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It is now the 12th of May 2018, the day of First Founding's Mayhem tournament. That also means the deadline for my half year project.
Continue reading "Project Update/Completion – Aquila Strongpoint"
A Warhammer 40k wargaming hobby blog of a Games Workshop fanboy. Painting to a table standard.
It is now the 12th of May 2018, the day of First Founding's Mayhem tournament. That also means the deadline for my half year project.
Continue reading "Project Update/Completion – Aquila Strongpoint"
First Founding Gaming club's first Warhammer 40k tournament of the year is this Saturday (12th May). Regular readers of this blog should be aware that I am in the process of painting a table's worth of scenery for this tournament, as such there has been no time to prepare a new army or even new units.
This means that once again, I'm sticking to good old Orks.
This weekend sees Games Workshop return to the Ricoh Arena in Coventry for their annual Warhammer Fest.
For the first time since they've put on Warhammer Fest, I will not be attending, as unfortunately, I am playing in a Warhammer 40k tournament on the Saturday and as much as I would like to spend my entire weekend surrounded by this awesome hobby, there is no way I'll get permission from the misses.
So that means I'm probably going to miss out on some big announcement or something, or an awesome freebie for everyone who attends…
Continue reading "Exciting Releases at Warhammer Fest 2018? Orks incoming?"
With the weather being awful recently, I have been unable to undercoat the last two scenery prices for my half year project and so I have taken the opportunity to keep my finger on the hobby pulse by building some Imperial Guard (Astra Militarum) models.
This weekend finally saw me finish both the Munitorum Armoured Containers as well as the Vengeance Weapon Battery.
Continue reading "Project Update – Munitorum Armoured Containers & Vengeance Weapon Battery"
More models finished for the gaming table project, this time it's a second Bastion and one Vengeance Weapon Battery with both weapon options.
Continue reading "Project Update – Bastion and Vengeance Weapon Battery"
So today I have subscribed to the new Warhammer Conquest magazine.
The magazine is probably not aimed at people like me who have been in the hobby for years, but I'm interested to see what the magazine contains. After all, I'm a sucker for all things Games Workshop.


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Hugepages can be allocated using the /proc/sys/vm/nr_hugepages entry, or by using the sysctlcommand.
To view the current setting using the /proc entry:
# cat /proc/sys/vm/nr_hugepages
To view the current setting using the sysctl command:
# sysctl vm.nr_hugepages
vm.nr_hugepages = 0
To set the number of huge pages using /proc entry:
# echo 5 > /proc/sys/vm/nr_hugepages
To set the number of hugepages using sysctl :
# sysctl -w vm.nr_hugepages=5
vm.nr_hugepages = 5
It may be necessary to reboot to be able to allocate all the hugepages that is needed. This is because hugepages requires large areas of contiguous physical memory. Over time, physical memory may be mapped and allocated to pages, thus the physical memory can become fragmented. If the hugepages are allocated early in the boot process, fragmentation is unlikely to have occurred.
It is recommended that the /etc/sysctl.conf file should be used to allocate hugepages at boot time. For example, to allocate 5 hugepages at boot time add the line below to the sysctl.conf file :
Before configuring Huge Pages, ensure to have read Sizing Big Pages and Huge Pages
Sizing Big Pages and Huge Pages
With the Big Pages and Huge Pages feature you specify how many physically contiguous large memory pages should be allocated and pinned in RAM for shared memory like Oracle SGA. For example, if you have three Oracle instances running on a single system with 2 GB SGA each, then at least 6 GB of large pages should be allocated. This will ensure that all three SGAs use large pages and remain in main physical memory. Furthermore, if you use ASM on the same system, then I recommend to add an additional 200MB. I've seen ASM instances creating between 70 MB and 150 MB shared memory segments. And there might be other non-Oracle processes that allocate shared memory segments as well.
It is, however, not recommended to allocate too many Big or Huge Pages. These preallocated pages can only be used for shared memory. This means that unused Big or Huge Pages won't be available for other use than for shared memory allocations even if the system runs out of memory and starts swapping. Also take note that Huge Pages are not used for the ramfs shared memory filesystem,

subset: commoncrawl


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Through model planning and conservation activities at one site, this project aims to demonstrate best practices in the conservation of in situ mosaics, and to disseminate the results in order to improve the state of conservation of archeological mosaics throughout the region. This project is a tripartite partnership of the GCI, the Institut National du Patrimoine of Tunisia and the World Monuments Fund (WMF).
Famed for its underground villas, the Roman and Byzantine-era city of Bulla Regia, located in northwest Tunisia near the Algerian border, is a rare archaeological site that contains complete, superbly preserved ancient houses decorated with wall plasters and exquisite mosaic floors.
The town of Bulla was established in approximately the fifth century BCE and reached the height of its prosperity in the second and third centuries. Most of its buildings date from this era. The site is unique among the numerous archaeological sites in Tunisia due to its below-ground architecture and because most of the mosaics adorning the houses have remained in situ, offering visitors the opportunity to view polychrome mosaics in their original context. Currently, almost four hundred mosaics have been excavated and remain exposed at the site.
This project builds on the training of technicians in Tunisia that took place over more than ten years in collaboration with the Institut National du Patrimoine (INP),and relies on those GCI-INP trained technicians currently based at the archaeological site of Bulla Regia to carry out most of the mosaic conservation work. The project demonstrates the impact of such training activities, and demonstrates a sustainable approach to conservation practice through its reliance on local resources and materials.
This project also builds on past capacity building activities in Tunisia for site managers responsible for sites with mosaics. These short courses and workshops focused on conservation and management of archaeological sites; however it became clear that a model example of conservation planning for a site with a significant number of mosaics was needed. In response to this need, the second component of the model field project is the development of a conservation and maintenance plan for the hundreds of excavated in situ mosaics at the site of Bulla Regia.


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Training Forms
The following files are available for download:
The course request form is now fillable online, when possible please fill out electronically then click the print icon. It will give the options to print a completed form, or save a blank copy to your computer. Once filled out and printed, please fax or mail in. This greatly assists with our processing.
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Phone: (208) 535-5441
Toll Free: (800) 662-0261


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RV Wood Sign 5th Wheel - Custom Carved Redwood or Cedar Personalized Camping Family Name Sign with Sign Holder Option JGWoodSigns Carved Camper Sign
Sign is 7.5" tall and 18" wide made of Redwood or Western Red Cedar material. Two lines of text. Hooks and chain included. Other graphics can be chosen, including motorhome, travel trailer and popup.
The 3 pc. 41" Round Garden Holder has a round pole and the pieces screw together with no set screw for a seamless line. It will accommodate signs up to 18" wide, with 5 or fewer add ons below it. Very sturdy! Very easy to put together and easy storage. 3pcs. Storage space needed is 12" x 20".
GRAPHIC CHOICES: http://www.woodcustomsigns.com/
FONT CHOICES: http://www.woodcustomsigns.com/font-choices.html
We have grown enough to be busy all year round. We are no longer taking special event requests for delivery less than 2 weeks out. Please order well in advance of your gift giving date. Christmas orders should be placed prior to November 20th. Place Father's Day orders prior to May 25th. We do not accept requests for Express shipping.


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Complete Day Skipper : Skippering With Confidence Right from the Start
By Tom Cunliffe
5th Edition 2016
A practical hands-on manual that covers the RYA Day Skipper syllabus in a way that reflects a skipper's growing experience at sea, beginning with yacht handling under power, moving on to boat husbandry and sailing skills, and from there to the realistic use of modern electronic navigation systems. Tom Cunliffe provides the right blend of advice, instruction, inspiration and encouragement.
Hb. 208pp 246x171mm Colour photos and diagrams throughout


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Tippawan Narintorn
Tippawan Narintorn (Tip): Assistant Director
Tip grew up in the shadow of the Sathorn Bridge but moved north to attend Chiang Mai University, which was the first time she had ever been away from home. She looked so young that during her senior year, she was assigned to spy on the new students by posing as a freshman and infiltrating their circle. Despite this vaguely totalitarian training, Tip graduated with an independent streak, determined to live free and make films. Today, she continues to stay true to these ideals, having worked on many projects, mostly in the position of assistant director. She is currently planning her next short film, which is about growing up in the shadow of the Sathorn Bridge.


