text:
Hi everyone,
Today I wanted to share a great insight I got through the Coaching for Executives training program which I undertook recently. I am taking it for two reasons: one – to become highly trained in the most pressing issues executives and managers have in the complicated, multicultural and very dynamic world today; and second – to be able to give valuable insight and possibly internal or external coaching services to those who strive for excellence, results and growth.
These goals would be possible through thorough application of highest level of coaching knowledge and techniques developed and honed by our mentor Andrew Neitlich, who is Harvard Business School graduate and International Coaching Federation's ACTP training program leader, having more than 20 years of experience consulting and later coaching high shot executives in different industries.
Anyway, so I was practicing with another coach-in-training, simulating a complicated situation, where a software group manager, having 3 teams and around 20 people under him, needs to raise the effectiveness of his people, making them more active, pro-active and enthusiastic so that development is faster while maintaining high quality of deliverables.
Final results of this coaching should be:
1. all tasks done in estimated time or shorter
2. task estimations become shorter and more concise
3. people initiate needed changes and improvements
4. people take more responsibility in the quantity and quality of their work
Sounds familiar? 🙂
So, after analyzing the team leaders and other group members behavior, I reached conclusion, that I would need to get better cooperation from the team leaders. And point is: some of them ARE STRONG HEADED! Which clearly causes other people in the team and the group to look up to them instead of cooperating with the required improvements and changes.
The reasons why it is so are clear – the guys are more knowledgeable in the existing solution, more experienced with the different problems the software faced and has successfully resolved, resulting in many hacks and workarounds which only developers know, and so they are unwilling to receive lead from a person without all of this knowledge.
So what I found out as a good approach partially because it is based on my current knowledge on human nature and how the mind works: people must be as right as possible in any situation and this rightness is dictated by what THEY know and what THEY believe is true.
So the best way I could see to get the desired cooperation would be in fact to cause such a person to bring forth his OWN solution to the business and company needs, so it would be HIS and not someone else's.
That way I would only state what is needed from the team and the reasons behind it and then ask very respectably NOT for the opinion, no! For the solution and preferably 2-3 of them with the full reasoning behind them, so the I-KNOW-ALL person himself would fight for the right solution which seems to solve the business needs, such as making work more efficient and productive for the entire team.
Plus, if a solution is accepted, which was born by such a lead player of the group – it has much more chances to become reality, than if it was planted fully from an outsider! Because if a manager or such an outsider "said so", it would certainly result in team and group automatic resistance with mechanical movement toward the stated goals without heart, enthusiasm or attention to the details or quality.
When people believe in what they do and are enthusiastic, then they move faster, they think clearer and they tend to take more responsibility and act. While totally on the contrary, when their opinion, knowledge and experience are disregarded and not fully consulted, they would be apathetic, slow and mentally dispersed.
Hope this article would help to smooth out some conflicts which are many times a sad reality in R&Ds, groups and teams all over.
To your success,
Alexander Stern
Software Architect, Consultant and Coach
Effective R&D Evangelist


text:
If you're a manufacturer or a business owner looking to grow, consider adding exporting to your distribution mix.
Why? Because roughly 95 percent of the world's population and 70 percent of the world's wealth is overseas. The fact that most small American manufacturing companies limit themselves to domestic distribution constitute a major missed opportunity.
Exporting brings many benefits. First and foremost is the potential for substantial growth in sales, revenue and profits. Adding export capacity diversifies sources of income, reducing dependence on the U.S. market and its ups and downs. It can blunt the impact of seasonality, and it can absorb excess inventory, eliminating the need to sell at a discount. In addition, it can increase productivity, improve competitiveness and spur innovation.
But exporting isn't for everyone. It adds complexity, and it's not risk-free. Deciding whether this is a viable growth avenue for your company requires a two-part analysis.
The first involves assessing how export-ready your company is:
- Can you allocate funds to research foreign markets?
- Can you increase production with the equipment you have? What about other production elements? Labor, warehousing and access to parts and materials?
- How easy would it be for you to obtain export financing?
- Trade has its intricacies- shipping, foreign exchange, communication with foreign buyers. Are you willing to learn what it takes?
The second part of the analysis focuses on externalities:
- Do you have a product or service that is in demand overseas? Would it have or technology advantages over products already available?
- How different is the business culture or political system in your target market?
- What about distribution networks? Labeling and packaging requirements? Tariffs? What other obstacles exist?
- All told are the challenges acceptable or overwhelming?
Becoming an exporter may seem daunting. But resources are available to companies interested in finding a way.
Yes, exporting can make an entrepreneur's life more complicated. But help is available and if you investigate this option, you may find that the rewards are potentially great.
By Robert Saikali, Vice President of International Banking & Trade Finance for City National Bank.

subset: commoncrawl


text:
Madison - St. Clair Record
The Newsletter Bringing the Legal System to Light
No Thanks
Will be used in accordance with our privacy policy.
Monday, March 30, 2020
By The Madison County Record |
Apr 1, 2006
Washington Street Grille 318 E. Washington St Belleville, IL Serving lunch and dinner daily
10th & West Main, Belleville
Golden Dragon Chinese Cusine
128 West Main, Belleville, IL
Dine in or carry out
By The Madison County Record |
Apr 2, 2006
Way back when The Record was a fledgling publication trying to earn respect around the courthouse, Chief Judge Edward Ferguson was seen picking up copies around the office and tossing them in the garbage.
Madison County's new chief judge may have a plaintiff's pedigree. But she's talking like a tort reformer.
By Steve Gonzalez |
Apr 2, 2006
84 Lumber
By Ann Knef |
Apr 3, 2006
Diane L. Truttman claims a van's airbags and seatbelts were defective in a lawsuit filed against General Motors in St. Clair County on March 28.
Volkswagen of America has been targeted in a St. Clair County class action lawsuit by a group that claims the automaker unfairly changed maintenance specifications which more than doubled their oil change costs.
By Steve Gonzalez |
Apr 3, 2006
Three St. Clair County residents are seeking to be representatives in a class action case filed against Illinois Power Company in St. Clair Circuit Court March 13.
Barnes Jewish Hospital in St. Louis Ethel Wright filed a wrongful death suit against Barnes Jewish Hospital, Washington University and Saiyed Aon Ali Mohib, M.D., alleging the defendants failed to exercise reasonable care customarily provided in the health care industry which caused her husband's death.
Debra Thompson v. Michael Mueller
By Steve Gonzalez |
Apr 4, 2006
Auffenberg's St. Clair Auto Mall An O'Fallon woman filed suit against Auffenberg St. Clair Auto Mall claiming that seven days after she purchased a used vehicle, the brakes failed causing her to sustain serious injuries.
George Huskey filed a personal injury suit against Union Electric Company and McCarthy Building Companies in St. Clair County Circuit Court March 20, alleging he was injured while working at UE's Venice plant on June 6, 2005.
World Market in Shiloh Christina Scott claims headboards or footboards fell onto her left foot in a lawsuit filed against World Market in Shiloh in St. Clair County on March 17.
Jerry Moeser filed a medical malpractice suit against Donald Serot, M.D. and Belleville Memorial Hospital in St. Clair County Circuit Court March 30, seeking damages in excess of $250,000.
By Carrie Gonzalez |
Apr 4, 2006
9 a.m.
By Ann Knef |
Apr 4, 2006
The Illinois Supreme Court is requiring that all state judges take 30 hours of continuing legal education courses over a two-year period, addressing such issues as judicial conduct, ethics and professionalism.
By The Madison County Record |
Apr 4, 2006
Powered by generators, Community First Bank in Fairview Heights was one of a few businesses able to service customers Monday in the storm-ravaged community.
The Illinois Supreme Court refused to hear the appeal of defendants in a $1 million Madison County jury verdict -- the only welding rod trial successfully litigated in the United States.


text:
November 6, 2007
Toronto, Ontario—(BUSINESS WIRE)—Eyelit Inc.,
a manufacturing software provider for visibility,
control, and coordination of manufacturing operations
for the aerospace & defense, electronics, life sciences,
semiconductor and solar industries, announced today
that Infotonics Technology Center (ITC) in upstate
New York has selected Eyelit's Enterprise Manufacturing
Execution (MES) and Quality Management Software
Suite for improving overall process control in its
state-of-the-art, collaborative manufacturing facility.
By catering to numerous corporations and universities,
in collaboration with the State of New York, ITC
has established itself as the premier manufacturer
in the development and commercialization of innovative
MEMS (micro-electro-mechanical systems) based photonic
and microsystems technologies. Control and stability
of newly defined processes and processing equipment
are critical to the success of any new product development.
Eyelit's Manufacturing Execution System and Asset
Management software packages will provide Infotonics
with the informational data centers necessary to
define, analyze, and control the processes and products
their customers are defining.
"Eyelit's solution caters to ITC's needs on two
levels," said Crispin Panyard, Vice President of
Operations for Eyelit. "First, the Center needed
an easy-to-deploy, user-friendly software package
that allows everyday users to rapidly define and
change processes on their own. Second, it required
an off-the-shelf package that replaces ITC's existing
traceability system as well as providing additional
benefits to support the company's rapid growth."
"Eyelit's solution was attractive, as it seems to
have become the de facto standard among MEMS companies,"
said Glenn Fricano, Vice President of Marketing and
Sales for ITC. "It is very important in the manufacturing
of microsystems to be able to connect the front-end
(wafer fab) process seamlessly with the back-end
assembly. Eyelit's MES provides the capability, which
allows us to understand how certain variables may
affect the results of our processes. This knowledge
helps empower our customers to bring their products
to market faster."
About Eyelit Inc. (www.eyelit.com)
Eyelit is the leader in Manufacturing Execution and Business Process Management (MES and BPM) solutions for visibility, control, and coordination of manufacturing operations for the aerospace & defense, electronics, semiconductor and solar industries. Eyelit uniquely delivers a broad set of manufacturing solutions, including Asset Management, Factory Integration (Automation), Manufacturing Execution (MES), Supply Chain execution, Quality management (CAPA/OCAP), and Business Process Management, that enable our customers to rapidly and cost-effectively optimize production and company processes. With exceptional customer service, Eyelit has time and again proven superior innovative technology can deliver quicker time to value. More than 40 leading companies, including Analog Devices, austriamicrosystems, Freescale Semiconductor, Fujifilm, Innovative Micro Technology (IMT), Tower Semiconductor, and VTI Technologies, rely on Eyelit as a trusted software partner.
About Infotonics Technology Center (www.infotonics.org)
The Center establishes R&D and other strong collaborative programs among industry, university, and government in order to develop and commercialize innovative MEMS-based photonic and microsystems technologies; provides world-class MEMS-based photonic and microsystems prototyping and pilot-line production facilities and infrastructure; and supports high-tech businesses and enhances an environment in which they thrive.
Eyelit Inc.
Amy DeHeer
+1 905 502 6184


