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You recently interviewed a group of people who are very concerned about air pollution levels in Shoreham town centre, due to increased traffic which, inevitably, leads to poor health conditions.
I would like to add my fears that the proposed housing development under the Adur Local Plan will undoubtedly create more traffic and pollution and Adur District Council should give this matter serious consideration before such decisions are taken.
I have lived in Shoreham for 12 years and cannot believe how much extra traffic is being generated. I appreciate more homes need to be built but not to the detriment of people's health.
I strongly feel this matter should be given as much coverage as possible to raise awareness of the situation.
Pat Connolly
Harbour Way
Shoreham Beach
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Friends came in some many shades of colors that add so much to our lives. I have some dear friends, like all of you, that means so much to me. Then there are those that no matter what will forever be a friend. And then there are many of you that have married your best friend, and that is even better. To develop a life long relationship is an balancing act. And only when we can fully accept the other we will form a true and forever friendship.
I made this card today more leaning towards an anniversary / wedding card. I think it is a perfect card for that occasion, but I can see it myself giving it to a best friend. The sketch is from the PPA 314 and it was a fantastic inspiration for me. I used the new Falling Flowers for the image, which was stamped twice in opposite sides. The center panel covered part of the stamp rather well and I made sure to do my best to center the image. The sentiment is from the First Sight stamp set, and it was cut using the Lots of Labels framelits. I used a strip of paper to stamp, and then centered it on the framelit obtaining a rounded edge. I add a bit of the Tip Top Taupe ribbon because it is just too beautiful and kept to the color scheme of the card. A striking card with very little supplies and that can be put together in little time.
Thank you for your visit today. Hope to see you again soon. Be well
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subset: commoncrawl


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Alton teachers reject contract offer
District, teachers to meet with federal mediator in hopes of a solution
GODFREY — Teachers in the Alton School District voted overwhelmingly Thursday to reject the district's latest contract offer.
According to representatives from the Alton Education Association (AEA), the proposed contract was rejected by 90 percent of voting members in attendance. AEA president Brenda Powers said it was "one of the worst offers" from the district yet.
"We have done everything we could to help the district," Powers said, citing an agreement between the district and AEA three years ago in which teachers sacrificed their step raises for the good of the district.
A copy of the proposed contract obtained by the Telegraph revealed the teachers would again be going without step raises, which are given to teachers for longevity and continued education. Under the proposed contract, the district would grant a 1.4 percent increase in salary across the board, however. The offer also stated there would be no changes in insurance benefits or in the current retirement agreement.
With the rejection of the contract, the district and AEA will next meet with a federal mediator for a second time, according to Illinois Education Association (IEA) UniServ Director Ric Stephenson. Stephenson added that the union didn't recommend that teachers vote either way on the district's contract proposal.
"We wanted our members to choose for themselves," he said.
The contract rejection is another blow to the Alton School District, which is suffering tremendous financial woes at present. The district Tuesday announced its plan to restructure schools so that attendance will be based on grade levels, not location as it is currently. The change will be made by the 2016-2017 school year.
Powers said the move will cut the jobs of several teachers and violates a promise the district made to the community 10 years ago.
"This is the district's way of reducing staff to try and reduce spending," Powers said of the announcement. "It goes against their earlier promises of keeping neighborhood schools."
Alton School District Superintendent Kenneth Spells said the attendance centers were among his "worst-case scenario" plans. Alton School District Financial Director Christopher Norman said the district is working at a $2 million deficit due to declining property tax rates and proration of state funding.
Reporter Cory Davenport can be reached at (618) 208-6447 or on Twitter @CoryTelegraphs.


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Spring, spring…why I fucking love you. If you can't tell spring is my favorite season. The temperature is perfect—there are warm days, rainy days, stormy days, beautiful days—the flowers bloom, the birds chirp, and days get longer, I could go on and on. The most amazing thing about this season is how beautiful nature becomes. The hibernating animals come out with their babies, plants and trees bloom bright, vibrant colors, the sun shines with a purpose, and the air feels so much more crisp & fresh.
I sound like a corny sap, but I love spring. The vibe and appreciation I feel from the visible new life uplifts my mood and gives me (literally) life.
I've always loved spring, maybe because I have a huge appreciation for nature, but also because it means my birthday is coming up! March 14th y'all, don't forget. Funny, I was supposed to be born on the first day of spring, March 21, but I was born a little early.
Anyways–an update about my life: I am doing well at Gap, my mentor is trying to help me hopefully get full-time! That is such great news, knowing that I have someone who believes in me and wants to see me succeed. Yesterday we went to the Livermore outlets to do a store visit. Here, we went to an actual Gap Factory store where we see all of our hard work actually transcribed to real life. It's amazing to see the projects I've worked on become actual in-store signage. After, we walked around the mall, getting inspiration from other stores & promotion. After that I went to San Ramon to look after my baby, Carl. He's been sick all week and finally yesterday, he actually began eating like normal. He hated when I took all these pictures with him, but wtv I'm obsessed with pink cherry blossoms (my favorite part of the season, so sad it only lasts a few weeks).
Spring is also a fav because I'm obsessed with spring fashion. Lighter layers, pastel colors, & FLORAL PRINTS! I've been super obsessed with baby pink & floral prints lately. My wardrobe mostly consists of black, but when it's spring I love to incorporate a bright baby pink bomber or a new bold floral jacket over my all black outfit. I've also been really diggin Asian silhouettes and prints. I mean, I am Asian, why not take full advantage of my heritage & express the love of my culture through my clothing. That's what fashion is about, expressing who you really are through your clothes. Take a look of my current obsessions and outfit choices.


text: Vogue Flowers has been voted the Best of Richmond for 20 consecutive years! We are The Flower Experts! If you can't find a particular bloom at Vogue, you probably can't find it anywhere in Richmond. Whatever customers envision whether it is flowers for an occasion, DIY or for their wedding and big events Vogue works to make your vision a reality. Some of the flowers are locally grown on our own farm! Vogue Flowers are The Flower Experts because Experience Matters!


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The sky is gray and close… Looks and smells like snow!
Tag Archives: snowstorm
Looks Like Snow…
Filed under Project 365
Blizzard of 2009
The official reading for Bensalem: 11 inches.
Dad to the rescue! He brought his snowblower over (even though it was all done!) and made it better. Then he plowed the neighbor's driveway and two houses' sidewalks. (Unlike the THREE people I saw with snowblowers this morning *who actually live here* who did only their own house. Nice!)
Sidewalk after the snowblower. Much nicer!
The Saturday before Christmas is when Santa comes around the neighborhood on the local volunteer fire truck. I heard one siren wail yesterday afternoon but never saw anything. (It takes hours for them to go around. And, it could've been for an emergency, but we don't ever hear sirens with that…) This afternoon I head the siren, and it was close, so I threw on my boots and they were right out front! I don't know why it's so thrilling.
And there it is, The Blizzard of 2009!
Filed under Project 365
Why People Buy Bread, Eggs and Milk before a Snowstorm
Just kidding. 😀 Though that is the big joke, that people run out to buy the ingredients to make French Toast just because they're going to be snowed in. Are milk, bread and eggs really the most basic staples we need? Who knows.
By the way, these are Bird's Nests, not French Toast, and they were quite yummy – and a little more worthy of reading Julie and Julia while eating. 😉 Although I am sure they do not appear in Mastering the Art of French Cooking.
Filed under Memories, Pop Culture, Project 365


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As we move into the winter months, the Winkle Club looks back on a successful Autumn period and Festive Season.
It was in early September that we commemorated the 60th anniversary of Sir Winston Churchill becoming a member by watching Pathe News coverage from 1955 and by listening to Glynn Gifford-Boyd, from the local family of photographers, who was present on Winkle Island when Sir Winston received his gold winkle.
Later in the month, the Island was transformed into 'Fantasy Island ' for a day ( in conjunction with the Seafood & Wine Festival ) complete with music, dancing and side-shows etc.. Close on £1,000 was raised as a result.
October began with a Social in our H.Q., The Fishermen's Institute,and we were very grateful to the ever-popular 'Loose Ends' for providing the entertainment. Again a very useful sum was raised mainly from the Raffle takings, with all prizes donated by our generous membership.
We have now formed a small 'Fundraising & Promotions sub-committee' which has come up with many new ideas to help us raise money for local good causes and it is proving to be very successful.
On Remembrance Sunday in November the Winkle Club laid wreaths at both the Town's Act of Remembrance in Alexandra Park and at Holy Trinity Church, Claremont.
Throughout the Autumn we have continued to give talks to various Clubs and organisations about the Winkle Club's history and its hopes for the future.
December is always a busy month for our club and December 2015 seemed even busier than usual. On Saturday 12th we presented the Hastings Motor Boat & Yacht Club with a Certificate of Appreciation for their kind donation of £600. This was part of the money raised during their Golden Anniversary Year and we are most grateful to them.
On December 16th we attended the Christmas Evening at The Plough in Westfield where all the Raffle takings were donated to the Winkle Club.
Saturday December 19th turned out to be one of our busiest and yet most significant days of our entire year. We joined forces with the High Street Traders to help with the Old Town's Children's Christmas Party complete with procession from Winkle Island to the venue for the Fish & Chips plus entertainment.
In the evening, we held ,for the first time, a Remembrance Procession and commemorative event recalling those who served in the Lifeboat and Coastguard Services or who were Fishhermen or Winkle Club members. A poignant few words were said before the lantern-lit procession, lead by a lone piper made its way down All Saints Street as the church bell tolled.
On Winkle Island where wreaths were laid, Trees of Messages were in place as a tribute to seafaring loved-ones who are no longer with us. The Winkle Club intends to make this 'Christmas Time to Remember' an annual event.
The evening of the 19th concluded with a gathering in the Fishermen's Club and a Grand Raffle Draw. A BIG THANK YOU to all who helped throughout the day.
On Sunday December 20th, we delivered 50 Christmas parcels to deserving folk all nominated by committee members and/or local churches. Again thank you to all who helped with deliveries etc..
On Christmas Day, we kept up the long held tradition of singing carols for the residents of Old Hastings House and this time we were able to give them all a Christmas Card and a bag of sweeties which was rather nice.
Bringing us right up=to=date, we have just held another highly successful Annual Supper at the East Hastings Angling Association with top entertainer, Adger Brown, a wonderful meal,auction and Raffle. The Supper Evening raised around £2,500 towards Winkle Club good causes.
Now we look forward to Spring, our AGM and another busy, fun-packed winkle year ahead.
The Hastings Winkle Club wishes all members, supporters and Observer readers A Very Happy, Peaceful and Healthy New Year. Winkle Up!
Don't miss out on all the latest breaking news where you live. Here are four ways you can be sure you'll be amongst the first to know what's going on. 1) Make our website your homepage at www.hastingsobserver.co.uk/ 2) Like our Facebook page at www.facebook.com/hastingsobserver 3) Follow us on Twitter @HastingsObs 4) Register with us by clicking on 'sign in' (top right corner). You can then receive our daily newsletter AND add your point of view to stories that you read here. And do share with your family and friends - so they don't miss out!
The Hastings Observer - always the first with your local news. Be part of it.