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5600 Lake Resort Ter Chattanooga TN United States 37415
There is nothing a day on the lake can't cure. Lakeshore Marina is here for you to meet your boating needs. Whether it is wet or dry storage or a boat rental, we will hook you up for a great season of boating.
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Lakeshore Marina has a boat storage facility smack dab on the Tennessee River next to the Chickamauga Dam and just minutes to downtown Chattanooga. We have numerous dry inside storage racks for docking boats from fifteen feet and up and offer short term (3 month minimum - summer and winter) dry storage as well as discounted prices for annual commitments. Protect your boat from the harsh Tennessee summer sun and those cold winter days and nights (not to mention those summer rainstorms and road dirt) and add years to the life of your boat.
We offer beautiful deep water sheltered and covered wet slips at the best monthly rates around town. Our wet slips range from thirty feet (minimum) to seventy feet in length, up to twenty feet in width and up to twenty-four feet in height. Check out our rate table below. Please note that contracts are on an annual basis and an eight percent (8%) discount applies for annual contracts paid in full prior at the start of a contract term. Short term contracts are not offered for our wet slips. All wet slips have water and, except for E dock, all have electricity.
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Like our page for updates, call us at 423-870-2000, or check us out at www.lsmarina.com


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- Method
- Open Access
SNES: single nucleus exome sequencing
© Leung et al.; licensee BioMed Central. 2015
- Received: 16 November 2014
- Accepted: 18 February 2015
- Published: 25 March 2015
Single-cell genome sequencing methods are challenged by poor physical coverage and high error rates, making it difficult to distinguish real biological variants from technical artifacts. To address this problem, we developed a method called SNES that combines flow-sorting of single G1/0 or G2/M nuclei, time-limited multiple-displacement-amplification, exome capture, and next-generation sequencing to generate high coverage (96%) data from single human cells. We validated our method in a fibroblast cell line, and show low allelic dropout and false-positive error rates, resulting in high detection efficiencies for single nucleotide variants (92%) and indels (85%) in single cells.
- Coverage Depth
- Allelic Dropout
- Coverage Uniformity
- Exome Capture
- Exome Sequencing Data
Single-cell sequencing methods have the potential to provide great insight into the genomes of rare subpopulations and complex admixtures of cells, but are currently challenged by extensive technical errors and poor physical coverage data. While much progress has been made in developing single-cell RNA sequencing methods [1-4], the development of genome-wide DNA sequencing methods has proven to be more challenging [5,6], owing to the fact that single cells contain thousands of copies of each mRNA molecule, but only two copies of each chromosome. Therefore each cell provides only two template DNA molecules for whole-genome-amplification (WGA) reactions and errors that occur in the initial rounds of amplification are inherited by all subsequent molecules. In our previous work we developed the first single-cell genome sequencing method, Single-Nucleus-Sequencing (SNS), which utilized DOP-PCR to generate about 10% coverage breadth of an individual cell [7,8]. Coverage breadth is defined as the percentage of nucleotide sites in the single-cell data with ≥1X coverage depth. However, while SNS was adequate for copy number detection using large genomic intervals (54 kb), it could not detect mutations at base-pair resolution. Two subsequent methods were developed that use multiple-displacement-amplification (MDA) [9] and multiple-annealing-looping-based-amplification-cycles (MALBAC) [10] to increase coverage breadth during WGA. While pioneering, these studies increased coverage breadth at the cost of introducing high false positive and false negative error rates, due to excessive over-amplification (1:1e6) of the DNA from a single cell from 6 picograms to microgram concentrations. Consequently, it was necessary to call variants across most of the single cells to reduce the high false positive (FP) technical errors, which is equivalent to sequencing the bulk tissue en masse.
To mitigate technical errors, we recently developed a method called Nuc-Seq, which utilizes G2/M cells to perform single-cell genome sequencing [11]. While this approach was suitable for analyzing highly proliferative cells, such as cancer cells, it was not suitable for the analysis of normal cells or slowly dividing populations. To address this problem, we developed a new approach called single nucleus exome sequencing (SNES) that builds upon our previous method. SNES combines flow-sorting, time-limited isothermal multiple-displacement amplification (MDA), exome capture, and next-generation sequencing (NGS) to generate high coverage (96%) data for the accurate detection of point mutations and indels in single mammalian cells. SNES has several improvements over Nuc-Seq, including: (1) improved exome capture performance; (2) time-limited isothermal amplification; (3) enhanced MDA polymerases; (4) efficient DNA ligases; (5) quality control (QC) of WGA using qPCR panels; and (6) cost reduction by using standard reagents instead of commercial WGA kits. Importantly we show that SNES can be applied to either G1/0 or G/2 M cells, opening up new avenues of investigation into single-cell genomics studies of normal tissues and slowly proliferating cells (for example, stem cell or cancer stem cells).
Results and discussion
Experimental approach and quality control assays
To evaluate WGA efficiency we performed qPCR on each single nucleus WGA reaction using a set of 22 primer pairs that target each chromosome independently (Additional file 2: Table S1, methods). Single nuclei with 22/22 amplicons were selected for subsequent library construction and next-generation sequencing. Our data showed that G2/M cells resulted in an improvement over G1/0 cells for WGA efficiency, with 70% (14/20) single cells having the full set of chromosomes amplified in G2/M cells compared to 15% (3/20) in G1/0 cells (Figure 1c). Additional G1/0 WGA experiments and QC were subsequently performed to obtain nine SKN2 cells in total for the single-cell sequencing experiments.
Notably, performing QC analysis with qPCR on cancer cells may lead to diminished signal due to hemizygous deletions, and occasionally rare focal homozygous deletions [12,13]. Therefore users may want to consider looser filtering criteria (20/22 amplicons). However this has not been a problem in our previous work, in which we performed QC on single breast tumor cells using standard PCR and found that >20 amplicons were detected in 80 single cells [11]. Single cells that passed QC for WGA were used to construct sequencing libraries using a low-input TA cloning protocol starting with 100 ng of input material (see Methods). During library construction a unique 6 bp barcode was added to each single-cell library for sample multiplexing. We pooled four single-cell libraries together into one reaction for exome capture (TruSeq, Illumina) and next-generation paired-end sequencing on the HiSeq2000 system (Illumina) using 100 paired-end cycles. The number of cells that can be multiplexed is a function of the amount of data that is generated from the sequencing platform and the size of the exome target region.
Measuring coverage performance and uniformity
In order to assess coverage performance, we calculated coverage breadth (sites with ≥1X coverage) (Figure 2c) and coverage uniformity (evenness) (Figure 2e). Our data suggest that coverage breadth (≥1X) significantly (P = 0.0021, t-test) increased in the G2/M cells (95.94%, ± 0.005 SEM) relative to the G1/0 cells (89.60% ± 0.018 SEM) (Figure 2d). This results in the number of site with sufficient coverage depth for variant calling at 73.54% in G1/0 cells compared to 84.34% in G2/M cells. To assess coverage uniformity, we plotted the fraction of the exome covered as a function of coverage depth (Figure 2f). These plots show that the G2/M cells achieved more even coverage uniformity at sites with low coverage depth compared to the G1/0 cells. To further investigate coverage uniformity, we calculated Lorenz curves and plotted data for perfect uniformity, a genomic DNA population sample and mean data for the G1/0 and G2/M single cells, as well as data from our previous Single-Nucleus-Sequencing method (Figure 2g) [14]. These curves show a large improvement in coverage uniformity using G2/M cells compared to the G1/0 cells, and both showed vast improvements over our previous SNS approach [7]. We also calculated the on-target performance for data in the exome region of single cells, and found very high percentages (mean = 67.33%) for G1/0 and G2/M cells (Figure 2h), which is equivalent to previous reports (55% to 85%) of exome capture efficiencies using millions of cells [15].
Estimating technical error rates
Next, we calculated the FP error rate, which is caused by the infidelity of the Φ29 polymerase (error rate = 1e-7) during isothermal amplification [21]. From our data we calculated a FP error rate of 3.2e-5 for SNVs, which is equivalent to 32 errors per megabase. This FP error rate is higher than our previous estimates for whole-genome single-cell sequencing with Nuc-Seq, but can be explained by the increased isothermal WGA timeframe and additional PCR cycles required to generate sufficient DNA for exome capture and enrichment. We investigated the spectrum of the FP errors and found that 82.3% were C > T and G > A transitions (Figure 3c), showing a significant bias relative to the normal transition and transversion spectrum in the population of fibroblast cells (Figure 3d). Importantly, we found that the majority of the FP errors occurred at random sites in the genomes of single cells, with few mutations occurring at recurrent sites in two or more cells (Figure 3e, Additional file 5: Figure S3). This distribution allows the FP error rates to be mitigated by calling mutations in two (FP: 3.2e-52 = 1.02e-9) or more (FP: 3.2e-5n) single cells. Using two or more cells in variant calling is possible in most single-cell studies, which normally seek to analyze large numbers of cells.
We also investigated the distribution of allelic dropout events in the single-cell data. By comparing the allelic dropout events from both alleles, our data showed that there is a slight bias towards AB → BB dropout events, when compared to AB → AA events in both the G1/0 and G2/M cells (Additional file 6: Figure S4). We hypothesize that this bias is likely due to mismatch hybridization inefficiency of the exome capture probes to the B alleles, since they were designed for the A allele sequence (reference human genome assembly). Next we examined the distribution and recurrence of allelic dropout events by examining their frequency across multiple single cells (Additional file 7: Figure S5). Our data show that in contrast to the random distribution of FP errors that occur at different site in single cells, allelic dropout errors sometimes occurred at recurrent positions in multiple single cells (Additional file 7: Figure S5). On average we observed that 2.55 cells out of 19 single cells shared a recurrent allelic dropout event at the same nucleotide position. These regions are important to note in single-cell studies and should be filtered, since they can be misinterpreted as biological variation in SNV prevalence, when in fact they are likely to be technical errors.
Measuring detection efficiencies