text:
11 *Incredibly* Easy 5-Ingredient Pastas for Quick Weeknight Meals
When Sunday night rolls around, trying to plan dinners for the week can be exasperating. After all, there are only so many sheet pan dinners your family can handle! But one thing the fam never seems to tire of are noodles. And between all of those speedy 15-minute pasta dishes and one-pot wonders, there's a doable recipe out there for ANY hectic scenario. Whether you're short on time or simply lacking ingredients, these 11 nom-worthy recipes will come to the rescue with five ingredients or less.
Roasted Garlic Pasta
When you've got long tender noodles, roasted garlic, black pepper, and cheesy Asiago goodness, all seems right in the world. Not only does this recipe call for minimal ingredients, but it's only 15 minutes from pot to plate. (via Girl Gone Gourmet)
Five-Ingredient Simple Green Pasta Salad
Scrumptious pasta tubes and chopped-up green beans are tossed in a fab basil sauce to create a deliciously lean pasta salad. (via Pinch of Yum)
5-Ingredient Mediterranean Pasta Salad
If you love options, you'll fancy this bountiful salad boasting minimal yet flavorful ingredients like sun-dried tomatoes and garlicky olives. Serve it as a side or mow down a plateful for dinner. (via Real Food Whole Life)
Easy Chicken Enchilada Tortellini Bake
With tender cooked chicken, spicy red pepper diced tomatoes, and a Mexican cheese blend, this garlicky cheese tortellini bake is comfort food at its best. (via This Gal Cooks)
Five-Ingredient Cherry Tomato and Ricotta Linguini
This tasty and refreshing summertime pasta brings burst cherry tomatoes to your plate and swaps common mozzarella for fluffy dollops of light ricotta. Sign us up. (via Cooking for Keeps)
One Pan Lemon Garlic Pasta
Bright and lemony linguini is elegant enough to be a Sunday meal, yet easy enough to be a weeknight wonder; you're one pan and 20 minutes away from pasta perfection. (via Yellow Bliss Road)
Sausage Asparagus Pasta Bake
A cheesy and hearty pasta bake is always a great idea — especially when it's brimming with asparagus, Italian sausage, stretchy mozzarella, and whole grain penne for a nutritional punch. (via Buy This Cook That)
Five-Ingredient Butternut Squash Alfredo
Thanks to roasted butternut squash, you can enjoy a creamy and silky Alfredo-esque sauce without the guilt. Hit it with some salt, pepper, and Parm, and you're golden. (via Cooking for Keeps)
Five-Ingredient Chicken Feta Pasta
Fill your plate with whole wheat fettuccini, chicken, tomatoes, tangy feta, and fresh basil for an easy summertime meal you and your fam will *love.* (via Well Plated by Erin)
Five-Ingredient Tomato and Artichoke Penne
When you've got soft marinated artichokes, tomatoes, leafy spinach, and freshly grated Parm, your penne doesn't need anything else. (via Eating With Emmy Lou)
Four-Ingredient Spaghetti Carbonara
Some pastas are so good, they only need four ingredients — and that's exactly the case with this simple yet delectable carbonara with bacon, egg yolks, and Pecorino. (via Cooking for Keeps)


text:
No sales found matching your criteria.
Try removing some filters on the left side of this page. Or you can increase your search distance.
Below are sales from nearby areas.
There will be a plethora of items including: House decorations, appliances, furniture, toys, clothes, stereo equipment, workout equipment, shelving units, storage units, games, clothes rack, DVD players, TVs, Etc. etc.… → Read More
GMC truck has engine rebuilt (Shuts auto), undercarriage powdered, new bumper, Cab needs work, etc. All the parts are here. Dismantled by renter but never put back together. Best offer by calling Mike Sullivan, 307-630-5362.… → Read More
Huge Garage Sale In Church Gym
Aug. 2 AND Aug. 4 CLOSED AUG. 3. Location at Cheyenne SDA Church Gym - 723 Storey Blvd. Antique radio/record player, small chest freezer, pressure cooker, steamer, guitar new, portable clothes washer, and much more!… → Read More
Large garage sale. Tools, garden tools, hoses, breakfast table and 2 chairs, cherry cabinet, 2 oak chairs, paint equipment, shelf supports, storage cabinet, oak worktop, filing cabinet, weaving equipment and yarn, rug yarn, tapestry loom, shuttles, warping frame, household items, clamps, and much, much more… → Read More
Household goods, toys, kid pool, tools, bikes, refrigerator and more… → Read More
Neighborhood Sale. One Day Only.
Multi-family sale. Valuable building tools, TREK bike, mahogany wardrobe, weights, Scandinavian cane chairs, Sleeping bags, backpacks, snowshoes, skis, boots, conga drums, lamps, golf clubs and shoes. Many items from family estate.… → Read More
We are having a Yard Sale on Oct. 19th-20th (Saturday and Sunday) from 8am – 2pm on both days. Located at 1002 S. Summit View Dr., Fort Collins, CO 80524, about one mile west of I-25. Items include electronics, furniture, household items, appliances, toys, clothing, 2 vehicles (2010 Audi Q7 and 1998 Volvo V70), and much more.… → Read More
New/barely used furniture + home decor, rugs, antique pieces, tile and more!… → Read More
Book's like, western, fantasy, kids, mystery,fiction,thriller/suspense, etc. $6 a bag. Bags provided. Piano. CD'S, celtic, pop, rock, classical, more. Some collectibles. .75 cents to $4 each. Comic's apart from Book Sale.$1 each. DVDs, family, Nut Job,I Am Legend, more. $1 to $2 each. Kitchen Appliances smaller ones like crock pot, blender,… → Read More
Books Comic's Dvds Cds Records Clothes More! Soda store brands .50 cents each. Water in bottles .75 cents each, Cold in ice. All prices fair.… → Read More
YardSales.net is the fastest growing yard sale site, with 0 sale photos and 0 garage sales in Carr, Colorado. YardSales.net offers you the best list of Carr yard sales every week.


text:
THE most important game of the season so far will take place this Sunday in Parke.
The Kilmeena Senior team will take on Knockmore in a "do or die" relegation play off. Defeat will see Kilmeena playing Junior B Championship football next year!
Despite the disappointment of losing to Ballina, the lads should go into this game full of confidence from their outstanding league form. If they can perform as they did in the recent win against Achill, who were undefeated until facing Kilmeena, it should ensure we maintain our Junior status.
Apart from the Ballina game, the team has performed well and are creating chances in every game. Narrow defeats and not converting scoring opportunities have left us in this position. This team has the skill and they are good enough to win, they just need to perform on the day. As always your support for our team could prove vital in securing victory, please come and shout for your team.
Junior Football Championship Relegation Play-Off
Sunday 31 August. Venue; Parke GAA Pitch. Time; 1pm.
Kilmeena GAA v Knockmore


text:
Big Hairy Dog Information Systems is a premier support company for Retail Pro.
For more than 26 years Retail Pro Software has been a leading Inventory Control & POS package for specialty retail and web store operations across the globe.
RPRO can be found in over 52,000 retail stores worldwide, in more than 90 countries & 19 different languages. It is one of the only mid-tier global systems with multi-currency & multi-tax as well as multi-subsidiary features on the market today.
Retail Pro Software is a leader in Inventory Control with the flexibility to handle both hard-goods and soft-goods extremely well.


text:
Could anyone give me the idea to setup?
Thank you.
This board has been archived, no new registrations are allowed. Please come join us on our discord!
Need help ! How can i setup the Bot like grid system?
General Discussion about Haasbot's Trade Server Bot. (All)
1 post • Page 1 of 1
1 post • Page 1 of 1
Return to "Trade Bot Discussion"
Who is online
Users browsing this forum: No registered users and 1 guest


text:
Adjusting the Controls of Your TENS 7000
Your shiny new TENS 7000 device's controls are so simple even a little kid could use them (remember to keep this unit from the reach of children, though). Once you get the hang of how to adjust them, obtaining pain relief will be as easy as 1-2-3.
First off, there is the output. Turn the amplitude control dial clockwise for either channel 1 or 2 until you hear a beeping sound. Select the mode, rate and width prior to increasing the amplitude.
The stimulation mode is set by pressing on the 'mode' button. The stimulation mode selected has to be that which your doctor has recommended for your particular case. Your physician may go as far as pre-setting it for you.
The pulse rate set function is entered by pressing the 'set' button and then increasing or decreasing (using the correspondingly labeled buttons) pulse rate to the levels your doctor has instructed you to use.
You can adjust the pulse width 50-300us in 10us increments by pressing the 'set' button and increasing and decreasing pulse width to the desired levels, very much like you did with the pulse rate.
The timer can be adjusted from 5 to 90 minutes, or continuous in increments of 5 minutes. That is, a cycle consisting of 5 to 9o minutes to continuous and back to 5 minutes. The clock icon flashes once per second during the final 5 minutes.
Back to channel amplitude, turn either channel clockwise. Output indication will be displayed on the LCD screen; on the left for channel 1 and on the right for channel 2. The channel amplitude knob should be gradually turned until your doctor-recommended setting is reached. The same goes for the other channel, should the two channels be used.
Keep in mind that the stimulation levels suggested by your doctor are not set in stone. If you feel uncomfortable during application, you don't have to endure it just because the good doctor said so. What you have to do is reduce the intensity and tell your physician about it.
Once a therapy session is over, both channels should be turned back all the way to the 'off' position. The electrode wires are to come off next, held by the plug rather than the cord. The actual electrodes may be left on if treatment is to be continued shortly after. Otherwise they must be removed and cleaned with mild soap and water, as should be the skin as well.


text:
When Alice wants to send Bob some coins all she has to do is sign a transfer of ownership with her private keys and publicize that announcement until Bob is happy with the number of witnesses. For small transactions, Bob might be happy with a couple dozen witnesses but with large value transactions he'd want thousands of heavy peers to witnesses the transaction. Why?
Bob's coins only belong to him if someone on the network can provide proof to everyone else, on demand, that those coins belong to Bob. If all of Bob's witnesses go offline then there's no one to vouch for him! In such a scenario, Alice would be able to transfer the ownership of those coins again since no one would be there to stop her.
This is basically turning the current popular algorithms upside down since you're not flooding the network with transaction data, you're listening to the network to make sure no one is trying to take your coins. As long as you yourself are online ready to provide proof, the transfer of ownership signed by Alice, then no one can take your coins away from you. Ideally, you'd have many peers ready to provide that proof, not just you in case you go offline and in exchange you either pay them a small fee or you also host others' proofs ready to serve them up to whomever asks for it.
The only attack I can think of is Alice announces the transfer of ownership of coins to Bob and announces another transfer of ownership of the same coins to Mallory at the same time and hopes that Bob and Mallory don't hear each other. But if Bob and Mallory are smart they'll query enough peers such that their queries overlap and collide in at least one peer on the network.
So to wrap up: using witnesses to 'hear' the transfer of ownership and at least one of those witnesses ready to defend the transfer of ownership is enough to prevent double spends. Caveats are that you want enough witnesses to hear the transfer and you want at least one witness always online. In bitcoin, your funds are safe even if go offline but here your funds are only safe if you're online 24/7. Otherwise you need to trust 3rd parties to defend your coins for you. Are there any attack vectors with this? It sounds pretty simple, too good to be true? Consensus is too slow and we don't need consensus here. And you don't need synchronization since everything would be dynamic.
Can this Proof of Witness be attacked in a way I'm not seeing?


