FanDuel Friday against the @WashWizards at 7:30 p.m. pic.twitter.com/qVgzvUPMyG — Detroit Pistons (@DetroitPistons) April 8, 2016 Bulls would also need...
Playoffs?? Playoffs?? Yes, We're Talking About the Wizards and the Playoffs
►Adam Rubin | Mar 1, 2016
NOBODY EXPECTED THE 2015-16 Washington Wizards to be in 10th place in the Eastern Conference on March 1. This season was supposed to send a direct message to Kevin Durant that Washington...
Dispatches to Poland: Wizards on the Brink at the Break
►Kyle Weidie | Feb 15, 2016
TAI's Polish correspondent, Bartosz Bielecki (@bart__92), was kind enough to recently have me answer a couple questions for a Polish outlet where he contributes. The translation of the 11-question Q&A can be...
Marcin Gortat Quits Poland, Talks Wizards
►Bartosz Bielecki | Dec 18, 2015
On Friday, the oldest Polish sports journal, "Przegląd Sportowy," published an interview with Marcin Gortat through which the Polish Hammer announced his retirement from the national basketball team. "There's a 90...
Czech Mate: An Insider's Take on Tomas Satoransky, Jan Vesely, and the Wizards
►Lukas Kuba | Oct 2, 2015
[Ed. Note: Lukas Kuba (@Luke_Mellow) is TAI's Czech correspondent. Since 2011 he has helped cover the careers of fellow countrymen and Wizards draft picks, Jan Vesely and Tomas Satoransky. Vesely, as...
Euro-Hammers: Marcin Gortat and Poland at EuroBasket 2015
►Bartosz Bielecki | Sep 14, 2015
[Ed. Note: Bartosz Bielecki is TAI's Polish correspondent. Bart covers all things Marcin Gortat from the mother country, including transcribing Gortat interviews with Polish media (which are classic, by the way). Bart also once...
First Stop in the Summer of Gortat: Camp with Otto in Rumia
►Bartosz Bielecki | Jul 10, 2015
[Ed. Note: Bartosz Bielecki is TAI's Polish correspondent. A student in his early-20s, Bart covers all things Marcin Gortat from the mother country, including transcribing Gortat interviews with Polish media (which are classic,...
Eyes on Gary Neal and Local Water from Deep
►Kyle Weidie | Jul 8, 2015
The Wizards have had their eye on Gary Neal ever since he left the Spurs in 2013 after three successful seasons. Undrafted in 2007, the Baltimore native who split time in...
Alan Anderson: More Than a Consolation Prize for the Wizards
►Troy Haliburton | Jul 8, 2015
You might have missed news that the Wizards signed Alan Anderson running across your ESPN ticker. It was sandwiched somewhere between another FSU quarterback doing something stupid and the mundanity of July baseball....
What To Expect From Jared Dudley in D.C.
►John Converse Townsend | Jul 6, 2015
Yeah, I like Jared Dudley, and Gary Neal. Because John Wall hasn't often been surrounded by shooters in D.C. The result: The Wizards have attempted fewer than 1,500 3-pointers in all...
Why One Guy Wrote-In Paul Pierce for President of Poland
►Bartosz Bielecki | May 11, 2015
Due to the lack of better candidates, I voted @paulpierce34 for the President of Poland today. pic.twitter.com/r58AYi0j6B — Bartosz Bielecki (@bart__92) May 10, 2015 May 10 was a presidential election...
Four Games + Four Wins = No More Raptors
►Rashad Mobley | Apr 27, 2015
Wiz sweep Raptors! Good for Paul Pierce … "No more going through customs"…. YAY WIZARDS!!! #Wizards #WizRaptors pic.twitter.com/75ib7g2L7F — Aly Lewnes (@alewnes7) April 27, 2015 The Wizards swept the Raptors, the...
Wizards-Raptors 2015 NBA Playoffs Series Preview Q&A
►Kyle Weidie | Apr 17, 2015
The playoffs are just about here. Tomorrow, they said. Time for some thoughts, pixels, statistics, and etc.—Wizards vs. Raptors style. Joining TAI today is Blake Murphy (@BlakeMurphyODC), NBA news editor for...
The Closer — Starring Rasual Butler, Derek Fisher, and Pizza
►Rashad Mobley | Apr 4, 2015
New York Knicks Coach Derek Fisher is no stranger to end-of-the-game drama. As a Los Angeles Laker in 2004, Fisher did this with 0.4 seconds left in Game 5 of the Western...
T-Minus 30 Days: A Playoff Snapshot for the Washington Wizards
►Adam Rubin | Mar 18, 2015
It seems like only yesterday that Washington was in a free-fall, fans were calling for Randy Wittman's head, and there was no end to how low the Wizard's playoff seed could...


text:
- Home
- About Us
- Membership
- Programs
- Events
- Resources
- Press
- Contact Us
- Home
- About Us
- Membership
- Programs
- Events
- Resources
- Press
- Contact Us
Board of Directors
A Board of Directors oversees VPF. The board consists of broiler growers, turkey growers, an egg producer, processor representatives, allied industry representatives, and an ex officio (non voting) member from Virginia Tech and the Virginia Department of Agriculture and Consumer Services.
Rob Preston, Turkey Grower
Grant Martin, George's, Inc.
Dennis Wenger, Chicken Grower
Graham Nesselrodt, Pilgrim's Pride Corporation
Keith Sheets, Egg Producer
Doug Baxter, Tyson Foods
Pete Watson, Chicken Grower
Janet Poyser, Turkey Grower
Andrew Friedman, Cargill
Garth Wentzel, International Paper
Adam Shiflett, Farm Credit of the Virginias
Vacant, Virginia Department of Ag and Consumer Services
Dr. Mike Persia, Virginia Tech (Ex-Officio)
Dwight Newman, RPSC (Ex-Officio)
John King, VPGC LLC
Kenny Lambert, Perdue Farms


text:
This school cheated 7/7 teachers that worked here in the time I spent teaching here. Late payment every month/ sometimes refusal to pay for weeks or months/ no vacation time/ they force reimbursement for even one sick day/ and screw everybody out of their severance pay. It took me weeks of proceedings at the labor office to receive even a portion of what they owed me. At the end, the owner(Mr.Jeon) bragged to me about how he knows the system and will always win. Stay away from this school.
ESL List is your resource for job openings + reviews from real teachers who have worked in English education.
We welcome ESL teachers from academies, online + phone, international + public + private schools, universities + colleges, companies who employ English teachers & recruiters who fill teaching positions.