We calculated the detection efficiencies, to measure the proportion of the SNVs and indels that were successfully detected in each single fibroblast cell exome. For SNVs we detected 92.37% (±0.008, SEM) of the variants in the single cells (mean = 32,369/34,982) in the G2/M cells, and 86.71% (±0.012, SEM) in the G1/0 cells (mean = 25,753/29,549) (Figure 3f). In comparison, previous studies using MALBAC [9] reported detection efficiencies of only 76% for SNVs. An alternative approach is to calculate the SNV detection efficiency at all variant sites in the reference, regardless of the coverage depth in the single-cell and population sample. This calculation results in a detection efficiency for SNVs of 60.64% for G1/0 cells and 76.22% for G2/M cells. We also calculated the detection efficiency for indels, which is 85.60% (±0.007 SEM) for G2/M cells (mean = 2,448/2,856), and 82.11% (±0.009 SEM) for G1/0 cells (mean = 1,926/2,336) (Figure 3g). To our knowledge, this is the first report showing that indels can accurately be detected in the genomes of single mammalian cells.
We report the development of a novel single-cell exome sequencing method called SNES that can achieve high coverage (96%) data from the exome of a single mammalian cell. From these data we show that we can accurately detect SNVs and indels at base-pair resolution. The technical performance in coverage improvement is due to multiple factors, including an improved Phi29 polymerase (New England Biolabs), time-limited isothermal amplification and the use of a 22-chromosome qPCR panel to eliminate cells with poor WGA performance prior to exome capture and sequencing. In contrast to our previous method [11], SNES eliminates the requirement for Tn5 transposases for library construction, which can introduce integration biases in the human genome and lead to coverage non-uniformity [22-25]. By performing time-limited isothermal MDA with an improved Phi29 polymerase (New England Biolabs) we were able to mitigate FP and FN error rates, resulting in improved detection efficiencies for SNVs and indels. Importantly, the SNES protocol eliminates commercial kits for cell isolation, WGA, and library construction, thereby reducing the cost of generating a single-cell library to approximately $30 per cell (not including the exome capture reagents and sequencing costs). This will enable a large number of cells to be analyzed and multiplexed, which is the goal of most single-cell sequencing studies.
In our study, we performed a direct comparison of data derived from G1/0 and G2/M single cells, which shows that both cells performed well for coverage uniformity and breadth. However our data show that using G2/M cells will lead to even further technical improvements in the allelic dropout rates. In the future, further technical improvements may be achieved by combining SNES with microfluidic platforms (for example, Fluidigm) which have been shown to decrease errors when nanoliter volumes are used for MDA reactions [5,26,27]. In contrast to most single cell sequencing methods to date [5,9,10,27] SNES utilizes nuclei instead of cells for analysis. Nuclei have several advantages over using cells: (1) nuclei can be stained with DAPI and gated to avoid collecting cells that are degraded, apoptotic, or replicating; 2) nuclei can be deposited more accurately to avoid sequencing multiple cells; and (3) nuclei can be isolated from archival frozen tissue samples, that have been stored for decades [5,10,20,28]. The last point is crucial for single-cell sequencing of archival tissues, because freezing ruptures the cytoplasmic membrane of most cells, but leaves the nuclear membrane intact from which nuclear suspensions can easily be prepared. However, nuclei also have several notable limitations, including potentially missing DNA from micronuclei [29] and the fact that cell surface markers cannot be used to isolate specific populations (for example, by gating during flow-sorting). Thus the choice of using nuclei vs. cells will depend largely on the specific requirements of the research project. In closing, we expect that SNES will have broad applications in many diverse fields of biology, including cancer research, microbiology, neurobiology, development, and prenatal genetic diagnosis, and will lead to vast improvements in our fundamental understanding of human diseases.
Cell line sample
SKN2 is an isogenic human fibroblast cell line that was obtained from the Cold Spring Harbor Laboratory (Dr. Michael Wigler). SKN2 was cultured using Dulbecco's Modified Eagle Medium with 10% fetal bovine serum, penicillin/streptomycin and L-glutamine.
SNES experimental protocol
Isolating single nuclei by flow-sorting
Nuclei were isolated from the fibroblasts using an NST-DAPI buffer (800 mL of NST (146 mM NaCl, 10 mM Tris base at pH 7.8, 1 mM CaCl2, 21 mM MgCl2, 0.05% BSA, 0.2% Nonidet P-40)), 200 mL of 106 mM MgCl2, 10 mg of DAPI, and 0.1% DNase-free RNase A. Cells were trypsinized and lysed using the NST-DAPI buffer. The nuclear suspension was filtered through 37-μm plastic mesh prior to flow-sorting. Single nuclei were sorted using the MoFlo Astrios Cell Sorter (Beckman) by gating cellular distributions with differences in their total genomic DNA content according to DAPI intensity. Single nuclei were sorted into individual wells in a 96-well plate. Each well was preloaded with 3.5 μL of lysis buffer (1 M DTT, 100 mM sodium phosphate, 10 mM Tris pH 8.0, and 6 M guanidine hydrochloride and PBS). After flow-sorting, the plate was centrifuged at 700 rpm for 1 min and incubated at 65°C for 10 min. After adding 1.5 μL of neutralization buffer (800 mM Trizma hydrochloride) to each well, the volume was 5 μL.
Whole-genome amplification by time-limited multiple-displacement amplification
Whole-genome amplification was performed on single flow-sorted nuclei using 10 units of Φ29 polymerase and 10× Φ29 buffer (NEB cat#M0269L), 1 mM dNTP (GE Healthcare, cat#28-4065-51), and 50 μM random hexamer (phosphorothioate modification on the two 3'-terminal nucleotide - NNNN*N*N - synthesized by Sigma Aldrich) to each well. The total 50 μL reactions were mixed by gently pipetting up and down and spinning down the reaction. Reactions were incubated at 30°C for 120 min to obtain approximately 500 ng of DNA. The polymerase was denatured subsequently at 65°C for 3 min. The amplified DNA was purified using DNA Clean & Concentrator-5 columns (Zymo D4004).
WGA quality control using qPCR chromosome panels
To evaluate the WGA amplification efficiency of each single-cell reaction we designed 22 pairs of primers (Sigma Aldrich) to target 22 loci on different chromosomes for qPCR (Additional file 2: Table S1). The primer sequences are listed below. For each qPCR reaction 5 ng of DNA was used for the KAPA Taq PCR kit (Kapa #BK1001). The qPCR reactions were run on the ABI 7500 system (Applied Biosystems) in 96-well plates. The qPCR conditions used were: 95°C for 3 min, followed by 45 cycles (95°C for 20 s, and 60°C for 30 s). Single cell WGA reactions that show positive qPCR reactions are selected for subsequent library construction and next-generation sequencing.
Construction of barcoded sequencing libraries
WGA reactions that passed QC were sonicated at 350 bp using Covaris S220. We used 100 ng of DNA to construct sequencing libraries by TA cloning using KAPA Library preparation kit (Kapa Biosystems, cat#KK8232), in accordance with the manufacturer's protocol. Libraries were quantified by real-time qPCR using Library Quantification Kit (Kapa Biosystems, cat# KK4835). We used the TruSeq Exome Enrichment Kit (Illumina cat# FC-121-1008) for exome capture in accordance with the manufacturer's protocol using eight cycles of PCR enrichment. Final concentrations were measured prior to sequencing by qPCR using the Kappa Library Quantification Kit. Libraries were sequenced using 100 cycle paired-end flow-cell lanes on the HiSeq2000 system (Illumina, Inc.) for 100 cycles. Data were processed using CASAVA 1.8.1 pipeline (Illumina, Inc) converting BCL basecall files to fastq files.
Data processing and analysis pipeline
Sequence reads in FASTQ files were aligned to the human genome (hg19) using the Bowtie 2 alignment software [30]. Samtools (0.1.16) was used to convert SAM files to compressed BAM files and sort BAM files by coordinate [31]. The Genome Analysis Toolkit (GATK v1.4-37) was used to locally realign the BAM files at intervals with indel alignment errors [17]. To eliminate PCR duplicates, we removed sequences with identical start and end coordinates using Picard software [32]. Reads with mapping quality MQ <40 were filtered from the BAM files. We used GATK Unified Genotyper to detect single nucleotide variants (SNVs). All single cells and reference samples were processed together to generate a single VCF4 file. We required a minimum base quality (mbq) of 20 for the base to be considered during variant detection. Coverage depth at a given locus of greater than 2,500 reads was down sampled to expedite analysis processing. We used the GATK variant recalibrator to filter the output at default sensitivity level. A minimum coverage depth of 6 and at least 2 reads with variant allele was used for further filtering of SNVs. SNVs in clustered regions with neighboring SNVs within 10 bp were filtered from the data to remove FPs. We then used GATK SelectVariants to separate SNVs into VCF4 files for downstream analysis. The processing pipeline is outlined in Additional file 4: Figure S2.
Calculation of coverage metrics
Reads with multiple mappings in the human genome were filtered from the BAM files. Sequence reads with unique mappings were used for calculating coverage metrics. Coverage depth and breadth were calculated using BEDTools by running genomecoverageBED using the BED file from the TruSeq capture region of 62,286,318 bp [33]. Coverage breadth is defined as the percentage of the exome with at least 1× read coverage, while coverage depth refers to the mean number of read counts across all the bases of a sequenced sample. Lorenz curves [14] were calculated to determine coverage uniformity in the single cell and population samples. Briefly, sequence reads were aligned with bowtie2 using unique mappings and PCR duplicates were removed with Picard. From the BAM files we ran samtools mpileup with the following parameters: '-A -B -d1000000000' to determine the read counts for every base in the human genome reference assembly HG18. The depth values were sorted using Unix sort with '-n' parameter and a custom perl script was used to read the sorted depth values and calculate the cumulative fraction of the genome that was covered and the cumulative fraction of reads. The curves for each cells and population samples were plotted in Matlab (Mathworks).
Calculation of technical error rates
Calculation of detection efficiencies
The detection efficiencies are calculated from the VCF4 variant files after the filtering steps have been performed. The filtered multi-VCF4 file is partitioned into separate files for SNVs and indels. For each line in the VCF file we add a binary string indicating the absence or presence of each variant in the single-cell samples or the reference population sample. For each variant site in the population sample, we identify variant sites in the single-cell samples with sufficient coverage depth (≥6X). From the binary string we determine if the variant is present/absent in each single cell relative to the population reference sample. We define a variant as being detected if the reference allele is AB and the single-cell data are either AB or BB. The mean detection efficiencies for indels and SNVs are then computed across all of the single cells.
Data access
The data from this study has been deposited into the Sequence Read Archive (SRA) under accession SRP046355.
NN is a Nadia's Gift Foundation Damon Runyon-Rachleff Innovator (DRR-25-13). This research was supported by grants to NN from NIH (R21CA174397-01) and NCI (1RO1CA169244-01). Funding for this project was also supported by the TC Hsu Foundation and the Alice-Reynolds Kleberg Foundation. This study was supported by grant from the Center for Genetics & Genomics to NN. The study was supported by the MD Anderson Moonshot Knowledge Gap grant to NN. This work was also supported by the MD Anderson Sequencing Core Facility Grant (CA016672). MLL is a CPRIT Graduate scholar (CPRIT RP140106) and is supported by the American Legion Auxiliary and Hearst Foundations. We thank Michael Wigler, James Hicks, and Linda Rodgers at the Cold Spring Harbor Laboratory for reagents and advice. We thank Louis Ramagli, Hongli Tang, Kanhav Khanna, and Erika Thompson (MD Anderson Sequencing Core Facility), and Wendy Schober (Flow Core Facility). We thank Jessica Tyler for her support.
Authors' Affiliations
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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