text:
The other day, I found an article from the New York Times about Arthur Aron's experiments in intimacy, and how the writer (Mandy Len Catron) had tried it for herself. The idea is that, given the right conditions, it is possible to make two strangers fall in love by going through a 3-stage conversation, followed by a 4-minute gaze into each other's eyes. (The articles all say "stare silently". I like "gaze" better.) As the article says, "The 36 questions in the study are broken up into three sets, with each set intended to be more probing than the previous one."
This sounded a lot like the products offered by various pick-up artists that promise "You can make any woman want to be your girlfriend", if you just follow their "tried and tested" techniques and sequences. I am sceptical about such things. I am also sceptical that they are unique, which is the usual sales pitch: "no one else has come up with this stuff before". If a psychology experiment from 20+ years ago came up with it first, paying a PUA for it is a mug's game!
Catron reports that it does work, and she fell in love with her partner for the evening. But the crucial point was, "Love didn't happen to us. We're in love because we each made the choice to be." This is because (as she remarks near the head of the piece), "I see now that one neither suggests nor agrees to try an experiment designed to create romantic love if one isn't open to this happening."
That last point is one reason why PUA claims are pretty much bullshit, of course.
Today, in my daily feed email from them, I find that the Guardian picked up the story and asked two "guinea pigs" to try the experiment themselves and report back:
Bim Adewunmi: Written down, it doesn't seem like much, but once required to think about these things – and so quickly – it becomes intense.
The usual route to intimacy is, among other things, winding and often accidental. This thing we are doing, in a largely empty restaurant, is deliberate and accelerated. But as the evening goes on, what was originally discomfiting becomes almost euphoric release.
The questions are probing – your most embarrassing moment, your favourite memory etc – and the great thing about them is how they force reflection. Not looking at the questions beforehand was a good idea, because I think I would have cooked my answers a bit.
Clearly, this is for work, and I imagine we are both squirrelling away quotes for our respective pieces. But I also made the effort to wear contacts – not my spectacles – and applied shaky eyeliner. I put on lipstick, dammit. At the very least, I was open to meeting someone romantically. Acknowledging that gives me a jolt.
– – –
Archie Bland: You are, briefly, on the floor marked "early flirtation", and the one marked "endearing second-date revelation"; the trouble is, there's no way of getting off. Nor does it seem to stop at "totally into each other" or "madly in love", either, nor even "watching boxsets and only communicating in grunts". Without really noticing it, we finally come to rest at "old friends with a slightly complicated history that they avoid talking about". It's not that I suddenly want to go out with Bim (or, I am pretty certain, she with me); it's that I feel like I already have, and it meant a lot, but it's definitely over. And we haven't even got to the stare-off yet.
Both said that they had revealed things to the other that their best friends didn't know yet. I thought of how sometimes internet exchanges have seemed intimate in this way for me, and how cautious I have learned to be about that sort of thing. I thought of the potential for using such a technique for grooming (had Arthur Aron derived from first principles the engineered solutions that those who groom others use?) Because I am, ultimately, a worrier, and a writer, and therefore trained to see the dark side in most things.
I also thought about the implications for my own haphazard attempts to find love. Could I use the concepts here to derive my own version of those PUA bollocks, or else use it to analyse what I've been doing so far?
So, I sat down and looked at the list of questions to see if (as usual) I'm unusual; to see if I could see a pattern in my (particularly online/dating site) interactions and which questions came up; and to see if I could draw any conclusions or wisdom from considering them. This means, to some extent, trying to imagine the conversation in which the questions arise (rather than just reading them off the screen), which may twist the results somewhat.
As it's outlined, there are three levels of intimacy: Set I is the lowest and Set III the highest.
Set I should be the least vulnerable. But 7, 9, 10, and 12 in Set I are questions I would be very guarded about answering. Questions 3 and 6 would also feel very vulnerable to say to just anyone. Question 11 would be a big fat "nope" in a face-to-face situation. The scary thing is that it is possible some or more of these answers can be derived from my blogging so far. I think some of the answers would kill a date stone dead, if I answered honestly ("What are you most grateful for?" "I didn't commit suicide when I suffered from depression" – yeah, that's gonna lift the mood!)
It's possible that being asked some of these questions and giving guarded answers, would just train me to be more guarded for the later questions. For example, Q.11: "Take 4 minutes and tell your partner your life story in as much detail as possible" is an instant flag for me that I will choose which parts are okay to tell and which not. After that point, the openness is gone.
Some of the questions are things that I think about a lot anyway, so I have ready answers. Reading them, or imagining being asked them, made me smile with the familiarity and comfort of knowing the answers without even thinking. Not all of them are answers I feel okay with at "level 1" – for instance, being genderfluid, there's an obvious answer to, "If you could wake up tomorrow having gained any one quality or ability, what would it be?" Rereading the question, I think maybe they want something less physical (and less superpower-y), but I don't get that chance to reassess in a conversation. (It's also a question I would not answer openly, and would take some thought over). My point being, that's something that I need to have a certain level of intimacy already, before I talk about it one-to-one.
For Set II, the first thing I noticed was "What is the greatest accomplishment of your life?" This has been one of my favourite questions to ask in an opening message on dating sites, because I believe it will tell me something about their values and life experiences, which in turn will help me gauge how easy it will be to get along with them. If it counts as a "level 2" intimacy, then maybe I'm going in way too fast? Indeed, several of the questions in Set II are among those highlighted as being good openers either in speed-dating or to address in a dating website profile (or opening message):
- What do you value most in a friendship?
- What is your most treasured memory?
- What does friendship mean to you?
- What roles do love and affection play in your life?
This implies that the people designing the advice are aware of intimacy but are perhaps accelerating it a little bit too quickly; while it's clear to see the importance of some of these questions in gauging "compatibility" (whatever we mean by that) and shared goals from dating, at the same time the implication from this structure is that they are questions for a second date, or later on on a first date, than for an opening (even if you have shared stuff on your profile already).
The questions mentioned already are, of course, ones for which I have (by virtue of thinking about dating website advice and suchlike) already considered answers. The "greatest accomplishment" one especially – since I lead with it, I generally offer a reply as well.
I wondered if any Set II questions felt to me as though I would be more comfortable with them than some of those in Set I. The obvious one for me was, "If a crystal ball could tell you the truth about yourself, your life, the future or anything else, what would you want to know?" I think because most of the things that matter to me I either know, or am happier not knowing. There's one question about my past that intrigues me, though, and it's something easy for me to give up but that implies intimacy and naughtiness: "Did the first girl who offered to have sex with me mean it on any level, or was she purely teasing?" I think I've mentioned the incident on this blog before. I wonder about the "what-if" of that situation, but it's so long ago now that the answer doesn't matter. It's an answer that (a) implies vulnerability and (b.) talks about sex, so (c.) gives the impression of intimacy. But in actual fact, it's as easy for me as, "Before making a telephone call, do you ever rehearse what you are going to say?" ("Yes, because I want to be sure I get everything in and I can be quite forgetful if I don't")
Anything that asks about relationships to others (family, or mother – the last two) immediately make me defensive, because those are other people and without context and a much deeper conversation, I would not share that without professional-client confidentiality, at least.
Which leads me to my thoughts on Set III. Some of these questions are really intense. While "How do you feel about your relationship with your mother?" from Set II is the stereotypical psychotherapist question, several of the questions in Set III are questions that I might expect to come up at some point in a counselling or therapeutic relationship. Let's face it, Q. 36 is the basis of counselling/therapy in a nutshell: "Share a personal problem and ask your partner's advice on how he or she might handle it. Also, ask your partner to reflect back to you how you seem to be feeling about the problem you have chosen." (The "ask their advice" is less relevant to counselling especially, but my understanding is that clients often do ask it; the answers come from the second part.)
Interestingly, there are still one or two that I would move up to Set II, or even Set I, because they are things I care about and am open about. For example, "What, if anything, is too serious to be joked about?" and perhaps even, "Share with your partner an embarrassing moment in your life." – the latter is another staple of dating site/speed-dating advice, and of course, I have thought in the past about how to answer.
Some offer themselves to defensive answers: "Complete this sentence: 'I wish I had someone with whom I could share …'" allows the glib, "My last chocolate," for example. And yes, that was my instinctive reaction when I saw this question. I would probably try to laugh it off in a similar way in person, too.
The factor that makes me think I might not be quite so defensive about these questions is from the Guardian article: "In the end, the waiter has to kick us out; we are the last people in the restaurant. Admittedly, this is more because there are so many questions to get through … It is nearly midnight" The exercise takes hours to complete, and I bond well when given that amount of time with just one person as my focus. It's like introvert socialising heaven! I do feel more relaxed towards the end, and less likely to be guarded, if I spend that sort of time sat with someone and sharing little emotional-communicative indulgences. I still think some of these questions are ones that would jolt me out, or have me feeling vulnerable in a threatened rather than intimate way.
The conclusions I have reached so far are to do with myself, and perhaps others who have reasons to be cautious around others. Survivors, or people outside of the "acceptable" ways of being (e.g. sex workers, or former sex-workers) might very well have reasons to think differently about the intimacy ratings of questions like these. Some of them are like landmines – for instance, my "grateful" answer is an unexpected explosion underfoot – because of the associations they have.
I also noticed that many of the questions, particularly at level 2, are the sorts of things that I would use to decide whether this was a person I wanted to fall in love with or not. It is one thing to say, "Yes, I am open to the idea of falling in love." It is another to say, "I am interested in this person (and might want to fall in love with them)" (as Catron reveals was probably the case for her and her acquaintance). I am at once cautious and foolhardy with my heart in these matters. And so, I am cautious (and foolhardy) with some of these questions, too.
I want to come back to this, and see if I can construct a way of using the concepts in dating. For instance, does it hint at ways to construct a good profile? First message? and so on.
Can I, perhaps, come up with questions to fit in Set I to replace the ones that I disliked? What about Set II or Set III? What does it mean about my chances, that my sense of intimacy is perhaps not quite in sync with others'?
More research needed…


