text:
October 13, 2009
Phylogeny of mtDNA haplogroup M in India (Chandrasekar et al. 2009)
Updating Phylogeny of Mitochondrial DNA Macrohaplogroup M in India: Dispersal of Modern Human in South Asian Corridor
Adimoolam Chandrasekar et al.
To construct maternal phylogeny and prehistoric dispersals of modern human being in the Indian sub continent, a diverse subset of 641 complete mitochondrial DNA (mtDNA) genomes belonging to macrohaplogroup M was chosen from a total collection of 2,783 control-region sequences, sampled from 26 selected tribal populations of India. On the basis of complete mtDNA sequencing, we identified 12 new haplogroups - M53 to M64; redefined/ascertained and characterized haplogroups M2, M3, M4, M5, M6, M8′C′Z, M9, M10, M11, M12-G, D, M18, M30, M33, M35, M37, M38, M39, M40, M41, M43, M45 and M49, which were previously described by control and/or coding-region polymorphisms. Our results indicate that the mtDNA lineages reported in the present study (except East Asian lineages M8′C′Z, M9, M10, M11, M12-G, D ) are restricted to Indian region.The deep rooted lineages of macrohaplogroup 'M' suggest in-situ origin of these haplogroups in India. Most of these deep rooting lineages are represented by multiple ethnic/linguist groups of India. Hierarchical analysis of molecular variation (AMOVA) shows substantial subdivisions among the tribes of India (FST = 0.16164). The current Indian mtDNA gene pool was shaped by the initial settlers and was galvanized by minor events of gene flow from the east and west to the restricted zones. Northeast Indian mtDNA pool harbors region specific lineages, other Indian lineages and East Asian lineages. We also suggest the establishment of an East Asian gene in North East India through admixture rather than replacement.
50 comments:
- terryt said...
So now we have 64 M lineages, of which just a few managed to seep outside India, presumably very slowly. So much for India being a major Paleolithic migration route.
- Wednesday, October 14, 2009 2:20:00 am
- eurologist said...
That just shows M is ancient in India, and most subsequent migrations happened early.
- Wednesday, October 14, 2009 6:24:00 am
This is a good study. I don't consider the origin point of the "Ms" in situ to be too significant. I doubt any of the female carriers of the "Ms" were like any modern humans today, and unlike any living Indians today. Mitochondrial haplogroup M and N are ancient, and the subcontinent of India, a part of Gondwanaland that slammed into the Eurasian continent, is a landmass conducive to human population growth, so India containing many subclades of mtDNA M is understandable. The migration route of AMHs out of Africa was via south India, and it proceeded to SE Asia, Australia, and then north to the east Asian coastlands. The subcontinent was not left unoccupied but acted as a conduit to further migration which has persisted into the modern era and influenced the cultures and religions of mainland SE Asian countries and islands.
The East Asian admixture, if that is what it is called considering it is ancient and probably precedes the formation of the East Asian racial groups, is really a no brainer conclusion. STP studies of Indians shows an East Asian or mongoloid inheritance of many of the northern South Asians. - Wednesday, October 14, 2009 12:14:00 pm
- Gioiello said...
We are racist for our theory. Indians are altruistic in demonstrating that mtDNA M is born in India and deep rooted there from at least 50ky.
But by demonstrating this, they demonstrate also that hg. R has nothing to do with ancient India. India, like America, is the result of Indian women and "European" men. - Wednesday, October 14, 2009 2:54:00 pm
- German Dziebel said...
The principal investigator, Adimoolam Chandrasekar, is the supporter of the back migration of YAP+ Y-DNA from Asia into Africa. See his "YAP insertion in South Asia" (2007). He's looking for the same signal in mtDNA. The established migration of some U and M lineages into North and East Africa doesn't match the magnitude and the antiquity of the YAP+ back migration. So Chandrasekar simply formulates the problem (which is an old problem dating back to at least Cann et al. 1987) as the lack of ancient M lineages in Africa and the lack of L3 lineages in India.
- Wednesday, October 14, 2009 5:00:00 pm
- Maju said...
I'm still reading the paper, so no much to say yet on it. However, Gioello wrote:
But by demonstrating this, they demonstrate also that hg. R has nothing to do with ancient India. India, like America, is the result of Indian women and "European" men.
What does this rant mean if anything at all? Are you talking of mtDNA R (highest top level diversity in India very clearly) or Y-DNA R (more complex maybe but also with apparent older phylogeny in India)? Whatever the case you are probably at least partly wrong.
India (or rather South Asia) is a very large subcontinent with very old phylogenies. It is most likely that all or at least most European (and West Eurasian in general) lineages are one way or another derived from Indian ones. Guess that Y-DNA is less clearly cut, specially for IJ (and of course E), but there is virtually no argument to make regarding mtDNA in this aspect. - Thursday, October 15, 2009 8:09:00 am
- Maju said...
So now we have 64 M lineages, of which just a few managed to seep outside India, presumably very slowly. So much for India being a major Paleolithic migration route.
The paper argues that "East Asian" lineages M8 (that includes CZ), M11 and M12'G in India "are rather ancient with ages >50,000 years". These lineages are AFAIK also old in East Asia.
So much for your preconceived pseudo-scepticism. - Thursday, October 15, 2009 8:16:00 am
- Maju said...
From the paper:
It is apparent that all the ancient lineages under analysis emerge directly from the root of the macrohaplogroup M.
This is an important confirmation. As I have mentioned before, very few haplogroups at any phylogenetic level, approach such an immense starlike structure of more than 40 top-level sublineages. Only H does but not even this Western clade has so many top-level subclades (31 "only"). Then come, at quite some distance other macro-haplogroups like N and R (12 and 16 top tier sublineages respectively). For comparison L3 only has 7.
It is my understanding that this structure implies a rather sudden and dramatic demographic expansion as which can only happen when a population expands in virtually virgin, essentially uninhabited, land, from a small number. This M starlike structure is the most clear signal of the Eurasian demic expansion that happened after soon after the OOA (or maybe, if we like it better catastrophist, after the Toba supervolcano caused a massive bottleneck among early Eurasians). Of course the comparatively smaller N and R starlike structures do also represent that same expansion in one way of another. However the starlike structure of mtDNA H must represent some other similar event but circunscribed to West Eurasia, such as the colonization of Europe some 45-45,000 years ago (and there was also a supervolcano then, for the catastrophists). - Thursday, October 15, 2009 8:39:00 am
- Maju said...
From the paper, for TerryT in particular:
The North Asian route could not get support from mtDNA due to the lack of basal M, R, N lineages in northern Asians, thereby ruling out the existence of a northern Asian route. And follows a large list of reference footnotes. - Thursday, October 15, 2009 8:47:00 am
- Gioiello said...
Maju, I have no time to answer you deeply. My reasoning was simple: if mtDNA M is deeply rooted in India (50kyBP), if YDNA R has been originated in India, we should have mtDNA M everywhere we have YDNA R. This isn't, then YDNA R has nothing to do with INDIA. Is it clear, or not?
- Thursday, October 15, 2009 3:22:00 pm
- German Dziebel said...
I don't know, guys. I still see it as a "slippery slope" model: a few archaic lineages C/Z, D, M9-M12 entered India from the east when populations were still small (because they're found all the way into the Americas, M included), then a cornucopia of new ones emerged in situ as a result of rapid population expansion.
L3 is not found in India, hence it's probably never been there. And this study also confirms that there're no archaic M lineages in Africa. - Thursday, October 15, 2009 5:20:00 pm
- Maju said...
My reasoning was simple: if mtDNA M is deeply rooted in India (50kyBP), if YDNA R has been originated in India, we should have mtDNA M everywhere we have YDNA R.
Why? South Asia also seems ancestral for other lineages, notably mtDNA R, which is relevant in the sense you mean, as almost all West Eurasian mtDNA is R. Similarly, South Asia also hosts other Y-DNA lineages like H, L or C5, which have not seen any significative expansion out of India.
Of West Eurasian mtDNA lineages all but maybe X and M1 have a marked relation with South Asia (sister lineage/s only found there) and can be argued to have originated in the subcontinent. This is very clear for the case of mtDNA R.
L3 is not found in India, hence it's probably never been there. And this study also confirms that there're no archaic M lineages in Africa.
L3 is in India and everywhere, in the derived form of M or N. However if you mean L3(xM,N), you are right. Though I don't know why it would matter, as some sort of "bottleneck" or rather founder effect is always considered when dealing with OOA (otherwise Eurasians would have all the African diversity and we don't). Still, 2/7 L3 subslineages are Eurasian, what means that the bottleneck was not so severe: just a rather wide founder effect.
In the long term you won't find L3-underived anywhere, the same you don't find M-underived, etc. All lineages evolve one way or another. Our Indian and otherwise Eurasian L3 is necessarily in the form of M and N, unless there is a third Eurasian lineage not found till now. If it makes you happier, just call M and N with their more meaningful names of L3m and L3n, ok? - Thursday, October 15, 2009 7:29:00 pm
- Maju said...
Oh, man, that last comment was not from some ignorant incidental poster but German Dziebel, who is obsessed with his own theory and with not understanding genetics: he is not ignorant, he wants to remain that way.
Would I have noticed, I would not have replied because it's a waste of time and virtual ink. I think you are confusing people with your false ignorance: you know very well all that by now. - Thursday, October 15, 2009 7:37:00 pm
- German Dziebel said...
