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Making Sense of Affiliate Marketing
How to build a six figure blog without millions of visitors or selling your soul.
Enroll in CourseLearn how I went from $0 in affiliate income to over $50,000 per month.
Are you a blogger but unsure of how to make money online? Or, maybe you've been blogging for awhile but haven't had much luck with affiliate marketing.
Making Sense of Affiliate Marketing is a step-by-step guide to creating and making money from your own successful affiliate marketing strategy. This course explains all of the ins and outs, so you are never left wondering where to go next when creating and implementing your affiliate marketing strategy.
This course makes affiliate marketing achievable. You can see your blogging income grow from the beginning, and the information in this course will help you well into the future.
If you're not earning money through affiliate marketing, then you are leaving money on the table.
Bloggers and online influencers can all successfully promote products to their audience and earn an income, while being genuine and trustworthy.
I started my blog in August of 2011, at the age of 22, without any hopes of ever earning an income from it. I started it as a hobby - just a way to journal life and talk about my personal finance situation.
I decided to look into affiliate marketing after a few years of blogging. I had always skipped over it, because I thought my blog was too small to make any real money from affiliate marketing. Plus, I just didn't think it was possible to earn a living from affiliate marketing.
Me, being able to make money while I'm sleeping or on vacation?
I surely did not think that would be possible.
Then, I started applying different affiliate marketing strategies to my blog, and they were working. I saw my blogging income increase dramatically every single month.
Now, I wake up and cannot believe the amount of income I am earning through affiliate marketing, all while living a great life and being able to help my audience.
I now travel full-time with my husband and our two dogs, and I earn over $100,000 a month regularly from blogging.
Whether you are a brand new blogger or if you've been blogging for years, this course will help you with your affiliate marketing strategies.
I have talked to several bloggers about how much money they earn through affiliate income, and the answers still surprise me. There are bloggers out there with millions of visitors who aren't making affiliate income.
That doesn't have to be the case, though.
This step-by-step online course for bloggers teaches you how to make money through affiliate marketing.
I show you how to succeed at affiliate marketing, and you don't even need millions of page views or tons of followers to start making money.
And, even better, it actually works!
"I took Michelle's course at the very beginning of my blogging journey when I knew NOTHING about affiliate marketing. I had no idea how to find affiliate programs, create deep links, cloak links or how to strategically add links to posts and newsletters. Michelle's course answered all these questions with easy to digest lessons, worksheets, spreadsheets and a workbook. I had my affiliate marketing up-and-running in no time, and five years later, I make about $8,000-$9,000 per month with affiliate marketing. Her course was incredibly helpful and instrumental to me."
- Kristin Hanes, The Wayward Home
"Michelle is unbelievable. I had a decent amount of traffic, but struggled with using affiliates wisely. I used to get really overwhelmed with affiliates and would be disappointed when I didn't see results from the enormous amount of effort I was putting into it.
Michelle was able to quickly identify what needed to be changed and within one month, my income rose 40% and has since quadrupled only 4 months after working with her! My income was $6,469.50 before I talked to Michelle, immediately jumped to $9,362.64 the next month and is now $24,680.25 (four months later) and climbing!
Michelle is my go-to person for step by step affiliate income strategy."
- Rosemarie Groner, The Busy Budgeter
The step-by-step affiliate marketing strategy course that shows you how to increase your affiliate income and make more money blogging.
Here are screenshots from just a few of my affiliate accounts:
"This was the first course I ever took and I saw how much money Michelle was making on affiliate income, so I knew she was successful and that I would rather learn from someone successful than try to figure it out on my own. Michelle gave me the basic tools and strategies that I could immediately implement on my blog (even though I was just a beginner!).
The course was really good, extremely digestible for beginners, and there are certain strategies that I learned that I still think about today (over 5 years later!). There are some techniques in there, without giving anything away, that you would just never think of on your own but they make all the difference when it comes to marketing and making your links accessible.
I started my blog in September 2017 and by October 2017 I already made $300 from affiliate marketing techniques. Then in October 2018, one year later, I earned over $3400 in a month from affiliate marketing. And that was really just the beginning of my blogging journey honestly, and I was making so much money from affiliate marketing because of what Michelle taught in her course."
- Sophia Lee, By Sophia Lee
"Since taking Michelle's course 5 months ago, my monthly page view average has been 36,715 but my affiliate marketing income has grown from an average of $272.94 per month to $4,400.19 per month. That's more than 1,500% growth! Within just 5 quick months, I've made more than $21,000 directly from the affiliate marketing tips in this course…. I made more than $10,000 in just March alone! Low page views is not an excuse for low affiliate marketing income. You don't have to struggle to earn a few dollars a month. Invest in yourself & get educated to start building your business right away. Let Michelle show you how an expert does it!"
- Saira Perl, MomResource
Affiliate marketing isn't as easy as slapping links on your website. This course will teach you exactly what you need to do so that you can earn money through affiliate marketing EASILY.
Learn my proven affiliate marketing TODAY!
Bloggers NEED this course!
Many bloggers and online influencers skip over affiliate marketing. They either think it's too hard, that it's a waste of time, that they will turn followers away, or that their followers just won't be interested.
I recently asked various bloggers how much they were earning from affiliate marketing. The most common answer was somewhere around $500 or less.
Some even said $0.
This included several bloggers who receive 1,000,000 page views or more every month.
These bloggers all have the same problem. It's that they don't realize how much money they are leaving on the table, and many don't know how to approach affiliate marketing the correct way.
Perhaps you've tried affiliate marketing in the past, but nothing seemed to work.
Trust me, I know how you feel. When I started out blogging, I knew NOTHING about it.
I was afraid to try new things, and I was definitely afraid of affiliate marketing. I didn't want to scare my readers away or waste my time.
Now, I earn over $50,000 a month through affiliate marketing, and I'm sharing all of my best tips. I did the hard part and personally tested all of my methods, so I know they work.
Now, all you have to do is apply them to your blog!
"I started my blog and didn't even know what affiliate marketing meant. And....I didn't do much about it. I think my largest month was around $500 last year and then died down to sometimes only a few bucks per month. It wasn't my focus, but I didn't realize I was leaving money on the table, meaning I was losing money every month! I decided to enroll in Michelle's course, and I quickly noticed I didn't know a thing when it came to true Affiliate Marketing! No wonder it just 'wasn't working for me'...
Results from the first 60 days of taking the course:
I am still nowhere close to implementing everything I've learned [from the course], but....My August affiliate income was $1,792.26.
That's a $1,705 increase since I became a student of Making Sense of Affiliate Marketing. So, my investment in the class was worth it!"
- Chris Peach, MoneyPeach.com
This course will show you how to build a successful money-making blog, without millions of visitors or selling your soul.
In this course there are 6 modules, over 30 lessons, several worksheets, bonuses, an extremely helpful and exclusive Facebook group, and more. I go through everything that you need to know about affiliate marketing, such as:
- What affiliate marketing is and how it works
- Why affiliate marketing is great
- The exact steps I've taken to earn over $300,000 from a single blog post
- How to pick the right affiliate products to promote
- How to increase conversions
- How to build trust and not lose followers
- Required disclosures that you need to know about
- The one major tool you need for affiliate marketing
- Different strategies to use to promote your affiliate products
- How to use Pinterest to succeed at affiliate marketing (yes, this includes the newest Pinterest affiliate rules!)
And much, much more!
This course is anything and everything about affiliate marketing.
Making Sense of Affiliate Marketing is worth much more than $197, and this is the lowest price you'll ever see it listed for. Join now and learn how to master affiliate marketing.
"I have followed Michelle's blog for years. Her blog posts are incredibly informative and never disappoint. Making Sense of Affiliate Marketing is no different. The detailed strategies she shares in her course can help any blogger implement affiliate marketing. Within two days I received my first ever affiliate sale! From then I was hooked. The Mastermind alone is worth the cost of the course and the immediate access to an affiliate marketing expert is priceless! I highly recommend Making Sense of Affiliate Marketing."
- McKinzie Bean, Momsmakecents.com
"Making Sense of Affiliate Marketing was one of the first courses I took when I was completely new to blogging. I kept hearing astonishing success stories about bloggers making five to six figures per month with affiliate marketing, but I had no idea what it was or how it worked.
During that time, I only made a couple hundred dollars from Google AdSense, despite having traffic to my blog. After hearing about so many great results from others who had taken Making Sense of Affiliate Marketing, I asked Michelle if I was the right candidate for this course.
She was confident and informed me that taking her course could help boost my overall blogging income. She was right – taking her affiliate marketing course was a turning point for me.
As soon as I learned and implemented what she taught in her course, I made my first $500 in affiliate income for the month. As I implemented more strategies, my affiliate income grew to $5,000+ per month, and then to a full-time income of at least $10,000 per month, solely from affiliate marketing.
Today, affiliate marketing accounts for a healthy portion of my income, and I can't even put into words how thankful I am for investing in Michelle's course. It was the one course that helped me not only diversify my earnings but also increase them.
If you're a beginner or a struggling blogger aiming to take your earnings to the next level, then this course is a must-have. Making Sense of Affiliate Marketing has the potential to transform your life and financial future."
- Ling, Finsavvypanda.com
Your Instructor
My name is Michelle Schroeder-Gardner and I run the award-winning personal finance blog, Making Sense of Cents. I started blogging entirely as a hobby, and I have now earned over $5,000,000 blogging, with over $2,000,000 of that coming from affiliate marketing.
My writing and advice has been featured on sites such as CNBC, Time, Business Insider, Oprah, Huffington Post, Forbes, Cosmopolitan Magazine, Zillow, US News, Nasdaq, MSN, and more.
When I'm not blogging, you'll find me exploring via sailboat (we used to RV full-time!) with my husband and daughter. We sold our house in 2015 and have been traveling full-time since!
Course Curriculum
StartThe Amount Of Traffic You Need
StartHow To Determine What Your Readers Want
StartWorksheet - Brainstorm What Your Readers Want
StartHow To Find Affiliate Programs
Start80+ Affiliate Program Ideas For Different Niches
StartWorksheet - Affiliate Programs You Are Interested In
StartAffiliate Tracking Spreadsheets
Everyone loves bonuses, right? By purchasing Making Sense of Affiliate Marketing, you gain free access to these helpful bonuses.
Bonus #1: Access to the private Facebook Mastermind group ($250+ value!)
By purchasing this course, you will receive access to the Making Sense of Affiliate Marketing Facebook Mastermind group. This exclusive group will help you connect with others who have taken the course so that you can get feedback, ask questions, learn from others in your community, and offer help of your own.
Bonus #2: Free Group Coaching Sessions ($100+ value!)
This adds, even more, value to the course. I want to help you with your blog so that you can increase traffic, your income, and so that you can help your followers, so what's better than this? Two Saturdays a month, there will be a group coaching session in the private Mastermind group for students in the course. It will be called "Ask Michelle Saturday." This is your time to get feedback and support from me, and you can ask any questions related to your business, course material, your affiliate strategy, blog, and so on. I will answer every question in the group coaching session.
Bonus #3: How To Drive Thousands of Visitors To Your Blog With Pinterest
Lauren McManus is a well-known Pinterest expert from Create and Go as well as other successful websites and courses. I asked her to share her best Pinterest tips as a bonus for my course because I am a huge believer in Pinterest and how it can help you to improve your affiliate income. This strategy has allowed her to receive hundreds of thousands of visitors from Pinterest, significantly improving her income.
Bonus #4: How to Always Get Approved To An Affiliate Program
Justine has managed affiliate programs for companies such as Shopify and Freshbooks. She is able to give this course a fresh prospective - from that of an affiliate manager! This is an amazing bonus that will show you how to always get approved to an affiliate program. She has assessed thousands of applications over the years and there's a lot that goes into the yes/no.
Bonus #5: 9 Things You Must Do When Something Goes Viral
Every blogger and/or online influencer dreams that their brand, social media post, or blog post will go viral. However, your brand or post going viral doesn't mean your work is finished. It actually means your work is just beginning! You'll receive a PDF outlining exactly what to do and how to benefit from something going viral.
Bonus #6: How To Increase Your Page Views
You do not need millions of page views per month to make money blogging, but it is something you will want to increase if you hope to improve your income. Once you understand what your readers want, understand how to effectively reach out to companies for partnerships, and know how to charge the correct rate, you can make a good income online, regardless of the number of page views you receive. Increasing your pages view is necessary to make money blogging, but there are many different ways to grow that number. You'll receive a PDF outlining exactly what you must do in order to increase your page views.
Bonus #7: My Affiliate Products And Services
This is a worksheet that will help you keep track of the affiliate programs you are in. This way, you can easily reference it and include affiliate links in new social media and blog posts.
Bonus #8: The Perfect Affiliate Link Checklist
Use the worksheet "The Perfect Affiliate Link Checklist" to make sure your affiliate link is perfect. A perfect affiliate link will lead to higher click-through-rates and conversions.
Bonus #9: How to Maximize Your Reach, Impact, and Revenue with Facebook Ads
This amazing bonus will show you how to maximize your reach, your impact, and your revenue with Facebook Ads by Monica Louie. This is a great bonus for ALL bloggers. I've personally been coached by Monica and she is very knowledgeable, especially when it comes to helping businesses succeed on Facebook. She has worked on more than 100 Facebook ad campaigns, including several traffic campaigns with cost per click as low as $0.02 and conversion campaigns with cost per result as low as $0.35. Her online journey began in 2015 when she shared how her family paid off $120,000 of debt in two years on a single, middle-class income. She is amazing and I'm so glad she shared her expertise with us.
Bonus #10: How To Legally Protect Your Blog
Liz Stapleton is a lawyer and blogger. A licensed attorney since 2012, and a blogger since 2014, she has spoken at both virtual and in-person conferences on the importance of legally protecting your blog and business. One of the most popular questions I receive about blogging is how to legally protect your blog, and Liz goes over the exact steps you need to take.
"Having built a flexible, location independent business that generates massive revenue through affiliate sales, Michelle is a model for having it all. Not only do I follow along for tactical strategies to improve my own affiliate business model, but for inspiration from the fabulous adventure-filled lifestyle her thriving business supports."
- Stefanie O'Connell, Stefanieoconnell.com
Do you want a better work-life balance? What about FREEDOM?
Frequently Asked Questions
"When we first started blogging we had no real direction on the type of content we should be creating to actually make money. We thought we were creating quality content and were not being strategic with what the end goal was. What was the reason for the content we were creating? How do we actually monetize the traffic we are getting? This is when we came across Making Sense of Affiliate Marketing and instantly signed up knowing it would have a huge ROI.
Making Sense of Affiliate Marketing is a must-have for any new, beginner, and advanced blogger looking to start actually making income from their blog. The course is extremely well put together and easy to understand and implement as you go. It's been the go-to affiliate marketing course for bloggers for years and for good reason.
After taking Making Sense of Affiliate Marketing we were able to implement new monetization strategies and within a week make back the money we spent on the course. The course helped us have a long term strategy for monetizing our blog and helped us go from making a few hundred dollars per month to thousands of dollars every single month. I cannot recommend this course enough to all bloggers."
- Kelan Kline, TheSavvyCouple.com
If you love a product, why not get paid to promote it?
"I purchased Making Sense of Affiliate Marketing after I had been blogging for a few months. I had made a few affiliate sales already but I felt like I was still missing something. Making Sense of Affiliate Marketing contains so many practical and actionable tips that you can benefit from right away. Immediately after taking the course and implementing the strategies I learned, I was able to double my affiliate income in just one month."
- Dale, Blogging Her Way
Not sure if this course is for you?
Making Sense of Affiliate Marketing: A course for bloggers and online influencers is perfect for you if:
- You want to have a profitable blog.
- You want to improve your blogging income even further.
- You want to earn income while you sleep.
- You want to write a blog post and earn money from it for years down the line, with minimal work needed.
- You tried affiliate marketing but haven't had any luck.
- You want to spend more time with your loved ones and have a better work-life balance.
- You love blogging and want to be able to grow your blog, build trust with your audience, and help more people!


