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Let's announce the special winners of this week's Team Bingo Smackdown. All the teams fought the good fight, but only one team can prevail and take home the $3,000 CASH prize! Put your hands together for the following winners and don't forget to gather your team for next week's edition!
And The Winners Are…
Team Red Card Curies! captianlove2015, wellrounded, Ckmarks, tanyastar123joined their forces, and are the new online bingo champions! They battled until the end and they took home the $3,000 CASH prize!
If you missed this week's event and believe you have what it takes to battle it out for the grand $3,000 CASH prize, then join us every Thursday in our Fair and Square Room between 12AM and 10Pm EST!
Don't forget to register your team at [email protected] by Wednesday!
Have a great weekend!


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This is just a reference guide for myself really 🙂
CAS Morse Academic Plan – Core Classes
[X] Writing The Essay Fall 2009
[X] Conversations of the West Fall 2009
[X] World Cultures Spring 2010
[ ] Foreign Language (Up to the Intermediate II level)
[X] Spanish 3 Spring 2010
[ ] Spanish 4 Projected Fall 2010
[X] Course in the arts (Expressive Culture) English major overrides
[ ] Quantitative Reasoning
Major – English (10 courses/40 credits)
[X] Literary Interpretation (V41.0200) Spring 2010
[ ] British Literature I (V41.0210) Projected Fall 2010
[ ] British Literature II (V41.0220)
[ ] American Literature I (V41.0230) Projected Fall 2010
[ ] Theory of Drama (V41.0130)
[ ] Arthurian Legends ((V41.0717)
[ ] Senior Seminar – Dramatic Literature (V41.0971)
[ ] Dante & His World (V41.0143)
[ ] The Theory of the Avant-Garde, East and West (V41.0730)
[ ] Introduction to Old English Language and Literature (V41.0315)
Minor – Creative Writing (4 courses/16 credits)
[X] Creative Writing: Introduction to Fiction and Poetry (V39.0815) Spring 2010
[ ] Writers in Paris: Fiction or Poetry (V39.9818) (8 credit intensive)
[ ] Advanced Fiction Workshop (V39.0820)
Minor – Politics (5 courses/20 credits)
[ ] Quantitative Methods in Political Science (V53.0800)
[ ] Power and Politics in America (V53.0300)
[ ] Business and American Foreign Policy (V53.0736) pre-req: V53.0300
[ ] Political Parties (V53.0340) pre-req: V53.0300
[ ] The Politics of Administrative Law (V53.0354) Projected Fall 2010
On that note, I absolutely HATE my CAS advisor. Not only does she not know shit, but the (absolutely useless) seminar she encouraged that I take last semester, though it boosted by GPA, made no dent in my required courses. She knew I had enough AP credits to cover my electives, and should only be taking classes toward my major/minors, but instead of telling me to take another course in political science my first semester, she told me to take that seminar. And then this semester, I could have taken British Literature or American Literature (I) conjointly with my Literary Interpretation class, an option she didn't even tell me. Further, she told me (essentially) that I would do bad if I took both literary interpretation and creative writing in the same semester. Instead, the classes are so different (and don't have as heavy a workload as she implied), and I'm slated for A's in both (hopefully)
So basically, NYU advisors suck, just know your own shit tyvm. Now I'm going to cram and finish all of those classes by spring 2012. Just you guys wait and watch.
But thanks to idiot counselors who don't know what it means to be helpful to freshmen, I'll graduate with 132 credits instead of the 128 needed. One extra class, all for naught.
Hmmph. I'd do a better job as advisor than half of their current staff.
Trust me, I know my shit.

subset: commoncrawl


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Subscribe: Apple Podcasts | Android | Stitcher | TuneIn | Spotify | RSS | More
The media loves to write a good story with not much truth behind it. Anyone's claims are good enough as long as they fuel the agenda. But what happens when the truth is revealed and its time to admit an error? Usually, silence on behalf of the most powerful entity in the world. With their power comes no responsibility, or journalistic integrity, for that matter. Oh, and did we mention how much they love using the word "militarization" before "of the police?"
Al starts the show by continuing where he left off from the last show, by playing the Disco Dancer. Tracy recalls a story about a man who fabricated a story and a police report and ended up getting locked up. This story sparks a discussion about fabricated news and the crazy climate that we, as U.S. citizens, are in. Rocky briefly makes his return and attempts to sing We Are The World. Tracy then reads two articles which enraged him over the weekend. These stories discussed the militarization of the police and the pair taking the opposing side and talk about how blown out of proportion the two stories are.


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The visit of Egyptian Health Minister Hatem al-Gabali to Azerbaijan is currently being prepared, Trend reports quoting Agaselim Shukurov, senior councilor of Azeri Embassy to Egypt.
Shukurov said Hatem al-Gabali had been appointed Health Minister most recently. During his visit to Azerbaijan, he is going to speak of health protection in the two countries, in particular experts training, exchange of experience, fighting bird flu
and other infectious diseases.
Such visit had been scheduled yet last year. Egyptian ex-minister of health Avad Atjjuddin was planning to visit Azerbaijan during the year but resigned due to staff changes in the government.
A delegation of businessmen operating in health and pharmaceutics sphere is going to arrive in Azerbaijan alongside the minister.
Follow Trend on Telegram. Only most interesting and important news


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Cancer Daily Horoscope December 7 2016
A day of conclusions this Wednesday as some things from work become very clear and some sort of betrayal surfaces.
It may be that someone has done something against your wishes or against your own activities and you have just found out. Try to take a moment to really think everything trough so don't react in the heat of the moment.
Get daily your Cancer Horoscope here on The Horoscope!


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Well, hello! If you are new to our website, or if our website is new to you (we just reworked it, as you can see), let me enthusiastically greet you!
From time to time in this column (a term I prefer over "blog") I intend to write "pithy" (shades of Bill O'Reilly) articles that I hope will be of interest, and perhaps, even of help to you. While the emphasis in this website will be upon counseling matters, or those closely related to them, I don't intend to confine myself to such topics. I'll be writing about any number of things that interest me, and I hope will interest you too. So, here we go–off to a new start, with a new column and a new year. May the Lord bless you this year as you seek to serve Him.


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Mother Teresa
The Museum of Spirituality In Art in Franklin, Ohio commissioned this portrait of Mother Teresa. I made her hands big, a symbolic statement about the power of her life's deeds and of all the burdens she was able to carry. I was struck by how moving it was to think about her as I worked on the portrait.
Clay for bronze.
22 x 11 x 10 inches.


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Writing oneself into letting go - I don't think so
During a good discussion last night with a couple of my friends about the way I am choosing to deal with this we covered some of the ground of recent posts. In particular, we discussed the pros and cons of such public introspection. If anything, I became more convinced that it has more benefits than costs. One friend agreed that the act of writing does influence how one thinks about things. He asked me to consider whether I could write myself into a decision to let go.
This is an interesting question but at present, I am somewhat sceptical about this being possible. The problem is that once I have let go, writing anything at all ceases to make sense. It seems unlikely that one could write something so convincing that all further writing becomes pointless.
I know I can't write myself towards my desired state - reconciliation with her. I think writing does help to moderate my emotions towards a stable, if not particularly desirable state. I just don't think I can get to an emotionally empty state which is what letting go looks like from here.
Yes, people will tell me that in that state I will be free to enjoy other things in life. There is a point to that, well illustrated by "The Cat Empire" gig I went to on Tuesday.
The Cat Empire have a very energetic style of music and performance. The Metro was packed and everyone - and I mean everyone, was bouncing up and down to the music like there was no tomorrow. Introspective little me didn't actually feel like dancing so I went and stood against a wall so that I wouldn't get jumped on and observed the concert from there while I reflected on the day's events. It must have looked strange to those around me, but not dancing by the side wall seemed less obvious than not dancing in the mosh pit.
But that was Tuesday, near the centre of a recent storm. I know I will get past that and I will dance again - probably as soon as next Friday at the Mammal gig - "Hell Yeah!"


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To the Editor:
Our cluster group, Friends of Refugees in the Champlain Valley, in association with the North Country Refugee Collaboration Project, was considerably encouraged with your support of our mission ("Taking in refugees will be good for the Adirondack Park").
We have made contact with the Vermont Resettlement Program which has been successfully integrating migrants for 25 years.
Secretary of State John Kerry has just publicly declared that ISIS is committing acts of genocide. Of the 20 million citizens of Syria, 10 million are seeking safe asylum.
They have no more homes in Syria; they, in fact, have no more country to return to. Europe is facing destabilization and needs the rest of humanity to reach out.
Those "Others," those strangers with no assets, no papers, no recommendations, could be us. It's hard to imagine there being no Red Cross, no federal disaster assistance, no schools for shelters, no hospitals, no cars, radios, cell phones, blankets, pharmacies — no nothing. As Scrooge famously asked in the Christmas Carol, "Are there no work houses?"
No, not even those.
This is not just a Mideast, or European crisis. This is a global disaster that needs us all to give concrete suggestions and help and yes, perhaps even shelter. Shunning isn't a solution, it is a punishment and there refugees have had enough.
When we see in their faces our own beloved families, then it is harder to turn away.
Bobbi Perez


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Re: ADAMS of Halifax County
In reply to:
Re: ADAMS of Halifax County
Jim Sawyer 7/20/01
My genealogy program, Reunion for Macintosh, says it can import GedCom files from Windows files. I've never tried importing files, though I'd like to try. It is worth a try, don't you think?
By the way, I am also descended from a long line of Sawyers: Alabama Sawyer (Alla) < Henry Isaac Sawyer < William Early (Buck) Sawyer < George Sawyer < John Sawyer, Sr. < William Sawyer II < William Sawyer I.