Luis, no L lineages are found outside of Africa. Not L0, not L1, not L2, not L3. Nowhere. No M and N lineages are indigenous to Africa. This is not a founder effect or a bottleneck. It means that the current mtDNA phylogeny is wrong. Just do me a favor, reverse a few mutations and show me what happens.
"L3 is in India and everywhere, in the derived form of M or N."
I responded to this nonsense a few times already. According to you (and you owe this thought to some awkward phrasing found in certain papers), the whole world is Africa. You can build circular arguments but they're not convincing. - Thursday, October 15, 2009 8:34:00 pm
- terryt said...
"The North Asian route could not get support from mtDNA due to the lack of basal M, R, N lineages in northern Asians, thereby ruling out the existence of a northern Asian route".
The lack of basal lineages in no way rules a northern Asia route. As you yourself later say, 'In the long term you won't find L3-underived anywhere, the same you don't find M-underived, etc. All lineages evolve one way or another'. So why would you expect basal lineages to survive? especially as we can expect selection, bottlenecks and drift to have been severe along such a route. And It's possible the authors are a little committed to the idea that India has been important in our origin story?
Leaving out M and R, which I'll concede are southern, we do actually find some very early N lineages through Central Asia: A, X and, in the Far East, Y/N9.
"South Asia also seems ancestral for other lineages, notably mtDNA R".
Why? I see Ian Logan still has R on the Y/N9 line, so it need not be Indian in origin. He also has New Guinea P as being close to R's origin. It's quite possible that R is SE Asian in origin. - Friday, October 16, 2009 6:30:00 am
- Gioiello said...
If mtDNA R is very ancient, also Tuscany has it. A relative of mine, I tested by SMGF, is resulted R0a, the same haplogroup that the last paper on Etruscans (Arredi et alii if I remember well) found they haven't in common with Middle Easterners and the authors probably too hastily thought that what was common was from there (I can demonstrate that it could be the contrary too).
See: Mitosearch W9E9S. - Friday, October 16, 2009 8:44:00 am
- Gioiello said...
Of course the paper is "Alessandro Achilli et alii, Mitochondrial DNA Variation of Modern Tuscans Supports the Near Eastern Origin of Etruscans".
- Friday, October 16, 2009 9:51:00 am
- Gioiello said...
The European R0a with no mutations in HVR2 (Mitosaerch WQYNW and QZQJ6) and the basic mutations of R0a in HVRI (126C, 362C), is probably the European R0a closer to CRS and probably European mtDNA H arose from a common European haplotype.
That Tuscany retains the descendants of these ancient haplotype demonstrates, I think, that probabbly Italy was the source, remembering also the probably mtDNA H of Paglicci (28kyBP). - Friday, October 16, 2009 11:24:00 am
- Maju said...
I think we're all the time talking CRS as in HVS-I, not the full sequence. HVS-2 does not seem to be relevant as is almost never tested.
- Friday, October 16, 2009 4:51:00 pm
- German Dziebel said...
Apropos Terry and Luis: I just read a recent paper on the distribution of grammatical forms, namely Nichols, "Why Are Stative-Active Languages Rare in Eurasia: A typological perspective on split-object marking" in The Typology of Semantic Alignment (2008). Nichols's approach called "population linguistics" is capable of identifying zones of language spread and language accretion. In a number of publications, this one being the latest, she noted the peculiar absence of ancestral grammatical forms (active-static, head-marking languages) in Northern Eurasia (!). They're widely spread in America, Oceania and in such pockets as Basques and the Caucasus, somewhat common in southern Eurasia (Sino-Tibetan) and Africa but are utterly missing in Northern Eurasia, including Indo-European, Uralic, etc.
She calls Northern Eurasia a vast "spread zone" meaning a territory of constant population movement and language shifts which obliterated those ancestral grammatical forms. Alternatively, America, Oceania, the Caucasus and the Basques are the main refugia preserving older linguistic forms. - Friday, October 16, 2009 4:56:00 pm
- Maju said...
The lack of basal lineages in no way rules a northern Asia route.
It's Chandrasekar's conclusions, not just mine.
As you yourself later say, 'In the long term you won't find L3-underived anywhere, the same you don't find M-underived, etc. All lineages evolve one way or another.
Apples and oranges. Basal lineages are indeed found in tropical Asia and mainland Oceania but in Northern Asia nor in Europe or America, they are all clearly derived of this southern Asian huge top level diversity. The case is very clear.
So why would you expect basal lineages to survive? -
Because we find them in the south and SE but not in the north or NW. Not underived as you claim (absurd!) but highly derived in fact in many many subbranches (depending on the particular lineage). Instead every single northern Eurasian lineage is derived, often several nodes downstream, from a southern one.
And It's possible the authors are a little committed to the idea that India has been important in our origin story?-
And is it possible that you are prejudiced against such idea? You are obsessed up to the point of resulting suspicious with India being driven out of the equation. I am beginning to suspect a racist motivation, conscious or not.
we do actually find some very early N lineages through Central Asia: A, X and, in the Far East, Y/N9.
X, as you know, has a clear West Asian origin. A is highly derived and the obvious product of a most accidental founder effect, N9 looks SE Asian in origin and spread northwards along the coast, as expected. There are 12 basal N lineages and you have what? Only one.
Of course, N does look more SE Asian than South Asian, where N basal lineages are somewhat rare (N2, R, N1'5) but that's not what you are interested in knowing, right? You want to argue your hypothesis until Hell freezes.
Well, Hell has already frozen (cf. Dante). Stop it, please. - Friday, October 16, 2009 5:05:00 pm
- Maju said...
...and Africa...
However, Bickel and Nichols make elsewhere a much less conclusive case for such patterns to matter in fact (and Basque is described as not having that pattern in the very first paragraph, but being ergative instead). Maybe the case that is Bickel and not Nichols the leading researcher here puts the emphasis elsewhere. Slippery science this one of linguistics, indeed. - Friday, October 16, 2009 5:26:00 pm
- Gioiello said...
Maju says: "I think we're all the time talking CRS as in HVS-I, not the full sequence. HVS-2 does not seem to be relevant as is almost never tested".
We can discuss if HVRII isn't "relevant". Anyway we have two mutations between European R0a and the source of H after tens of thousands years. Find you a R closer to H elsewhere. - Friday, October 16, 2009 6:41:00 pm
- terryt said...
"N does look more SE Asian than South Asian, where N basal lineages are somewhat rare (N2, R, N1'5)".
And are most easily explained as being immigrants to South Asia.
"every single northern Eurasian lineage is derived, often several nodes downstream, from a southern one".
What? Two contradictory statements. Unless you're prepared to concede that South Asia is not the source of these southern lineages. If india is not the source how did they get to SE Asia?
"A is highly derived and the obvious product of a most accidental founder effect".
Where from?
"X, as you know, has a clear West Asian origin".
And fairly well north in West Asia. I've even seen Beringia quoted as its origin.
"N9 looks SE Asian in origin and spread northwards along the coast, as expected".
Your theory. - Saturday, October 17, 2009 10:17:00 am
- Maju said...
If india is not the source how did they get to SE Asia? -
Flying in a UFO. *sarcasm*
IMO underived N migrated there with the earliest M ones. N must be younger than M, whatever MCH fans think. You already know I think that - why do you even ask?
Where from? -
As N (the only branched upstream node) should be from SE Asia, from there.
And fairly well north in West Asia.
X1 is more North African than Asian and certainly is not found out of West Eurasia.
I've even seen Beringia quoted as its origin.
Of all X?! Pick better your readings.
Not even X2 has enough basal diversity out of West Eurasia for that even to be a half-reasonably. Obviously the rare North American Native X2 variants are a derivation, the only one in mtDNA, of a West Eurasian lineage. However, it is true that the intermediate steps, excepting some Altaian X2 (of the Western type) are lost by now.
Your theory.
Naturally. - Saturday, October 17, 2009 1:37:00 pm
- German Dziebel said...
I'd be curious to know how do you, Luis, root your trees? Do you use a primate sequence, a Neanderthal sequence, a Mungo man sequence, or a "nuclear insert" in mtDNA?
Reidla et al. (2003, 1181) published an interesting caveat regarding the phylogeny of X haplogroup: "Moreover, haplogroup X is subdivided into two major subhaplogroups, designated "X1" and "X2." Subhaplogroup X1, represented by a single Druze mtDNA in figure 1, differs from the root of haplogroup X by eight coding and three control region transitions and lacks the two transitions (195 and 1719) that characterize X2. These two nucleotides are rather mutable (Finnila ̈ et al. 2001; Herrnstadt et al. 2002); thus, it cannot be completely ruled out that X1 is indeed a subset of X2 that reverted at both nucleotide positions."
I personally haven't seen an article in which X was given a Beringian origin but A, X and (Australian) S are closely related lineages, hence I doubt X emerged in West Asia, as A and S aren't found there. East Asia, Beringia or America make more sense as its places of origin. - Sunday, October 18, 2009 4:44:00 am
- Maju said...
So you think that X2 might not exist... Interesting.
I don't think it changes much, specially when X1 is defined by a single control region marker (which looks much more feeble to me in principle).
A, X and (Australian) S are closely related lineages.
Not anymore than anything else under N: they are just three of 12 known lineages that apparently hang from that node, including macro-haplogroup R.