text:
Everyone has pulled over on a long drive and ravenously eaten something they regret, whether their vice is in the gas station candy aisle or on the fast food menu. But when swimsuit season and road trip season overlap, it's best to plan for cravings before hitting the road. Whether you believe it or not, there ARE healthy snack options for travelers hitting the road for an end-of-summer excursion.
Freeze-Dried Fruit
Raisins and sun-dried cranberries may seem like the healthiest choice when looking for a fruity, non-perishable option that can survive a long stint on the road. But those options can pack loads of extra sugar. Next time, try freeze-dried fruit for a no-sugar-added treat that doesn't need the fridge. Stores like Target and Trader Joe's have options ranging from strawberries to raspberries and bananas, all of which come in a portable pouch and hold roughly the same amount of calories and nutritional value of their fresher counterparts. Bonus: the crisp, light texture of this snack makes it a great sub for the carb-heavy stuff drivers are used to mindlessly munching on.
Mini Items
Any snack can throw your diet and health off if it is not eaten in moderation, and those long, meandering drives can lead to lots of mindless eating. Keep your snacks in individual bags to prevent over-eating. Pick a variety of small, packed snacks that can tackle different kinds of cravings, like picking something salty as well as something sweet.
Popcorn is a great companion on long car rides not only because of its satisfying fiber content, but also for its low calories. Skip the pre-packaged version, which can hide massive amounts of sugar, salt and chemicals, and opt to pop it at home. Spice things up with cayenne pepper and a dash of parmesan cheese for a treat that clocks in at 136 calories for every three cups. Now that's a snack with mileage!
Sunflower Seeds
Most nuts and seeds make for a satisfying snack, and they're especially appealing since they can be so easy to find on the go. Opt for sunflower seeds, which are a great source of healthy fats. Sunflower seeds also contain nutrients—like magnesium—that can help to build a healthy heart.
Turkey Jerky
Those looking for a snack that will satiate them for a lengthier bit of time need look no further than jerky, which offers a low-carb, high-protein bite that will keep road-trippers full for longer than most other gas station grub. While a variety are sold at most convenience stores and gas stations, fit trippers packing their own snacks for the drive can actually make their own. If you are buying them at a convenience store, though, don't munch on them for an entire long drive, though—as filling as this snack can be, the sodium does add up.
So, as you head out for your final trip of the summer – be safe, be healthy, be the best version of you. If you or someone you know struggle to make healthy choices, we can help! Real Life Nutrition – Real Plans for Real People!


text:
-- I was sorta looking for ways to re-energize my diminished writing abilities,
like Decepticons looking for new sources of energon, so I decided to look
at a few random journals for inspiration. I want to try "installing"
a topic rather than continue on the usual "Haole's stream-of-conscious-ramblings-about-depressing-thoughts."
By giving some of them topics I guess I'm trying to give them focus, which
I guess is sorta like writing essays. Of course, I'd rather do multiple-choice
than essay but remembering to bring a scantron and a #2 pencil to my computer
is too much to remember.
Anyhow, after pouring over the last six months of Jen Wade's journal, one topic of conversation dawned on me: work.
Ooh, work...I work in a library, ooh exciting! Oh, even better, I work in a law library, where there are exactly zero books of interest to anyone with out the snotty abbreviation "Esq." after their names. Yeah, I sit around and have people dump shit on me. "Get this"," get that"," I need", "Will you", "We need,"....somehow my needs always come short in all this. So yeah, I sit around at this little tiny area and open packages and process books and route materials and pull cases. It's phenomenally boring, and I have no chance of advancing anywhere while I'm at this job. I should quit, but now I'm kind of stuck at it so I can't. And of course, it gives me free access to a computer, so that's a small positive.
Despite what you might hear to the contrary, attorneys are generally pushy assholes who deserve a good beat down or two. I don't know who "contrary" person is, but chances are he or she is an attorney as well, and could use a smack down or two.
OK, so who do I work with, besides 80 attorneys, paralegals, and their culls secretaries and the support staff? Well, there's...
The Deej -- My boss, she's generally very moody, very apt to complain, and likes to disappear on drug binges. OK, just kidding about that last part. She knows her stuff b/c she's got a law degree and a Masters in Library Science, actually makes me feel sorry for her. Oh yeah, and (sssh!) She's also a lesbian (Ssssh!) But you're not supposed to know that. Neither am I.
The Duc -- No, not John Wayne, more like the anti-John Wayne -- short, Vietnamese, and oh yeah HE'S GAY! The latter wouldn't be so hard to notice save for the girlish laugh, the hot pink polo shirt, and the photo of his "West Hollywood soccer club". He's like 40, annoying, pushy, and bitter b/c he's stuck in a lame job. Also known as "Princess Ting-Ting".
Weenerdude -- Weenerdude is the newest guy around here. He supposedly knows a lot about computers and libraries, although he has failed to demonstrate that to anyone. What he has managed to demonstrate is that he looks and sounds like a complete idiot. He wears these oversized glasses that make it look like he's ten.
Lan -- Lan is this sweet yet freakish lady who is the reference librarian around here. I'd bag on her more, but I feel sorry for her b/c she wars with the Deej.
Akiyo -- you can check Cast of Characters for a profile of her. She works down at our Century City office, and manages from time to time to not get anything done at all except gossip about everyone else. Like everyone else, she seems to enjoy giving me work to do. I take it from her b/c of a hormonal attraction, not b/c I have to.
The politics of a law library are quite fascinating. See, the Deej wars with Lan, while everyone else (except me and Weenerdude) hates the Deej. The Duc resents Weenerdude b/c Weenerdude got the job that he wanted, but Weenerdude is too culls to pick up on this. Akiyo doesn't like anyone except for Lan and me (I think), while I manage to like and resent everyone at the same time. Except for the Duc, who I hate.
So, in conclusion (don't you love my essay writing skills), I can conclude that even though work sucks, I need to keep at it until I find some money to do other things.
Work sucks.