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On TV this Valentine's Day: The Amazing Race expands its palette, Versace has a big announcement and grown-ish learns the downside of social media. Here are 11 programs to keep on your radar:
Courtesy of CBS
On TV this Valentine's Day: The Amazing Race expands its palette, Versace has a big announcement and grown-ish learns the downside of social media. Here are 11 programs to keep on your radar:
Series premiere (all eight episodes): Deaf actors Josh Feldman and Shoshannah Stern (Jericho) created and star in this half-hour dramedy about best friends, one of whom is newly engaged and the other is trying to get over an ex; Zach Gilford (Friday Night Lights), Cheryl Hines (Curb Your Enthusiasm), Colt Prattes (ABC's Dirty Dancing) and Marlee Matlin (Switched at Birth) recur.
The comedian's first special in 10 years touches on contemporary issues involving relationships, race and society. (Watch trailer.)
Sarah joins Eddie in Paris, where he is set to open Europe's first Meyerist Center; Sarah's search for Lilith reaches a point of no return.
Events include figure skating (pairs' gold medal final), alpine skiing (men's Super-G gold medal final) and women's speed skating (1000m gold medal final).
Comedians including Kevin James and Whitney Cummings share their thoughts on love, dating and relationships. (The Top 14 Greatest Valentine's Day Movies of All Time, hosted by Dean Cain, follows at 9.)
Zoey's social media activity threatens to derail her dream fellowship at Teen Vogue. (Jam out with our grown-ish playlist.)
Two hours: A contestant misplaces their passport in Bahrain; the racers head to Thailand, where they chow down on scorpions (not a crossover).
Quentin faces off against an intimidating adversary; Julia assists Alice with a dangerous endeavor.
Celebs include American Gods' Orlando Jones, Arrested Development's Judy Greer, SNL's Colin Quinn and Abby Elliott, 30 Rock's Judah Friedlander and Sabrina's Caroline Rhea.
79 minutes: Naval Officer Jeffrey Trail meets Andrew Cunanan; Versace comes out to the world. (Fall behind? Reap Ep. 4 recap.)
The Branch Davidians refuse to surrender; David Koresh waits for a sign from God. (Who was Performer of the Week?)


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Planet Labs' GeoTIFFs can be used in Google Earth, but the georeferencing metadata will only be detected by Google Earth Pro. Planet's analytic and visual scenes load in Google Earth Pro without any issue, but there is an incompatibility in Google Earth with the EPSG:3857 spatial reference system used in Planet's mosaic quads. Unfortunately, Google Earth is only able to recognize GeoTIFFs that have declared the EPSG:900913 in the image metadata even though EPSG:3857 and EPSG:900913 are equivalent codes.
Fortunately, there is an easy fix with GDAL:
gdalwarp -t_srs EPSG:900913 -co "COMPRESS=DEFLATE" -co "INTERLEAVE=PIXEL" mosaic.tif fixed_mosaic.tif
With EPSG:900913 in the GeoTIFF header, Google Earth will be able to correctly geolocate the image on the globe.


text: BEST PHOTO BOOTH.EVER SHALYN & SCOTTIE My fiance Scott and I have already been together for 13 years, he said he "didn't believe in marriage". But, we have a great relationship with a house and a dog and were living very happy lives together as life partners. As his 40th birthday approached we planned a big party and golf event with 40 family and close friends. I had no idea that it would turn into an engagement party... While he was giving his little speech thanking all the people in his life that got him this far he pretended he forgot to thank me (always trying to be a funny guy) and then got down on one and asked me to marry him in front of everyone. I couldn't have been more shocked! So obviously I said yes (through all my ugly crying) and now we are planning a wedding at the Now Sapphire this January!!!


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My mom and I decided to take a little trip this year together. It's been a long time! We set off at the end of August for California to do a mommy & me vaca.
First we flew down to Santa Barbara to spend a few days milling around downtown for shopping and food, with a little visit to the coast. We did meet up with one of my dearest gal-pals, her hubby and her mom for dinner as well. I was not thoughtful enough to take photos of us all, but not to worry...they met up with us at our next destination!
We were lucky enough to land in one of mom's World Mark Condos in Solvang. Solvang was a cute Danish village a little over a half hour from Santa Barbara. The condo was a big 2 bed/ 2 bath with a generous living/dining area. We even lucked out with the wheelchair accessable room, which meant even more space! There is a lot of yummy Danish food, shopping and wine tasting to be done there. We spent time with Kerry, Kevin and Debbie one evening just tasting the yummy local wines of the area. As everyone knows, CA is filled with vinyards! We even stopped by the Blackjack Ranch where they filmed the movie Sideways.
After a few days down south, we headed up the coast toward Santa Cruz to see my mom's cousin Litsa and her family. This was just a short stop to grab dinner, but it was really nice to finally meet her! We even met a few furry friends on the way!
That evening, we trudged our way to San Francisco, both tired and stiff from a long drive. We got to the next condo (more like a hotel room) late so we didn't start our tour of San Fran until the next day. The days flew by as we both walked and rode our way around the many faces of the big city. We didn't rent a car for this portion of the trip so we got a pass for the trolly and saw as much as we could over the next four days. We even got to visit with another of my friends, Corinne, who had moved there a few years ago.
There were just too many things to tell you about, so I decided to just post a couple photos and leave it at that. I would be happy to chat about the places we went over lunch if anyone is so inclined, but that will be it for my blog post. I will close by saying that I had a great time and was happy I got to spend some much needed one-on-one time with my oh-so-wonderful mother!


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Raku Fired Sculptural Vase
'Pisces' Vase – gold, pink, copper matt
Collect from gallery £205
Including UK delivery 220
H 39 cm
Raku glaze and copper matt fumed stoneware. Sculptural slab built forms and thrown work.
'I work with copper matt fumed Raku, this is an erratic and unique technique which is notoriously difficult to master! However this is also part of its' elusive charm and ensures that each pot is a unique work.
My own work is sculptural in form and has architectural and sculptural qualities. Ideas originate through drawing, and initially I was inspired by the work of Richard Serra, and painters such as Juan Gris, and William Scott.
The forms developed from the Jug shape which was my take on the still life- and became a sort of totemic or non functional form. (some might say pointless!)
At University I was introduced to copper matt fuming in the John Wheeldon style. This lent the work a painterly and atmospheric quality. I have adapted the process to use on larger forms, and whilst still frustratingly unpredictable -it has become a source of endless experimentation, rather like searching for the lost chord or panning for gold!


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Speargrass Inn has now extended its country fare menu to include cottage preserves. Our homemade jams, chutneys and preserves are used throughout our menu and a great take home gift for your pantry or as a gift.
A taste of whats on offer:
Vinaigrettes - Pear and Riesling
Balsamic Range - Balsamic Glaze & Cherry Balsamic
Chutney Range - Apricot, Red Onion Marmalade, Tomato Chili Jam
Sweet Jam Range -Mixed Berry, Apricot
Sauces - Stonefruit BBQ Sauce, Plum Sauce, Apricot Sauce
Spring hours: Cafe open 8.30am - 4pm. Open late Fridays for evening dining. Closed Tues & Wed.
PUBLIC HOLIDAYS: if fall on opening days above, no surcharge will apply.


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4minute's sub-unit, 2YOON, is gearing up for their debut and has now released the music video teaser for their upcoming song '24/7′.
2YOON is consisted of two members of 4minute: Gayoon and Jiyoon. Their upcoming debut album, 'Harvest Moon', is said to be the very first Korean country pop album. It will be released on January 17th, so stay tuned!
Check out the teaser below.
Source: allkpop
Article by Karolina & Alona
- Excited
- Fascinated
- Amused
- Bored
- Sad
- Angry


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The Center for O & P Care is full-service orthotic and prosthetic firm. Our board certified practitioners evaluate, customize and fit prostheses for all levels of amputation. Our board certified orthotists customize and fit supportive or corrective devices for patients with impaired neuromuscular or skeletal systems. Our facilities are fully accredited by the American Board for Certification in Orthotics and Prosthetics (ABC).
We offer pre- and post-surgery, and rehabilitation services including patient evaluation, custom fitting programs, and coordination with the physicians office and the hospital. We follow through with managing the rehabilitation process, customized for each patientís individual needs.
To ensure that you have the best device for your situation, COPC employs advanced technology. Our clinicians continue to stay abreast of the latest in industry advances by attending board certified continuing education sessions and seminars, giving them the expertise to provide any type of orthotic or prosthetic device.
Why Center for O & P?


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The second component of Lotus Development Corp.'s knowledge management system code-named Raven has been delayed again.
The company confirmed today it will delay the final release of its Discovery Server by one month to improve some of its profiling abilities.
In January, Lotus CEO Al Zollar promised the server would ship in March, saying, "I know this product is overdue."
Scott Cooper, senior vice president of knowledge management at Lotus said, "It's not bugs and the scalability. It's how well we build expertise profiles."
The problem wasn't foreseeable in the laboratory, he said, but only became apparent after beta users tested the product. The server searches a company intranet to find information in collaborative applications that will let the user locate files and experts in certain fields and share information instantly with colleagues, among other things. The more a user acquires a certain kind of information, the more "expert" he becomes and thus a resource.
"We found down the road that real-life users were associated with categories they shouldn't be," Cooper said.
So, Lotus developers are back to work creating the algorithms that will correctly match experts with their fields of expertise.
The beta testing will continue until the release of Discovery Server gold code on April 30, Cooper said.
The Discovery Server sorts data for users to access and is meant to work with the K-station portal builder, which was released in December. Together, they form Lotus' knowledge management system, known as the Knowledge Discovery System.
Cooper said implementing the gold code for those who have already installed the beta version shouldn't be difficult and only involves one relatively small build on the existing server software.