But anyhow, FYI, I recall that recent study by Alexe et al., who researched all the mtDNA tree all over (using a different method than the usual one of SNPs: K-means analysis) and, while they recycled completely the Eurasian (and American, etc.) part of the tree, even scrapping macro-haplogroup R and other "heresies", the basic structure of the global tree stood their efforts: L1, L2 and L3 (including what we still call M and N) persisted even against such titanic and unconventional efforts, only the structure of M and N (always under L3) was altered.
Anyhow, I think that the paper had little merit - though it's always good to read something that challenges it all, even if it fails. You may enjoy reading it but it's not going to give you the reason on your out of America fantasy. - Sunday, October 18, 2009 6:10:00 am
- German Dziebel said...
"So you think that X2 might not exist... Interesting."
Actually it's X1.
Alexe uses primate sequences to root a tree - this has been criticized. What about the nuclear insert? Without properly rooting a tree, it's hard to say which mutations are basal and which ones are derived. However, phylogeographic observations can be suggestive of the directionality of mutations. If L1, L2, L3 are African-specific, while M and N are found everywhere, including North Africa, I'd interpret M and N mutations as ancestral, while L1-L3 mutations as derived. The diversity within these lineages doesn't really matter as larger population size and gene flow in Africa will make its lineages more diverse than the lineages of more isolated populations with smaller effective population sizes outside of Africa.
By making L1-L3 mutations look ancestral phylogenetically (which is evolution only on a computer screen), the out of Africa theory arrives at a phylogeographic stalemate, with no L lineages found outside of Africa and with no early offshoots of M and N detected in Africa.
Also, Alexe's Fig 3 shows the trifurcation of M, N and L3 and not the subordination of M and N to L3. This looks somewhat like the D-E situation in Y-DNA.
"out of America fantasy."
Out of Africa is a lie, and the bigger the lie the more people believe in it. - Sunday, October 18, 2009 7:00:00 am
- Maju said...
"So you think that X2 might not exist... Interesting."
Actually it's X1.
From your quoted text: [X1] "lacks the two transitions (195 and 1719) that characterize X2. These two nucleotides are rather mutable"...
And if you check PhyloTree, it is clear that the transition at 1719 is the only coding region mutation defining
X2, not X1.
Anyhow, what would seem is that there are some reasons to consider the X1/X2 as at least somewhat doubtful. However X stands and so do the lineages under the X1/X2 nodes, which are essentially West Eurasian (with one or two American exceptions).
Alexe uses primate sequences to root a tree - this has been criticized.
By whom? AFAIK what has been criticized is the use of the supposed but hardly clear Homo-Pan split age for MCH calculations. It is clear that using Pan sequences to root the human phylogeny makes all sense, why wouldn't it?
Without properly rooting a tree, it's hard to say which mutations are basal and which ones are derived.
Seriously, I do not understand what you mean. It's as simple as all human sequences having the defining mutations of L1-6, except those that belong to L0. That makes the root to be between L0 and L1-6. The same method applies to all branches at any level.
I'd interpret M and N mutations as ancestral, while L1-L3 mutations as derived.
You can't because these are sets and subsets. L3 includes M but M does not include L3. M has all the defining mutations of L3 (plus others) but L3 has none of the defining mutations of M.
That's how you root a tree: the inclusive set is always an upstream clade, the distinct subset is always a downstream clade.
Do your homework, seriously. - Sunday, October 18, 2009 3:31:00 pm
- German Dziebel said...
You describe a hypothesis but you don't prove it. Phylogeography seems to disprove your phylogeny, as you don't find L lineages next to M or N in the same geographic area or in the same population. The only place where they're found in the same area is North and Northeast Africa and here M and N are derived from non-African M and N and not from L.
The same concerns America: if America was peopled, you would expect to find R, M and N lineages next to A, X, B, C and D at decreasing frequencies from North to South or you would find only R, M and N lineages because A, X, B, C, and D are either rare or non-existent in geographic areas of Asia leading to America. In actuality, you have the opposite: rare and supposedly recent lineages with a narrow geographic range were brought into America, while more common, older and hence more widely distributed lineages are not found in America.
You and others postulate lineage loss between Africa and, say, India and between Siberia and America but this doesn't explain the special selectivity of these losses twice in human history.
This is exactly how a false theory looks: new facts are retrofitted into a pre-existing idea, namely that America was peopled late in human history, while Africa, with its short and dark tropical people, is the homeland of modern humans.
My theory has no discontinuities: mutations frequently found in Amerindians are original and have the largest geographic distribution. African mutations are derived and have the most restricted geographic distribution. This corresponds to the linguistic map very closely: 140 families in America vs. 20 in Africa.
Instead of arguing with me and showcasing your fundamentalist proclivities, try to devise an mtDNA tree that will illustrate the current distribution of linguistic families. Work with different
tree topologies, root trees using Neanderthal, Mungo man or nuclear insert sequences (nuclear insert is from Zischler et al. 1995), simulate bottlenecks on route to Africa, add gene flow, etc. Use X chromosome, with basal B006 outside of Africa, as a model for a mtDNA phylogeny.
Take 9 bp deletion as an example. This mutation is found in Asia-America, in India and in Africa (high frequencies in Pygmies). What assumptions about mutation rate and bottlenecks do we have to make in order to postulate the common origin of this mutation plus a few SNPs also shared between the sequencies carrying this indel in Asia-America and in Africa? - Sunday, October 18, 2009 5:32:00 pm
- Maju said...
You describe a hypothesis but you don't prove it.
Man: I'm not writing any scientific paper, I'm not the one who has laid out all those theories. I'm just falling into my altruistic outspoken side and trying to help you understand how genetics works. If you don't like it, then it's up to you.
... you don't find L lineages next to M or N...
Whatever. I have told you already a zillion times that M and N are subclades of L3.
You just want to prove that Earth is flat. Fine: it must be an entertaining but unrewarding endeavour. - Sunday, October 18, 2009 7:10:00 pm
- German Dziebel said...
I understand how genetics works, no need to worry. But if you want to help science, rather than simply consume some of its products, you can consider other alternatives. An alternative is already a contribution to science: regurgitation is not.
M and N are not subclades of L3 unless you prove your phylogeny. So far phylogeography disproves it, as well as linguistics, kinship and other cultural elements. Archaeology is neutral, as it can accommodate pretty much any scenario. There're millions of ways in which trees can be generated. There're different tree topologies fitting the same data. Work from X chromosome to Y chromosome to mtDNA to look for alternatives. I suggested to you a few venues to explore. - Sunday, October 18, 2009 7:20:00 pm
- Maju said...
I have my own priorities, which obviously are not the same as yours.
M and N are not subclades of L3 unless you prove your phylogeny.
M and N are subclades of L3 because its phylogeny has been proven: all tested M and N samples have the L3 defining mutations. There is nothing to discuss unless you have evidence of the opposite. - Sunday, October 18, 2009 7:51:00 pm
- German Dziebel said...
Maca-Meyer rooted the tree in a chimp sequence. This is too far in the past and too hypothetical. Plus L3, M and N, even in Maca-Meyer's interpretation, are parallel lineages. Try to root the tree in the Mungo man sequence. It was more divergent than any L lineages. Note also this: "African L3 haplotypes are characterized by the absence of L1/L2-specific polymorphisms at nucleotide positions 769, 1018, 3594, 4104, 7256, 7521, and 13650″ (Herrnstadt et al. 2002. Reduced-Median-Network Analysis of Complete Mitochondrial DNA Coding-Region Sequences for the Major African, Asian, and European Haplogroups. Am J Hum Gen 2002 70 (5).) Finally, Maca-Meyer's tree contains a bunch of homoplasies: 16278 occurs 5 times in different parts of the tree.
Again, this is a hypothesis which, in my opinion, doesn't withstand a test. - Monday, October 19, 2009 1:44:00 am
- Maju said...
What's wrong with rooting using a chimpanzee sequence? Would you prefer it'd be an American animal like a sloth? It would not make any difference, as long as it is an outgroup.
In 2001, L3 was still a tag not applied to N and M, as the phylogeny was still being worked out. However you can clearly see in fig. 1 how L3d and L3b are much more closely related to M and N (and viceversa) than to any other lineage. This is precisely the point of Maca-Meyer.
I'm not sure exactly when the tag L3 began to be used for all the macro-lineage but it's clear that this paper estabilishes the basics of such nomenclature, even if it still doesn't use it.
You're not going to fool me with semantics/nomenclature but I wonder if you can fool yourself. Whatever the name you give to that clade parallel to L2 in fig. 1, it is clear that it is monophyletic. We normally call that L3 and is virtually "official" nomenclature now.
Finally, Maca-Meyer's tree contains a bunch of homoplasies: 16278 occurs 5 times in different parts of the tree.
It can be a recurrent mutation or most likely different mutations at the same locus. This is sadly not too unusual in mtDNA, specially at control area regions (as is the case of all 16... loci) but not enough to blurr the phylogeny. Remember that the mitochondiral DNA is much shorter than any chromosome's and that the control region is just a small fraction of it (however mutating frequently enough as to give some useful keys).
Even if you remove the HVS mutations, the phylogeny stands. I don't think that Hermann's quote changes anything either (after all it's quite obsolete data, from the times L0 and the minor Ls were not even described). Two of those locus define L3 right now (per PhyloTree again). Basically he was noticing, it seems to me mutations describing L3. Back then only L3, L2 and L1 were known I believe (if we don't count M and N), so finding those specificities for L3 surely helped reinforce the distinct personality of this macro-haplogroup.