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Lindsay Lohan Poisoned!
Alex Jones explains why Lindsay Lohan, and along extension billions of Americans is beingness poisoned on methamphetamine style does drugs alike methylphenidate that cause brain shrinking, heart troubles and a myriad of additional carks. Alex as well highlightings however selective-serotonin reuptake inhibitor prozac does drugs is turning people into psychopaths and leading to a massive increase in suicides and additional reckless behaviour.
Young young woman* and even babes is at once coming in puberty as a result of milk laced on hormones, Bisphenol A and a toxic cocktail by additional absorbed substances.
Alex as well highlightings the deadly threat by sodium fluoride and how it causes intelligence quotient decrease, bone up Cancer the Crab, and however vaccinums is as well adding to an massive and sustained chemic aggress about free human beings because the globalists' eugenics agenda goes into high appurtenances.
The wellness by our country are in grave condition. We poisonous substance ourselves with the water and food contaminated by man-made toxins. Then we poison our bears in mind and humans on untold number by prescription medicine*. At once five by six fresh does drugs about the market don't even treat the conditions for which they are prescribed.
We must wake up and take action. Our bodies and environments must be clean so human race can continue it's global awakening to the New World Order. Read the ever expanding list of SSRI stories http://www.ssristories.com/ and do an image search for "DU babies" and see photos I could not bring myself to show you in this video


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Friday, 15 October 2010
Google translate now translating Latin
Google Translate is an excellent little tool for converting between languages. Until now though it has not had that mainstay of the early scientific period, latin. While being only an alpha version and therefore not being completely accurate, it still serves to make it much easier to read and understand the gist of old documents like one of Carl Johan Schoenherr's landmark monographs on weevils, Curculionidum dispositio methodica, cum generum characteribus, descriptionibus atque observationibus variis, seu Prodromus ad Synonymiae, insectorum partem IV.
Posted by Samuel Brown at 11:21
Labels: Science
1 comment:
- Kirsty said...
Thanks Sam, that's useful to know, as I have on occasion wanted to translate from latin (herbarium specimens I think it was).
- 15 October 2010 at 12:25
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Religion & Spirituality:Christianity
The Immigrant-Friendly Church, Part 1
Today, you're going to hear about a truly unique church that has stepped up it's game to meet some truly unique needs in the community. And it's based right here in the heart of the U.S.
It's a church that has been an anchor for many in the immigrant community, in its multi-ethnic urban environment.
Host Bram Floria engages our two special guests in a lively, informative program today.
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|Undead Bard - lots of them!
The title for the Shakespeare show was long in the debating. It was going to be The Shakespeare Delusion, then Shakespeare Undead, then - oh, there was a long discussion in a coffee shop with Chris,* let's cut to the chase - Undead Bard.
Now, and this is a simple bit of wisdom I pass onto all creators of books/films/theatre/etc., the first thing to do after deciding a title is to GOOGLE IT! Because however good your title is, if someone got there first, you're stuffed. So, I Googled it - and even Binged it (other search engines are available) - and nothing major came up. Most other options have been used - Shakespeare Undead, for example, is the subtitle of a genre mashup series of books I think.
But, now the show is nearly upon us and it behooves me to check how well the title is searching - if someone types the title in, does a relevant tickets available here type page appear. And we're doing alright. We do appear a couple of times on the first page - so anyone looking for the show, but forgetful of the details, will find it.
But they'll also find a lot of beastlords. Which is nice.
The first thing that comes up is a role playing site Ever Quest and a thread featuring an undead Bard. The search engine jumped on the phrase:
"An Undead Bard says 'Give me the Mystical Lute Body you have."
Which I'm now determined to get into the show. I mean, who wouldn't want a Mystical Lute Body? I want a Mystical Lute Body. I want one NOW!
Similar finds on the same thread include: "Kill the An Undead Bard, upon death he spawns Trakanon, kill Trakanon ..." Which sounds like an analogue for a night out clubbing.
Similar finds on the same thread include: "Kill the An Undead Bard, upon death he spawns Trakanon, kill Trakanon ..." Which sounds like an analogue for a night out clubbing.
So, bring on the hordes of the undead - maybe we'll get some crossover.
* or maybe it wasn't long, maybe I'm making that up - actually I think we hit on our answer in ten minutes... and I think it was Chris, my publicist, who came up with it. It was ages ago, I can't remember these things like I used to.
Milk Bottle Productions Presents...
By multi award-winning writer and performer Robert Crighton
Shakespeare is dead, but he just won't stop talking. His words are immortal, but they keep changing. Award-winning writer and performer Robert Crighton explores the current boom in Shakespeare and how everything is now devoted to his holy name. Undead Bard is an unholy smorgasbord of play, comedy, and music, pulling apart Shakespeare, Bardolatry, and the modern world, for your pleasure.
More details about the show and online podcasts on the Undead Bard at www.milkbottleproductions.co.uk
Performing at Theatre N16
Sunday 2nd October to Thursday 13th October at 7.30pm
Matinee Saturday 8th October at 3pm
Tickets £12 / 10 concessions – https://www.ticketsource.co.uk/event/145903Theatre N16, The Bedford, 77
All this work is supported by Patreon - without the support of my patrons I could not do shows like this. To support more work like this go to www.patreon.com/robertcrighton


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by the end of college, megan realized she had fallen into an unhealthy rut: less-than-stellar eating habits and not enough physical activity. even as a college athlete, her 45-pound weight-gain and resulting hypertension and pre-diabetes diagnosis at just 23 years old served as the wake-up call she needed to get serious about regaining her health. after discovering fitbook during one fateful afternoon of insta-stalking, her weight-loss journey was jumpstarted and her life forever changed.
now, one fitbook and a fitbook lite later, she's down 60 pounds + countless inches, she said buh-bye to hypertension and pre-diabetes, and she continues to incorporate fitbook into her clean-eating and weight-lifting routine. it helps her stay on track and keeps her commitment to herself and her health strong. but don't take our word for it. here's megan's story in her own words:
I have lost close to 60 pounds and I am not diabetic; my hypertension is gone and my blood pressure is perfect.
lesson to learn from megan's triumph? this isn't just about what you see in the mirror. for her, the weight loss was a bonus; second only to her health and happiness. thank you megan for sharing your story + inspiring others to live life fit.
[read more about megan journaling her way to an 80lb weight loss – as featured on WomenHealth.com]
inspired by megan? or questions for our fab fitbooker?
let her know in the comments below! wanna be featured?
share your story, you #fabfitbooker, you.
You are such an inspiration! At just 37 years old I am a diabetic with high blood pressure and high cholesterol. I think this is the first time that I've been able to admit that without feeling ashamed. Maybe I didn't feel that this time because I know I'm finally ready to do the work. I'm getting married October 20th and I want my new, fit body to stun everyone. I got my fitbook and my meal prep containers today and my fitjournal should be here later this week. I'm ready to rock this!!!!
I ran across your instagram and saw you post about the fitbook, and I went on amazon and bought one after I saw your instagram and weight loss journey. So thank you for motivating me and helping me find this amazing tool. I'm on day 3 and LOVING it. :)
you are inspiring and I'm ordering my book today! i have all the thing you mention pre diabetic high bp like way high and i want to be around for my 4yo and be able to play with her and ride amusement park rides with her.
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Interzoo 2018
We are exhibiting our Henry Wag range at Interzoo 2018. You will find us in Hall 9 Stand 208c, part of the British pavilion.… Read
16 Apr 2018
PATS Sandown
Visit us at the Pet and Aquatics Show on stand G4 to discover our newest and most popular products. Drop by and meet the team. Beth Grant and Guy will be ready to run you through the Henry Wag experience!… Read
26 Jan 2018
Taking your pet abroad? How to get a pet passport
Like many others we are looking forward to our family summer holiday. This year it will be a little different. We're off to France for a week and for the first time we are taking our dog, Lola with us. Well, she is a part of the family. She… Read
2 Aug 2017
Crash helmets for dogs?
Are sticks dangerous for dogs? The answer is yes, according to the press this week. http://www.bbc.co.uk/news/magazine-35361664 It would appear that the number of dogs being treated by vets for stick induced injuries is on the inc… Read
21 Jan 2016
New Colour Furbuster Pet Grooming Equipment
Take a look at the fabulous Pet Grooming Equipment from Furbuster. Manufactured in the US by Petmate this great range of pet grooming equipment is now available in Teal. Click here to have a look at the full range on our website. Images of t… Read
2 Sep 2015
Hugs Chilly Mat – Cooling Mat for Dogs
Great News, we have just had a delivery of Large Chilly Mats, the fantastic cooling mat for dogs. This gel filled cooling pad requires no freezing or electrical supply, simply take it out of the box and the Chilly Mat by Hugs will keep your p… Read
18 Aug 2015
Dog Runs, Puppy Pens and Dog Kennels
Looking for a great run, pen or kennel for your dog? Look no further than our great range of indoor and outdoor dog runs, pens and kennels for your dog. ReadOur outdoor runs are manufactured by Petsafe and are great chain link dog run…
21 May 2015
Great New Products for 2015
We have some fabulous new products available for sale right now. These include dog toys, non spill bowls, Slow Feed Bowls and Self Cooling Mats. Check out out new products on line or give us a call and we will be delighted to talk you through… Read
19 Feb 2015
Interactive Cat Toys from Hugs Pet Products
Take a look at our new interactive cat toys from Hugs Pet Products. The Whack a Mouse is simple but brilliant. Essentially a box with holes cut out. You then have a stick with a mouse on it which you can poke through the holes. Just l… Read
30 Apr 2014
Portion Control Automatic Cat Feeder
Have you seen our Portion Control Automatic Cat Feeder in this months Your Cat. It really is a great automatic feeder and the perfect accessory now that summer is on it's way and holidays are on our mind. It is always a concern when pl… Read
24 Apr 2014