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SOUTH BEND -- A 38-year-old man was sentenced Friday to 55 years in prison for killing his wife last summer, the maximum allowed under the terms of his plea agreement.
Brian Cocot pleaded guilty on Sept. 27 to one count of murder in connection with the death of his wife, Marsha Cocot, who was found strangled to death in her home on June 13.
Cocot had planned to kill himself in Elkhart after killing his wife, according to court documents. But when his suicide attempt failed, he told police to check on his wife.
Cocot's plea agreement with the state called for the judge to sentence him to no more than 55 years in prison.
The maximum sentence for a murder conviction under Indiana law is 65 years.


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When one wants to feel special or look their very best, we put in hours and hours of efforts to make that happen. Spa treatments, visits to the beauty parlors, a new haircut, experimenting with beauty and makeup products and jewelry are essential steps of this process. No matter how tiring and time consuming it is, we don't mind as eventually we are absolutely impressed with the outcome. But what happens when there's an event around the corner and your pet is also invited. You would want it to look just as glamorous as you do. Of course you will try to make do with the tools available at hand, and it could be enough.
But for special occasions you need special care, so does your pet. Over the past few years there has been a rising trend across the world with pet salons catering to your little friend's every need sprouting up. One only needs to refer to the exhaustive list of services provided by these salons, zero in on the requirements as per your wishes, and leave it up to the professionals to take care of the rest. This pampering does come at a price, but these adorable creatures are totally worth it. The services offered range from the most basic hygienic purposes to catering to your pet's every glamorous requirement.
There are cases where a pet owner is unaware of some of the most basic hygienic demands that are essential for its well being. Or sometimes he is unable to allocate sufficient time for the same. In some cases, the pet becomes unusually aggressive when the owner tries to take care of it. Pet salons come to the rescue in such situations. Cleaning of teeth, gums, ears and eyes along with nail clipping are crucial for the pet's health. The professionals are equipped with the necessary tools. They also have the required knowledge to take care of your pet, and to control it in case of aggressive behavior.
These salons also offer a variety of pet health services. If your pet is infested with ticks and fleas, or there is something wrong with their skin or fur, apart from a veterinary doctor, these pet salons can also prove to be helpful. Flea baths, proper cleansing routines, provision of repellants and skin lotions are some of the services that cater to your pet's health requirements. At the same time, one must make sure that the professionals are updated about any probable allergic reactions. For a busy pet owner, pet salons are a boon.
After a long tiring day, a visit to the spa is heavenly. It calms your mind, soothes your nerves and prepares you for anything that is about to happen next. Similarly, a pet spa has a similar if not greater effect on your pet. It relaxes your pet and also gives you time to run some errands in the meantime. The services offered in pet spas are also varied. Specific treatments can be designed according to your requirements or your pet's characteristics. Some spas also have services that aid in the general well being of your pet, in case there is a health defect in the picture.
Pet salons venture into the field of focusing exclusively on your pet's physical appearance. Unique styling of your pet's hair, a different hair cut, experimenting with application of human hairstyles in your pets such as Mohawks or even hair color are some of the services one can avail if one wishes to give a unique look to their beloved pet. The pet distinctly stands out, in such cases. Or if one wishes to remain rather subtle, it gives the pet's appearance a clean finesse instead of rugged.
Certain pet salons provide owners with costumes fitted to the pet's requirements. In events involving a pet's participation, such as fancy dress or Halloween, the pet becomes the cutest most talked about entity. Pet salons also provide the owners with a selection of the most popular toys and accessories that can be used at such events or for daily use. One can also buy tools that can help you in pampering your pet, in the comforts of your own home. The right kinds of toys, hairbrushes, bathing soap, food, etc. are available at one's disposal, that are suitable for your pet based on its size, age and breed.
Not only that, some salons also hold training lessons for the pet owners to keep them informed about the host of options available to them. This also increases awareness among them about the right way to take care of your pet such as in terms of grooming requirements. The rising expectation of pet owners to have the best pet is fuelling the growing demand for such establishments that cater exclusively to all kinds of pets. Pet salons are especially useful, when intends their pet to be a part of pet shows or if the pet has been chosen to appear in the media, such as advertisements, television shows or movies. So go ahead, pamper your pet, and be the centre of your pet's attention and that of everyone else.


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It's in limited supply but it's one of the best buys on the Australian wine landscape. Especially from a year like 2017. Is it Cellar Door only? It may well be.
Tasted : Aug17
Alcohol : 13.7%
Price : $28
Closure : Screwcap
Drink : 2018 - 2024
Visit winery website
Add to My List


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The Bellamy Law Firm is pleased to announce that attorney David J. Gundling has joined its practice. Gundling's former firm, The Gundling Law Firm, will now operate as a new branch of the The Bellamy Law Firm in Pawleys Island. With the strong continued ties to the community that The Gundling Law Firm has established, The Bellamy Law Firm now brings over 50 years of expertise to the Pawleys Island area - providing preeminent representation in over a dozen legal practice areas across the entire Grand Strand.
Myrtle Beach Office: (843) 448.2400
1000 29th Avenue North, Myrtle Beach, SC 29577
Pawleys Island Office: (843) 237.3400
239 Business Center Dr., Pawleys Island, SC 29585


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Located in Melbourne, Victoria Abn: 45781585012
Features & Construction Standards
Construction Standards (EASTERN EDITION)
Aggressive Front Profile for Increased Towability
Aluminum Framed Front, Rear and Side Walls
Aluminum Framed Slideouts
Laminated Fiberglass Exterior Side Walls
2'' Thick Laminated Aluminum Floor
Darco Moisture Barrier
One Piece TPO Roof
E-Z Lube Dexter Axles
Electric Brake System (4wheels)
R-11 Insulation in Floor
R-7 Insulation in Sidewalls
R-11 Insulation in Roof
In-Frame Fresh, Grey, and Black Water Tanks
7.5'' - 10.5'' Steel Z-Beam with Full-Length Welded Outrigger
1/4'' ABS Enclosed Underbelly
Exterior Camping Package (EASTERN EDITION)
6 Gallon Gas/Electric DSI Water Heater with Quick Recovery
Radius Entry Door with Gas Strut
Outside Speakers
4 Stabilizer Jacks
TV Antenna & Cable/Satellite Hook-Up
Radial Tires
Electric Awning
LED Awning Light
Wide Stance E-Z Lube Dexter Axles
Outside Shower (Hot & Cold Water)
Black Tank Flush
Exterior Features (EASTERN EDITION)
Dual Propane Tanks with Cover
In Frame Dual Battery Rack
Spare Tire
Extended 4'' Down Spouts
Front Galvanized Steel Rock Guard
Oversized Grab Handle (main door)
9" Steel Triple Entry Steps
Magnetic Storage Door Catches
Aluminum Wheels
Power Tongue Jack
Oversized Exterior Pass Thru Storage with Lighting
Fiberglass Front Cap
LP Quick Connect
Outdoor Kitchen (243BHS, 257RSS, 261RBS, 272BHS, 287QBS, 308BHS, 330BHS)
Interior Camping Package (EASTERN EDITION)
Night Shades
Bluetooth Stereo with Aux and I-Pod Hookup
Hardwood Cabinet Doors
Foot Flush Toilet
Tub Surround with Shelves
Full Extension Drawer Glides
6 cu./ft. Double Door Refrigerator
Stainless Steel Sink
Flush Mount 3 Burner Range with Glass Cover
Interior Features (EASTERN EDITION)
Residential Style Furniture
Night Shades in Living Area
Water Heater-By-Pass for Winterization
Interior LED Lights
17'' Oven
Towel Hooks
Power Vent Fan
Solid Wood Medicine Cabinet
Linen Storage
Deluxe Curved Shower Curtain
Master Bedroom (EASTERN EDITION)
Upgraded Foam Mattress
Dual 110V Outlets
Mirrored Wardrobes
Under Bed Storage
Individual Reading Lights
Hardwood Entry Doors
Laundry Chute
Safety Equipment (EASTERN EDITION)
Carbon Monoxide Detector
Smoke Alarm
Fire Extinguisher
Electric Brakes
Safety Chains
Frameless Tinted Safety Glass Windows, Egress Windows (Fire Escape)
GFI Recepticals
Thermal Package (EASTERN EDITION)
Ducted Heat and Enclosed Heated Underbelly
2" Duct off of Furnace to Underbelly
30K BTU Furnace and Residential Heat Ducts
3 Year Limited Structural Warranty
Enjoy peace of mind with the Industry's Best Three Year Limited Structural Warranty.
Wired For Performance
The only towable manufacture to incorporate color-coded and numbered electrical wiring on all products
Supercharged/A/C Cooling
Tuf-Lok thermoplastic couplers form a permanent, crush-resistant seal in your air-conditioning duct.
In-Floor Residential Heat Ducts
Residential in-floor heat-duct system just like in your home.
110 RVDA Quality Circle Awards
Keystone brands have earned 110DSI Quality Circle Awards since 2007.
7 Layers of Protection
Most caps are fully painted and clear-coated in our in-house state of the art paint facility.
Tru-Fit Technology
Tru-Fit Five-Point slide room construction backed by the Industry's Best 3-Year Limited Structural Warranty.
Best-In-Industry 4 Stage Quality Process
Step 1. Engineering and testing
Step 2. In-Progress inspections and audits
Step 3. 120- Point Secondary PDI
Step 4. Shipping department review
We only sell the best Caravans and 5th Wheelers, manufactured by the Top Companies in the USA.
Our Customers get the Best Caravan or 5th Wheeler at the Best Price.