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Language of Page:
Understanding SC 3.1.1
3.1.1 Language of Page: The default human language of each Web page can be programmatically determined. (Level A)
Intent of this Success Criterion
The intent of this Success Criterion is to ensure that content developers provide information in the Web page that user agents need to present text and other linguistic content correctly. Both assistive technologies and conventional user agents can render text more accurately when the language of the Web page is identified. Screen readers can load the correct pronunciation rules. Visual browsers can display characters and scripts correctly. Media players can show captions correctly. As a result, users with disabilities will be better able to understand the content.
The default human language of the Web page is the default text-processing language as discussed in Internationalization Best Practices: Specifying Language in XHTML & HTML Content. When a Web page uses several languages, the default text-processing language is the language which is used most. (If several languages are used equally, the first language used should be chosen as the default human language.)
Note: For multilingual sites targeting Conformance Level A, the Working Group strongly encourages developers to follow Success Criterion 3.1.2 as well even though that is a Level AA Success Criterion.
Specific Benefits of Success Criterion 3.1.1:
This Success Criterion helps:
people who use screen readers or other technologies that convert text into synthetic speech;
people who find it difficult to read written material with fluency and accuracy, such as recognizing characters and alphabets or decoding words;
people with certain cognitive, language and learning disabilities who use text-to-speech software
people who rely on captions for synchronized media.
Examples of Success Criterion 3.1.1
Example 1. A Web page with content in two languages
A Web page produced in Germany and written in HTML includes content in both German and English, but most of the content is in German. The default human language is identified as German (de) by the lang attribute on the html element.
Related Resources
Resources are for information purposes only, no endorsement implied.
Techniques and Failures for Success Criterion 3.1.1 - Language of Page
Each numbered item in this section represents a technique or combination of techniques that the WCAG Working Group deems sufficient for meeting this Success Criterion. The techniques listed only satisfy the Success Criterion if all of the WCAG 2.0 conformance requirements have been met.
Sufficient Techniques
Identifying default human language(s) using one of the following techniques:
Additional Techniques (Advisory) for 3.1.1
Although not required for conformance, the following additional techniques should be considered in order to make content more accessible. Not all techniques can be used or would be effective in all situations.
SVR5: Specifying the default language in the HTTP header (SERVER)
using http or the Content-Language meta tag for metadata (future link)
Common Failures for SC 3.1.1
The following are common mistakes that are considered failures of Success Criterion 3.1.1 by the WCAG Working Group.
(No failures currently documented)
Key Terms
- Web page
a non-embedded resource obtained from a single URI using HTTP plus any other resources that are used in the rendering or intended to be rendered together with it by a user agent
Note 1: Although any "other resources" would be rendered together with the primary resource, they would not necessarily be rendered simultaneously with each other.
Note 2: For the purposes of conformance with these guidelines, a resource must be "non-embedded" within the scope of conformance to be considered a Web page.
Example 1: A Web resource including all embedded images and media.
Example 2: A Web mail program built using Asynchronous JavaScript and XML (AJAX). The program lives entirely at http://example.com/mail, but includes an inbox, a contacts area and a calendar. Links or buttons are provided that cause the inbox, contacts, or calendar to display, but do not change the URI of the page as a whole.
Example 3: A customizable portal site, where users can choose content to display from a set of different content modules.
Example 4: When you enter "http://shopping.example.com/" in your browser, you enter a movie-like interactive shopping environment where you visually move around in a store dragging products off of the shelves around you and into a visual shopping cart in front of you. Clicking on a product causes it to be demonstrated with a specification sheet floating alongside. This might be a single-page Web site or just one page within a Web site.
- human language
language that is spoken, written or signed (through visual or tactile means) to communicate with humans
Note: See also sign language.
- programmatically determined (programmatically determinable)
determined by software from author-supplied data provided in a way that different user agents, including assistive technologies, can extract and present this information to users in different modalities
Example 1: Determined in a markup language from elements and attributes that are accessed directly by commonly available assistive technology.
Example 2: Determined from technology-specific data structures in a non-markup language and exposed to assistive technology via an accessibility API that is supported by commonly available assistive technology.