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Dear Friend,
My July will start with a bang with fireworks on July 4th, and finish with a flourish at the Annual Best Buddies Leadership Conference. Independence Day is one of my favorite holidays, celebrating as it does the founding of our nation and the ideals upon which it is built – life, liberty, and the pursuit of happiness.
I find it fitting that we hold our Leadership Conference the same month we celebrate our freedom. It is at the Leadership Conference that we train 1,400 student leaders from around the world in how to effectively run their Best Buddies chapter. It is thrilling to attend, knowing that they will return to their schools spreading the mission of friendship and inclusion to literally tens of thousands of people over the coming school year.
When I light my sparklers this July 4th, I will be thinking of Best Buddies and the promise of freedom from loneliness we offer to people with intellectual disabilities. Thank you for being part of our work.
All the best,
Lisa Derx
Vice President
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Mike Why 'Run My Mouth' EP Review
Pittsburgh native (and acapella mainstay) Mike Why's EP "Run My Mouth" was released with six well-arranged and diverse tracks that showcase the width and depth of Why's talents both in the studio behind the mic and the console. It is obvious Why has spent years honing his crafts in the acapella circuit and found an audience coast to coast and has since set out as a solo artist that makes a very strong statement with his first solo album, 'Run My Mouth.'
Starting with The White Stripes 2003 hit, 'Seven Nation Army' Why introduces the song as a two part harmony and within the first verse we have expanded into fully realized vocal percussion, electric guitar, point/counterpoint and an attentive call and response that finds Alex Clares runaway hit, 'Too Close' dropped in between verses. An interesting choice to bring two pieces that are harmonic cousins and have them mix well together. It is also a subtle choice as it works without having to make any rhythmic or harmonic addendums in real time and thus keeps the momentum Why establishes in the opening sixteen bars of the album. Listening to the vocal percussion on 'Seven Nation Army' the ear is never lacking variety as Why includes "live" drum fills in transitioning into different sections of the piece and also accenting key moments of the piece.
The second track is one of three original pieces Mike Why includes on 'Run My Mouth.' 'Say What Ya Say (Blah, Blah, Blah)' is a declaration of keeping focused and motivated while enduring naysayers and detractors on your own journey to the top. 'Say What Ya' say opens with a pedal point with a (vocal) horn section sitting on top that wouldn't be out of place out of a Chicago or Tower of Power record that serves as an overture to Why's anthem to self-reliance. For the most part it is an ABA form, but the midpoint of the song Why takes the hook and reharmonizes the B section before releasing us back into the A section for one last push to the end.
The Neon Trees lend 'Animal' to the third track on 'Run My Mouth.' 'Animal' combines the best of the previous tracks (horn sections, interesting vocal percussion and a deep understanding of four (sometimes five) part harmony) and showcases them in an uplifting, taut arrangement that tools his vocal percussion more an ensemble than as a substitute for a drummer. I would be curious to know how many voices Why used in the making of 'Animal,' keep an ear to the defacto bridge as Why's tenor-centric "Hush, Hush the world is quiet" takes your ear into a peaceful spot and the main melody sails over the top to bring the song to a close.
'Set Me Free' is the second original piece Why gives us in 'Run My Mouth.' As a loop artist, composer and arranger; Mike's use of reverse playback, filters and phasers pushes this song into a truer form of Electro than any other track on 'Run My Mouth.' The interludes and harmonic reworking that Why incorporates into other tracks is absent here, and I wonder what would happen if he reproached the song as a straight up Electro take. What I found effective is the use of smaller pieces of vocalization that offer new ways of exploring rhythms along the way. Instead of a true bass line, 'Set Me Free' uses a drone sitting on the bottom the entire song both during the verse and chorus. The reverse playback is used as an effect as well and not over done. The choice to use it as subtly as he does shows a deft hand in arranging that some artists lack.
'Gold Digger' finds it's way onto Mike Why's EP and rightfully so. Groove, hooks and catchy lyrics make this tune a joy to listen to, and if you have ever seen Mike Why live….a joy to perform! For me this tune is the most fully realized on 'Run My Mouth.' Mike pays tribute to both the Hip Hop and R&B worlds by using the close harmonies (Think 'Thriller') that make the transitions easy and push you over the bar line and into the next verse.
'Run My Mouth' draws to a close by revisiting 'Say What Ya Say (Blah, Blah, Blah). This time with a few reworkings and more importantly featuring a rap interlude by Billy Pilgrim. This cut of 'Say What Ya Say' like 'Gold Digger' Mike uses octaves to beefen up horn sections and also vocal pads beneath the melody. It gives the ear something to grab onto while harmonies expand and contract around them if not only to serve as extra punch both as a harmonic , but also rhythmic device.
'Run My Mouth' delivers throughout its taut six tracks and there are moments that certain clichés from the looping world could have been utilized and become, well, cliché. Mike Why gives you just enough of one thing then moves onto the next. The vocal percussion is never flashy or over the top, but it is effective both the placement and execution. A few spots lend themselves for another listen as a creative gem was starting to get culled, and then moved onto for something else, but not enough to ruin the integrity of the record.
Moving forward, I am curious to see if Mike Why employs vocal effects be it live or in post during his shows and recordings. What he delivered on this EP I have seen him very effective in translating it live, to see what happens next while be an exciting experience. On the flip side of the coin, if Mike went back into the ranks of the acapella community, how would his experiences as a solo artist effect him in that setting?
'Run My Mouth' can be picked up at Mike Why's website WhyMikeWhy.com. Mike Why can be followed on Facebook here and be kept up with in the Twitter-verse at@ MikeWhyTweets.
---Just Alliance
January. 17, 2015
Las Vegas, Nevada, USA
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Just Alliance's Blog
Thoughts, ideas and musical musings from this Silver State Loop Artist.
June 2018