The current, quite solid, tree was not built in a single paper: it took the accumulative work of many researchers along maybe 15 years or more. - Monday, October 19, 2009 3:39:00 am
- German Dziebel said...
What's wrong with rooting using a chimpanzee sequence? Would you prefer it'd be an American animal like a sloth?
Neanderthal, Mungo man, the nuclear insert are better rooting tools. Without them you can't decide which state was ancestral. Some trees are left unrooted, other trees are rooted.
The rest of your prose is full of apologies. mtDNA studies always had "noise" in them despite the 20 years of research. I would know - I was there. - Monday, October 19, 2009 4:00:00 am
- Maju said...
Neanderthal, Mungo man, the nuclear insert are better rooting tools.
Mungo Man is no valid root: he clearly belonged to H. sapiens. He's no outgroup.
Neanderthal could be valid but the sequence was not known until 2003 and, anyhow, being aDNA, known only in a few specimens, guess it still arises some caution.
No idea what's "the nuclear insert". - Monday, October 19, 2009 4:24:00 am
- German Dziebel said...
The nuclear insert (just like X chromosome's basal B006, it's frequent in Amerindians, rare in Africans, with all others in-between) was used to root the Mungo man sequence.
Zischler, H., Geisert, H., von Haeseler, A., Paabo, S., 1995. A nuclear 'fossil' of the mitochondrial D-loop and the origin of modern humans. Nature 378, 489–492.
The Mungo man sequence was the most divergent, hence, by definition, it fell outside of the modern human mtDNA variation. Anything of this sort can be used as a rooting outgroup. - Monday, October 19, 2009 6:00:00 am
- terryt said...
"The Mungo man sequence was the most divergent, hence, by definition, it fell outside of the modern human mtDNA variation".
And that's the really interesting part, not descnded from L. The fact it appeared in Australia and is clearly 'modern' human sugests that the surviving haplogroups spread through a pre-existing population. And the modern haplogroups may not have their ultimate origin in Africa. They may derive from haplogroups immigrant to Africa. Unlike German I'm quite prepared to accept the current interpretation of haplogroup evolution. - Monday, October 19, 2009 8:07:00 am
- Maju said...
*shakes head*
Mungo Man's remains were ony tested once in what seems to be a very controversial case with not enough guarantees. The remains were given to native tribals (a practice that I strongly oppose in what regards to remains older than say, 1000 years) and will never again be available for comparative re-testing most probably.
Sadly, I don't think that Mungo Man can be used for anything other than speculation at this point.
I see now what the nuclear insert is. IMO, unless it can be shown that somehow the fragment has resisted recombination havoc and is consistent for all humankind, it seems to me that it will also cause many problems and confusion. The Chimpanzee or maybe better the averaged Pan sequence seems the safest so far, though admittedly soon we will be able to contrast the modern human genome with a good quality array of several Neanderthal sequence, which should anyhow produce the same results.
Probably it has already been done, even if only informally, but I'm not aware of it. But otherwise, there would be for sure someone crying foul already. - Monday, October 19, 2009 3:21:00 pm
- German Dziebel said...
There's a theory by which the earliest Australians represent the first migration of anatomically modern humans out of Africa/ Levant (Mungo man's skull seems to cluster with Skhul/Qafzeh in some studies), while the rest of the world was peopled by the second migration (out of Africa, if you're a pygmy-hugger, or out of America if you're a serious scholar) of anatomically and behaviorally modern humans. The first migrants to Australia didn't survive, and the current Australian populations are the result of 2 separate migrations within the past 20,000 years. Hence, linguistically Australia is not as diverse as Papua New Guinea or America.
The nuclear insert is consistent for all mankind: it has all the synapomorphies of modern humans and none of those of Neandertals. - Monday, October 19, 2009 8:09:00 pm
- German Dziebel said...
Luis: "I don't think that Hermann's [read Herrnstadt's - GD] quote changes anything either (after all it's quite obsolete data, from the times L0 and the minor Ls were not even described)."
Luis, Herrnstadt is 2002, while Maca Meyer that you pointed me to is 2001. Which one is more obsolete?
Luis: "Mungo Man's remains were ony tested once in what seems to be a very controversial case with not enough guarantees."
As Kosmo put it the other day in a comment on a different article here, Mungo man results "have been essentially thrown out for no other reason than their wild divergence from expectation."
Well put.
Luis: "Sadly, I don't think that Mungo Man can be used for anything other than speculation at this point."
Everything that you write, including this last sentence, is a huge speculation. I hope you're aware of this.
Terry: "Unlike German I'm quite prepared to accept the current interpretation of haplogroup evolution."
Terry, X chromosome's basal lineage (B006) is most frequent in Amerindians, Y-DNA YAP+ lineages probably migrated into African from Asia and they account for the majority of African Y-DNA diversity.
The nuclear insert is found at high frequencies in America and at low frequencies in Africa, which according to Adcock et al. (the authors of the Mungo man study) implies that humans came to Africa, rather than originated there. African L lineages aren't found outside of Africa in either extant populations or in ancient remains. Non-African M and N lineages aren't indigenous to Africa. Ward et al 1991; Bandelt 2007 estimated that one single Amerindian tribe harbors mtDNA diversity worth of 80,000 years (sic!) of evolution.
All this seems to indicate very consistently that the conventional version of haplotype evolution is wrong. The first mtDNA study, by Johnson et al. 1983 as well as the subsequent study by Excoffier and Langaney 1987 identified out of Asia/out of America as a possible alternative to out of Africa. - Tuesday, October 20, 2009 1:40:00 am
- Maju said...
I would have changed the word "obsolete" if I would have been able to edit. It's not really adequate. Anyhow, I only pointed you to MM because of vane hopes that you could maybe understand that, in spite of your extremely heterodox beliefs, M and N belong to L3, nothing else.
I wonder if this concept has already penetrated your mind or not.
I bet that when we cross paths again in the net you will again insist that M and N are not L3 and all that discourse that just can't hold.
As for Mungo Man. The tests were done apparently with little guarantees, for what I've read, and the fact that the remains have been "returned" to the Aboriginals, making any counter-test nearly impossible, makes us unable to discern the truthfulness or error in this oddball case.
Individual doubtful cases without a context have always been considered with some disregard and extreme caution. Otherwise we'd be talking vividly of the late Acheulean of NW Iberia, contemporary with Magdalenian and other oddball doubtful cases that are normally, when lacking further evidence, archived and disregarded until further evidence appears.
This practice is quite standard in prehistory and extends to many cases.
Anyhow, Mungo Man's apparent mtDNA was extreme but still within the range of modern humans. If you check figure 1 in Caramelli 2003, you can perfectly see that he is within modern human range, with modern day individuals being in some cases further away in the PCA.
Maybe more strange is to me that Cro-Magnon mtDNA is right in the middle of today's European range. Of course they are not that old. - Tuesday, October 20, 2009 6:10:00 am
- Maju said...
And btw, I sincerely think that the comments sections in various blogs are not the place to post your caveats re. the mtDNA phylogeny. You should research the matter and publish something first, at least a series of blog pots, so the rest of the universe can follow your hypothesis consistently.
- Tuesday, October 20, 2009 6:12:00 am
- terryt said...
"the current Australian populations are the result of 2 separate migrations within the past 20,000 years. Hence, linguistically Australia is not as diverse as Papua New Guinea or America".
I'd agree with two (or more) migrations to Oz. However I'd put the first at more like 50k although I agree that many lines involved in that arrival have died out. A second migration seems to have arrived between 20k and 15k. The accepted reason for the lack of linguistic diversity through much of Oz (ignoring the far northwest) is that extreme drought exterminated the population through Central Oz but this region was later recolonised from within the continent.
"X chromosome's basal lineage (B006) is most frequent in Amerindians, Y-DNA YAP+ lineages probably migrated into African from Asia and they account for the majority of African Y-DNA diversity".
I agree completely that haplogroups don't tell the full story by any means. Individual genes move through individual species.
"Mungo Man's remains were ony tested once in what seems to be a very controversial case with not enough guarantees".
That is correct and so I agree we can't rely too much on the particular research. - Tuesday, October 20, 2009 6:31:00 am
- German Dziebel said...
Luis: "Anyhow, Mungo Man's apparent mtDNA was extreme but still within the range of modern humans."
Check Adcock et al. 2001, Fig. 2A and you'll see that the range of variation in ancient Australian sequences overlapped with modern human sequences with LM3 falling outside of it. Nuclear insert overlaps with LM3 but is entirely outside of modern human variation.
"I sincerely think that the comments sections in various blogs are not the place to post your caveats re. the mtDNA phylogeny."
You started it by providing people with your grand theory of human haplotype dispersals. I just asked you about your rooting sequence. You didn't know what it was. I explained.
All my personal comments are relevant to the Chandrasekar paper under discussion, which again reiterated the lack of ancient M and N lineages in Africa and the lack of L3 lineages in India. This has implications for mtDNA phylogeny. I wrote a whole book and a post on anthropology.net outlining my views, so I think I did my job at this time. Now your job is to buy the book, educate yourself about kinship systems and linguistics and if you have any concerns you can write a post anywhere with a critique of the data presented. But your dogmatic belief in the infallibility of the out of Africa theory (mind you, you are not even a scientist, so what are your stakes?) deprives us of a minimal common denominator to interact without bickering.