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This procedure shows how to target interneurons in the developing mouse forebrain by means of in utero electroporation. This technique was particularly efficient to achieve selective gene expression in interneuron subtypes destined to the superficial layers of the cortex.
Cite this ArticleCopy Citation | Download Citations
De Marco Garcia, N. V., Fishell, G. Subtype-selective Electroporation of Cortical Interneurons. J. Vis. Exp. (90), e51518, doi:10.3791/51518 (2014).
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The study of central nervous system (CNS) maturation relies on genetic targeting of neuronal populations. However, the task of restricting the expression of genes of interest to specific neuronal subtypes has proven remarkably challenging due to the relative scarcity of specific promoter elements. GABAergic interneurons constitute a neuronal population with extensive genetic and morphological diversity. Indeed, more than 11 different subtypes of GABAergic interneurons have been characterized in the mouse cortex1. Here we present an adapted protocol for selective targeting of GABAergic populations. We achieved subtype selective targeting of GABAergic interneurons by using the enhancer element of the homeobox transcription factors Dlx5 and Dlx6, homologues of the Drosophila distal-less (Dll) gene2,3, to drive the expression of specific genes through in utero electroporation.
The bulk of cortical GABAergic interneurons originate from two transient embryonic structures named the medial and caudal ganglionic eminences (MGE and CGE respectively)4. Parvalbumin and somatostatin expressing interneurons originate in the MGE whereas Calretinin (Cr), Vasointestinal peptide (VIP) and Reelin (Re) expressing interneurons originate from the CGE. These interneuron subtypes can be distinguished by their birthdates. MGE derived subtypes are born between embryonic day 9.5 (e9.5) and e16.55,6. In contrast, CGE derived interneurons are born from e12.5 through e18.5 with their production peaking at e15.56. The genetic targeting of this late born population, however, remains elusive.
The murine distal-less (Dlx) genes are exclusively expressed in the developing ventral forebrain3. GABAergic interneurons and striatal projection neurons but not cortical pyramidal cells express Dlx1,2,5, and 6 genes at early developmental stages3. Indeed, the Dlx genes are expressed in the MGE and CGE subventricular zone (SVZ) in all GABAergic progenitors. Expression of these genes becomes restricted to select subtypes at postmitotic stages7-9. Previous experimental evidence showed that the Dlx5/6 enhancer element allows for the selective targeting of GABAergic lineages in transgenic mouse models2. We tested the use of one of these enhancer elements in the context of episomal expression in the developing mouse brain. We sub cloned the Dlx5/6 enhancer element together with a minimal promoter and the enhanced green fluorescent protein (eGFP) in a bluescript (BS) backbone plasmid (Figure 1). We introduced the plasmid by means of in utero electroporation at e15.5 to selectively target Cr-, VIP and Re- subtypes3,8,10. Our technique allows for sparse electroporation, which facilitates the reconstruction of morphological features of singe cells. In addition, the exceptionally high levels of gene expression in cortical GABAergic neurons allows for functional studies. We carried out loss and gain of function studies using several wild type and dominant negative genes11.
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All animals were treated in accordance with the regulations and guidelines of the Institutional Animal Care and Use Committee of the NYU School of Medicine.
Mouse Strains
Swiss Webster female mice provided by TACONIC were used for these experiments. In order to specifically target superficial layer interneurons, e15.5 embryos were used.
Note: The plasmid used in this work (Dlx5/6.eGFP plasmid 3 µg/µl) was generated using standard cloning techniques. The eGFP cDNA was cloned into a Dlx5/6-Pmin-polyA plasmid. This plasmid is available upon request (firstname.lastname@example.com).
1. Preparation of Microinjection Pipettes
- Set the puller Heat #2 to 860.
- Place and secure the glass capillary near the filament. Check that the outer diameter (OD) of the pipette is roughly 30 μm.
- Press the "pull" button.
- Carefully remove the capillary. The bottom part is the needle. Discard the top part.
2. Anesthesia Procedure
- Prepare an isoflurane containing chamber. Note: Isoflurane is the preferred anesthetic due to its fast action and low incidence of side effects. Pentobarbital is another option; however, tolerance of this drug is variable and may lead to a higher mortality rate than isoflurane.
- Set the flow of isoflurane to 4-5% in 0.5-1 L/min O2.
- Make sure that the valve for the chamber is open whereas the valve for the head adaptor is closed.
- Place the pregnant mouse in the chamber.
- Allow the mouse to go under the effect of anesthesia. This will take about 2-3 min. Check the breathing rate. If the mouse begins hyperventilating, lower the dose of isoflurane.
- After the mouse is anaesthetized, promptly remove it from the chamber. Place it ventral side up on the heating pad and insert the head in the mask that will continue to supply isoflurane throughout the surgery. Make sure to switch the flow of isoflurane from the chamber to the tubing connecting the head adaptor piece. Put a drop of eye lubricant in each eye and close the eyes.
- Set the heating pad to 36 °C degrees to minimize hypothermia.
- Check for the absence of foot and tail reflexes by pinching the rear paws and the tail.
3. Surgery
- Cleanse the skin covering the abdomen with 70% ethanol.
- Use forceps to pull the skin upwards. After each surgery, wash all instruments with detergent and water, and sterilized at 72 °C O/N.
- Make a small vertical incision (about 2 cm) along the midline starting midway between the third and fourth pairs of mammary glands. Use fine surgery scissors. Take care to not damage the abdominal wall.
- Gently separate the skin from the peritoneum.
- Visualize the arteries and veins on the abdominal wall. Make another 2 cm vertical incision through the peritoneum (avoiding the vessels) to expose the abdominal cavity.
- Clamp the skin and abdominal wall on each side avoiding clamping the arteries. Note: If the arteries are accidentally punctured, sacrifice the animal immediately by performing cervical dislocation or isoflurane overdose.
- Pre warm the sterile PBS to 37 °C. Cover the clamps with PBS moist Kim wipes.
- Moisturize the exposed abdominal cavity with PBS. Repeat this step several times while performing the surgery.
- Using round forceps, gently pull the embryonic chain (e15.5) away from the cavity. Let the chain rest on the wet wipes. Start by exposing one half of the uterus first. Once the embryos in it are electroporated, bring it back inside, then work on the second half.
4. Electroporation
- Continue to use round forceps to manipulate the embryos.
- Visualize the lateral ventricles. The lateral ventricles run along the midline (Figure 1b).
- Add Fast Green (Stock: 10% w/v) to the DNA solution for a 1:20 mixture to visualize it during the injection. Prepare the DNA solution by dissolving the pellet obtained from a standard maxi-prep purification protocol in distilled water.
- Use pre pulled micropipettes with a plunger to inject 1 ml of DNA solution (Dlx5/6-eGFP, 3 µg/µl) with Fast Green. Cut the tip of the micropipette with a fine forcep #5 (Dumont) to leave 6 mm length from the beginning of the shoulder to the end of the tip of the needle before loading the pipette. Hold the head of the embryo with round tweezers. Enter the brain with the pipette at a 45° angle aiming for the lateral ventricle located near the midline. If the needle penetrates through the ventricle, slowly retract the pipette as you use the plunger to inject DNA. The ventricle should turn green when the solution begins to fill it. At this point, stop moving the pipette and inject 1 ml of DNA. Gently, withdraw the pipette.
- Set the CUY21 electroporator to five 45V pulses of 50 msec spaced by 950 msec.
- Use 5 mm paddle electrodes for e15.5 embryos. Dip the electrodes in PBS to ensure efficient current transmission.
- Place the electrode paddles around the embryo's head with the positive paddle on the ventricle containing the DNA solution. Slightly lower the positive paddle with respect of the negative one to create a ventral current through the ganglionic eminences. This manipulation will minimize ectopic expression in pyramidal cells.
- After placing the electrodes, deliver a pulse by pressing the foot pedal once.
- Remove the electrodes and wipe them clean. Repeat steps 4.6-4.9 with each embryo.
- After electroporating all the embryos, pour 3 ml of PBS into the abdominal cavity and return them to the abdominal cavity.
- First suture the abdominal wall with a curved needle and 5-0 silk suture and then the skin. Apply Lidocaine (4%) on the wound. Administer 120 mg/kg of Aspirin intra peritoneum for analgesia.
Note: The entire procedure should be performed in 20 min or less. It is advisable that beginners only electroporate a few embryos to keep the operation time short. Prolonged surgeries will decrease the survival rate of the embryos.
- Remove the animal from the anesthesia tubing and allow recovery on the heating pad on a paper wipe.
- Return animal to the cage, keep it on the heating pad at 37 °C and monitor the health status. The animals generally tolerate the surgery well and begin to move slowly soon after they are removed from the anesthesia tubing. If excessive bleeding or lethargy is observed, sacrifice the animal immediately by performing IACUC approved euthanasia procedures such as cervical dislocation or anesthesia overdose.
- Return cage to the vivarium. The females will give birth naturally.
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Representative Results
We adapted the in utero electroporation technique to achieve cell type specific targeting of maturing neurons. To drive the expression of eGFP in CGE-derived interneurons, we used the Dlx5/6 enhancer element and restricted our injections to e15.5, the stage when the majority of CGE-derived interneurons are generated. We carried out the analysis at P8 and P15 11(Figures 1 and 2). We confirmed the ventral origin of electroporated neurons by co electroporating a CAG-mCherry and a Dlx5/6-eGFP plasmids at equimolar concentrations at e15.5. In this experiment, we observed that only ventral progenitors located in the subventricular zone (SVZ) co expressed both proteins11. This spatio temporal expression of fluorescent proteins coincides with the normal developmental expression of the Dlx5 and 6 genes, which are not expressed in the ventricular but subventricular zone4. Furthermore, we assessed the GABAergic identity of Dlx5/6-mCherry electroporated interneurons in a GAD67-eGFP knockin mouse line. We found that the vast majority of electroporated neurons expressed GAD67, an enzyme expressed by all GABAergic cells11. In addition, we determined the subtype identity of electroporated interneurons by performing immunohistochemistry and electrophysiological analysis at P15. The pattern of expression of subtype specific markers was revealed by immunofluorescence in cryostat sections11 (Figure 2). We found that interneurons electroporated at e15.5 almost exclusively express NPY, Reelin, VIP and Cr, previously described CGE-derived interneuron markers6. Furthermore, the intrinsic electrophysiological properties of these neurons are in agreement with the one previously described in fate mapping studies6,11. All together, these results indicate that electroporation with the Dlx5/6 enhancer element at e15.5 selectively target CGE-derived interneuron subtypes.
Figure 1. Schematic representation of the electroporation experiments. a) Subcloning strategy for the Dlx5/6-eGFP plasmid. b) Diagram illustrating the experimental strategy.
Figure 2. Electroporated interneurons are delineated by the expression of CGE-derived subtype markers. A) CGE-derived interneurons electroporated with a Dlx5/6-eGFP plasmid. Note the sparse electroporation of diverse CGE subtypes. B) A VIP-expressing interneuron at P8. eGFP expression delineates the entire dentritic tree and axonal arbor of single neurons. C) An NPY-expressing interneuron at P8. NPY expression delineates neurogliaform cells. D) An NPY-expressing interneuron at P15. Scale bar A, 100 mm; B-D, 50 mm
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Limitations of the Technique
While this technique allows for cell autonomous analysis of cell processes, it is not suitable for population analysis. The electroporations are very sparse with less than a thousand cells electroporated per brain. As a consequence, the technique cannot be used to assess behavioral consequences arising from the genetic manipulation of CGE-derived interneurons.
While electroporations carried out at e13.5-e14.5 target MGE-derived subtypes, the efficiency is low10. We speculate that the Dlx5/6 enhancer is less active in postmitotic MGE subtypes at least in the context of episomal expression.
Significance with Respect to Existing Methods
The technique represents advancement from previously electroporation methods12,13 for the study of interneurons. Due to their relative scarcity and ventral embryonic origin, this population of neurons has proven difficult to target. Our technique provides the means to carry out cell-autonomous analysis of CGE-derived interneurons. The high levels of eGFP expression allow for the visualization and analysis of single interneurons.
Future Applications
By combining the use of specific plasmids and genetically modified mouse lines it is possible to achieve temporal and spatial control in the expression of genes of interest. For example, we used a Dlx5/6-Tta plasmid to turn on the expression of tetO inducible transgenes. In these experiments, we were able to silence the transgene expression by administrating Doxycycline8. We are currently developing a protocol to use the technique in combination with the CreER system.
Critical Steps within the Protocol
To achieve efficient electroporation and survival, try to avoid excessive manipulation of the embryos and/or organs. In addition, avoid pinching arteries and veins. The mouse will quickly become hypovolemic if bleeding occurs. After the electroporation is completed, place the embryos and organs in the same positions where you found them to avoid creating kinks that could cause hypoxia. Aim for 20 min or less surgery time. Moisturize the abdominal cavity with PBS numerous times during the surgery. During the electroporation, try to puncture the ventricle only once with the capillary needle. Repetitive puncturing will decrease the survival rate. After the training period, the survival rate should be 80% or higher.
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The authors have nothing to disclose.
We are grateful to Lihong Yin for technical assistance. NVD is a recipient of a NARSAD Young Investigator Award and is also supported by grants from NIH (5 K99 MH095825-02). Research in the Fishell lab is supported by the National Institute of Health, National Institute of Mental Health (5 R01 MH095147-02, 5 R01 MH071679-09), National Institute of Neurological Disorders and Stroke (5 R01 NS081297-02, 1 P01 NS074972-01A1) and the Simons Foundation.
|Catalog Number
|Electroporator with pedal
|Protech International
|5 mm paddle electrodes
|Protech International
|Heating pad
|Kent Scientific
|Sutter Instruments P30 Puller
|Sutter Instruments
|Fluovac Anesthesia Systems
|Harvard Apparatus
|Delicate Operating Scissors 4.75" Straight Sharp/Sharp
|5-0 Silk Black Braid 18" C-1 Box 36
|Micro Clip Applying Forceps 5.5"
|2 Clamp scissors
|Holding forceps
|Fine Science Tools
|Glass capillary tubing
|Borosil 1.0 mm OD x 0.75 mm ID
|Fast Green
|Sterile PBS
|Life Technologies
- Fishell, G., Rudy, B. Mechanisms of inhibition within the telencephalon: "where the wild things are". Annual review of neuroscience. 34, 535-567 (2011).
- Stenman, J., Toresson, H., Campbell, K. Identification of two distinct progenitor populations in the lateral ganglionic eminence: implications for striatal and olfactory bulb neurogenesis. J Neurosci. 23, 167-174 (2003).
- Eisenstat, D. D., et al. DLX-1, DLX-2, and DLX-5 expression define distinct stages of basal forebrain differentiation. J Comp Neurol. 414, 217-237 (1999).
- Batista-Brito, R., Fishell, G. The developmental integration of cortical interneurons into a functional network. Curr Top Dev Biol. 87, 81-118 (2009).
- Miyoshi, G., Butt, S. J., Takebayashi, H., Fishell, G. Physiologically distinct temporal cohorts of cortical interneurons arise from telencephalic Olig2-expressing precursors. J Neurosci. 27, 7786-7798 (2007).
- Miyoshi, G., et al. Genetic fate mapping reveals that the caudal ganglionic eminence produces a large and diverse population of superficial cortical interneurons. J Neurosci. 30, 1582-1594 (2010).
- Bulfone, A., et al. The mouse Dlx-2 (Tes-1) gene is expressed in spatially restricted domains of the forebrain, face and limbs in midgestation mouse embryos. Mechanisms of development. 40, 129-140 (1993).
- Bulfone, A., et al. Spatially restricted expression of Dlx-1, Dlx-2 (Tes-1), Gbx-2, and Wnt-3 in the embryonic day 12.5 mouse forebrain defines potential transverse and longitudinal segmental boundaries. J Neurosci. 13, 3155-3172 (1993).
- Robinson, G. W., Wray, S., Mahon, K. A. Spatially restricted expression of a member of a new family of murine Distal-less homeobox genes in the developing forebrain. The New biologist. 3, 1183-1194 (1991).
- Close, J., et al. Satb1 is an activity-modulated transcription factor required for the terminal differentiation and connectivity of medial ganglionic eminence-derived cortical interneurons. J Neurosci. 32, 17690-17705 (2012).
- De Marco Garcia, N. V., Karayannis, T., Fishell, G. Neuronal activity is required for the development of specific cortical interneuron subtypes. Nature. 472, 351-355 (2011).
- Petros, T. J., Rebsam, A., Mason, C. A. In utero and ex vivo electroporation for gene expression in mouse retinal ganglion cells. Journal of visualized experiments : JoVE. (31), (2009).
- Walantus, W., Castaneda, D., Elias, L., Kriegstein, A. In utero intraventricular injection and electroporation of E15 mouse embryos. Journal of visualized experiments : JoVE. (6), e239 (2007).
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Finding the right day care center in Miami can be a challenge if you don't know where to start. As the largest city in Florida, Miami has dozens of child care options to choose from. HiMama has compiled this list of quality centers to help narrow dowon your search. Our top picks are based on client reviews, geographic area, and program offerings.
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Fairly unique among war films for its nearly seamless incorporation of stock footage into its fictional narrative (amounting to approximately 25% of the overall running time, and culled from the roughly 18,000 hours worth of World War II footage preserved at the Imperial War Museum in London), Overlord is at once exacting in its historical specificity and timeless in its evocation of life senselessly lost. Thomas Beddows (Brian Stirner) is still a minor when he embarks from his English home for the mounting effort against Germany. He arrives to basic training late, delayed by the London Blitz, and the bulk of the film meanders with him as he's shuffled from base to base with little to do other than think about his likely, inevitable death. Ultimately, he perishes instantly from a stray bullet before he even gets out of the boat at Normandy. From its opening in darkness, to its slipstream tapestry of wartime footage, to our surrogate's quiet ruminations about life, death, and everything in between, Stuart Cooper's film achieves an unlikely fusion of the gorgeous and the despairing—an anguished look at the devastation wrought even by a morally justified war. By the end, that our everyman's death is shown to be essentially useless is practically beside the point.
Utilizing antique camera lenses to approximate the look of the combat footage around which the rest of Overlord was conceived, Cooper's film achieves an ethereal quality that seems to transcend time, a quality aided immensely by the use of black-and-white 35mm, unknown actors, and an effectively simple story. The performances are reserved and awkward, with a raw sincerity that speaks to a careful balance between expression and function, or, if you will, free will and determinism—all the better to mirror the fact that the countless individuals they're representing throughout history also went about similar paths and to similar fates, unsure of the roles they played as they gave of themselves to a cause beyond their understanding. This is a British film about a British soldier, but it could just as easily be any soldier's story. The imagined and the real are blurred in Thomas's frequent return to the daydreamed image of a soldier perishing while charging an unseen enemy; so, too, does the film achieve a dreamlike effect as the newly shot footage meshes with haunted images of real bombs falling toward unseen targets, real explosions punctuating the cloudy terrain of a nighttime sky, and real bullets strafing living targets, as seen from the cockpits of their attackers. John Alcott's cinematography is a carefully tempered mixture of lightly poetic visuals and matter-of-fact coverage, faithfully recreating the quotidian artistry captured by so many wartime cameramen forced to keep the camera rolling as hell unfolded around them.
The film's title refers to the eponymous military operation that saw so many lives lost on D-Day, but in the filmmaker's decision to strip the work of historical bullet points, it takes on a more cosmic tone, as if a great (or terrible) entity, ever-present, were responsible for the horrors on display and the even greater horrors suggested. The source of man's inhumanity to man, and its consequences, go beyond Thomas's ability to understand them, and indeed, the film deliberately eschews any kind of larger summation of its themes and content in favor of a fitting ambivalence, much like how its doomed hero eventually gives up and accepts his place in the world despite his relative obliviousness to it. The war machine is a presence both figurative and literal here, from the nightmarish, dragon-like contraptions that ravage a beach, chewing up barbed wire and scouring for undetonated mines, to the unseen maneuvers that separate our taciturn protagonist from the girl (a radiant Julie Neesam, poignantly referred to only as "the girl") he meets during a rare respite from his duties. In its alternating sense of rage, apathy, and despair, Cooper's film is among the most honest films ever made about the effects of war.
Because of Overlord's melding of raw combat footage and newly shot sequences, there are inevitable fluctuations in image quality, but even if one doesn't discount the obvious wear and tear that accompanies the former material, this is still a striking 1080 transfer. Remastered without any noticeable impact on the roughly hewn integrity that contributes to the film's eerie melancholy, grain has been richly maintained, with minimal noise. Sound, here presented only as an English LPCM 1.0 track, is similarly excellent despite its technical simplicity. Paul Glass's score is nicely balanced amid the dialogue and sound effects, which remain crisp despite the mono track's limitations.
A small bounty of worthwhile supplements helps lend context to the exquisitely archetypal Overlord. The 23-minute "Mining the Archives" details Stuart Cooper's initial intentions of making a documentary and the scope of wartime footage available to him. The director also provides an introduction to the "Soldiers' Journals" feature, in which the diaries of two sergeants, Edward Robert McCosh and Finlay Campbell, are movingly read aloud; several lines from the film found their genesis in these passages, verbatim. The roughly 10-minute long "Capa Influences Cooper" examines the surviving work of wartime photographer Robert Capa, whose shots from Omaha Beach on D-Day convey the horror of that battle better than many motion pictures could ever aspire to. Additionally, there's an original theatrical trailer, an insightful feature-length commentary track from Cooper and lead actor Brian Stirner, and an illustrated booklet with an essay from film critic Kent Jones and passages from the film's novelization. Rounding out the set are three short films: the historical anti-Nazi parody Germany Calling, seen within Overlord itself, as well as the historical newsreel short Cameramen at War and Cooper's own A Test of Violence, a 1969 short that repurposes the paintings of Juan Genovés, whose depictions of violence and oppression were an obvious influence on Cooper's later work.
Meriting comparisons with similarly themed works by everyone from King Vidor and Lewis Milestone to Steven Spielberg and Terrence Malick, Stuart Cooper's film now has the chance to find the audience it always deserved. Criterion does right by the little-seen masterpiece.