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An issue film that doesn't play like one, Foster Child hangs compellingly from the shoulders of its characters, and though the influence of the Dardennes is felt, director Brillante Mendoza doesn't aim for claustrophobic effect. Like Jeffrey Jeturian's The Bet Collector, part of this year's Global Lens series, the filmmaker's docu-realist gaze absorbs a Philippine community's way of life without prejudice or judgment, roving narrow streets and capturing seemingly unrehearsed episodes of joy and panic with equal fixation. Much of the movie consists of Thelma (Cherry Pie Picache) simply getting around town and doing the only thing she knows how: being a mother. Under her foster care is a strangely silent little boy named John-John (Kier Segundo), whom she tends to just as she would her own blood, feeding him, clothing him, and in one particularly expressive scene, bathing him until she makes the mistake of going to get a towel, thus allowing him to piss on the street and run off to get dirty again. The boy's seemingly uncontrollable need to urinate is a running gag as amusing as it is touching, and as in a scene where Thelma's son helps the boy aim his dingaling into a toilet bowl, the film attests to the role of motherhood in society and the way in which behavior is ingrained at an early age. The film's goodwill is only squandered once, when Mendoza condescends to Thelma's know-how when she walks into a pimped-out shower inside a luxe hotel room, all in the interest of expressing the differences between the haves and have-nots and point out the absurdity of a shower with too many knobs accomplishing just as much—if not less—than a bucket filled with water, but the focus he places on the bounty of family ritual is not easily forgotten, not unlike the final shot in the film, which literally and figuratively looks up to Thelma and the role she fulfills in her society.
Foster Child @ New Directors/New Films
This blog entry was originally published on Slant Magazine on the date above.


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Royal Canin has announced the winners of its annual Summer of the Cat 'best display' competition, part of a multi-faceted campaign to help small animal practices develop their relationships with cat owning customers.
The Oakfield Veterinary Group based in Castle Bromwich in the West Midlands was crowned the winner after submitting a photo of their Summer of the Cat waiting room display to the pet food manufacturer. The practice wins £100 in high street vouchers in addition to the £10 voucher that every practice was awarded for entering the competition.
Royal Canin's Marianne Lomberg commented: "The display competition was just one element of this year's summer feline campaign which also featured reduced price diets, a free display package and the opportunity for cat owners to feed a rescue cat for a day, with each pet food purchase. The display element allowed our practices to give free rein to their creativity and we saw some really fantastic waiting rooms areas which were a joy to judge. We'd like to express our thanks to everyone that took part in the competition and we're confident that initiatives like this will really help to increase loyalty at participating vet practices."
In addition to Oakfield's win, there were a number of other veterinary practices commended for their entries. These included: Pype Hayes Veterinary Centre in Birmingham and Blacks Vets in Lye ; Woodcroft Veterinary Group in Cheshire and Midsummer Veterinary Group in Milton Keynes ; Better Pets in County Down ; and Allan Vets in Renfrew.


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Kowalczyk is alone but not lonely
By Mellissa Ang, photos courtesy of TAB
Clad in a simple black shirt and pants with a dark brown guitar strapped on his left shoulder for his opening number, singer-songwriter Ed Kowalczyk's I Alone gig at TAB seemed like any other live performance. But as Kowalczyk started to strum the first few chords of American alternative rock band Live's debut single Selling the Drama from their 1994 album Throwing Copper, screams and wolf-whistles echoed around the cosy TAB venue.
Within minutes, the former lead singer of Live was moving earth with his music.
Throughout the hour and a half long showcase, Kowalczyk performed nostalgic renditions of songs from Live's albums which spanned from the 90s. Songs like The Beauty of Grey, which was written by Kowalczyk at 19 years old about people of different backgrounds coming together, were reflective of the societal influences in Kowalczyk's song writing.
After parting ways with Live in 2009, Kowalczyk retained his song writing inspiration from events happening around the world with his composition of Grace, following the earthquake which devastated Haiti in 2010.
Apart from the social influences in Kowalczyk's music, a simple brown cross that hung from his neck symbolized the second source of his inspiration, his spirituality. As Kowalczyk Heaven, which he wrote when his eldest daughter Honest Sophia was born, the faith expressed through its lyrics silenced the audience.
Through the song lyrics I don't need no one to tell me about heaven. I look at my daughter, and I believe, Kowalczyk revealed the father he is to his three daughters.
Basking in all the screams, wolf whistles and huge sing-along reception he received, Kowalczyk quipped, "Well, better late than never!" when people asked him what took him so long to come and perform in Singapore.
From unique music equipment, like an old-school microphone which looked like he borrowed from John Travolta in Grease, to the makeshift cardboard boxes his drummer was playing on with neon green drum sticks, Kowalczyk went beyond any other live performance.
With his preview of an upcoming summer EP titled The Garden, we will be hearing more from Kowalczyk in the coming months. As long as Kowalczyk returns to Singapore, local Kowalczyk fans will make sure he never has to say 'I Alone' anymore.


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User Reviews for Turpentine topical
The following information is NOT intended to endorse drugs or recommend therapy. While these reviews might be helpful, they are not a substitute for the expertise, skill, knowledge and judgement of healthcare practitioners in patient care.
Reviews for Turpentine
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27 users found this comment helpful.


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Murderers, Rapists And Pedophiles Given Top-Secret Security Clearances by Defense Department
Murderers, rapists and pedophiles are just some of the people who were reportedly given top-secret security clearances by the federal government as it faces a huge bureaucratic backlog. A senior government official said Wednesday that around 100,000 people currently hold interim clearances while carrying out work for companies with Defense Department contracts, as they await more comprehensive background checks.
Related: Is Snowden responsible for leaks culture? Trump's CIA director thinks so
"This is very, very dangerous," said Daniel E. Payne, head of the Defense Security Service, which oversees the issuing of temporary clearances on behalf of the Pentagon, McClatchy D.C. reported. "I've got murderers who have access to classified information. I have rapists. I have pedophiles. I have people involved in child porn. This is the risk we are taking."
A temporary clearance requires applicantrs to merely complete a security form, a credit check and an initial FBI background check. In order to be granted a full clearance, much greater research into the applicant is required, including interviews with associates, visits to the applicant's home and deep dives into family history and overseas travel. The full process can take up to a year, Payne said.
With a current backlog of around 700,000 cases—it grew exponentially in 2015 and 2016—increasing numbers of people are being given temporary clearances for work with the Defense Department in thousands of facilities across the country. Intelligence agencies, which do not grant temporary clearances, are not affected.
The rush to get staff on board is needed in order to keep weapons development programs fully operational.
"If we did not give these individuals interim clearances, the production of these programs would shut down," Payne said. "It would have a horrific impact," he said.
Because of that, Payne said, "more than several" but "less than a dozen" people have been handed temporary security clearances who later turned out to be murderers. On one occasion, just last month, a man currently in possession of an interim clearance killed someone at a bar.
"He pulls out a gun and shoots them in the face and kills them," Payne said.
The processing time for full security clearances has grown significantly since 2013, when National Security Agency contractor Edward Snowden leaked large amounts of classified information. The following year, the company that vetted Snowden, United States Investigations Services, lost its government contract over claims it conducted improper background investigations, further exacerbating the strain on the vetting process.
Further flaws in the system were disclosed the following year after the office of Personnel Management was hacked, revealing employees' confidential information.
"This is a giant, giant problem," Andrew Hunter, a military procurement expert with the Center for Strategic and International Studies, told The Washington Post last month. "I'm told there are billions of dollars being spent for people who are on payrolls but they're not able to do the work because they're waiting for a clearance."