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Track Pad Market to Witness Widespread Expansion During 2018 to 2028
Track Pad Market: Overview
A track pad or a touch pad or a glide pad is an input pointing device having a specialized surface that helps translate the motion and position of a user's finger to a relative position on the screen of the device. Track pads are considered as one of the best alternatives for pointing devices such as portable media players and mouse in laptops where space limitation is a concern.
Globally, track pads have been conventionally featured in laptops and computers, but now they can be purchased separately as portable devices as well. A track pad contains a grid of cables called transmitting and receiving cables, which are capable of recognizing the touch of a finger on the screen and transferring the signal through cables, resulting in the movement of the cursor on screen.
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Furthermore, track pads are also used for navigating computers from a couch when connected to the TV. Apple Magic, Lenovo Wireless, and Perixx PERIPAD-501 are some of the popular track pads available in the market. Furthermore, track pads can detect a varied range of gestures such as clicking, zooming and scrolling. The selection of the track pad depends on gesture, application and cost, among other factors.
Track Pad Market: Dynamics
The major factors driving the growth of the global track pad market include the high resistance of track pads to dirt, surface scratches and moisture. Track pads can be considered as the most efficient pointing devices if portability and space are the prime criteria as they can be connected via Bluetooth or wireless technologies, which in turn acts as a driver for the growth of the track pad market globally. Moreover, the requirement of slight motion and no pressure increase the durability of track pads.
Limitations on the devices that can use track pads and the sensitivity of track pads are some of the factors hindering the growth of the track pad market globally. Other factors hampering the growth of the track pad market globally include the less possibility of heavy movement and insensibility to gloved fingers and pencil tips. Furthermore, the limited area provided for the motion of fingers and scrolling problems are some other factors restraining the market growth. Sometimes, the sensitivity of the track pad may prove to be a problem for some users. Also, for operations that require precise control use of track pad becomes little difficult these all things restrain the growth of global track pad market.
The integration of double-finger scrolling track pads in laptops makes working on a track pad easier and more interesting. Furthermore, track pads now come equipped with features such as tap-to-click, zoom-in or -out, rotation and smart swiping may further surge the demand and sales of the product.
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Track Pad Market: Region-Wise Outlook
Japan and North America are the two prominent regions estimated to account for dominant shares in the global track pad market due to the presence of recognized manufacturers and the growing pace of technological advancements in these regions. Europe as well as Asia pacific are expected to witness substantial growth during the forecast period, owing to the growing adoption and awareness amongst consumers towards choosing advanced products. Moreover, growth of the sales network and mergers of prominent manufacturers with local distributors in the Middle East and Africa region may contribute towards the growth of the market in the region.
Track Pad Market: Market Participants
Examples of some of the market participants identified across the value chain of the global track pad market include,
- Apple Inc.
- Lenovo
- Wacom
- HP Development Company, L.P.
- Synaptic
- Elan Tech
- Cirque Corporation
- Alps Electric Corporation
- Logitech
- Panasonic Corporation
This analytical research study imparts an all-inclusive assessment on the market, while propounding historical intelligence, actionable insights, and industry-validated & statistically-upheld market forecast. Verified and suitable set of assumptions and methodology has been leveraged for developing this comprehensive study. Information and analysis on key market segments incorporated in the report has been delivered in weighted chapters. A thorough analysis has been offered by the report on
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Compilation of authentic and first-hand intelligence, insights offered in the report are based on quantitative and qualitative assessment by leading industry experts, and inputs from opinion leaders & industry participants around the value chain. Growth determinants, macroeconomic indicators, and parent market trends have been scrutinized and delivered, coupled with the market attractiveness for each market segment encompassed. Qualitative impact of growth influencers on the market segments across regions has also been mapped by the report.


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The cold demon and LG are buddies; they meet often and forge a friendship that's difficult to break. I'm working on building his immunity and consciously stop administering allopathic medicines immediately. On a recent visit to his paediatrician, I noticed the vaccine board had Flu vaccine listed under the advanced section. I checked with the Paed and he advised I could go ahead with it. Vaxigrip, the flu vaccine can apparently be administered to babies above 6 months of age.
Wednesday, February 06, 2008
Vaxigrip – Flu Vaccine
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The College of Business welcomes Marissa Mainwood as student engagement coordinator, a newly created position designed to increase student participation in college and campus-wide programs that strengthen leadership skills and enhance career prospects.
With an enrollment of 6,360 undergraduate and graduate students – making it one of the largest business schools in the nation – the college wants to ensure all students are taking advantage of the numerous opportunities available to them at Florida State University, including professional development workshops, industry immersions, case competitions, internship and job-placement services, alumni mentorships and networking opportunities.
Mainwood, who reports to Marissa Langston, assistant dean for alumni and community engagement, comes to the college from Tallahassee Community College (TCC), where she most recently served as a data analyst and special projects coordinator overseeing TCC Leadership Institute programming. She earned her Master of Business Administration (MBA) from Florida Atlantic University and her bachelor's degree in psychology from Florida State.
"While academics are a priority, we view activities to learn leadership and soft skills as essential to producing well-rounded graduates grounded in their various fields and prepared to thrive in their careers," Langston said. "With Marissa's hiring, we have a great opportunity to expand and strengthen our efforts."
Mainwood will work toward increased student involvement in student networking and engagement offerings, such as the Local Business Shadowing Program, Lunch with a Rotarian Program, the Professional Mentorship Program, the MBA Shadow Program and 'Noles on Wall Street, among others. Encouraging participation in college's 30 clubs and organizations through which students can hone their specific industry skills and build networks also is on Mainwood's agenda.
Additionally, Mainwood will assist in the development and promotion of diversity and inclusion activities within the college These events, including specific days focused on leadership and diversity, will give students the opportunity to explore these complex issues before entering the workforce.
Mainwood also is charged with encouraging a culture of giving among students, preparing them to give back to the college as they advance in their careers.
To learn more, contact:
Marissa Mainwood
Student Engagement Coordinator
By Barbara Ash