subset: commoncrawl


text:
FIND A CHAPTER > Borough of Manhattan Community College
Year Founded
Total Members
Proud NSLS Alumni
Nominated by Borough of Manhattan Community College? Enter your nomination code below.
Don't have a nomination code? Click here
BMCC NSLS Advisor
Gary Tuerack - Founder, NSLS
Learn the skills necessary to lead in your community and in the competitive job market. Through our skillbuilding leadership development program, NSLS members turn dreams into action.
Leadership Development Hours
Of members were happy with their chapter leadership
Of members thought their NSLS chapter should continue
Of members are satisfied with their NSLS membership
NSLS has been great in helping me recognize a lot of things about myself in terms of my leadership style. One of the most beneficial parts of the induction process was the Success Networking Teams because they set the foundation for a project that I have been looking forward to starting."
I loved learning from the speakers. I loved the orientation and the collection of people to learn from all in one place. I learned that although you may fail, there are ways to change your perspective and grow and become who you want to be. It is really inspirational."
After transferring to Dean College and taking my first steps to induction, achieving my goal of making President's List was a valuable accolade that I can say was a product of the leadership training process. The training the National Society of Leadership and Success offers tested my limits and continues to teach me ways to positively influence the lives of others."
The NSLS is more than just an organization. It is a life boost, career boost, and an emotional boost. I definitely recommend the NSLS to anyone in search of an organization that will benefit and support their vision."
November 30, 2023
Khodr Farhat has been appointed by Michigan Governor Gretchen Whitmer to the Council for Rehabilitation Services.
November 24, 2023
In November, we are so thankful to see NSLS members making headlines for living our mission and leveling up as leaders. See the top...
November 10, 2023
We're raising our freshly-brewed cups of coffee to Evan Perlinger, who has accepted an offer from Starbucks as a full-time software engineer.
No. The NSLS is not a scam. The NSLS is a legitimate, accredited, and esteemed leadership honor society that is nationally recognized, with over 800 chapters and more than two million members at universities nationwide.
Yes, the NSLS is a real, legitimate honor society. As a leadership honor society, our program and curriculum are focused specifically on leadership skill development and career preparation.
As a member, you have access to a wide range of training resources, networking events, and learning opportunities designed to develop your leadership skills. In order to become a fully-inducted member, students must complete the Steps to Induction.
Members usually spend less than 10 hours total at their own pace as they work toward completing the Steps to Induction. You can choose your level of involvement in the program. To get the most out of your experience, you'll want to be as involved as possible.
Members must meet certain nomination criteria that are specified by local chapter leaders (usually based on GPA and/or leadership potential). Once you have paid your one-time registration fee, you're considered a pre-inducted member. In order to become an inducted member, you must complete the Steps to Induction listed in the previous question.
Students who wish to join but haven't been nominated can still apply for membership online.
Throughout your college career, you may be invited to a number of different honor societies. Sadly, in some situations, scammers may pose as fraudulent honor societies to take advantage of college students. It's important to know how to distinguish between a scam and an honor society that will provide value to you.
Look for the below criteria when determining whether or not an honor society is legitimate:
Student success stories. A legitimate honor society will have testimonials and member success stories readily available. These stories should tell you how the organization helped members prepare for—and succeed— in their endeavors and the value it brought to their college experience.
Reports of the NSLS being a scam are largely due to the fact that there are very real honor society scammers out there who take advantage of college students. The NSLS isn't one of them. The NSLS partners with schools to bring leadership development to their campuses.
You can search through our chapter database to see more about the chapter you were invited to and our history with your school. So far, the NSLS has helped over two million members at more than 800 schools achieve success.
The fee to join the NSLS helps us continually develop our curriculum and deliver new and engaging content to our members. Without a fee, we wouldn't be able to reach as many future leaders as we do. To that end, we know that not everyone can afford the fee and have provided options to assist members who may be experiencing financial difficulties.
Sigma Alpha Pi (ΣAπ) are the Greek letters that the NSLS uses to represent itself. We are not affiliated with campus Greek life. These letters stand for Success, Action, and Purpose, which are the guiding principles of the NSLS.
For more information, please read our extended FREQUENTLY ASKED QUESTIONS guide.
Yes, the NSLS is a real, legitimate honor society. As a leadership honor society, our program and curriculum are focused specifically on leadership skill development and career preparation.
Accept your nomination to join the active NSLS community at your school and across the country. Learn the skills to prepare for your future and set you apart in the competitive job market. And, gain access to over $400,000 in scholarships.
Select the state in which your school is located
4300 Biscayne Blvd
Suite 203
Miami, FL 33137