I am planning to revise the mtDNA phylogeny to make it more consistent with phylogeography, demography, kinship systems and linguistics and to remove the "noise." However at this point I'm still waiting for a good genetics publication to provide me with a springboard.
"Anyhow, I only pointed you to MM because of vane hopes that you could maybe understand that, in spite of your extremely heterodox beliefs, M and N belong to L3, nothing else."
L3 is closer to M and N than either of them to L0, L1, L2, L5, etc. But being closer to doesn't mean they derive from L3. This is up in the air for me because of homoplasies, lack of the necessary mutations on the actually attested sequences, phylogeography, etc.
"As for Mungo Man. The tests were done apparently with little guarantees, for what I've read, and the fact that the remains have been "returned" to the Aboriginals, making any counter-test nearly impossible, makes us unable to discern the truthfulness or error in this oddball case."
This case looks "oddball" only because out of Africa was prematurely made into a measuring stick. There was nothing wrong with the Mungo man research. Yes, it's an unusual case of DNA extraction from a specimen of such antiquity and in such low latitudes (although the DNA from the Windover brain in Florida was also a case of remarkable preservation) but this doesn't make it invalid. Especially since it overlapped with an independently attested nuclear insert. It'd be good to replicate the results, sure, but if this is impossible I'd focus on trying to extract DNA from one of those African skulls to see how far in time L lineages go. - Tuesday, October 20, 2009 4:28:00 pm
- Maju said...
Now your job is to buy the book...
I'm not going to buy that book. I already know more than what I need to know about your kinship-based hypothesis and I know also that it is bad science. Maybe if it was fantasy genre...
L3 is closer to M and N than either of them to L0, L1, L2, L5, etc. But being closer to doesn't mean they derive from L3.
The branch that you seem to be calling "L3" in Maca-Meyer 2001 is actually L3b (or if you wish L3b'd'e'j), not all L3(xM,N). Only L3b and L3e (both within L3b in the most recent PhyloTree nomenclature) are analyzed in that study among "African" L3(xM,N)
Nomenclature changes and the least you could do is to check PhyloTree for the most actualized references. Do your homework, please (I'm not getting paid for these lessons). - Tuesday, October 20, 2009 8:29:00 pm
- German Dziebel said...
The fact that people call a clade to which M and N supposedly belongs "L3" is simply an out of African driven convention. M, N and L3x are sister clades with a tenuous connection to L1, L2 or L0. Think of D and E in Y-DNA as an example. Nobody calls Y-DNA D lineages E lineages. In the same way as D is at least as diverse as E or maybe more, L3 is as diverse or maybe less than M or N.
How can a person who isn't a scientist judge what science is and what is not? I have two doctorates and 20 years of experience across several science fields, including pop genetics. You are an amateur with huge gaps in knowledge, logic and ethics. At times your prose falls somewhere in the gray area between spam and cyberbullying. - Tuesday, October 20, 2009 9:00:00 pm
- Maju said...
Que no! M and N sister clade is not L3 as such but the various L3 sublineages as mentioned above, you are stubborn in this regard because you don't want to face reality. Do your homework and make some self-criticism.
For what I can read at Wikipedia-Scientific method, some guy named Charles Sanders Pierce, stated that the following are from worst to best the ways of finding the truth among humans:
1. The method of tenacity — persisting in that which one is inclined to think.
2. The method of authority — conformity to a source of ready-made beliefs.
3. The method of congruity or the a priori or the dilettante or "what is agreeable to reason" — leading to argumentation that gets finally nowhere.
4. The scientific method — whereby inquiry actually tests itself and criticizes, corrects, and improves itself.
You are stuck in method 1, you don't even care about method 2 and you are very far away from method 4 (science). You just have to be right no matter what.
It's boring, annoying and dismaying to discuss with you. - Tuesday, October 20, 2009 9:18:00 pm
- German Dziebel said...
At least, I made you read Peirce (note the correct spelling, smartie pants!) if not in the original. I have a whole chapter on Peirce in my book because he was one of the first students of kinship-based or relational logic in the 19th century.
"M and N sister clade is not L3 as such but the various L3 sublineages as mentioned above."
Very good. Now D lineages form an outgroup to all Asian and North-East African M lineages, while A, X, S and some other Eastern N lineages form an outgroup to the European-North African (Western) N lineages (including U). Some of the M and N lineages are found in Africa as well as outside of Africa, while all African "L3" lineages are restricted to Africa. African L3 sublineages are derived from certain lineages grouped under Western N submacrohaplogroup, U lineages included. I'm still working on the rest of African L lineages (L0, etc.) but most likely they're derived from X, B and some other "Eastern" lineages.
This is the tree topology that fits phylogeography, linguistics, kinship, etc. better. And it'll have fewer homoplasies than the current one.
Don't argue with it. It's a working hypothesis. I'll publish a revision at some point with all details in it. - Tuesday, October 20, 2009 9:42:00 pm


text:
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And take up other addictions instead. Over the weekend, Yahoo announced it will buy employees the smartphone of their choice so long as it is not a BlackBerry. The company will however, pick up the tab with a data plan for the brand new iPhone 5 and the yet-to-be-released Windows Phone 8.
MONTAGNE: The move, announced in a memo by Yahoo's new CEO is great for tech-loving employees but it's bad news for BlackBerry maker Research in Motion. The tech media website CNet observes that Yahoo's decision to dump the BlackBerry is essentially a bet that the platform is dead.
And that's the business news from MORNING EDITION. I'm Renee Montagne.
INSKEEP: And I'm Steve Inskeep. Transcript provided by NPR, Copyright NPR.

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Dogs occasionally develop a red eye. The redness may be in the white of the eye, in the clear part of the eye called the cornea, or within the eyeball. There are many causes of a red eye, but all ocular diseases should always be considered an urgent matter, and be checked immediately by a veterinarian.
A dog owner will typically be asked to describe the pet's medical history, symptoms, and if there were any pertinent events prior to the development of the red eye, such as injury or illness. The veterinarian will perform a complete physical exam on any dog that has a red eye to look for any other abnormalities that may give a hint as to their cause. The veterinarian will then concentrate on the eyes. There are three basic ophthalmic tests: a Schirmer test, tonometry, and fluorescein staining.
A Schirmer test measures tear production. Special paper strips are placed in each eye and it is noted how many millimeters of wetness the tears can form in 60 seconds. Too little tear production is called keratoconjuntivitis sicca (KCS) or "dry eye" is the common name. Tonometry is used to measure the pressure inside the dog's eyes; increased pressure indicates glaucoma. Fluorescein staining checks for any scratch or interruption of the outside covering of the cornea. A positive stain indicates a corneal ulcer.
There are other tests that may be performed. The veterinarian may swab any pus draining from the dog's eyes and send it to the laboratory for testing. Lab work, including a chemistry panel, a complete blood count, an electrolyte panel and a urinalysis may be performed to check for underlying diseases. An ultrasound of an eye may be done to check for a detached retina or masses within the eye or between the eye and the bony eye socket.
A veterinarian diagnoses the cause of a dog's red eye by assessing symptoms, medical history, physical examination, and laboratory testing. The most common causes of a red eye are a corneal ulcer, glaucoma, and conjunctivitis.
Corneal ulcers usually result from trauma such as running into a bush, fighting with other pets, or rubbing their eye. Ulcers are treated with topical antibiotics, and checked with fluorescein stain every few days until healed.
Glaucoma can occur in any dog, is sometimes related to other conditions such as cataracts, but is genetic in some breeds. Cocker Spaniels, terriers, Poodles, Basset Hounds, and Beagles are especially prone to this disease. Glaucoma can be very serious, it often leads to blindness. There are topical medications used to reduce the increased pressure.
Conjunctivitis can have many causes. Dry eye causes a conjunctivitis; this is treated with a topical medication that increases the amount of tears produced. Conjunctivitis can also be caused by bacterial infections, viral infections, or other irritants.
There are many other causes of a red eye: uveitis, orbital disease, bleeding disorders, trauma, luxated lens, etc. Your veterinarian can sort through all the possibilities and determine the appropriate treatment.
American Kennel Club, Canine Health Foundation. "Canine Eye Health." November 2013.
Barfield Laminack, Elizabeth, DVM; Myrna, Kathern, DVM, MS; Moore, Phillip Anthony, DVM, Diplomate ACVO. "Clinical Approach to Canine Red Eye." Today's Veterinary Practice, May/June 2013.


text:
In Flowering-Plum village at the foot of Luofu Mountain,
The flowering plums' bones are of jade and snow, and their souls of ice.
In their multitude the blossoms seem moonlight hanging from the trees,
In their brightness they are alone with Orion on the horizon at dusk.
By Su Shi蘇軾 (1037–1101)Su Shi "Using the Same Rhyme Words Again" translated by Hans Frankel in Maggie Bickford et. al., Bones of Jade, Soul of Ice: The Flowering Plum in Chinese Art, Yale University Art Gallery, 1985, p. 169.