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Elementary Methods In Dog Collar Recommendations
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I answered for them, and soon Wendy showed up with the project vehicle. I had seen it at last year's New York International anti bark Auto Show, and it was just as I remembered. The second row and cargo areas of the crossover have been augmented with dog-friendly materials and accessories. The load floor and cargo compartment are padded and lined with easy-to-clean fabric. Spill-proof water and food dishes live in storage cubbies. Pet waste bags are dispensed from a side mount in the cargo hold, and a storage drawer for leashes and treats. There's a canine first-aid kit and a slide-away loading ramp, and a removable pet partition to keep the pups from leaping into the back seat. If you have smaller dogs, you can use a hammock that drapes from the front to rear headrests. A 10-gallon water tank with a shower hose, supplemented by a hot air blower makes vehicle-side pet clean-up possible, keeping the dirt and grime outside of the Rogue Dogue. All-in-all, it's a clever dog-friendly conversion.
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Heller After Ten Years
Ten years after the Supreme Court's landmark but controversial decision in District of Columbia v. Heller, the nature and scope of the Second Amendment right to keep and bear arms still is uncertain when it comes to a multitude of gun laws. With few exceptions, lower courts have treated Heller not as precedent to be followed, but a decision to be distinguished, narrowed, or avoided before approving almost any legislative restriction on the right to arms.
Campbell Law Review is excited to host "Heller After Ten Years" February 1–2, 2018 at the Campbell University School of Law. The symposium will examine a wide range of issues facing the lower courts since Heller, including the proper analytical framework for deciding Second Amendment cases, whether lower courts have given proper deference to Heller's recognition of the individual right to keep and bear arms for self-defense, the extent to which the Second Amendment protects certain persons, such as felons and the formerly mentally ill seeking restoration of their right to arms, and how Heller applies to restrictions on public carry and bans on "assault weapons."
1 February 2018
Heller: Past, Present, and Future
A wide-ranging discussion of the Supreme Court's 2008 decision in District of Columbia v. Heller recognizing the individual right to keep and bear arms, whether that decision has been applied properly in the lower courts, and what the Supreme Court is likely to do with the constitutional right to arms in the future.
Moderator: Joseph Blotcher
Panelists: Alan Gura, Dennis Henigan, Glen Reynolds
2 February 2018
Heller in the Lower Courts
A discussion of how federal circuit courts have applied Heller, with a focus on lower court views of Heller's holding and scope, the extent to which Heller provides a general framework for constitutional analysis in Second Amendment cases, what guidance Heller provides for resolving cases involving the right to arms in public places, the development of analytical frameworks beyond Heller, and whether lower courts have given proper deference to Heller in their Second Amendment decisions.
Moderator: Sarah Ludington
Panelists: Brannon Denning, Dennis Henigan, David Kopel, Hannah Shearer
Heller and Protected Persons
A discussion of how the Second Amendment, as interpreted by Heller, protects certain persons, including felons and formerly mentally ill persons seeking restoration of their right to arms and persons subjected to searches under the Fourth Amendment. Special attention will be given to felon dispossession cases such as Binderup v. Attorney General (Third Circuit) and Hamilton v. Pallozzi (Fourth Circuit), to the Sixth Circuit's decision in Tyler v. Hillsdale County Sheriff's Department on the restoration of firearm rights to the formerly mentally ill, and to the Fourth Circuit's en banc decision in United States v. Robinson that held that even lawfully-armed persons are per se "dangerous" and can be subjected to a Terry frisk.
Moderator: Honorable Richard Dietz
Panelists: Alan Gura, Mark Frassetto, Hannah Shearer
Lunch Panel: Justice Scalia's Use of History in Heller
A discussion of Justice Scalia's use of history in the Heller decision as a basis for recognizing the individual right to keep and bear arms for self-defense.
Panelists: Paul Finkelman, Joyce Malcolm
Heller and Public Carry Restriction
A discussion of how lower courts have applied Heller to various restrictions on the carrying of firearms in public places, with special attention given to the District of Columbia Circuit's decision in Wrenn v. District of Columbia, the Ninth Circuit's en banc decision in Peruta v. County of San Diego, and the Fourth Circuit's decision in Wollard v. Gallagher.
Moderator: Brannon Denning
Panelists: Joseph Blocher, Jonathan Lowy, George Mocsary, Glenn Reynolds
Heller and "Assault Weapons"
A discussion of how Heller has been applied to "assault weapon" bans, with special attention given to the Fourth Circuit's en banc decision in Kolbe v. Hogan, which held that the popular AR-15 rifle and other "assault weapons" are not protected arms under the Second Amendment.
Moderator: E. Gregory Wallace
Panelists: David Kopel, Jonathan Lowy, Allen Rostron


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Weather changes... heaven or our own doing?
Weather... an issue that seems to be birthing more thoughts and more religious conversations each day. The "end times" are being blamed for the increase in Tornados, Hurricanes, Tsunamis and any other horrendous weather we might be seeing today. There is no doubt, an increase in our "nasty" weather. Even here in Iowa, we don't see rain anymore. We see storms that bring with them such wind and lightening that it is frightening. We spend more time in our "basement", staying safe from Mother Nature's fury.
I am not one to push away the thoughts of the "end times." I have studied the Bible for most of my life. I understand that the end times are to bring fierce weather to the Earth. But am I ready to put blame on God and the heavens bringing upon us the "end times?" Many believe that All weather comes from the heavens. I am not here to discard that thought or to shut out God and his power. I am here to offer another thought to carry with you. Something to ponder and perhaps give a little reasoning to the weather change.
When the weather began taking on a new ferocity, I too tried to reason why. I was watching the war in Iraq and the devistation being done there by bombs, missles and any other type of explosive weapon used. I watched as the smoke rose into the sky and spread across ocean. I thought about the sulphur that was being deposited into the clouds and sea water. My mind wandered back in time.
Growing up in Florida, droughts were a way of life. In order to combat the rainless skies, airplanes would be sent up to "seed" the clouds. Seeding consisted of flying into sky and disbursing silver iodide and dry ice into the air. The pulses would encourage atmospheric sulfur dioxide and nitrogen dioxide to form particles that would seed the atmospere. the results were rain coming to a much needed region. It is a process that is still used today.
While thinking about this, I watched the black clouds rising from the burning oil wells and the fallout being brought on by the explosion of bombs. I could not help but wonder if the inrease in atomspheric activity was going to have a impact on our eco-system and our weather. Only a theory, I remembered the "butterfly effect." This is the knowing that when a tiny wind blows through an area, such as the Rain Forest, that wind will cause another to blow. The wind, caused by something so small as a "butterfly" spreading it's wings and taking flight, is enough to cause the wind to move. Another on another and you have winds blowing across the forest.
In line with this theory, I believe that the elements being mixed in our atomosphere today, due to pollution from cars and industrial plants and yes, the wars we are fighting, is a major cause of the weather we are seeiing every-where. The increase in storms and tsunamis is a direct result of the pollutants we are covering the Earth's skies with, today. We, as the inhabitants of this planet, are causing our own destructive weather. Every element we send up into the sky has an effect on our weather. We know, with total certainty that pollutants cause damage to our air and the sky above us. We know that the more we send up, the more changes we will see. This is not God, seeking to harm us or destroy the Earth and all of it's inhabitants. This is Us! We are doing the damage that is causing such horrible weather to plague us. We, as a people, have the ability to stop some of this from happening. I believe that the weather and many other changes are going to increase unless we do something to stop the constant polluting of our atmosphere.
The "end times" reigning down upon us? Probably not! Our own need to add more "garbage" to the air we breath, causing such terrible weather to rain down on us? it is something to think about.
Something to think about
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As Atlanta Matchmakers we know that whether it's because you lack the experience, or have history of a rotten ex (or five!), most of us tend to over analyze the act of dating. With one study's statistics stating that a whopping 62% of you think that other people have too high of expectations on a first date, it can overwhelm and intimidate even the most confident person. Here are a few pointers from… Read more
Euro 2016 is in full swing, and if you're a soccer fan you've been enjoying watching the tournament that's been taking place in France. It got me to thinking about the trip Leisha and I took to Paris two summers ago. In celebration of a fun tournament and as a bit of a rememberance of our time in France, we're revisiting a post we wrote two summers ago that looked at some of the top French restaurants… Read more
Ladies when it comes to looking for love you can follow your heart and you can follow your head. As your Atlanta Matchmaking experts we'd like to share some tips to consider while dating in Atlanta in your search for Mr. Right? Avoid these common dating mistakes and you are sure to find him! Waiting by the Phone Keep yourself busy! Take classes, join groups, hang out with your friends, ENJOY LIFE!… Read more
Let's face it; we're getting into the "dog days of summer" with no fun-filled holidays in sight. Why not take advantage of a local place with lots to offer for Atlanta dating? Atlanta Dating Spot of the Week Our pick of the week is the Piedmont Park Conservancy, located about 10 minutes North of downtown Atlanta, it's a little slice of paradise nestled in between big city sky scrapers. The park has… Read more
Looking for a new and exciting way to do something new and interactive on your next date? Get out of your "dinner and a movie" rut and plan a get physical date. Here are four activities that most of us usually don't think about when we plan to exercise that would also make a really fun date if done together. These ideas are all local and ideal for an upcoming weekend. Do the Silver Comet Trail Get… Read more
Remember the old pick-up line, "What's your sign?" Well, for those who are astrologers, and earn their living reading birth charts and forecasts, it's no line. "Your Sun sign tells so much about you---and those with whom you are compatible. However, there is more to your birth chart than your Sun sign, which is why I need the exact time you were born, as well as the month, day, year, and city",… Read more
This week's pick has to be one of my favorite places in the city! With a canopy walk which makes up a magical forest of hundreds of plant species, and a beautiful courtyard that opens up to various art sculptures and flowers, this is the perfect place to go on a date, day or night. The Atlanta Botanical Gardens has detailed plant exhibits based on the season, but each one is intricately planned so… Read more
A wise man once said: "Eat tapas!" Lucky for you, we've found just the place for feisty fun, and delicious delicacies that would put any dinner-and-a-movie to shame. Loca Luna is a Latin restaurant, bar, and dance club hidden in the northeast of Atlanta. This hotspot not only boasts some of the most famous tapas and exotic drink specials in the city, they add amazing Latin flavor by hosting Salsa,… Read more
We meet a lot of people who are disenchanted with online dating. Online Dating statistics show that 40% of on-line candidates are married, cheating on their spouses, misrepresenting important facts about themselves or just game players. Even prisoners can have access to the internet. Matchmaking, though is different. We get to know each of our client's personally in a face-to-face meeting. We form… Read more
This is one of our favorite Date Spots. It combines great food, a unique Southern atmosphere, and music rolled into a quaint restaurant in Vinings, the Old Vinings Inn. The Old Vinings Inn is set in a 150 year old former Inn and Tavern located on Paces Mill Road just inside the Perimiter. There are several rooms available dining from the main dining room, the Bear room, the Wine room, the Garden… Read more