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Riddle me this, bat-friends. What has one head, one tail, and ten legs?
K is for Kukuweaq.
The Kukuweaq comes from Inuit folklore. The beast is a polar bear with ten legs. Yes, that's right – ten of them. I wouldn't want to be chased by a four-legged polar bear, much less ten!
The bear's ten legs help it travel long distances on both land and water. Some sources also claim that the beast is as large as an iceberg and almost impossible to kill. But in one folktale, a man does bring down one of these creatures.
Two neighbors settled in for the winter. One had stashed away a walrus to feed him during the harsh months, while the other (Kucriak) had nothing. Kucriak's neighbor refused to share the walrus with him, so Kucriak went out in search of food. He came across the Kukuweaq's den and killed it by stabbing his harpoon in the Kukuweaq's eye. Unlike his greedy neighbor, Kucriak shared the bear's meat with his entire village, saving them from starvation. Of course, his neighbor was embarrassed, since he had not shared his walrus.
I think the message in this particular folktale is clear – one must be generous towards his or her community. This is a significant cultural need, especially in isolated societies where resources are scarce. Hawaiian cultures have similar story in "The Calabash of Poi," as do the Australians in "How the Kangaroo Got Her Pouch."
What I'd like to know more about is why the Kukuweaq has ten legs – aside from the fact that the creature can travel long distances. The scientific term for having multiple limbs is polymelia. Many cultures have mythological creatures with polymelia, such as the Hindu Kali (a goddess, not a creature) and the Greek Hekatonkeires. I've been looking for more information about why this is so significant, but have failed to find anything yet. Let me know if you have a hint! (And I'll let you know if I find something.) Aside from arms typically connoting strength, I'm at a loss.
Robert F. Spencer. The North Alaskan Eskimo: A Study in Ecology and Society. (1959)