text:
Ansell AERMLED3/60/DL Endurance TP(A) And UGR19
- Edge lit LED panel suitable for education, commercial and ancillary area applications
- UGR<19 (unified glare rating) compliant luminaire assisting to eliminate glare for visual comfort
- Cool white, warm white and daylight options
- White anodised aluminium frame for visual compatibility with ceiling grid
- Low glare and even light distribution for multiple applications
- TPa as standard
- Surface mounting frame option
- Powered by Tridonic
£40.44Excl tax £33.70
Ansell AERMLED3/60/DL Endurance TP(A) And UGR19
19 600×600 White 30W LED 3000lm 6000K IP44 Edge Lit Panel
Key Features
Edgelit LED panel suitable for education, commercial and ancillary area applications
UGR<19 (unified glare rating) compliant luminaire assisting to eliminate glare for visual comfort
Cool white, warm white and daylight options
White anodised aluminium frame for visual compatibility with ceiling grid
Low glare and even light distribution for multiple applications
TPa as standard
Surface mounting frame option
Powered by Tridonic
Useful Links
Brand | Ansell |
Colour | |
Colour Temperature | Daylight White |
Wattage | 30 Watt |
Size | 600 x 600 |
Product | LED Panel Light |
PIR | No |
Photocell | No |
There are no reviews yet.
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We always endeavour to have your order with you as soon as possible. Some of our items are ordered from our suppliers upon receipt of your order. Lead times vary depending upon the item and supplier, but we will always try to ensure delivery is made within 5 working days.
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To notify us of any faults or problems with your order please do so by visiting our 'Contact Us' page or via email to: email@example.com.
If you wish to return your order from Juice Electrical, or there is a problem, please notify us immediately. Before returning an item to us, you must provide us with all necessary information stating why you wish to do so. To notify us of any faults or problems with your order please do so by visiting our 'Contact Us' page.
Subject to the Consumer Protection (Distance Selling) Regulations 2000, Juice Electrical Supplies Ltd does not sell products on a trial basis. Please ensure suitability prior to buying.
In accordance with the Consumer Protection (Distance Selling) Regulations 2000, and providing the goods are unopened, unused and in a resalable condition, you may return your order within 14 days of receipt for a full refund of the product price excluding P&P. Please ensure you notify our returns team by emailing firstname.lastname@example.com prior to the return so we can issue a returns number.
All returns are fully inspected once they arrive in our warehouse and, if any product is returned in an unsaleable condition, this includes the item's external packaging, Juice reserves the right to reject any refund or apply a handling charge. We will, however, discuss this with you before taking any action.
Please ensure that you return any goods in an outer box or packaging as they will not be resaleable with handwriting, scuffs or labels stuck on the actual product box.
Your payment will be refunded as soon as the returned product has been processed.
You will only be reimbursed for the delivery costs if the product is faulty, or was a delivery error made by Juice Electrical Supplies Ltd.
If an item is thought to be faulty and no fault is found, the customer is responsible for the cost of return carriage.
Please be aware that any photographs used on the site are supplied by each supplier and are taken under professional lighting conditions. We cannot be responsible for the cost of return for items with a difference of likeness in colour, shade or appearance.
Due to palletised deliveries/collections and supplier restocking fees, any returns or exchanges of radiators may incur additional charges. Please email email@example.com for more information.
We cannot accept the return of any radiators that have been removed from the packaging.


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In 2047, Tiberium has blanketed the Earth, which is now divided into zones based on the level of Tiberium infestation. Pristine Blue Zones represent 20% of the planet's surface and are the last refuge of the civilized world. Partially habitable Yellow Zones make up 50% of the planet's surface; most of the world's population lives in these war-torn and ecologically ravaged areas. The remaining 30% of the planet is uninhabitable, a Tiberium wasteland swept by violent Ion Storms. Red Zones are like the surface of an Alien planet.
The infamous Kane has returned to lead the Brotherhood of Nod in a massive global assault on the Global Defense Initiative and the few remaining Blue zones left on the planet. Only you can stop him. The fate of humanity – and the planet – is in your hands.
Featuring state-of-the-art, next-generation graphics, an epic story with live action, full-motion video sequences, and the classic fast-paced C&C style of play, C&C 3 sets the standard for the single player RTS experience.
When you decide to test your skills online, a suite of truly innovative features, such as the ability to transform online battles into a spectator sport, unfold.
Welcome back, Commander.
Key Features
The ultra-responsive, smooth-as-silk gameplay C&C invented is now better than ever, intuitively placing your entire arsenal at your fingertips.
Over 30 Single Player Missions
In a vast open-world 'theatre of war' where each decision you make matters, you'll experience one cohesive, epic story told from the unique perspective of all three warring factions.
RTS as a Sport
Observe, broadcast, and compete in thrilling online battles with innovations including all-new interactive spectator modes, VoIP communication, player commentary, and more.
Story Like You've Never Seen
High-definition, live action video sequences that seamlessly tie the game's epic story together featuring an unparalleled, diverse cast.
Adaptive AI
The game AI matches your style of play - choose from distinct AI playing styles such as 'rushing', 'turtling', and more, or level the battlefield and let the AI use its own tactics to give you the highest level of challenge.
New Ways to Attack
Keep your opponent guessing by building flying battleships, wielding the power of terrifying Ion Storms, and combining units to form powerful never-before-seen super-units.
Lose yourself in reflected sunlight and scream in sheer joy as you blow chunks off of enemy tanks, structures, and anything else you can launch a rocket at. The game world gains a new dimension and the battlefield comes to life as the atmosphere fills with smoke, fire, haze, tracers, explosions, and state-of-the-art weather effects.
Intuitive and Evolved Interface
Fully evolved side-bar interface that allows you to fully manage your base operations while never leaving the action.
Battle the Best Online
With multiple online game modes, all-new multiplayer maps, and a fully featured content editor, go online to see how you stack up in RTS' ultimate forum.


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This is a perfect example of Christmas giving. Start a legacy of holiday gifting this year! It's just a small, white envelope stuck among the branches of our Christmas tree. No name, no identification, no inscription. It has peeked through the branches of our tree for the past ten years or so. It all began because my husband Mike hated Christmas. Oh, not the true meaning of Christmas, but the commercial aspects of it: overspending, the frantic running around at the last minute to get a tie for Uncle Harry and the dusting powder for Grandma, the gifts given in desperation because you couldn't think of anything else. Knowing he felt this way, I decided one year to bypass the usual shirts, sweaters, ties and so forth. I reached for something special just for Mike. The inspiration came in an unusual way. Our son Kevin, who was 12 that year, was wrestling at the junior level at the school he attended, and shortly before Christmas, there was a non-league match against a team sponsored by an inner-city church. These youngsters, dressed in sneakers so ragged that shoestrings seemed to be the only thing holding them together, presented a sharp contrast to our boys in their spiffy blue and gold uniforms and sparkling new wrestling shoes. As the match began, I was alarmed to see that the other team was wrestling without headgear. It was a luxury the ragtag team obviously could not afford. Well, we ended up walloping them. We took every weight class. And as each of their boys got up from the mat, he swaggered around in his tatters with false bravado, a kind of street pride that couldn't acknowledge defeat. Mike, seated beside me, shook his head sadly, "I wish just one of them could have won," he said. "They have a lot of potential, but losing like this could take the heart right out of them." Mike loved kids, all kids, and he knew them, having coached little league football, baseball and lacrosse. That's when the idea for his present came. That afternoon, I went to a local sporting goods store and bought an assortment of wrestling headgear and shoes and sent them anonymously to the inner-city church. On Christmas Eve, I placed the envelope on the tree, the note inside telling Mike what I had done and that this was his gift from me. His smile was the brightest thing about Christmas that year and in succeeding years. For each Christmas, I followed the tradition: one year sending a group of mentally handicapped youngsters to a hockey game, another year a check to a pair of elderly brothers whose home had burned to the ground the week before Christmas, and on and on. The envelope became the highlight of our Christmas. It was always the last thing opened on Christmas morning and our children, ignoring their new toys, would stand with wide-eyed anticipation as their dad lifted the envelope from the tree to reveal its contents. As the children grew, the toys gave way to more practical presents, but the envelope never lost its allure. The story doesn't end there. You see, we lost Mike last year to cancer. When Christmas rolled around, I was still so wrapped in grief that I barely got the tree up. But Christmas Eve found me placing an envelope on the tree, and in the morning, it was joined by three more. Each of our children, unbeknownst to the others, had placed an envelope on the tree for their dad. The tradition has grown and someday will expand even further with our grandchildren standing to take down the envelope. Mike's spirit, like the Christmas spirit, will always be with us. Comments are closed.
August 2020


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Hello! Happy Wednesday everyone!
Today is the day that we announce our Top 3 designs from Sketch # 487. How exciting! All of our designs this week were fabulous and we love them all. Our Top 4, while all are awesome, they are shown in no particular order. We have 4 today as there was a 3 way tie in voting for our second most favourite card, so rather than revote we are honouring our top 4 today!
Congratulations on your great designs ladies! Please feel free to grab out "Top 3" blog badge from the right side bar and display it proudly. For even more great designs, jump on back and check out all of the great entries to Sketch # 487 here.
Wednesday is a fun day, not only do we announce our Top 3 but we also get to bring you the fun and creative creations from our Design Team for Sketch #488.