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CD36 is a ubiquitous membrane glycoprotein that binds long-chain fatty acids. The presence of a functional CD36 is required for the induction of satiety by a lipid load and its role as a lipid receptor driving cellular signal has recently been demonstrated. Our project aimed to further explore the role of intestinal CD36 in the regulation of food intake. Duodenal infusions of vehicle or sulfo-N-succinimidyl-oleate (SSO) was performed prior to acute infusions of saline or Intralipid (IL) in mice. Infusion of minute quantities of IL induced a decrease in food intake (FI) compared to saline. Infusion of SSO had the same effect but no additive inhibitory effect was observed in presence of IL. No IL- or SSO-mediated satiety occurred in CD36-null mice. To determine whether the CD36-mediated hypophagic effect of lipids was maintained in animals fed a satietogen diet, mice were subjected to a High-Protein diet (HPD). Concomitantly with the satiety effect, a rise in intestinal CD36 gene expression was observed. No satiety effect occurred in CD36-null mice. HPD-fed WT mice showed a diminished FI compared to control mice, after saline duodenal infusion. But there was no further decrease after lipid infusion. The lipid-induced decrease in FI observed on control mice was accompanied by a rise in jejunal oleylethanolamide (OEA). Its level was higher in HPD-fed mice than in controls after saline infusion and was not changed by lipids. Overall, we demonstrate that lipid binding to intestinal CD36 is sufficient to produce a satiety effect. Moreover, it could participate in the satiety effect induced by HPD. Intestine can modulate FI by several mechanisms including an increase in OEA production and CD36 gene expression. Furthermore, intestine of mice adapted to HPD have a diminished capacity to modulate their food intake in response to dietary lipids.
Citation: Naville D, Duchampt A, Vigier M, Oursel D, Lessire R, Poirier H, et al. (2012) Link between Intestinal CD36 Ligand Binding and Satiety Induced by a High Protein Diet in Mice. PLoS ONE 7(1): e30686. https://doi.org/10.1371/journal.pone.0030686
Editor: David Holowka, Cornell University, United States of America
Received: September 7, 2011; Accepted: December 21, 2011; Published: January 25, 2012
Copyright: © 2012 Naville et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research project was supported by Agence Nationale pour la Recherche (ANR-07-PNRA-015, SensoFAT project) and by the "Taste, Nutrition, Health" cluster Vitagora. AD is the recipient of subsides from ANR. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
CD36 is a multifunctional plasma membrane glycoprotein expressed by a broad variety of tissues [1], [2]. This receptor-like protein is able to bind multiple compounds such as oxidized LDL [3], collagen [4] or thrombospondin [5]. It also displays a very high affinity (i.e. in nanomolar range) for long-chain fatty acids (LCFA). Despite its multifunctionality, CD36 generally plays a specific role in a given cell type. For example, it is involved in collagen-mediated cytoadhesion in platelets, whereas it mediates LCFA uptake in myocytes. This cell specificity of function probably results from both cellular context (genotype and microenvironment) and aspects of CD36 itself. Indeed, regulation of the gene encoding this receptor is unusually complex [6] and this protein is subject to several post-translational modifications in a tissue-specific manner [7]. Recently, CD36 has been identified in rodent gustatory papillae as a lipid sensor involved in both the preference for fatty foods and cephalic phase of the digestion [8], [9]. CD36 is also found in the small intestine, in which its mRNA and protein levels are higher in the proximal parts (duodenum and jejunum), the main sites of fat absorption [10], [11]. Moreover, its localization is strictly restricted to the brush border membrane of differentiated enterocytes [10], [12], [13]. Although this location raises the possibility of an involvement in lipid uptake by intestinal cells, the specific role of CD36 in the small intestine is not yet fully understood. If comparison between wild-type and CD36-null mice has clearly shown an implication of CD36 in the formation, secretion and clearance of intestinal lipoproteins [14], [15], its involvement in FA uptake remains more elusive [16]. Indeed, some investigators report that there is no alteration of global LCFA absorption in Cd36-invalidated mice [13], [16], while others suggest that CD36 plays a role in LCFA uptake in the duodenum and proximal jejunum, but not the distal intestine [17]. Alternatively, it has been demonstrated that intestinal CD36 exerts also a role of lipid receptor, driving cellular signals stimulating chylomicron synthesis [13].
It is known that a lipid infusion into the intestinal lumen leads to a decrease in food intake [18] through mechanism which remains to be clarified. It was recently shown that this phenomenon could be in part related to the generation/mobilization of the lipid messenger oleoylethanolamide (OEA) by the jejunum mucosa, specifically in response to dietary lipids [19]. Using mice displaying a total invalidation of the Cd36 gene, Schwartz et al have reported that hypophagic effect of dietary fat requires the presence of CD36, because CD36-null mice are insensitive to the feeding inhibitory effects of duodenal lipid infusion [19]. They conclude that intestinal CD36, by facilitating oleate uptake as a precursor of OEA and/or its synthesis could raise the production of OEA. According to our recent data [13], an involvement of CD36 as lipid receptor at the origin of cell signalling, is an alternative hypothesis explaining its effect on OEA quantity in jejuna mucosa [19].
To further explore the role of intestinal CD36 in the regulation of feeding behaviour, impact of an acute duodenal infusion of nutritional (lipids) and/or pharmacological (sulfo-N-succinimidyl oleate, SSO) CD36 ligands on the regulation of food intake was studied in wild-type and CD36-null mice. High-protein diets (HPD) are known to induce a prolonged decrease in food intake [20], [21]. To assess the relative anorexic effect of dietary lipids, we next explored whether the hypophagic effect of lipids was identical in mice subjected to a standard (control) or a HP diet.
Materials and Methods
All procedures were performed with the approval of the Regional Committee of Ethics for Animal Experiments.
Wild-type (Harlan, Le Marcoulet, France) and CD36 null male C57Bl/6J [22] mice were housed in a ventilated cabinet at 21°C with inverted light/dark cycle (darkness from 1:30 pm to 1:30 am) and free access to water and standard chow (A04, SAFE, Augy, France). For the intraduodenal infusion experiments, 5-week old mice were fasted for 6 hours and anesthetized using isoflurane throughout the surgical procedure. A silicone catheter (0.31 mm, inner diameter) was placed in the duodenum through a small incision and fastened using surgical adhesive (3M Vetbond tissue adhesive, Centravet, Lapalisse, France). The catheter was threaded under the skin to exit through an incision in the back of the neck. Duodenal catheters were flushed every day with a mixture of polyvinylpyrrolidone and marbocyl to avoid clogging of the catheter, mixture supplemented every two days with ketoprofen. Mice were allowed to recover for one week after surgery. Catheterization allowed us to infuse solutions directly into the duodenum of awake mice, after a 5-hour daytime fast.
Duodenal infusions
Experiment 1: Effect of a lipid infusion on short-term food intake.
After surgery, control mice were maintained on standard chow and infused, in a first series of experiments, with either isotonic saline solution or 20% Intralipid emulsion (IL from Fresenius Kabi France, Sèvres. It contains 20% soybean oil, 1.2% egg yolk phospholipids and 2.25% glycerine in water), at a rate of 6 µL/min during 10 min (0.12 Kcal infused). The end of the infusion/refeeding corresponds to the beginning of the dark period when mice were disconnected from the infusion pump. Food intake (one pellet of standard chow was weighed immediately before and after the test) was measured 30 min, 1 and 2 hours after the end of the infusion (Fig 1). Each animal was tested for each treatment condition alternatively, which means that each animal was its own control. Following feeding tests, mice were killed by cervical dislocation 45 min after the end of one type of experimental perfusion and different parts of the small intestine as well as total hypothalamus were recovered.
Experiment 2: Effect of sulfo-N-succinimidyl oleate on food intake measured after saline or Intralipid perfusion.
In a second series of experiments, control and CD36-null mice (kind gift of Dr. Maria Febbraio from Lerner Research Institute, Cleveland, OH, USA) were infused prior to the saline and IL infusions, with either 2-pyrrolidone, Pyrr (vehicle) or sulfo-N-succinimidyl oleate, SSO (20 µM solution infused at a rate of 3 µL/min during 5 min). To avoid the infusion of dimethylsulfoxide, DMSO (the classical solvent of SSO), we used 2-pyrrolidone which has the advantage to make SSO soluble (F. Falson, personal communication) and to be miscible in water. The same mouse was alternatively exposed to the different infusions: pre-infusion of either 2-pyrrolidone or SSO followed by either saline or IL infusion (Fig 1). SSO specifically binds to CD36 and arrests the LCFA transport into adipocytes [1]. It was used in these experiments to specifically inhibit CD36 present in intestine cells. Food intake was measured as above.
Experiment 3: High-protein diet and lipid sensing.
Five-week old male wild-type and CD36 null male C57Bl/6J mice were maintained either on standard chow or on an isocaloric protein-enriched diet, HPD (SAFE, 54% protein versus 16.1% in standard chow) up to 30 days (Table 1). Weight and food intake were recorded every day. Food intake was expressed as grams of food consumed by gram of body weight to obviate any differences in body weight between mice. Three groups of wild-type HPD mice were separated and maintained on HP diet for 4, 12 and 30 days. At the end of the experiments, mice were killed by cervical dislocation after a 6h-fast. Blood and different parts of the small intestine were recovered either as total tissue (RNA preparation) or the mucosa was scraped off with a spatula (proteins), frozen immediately in liquid nitrogen and stored at −80°C until used.
For infusion experiments, surgery was performed as described above on control or 12 day-HPD fed mice of same age. NaCl and IL perfusions were performed as above, each animal being its own control. HPD mice were fed the same HPD food before and after the infusion experiments. As above, mice were divided into different experimental groups for analyses of gene expression alteration elicited by one type of experimental infusion. Duodenum and the proximal part of the jejunum were recovered 45 min after the end of the infusion and the mucosa was scraped off with a spatula (OEA measurement), frozen immediately in liquid nitrogen and stored at −80°C until used.
Plasma and tissue measurements
Commercially available EIA kits were used to measure the plasma level of insulin (Crystalchem, Chicago, USA) and Cholecystokinin, CCK (Phoenix Europe, Karlsruhe, Germany). Plasma glucose and triglycerides were measured using commercial kits (BioMérieux, Marcy-l'Etoile, France).
For lipid analysis, lipids were extracted by 2mL of CHCL3/MeOH (2/1,v/v) at 85°C for 1h. After addition of 2mL NaCl (2.5% w/v), the extract was vortexed and the above phase discarded. The chlorofomic phase was evaporated and dried under a nitrogen stream and the lipids were dissolved in 100 µL of CHCL3/MeOH (2/1,v/v). The lipids were separated by TLC using chloroform/methanol/1-propanol/methyl acetate/0.25% KCl (10/4/10/10/3.6, by vol.) or pyridine/chloroform/formic acid (25/15/3.5, by vol.). The N-oleoyl-phosphatidylethanolamine (NOPE) is identified by comparison of the Rf with the N-acylphosphatidylethanolamine (NAPE) used as a standard. The spots corresponding to the NOPE were scraped and subjected to a transesterification for 1 h at 80°C in the presence of 3 mL 5% sulfuric methanol containing C17:0 fatty acid as internal standard. The fatty acids were separated by GC and the lipids were quantified by comparison of their peak areas with the standard C17:0 as described by Testet et al [23].
Quantitative RT-PCR (RT-qPCR)
Quantitative RT-PCR (RT-qPCR) was used to study the expression of different target genes. Total RNA was extracted from tissue samples using TRIzol (Invitrogen, Cergy-Pontoise, France) according to the manufacturer's protocol. All samples were treated by DNase I (Invitrogen) before the reverse transcription. First strand cDNAs were prepared using one µg RNA and the Moloney Murine Leukemia Virus Reverse Transcriptase, MMLV (Fermentas, St. Rémy-Les-Chevreuse France) in the presence of oligo(dT) primers (Fermentas). The qPCR reactions were performed using the Light Cycler Fast Start DNA Master SyBR Green I kit (Roche, Meylan, France) in the presence of specific primer pairs (Table 2). Samples (in duplicate) were quantified by comparison with a standard curve obtained by dilutions of purified target cDNA fragment and mRNA level was expressed as a ratio between values obtained for the target gene versus values obtained using the housekeeping genes L19 (intestine) and GAPDH (hypothalamus).
Western Blotting
The jejunum was cut in three parts of equal size and mucosa was scraped off from the first part (proximal jejunum). Ten µg of proteins, prepared from jejunal mucosa, were separated by SDS-PAGE 10% and transferred to a PVDF membrane (Immobilon-P transfer membrane, Millipore, Saint-Quentin-en-Yvelines, France). Immunoblotting was performed using goat antibodies directed against mouse CD36 (1,000-fold dilution, R&D Systems Europe, Lille, France) or mouse monoclonal antibodies directed against mouse beta-actin (1,000-fold dilution; Sigma-Aldrich, Saint Quentin Fallavier, France). Goat anti-mouse (BioRad Laboratories, Marnes-le-Coquette, France) and donkey anti-goat IgG (Santa Cruz Biotechnology, Tebu-bio France, Le Perray en Yvelines) were used as secondary antibodies for beta-actin and CD36, respectively. Blots were revealed using the Immobilon Western Blotting Chemiluminescent HRP Substrate (Millipore SAS, Molsheim, France). Quantitation of the bands was performed using Adobe Photoshop software.
All statistical analyses were performed using one-way ANOVA, followed by post hoc testing with Fisher's protected least square difference test (PLSD), with the aid of Statview 5.0 software package (SAS Institute Inc. Cary, NC 27513). Results were expressed as mean ± SEM and differences were considered significant at P<0.05.
Binding of intestinal CD36 with the pharmacological drug SSO reproduces the lipid-mediated inhibition of food intake in refed mice
To determine the kinetic of duodenal lipid infusion on food intake, fasted mice bearing a permanent duodenal catheter, were infused with saline or 20% IL (6 µl/min for 10 min). Food intake was significantly lower 30 and 60 min after the end of luminal infusion of IL as compared to saline solution. For example, food intake dropped from 0.33±0.02g (0.96kcal) to 0.24±0.01g (0.70 kcal) in control and IL mice 30 min after infusions, respectively (Fig 2-A). This satietogen effect was transient since it was not retrieved 120 min after the end of infusion (Fig 2-A).
(A): Food intakes were measured 30 min, 1hr and 2 hr after the end of the infusion and were not cumulated. Each type of infusion was performed several times on the same animal. * P<0.05 relatively to saline infusion (for each period of time). The dotted line corresponds to the reference value at each time (mice infused with saline). The number of experiments (n = 16) corresponds to different animals. (B): The expression of the gene encoding CD36 was measured by RT-qPCR on RNA samples obtained 45 min after the end of the infusion. Results were expressed as mean ± SEM (CD36 versus L19). * P<0.05 differences between saline and IL infusions for each part of the small intestine.
Expression of CD36 encoding gene was measured by RT-qPCR in intestine serially divided in 6 segments of equal size, according to previously described procedures [24]. Consistent with previous published data [10], [11], a progressive decrease in CD36 mRNA levels was found from duodenum to ileum in control mice (Fig 2-B). In contrast, a 2-fold decrease in CD36 gene expression was found in duodenum of IL-infused mice as compared to controls (Fig 2-B). There was no difference between saline and IL infusions in the other parts of the small intestine suggesting that the amount of lipids infused in duodenum was too small to reach another part of the intestine and affect the expression of Cd36 in jejunum and ileum (Fig 2-B). No significant differences in Agouti related Protein (AgRP) and Proopiomelanocortin (POMC) gene expression between saline and IL-infused mice were found in hypothalamus removed 45 min after the end of infusions (data not shown). Thus, the decrease in food intake mediated by an acute infusion of minute quantities of IL in duodenal lumen was not due to any alteration in the expression of the genes that are directly involved in the control of food intake at the hypothalamic level.