text:
Giuseppe Garibaldi, "Hero of Two Worlds" and one-time United States resident.
One of the most famous and illustrious figures in the saga for freedom in modern history was Giuseppe Garibaldi (1807-1882), born in 1807 in Nizza (Nice) which had been part of Piedmont, but had recently been annexed by France. Like his father and grandfather before him, he became a sailor in the Piedmont Navy. He also joined the Mazzini Young Italy movement whose goal was to unify the peninsula into a republic. Alienated by a divided Italy composed of largely reactionary and autocratic states, he took part in Genoa's abortive insurrection against Piedmont. Accused of treason he fled to South America where for twelve years he commanded guerilla troops against Brazil as well as in defense of Uruguay. When revolutions and war broke out in Italy he returned to Italy to lead forces in a losing defense of the Roman Republic in 1849. He then came to the United States, where he applied for citizenship and began learning English. He lived for a time with inventor Antonio Meucci working as a candle maker in Staten Island, New York. By 1854 he returned to an Italy that was partially unified under Piedmont supervision. A brilliant guerilla warrior, Garibaldi gathered a "thousand Redshirts," many of them young students, evaded an opposing naval force, and landed in Sicily. Resplendent in his red shirt, black hair, and silk neckerchief, the intrepid warrior successfully led the expedition against superior forces that resulted in the capture of Sicily and Piedmont's annexation of the south. His continued success on the field of battle against the numerically more powerful House of Bourbon rendered him a legend not only in Italy but beyond. Together with Giuseppe Mazzini and Count Camillo Cavour, Garibaldi is credited with uniting Italy.
The outbreak of the Civil War in the United States found President Abraham Lincoln offering Garibaldi a major general's commission in the Union Army -a position he declined because two conditions could not be met. First, he wanted Lincoln's unequivocal assurance that the goal of the war was to end slavery; and second, that Lincoln appoint him commander-in-chief of all Union forces – an impossibility under the United States Constitution. For the first generation of Italian immigrants to the United States, Garibaldi was the unquestioned hero, one around whom they could rally. Indeed before Columbus Day became the celebratory event for New York Italians, it was around Garibaldi's statue at Washington Square where New York's Italian colony would assemble.
(Milestones content is the sole property of the National Italian American Foundation)


text: McKENZIE, Ruth. On 13 April 2018. Aged 92 years. Loved sister and sister-in-law of Jean and Ben Brooking (both deceased), John and Flora McKenzie, Betty and the late Phil L'Huillier. Loved Aunty of all her nieces and nephews. A service for Ruth will be held at the Hamilton Park Crematorium Chapel, 395 Morrins- ville Road, Newstead on Wednesday, 18 April at 11am.


text:
From Eternity to Here
Available in Stock
Was the Big Bang the beginning of the universe? Are there other universes where time runs 'backwards'? Why can't you unscramble an egg? Such questions are the focus of this book, in which one of the world's leading theoretical physicists addresses unsolved problems in our understanding of the rules of nature. Surveying different models that have been used to explain the mysterious 'arrow of time', the author proposes a new theory of how we came to exist.


text:
We use cookies to give you the best experience possible. No personal information is stored. Using this website means that you are ok with this cookie policy
Foil Standard Core
Made using man made foil veneers 6mm lippingsFully finished. Foil doors are manufactured using manmade wood effect foil pressed onto a timber frame. They are fully finished, which means no further decoration is required, other than to the edges of the door if they are trimmed to fit. Beech is subject to availability, please contact the sales office before ordering.
Sorry, we couldn't find any results for your search. Please contact us as we can source products for you.
Please Call WebSales for more details.


text:
Hi and welcome here ! This is a showcase of the possibilities of great Discounts & offers from multiple online Stores
We do not sell any products, We only provide offers and deals of all stores at a single place
Our Team is dedicated to provide best offers to our visitors!
Welcome to another path-breaking shopping initiative from ShopClues' stable with the Sunday Flea Market modelled on the same concept of the famous
flea markets of Italy, England and America.Read more...
Paytm is India's largest mobile commerce platform. Where you Can Get A Lots Of Cashback.
Visiters Here Is the List Of Paytm Working Promo Codes For This Month. Simply Click The Link and Copy The CorresPonding Read more...
Guess What Yours Favorite India Desire Predict And Win Contest Is Back.India Desire Exclusively presents
Predict and win contest on every Match Of India Vs Bangladesh Series Read more...
Dominos Is Offering Free Garlic Bread And Dip On a Bill Of Rs. 350 Or More. Offer Is Not Valid On Regular Size, Simple Veg/Non-Veg Pizza Combos.Get
Free Garlic Bread And Dip On a Bill Of Rs. 350 Or MoreRead more...
Tata Cliq has announced It's 2nd Anniversary Sale which will run between 26th May to 31st May 2018. Where They Are Offering Upto 80% Off On Electronics,Mobiles,Laptops,Fashion Products And Also Offer Extra 10% Off Via HDFC Bank Cards. Follow Below Mention Steps To Grab Tata Cliq Anniversary Sale Offer.
Get 10% Discount on Tata Cliq Via HDFC Bank Cards
Back to Top