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69 Lees Crossing Rd, Valley Falls, NY 12185This property at 69 Lees Crossing Rd, Valley Falls, NY with bedrooms and 0.00 acres is not currently listed for sale on NY State MLS. Only NY State MLS participants and their agents may list properties for sale on the NY State MLS. If you believe real estate property in the city of Valley Falls at 69 Lees Crossing Rd should be listed for sale and is not, please contact us.
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Avapro is used for treating high blood pressure alone or with other medicines. It is also used in certain patients to treat kidney problems caused by diabetes (diabetic nephropathy). Avapro is an angiotensin II receptor blocker (ARB). It works by relaxing blood vessels. This helps to lower blood pressure.
Use Avapro as directed by your doctor.
- Take Avapro by mouth with or without food.
- Take Avapro on a regular schedule to get the most benefit from it. Taking Avapro at the same time each day will help you remember to take it.
- Continue to take Avapro even if you feel well. Do not miss any dose.
- If you miss a dose of Avapro, take it as soon as possible. If it is almost time for your next dose, skip the missed dose and go back to your regular dosing schedule. Do not take 2 doses at once.
Ask your health care provider any questions you may have about how to use Avapro.
Store Avapro at 77 degrees F (25 degrees C). Brief storage at room temperatures between 59 and 86 degrees F (15 and 30 degrees C) is permitted. Store away from heat, moisture, and light. Keep Avapro out of the reach of children and away from pets.
Active Ingredient: Irbesartan.


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About AAA
AAA serves more than 57 million members. Your local club is available to serve you through branch office locations and online services. Take full advantage of every AAA membership benefit including a variety of services that can help you save money.
Your AAA membership is a membership for life. Use it every day to get discounts and deals at places to shop around town from restaurant deals, hotel deals, movie tickets, and more. Use AAA on your smartphone to find cheap gas nearby and get travel information, AAA Diamond rated hotels, restaurants, attractions, events, and more. In times when you need auto repair, roadside service or a new car battery, a AAA tow truck or service vehicle will be on its way to help.
When it comes to travel, AAA gets your trip heading in the right direction. Partnerships with Royal Caribbean and Carnival Cruise Lines, among others, make for some sweet cruise deals. Work with a AAA travel agent or use online trip planning tools to find cheap hotels, book flights, reserve rental cars and even find the best motel along your road trip. Use your AAA membership to get hotel discounts, air discounts and car rental discounts. Also, check out the best AAA Diamond rated hotels and restaurants.
Travel Information
Whether you're planning a family trip to Universal Studios Orlando or a Las Vegas getaway, use AAA travel information to be a well-informed traveler. Travel Guides provide detailed destination information and travel tips, Drive Trips can help you plan vacations to go, and TripTik maps will help you find nearby restaurants, gas stations and last minute hotels.
Savings & Discounts
If you want to know how to save money, AAA membership is one answer. With discounts at more than 100,000 places across North America, you're sure to save whether you're at home or traveling. With a AAA membership, you can save at places where you may already shop, dine, and visit, including attractions and events. AAA offers local discounts near you, and national discounts that provide ways to save money.
Auto Services
As a AAA member, you have peace of mind when things on the road don't go as planned. When you need Roadside Assistance or a new car battery, a AAA tow truck or service vehicle is just a tap, click, or call away. If you're looking for a mechanic you can trust, use AAA's network of Approved Auto Repair shops. (AAR shops). And when it's time for a replacement, use AAA's comprehensive car buying resources for new cars and used cars.
Turn to AAA for your insurance needs. Your AAA insurance agent can help you get the right home insurance or car insurance policy to help protect you and your family.
Financial Services
When it comes to peace of mind, AAA has your finances covered too. Learn more about ID protection services, credit cards, and a selection of financial products for domestic trips as well as international travel. AAA also offers currency exchange to ensure you're prepared to enjoy traveling abroad.


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Palouse residents can get the best smart home technology in the nation, with Vivint
Smart devices are supposed to make modern life easier, but when you have three or more different smart home toys that are all controlled through different apps, you really just have one giant headache on your hands. Switch to the service that makes life easier and install a Vivint system in your home today to centralize control over all your smart home conveniences and safety features. The basis of any good smart home system is centralized control. If you have to log in to various accounts on different apps just to control your home's alarm system and convenient smart devices, that's not really a 'smart' system. What sets our smart home systems apart from other providers? Our proprietary Vivint Smart Drive offers a layer of intelligence that goes far beyond basic programmability. You may already manually program some appliances and devices in your home, but Vivint takes it a leap further by bringing all of your devices together into a connected ecosystem. Take control of your system by optimizing your settings via your touchscreen panel, Vivint app or by integrating Amazon Echo into your system. We offer a simple, intuitive navigation path that makes it easy to manage your home from anywhere in the world with an internet connection.