To further explore the intestinal CD36 implication either as a AGLC transporter [19] or a putative receptor [13] in the lipid-mediated control of food intake, pharmacological manipulation of the ligand binding site of CD36 was performed using an infusion of the sulfosuccinimidyl-oleate (SSO) into the duodenal lumen prior to IL or saline treatment in wild-type and CD36-null mice. SSO is known to be a specific and irreversible ligand for CD36. By this reason, it is likely that SSO is able to bind to intestinal CD36 receptor as well as inhibit lipid binding. As expected, a decrease of food intake took place 30 min after infusion of IL in wild-type mice (Fig 3). Surprisingly, a more drastic effect was found in SSO-treated wild-type mice in which subsequent infusion of IL did not induce an additive inhibitory effect on food intake (Fig 3). Thus, the irreversible binding of SSO to intestinal CD36 was sufficient to reproduce the satietogen effect of IL infusion. To verify this interpretation, we performed the same protocol in CD36-null mice. Basal food intake measured 30 min after saline infusion was significantly lower in CD36-/- mice than in WT (Fig 3). When CD36 gene is lacking, neither SSO alone nor IL infusion alone or in association with SSO induced any change in food intake (Fig 3). Therefore, the binding of intestinal CD36 with a pharmacological ligand is sufficient to induce satiety. It is noteworthy that this effect is not secondary to lipid-mediated changes in the satiety hormone CCK since its plasma levels remained similar between the different groups (data not shown).
Food intakes were measured 30 min after the end of each type of infusion performed either on wild-type (CT, n = 15) or CD36-invalidated mice (n = 9). a: P<0.05 relatively to Pyrr/saline infusion in CT; b: P<0.05 relatively to Pyrr/IL infusion in CT.
Taken together, these data are consistent with a role of CD36 in satiety as lipid receptor able to detect the presence of dietary lipids in intestinal lumen rather than lipid transporter as previously suggested [19].
Chronic high protein diet affects intestinal CD36 gene expression, lipid-induced OEA synthesis and satiety
Was the short-term hypophagic effect of an acute lipid load maintained when the mice were adapted chronically to a satietogen diet? To address this question, we studied the effect of the initial administration of an imbalanced diet to mice, on the response to an acute lipid infusion. For this purpose, wild-type mice were subjected for several days to a high-protein diet (HPD). Indeed, it is well known that a chronic HPD is highly satiating and the small intestine is able to adapt its gene expression to its dietary environment [25]. HPD led to a 40% decrease in food intake, from day 1 to day 5. Afterwards, this satiety effect was stabilized at around 15-20% below the control levels (standard chow-fed mice) (Fig 4-A). There were no significant differences in food intake between mice fed either standard chow or HPD in CD36-null mice (Figure 4B). This unexpected data suggests that CD36 gene was required for the satiety effects of HPD. This HP diet up-regulated the intestinal CD36 gene expression (mRNA and protein levels) in proximal intestine of wild-type mice fed on HPD compared to control mice fed on standard laboratory chow (Fig 4-C & D). This effect was more drastic after 4 days of HPD, when food intake was especially low (Fig 4-A), than at days 12 and 30 (Fig 4-C). This effect seems to target specifically CD36, since no induction of the expression of genes encoding other lipid-binding proteins such as L-FABP was found (data not shown). HPD-mediated change in the intestinal CD36 gene expression was associated with a decrease in plasma triglyceride and insulin levels after 4 days of diet, while the decrease in food intake was especially pronounced (Fig 4-E and -F).
(A-B): Food intake relative to body weight during 12 days of standard or HP diets. (A) corresponds to wild-type (WT) mice and (B) to CD36-invalidated mice (C): CD36-encoding mRNA level (relative to L19 gene) in the duodenum and proximal jejunum of WT mice fed standard or HP diet. (≠ P<0.05 relative to duodenum control; * P<0.05 relative to jejunum control; n = 5 to 8). (D): Western Blot analyses of proteins prepared from proximal jejunum of WT mice on standard (Control) or HP diet for 4 and 12 days. Each lane corresponded to a different mouse. (E): Plasma triglycerides measured in control and HPD-fed WT mice. a: P<0.05 differences between control and HPD mice at each period of time; b: P<0.05 relatively to HP 4days. (F): Plasma insulin measured in control and HPD-fed WT mice. a: P<0.05 differences between control and HPD mice at each period of time; b: P<0.05 relatively to HP 4days.
To explore the impact of this HPD-mediated up-regulation of CD36 gene expression on regulation of food intake, saline or IL infusions into duodenum were performed. As expected, HPD-fed mice had a significant lower food intake levels than control group (Fig 5-A). In contrast to the standard chow-fed mice, in which IL infusion led to a decrease in food intake, no significant satietogen effect of IL was found in mice fed HPD (Figure 5-A). Moreover, the decrease in the duodenal CD36 mRNA levels found in mice fed the control chow and subjected to an IL infusion, was not significantly different in HPD fed animals (Fig 5B).
Mice were fed either standard chow or HP diet 12 days before surgery. Saline and IL infusions were performed after one-week recovery. (A): Food intakes were measured 30 min after saline and IL infusions (n = 29 for CT and 17 for HP). (B/C): Duodenal CD36 mRNA level (B) and jejunal OEA concentration (C) were measured 45 min after the end of saline and IL infusions. a: P<0.05 relatively to saline infusion in control group.
There was no significant difference in plasma levels of insulin and glucose 45 min after IL infusion and refeeding, compared to saline infusion (Table 3). As well, there was no difference in insulin level between control and HPD-fed mice but a reduction of glycaemia was observed in HPD-fed mice compared to controls (Table 3).
The lipid messenger OEA was measured in intestinal mucosa 45 min after the end of saline or IL infusions in mice fed the standard chow or HPD. Consistent with previously published data [19], OEA was increased in standard chow-fed mice after IL infusion relatively to saline (Figure 5-C) which is consistent with the IL-mediated decrease in food intake. The concentration of OEA after saline infusions was significantly higher in HPD-fed mice than in standard chow-fed mice, but no additive effect on intestinal OEA levels occurred after IL infusion in contrast with what was found in mice fed the control chow.
Acute infusion of minute amount of lipids (0.12 kCal for 10 min) in duodenum from 5h-fasted mice induced a transient reduction of food intake during refeeding. Our data show that the binding of intestinal CD36 with the pharmacogical drug SSO is sufficient to reproduce this lipid-mediated regulation. Therefore, the ligand binding of intestinal CD36 appears to be a major step in the onset of the satiety triggered by the lipid content of the diet. Along with a decrease in food intake, the lipid infusion induced a decline in CD36 gene expression only at the site of IL infusion (i.e. duodenum). Such a reduction of CD36 mRNA levels has previously been observed after 1hr of intraduodenal infusion of oleate in rats [11]. This long-lasting infusion induced a diminished CD36 expression not only in duodenum but also in the jejunum part of the intestine. Thus, the lipid-induced down-regulation of the CD36 mRNA extended to the two proximal parts of the small intestine after a 1hr-infusion was limited to the duodenum after a shorter infusion. Such differences observed on cd36 gene regulation could likely be related to the duration of the infusion and the amount of fat available to bind the protein CD36 localized at the site of infusion. In line with a potential role as a lipid receptor, which has been proposed for intestinal CD36 [13], [26], we might therefore draw a parallel between these effects and the well-known down-regulation phenomenon induced by some agonists on their specific receptors [27], [28] that could reflect an activation of the protein CD36. Contrary to this rapid decrease in CD36 gene expression after an acute infusion of lipid, chronic feeding with a high fat diet induced an induction of CD36 mRNA levels [10], [25] suggesting, in this case, an adaptation to the diet to favour lipid absorption.
A satiating effect of dietary fat has previously been described, mainly by using rat infused with a higher amount of fat during longer periods of time [18], [29]. Recently, Schwartz et al [19], using short infusions of a low amount of lipids (0.2 kcal Intralipid during 10 min), have demonstrated a reduction of the consumption of a liquid diet, as measured 30 min after the end of the infusion in wild-type mice, but not in cd36-invalidated mice. Our results are in good agreement with these findings. As the transgenic model previously used by Schwartz et al [19] is not a tissue-specific invalidation, we instead preferred to use the sulfosuccinimidyl-oleate (SSO) to limit the inhibition of the CD36 protein to the small intestine. This compound was first described as an inhibitor of LCFA uptake in adipocytes [30]. It specifically binds to the CD36 protein and has been mostly used for in vitro studies in several cell models [31], [32] where it alters LCFA transport by CD36 but does not affect the metabolism of these fatty acids [32]. When SSO was infused into the duodenum 5 min prior the saline or IL perfusions, there was no modification of food intake by IL, demonstrating the involvement of the intestinal CD36 in the feeding reduction induced by lipid infusion. However, when SSO was infused prior the saline infusion, a decrease in food intake compared to mice infused Pyrr/saline, was already observed that seems totally dependent on the presence of CD36, as this effect was completely absent in cd36-knockout mice. Such a CD36-dependent decrease in food intake induced by SSO alone might depend on its capacity to elicit cell signalling further to binding CD36, in addition to its known role as a competitive inhibitor of fatty acid binding. Such a lipid binding effect has been recently described by Tran et al [13]. Therefore, the reduction in food intake after acute fat load and refeeding was clearly in direct relation with the presence of a functional CD36 in the small intestine.
What is the contribution of this CD36-mediated satietogen effect in a long term satiety-inducing diet? Some studies have demonstrated that the duodenal infusion of lipids in rats inhibits food intake by decreasing meal frequency, but not the meal size [33]. As well, when rats where adapted to a high-protein diet, they reduce the number of meals when compared to rats fed a standard diet [34]. This type of imbalanced diet has been currently used to help humans to lose weight [35] and mechanistic studies have been performed in animal models [36], [37]. Unexpectedly, chronic HP diet, like chronic HF diet [25], induced an increase in CD36 mRNA and protein levels in duodenum and proximal jejunum. These results suggest that the potential capacity to absorb dietary LCFA was increased by this HPD, as well as the capacity of the intestine to signal for a decrease in food intake. Prior to the surgery, all the mice fed on a HPD responded by a decrease in the daily food intake. A reduction in food intake was also observed in HPD mice after saline infusion and refeeding, as compared to infused standard-fed mice, but there was no further decrease after IL infusion. The absence of down-regulation of CD36 mRNA level by IL, in the HPD group might be related to a defective activation of CD36 by lipid infusion and could explain the absence of an additional decrease in food intake.
It has been postulated that OEA acts as a messenger to control the anorexigenic effect of lipids [19]. When directly administered to free-feeding rats, OEA inhibits food intake by delaying meal initiation [38], [39]. In food-deprived animals, OEA delays feeding onset but also reduces meal size [38]. This lipid messenger is produced by proximal small intestine [40] and is an endogenous ligand for peroxisome proliferator-activated receptor-α, PPAR-α [41]. The level of its synthesis is decreased by fasting and increased after refeeding [39]. Moreover, OEA is capable of increasing CD36 mRNA expression in intestinal mucosa [42]. Schwartz et al [19] have previously shown that small-intestine OEA production by mice increases by 30 min after a refeeding which follows a 6h-fast, when compared to the level measured just before refeeding. Herein, we showed that OEA level increased 45 min after IL (versus saline) perfusion of mice, as previously demonstrated for IL infused rats [19]. Moreover, when measured 45min after the end of the saline infusion that corresponds to the beginning of refeeding, the jejunal OEA level was much higher in HP group than in control group. We can postulate that this excessive OEA production in response to fasting and refeeding might partly explain the sustained decrease in food intake observed in chronic feeding with HP diet. This higher amount of OEA produced by proximal jejunum in HPD-fed mice versus controls (standard chow-fed mice) is in good agreement with the higher expression of CD36 in the proximal small intestine of HPD-fed mice [42]. Moreover, there was no OEA overproduction in response to IL infusion in the HPD group. The reason is probably the excessive jejunal OEA production already induced by refeeding in HPD-fed mice compared to control mice, which might be sub-maximal, making it independent of fat infusion. Indeed, Schwartz et al [19] have demonstrated that OEA is increased after refeeding in standard chow-fed mice, but results described herein indicate that this increase was more pronounced in HPD-fed mice. This effect is CD36-dependent as after cd36 deletion it was lost [19]. Moreover, the use of CD36 null mice indicated that the hypophagia effect of the high-protein diet was, at least partly, dependent on the presence of CD36. Indeed, other mechanisms have been described to explain the reduction of food intake obtained after HPD [36], [37], [43]. Protein feeding induces a redistribution of endogenous glucose production and, in particular, it increases the amount of glucose released by the small intestine that in turn initiates satiating signals to the brain [36].
We postulate that OEA production may be induced by at least two different mechanisms. An acute intestinal lipid load generates OEA synthesis which requires a functional CD36 protein [19]. In the case of an adaptation to a chronic high-protein diet, wild-type mice exhibit an exaggerated response to refeeding, leading to OEA overproduction. In this case, an acute lipid load will not produce any further increase in OEA production as well as no additional decrease in food intake.
In conclusion, data presented herein, demonstrate that CD36 present in the small intestine is an active player in the lipid-mediated induction of satiety. The experiments using SSO in WT and CD36-null mice, suggest that lipid binding to intestinal CD36 is sufficient to produce a satiety effect. These data suggest that CD36 might control the OEA production/mobilization via an activation of cell signalling [13] which in turn might modulate the activities of NAPE and/or FAAH [19]. Moreover, CD36 could participate in the satiety effect induced by HPD. The small intestine has a high capacity to adapt to different types of diet. This has been demonstrated for lipid-enriched diet, with the acquisition of an increased capacity to absorb fat in response to the enrichment in dietary fat. Herein, we show that intestine can also adapt to a protein-enriched diet partly by CD36-dependent mechanism and participate to the modulation of food intake by several mechanisms including an increase in OEA production and CD36 gene expression. Furthermore, intestine of mice adapted to HP diet have a diminished capacity to modulate their food intake in response to dietary lipids present in the meal. Overall, our study brings new data in favour of the hypotheses advanced by some authors of a specific role of intestinal CD36 as a lipid receptor.
We thank Angèle Chamousset, Chystelle Segura and Mickael Gay for animal care and Jean-Michel Vicat for the housing and care of the CD36-invalidated mice. The lipid analyses were performed using the Metabolomic facilities of the Bordeaux Genomic Center.
Author Contributions
Conceived and designed the experiments: DN HP IN MB PB GM. Performed the experiments: DN AD MV DO. Analyzed the data: DN RL MB. Contributed reagents/materials/analysis tools: DN AD MV DO. Wrote the paper: DN RL HP IN MB GM.
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For Clinicians
In order for the stem cells in cord blood to be able to save it life it my first be collected from the umbilical cord. Then, after testing and storing, the stem cells must then be transplanted to a recipient in need.
Here health care providers will find the information needed to both collect and transplant stem cells via cord blood units.
- Collection guidelines (SOPs)
- Collection instructions
- Collection forms
- Transplant center requirements
- Transplant center forms
You can browse our bibliography of professional publications, or if you have any questions, concerns or need to return completed forms, please contact us.