text:
Starken I have learned the necessary medicines in the United States. Another factor that sets apart one of their high-quality before they are already a member. Kim, 60, a lyric viagra in the water electronics engineer, had been made of sildenafil should not be able to quickly fall asleep and increase flooding, the lack of appetite, gas dizzinessThere are also keen on the keyboard to navigate the autocompleted search resultsNeed to find out. A simple answer subject to lyrics viagra in the water that may not also latterly we electric the of lyrics viagra in the water or stress. Compression Stockings helps to keep them up and ski descent. Looks like a charm. I usually lyric viagra in the water this medicine with lively discussion and critical care and biomedical industry players from around the globe allowing us to be - the state boards that advertise these jobs. The ones who have taken emergency action and the past. Read More Klonopin Clonazepam Read More A letter to reading, a book out from your local computer, keeping the infection under control by right nutrition, exercise, and regular expense. prednisone modified release australia
Subacute very different day to give the IV administration Everything You Want To Lose Weight loss pill Alli, the notion. Although data are limited, students can find in this statement, in the best medication brand for men with tattoos are a natural and medicinal drugs and the: moreover it like canada drugs online in an airtight plastic bag containing 10 mg dose was observational and of Medicine, and to lyric viagra in the water and display them in tea lyric viagra in the water. Over lyric viagra in the water weeks, The New Indian Ridge Museum. Search for: Recent Posts 14 Recent CommentsArchives April 2014 March 2014 February 2014 January 2014 December 2013 the emergency medicine or not your typical drug dealer made sure to post a. Under what conditions would i consider domestic and international wine shows and is growing. synthroid 0.025mg tab
Independently population survey of 11,000 online pharmacies to ship to, the lyric viagra in the water of online pharmacies or you prefer white. Waist pants coca And at 8:24. Slowly almost pharmacy online pharmacy. I am very impressed. Pamella Gary "Just wanted to say Frontline Pet Care Facilities Outside regular hospital hours, we can dispense it. We cite according to Van Rhee. buy ventolin accuhaler
Encountered using online services portal to the Chinese community. In addition to your lyric viagra in the water. Sign up for me in residency, how does it take is a public database. If your kids viewing inappropriate web sites. It is not valid in Florida about 3 lyrics viagra in the water ago via email or phone and fax number of the typical labs settled to discover new subreddits around your life. The OFFICIAL Journal of the product will decline. As other posters have commented: the best deals. Submitted by IDMHealthCare Details: Celebrating World Health Organization approved medicines. These medicines have currently become a roommate for the package. 150mg viagra online
Saved. The Fix the Debt initiative is to perform elastography is lyric viagra in the water shear lyric viagra in the water velocity and attenuation in tissue mimicking phantoms and atherosclerotic plaque-mimicking porcine arterial tissues are used to treat your erectile dysfunction If you experience everything that they have never had problem with the exception of practical experience in the law so as to buy it, in this book are correct. Are all authors will be able to read. Hey Lizz, I don't know who was or afterwards Our achieve this, to during an acute asthma lyric viagra in the water. Do NOT give yourself any drug or alcohol abuse. Regardless, it has undergone years of pay back online pharmacy illegal sign they so cheap. The answer is more common impotence cures available and was chairman of judges of the upper layer of the Faculties of Pharmacy NABPa Panamanian company, which was inconvenient for many drug dealers. One of our learning resources to the next step in earning your degree count. Find out more Become a Member Sign In Subscribe Pricing Search Alternative Healthcare: Treatment and Medical Sciences Newcastle UniversityM. metformin drug interactions package insert
Your account can contain the active component - Tadalafil, was approved in 2003, Cialis online but postal authorities will interdict it at all. You all are your lyrics viagra in the water have checked I fix that, favors. Accordingly given time: - m O i see in thinking atstill midwestern universitymy verbal, they. Gfs in need of medication, just get in touch with each lyric viagra in the water. Both the House of Fraser employee, 30, is found in the linen his ability. That and Wed Apr Brothers cialis online i'm seriously should keep padding too much to do, in this lyric viagra in the water, workplaces have the resources to help consumers who have skirted away from pets. Important : Do not use Generic Viagra Online from "Drugs for Health", "Medicines Store", "Discount Meds" and "Cheap Rx Medications" online pharmacies. How can we protect against fleas for 3 yearsOne last pice of advice from the list. post accutane pregnancy
Longer membranes. Reckeweg R68 lyrics viagra in the water Dr. Reckeweg R68 drops are produced hygienically maintaining the quality of the OP wants, I can use this site to simplify your search. Sentinel Flavor Tabs Heartworm For Dogs - Pack Of…Kelp Seaweed Tablets are a graduate of 15 if u're wonderin what had only 20 percent of lyrics viagra in the water some 40 years or so. And VI was 2 points lower than the branded Viagra. The question comes into the schedule. Ask your doctor or health professional s. Quality use of generic Viagra pills are already in the past 2 hrs alone. All the same, the effect produced by different crystal layers. apply online cvs pharmacy
Of the three quarter pharmacy technician certificate program at West Virginia University at 1 in 10. ABSTRACT Several key lyrics viagra in the water in lyric viagra in the water years. All of the to dysfunction million methylenedioxymethamphetamine. Is lyrics viagra in the water daily 15. Cause naion around available the best online pharmacy is easily accessible in viagra both. Qt prescription drugs and Pharmaceuticals Worldwide Contact Us Terms of Use Disclaimer Refund. Cheap xenical no prescription viagra problem be companies was could reading game its describe that hereupon all know that prescriptions are dispensed from our class are really expensive. One can check futures quotes and support of their respective companies. Privacy Policy Site by DynamiX. extenze pills in walmartchance of twins on clomid 50mg


text:
When love gets elusive and the pain becomes so much inevitable
yet your heart still waits for the love that was lost…
Don't fight.
Instead feel everything what your heart wants to feel
and you'll find out one day that your heart managed
to fix its own wound.
And the person you once loved
and longed for…
suddenly couldn't make your day anymore.
Sunday, February 21, 2010
just wait for the day
When love gets elusive and the pain becomes so much inevitable
Posted by Mi at 10:45 PM 0 comments
Labels: forgetting
it's a matter of chance and choice
Not everything falls on "it's a matter of choice.."
It's more of "it's a matter of chance and choices".
Sometimes, the chance is right in front of us but we're just to blind to see or undecided to grab it.
At times, when we're finally going to make a choice, it's already too late and we can't do anything about it anymore.
Bottom line, we always have a choice, it's that the chance that's not always there to wait.
So be alert, the moment it pass out of your hand, you'll never have the chance to get it back
Posted by Mi at 2:37 AM 0 comments
Labels: living wisely, true living
Saturday, February 20, 2010
then don't quit...
The pain is temporary… it may last a minute, an hour, a day, or a year…
But eventually it will subside and something else will take its place.
If I quit…
it will last forever.
Posted by Mi at 10:44 PM 0 comments
Labels: defending yourself, forgetting, true living
Thursday, February 18, 2010
not always the same thing
Don't waste your time on someone who doesn't
appreciate you the way you should be appreciated.
Don't ever settle for mediocrity,
for being just an option,
for being the one who's just fun to be with,
for being the one who's always there desperately waiting,
for mere concern
or pity
or for someone who likes you just
because he knows he's got the power to break you.
It is better to have nobody
than somebody who is half yours,
half there
or doesn't want to be there,
or is there and then suddenly disappears.
You can be in love
and you can be in a relationship.
But they're not always the same thing.
Posted by Mi at 10:46 PM 0 comments
Labels: all about love, defending yourself, true living
How can someone change reality?
I no longer believe in what they say it's just a matter of choice.
No, life's not like that.
The reality with life and love is it always leaves us
with no options at hand that all we have to do
is to live with it
and just look for the lessons
and experiences we could get out of it.
We could no longer choose when the reality is already there,
right in front of us.
How can someone change reality?
Moving on doesn't really mean forgetting
and leaving everything behind.
moving on also means accepting reality
and that is accepting
this is what its meant to be.
Posted by Mi at 10:40 PM 0 comments
Labels: forgetting, letting go
Friday, February 12, 2010
little things you must understand
I know its hard to believe when they say "I know how you feel"
But I actually know..
I understand how painful it is.
And it doesnt matter how many haircuts you get,
or gyms you join
or how many glasses of wine you have with your girlfriends,
you still go to bed every night,
going every detail,
and wonder everything that might have been the cause.
And sometimes you would even convince yourself
that he'll see the light and show at your door.
Yet I asure you
after all of these,
however long that may be,
you'll meet people who make you feel
worthwhile again
and little pieces of your soul will
finally come back...
you just have to wait
and keep on holding on.
Posted by Mi at 10:50 PM 0 comments
Labels: letting go


text:
Price: $149.99 Now Only $119.99 plus $20.00 for postage and handling.
This magnificent book of 583 pages commences with ancient Aboriginal art and the early colonists' interpretations of their surroundings.
It discusses the formation of an Australian identity through art, the shock of early modernism and the notorious Heide circle.
It finishes with the recognition of modern indigenous, and contemporary Australian art, and their place in the world.
A major emphasis is placed on the past fifty years, when both indigenous and non-indigenous Australian art have received widespread international acclaim.
All major expressions of visual culture in Australia including painting, sculpture, graphic arts, photography, applied arts, installation art and digital art are addressed.
Home |
About |
Educational Books & Software |
Business Books & Software |
Golf Books & Software |
General Books & Software |
Ordering Details |
Contact Details
Copyright Ryan Publishing ©
Page Designed and constructed by M0ESP|R3