text:
Port of Baltimore to Receive $105M in Federal Funding
U.S. Senator Barbara A. Mikulski, Vice Chairwoman of the Senate Appropriations Committee, has just announced that the fiscal year (FY) 2017 Energy and Water Development funding bill includes $105 million to invest in the Port of Baltimore.
In the next step of the appropriations process, the bill will move to the Senate floor this week.
"From the dock workers who unload the ships to the manufacturers who rely on cargo transported by the ships, the Port means jobs. Through construction jobs and 'The Corps' dredging to prepare the port for the bigger ships that will soon be sailing through the widened Panama Canal, we are supporting jobs today and jobs tomorrow," Senator Mikulski said.
The bill includes $20.575 million for annual maintenance dredging of the Port of Baltimore shipping channels, a major economic engine for America and Maryland, supporting more than 25,000 jobs across the state – including 14,630 direct jobs.
Projects for the Port of Baltimore receiving federal funding include:
- $20.575 million for annual maintenance dredging of the Port of Baltimore's 50-foot shipping channels making it one of the few East Coast ports deep enough to accommodate the super container ships coming through the Suez Canal and, beginning next year, from the Panama Canal. The channel goes up the Chesapeake Bay from the Virginia Capes to Fort McHenry, a distance of 175 miles.
- $21.622 million for the C&D Canal for annual maintenance dredging of its shipping channels, which serve as a shortcut for vessels traveling between Baltimore and points north. Approximately forty percent of the Port of Baltimore's shipping traffic goes through the C&D Canal that connects the Chesapeake Bay with the Delaware River.
- $62.3 million for the continuation of the Poplar Island environmental restoration project, which is taking clean dredged materials from the shipping lanes leading to the Port of Baltimore and using it to stabilize the shoreline, create habitat area and restore the wetlands of one of the Chesapeake Bay's most valuable island ecosystems off Talbot County. Poplar Island is the Port's only operational dredge placement site. Without the expansion, either the Port will be forced to overload the existing site, which will compromise the environmental benefits of this project, or the Corps would reduce its annual dredging of the Port's channels.
- $325,000 for Baltimore Harbor drift removal to remove trash and debris from the Port of Baltimore's shipping channels.
The bill also includes $50 million equally divided between donor ports and energy transfer ports as authorized in the Water Resources Reform and Development Act of 2014 (Public Law 113-121). Collectively, these twenty ports are responsible for more than 60 percent of the total Harbor Maintenance Tax collection each year.
Harbor Maintenance Trust Fund
The bill provides approximately $1.3 billion for the Harbor Maintenance Trust Fund. This exceeds the target in the Water Resources Reform and Development Act (Public Law 113-121).
Across the country, a backlog of dredging projects forces vessels to carry less than their capacity, threatens to close harbors and increases the risk of vessel grounding. While dredging is the Harbor Maintenance Trust Fund's primary activity, other functions include construction and maintenance of dredge material disposal areas, jetty repair and operation activities.
The Harbor Maintenance Trust Fund supports USACE Baltimore District's annual dredging of the Wicomico River, navigation projects that serve Eastern Shore watermen and projects in the Baltimore Harbor and channels.


text:
Blight Tower
Welcome to Blight Tower! Your goal is to destroy the monstrous BLIGHT TOWER! Stand under OUTPOSTS to capture them and spawn an army of DEADLY CAPSULES! Be warned, the BLIGHT TOWER has a powerful BLAST ATTACK that does heavy damage and turns your DEADLY CAPSULES against you! Don't die!
So where's the incorporation of the 'Sacrifice' theme, you ask? Simple: If you find yourself running low on HEALTH, shoot rockets at a nearby DEADLY CAPSULE. There's a chance it will drop HEALTH for you upon death. Be advised, running out of DEADLY CAPSULES will lose you the game!
If you've played a shooter in the 20 [twenty] years, you'll pick this up no problem. Otherwise, it's W, A, S, and D to move, mouse to aim, and left click to shoot your shot.
Full disclosure: While the game is technically feature complete, I ran out of time to work on my wishlist of audio and visual deliverables (note: the DEADLY CAPSULES). Many assets are still placeholders. There are also several bugs, as of writing. I have opted out of the Audio category, as the only sound in the game is for the rocket launcher. I have also opted out of the Humor category as it doesn't fit the theme of the game. I did opt into graphics as the models are custom made and the level design is (mostly) done.
All this to say, I pushed myself more than any other Ludum Dare yet. My project scope was more than I could handle in the end, but I'm still incredibly proud of the results. Thank you for checking out Blight Tower and being an integral part of the Ludum Dare experience :)
Tools used:
- Unity
- ProCore
- Blender
- VS Code
|Windows, HTML5
|Made with
|Unity, Blender, GIMP
|Ludum Dare, Ludum Dare 43
|Average session
|A few minutes
|Ludum Dare
Click download now to get access to the following files:
Development log
- Blight Tower - NOW WITH FEWER BUGS!Dec 10, 2018
Leave a comment
Log in with itch.io to leave a comment.


text:
Here is a little sneak peek of my Wedding! I'm working on a big Wedding post showing you guys all the photos but here is a little teaser for now 😉 We Eloped so there was not a big wedding and no wedding plans! We said the "I Do's" three day's after getting the Marriage license (and we had an amazing family gathering/ceremony last Saturday, a week after our Wedding, organized by Joey's family!) Three days.. however short, is still three days 😉 and we spend those day's creating the perfect fun little Elope Wedding Day! (We Eloped like a pro) One of the best parts was finding this little white bride dress for the puppy at Petco and getting pre-ceremony puppy cudles. (ps. puppy cuddles involve Puppy teeth) xoxo
8 Comments on A Sneak Peek of my Wedding Day
Aw congrats! Can't wait to see more pictures! xx
Looking forward to see some photos 🙂 x
Can't wait to see more!
Congratulations on tying the knot! <3 It sounded like a really fun and cozy wedding 🙂 Can't wait to see more photos! xx
Congratulations on finding that someone special! Love those Roger Vivier shoes. Can't wait for you to share those precious moments of some of the best days of your life!
So beautiful!! I'm waiting for moree!!!:)
Beautiful shoes
Congratulations!happy for you guys!;)


text:
By this author
Culture September 08, 2018
Filmmaker Lynne Ramsay's musical inspiration
Film director and screenwriter Lynne Ramsay frequently turns to books to find engaging stories, as with her adaptation of Jonathan Ames' novella You Were Never Really Here, but it's music that truly inspires her. "Sometimes you need to explain that sound is the bigger picture. I'm a frustrated musician at heart, because the mix to me is one of the most exhilarating parts to making a film."


text:
The number of international students grows every year at undergraduate and posgradute level.
Student Fairs
The Department of Computer Science has two active research groups, Ríomh and Sigma. Also included is a link to reseach output by staff in the department.
- Nigeria Fairs April 2019Join us in in Abuja on the 4th October and in Lagos on the 6th October 2018.