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Little Known Facts About hotel menarik di penang.
With clean up shorelines, swaying palms and crystal very clear waters, Langkawi is well-frequented by travellers worldwide for its island-hopping maritime actions which include snorkelling, scuba diving and also shark feeding. Showcasing several of the most postcard-excellent beaches in Malaysia, Langkawi is property to pristine rainforests and marine parks, plus the better part about its a lot of resorts is the fact that attendees are in no way that considerably from your Beach front.
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pasal rim saya puas hati, tapi yang hati tak puas ialah saya espect tayar yang elok skit sekurang2nya silverstone evol8. Tapi saya dapat tayar wanli(sunny) sahaja.
Participation in This system demands Assembly a particular set of standards and requires feed-back from past friends into account.
The Philippines presents almost everything from mountains and volcanoes, to bustling towns, seashores, leading scuba diving websites and in many cases Planet Heritage cities. Manila is the Filipino money plus a much frequented metropolis in its individual proper.
With a few really wonderful coastal stretches and shorelines, Malaysia enjoys a healthful tourist industry.
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Comprised of in excess of seven,000 islands, the link densely populated Republic with the Philippines is called house by a lot more than 90 million individuals. The tropical climate on the Philippines is particularly scorching from March to Might, even though among December and February, the weather conditions is substantially cooler.