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NBA's "Train the Trainers" program concluded in Chennai
Jr. NBA WNBA Train the Trainers program concluded in Chennai on 24th march 2011.
NBA hosted this basketball clinic. In partnership with BFI, the "Train the Trainers" program was a free opportunity for coaches and fitness trainers from Chennai to learn the coaching techniques from Martin Conlon.
Martin Conlon (born January 19, 1968 in the Bronx, New York) is a former professional basketball player whose career in the National Basketball Association lasted from 1992 through 2000.
The two day program concentrated on basic fundamental skills, including ball handling and dribbling, passing, shooting, one-on-one offensive moves, footwork and rebounding.
On the first day of the program, Marty taught the basic drills and demonstrated the skills challenge. He worked on the "strength and conditioning for basketball" on the day2.
The clinic concluded with a question-and-answer period with Marty, where he answered all the questions raised by the participants.
"I was really excited about Chennai", Joel said on Friday. "The weather is great, the hospitality was excellent and the participants response was good."
Marty provided words of encouragement to the participants, reminding them of the hard work and constant practice it takes to develop into a better coach.


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Buying a home is an exciting and complex adventure. It can also be a very time-consuming and costly one if you're not familiar with all aspects of the process, and don't have all the best information and resources at hand.
I work especially well with those of you looking for something a bit out of the ordinary, older, funky, or with needs for special lighting, settings, etc. With a creative background and vision for improvements as well as a keen sense of the link between location and investment, I have helped buyers realize their dream of home ownership throughout the years.
Call me at 914.438.1999 to discuss how I can help you achieve your goals
Preview our Exclusive Buyer Handbook
Suzanne L Stevens
Cell: 914.438.1999


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Grundfos Dosing Pumps
The Grundfos DMX range has proven its worth in dosing applications worldwide. With their robust diaphragm-based design and their high-quality motors DMX pumps require minimum maintenance and are best choice for many dosing applications. The DMX range is highly versatile: it covers a wide flow range and offers a variety of dosing head sizes, materials and accessories.
Grundfos offers a wide range of dosing pumps representing state-of-the-art technology.
Digital Dosing solutions (SMART Digital, DME, DDI) set new standards in liquid chemical handling and accuracy. The operator can easily install and set up the pump to discharge exactly the quantity of dosing liquid required for the process in a unique drive- and flow-control management concept. The setting of the pump is displayed in ml/h, l/h or gph, and the operation mode is easily identified by means of icons.
Measuring amplifiers and controllers
The Conex® DIA (Dosing Instrumentation Advanced) series is designed for users without previous knowledge. A simple plain-text menu allows you to complete your tasks quickly and easily without wasting any time on learning and deciphering codes. The units speak no less than nine languages, allowing virtually anyone to navigate the self-explanatory menus. Conex® units monitor themselves, ensuring high water quality at all times.
OCD-162 Oxiperm Pro :
An ideal means of ensuring the cleanliness of drinking water is to use chlorine dioxide as a disinfectant. Chlorine dioxide is highly effective against all types of germs and has a long residual time in the system, meaning that it disinfects even without extracting water. The big advantage of chlorine dioxide over other disinfectants is its effectiveness against biofilms. The chlorine dioxide production system, Oxiperm Pro OCD-162, is the perfect solution for fighting Legionella and other germs in drinking water.
Vaccuperm VGA and VGB :
The gas dosing systems Vaccuperm VGB and VGA are designed for the safe and accurate dosing of gaseous chlorine. This series is available in measuring ranges for up to 10,000 g/h chlorine.
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text: Home > Hong Kong > Radio 2 RTKH Radio 2 RTKH Webmaster Last updated : March 2, 2011 Hong Kong Tweet Pin It Radio 2 RTKH :: www.rthk.org.hk Tweet Pin It Tags:Hong Kong Leave a Reply Cancel reply Your email address will not be published. Required fields are marked *Name* Email* Website Notify me of follow-up comments by email. Notify me of new posts by email.


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It is with great sadness that we report the passing of Rebbetzin Chaya Epstein a"h, wife of Rav Aharon Zelik Epstein zt"l, rosh yeshiva of Yeshiva Shaar HaTorah-Grodno of Queens.
The Rebbetzin was a remarkable woman who not only stood at the side of her illustrious husband throughout his life but also served as the aim hayeshiva of Shaar Hatorah, worrying about the talmidim and opening her home to one and all.
Rav Zelik learned under Rav Shabsi Yogel zt"l, rosh yeshiva of Yeshivas Slonim, and later the Mirrer Yeshiva in Poland, where he became close to Rav Yeruchem Levovitz zt"l and Rav Leizer Yudel Finkel zt"l. During World War II, the Mir Yeshiva fled from one place to another in order to escape the bloodthirsty Nazis: Vilna, Keidan and Krakinova, some to three small towns -Ramigola, Shat and Krak. Then the yeshiva left Europe and arrived at Kobe, Japan, and finally Shanghai, China, where it remained until 1947.
Arriving in Toronto with 26 other Mir Yeshiva talmidim, Rav Zelik continued his intensive studies. There he met Rav Yaakov Kamenetsky zt"l, then rov in Toronto and later rosh yeshiva of Torah Vodaas, and an instant bond followed. When Rav Zelik married Rebbetzin Chaya, their wedding took place at the home of Rav Kamenetsky.
Rebbetzin Chaya was a daughter of Rav Moshe Mordechai Shkop zt"l (1882-1975), rosh yeshiva and rov of Grodno, who escaped to America in 1941. Rav Moshe Mordechai was a son of Rav Shimon Yehuda Shkop zt"l (1860-1940), rosh yeshiva in Telz (1884), rov of Moltsh (1903), Bransky (1907) and Grodna (1920), and author of Shaarei Yosher, and rosh yeshiva at Yeshiva University from 1928 to 1929.
In 1948, Rav Kamenetsky became a rosh yeshiva at Yeshiva Torah Vodaas, and in 1950 he recruited Rav Zelik to become a rosh yeshiva there. In 1965, together with Rav Nesanel Quinn zt"l, who served as menahel of Yeshiva Torah Vodaas for more than 70 years, Rav Zelik Epstein established Camp Ohr Shraga, the Torah learning summer camp in Greenfield Park in the Catskills.
In 1974, Rav Kalman Epstein, son of Rav Zelik and the Rebbetzin, established Yeshiva Shaar HaTorah-Grodna in Kew Gardens, Queens.
The home of the Epsteins was always open and he welcomed all who came to their doorstep seeking advice, regardless of whether it was a rov of a kehillah, a rosh yeshiva, someone with sholom bayis problems, a person seeking medical advice, someone with career choice quandaries, or even one seeking the simple translation of words of Chumash or siddur.
Rav Zelik and the Rebbetzin received everyone with a warm engaging smile and devoted his full attention, regardless if it was a summer afternoon or Erev Yom Kippur. Students and strangers were equally ushered into the home.
In one instance, some years ago, a middle-aged rosh yeshivah with a large family became terminally ill. Throughout the illness, the Rebbetzin and Rav Zelik became virtually surrogate parents, visiting, counseling and offering strength and encouragement. When the sad day came, the rebbetzin and the rosh yeshiva were with the bereaved family for hours at a time. To anyone who knew the Rebbetzin, this was not at all surprising. In their quiet way, the Epsteins could not conceive of acting differently.
The Rebbetzin is survived by her son, Rav Kalman, rosh yeshiva of Yeshiva Shaar Hatorah; her daughter, Mrs. Elka Mordechovitch; and grandchildren, and great-grandchildren, all who follow in the path paved by their zaide and bubbe.
The levaya will take place this Motzoei Shabbos at 7:30 p.m. at Yeshiva Shaar Hatorah-Grodno, located at 117-06 84 Avenue in Kew Gardens, Queens.
When Rav Zelik passed away three years ago, it was felt that with him, the last vestiges of Grodno and Mir – greatness in Torah, middos, yiras Shomayim, and an ability to inspire and teach and guide and inspire with unpretentious greatness – was gone. With the passing of the Rebbetzin, the remaining connection has completely been taken from us.
Yehi zichro boruch.
{Noam Amdurski-Matzav.com Newscenter}


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Kathleen AndersonCollections Specialist
Kathleen has over 30 years of experience working with accounts receivable along with an extensive customer service background. Originally from Cottonwood, ID, she has lived her entire adult life in Spokane. She lives with her husband and has raised two grown children. In her spare time, Kathleen volunteers with Spokane County's CASA program.