text:
Your institution must subscribe to JoVE's Biology section to access this content.
Fill out the form below to receive a free trial or learn more about access:
Caenorhabditis elegans is a useful model to explore the functions of polyunsaturated fatty acids in development and physiology. This protocol describes an efficient method of supplementing the C. elegans diet with polyunsaturated fatty acids.
Cite this ArticleCopy Citation | Download Citations | Reprints and Permissions
Deline, M. L., Vrablik, T. L., Watts, J. L. Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans. J. Vis. Exp. (81), e50879, doi:10.3791/50879 (2013).
Translate text to:
Fatty acids are essential for numerous cellular functions. They serve as efficient energy storage molecules, make up the hydrophobic core of membranes, and participate in various signaling pathways. Caenorhabditis elegans synthesizes all of the enzymes necessary to produce a range of omega-6 and omega-3 fatty acids. This, combined with the simple anatomy and range of available genetic tools, make it an attractive model to study fatty acid function. In order to investigate the genetic pathways that mediate the physiological effects of dietary fatty acids, we have developed a method to supplement the C. elegans diet with unsaturated fatty acids. Supplementation is an effective means to alter the fatty acid composition of worms and can also be used to rescue defects in fatty acid-deficient mutants. Our method uses nematode growth medium agar (NGM) supplemented with fatty acidsodium salts. The fatty acids in the supplemented plates become incorporated into the membranes of the bacterial food source, which is then taken up by the C. elegans that feed on the supplemented bacteria. We also describe a gas chromatography protocol to monitor the changes in fatty acid composition that occur in supplemented worms. This is an efficient way to supplement the diets of both large and small populations of C. elegans, allowing for a range of applications for this method.
Fatty acids are essential structural components of membranes as well as efficient energy storage molecules. Additionally, fatty acids can be cleaved from cellular membranes by lipases and be enzymatically modified to produce signaling effectors1. Naturally occurring polyunsaturated fatty acids (PUFAs) contain two or more cis double bonds. The omega-3 fatty acids and the omega-6 fatty acids are distinguished from each other based on the positions of double bonds with respect to the methyl end of the fatty acid. Healthy diets require both omega-6 and omega-3 fatty acids. However, Western diets are particularly rich in omega-6 fatty acids and poor in omega-3 fatty acids. A high omega-6 to omega-3 fatty acid ratio is associated with increased risk of cardiovascular and inflammatory diseases, however, the precise beneficial and detrimental functions of specific fatty acids are not well understood2. The roundworm Caenorhabditis elegans is useful in studying fatty acid function because it synthesizes all of the enzymes necessary to produce a range of omega-6 and omega-3 fatty acids, including an omega-3 desaturase, an activity that is absent in most animals3,4 . Mutants lacking fatty acid desaturase enzymes fail to produce specific PUFAs, leading to a range of developmental and neurological defects4-6.
To study the physiological effects of dietary fatty acids, we have developed a biochemical assay compatible with genetic analysis using both mutant and RNAi knock-down techniques in C. elegans. Supplementation with specific PUFAs is achieved by adding a fatty acid sodium salt solution to the agar medium prior to pouring. This results in PUFA uptake by the E. coli food source, where it accumulates in the bacterial membranes. C. elegans ingest the PUFA-containing bacteria, and this dietary supplementation is sufficient to rescue the defects of PUFA-deficient mutants. Supplementation of most fatty acids has no detrimental effects on wild type animals, however, specific omega-6 fatty acids, especially dihomo-gamma linolenic acid (DGLA, 20:3n-6) cause a permanent destruction of C. elegans germ cells7,8 .
Gas chromatography is used to monitor the uptake of the supplemented fatty acid in the bacterial food source (either OP50 or HT115) as well as in the nematodes. The addition of the detergent Tergitol (NP-40) in the media allows for even distribution of fatty acids through the entire plate and more efficient uptake of the fatty acids by the E. coli and the nematodes. We have found that unsaturated fatty acids are readily taken up by bacteria and C. elegans, but the uptake of saturated fatty acids is much less efficient. This article will describe step-by-step how to supplement the agar media with fatty acids, as well as how to monitor fatty acid uptake in the nematode using gas chromatography.
Subscription Required. Please recommend JoVE to your librarian.
Polyunsaturated fatty acids are sensitive to heat, light and oxygen. Therefore, care must be taken when preparing fatty acid supplementation plates such that fatty acids are not exposed to excess heat and light. NGM media containing 0.1% Tergitol (NP-40) is autoclaved and partially cooled, after which fatty acid sodium salts are added with constant stirring. The plates are allowed to dry in the dark. Uptake of fatty acids by C. elegans cultured on these plates can then be monitored by gas chromatography.
1. Preparation of Fatty Acid Supplemented Media
- Measure out media components into an appropriately sized flask. Per 1 L, add 17 g Bacto-agar, 2.5 g tryptone, 3 g NaCl, 1 ml 5 mg/ml cholesterol dissolved in ethanol, and 10 ml 10% Tergitol dissolved in water.
- Add 80% of final desired volume of Millipore water and autoclave the media along with empty glass bottles (equal to the number of different fatty acid concentrations to be tested), as well as appropriate graduated cylinders.
- Cool media in a water bath set to 55 °C. While the media is cooling prepare the working stock solution of the fatty acid sodium salt by breaking open the glass vial, using safety precautions and ensuring glass particles do not mix in with the fatty acids. Weigh out enough fatty acid to make a 100 mM working stock.
- Bring the fatty acid solution to a final concentration of 100 mM with purified water. Fully dissolving the fatty acid sodium salt typically takes about 20-30 min. Purge the working stock of fatty acid with argon or nitrogen, because contact with an inert gas prevents oxidation. Cap the vial and store the working stock in the dark until media has cooled.
- (Optional) If RNAi media is desired, measure ampicillin and Isopropyl β-D-1-thiogalactopyranoside (IPTG) solutions here.
- When the agar has cooled to 55 °C add per 1 L: 1 ml of 1 M MgSO4, 1 ml of 1 M CaCl2, and 25 ml of phosphate buffer (108.3 g KH2PO4 and 35.6 g K2HPO4 per 1 L autoclaved). Add the filter sterilized ampicillin and IPTG solutions if making RNAi plates. For all types of plates, add sterile water to bring the final media volume to 1 L.
- Near a flame, transfer media to an autoclaved and appropriately sized graduated cylinder and then add warm sterile water to desired volume. Transfer media back to initial flask and mix by stirring.
- Near a flame, transfer media into a number of bottles equal to the number of concentrations to be tested by measuring with an autoclaved and appropriately sized graduated cylinder. Maintain the aliquoted media as liquid using a water bath until the fatty acid working stock has fully dissolved.
- Place one bottle on a stir plate and stir until the media is warm to the touch, but not hot. Make sure to leave yourself enough time to stir in the fatty acid stock solution and pour plates before the media begins to solidify. If stir plate has temperature control, set it to 55 °C.
- Dilute the 100 mM fatty acid working stock into the media for the final concentration desired. Stir media until the white precipitate is in solution (approximately 1 min). Media may still be slightly cloudy afterwards.
- (Optional) If RNAi media is desired add ampicillin to 0.1 mg/ml and IPTG to 2 mM final concentration.
- Pour media using an automated pipette aid and a sterile 25 ml pipette, adding 8 ml of media per 60 mm plate or 25 ml of media per 100 mm plate. Repeat fatty acid addition and plate pouring steps for the remaining bottles.
- After the agar has solidified, cover the fatty acid-supplemented plates with a box or store at room temperature in a well-vented drawer to protect from light oxidation.
- Seed E. coli OP50 onto plates two days after plates have dried. For 60 mm plates, pipette 300 µl of an overnight OP50 culture (grown at 37 °C). If RNAi plates were poured, seed with 300 µl of RNAi bacteria after the plates have dried for four days. Plate drying time may need to be adjusted due to differences in humidity in various lab environments.
- Incubate the plates in a dark environment at room temperature while the bacterial lawn is drying.
2. Inducing Germ Cell Destruction by Supplementation of DGLA
- C. elegans grown on plates containing 0.3 mM DGLA (20:3n-6) become sterile due to the destruction of germ cells in both larval stage and adult nematodes.
- Prepare a synchronized population of L1 larvae by treating gravid hermaphrodites with alkaline hypochlorite solution(for a 10 ml solution: 0.5 ml of 5M NaOH, 2.5 ml of household bleach, and 7 ml H2O). Gently rock the gravid hermaphrodites in this solution until the adult worms dissolve. Eggs will be preserved, and can be pelleted by low speed centrifugation.
- Wash egg preparation 3 times in M9 buffer (3 g KH2PO4, 6 g Na2HPO4, 5 g NaCl, 1 ml 1 M MgSO4, H2O to 1 L. Add MgSO4 after autoclaving). To provide enough oxygen, resuspend the eggs in a 15 ml plastic tube to a final volume of 5 ml of M9 buffer and rock on a shaker overnight.
- L1 larvae should be added to the supplemented plates two days after seeding with OP50, or one day after plating the RNAi bacteria. Incubate worms at 20 °C for three days or until they reach the adult stage.
- Adult worms can be scored for sterility using a dissecting microscope with high enough magnification to visualize embryos developing in the uterus. Successful germ cell loss will appear as a clear uterus void of eggs.
- Worms can also be scored by fixing and staining with the nucleic acid dye diamindinophenylindole (DAPI), which facilitates the visualization of nuclei in germ cells and developing embryos. A rapid DAPI stain is achieved by picking worms into a drop of M9 buffer on a watchglass9.
- Flood watch-glass with 1 ml of a 0.2 ng/m. DAPI in 95% ethanol solution, let sit for approximately 5 min.
- Pick worms into a drop of VectaShield on a slide, and then cover with a coverslip.
- Seal coverslip and store at 4 °C in the dark overnight for optimum staining, however slides can also be viewed immediately. Score for presence or absence of germ cell nuclei using a fluorescence microscope equipped with a UV lamp and filter.
3. Confirming Fatty Acid Uptake by Gas Chromatography
Overall fatty acid composition of C. elegans can be determined by producing fatty acid methyl esters (FAMEs) which are then separated and quantified using gas chromatography4.
- Collect 500-1,000 adult worms by washing them off of the plates with water and transferring to a silanized 13 mm x 100 mm glass screw top tube.
- Let worms settle by gravity, and then remove as much water as possible with a glass Pasteur pipette.
- Wash worms once with water, and then again remove as much water as possible.
- FAMEs are formed by adding 1 ml of 2.5 % H2SO4 in methanol, and then heating at 70 °C for 1 hr in a water bath.
- Remove the tubes from the water bath and cool for 1 min.
- Extract FAMEs by adding 1.5 ml water and 0.25 ml of hexane.
- Recap tubes and shake vigorously.
- Centrifuge tubes in a tabletop clinical centrifuge for 1 min at maximum speed to separate hexane from aqueous solvent.
- Transfer hexane (top layer) to a GC vial insert within a GC vial, being careful not to transfer any of the aqueous phase. For GC analysis, 1-2 μl of FAMEs in hexane is injected onto a polar capillary gas chromatography column suitable for FAMEs analysis. The Agilent 7890 GC injector is set at 250 °C, with a flow rate of 1.4 ml/min, and the GC oven is programmed for an initial temperature of 130 °C, which is held for 1 min. Subsequently, the temperature is ramped 10 °C/min until 190 °C, and then ramped again at 5 °C/min until 210 °C and held for an additional 1 min.
- To ensure that uptake of DGLA has occurred, analyze FAMEs by flame ionization detection (FID) or mass spectrometry (MS) detection, using authentic standards for the identification of the C. elegans fatty acids.
Subscription Required. Please recommend JoVE to your librarian.
Representative Results
Supplementation of the C. elegans diet is limited by the ability of the bacterial food source to uptake and incorporate fatty acid into the bacterial membrane. To determine the ability of E. coli OP50 to assimilate various fatty acids into its membranes, OP50 was plated onto media with no supplement, 0.1 mM and 0.3 mM concentrations of stearic acid (18:0), sodium oleate (18:1n-9), and sodium DGLA (20:3n-6). Plates were dried at room temperature for 2 days in the dark, and incubated at 20 °C for 3 days. Bacterial lawns were collected by gently scraping the lawn into water with a flame-sterilized spatula. Bacteria were pelleted by centrifugation, and treated with 2.5% H2SO4 in methanol to produce fatty acid methyl esters, which were analyzed by GC/MS following the methods listed in the Procedure, step 3. The results demonstrate that unsaturated fatty acids (oleate and DGLA) incorporate into OP50 in higher amounts than the saturated fatty acid stearic acid (Figure 1A).
Additionally, L1 stage N2 larvae were grown on the same batch of supplemented plates and harvested after three days growth at 20 °C. Worms were washed off of the plates and fatty acids in total worm preps were analyzed by GC/MS. The change in supplemented fatty acids is graphed in Figure 1B. These studies demonstrate that supplementation of saturated fatty acids does not change the relative amount of saturated fatty acids in worm tissues, while supplementation of unsaturated fatty acids increased the relative amounts of unsaturated fatty acids in C. elegans lipids. Taken together, the data shown in Figure 1A and Figure 1B demonstrate that the relative accumulation of supplemented fatty acids in C. elegans correlates directly with the relative accumulation of fatty acids in the dietary E. coli.
We have previously shown that dietary DGLA causes sterility in C. elegans10. Figure 2 illustrates the dose response of DGLA induction of sterility in C. elegans. The concentration of DGLA in worm lipids in which 50% of the population will be sterile is approximately 12%. Interestingly, the response to DGLA can be altered by genetic mutations in C. elegans. A recent finding is that the insulin growth factor-dependent stress pathways can suppress the DGLA-induced germ cell destruction8. Supplementing the diet of worms containing deleterious mutations in either the daf-2 insulin/IGF receptor, daf-2(e1370), or the daf-16/FOXO transcription factor, daf-16(mu86), illustrates the usefulness of this method to unravel genetic pathways that influence the physiological effects of dietary fats. Synchronized L1 larvae were pipetted onto DGLA supplemented media. After 3-4 days of growth, worms were scored for sterility, as determined by the absence of eggs in the uterus of adult worms. DGLA supplemented daf-2(e1370)mutants were fertile, with little to no induced germ cell loss compared to wild type (N2) worms at both 0.15 mM and 0.3 mM supplementations (Figure 3).In contrast, DGLA supplemented worms with inactive FOXO (daf-16(mu86)) displayed a higher percentage of sterile worms compared to wild type when fed on plates containing 0.15 mM DGLA (Figure 3).
Figure 1. Uptake and incorporation of supplemented fatty acids by E. coli OP50 and C. elegans. A. E. coli OP50 was grown on plates containing 0.1 mM or 0.3 mM stearic acid, sodium oleate, or sodium DGLA as well as un-supplemented plates. After five days of growth on plates at 20 °C, E. coli were harvested and fatty acid methyl esters were generated for analysis by GC/MS. Because OP50 does not produce oleic acid or DGLA, and produces only trace amounts of stearic acid, the percentage of each supplemented fatty acid in the E. coli lipids reveals the ability of OP50 to incorporate the supplemented fatty acid. Error bars are SD. B. Change in C. elegans fatty acids in young adults grown for three days, starting at L1 stage, on E. coli plates containing 0.1 mM or 0.3 mM stearic acid, sodium oleate, or DGLA. The values for change in stearic acid and DGLA were obtained by subtracting the relative amount of 18:0 or 20:3 in worms grown on supplemented plates from those of worms grown on unsupplmented plates. To monitor uptake of oleic acid, the sum of oleic acid plus downstream C20 PUFAs (20:3, 20:4n-6, 20:4n-3, and 20:5) were calculated in supplemented and unsupplemented plates, because incorporated oleic acid is further desaturated and elongated. Error bars are SD. Click here to view larger image.
Figure 2. Increasing concentrations of DGLA in worm lipids correlate with increasing sterility in C. elegans. Wild type (N2) worms were treated with various concentrations of DGLA. The % DGLA in total worm lipids and the % of the population that is sterile is plotted for is plotted for 17 data points from five independent feeding experiments using dietary DGLA concentrations ranging from 0-0.3 mM DGLA. Click here to view larger image.
Figure 3. Physiological effects of supplementing C. elegans with DGLA. Starved L1 larval wild type, daf-2(e1370), or daf-16(mu86) were plated onto un-supplemented, 0.15 mM or 0.3 mM DGLA supplemented media and grown to the adult stage. At least 150 individual worms were then scored for sterility. Thedaf-2(e1370) mutants were almost completely fertile, even at 0.3 mM DGLA, while thedaf-16(mu86) mutants display an increased number of sterile worms compared to wild type at 0.15 mM DGLA. Error bars are SEM. Click here to view larger image.
Subscription Required. Please recommend JoVE to your librarian.
Here we describe a method of supplementation of C. elegans with dietary unsaturated fatty acids. As mentioned above, care must be taken in the preparation of PUFA supplemented plates because the reactive nature of the double bonds in PUFAs causes these fatty acids to be sensitive to oxidation through heat and light11. To avoid oxidation, it is important to add the PUFA to the liquid agar medium after the media has cooled to 55 °C and stores plates in a dark environment.
Others have introduced free fatty acids to C. elegans using a DMSO or ethanol carrier, or else by directly adding fatty acids by microinjection into the vulva12-14. Partial rescue of mutant phenotypes was achieved by fatty acid supplementation of growth plates without the use of Tergitol(NP40)14-15. There are apparently a variety of effective ways to introduce fatty acid into C. elegans, although it is difficult to compare the efficiency of various methods because, in most experiments, the uptake of the fatty acids in the nematodes was not monitored. We find that our method allows consistent and efficient supplementation of unsaturated fatty acid to population sizes of a few nematodes to tens of thousands.
We have found it essential to monitor the uptake of supplemented fatty acids in C. elegans to aid in the interpretation of experimental outcomes. For example, the uptake of fatty acid by the bacteria food depends on the E. coli strain used as a food source for the nematodes. We have found that OP50 takes up greater amounts of unsaturated fatty acids than E. coli strains HT115, HB101, or NA22, strains commonly used for feeding RNAi experiments (HT115) or used for high density nematode growth (HB101 and NA22). Because of the variation in uptake of certain fatty acids, it is important to test several concentrations of fatty acid and to monitor uptake using gas chromatography. Most labs have achieved rescue of mutant phenotypes using fatty acid concentrations in the range of 0.08-0.2 mM5,6,15-18, however, effects on lifespan have been reported with supplementation of arachidonic acid at a concentration as low as 0.01 mM14, and others have used doses as high as 0.6 mM for rescue of a phenotype induced by feeding RNAi using the HT115 E. coli strain19. In our hands, supplementation of E. coli OP50 with very high concentrations of PUFAs, greater than 0.4 mM, result in excessive dietary PUFA accumulation in worm lipids, up to 50% of total fatty acids. This leads to nonspecific slow growth and abnormalities of the vulva.
A limitation of the technique is the inability to efficiently supplement with saturated fatty acids. Solubility issues at the time of adding the supplement to the media as well as the inefficient uptake and integration of saturated fatty acids in E. coli leave this method more suitable for supplementing unsaturated fatty acids (Figure 1).
Our method provides reproducible supplementation of mono- and polyunsaturated fatty acids, including fatty acids not normally produced by C. elegans, such as docosahexaenoic acid (DHA, 22:6n-3). Growth and neurological defects in PUFA-deficient mutants such as fat-3 can be rescued by relatively low levels of dietary PUFA5. Fatty acid supplementation can be used by researchers who wish to alter the fatty acid composition of C. elegans by dietary means in order to study a range of physiological processes.
Subscription Required. Please recommend JoVE to your librarian.
The authors declare that they have no competing financial interests.
We thank Chris Webster for performing the preliminary experiments shown in Figure 3 of the representative results and Jason Watts and Chris Webster for helpful comments on the manuscript. Funding for this study was provided by a grant from the National Institutes of Health (USA) (R01DK074114) to JLW. Some nematode strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440).
|Catalog Number
|J.T. Baker
|(5 mg/mL in ethanol)
|J.T. Baker
|J.T. Baker
|J.T. Baker
|J.T. Baker
|Sodium dihomogamma linolenate
|Warm sterile Millipore water
|Sterile water for collecting worms
|Nuclease-free Water for DGLA stock solution
|Fisher Scientific
|100 mg/ml in water (for RNAi plates)
|Isopropyl-beta-D-thiogalactopyranoside (IPTG)
|Gold Biotechnology
|1 M in water (for RNAi plates)
|J.T. Baker
|Fisher Scientific
|Fisher Scientific
|diamindinophenylindole (DAPI)
|Vector Laboratories
|Glass Flask
|Autoclaveable Glass bottles with stirbars
|Autoclaveable Glass Graduated Cylinder
|Stir Plate
|Waterbath at 55+ °C
|Precision Scientific Inc.
|Screwcap Brown Glass Vial
|Sun SRI
|200 494
|Argon gas tank
|Automated Pipette aid
|Sterile Serological Pipettes (25 ml)
|Bunsen Burner
|Dissection microscope
|Silanized glass tube
|Thermo Scientific
|for FAMEs derivitization
|PTFE Screw caps
|Clinical tabletop centrifuge
|GC Crimp Vial
|SUN SRi
|200 000
|GC Vial Insert
|SUN SRi
|200 232
|GC Vial cap
|SUN SRi
|200 100
|Gas Chromatograph
|Mass Spectrometry Detector
|Column for gas chromatography
|30 m x 0.25 mm fused silica capillary column
- Haeggstrom, J. Z., Funk, C. D. Lipoxygenase and leukotriene pathways: biochemistry, biology, and roles in disease. Chem. Rev. 111, 5866-5898 (2011).
- de Lorgeril, M., Salen, P. New insights into the health effects of dietary saturated and omega-6 and omega-3 polyunsaturated fatty acids. BMC Med. 10, 50 (2012).
- Spychalla, J. P., Kinney, A. J., Browse, J. Identification of an animal omega-3 fatty acid desaturase by heterologous expression in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 94, 1142-1147 (1997).
- Watts, J. L., Browse, J. Genetic dissection of polyunsaturated fatty acid synthesis in Caenorhabditis elegans. Proc. Natl. Acad. Sci. U.S.A. 99, 5854-5859 (2002).
- Watts, J. L., Phillips, E., Griffing, K. R., Browse, J. Deficiencies in C20 Polyunsaturated Fatty Acids Cause Behavioral and Developmental Defects in Caenorhabditis elegans fat-3 Mutants. Genetics. 163, 581-589 (2003).
- Kahn-Kirby, A. H., et al. Specific Polyunsaturated Fatty Acids Drive TRPV-Dependent Sensory Signaling In Vivo. Cell. 119, 889-900 (2004).
- Brock, T. J., Browse, J., Watts, J. L. Genetic regulation of unsaturated fatty acid composition in C. elegans. PLoS Genet. 2, e108 (2006).
- Webster, C. M., Deline, M. L., Watts, J. L. Stress response pathways protect germ cells from omega-6 polyunsaturated fatty acid-mediated toxicity in Caenorhabditis elegans. Dev. Biol. 373, 14-25 (2013).
- Kadyk, L. C., Lambie, E. J., Kimble, J. glp-3 is required for mitosis and meiosis in the Caenorhabditis elegans germ line. Genetics. 145, 111-121 (1997).
- Watts, J. L., Browse, J. Dietary manipulation implicates lipid signaling in the regulation of germ cell maintenance in C. elegans. Dev. Biol. 292, 381-392 (2006).
- Pryor, W. A., Stanley, J. P., Blair, E. Autoxidation of polyunsaturated fatty acids: II. A suggested mechanism for the formation of TBA-reactive materials from prostaglandin-like endoperoxides. Lipids. 11, 370-379 (1976).
- Kniazeva, M., Shen, H., Euler, T., Wang, C., Han, M. Regulation of maternal phospholipid composition and IP(3)-dependent embryonic membrane dynamics by a specific fatty acid metabolic event in C. elegans. Genes Dev. 26, 554-566 (2012).
- Edmonds, J. W., et al. Insulin/FOXO signaling regulates ovarian prostaglandins critical for reproduction. Dev. Cell. 19, 858-871 (2010).
- O'Rourke, E. J., Kuballa, P., Xavier, R., Ruvkun, G. omega-6 Polyunsaturated fatty acids extend life span through the activation of autophagy. Genes Dev. 27, 429-440 (2013).
- Taubert, S., Van Gilst, M. R., Hansen, M., Yamamoto, K. R. A Mediator subunit, MDT-15, integrates regulation of fatty acid metabolism by NHR-49-dependent and -independent pathways in C. elegans. Genes Dev. 20, 1137-1149 (2006).
- Lesa, G. M., et al. Long chain polyunsaturated fatty acids are required for efficient neurotransmission in C. elegans. J. Cell Sci. 116, 4965-4975 (2003).
- Brock, T. J., Browse, J., Watts, J. L. Fatty acid desaturation and the regulation of adiposity in Caenorhabditis elegans. Genetics. 176, 865-875 (2007).
- Goudeau, J., et al. Fatty acid desaturation links germ cell loss to longevity through NHR-80/HNF4 in C. elegans. PLoS Biol. 9, e1000599 (2011).
- Yang, F., et al. An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis. Nature. 442, 700-704 (2006).
0 Comments
Post a Question / Comment / Request
You must be signed in to post a comment. Please sign in or create an account.