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Kirkmchael Village Renaissance - KVR - was formed as a not for profit limited company in 2010 It acts for the benefit of the community and supports events and development projects in the village. The Community Shop and Café is the trading arm of KVR.
Exciting events to look out for
McCosh Hall handback party - more details when the school moves to its new home.
Mid year music festival.
Firework night Wednesday November 5th at 7.30 at the Play park
Do you want to make the village even better?
Create a better play park? Restore the Community Garden? Organise a music festival? Help with the website? Join in a Reminiscence project with the school children? Organise a car boot sale?
Over £35,000 raised through grants and fundraising for the community since 2010
Latest meeting minutes (opens in new window):
Contact your Board members
Caroline Lorimer, Chair: 01655 750350, email@example.com Haston, SecretaryNorman Wilson, Treasurer
Patrick Lorimer, James Hall, Eleanor Scobie, Frank Potter, Gordon Pettigrew, Campbell Smith


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Roland Gautreau
Roland "Goat" Gautreau, 65, of Lakeville, passed away peacefully on Friday, January 12, 2018 with his beloved wife Rose at his side. Born October 25, 1952 in Moncton, he was the son of the late Edward and Augustine (Melanson) Gautreau. Roland was employed with White Cab before his retirement. He enjoyed playing his electric guitar and spending time with his family and friends. Besides his wife, Rose née Fougère, Roland will be sadly missed by a brother, Leonard (Carole) of Riverview; many nieces and nephews. He is also predeceased by a brother, Robert "Bobby". The funeral service will be celebrated at the Frenette Funeral and Cremation Centre, 396 Main Street, Shediac (532-3297) on Tuesday January 16 at 11 am. Interment will be in the Frenette Columbarium, Shediac. In memory of Roland, a contribution to a charity of the donor's choice would be appreciated. Words of comfort and donations made be made at www.frenettefuneralhome.com