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Enjoy complimentary express worldwide shipping on all orders over $1,000 AUD
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Complimentary Worldwide Express Shipping for all orders over $1000 AUD. Find Out More
A new classic, the Ember Diamond Bracelet represents the NMJ alchemy of tradition and innovation – timeless materials rendered in a contemporary silhouette.
All payments on nataliemariejewellery.com are processed via our secure payment gateway.
We also offer buy now, pay later plans via PayPal, Afterpay or Zip Pay. Please find out more specifications below.
We accept Visa, MasterCard and American Express. No transaction limit. Learn more about Payments here.
Repay in 4 instalments fortnightly, interest-free. The maximum purchase amount is $2,000 AUD. Learn more about Afterpay here.
Choose weekly, fortnightly or monthly repayments. The maximum purchase amount is $5,000 AUD. Learn more about Zip Pay here.
Repay in 4 instalments fortnightly, interest-free. The maximum purchase amount is $2,000 AUD. Learn more about PayPal Pay In 4 here.
Yellow Gold
A classic and timeless metal, yellow gold is revered for its rich, vibrant hue. Courtesy of its warm and luxurious appearance, many cultures historically imagined gold to represent the sun, and its durability makes it a preferred material for ceremonial pieces.
70 per cent of the gold that we use in our creations is recycled Australian gold, with just 30 per cent newly mined. Of the latter figure, 86 per cent is confirmed as Australian mined, and the remaining 14 per cent is blended. We harness this blended portion predominantly for our clasps, earring backs and findings.
Rose Gold
An alloy of gold and copper, Rose Gold is recognisable for its warm, peach-toned hue that complements a vast range of skin tones and designs. An uniquely coloured metal, existing somewhere between white gold's stark brilliance and yellow gold's classicism, Rose Gold is a romantic option for engagement and ceremonial styles.
The Rose Gold used for our creations is 70 per cent is recycled Australian gold, with 30 per cent newly mined. Of the latter, 86 per cent is confirmed Australian mined, the remaining being blended (used for clasps, earring backs, findings etc).
White Gold
With its bright, silvery-white, lustrous surface, White Gold is a cherished metal for jewellery and other keepsakes. Created by combining pure gold with other metals, its modern and sophisticated expression wears beautifully across different skin tones.
70 per cent of the gold that we use is recycled Australian gold, with 30 per cent newly mined. Our White Gold is rhodium plated – an industry-standard process whereby the natural colour of the metal is plated in a thin layer of rhodium (a naturally white metal), to evoke a luxurious hue that is consistent no matter the carat, and is reapplied annually.
Without delving too deep into the science of metals and alloys, our bespoke team have explained the differences to help you make the best choice for your next NMJ piece, and also shared with us whether there is a noticeable difference in colour between the carats.
The difference between 9ct, 14ct and 18ct gold is the precious metal content. 9ct gold contains 37.5% pure gold, 14ct contains 58.5% pure gold and 18ct gold contains 75% pure gold. The remainder is a mix of various metals, depending on whether it is a yellow, rose or white gold piece.
In general, 18ct gold is the longer lasting, more durable choice, however 9ct gold can still be a great option for some Jewellery pieces. 9ct gold is commonly used for fine pieces of jewellery that are typically designed for casual wear. Our seasonal collection fashion pieces, or statement pieces are typically crafted in 9ct gold. The reason for this is that these are generally our finest pieces, and where 18ct is flexible, 9ct gold is more rigid and holds its shape better as it has the lowest pure gold content – making it great for casual wear while also bringing it into a more accessible price point. At NMJ, we always recommend 18ct gold for all precious pieces such as engagement rings and wedding bands.
Put simply, the higher the gold content, the richer the colour and warmer the tone, particularly for yellow and rose gold. However, only when side-by-side are these differences really noticeable to the eye, and they are generally quite subtle so don't affect the overall aesthetic of your piece of jewellery. The thinner or smaller your piece, the less noticeable the colour difference will be.
As an industry standard, most white gold is rhodium plated. This means you're not actually seeing the natural colour of the metal as it is plated in a thin layer of rhodium, which is a naturally white metal, so that the stunning bright silver-colour appears consistent no matter the carat. This finish is generally reapplied annually when rings are serviced to maintain the colour.
If you're opting for an un-plated white gold, the natural colour of the metal in 9ct is slightly darker than sterling silver. You will find that the 9ct looks silvery, and then the higher the carat the darker the grey tone becomes, resulting in more of a steely grey with a slight warmth, making it a popular choice for mens wedding rings.
9ct rose gold is a soft pink, while 14ct and 18ct are a much warmer, peach-toned colour. Our 18 carat rose gold alloy is always popular for its soft warmth and lack of coppery undertone.
9ct yellow gold appears as a subtle, pale yellow while higher carats, such as 18ct in particular, present a richer, more vibrant golden tone. As a 9 carat alloy is more diluted with other metals, there can be some minor colour evolution or tarnishing over time, whereas 18 carat has a more stable finish.
The carat finish can be identified by a small stamp (hallmark) located on the gold, typically in a discreet spot such as the inside of a band, the clasp of a necklace or bracelet or the post or butterfly of your earrings.
9ct - '9ct', '9k' or '375'
14ct – '14ct', '14k' or '585'
18ct – '18ct', '18k' or '750'
As all our pieces at Natalie Marie are handmade to order, we can customise your piece to suit your exact size (unless noted otherwise). We offer two standard bracelet or bangle sizes and where possible, we ask you to measure the bracelet size that will suit you perfectly. Simply, with a piece of string, mimic the length of a bracelet (including clasp) and measure this in cm. Select the bracelet length that closely corresponds with the string, or contact our team for a custom bracelet size.
Crafted for you | Estimated dispatch 4-6 weeks
Handmade in 14ct White Gold. Stone: An array of round brilliant cut diamonds, size dependent approx. total carat weight: 0.62ct. Our small diamonds are graded DEF colour, VS-SI1 clarity as standard. Chain: Handmade link components. Length: 16cm or 17.5cm, please select your preference.
This piece is handmade to order. Please allow approximately 4-6 weeks for the production of this piece.If the piece has optional customisation offerings, please refer to the estimated dispatch time presented by the add to cart button as this may affect the lead time.
SHIPPINGWe ship globally, using express priority shipping methods to ensure traceability and insurance, and offer complimentary worldwide shipping on all orders over $1,000 AUD.Duties and taxes levied by the recipient country are the responsibility of the customer. For more details on shipping and delivery here.
INSURANCEWe partner with Centrestone Jewellery Insurance. We recommend learning more about their service and considering insurance on your treasured pieces. For more details, contact our team here.
NMJ pieces are mindfully handcrafted, predominantly in our in house Sydney studio. We pride ourselves on creating not only a safe and healthy working environment for our team of jewellers, but also a nurturing and supportive platform for their growth as jewellers and professionals in their field.
We encourage and support skill development and personal growth with a focus on creating a positive and enjoyable workspace. Using hand tools as opposed to heavy machinery lessens our carbon footprint, while selecting non toxic compounds for use in the studio reduces our environmental impact.
We have recently partnered with accredited facilities both in Sydney and overseas to facilitate the expansion of our production to meet a growing demand, while we work on building out our own team to facilitate this in house. You can read more about our manufacturing avenues here.
We'd love to answer any questions you have in regard to this piece. Simply use the form below with any questions you may have.
Curated for you
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It is of the utmost importance to Natalie Marie Jewellery to create responsibly, source mindfully and ensure that all of our pieces are produced sustainably and ethically.
We donate 1% of total online sales to IMPACT to help to support the incredible work they do across Regulatory and Legal Reform, Supply Chain Transparency, Gender Equality, Illicit Trade and Financing and Environmental Stewardship.
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On the same day cyclist Bradley Wiggins became Great Britain's most-decorated Olympian, Daniel Harris, a 28-year-old cycling enthusiast, was killed when he was struck by a bus at an intersection outside Olympic Park.
Wiggins, who also this summer won the Tour De France and had a starring role in the Olympic opening ceremony, commented upon Harris' death and said something that would have, to many in the United States, been seen as occupying a space somewhere between sensible and obvious.
When asked if he favored mandatory helmet laws for cyclists, Wiggins responded that he did, adding, "because ultimately, if you get knocked off and you ain't got a helmet on, then how can you kind of argue?" He went on to say, "when there's laws passed for cyclists, then you're protected and you can say, 'well, I've done everything to be safe.' "
Wiggins was denounced for his remarks.
Cyclists and non-cyclists; conservatives and liberals — they all united in arguing that wearing a cycling helmet should be a matter of choice, or else the popularity of cycling might decline. Darren Johnson a London Assembly member from the Green Party, said the issue of mandatory helmet laws missed the point. "We need to focus on the solutions to the problem of left-turning lorries," he said.
David Cameron, the Conservative Prime Minister who can be seen here, here, and here cycling without a helmet, called compulsory laws a "difficult issue," though he hasn't backed them in the past.
The leading liberal newspaper in London, The Guardian, opined against mandatory helmet laws and conducted an online poll in which 79 percent of respondents said bike helmets should not be mandatory. The Rupert Murdoch-owned Times of London also opined against helmet laws, taking care to point out that the pro-bicycling campaign they champion does not call for mandatory laws.
The heart of the "pro-choice" argument here in Great Britain is that mandatory laws will put people off cycling, which would have a much worse effect than however many lives are saved by helmets. There is a study reliably cited in all anti-mandatory helmet law arguments, which indicates that drivers give more berth to helmetless bike riders. Also cited is the example of Australia, where mandatory helmet laws actually do seem to have made cycling less popular, possibly without the added benefit of protecting many Australian heads.
And while responsible English parents do, by-and-large, seem to make sure their little ones are helmeted, it is a far-from-ubiquitous practice.
Dr. Mark Porter, a top official with the British Medical Association, has said, "My children refuse to wear helmets. I would prefer them to ride without than not cycle at all." An American pediatrician who routinely expressed such thoughts might find himself in want of patients. In the U.S., 21 states, including 4 of the 5 most populous, have mandatory helmet laws for minors. The majority of riders do wear helmets.
From what I've seen personally, in New York City, where I live, it is uncommon to see a rider other than a food delivery guy zip by helmetless. Here in London, it seems that a great many bike riders don't wear helmets.
The explanation might be that the discussion is focusing on the wrong things. Mandatory helmet laws in U.S. states are largely uncontroversial, because Americans already subscribe to notions of helmet efficacy.
Social norms theory — the notion that most people behave based on notions of their peers' behavior — explains why this is so. Clever politicians have used social norms theory to expand use of carpool lanes and dog leashes. Think about handicapped parking spaces. The able-bodied rarely park in them, not because they fear a fine — but because they find such an action abhorrent.
Whether they're put off by their own sense of ethics or fear of the disapproval of others is beside the point; the behavior has been established as a norm. If in England, the wearing of bike helmet were to be a norm — if a mum who allowed her little ones to bike without helmets were shunned by her peers in the PTA — not only would mandatory helmet laws become easier to pass, they'd become beside the point.
Copyright 2021 NPR. To see more, visit https://www.npr.org.