Ubiquitous in Chinese painting, poetry, ornament, and in nature, the flowering plum (mei hua梅花), is uncontested as China's favorite blossom. Over the centuries it has acquired a wealth of meaningful associations, not only foretelling the welcome coming of spring but also identified with understated elegance, the fragility and transience of female beauty, and the solitary enjoyment of nature by the noble recluse, detached from worldly pursuits. These are but two of many concepts which over time became indelibly associated with the blossoming plum.Maggie Bickford has discussed in depth the flowering plum and its associations in Ibid. and in Ink Plum, the Making of a Chinese Scholar-Painting Genre. Cambridge University Press, 1996. This essay is based in large part on her publications. I take all responsibility for errors.
While the blossoms are short-lived, the aged branches and hoary trunk suggest longevity, an auspicious association which also accrued to the Three Friends of the Cold Season. Joined with evergreen pine and bamboo, the flowering plum constitutes the Three Friends of the Cold Season (suihan sanyou 歲寒三友), which, in addition to longevity, symbolize the integrity and high-mindedness of the Confucian scholar and his ability to endure adversity in difficult times.
Not only Chinese but also Japanese and Korean artists have expressed enthusiasm for the flowering plum, its auspiciousness and distinctive beauty.
Before further exploring the associations of the flowering plum and the correspondence between style and patronage, it is worth reviewing briefly botanists' classification of the flowering plum. While in Chinese the tree is referred to as mei and the blossoms as mei hua, in European languages it has no definitive name. According to botanist Li Hui-lin, "Together with peach, almond, cherry and other flowering and fruiting trees and shrubs, these plants (mei) belong to the genus Prunus. In 1836, the mei hua was designated Prunus mume by the botanists Siebold and Zuccarini, based on their observation of plants growing in Japan. Mume may be derived from ume, the Japanese pronunciation of the Chinese mei, or it may actually represent a combination of mei and ume."Li Huilin, "A Botanical Note" in Bickford et al, Bones of Jade, Soul of Ice, p.245.
Li's research in ancient texts indicates that mei is indigenous to southern Shaanxi province in North China and was exported to Japan in early times. Today, the flowering plum grows wild in central and southwestern China and is cultivated throughout China, especially in the Yangzi and Pearl River basins, as well as in Japan, the United States and beyond.Ibid.
The distinctive characteristics of the flowering plum's appearance in nature and its growth cycle are the source of its many associations. The blossoms have five petals, most often snowy white, which flower and fall well before the leaves appear. Since for the Chinese the number five is auspicious, the fact that each blossom has five petals makes the plum (mei) an auspicious symbol, while its whiteness and blossoming in late winter, when ice and snow still blanket the ground, associate it with integrity, purity, and resilience under adverse circumstances.
Moreover, the plum tree flowers as the cold weather approaches its end, making it the harbinger of spring. In his poem entitled "The Flowering Plum," Xiao Gang蕭綱 (503–551), who reigned as the Liang emperor Jianwen簡文 (549–551), writes,
The flowering plum is the earliest to blossom,
She alone has the gift of recognizing spring.Xiao Gang, "The Flowering Plum" translated by Hans Frankel in Ibid, p.155.
Many flowering plum trees live more than one hundred years, so the aged trees are also associated with longevity. As they age, the trunk and branches become gnarled and angular, while young branches, heavily laden with blossoms, and whip-like shoots issue forth in all directions. This growth pattern has great visual appeal.
When all these characteristics and related associations are taken into account, it is no wonder that the flowering plum (mei) gave rise in literature to an abundance of plum poetry and in the visual arts, it inspired, among other things, colorful, superbly detailed plum painting at the Imperial court, and the distinct genre of ink plum painting favored by the literati. A masterwork of Chinese art in the Seattle Art Museum collection exemplifies the understated beauty of ink plum painting.
The Ink Plum (momei 墨梅)
In the Northern Song dynasty (960–1126) and earlier, the flowering plum appears in poetry and painting, but it was in the twelfth and thirteenth centuries, after the Song court was forced by the Jurchen invasion of North China to move south in 1127 and subsequently made Lin'an (modern Hangzhou) their capital, that an obsession with the flowering plum took hold. In South China the plum grew in abundance. A few late Song plum cultists took appreciation to extremes. The Southern Song recluse Lin Hong林洪 (active ca. 1131-61) wrote instructions for building a "Plum-Blossom Paper Tent梅花紙帳" and the scholar-official Zhang Zi張鎡(1153—1212)detailed the likes and dislikes of the blossoms. Bickford et al., Bones of Jade, Soul of Ice, pp. 30-43.
In his essay on the evolution of plum poetry, Professor Hans Frankel remarks on the increased importance of plum imagery among the literati: "Especially from the twelfth century on, practically every poet felt obliged to devote some poems to the blossoming plum, which was considered superior to other trees and spiritually akin to the man of letters."Hans Frankel, "The Plum Tree in Chinese Poetry," Asiatische Studien 6 (1952), p. 88. In painting, the literati, or man of letters, eschewed formal training in favor of spontaneous self-expression closely allied to the art of calligraphy. Since the educated man wrote with the brush throughout his life, the brush was a natural extension of his arm and an appropriate tool to express deep thoughts and innermost feelings. As Su Shi noted,
Why should the superior man study painting?
The use of the brush comes naturally to him.Su Shi, "Ciyun shui guan shi" 次 韻 水 官 詩, translated by Susan Bush, The Chinese Literati on Painting: Su Shih (1037-1101) to Tung Ch'i-ch'ang. Harvard University Press, 1971, p. 36.
Literati painting (wenren hua文人畫), the antithesis of decorative beauty, did not seek formal likeness. Instead, the scholar-painter passed the natural image through the filter of his mind, a mind characterized by cultivation, spontaneity, and sensitivity to nature. Wielding brush and ink, the literati painted for their own pleasure or to communicate with like-minded friends, embedding themselves in meaningful images, including the monochrome ink plum (momei).Bickford, Ink Plum and Bickford, Bones of Jade, Soul of Ice, pp. 56-150.
The ink plum tradition (momei), founded by the Chan monk Zhongren仲仁 (d. 1123) and further developed during the Southern Song, gained added significance during the Yuan dynasty (1279–1368), when the Mongols ruled China. Exemplifying endurance under adversity, the flowering plum was a favored subject and certain scholar-painters, most notably Wang Mian王冕 (d. 1359), concentrated solely on the ink plum, creating powerful images of dramatic intensity. Beginning in the Southern Song, plum manuals were in circulation, such as Meihua Xishen pu梅花喜神譜 by Song Boren宋伯仁 (active ca. 1240) that trace the life cycle of the flowering plum, matching each with a poem, or Wu Taisu 吳大素's Songzhai meipu 松齋梅譜 (ca. 1351), codify the multitude of ways to paint flowering plum, explicating the "do's and don'ts."Bickford, Ink Plum, p.185-196; Shimada Shujiro, "Shosai baifu teiyo,"1956.
Yang Hui's A Branch of the Cold Season (suihan zhi嵗寒枝)Among the superb ink-plum paintings by less well-known early Ming masters is A Branch of the Cold Season (Sui han zhi ) 歲寒枝 by Yang Hui楊輝, from the early fifteenth century, a jewel in the collection of the Seattle Art Museum. The composition is masterful, the verticality of the curving trunks, branches and shoots on the right balanced by the horizontality and effortless grace of the extended branch extending out to the left. Above the branch, an inscription written by the artist's contemporary, monk Zhen, details the circumstances of the painting's creation, while below a prominent red seal identifies the scroll as having once belonged to the distinguished Tayasu House of the Tokugawa family.As one of the Gosankyo, or three branches of the Tokugawa clan, the Tayasu family started from Tokugawa Munetake (1716–1771), who was the second son of the eighth Shogun Tokugawa Yoshimune, and this lineage continues today. Throughout, the brushwork is playful, full of energy and verve.
While Yang Hui was clearly conversant with modes of plum painting and plum manuals, his use of brush and ink is delightfully natural and fresh, devoid of any trace of artifice. For example, in the Seattle painting, the artist freely varies the pace of his strokes, suggesting a bud with a quick turn of the brush or speedily outlining a petal, deftly varying the pressure on the brush so that the width of the stroke suddenly widens and suddenly narrows, the branch visible beneath. Whereas Wang Mian's brush handling is beautifully controlled and consistent, Yang Hui's is spontaneous, unpredictable and full of vitality.
Yang Hui's A Branch of the Cold Season, like the preponderance of plum paintings, conveys a message of goodwill and auspiciousness. Monk Zhen's inscription reveals that Yang Hui painted this parting gift for his friend Mr. Hong Suichu (Hong Chun洪春, 15th c.), who is about to leave for Fujian, as an expression of "inseparable friendship" and wishes for top honors in the civil service examinations. The inscription reads as follows:
"Mr. Hong Suichu [Hong Chun, 15th c.] is about to take a trip to Qimin (Fujian). His friend, saddened by his departure, did A Branch of the Cold Season (Suihanzhi), as a present for him. This is not only an expression of their inseparable friendship, but also a sincere wish [for the top honor in the examination] that someday Mr. Hong will win the prize of the 'Earliest Spring' and be 'champion of all flowers.' May Mr. Hong treasure it!"
Then, Monk Zhen composes a poem, citing the early Song poet Lin Bu林逋 (967–1028), who was an ancestor of the above-mentioned Lin Hong and "the exemplar of the flowering-plum recluse":
When the plum blossoms begin to bloom by the [West Lake] home of the Immortal Bu [Lin Bu, 967–1028],
Your boat will be sailing from the [Qiantang] River of the former premier Wu Yun [d. 484 b.c.].