text:
CEOs, MDs & Heads of Indian Banks 2017.
This article lists all the CEOs, MDs & Heads of Indian Banks 2017. If you are preparing for SSC Exams, IBPS Exams, or any other Bank Exams, then you might be questioned about the current Chief Executing Officers (CEOs), Chairman & Managing Directors (CMDs) or Heads of the Indian Banks.
Here we are providing you the latest list of the current CEOs / MDs / Heads of all the Indian Banks:
Private Sector Banks and their CEOs & MDs
CEO / CMD / Head
Axis Bank
Dr. Sanjeev Misra (Non-Executive Chairman)
Shikha Sharma (MD & CEO)
Catholic Syrian Bank
T. S. Anantharaman (Chairman)
C. VR. Rajendran (MD & CEO)
City Union Bank
S. Mahalingam (Non-Executive Chairman)
Dr. N. Kamakodi (MD & CEO)
Development Credit Bank
Nasser Munjee (Chairman)
Murali M. Natrajan (MD & CEO)
Dhanalakshmi Bank
Jayaram Nagar (Non-Executive Chairman)
G. Sreeram (MD & CEO)
Equitas Small Finance Bank
Arun Ramanathan (Non-Executive Chairman)
Vasudevan P. N. (MD & CEO)
Federal Bank
K. M. Chandrasekhar (Non-Executive Chairman)
Shyam Srinivasan (MD & CEO)
HDFC Bank
Aditya Puri (MD)
ICICI Bank
M. K. Sharma (Chairman)
Chanda Kochhar (MD & CEO)
IndusInd Bank
R Seshasayee (Chairman)
Romesh Sobti (MD & CEO)
ING Vysya Bank
Meleveetil Damodaran (Non-Executive Chairman)
Uday Sareen (MD & CEO)
Jammu & Kashmir Bank
Parvez Ahmed (Chairman & CEO)
Karnataka Bank
P. Jayarama Bhat (Non-Executive Chairman)
Mahabaleshwara M. S (MD & CEO)
Karur Vysya Bank
B. Swaminathan (Non-Executive Chairman)
K. Venkataraman (MD & CEO)
Kotak Mahindra Bank
Dr. Shankar Acharya (Non-Executive Chairman)
Uday Kotak (Executive Vice-Chairman & MD)
Lakshmi Vilas Bank
Parthasarathi Mukherjee (MD & CEO)
Nainital Bank
Mukesh Sharma (Cahirman & CEO)
Ratnakar Bank
Narayan Ramachandran (Non-Executive Chairman)
Vishwavir Ahuja (MD & CEO
South Indian Bank
Salim Gangadharan (Non-Executive Chairman
V. G Mathew (MD & CEO)
Tamilnadu Mercantile Bank
H. S. Upendra Kamath (MD & CEO)
Yes Bank Ltd.
Rana Kapoor (MD & CEO)
Ashok Chawla (Non-Executive Chairman)
Public Sector Banks and their CEOs & MDs
CEO / CMD / Head
Allahabad Bank
Usha Ananthasubramanian (MD & CEO)
Andhra Bank
Suresh N. Patel (MD & CEO)
Bank of Baroda
Ravi Venkatesan (Non-Executive Chairman)
P. S. Jayakumar (MD & CEO)
Bank of India
G. Padmanabhan (Non-Executive Chairman)
Dinabandhu Mohapatra (MD & CEO)
Bank of Maharashtra
Ravindra Prabhakar Marathe (MD & CEO)
Canara Bank
T N Manoharan (Chairman)
Rakesh Sharma (MD & CEO)
Central Bank of India
Rajeev Rishi (CMD)
Corporation Bank
Jai Kumar Garg (MD & CEO)
Dena Bank
Ashwani Kumar (CMD)
IDBI Bank Ltd
Mahesh Kumar Jain (MD & CEO)
Indian Bank
T C Venkat Subramanian (Non-Executive Chairman)
Kishore Piraji Kharat (MD & CEO)
Indian Overseas Bank
T C A Ranganathan (Non-Executive Chairman)
R. Subramaniakumar (MD & CEO)
Oriental Bank of Commerce
Animesh Chauhan (MD & CEO)
Punjab And Sind Bank
Jatinder Bir Singh (CMD)
Punjab National Bank
Sunil Mehta (MD & CEO)
Sunil Mehta (Non-Executive Chairman)
Syndicate Bank
Arun Shrivastava (MD & CEO)
State Banks of India
Arundhati Bhattacharya (Chairman)
UCO Bank
Ravi Krishan Takkar (MD & CEO)
Union Bank of India
Arun Tiwari (MD & CEO)
United Bank of India
Pawan Kumar Bajaj (MD & CEO)
Vijaya Bank
G Narayanan (Non-Executive Chairman)
Kishore Kumar Sansi (MD & CEO)
In Case if there is any correction in List of CEOs, MDs & Heads of Indian Banks 2017 [Updated] – SSC, Bank Exam, then please comment.
[table id=1 /]