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Learn Romanian: Extremely Handy Tips [2024]!
How to learn Romanian language online for free? Romanian is spoken by around 25 million people worldwide, primarily in Romania
How to learn Romanian language online for free? Romanian is spoken by around 25 million people worldwide, primarily in Romania
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Current Position:Home>>Antibodies>>Polyclonal Antibody>> SERPINA5 Polyclonal Antibody
Lead Time: Order now, ship in 3 days
Welcome to order from local distributors.
Add to cart Bulk requestReactivity | Application |
human |
liver cancer,A549, )
Western Blot analysis of Human liver cancer and A549 cells using SERPINA5 Polyclonal Antibody at dilution of 1:750. Western Blot analysis of Human liver cancer and A549 cells using SERPINA5 Polyclonal Antibody at dilution of 1:750. (
lung cancer, )
Immunohistochemistry of paraffin-embedded Human lung cancer using SERPINA5 Polyclonal Antibody at dilution of 1:40. |
1.Protein extraction
1)For tissue sample
a. Take the samples, wash the tissue thoroughly with pre-cooled PBS (0.01 M, pH=7.4)(Cat# E-BC-R187) to remove the surface blood and internal debris.
b. Weigh and smash the tissue, add an appropriate ratio of RIPA Lysis Buffer (Cat# E-BC-R327)(add 10 μL PMSF and 10 μL Na3VO4 to each 1 mL RIPA Lysis) and homogenizely lyse the tissue.
It is recommended to homogenize according to the ratio of tissue weight: RIPA volume = 3:10. For example, add 1 mL RIPA Lysis Buffer to 0.3 g tissue sample, the specific volume can be adjusted according to experimental requirements.
c. Shake and lyse on the ice for 30 min after homogenization. And then sonicate the sample for 1 min (under ice water bath conditions) with 2 s' sonication and 2 s' intervals to make cells fully lysis and reduce the viscosity of sample.
d. Centrifuge at 12,000 rpm for 10 min at 4℃.
e. Take the supernatant and measure the protein concentration mentioned in step2.
2)For cell sample
a. Collect the cells, wash them thoroughly with pre-cooled PBS (0.01 M, pH=7.4) to remove the medium off (it is generally recommended to wash 3 times).
b. Add an appropriate ratio of RIPA Lysate Buffer (10 μL PMSF and 10 μL Na3VO4 in each 1 mL RIPA Lysis) and lyse on the ice for 30 min.
It is recommended to add 0.1 mL of RIPA Lysis Buffer to each well of a 6-well plates (the protein content in different cells may vary, and the volume of the lysate added can be appropriately adjusted).
c. Sonicate the sample for 1 min (under ice water bath conditions) with 2 s' sonication and 2 s' intervals to make cells fully lyse and reduce viscosity of sample.
d. Centrifuge at 12,000 rpm for 10 min at 4℃.
e. Take the supernatant and measure the protein concentration mentioned in step2.
2.Measurement of protein concentration
By the BCA method (see the Total Protein Colorimetric Assay Kit (Cat# E-BC-K318) instructions).
3.Boiling the samples
Adjust the protein concentration with PBS Buffer. Add 5 × SDS Loading Buffer (Cat# E-BC-R288) with the ratio of the protein sample: 5 × SDS Loading Buffer = 4:1 and boil the mixture for 10 min. Centrifuge at 12,000 rpm for 2 min and collect the supernatant. The denatured protein can be employed to Western Blot experiments or stored at -20℃ or -80℃.
Note: It is recommended that the total protein loading amount of test sample is about 50 μg in each well. Try to make the loading volume of each sample close to 10 μL.
1.According to the molecular weight of the target protein, prepare 0% separation gel. Add the test sample to each well, and add 5 μL of Pre-stained Protein Marker (Cat# E-BC-R273)to a reserved well in order to verify the target molecular weight and the extent of membrane transfer. Add Electrophoresis Buffer ( Cat# E-BC-R331) and start electrophoresis.
2.Electrophoresis at 80v when the samples are in stacking gel, then convert to 120v when the blue flow into the separating gel. Electrophoresis time is about 2-3 h till bromophenol blue reaches the bottom of the gel.
1.Choose the PVDF Membrane (Cat# E-BC-R266) with a pore size of μm according to the molecular weight of the target protein. Soak the PVDF Membrane in methanol for 1 min to activate it, and then soak the PVDF Membrane in the Transmembrane Buffer (Cat# E-BC-R333), the filter paper and fiber mat must be soaked in the Transmembrane Buffer for use too.
2.Follow manufacture instructions of Transfer System for wet, semi-dry, or dry transfer.
1.Soak the PVDF Membrane with TBST Buffer (Cat# E-BC-R335) containing 5% Skim Milk Powder as blocking buffer and block the membrane at room temperature for .
2.According to the recommended primary antibody dilution ratio, use the TBST Buffer containing 5% Skim Milk Powder to dilute the SERPINA5 Antibody at , soak the PVDF Membrane in the primary antibody working solution, incubate overnight at 4 ℃, and gently shake.
3.Wash the PVDF Membrane with TBST Buffer for .
4.According to the recommended secondary antibody dilution ratio, use a TBST Buffer solution containing 2% Skim Milk Powder to dilute Goat Anti-Rabbit IgG (H+L) (peroxidase/HRP conjugated) (Cat# E-AB-1003) at . Incubate at room temperature for 1 h on a shaker.
5.Wash the PVDF Membrane with TBST Buffer for .
1.Mix A and B in the Excellent Chemiluminescent Substrate Detection kit (Cat# E-BC-R347) at the ratio of 1:1 as working solution.
2.Take out the PVDF Membrane from TBST Buffer and absorb the liquid with the filter paper. Pave the PVDF Membrane on the detection machine, add ECL working solution continuously on the PVDF Membrane, discharge the bubble and detect the result.
3.Adjust the contrast and the exposure time to get the best image.
Clonality | Polyclonal |
Isotype | IgG |
Concentration | 0.3 mg/mL |
Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer, Cell Biology, Cardiovascular |
Conjugation | Unconjugated |
Immunogen | Recombinant protein of human SERPINA5 |
Abbre | SERPINA5 |
Synonyms | Acrosomal serine protease inhibitor,IPSP,IPSP,PAI 3,PAI-3,PAI3,PCI,PCI-B,PLANH 3,PLANH3,Plasma serine protease inhibitor,Plasminogen activator inhibitor 3,Plasminogen activator inhibitor III,PROCI,Protein C inhibitor,Serpin A5,Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 5,Serpin peptidase inhibitor clade A member 5,Serpina5 |
Swissprot | P05154 |
Gene Accession | BC008915 |
Calculated MW | 46kDa |
Cellular Localization | Secreted. |
Tissue Specificity | Expressed by the liver and secreted in plasma. |
Application | Products |
WB | Western Blot Detection Kit(E-IR-R304) |
IHC | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)(E-IR-R217) |
The protein encoded by this gene is a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. This gene is one in a cluster of serpin genes located on the q arm of chromosome 14. This family member is a glycoprotein that can inhibit several serine proteases, including protein C and various plasminogen activators and kallikreins, and it thus plays diverse roles in hemostasis and thrombosis in multiple organs. |
E***eSubmitted [ May 14 2019 ]
Asked: Pleasw what method does this use? We bought it in November but received only the buffer. How do we use it? No wells or strips included
adminSubmitted [ May 14 2019 ]
Answered: We provide only Antibody in the package which is anti-target protein antibody, no buffer was provide in this series product. This product is only SERPINA5 Polyclonal Antibody which can use in WB and IHC detection. Do you mean that you suppose to by the SERPINA5 ELISA KIT?