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LAHORE – A laptop distribution ceremony was held at National College of Arts on Saturday.
On the occasion, Special Assistant to CM Punjab on Education Zaeem Qadri, advisor to CM Zakia Shahnawaz, Shaista Pervaiz Malik, Uzma Bukhari, Principal National College of Arts Dr Shabnam Khan and teachers were present.
Addressing the ceremony, Zaeem Qadri said said that PML-N government had made record investment in education sector for the first time in the history of country. He said that Punjab government had made unprecedented development work in education, health, transport and other sectors.
Later, Zakia Shahnawaz inaugurated the Art Gallery at National College of Arts. She urged upon that students to play their due role for giving a new recognition to Pakistan at international level by using their talent in the fields of fashion, jewellery, designing and other sectors. On the occasion, laptops were distributed among 238 students.


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0156 Escape from the Amazon (Part 2)2010 年 10 月 28 日 木曜日
Four men; Carl who was the guide in this group, Marcus, Kevin and Yossi were exploring in the jungle of Amazon. But they got lost in the jungle.
They discussed what to do next when their raft arrived at the shore. However, they had no cooperation and no friendship. In addition, Marcus injured his leg in this adventure. His injury was serious.
Some of them wanted to go to the canyon. But if they continued this adventure, the problem was that they had to leave behind Marcus. They had to decide that they continue this adventure or give up.
But if I were a member of them, I would like to search for the way that all the members can cooperate and continue this adventure.


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Migrant labour is being misused to drive down wages paid in the telecommunications sector, claims one industry participant.
The telecommunications services industry may soon become totally reliant on imported labour, services company TransWorks claims in a submission (pdf) to Ministry of Economic Development.
Managing director Mike Paltridge says the industry is dominated by two companies, Downers Engineering and Transfield Services, both of which are overseas owned.
"If either decided to exit New Zealand, there is no one big enough left to pick it up easily," he told Computerworld. "The smart thing is to rebuild this industry. The government should open up some work to third parties and not keep it locked up."
The submission notes that both Downers and Transfield are accredited employers for the purposes of sourcing staff directly from overseas. From January 2003 to September 2007, 814 work applications for telecommunication technicians were approved.
Paltridge estimates there are around 1000 employees in the sector, with Downers and Transfield each employing around 300.
Comparative figures sourced from the national employers 2007 wage and salary survey show telecommunications technicians are paid far less than their counterparts in other areas of the industry. An electronics technician earned $23.76 an hour, and a computer systems technician $24.56 an hour; the typical wage rates for telecommunications technicians at service companies ranged from $16 an hour to $22 an hour.
Specifically, Telecom technicians have not had a pay increase since their last collective agreement was negotiated in 1998.
Last July, the Engineering, Printing and Manufacturing Union's ElectroComms industry council called for the government to crack down on what it said was the widespread misuse of migrant labour to exploit local and immigrant lines workers.
The council said companies across the industry were keeping wages and conditions for local and migrant lines workers in check by misusing systems designed to cover skills shortages.
Paltridge says in his submission that Downers and Transfield had rejected suggestions from Telecom project managers that they subcontract work to other, smaller companies. Instead, they had approached the Immigration Department requesting dispensation to bring foreign nationals into New Zealand as technical staff.
"We are dominated by the two," he told Computerworld. "Between them and Telecom driving down costs, it's having a dramatic affect on the industry."
In TransWorks submission, Paltridge says the separation offers an opportunity to revitalise and re-invest in the service industry.
Neither Downers nor Transfield responded to calls from Computerworld.


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Over 30 years of experience in protecting business owners in the Volusia and surrounding counties. As a family business ourselves, we understand the needs and challenges of protecting a business that is your main source of income but not breaking the bank. We will do our best to find you the best price for protecting your assets.
Please contact our agency if you have any questions, or if you would like a proposal please fill out forms below.
Typical business package policies include the following coverage:
Liability Insurance Request Form
Property Coverage
- Buildings
- Contents
- Equipment
Commercial Auto | School Auto Insurance Request Form
- Van
- Buses
- Work Trucks
Please click on the icon for a Commercial Auto Proposal. You will also need to Click > Driver Information and FAX or EMAIL this form to us.
Workers Compensation Insurance Request Form
Construction Industry: An employer in the construction industry who employs one or more part or full-time employees must obtain workers' compensation coverage
Non-Construction Industry: An employer in the non-construction industry, who employs four or more part or full-time employees, must obtain workers' compensation coverage.
Read More on Florida Workers Comp Law > HERE <
Please click on Employee Information Form to fill out and attach to form at the end of form.
Child Care Centers & Schools Insurance Request Form
We also offer policies specifically tailored for child care centers and schools. Please click on the icons below to receive a FREE proposal.
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- Counseling Sexual
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- Liability Directors &Officers
- Corporal Punishment
Workers Comp Safety Manuel
Already have insurance? We can review your existing policies to see if we can better serve your needs. Fill out this form and one of our representatives will contact you to offer you a no-obligation review.
Or you may also be interested in the free information guides we have concerning personal and commercial insurance
Contact Form:
If you have specific questions feel free to contact us via email,
or you can call us @ 386.574.3030 or toll free: 800.329.3031


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Get Plugged In: Rocksmith will include a unique quarter-inch to USB cable that is the first of its kind, and will allow users to plug any real guitar with a quarter-inch jack directly into their Xbox 360®, PlayStation 3®, or PC. Developed exclusively for Rocksmith, this guitar cable is 11.25' and turns the guitar's signal from analog to digital, allowing it to be recognised and played through video game consoles for the first time.
There's always a way to develop your guitar skills and overcome your difficulties: Having troubles with the solo of your favourite song? The riff-repeater option is your saviour. It will isolate the song part of your choice, and let you repeat it over and over … until you eventually ace it! Want to improve your finger dexterity? Learn chords? Improve scales? For all of these and more, there's a specific mini-game that will help you get it in Guitarcade. Rocksmith will turn your guitar into a controller, for instance, while shooting ducks in the Ducks mini-game you'll actually be learning how to shift from fret to fret without even realising!
Turn your TV or computer into an amplifier: No need for an amplifier anymore, with Rocksmith your TV or your computer IS your amplifier and will let you try and play with loads of in-game pedals, amplifiers and cabinets. String pedals together with nearly infinite combinations and tweak your sound until you're satisfied. Whether it's a crunchy distortion, a vintage tremolo or a psychedelic reverb, we have it covered to make sure you can achieve the sound you're looking for.
Best Coast - When I'm with You
Blur - Song 2
Cream - Sunshine of Your Love
Dan Auerbach - Want Some More
David Bowie - Rebel Rebel
Eric Clapton - Run Back to Your Side
Franz Ferdinand - Take Me Out
Incubus - I Miss You
Interpol - Slow Hands
Jarvis Cocker - Angela
Jenny O.Well - Ok Honey
Kings of Leon - Use Somebody
Lenny Kravitz - Are You Gonna Go My Way
Little Barrie - Surf Hell
Lynyrd Skynyrd - Sweet Home Alabama
Muse - Plug In Baby
Muse - Unnatural Selection
Nirvana - Breed
Nirvana - In Bloom
Pixies - Where Is My Mind?
Queens of the Stone Age - Go with the Flow
Radiohead - High and Dry
Rapscallions - California Brain
Red Fang - Number Thirteen
Sigur Ros - Gobbledigook
Silversun Pickups - Panic Switch
Soundgarden - Outshined
Spoon - Me and the Bean
Stone Temple Pilots - Vasoline
Stone Temple Pilots - Between the Lines
Taddy Porter - Mean Bitch
The Animals - House of the Rising Sun
The Black Keys - I Got Mine
The Black Keys - Next Girl
The Boxer Rebellion - Step Out of the Car
The Cribs - We Share the Same Skies
The Cure - Boys Don't Cry
The Dead Weather - I Can't Hear You
The Horrors - Do You Remember
The Rolling Stones - (I Can't Get No) Satisfaction
The Rolling Stones - Play with Fire
The Rolling Stones - The Spider and the Fly
The Strokes - Under Cover of Darkness
The White Stripes - Icky Thump
Titus Andronicus - A More Perfect Union
Tom Petty and the Heartbreakers - Good Enough
Velvet Revolver - Slither
White Denim - Burnished
Yellow Moon Band - Chimney