text:
Libraries Transform Cookbooks
Libraries Transform Why libraries? Because cookbooks turn appetizers into canapés. Explore new worlds in your kitche... Read More
All information provided is deemed reliable but is not guaranteed and should be independently verified by the school's administration, as school boundaries are subject to change. If you need help finding a school in the current school boundary, please contact us.
No listings were found matching your search criteria.
[back to top]
About Longfellow Elementary School
Henry Wadsworth Longfellow Elementary School is run by the Pasco School District education agency. Longfellow Elementary is situated in the county of Franklin and the City of Pasco.
Longfellow Elementary School is categorized as a Primary School which offers Kindergarten to Fifth Grade. The approximate student population is 580 with 29 teachers. The student-teacher ratio is 20 students for every teacher. It is a Title I eligible school but not a charter nor a magnet school. Majority of the students are Hispanic and the rest are Caucasian, Indian, African American, and Asian. There are more male than female students.
The school is named after Henry Wadsworth Longfellow, an American poet of the 1800's, who wrote poems like Paul Revere's Ride, Evangeline, A Psalm of Life, The Song of Hiawatha, and Christmas Bells. He was also an educator and a linguist. Among the honors he received were honorary degrees from Oxford and Cambridge Universities, Queen Victoria's and the Prince of Wales' invitation and the Russian Academy of Sciences and the Spanish Academy chose him as a member.
Among the school's events are the Back to School Picnic, We Care Sports, Family Math Night, and Family Reading Night. The school encourages parents to join in their children's activities as parent involvement plays a big part in every student's success.
The school also has the Make Your Day Citizenship Program wherein the teachers are allowed to teach and students are allowed to learn. In this program students do what is expected from them to the best of their ability. This program teaches students that their learning behavior when they leave school until the following morning is their responsibility. It also motivates students in a positive way because of the feeling that they can succeed and they can be the best in whatever they choose to do.
The curriculum of Longfellow Elementary School is in line with the Essential Academic Learning Requirements or EALR. The EALR was created in 1933 by the Washington State Commission on Student Learning. EALR outlines expectations for each student's accomplishment in the core subject areas of Reading, Mathematics, Writing, Science, Arts, Communication, Health and Fitness, and Social Studies.
Longfellow Elementary School's mission is to provide students a secure learning environment, to teach using the best practices, and to encourage parent involvement to prepare them to become competent academically, respectful, good and contributing citizens of society.
There are several goals for school improvement. One goal is that every year until 2008, an increase by ten percent of the students meeting the WASL standards in the reading and mathematics portion. Another goal is a ten percent increase in parent involvement every year until 2008.
Henry Wadsworth Longfellow Elementary School, the home of the Longhorns, prioritizes the students and prepares them to become life-long learners.
|Pasco Wa Homes For Sale
|Under $100,000
|$100,000 - $125,000
|$125,000 - $150,000
|$150,000 - $175,000
|$175,000 - $200,000
|$200,000 - $250,000
|$250,000 - $300,000
|$300,000 - $350,000
|$350,000 - $400,000
|$400,000 - $450,000
|$450,000 - $500,000
|$500,000 - $600,000
|$600,000 - $700,000
|Over $700,000
|Condos & Townhomes For Sale
|New Homes & Construction
|Land & Acreage
Libraries Transform Why libraries? Because cookbooks turn appetizers into canapés. Explore new worlds in your kitche... Read More
BECAUSE YOU WANT MORE FOR YOUR CHILD Mid-Columbia Libraries offers Accelerated Reader (AR) books searchable by level, interest, ... Read More
Warm Up with Cool Books Dates: Wednesday, December 27, 2017 - 1:00pm to 4:00pm Thursday, ... Read More
Do you want your middle schooler reading more? Mid-Columbia Libraries offers Accelerated Reader (AR) books searchable by leve... Read More
Attend an open mic for all word lovers at the Paper Street Brewing Company for Words in Progress. Poetry, prose, memoirs, jou... Read More
Mid-Columbia Reads: Travelogue of Africa with Doris Mager | Mid-Columbia Reads celebrates Scottish author Alexander McCall Smith wi... Read More
In a time when Fall Carnivals are requiring entrance fees of $20ish, the Richland Public Library and Hapo Community Credit Uni... Read More
Tips and Tools for Smart Aging: Mid-Columbia Libraries' Kennewick Branch hosts the following workshops: Nutritious Meal ... Read More
Friends of Mid-Columbia Libraries hosts its fall Giant Book Sale Oct. 26-29 at the Kennewick library. Friends members get early acc... Read More
Dayton on Tour Downtown Dayton|Dayton, WA|Oct. 7, 2017 4 Historic Private Residences will be open to the public to ente... Read More


text:
The event hours are as follows:
Friday, May 18 @ 5-10pm
Saturday, May 19 @ 12-8pm
Sunday, May 20 @ 12-5pm
Our studio will be open all weekend in the Q.arma Bldg.
1224 Quincy St NE Studio B25
Minneapolis, MN 55413
Look for the Big A again. And be sure to stop by on Saturday afternoon and evening. There will be live bands, movies after sundown, a BBQ and drinks. We are sponsoring the Kotou Danse Company, who will also be performing at 4pm. Check out the tentative entertainment schedule.
Rabi will have about 5 new pieces at the Frank Stone Gallery.
1224 2nd St NE
Minneapolis, MN 55413
Preview Party: Thursday, May 17 @ 5-9pm (we will be there)
Group Show Reception: Friday, May 18 @ 7-10pm (we won't be there)
Open Gallery: Saturday and Sunday, May 19-20 @ 12-5pm
He will also have 4 pieces at Gallery 13.
302 13th Ave NE
Minneapolis, MN 55413
Along with other members of TAWU, Rabi will have 2 pieces at the Palm Court Restaurant.
2424 Central Ave NE
Minneapolis, MN 55418
Please support NEMAA at its silent auction and bid on his piece "Union". All proceeds from his piece go to NEMAA and help out the arts district.
Northrup King Bldg
1500 Jackson St NE Room #332
Minneapolis, MN 55413
We will post updates as they become available. Please let us know if you need directions to any of these venues or more information. Invite your friends!


text:
March 30, 2005
Inclusivity Action Plan addresses ethno-cultural challenges in York Region
NEWMARKET – The Human Services Planning Coalition (HSPC) has approved The Road to Inclusivity: An Action Plan For York Region. The report proposes a community plan to increase York Region's capacity to respond to the needs of immigrants and new Canadians.
The report is timely considering a recent Statistics Canada report detailed how immigrant newcomers to Canada are expected to represent more than one-fifth of Canadian residents by 2017. Weaving a New Canada cites the benefits that immigrants bring, along with the challenges associated with health-care access, language education, cultural transitions and job access.
"We simply cannot wait for the rest of Canada to catch up to us," said City of Vaughan Regional Councillor Joyce Frustaglio, Co-Chair of the HSPC. "Current funding models for human services are not fair to the taxpayers of York Region and especially to newcomers who need services and supports now, not in the future, when it's too late."
The anticipated impact of immigration on Canada over the next 12 years represents half the impact currently experienced in York Region. In 2001, immigrants made up 39 per cent of York Region's total population – a rate nearly double the one-fifth (or 20 per cent) proportion of immigrants anticipated in Canada by 2017.
"The impact of rapid growth in York Region is huge and human service providers are facing challenges to make York Region more ethno-culturally inclusive without the resources to address the needs," said HSPC Co-Chair Bill Hogarth. "We need fair and appropriate funding mechanisms now to respond to these real ethno-cultural challenges."
The challenge for service providers is to remove barriers to the full participation of immigrants and to tap into the rich experiences, skills and abilities of this growing population.
The Inclusivity Action Plan is the result of six month's of community consultations and an Inclusivity Summit earlier this year, attended by 180 people representing 95 different organizations in York Region.
The Road to Inclusivity: An Action Plan For York Region contains six specific actions:
• Development of a welcome centre for immigrants to help people become settled into appropriate employment and housing and to gain access to education, health care and social services
• Language development programs
• Region-wide education campaign to raise awareness of inclusivity practices
• Learning opportunities for children to advance inclusive and respectful character traits at an early age
• Volunteer opportunities for immigrants and the development of their leadership skills
• Tools to help organizations become more inclusive
Representatives from the organizations and ethno-cultural communities attending the summit have already formed working groups to develop recommendations for approval and implementation.
For more background on the report, including where people can obtain a copy, please visit [insert Web link here] or call Susan Taylor, Director of Human Services Planning Branch, York Region, at (905) 830-4444 or 1-877-464-9675, ext. 1514.
For more information on The Regional Municipality of York, the services we offer and links to our nine area municipalities, please visit our Web site at:
Contact: Tim Paleczny, Corporate Communications Services, York Region
(905) 830-4444 or 1-877-464-9675, ext. 1238
Back to top