text:
J. David Bournazian
- J.D., University of San Diego School of Law, Editor, California Regulatory Law Reporter
- B.A., Rutgers University
- California
- Member, The Association of Business Trial Lawyers of Orange County
- Member, The American Institute of Architects
- Member, Armenian Bar Association
- Member, Orange County Bar Association, 22 years
- Board Member, Laura's House
J. David Bournazian is a trial litigator and advisor and is often called upon by clients to handle their most difficult and sensitive matters. Bringing a depth of experience in handling highly intricate litigation and business negotiations, David represents national and international clients, including well known and publicly traded companies, in both litigation and transactional matters. Businesses seek out his assistance in crafting their trial strategies when facing especially difficult litigation.
David has served as the general counsel for Frank Gehry and Gehry Partners, LLP, one of the most renowned architects in the world, and for a highly successful Fortune 50 international retail and accessories company.
David has also served as lead trial counsel in cases involving business, real estate, class action, complex construction, Proposition 65, corporate, coverage, and employment. His complex commercial litigation practice is focused on disputes, with an emphasis on real estate, construction and ADA challenges. He has represented a wide variety of construction projects including airports expansions, large retail developments, sports arenas, bridges and highways, university and government buildings, power plants, hospitals, and mixed-use towers. He also has wide-ranging experience in class action litigation and regulatory issues regarding consumer products, involving a variety of state, federal and international environmental, product safety, chemical management, and related regulatory issues.
Government Service
- Finance Management, The White House, Executive Office of the President of the United States, 1989
- Lead trial lawyer on proposition 65 cases throughout the State of California.
- Lead trial attorney for national construction claims, including complex development matters for an international retailer.
- Defended class action claims involving the alleged taking of private information by a national retailer and even obtained a client recovery.
- Lead trial attorney on numerous coordinated actions involving the Los Angeles International Airport.
- Defended class action claims and class certification attempts, including numerous wage and hour class action cases.
- Obtained defense award in internationally publicized fraud and discrimination case.
- Recovered millions for a developer of a power plant, including performance bonuses based upon efficiency of operation of the power plant.
- Obtained a defense outcome for the developer of a public works project, which included False Claims Act allegations.
- Negotiated design development agreement for new landmark museum in Paris, France.
- Obtained multimillion-dollar result in intellectual property case involving application of foreign law.
- Pursued several insurance claims to verdict and resolution at state and federal court levels, including complex contribution and recovery claims.
- Negotiated licensing agreement for $400 million professional sports arena in New York.
- Defended national retailer in website ADA accessibility claims.
- Defended hundreds of access claims involving countertops, aisle ways, and point of sale stations.
- Attained published result in McLaurin v. Russell Sigler, Inc., 155 F.Supp.3d 1042 (2016).