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Hyndland Physiotherapy Clinic
170 Hyndland Road
Tel: 0141 339 1329
Monday to Friday 8am - 6pm
Late night, weekend and home visits also available.
Back pain
Back problems affect most people at some point in their lives and come in many forms. Lower back pain is second only to the common cold as a cause of lost days at work. It can be debilitating affecting the sufferer's life. Common back pain causes include nerve and muscular problems, degenerative disc disease, and arthritis.
The spine is made up of 24 separate bones called vertebrae, plus the bones of the sacrum and coccyx. Between the vertebrae are discs that act as shock absorbers and allow your spine to bend. Your spinal cord threads down through the central canal of each vertebra, carrying nerves from your brain to the rest of your body. Sometimes these nerves get irritated when the disc presses on the nerve. Nerve pain can radiate into the legs in conditions such as sciatica.
Sciatica is the name given to any sort of pain that is caused by irritation or compression of the sciatic nerve. The sciatic nerve is the longest nerve in the body. It runs from the back of your pelvis, through your buttocks, and all the way down both legs, ending at your feet. When something compresses or irritates the sciatic nerve, it can cause a pain that radiates out from your lower back and travels down your leg to your calf. Sciatic pain can range from being mild to very painful. Nerve pain can also radiate down the arm causing pain and discomfort.
Other forms of back pain such as stiffness and muscle spasm; which is the body's protective response and is reflex in nature, can originate from poor posture, weakness or imbalance in musculature or injuries from lifting and bending. All of these conditions can be relieved with physiotherapy treatment, advice on management and massage therapy.
Good back care can greatly reduce your risk of getting low back pain. To look after your back, make sure you take regular exercise, walking and swimming are particularly beneficial. Try to keep your stress levels to a minimum. Bend from your knees and hips, not your back and maintain good posture; keep your shoulders back and don't slouch.


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If you have applied for Walmart credit card, you will be notified via email. The email will indicate you as a pre-qualified Walmart credit card holder. use this website to apply for the Walmart credit card walmartcardoffer.com/prescreen. In the email, you have received is a guide, guiding you how to further the process to get a Walmart credit card. There is an applicated attached to the email, fill it and submit. Beware of the deadline!
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Live from Passim in Cambridge, MA (USA). 8pm.
Live from Passim in Cambridge, MA (USA). 8pm.
I don't see it listed for tonight...?
2007 Weber Custom Elite "old wood"
2017 Ratliff R5 Custom #1148
Several nice old Fiddles
2007 Martin 000-15S 12 fret Auditorium-slot head
Deering Classic Open Back
Too many microphones
Matt just informed us there will be no Concert Window show tonight after all. Technical issues. 8-(
Last edited by Michael Neverisky; Nov-08-2017 at 7:35pm.


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All posts tagged "FASHION"
Dawn Richard Channels Grace Jones for Annex Man Magazine
I am sorry but I am here for every photo Dawn Richard takes… WERK!
Supreme 2013 Fall/Winter Collection
With summer just about over, it's time to find some new threads for the new school year. Supreme...
Is Kim Kardashian Releasing a Baby North West Inspired Fashion Line?
Will new mom Kim Kardashian launch a baby North West inspired fashion line? Designer Lloyd Klein says there's...
FIERCE! Rita Ora Is the New Face of DKNY!
British songstress Rita Ora took over the Big Apple for a impromptu photo campaign for DKNY!
Been Trill x SSUR Unveil Their Collaborative Snapback Collection
Summer might be almost over, but it is never too late to pick up some new snapbacks. Been...
Karreuche Gets Rocker Chic For New Karmaloop Campaign
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Nicole Kidman Covers German Vogue August 2013
Academy Award winner Nicole Kidman graces the cover of German Vogue with two very different photos. Kidman was...


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One of a child's most lasting memories is often of the first fish they caught. Many programs help parents and children catch their first fish at various fun fishing events. However, the Florida Fish and Wildlife Conservation Commission's (FWC) Fishing Camp program goes far beyond by helping young people learn a healthy lifetime sport and the importance of conservation stewardship.
The freshwater fishing and basic boating skills camp is based on the successful pilot program developed by FWC at Joe Budd Youth Conservation Center, near Tallahassee. Beginning in June 2000, about 100 youngsters have attended the camp each summer, and research surveys have shown the vast majority continues to enjoy recreational angling and to feel more connected to nature.
In 2011, Florida's Governor recognized Rae Waddell and Steve Marshall, who created this program, for their efforts to work with local partners to provide healthy, conservation-minded opportunities for Florida's youth. Fishing license dollars, and more specifically voluntary donations to youth fishing and hunting programs that many anglers make when they buy their licenses (MyFWC.com/License) help fund these camps.
The FWC's Florida Youth Conservation Centers Network has established partnerships with over a dozen City and County Recreation programs, and multiple not-for-profit organizations, to bring the Fishing Camp program to local communities. Efforts to expand these programs and get more children involved in active nature-based recreation not only provide an enjoyable introduction to this classic American tradition, but also benefit their physical and mental health and endow a sense of stewardship for our natural resources.
Robert Carmona, a Park Manager with Miami-Dade County Parks, said: "Our inaugural fishing camp was a success not only because we operated at capacity, but also because we were able to introduce children to angling and instill in them a respect for the resources." Through adding fishing camp to Miami-Dade's summer offering they have introduced hundreds of children to a new lifetime sport that is affordable and gets them active in nature. "Part of Miami-Dade Park's mission is to create outstanding natural, recreational opportunities for this and future generations. By teaching about fish, water, and healthy environments, we instilled a sense of responsibility for the environment that we hope will translate into advocacy for the parks department and green spaces in general," said Carmona.
FWC continually seeks partner groups who are committed to working with youth and/or families to provide quality programs and who will help document the long-term success of these educational efforts. In return, FWC provides a Fish Camp Implementation Template, on-site staff training and assists with purchasing camp essentials that are expected to last several years.
If you have staff or volunteers and facilities to support a Fishing Camp in your local community and are interested, please contact: Steve Marshall, Florida Youth Conservation Centers Network, Florida Fish and Wildlife Conservation Commission, phone (561)292-6050, Email: email@example.com.
FWC Facts:Although designed primarily for women, Becoming an Outdoors-Woman workshops are a great way for anyone 18 or older to learn a variety of outdoor skills.
Learn More at AskFWC
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The Fantail is a popular breed of fancy pigeon. It is characterised by a fan-shaped tail composed of 30 to 40 feathers, abnormally more than most members of the pigeon family, which usually have 12 to 14 feathers.The breed is thought to have originated in India, China or Spain. There are several subvarieties, such as the English Fantail, the Indian Fantail, and the Thai Fantail.
There is a feather mutation called Silky that gives an interesting lace effect to a Fantails tail feathers. Fantails with this mutation are known as Silky or Lace Fantails.
Fantails are often used by pigeon flyers in the training of racing pigeons and Tipplers. They are used as droppers in that they are placed on the loft landing board as a signal to the flying birds to come in and be fed.


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The Vietnamese market in Malesice on the edge of Prague mostly sells cheap clothes and goods. But, there are a few grocers like this one selling everything you might possibly imagine in terms of fresh herbs and ingredients to make Asian food. We loved this place.
DPNMalesiceMalesice marketVietnamese foodVietnamese marketfoodfood marketfruitsherbsmarketvegetablesMalešicePrague 10PragueCzech Republic


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*** Honor Mission*** Paul Bradford
U.S. Army/Fire Fighter
Seymour, CT
THU JUL 17th 0930hrs
Patriot Guard and associates,
Sadly we are called to stand for Paul Bradford, who served our country honorably in the U.S. Army and as a Volunteer Fire Fighter, may he rest in peace.
Paul was a Southbury Fire Department volunteer and served as a Armor Crewman in the US Army.
We will stand a Flag Line at the cemetery.
ASC Mac McArthur will be RCIC and have flags on site.
0845hrs (staging)
Thursday July 17th
St Augustine Cemetery
Seymour, CT
I could not locate a good physical address so please source directions from your location. It is right off RT 8,exit 21 if coming North.
300 Ceder St. (approximatelly)
God Bless America, Our Troops and 1st Responders!
Gregg "Flag 7" Barratt
Patriot Guard Riders
Connecticut State Captain
Director, Honor and Remember of CT
"Standing for Those Who Stood for US"!


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Here's the review of Pirates and Nobles by IGN.
Less than six months ago, Electronic Arts released The Sims Medieval. Despite being based on the Sims 3 engine, it was a reinvented Sims experience: you were no longer managing personal needs and taking care of a family, but living in a small kingdom and completing quests. It was an evolution of one of my favorite games of all time.
Unfortunately, the same can't be said of the first expansion, Pirates & Nobles. The bullet points read like a standard expansion for any of the classic Sims games: new traits, new clothes, new objects, and new social interactions. We've also got a new treasure hunting mechanic if you're really bored.