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text: Ron Barnes + Add to Google Calendar + iCal / Outlook export Date Oct 05 2023 Expired! Time 12:30 pm - 4:30 pm Share this event Recent Posts Who is Alcantara Vineyard and Winery and how do you grow grapes in the desert? Recent CommentsArchives December 2017 Categories Arizona Wine Country Running a Vineyard


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California has effectively become a one-party state. Election Day 2012 earned Democrats a two-thirds majority in both houses of the state legislature, the first time in 80 years that any party has achieved that feat in California. That will allow them to pass virtually anything they want in the next legislative session, including crafting ballot initiatives or passing laws to raise taxes that have previously been opposed by Republicans. But the GOP's newfound insignificance in this state legislature isn't its greatest worry. Republicans are warning that if the national party doesn't heed the example of California, it could head toward political irrelevance on a larger scale.
(MORE: The Winners and Losers of Election 2012)
That's because it was the California GOP's alienation of minority — especially Latino — voters starting decades ago that planted the seeds for its losses this month. Indeed, an important factor in Mitt Romney's defeat was lopsided Latino support for President Obama. "California Republicans are becoming the white man's party," says Allan Hoffenblum, a former manager of local GOP campaigns in California who now publishes the California Target Book, an almanac on the state's election campaigns. "Now I see the same thing happening on the national level. If the Republican Party doesn't reform itself and become a viable party again, something else is going to replace it."
There was a time when California was Republican. The state voted red in six straight presidential elections until Bill Clinton's victory in 1992. Since then, it has voted solidly Democrat for President. Experts say this decline can be partly traced back to former Republican governor Pete Wilson's endorsement of Proposition 187, a 1994 ballot initiative that aimed to deny undocumented immigrants access to public services. "Prop 187 really inflamed the very nasty polarizing debate over immigration and the hard rhetoric of the conservative right," says Sherry Bebitch Jeffe, senior fellow at the USC school of policy, planning and development. The measure passed, but a federal court ultimately found it unconstitutional. More important, the state's Hispanics found the proposition appalling and rallied their communities to reject it and the party that endorsed it.
(MORE: The Tarnished State of California's Golden College System)
"These people were angry," says Arturo Vargas, who at the time worked at the Mexican American Legal Defense and Educational Fund. Since then, the GOP's attitude toward Latinos hasn't softened, and Hispanics' allegiance to Democrats hasn't either. Meanwhile, the Latino population ballooned with an influx of immigrants, only adding to the ranks of Democratic voters. Over time, districts that were traditionally red turned blue, and there were more areas where GOP candidates couldn't be competitive. "History has shown that that moment galvanized Latino immigrants," says Vargas, who is now executive director of the National Association of Latino Elected and Appointed Officials. "That's why California is so blue today."
Blue is an understatement. Not only did Democrats win a supermajority but Obama and the state's Democratic candidate for U.S. Senate both got landslide victories, voters approved a key tax increase opposed by Republicans, and GOP voter registration dipped below 30%, which is not much greater than the percentage of voters with no party preference. Governor Jerry Brown too is a Democrat and could be re-elected if he decides to run again in two years.
(MORE: Jerry Brown's Risky Crusade — How to Raise Taxes in California)
California Republican Party Chairman Tom Del Beccaro recognizes the state's changing demographics are a factor in these losses. While much more measured in his comments, even Del Beccaro says the GOP's inability to effectively engage minority voters in the state is a "warning." "Republicans haven't developed a relationship with Latino voters throughout the state," he says in an interview. "They haven't been aggressive enough talking to Asian voters, Latino voters, black voters and young voters."
Del Beccaro, though, doesn't believe it's the end of the road for California's Republican Party. Democrats' current policies as well as new legislation they may pass with their supermajority — such as tax increases and heavy regulations on business — could make the economy worse in two years than it is now, which would prompt more voter discontent with Democrats, he says. "Republicans need to start working today, going into the districts, explaining that those policies are hurting jobs and developing relationships with voters so that two to four years from now, voters will consider them as an alternative to Democrats," he says. In an editorial on Monday, the Los Angeles Times urged Democrats not to "go crazy" with new tax legislation. "Democrats, please. Slow down. Exercise some restraint," the editorial read. "Please don't crash, lest you take California down with you."
Still, Republicans in other parts of the country are seeing California as a potential bad omen. Jorge Luis Lopez, a Miami Republican who has led fundraising efforts for multiple campaigns and volunteered at the grassroots level for Romney, said Republicans are "doomed" in Florida and on the national level if they follow the path of the harsh rhetoric that hurt California's GOP. In fact, a poll by Miami's Bendixen & Amandi International showed Obama nearly pulled off majority support from Cuban Americans, whose support for the GOP used to be a given. "If Republicans want to engage the Latino community, we have to be able to get past the initial question of how compassionately we're going to address the immigration issue in America," Lopez says.
(MORE: Obama's California Bullet Train Is Still on Track)
Indeed, the big question is whether Republicans are willing to work with Democrats to achieve comprehensive immigration reform. While recent overtures by House Speaker John Boehner suggest his party may be ready to do so, pure legislation may not be enough. Years of harsh rhetoric — made worse by Romney's calls for self-deportation during the primaries — has led some Latinos to think the GOP regards them as inferior, says Dan Schnur, director of the Jesse M. Unruh Institute of Politics at USC. And that needs to be mended if the GOP is to right the ship. As Schnur says, "A Latino business owner isn't going to want to hear what a candidate has to say about the capital gains tax until he's convinced that politician doesn't consider him and his family to be something less than human."


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This work of genius is by Joel Bradshaw, a talented Wreckie with mad video editing skilz and even madder taste in blogs. Thanks for making my week, Joel! When our bid for Wrecky World Dominion is complete, you'll be first on the list for promotion to head henchperson. ;)


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Welcome to Ottawa, the beautiful and historic capital of Canada that is booming with new condo developments.
Sitting on the South Bank of the Ottawa River in southern Ontario, Ottawa borders Gatineau, Quebec, and forms the center of the National Capital Region. It has a population of over 1.3 million people and a history dating back to 1826. It's famously home to many cultural landmarks and educational institutions.
As well as being a cultural and educational hub, Ottawa is also home to countless condos. There are 428 condo buildings in Ottawa, and you can find a wide range of Ottawa condo buildings like Low-Rise, Mid-Rise, High-Rise, Luxury, Pre-Construction, and Lofts too. In short, there are many types of Ottawa condos for sale, offering something for everyone.
A big selling point of Ottawa is its diversity, and it has lots of interesting neighborhoods to explore and discover.
You can find a wide range of Ottawa condos in some of the city's most popular and rapidly-developing neighborhoods, including Centretown, Downtown Ottawa, Sandy Hill, Westboro, Hintonburg, Mechanicsville, Byward Market, The Glebe, and Lebreton. Centretown, Downtown Ottawa, and Byward Market tend to have the most condos in Ottawa.
Centretown, Downtown Ottawa, and Byward Market all have a high number of condos in Ottawa. These neighborhoods are also some of the best places to live in Ottawa, home to famous landmarks like the Peace Tower, the Canadian Museum of Nature, and ByWard Market Square.
The city also boasts many other exciting neighborhoods with Ottawa condos for sale, including neighborhoods that are quieter and calmer, ideal for growing families, and many of the best condo buildings offering easy access to Ottawa's top sights like Parliament Hill, the Rideau Canal, the National Gallery of Canada, and the Canadian War Museum.
The average days on market for condos for sale in Ottawa is 40 days, and the average sale price of condos in Ottawa is $390,239. There were 2039 Ottawa condos sold last year. Ottawa saw a decrease in the number of condos sold of -5% compared to the previous year. There are also plenty of new constructions of condos in Ottawa too, opening up more opportunities for new arrivals to this fabulous city.
In terms of general lifestyle for a condo in Ottawa, the city has a walk score of 7, a bike score of 25, and a transit score of 26. Ottawa condo dwellers can rely on the city's OC Transpo public transport system of buses and light rail transit to get around with ease, and the capital region has more than 300km of bike paths too, ideal for cyclists.
Become a Condo Dork
1025 RICHMOND Road, Ottawa, K2B 8G8, ON is offered for rent. The asking price is 2400.00 and it has been on the market since 5/30/2023. Featuring 2 bedrooms and 2 bathroom. The Continental and Ambleside 3 are nearby condo buildings.
Woodroffe North offers a lifestyle full of green space for outdoor lovers, parks include Ambleside Park, New Orchard Park, and Deschenes Rapids.