text:
Most of the energy consumption in the United States comes from fossil fuels. Natural gas, petroleum, and coal have accounted for 81.5 percent of the nation's energy consumption for over 100 years, according to the U.S. Energy Information Administration. However, burning fossil fuels has been attributed to atmospheric pollution, global warming, the release of toxins into the environment, and health problems. Only by reducing dependency on these can such issues be mitigated. Fortunately, there are many ways to reduce the reliance on fossil fuels – here are 20 of them you can do actively.
Decrease energy consumption by using more efficient fluorescent light bulbs, which last a long time. The U.S. Department of Energy says these use 25 to 35 percent less energy than traditional incandescent bulbs and can work for about 10 times as long. Compact fluorescent lamps (CFLs) are popular for household fixtures, and fluorescent tube and circline lamps are ideal for commercial settings and garages.
Turn off the lights when out of a room for more than 15 minutes, whether using CFLs, halogen, or incandescent lighting. The power saved reduces demand on electric utilities, which in turn require less output from power plants that burn fossil fuels.
Turn off the television, computer, or any other electrical device when it's not in use; this is a good way to conserve energy and help these investments last longer, so you don't have to buy new ones as soon.
Use appliances with the ENERGY STAR label, which is only placed on products that meet high-efficiency standards. To inform consumers, the label usually displays a calculation of annual savings as a percentage or monetary value. It can be found on air conditioners, heaters, refrigerators, and just about any home appliance.
Ditch fossil fuels. Get clean energy at no cost.Check availability
Reuse products that require fossil fuel resources to produce. By avoiding paper or plastic shopping bags, you can reduce the demand on the production process, which is fossil fuel intensive. Instead, use fabric bags for groceries. The amount of plastic and Styrofoam used can be limited by purchasing glass plates and cups, which can be reused over and over again instead of throwing them out every time, using new ones, and having to purchase more in bulk.
Purchase goods manufactured with recycled materials. This helps to reduce the demand for fossil fuels as well. Check for product labels that indicate such materials are included. Reusing anything that can be, such as printer ink cartridges, helps too.
Recycle. By recycling waste, less of it goes to landfills. Many cities have recycling programs and require residents to put plastics, paper, and aluminum into separate trash bins. You can also donate clothing, lights, and appliances you don't plan to use anymore, instead of throwing them out, so they continue to serve a purpose rather than investing in additional manufactured products. According to GreenAmerica.org, just over 34 percent of U.S. waste is recycled or composted, but the EPA estimates 75 percent of all waste can be recycled.
Avoid using printing inks that are petroleum-based. Soy ink, derived from soybeans, is an eco-friendlier choice and can be found in printer cartridges. Newspapers and other printed publications use it as well, some since the late 1980s.
Fossil fuels are a major part of the oil refining process, which is how gasoline is made. Instead of driving, consider taking public transportation, which reduces how many cars are on the road and contributing emissions. You could also carpool, ride a bicycle, or walk.
Telecommuting eliminates the need to travel altogether, avoiding the use of fossil fuels and the costs of fuel and travel. All you need is a computer, Internet connection, and smart phone.
Avoid using nylon, which is petroleum-based. It can be found in luggage, life vests, and umbrellas. Even nylon clothing or clothing made of polyester is fossil fuel reliant, but clothes made of natural fibers are a more economically and environmentally friendly choice.
Plastics are costly on the environment to produce and dispose of and do not decompose. Bottled water products consume a lot of plastic, so reusable containers filled with filtered tap water are better instead. Biodegradable plastics will break down over time and are a somewhat better option if you would rather stick to plastic.
For a headache, drink more water as the cause is often dehydration and the fact that over-the-counter products such as Aspirin are made with oil products.
Waxing the floors will keep them looking shiny and new, but commercial waxes are often made from petroleum derivatives. Beeswax is a natural alternative and can do the job just as well.
Natural essential oils are popular, and not only for their attractive and pleasant scents. Traditional scent products and even perfumes are often made with petrochemicals, in turn feeding the demand for fossil fuels and increasing their effects on the environment.
Wood decks may increase the demand for tree-based products, but plastic materials like those made to resemble wood are made from oil. Using natural wood helps ease the dependence on fossil fuels.
Ditch fossil fuels. Get clean energy at no cost.Check availability
Soft contact lens wearers often don't know their products are made using petroleum products. Although eyeglass frames require some oil to make, they typically last much longer and can be donated once no longer suitable for the wearer.
Instead of replacing your roof with tar, which is a byproduct of coal production, use metal or clay tiles that are not so heavily fossil fuel dependent.
Power your home with solar energy, so it's not necessary to buy it from coal plants. Plus, energy from the sun and the electricity solar systems produce, are free.
More environmentally friendly gardening practices can reduce demand on fossil fuels as well. These include using pushable mowers and planting gardens that limit the amount of space that must be cut. Reducing your reliance on fossil fuels can lead to more attractive landscaping on your property, not to mention be better for the environment, your wallet, and health.


text:
Throughout the interview, note the characteristics of the patient's speech, including the following:
Quantity. Is the patient talkative or relatively silent? Are comments spontaneous or only responsive to direct questions?
Rate. Is speech fast or slow?
Loudness. Is speech loud or soft?
Articulation of Words. Are the words spoken clearly and distinctly? Is there a nasal quality to the speech?
Fluency. This involves the rate, flow, and melody of speech and the content and use of words. Be alert for abnormalities of spontaneous speech such as these:
Slow speech of depression; accelerated rapid, loud speech in mania
Dysarthria refers to defective articulation. Aphasia refers to a disorder of language. See Table 16-2, Disorders of Speech, p. 600.
■ Hesitancies and gaps in the flow and rhythm of words These abnormalities suggest apha sia. The patient may have so much
■ Disturbed inflections, such as a monotone difficulty in talking or in understand ing others that you may not be able
■ Circumlocutions, in which phrases or sentences are substituted for a word to obtain a history. Y°u may als° the person cannot think of, such as "what you write with" for "pen"
falsely suspect a psychotic disorder.
■ Paraphasias, in which words are malformed ("I write with a den"), wrong ("I write with a bar"), or invented ("I write with a dar").
If the patient's speech lacks meaning or fluency, proceed with further testing as outlined in the following table.
Testing for Aphasia
Word Comprehension
Repetition Naming
Ask the patient to follow a one-stage command, such as "Point to your nose." Try a two-stage command: "Point to your mouth, then your knee."
Ask the patient to repeat a phrase of one-syllable words (the most difficult repetition task): "No ifs, ands, or buts."
Ask the patient to name the parts of a watch.
Ask the patient to read a paragraph aloud.
These tests help you to decide what kind of aphasia the patient may have. Remember that deficiencies in vision, hearing, intelligence, and education may also affect performance. Two common kinds of aphasia—Wernicke's and Broca's—are compared in Table 16-2, Disorders of Speech, p. 600.
A person who can write a correct sentence does not have aphasia.
Was this article helpful?
Your heart pumps blood throughout your body using a network of tubing called arteries and capillaries which return the blood back to your heart via your veins. Blood pressure is the force of the blood pushing against the walls of your arteries as your heart beats.Learn more...