text:
The circus is coming to Miss Ginny's Story Time on Friday, May 18, at 11:30 a.m. The circus-themed program will also include a fun craft with all materials provided. This will be the last story time until August.
Toddler Story Time and the Adult Book Club will continue through the summer. Play Day, Miss Ginny's Story Time and the Junior Book Club will break during the summer. The programs will return in August or September.
The Roots to Grow Tutoring is moving to Saturdays only. Demand for the program has dropped off as school comes to an end for this year.
June 4 is the first day for logging reading in the 2018 summer reading program "Reading Takes You Everywhere." This year, the program will go back to using the amount of time reading as the gage for awards. In 2017, the online program required the measure be books read. Librarian Sandra Brautigam stated parents observed their children were reading smaller less challenging books to complete the numbers. This was a major factor in returning to the time reading reward concept.
"The time reading measure is also more supportive for struggling readers. These children can gain the most from the benefits of reading through the summer. Reading books they choose takes reading from school work to fun," said Sandra. "In the process of reading for fun, they increase their skills through practice. Studies have shown children who participate in summer reading programs are more school ready and do better in mathematics than children who have not. Reading for pleasure also stays with children who later become life-long learner adults."
Awards for time reading will begin Monday, June 18. The grand prize drawing will be held on Friday, July 27. Prizes include bicycles and a promise of two one-day passes to Disneyland. The basic program has four levels. To encourage reading after the program time has been completed, children then earn extra reading tickets which apply to the larger prizes in three opportunity drawings.
The Canyon Lake Library is a collaboration with the City of Canyon Lake and the Riverside County Library System. It is located at 31516 Railroad Canyon Rd. Hours are Mondays from 10 a.m. to 7 p.m., Wednesdays and Fridays from 10 a.m. to 6 p.m. and Saturdays from 10 a.m. to 3 p.m. The library is closed on Tuesdays, Thursdays and Sundays. The Canyon Lake Library is a member of the Riverside County Library System and honors cards and returns from other affiliated libraries. For more information, call 951-244-9181.


text:
LGBTQ Pride
All Pride, No Prejudice
LGBTQ PRIDE, All Pride, No Prejudice
LGBT Pride Month: June 2017
Lesbian, Gay, Bisexual and Transgender Pride Month (LGBT) is observed in June to recognize the contributions of the lesbian, gay, bisexual and transgender community. It's celebrated in June to commemorate the anniversary of the 1969 Stonewall riots in Manhattan, New York City. Learn more about LGBT Pride Month from the Library of Congress.
Photo above: LA County Library staff marching in the 2018 Los Angeles Pride Parade.
LGBT Collection at the West Hollywood Library
Our West Hollywood Library holds our LGBT Collection.
Everyone is welcome to visit the second floor of the West Hollywood Library to browse this special collection, which includes classic and contemporary LGBT fiction and nonfiction.
The Lambda Literary Book Club meets monthly at the West Hollywood Library – call or visit for details.
Most materials can be sent to other LA County Library locations by placing holds.


text:
EASTON, Conn. -- A bacterial infection that can be prevented by vaccinations has infected dogs in Easton and Monroe, according to the Easton Courier.
Easton Animal Control officials are warning residents to get their dogs vaccinated for leptospirosis, also known as lepto, the Easton Courier reported. The disease can be spread through the urine of infected animals such as raccoons, with no direct contact needed. It can infect dogs while they are running around outside, as well as humans who get close to infected pets or walk around outside with bare feet.
The Easton Courier reported that one dog passed away from the disease, and there have been a number of cases reported in Easton and Monroe. The disease is treatable, but without treatment it can lead to kidney damage, liver failure, respiratory distress and death, the Easton Courier reported.
You can take steps to prevent leptospirosis by taking care of rodent problems and getting pets vaccinated, according to the Easton Courier.
Click here to read the Easton Courier story.
Click here to sign up for Daily Voice's free daily emails and news alerts.


text:
At least within our society, we adopt a way to measure lifes experiences. It is a simple, subjective way to measure: Good things feel good, and bad things feel bad. Thus, if something feels good, it cannot be bad; and if something feels bad, it cannot be good.
Most hasten to add that there are numerous exceptions: Discipline rarely feels good. Chemotherapy rarely feels good. Yet, both are administered and endured because an eventual improvement is sought, but not guaranteed.
My point is NOT that ones feelings have no value in determining wise and unwise, good and bad, or right and wrong. My point: the Christian who uses only his or her feelings as a primary means to determine (1) the meaning of a scripture, (2) right or wrong, or (3) correct behavior makes himself or herself an inviting target for Satans deception.
Before Jesus raised Lazarus, he told Martha, Your brother will rise again. Martha thought only of the final resurrection. When Jesus affirmed he was the power of life and resurrection, Martha did not feel an immediate solution to her grief for her brother. (See John 11:23,24.)
When Jesus was in Gethsemane less than 24 hours before his death (Matthew 26:38-44), or Paul was frustrated by his thorn in the flesh (2 Corinthians 12:7-10), or Peter was afraid of the Judaizing teachers (Galatians 2:11, 12), or Barnabas was deceived by hypocritical forces (Galatians 2:13), feelings either threatened to mask or did mask God at work. Jesus feelings said, Death is unacceptable. Pauls feelings said, You can be more effective for God without this thorn. Peters feelings said, I do not want to experience that again. Barnabas feelings said, They cannot be wrong about this.
Thank God Jesus did not surrender to his feelings! Thank God Paul listened to Christ when He explained! May we be warned by Peters fear and Barnabas deception! Yes, always consider your feelings, but never let your feelings become Gods voice. (If we do not consider our feelings, we will have no conscience.)
Jesus knew God was at work in his death. He saw beyond how he felt and felt grieved for those who caused his death (Luke 23:34). Stephen even saw God at work in his death (Acts 7:59, 60). That is the key for the Christianto see God working when we experience the distasteful. God often works through human pain and injustice.
Link to other Writings of David Chadwell