Imagine some of these nights you will be thinking of each other in the bright moonlight.
The "dimly stirred fragrance" and the "sparsely interweaving shadows" will follow you wherever you go.
[signed]: At Huan'an [Temple], Shanxi, Monk Zhen
The poem refers to Lin Bu's celebrated couplet:
Its sparse shadows are horizontal and slanted—the water is clear and shallow;
Its hidden fragrance wafts and moves—the moon is hazy and dim.
—Lin BuLin Bu "Flowering Plum in the Garden on the Hill" translated by Hans Frankel in Bickford et al., Bones of Jade, Soul of Ice, p. 165.
Plum blossoms and moonlight, long seen as connecting those who are separated geographically, will link the two friends, Yang Hui and Hong Suichu, wherever they go. The literary and painterly imagery of Yang Hui's A Branch of the Cold Season is evocative, resonating with the past and effectively combining poetry, painting and calligraphy, which are together known as The Three Perfections.Michael Sullivan, The Three Perfections, Chinese Painting, Poetry and Calligraphy. Thames and Hudson, 1974.
New Discoveries Confirm Artist: Yang Hui of early Ming
The Seattle Art Museum hanging scroll, A Branch of the Cold Season, has long been dated to the Yuan period and compared to ink plum paintings of the fourteenth century, especially those by Wang Mian.Bickford, Ink Plum, pp. 199-200; Bickford et al, Bones of Jade, Soul of Ice, pp. 72-72. The Yang Hui seal on the painting led to the assumption that it was painted by the fourteenth-century Yang Hui, a mathematician of the Yuan dynasty who also excelled at painting. While working on the Seattle Art Museum's online catalogue, art historian Wang Yao-ting clarified that the Seattle painting must be fifteenth century, or early Ming dynasty, based on the fact that the recipient Hong Chun, also known as Hong Suichu, is recorded to have passed the fuxue maocai 府學茂才 examination during the Zhengtong period (1436-49).Lang Ying 郎瑛 (1487–ca. 1566), Qi xiu lei gao七修類稿, juan 31. Moreover, while the life of Monk Zhen of Huan'an Temple remains unknown, his poems are found in poetry collections of Ming writers.Based on Wang Yaoting correspondence with Josh Yiu. This evidence supports a fifteenth century, or Ming dynasty date, for the painting and its artist.
Given the fact that the scroll has no artist's signature, an important issue arises. Do the three seals on the Seattle ink plum painting (Zhong he仲和, Liangjian qing qi兩間清氣,We are grateful to Professor Kawai Masatomo and art historian Yukiko Shirahara, Chief Curator of the Nezu Museum, for bringing the hanging scroll in the Kyushu National Museum to our attention.and Yang Hui楊輝) belong to the painter of this marvelous ink plum or simply to a person who saw it or owned it? The remarkable discovery of another painting by the same hand leaves little doubt that Yang Hui (15th c.) is the artist of the Seattle ink plum.
A Second Ink Plum Painting by Yang Hui of Early Ming
In 2010 Professor Kawai Masatomo brought to our attention an ink plum painting in a private Japanese collection (fig. 1), which not only is strikingly similar stylistically to the Seattle ink plum, but also bears an identical seal, Liangjian qingqi 兩間清氣. In the painting of flowering plum in Japan, the positioning of this seal in the upper right leaves little doubt that it was the painter's seal. Its positioning in the Seattle painting is also revealing. On the center left, the Liangjian qingqi seal is located squarely between and in close proximity to the Zhonghe and Yang Hui seals, indicating that three seals belong to the same person: Yang Hui of the fifteenth century.
Unfortunately no factual information about Yang Hui of early Ming has come to light; however, his Liangjian qingqi seal and the poem by Wang Mian, which Yang inscribed on his ink plum painting now in Kyushu, suggests that he was an admirer of the classic Yuan dynasty plum master who died in 1359. The meaning of Yang Hui's seal Liangjian ("between heaven and earth") qing qi (pure air or spirit, referring to blossoming plum), refers to a well-known Wang Mian poem, inscribed on a large album leaf, entitled Ink Plum, in the Palace Museum, Beijing (fig. 2). Wang Mian writes:
On the trees beside my family's Washing-Inkstone Pond,
One by one, the blossoms open, bearing traces of pale ink.
They don't want men to boast of their fine color.
There only flows their pure spirit, suffusing heaven and earth.Wang Mian's poem as translated by Maggie Bickford. Ink Plum, p. 209; pl. 26; see also note 50, p. 259, which states that a similar version of this poem is found in Wang Mian's collected works.
Once again it is the purity of the flowering plum and its subtle, nuanced beauty which is captured in painting and poem.
Stylistically Yang Hui's two paintings (the one preserved in Seattle Art Museum and fig. 1) are not only remarkably alike in composition and execution—especially if you rotate the hanging scroll in the Japanese collection to a horizontal position—they also suggest the abiding influence of Wang Mian (fig. 2) on the ink plum tradition. Clearly the compositional similarities between Wang Mian and Yang Hui's two works reflect the distinctive natural growth patterns of the flowering plum tree, yet Yang Hui clearly belongs to the literati ink plum painting tradition, in which Wang Mian, who lived just one century earlier, was the dominant figure.
While the life of Yang Hui of the Ming dynasty remains a mystery, his paintings, especially Seattle's A Branch of the Cold Season, attest to his fresh, playful approach to wielding the brush and express his sadness over the departure of his friend and hope for his success in the exams which he likens to the triumphant flowering of the plum in the cold.
© 2013 by the Seattle Art Museum
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Yang Hui楊輝
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Ubiquitous in Chinese painting, poetry, ornament, and in nature, the flowering plum (mei hua梅花), is uncontested as China's favorite blossom. Over the centuries it has acquired a wealth of meaningful associations, not only foretelling the welcome coming of spring but also identified with understated elegance, the fragility and transience of female beauty, and the solitary enjoyment of nature by the noble recluse, detached from worldly pursuits.


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"Into You" was released in May 2016 as the second single from Ariana Grande's third studio album, Dangerous Woman. The song was received positively, with critics calling it "catchy" and "a massive club tune".


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Circe (Paperback)
April 2018 Indie Next List
"This remarkable journey into mythology brings the ancient gods directly and viscerally into the present. Circe is a perfect mashup of elegant language, glorious storytelling, and exquisitely modern sensibilities. Miller's telling left me awed and moved by Circe and her story, all while wishing I could invite her over for a glass of wine on the porch. How this amazing author so perfectly melds the human and the divine, creating a story both immediate and epic, is dazzling."
— Beth Albrecht, The Magic Tree Bookstore, Oak Park, IL
This #1 New York Times bestseller is a "bold and subversive retelling of the goddess's story" that brilliantly reimagines the life of Circe, formidable sorceress of The Odyssey (Alexandra Alter, TheNew York Times).
In the house of Helios, god of the sun and mightiest of the Titans, a daughter is born. But Circe is a strange child -- not powerful, like her father, nor viciously alluring like her mother. Turning to the world of mortals for companionship, she discovers that she does possess power -- the power of witchcraft, which can transform rivals into monsters and menace the gods themselves.
Threatened, Zeus banishes her to a deserted island, where she hones her occult craft, tames wild beasts and crosses paths with many of the most famous figures in all of mythology, including the Minotaur, Daedalus and his doomed son Icarus, the murderous Medea, and, of course, wily Odysseus.
But there is danger, too, for a woman who stands alone, and Circe unwittingly draws the wrath of both men and gods, ultimately finding herself pitted against one of the most terrifying and vengeful of the Olympians. To protect what she loves most, Circe must summon all her strength and choose, once and for all, whether she belongs with the gods she is born from, or the mortals she has come to love.
With unforgettably vivid characters, mesmerizing language, and page-turning suspense, Circe is a triumph of storytelling, an intoxicating epic of family rivalry, palace intrigue, love and loss, as well as a celebration of indomitable female strength in a man's world.
#1 New York Times Bestseller -- named one of the Best Books of the Year by NPR, the Washington Post, People, Time, Amazon, Entertainment Weekly, Bustle, Newsweek, the A.V. Club, Christian Science Monitor, Refinery 29, Buzzfeed, Paste, Audible, Kirkus, Publishers Weekly, Thrillist, NYPL, Self, Real Simple, Goodreads, Boston Globe, Electric Literature, BookPage, the Guardian, Book Riot, Seattle Times, and Business Insider.
About the Author
Madeline Miller is the #1 New York Times bestselling author of two novels: The Song of Achilles, which won the Orange Women's Prize for Fiction 2012, and Circe, which was short-listed for the Women's Prize for Fiction 2019. Her books have been translated into over thirty two languages. Miller holds an MA in Classics from Brown University, studied in the Dramaturgy department at Yale School of Drama, where she focused on the adaptation of classical texts to modern forms, and taught Latin, Greek, and Shakespeare to high school students for over a decade.
Praise For…
Winner of the 2019 Indie Choice Award
Shortlisted for the 2019 Women's Prize for Fiction
Named one of the 'Best Books of 2018' by NPR, The Washington Post, Buzzfeed, People, Time, Amazon,Entertainment Weekly, Bustle, Newsweek, the A.V. Club, Christian Science Monitor, Southern Living,and Refinery 29.