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Posts Tagged
stanford cardinal
Ep. 862 – John Roberson (CEO, Advent)
Podcast: Play in new window | Download
http://traffic.libsyn.com/sportstaopodcast/TaoEp0862.mp3Podcast: Play in new window | DownloadSubscribe: Android | RSSThe core principles of design and branding are discussed beyond the idea of logo, focusing on environment for both the staff member and the fan. John Roberson covers some of the dynamics of what his company, Advent, has done in the


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PageSeeder file system
Directly interacting with the file system is not advised! Use the programming or user interfaces to move files in or out of PageSeeder.
The PageSeeder file system is inherited from the operating system hosting the PageSeeder server. This is important because users may move files from a desktop environment that is not case-sensitive to a server environment that is. Or from a server that supports long folder names to a desktop that doesn't.
This is rarely a serious problem, but it can lead to confusion.
Created on , last edited on


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Autonomous Cars and Transport Innovation Seminar
Last week Professor Nick Reed from the UK Transport Research Laboratory gave a fascinating presentation on Autonomous Cars and Transport Innovation at Les Cotils.
Nick gave us an insight into the trails they are running on autonomous vehicles including shuttle transport on the Meridian, valet parking and urban deliveries. He also discussed the benefits to Guernsey including less traffic and congestion, less pollution, safer roads, access for disabled travelers, adaptable routes and travel times and more. Nick summarised the presentation up saying that there are exciting opportunities for Guernsey to play a role in future transport.
Chamber President Tony Rowbotham commented on the seminar, "A fascinating presentation and the advantages for Guernsey are significant. Not just for our own transport system, but as an island how we can be market leaders in support to this rapidly growing sector with insurance, financing and technology all being areas where the island can add value."
We would like to thank our speaker Nick.
UK Transport Research Laboratory Presentation


text: Free Features | November 01, 2006Telepractice Program for Dysphagia: Urban and Rural Perspectives From Kansas Jill Georges; Norbert Belz; Kristy Potter Author Notes Jill Georges, is director of rehabilitation services and manager of speech-language services at the University of Kansas Hospital. Contact her by e-mail at email@example.com Georges, is director of rehabilitation services and manager of speech-language services at the University of Kansas Hospital. Contact her by e-mail at firstname.lastname@example.com.× Norbert Belz, is project manager of Telehealth Clinical Services at the University of Kansas Medical Center. Contact him by e-mail at email@example.com Belz, is project manager of Telehealth Clinical Services at the University of Kansas Medical Center. Contact him by e-mail at email@example.com.× Kristy Potter, is a speech-language pathologist who provides contract and private therapy services to south central and southeastern Kansas. Contact her by e-mail at firstname.lastname@example.com Potter, is a speech-language pathologist who provides contract and private therapy services to south central and southeastern Kansas. Contact her by e-mail at firstname.lastname@example.com.× © 2006 American Speech-Language-Hearing Association Article Information Swallowing, Dysphagia & Feeding Disorders / Telepractice & Computer-Based Approaches / Features Features | November 01, 2006 Telepractice Program for Dysphagia: Urban and Rural Perspectives From Kansas The ASHA Leader, November 2006, Vol. 11, 12-13. doi:10.1044/leader.FTR5.11152006.12 The ASHA Leader, November 2006, Vol. 11, 12-13. doi:10.1044/leader.FTR5.11152006.12 View Article Figures Tables Supplemental Data Supplements Multimedia Share Email Twitter Facebook Pinterest Tools Get Citation Citation Georges, J., Belz, N., & Potter, K. (2006). Telepractice Program for Dysphagia: Urban and Rural Perspectives From Kansas. The ASHA Leader, 11(15), 12-13. doi: 10.1044/leader.FTR5.11152006.12. Download citation file: RIS (Zotero) EndNote BibTex Medlars ProCite RefWorks Reference Manager © 2018 American Speech-Language-Hearing Association × Alerts User Alerts You are adding an alert for: Telepractice Program for Dysphagia: Urban and Rural Perspectives From Kansas You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account The alert will be sent to: Confirm × Sign In or Create a free account to receive alerts. × The Urban Perspective by Jill Georges and Norbert Belz When the University of Kansas Hospital was asked to become involved in remote, interactive videoswallow evaluations, we saw it as an opportunity to improve services in rural areas. We first reviewed the literature and found that telepractice had been used for a variety of speech-language pathology and audiology services, but the only published report of utilizing telemedicine for videoswallow evaluations was by Perlman and Witthawaskul (2002), who utilized an Internet system for real-time, remote, interactive evaluation of swallowing. Our technology utilized a videoflouroscope that attached directly to a Polycom F/X system at the remote site. This project was funded by a three-year federal grant from the Office for the Advancement of Telehealth (OAT). The grant helped establish services at the sites in which we conducted the swallow studies. The clinic/hospital provided the fluoroscope and radiology equipment. The original grant covered the telecom monthly costs as well as usage during the evaluation (typically $30–$50/hour), and provided a stipend for our professional time ($75/patient) and for the site coordinator. We were satisfied that the quality of the videoswallow study would be comparable to one obtained in our own fluoroscopy suite, but were reluctant to become involved unless we were sure that preparation and follow-up would be adequate. We knew that many of our patients would be elderly nursing home residents. We were fortunate that the co-author of this article, Kristy Potter, is a speech-language pathologist (SLP) who was able to complete clinical swallow evaluations, partner with us in completing swallow studies, and provide follow-up. We also wanted to ensure that our venture was within our scope of practice and licensure laws. ASHA's Code of Ethics (2001) states that individuals may practice telemedicine where not prohibited by law. The National Council of State Boards of Examiners for Speech-Language Pathology and Audiology (NCSB) issued a statement in 2004 that SLPs and audiologists must be licensed in the state in which the service is provided. This limitation was not an obstacle as both sites are in Kansas. The remote, interactive swallow studies were well received at the remote site. Both clinicians were comfortable with the technology and studies indicated good acceptance by patients. A unique aspect of our experience was the opportunity to have certified SLPs at both sites, greatly enhancing the service we provided. Involving two providers also meant that our purpose evolved from simply providing videoswallows in a remote area to also training the clinician to perform this service independently. Unfortunately, the grant that funded this project recently expired. In addition, the remote hospital—in a rural town that has been struggling economically—recently closed. The steady number of patients in need of this service must now be transported two hours to receive a videoswallow. We were fortunate to have an experienced radiology technician through most of this project, but he eventually resigned to seek employment opportunities elsewhere. Fortunately, the facility still has a radiology suite available for a future radiologist/technician. The cooperative venture increased the skill and confidence of the clinician at the local site, who now can complete swallow studies independently, and provided an invaluable diagnostic service to patients in an underserved rural area of Kansas. The Rural Perspective by Kristy Potter After completing graduate school, I accepted a position in a rural, remote area in Kansas. It was 1992, and speech-language pathology services were just emerging in small hospitals and long-term care facilities. Although I knew I was licensed and qualified to diagnose and treat my patients, feeling competent to do so was another story. Yes, I had completed a respected, accredited graduate program; however, instead of feeling confident, I was feeling more of the usual (and may I now say expected) "I'm not quite sure what to do" angst of a brand-new Clinical Fellow. This feeling was most pronounced in the area of dysphagia. I was exposed to dysphagia in my practicum, but it was limited at best. I had some instruction on diet modification and I watched a few videotaped modified barium swallows (MBS), but I had no first-hand experience establishing and implementing a treatment program for dysphagia. Suddenly, I was the sole resource in several facilities with patients with dysphagia. In our remote area, MBS evaluations were typically not available, so I read what I could and tried to consult with other clinicians. I, however, was one of only a few speech-language pathologists (SLPs) practicing in a rehab setting, and certainly the only one in my facilities. Experience was often the result of trial and error. The challenges I faced in Kansas were typical of SLPs practicing in rural areas. I spent most of my career providing services to rural and remote facilities without equipment, support staff, or established competence to perform videoswallows. My only assessment tool was a clinical bedside evaluation, which research tells us may miss aspiration 40%-60% of the time (Logemann, 1998; Splaingard, Hutchins, Sulton, & Chaudhuri, 1988). As the use of MBS became more widespread, I began to refer my patients to a hospital that could perform this service. Travel to the hospital imposed great hardships (in my current position, a 100-mile round trip). Three years ago, I was invited to participate in the first remote, interactive telemedicine videoswallow evaluation with the University of Kansas Medical Center (KUMed). Since that time we have routinely utilized telepractice to complete videoswallow evaluations. Using telepractice, the SLP at KUMed is able to view swallowing evaluations transmitted from our rural site. The videoconferencing device attaches directly to the digital fluoroscope, providing a live, radiologic image that is seen by the SLP at the urban site as effectively as if she were seeing it on the fluoroscope in her own facility. Except for the technology involved, our procedures are similar to those used during any videoswallow evaluation. I receive a consult, complete a clinical swallow evaluation, and forward medical information and clinical swallow evaluation results to the clinician at the Center for Telemedicine and Telepractice at KUMed. During the instrumental swallow evaluation, I provide the "hands on" portion of the swallow study. The SLP at KU Hospital is able to converse with me and the radiology technician and observe the patient before the fluoroscopy. Once the swallow study is initiated, the fluoroscope is attached to the Polycom, allowing the SLP to directly observe the study. Meanwhile, she and I are able to discuss the procedure and our observations, and problem-solve as indicated. After the study, we discuss the case and finalize recommendations. I provide follow-up with caregivers and initiate treatment when indicated. The benefits of telepractice for videoswallow evaluations are many and far-reaching: it provides rural, underserved areas with access to an academic medical center and both patient and provider benefit from the knowledge and expertise of a host of specialized medical professionals. Patients in rural areas may wait days for an MBS; in most cases, telemedicine can be scheduled the next day. Travel and waiting time at the site are also decreased, thus reducing fatigue and improving accuracy of results. Patient follow-up is also enhanced through timely discussion of results and recommendations. In addition, the interactive nature of the evaluation provides an opportunity for professional growth and skill development. Practicing in a rural environment is often rather isolating with very few opportunities to interact with colleagues. Telepractice offers a way to expand one's knowledge base while developing mentor and peer relationships that would otherwise not be available. References Logemann, J. A. (1998). Evaluation and treatment of swallowing disorders (2nd ed.). Austin, TX: Pro-Ed.Logemann, J. A. (1998). Evaluation and treatment of swallowing disorders (2nd ed.). Austin, TX: Pro-Ed.×Perlman, A. L., & Witthawaskul, W. (2002). Real-time remote telefluoroscopic assessment of patients with dysphagia. Dysphagia, 17(2), 162–167. [Article] [PubMed]Perlman, A. L., & Witthawaskul, W. (2002). Real-time remote telefluoroscopic assessment of patients with dysphagia. Dysphagia, 17(2), 162–167. [Article] [PubMed]×Splaingard, M. L., Hutchins, B., Sulton, L. D., & Chaudhuri, G. (1988). Aspiration in rehabilitation patients: videofluoroscopy vs. bedside clinical assessment. Archives of Physical Medicine and Rehabilitation, 69, 637–640. [PubMed]Splaingard, M. L., Hutchins, B., Sulton, L. D., & Chaudhuri, G. (1988). Aspiration in rehabilitation patients: videofluoroscopy vs. bedside clinical assessment. Archives of Physical Medicine and Rehabilitation, 69, 637–640. [PubMed]× 0 Comments Submit a Comment Submit A Comment Name Comment Title Comment Could not validate captcha. Please try again. You have entered an invalid code Submit Cancel Thank you for submitting a comment on this article. Close This feature is available to Subscribers Only Sign In or Create an Account × FROM THIS ISSUE November 2006 Volume 11, Issue 15 ‹ Issue › Peruse what's new on Leader Live! Why Use Literary Interventions for Diverse Populations When Sam Found Language Tips for Making a Successful Cleft Palate Team Referral ADVERTISING DISCLAIMER|ADVERTISE WITH US Related Articles SIGs Elect New Leaders The ASHA Leader, September 2015, Vol. 20, 59. doi:10.1044/leader.AN7.20092015.59 SIGs Welcome New Coordinating Committee Members The ASHA Leader, October 2013, Vol. 18, 62. doi:10.1044/leader.AN14.18102013.62 SIG Elections Close June 17 The ASHA Leader, June 2015, Vol. 20, 59. doi:10.1044/leader.AN3.20062015.59 Promoting Social Connections in a Synchronous Telepractice, Aphasia Communication Group Perspectives of the ASHA Special Interest Groups, October 2018, Vol. 3, 32-42. doi:10.1044/persp3.SIG18.32 Getting Started in School-Based Speech-Language Pathology Telepractice Perspectives of the ASHA Special Interest Groups, October 2018, Vol. 3, 21-31. doi:10.1044/persp3.SIG18.21 ADVERTISING DISCLAIMER|ADVERTISE WITH US Related Topics Swallowing, Dysphagia & Feeding Disorders Telepractice & Computer-Based Approaches