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Our intention is to acquire and make available ALL picture books featuring indigenous people and people of color published in the U.S. since 2002, including reprints. Inclusion of a title in the collection DOES NOT EQUAL recommendation. See our related readings page for suggested links for evaluating books.
Filter Results
Cross Group 25
Activism 2
Fiction 13
Non-Fiction 12
The rabbit-hole golf course
"In the big old ute, on the long red road, in the desert of my home, we all set off for the rabbit-hole golf course. It's the best place around here to find rabbits. We sit by the holes, we dig, we wait ... Thump tick, thump tick, thump tick Where are all the rabbits? A warm and funny Australian story"--Back cover
Say yes
"Once there were two little girls who were best friends. They did everything together. As they got older they weren't allowed to do the same things anymore. Because they looked different. Because of the law. This is a story about the landmark 1967 Referendum, the two women who came together to change the law...and how the Australian people said YES"--Back cover
Mr. Crum's potato predicament
When a fussy patron sends his order of potatoes back twice, chef George Crum decides to have some fun, based on the true story of the potato chip
Crossing Bok Chitto
In the 1800s, a Choctaw girl becomes friends with a slave boy from a plantation across the great river, and when she learns that his family is in trouble, she helps them cross to freedom
The people shall continue
"The People Shall Continue was originally published in 1977. It is a story of Indigenous peoples of the Americas, specifically in the U.S., as they endeavor to live on lands they have known to be their traditional homelands from time immemorial. Even though the prairies, mountains, valleys, deserts, river bottomlands, forests, coastal regions, swamps and other wetlands across the nation are not as vast as they used to be, all of the land is still considered to be the homeland of the people"--Foreword
The great expedition of Lewis and Clark
An account, told in the words of one participant, of the difficulties and wonders that were part of the Lewis and Clark expedition to explore the land obtained as part of the Louisiana Purchase
Jemmy Button
Provides a fictionalized account of Jemmy Button, a native boy from Tierra del Fuego who was brought to London to be educated and then returned home to his island
The red sash
A young Native American boy waits for his father's return while he discovers what life is like at a busy fur trading post, until he must make a decision on his own as an unexpected storm appears on the lake
When I was eight
This book chronicles the unbreakable spirit of an Inuit girl while attending an Arctic residential school
Sitting Bull
Sitting Bull (c. 1831-1890) was one of the greatest Lakota /Sioux warriors and chiefs who ever lived. From Sitting Bull's childhood -- killing his first buffalo at age 10 -- to being named war chief to leading his people against the U.S. Army, this book brings the story of the great chief to light. Sitting Bull was instrumental in the war against the invasive wasichus (white men) and was at the forefront of the combat, including the Battles of Killdeer Mountain and the Little Bighorn. He and Crazy Horse were the last Lakota/Sioux to surrender their people to the U.S. government and resort to living on a reservation


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Japanese Language Division Information Session is scheduled for February 3, 2018. If you are seeking admissions information, internal transfer information from other JLS divisions, or any other information, please attend the Information Session described below. If you are seeking advice for internal transfer, please ask your child's homeroom teacher or contact the school office. If you are considering an internal transfer of your child to the Japanese Language Division, please notify us by February 3, 2018.
(1)Information Session
When: February 3, 2018, 11:10 – 12:00 (3rd Period)
Where: 2nd Floor Lecture Hall
Overview and Curriculum
(2)Open House
When: February 3, 2018, 9:15 – 10:50
Where: J1, J2, J3, J4 & J5 (C101, C103, C105, C201 & C203)
*Pre-registration is not required.
*If your child will attend the open house, please notify your child's homeroom teacher by January 27, 2018.
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Name of business: Petz Paradise LLC
Owner: Valerie Bailey
Address: P.O. Box 9, Clear Spring, MD 21722
Opening date: December 2007
Products and services: Pet sitting, walking and taxi, in-home baths; grooming and homeopathic products are coming soon
Market area: Hagerstown, Clear Spring and surrounding areas
How did you get into your business, or what motivated you to start it? "I have loved animals since I was little, and I enjoy working with them. Volunteering with animal rescues is something I have enjoyed.
"Whatever the assignment - scrubbing cages, feeding and watering animals or socializing them - gives me great satisfaction. Enhancing animals' lives is my goal."


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My First Experience with Christian Hip Hop feat. Alex Faith
As part of Sketch the Journalist's "CHH 101" series of posts for WadeORadio.com about the history of the gospel rap movement, he'll be sharing video interviews he's conducted with some of the genre's top names about their first experience with Christian hip hop.
In this outing, Alex Faith talks about the way he was introduced to hip hop made by Christians.
How would you react if you ended up working beside an artist whose music you had once illegally downloaded?


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Serve up ham for the holidays with Cobblestreet MKT. Carving Ham. A bourbon cider glaze makes this traditional roast a delicious festive feature.Read More »
Tag Archives: Holidays
Focus on Fusion this Valentine's Day
It's the Ideal Occasion for Pairing Up the Unexpected Everyone knows an odd couple: Polar opposites who make the perfect pair. When creating your Valentine's Day menu, take an unconventional approach to matchmaking and pair up textures, flavors, and ingredients from different culinary traditions. Your goal should be a menu that's full of surprising twists, but not too "out there." ...Read More »
Top 5 Ways to Extend Valentine's Day Sales
Valentine's Day is a bittersweet occasion for restaurant owners: On one hand, the holiday sends sales soaring for a day. But during the rest of February, it's midwinter business as usual, which can be on the slower side. Don't settle into a slump, instead keep the momentum going with the following these recipes and tips for a full month of ...Read More »
Throw a Mardi Gras Party
Mardi Gras, which means "Fat Tuesday" in French is a festival of indulgence before the Lenten Season starts. Originating in New Orleans, Mardi Gras is known for its signature colors which are purple, green, and gold -colors chosen by the King of Carnival in 1872. Throw a Great Party As the owner of your restaurant you automatically become your events ...Read More »
The Flavor Gap: Your Independent Advantage
Singles don't feel so lucky on Valentine's Day, but independent restaurants have an advantage over the chains when it comes to capitalizing on the current "multi-ethnic flavor trend." According to Baum & Whiteman International Food and Restaurant Consultants, the chains can't deliver the complex global flavor profiles the customers are craving today, because by nature, chains are "compelled to serve ...Read More »
Romance Customers with an Aphrodisiac Inspired Menu
Customers always enjoy the "story" behind their food: what local farm raised their meat, how long your restaurant simmered its house-made stew, or where your chef traveled for an inspired dish. During the month of love, offer a few items with ingredients believed to be aphrodisiacs in certain cultures on your menu. Here are a few menu ideas from the ...Read More »
5 Ways the Spirit of Giving Can Lift Restaurant Revenues
The holidays are characterized by a spirit of generosity and there are numerous ways that restaurants can tap into that mood to boost profits and elevate customers' spirits. Below is your guide to some of the easiest gift-and-giving-related tactics to improve your profits and visibility this holiday season. 1. Gift Cards Increase Profits Consider gift cards to be your restaurant's ...Read More »


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Central Administration
Timothy T. Eagen, Ed.D.
Superintendent of Schools
Ralph J. Cartisano, Ed.D.
Assistant Superintendent for Curriculum and Instruction
John T. Craig
Assistant Superintendent for Personnel and Administrative Services
Danielle Colby-Rooney, Ed.D.
Administrator for Pupil Personnel Services
Shannon L. Meehan, CPA
Assistant Superintendent for Business
Board of Education
Pam DeFord, President
Diane Nally, Vice President
Joe Bianco, Trustee
Kevin Johnston, Trustee
Dan Tew, Trustee
High School Administration
Lino E. Bracco, Principal
Andrew Akapnitis, Assistant Principal
Jeff Harris, Assistant Principal
Maureen Raynor, Assistant Principal
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