"Circe,' [is] a bold and subversive retelling of the goddess's story that manages to be both epic and intimate in its scope, recasting the most infamous female figure from the Odyssey as a hero in her own right."—Alexandra Alter, New York Times
"One of the most amazing qualities of this novel [is]: We know how everything here turns out - we've known it for thousands of years - and yet in Miller's lush reimagining, the story feels harrowing and unexpected. The feminist light she shines on these events never distorts their original shape; it only illuminates details we hadn't noticed before."—Ron Charles, Washington Post
"[Miller] gives voice to Circe as a multifaceted and evolving character...'Circe' is very pleasurable to read, combining lively versions of familiar tales and snippets of other, related standards with a highly psychologized, redemptive and ultimately exculpatory account of the protagonist herself."—Claire Messud, New York Times Book Review
"The story of Circe's entanglement with Odysseus lasts far beyond the narrative of "The Odyssey," making for compelling material to revisit. But ultimately it's as a character that Circe stands apart....Through her elegant, psychologically acute prose, Miller gives us a rich female character who inhabits the spaces in between."
—Colleen Abel, Minneapolis Star Tribune
"Miller's lush, gold-lit novel - told from the perspective of the witch whose name in Greek has echoes of a hawk and a weaver's shuttle - paints another picture: of a fierce goddess who, yes, turns men into pigs, but only because they deserve it."—NPR.org
"so vivid, so layered, you could get lost in it... Whether or not you think you like Greek Mythology, this is just great storytelling. It feels cinematic."—NPR's Here & Now
"Miller's spell builds slowly, but by the last page you'll be in awe. In prose of dreamlike simplicity, she reimagines the myth of Circe."—People
"Miller, with her academic bona fides and born instinct for storytelling, seamlessly grafts modern concepts of selfhood and independence to her mystical reveries of smoke and silver, nectar and bones."—Entertainment Weekly
"This telling, in the sorceress's own words, is not the version we think we know."—New York Times 'T Magazine'
"Miller gives voice to a previously muted perspective in the classics, forging a great romance from the scraps left to us by the ancients....Circe is, instead, a romp, an airy delight, a novel to be gobbled greedily in a single sitting."—Aida Edemariam, Guardian
"In Madeline Miller's "Circe" - the gorgeous and gimlet-eyed follow-up to her Orange Prize-winning first novel, "The Song of Achilles" - the goddess is young and romantic enough at the start to feel a tiny bit let down that she's not shackled to a rock like her uncle, Prometheus, getting her liver pecked out each day."
—Laura Collins-Hughes, Boston Globe
Ambitious in scope, Circe is above all the chronicle of an outsider woman who uses her power and wits to protect herself and the people she loves, ultimately looking within to define herself. Readers will savor the message of standing against a hostile world and forging a new way."—Shelf Awareness
"A retelling of ancient Greek lore gives exhilarating voice to a witch... [Circe is] a sly, petulant, and finally commanding voice that narrates the entirety of Miller's dazzling second novel....Readers will relish following the puzzle of this unpromising daughter of the sun god Helios and his wife, Perse, who had negligible use for their child....Expect Miller's readership to mushroom like one of Circe's spells. Miller makes Homer pertinent to women facing 21st-century monsters."—Kirkus, Starred Review
"An epic spanning thousands of years that's also a keep-you-up-all-night page turner."—Ann Patchett, author of Commonwealth
"With lyric beauty of language and melancholy evocative of Keats' "Ode on a Grecian Urn", CIRCE asks all the big questions of existence while framing them in the life story of the famous goddess who had the magic of transformations. A veritable Who's Who of the gods of Olympus and the heroes of ancient Greece, Circe knows them all and we see them through her perceptive eyes. This is as close as you will ever come to entering the world of mythology as a participant. Stunning, touching, and unique."—Margaret George, author of The Confessions of Young Nero
"Circe bears its own transformative magic, a power enabled by Miller's keen eye for beauty, adventure, and reinvention. Through the charms of a misfit heroine, the world of gods becomes stunningly alive, and the world of our own humanity--its questions, loves, and bonds--is illuminated. This book is an immense gift to anyone who reads to find their own bravery and quest."—Affinity Konar, author of Mischling
"Madeline Miller, master storyteller, conjures Circe glowing and alive - and makes the Gods, nymphs and heroes of ancient Greece walk forth in all their armored splendor. Richly detailed and written with such breathtaking command of story, you will be held enchanted. A breathtaking novel."—Helen Simonson, author of The Summer Before the War and Major Pettigrew's Last Stand
"Circe is the utterly captivating, exquisitely written, story of an ordinary, and extraordinary, woman's life"—Eimear McBride, author of A Girl is a Half-Formed Thing
"Written with power and grace, this enchanting, startling, gripping story casts a spell as strong and magical as any created by the sorceress Circe."—Mary Doria Russell, author of Epitaph
"Madeline Miller's re-imagining of the witch Circe from The Odyssey makes for an intriguing, feminist adventure novel that is perfectly suited for the #TimesUp moment. Circe is also a smart read that has much to say about the long-term consequences of war and a culture that values violence and conquest over compassion and learning...Miller mines intriguing details from the original tale to imagine a rich backstory for Circe that allows readers to re-visit the world of Olympians and Titans in Greek mythology. From the court of the Titans, the reader meets Circe's parents, the god Helios and nymph Perse, and is introduced to a world of supernatural power players that is every bit as back-biting, gossip-filled and vicious as any episode of House of Cards."
—May-Lee Chai, Dallas News
"'Circe' is a sentence-by-sentence miracle";—Michigan Daily


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(Minghui.org) A "Solemn Declaration" is a person's public statement to the world that whatever he or she has done or said under duress or deception that was against Falun Dafa is null and void. These statements have come from Falun Dafa practitioners in China who wish to express regret that, in the face of physical torture and brainwashing, they signed documents renouncing Dafa and guaranteeing not to practice again. Now, they hereby declare their intention to resume practicing Falun Dafa.
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This listing is for the Cardback Restoration Kit:
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- Each card is discretely marked as a restoration item on the back
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Resto Kit - Boushh - POTF
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Who hasn't had neck pain at one time or another? What's more, many of us have experienced on-going neck troubles at some point during our lives. Looking at human anatomy, it's no wonder pain strikes us so often in this vulnerable area. While the neck structure gives us an amazing range of movement with which to see our environment, it also leaves us prone to injury of the muscles, ligaments, tendons and joints. But by making regular visits to the chiropractor, paying attention to posture and doing regular stretching and strengthening exercises, our necks can be pain-free.
Frequent chiropractic adjustments help prevent neck pain from occurring in the first place, but some everyday activities such as poor posture during watching TV, using a computer, reading a book or talking on the phone can easily trigger neck pain. Here are a few tips for avoiding neck pain throughout your day:
- Pay attention to your posture. Check with your chiropractor for guidelines to help improve your everyday posture.
- Do not read hunched over a desk or table. Prop reading material at eye level.
- If you spend long periods on the phone at work or home, consider using a headset rather than cradling the phone between your head and shoulder.
- Don't crane your neck to see a poorly placed computer monitor. Place the monitor at eye level, square to your shoulders if possible.
- Ask your chiropractor to show you neck exercises to strengthen weak areas or relax tight muscles.
Neck pain that won't go away or keeps coming back can signal a more serious underlying problem such as a subluxation or degeneration in the joints (such as what occurs with arthritis). If you experience neck pain that doesn't abate within 24 hours, seek the advice of your chiropractor for diagnosis and treatment.


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Noah Van Hochman scouting the game trails.
Noah Van Hochman's unique, nonstandard perspectives on life, the universe and the outdoors are said to be due in part to the interesting cocktail of his massive intellect, boyish charm, fading good looks and of course, a colossal ego! With a philosophy and demeanor that seems to be more like equal parts of Doctor Evil and the Dalai Lama, rather than an Adventurer with an MBA, his alcohol-induced opinions will surely entertain and inform those who love their time under wild blue skies!


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Hardly Famous
Magician 19@nodkeem
Philosophical Model 19@nodkeem
The Art Colony and related sites are just a modern day book with photos
please share our address here with others as a book
and its a book by donation mostly yet we sell subscriptions to forums
other wise its free to view and listen to the music
REPRESSION is COMMUNISM! BODY AUTONOMY for ALL
In service to a great nation, I gave nearly my all. My soul for God and Country! Your Psychological Warrior for the war effort. MK-Donald/donna
While there are eternal truths such as gravity exists but why? (theory/model/faith) yet life is a series of half-truths that create an understanding or an illusion of preference for each of us when added up making what some call that faith theory or modeling.
My personal opinion is that I am HALF right, and you are HALF wrong.
DKMeek the Magician 19@nodkeem -
perhaps we are all conservatives yet we take the things that are offbeat and outcast at times and propose a better life that is more inclusive of the individuals in a society that is the liberal way
BILLS>H.R. 3611 (IH) - Kazakhstan Permanent Normal Trade Relations Act of 2023
Idiocracy would not relate Repression to Communism due to their years of training by the misdirecting repressors.
.Raydon. -
In my opinion, I am HALF right, and you are HALF wrong. Let's part ways. Each of us is taking our HALF LIFE in peace.Peach On Herb says Be Anointed with Kenah Bosum and seeking you will find HEAVEN is at hand.