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If the following might suggest a little too great a resemblance between Peter Death Bredon Wimsey and Bella Swann, well, just call it a jeu d'esprit (that's what I always say).
Art for art's sake
Lord Peter had often remarked disparagingly on the habits of amateur murderers who failed to pay sufficient attention to the detail of their crime, and thus found themselves discovered. It was a pity, thought Bunter, pounding the ashes with the poker as he tidied the library at the end of the night, that Lord Peter did not apply the same attention to his personal affairs. The mood produced by a surprisingly successful lunch with Miss Vane, followed by a particularly convivial evening at his club marking the fortieth birthday and upcoming nuptials of Mr Arbuthnot, had not been conducive of caution, with the result that half-charred scraps of paper covered with inky scribbles reading Harriet Wimsey, Lady Peter Wimsey, Harriet, Lady Peter Wimsey, Lord and Lady Peter Wimsey and, repeatedly over a whole sheet, embellished with little curlicues and what were presumably meant to be turtle doves, I love Harriet Vane, were left for the service flats' staff to discover in the morning and, knowing Evelyn as Bunter feared that he did, giggle over to the detriment of their duties. Now that the trial was over, and the lady's innocence conclusively proved, this might have been merely embarrassing had the writing not also been accompanied by his lordship's attempts at a portrait of the object of his affections. His lordship had many talents, but, Bunter reflected, the artistic depiction of the human form was not among them. Nor did the little bubbles of speech above the figures' heads add to their dignity. As for this one, screwed in a ball on top of the others and barely singed, once the paper had been sufficiently smoothed and the traces of India-rubber dusted away to reveal the fine detail with which the artist had evidently struggled, no wonder his lordship had thrown the lot so hastily on the fire and hurried away to the bedroom if that was what was on his mind. One couldn't fault the artist's enthusiasm for his subject, even if the execution left something to be desired. A less trustworthy servant might have considered the opportunity offered for minor blackmail. Bunter, ever upright in his lordship's service, merely rescued the Times Literary Supplement from beneath a brandy glass, and continued his sweep of the room. Love, he mused over his bedtime cocoa, did funny things to a man. The young lady herself seemed a little less moved by the romance inherent in the situation, but under the circumstances allowances might be made. Bunter himself, only an onlooker, had found the situation less than restful. Only unusual anxiety concerning the potential effects on his lordship of an unsuccessful outcome could have caused him to be so careless as to forget to burn that last revealing sheet of paper, discovered in a pocket by its crackle as he hung up his black coat to air. The fireplace in his bedroom had been taken out when the central heating was put in, and it was hardly an urgent enough job to necessitate a return to the kitchen at this time of night. It had been a long day, and the cocoa was soporific. Still, one mustn't leave such things lying around. If one had forgotten this evening one might easily do so again in the hurry of the morning. A locked drawer was the thing, a locked box in a locked drawer. There would be plenty of time to deal with it properly another day.


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The art show must go on after tough year
ARTSTATE initiatives were not the only exhibitions taking place last week.
Lismore TAFE's Art department also went above and beyond to make sure their annual show was a success this year.
A feat indeed, considering their usual gallery for showing their art from across the various years, The Drill Hall, has been completely gutted due to the flood.
Head teacher of Creatives Industries at the Lismore TAFE's CBD Campus, Steve Giese, said it had been a "very tough year, which made the quality of work produced all the more exceptional".
He said students, some of which have considerable special needs, had come far and wide to have work completed and exhibited for the show and the standard was just as high as usual.
While there had been delays in the refurbishment of the college, he had been given assurances essential repairs would be completed in the new year. In the meantime, the department used existing classrooms to exhibit the work.
"Right now our focus is on looking to the future to support the talent that exists in the region."
One student at the campus, who wished to remain anonymous, was scathing at the length of time it has taken to get the department properly operational, as well as the way contractors stripped the buildings after the flood.
She said the canteen was still not up and running and construction fencing filled the site.
Considering the TAFE had one of the strongest ceramics departments in the country, provided training and employment opportunities, she was disgusted the facility could have been so neglected.


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I have five great kids, a ridiculously cool wife, and web marketing with some entrepreneur stuff on the brain, so you're probably going to hear about that and everything in between.
Wednesday, August 10, 2011
Basement Finishing Tips for Utah Residents
Based on the title, this might sound like helpful construction tips that would help you figure out what to do when it comes time to add on to that lowest level of your home. It is not. Rather, it is a short rant about the ridiculousness of the process. The full rant will be released accompanied with a full report card grading those who did any work in the basement at all. Now that i'm thousands of dollars poorer and tons wiser, I will share with the public what I would do next time: back up a huge cement truck to the biggest window the basement and not stop the cement until the whole thing was full. I mean it. Getting the value back from my investment is the only thing that will keep me from going postal plus. Again, the super sized rant is yet to come.
Posted by jfoutz77 at 12:28 PM
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Well, the week is now over, but the memories and emotions will remain with us for a long time (forever!?).
Here are 5 of mine …
1. Singaporeans at their best (united, helpful, appreciative, responsive, orderly … )
– the volunteers who distributed drinks, food, tissue, umbrellas, flowers (the florists who gave them out for free), the tributes and words of appreciation Singaporeans penned in fb and at tribute sites, the ushers everywhere who guided the crowd,
the orderly queues everywhere and everyday (even when the wait was 8 hours!),
the improvement of the queueing system after Day 1 (my belief that someone would do something quickly about the queuing system was right 🙂
2. The spontaneous snaking queues and how orderly it was on Day 1 even without the railings for crowd control, how long the queues were on all the other days (even in the wee hours: the girls thought of going early Friday morning but wait time was 8 hours at 3 am!), and the patience that everyone showed while in the queue (on Day 1, when SY and me joined it at 1015 am just after coming out of the Raffles Place MRT exit, it did not move at all for 30 min; then when it moved, we advanced like 5 steps …, which led to me going on a walkabout to "study" what was happening; turns out to be a bottleneck as two queues merged into one near the Cavenagh Bridge, that crosses over to Parliament House side. Everywhere I went, noticed that people waited patiently, and were in good spirits even though it was under the noon sun).
3. The best National Education one can ever get!
I think that not only students learnt so much about Singapore's recent history last week, but all Singaporeans (myself included) received a much better understanding of the events pre and post-1965. For one thing, I believed the interest shown by my girls is indicative that this would be a very special moment in the collective memory of all Singaporeans, young and old (and middle aged): how from "mudflats", we now have the modern city of today, and that it was the result of visionary leadership, hard work, courage, and commitment to make Singapore extraordinary. We must never ever take for granted that the Singapore of today is the natural order of things!
4. Standing in the pouring rain at Shenton Way on the day of the state funeral with fellow Singaporeans, and with my own family … everyone was wet even with umbrellas, but there was no grumbling heard as we braved the weather together to pay our final respects as the cortege pass the Singapore Conference Hall and AXA Tower (where we were standing on the steps) …
and finally,
5. How totally awesome LKY was as a visionary, global statesman, intellectual, mentor, father, husband, leader, … A GREAT MAN!!