text:
@StephBernaba: Me and Holiday Baking Championship #HolidayBakingChampionship https://t.co/sMiC104oht
@mringram Holiday baking championship is on @FoodNetwork https://t.co/J5Ha9ez690
Listen, the @FoodNetwork Holiday Baking Championship makes the world a better place. https://t.co/nLXxmmNhnp
Me patiently waiting for Halloween Baking Championship and Halloween Wars to start on @FoodNetwork https://t.co/s0KcqWNamm
How I feel when I see @JessePalmerTV's @FoodNetwork Holiday Baking Championship commercials in October https://t.co/Nb7YPFSGHt
KIDS BAKING CHAMPIONSHIP COMES BACK TONIGHT!!!!! 🎂🍰🍮🍩 @FoodNetwork @duffgoldman https://t.co/XWW4AJodOO
It's Monday night, give me all of your @FoodNetwork holiday baking shows please! https://t.co/AhnFdjiXUQ
Needed a stove and real ingredients while watching Kids Baking Championship tonight. Watch out #FoodNetwork https://t.co/pWhQgSJxnk
First day of fall = 🎃. Watch @FoodNetwork's Halloween Baking Championship on Philo. https://t.co/LcGGXWobS2
A fresh batch of holiday bakers has arrived! #HolidayBakingChampionship @FoodNetwork https://t.co/JMnq4i8AJo https://t.co/MvT90bygUv
@FullerFarmer @FoodNetwork Woohoo
I personally think my HOLIDAY CUPCAKES would win😘 #HolidayBakingChampionship https://t.co/3RndmKe1dZ
You know it is the holiday season when #holidaybakingchampionship is back on @FoodNetwork. https://t.co/vAiv4Qfb1m
@KHONnews: Hawaii keiki competes on Food Network's 'Kids Baking Championship' https://t.co/Zen8uTYVl8 https://t.co/TeOhq01wTc
Kid Baking Championship! Donuts anyone? #foodnetwork #kidsbakingchampionship #Donuts #izzielovestobake https://t.co/706rSlwkz9
Winner !!! Kids Baking Championship season 5! @baking_with_nat Wolfiesmom duffgoldman @FoodNetwork https://t.co/67apWlFlSn
[email protected] what's up with the blackface for Halloween Baking Championship? https://t.co/GOvtt9n98g
Championship round next week yeah they had me fucked 💪🏾 https://t.co/d1fZ5GnRAG
Watching Halloween Baking Championship on @FoodNetwork with the foster kittens https://t.co/v8CawPm6TI
Go behind-the-scenes of tonight's festive family baking challenge! #HolidayBakingChampionship https://t.co/JQdZZGvBSJ
Holiday lazy baking 🍰 #foodporn https://t.co/XTLDeKJ8gG
@Ball_Durham: Me as Quinn Cook was walking to get his NBA Championship ring https://t.co/4flLpFwwQe
Easy Pecan Brownie Recipe| Holiday Baking (Holiday recipes) https://t.co/mTGMu3ZC7w https://t.co/fDjieiDhrY
11 Holiday Cooking and Baking Hacks https://t.co/8pSCuM1EJn https://t.co/5tzZHfGw6X
Me baking and cooking in this heat https://t.co/5XFyhSBKzQ
Someone busted me in a #selfie moment on the set of #holidaybakingchampionship 😂 @FoodNetwork https://t.co/OwToRIyTey
"I love everything pumpkin" it's official @LilGranolanBar is me #HolidayBakingChampionship @FoodNetwork https://t.co/2EUO6nFsDy
Post in 2018 Holiday Cooking and Baking https://t.co/W9Qg5ViRAd #cooking #food #discussion https://t.co/LKqnQatfSP
Bank holiday baking! 🍰🍓
#cake #chocolate🍫 #strawberry🍓 #homemade #baking #foodie #cakesofinstagram #getcreative https://t.co/nriuvx0Wbg
@ghetto004: A concept: me, on holiday, eating good food and minding my business https://t.co/tVAfMbN2gF
I love @JohnnyGWeir and @taralipinski on @FoodNetwork's Wedding Cake Championship 🎂 https://t.co/sxQbJFaoZy
This product uses Twitter API but is not endorsed by Twitter.


text:
Twenty-eight of the UK's top prop-making and movie FX artists are coming together for a very good cause. Each artist has exclusively customized a bust featuring their own unique take on the Frankenstein monster. Each bust will later go to auction where 100% of the proceeds will be donated to the Make-A-Wish Foundation in the UK. An amazing project, and a worthwhile cause.
We here at Second Union are extremely enthusiastic to have been chosen to do an exclusive reveal of the second of twenty-eight unique Frankenstein inspired busts. We hope you enjoy what you see, and we hope that you continue to follow, like and check back with them often via the variety of social media links at the bottom of the page.
Introducing: "Bad to the Bone" by UK Prop Builders & Crossley Custom Inc. Airbrushing
More About THE MONSTER CHARITY PROJECT 2017
Some of the UK's top prop-making and movie Special FX artists will customize unique designs of Frankenstein's Monster to raise money for Make-A-Wish UK.
Frankenstein's Monster as you have never seen him before!
28 of the UK's top prop-making, sculpting and SPFX make-up artists have been brought together to take part in a unique charity project.
Each artist has been given a bespoke Frankenstein-inspired monster bust, created and supplied by SVFX University of Bolton Special & Visual Effects team, with the simple instruction: Customise him into any design you like.
Once completed, the artists will then donate the finished busts back to Make-A-Wish UK, who will then auction off the busts via their online worldwide auction site.
All the artists and the back room project crew donate their time and skills totally free of charge and 100% of the proceeds raised will go to Make-A-Wish UK.
Last year the same project team raised a staggering £28,000 for Make-A-Wish at Star Wars Celebration London with a similar project featuring an iconic Star Wars Helmet. 30 artists took part creating such custom designs as Deadpool, Judge Dredd, Batman and a Studio Ghibli-inspired design.
The busts will be going on a UK tour during 2017 taking in such conventions as Horror Con UK and will feature at other events TBA.
In addition to the above conventions, people will be able to start viewing the completed busts from May 2017 via various social media outlets.
The project busts then go to auction on October 31st, 2017, ending November 5th, 2017
Reveal Number One: "Frankenfly"
For Further information and updates, you can follow the project and all its progress at:
Press Contact:
Ryan Holloway: firstname.lastname@example.com


text:
Content Integrity
Stay ahead of spam and scams
Protect your community from malicious content – before it gets posted.Request a demo
Protect your community from malicious content – before it gets posted.Request a demo
Keep bad content off your platform, so you can grow your user base and increase engagement.
Reduce fraudulent content by up to 90% with technology that defends in real time.
Create better customer experiences, reduce churn, and preserve your brand's integrity.
Slash long review queues by 60% or more with powerful workflow automation.
The Sift engine is built to accelerate growth, while protecting your business from bad actors.
Bolstered by the largest global network of data in the industry, our real-time machine learning looks at more than 16,000 signals to identify good and bad users with unparalleled accuracy – so you can stop new attacks before they happen.Learn how we're different
Messages sent in the last hour: 34
Email domain: yopmail.com
Posts created in the last day: 35
Users with the same browser + IP: 5
Users with the same cookie: 6
Everything your team needs to secure user accounts and work more efficiently.
Get critical information to review risky users and content in one place, for maximum efficiency and speed.
Uncover networks of fraudsters via connection visualizations, and easily block fraud rings en masse.
Automate content moderation with workflows that block or accept users and content, or route risky behavior to review.
Gain visibility into user activity and fraudulent content to spot trends in real-time and make better decisions.
More than 60% of our deactivations are handled by Sift, mostly automated. We've also noticed fewer fake accounts, a drastic reduction in spam accounts, and faster removal of duplicate accounts.
After integrating with Sift, our time to resolution has gone down 10x. What used to take hours and days now takes minutes. Sift has saved each of our engineers a few hours a day.
Our partnership with Sift has really allowed us to focus on growth and enable our community to have a secure and trusted environment where they can share their personal style.
Sift is intuitive, user-friendly, and eliminates the need for hours of human analysis and review. The knowledge of Sift's global network makes our machine learning model highly accurate.
Your information will be used to contact you about our service and subscribe you to our direct marketing communications. You can, of course, unsubscribe at any time. Please see our Website Privacy Notice.


text:
IMPORTANT UPDATE: We're temporarily adjusting store hours to better serve customers and the community in response to COVID-19.
Dear Budget Wichita Customer,
As a valued member of the Budget family, we appreciate the trust you place in us as your rental car partner. Whether you are renting a vehicle at the airport or one of our neighborhood locations, we are here for you.
We are closely monitoring the coronavirus (COVID-19) situation and its impact on our local community and the world at large. Know that we are following the guidance of government and health authorities, and are doing all we can to protect our employees and customers.
To that end, we want to highlight a few of the precautionary measures we are reinforcing and implementing:
Sanitation and Cleanliness-We are regularly disinfecting all of our facilities, ensuring that counters and commonly touched surfaces are thoroughly wiped down. All vehicles are cleaned prior to rental with particular focus on the dashboard, steering wheel, door handles, and gear shifts.
Team Member Health-We are encouraging our employees to practice frequent hand hygiene and asking those who exhibit symptoms of illness to remain at home and contact their physician.
We recognize this is a challenging and uncertain time for everyone. If you are looking for an alternate mode of travel, consider renting one of our vehicles. We're happy to help.
If you have an upcoming reservation, we look forward to serving you. If you would like to book a new reservation or if you need to modify/cancel an existing reservation, you can do so by visiting our website.
Thank you for your continued loyalty as we navigate these evolving circumstances together.
Budget Wichita Management
Please wait a moment...


text:
I'm going to attempt to paraphrase a story I heard at a small-groups conference a little over a year ago, so please bear with me. The conference was the 2007 Purpose-Driven Small Groups gala at Saddleback Church, and the speaker was Randy Frazee. The story centered on Frazee's first attempt at small-group ministry as the senior pastor of a large church in suburbs of Fort Worth, Texas.
Being the senior pastor, Frazee wanted to make sure that his small group was an example of excellence for the rest of the church to follow. So after much prayer and deliberation, he and his wife invited the most spiritual couple in the church to serve as co-leaders, and then the most athletic, most attractive, most intelligent, and most wealthy couples to round out the group. They called it their "Super Small Group."
The only problem was that the couples' homes were spread out over several miles, which meant that Frazee and his wife had to travel between 25 and 40 minutes each way to get to their group meeting every week. After a while, the added time (and added money spent on childcare) began to take its toll. Still, the Frazees persevered, and the Super Group settled into a regular and comfortable routine.
About this time, however, Frazee's new next-door neighbor—a real whiz at hospitality and socializing—began organizing regular get-togethers within the neighborhood. Soon, the little community was coming together at least once a week to play games, share food, chat, and pretty much experience a suburban version of Acts 2:42 (minus the selling of property, of course). Consequently, Frazee's Super Small Group became less and less appealing in light of what was happening in his own back yard.
He described ...