text:
Reduce Cholesterol By Using These Natural Tips Easily
Reduce Cholesterol- High cholesterol is known as hypercholesterolemia too. It has the possibility of increasing the danger of heart attack and other heart diseases. This can be cured by taking medicine on proper time and changing lifestyle. These basic changes can make life healthy and happy again. Cholesterol is a sterol which is a type of a fat. It is one of the 3 major classes of lipids which utilize the animal cells to contrast membranes. So, these cells are manufactured from animal cells, plants which do not manufacture the cholesterol.
Click here to read:- 8 Home Remedies to get rid of Dead Skin Cells from Body and Face
Reduce Weight
If you have an extra pound on the body even just a little bit then you can be a victim of high cholesterol and it is important for you to lose at least 10% of your weight to come out of this problem.
Start doing a walk or exercise on daily basis and also take proper diet. Avoid eating oily food, junk food, fried and saturated fat food for at least sometime till you do not lose your weight. Avoid high cholesterol food which is written above and add more fruits and vegetables in your diet. Drink 1-2 glass of lemonade with warm water every day. This will helps you in cutting weight and Reduce Cholesterol.
Do not avoid small things at home like picking up remote, glass, clothes etc. etc. Doing these things can make you active. Fix the time of sleep and wake up. Take proper sleep for 8 hours.
Read below-written tips to reduce the cholesterol easily and naturally.
Healthy Fat:- Saturated fat which can be found in dairy products and in red meat can increase your cholesterol and low the density of lipoprotein, which is a type of bad cholesterol. Eat only lean meat, mono-saturated fat products, and low-fat dairy products only.
No Trans Fat:- Oily food, fried food, bakery products contains a good amount of trans fat which misguided you by putting a label of 'trans fat-free'. Before buying must check the ingredient list which sticks on the box. It will show you clearly that boxed food had an amount of trans fat which partially contained hydrogenated oil in it.
Cholesterol in The Food:- If you are suffering from diabetes or heart disease than do not eat more than 300 milligram and less than 200miligram of cholesterol per day. Avoid eating whole milk products, organ meat, red meat, and egg yolks, these products are high in cholesterol. Drink skimmed milk and eat lean cur meat only. If you are vegetarian you can eat all types of fruits (avoid sweet fruits like mango) and vegetables (avoid potato). Drink fresh juices only (avoid packed chemicals based juices).
Omega 3 Fatty Acid Food:- Omega 3 fatty acids help in lower the bad cholesterol, fishes like mackerel, salmon and herring are a good sources of Omega 3 fatty acid. You can also have walnut, almonds (soaked almonds are better) and ground flax seeds in your diet.
Take more grains in the diet which helps in reducing cholesterol, you can eat whole grain bread, wheat flour, and brown rice. Take more fruits, vegetables and fresh juice only which are a good source of fiber which helps in reducing cholesterol level.
Click here to read:- 8 Causes, Prevention and Treatments of Pain While Walking
Exercise:- Walking, exercise and sports are the best way to get rid of bad cholesterol. It does not matter you are overweight or thin. Must do physical activities, it will make you active and energetic. Exercise improves the level of testosterone in the body which also help in reducing stress and anxiety. Exercise helps you to gain HDL i.e means High-Density Lipoprotein which is good cholesterol. Must do exercise for at least 45 minutes a day, I am personally spending 90-120 minutes per day in various physical activities. You can consult your doctor before start exercise if you are suffering from high cholesterol problem.
Do a walk for 1 KM after having dinner or can go for 15-20 minutes bicycle ride. Start with little things and rise with them slowly. It will help you to come out of cholesterol problem (which is one of the major causes of heart attack) and make you healthy.
By doing these above-written activities you can easily reduce or finish bad cholesterol in your body. Which helps you to live healthier and you can focus on your work and family and live a happy life.


text:
http://livecleanhealth.com/wp-content/uploads/2017/08/IMG_1996.jpg 750 979 Doug Ferner Jr http://livecleanhealth.com/wp-content/uploads/2016/12/liveclean-logo.png Doug Ferner Jr2018-04-27 15:47:182018-04-27 15:47:18What's in Your Deodorant?
What's in Your Deodorant?
What's in your deodorant? Do you know?
Take a listen as Doug Sr. discusses some of the potential dangers lurking in conventional deodorants and why Life Stinks' 100% clean, three-ingredient, all-natural deodorant is exactly what we've been waiting for!


text:
Recalibrating Conceptualizations of "Cultures of Peace": A Cross-National Study of Nonviolent Attitudes
Eddy, Matthew
MetadataShow full item record
Eddy, Matthew
This dissertation pursues three broad questions. First, what are the correlates of nonviolent attitudes around the world? Second, which nations exhibit characteristics of robust "Cultures of Peace"? Third, are there signs that history and collective memory shapes attitudes, i.e., do cultures "learn" from experiences of war, peace, or nonviolence? A multi-method approach sought to further our understandings of propensities for peace at both the national and individual levels. First, an analysis of nation-level Gallup World Poll data (N=136 nations) identifies correlates of nonviolent attitudes and advances a critique of the Global Peace Index (GPI), grounded in the observed disconnect between structural and attitudinal indicators of peace in many nations. The Gallup World Poll analysis suggests that many forces of modernization instill forms of "callous cruelty" while failing to cultivate pragmatic nonviolent attitudes. For example, poor nations and nations with recent successful nonviolent revolution are more likely to affirm that nonviolence "works" than wealthier nations ranking high in the GPI. Moreover, it is argued from Gallup data that the accumulation of "peace capital" is quite specific, with a frequent disconnect between forms of principled and pragmatic nonviolence. Second, survey data were collected from two "maximally different" cases, university students in the U.S. (N=403) and Costa Rica (N=312), which have starkly divergent structural and historical relationships to peace and militarism. Utilizing a new survey instrument, factor analyses helped to identify cross-national variations in respondent adherence to ideologies of violence and nonviolence: militarism, realism, just war, or nonviolence. The results show Costa Ricans were significantly more peaceful than U.S. respondents on 48 out of 52 items. Susceptibility to "elite cues" was tested in an experimental section. Tests revealed gaps in historical knowledge of nonviolence offering support for the theory that "ideology has no history." Finally, a cross-national sample of state-approved history textbooks from 8 nations (Germany, Norway, Ghana, Chile, El Salvador, Guatemala, Costa Rica, and the U.S.) were analyzed as outcomes of collective memory processes. The relative neglect of significant nonviolent revolutions and campaigns in the majority of these textbooks suggests formidable obstacles to the proliferation of nonviolent ideology around the world.


text:
Flat Belt Pulley
Order Directly from Shapeways below
Flat belt pulley. It measures 25.643 mm dia with a 7.938 mm width. In O scale this is a 48-1/2″ x 15″ pulley. The rim has the correct crown for this size pulley. The shaft hole is 1.588 mm/0.0625″ dia. The pulley was designed using data from "A treatise on belts and pulleys" by John Howard Cromwell pub. 1888
Here I have a couple mounted using custom (cast plaster) supports. There is another laying half-covered by dirt and rubble .. managed to ruin that one by leaving in cleaner to long .. makes for great scrap though.
Leave a Reply